LOCUS       DAVKGC010000001        58642 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKGC010000001 DAVKGC010000000
VERSION     DAVKGC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58642
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.001"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(107..856)
                     /locus_tag="VW049_000001"
     CDS             complement(107..856)
                     /locus_tag="VW049_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9431207.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            989..3325
                     /locus_tag="VW049_000002"
     CDS             989..3325
                     /locus_tag="VW049_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU9431208.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEIATLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3427..4104
                     /locus_tag="VW049_000003"
     CDS             3427..4104
                     /locus_tag="VW049_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9431209.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4091..4888
                     /locus_tag="VW049_000004"
     CDS             4091..4888
                     /locus_tag="VW049_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9431210.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4905..6008
                     /locus_tag="VW049_000005"
     CDS             4905..6008
                     /locus_tag="VW049_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9431211.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            6309..7328
                     /gene="galE"
                     /locus_tag="VW049_000006"
     CDS             6309..7328
                     /gene="galE"
                     /locus_tag="VW049_000006"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9431212.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            7330..8283
                     /locus_tag="VW049_000007"
     CDS             7330..8283
                     /locus_tag="VW049_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9431213.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(8345..8551)
                     /locus_tag="VW049_000008"
     CDS             complement(8345..8551)
                     /locus_tag="VW049_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9431214.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            8603..9079
                     /locus_tag="VW049_000009"
     CDS             8603..9079
                     /locus_tag="VW049_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9431215.1"
                     /translation="MAKEPWQEDIYDQEESRAGHRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            9088..9759
                     /locus_tag="VW049_000010"
     CDS             9088..9759
                     /locus_tag="VW049_000010"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9431216.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            9890..10228
                     /locus_tag="VW049_000011"
     CDS             9890..10228
                     /locus_tag="VW049_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9431217.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            10231..10860
                     /locus_tag="VW049_000012"
     CDS             10231..10860
                     /locus_tag="VW049_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9431218.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            11100..12251
                     /locus_tag="VW049_000013"
     CDS             11100..12251
                     /locus_tag="VW049_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9431219.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            12564..13313
                     /gene="truA"
                     /locus_tag="VW049_000014"
     CDS             12564..13313
                     /gene="truA"
                     /locus_tag="VW049_000014"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9431220.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            13303..14076
                     /locus_tag="VW049_000015"
     CDS             13303..14076
                     /locus_tag="VW049_000015"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9431221.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            14054..14515
                     /locus_tag="VW049_000016"
     CDS             14054..14515
                     /locus_tag="VW049_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9431222.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            complement(14774..14977)
                     /locus_tag="VW049_000017"
     CDS             complement(14774..14977)
                     /locus_tag="VW049_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9431223.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(14970..16052)
                     /locus_tag="VW049_000018"
     CDS             complement(14970..16052)
                     /locus_tag="VW049_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9431224.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(16178..16960)
                     /locus_tag="VW049_000019"
     CDS             complement(16178..16960)
                     /locus_tag="VW049_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9431225.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(16960..18303)
                     /locus_tag="VW049_000020"
     CDS             complement(16960..18303)
                     /locus_tag="VW049_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9431226.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILEAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(18402..19718)
                     /locus_tag="VW049_000021"
     CDS             complement(18402..19718)
                     /locus_tag="VW049_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9431227.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYLEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGTFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            19912..21138
                     /locus_tag="VW049_000022"
     CDS             19912..21138
                     /locus_tag="VW049_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9431228.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILAGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            21337..22911
                     /locus_tag="VW049_000023"
     CDS             21337..22911
                     /locus_tag="VW049_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9431229.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            23175..23963
                     /gene="codY"
                     /locus_tag="VW049_000024"
     CDS             23175..23963
                     /gene="codY"
                     /locus_tag="VW049_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9431230.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            23963..24538
                     /locus_tag="VW049_000025"
     CDS             23963..24538
                     /locus_tag="VW049_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9431231.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            24620..24705
                     /locus_tag="VW049_000026"
     tRNA            24620..24705
                     /locus_tag="VW049_000026"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24654..24656,aa:Leu,seq:aag)
     gene            24988..26118
                     /gene="ugpC"
                     /locus_tag="VW049_000027"
     CDS             24988..26118
                     /gene="ugpC"
                     /locus_tag="VW049_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9431232.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(26265..26411)
                     /locus_tag="VW049_000028"
     CDS             complement(26265..26411)
                     /locus_tag="VW049_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431233.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            26619..27377
                     /locus_tag="VW049_000029"
     CDS             26619..27377
                     /locus_tag="VW049_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9431234.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            27465..27977
                     /locus_tag="VW049_000030"
     CDS             27465..27977
                     /locus_tag="VW049_000030"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9431235.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            28159..29103
                     /locus_tag="VW049_000031"
     CDS             28159..29103
                     /locus_tag="VW049_000031"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9431236.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            29112..29789
                     /locus_tag="VW049_000032"
     CDS             29112..29789
                     /locus_tag="VW049_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9431237.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTSQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            29887..30645
                     /locus_tag="VW049_000033"
     CDS             29887..30645
                     /locus_tag="VW049_000033"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9431238.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            30683..32188
                     /gene="lytC"
                     /locus_tag="VW049_000034"
     CDS             30683..32188
                     /gene="lytC"
                     /locus_tag="VW049_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9431239.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDFN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            32685..33203
                     /locus_tag="VW049_000035"
     CDS             32685..33203
                     /locus_tag="VW049_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9431240.1"
                     /translation="MVELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            33333..33839
                     /locus_tag="VW049_000036"
     CDS             33333..33839
                     /locus_tag="VW049_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9431241.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            34041..35273
                     /gene="clpX"
                     /locus_tag="VW049_000037"
     CDS             34041..35273
                     /gene="clpX"
                     /locus_tag="VW049_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9431242.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            35282..35869
                     /locus_tag="VW049_000038"
     CDS             35282..35869
                     /locus_tag="VW049_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9431243.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            35880..36260
                     /locus_tag="VW049_000039"
     CDS             35880..36260
                     /locus_tag="VW049_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9431244.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            36312..37202
                     /gene="rapZ"
                     /locus_tag="VW049_000040"
     CDS             36312..37202
                     /gene="rapZ"
                     /locus_tag="VW049_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9431245.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAAAGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVIDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            37199..38176
                     /locus_tag="VW049_000041"
     CDS             37199..38176
                     /locus_tag="VW049_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9431246.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDFRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            38173..39084
                     /gene="whiA"
                     /locus_tag="VW049_000042"
     CDS             38173..39084
                     /gene="whiA"
                     /locus_tag="VW049_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9431247.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(39118..40086)
                     /locus_tag="VW049_000043"
     CDS             complement(39118..40086)
                     /locus_tag="VW049_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9431248.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIYPEQKVQPKHSTSL"
     gene            40222..41079
                     /locus_tag="VW049_000044"
     CDS             40222..41079
                     /locus_tag="VW049_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9431249.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYMSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            41066..41845
                     /locus_tag="VW049_000045"
     CDS             41066..41845
                     /locus_tag="VW049_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9431250.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            41869..43221
                     /locus_tag="VW049_000046"
     CDS             41869..43221
                     /locus_tag="VW049_000046"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9431251.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            43295..43669
                     /locus_tag="VW049_000047"
     CDS             43295..43669
                     /locus_tag="VW049_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9431252.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            43671..44519
                     /locus_tag="VW049_000048"
     CDS             43671..44519
                     /locus_tag="VW049_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9431253.1"
                     /translation="MKLAVFTDSSAYLSAETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            44785..45552
                     /locus_tag="VW049_000049"
     CDS             44785..45552
                     /locus_tag="VW049_000049"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9431254.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            45564..46748
                     /locus_tag="VW049_000050"
     CDS             45564..46748
                     /locus_tag="VW049_000050"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9431255.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYIPDMASSQNKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQARGKEVNPQAITEKYQ
                     SLTIHDKKDIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            46745..48616
                     /locus_tag="VW049_000051"
     CDS             46745..48616
                     /locus_tag="VW049_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9431256.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYRGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIEALEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(48759..49943)
                     /gene="mntE"
                     /locus_tag="VW049_000052"
     CDS             complement(48759..49943)
                     /gene="mntE"
                     /locus_tag="VW049_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9431257.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLENRF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            50247..52943
                     /locus_tag="VW049_000053"
     CDS             50247..52943
                     /locus_tag="VW049_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9431258.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDITPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            52956..53747
                     /gene="yghU"
                     /locus_tag="VW049_000054"
     CDS             52956..53747
                     /gene="yghU"
                     /locus_tag="VW049_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9431259.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLNLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            53863..54273
                     /locus_tag="VW049_000055"
     CDS             53863..54273
                     /locus_tag="VW049_000055"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9431260.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            54285..55880
                     /locus_tag="VW049_000056"
     CDS             54285..55880
                     /locus_tag="VW049_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431261.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVNDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            56047..56211
                     /locus_tag="VW049_000057"
     CDS             56047..56211
                     /locus_tag="VW049_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9431262.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGIALV
                     LAAVWLGWPK"
     gene            56290..57018
                     /gene="yaaA"
                     /locus_tag="VW049_000058"
     CDS             56290..57018
                     /gene="yaaA"
                     /locus_tag="VW049_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9431263.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(57069..58553)
                     /locus_tag="VW049_000059"
     CDS             complement(57069..58553)
                     /locus_tag="VW049_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9431264.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILEGLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
BASE COUNT        17033 a        10994 c        13420 g        17195 t
ORIGIN      
        1 agtcaaatcg atttctaaaa atgttttaga agtagaggtg tactattcta gttccaatct
       61 actatataaa tgaaaacctt agctgtttgg ccaaggttca tttcttctat gcaagttctt
      121 ctcttttctt atctgggttc cagatgaagc ttgcgataaa gctaaagatg atggttagga
      181 tagcgaggat aatagcaagg atgagggcag ggatgcggat aaaccaccag agtggatttg
      241 gttttttatc attgtgccat tgtttcgttt cttcgtccag acgttggaat tctgtttgaa
      301 tacgattggc gactgtttca atgccttcat cattcatttt cttgatatct gagatatcga
      361 ttggatttcc aaagttcata tcgacacgtt cacggctaat caagcccttc aaagtcatgg
      421 gaccggtgta ggtaaccggc atgatacgga ccttggccat tttggcaatc agtgctgcgc
      481 cccccttgac atcgtttgag tggcggctac cacttggaaa catgatgaga gagcggtcac
      541 tttttttgag aacgttgata ggatatttga tggctgaggc gctgggattt tcacggtcga
      601 tgggaaaggc gccacacata cgaatccacc aaccaaagat acggttggta aagagttctt
      661 tttttgccat aaagatgaac tgttttggct tggtcgcaaa ggccatataa acaggatccc
      721 accaggtacg gtgaggcgca actaaaatat aattttcatc ttgattagga attttatcag
      781 tattatgata gtgagcattg ccattgatgg accataagag caatacaact aatccacgca
      841 aataagtata aaacatgcga tctccttcga ttgttttctt gttattatta taccttatca
      901 aaggagggct ggcaaacttt tcccttgact agatacatat ttaggatgaa attagaattc
      961 tgttaaaaaa aatgatataa tagaatttat ggataaaaat aagattatgg gattaaccca
     1021 aagagaagtc aaggaaagac aggctgaggg tttggtcaat gactttaccg catcagccag
     1081 taccagcact tggcaaatcg ttaaacgaaa tgtctttacc ctttttaacg ctttgaactt
     1141 tgccattgct ttggctcttg cctttgtgca ggcttggagc aatctggtct tctttgctgt
     1201 tatctgcttt aacgcttttt ctgggattgt gaccgagcta cgagccaaac acatggtgga
     1261 caagctcaat ctcatgacca aggaaaaggt caaaaccatc cgtgatggtc aggaagttgc
     1321 tcttaatcct gaagaattag tgctaggaga tgtcattcgt ttgtctgcag gagagcagat
     1381 tcctagtgat gccttggttt tggaaggctt tgcggaagtc aatgaagcca tgttaacggg
     1441 agaaagtgat ttggtgcaaa aggaagttga cggcttactt ttgtcaggaa gtttcctagc
     1501 cagtgggtca gttttatctc aagttcacca tgtcggagca gacaactatg ctgccaaact
     1561 catgcttgag gctaagaccg ttaaacccat caactcccgt atcatgaaat cgctggacaa
     1621 gttggctggt tttactggga agattatcat tccctttggt ctggctctct tgctggaagc
     1681 cttgctttta aaaggcctgc ctctcaagtc atccgttgta aactcgtcga cagctctttt
     1741 gggaatgttg cctaagggaa ttgccctttt gaccattact tcgcttttga ctgcagtgat
     1801 taagctaggt ttgaaaaagg tcttggtgca ggagatgtac tctgttgaga ccttggcgcg
     1861 cgtggatatg ctctgtctgg acaagacggg caccatcacc caaggaaaga tgcaggtgga
     1921 ggctgttctt ccactgacgg aaacttacgg tgaagaggct attgccagca tcttgactag
     1981 ctacatggcc catagtgagg ataaaaatcc aacagctcaa gccattcgcc agagattcca
     2041 aggtcaagta gtctatccta tgctctctaa tcttcccttc tccagcgacc gcaagtgggg
     2101 agccatggag ttagaaggtt tgggaatagt tttcttaggg gcacctgaga tgttgctgga
     2161 ttctgaggtc ccagaagcca gagaggcctt ggagagaggt tcacgtgtct tggtcttagc
     2221 tctcagtcag gagaaattag accatcacaa accacagaaa ccatctgata ttcaggctct
     2281 agccttgctg gaaatcttgg atcctattcg agagggagcg gcagagacgc tgaactatct
     2341 ccgttctcag gaagtggggc tcaagattat ctctggtgac aatccagtta cggtatccag
     2401 cattgcccag aaggctggtt ttgcggacta tcacagctat gtcgattgtt caaaaatcac
     2461 ggatgaggaa ttgattgctc tggcggagga gacagcgatt ttcggacgtg tttcccctca
     2521 tcaaaagaaa ctcattatcc aaacgctgaa aaaagcgggt catacaacgg ctatgacagg
     2581 ggacggggtt aatgatatct tggcccttcg tgaggcggat tgttctatcg taatggcgga
     2641 gggagacccg gcaacccgtc agattgccaa cctggttctc ttgaactcag actttaatga
     2701 tgttcctgag attctttttg aaggtcgtcg tgtggtcaat aacattgccc acatcgctcc
     2761 gattttcttg ataaagacca tctactcatt cttgctcgca gttatctgta tcgccagtgc
     2821 tttactaggt cggtctgaat ggatcttgat tttccccttc attccaatcc agattaccat
     2881 gattgaccag tttgtggaag gtttcccacc attcgttctg acttttgagc gaaatatcaa
     2941 acctgttgag ccaaatttcc tcagaaaatc catgcttcgt gccctaccaa gcgctctcat
     3001 ggtcgtgttt agcgtcctgt ttgtgaaaat atttgggagt agccaaggtt ggtctgagtt
     3061 agaaatctca actctcctct attatctctt ggggtcaatt ggtttcttat ccgtatttag
     3121 agcctgcatg ccatttaccc tatggcgagt cctattgatt gtctggtcag taggaggctt
     3181 cctagccaca gctctcttcc caagaattca aaaactgctt gaaattgcaa ccttaacagg
     3241 acaaacatta cctgtttatg gtgtcatgat gttggtcttt accgtgattt tcatcctgac
     3301 cagtcgctat caagccagaa aataaagaaa ggctgcaatc tgtggattgc agttttttta
     3361 ggtacaagat tgccagtcga aatgtggtat aataagaggt aatagagttt tggaaagtga
     3421 gagaagatga tttcaaagag attagaattg gtagcttcct ttgtgtcaca gggggctatt
     3481 ttactagatg tgggaagtga ccatgcttat ctgcctatcg agttggttga gagaggccaa
     3541 atcaaaagcg ctattgcagg tgaggtggtg gaaggtccct atcagtctgc ggttaaaaat
     3601 gttgaggctc acggcctaaa ggagaaaatc caagtccgtt tagccaatgg cttggcagct
     3661 tttgaagaga ctgaccaagt gtctgtcatt accattgctg gcatgggtgg tcgtttgatt
     3721 gctaggattt tagaagaagg tttggggaag ttagctaatg tagagcgttt gatcctccag
     3781 cccaataatc gtgaagacga cttgcgtatc tggctacagg atcatggatt ccagattgta
     3841 gcagaaagca tcttagaaga agctggaaag ttttatgaga ttttggtggt ggaagcagga
     3901 caaatgaagc tatcagccag tgatgttcgc tttggtccct tcttgtccaa agaagtcagt
     3961 ccagtatttg tccaaaaatg gcaaaaagaa gctgagaagc tagagttcgc cctcggacaa
     4021 atcccagaaa aaaatctgga agaacgtcaa gttctagtag ataagattca agctatcaag
     4081 gaggtgctcc atgttagcaa gtgaagtgat ccaagcttat gaagcctttt gccctcagga
     4141 attttctatg gagggagaca gccgtggtct gcaaattggc actttagaca agggtatcca
     4201 aagggtcatg gttgccctcg atattcgtga agagacagtg gcagaggcca ttgaaaaggg
     4261 tgtggacttg attatcgtca agcacgcgcc tatctttcgt cctatcaagg acttgcttgc
     4321 cagccgtccg caaaatcaga tttacatcga cctgattaag catgatatcg cagtttatgt
     4381 cagccatacc aatattgata tcgttgaaaa tgggctcaat gactggtttt gtcagatgct
     4441 aggaatcgag gagacgactt atctgcagga aacaggtcca gaacgtggaa ttggacgtat
     4501 tgggaatatt cagcctcaga cattttggga attggcccaa caggtcaagc aagtctttga
     4561 cctagatagc cttcgaatgg tgcattatca agaggatgat ttgcagaagc ctatttcaag
     4621 agtagcaatt tgtggtggaa gcggacagtc tttctataag gatgctttgg caaagggggc
     4681 agatgtctat atcactggtg atatctacta ccatactgct caggatatgc tgtctgatgg
     4741 cttgttagca ttggatccag gtcactatat cgaagtgctt tttgtggaaa aaatcgcaac
     4801 actccttact caatggaaag agaaaaaagg ctgggaacta gagattttac ctagtcaagc
     4861 atcgaccaat cctttccgtc atatctagtt agaaagtgaa gacaatgaaa aaagttgcca
     4921 ttattggagc aggaattgtg ggagcaacag ctgcctacta cctctcgaga gaaagtgacc
     4981 tagaggtgac cgtttttgac catgagcaag gtcaagccac caaggctgca gcaggaatta
     5041 tcagtccttg gttttccaaa cgccgtaata aagcctggta caagatggcg cgcttggggg
     5101 ctgattttta tgtggattta ttagctgatt tagagaaatc aggacaaaaa atcgactttt
     5161 accagcgttc gggagtcttt ctcttgaaaa aggatgaatc caagttggaa gaactttatc
     5221 aactggccct ccagcgcaga gaagaatctc ccttgatagg gcaattagcc attctggacc
     5281 aagcctcagc taatgaatta ttccctggtt tgcagggatt tgaccgcctg ctctatgctt
     5341 ctggtggagc gagagtagat ggccacctct tagtgactcg tttgctggaa gccagtcagg
     5401 tcaagctggt caaagaaaaa gtgactctga caccgttagc atcaggctac cagattggtg
     5461 aagaggagtt tgagcaggtt attttggcga cgggagcttg gttgggggac atgttagaac
     5521 ccttgggtta tgaagtagat gttcgtcctc aaaaaggaca actacgagat tatcagctgg
     5581 tccaagacat ggaagcttac cctgttgtca tgccagaagg ggagtgggat ttgattcctt
     5641 ttgcaggtgg gaaattgtcc ttaggcgcta cccacgaaaa tgacatggga tttgatttga
     5701 cggtagatga aaccttgctc caacaaatgg aggaggccac cttgactcac tatctgattt
     5761 tggctgaagc tacttcaaaa tctgagcgtg tgggaatccg tgcctatacc agtgatttct
     5821 ctcccttctt tgggcaggtg ccagaattgg caggtgtcta tgcagccagt ggactaggtt
     5881 catcaggcct cacaactggt cctatcattg gttaccatct agcccaactg atccaagaca
     5941 aggagttgac cttggaccct ctaaattacc caattgaaaa ctatgtcaaa cgagtaaaaa
     6001 gcgaataata ctcaatgaaa atcaaagaac aaactaggta actagccgcg ggttactcaa
     6061 agcactgctt tgaggttgtg gatagaactg acgaagtcag ctcaaaacac tgttttgagg
     6121 ttgtggatag aactgacgaa gtcagtaacc atacccatgg caaggcgacg ttgacgtggt
     6181 ttgaattgga ttttcgaaga gtataagcat tttactgaaa ttttatcagg tagtttagga
     6241 tggcaaatga cattccctgt caaaaatggt aaaataagaa aaaataatct gagaatcgag
     6301 gaaaaaagat gcaagaaaag attttggtaa ctggtggtgc cggttttatt ggaacccaca
     6361 ctgttattga attgatccaa gcaggccatc aagttgttgt ggtggataat cttgtcaata
     6421 gcaatcgtaa gagtttagaa gttgttgaaa gaatcacagg agttgagatt cctttctatg
     6481 aggcagatat tcgtgacaca gctaccctca gagatatttt caagcaggaa gaaccgactg
     6541 gtgtcattca ctttgctggt ttgaaggctg ttggcgaatc aacacgtatc cctcttgcct
     6601 actatgacaa caatatcgct ggaactgtca gccttttgaa agccatggaa gaaaacaact
     6661 gtaaaaacat catcttcagt tcttctgcga cagtttacgg ggatccgcac acagtgccca
     6721 tcttggaaga tttcccactt tcagtgacca acccatacgg tcgtactaag ctcatgctag
     6781 aggaaatttt gactgatatt tacaaagcag actcagaatg gaatgttgtc ttgcttcgtt
     6841 actttaaccc aatcggagcc catgagagtg gtgatttggg agaaaatcca aacggtattc
     6901 caaacaatct cttgccatat gtgactcaag tagccgttgg aaaattagag caagtgcaag
     6961 tgtttggaga cgattacgat acggaagatg gaacaggtgt tcgtgactat atccacgttg
     7021 tcgatttggc taagggtcac gttgcagctt tgaaaaaaat ccaaaaaggt tcaggactaa
     7081 acgtttataa ccttggaact ggtaaaggtt actcagttct tgaaattatc caaaacatgg
     7141 aaaaagcggt gggacgtcct attccttacc gcatcgtaga acgtcgccca ggtgatatcg
     7201 ctgcctgcta ctcagaccca gcaaaagcta aagcagaact cggttgggaa gcagaactcg
     7261 acatcaccca aatgtgtgaa gacgcatggc gttggcaaag caagcatcca aatggatttg
     7321 aagactaaga tgatgatttc aatcatcgtc ccttgtttaa acgaagagga agtacttcct
     7381 cttttttatc aggctctgga agctttactt ccagatttgg aaacagaaat cgagtatgtc
     7441 tttgtcgatg atggatcaag tgatgggacc ttggaactct taaaggccta tcgggagcaa
     7501 aatccggcag tccattatat ttctttctct cgaaattttg gcaaagaagc agccctttat
     7561 gcaggcttgc aatatgcgac aggagatttg gtggtggtga tggatgcaga cctccaagat
     7621 cctcctagta tgttgtttga gatgaaaaat gtactagaca aaaatgtaga cttggactgc
     7681 gttgggacac ggagaactag tcgggaggga gaacccttct ttcgcagttt ctgtgctgtt
     7741 ctcttttatc gcctcatgca aaaaatcagc ccagtagctc tgccgtcggg tgtccgtgat
     7801 tttcgtatga tgagaaggtc tgtagtcgat gccattttaa gcttgaccga gtccaatcgt
     7861 ttttcaaagg ggctcttcgc ctgggtgggt ttcaaaacct actatttgga ctatccaaat
     7921 gtcgaaaggc aggctggcaa gaccagttgg agttttaggc aactcttttt ttactccatt
     7981 gaagggattg ttaatttttc agatttccct ttgactatag cctttgtagc tggtctccta
     8041 tcttgttttc tttctctgct gatgaccttt tttgttgtgg ttcggaccct cattttgggc
     8101 aatccgacat ctggttggac ctctctgatg gctgttattc tctttcttgg aggcattcaa
     8161 ctcttgacca ttgggattct cggtaagtat atcagtaaga tttatttaga gactaaaaaa
     8221 agaccacttt atcttatcaa agaaaaaagt gaccttcctg attttacaga aaaaataaag
     8281 tgaaaagact ataattttac atggaaatgt gctaaactag agggagtgga ttatcgccat
     8341 tgatttaggc ttcctggttt ccaatcaggg cgcgagttgg gcaattttta acagcctcta
     8401 agacatcctg actaggagaa atttcttttt ccagttggtc agggtcatcg taaaaacgca
     8461 cgattccatt atcgtggtaa tcaaataaat cagaataagt ttggcaaagt ccacaggcga
     8521 tacatcgttc aggtataagt gtgattttca tgttcatatt tatattgtaa taagaaaagt
     8581 aaaaaaaaac aaggagtaag gtatggcaaa agaaccgtgg caagaagata tttatgatca
     8641 agaagaatca agagcagggc atcggcatcg aaaccacgga ggggctgata ggatggctaa
     8701 tcgtattttg acgatcctag ctagtatttt ctttgtaatt gtggtggtga tggtcatcgt
     8761 tctcatctat ctatcatcgg gggggagtaa tcgcacagca gccttaaaag actttcatga
     8821 ttctgatgca agtgtagtac aaatctcatc ttcaagcagt tctcagcctg agcagagttc
     8881 agagccagaa tctacttcta gtagttcaga agaagctgct aatcctgaag gaacgattaa
     8941 agttctcgca ggagaagggg aagcagctat tgccgctcgt gcaggaatct ccattgctca
     9001 gttagaggcc ttgaatcctg ggcacatggc tacaggatct tggtttgcta atccaggtga
     9061 tgttataaaa ataaaatagg agccgatatg aaaacaattc aaattgctat tgatggtcct
     9121 gcttccagcg gtaagagtac ggtcgcaaag attattgcta aggattttgg gttcacctac
     9181 cttgatacag gagctatgta tcgtgcagcg acctatatgg ctcttaagaa ccaattagga
     9241 gttgaagaag tcgaagccct tctagccttg ttggaccagc atccaatcag ctttggacgt
     9301 tcagaaactg gagaccagct tgtttttgta ggagatgtgg atattaccca tcctatccgt
     9361 gaaaatgaag tgaccaatca tgtttctgct attgcagcaa ttcctgaagt gcgtgagaaa
     9421 ctggtttctc tccaacaaga aattgcccag caaggcggga ttgtcatgga tggtcgcgat
     9481 attggaactg ttgtattgcc acaagcagaa ttgaaaattt tcctagtagc ttctgttgat
     9541 gagagagcag agcgtcgtta caaggaaaat attgccaagg gaattgaaac agaccttgaa
     9601 accctaaaaa aggaaattgc tgcgcgtgac tacaaggata gtcatcgtga gacttctcct
     9661 ctcaaacaag cagaggatgc tgtctacctt gatacaactg gtttgaacat tcaagaagta
     9721 gttgaaaaaa tcaaagcaga agctgaaaaa agaatgtaaa tgtagaaaag ccgatagatt
     9781 gatatcggtt tttttctaaa tttgtcaaaa tgctgatttt agggtataat agttgctgtt
     9841 cgagaaattt tgattttcaa agaatagtga atgatgataa ggagaaacca tgaacaattt
     9901 accaaattgt ccaaaatgta actcagagta tgtctacgaa gacggtgctc tactggtttg
     9961 cccagagtgt gctcatgagt ggaatcctgc tgaagttgca gaagtagaag aaggtcttgt
    10021 cgctatcgat gccaacggaa ataaattgac tgatggtgat acagtaactc tcatcaagga
    10081 cttgaaagta aaaggtgcgc caaaagattt gaaacaaggg acgcgcgtga aaaatatccg
    10141 catcgtagaa ggcgatcaca atatcgactg taagattgat ggttttggtg ccatgaaact
    10201 taaatcagag tttgtgagga agatttaagc atgtcaaaac actataaact tgtattttat
    10261 agccgtatct tcttgtttct agcggctttt acgggagttt atcttgaaat tactaggcat
    10321 ggtggttttg ggatgcttct ctattacacg gttctgtcca acctcttggt aactattttt
    10381 accctttatc ttctaaaggt tatgagccgt gtaggtgaaa attggcaaag gccaagtctc
    10441 ttgcgcttaa aaggtggggt caccatgagt atcatgatta cctgtgtgat ttaccatttc
    10501 ctcttggcgc ccattgcgac taatttctat accctagaaa atttcctttg ccactatatc
    10561 gttcccatct ggtttttagc ggataccctc ttttttgaca aacagggtca atacaagatt
    10621 tgggatccag cagtgtggac cattttaccc tttctgtata tgatgtttgc tctttttaat
    10681 ggcttggttc taaaactcaa tattccaaat gccaaggata atcctttccc ttacttcttt
    10741 ttgaatgtga acaagggttg gaatgttgtg tttaagtggt gtctgattat ctttgttgcc
    10801 tatatggtag caggatttat tttctacttt atcaagcaaa tcaagagaaa gtcatcctaa
    10861 tactcttcga aaatctcttc aaaccacgtc agtttccatc tgcaacctca aaacactgtt
    10921 ttgaacaacc tgcggctagc ttcctagttt gctctttgat tttcattgag tataagatac
    10981 aagggattca ttttcgaatt taagaaggag tctacaaacc aatgaggctt cttcttttct
    11041 tgcttatttg ataactatca aaaaagtaga agattaagag aaatatagaa agagatttca
    11101 tgaaacaatt tttagaacgg gccagcattt tggctctctc cctcgttttg attacctcct
    11161 tttccatttc gagtgcccta ccagccatgt ttgactatta tcagggttat tctaaggaac
    11221 aaattgagct cttggtgagc ttgccttcct ttggaatcat gatgatgtta ctgctaaatg
    11281 gtttcttaga aaaaatattt cctgagcgct tacagattag tttgggcttg ctgattttat
    11341 cattgagcgg tacagctccc ttctggtacc aagcctatcc ctttgtcttt ggaacacggc
    11401 ttctctttgg tttgggtctt gggatgatca atgccaaggc catttctatt atcagtgaac
    11461 gctaccaagg aaaaaggcga attcagatgt tagggctacg cgcttctgca gaggtcgttg
    11521 gagcttctct cattaccttg gccgtcggtc agttgttggc ctttggttgg acagctatct
    11581 ttctagccta tagtgctgga tttttggtgc tgccccttta tctgctcttt gtcccttatg
    11641 gaaaatcaaa gaaagaagtc aagaaaagag cgaaggaagc aagtcgttta actcgagaaa
    11701 tgaaaggctt gatttttacc ttagctatcg aagcggcagt tgtagtttgt accaatacag
    11761 ctattaccat ccgtattcca agtttgatgg tggaaagagg attgggggat gcccagttat
    11821 ctagttttgt tcttagtatc atgcagttga tcgggattgt ggctggggtg agtttttctt
    11881 tcttgatttc tatctttaaa gagaaactgc tcctctggtc tggtattacc tttggcttgg
    11941 ggcaaattgt gattgccttg tcttcatcct tgtgggtggt agtagcagga agtgttctgg
    12001 ctggatttgc ctatagtgta gtcttgacga cggtctttca acttgtctct gaacgaattc
    12061 cagctaaact cctcaatcaa gcaacttcat ttgctgtatt aggctgtagt ttcggagcct
    12121 ttacgacccc atttgttcta ggtgcaattg gcttactaac tcacaatggg atgttggtct
    12181 ttagtatctt aggaggttgg ttgattgtaa tctctatctt tgtcatgtac ctacttcaga
    12241 agagagctta ggattgattc ctaagttttt cttttgatac tcaatgaaaa tcaaagagca
    12301 tagttgattg agtttggaat agtacgctgt agttgctaaa atatttctag aaattaattt
    12361 gacttttcta atagagttat tcatatctta tttcaatcta ctatagaaag ctagccgcag
    12421 gttgctcaaa atagtgtttt gaggttgcag atggaaactg acgtggtttg aagagatttt
    12481 cgaagagtat gagattttta tacccataca atatttgttt taggtccttg tttacagact
    12541 aatttcttga taaaatagag gttatgacga gatataaagc aactatttcc tatgatggtt
    12601 atgcctttgc tggctttcag cgccagtctc atgcgcgtag cgttcaggaa gaaattgaaa
    12661 aaaccttgac cagattaaat aaagggcaaa ccattactgt tcacggtgct ggtaggacag
    12721 atagtggggt tcatgccctg ggacaggtca ttcattttga cctgccttat cagatggatg
    12781 aggagaaact ccgttttgcc ttggataccc agtctcctga agatattgat gtgatttcga
    12841 ttgagcttgt ggcagatgat tttcattgcc gttatgccaa acatagcaag acctatgagt
    12901 ttattgtgga tagaggacgt cccaaaaatc ctatgcgccg tcactatgcc acccactttc
    12961 cctacccact cgatgtggaa cgaatgcaga ttgcaatcaa aaagctagag ggaacccatg
    13021 attttaccgg ttttacagcc tctgggacta gtgtagagga taaggttcgc accatcacag
    13081 aagctagttt aatagtcgat gagacaggac aatttttgac ctttaccttt tcaggaaatg
    13141 gtttcttgta taaacagatt cgcaatatgg tggggactct tctcaaaatc ggtaacaacc
    13201 gcatgccagt agagcagatt gatctcatct tggagaagaa ggacaggcaa cttgcaggtc
    13261 ccactgcagc accaaatggt ttgtatttaa aggagattcg ttatgaagaa taatcgtatt
    13321 ttagcacttt ctggaaatga tatttttagt ggtggtggac tgtcagctga tttggctacc
    13381 tataccttga acggcttgca tgggtttgta gcagtgactt gtttgacagc cttgacagaa
    13441 aaaggatttg aagtctttcc aactgatgat accatttttc aacatgaatt agatagcttg
    13501 cgtgatgtgg aatttggggg aattaagatt ggtcttctcc ctactgtcag tgtggctgag
    13561 aaggccttgg actttatcaa acaacgccca ggagtacctg tggtgttgga tcctgtcttg
    13621 gtctgcaagg aaacgcatga tgtagctgtc agtgagctct gccaagagtt gattcgcttt
    13681 ttcccttatg tcagtgtgat tacgcctaat ctcccagaag cagaattatt atccggtcag
    13741 gaaattaaaa ccttggaaga catgaaaact gcagcgcaga aattgcatga tttaggagcg
    13801 ccagcagtca ttatcaaggg aggcaatcgt cttagtcagg acaaggctgt ggatgtcttt
    13861 tatgatggac agacctttac tatcctagaa aatccagtta tccaaggcca aaatgctggt
    13921 gcaggttgta cctttgcctc tagcattgcc agtcacttga ttaaaggtga taaacttttg
    13981 ccagcagtag aaagctctaa ggctttcgtt tatcgtgcta ttgcacaagc agatcagtat
    14041 ggagtaagac aatatgaagc aaacaaaaac aactaaaatc gcccttgtat ccctattaac
    14101 cgccctttct gtggttctag gttatttctt aaaaatccca acacctacag gaattctaac
    14161 tcttttagat gctggtgtct tctttgcggc cttttacttt ggtagtcgtg aaggagcggt
    14221 agtcggagga ctagcaagtt tcttgattga cctcttatca ggctaccctc agtggatgtt
    14281 ctttagcttg gtcaaccatg gcttgcaggg atttttcgca ggatttaaag gaaaaagtca
    14341 gtggttaggc cttattttag caactattgc catggtagga ggctacgcct tgggttctac
    14401 tttgatgaat ggctgggcag cagccctccc agaaattcta ccaaatttca tgcaaaatat
    14461 ggtagggatg attgtaggat ttattcttag tcaaagtatc aagaagatta agtaatactc
    14521 ttcgaaaatc tcttcaaacc acgtcagttt ccatctgcaa cctcaaaaca ctgttttgag
    14581 cagcctgcgg ctagctttct agtctgattt tcattgagca taaagaggtt gagtaaaaag
    14641 tctttcaaat aagaaaaacg catagtatca ggtgttgaat gaacacctga tactatgcgt
    14701 tttattgtgg gaagattttc ctcattttct agtgaaattg aactttttct aacatctttt
    14761 gatagatgac ttcttaatca attatcattt ccattgttgt ttcttggggt ttcatccctt
    14821 gtctttgata aaagcggaga gcaccctcgt tatcattcca gacacttagg gttagattat
    14881 tgcagccaat ttctttagca taatagcaga aaaatgacaa taaaatagcc cctagatatt
    14941 ttgaaaggaa agcatagggg ctatatagtt taatcaaaat aaagcaaatc tttgctggtg
    15001 cttgtaaaga ggtcgaagcc atccttggta acaacaccgc agtcttcaat acgaacaccg
    15061 actttaccag ggatatagat acctggttca acagagaagc acatgccttc ttcgatgacc
    15121 atgtcgtttc cttccatgat agatgggaat tcatggacat ccataccgat accatgcccg
    15181 agacggtggt tgaagtactc accataacca gctttttcga tgacctcacg ggcagcgcgg
    15241 tccacttcat gagcagtcac acctggcttg ataaagtcaa gagcagcttg ttgggcttca
    15301 agagtcaagt tgtaaatatc tttcttgaat tggtctggtt tgccgacagc gactgtacga
    15361 gtcatatctg acgcatagcc attgaccaga acacccaggt caaagaggag aagagcatca
    15421 ttttcaacct tattagctgc tggaatgccg tgtggattcg cagcattatc accagtcaag
    15481 accatggtat caaagctcat ttcgtaacct tcacgtttca tggcaaagtc aatttgtgcg
    15541 atgatatctg tctcagtctt atcaagagaa atattgtcaa aaccaacatg aacagccttg
    15601 tcagcataaa gacctgcaac catcattttt tgcacttcat cagctgattt gatgaggcgc
    15661 atgcgttgga tacgaggagt gaggttgtca aactcagcag tctcaaaaac tgttttcaaa
    15721 ccatggtatt tggtcaagat gagattgtca aactcaacag cgacacgttt gaagtcaagt
    15781 tgtggaagag catgtttgat tttttgccat ggattttcag aatcgacata gcccactact
    15841 gggaaggaaa cggtgctact tgcacgttct acttcaagag ctgggacaaa gaggagaggt
    15901 tcctgatctg ctaggacaaa gaggaacatt tggcgttcat ggggatcact gtaaaaacca
    15961 gtgaggtaat tgattgtgac ggggtcagat acgacagcga cgtctagttt ttctgattca
    16021 agatatgtta ggatttgttg taatttagac atatgctacc ttctttctat ccctctattt
    16081 tggcaaaaaa tgagagaaaa tgcaagggag aagggtaaaa gaaaagacaa aaaactccga
    16141 caagataacg gagttctttg atatcatttt cctttgttta agaaaagtca gccttgcttt
    16201 tgacgtcact gtactcttca gcgatttctt ggctgaggat atcctcatag gcagggagtt
    16261 ggatatcctg accatatttc ttcttgaggg cagtagtaac taggcgataa gccagattgg
    16321 cattacgaga gaggataggc ccgtggaagt aggaaccaaa gacattctta taatgaaccc
    16381 cttcaccgac cttttcttcg ttgtttccat ttccatagac aacctgtccc agcggttttt
    16441 ggtcatcaga gaggaaggta cggccttggt gattttcaaa tccatagtag gtttcatcga
    16501 aatcttcatt gtgaatcttg atgtcaccga taaaacggtt attggtctgg ttgagcgtgt
    16561 agtgtcccat gacccctagc ccttcgatac gttttcctga agcttcaaca taatattgac
    16621 ccaatagttg gaaaccaccg cagatagcca gaactacacc gtcgttttgg atgtagttgt
    16681 caatgctctc ttttttagca ggtaggtcgt ctgcaatgat actttgttca aagtcttgac
    16741 caccaccgaa aaaggcgatg tcgtagtgat tttcatcaaa gtcatcatgg agagaaacga
    16801 tgtcaacggt cacatgggct cccagttttt cagccacata cttgagcatg aggatgtttc
    16861 cattgtcccc gtaggtattc atgagatttc cgtagaggtg ggcaatgttg agctgatagg
    16921 ggtaattgcc atcttttgag gaaagtgaag tataaaccat tagttcatct cctttctaac
    16981 aatctgacga ctagccagca gttcacgaaa ttccagcatg gcagtataag ttgccagaat
    17041 ataggcatgc ttgcagtctt gattctcaat ggtcttgaga acttgctcca gattactcgt
    17101 ttcagtgatt ttctcagctg gatagccagt cactcggagg cgacgagcga tttcagaatg
    17161 acgaacaccg ccagcgttga tttcaggaat gtccatgtca gtgatttgtt caaagtctgc
    17221 atcccagatc cagctagtgt caattccatc tgcatagttg gcattaagga ggacagatag
    17281 gctaaatgga taaggtgcta gtttgatcat ttcgatagct tgggttgcac cgactggatt
    17341 tttaatcaag acaagggtac attccttgtc accgatatga aaggtttctt ggcgtccaaa
    17401 gacagcacgg ctcttgtcaa atccctgttt gatgagttgc gaatcggcac ctaggaaacg
    17461 ggcgatggcc acagcagcta gggcgttata gatattatag agcccgccga tttggatacc
    17521 gtattcttgg ccgtctatga caaagcgaga gcgattgttg gtcaactcaa ccagttttgt
    17581 caaacgatag tcgagatcag gacgtttgca tccacaacct tcacagatat aggcacccaa
    17641 gtttgcatag gtattatgct catatttgag gatgccttgg cagtcaggac agagaatccc
    17701 ttcggtattg tagtgagcca gttgggctgg tcctttttcc aagtcaaaac caaaatactc
    17761 tatagggttt ggaatagttg gcttgtagaa aagtggactg tctccgttaa ggagaacagt
    17821 agcagttgga actttccgaa tggcttccaa tatcatgtta taggtagtat agatttcacc
    17881 gaaacggtcc atctggtcac ggaagatatt agtaatgaca aaaagactag gctggatata
    17941 gtcacagata cgagatagac tggcttcgtc aatttcgagg acggcaatat ttttcccagt
    18001 ttttgaagat ttggctgtta ggaaggttgt tgcaatccct gtaatcatgt tggcaccgct
    18061 tgggttggtt agaacttgac cataaacctc ttttaaaatg ccgacagtga gggcagttgt
    18121 cagggttttt ccatttgttc cagtgacaac gacaatctcg tagttcttag ctaggttttg
    18181 taaaatatct ttatcaaatt gaagggcgac tttacctggg agcgtacttc cacgtccaag
    18241 acggcttaaa acgaagtggg aagaacgccc agcaagaagg cccaaagtag tttttaagtt
    18301 catgtttcta ttatatcaca aaagagggaa aagacagatt tggtgtttcg tagaagtaaa
    18361 aacagcccaa agagggctgt cagttaagat gagatttcaa cttaaatcgc aaacaagtca
    18421 ttcaagttct cagccaaggt tgggtgagtg aagatttgtt ttgtgaagta agtgtaagga
    18481 atcttgttgt ccatagcaac agtaatgatg ttgatgattt cttgagaacc ttctgagaag
    18541 atgcttgcac caagaatttc ttttgtttca gtattaacaa cagctttgaa agttccgcga
    18601 aggtctccat ttacgtgacc acgaggcatg gctgcaacag ggatttcttt cactgcgtat
    18661 ggaagtttca aatcagctgc ttggctttca gtcaaaccaa cttgtgaaag tgcaggtgtg
    18721 atgaacatag tatttggcac attgagacgg tcctcaagtg tgtagctgcc atctccagca
    18781 aggtagctgt aaacaacacg gaagtcatca agtgaaatgt aagtaaattg aagaccaccg
    18841 ttgacatctc caactgcaaa gacaccagga acgtttgttt gacagtgttt atctacttta
    18901 atagcgccac gttcagttag ttcaatatct gtattttcaa gttgaagtgg ttctacattt
    18961 ggtttgcgtc cagttgcgta gagaagggcg tcgaaacggt aagtttcgtc ttcagttacg
    19021 acaagcactt ggtcaccgtc gtttttaatt tcagtagtat ggatattttg aagcaattca
    19081 ataccgtctt cttccaggta ttgtttagca agagctgcga tggaaggttc tgcacgaggt
    19141 aggaatgtat ccaaggcatc taggactgta accttgcttc ctagtttatt gtaaaggcca
    19201 gcaaattcaa gaccgatatt tccgccacca aggactccaa gtttttcagg caatttatcc
    19261 aagctttgga tacctgttga gtcaaagacg tttttgcttg tagcaagtcc agggattggc
    19321 aagacgtttg aaacagcacc agtgttgatg acgatagttt cagcagtcaa ttcttgtttt
    19381 tcatcaccag cttggatttc gatgacttta tttgaaagga agtgagcttc agcatcaaag
    19441 atatcgacgc cagtaccagc aacagtcgta tagtttttac cgttgaggcg accagtgatc
    19501 gtgtttttgg tagcaatgac ttcttcaaaa gacaagtctt tctcagcagc aaccagcaaa
    19561 gttttagttg ggatacaacc gatgttgata caagttccac cgtacatagc tttgctacgt
    19621 tcaacgaggg caactttttt gccagctgaa gccaatttac cagctagtgt tttaccagct
    19681 ttaccaaatc cgataacgat taaatcataa gttaacattt agaattcctc ctattcgaat
    19741 tacattctag cacaagaaaa aaacttttgc acaattctgc tacgaaaaag acagaaaaat
    19801 atgaaaacgc tttccaggca aaaaactagt aattttttga caagcttgat aaactaaaat
    19861 cctatctatt tttatataag atagaataaa tgttcgaaaa taggaggttt tatgaaatcg
    19921 aatcgttata ttattgcctt tgctggggtc attttacact taatgctggg ttcgacttat
    19981 gcctggagtg tttatcgtaa ccctatcatt gaaaaaacgg gatgggatca ggcttctgtt
    20041 gccttcgcct ttagtctagc aatcttttgt ttgggcttat cggctgcatt tatggggcgt
    20101 ttggtagaaa aatttggtcc gaaagtcatg ggaagtctat ctgcttttct atacgcaggt
    20161 ggaaatatct tagcaggatt tgcaatagac cgtcaggagc tgtggttgtt gtatctcgct
    20221 tatggcattt taggtgggct tggtttggga gcaggctata ttacccctgt gtcgacgatt
    20281 ataaaatggt ttcctgataa acgtggtctc gcaacaggtt tagcgattat ggggtttggt
    20341 tttgcttctt tattgactag tcccatagcg caacacctca tcgcaggggt agggcttgta
    20401 gaaacttttt atattttagg agcaagttac tttattatca tgctcctagc ttcacaattc
    20461 attaagcgtc caaatgagca agagcttgca attttatctt cttcagggaa agaaaaaaca
    20521 gcctctttga cgcaaggaat ggctgcaaat caggctctga aaagcaatcg gttttatatg
    20581 ctttggatta ttttctttat caacatagct tgtggtttag gcttaatttc agcggcatcg
    20641 ccaatggcac aggagatggc tggcttgtct acaagtcatg cagcagtaat ggtgggtgtt
    20701 ttggggattt tcaatggatt tggtcgcttg ctctgggcga gtttgtctga ctatatcggt
    20761 cgccctctaa cctttagtat attactgctt gtcaatcttt tcttttctct ctcactttgg
    20821 ctctttacag attccgtttt atttgtagtt gctatgtcta ttttgatgac ttgctatgga
    20881 gctggttttt ctttgattcc agcttatctc agtgatattt ttggaaccaa ggaattggcc
    20941 gctctgcatg gttatatttt aacagcttgg gcaatggctg gtttagcggg acctatttta
    21001 ttagcagaga cttataaaat ggctcattcg tacacacaaa ccttgttcgt ttttctcatt
    21061 ttatacagta tcgccttggc tttgtcttat tatctaggtc gttcaatcaa aaaagaaagt
    21121 caaaaaccgc ttacatgatt ttctgtagaa atggggctat cttttgctct ggtcttgtgt
    21181 tatactagat aggttgcaaa gaaaacagta cttttctttt gtggaaaaga agcaacacga
    21241 ttttatatct agttatatga aaatacgtca taaaaagaaa agtataaact aagccctata
    21301 gggtggcttc gcaccacctt tagaaagaag aataacgtga aatttaatga attaaacttg
    21361 tctgctgatt tgctagcaga aattgaaaaa gctggttttg tagaagctag tcctatccaa
    21421 gaacaaacta ttcctttggc ccttgaaggc aaggatgtta tcggtcaagc tcagactggt
    21481 acaggaaaaa ctgcagcctt tggcttgcct acccttgaaa aaatccgtac agaagaagcg
    21541 actatccaag ccttggtcat cgctccaact cgtgaactag ctgtccaaag tcaagaagaa
    21601 ctcttccgct ttggtcgtag taagggagtc aaagtccgtt cagtatatgg cggatcaagc
    21661 attgaaaaac aaattaaggc tcttaaatct ggtgcccata tcgtggtggg aactccaggt
    21721 cgcctcttgg acttgattaa acgcaaggcc ttgaaattac aagacattga aacccttatc
    21781 cttgacgaag cggatgaaat gcttaacatg ggcttccttg aagacatcga agccattatt
    21841 tcccgtgtac ctgagaaccg tcaaactttg cttttctcag caactatgcc agatgccatc
    21901 aaacgtatcg gtgttcagtt tatgaaagcc cctgaacatg tcaagattgc ggctaaggaa
    21961 ttgacaacag aattggttga ccagtactat atccgtgtta aggaacaaga aaaatttgac
    22021 accatgactc gtctcatgga tgtggcacaa ccagaactcg ctattgtatt tggtcgtacc
    22081 aaacgccgtg tggatgaatt gactcgtggt ttgaaaattc gtggcttccg tgcagaagga
    22141 attcatggcg acctagacca aaacaaacgt cttcgtgtcc ttcgtgactt taaaaatggc
    22201 aatcttgatg ttttggttgc gacagacgtt gcagcgcgtg gtttggatat ttcaggtgtg
    22261 acccatgtct acaactacga tattccacaa gatcctgaga gttatgttca ccgtatcggt
    22321 cgtacaggtc gtgctggtaa gtcgggtcaa tctattactt ttgttgctcc aaacgaaatg
    22381 ggttaccttc aaatcattga aaacttgact aagaaacgca tgaaaggtct caaacctgca
    22441 agtgtagaag aatccttcca atcaaaaaaa caggtagctc tcaagaaaat cgaacgtgat
    22501 tttgcagatg aaaccattcg tgccaacttt gagaaatttg gtaaggatgc tcgcaaattg
    22561 gctgctgagt ttactccaga agaattggca atgtatatct tgagtctgac agtccaagac
    22621 ccagatagcc ttccagaagt ggagattgca cgtgaaaaac cactaccgtt taaaccatca
    22681 ggtaatggtt tcggtggtaa agctaaggga ggtcgtggag gccgtcgtgg ggacgaccgt
    22741 cgagagcgtg atcgccgtgg caatggtcgc cgtgatgagt tcaaaaaagg aagtcgtggc
    22801 aacgatcgtt ttgataagga aaaacgttac cgtaaggata ataaaaaacc acgcaatact
    22861 ttaagcgaaa agcaaacagg ctttgttatt cgtaacaaag gcgataaata agaaaaagcg
    22921 gttcaaaatg aatcgctttt ttatataagg agaccgaatg tgaaaagaca aataaaatta
    22981 ttatattatc ttttgtaatg ttatgataca caaaaatagg caacttgtca atagaaaagg
    23041 aacaatttaa ttaaaaatat tctggttttt aattaaattg taagtcaatg caagtcaata
    23101 aggaattttc agataattat tgaaaaagag acgtttttat gttataatga tagcgattaa
    23161 attcgaggtg aaaaatggca catttattag aaaaaactag aaaaattaca tcaattttga
    23221 aacgctcaga ggagcagttg caggatgagc tcccttacaa cgctattacg cgtcagttag
    23281 cggatattat tcattgcaat gcctgcatta tcaatagtaa gggacgtctg cttggctatt
    23341 ttatgcgtta taaaacaaat acagatcgcg tagagcaatt cttccaaact aagattttcc
    23401 cagatgacta cgttcaaggg gctaatatga tttacgaaac agaagcaaac ttgcctgttg
    23461 agcatgatat gagtattttc cctgttgaga gtagagatga ttttccagat ggcttgacga
    23521 ctattgcacc gattcatgta tcggggattc gccttggttc tttgattatt tggcgtaatg
    23581 ataaaaaatt cgaagatgag gacttggttc ttgttgagat tgccagtacc gttgttggga
    23641 ttcagcttct taacttccaa cgtgaagaag atgagaaaaa tattcgtcgt cgtactgctg
    23701 tcaccatggc ggtcaatacc ctttcttact ccgaactccg tgctgtttca gcaattttag
    23761 gggaattaaa tggaaatgaa gggaagttga ctgcgtcagt gattgcagat cgtatcggaa
    23821 tcactcgctc tgtgattgtc aatgctcttc gtaaacttga gtctgcgggg attattgaaa
    23881 gtcgctcact tggaatgaag ggaacctatc ttaaggtctt gatttcagat atttttgaag
    23941 aagtgaagaa aagagattac taatgacaaa ggctttaatt tcgattgatt atacagaaga
    24001 ttttgttgct gatagtggga agctgacagc aggtgctcca gctcaggcga tttcggatgc
    24061 cattagcaag gtaactagat tagcttttga acgaggagat tatatcttct ttactattga
    24121 tgctcatgaa gaaaacgatt gtttccatcc ggaaagtaag ttatttcctc ctcataatct
    24181 gattgggacg agtggacgga atttatatgg agatttgggg atcttttatc aagagcatgg
    24241 ttcagacagt cgtgtctttt ggatggataa acgccattac tcagcttttt cagggactga
    24301 cctagatatt cgtttgagag agcgtagagt gtctaccgtt atcttaacag gtgtcttgac
    24361 ggatatctgt gtcctacata cagctataga tgcctataat ctaggatatg acatcgagat
    24421 tgttaaacca gctgttgctt ccatctggcc tgaaaatcat caatttgccc taggtcattt
    24481 caaaaataca ctcggagcta agttagtaga tgaaaatcta aatgaacttt ctgagtaacc
    24541 tggttggtga aaatgaaaaa atttgaaaaa aagtgttgac aagcatccca aaagttgata
    24601 tactagtaaa gtaatcgacg cggggatggc ggaattggca gacgcgcagg actaaggatc
    24661 ctgtgaccgc tttaggtcgt gagggttcaa gtccctctct ccgcatagga aaagagttag
    24721 ctaaagctag ctcttttgta tttctctaaa aaattagaat ccagtcaaat gtagtggaga
    24781 atcagcgaaa atatgggaaa actgatcctt gtttctgctt ttgtcataga agaataataa
    24841 agattatctt cttcaaattc tccgatattc tctaaagttt tgtgcaagtt gcacagaact
    24901 tttttatttt tttggtcatc ttgccataga aatataaagc gttttcatct ataatataat
    24961 tatcaaaaga caaaaggagt tcacctcatg gtagaattga atcttaaaaa tatttacaaa
    25021 aaatatccaa acagcgaaca ctattcagtt gaagatttca acttgaacat caaagataaa
    25081 gaatttatcg ttttcgtagg accttcagga tgtggtaaat caactacact ccgtatgatt
    25141 gctggtcttg aagacattac agaaggtact gcatctatcg atggcgtggt tgtcaacgac
    25201 gtagctccaa aagaccgtga tatcgccatg gtattccaaa actacgctct ttacccacac
    25261 atgactgttt atgacaacat ggctttcggt ttgaaattgc gtaaatacag caaagaagac
    25321 attaacaaac gtgttcaaga agcagctgaa atacttggat tgaaagaatt cttggaacgt
    25381 aaaccagctg acctttcagg tggtcaacgt caacgtgttg ccatggggcg tgcgattgtc
    25441 cgtgatgcga aagtattctt gatggacgaa cctttgtcaa acttggatgc caaacttcgt
    25501 gtatcaatgc gtgctgaaat cgctaaaatt caccgtcgta tcggagctac aactatctat
    25561 gtaactcacg accaaacaga agcgatgaca cttgcagacc gtatcgttat tatgtcagct
    25621 actaagaacc ctgctggtac aggtactatc ggacgtgtag aacaaatcgg tactcctcaa
    25681 gaagtttaca aaaatccagt taacaaattc gttgcaggat tcatcggaag cccagctatg
    25741 aacttcatca acgtgaaatt ggttggtagc gaaattgttt ctgacggttt ccgtttgaaa
    25801 gtgccagaag gagcattgaa agttcttcgt gaaaaaggct acgaaggaaa agaattgatc
    25861 tttggtatcc gtccagaaga cgtgaatgca gaacctgctt tccttgaaac attcccagac
    25921 tgtgttgtaa aagcgactat ctctgtatca gaactgcttg gttcagaatc tcacctttac
    25981 tgccaagttg gtaaagacga gtttgttgca aaagttgatg ctcgtgacta cttgcaaaca
    26041 ggtgcaacag ttgagcttgg atttgacttg aacaaagcac acttcttcga tgtagaaact
    26101 gaaaaaacaa tctactaaaa taaataaaat tcaaagcact acaagaaaag atatctcttt
    26161 atcaattgta gtggagagat atcagttaat ctagggagag aaacaaaatg cttctctcct
    26221 ttttgctagg gaagtcatat tatgcatcta tattgtgatg ctctttaata ctcttcgaaa
    26281 atctcttcaa accacgtcaa cgtcgccttg ccgtacgtat gattactgat ttcgtcagtt
    26341 ttatctgcaa cctcaaagat gtactttgag cagcttacgg ctagtttcct agtttgctct
    26401 ttgatttcca ttgagtatta tttgtgggta ccatctacaa gtgaagctat atgcgtaaac
    26461 tacgtgagca attgaattcg aactagagag gtaataataa atttatgcta tagttatggt
    26521 gacttgtatg cttttgattc tagtttatca aataatagat tagaattgtc agataatatc
    26581 attttgtgtt ataatgaaga aaaaacagag gtgttcaaat gtcagaagca ggtcataagt
    26641 ttttagcaaa attggggaaa aaacgcttac gtccaggtgg aaagcgtgcc acagattggt
    26701 taattgcaga aggaggattt tcaaaagaaa agagaatact agaggttgcg tgtaataggg
    26761 gaactacagc aattgagttg gcacagcgtt ttggttgcaa gataactgct gttgatatgg
    26821 atgctcaagc tttagaagtg gctaaaaaat ctgctggaac ggcaggtgtt gctcatttaa
    26881 tcagttttga aagagcaaat gcaatgaaac ttccttatca agatgctagt tttgatattg
    26941 ttataaatga agctatgctg actatgcaag ccgatcaagc taagaaaaaa tgtgtaatgg
    27001 aatatctaag ggtattaaaa cctggaggtc ttctcttgac acatgatgtg cttcttaagg
    27061 aagctaaaga gtctatcaga caggaattat cacaagcaat tcatgtaaat gtaggtcctt
    27121 taactcaaga tggttgggaa caggtgatga tagaatcagg ttattgtgat gtgaaagcat
    27181 tgactggtga aatgacatta atgaaattat cgggtatgat ttatgacgaa ggtttgctag
    27241 gaactttgaa aatttgtgta aatgcttgta aaaaggagaa tagaaagcag tttttaacta
    27301 tgtataaaat gtttgctaag aataaacaga aattgggctt tattgcgatg gctagttata
    27361 aatcgtcaaa acgttagata attattgaag ttaacttttc cttttttctt tcttaaaaaa
    27421 tatgctataa tagagagtaa aaaactttga aagaaagaaa aaagatgaat ttaaaagatt
    27481 acattgcaac aattgaaaat tatccaaagg aaggcattac cttccgtgat attagtcctt
    27541 tgatggctga tggaaatgct tatagctacg ctgttcgtga aatcgttcag tatgctactg
    27601 acaagaaagt cgacatgatc gtgggacctg aagctcgtgg atttatcgtg ggttgtccag
    27661 ttgcctttga gttgggaatt ggttttgcgc ctgttcgtaa gccaggtaaa ttgccacgtg
    27721 aagttatttc tgctgactat gaaaaagagt acggtgtcga taccttgact atgcacgcgg
    27781 atgccattaa gccaggtcaa cgtgttctta ttgtagatga ccttttggcg acaggtggaa
    27841 ctgttaaggc aactatcgag atgattgaaa aacttggtgg tgttatggca ggttgtgcct
    27901 tccttgttga attggatgaa ttgaacggcc gtgaaaaaat tggtgactac gactacaaag
    27961 ttcttatgca ttattaatga aaacagtccc tagggctgtt ttctctacac tatgatataa
    28021 aaatagacta taactagtta gagaaaaact ataattgaaa actatatctt cttgcagtat
    28081 aataaaagga ctaagtgttt gagatttgtc ttcaaacata tgcaattatt cctgaaagag
    28141 tacagttagg agagggttat gccgattcga attgataaaa aattgccagc tgttgagatt
    28201 ttacggacag agaatatctt tgtcatggat gatcaacgtg ctgcccacca agatatccgt
    28261 cctttgaaga ttttaatttt aaatctcatg ccacagaaaa tggtcacaga gacccagttg
    28321 ttgcgccact tggctaatac acccctacaa ctggatattg attttctcta tatggagagc
    28381 caccgttcta aaacaactcg ttcagagcac atggagacct tctataaaac ttttcctgaa
    28441 gtcaaggatg agtattttga tgggatgatc atcacgggtg ctccagttga gcatttacca
    28501 tttgaggaag tggactattg ggaggaattt agacagatgc ttgagtggtc taagactcat
    28561 gtctattcga cccttcatat ctgttggggg gctcaggctg ggctttatct gcgctatggt
    28621 gtagaaaaat accagatgga cagtaagcta tcaggtattt atcctcagga caccctaaaa
    28681 gagggtcacc ttctatttag aggctttgat gatagctatg tatcccctca ttcacggcac
    28741 acggagattt ctaaggaaga ggtcttaaac aagaccaatc tcgagatttt atcagaagga
    28801 cctcaggttg gggtttctat tttggccagt cgtgatttac gagaaattta tagttttggt
    28861 catttggagt atgaccgtga taccttggca aaagagtatt ttcgagatcg tgatgcaggt
    28921 ttcgacccac atattccaga aaattacttt aaagatgatg atgttaatca ggtaccttgt
    28981 ctttgttggt cttcatctgc agccctcttt ttcagtaatt gggtaaacca tgcggtctat
    29041 caggagacgc cttttgattg gagaaagata gaagatgatg catctgcata tgggtattta
    29101 taagaggaat tatgacatat ttagacgctt ttaaatcagg taccttggtt ttaccgagtg
    29161 ccctgctctt gcattttaag gaactctttc cttctagcga cgattttctg gtttggcaat
    29221 ttttctattt gcaaaatacg acaggcttag aagaaatgtc gccaagccag attgctgaaa
    29281 ggattggcaa ggaaatttcg gatgtcaacc agtccatttc taatctgacg gaaaggggac
    29341 tgctccagta tcgtactatc gaattaaatg gcgaaattga attgctcttt gatgctagtt
    29401 tggccttgga acgtttggat gacctgtttg gagcagttca ttcaagttca gaccagctaa
    29461 catctcaaaa ccagctcaag gatttggtgg aaaccttcca gcaggagttg ggacgattgt
    29521 tgacgccttt tgagattgag gatttgacca agacactaaa ggaagatgga accagtgctg
    29581 acttgattaa ggaggctctt cgtgaagctg ttttgaatgg aaaaccaaac tggaagtaca
    29641 ttcaggcgat tttgagaaac tggcgccatg aaggaatcaa gagtgtggct caaattgagg
    29701 ccaagagagc agaaagagaa gcaagcaatc ctcagttgac acaggtatct gcagatttca
    29761 gaaatgccat ggatctctgg aaggattaat ccatgcaagt aggcttgaaa tccgagtaag
    29821 atttgcaagc tgtgtataat tgtgatagaa taaatagaaa ataaattgaa aaaagaggta
    29881 tgtgaaatgt cacgtaaacc atttatcgct ggtaactgga aaatgaacaa aaatccagaa
    29941 gaagctaaag cattcgttga agcagttgca tcaaaacttc cttcatcaga tcttgttgaa
    30001 gcaggtatcg ctgctccagc tcttgatttg acaactgttc ttgctgttgc aaaaggctca
    30061 aaccttaaag ttgctgctca aaactgctac tttgaaaatg caggtgcttt cactggtgaa
    30121 actagcccac aagttttgaa agaaatcggt actgactacg ttgttatcgg tcactcagaa
    30181 cgccgtgact acttccatga aactgacgaa gatatcaaca aaaaagcaaa agcaatcttt
    30241 gcgaacggta tgcttccaat catctgttgt ggtgaatcac ttgaaactta cgaagctggt
    30301 aaagctgctg aattcgtagg tgctcaagta tctgctgcat tggctggatt gactgctgaa
    30361 caagttgctg cctcagttat cgcttatgag ccaatctggg ctatcggtac tggtaaatca
    30421 gcttcacaag acgatgcaca aaaaatgtgt aaagttgttc gtgacgttgt agctgctgac
    30481 tttggtcaag aagtcgcaga caaagttcgt gttcaatacg gtggttctgt taaacctgaa
    30541 aatgttgctt catacatggc ttgcccagac gttgacggtg cccttgtagg tggtgcgtca
    30601 cttgaagctg aaagcttctt ggctttgctt gactttgtaa aataatcagt aagtagcaaa
    30661 agctaggtgg aacagcattc agatgtctgt tacatttttt ataggagagc aaagattgaa
    30721 aacaaaaatt ggattagcaa gtatctgttt actaggcttg gcaactagtc atgtcgctgc
    30781 aaatgaaact gaagtagcaa aaacttcgca ggatacaacg acagcttcaa gtagttcaga
    30841 gcaaaatcag tcttctaata aaacgcaaac gagcgcagaa gtacagacta atgctgctgc
    30901 ccactgggat ggggattatt atgtaaagga tgatggttct aaagctcaaa gtgaatggat
    30961 ttttgacaac tactataagg cttggtttta tattaattca gatggtcgtt actcgcagaa
    31021 tgaatggcat ggaaattact acctaaaatc aggtggatat atggcccaaa acgagtggat
    31081 ctatgacagt aattacaaga gttggtttta tctcaagtca gatggggctt atgctcatca
    31141 agaatggcaa ttgattggaa ataagtggta ctacttcaag aagtggggtt acatggctaa
    31201 aagccaatgg caaggaagtt atttcttgaa tggtcaagga gctatgatgc aaaatgaatg
    31261 gctctatgat ccagcctatt ctgcttattt ttatctaaaa tccgatggaa cttatgctaa
    31321 ccaagagtgg caaaaagtgg gcggcaaatg gtactatttc aagaagtggg gctatatggc
    31381 tcggaatgag tggcaaggca actactattt gactggaagt ggtgctatgg cgactgacga
    31441 agtgattatg gatggtgctc gctatatctt tgcggcctct ggtgagctca aagaaaagaa
    31501 agattttaat gtcggctggg ttcacagaga tggtaaacgc tatttcttta ataatagaga
    31561 agaacaagtg ggaaccgaac atgctaagaa aatcattgat attagtgagc acaatggtcg
    31621 tatcaatgat tggaaaaagg ttattgatga gaacgaggtg gatggtgtca ttgttcgtct
    31681 aggttatagc ggtaaagaag acaaggaatt ggcgcataac attaaggagt taaaccgtct
    31741 gggaattcct tatggtgtct atctctatac ctatgctgaa aatgagaccg atgctgagaa
    31801 tgacgctaaa cagaccattg aacttataaa gaaatacaat atgaacctgt cttaccctat
    31861 ctattatgat gttgagaact gggaatatgt aaataagagc aagagagctc caagtgatac
    31921 agacacttgg gttaaaatca tcaacaagta catggacacg atgaagcagg cgggttatca
    31981 aaatgtgtat gtctatagct atcgtagttt attacagacg cgtttaaaac acccagatat
    32041 tttaaaacat gtaaactggg tagcggctta tacgaatgct ttagaatggg aaaaccctta
    32101 ttattcagga gaaaaaggtt ggcaatatac ctcttctgaa tacatgaaag gaatccaagg
    32161 gcgcgtagat gtcagcgttt ggtattaagc gatgatttga aagagggatg tgatagtagc
    32221 accctctttt tctttgtttt atgatagttc atcctcgagt aaattcaaga tcttgctcgg
    32281 aaatgaagct tatatagtag attgaatata gacaaatacc ttgtgattgg taaaacattt
    32341 tagaaattca tttacctttc ctaatcgact tggtttcatc ttatttcaat ctattatagt
    32401 attggggaat ttcttcaaac cacatcagct tggtcagttc tacctgcgac ctcaaaactt
    32461 gtgctttggt caagctgggt ttagtttcct agtttgctga tggatttcca ttgactataa
    32521 gcatccaacc ctctttttgt cttctaaaga attcttaaat tatcagtcta ttgcaacttt
    32581 tctcatataa gttctttgtc ttgctattgg ttttccttag tagtatacta aggtagtaat
    32641 cattaagaag tggttacaaa aaataatgaa tgaggtaaaa taaaatggta gaattgaaaa
    32701 aagaagcagc aaaagatgtc gcaacattga caaaagcagc gccagtagca ttggcaaaaa
    32761 caaaggaagt cttgaaccaa gctgttgctg atttgtatgt agctcacgtt gctttgcacc
    32821 aagtgcactg gtatatgcat ggtcgtggtt tccttgtatg gcatccaaaa atggatgagt
    32881 acatggaagc tcttgacggt caattggatg aaatcagcga acgcttgatt acactcggtg
    32941 gaagcccatt ctctactttg acagagttcc ttcaaaacag tgaaatcgaa gaagaagctg
    33001 gtgaataccg taatgttgaa gaaagcttgg aacgtgttct agctatttat cgctacctca
    33061 tcactctttt ccaaaaagcg ttggatgtga ctgacgaaga aggcgatgat gttacaaacg
    33121 atatttttgt tggtgctaag gctgaacttg agaaaacagt ttggatgctt gccgcagaac
    33181 ttggacaagc acctggtttg taagaaacaa gacaacatag ataaatctgt aggaaatttc
    33241 ttacagattt tttctatttt gtaagctatt ccaaaccagt ctttttttga gttttttgat
    33301 aaaatagtac tatcagtgaa aaggatggaa gcatgactaa gaaaatcgta gctatttggg
    33361 cccaggatga agagggtgtg attggtaagg acaatcgtct gccttggcat ttgccagcag
    33421 aattgcagca ctttaaagaa acaactctga atcatgctat cttgatgggg cgggtaacct
    33481 ttgatggaat ggggcgtcgc ttgcttccaa aacgggagac cctgattttg acgcgtaatc
    33541 cggaagaaaa gatagatggg gttgctactt ttcaggacgt ccagtctgtt cttgactggt
    33601 atcaggctca agaaaagaat ctctacatta tcggtgggaa gcaaattttt caggcttttg
    33661 aagcttacct tgatgaagtg attgtcactc acattcatgc tcgggtggaa ggagatacct
    33721 atttccctga agagcttgac ttgtctcttt ttgagacagt ttcaagcaaa ttttacgcca
    33781 aagatgagaa gaatccttat gattttacca tccaataccg caagagaaag gaagtttaat
    33841 ggaacggagt ttatttggtc tattcacggc ttttctgtgt ttcatctgct ttctggcagg
    33901 agcacaagct tttcgtaaaa agcgttacgg gctttctatc ctcctttggt taaatgcctt
    33961 taccaatctg gtcaatagta ttcatgcttt ttacatgacc ttattttaga tagaatgaca
    34021 gtatagaatg gaaggaaatc atgtctacaa atagaaaaaa tgatatgatg gtttattgct
    34081 cattttgtgg caaaaaccaa gaagaagtac aaaaaataat tgctggcaac aatgctttta
    34141 tttgtaatga atgcgtggag ttagctcagg aaatcattcg agaagaattg gttgaggaag
    34201 tcttggcaga cttgtctgag gtgccaaaac caattgaact cctccatatc ttgaaccact
    34261 atgtaattgg tcaagatcgt gccaagcgtg ccttggcagt ggcggtttat aaccactaca
    34321 aacgcatcaa tttccacgat acacgcgaag agtcagaaga tgtggatttg cagaagtcaa
    34381 acattttgat gattggccca actggttcag ggaaaacttt ccttgcccag accttggcta
    34441 agagcttgaa tgtacctttt gctattgcgg atgcgacagc tctgacggag gctggttatg
    34501 tgggtgagga tgtggaaaat atactcctca aactcttgca ggttgctgac tttaacatcg
    34561 aacgtgcaga gcgtggcatt atctatgtgg atgaaattga caagattgcc aagaagagtg
    34621 agaatgtgtc tatcacacgt gatgtttctg gtgaaggggt gcaacaagcc cttctcaaga
    34681 ttatcgaggg aactgttgct agcgtaccgc ctcaaggtgg acgcaaacat ccacaacaag
    34741 agatgattca agtggataca aaaaatatcc tcttcatcgt gggtggtgct tttgatggta
    34801 ttgaagaaat tgtcaaacaa cgtctgggtg aaaaagtcat cggatttggt caaaacaata
    34861 aggcgattga cgaaaacagc tcatacatgc aagaaatcat cgctgaagac attcaaaaat
    34921 ttggtattat ccctgagttg attggacgct tgcctgtttt tgcggctctt gagcaattga
    34981 ccgttgatga cttggttcgc atcttgaaag agccaagaaa tgccttggtg aaacaatacc
    35041 aaactttgct ttcttatgat gatgttgagt tggaatttga cgacgaagcc cttcaagaga
    35101 ttgctaataa agcaatcgaa cggaagacag gggcgcgtgg acttcgctcc attatcgaag
    35161 aaaccatgct agatgttatg tttgaggtgc cgagtcagga aaatgtgaaa ttggttcgca
    35221 tcactaaaga aactgtcgat ggaacggata aaccgatcct agaaacagcc tagaggtgac
    35281 tatggaactt aatacacaca atgctgaaat cttgctcagt gcagctaata agtcccacta
    35341 tccgcaggat gaactgccag agattgccct agcagggcgt tcaaatgttg gtaaatccag
    35401 ctttatcaac actatgttga accgtaagaa tctcgcccgt acatcaggaa aacctggtaa
    35461 aacccagctc ctgaactttt ttaacattga tgacaagatg cgctttgtgg atgtgcctgg
    35521 ttatggctat gctcgtgttt ctaaaaagga acgtgaaaag tgggggtgca tgattgagga
    35581 gtacttaacg actcgggaaa atctccgtgc ggttgtcagt ctagttgacc ttcgtcatga
    35641 cccgtcagca gatgatgtgc agatgtacga atttctcaag tattatgaga ttccagtcat
    35701 cattgtggcg accaaggcgg acaagattcc tcgtggtaaa tggaacaagc atgaatcagc
    35761 aatcaaaaag aaattaaact ttgacccgag tgacgatttc atcctctttt catctgtcag
    35821 caaggcaggg atggatgagg cttgggatgc aatcttagaa aaattgtgag gaaaagaaaa
    35881 tggcaaaaac aattcataca gataaggccc caaaggctat cggaccctat gttcaaggaa
    35941 aaatcgttgg caaccttttg tttgctagcg gtcaagttcc cctatcccct gaaactgggg
    36001 aaattgtagg agagaatatc caagaacaga cagagcaagt cttgaaaaac atcggtgcta
    36061 ttttggcaga agcaggaaca gactttgacc atgttgtcaa aacaacttgt ttcttgagcg
    36121 atatgaacga ctttgttcct tttaatgagg tttaccaaac ggccttcaaa gaggaattcc
    36181 cagctcgttc agctgtggag gtagctcgtc ttcctcgtga tgtaaaagtc gaaattgaag
    36241 tcatcgcaga gattggataa gctagttgaa gtttggtgtt gccaaacttc ttttgatata
    36301 aggagaaaaa gatgacaaag aaacaacttc acttggtgat tgtgacaggg atgagtggcg
    36361 cagggaaaac tgtagccatt cagtccttcg aggatctagg ttatttcacc attgataata
    36421 tgccgccagc tctcttgcct aagtttttgc agctggttga aattaaggaa gacaatccta
    36481 agttggcctt ggtagtggat atgcgtagcc gttctttctt ttcagagatt caagctgttt
    36541 tggatgagtt ggaaaatcaa gatggtttgg atttcaaaat cctctttttg gatgcggctg
    36601 ataaggaatt ggtcgctcgt tacaaggaaa ccagacggag tcacccacta gcagcagccg
    36661 gtcgtatttt agatggaatc aagttggaac gtgaactctt ggcacctttg aaaaatatga
    36721 gccaaaatgt ggtggatacg actgaactca ctccacgtga gctgcgcaaa acccttgcag
    36781 agcagttttc agaccaagaa caagcccagt ctttccgtat cgaagtcatg tctttcggat
    36841 ttaagtatgg aatcccgatt gatgcggact tggtcattga tgtccgtttc ttgccaaatc
    36901 cctattattt accagaactg agaaaccaaa cgggtgtgga tgaacctgtt tatgattatg
    36961 tcatgaacca tcctgagtca gaagactttt atcaacattt attggccttg attgagccga
    37021 ttctgccaag ttaccaaaag gaaggtaagt ccgttttgac cattgccatg ggatgtacgg
    37081 gtggacaaca ccgtagtgtg gcatttgcta aacgcttggt gcaggactta tccaagaatt
    37141 ggtctgttaa tgaagggcat cgcgacaaag accgcagaaa ggaaacggta aaccgttcat
    37201 gagaaaacca aagataacgg tgattggtgg agggactgga attcccgtca ttctaaaaag
    37261 tctgcgggaa aaagatgtgg aaatcgcagc tatcgtgacg gtggcagatg atggtggttc
    37321 ttcaggtgaa ctccgaaaaa atatgcaaca gttgacaccg ccaggtgatt ttcgtaatgt
    37381 ccttgtggcc atgtcggata tgcctaagtt ttatgagaag gtctttcagt atcggttctc
    37441 tgaggatgcc ggagcctttg ctggccatcc attgggaaat ctcatcattg ctggcttgtc
    37501 agaaatgcag ggttcaacct ataatgccat gcagttattg agcaaatttt tccatacaac
    37561 agggaaaatt tatccttcca gtgaccatcc tttgaccctt catgcagtct ttcaggatgg
    37621 gacagaagtg gctggagaga gtcatattgt agaccatcga ggcataattg acaatgtcta
    37681 tgtgaccaat gccctaaacg atgatacgcc tctggccagc cgtcgagtag tgcagaccat
    37741 ccttgaaagt gacatgattg tcctagggcc aggttccctc tttacctcta ttttgcccaa
    37801 tatcgtgatt aaggaaattg ggcgggctct tttggaaacc aaggcagaaa ttgcctatgt
    37861 ctgcaatatc atgacccaac gtggggagac ggaacacttt acagatagcg accacgtgga
    37921 agtcttgcat cgtcaccttg gtcgcccttt tatcgacact gtcttggtga atattgaaaa
    37981 agtgcctcag gaatacatga attccaaccg ttttgatgaa tacttggtgc aagtggaaca
    38041 cgattttgta ggtctttgta agcaagtttc gcgcgtgatt tcatctaact tccttcgtct
    38101 ggaaaatggc ggtgccttcc acgatggaga tttgattgtg gacgagttga tgcgcattat
    38161 acaggtgaaa aaatgagttt cacagtagca gtaaaagaag aaatcctggg ccaacaccat
    38221 ctgagctggc atgaattatc tgccattatc aaaatgtctg gtagcatcgg tctctcgact
    38281 tcgggcttga ctttgtcagt tgtgacagaa aatgccaaac tggcccgtca cctctatgag
    38341 tcctttctcc atttctatga aatcaaatct gaaattcgtc accaccaacg gagcaatctt
    38401 cgcaagaatc gcgtctatac cgtttttaca gatgaaaagg tgcaggattt gttaagtgat
    38461 ttgcacttgg cagactcttt ctttggcctg gaaacaggta ttgatgaggc gattttatcg
    38521 gatgaggaag caggtcgtgc ctatctctgt ggcgctttct tggcaaatgg gagcattcgt
    38581 gaccctgagt caggcaagta ccagttggaa atcagttctg tttatctgga ccacgcgcaa
    38641 ggaattgcct cccttctcca acagttttta ctggatgcca aggtgcttga gcgcaagaag
    38701 ggggctgtga cctatctcca gcgagcagaa gacattatgg acttcttgat agtaatcgga
    38761 gccatgcagg cacgtgatga ttttgagcgg gttaagattt tgcgagaaac ccgtaacgac
    38821 ctcaatcggg ctaataatgc cgagacagct aatatcgctc ggacagtttc tgccagcatg
    38881 aagaccatta acaatatcag taaaatcaaa gatatcatgg gcttagaaaa tttgccagta
    38941 gatttgcagg aagtggcgca actgaggatt cagcacccag actactctat ccagcagtta
    39001 gcagatagtc tcagcacccc tctgaccaaa agtggtgtca atcacagact cagaaaaatc
    39061 aataagatag cggatgaatt ataaaaccac gaatcctatg tgactcgtgg ttctttttta
    39121 taaactggta gagtgttttg gttgtacttt ttgttcaggg taaatgtagt tgatagcgtt
    39181 gttgacagca gttggagctt ctccgaggcc tgtcgcaatc agatcaattt ttccgtcata
    39241 gtagcagcag tcaccgatag catagatacc tgcttggctg gattcctgtt tgctgtttac
    39301 gataatcttg tgacggttga ggtcgagccc ccagttttta aggttaccga cagaagattt
    39361 gaaaccatag ttgacaaaga ggtggtctag gtcaatggtt tcagtttcat cagatttgac
    39421 ttttgtgatt tcaagtttat caagtgtttt tccatttcca aggagttggc taggggcgaa
    39481 tggtgtcttg atggttacag atgattcttg caaggcttga acactgtgtt ccaaggcacg
    39541 gaaattatct ctgcggtgaa caagggtagt tggtgcgatt ttttcaaaag ccaaagtcca
    39601 atccacagcc gagtctcccc cgccaagaat cgtcactttc ttaccagcgt attgctgaat
    39661 gttagaaacg tggtagtgga tattttcata gccctcaacc ccttcaagtt ccagcggacg
    39721 tggtttgaag gcaccgccac ccatagcaat gataactgtt ttagtcaggt gacttccttt
    39781 agaagttgtg atggcaaatt cttcttcttg tttgtcaatt tcaagaaccg tttcattgag
    39841 atgaataggg gtatcaaatc catttagctg ttcaatcaag cggttagtca actcttctcc
    39901 agtcaggttt gggaagcctg gtacgtctag gatttccttt tcagggtaga gaatagcagg
    39961 ttgtccacct agctggggaa gagagtcgat gatttgaacc ttggcttggc gtaggtgggc
    40021 ataaaaggct gcaaaaagcc cgacaggacc accacccaca atggtaatat catagagttg
    40081 agacatagtt tctcctttgt tttttctagt cagtttattt tatcatattt ttgttcaatg
    40141 taaaatggac taggataggg aaaaaaatgc cagaaaatgg tataatagga cgaaagtgtt
    40201 ttgataggga ggatatcagg gatgaatttt caacaattat ccaatctgca atattggacc
    40261 agtttgtttg caagtccatg gacgatagct atcaatctga ttgatatttt gattgttgct
    40321 tatattttat accattttac aaaagctatt gcaggaacca agattatgat tttggtacgt
    40381 ggagttttgg tgtttatttt agctcaaatc cttgcaaata tgattggttt gactacgatt
    40441 tcttggttaa tcaatcaaat tattacttat ggggttattg cggcggttgt tatcttctct
    40501 ccagagattc ggactggttt ggaacgtttg ggaagagcga cagatttctt ttccaatgcc
    40561 cctattagtg ctgaggaaca gatgattcgt gcctttgtta agtctgttga atacatgagt
    40621 cctcgtaaaa tcggggcctt ggttgctatt cagcgtgtac gtaccttgca ggagtatatg
    40681 tcgacaggaa ttcccttgga tgctaagatt tctgcagaac ttctcattaa catttttatt
    40741 cccaacactc ccctacatga cggtgcggtg attatcaaag aagaacgtat cgctgtgacg
    40801 tctgcctatc tgcccttgac aaaaaacaca gggatttcca aggaatttgg gaccagacac
    40861 cgggcggcta tcggtttatc agaagtctca gatgccttga cttttgtcgt atcagaggaa
    40921 acgggaggaa tttcgataac ctataatgga aggtttaagc acaacctaac acttgatgaa
    40981 tttgaaacag aattacgtga aatcttactt ccaaaagagg aagtgggtct tagttttaaa
    41041 gaacgattgc taggaggatg gaaacatgaa aaaaaatagt ttatatatca tatcctcact
    41101 cttttttgct tgtgtcttat ttgtctatgc tacggcgacg aattttcaaa acagtaccag
    41161 tgctaggcag gtaaaaacgg aaacctatac taatacagta acaaatgtcc ctattgacat
    41221 acgctataat agtgataagt attttattag cggttttgct tcagaagtat cagtggtctt
    41281 gactggtgca aatcgtctat cgctagctag tgaaatgcaa gaaagtacac gtaaattcaa
    41341 ggttactgct gacctaacag atgccggtgt tggaacgatt gaagttcctt tgagcattga
    41401 agatttaccc aatgggctga ccgctgtggc gactccgcaa aaaattacag tcaagattgg
    41461 taagaaggct cagaaggata aggtaaagat tgtaccagag attgacccta gtcaaattga
    41521 tagtcgggta caaattgaaa atgtcatggt gtcagataaa gaagtgtcta ttacgagtga
    41581 ccaagagaca ttggatagaa ttgataagat tatcgctgtc ttgccaacta gcgaacgtat
    41641 aacaggtaat tacagtggtt cagtaccttt gcaggcaatc gaccgcaatg gtgttgtctt
    41701 accggcagtt atcactccgt ttgatacaat aatgaaggtg actacaaaac cagtagcacc
    41761 aagttcaagc acatcaaatt caagtacaag cagttcatcg gagacatctt cgtcaacgaa
    41821 agcaactagt tcaaaaacga attaaaaata gaaaaggatt ttataacaat gggtaaatat
    41881 tttgggactg atggagtccg tggagaagct aacctagaac taacaccaga attagccttt
    41941 aaactaggac gttttggagg ctatgttctt agtcaacatg aaacggaagc gccgaaagtc
    42001 tttgtaggac gtgacacacg tatttcaggg gaaatgctgg aatcggcctt ggtggcaggt
    42061 ctcctttcag tagggattca cgtatacaaa cttggtgtcc ttgcaacacc agcagtagct
    42121 tacttggttg aaactgaagg agcaagtgcc ggtgtcatga tttctgctag ccacaaccca
    42181 gcccttgata acggaatcaa gttctttggc ggtgatggct tcaaactaga tgatgaaaaa
    42241 gaagcagaaa ttgaagcctt gctagatgct gaggaagaca ctcttcctcg tccaagtgca
    42301 gaaggtttag gaattttggt agattatcca gaaggcttgc gtaagtatga aggatacctt
    42361 gtttcaactg gaactcctct tgatggaatg aaggttgcct tggatacagc taatggagca
    42421 gcttctacca gtgcccgtca aatctttgca gaccttggtg cccaattgac ggttatcggg
    42481 gaaacaccag acggtcttaa catcaacctt aatgttggtt caacacatcc agaagccctt
    42541 caagaagtgg tcaaagaaag tgggtcagct attggtttgg cctttgatgg agacagtgac
    42601 cgcttgattg ctgttgatga gaatggtgac atcgtcgatg gtgacaagat tatgtacatc
    42661 atcggaaaat acctttctga aaaaggacaa ttggctcaaa atacaattgt gacaactgtt
    42721 atgtctaacc ttggtttcca caaggccttg aatcgcgaag gtattaacaa ggcagttact
    42781 gcagttggtg accgctacgt tgttgaagaa atgagaaaat caggttacaa ccttggtggt
    42841 gaacagtctg gtcacgttat cttgatggat tacaatacca caggtgatgg tcaattatca
    42901 gcagttcaat tgactaaaat catgaaggaa actggtaaga gcttatcaga gttggcggca
    42961 gaagtaacga tttatccaca aaaattagtt aatatccgag tggaaaacgt catgaaggaa
    43021 aaggccatgg aagtgccagc tatcaaggcc atcatcgaga agatggaaga agaaatggcg
    43081 gggaacggcc gtatccttgt tcgtccaagt ggaacagaac ccctcttgcg tgttatggca
    43141 gaagcgccta caacagaaga agtagactac tatgttgata ccatcacaga tgtagttcgt
    43201 gctgaaattg ggattgacta aatcctagaa aactagatga aaataaaaaa ttatggtaca
    43261 atagcttata atattgtagc cgatggagaa agacatgaat cttaaacgtg aacaagaatt
    43321 tgttagtcag tatcattttg atgcgcgtaa ttttgaatgg gaaaatgaaa atggagctcc
    43381 tgaaaccaag gtggatgtga atttccaatt gctccaacat gaccaagaaa atcaagtgac
    43441 ttccttgatt gtcatcttga gctttatgat tgtatttgat aagtttgtca ttagtggaac
    43501 aatctcacaa gtaaaccata tcgatggccg tattgtcaat gaaccaagtg aattgaacca
    43561 agaagaggtg gaaaccttgg ctcgtccatg tttgaacatg ctcaatcgtt tgacttacga
    43621 agtgactgaa attgcattag acttaccagg aattaatttg gagttctaat atgaaattag
    43681 ctgttttcac agattcctct gcttatctca gtgcagagac cttgcaaaga gaagacttgt
    43741 ttgtcttgga tattcctgtc aatattgatg gtgaggaata tgtcgaaggc atcaatctgt
    43801 ctgctgagga attttaccaa aaaatggctc aggcttctga attgcctaag accagtcaac
    43861 caagtattgc caagttagat gaaatcttaa cttcgctcaa agaacaaggc tatacacatg
    43921 ccttggggct tttcctatct tctggaattt caggttttta ccaaaatatc cagtatatgg
    43981 tcgatgacta tgagggctta accattgctt tcccagacac tttgattaca agtgctcccc
    44041 taggcatcat ggttgaaagc gtctttaatt ggcgtgatca gggcgatgat tttgccagca
    44101 ttcaggataa gctagccatt caaatcagcc gtacgtcagc ctttatcatg gtagatgact
    44161 tggatcattt ggtaaaaggt ggacgccttt caaatggggc tgccattttg ggcaatttgc
    44221 ttagcattaa gccaatcctt tattttaacg atcaaggtgt gattgaagtt tacgaaaaag
    44281 ttcgtactga aaagaaggcc accaaacgct taattgaaat tatcaaggaa acaacggctt
    44341 caggtcaata ccgggtcatt gtcattcatg ggaatgctcc tgaaaaggct gaagaattgc
    44401 gtcagcattt gcttgatttt ggcttgggtt cggatgtttc acttgctaca tttggtagtg
    44461 tcattggaac gcacctagga gcaggaagta ttgctctggg ttatattcca gtgatttagt
    44521 aggcacttct aggctagtta atcttagcaa ttgaacacgg actacctgcc tcttgaaaaa
    44581 agatgtctgt cttgcctttc gtggaaagtc agtgccattc cctatttttc atgggtggct
    44641 aagatccttt gtatcttgat aattgaacac gcctggaacc ctgtgtgaaa aagatagttc
    44701 ttccaaggag tagacacttc ttgatcagaa ttcctatttt cactttgtgt tcttacaggc
    44761 tttgtatctt agacaggagt agagatgagt attcgagtaa ttattgccgg ttttaaggga
    44821 aagatgggtc aagctgcttg ccagatggtc ttgactgatt cagatttgga cttggtagca
    44881 gttttggatc cttttgagtc tgagtcagaa tggcagggta ttcctgtttt caaggataag
    44941 gctgatttag ctggttttga agcggatgtc tgggtagact ttactactcc agctgttgcc
    45001 tacgaaaata cacgttttgc tcttgaaaat ggctttgctc cagtagttgg aacgactggt
    45061 ttcacgagtg aagaaattgc agagctaaaa gaattttctc gtgcccaaga cttgggtggc
    45121 ctgattgccc ctaactttgc cttgggtgct gtcttactca tgcaatttgc gacgcaggct
    45181 gccaaatatt tcccaaatgt ggagattatt gagctccatc atgacaagaa aaaggatgct
    45241 ccgagtggaa cagccattaa aacagctgag ttgatggcag aggttcgaga gtcaattcag
    45301 caaggtgcag cagatgagga agagctgatt gctggtgctc gtggtgctga ctttgatggt
    45361 atgcgcatcc actcagttcg tttgccaggt ttggtagccc atcaggaagt catctttggc
    45421 aatcaaggag aaggattgac cctccgtcat gactcctatg atcgcatctc cttcatgaca
    45481 ggagtcaatt tgggaattaa agaagttgtc aagcgtcatg agcttgtcta tggattagaa
    45541 cacttattat gagattaacg caaatgcctt ctgaatttca gaaggcttta ccagtattag
    45601 aaaaaattaa agaagcaggc tttgaggctt attttgttgg gggctctgtt cgagatgccc
    45661 ttctccatag ccctatccac gatgtggata ttgcgacgtc ttcttatcca gaagagacca
    45721 agcagatttt tccgcgaaca gccgatatcg gaatcgagca tggaaccgtc ttggtcttag
    45781 atggggatga ggagtatgag gtaacaacct ttcggacaga ggatgtctat gtggactatc
    45841 gcagacccag tgcggtttcc tttgtgcgct cgctagaaga agacctcaaa cgccgtgatt
    45901 tcacagtcaa cgcctttgcc ttggatgaga caggagaaat cgttgacttg ttccatggtt
    45961 tagaagattt ggaaaagcaa gtcttgcgag cagttggagt ggctagtgag cgtttcaacg
    46021 aagatgcttt acggattatg cgtggtttcc gttttcaggc tagtcttggt tttgcacttg
    46081 agccagaaac atttaaagct atgaagacct tgacgccgct tttggagaaa atttctgtag
    46141 agcgtacctt cgtcgagttt gataaactct tgctggctcc attttggaga aggggcttgg
    46201 cttccatgat tgagagtcaa gcttatgact atatccctga tatggcatct agccagaaca
    46261 agctcaacag actgtttgat ttggagactg attttacttt tgaatcctct gaacaagcct
    46321 gggcggcttt actatgggct ttggagattg aaaatgcgca gtcatttttg aaatcttgga
    46381 agacctcacg ccagtttgcc aagcaagttc aggatttgct gattattttg gctctgcgtg
    46441 aaaatggaga attgagcaag cgagattgtt atcgctttga catagatttg cttttacagg
    46501 ctgaaaatct tcgtcaagct cgaggaaaag aagtcaaccc acaagccatc acagaaaaat
    46561 accaaagctt gactatccat gacaagaaag atattcagat taatggcgat attttgatca
    46621 aggaatatgg ctatcagcca ggcccagact tgggagagat tttaacagag attgagtttg
    46681 ccattgtcga tggagaattg gagaataatc gtgaagccat ccatgcttac ctgagggaga
    46741 aaaaatgagt gattttatcg ttgaaaaact aagtaaatcc gttggtgaca agaccgtttt
    46801 tagggatatt tcctttatta tccatgactt agacagaatt ggtttaatcg gtgtcaatgg
    46861 gactggcaag accacccttt tggacgtcct ttctggtgtt tctggctttg atggggatgt
    46921 cagtcctttt tcagctaaaa atgattacca gattggttac ttgactcagg atcctgattt
    46981 tgatgataga aagacagttt tggatacggt tctatctagt gaactcaagg aaatccagct
    47041 cattcgtgag tatgaattga ttatgctcga ctatagtgag gacaagcagg cgcgtttgga
    47101 acgtgtcatg gcagagatgg actctctcca agcttgggaa atcgagagtc aggtcaagac
    47161 cgttcttagc aaattgggca ttcaagactt atctactcct gttggggaat tgtcaggtgg
    47221 tctgagaaga cgggtacagt tggcacaagt cttacttggt aaccacgacc tcttgctttt
    47281 ggatgagccg accaaccatc tggatattgc gattattgag tggctgaccc tctttttgaa
    47341 aaattctaag aagaccgtcc tttttatcac tcacgatcgt tatttcttag acgctttgtc
    47401 aacacggatt ttcgagttgg atcgtgcagg cttgaccgag taccggggaa attaccagga
    47461 ctatgttcgc ctaaaggcgg aacaggatga gcgcgacgcg gctcttcttc acaaaaaaga
    47521 acaactctac aaacaagaat tggcctggat gcgcagacaa ccgcaggcgc gtgcgaccaa
    47581 gcagcaggcc cgtatcaatc gattccatga tctgaaaaag gaagtttcag gcagtagtgc
    47641 tgagacagac ttgaccatga actttgaaac cagtcggatt gggaagaaag tcatcgagtt
    47701 tcaggctgtt tcctttgcct atgaaaacaa gcctattttg caagatttta atctcttggt
    47761 gcaggccaaa gaccgtatcg gaatcgttgg ggacaatggt gttggaaaat caaccctgct
    47821 caacttgatt gcaggaagtc ttgagccaac taaaggacaa gttgtgattg gggaaactgt
    47881 tcgcatcgcc tatttctctc agcaaattga gggtttggat gaaagcaaga gagttatcaa
    47941 ttacctgcag gaagtggcag aagaggtcaa gaccagtggc ggttctacga cttcgattgc
    48001 agagttgctg gagcagttcc ttttcccacg ttcgacgcat ggaaccttga ttgagaaatt
    48061 gtcagggggt gagaaaaaac gtctttatct cctcaaactg cttttggaaa aaccaaatgt
    48121 tcttctttta gacgagccaa ccaatgacct agatattgca actttgacag tcttagagaa
    48181 tttcttgcaa ggttttgcag gtcccgtttt aacagtcagt cacgaccgct atttcttgga
    48241 taaggtagcg accaagattc tcgcttttga ggatggcaag attcgtcctt tctttggtca
    48301 ttacaccgac tatcttgatg aaaaagcttt tgaaacagat atggccaatc aagtgcaaaa
    48361 ggccgaaaag gaaaaagtgg tcaaggttcg agaagacaag aaacgcatga cctaccaaga
    48421 aaagcaggag tgggcaagta ttgaaggtga tattgaagcc ttggaaaaac gtatcgctgc
    48481 tattgaagag gaaatgcagg ctaacggctc tgactttggt aagctggcta ctctccaaaa
    48541 agaattggat gagaaaaatg aagcactcct tgaaaaatac gaacgctatg agtatctcag
    48601 tgaatttgat agttgatact attcgaaaat caaagtgcaa actatgtcgg cttcacattg
    48661 ccgactcaag tacagcctgt gaccggcttc ctaatttgca ctttgatttt tattgagtat
    48721 aaacgaatag aaaaatcccg tctcatgaaa ggatttctct attctttttt ggtttcttga
    48781 tgaaagatat tttgccaggt ttcgtggcga cgccagatac tggtatggat gataccatct
    48841 aactcatagc agataagttt ggttttttga ctgatggaag taatttgaat gtccttgata
    48901 gcagaattta actctttttt ggtcttataa gcctctttat ccatctgctc tccatcttgg
    48961 cgaatataga caaaatcatc agtcaagagt tcttctagtt ggtttccttg gtcgatcaat
    49021 tgttcacgca taagcaattt tttatagaca ttgtctaaaa tttcatcctc agggataggt
    49081 gctggttcga tatggacatc ggtatcaaag acgccaaaac gattctccag catagactcg
    49141 acctgatccg cgatttcatg gctttcaaaa acagacaagt caggattcat ctctagtgta
    49201 atatccaggt agatgttgct accgtaggtg cgacctcttt gcgatttgac cttgctgatt
    49261 ttgggaattt ccatgatagc cttttggtag tcctcgagca ggcggtcgtc aaagccatct
    49321 gaaagactaa aggaagactc gatgaagata tcataggcag tcttcaagat aaagaaagtg
    49381 atgatgatag caaccagttt atccacaatc ggataattga aactactagc taggatggca
    49441 atggcggttc caagtgaggt aacagcgtca gaaagattgt ccttagcagc tgccttcagc
    49501 gccttggagt tggatttctt actgaggcga gtattgtaga gatagaccac aaacataatc
    49561 gctgcagaaa tgattcctag agttgcacca agaggatcaa tgaccgtttc ttcccgactg
    49621 agaatctttt gaatggtatc tcttagaaca tcgaaaccga catagaacat gatgatagaa
    49681 gtgatcaagc ttgccaaatc ttcaatcttc caatgaccaa aacggtggtc acggtctgca
    49741 ggctggcgcg ccatccgaat cccgattaag agggccacat ttccaatgat gtccgatacg
    49801 ttattaaaac catcggccac caaactggat gaatgaagga gatgaccagc tgctaatttg
    49861 gctgcagaca agatcaaata ggtcgaaata ctgataatgg ctccacgctc agctaacttg
    49921 agatttgaga tagattgctt cattcaactt cctttctagt cacatagcat tctaccatta
    49981 tactggtttt caaggaaaaa ttcaaataag gcagttttta ctctttgaaa actccccaaa
    50041 atttggtata atggtactac tcaagggagt agctggcaga aacctgtgat agtgtcgtca
    50101 ttccgaattt tatgctgaaa agtatgcttt ccggccctat cttaaacagc gagacttgtt
    50161 atgattaaca ggtctctttt tgtttgtcgt gaaaagatat gacagaaaga gagtctgttt
    50221 tgcacacaat gtcaaggagg agacacatgt caaaagaaca aaaacgccaa gcgttttata
    50281 ctcagagccc tgaagaggtc ttgcaggctg tggatgcgac cgagcaaggt ttgtcatcaa
    50341 gtgaggcgga aaagcgcctt gccgaatttg ggcacaatga actcgaagaa ggcgagaaac
    50401 gatcaatctt ggtcaaattc atcgagcaat ttaaggattt gatgattatc atcctagttg
    50461 cggcagcaat cttgtcagtc gtgacttctg gtggggaaga tatcgcagat gccattatca
    50521 tcctagctgt ggttatcatc aacgctgcct ttggtgttta ccaagaagga aaagctgaag
    50581 aagctattga agccctcaaa tccatgtcta gtccagttgc ccgcgttctt cgtgatggac
    50641 acatggcaga gattgactct aaagaattgg taccaggcga tatcgttgcc cttgaagcag
    50701 gtgacgtggt accagcggac ctacgtttga tagaagccaa ctctcttaaa attgaagaag
    50761 cagccttgac aggtgaatct gtaccagtcg aaaaagactt gtcagtcgag cttgcgacag
    50821 atgctggtat tggtgaccgt gtcaacatgg ccttccaaaa ctcaaacgta acctatggtc
    50881 gtgggatggg tgttgttgtc aatacaggta tgtacactga agttggtcat attgctggta
    50941 tgcttcaaga tgcggatgag actgatacac cactcaaaca aaatttgaac aacctttcta
    51001 aggtcttgac ctatgctatc ttggtcattg cccttgttac ttttgtagtg ggtgtcttca
    51061 ttcaagggaa aaatccactt ggtgagttgt tgacttctgt tgcccttgcc gttgcagcca
    51121 ttccagaagg acttcctgct atcgttacca tcgttctttc tcttggtact caagttttgg
    51181 ccaaacgaca ttccatcgtt cgtaagttgc cagcagttga aacacttggt tcaactgaaa
    51241 tcatcgcttc tgataagact ggtacgctga ctatgaacaa gatgacagtc gaaaaagtct
    51301 tctacgatgc ggttctacat gactcagctg atgatattga actaggtctt gaaatgccac
    51361 tacttcgttc agttgtcttg gccaatgata cgaaaatcga tgtggaaggt aacttgattg
    51421 gtgacccaac cgaaacagcc tttatccaat atgccttgga caagggctat gatgtcaaag
    51481 gtttcttaga gaaatatcct cgtgtagctg aattgccatt tgactctgac cgtaagctca
    51541 tgtcaacagt tcacccattg ccagatggtc gtttccttgt agcagtcaag ggtgcgccag
    51601 accaactctt aaaacgttgt cttcttcgtg ataaggctgg ggatattact ccgattgatg
    51661 agaaggttac aaatctcatt catacaaaca attctgaaat ggctcatcaa gccttgcgtg
    51721 tccttgcagg tgcttataag attatcgata gtattccaga aaatctcact tctgaagagc
    51781 ttgaaaatga tttaattttt actggtttga ttgggatgat tgaccctgaa cgtcctgaag
    51841 ccgctgaggc tgttcgtgtg gctaaggaag cgggaatccg tccaattatg attacaggtg
    51901 accaccaaga cacagcggaa gccattgcca aacgtttggg aatcattgac gcaaacgata
    51961 cagaaggtca cgttttaact ggtgctgaac tcaatgaact gtcagatgaa gaatttgaaa
    52021 aagtcgttgg tcaatactct gtttatgccc gtgtgtctcc agaacacaag gttcgtatcg
    52081 tcaaggcttg gcaaaaacaa ggtaaagtcg ttgccatgac aggtgacggt gtcaatgacg
    52141 cgccagctct gaaaacagcc gatatcggta tcggtatggg aatcactggt acagaggttt
    52201 ctaagggggc ttctgatatg attcttgcag atgataactt tgcgactatt atcgtcgcag
    52261 tggaagaagg acgtaaggtc ttctcaaaca ttcaaaagac tattcagtac ctactttctg
    52321 ctaatactgc tgaagtatta accatcttcc tatcaacctt gtttggttgg gatgtcttac
    52381 agccggttca tcttttgtgg atcaacttgg taacggatac cttcccagct atcgctcttg
    52441 gtgttgaacc tgcggaacct ggtgtcatga atcataaacc acgtggacgc aaggcaagct
    52501 tcttctcagg tggtgttttg agttctatca tttatcaagg tgtactccaa gcagctcttg
    52561 ttatgagtgt ttatggcctt gcgattgctt acccagttca tgtgggtgac aatcatgcta
    52621 ttcatgcaga tgccctaacg atggcctttg caacccttgg tttgattcag ctcttccatg
    52681 cctacaatgt caagtctgtc taccaatcca tcttgacagt tggcccattc aagtctaaga
    52741 cctttaactg gtccatcttg gtatccttta tccttctcat ggcaacaatc gttgtagaac
    52801 cgcttgaagg aatcttccac gtaaccaaac tagacttgtc acaatgggga attgttatgg
    52861 ctggaagctt ctcaatgatt atcatcgtcg aaatcgttaa gtttattcaa cgcaaactcg
    52921 gttttgacaa gaatgcgatt taagggaggt ttcggatgtc agcatatcaa ttaccgaccg
    52981 tatggcagga tgaagctagt aatcaaggag cttttacggg gctaaacaga ccaacagcag
    53041 gtgcccgttt cgaacaaaac ttgccaaaag gagaacaagc ttttcagctt tattcactgg
    53101 gaacaccaaa tggtgtgaag gttactatct tattggaaga attactagaa gctggtttta
    53161 aggaagcggc ttacgacttg tataagattg ctatcatgga tggggatcaa ttcggctcag
    53221 actttgtgaa gctcaatcca aattccaaga ttccagcctt attggaccag tcaggtactg
    53281 aaaacgtaag agtctttgag tctgctcata ttcttcttta ccttgctgag aaatttggag
    53341 cctttttacc aagtaatcct gtggaaaagg tagaagtttt gaattggcta ttctggcaag
    53401 caggtgcagc accttttcta ggtgggggat ttggacattt cttcaattat gctcctgaaa
    53461 aattggaata tcctattaac cgttttacga tggaagtgaa acgccagttg aatttattgg
    53521 ataaggaatt ggctcagaaa ccttatattg caggcaatga ctatacgatt gcagatattg
    53581 ctatctggtc ttggtatgga cagttagttc aaggaaatct ttaccaaggt tctgcaaaat
    53641 tcttggatgc ctcaagttat caaaatctag taaaatgggc agaaaaaatt gccaatcgtc
    53701 cagctgttaa gcgtggcttg gaagtaactt atacagaaat taaatagcaa aaaagtcatc
    53761 ttcggatggc tttttgctac agttgaggga aagggtttgc agttatcggc ttttgtgcta
    53821 taattgctat aatatagtaa aaagcaagag gagtgtgagg aagtggaaaa gaaaattgtg
    53881 aaggatatct tatttttatc tcaagtgtct cagccggcaa gtcaggagga cctttatctt
    53941 gccagagatt tgcaggatac actcttagca aatcgtgata cctgtgttgg tctagctgcc
    54001 aatatgattg gggtgcagaa gcgcgtgatt atctttaatc ttggcttggt tcccgtggtc
    54061 atgtttaacc cagtgcttct gtcctttgaa ggatcctatg aggcagaaga aggctgtttg
    54121 tccttggtag gtgtgagatc aactaagcgt tatgaaacca taagggttgc ctatcgtgac
    54181 agcaagtggc aggaacagac cattaccttg acaggcttcc cagctcagat ttgccagcat
    54241 gagctggatc acttggaagg acgaatcatt taggaggaaa gcaaatgaaa cgaatagtct
    54301 ttgaacttat ttttatcgca acgacctggt atatcttttt accgcccctt aacctgacca
    54361 gctgggaatt tctcttcttc ctctgtgggc atttgttagt tgtggcaatc ttatttggct
    54421 ttggcaaggg gataaacctt gtcaaaacgg ttcatgtgcg ccacggtaag gcggaagctg
    54481 ccttaaatct tgagggtttc aaaatcaatc ggttagggaa aattctgtta gcttcgattg
    54541 gaggaattct tctcttggca gctttggttt ccttggtaac ttccagcatg tttcaggcta
    54601 aaaattatgc caatgtagtc acggttacgg aaaaagactt tactgaattt cctaagagtg
    54661 acaccagtaa ggttcctatc ctagatagaa gtactgctga aaaaattgga gaccgctact
    54721 tgggttccct aaccgataag gtgtcgcaat acgtagcggc agatacctat acccaattga
    54781 caattgatgg gaaaccttat cgggtcacac cactagaata tgcagaccct atcaaatggt
    54841 ttaacaatca agccaaggga atcggtgagt atatcaaggt agacatggta actggaaatg
    54901 cggatttggt ggacttgaag acaccaatca agtattcaga ctcggagtat tttaaccgtg
    54961 atgtcaaacg tcacctgcgc ttgaagtacc cgaccaaaat ctttaaaact ccatcttttg
    55021 aggtgaacga tgagggcaat cctttctatg tagcaacggt ttaccaaaag caatttggac
    55081 ttgcggttcc tcgtcctgct tcagtcatta tcttggatgc tacaaatgga gaaaccaagg
    55141 aatacagctt atcagatgtt ccagaatggg tggacaggat ctatccagca gaggaaacca
    55201 ttgagcaaat caactacaac ggcaagtaca aggacggttt cttgaatgcc atgatttcca
    55261 agaaaaacgt gacccagact accaatggct ataattactt gtctatcggt aatgacatct
    55321 atctctacac aggtgtgacg tcggctaatg cggatgagag taatcttggt ttcatccttg
    55381 aaaatatgcg aacaggagaa attactaagt atagcttggc ttctgcgaca gaagaatcag
    55441 cccgtgaatc agcagaaggt gctgttcagg agaaatccta caaagcaacc ttcccaatcc
    55501 tcatcaacct caatgacaag cctctctaca tcatgggctt aaaggacaat gctggcttgg
    55561 tcaaagagta cgccctggta gacgcagtcg agtaccaaaa tgttatcgtt gctactacag
    55621 tggaagagat gctcagcaag tatgccaata aaaacgacct tgaaattgac aatgcaacga
    55681 cagaaagcat caagggagta gtagcagacc tcaaatcagc tgttatcaag ggagacactg
    55741 tctacttctt taaagttgat ggcaagatat acaaggtaaa ggcttcagta tccgatgacc
    55801 ttccttacct tgaaaatggt aaaaccttcg aaggtcaagt aggaaaagac aattatctca
    55861 agacctttaa agtacaataa tactcttcga aaatctctag ctctatctgc aacctcaaaa
    55921 cagtgttttg agcagcctgc ggctagtttc ctagtttgct ctttgatttt cattgagtat
    55981 aaaaaaggtt tatttaataa atatatgtta taataaggta aattaagcct aatggaggac
    56041 aaagaaatgg gtttttattt gatggttgct tcaatgctac tcggactgct cgctttgaag
    56101 ataggttttt ctcagttcaa ggagaaaaaa gataaattct tatctatctt aacaagttta
    56161 gctggcatag cccttgttct cgcggctgtc tggttaggat ggcccaaata aatagaaaaa
    56221 cctctgtcaa accgaggttt ttcaaatgaa tttcttggtt gtggctaaaa aatatgctac
    56281 actatcaata tgaaaatttt aatcccaaca gcaaaagaaa tgaacacaga cttgccaagt
    56341 atcgaggcaa ttcctttaaa accagaaagt cagaccgtgc ttgatgcctt ggctctctat
    56401 tctgccagtc aattggagag tttctacaag gtatcagctg agaaagcggc ggaagaattt
    56461 caaaatatcc aagctttgaa aaggcaaact gctcaacact atccagcctt gaaacttttt
    56521 gatgggctta tgtaccgcaa cattaagaga gataagctga ccgaggcgga acaagattat
    56581 cttgaaaatc atgtcttcat tacctcggct ttgtacggtg ttgttccagc cttgtcaccc
    56641 atggctcctc accgtttgga ttttttgatg aaattaaaag tcgctggtaa gactttgaag
    56701 agccattgga aggcagtcta tgatgaagct ctgaagaagg aagaagtgat tttctctctc
    56761 ttgtcatcag agtttgagac tgtattttct aaggaaatca gagcaaagat ggtgaccttc
    56821 aaattcatgg aggatagagg cggtcagctg aagattcact caaccatctc caagaaagcg
    56881 cgcggggcct ttctaacagc tttaatagaa aatcaagtac aaactgtggg ggaagcacgt
    56941 cgcttgaact ttgctggatt tgtttaccga gaagatttgt cacaaccaca gggattggtt
    57001 tttgtaaagg aagtatagaa atcagataag agaaaagtca gcacgattat gctgactttt
    57061 ttgataactt ataaagtatt ggacaaatga atctcatttg ataaggtgtt aagatgatta
    57121 accaaattca tgattgacag atgatttcgg caaataaact tctttgtaat ccctggaata
    57181 tagaaattag acacgatgat atcgtaatct gtctgattta aaatctcagg acttgttttt
    57241 aattcatccc aaatatcgaa ggtaaagcgg ttattacagt aataggaaag catatcaaca
    57301 aaggtaaggg atatagcatg gtcaaagtta ctgataatca aaaccttgat aggtggtcga
    57361 ttttctaaca actgagtaga gaggttttca gcatgggtta agatggtgta catcaagtgt
    57421 tccaactgtt caggatggtc atgctgtcca attgcctgac ggtattgagc caattcttga
    57481 cgcgcacttc ccataaagtc tgggaagtaa acttcaaatt ttttaatcgt caatgctttt
    57541 tgttcaaata agattggggt agaaaagatt tcctgtcttt caaagaaagc agtgttgtgt
    57601 aaatgccaaa tcagctcatc gtggttagta aaagtaatct tatattgttt tgataggcct
    57661 tctaatattt gactaagtaa ttggtaagag tatcttgctt gactgttatc ttctaaagag
    57721 ttgaagaatt cttgaggatc taggaaaata tcattctgaa gaaaagaaat aaaaatttga
    57781 gcgattgtat ctggagtcat ttcaagccca aaatgtttag agaaataagc aagtgtctcc
    57841 tggaaattcg gaatgctctt taaaagagga taatattctt tgtaaaagtg gttggggagg
    57901 tcaatgaagt gaccattttt aaccctgtgg atactgatag ctatcatcag tttgtacatt
    57961 ctataaattg aaaatcgcat tggataattt gtgattttat agaagaagtc agcaaactct
    58021 gttaaatctt cctcaggaag gtcaggaaaa ggccaatcta ggaaataata gcgctcagaa
    58081 aagtattggg caaaaaagta acgaatatcg atctcatttc cacgtatctc ggatggagta
    58141 aaagataatt caatttgaaa ctgagatgaa agaacctttg taatatttct gcccagtcga
    58201 tagaggttgg cactactaaa gtaaagattt tctattgttc gataaatagg tagcccctca
    58261 ttgaagaaca tatactctag taattgaaaa gattgagaat tagcaagaaa gtattgataa
    58321 atatcttcta cactagtgtt aacttctaaa tctatcatga ttccgttaac agaagattgg
    58381 atgttaatag atggaaaggc aatacgtaat tcatttaaat cactttttaa gattcgttct
    58441 gtacatccta atttttcggc tagttcatgt aatttcatcc aattacgatt ctgaattagg
    58501 gtttccatta agcgtaattg tctttgaact tttgtggata aaaggtttct catacgggta
    58561 tttcctccat aattcattat atcttttttt gtctaagtag tataaaaaaa ttagagcaag
    58621 aaaattgtcg ttagtaatcg ga
//
LOCUS       DAVKGC010000002        55478 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKGC010000002 DAVKGC010000000
VERSION     DAVKGC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 55478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.002"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(202..492)
                     /locus_tag="VW049_000060"
     CDS             complement(202..492)
                     /locus_tag="VW049_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431265.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEYKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(480..731)
                     /locus_tag="VW049_000061"
     CDS             complement(480..731)
                     /locus_tag="VW049_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9431266.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            935..1012
                     /locus_tag="VW049_000062"
                     /pseudo
     CDS             935..1012
                     /locus_tag="VW049_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(1130..2014)
                     /locus_tag="VW049_000063"
     CDS             complement(1130..2014)
                     /locus_tag="VW049_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9431267.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(2159..3310)
                     /locus_tag="VW049_000064"
     CDS             complement(2159..3310)
                     /locus_tag="VW049_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9431268.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(3456..5222)
                     /locus_tag="VW049_000065"
     CDS             complement(3456..5222)
                     /locus_tag="VW049_000065"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9431269.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYCACQLLGPLHK"
     gene            complement(5305..8421)
                     /locus_tag="VW049_000066"
     CDS             complement(5305..8421)
                     /locus_tag="VW049_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9431270.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNDYEVFTQHIDSLESNNLSIDLKGLKGKKVRISLRSAGIPLSLA
                     EVEVYTYK"
     gene            complement(8431..10725)
                     /locus_tag="VW049_000067"
     CDS             complement(8431..10725)
                     /locus_tag="VW049_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9431271.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(10784..11584)
                     /locus_tag="VW049_000068"
     CDS             complement(10784..11584)
                     /locus_tag="VW049_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9431272.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(11581..12354)
                     /locus_tag="VW049_000069"
     CDS             complement(11581..12354)
                     /locus_tag="VW049_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9431273.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(12379..12849)
                     /locus_tag="VW049_000070"
     CDS             complement(12379..12849)
                     /locus_tag="VW049_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9431274.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(12840..13271)
                     /locus_tag="VW049_000071"
     CDS             complement(12840..13271)
                     /locus_tag="VW049_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9431275.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            complement(13264..13704)
                     /locus_tag="VW049_000072"
     CDS             complement(13264..13704)
                     /locus_tag="VW049_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU9431276.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            complement(13716..14354)
                     /locus_tag="VW049_000073"
     CDS             complement(13716..14354)
                     /locus_tag="VW049_000073"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU9431277.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            complement(14469..15872)
                     /locus_tag="VW049_000074"
     CDS             complement(14469..15872)
                     /locus_tag="VW049_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9431278.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            16048..16821
                     /locus_tag="VW049_000075"
     CDS             16048..16821
                     /locus_tag="VW049_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9431279.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(17949..19454)
                     /gene="adcA"
                     /locus_tag="VW049_000076"
     CDS             complement(17949..19454)
                     /gene="adcA"
                     /locus_tag="VW049_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9431280.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVTKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYVEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(19464..20270)
                     /locus_tag="VW049_000077"
     CDS             complement(19464..20270)
                     /locus_tag="VW049_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9431281.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(20263..20967)
                     /locus_tag="VW049_000078"
     CDS             complement(20263..20967)
                     /locus_tag="VW049_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9431282.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVSHA"
     gene            complement(20967..21407)
                     /gene="adcR"
                     /locus_tag="VW049_000079"
     CDS             complement(20967..21407)
                     /gene="adcR"
                     /locus_tag="VW049_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9431283.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(21562..22830)
                     /gene="dltD"
                     /locus_tag="VW049_000080"
     CDS             complement(21562..22830)
                     /gene="dltD"
                     /locus_tag="VW049_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9431284.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFIPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(22823..23062)
                     /gene="dltC"
                     /locus_tag="VW049_000081"
     CDS             complement(22823..23062)
                     /gene="dltC"
                     /locus_tag="VW049_000081"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9431285.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(23076..24320)
                     /gene="dltB"
                     /locus_tag="VW049_000082"
     CDS             complement(23076..24320)
                     /gene="dltB"
                     /locus_tag="VW049_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9431286.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQFLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(24317..25867)
                     /gene="dltA"
                     /locus_tag="VW049_000083"
     CDS             complement(24317..25867)
                     /gene="dltA"
                     /locus_tag="VW049_000083"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9431287.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEDLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(25888..26019)
                     /locus_tag="VW049_000084"
     CDS             complement(25888..26019)
                     /locus_tag="VW049_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9431288.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(26250..26954)
                     /locus_tag="VW049_000085"
     CDS             complement(26250..26954)
                     /locus_tag="VW049_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9431289.1"
                     /translation="MMSELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEVEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(27022..28848)
                     /gene="glpO"
                     /locus_tag="VW049_000086"
     CDS             complement(27022..28848)
                     /gene="glpO"
                     /locus_tag="VW049_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9431290.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPAANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLLDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVKAALAQNDLAELKN"
     gene            complement(28890..30398)
                     /gene="glpK"
                     /locus_tag="VW049_000087"
     CDS             complement(28890..30398)
                     /gene="glpK"
                     /locus_tag="VW049_000087"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9431291.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(30557..31984)
                     /locus_tag="VW049_000088"
     CDS             complement(30557..31984)
                     /locus_tag="VW049_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9431292.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLDSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            32149..33021
                     /gene="hslO"
                     /locus_tag="VW049_000089"
     CDS             32149..33021
                     /gene="hslO"
                     /locus_tag="VW049_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9431293.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            33008..33988
                     /gene="dusB"
                     /locus_tag="VW049_000090"
     CDS             33008..33988
                     /gene="dusB"
                     /locus_tag="VW049_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9431294.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(34218..35963)
                     /gene="pspC"
                     /locus_tag="VW049_000091"
     CDS             complement(34218..35963)
                     /gene="pspC"
                     /locus_tag="VW049_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEU9431295.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEEVGGRN
                     TPTVTSSGQDISKKYADEVKSHLEEMLSGIKLDRRKHTQNFNFNKKLSIIQTKYFYEL
                     NVLKEKSKKEELTSKTKEELTAAFEKFKKDTLKLGEKVAEAEKKVAEAEKKAKAQKEE
                     DRRNYPTNTYKTLELEIAESDVEVKKAELELLKEEAKTRNEDTINQAKAKVESKQAEA
                     TRLEKIKTERKEAEEEAKRKADAKLQEANVATSEQGEPKRRVKRGALGEQATPDKKEN
                     DAKSSDSSVGEETLPSPSLKPEKKVAEAEKKVAEAEKKAKDQKEEDRRNYPTNTYKTL
                     ELEIAESDVEVKKAELELVKEEAKEPRNEEKVKQAKAEVESKQAEATRLEKIKTDRKK
                     AEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPAPKPENPAEQPKAEKPADQQAE
                     EDYARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQN
                     NGSWYYLNANGSMATDWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNT
                     TVDSYRVNANGEWVN"
     gene            complement(36042..36569)
                     /locus_tag="VW049_000092"
     CDS             complement(36042..36569)
                     /locus_tag="VW049_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9431296.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            complement(36652..37983)
                     /gene="hk06"
                     /locus_tag="VW049_000093"
     CDS             complement(36652..37983)
                     /gene="hk06"
                     /locus_tag="VW049_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9431297.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL"
     gene            complement(37980..38633)
                     /gene="rr06"
                     /locus_tag="VW049_000094"
     CDS             complement(37980..38633)
                     /gene="rr06"
                     /locus_tag="VW049_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9431298.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            complement(39275..41707)
                     /locus_tag="VW049_000095"
     CDS             complement(39275..41707)
                     /locus_tag="VW049_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9431299.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKVTYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            complement(41709..42167)
                     /locus_tag="VW049_000096"
     CDS             complement(41709..42167)
                     /locus_tag="VW049_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9431300.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            complement(42286..43014)
                     /locus_tag="VW049_000097"
     CDS             complement(42286..43014)
                     /locus_tag="VW049_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431301.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPQGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(43014..44021)
                     /locus_tag="VW049_000098"
     CDS             complement(43014..44021)
                     /locus_tag="VW049_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9431302.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(44060..44818)
                     /locus_tag="VW049_000099"
     CDS             complement(44060..44818)
                     /locus_tag="VW049_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9431303.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            complement(44781..45071)
                     /locus_tag="VW049_000100"
     CDS             complement(44781..45071)
                     /locus_tag="VW049_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9431304.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(45142..45244)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(45327..46445)
                     /gene="cbpD"
                     /locus_tag="VW049_000101"
     CDS             complement(45327..46445)
                     /gene="cbpD"
                     /locus_tag="VW049_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9431305.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNYQNQSGNISSYGSNNSSTV
                     GWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQTGWLKENGSWYYLGSSGAM
                     KTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(46534..46800)
                     /locus_tag="VW049_000102"
     CDS             complement(46534..46800)
                     /locus_tag="VW049_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431306.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(46802..48154)
                     /gene="dnaB"
                     /locus_tag="VW049_000103"
     CDS             complement(46802..48154)
                     /gene="dnaB"
                     /locus_tag="VW049_000103"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9431307.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(48198..48650)
                     /gene="rplI"
                     /locus_tag="VW049_000104"
     CDS             complement(48198..48650)
                     /gene="rplI"
                     /locus_tag="VW049_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9431308.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(48647..50620)
                     /locus_tag="VW049_000105"
     CDS             complement(48647..50620)
                     /locus_tag="VW049_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9431309.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDETSESDISQINSFVANFISEFSEKHMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(50756..51304)
                     /gene="raiA"
                     /locus_tag="VW049_000106"
     CDS             complement(50756..51304)
                     /gene="raiA"
                     /locus_tag="VW049_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9431310.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(51384..52046)
                     /locus_tag="VW049_000107"
     CDS             complement(51384..52046)
                     /locus_tag="VW049_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9431311.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(52043..53341)
                     /locus_tag="VW049_000108"
     CDS             complement(52043..53341)
                     /locus_tag="VW049_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9431312.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            53397..54032
                     /locus_tag="VW049_000109"
     CDS             53397..54032
                     /locus_tag="VW049_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9431313.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHGLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            54281..55201
                     /gene="cysK"
                     /locus_tag="VW049_000110"
     CDS             54281..55201
                     /gene="cysK"
                     /locus_tag="VW049_000110"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9431314.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIELSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
BASE COUNT        16446 a        12353 c         9415 g        17264 t
ORIGIN      
        1 atctgaatct ttgagatgag ctttataaat cgcttttttc agtttttgca ctggtgtttc
       61 gataaactca aactttttag ccgtggtatt gcctgatttt atagtatatt gaaactagaa
      121 tagtacacct ctacttctaa aacattttta gaaatcgatt tgactgtcct gatcgatttg
      181 tcctgttctt atttcatttt actatatttg agccacttcg tctttaacgg ctttattcat
      241 aagctcttgt aatttttctt tactatcaat tacttctgat tttccgttgt aatttattgt
      301 aataggtttt aacttaccta atttctcgac acgctcatta atttgatctt ttttgaaggc
      361 tgcatatgtt tttcctaaga ttttttcgaa aatatattta tcagatagcg gtttgtattc
      421 ttcttcagct tggtttttgt attgatttga aacataagga acaaatcctt catagtaacc
      481 taatgctccc ataagttcaa aagcttgttt tctaattcaa accattgcaa ctcagatttc
      541 agcttttcag ataaatcctg ctcatccaaa taatgacttg aaattagtgc tgaactcgtt
      601 tctgtatcct gtacaggctg agcacccata ccagcaaaaa ataaactcgt tcctagcaag
      661 accgaacaag ctcctattgc atatttcctc aaagaaaaac gctgctttct ctcaaattga
      721 aattctttca tcccatctcc catcattcat tattactgta tattttgtat atcagaaata
      781 gtttgtattc acaaatcttt ctagttattc ccttatcatt cctaattaag ggagatgaca
      841 tacaataatt tttagttaaa tgtatatcga tgttttttgt ttttcttaat aaacgcaata
      901 caaaaagagc ctgttgccaa gctctttata ctcaatgaaa atcaaagagc aaactaggaa
      961 actagccaca ggttgctcaa aacaccgttt tgaggtagta gataagactg acgaagtcag
     1021 ctcaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagtaaca tctatacggc
     1081 aaggcgacgc tgacgtggtt tgaagagatt ttcgaagagt attagtctat tatttcttct
     1141 cagcgcgaag ggctgacaag atttgtgttc ggatatcatc cacaccattt ggagtatttg
     1201 gtaaaaagat ggtttgattt cctttagagg caaaggtatt caaggtatcc aaatactggt
     1261 tggtcaagag gatagacatg atttgttctt ctgtcatgcc aacattggct tccttgagtt
     1321 cggtgataga ctctgccaat ccatccacaa tcgccttacg ttgttgggca atccccacac
     1381 catgaaggcg gtctttttct gcttctgctt cagctgcagt gacaatttta atcttgtcag
     1441 cttccgccaa ttcttgtgct gcgacccgct tacgttgcgc cgcattgatt tcattcatgg
     1501 attgcttaac ttctgcatct ggttcgacct tggtaatcaa ggttttcacg ataatgtagc
     1561 cgtaagtggt catttcttct gctacttggt gttgaacctc aagggcaatc tcatcttttt
     1621 tctcaaacaa ttcatccaag gttaattttg gaacagaaga gcgaagagca tcttcgatat
     1681 aagatttaat ctgagattct ggacgtatga gtttatagta agcatctgtc acgctctgct
     1741 cgttgacacg gtactgagtc gctacattca tcataacgaa cacattgtcc ttggtcttag
     1801 tctcaaccac aatatcactt tgcaacaagc gcaactgaat ccgtgctgca atcgagtcaa
     1861 tcccaaaagg caagcgaata tgaataccgc tattagcaac cttttggtat ttcccaaagc
     1921 gttcaataat cgccaccgac tgctgacgaa ccacataaac tgtactcagt gtgactatca
     1981 ccaataggag cacacaaaca atcagaaaaa tcatgaaaaa tattgccata atggaacctc
     2041 cacaagtatt tttctagtat tatagcacat ttaaagaagg ctgtgccgtt tttactgcga
     2101 tttttcctga aatgtcaata attagaggtg aattgtccta ttgtcgtcca atctcttgct
     2161 aaaataactc tttataaaag gcaatcgttt cttctaaggt tggcataaat ggatttcctg
     2221 gtgcgcaggc atcaatcaag gcattcttag aaaggtattc aaagtcgaaa tctttttctt
     2281 caataccaag ttcagtcagt ttcttaggaa tacctactgt ctcagaaagc ttctcaatct
     2341 cagcaatcgc ataatcggca cattcttgat ctgatttacc ttctacatga agtcccaagg
     2401 ctttggcaac attgcggaaa gcttctggta cacgtttagc attttcacgt tctataactg
     2461 gtagcaacat ggcacagcac acgccatgtg gcaaattata tactgcaccg agttggtgag
     2521 ccattgagtg aacataacca agaccagcat tattaaagct cataccacca aggaagatgg
     2581 cattgaccat accttcacgc gcttcaatat catgtccatt ttctacagca cgaggaagat
     2641 attccttgat aagctcaata gcaccaatag acagtttctt ggttacatca taagcaccag
     2701 gtgttaccaa agcttcaaca gcgtgagtca gagcatccat accagtagca gcagtcaggc
     2761 ctttaggttt ggaaagcatg agttccgggt cattaacaga gataagagca aggctattct
     2821 tgtcaaccat taccatctta accttgcgtt cttcatcagt aatcacatag ttaatagtga
     2881 tttctgcaga agttcctgcc gttgtattga tagccacaac tggcaagcct tttttagcag
     2941 acttatgaag cccttcataa tcctgtggtt ttccaccatt tgtagccatg atagaaatac
     3001 aactagctgc atcctgtgga gaacccccgc caagactgat gataaagtca cagccatgtt
     3061 cttgcaaagc agctacccca tctgtgacat ttttacaagt agggtttggc tctacatcgc
     3121 taaagatgac atattcgatt ccttctgtat ccagtggttt taaagtctta ggtaaaatat
     3181 cactgccttc gatgtactta tctgtaacca aaagtgcctt tttatagcca agttccttga
     3241 tataaggacc cacttcattt acaacacctt ttccaataag gttgactgcc ggaacataaa
     3301 atgtagccat ataattttcc tcctgcgcct ccgcgctatc tatttaacat cattatatta
     3361 ctttttgtat tcgtttttaa ttatttgtga attgtttaac aaatcttttt aaaaggatag
     3421 gagggacgta agttcacctc ctatataaac aagttttatt tatgtagtgg tcccaacaac
     3481 tgacatgcac aatagtctgc tgattctaga tcttctgttc caaataagga ccaattttta
     3541 ggtctaaaga tatcttcctc aggtacatta tgcatattga caggaattct caacatagaa
     3601 gccaaggtaa tcaagtctgc tccaatgtgt ccatatgtta tggctccgtg attagctccc
     3661 caattattca tgacgtcata gacagacttg aaagcacctt ttcctgtcaa acgtggagca
     3721 aaccaagtag ttggccatcc tggatctgta cgattatcta aagtatggtg aacatcttca
     3781 ggaagttcaa gtgtataacc ttctgcaatt tgtagcactg gaccaacccc ttttagaaga
     3841 ttgagacgta ccattgttac tggcatatcc cccttcgtca agaaacgagt tgagaatcct
     3901 cctccacgga agtattcgcg gtttgctggt gggaagtctg tattttcaag catagcctgc
     3961 acttcacttt cttccaactc ccagaatggt ttcataacag gtttgccatc tcgagtagct
     4021 tgacctgtac catccaatgt acaagaacca gagttgatta gatgtaagaa gccagctgca
     4081 gcacgaccct ctaaagtatg tcccgttaca cgtttaacag cctctgggct ccaataagta
     4141 cgcacatcag caaagatttg tggagtattt gttaatagat aattaaagag catagacaca
     4201 ccatttagtg aatcattctc tgtcgcaaat acaaatggtt ttcgaatacc attccagtca
     4261 aactgagtat tgaggaaagt ttccataaaa tctccatttg gaaaatggtc tgtccactga
     4321 cgttgacctt ggaaaccagc tactaaagca tggtgaccaa ctgcttcttc ctcaaaacca
     4381 agttcagcaa gtcttgggtt accaaccatt aagtcacgtc caatcatgaa catcttaata
     4441 acaaattccc attgtctatc tttttcttca cggcttaaaa caaggtcttc acggttatgg
     4501 tcgaatcctt cttttacgtt ttctttcacc catttgagcg cacgttcgaa ctcttcaggg
     4561 tcgtaaatac tacggtccat acggcgcgtg aactccgtca tatctaccga ttcatttcgc
     4621 attcctaggt attcttggaa gaaatccgga tttacaatag aaccaccaat ccccatcgaa
     4681 acactaccca ttgataggta agcagtgtct ctcatcaagc cagttgcaag agctgcacgc
     4741 gcatagcgta aaagtttttc tttgacatct tctggaatat ctgtgtcact agcttcctga
     4801 acatctcttc cataaatccc aaaggctgga atcccttttt gagcatgtga agctagtaca
     4861 gctgcaagat agacagctcc tgggcgttct gtcccattaa atccccaaat agcatgagga
     4921 atatctggag acatatccat agtttcacta ccataacacc agcatggtgt aactgtaatt
     4981 gttgcgcaaa catttgattt tttaaacaac tcatgggaag ctgcagcctc tggtacacgg
     5041 ccaatagtag atggagaaat cacgcattcc acaggttccc catctggata tttcaatgtg
     5101 cttgaaatca aatctgccac acttttagcc atgttcattg tttgcacttc aagtgattcg
     5161 cgtacacctt gacgacgacc atcaatagtc ggacgaatcc caatacgtgg atgttgaatc
     5221 atactatttt cctcttattt ttctgtttct ttcatactac catctccatt atatttacgg
     5281 tagcctgggt gataattttt aaaattactt ataagtataa acctctacct ctgctaaact
     5341 taaaggaatt cctgcgcttc tcaaagaaat tctaaccttt tttcccttca gtccttttaa
     5401 gtctatggat agattattgc tttctaaact gtcaatatgt tgtgtaaaaa cttcataatc
     5461 atttgaatca tatataataa tatcaaaatt tgataatctc tgaatttcag catctgttct
     5521 attataaata attaactttt ctaattcttc ggtttgtgct aaatctatct cccaccatga
     5581 aggagaatct tctttggtat gagtcactga atgatgacca taatctccgt ttttatttcc
     5641 atcaactgct agacgagaaa aaccattgta atccgttgaa ctctgtcgag tttcttttgt
     5701 taatgcaata ttagaaagtt tcttcggagt tctctttgaa ccatagactt caacttccgc
     5761 taaacttaac ggaatagctg ctgattttaa ttctattttg actagcttag cccctacaga
     5821 aggtactttt aaagataaca aaccatcaac tactttatca aaatgtcttc taaaaacttc
     5881 ttctcctgat gaagatagaa aaataacatc aaaattagat aaacgctgag gttcggcatc
     5941 tgttctatta taaatatcaa ctttagaaac cgtaaactct tctcccagat cgacctgcca
     6001 ccaagcgtta gaatctgcct tagtatgagt caccgaatga tgtccgtaat ctccatcttt
     6061 attaccatca actgctaaag cagctacacc ctcataattg gttgaacttt gagtagctac
     6121 cttatttgta gagactactt tatcttctgt tattttatct atatcctctt cagattgctt
     6181 accatctgat tctctaaaaa cctctacttc tgctaaactc aaaggcactc cactcgttag
     6241 taatttaact ttaatatacc ttgctccttt ttctttgaaa tctagactaa cagattcacc
     6301 cgacaaatta ttaacatgtt tcttagcaac ttcgtttcta ttattgtcat atagaatcac
     6361 atcaaaatta gatagacgtt gagtctcagc atctgtgcga ttataaattt taacaagccc
     6421 aactttatcc atttttttca aatcgacttc ccaccaagag ggattatctg ccctagtgtg
     6481 tgttaccgaa ccagagttaa aattaccatt tctgttacca tccacagctc tattagggtc
     6541 tccattgtaa tctacagaac tttgtgttgt tggttttgca tatgctatat tcaaattacc
     6601 atcattaaat ttatctggtg ttgggaaaga gactggacct gtcccctcag tatttataga
     6661 catctctacc attccattat gattaaccgt aatggtataa acatgggtat tctcatccca
     6721 attttctgta taagtataat gatttttatc gccacttata tttatctgag gtttatgacc
     6781 agtatgccct tttaatgtaa gtgttgttgt cctaaagtca ttatctacag gattgatgga
     6841 ataatttttg cttatccagt tcgctaactc taattcttct ttcttccaac tttttgatgc
     6901 atcaaaattt cctgataatg cccacatagc tgtcttatct gtcctgtaat tattcaataa
     6961 aatatgtaaa ttatttggat tttctttaac aacagcgtaa gtatgtggcg tcaaatctaa
     7021 cgataacgac tttgtattat tagtatacat aggcaacatt acttgctgat ttttattgat
     7081 attagcatta ctattataaa tataccagga attacctaca cgctgagcat acccatctcc
     7141 ttcataaagt tttggataaa gcgagtttaa atagttgact ttactagaca attcctcact
     7201 atttttagtc aaaatttttg cattagggaa tatagatgaa atcttttcct tatcaatttt
     7261 ctcatgtatt acaggaagaa tatgatatct cccattatta tataaaggca ttgtttcatc
     7321 attcgaataa agtccttgat aaaatccgtt taatgaacta atcctacctt ctccattcca
     7381 aaatacagct tttgttctat ttacaacttc ttccttactt ggagctggat tttgtatagc
     7441 atgtctaaag aaaggaataa tacctttagt aaatgctgga gttggtacat catttgtcat
     7501 aaatgtatac gcggcacatt cgaaattata aactgtgcct cccccagtat atacattcat
     7561 catttccata gcaatcattg attctggttc cgatgcatag gatctcatat ccctagctct
     7621 tccagtttca aatgtgtttg tataatgttt ttcccaccat ttccatgtat ctgttgatga
     7681 gccccagtta tcacataagc cactcaacca aaatccacta acgatagaat ctgtacccgc
     7741 atcatctctt attggcgtat ttttagttgc caacaccaaa tttttatgat atttttgact
     7801 cgcttcaaag aatgtcggat cattcataat agtttcccag aaccattttt catgatcatg
     7861 ccagataaaa tgcgctccat atttggcaca aacttccaaa tatttagcac tatgcggagc
     7921 taactggtta ttgtaaatcc aataattctc aatatttaaa acacctttta acacactata
     7981 cttttggaat tgttcatcta accactctgg aggaactgta ttacgctctc cagccgacat
     8041 aataaccaag aaaactggaa tgtttagact ttgagcttcc tccaacatct tgacatacca
     8101 ttctctcaat tctttcgtat ctcgtggaat acaacttgtt ggtttacaga cttttgcagg
     8161 atgaagttca atcgctgcat atggctttac atcttcagga atagcttccc atgctccctt
     8221 caatgtgttc ccccaccata atccgttacc attatcattg ccatacaacg gagaaagcaa
     8281 caatggcgat tcattattaa tcgtcgttct catttgaaca cgattatcag ccttaactcc
     8341 tgctaacaat ccaaaacata acatcccaat acatacaaac aatattgtga ttaattttct
     8401 ttttattttt tctttattca tgacttatac ctaaaaattt aactcaataa ttttttctga
     8461 attaaatacc aattcaatat tttgtacatc agtatttttg ccatatattc ttactcttac
     8521 tttttgatct ttgtttcctc cttccaattt taggaatgtt atatccccat ttttccaagc
     8581 aaacgatacc ttatatcctc ctcttactct gaaacctttc acttctcctt ctgaccaagc
     8641 agaaggtaaa gctggaatta gtgataacca attatgatgg ctctgtacta ataattcaca
     8701 aattccactc accaaaccta aattaccatc aatttgaaat ggtggatggt caagaaataa
     8761 attgccaaga gtcgcattat ttaacaaacc attaatctgg ttataagcag gttcaccttg
     8821 atatagtctc gcaaaaaaat gaatcagcca tgcagcactc caacctgttt gtgtactagc
     8881 atgcaaacca cttactaacc aattattaat cgcttgctcc ctctcctgtg aagataaaaa
     8941 attagcgttt gataatctcc tattgatagt gattttagct gcttctgcta attccggagt
     9001 tttatgaata tcaatctcat tataaggata aagcccaaat agaggtgaaa tgtgtctatg
     9061 cccaggctct acttcttcat aatcttctaa ccattcttgg atttgcccat tactacctat
     9121 ttttgtttta ggtagtttct tttttaactc cttcacacga ctaataaaat ccgaattgtc
     9181 tcctaattgt tttgcaatgc caatgcatga atcacaaaaa tatcttagaa tttgattatc
     9241 aattgtagat gatagacaag catttccttc aataccattt tttaagcgat atttattttc
     9301 cggtgagaca cttggacctg tcatcaagta gccatccacc tcaaataaat aatcttcaaa
     9361 gaaaagaaat gcttctttta tcatttcaaa atgttccgta agaatacgct catcttggaa
     9421 atataaatag tgttcccaaa tatgagtaca taaccatgga atagttaata cccaaattgc
     9481 agcccccatg gcatgagatt ggggagccgt atcgccaaaa ccatccgtat tatgatgtgc
     9541 tgtaaaacct ctagctccat acattttctt agcggttagt cttcccggtt ctctcattct
     9601 ttcgagcata tcaaataatg gatattctac ttctggtaaa tcacatggac ctaccatcca
     9661 ataattcatt tgagtattaa tattaatcgt atatttagaa ccccaaattg gatttaattc
     9721 atcacaccat attccttgaa gattggcagg taaaccattc ggttgactag acgatattaa
     9781 cagatatctt ccataatgaa ataacaagtt agtcaagtag ttactatact ttttagtgtt
     9841 ttcaagaagt agattcgttg gaatgctaag acaatcttta ctatagtcta gtttaaaatc
     9901 aactctatta aattgctcct gatatttttt tacatgttca tctttttctg taaagtaatc
     9961 aatactacta aattctccct gaagagaaga aatatctata tttccccaat aatccgtcat
    10021 tgatttgaga taaagaaata cctctgtagc attccgaata actattgtct ctcccaatac
    10081 acttacttca ccatccgtaa ccttagaatg acatactact ttaaactgaa caccttttct
    10141 acctccagca gaggccgaca ttaaaattgt acttgaatcc agtttagata cttcatcatt
    10201 aaaccgttta tttctaccca aattaatgtt taaatttaat gtgttttgaa ctgatgacac
    10261 tatacggcaa cataaaatat tcttattaaa actcgtaaaa tattctcttt ttatttgtaa
    10321 attacaacta ttaggctcaa acacaacatt agaaatagct gtatctaaat ctagctctct
    10381 ttcatacaat gaaagagcac aagactgaat atctatatgc tcaatgtaaa gttccccaag
    10441 taattcatag tggctttgat ctcttggggt agcaaacatt gttaacttta tcaattcttc
    10501 ggctttctga atttctccat ctaaaagata ttcccgaatt tttttaagat gcaatagtga
    10561 gtctggatta tttctatctg actttcctct ataccaaata gtctcatcgt ttagttgaat
    10621 acattctttt gtagctgaac cataaatcat accacctaaa tgaccgttcc caatcggcaa
    10681 ggcttcattc caatttgagg cagctttctt ataccataat ttcactcttt acctctcttc
    10741 caaaatggag gtgatatccc aaataccacc tccacacttt ctactacttc aaaattccta
    10801 aaacaactag gataactcca atagccataa cactaaagat tagtacaacc ggattctttc
    10861 cttttctaat taatttacac attaataaag tcaaagccat agggataaat cctggaatta
    10921 attttgtaat catttcttga acagaaataa caagttcccc ctgcttaaat tctaatccaa
    10981 aattaatacc aaccattgat ggaatcaaac cacccactag tactagccct aatgctgtcg
    11041 ccatactcgt aacgcgattc aatgttcctt ttgtattatt ttcttggata agactagaac
    11101 ccttagtata cccatatttc aaaccgaaat atttaacagg aatattaata atattaaaca
    11161 atattaaggc gataaagata cctaaagcac cgccgtcttt agaataagac gcaccaatac
    11221 taaaacagat aggaactagt gtcagccaga acaaactatc tcctagacca gccagtggcc
    11281 ccatcaagcc aactttaata ccagtaattg aagctgcacc ttcatttcct tcttgttctt
    11341 ccatagcgga agtaactcca agaataaatg gtgccgctgt ttgatgagta ttgaaaaact
    11401 caaggtgacg tttcattgca ggagaagctg aatctttgtc tgggtatagt tttttcaata
    11461 ctggaagaat tgtatataga aaacctaggt tctgcattct ctcatagttc catgaagaac
    11521 catacataaa acttcgtttg aaaacttttt taagttcaac tttagttact ttattattca
    11581 tcatagatca agatcctcct caacatcact acttgaagtt gtttcttgtg gtttacttcc
    11641 aatcatatcg tagaagtatg ctaccgcaat accaactagt gagatcccaa tggttgacat
    11701 tcctccatac acagaacaaa tgaatccaat caacaagaat acccacatat ttttcttgag
    11761 catcatattt aaaagcatac caaaaccaat tgaaggtagt aaagcacctg cagagttaag
    11821 tccctgcata acgattggtg gaaccttagc aaacaagtct gccacatatt cccctccaag
    11881 cataatagct aggaaaattg gtacaatttt aaataacgtg attaaaacca aacttgaata
    11941 atgataaaaa ccgatctttg aataatctcc gtttttagca tcattatcag cttttttcac
    12001 aaaataaaca tctaaagttt ttgcaagaac atccaactgt tgggtagcaa ctgcaattgg
    12061 aactgctata gcgataccag cagtttctcc ttgaccagat aaaataccaa atgcagtacc
    12121 aataatcgca cctgaaatag tatctggtgg agtgtaacct ccaatacctg ttacaccgag
    12181 ccaagttaat tcaagagctg aaccaataag aacaccttgg gtgaaatctc ctaagactaa
    12241 gccaaccatt gcacttgtaa ctaacggacg gtgaatcata aataacggtc cattatagtc
    12301 aatagtggca ataatcccaa ctaatgtcac cagtaatgct tgtgtgaata gcatatactg
    12361 tacctcctta aattattttt ataaaaattt ttccaacatt gtaactttat cattaggaac
    12421 catttgaatt tcaactctcg ttcccttatc aactattttc ttaaaataat atttgtcttc
    12481 ttcacctaga gctacacctc ccgttacctt ttcattttcc tccgttttac tcatctgtcc
    12541 tacattgagg tactccaatt ttaaatttcc ttcaatagaa tcatacacat cttttggatt
    12601 tgtatatatc agcattgttc tcttttttat tggcacagag tttaaaactt ccacaaaccg
    12661 ttttacacta aagaaaacaa tttttaaacc taccggtgca gaaatcttta aaatagattg
    12721 tcgtgtttta tcttctgaga tgcgatcatt aacaatgata acttgctcaa tatcatactt
    12781 ttttagccac gtagtgacaa cttgaccatg taccagacgg tcatcaatac gaacaaactc
    12841 tatagacata aatcctcttc ctcctctacg ttaagttgtt gtttaacatt aaacaaacta
    12901 ttttgagcat tttgaacaat ttcttctaaa ttgatttttg aatcataact tgatattaat
    12961 tccactagga gagggatatt aaaccctgtt acaatatcaa ctgaatccaa atttaaaaac
    13021 cgtgacaaag ccacattatt aggacttcct ccaatcaagt cagtcaaaac gataacctct
    13081 tgatttgagt ctaacagttc atttacttga tttttaaaat aatgttcaaa ctctacaata
    13141 ttctctccag gcatcaaacc taatgtccta actctatcat ttgttgtctc accaacaaac
    13201 atttctgcgg tcatgagagc tccgctagca aaattaccat gactggcaac taaaattcct
    13261 ctattaaaca ttttctcttt caacaactac tcctttctta aggataatat tagcataaag
    13321 tgaagtttct cctgtagcaa caatagcata agctttctta ctacgttcat aaaagtcttc
    13381 tcttctaaga taagtaatcg ttttaagatc tgtaccatga ccttcaatca tctgtctata
    13441 ggtaccccat atcttaggaa tatcatcacc cgaaacaacg ttcataaact gaattgaact
    13501 atcgacgtaa ctatctaatg gcattaaata cagaatggaa tctaataatt ctggaatatt
    13561 tacgccatca caacgaatta gcttatttgc acatgaggca gaaggataat tcgcgtcagc
    13621 taatactatt tcatctccat gtcccatttc cattaaagtc ttcaataaat ctggagaaat
    13681 atttttcggt atatgtttta acattaatat cctccctatt ttctctgacc gtatgtttta
    13741 aatttttctg ccattaaatc catctcctca tcaggaagaa ctactggctc tccaaaattc
    13801 ttagctaaac aataaatttt tgcacaatat tcaacttctt caacaatatt aaatgcattc
    13861 aataaatttt gtgcacctgc taaaattcca tgattcgcta gtaaaactgc tctacgacct
    13921 tccattgctt tagctgcatt cacagccaat tctttcgtac catatgttgc atactcagct
    13981 acccgaacat ctttccctgc cactgcaatc atataatgac tagccggaag tggttctctg
    14041 agacaagcta atactgttgc ataagttgta tgagcatgga taattgcatc gatatcatca
    14101 cgagtttgat attgaatcaa atgcatatac cattcgctag atggcaagcg ttctccctct
    14161 acaacatttc cattaatatc catcactaca atatcggatt ctttgatttc aaagaaatca
    14221 atacccgacg gggtaattgc catcaattgt ttttcacgat cgaaaacact gagattccca
    14281 cctgttcctt tcgtcaaatc tgtttctact agcttcttgc catacttaat taattcttgt
    14341 tttacatctg acattcttgt cctcctcata tcaaaacact aaatattttt caattcacca
    14401 attatccccc cttcttgtac aaaacttaag aagggggagg gaggcaaatt tacaaactct
    14461 caatcttttt aattcttttg ggagtaataa ctctctaatt gtagaaactc ctcaataaca
    14521 tggtgagctt cttccatccc ttttaattgt cccatagcta tgagctgcac aacaatattc
    14581 cccacagctg taccctcagt cgaacctgta agaacctctt taccagttct atcagcaatc
    14641 atttggttaa agtaactggc tctagcacct cctccaatca catatatcct cttataaacc
    14701 ttatgagtta gttcttctag taactctatc gctttcctat acgtttcagc taggctttca
    14761 taaacaatct taaatagttg tccatctgtc cactctctag tttcatgatg ataagctaga
    14821 tattctgtca aagtcttgtg catatcagat tctgttgcaa attcagttga ttcagtatca
    14881 atcagaggaa gattttcttt ttctttctcc accattgtcc taatatcatc aaaagaatag
    14941 gctttccctc ttcgttcaaa tgaacgtctt agttcctcta tgatccacaa ccctgtacaa
    15001 ttcttcagaa atgtaatcac tccatcttta ccgacttcat ttgtaaatcc ataactgaag
    15061 gattcggtag taagaatcgg tgaagtaagt tccactccaa ccaaagacca agtacctgat
    15121 gaaataaata aactaccttc tgtcttaggt actgagacaa tcgcacttgc tgtatcatga
    15181 ctacaaacat tcacaacagg aatatcgcct aaaccatact ccttttttat ccttccaaga
    15241 acatttccct ctgaaacaat ttcaggaagt aaagatgaat ccaattcaaa tagttttaag
    15301 atattctgat tccaattttg actcctagga tcaaataatt gagttgttga agcaatgctt
    15361 ttttctgtag caaacttacc tgtcaagaga taattaaaca aatctggcat taaaagaatc
    15421 ttattggtct tatagaaaga gtcaggagat tcttgacgtg ccttaaagag ttgaaacaag
    15481 gtatttatct ccataatctg atttcctgtc tctgaataca gtttttctaa ttcagtcatt
    15541 tcagatattt cctttaacac tccctttgtt ctttcatcac gataatgaac aggttgtaat
    15601 aacagcttac cttcattatc aatcagtcca aaatcaactc cccatgtgtc aataccgata
    15661 gataaaatct tgtaactagt attagccaag cggatacttt caagaatttt agctagtaga
    15721 aagtcaatat cccaagataa atgccctttt actctaatag gtagattaga aaagcgattt
    15781 atttcttcca ttactagttt attttcagaa aggtaaccaa cgattgctct tccagaagtc
    15841 gcacctaaat ctattgctaa aaccgcttcc atttttcttc tctcttcgtc cttgattaac
    15901 tttattataa tccccacgtt ttattttttc aatatgtgaa cgcttacttg aataatgttc
    15961 aaatgttaac ccaatattca taaaaataat tcaaattcct ccaatttgtg ctataatata
    16021 gttgaaatga taaattagga ggagcttatg ataaaagatg aacgtgtact tgaattgatt
    16081 gaaattatta aaaagaaaaa aagaattgcc gtaaaagagc tggcagaaat cactttctcc
    16141 agcacaagta ccttacgtcg tgatttaatt ttcttagaaa atcaaggtct tatcaaaaga
    16201 aagcacggat acgtgaccct gtcctctatg aacacaattg aactttctca tcaaatacgt
    16261 gaaggagaaa gtactaggca aaaaagacta atcgctagtc tcgctaaaga ctttattcgg
    16321 tctggtatgt gtatctatct ggattccagt acgactgtct acgaactttg tccctatctt
    16381 tctgaacttg ataatttgat tatttttaca aatggtttac atactgcaca aaccctatct
    16441 gaaactgtta aagatagctc caaaatcttt atcacatctg gcgaggtcaa acatcaatcc
    16501 tgttccgtgg tcaactatga taaggaaaat tctttattag atcattttaa tatcgattta
    16561 gcattttgtt cagcaagagg tattgatgac caatatgttt atgaagcttc tctcagccaa
    16621 gctatttcaa aaaagaatat tattgacaaa gcccatgaaa ccatcttact gattgatagc
    16681 tctaaatttt acaagactgg attttttaaa attaatcccc tatccaaata cacaaccttt
    16741 atttctgaca ccgtgccaga tcaaaaatta ttagacgcag tcgaattatt tgatggagaa
    16801 tgggtttctg atattcaatg aaaatcaaat aggaagctag tctcagcttg ctcaaagtac
    16861 agatttgaaa actgatgaag tcaagtcccc catggacacg gcaagacgaa gctgacatga
    16921 tgtgaagaga ttttcgaaga atactcgttc tgtctgctga ctgatttaca aatattcacc
    16981 acatgacatt attttcttag caaccctaaa aacagaggct ggacaaaaac tcaatttcaa
    17041 gagaaatggg gcaaatattc ccacaataaa acgcatagta tcaagttttt gcacacctga
    17101 tattatgcgt ttttctgatt ttaaagactt tgtcgcctag accctatatt tcaagaatta
    17161 agtgaccttg atgatctaaa tgtattgtgg tatactattt ttgtcatcgg ttaccgatta
    17221 cgttcctatg ggttcgtgag aggaggtgaa actaatgagc cttatcatag agctcgctct
    17281 tactattata gcggatgtta tagctggaat tatcttgtat ttcgtctgca gatggctaga
    17341 tagtaaggag tagaccgaac gactagccta taaacacccg ttaaatctct aagatacgtc
    17401 aaaaaagccc ttaactacgg ccatagttag gggctttggt gttctaatga accttatcaa
    17461 ctaactacat tttagcatat aatctacaat agttcaagca ttaagtgagg ttgatgatct
    17521 aaatttagtg tggtatacta tttttgtcat cggttaccga ttacgttcct atgggttcgt
    17581 gagaggaggt gaaactaatg agccttatac cagagcttgc tcttactatt atagcagatg
    17641 ttatagctgg aattatcttg tatttcgtct gcaaatggct agatggtaag aagtagaccg
    17701 actgactagc ctataaacac ccgttaaatc actaagatac gtcaaaaaag cccttaacta
    17761 tggcactagt taggggcttt ggtgttctaa tgaaccttat acactaacta cattctagca
    17821 tataagtcct aatatttcaa gagttttatt tattttttca ggttccctta gttctgaaag
    17881 gtctataatg aagttagcca tctagtatca aaaaaccgac tagctcttat gaactagtcg
    17941 atttctcatc aatgcgccaa catttcttgg gcgatttctt ggccagatag gttatctggg
    18001 tagtaggttg gccagttgtc catttcttca aagagggctt cttggcttgt gcctccaaag
    18061 aagatatgga aatgttctgc cttaactggg gcgatattgt ggtcactaaa ctgaacatac
    18121 ttgaattgtc cagcgtcagc atctgtggct tcaaagagga aacgcacgcc acgattgcct
    18181 ttcttgtaag tcaaaatttt cttaccgaca tacttgtaag tgtatttctt gctttgtcca
    18241 ccttgaacaa attccatagt attatcagta atgttaatct tagtcacatc tgtctgatag
    18301 ccttttgtat agtaagcctt gtactcagcc tgggtcatct taccagtcaa cttagccttg
    18361 tagtcaaaga cttggtcaaa cgtgccgtct tcaaggaaag gataaactga ttgccagtta
    18421 cctgcatagt cactcaaggt gcggtccttg acagctgcat cctcgaagta accattttgg
    18481 actgtcttgg tatcctctgc cttttcaggc tcgattgctg ggccttcttg gtctgttgtt
    18541 tgtttcaaag ccttgaggtt tttctccatg atagagatgt aattttctcc agccttggtg
    18601 tcctcttctg tcagactttc taaaggattg aggacatcag ttttgacacc tgcttctttt
    18661 gaaagtgtgt tagcaagggc ttgtgaggca ttttcttcaa aatagatata ggcgatttta
    18721 tttttcttga catactctgt caattctgcc aagcgagcag ctgatggctc tgcatctgga
    18781 gaaagtcctg agattgcgac ttgtttgagt ccatagtcca aggcaagata gttaaaggct
    18841 gcgtgttgag tcacaaagct cttttgtttt gcttgagaca agccttctac gtaagcctta
    18901 tccaaggctt gcaatttttc gatataggca gctgcattct tctcaaaggt ctctttttta
    18961 tcaggataat ctgctgacaa gctgtcgcgg atgtgctcta ctagtttaat ggcacgaact
    19021 ggtgataacc aaacatgggg gtcaaactca tggtgatgac cttcttctcc atggtcatgg
    19081 tctccctctt cttcctcgcc acctggcaag agcaacatat cgcctgtcgc cttgatggtt
    19141 ttcacttttt tcttatccaa ggtatctagc aatttaggta cccatgtttc catgttttca
    19201 ttttcataaa cgaaggtatc tgcatcttgg attttggtaa ctgccttggc agatggttcg
    19261 tattcatgag gttctgtccc agcaccgatt aggagttcta cattagccgt atctcctgcg
    19321 acttgcttgg taaattcata gacagggtaa aaggttgtca cgatattgag tttaccatct
    19381 gcctgttttt gattggaaca agccactaaa aacaaggcac atagactggc tagtaataag
    19441 ctaatttttt tcacgttcgt ctcctatttg ataaaacgtc ttactaaact gattagtata
    19501 aagacagtta caaaaataat ggtaatactt gcacttgcag gtgtttctgc atagtaggaa
    19561 atgtaaagtc ctgctaccat tcccaaaaag ccaatcgcac tggcaagcag cataaccgat
    19621 ttaaagtttt tccccagacg cagggcaata ctagctggca agaccataat ggtcgatacc
    19681 agaagagctc ctgctgcagg aatcataagg gcaatagcca cccctgtcac catgttaaaa
    19741 agaatggaca tggtacgaac tggcaagcca tccacaaagg ccgtatcttc atcaaaagtt
    19801 aagatataca taggacgaag aaagagaaag gtcaaaatca aaacaaccgc cgcaatgaca
    19861 aagagggaaa tgacctgttc ttcactgata gtcacgatcg aaccaaagag atattggtcc
    19921 aaactcattg aactcgagct tttacccttg ctcatgacaa tcagagaaac agccagacct
    19981 gttgacatga ggatagctgt cccgatttcc ataaagctct tgtaaaccgt acggagatac
    20041 tccagaaaga ccgccgcaat caagacaatg gcaatagtag aaacagttgg agaaatcccc
    20101 aaaaccagac caaaggctac acctgaaagt gagacgtggc taagggtatc actcatcaaa
    20161 ctctgacgac gcaagatgag gaaggttccc aataccggtg agaaaagact catagcaata
    20221 accgccaaaa aggcgcgttg tataaagtcg taagataata aactaagcat ggctcacctc
    20281 ctggccattc tcatgaacgt tgaaacaacg ccatggcgag tcttggttac ggactagatg
    20341 aatattgcga tccgcataat ccttaacttc ttcagggtca tgggtaatca tcaaaacagc
    20401 cttgccatga tgatgggcgc tgtggtgcat gagttcgtaa aattcatttt tacttcctgc
    20461 atccatcccc gttgtcggct cgtctaggat aaacacatca gggtcagaag caaacatacg
    20521 cgcaattacc gctcgctgct tttgtccccc agatagagac cccaagcgtt tgtctcgatg
    20581 ttcccacatg ccaactgagt ccagactggc cttgatatgc tcctcatcat gagcattcaa
    20641 acgacggaac cagccttttc tcggatagcg acccgacttg acaaattcat agaccgtact
    20701 tggaaaacca gcattaaaac tggcaatttg ttgaggaaga taggctattc tcaatttctt
    20761 accttgcgta tttgtctttg aaatagccac ctttccaatg cgtggttgca gaattccaag
    20821 actggccttg atgagcgtcg tcttagccgc tccattttcc ccagtcaagg taacaaattc
    20881 cccactatca acactataat tgatatgttc aagaacaggc tccttatcat aatagaagga
    20941 caaatcctct accgtaatat atctcattat ttgatttctc ctactaaagc agtcaaaaac
    21001 cgctgaatca ctttttgttc atttggagta aactgagtcg ccacttgttc ataggttaaa
    21061 agtgtatgct catggtgatg gtggtgctcc tcagcgattg gacgagccaa gtcagtcaac
    21121 tgataaaaaa tcacacgcgc atctttagaa tctttagatg tttccaacat cccttccttg
    21181 accaaagact taatggcctt ggtaactgcc gcctgactga cattgagacg acgggccaat
    21241 tctgaatttg ttaaagattc ctctgacaag agcataagga tatgctcctg agtattggtc
    21301 agggccacct cgctagtgca atgacctatt aggatttcat gctgattttc cgcctgcaaa
    21361 atcacctcat tcaaaaaagc attgatatcc tttgctagct gtctcatatc tgactccttt
    21421 ccttttagac ttctcttttt taagagaaaa atactattct ttgacatttt gtttaccagt
    21481 taattatatc acaagcaaaa aaagagtcaa gaaaaaacgt gaaaactagt ttcattcttg
    21541 aactcttcta tattatatta tctattgaaa ttctttgaca tctccatcat aagtcgccca
    21601 atctttgctg aaaaagcgct cattcagatg gtaagtcgga gctggtgtgg gattggatag
    21661 gaaaggatca actgccttgt caaaagccaa ccaacccaac caaccaaggt gaatggtgtc
    21721 cttcataaag aaaggctccc caccgtcctt agaaaaatct gctatattgg taaaaccttg
    21781 actttctaac tggtagcgaa tcttctgcac cgtttgttgg tacatatcct ctcgtagacc
    21841 agcatagtcc atccattttt tattaacagg tggaatgata aaaatcgggt ttaccttaga
    21901 tttagaaaac tgtgttaaaa ccaactgcaa gtcattatac tctggcgact tgagataggt
    21961 aaagcttttc tgagaatcct ttaatttctt caaatccttc ttgatctgct cattatagaa
    22021 ataattttcc attcccatct cattattgga agtatttttt tcagcatctg ctttgacaac
    22081 atcttctatt gcttgataag aaaactggtc tggcaagatt tttaaatact tagctacatg
    22141 cttatcgtag ttaacatagc ctctaaccga aaactgacca aaaaaggagg cttggcgttc
    22201 attaaaacga gccaataatt caatcatttc attgtctgct gtcgacaatt ctcctttact
    22261 tgccaacttc tgaaccaggt ccttcatagc tacgtttggg aactgttgca gtaagcgagt
    22321 cgctgcatat tgactagcct gatccccaga ttgatgtttc agaaaactag tcaactggtc
    22381 tccattaaaa tactgctgga aggctgctgg atcatagcca tttttactga accactgagg
    22441 tgagataaca tacacaactt gtttattctc cagctgtggt aacatctgtt gcattccaaa
    22501 atattggtta agcgatgcag ctcccccctg tcctaaaaga taaggacggt aggaacgatt
    22561 gtatttctca gctaataccg caggatgagc accgtcaaaa cgaagccatt cactagagcc
    22621 aaagaaggga ataaaacgca catttggatc agatagtgct ctgacttttt gacttcgctc
    22681 cttaaaacta tcgatagtag tagccactgc tgaacgcttt tcagctccta gattatgatg
    22741 catctcagta ggataaaaga aaatgagcag aaaaaccaac aaaccagcga tcaagaccgg
    22801 tccgaagatc atccataagc gtttaagcat tttgtagctc cacaatacca gctatgattt
    22861 tattagctgt attccagtcg tcacgaccaa actctgttac agggacacga atgtcaaaac
    22921 ggttctcaat ctccacaatc aactcaaccg ttcccatact atccaagaca cctgcatcaa
    22981 aaagatcttc atccatcatg tcagaaacat cttccataaa caactcatca ataatttcaa
    23041 taacttctga tttgatatcc atattttatt tccttttatt ttttaaacca tagattattc
    23101 aagaatccag aaaagattaa gaatgacaac atgacaacat ggaaagtgac aaccatgcca
    23161 agaaactgaa tccagcgatt ctcaggtagg gcagccttcc ctgctttttt ccgttcctta
    23221 ttgagcgttt ttttcttgcg aacccaggca tcattgatga ccaagcctag tccatgaaag
    23281 agtccatagg cgatatagta ccaggtcacg ccatgccaaa atcccataat cagcatattt
    23341 acaatgtagg ccatgcttga ggttacatta cgatttttaa agactttctt tctggttaac
    23401 accatcacca ttcgcataaa gacaaagtca cggaaccaga aggacagact catatgccag
    23461 cgattccaaa actcctttaa atcccttgat aaaaagggct tgttaaagtt gatagggcta
    23521 cggattccca tcaagtttga gatggccaaa gcaaacatag aataacctgc aaagtcaaag
    23581 aagagctcca gaccaaaagt atacataact gccaaggcat agagattaaa gaagccacct
    23641 gactgcaagg ctaaattctt cagaggaggg agtaaggtct ctcctaaaac atgagctagg
    23701 ataaacttat acaaaaagcc ccgcatgata tagcggacag attcatccag catatccatc
    23761 aactcatctc gctcaggaat ggcctgataa ttttcattaa atcgcttaaa gcgatcgatt
    23821 ggaccactcg agaaagttgg catgaagaga aggaaacgga ggaattccca gagggtaaaa
    23881 tccttaatca ctccatctct cagctcgatg ataatcccaa ccgaacgaaa ggtcaggtaa
    23941 gaaattccca agaacccaag caaagactgc gttccattga tagctggttg caccttgaca
    24001 aagataatcg gaagtaggga cagaaaacta actaagtaga agacccactt gccatccttg
    24061 ctttttcgat aatgcttgta gaaaagcagg agcaatattt cccagcaaag gtaaataccc
    24121 aaggcagcta gttgattggt ctttccaccc accaacatgg tgacaataaa gaagagactt
    24181 accaacactt cataccaggc aaagcgtttc ttgaaaaaga gacctataaa gatgggcaag
    24241 gttgcagcaa tcacataaac aaaatactga ggattgccat atggctctaa atgaggaagc
    24301 tgttgaaaaa actccatcat ctcttattca cctcgttaat caatcctttg atgtcaatct
    24361 ttccatttgg agttagtggc aaactgtctc ggtaaaggaa tttagatggc atcatatagg
    24421 acatcatgat gtctgtcaga tcttccttta tggccttggt aatatcgata tctcgctcaa
    24481 actgctcacg aacaccgtct tttaagatga cataagccaa tagattttgt accttgtggt
    24541 ccttgttata gcgcggtact gcgacagcag attcgataaa gcgagacttg ttgaggtttt
    24601 gagagacatc ttctaactca atgcggtaac cgttaaactt aatctggaag tccatgcgtc
    24661 cgccgtagag aagcaagccc tcatctgtca tggttcccac atcgcctgtg tgataggctg
    24721 gcagatcttc aaactcaaag aaggcttctg ctgttttttc aggattgttc atataacctt
    24781 ttgaaacagc tggcccagaa acaatgattt ctccctgctc accatttggc agtttatttc
    24841 cttcctcgtc aatgataaag gttggagaat cagccttggt atagccgatt ggtaggcgtt
    24901 tgagagtcgc taacatctcg tctgtcacgg caactgctga cagagctact gtcgcttctg
    24961 ttgggccgta agcattgatg atacgggcat ttgggaaacg ctcgcgcagt ttttgagctg
    25021 ttttgaccgt caattcttca ccatcaaagt agaaatgcgt gattccaggc attttctcac
    25081 tgttgaagta ttcagacaac atggccatat ctgcaaagga tggtgttgat gtccagatag
    25141 cgattggcaa tgaaaagata gccgcaaaga gttgcttaaa gtcctgagtg atgactgaag
    25201 gaagagtgaa aagcgtacca ccaagtgcca aggtcggtgc ccaatacatg acagacaagt
    25261 caaaagaata aggtggctgt gccagcattt gcggacgact cggtgtcgca aattccttat
    25321 ccgtaatcat ccagtttgta aagctgagga gattatcatg tgaaatctgc actcccttag
    25381 gcttaccagt cgtaccagaa gtaaagataa tgtagtaatt atcatctccc ttgactggat
    25441 gcgtgatttc atagttattc ccttgggcaa aggcttcttg aacctgagct agatttatca
    25501 ttggtgtaga aacctgctcc aagggaaagg ctgaaatggc aataatcaag cttggctctg
    25561 ctacttctaa aatagctgaa actcgctcta aggccgaatg gctatcaatt ggaatgtagg
    25621 catgacctga cttagtcagc gctacaaagg ttgccaacat ttcatattct tggccaccaa
    25681 aaacaaccac aggagacttc tcaggcaagc ctagttggtc aatgactgca gccaaactat
    25741 ccgaatcagc ctttaaatcg ccataagtgt gttcctgccc caaaacatta tagacaggat
    25801 agctaggctg tgtctgagca aaatgctcaa tggtttcaat catatctgct attggtttat
    25861 ttgacacaat agggattctc cttcaagtta aaattcatta tagataaagc ttccttgacc
    25921 ctgaccaaga tagctaaaga agtaaagcag ccctagaaag ataagaaaat acaaggctgt
    25981 ccgaccaaga aagaggtaca attcttttct ctgtttcatc aagaaaaacc attcatttct
    26041 gtaatttttc gctaaaataa gagtgattct tactagctta tttttctacc attgtaccac
    26101 tttatatagt atcttttcaa ttgtttaccg tatgtttcca atagatttca gcttatttta
    26161 aggattatgc ggtttttcta tatctatttc aaaagaaaaa agcctgagat tttcatctca
    26221 agctctagca ataatagtta ttttctaaac taaaaaagtg agaatacaag cacggccaag
    26281 gctgctccga taacagggcc tacaacagga atccaagcgt aagaccagtc tccgtctccc
    26341 ttgtttggaa ttggcaagat gctgtgcatg atacgaggtc caaggtcacg agctgggttc
    26401 aaggcataac ctgttgtccc acctagtgat agaccgatac cgacaatcaa agttcccact
    26461 gcaaaggttc cgatacctgc ctgaaaatcg taaagaccca aagcaaagat tgtcaacacc
    26521 aaaacaaagg ttccaaggat ttcgctaatc aagtttgata cagtatcctt gatggctggt
    26581 ccagtactga aggttgccag gatattgcct gcattttctt ctacctcata atgcggtttg
    26641 aattgcaacc aaaccaaaat ctgacccagc atggcccctg cgaactgggc taagatataa
    26701 ggcaaaacgg aagcccaagg caaaccacct tttaaggcca caccgatggt cacagctggg
    26761 tttaaatgag ctggactgag cttgccagat acaaatactg caaccgcaac tgcaatcccc
    26821 caacccatag taatcacaat ccaacctgca ctgttgctct tggtttttgg aagaaccaca
    26881 cctgcaacaa caccatttcc tagaagaatc aggattaaag tccctaaaaa ttctccaaat
    26941 aattcactca tcatttttct gtctccatta aaaaaggggc gggcgacaag gattgctacc
    27001 ctccacctct tttatttttt cttaattttt taattctgct aagtcgtttt gagcaagagc
    27061 tgcttttaca tcagcacggt aagttgcttt ttcttcttct gtccagtcat agaatcgtcc
    27121 catttcatcc aaaactggct caacgatact atccaagcta tcacgcataa agagcatgtg
    27181 attggtacga cgaagaagga agtcaactgg gctaagagtc aactcattgc gcattgcata
    27241 gtgaagagac aaggtgtctg ctaagctaag acctggcgct tgttccaagc tgtgagcaag
    27301 tgcaaagacc ttcggtgcat ttgaaccata aagatttgct aggtaatgag cttccttgct
    27361 atccaaacca cgtgaaactc caagttgcgc aaaggcttcg atttctgaat ccacatttgc
    27421 tgggttcaat tctccacctg aaacagggta agttttagaa ttgatcaatt taaagctacg
    27481 gtcaaattct gctttgagga tgtcaaccac gcgctccata gctccttcag ccatcttacg
    27541 gtagtctgtg attttaccac cagcaagagt caaaagacca ttgtcatcac ggtccaagct
    27601 agacccacga gaaactgcag atggatccaa atatttctca gatgtgctac tttcaagctt
    27661 gctgacagca gactcaacat cttcacgtgt tttttctttg gagagataag attcaacagt
    27721 cgcaatcaag ttgtcaaagc tttcatcact gatggtaccg ttatttccac cattatagtc
    27781 agaggcactg ttccctgcaa tcaatggacg aagacctgcc cagctgcttt cgatatcatc
    27841 aatggtgatg ttggattctg gaaagcggtt gttgacaatg ccaagtagat aatctacatc
    27901 ttcttgagtt acttttggat gctccaaatc acctgtgtag tctgtatcag ttgtaccaaa
    27961 gtaagtcttg ttttcacgtg ggagaacaaa gaccatacga ccgtcaccca aacctgtgtc
    28021 aaagtaaact ggctgtgaaa ccttgatttt gcttgaatcc actaccaagt gaactccctt
    28081 agttgggcgc atttgtgaga attgcgttcc cttattagac aaattacgta ctttatcact
    28141 ccaaggacct gttgtattaa taaccagacg ggccttgatt tcaaatactt ggtctgtcaa
    28201 gagatcacga gctacaacac ctgtaatctt gccactttcg tcaaggagga agccttctgc
    28261 cttcacgtgg ttggcaatga gggcaccgtc ttggttggca cgtttgatgt tttcaatcac
    28321 gagacgcgca tcgttgttac ggaagtcaag atacactcca cctcctacca agccttcctt
    28381 cttcaagttt ggctggcgtt ccaagacttg atccttgctc aaaaccttgt tcgcagctgg
    28441 tgtgttgcta acacctgcca agaggtcgta caagtccatg gctactttaa gacggaagag
    28501 gctaaaggtt gctccatctt catcgtaaac tggtaagagc attggatctg attttggaat
    28561 gtgtggagcg atttgttgaa ccactgcacg ttcagaaacc gtatctgaga ccacttctac
    28621 gtcaaattgt ttgaggtaac gaagtcctcc gtgaaccaat tttgttgaac gactagatgt
    28681 tccttctgca aagtcttgca tttcaatcaa accagtctca agaccgctag ctgccgcctg
    28741 caaggctaca ccagctcctg tgattcctcc accgataatc aagaggtcca gggtacgttc
    28801 ctgcattttt ttaattgaca attcacgtgt tttctttgaa aattccatat ttacacactt
    28861 tctaaataaa tcgctttatt ctgccagtat tagtcgtcta cttccgcaaa gacttgagtt
    28921 gctttcacag ccttcttcca gcccttgtag agttgttcct tgcgagattc gttcatagat
    28981 ggctcaaaga gttctcctgt ctcgttcaag agtttcaact cgtccaagtc tttccagtac
    29041 cctactgaca aacctgctag gaaggccgct cctagagctg ttgtttccag gtttttagca
    29101 cgtgcaatgt caatgcctaa aatatccgcc tggaactgca tgaggaagtt gttcatggct
    29161 gcaccaccat ccaccttcag tacttgaatg gcggtctgag tatccacttg catggtgtcg
    29221 atgatatcac gcacttgata agcaatagat tgcaaagtcg ccttgataaa gtcttctttg
    29281 cttgttccac gagtcaaacc aaagacggaa ccacgagcat tttggttcca gtatggagcg
    29341 cctagacctg taaaggctgg aacgacataa acttcatcgt tgttgtgaga atcacgagcg
    29401 tatttttcag attctggtga attttcaacc atgcgaagac cgtcacgaag ccactgaata
    29461 gcacttcctg cgatgaagat agaaccttcc aaggcataat aaaccttacc gttgattccg
    29521 taaccaatgg ttgtcaagag gttgttttca gacaactgca tctcttcccc agtattcatg
    29581 atgatgaaag agcctgttcc ataagtattc ttaaccatac ctggctcaaa agccaactgt
    29641 ccaaagaggg ctgcttgttg gtccccagcc atacctgaga ttggcacctc tccaccgtag
    29701 aaatggaatg gagctgtctt gccgtagatt tcggagttag aacgaacttc tggaagtata
    29761 gccttcggaa tgttaaggat ttccaaaatc tcatcatccc atttgagttc tttaatgtta
    29821 taaagcatgg tacgagctgc atttgagtag tcagtcacgt gagccgcacc gtcagtcaat
    29881 ttccaaacca accaagtatc gatagtacca aagagcaatt cccctttttc tgctcgctct
    29941 tgagcacctt ctacatgatc caaaatccaa cgaaccttgg tagcagagaa gtaagcatca
    30001 ataatcaaac cagtcttttc atggaatttt tccacataac cttggctttt tagttgctca
    30061 gccaaaggtg ctgtctggcg tgactgccaa acgatagcat tgtagatagg aagtcctgtt
    30121 ttcttatccc agacaaccgt tgtttcacgt tggttggtaa tcccgattgc ctcgatttga
    30181 tttggcttga caccactttc gatgaaagca cccgcaataa ctgactgaac agagttccaa
    30241 atttcattgg cattgtgctc aacccaacct gcctgaggga aaatctgggt aaactctttt
    30301 tgactcgagc taaccttttc cccttttttg ttgaaaatga tggcacgaga acttgtagtt
    30361 ccctggtcaa tggccatgat gtatttttct tgtgacatgt actgtcctcc tgataaagat
    30421 cttgaggtgt ttcctcttta cacttactag tatacaaaat aaagcgcttt cttgtttatc
    30481 aacttttttt aatttttaaa aaaatgtgag gagattgtgt gaatttcaca aaaaagacct
    30541 ggattaacca agccttttaa gtgaataata actggcgaat cgctaccaaa tcctccatat
    30601 ccaagtcatt ttttaaataa tagactggtg tctgttcctt cttatgaatc gggttattgg
    30661 taaccagcaa atcataatgc cgactagggt cataagcttc aatggtaata aaacgattgt
    30721 attccaaata ccgtttcaaa atggctgcca tccttgaaaa gacaagaaaa ccagatgtca
    30781 aatccagacc aatccgcata tgctggccac cgttttcagc catatattcg attaactgga
    30841 gccattccca gagaatcttc ttatctaaat ctgtcccctg ttgaaaagca ggtagagcat
    30901 gaaaaatctc ctctgctgtc tctttaaaat catgttccat caataaatat ggatgacgat
    30961 tctctaactg gtacttgtag cgatcctgta aaatatagcc cttatagaga tagacttgag
    31021 cacaaagctg actgagttca taggtgacat ggtcctctgt ataatccccc aatagttcct
    31081 ccttcttcat ttcttgaatc aattgcgtca gtaaatctgc caactgccct ccaaaaccaa
    31141 gaatatactc catagtatga agaggaagaa tgcgatgaga taggagaaaa gagaagaata
    31201 tcatcatctc accatcctca actcctagtg gaatatgttg cccagcaaaa aaggacggaa
    31261 cctttctcag caaacgaaga taaaagatat tgtcaaaata ccctcgcatt ttctcttcta
    31321 tgacttgaaa ctgacaagca ttgacccgaa gacgttgttg actgatgtga gcccagagaa
    31381 ccaagtccaa tttctgcccc gcagacaaac ttgcaccgca gatttcctct aaattggcaa
    31441 tctcctgttt tctctctggt ttctgcatgt gaccttccca ttcctgactc gaccagacct
    31501 ttcggaaaag acagaaatag aaatagcgaa tctgatgctc tggacctctc cagcgtccat
    31561 tttggatgga taaatcaaat tctgacaaaa tctgatttaa accagccaag tgacgaccaa
    31621 gcgtagcctc gctaatcacc aattcttgag ccagctgatg ggctaaaaac tgttggtggt
    31681 agagaagata aaccaaaatc tggtatttaa cagcactatc caaaaacaag ctccgaatat
    31741 ctctcccctt ggtagctgca ccgatagaca gacgcagatt ttcatcttct gagtgaatag
    31801 tcagttctaa gccactatcc aaagccttgt cattgagcag ggtgacatat ttggttaggg
    31861 ttgcctttga aaatcctgtt tcttccatta cagccttgac ggtcgtctga gactcttgta
    31921 atagaaagga aaggattgaa aattgaccac actcggcttt ctccatcaaa tctcctaaat
    31981 acatttgtta cccctttcat tttctacctt aagcatagca taaatcttac aaatgctaaa
    32041 ataatctgtt tctcttttta ttttcatgct gatttcctgg tccattatcc tgaaaatcag
    32101 taaacacacg gctccccctt tgggcatttt tatgctaaaa tagtagctat ggataaaatt
    32161 attaaaacta tatcagaaag cggagccttt cgtgcttttg tccttgatag cactgaaacc
    32221 gtccgcactg ctcaagaaaa acatcaaacc caagctagct caactgtagc gcttggtcga
    32281 actcttatcg ctagccagat tctcgcagcc aatgaaaaag gaaataccaa acttacagtt
    32341 aaggtgttgg gatctagctc tctaggtgct attatcaccg tcgctgatac caaggggaac
    32401 gtcaaaggct atgttcaaaa tcctggtgtt gacattaaaa agactgcgac tggtgaagtc
    32461 ctagtcggac cttttgttgg aaatggtcaa ttcctcgtta tcacagacta cggtactgga
    32521 aatccttaca actctataac tcccctcatc tctggagaaa tcggtgaaga ccttgccttt
    32581 taccttactg aaagccaaca aacgccttca gcggtcggcc tcaatgtcct tttggacgag
    32641 gaagacaagg tcaaggttgc aggtggtttc ctagttcaag tcttgccagg agccaagaaa
    32701 gaagagattg ctcgctttga aaaacgcatc caagaaatgc cagctatctc tactcttctc
    32761 gaaagcgacg accatatcga agccctcctc aaggctatct acggagacga agcctacaag
    32821 cgtctttctg aagaagaaat ccgtttccaa tgtgactgta gccatgaacg ctttatgaac
    32881 gctcttgcca gccttccaag ctcagactta caggaaatga aagaggaaga ccacggggta
    32941 gaaatcactt gtcaattctg ccaaactact tacaactttg atgaaaagga cctggaggaa
    33001 ctcattcgtg acaaatctta atacaccttt tatgattggc aatgttgaga ttcccaatcg
    33061 taccgtttta gcgcctatgg ctggcgtgac caactcagcc tttcgtacca tcgcaaaaga
    33121 gctcggagct ggactcgttg taatggaaat ggtctctgac aagggaatcc aatacaacaa
    33181 cgaaaaaacc ctgcacatgc ttcatatcga tgagggcgaa aaccctgtct ctatccaact
    33241 ttttggtagc gatgaagaca gcctagcacg cgcagcagaa ttcatccaag aaaacaccaa
    33301 gaccgatatc gtcgatatca acatgggctg ccctgtcaac aaaatcgtga agaacgaagc
    33361 tggtgctatg tggctcaagg atcctgacaa gatctactct atcatcaaca aggtccagtc
    33421 tgtccttgat atcccactta ctgtcaaaat gcgtaccggc tgggcggacc catctctggc
    33481 agtagaaaat gctctcgctg ctgaggctgc aggtgtttct gccctcgcca tgcatggccg
    33541 tacccgtgaa caaatgtata ctggccacgc agaccttgag accctttaca aggttgccca
    33601 agctctaacc aagattccat tcatcgccaa cggtgatatc cgtactgtcc aagaagccaa
    33661 gcaacgcatc gaagaagttg gtgctgacgc agtcatgatt ggccgagctg ccatgggaaa
    33721 tccttacctc ttcaaccaaa tcaaccatta ctttgaaaca ggagaaatcc tacctgattt
    33781 gacctttgaa gacaagatga agatcgccta cgaacacttg aaacgattga ttaacctcaa
    33841 aggagaaaac gtcgcagttc gtgaattccg cggcctcgct cctcactatc tccgtggaac
    33901 atctggcgct gccaaactcc gtggagccat ttcgcaagct agcaccctgg cagagattga
    33961 agccctcttg caattggaga aggcttaata gtttaaaacc cgtaactctc ttaaagagcc
    34021 tcttgaatgc cgccattctt tcctcatcaa aaaaactccg tagtctctag cgctataaat
    34081 ccggctagag attacagagt tattttttta tctaaacttc ctacaagggt atattatcta
    34141 ctgaaaggat atggtatttg tagggaatac tttaaagagt tcttacagga agtcagtatt
    34201 aactagttat attaggttta gtttacccat tcaccattgg cattgactct atagctatct
    34261 acagttgtgt tgactgcaag ggcacctgag ccattgacat agtaccattt atctgatact
    34321 ttgaaccatt ggcttgcttt catagcacct gatgcttcaa gatagtacca ggtatctcca
    34381 tctttcaccc aatctgtcgc cattgaacca ttagcgttta ggtagtacca tgagccattg
    34441 ttttggagcc atcctgtcgc cattgaacca tcagtattgt agaagtacca cataccgttt
    34501 tcttgtttcc agcctgtttt tggagtagat ggttgtgctg gtttttcagt ttttggcggt
    34561 tgctgttgag tcaagcgatt atattcttct tctgatctac gagcatagtc ttcttctgct
    34621 tgttgatcag ctggtttttc tgcttttggt tgttcagctg gattctctgg ttttggagct
    34681 ggagctggtt tttctggttg aggagccggc gctggttgtg gttgttcagc tggtttttct
    34741 ttaactttat cttcttctgc tgcttttcgt ttagcttctt cttctgcttt tttacgatct
    34801 gtcttgattt tttctaacct tgtagcctca gcttgtttac tctcaacttc cgcttttgct
    34861 tgcttaactt tttcctcgtt tcgaggttcc ttagcttcct cttttactag ttcaagctcc
    34921 gcttttttaa cttccacatc ggactcagca atttcaagtt caagcgtttt gtaagtattg
    34981 gttgggtagt tacggcgatc ttcttctttt tgatccttgg cttttttctc agcttctgca
    35041 accttcttct cagcttctgc tacctttttt tctggtttca gggatgggct tggaagagtt
    35101 tcttcaccta cgctagaatc tgaagacttc gcatcatttt cttttttatc aggtgttgct
    35161 tgctctccaa gagctcctcg ttttacccgc ctctttggtt caccttgctc tgaagtcgct
    35221 acattagctt cctgcaactt agcatctgct tttcgtttag cttcttcttc tgcttcttta
    35281 cgctctgtct tgattttttc taaccttgta gcctcagctt gtttactctc aactttcgct
    35341 tttgcttggt taattgtgtc ctcgtttcga gttttagctt cctctttcaa tagttcaagc
    35401 tccgcttttt taacttccac atcggactca gcaatttcaa gttcaagcgt tttgtaagta
    35461 ttggttgggt agttacggcg atcttcttct ttttgagcct tggctttttt ctcagcttct
    35521 gcaaccttct tctcagcttc tgctaccttt tctcctagtt tcaatgtatc ttttttaaac
    35581 ttctcaaaag ctgcggttaa ctcttctttt gtttttgacg tcaactcttc ttttttcgac
    35641 ttctctttta aaacatttaa ttcataaaaa tacttcgttt gaattatgct caactttttg
    35701 ttgaagttga aattttgggt atgttttctt ctatctaatt tgatcccact caacatttct
    35761 tctagatgcg acttgacttc atcagcatac ttcttcgata tatcttgccc actagatgta
    35821 accgtggggg tattcctacc accaacctct tctgcatgaa ctactcctcc taagaacaag
    35881 ctagcaacta ctacactagc tactccaata ctaaatttgc gaattgaata atgtactttt
    35941 ctttcgcttt ttgatgcaaa cataattatt tccttctata ttttttcttt aaccttacta
    36001 tatcacttca atatgaactg tatataaatc gaattttgta tttatctctt tatgcttcca
    36061 tttgggatta taacttcatg taatagcttt tcctcttcag ctattcgtcg atacaaaata
    36121 actgcataaa ctggaaaaac taaaacaaaa gtcacataag catgactcaa caaggtcaag
    36181 ccaatcaact caggaagaat atttaaaaag taattaggat gttttactgt cctaaacaag
    36241 atatgatcta catatttgtg attattgaca agcataagct tcactgtcca aatatctccc
    36301 aacaagtgaa tcaccaacat taacatcagc atagaaaaaa taagcaagac taaaccaacc
    36361 atgcccatgc catcaaaaat tgtcttgtgc accattgcct caaccaagca agagagataa
    36421 aaaacaatat gagccatagt taatcgtttt gtattctcaa ctccaaattc tttcccgcca
    36481 ttgctaagga ttcgtttctc attctctatc gaacgcttta aaaaaatcaa tcgaataagg
    36541 aatgttacta aaataatcgc taataccata ttagtatcct ttcttttatc tacacaaaga
    36601 ataacacact tatgttaacc ctatatgaac tttaataaaa aactaatctg tctacaagct
    36661 aatcttaaat tccataccgt gttcataagg gagaaaacta taatccattt gatagctttc
    36721 taaaatatta ctcacaatgt aaagacccaa accggaactt tcatccttat ctgtcacgat
    36781 ttgagaatga tagaatatat caaataaatg ttctagttct tggtcactta gaggcgcaca
    36841 tgtatttttg atagagagat agccatcttg ctcggatatg attacgcgcc catttttatc
    36901 tgaatattta attgcattac taatcaggtt tgtcaaaacc ttatccaatg ccctaagact
    36961 catgacgacc ctggtagcat ctgtcaattg attttcaatt gtaacaccta ttgaatgagc
    37021 taattcttga taacgtgata aaatatctac taaaactggc ttaacagtca aggtctcacg
    37081 acactctgtc cactcttgga atttagaaga ctccagtact tcttccaata agtggctcat
    37141 ctggtcaatc ttatttatac tctttgcaat atattttgga tgatctttgt aatctccaat
    37201 attatgctgc atattctcta ggataattct aagactggct aaaggggttt tcaactcatg
    37261 tgatgctcca cggacaaagg aaaccttttg attttgcaat tttacaattt gctcattacg
    37321 actttccaac tcatgaataa ccttcaacaa gtgctcatac ataccattaa tctgttttcc
    37381 aacttcacca acttcatctt tcctactttc atcaaaacga atattgtcat ccaaatcttg
    37441 cattttacta gtcacttctg aaatgtaaaa aagaggattg agcaagcgtt tagtataaaa
    37501 ataagaaaaa acaaaggaaa aagcaattgt aaccaaaaat gtatatggga gatacaaaag
    37561 caaaatattc tttgcttctt tgtagacatc caccccgtga acaaattgca ctgttacctg
    37621 tttaccatct gctgtactaa cagacatatc taacatcacg atataatttg atagctttcc
    37681 atctttaata tccaagtcat gcacaagagg gagtcgctta tctacaatat ctcttttaag
    37741 gtgagcagat atggtcaagg actttgaata agactggagt aagcttggaa tttcatcaga
    37801 tgtcttagtc tttaaatact ccgtaaacac tcttgcgctc tcattaaact tttccccctc
    37861 cagttgaata taataaaaag gaaaggtcaa ataaatagct atatgaatga ctagaccaac
    37921 accacctaga attgcaaaac ttcttaaaaa agacttggtt aataatttag gattttttat
    37981 cataagctaa tcttataccc aacatttttc acagtcacga tacaatctaa agctaatttt
    38041 ttgcgaagag tacgaatata gacatcaacg accctatcac aaggtaaatc agctacatct
    38101 tttgaaattt cttccaatat ctgctctcta cttaaaactt gatttttatg ctgaatcaaa
    38161 cactttagta ccagtaattc ctttggtttg agatcaattt cttcattttt ataatgtgct
    38221 ttgtaagagg taaaatccac tgttacatcc tgatatcgcc aaagatcctc tatgacgtag
    38281 taacgtctga taagcgcctt aatacgcttg acaagcagta ccaaagaaaa aggttttgtc
    38341 acatgatcat cagcatagag atcaaatacc tgtgactgag tagtttcatc tcccagagca
    38401 ctcaagacga tgacaggaat atcgtgcttt tgattgattt cttttaacac ttcaaaacca
    38461 ctcttcctag gcatcattaa atccagtacc atgagatgga tagggagatc ttgaaatttt
    38521 tccaagacct cttgtccatc cttagccata atgacatgat aaccctcttc tgtcagaaat
    38581 gctgcaattc cttctctaat catttcctcg tcatctgcaa ctaaaatgtt catctctctc
    38641 cctttctact atcagaactc catacgtcta tttcctgtat gctatttgaa aatttcctac
    38701 cagtctatca tctatgataa tggaatggat ttcatttatc cgctactttt tgtcaacaac
    38761 aacgtcaata caagaaaatc attcatcttt cagcttatac tcttcgaaaa tctcttcaaa
    38821 ccacgtcagc tctatctaca acctcaaaac agtgttttga gcaacctgcg gctagctgcc
    38881 tagtttgttc tttgattttc attgaatatt agtttgattt tttaaatacc agctagaacg
    38941 gatttgacat gcaaaaaaga aacaggtctc ttttgaaaga ataaaaattt cagataagac
    39001 tgctcttttt ctactacaca tgctctaaga caaatacgac ggagggtaat aaacatatga
    39061 attttgtgtt gaatggtgaa aaccacgaat cgtttagaag ctataggttt tagtttcata
    39121 ctcttcgaaa atctcttcaa accacgtcag ctctatctgc aacctcaaaa cagtgttttg
    39181 agcaacctgc ggctagctgc ctagtttgct ctttgatttt cattgagttt cactccactg
    39241 tgacttatgg tcagatgctg atacttttat tcttttatgc aatatcaaat tttaactggc
    39301 ctgctttgac accaatctta agtgtgctgc ctgccactaa atctccctta agaagaagtt
    39361 ctgccaactt gtcctccact tctgtttgca gggttctgcg aagtgggcga gctcccatct
    39421 ctgggtcata tccttgattt gctaacaatt tcagagctga agcttgtaat ttcaagtcaa
    39481 tgcctttttc agccaaactt gccactaaag gcttgaccat aatcttcacc acttcctgca
    39541 tatggtcact agataggcta tggaagacca ccttctcatc aatacggttg atgaattccg
    39601 gtctataagc ttttttcagt tcttcaaaca tgcgtttttc catattttcc tggtcaaaac
    39661 gaatatcctt agccccaaaa ccaacagtct tatcatcacg aagggcagtc gcacctagat
    39721 tcgatgtcat aatgataatg gtatttgaaa aatcgacctt gcgtcccttg ctatctgtca
    39781 agacaccgtc atccagaacc tgcaagagaa cattaaagat atctgggtgg gccttctcta
    39841 cctcatcaaa gaggagaacg gaatagggtt tattgcgaac cttctctgtc aactccccac
    39901 cttcttcgta acccacatag cctggaggag ctccatttag acggctggct gcgaatttct
    39961 ccatatactc actcatatca aagcggataa gggctgattc gtcatcaaag agaacttctg
    40021 ccaaagcctt ggccaactcg gtcttaccga cacctgtggg gcctaggaac ataaaggaac
    40081 cgatcggacg cttgtgactg cgaattcctg actgattacg gcgaatggca cgactaatac
    40141 ttgaaacagc ttgatcttga ccgataaccc gtttatggag ttctgcttca agatttaaat
    40201 acttcttagc atccgtttga gtcagttttt gaactgggat tcctgacaag cgactcaagg
    40261 tggtcaaaat atcagactct gtcaccaagt ctttgtagac aggtacttcc tcttcttttg
    40321 cgattagctg ggctgcctgt ttccacttgc catccatcag ggccttgtca gctggactca
    40381 aatctgaatc gtctgctttt acatactttg ccttattttg cactgttgct gctgcttcgt
    40441 ccaagaggtc gatagcagag tctggcaagt gacgactggt taaataacga tgagccatct
    40501 taaccgctgt ttcaaccgct tcatctgtga tttgtacacg gtgatgtttc tcataagtca
    40561 ccttcaaacc ttgtaaaata gtcatactat ctgccacact tggttcttca atcgtcactt
    40621 tagcgaaacg acgagaaagt gccgcatctt tttcgatatg tttttgatat tcttcctgag
    40681 tggtggcacc aaccgttctc aaagttccac gcgccaaggc tggtttcaag atattggccg
    40741 catccagagt cgaatcaatc ccgctaccag aacccatgat ggtgtggagt tcatcgataa
    40801 agaggatgac ttggccatct tcttcaatat ccttgatgat attattcatg cgttcttcaa
    40861 agtcaccacg gaagcgtgtc cctgcaacga cattcatcaa atcaagttct aacacgcgca
    40921 tcttagccat ttccgcaggc acgtcaccac tggcaatacg ctgggcaaga ccaagcgcca
    40981 gagctgtttt cccgacacca gcatccccaa ccaagacagg gttgttctta gtcttccggc
    41041 tcaagatttg aatcatacgt gagatttcct tgtcccgacc gatgactggt tctaacttgc
    41101 cagaacgcgc ttgctctgtc aaatcatgcg tatagtcctc gagaccacca ctaggagtct
    41161 gcggcatgcc catcatattg gccatagaat tttgcttgtc agctactgta cgatggcgtt
    41221 ggcgtaaagc cttgagatct tcacgagtcc agcctgcccg ttcttctaaa tttcgacgaa
    41281 gagcagcaat cttgacctga tctttcttgt cttcataaga aaaaccagcc ctctccaaga
    41341 tacgagtcgc caaggcattg ccatcatgca aaatcgcata gaggacgtgc tctgtcccta
    41401 gtaccttagc atggaccact gacgctacat actctgcttc gtcaaaaaga acctgcaaac
    41461 gacgggagaa cggcaattcc gtaaaggttt catcctggct atagtccgtt tcagtcagtt
    41521 ccaaagccac ctcttctaaa cggtccatct catacggata atcatttaaa gttgcccctg
    41581 ctacactata actgtgatta gacatggcaa tcaacaagtg ccacgactct agataacgag
    41641 ctccaaaatg tccagcaacc atgtaggcac tttcgataca ttcattcaat gcttttgaat
    41701 agttcatctt acttcccttt tctatctacc tcttgtatga cctgacgtag catgtttgct
    41761 cgaacaactg gagcttcttc tcctaaaacg cgatccaaag ctactgattc tagcaaattc
    41821 atctcctgct tggtcatcaa ttcctgctca accaaaagct ggagaatatc ctcataaatt
    41881 tcttgactga ctcgctcacc aatcgagtaa agcagctccc ggagcatttc atgatgacta
    41941 gaaaactcaa tccgtcctat acgaatgtag cctccgccac cacgcttact ttcaaccaag
    42001 tagcctctac tttccgtaaa gcgtgtcttg atcacgtagt taatctgact aggaacaacc
    42061 tgaaaggtat ctgccaactg actccgttgc aactccacga taccagattg atctaaaatc
    42121 gccttgatgt aggcctcaat atgatccgat gtatttttaa atctcataac aaacccacct
    42181 ctctctttaa accttgacct tgtttgacta ttataccgaa attttctcat ttttaaaaga
    42241 aaaagggcgc tggtaaagga taatcttcac caactcccta tttttctact tatctaagcc
    42301 taattctgcc aagatttgac gtttgtaggc aatcttttgg acttccttgt cctcatcttc
    42361 agaccaatct agtttaattt ctgaaacaat ctgcccaggg cgatttttca agatatagat
    42421 gcggtcgctg agattgaggg cctcctcaat actatgcgtg atgatcaggg ttgttagctg
    42481 caactgtttg tgaatctcaa gataccaagc gtggagttcc atctttgtca tctcatccaa
    42541 ggcgctaaag gcctcatcta agagaaagag cttgtgccca aaaaggtagg tccggagtaa
    42601 ggctacacgc tggcgcatcc caccgctaag ttcatgagga tacttgtctc ttacagctgt
    42661 cagctggaag gtcgcaagaa ttttatccgc tcgggaaata gcttctgcct tatccacctt
    42721 ttgaatcaag aggggcagaa tgatatttcc aagcaccgtc ttgtgctcca agagcagatc
    42781 cttttgcaac atataactca cgcgcccctg gggattttct tcaccatcaa ggacaattct
    42841 ccctgactga acttctaaaa tcccagcgat tagattaaag agggtggtct ttccaacacc
    42901 acttgggcct aggatggaaa ccacttcgcc tgaagtcacc tgtaggttga tatcctctaa
    42961 aatcctctcc tgaccatagg cataactgac gtgctctagt ctaatttctg tcattatttc
    43021 acaaattcgt tggtgaagcc tttgtctgtc aagtcttctt taaggatacc attttcttta
    43081 tcccatttgt agaaagcatt ccagcgagct gcgtcaaatt gaccccattt ttccttgtcg
    43141 cttgcgtatt cttttgacaa gtatttttga gattcgatga caaagtcacg tttttccttg
    43201 agttcaggtg cattcttgat gagaatatct gcagcttctt ctggatgttc catggcatat
    43261 tggtagcctt ttttgatggc ttggatgact ttgcgagctt cttctttgtt atctttcaga
    43321 tagtcgttgt ttgcgatgat aactggtgaa tagtagtcaa actccttgac atagtctttc
    43381 aagtacatga agttagcatc tacaccttga gatttagcaa ggataccatc ccaaccgtag
    43441 taaatccaag cagtatcaaa gacgccattg gcaatcggtg tgattgagtt tgagtcgtta
    43501 tttggtactt tttcaacctt ctcaaagtct ccaccttgag attctaccaa ggttttcaac
    43561 atagcaagtt cagttgggtc attccatgtc ccatatttct taccaaccaa gtcttttgga
    43621 ctgcttacat tatcagattt acgagagatg attcctgatg tattgtgttc aacaatagct
    43681 gcaacggcag tgattcctgc tcctttttcc aatttcttag ccatgtagtc ttggaaatac
    43741 actgcaaatg gtgcctttcc gttgataacc aagtcagaag aactttcttc tggtggcaat
    43801 ttcaaatcaa catccactcc agcttctttg aaataacctt tttccttggc aacataaagc
    43861 cctgtgtggt tggtatttgg tgtccagtct aggataaagt caaccttctt aagttctgcc
    43921 tctttgttgt ctttagaagc agttccttga ccacaggcca caagcacaac agctacaaga
    43981 gctgttacaa gcgttaaaaa cactttccat gttttcttca ttttgatttc ctcttttctt
    44041 tttcagaaac attctaattc tacgaacgtt tccatttaat aacatatttt tcactaatat
    44101 cgaccaactt catacccaaa aggctgataa tcgataccag aataataata gcgaacatgg
    44161 tatcatactg aaacaatttc ttggactgaa tcatataaac accaagcccc tcaaagcctc
    44221 ccaaccactc agataccact gttgtgataa aagcgtagga gacactgacc ctcagaccag
    44281 cataaaagta gggcaggctg actggaattt taaaatgcca caggatttgc caaggcttgg
    44341 cccgcatcag actaaacaag gtcagcatat ccttatcgca atgcctaaaa ccatccaaaa
    44401 tgctgacgat gatgggaaag gttgtcgtta agataatcaa gacaatcttg ggcaaaatcc
    44461 cgtaacctag ccacaagacc aggataggag ctatggcaat ggtcggaatg gtctgaataa
    44521 ccaccatcat agggtaaatc aggtcattga gccaagtcaa actatccatg agtacagcca
    44581 taagacaggc aatcaaaact cccaaaatca gccccagtaa agccactctc aaggtcgccc
    44641 agctatggtg ccagagaaat tctctgtcac gaacaaaggg ctggagaatt tcaagaggtg
    44701 tcggcaggat aaacttgggg agcagtttaa gaaaacctgc taactgccag attgacaaga
    44761 ctccgagaaa gcccaataga ctaatgtgtc gtctcagtat acttctcaag tttctcatca
    44821 atacttaaaa tctctcctac atttattttg acattggcaa agacattgtc tgcctcctgc
    44881 cctgccactt ccagcgcttc ttttagaatg cgcataagct catcaaactc cccttccaag
    44941 accgtttcaa atggtgtcac taccatcgtc acttcttgag tttgcagata agcaatgacc
    45001 tgatcaataa cagctatccg atcaatcccc tgtgctaggg gtaaaacttg caaggcaatg
    45061 cttgctttca taagaacctc ctcttctcgt tttccttttt aaaaaaagaa aaacctttac
    45121 catacatgga aaaggtgcaa aattagacta agtatcgttc cctccgctgg cattacccag
    45181 atcaggtgcg gtcgaagttt aacacttcct ctcagactgt acacagactc ccatatatta
    45241 tatgtatata tgatagcgca aaataaaaaa aatgtcaagg aaactgctta cagaaaagag
    45301 caggaaattt ctcctactcc aattttctat actcgttctc catcactgtt tactctgtag
    45361 ccatccaccg tcgtattaac agctaaggcg cctgaagagt aagaataata ccacttacca
    45421 gagacctggt accagcctgt tttcattgcc cctgaactac ccagataata ccacgagcca
    45481 ttctccttta accatcctgt ctgcatttca ccagataatt tcaaataata ccagctagaa
    45541 ccatctttca gccatcctgt tgatttagaa ccatttgatt tgaaatgata ccaactacca
    45601 tttattttct tccaaccgac agttgaacta ttattggatc catagctaga gatatttcct
    45661 gattgattct gataattttg tgaagaagtc actccctcta gctgtatata gcgacgactt
    45721 ccgttataag ccgtataact caaccacttg tagccgtctt tttcgagaat ttgatcataa
    45781 tgaaccttct ctccaggata atagtaatca atcacagttg cactaactag gggttcagtt
    45841 ttgatagcag acttggtctt aaaataatga gttccgcctg ttgaagttga ggattgacta
    45901 ttcccaacac tgccaccatc caaatcttta aaatgaataa atcctgtcat cgtgtttgct
    45961 tttataattc gtttattata ggattctgta taaccatagt tatattcctc aatctcaatc
    46021 tgatctccca ttacatttga cacccaggca acatgaccat aagttcctgc agtagaccaa
    46081 gtaatggaac caatcgtcgg tgtattatct acacgataac cttcccgacg agcacgatgt
    46141 ccccattcat tcgcatttcc ataagctgcc ggaatttcaa aaccattgac attactcaaa
    46201 cgaaaggcta caaaagaagt acactgacga gaatacatgc gccactgatc aatctcctgg
    46261 ctcccatttt tataataagc aggataatca tccccacgcg caatcgatcc atttcctctg
    46321 gaataggcat agacactatc accagctgct agcatcaatc ctactactaa aaaaggaaca
    46381 agctttttaa ctgacatctt caagtaataa cttgttcctc ttgctataaa cggtaaaatt
    46441 ttcattcttc ctccttgaaa aataatataa agcgatgatt gccttcacct aaattattcg
    46501 gagaaaaaag aaaaagtgag aaaatttctc actttagtca agataatgaa tcaaattctt
    46561 ttctgtaaga atatctgagt aagtaaagga ttcaacgtaa acattaactt gtttatctcc
    46621 ttccacatcc ccaaactcca caataaatag agatggataa acttcaatta gcttacccaa
    46681 tctatttttt tggcgcttac gaccattctc caaagtcatt tctacgactt gtccctcatg
    46741 tgccttgatt tcttctttga tttttttcat cttggctaca tctgtaaatg catctgacat
    46801 cttatgcctc cctctttgag atacttgaaa atttattgta ttctttttgg aaaatcaatt
    46861 ccactgttcc acgagctcca ctacggtttt tctcgataat aacttccacc ttattatttg
    46921 ggataccctc ctcttcttca ccaccacgtt catagtagtc atcgcgatag agaaaagcta
    46981 cgatatcagc gtcctgctca atagaccccg attcacgaat atcagacaag accggtctct
    47041 tgtcctgacg ttgttctaca ccacgagaaa gctgactcag agcgattact ggaaccttca
    47101 attccttggc tagtattttc aactgacgag aaatttcaga aacttcttgt tgacgatttt
    47161 ctcgaccagt tcccgtgata agttgcaaat agtctatcaa aatcaaacca agatttccag
    47221 tttcttgagc caatttacga gaacgagaac gaatctctgt aatccgaata cctggcgtat
    47281 catcgatata gatactggcg ttagctagat taccctgagc aatagtatat ttttgccact
    47341 cctcatctgt caattgccct gtacggatag aatgtgactc cactaagcct tctgcagcta
    47401 acatacgatc taccaagctt tccgcaccca tttcgagtga aaaaatagca accgttttgt
    47461 ccaacttagt cccaatgttc tgagcgatat tcaaggcaaa tgctgtctta ccaactgctg
    47521 gacgagctgc taagataatc aactcctcct catgaagtcc tgttgtcata tgatccaaat
    47581 cacgataacc tgtcgcaata cctgtaatat cggtcgtttg ttgcgagcga gcttccagat
    47641 ttccaaagtt gagattcaac acatctcgaa tgttcttaaa cccgcttcga tttgcatttt
    47701 cactgacatc aatcaaccct ttttctgcct gagcaataat ttcatcagct ggttgtgacg
    47761 cttcgtaagc ttggttgaca gactctgtca acttggcaat taaacgacgt agcattgctt
    47821 tttctgcaac aatcttagca taatactccg cattagcaga agttggcaca gaattaacaa
    47881 tctcaaccaa gtaagacaag ccaccaatat tctgtaaatc accttgatta tcaaggatag
    47941 tacgaaccgt tgttgcatct atggcatcac cacgatcgga taaatcgacc atggcttgga
    48001 aaatcaaacg atgggcatac ttaaaaaagt cccgagactc aatgtattct cgcacaaaaa
    48061 caagtttact ctcatcaata aagatagccc ctaaaacgga ttgctcagct aagatatctt
    48121 gaggttgtac tcgtaactct tctacttctg ccatcagact tcccttcctt ttacaatctt
    48181 gtcaagaaag tgtaaactta tccttctttc acacgaagat tgattacact tgtgatatct
    48241 tgatagattt tcactggcac atcaatcaaa ccaaccgctc gaatcggagc ttgtacttga
    48301 atatgacgtt tatcaatctt aattccaaat tgcttttgca attcttctgc aatcttctta
    48361 ttggtaatag aaccaaaggt acgaccatct ggaccaactt tttcaacaaa ttctacaaca
    48421 gtttcttctg cttcaagttg tgctttaatt gcttttcctt ctgcaatcat ctcagcgtga
    48481 gctttttctt ccgatttttg tttacctcga agttcaccta cagcttgagc agtcgcttct
    48541 ttggctagat tctttttgat aagaaagttt tgcgcatacc ctgttggtac ttccttaatt
    48601 tcgccttttt taccttttcc tttaacatct gctaaaaaga ttactttcat tcttctttct
    48661 ccttttcctt tatttcattt aatacaattt ctgtcagttt ttcacctgct tctgacaagg
    48721 ttacatcttt aatttgagct gctgccaaat taaagtggcc tccaccgcct aactcttcca
    48781 taatccgttg tacattcagt ttactacgac ttcgagctga gatagagata aatccttgtg
    48841 tattcttcgc aagaacaaaa ctcgcttcaa tacctgacat ggctaacatg gcatctgctg
    48901 ccttactaat aacaactgta tcatagcatt tcgtgtcctt agcctctgct attagtacat
    48961 ctgaacctaa tttacgcccc tgtaaaataa gttcattgac ctcacgatat tcttcaaaat
    49021 ctgtcgcagc gatttcctgg atagcaatac tatcacttcc gcgcgttctg agatagctag
    49081 caacatcaaa tgtccgacta gttactcgcg aggtgaaatt tttagtatcc aacatcatac
    49141 cagccatcaa aacacttgct tgcatacgac tcaaacgatt tttcttagaa ttctggaact
    49201 gaatcaattc cgttaccaac tcactggcac tacttgcacc actttcgata taagtaataa
    49261 ccgcattatc tggaaaatcc tgatcccttc tatggtggtc aataacaatg gtttgggtaa
    49321 ataaatcata aaattctttt gataatgtta aggctgtctt tgaatggtct acaagaatca
    49381 acaaagaacg attggtcacc atccccattg catccttaac agacaacaac ttcgtaactc
    49441 cttctttttc tatgaatgaa acagctcgtt caatatctgg agacatttgt tcttcatcat
    49501 aaagagcata gctattttca atcacattgc tggcgaacaa ctgcatacct acagcagagc
    49561 ccaaagcatc catgtctaaa tttttgtgac cgactacaaa aacctgatct acactccgaa
    49621 tcttatctga aatagctgtc atcatagcgc gcgtacgagt ccgtgtacgc ttgattgaag
    49681 cagcagaccc accaccaaaa taaactggat ttttcgtttc gtcgttttcc ttaacaacca
    49741 cctggtcgcc accacgtact tcagccaagt tcaaattgag caaagcaact ttccctatct
    49801 catcatgatt tccatcgcca taagaaaatc ccatacttaa ggtcaagggc aactgtctct
    49861 gtttcgactc ttctctgaaa gcatcaataa cagaaaattt atcattcatc aagccctcaa
    49921 gcaccgtgta gtcagtaaat agataaaatc gatccatact tacccgacga gaaaacatca
    49981 tgtgtttttc tgaaaactct gatataaaat tagctacaaa actattgatt tgactaatat
    50041 ctgactcaga agtttcatcc tccaaatcat cataattatc cacagagaca atcccaatca
    50101 ctggtctact tgttaccaat tcatctgtta tggcttgttc cctggataca tctacaaaat
    50161 acaaaacacc ggaagaagca tccatatgaa cagcataacg cttctcacca agcttggcat
    50221 aagtagacgg atttcctact gaagccttga taatcgtttg aacagcttct aaatcaaaat
    50281 caccatcttc cttggtcaaa atcaattcag catagggatt aaaccactca acctctccag
    50341 aagataaatt caatttcata acacctacag gcatctgttc caatagagct gtcaaacttt
    50401 cttccgcttg gtggtttaca tactgtatct gttctacatc actccttgta taatgcactc
    50461 tcagtttctt aaataaaaaa acatagcctc ctacaaaaag aaacaaaatt aaaaccgtca
    50521 acagattatt attaacaaaa ataatgaaag tggataagac tccaaacgca atcaatccta
    50581 ctagaatagg aaaaattgga cttacataaa attttttcat tcaaaacctc ttggcaccca
    50641 ttataccata atacccctca aaaagcgact ttttaaaagt gtaatcagta attctatcaa
    50701 ttataagaaa aaggtagttt acaattcagt aaacctacct ttacacatat tgaaattaag
    50761 attctttaac ctctaacaaa ccaatttcgc catcctcacg acgataaatc acattggttg
    50821 tctgatcttc aacatccaca tagataaaga aatcatgccc caataaatcc atttgtagaa
    50881 ttgcttcttc caaatccatt ggttttaaat caatttgttt tgaacgaaca actttagact
    50941 ggacaatatt tgaatcttcc accaaagcat ctgtaaataa ttgaccagtt gctaccttat
    51001 ttttattttt acgctcgatt tttgttttat ttttacgaat ctgacgttca attttatcag
    51061 ttacaaggtc aattgaacca tacatatctt gagatacatc ttctgcgcgg agagtaatag
    51121 atccaagcgg aatcgttact tccactttag ccgttttttc acgataaact tttaagttaa
    51181 ttcgggcatc caactcttgt tctggttgga agtacttttc gatcttttcg agtttagaaa
    51241 ctacataatc acgaattgct tctgttactt ctaggttttc accacggata ctatatttaa
    51301 tcatatgagt accttctttc taaacatttt tgtttttatg attttattat aacgctttca
    51361 ttctattttt gcaaattttt tcctcatctt acaagggaaa atgtttttac atccttagca
    51421 ccggcttctt ccaacagttt cttaacacga tttatagttg ctcctgtagt atagatatca
    51481 tctataagta ggattttttt aggaatagtg actccacttt taataaagaa aggaagttct
    51541 gtccccaagc gctctgaacg atttttagaa gaactggctc tctcttctct tttctctaat
    51601 aaatctagat actcaaagcc tgctgcctct accaagccct caacctgatt aaatcctctg
    51661 ttagcatatc tatcaggact taggggaatt acaacaaatt gatactcttt gtactttttc
    51721 aactcctcac ttaaaaatga agcgaaaact tttcttaaca ggaagtctcc atcaaactta
    51781 taccgactga aaaaatcctt catagcttga ttgtaagtaa aaatcgctct atgactgact
    51841 tcaactccct ctttacacca aagttgacaa tcttgacact ttgttgacaa ctctgttttc
    51901 atacaatttg gacagttctc ttccccaatt ctttcaaaag tagaatcaca gtctgaacaa
    51961 agacaagagt catcattcct cagaagtaag agactactaa aagttaaaac agtcttcata
    52021 gtctgcccac ataacaagca cttcatagac cagcctcctt attcatcatc tgaatttcct
    52081 taatcgcctt cttgattgaa gcatttaacc catcatggaa gaaaagcaaa tctcctgtcg
    52141 gtctatccat gcttcgtcca actcgtccac caatctgaat caaactagac ttggtaaaca
    52201 aacgatgatt ggcctctact acgaaaacat ccacacaagg gaaggtaact ccgcgctcca
    52261 agattgtcgt actgataagt attgtcagtt ctccatctcg aaaagcttgt acttgctcta
    52321 atcgatcctc tgttacagaa gatacaaagc caattttctc atttggaaat tgctcctgta
    52381 agatttctgc taactgctcc cctttcttaa tttctgaagc aaaaatgagt aacggataag
    52441 ctgtctttct ctgcttctca atataggact ttaactttgg tgacaaacga ttcttgtcta
    52501 agtagcgatt aaaatccgat aaccaaattg gttttggaat aatcaacgga tttccatgaa
    52561 accgtctcgg taaattcagt ctttttagtt ctcctaaacg gaccttttta tctaactcat
    52621 tggtcgaagt cgctgttaaa aagattctca atccattctc ctttacacta ttcttgacag
    52681 cgtggtaaag catgggatta tcaacataag gaaaagcatc tacttcatcc actatcagca
    52741 aatcaaaagc ttgataaaac ttcaataact gatgggttgt tgcaacaact agtggtgttc
    52801 gaaaataagg ttccgattct ccatgtagca aagctatccc gcaagaaaaa tcctgttgca
    52861 ggcgcttgta cagctccaaa caaacatcta tgcgaggact agccaaacac actgcaccac
    52921 ccgcattgat cactttagcc actacttgat aaatcatttc tgtctttcca gctcctgtta
    52981 ccgcatgaac taaggttggc ttttgcttgt ctactacttg aagcaatccc tctgacacct
    53041 tctcttgaaa aggagttaat tggccgcgcc atttgagaac atcttgcttt ggaaaatcct
    53101 cctgcggaaa atagtataaa gtttgatcac ttctgactcg cttcatcagc aagcactctc
    53161 gacaatagta agcaccgatg ggcaaatacc attcttctag aatagtacta ttacagcgtt
    53221 gacagaaaag tttccccttc tcctttctca ttgctggaag tttctccgcc aactgacgtt
    53281 cttcttctgt taattcattc tcagtaaata aacgaccgag ataatctaaa tttactttca
    53341 tacttcttta ttcgtaaaaa ctagcacttt agatgatttt ttagtacaat taaatcatgg
    53401 aatttaggac aattaaagag gacggtcaag tccaagaaga aatcaaaaaa tctcgcttta
    53461 tctgccatgc caagcgtgtt tatagcgaag aagaggctcg tgacttcatt actgccatca
    53521 aaaaagaaca ctacaaagcg acacataact gctctgcctt cattattgga gaacgtagtg
    53581 aaattaaacg tacaagtgat gatggtgagc ctagtggtac tgctggtgtt cccatgcttg
    53641 gggtactaga aaatcacaat ctcaccaatg tctgtgtggt cgtgacacgc tactttggtg
    53701 gtattaaact aggcgctgga ggactaattc gtgcttacgc cggcagtgtc gccttagctg
    53761 tcaaagaaat tggtattatt gaaataaaag aacaggctgg cattgctatt caaatgtctt
    53821 atgctcagta ccaagagtac agtaacttcc ttaaagaaca tggtctcatg gagctggata
    53881 caaactttac agatcaagtc gatacgatga tttatgttga taaagaagaa aaagaaacta
    53941 ttaaagctgc acttgtggag ttttttaatg gaaaagtcac tttaactgac caaggtttac
    54001 gagaggttga agttcctgta aacttagtgt aaacaatgaa taatacagcg tttcgttgac
    54061 attctcacaa ctactttagc gagcaaaata aaaagaggcg taccaaaata tactagaaaa
    54121 tgaagcaatt caaacgaaac ctgatatcgt tttccttcac acctatttac tagaattagc
    54181 tgaacgcaat cacttgaaaa ttaatgactt tgatctatga tatatagaaa tggtatggat
    54241 agcgttatac taaagatatc ttatacaaag aggtattcat atgtctattt ataacaacat
    54301 tactgaatta atcggtcaaa caccgattgt taaacttaac aacatcgtgc cagaaggtgc
    54361 tgcagacgtc tatataaagc ttgaagcatt taatcctggt tcatctgtaa aagaccgtat
    54421 tgcccttagc atgattgaaa aagctgaaca agatggtatt ctgaaacctg gttctactat
    54481 tgttgaagca acaagtggaa acaccggtat tgaactttca tgggtaggtg ctgctaaagg
    54541 gtataaagtc gtcatcgtta tgcctgaaac tatgagtgta gaacgacgta aaattatcca
    54601 agcttatggt gctgaactcg tcctaactcc tggtagcgag ggaatgaaag gtgctattgc
    54661 taaggctcaa gaaatcgctg ctgaacgtga tggtttcctt cctcttcaat ttgacaatcc
    54721 agctaatcca gaagtacacg aaagaacaac aggagctgag atactagctg ctttcggtaa
    54781 agatggatta gatgcctttg ttgctggagt aggtactggt ggaacgattt ctggtgtttc
    54841 tcatgcactc aaatcagaaa attctaacat tcaagttttt gcagtagaag cagatgaatc
    54901 tgctattcta tctggtgaaa aacctggtcc tcacaaaatt caaggtatct cagctggatt
    54961 tattcctgat acacttgata ctaaagccta tgatggtatc gttcgtgtaa catcagatga
    55021 cgctcttgca ctcggacgtg aaattggtgg aaaagaaggc ttccttgtag ggatttcctc
    55081 agctgcagct atctacggag ccatcgaggt tgccaaaaaa ttaggtacag gtaaaaaagt
    55141 ccttgcccta gcaccagata acggtgaacg ttatctctct acagcacttt atgaattgta
    55201 accgtccaat aacgaagtct attgaaaaat ctccagacta gagaactcac ggatagttcc
    55261 taatctggag atttcttatt tgcacttttc ttgtacaact ttagtccatg gtaaataggc
    55321 ctctaaaacc tctttgttta cgagagtttc cacgtttgga agacattcta gaagatagga
    55381 tagatatttc tcactattta taatggattg aaataagata tgaacaaatc gattagaaca
    55441 tgatggtaaa gcgtaatccc ttgtttctca gctttccc
//
LOCUS       DAVKGC010000003        51516 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKGC010000003 DAVKGC010000000
VERSION     DAVKGC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.003"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            100..1554
                     /locus_tag="VW049_000111"
     CDS             100..1554
                     /locus_tag="VW049_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9431315.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            1535..2500
                     /locus_tag="VW049_000112"
     CDS             1535..2500
                     /locus_tag="VW049_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9431316.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(2535..3809)
                     /locus_tag="VW049_000113"
     CDS             complement(2535..3809)
                     /locus_tag="VW049_000113"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9431317.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNDQEYTVPYVTEEPSVVAAASYAIKIIKRTGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(3809..4981)
                     /locus_tag="VW049_000114"
     CDS             complement(3809..4981)
                     /locus_tag="VW049_000114"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9431318.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(5112..5360)
                     /locus_tag="VW049_000115"
     CDS             complement(5112..5360)
                     /locus_tag="VW049_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431319.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(5428..6054)
                     /locus_tag="VW049_000116"
                     /pseudo
     CDS             complement(5428..6054)
                     /locus_tag="VW049_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6116..6565)
                     /locus_tag="VW049_000117"
     CDS             complement(6116..6565)
                     /locus_tag="VW049_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9431320.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(6567..7325)
                     /locus_tag="VW049_000118"
     CDS             complement(6567..7325)
                     /locus_tag="VW049_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9431321.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(7440..9419)
                     /gene="pknB"
                     /locus_tag="VW049_000119"
     CDS             complement(7440..9419)
                     /gene="pknB"
                     /locus_tag="VW049_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9431322.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(9416..10156)
                     /locus_tag="VW049_000120"
     CDS             complement(9416..10156)
                     /locus_tag="VW049_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9431323.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(10171..11484)
                     /gene="rsmB"
                     /locus_tag="VW049_000121"
     CDS             complement(10171..11484)
                     /gene="rsmB"
                     /locus_tag="VW049_000121"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9431324.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGIEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(11477..12412)
                     /locus_tag="VW049_000122"
     CDS             complement(11477..12412)
                     /locus_tag="VW049_000122"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9431325.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDNRYEILAVATQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSICKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(12425..14821)
                     /locus_tag="VW049_000123"
     CDS             complement(12425..14821)
                     /locus_tag="VW049_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9431326.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVFGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVRRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(14887..15201)
                     /locus_tag="VW049_000124"
     CDS             complement(14887..15201)
                     /locus_tag="VW049_000124"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9431327.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(15226..15852)
                     /gene="gmk"
                     /locus_tag="VW049_000125"
     CDS             complement(15226..15852)
                     /gene="gmk"
                     /locus_tag="VW049_000125"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9431328.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(15982..17595)
                     /locus_tag="VW049_000126"
     CDS             complement(15982..17595)
                     /locus_tag="VW049_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9431329.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(17786..18040)
                     /locus_tag="VW049_000127"
     CDS             complement(17786..18040)
                     /locus_tag="VW049_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU9431330.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(18044..18298)
                     /locus_tag="VW049_000128"
     CDS             complement(18044..18298)
                     /locus_tag="VW049_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU9431331.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(18536..19633)
                     /locus_tag="VW049_000129"
     CDS             complement(18536..19633)
                     /locus_tag="VW049_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9431332.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGTEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARESDLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAQQNFGRVPIRIETN"
     gene            complement(19643..20389)
                     /locus_tag="VW049_000130"
     CDS             complement(19643..20389)
                     /locus_tag="VW049_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9431333.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNVLNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(20492..20845)
                     /gene="rsfS"
                     /locus_tag="VW049_000131"
     CDS             complement(20492..20845)
                     /gene="rsfS"
                     /locus_tag="VW049_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9431334.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(20858..21358)
                     /locus_tag="VW049_000132"
     CDS             complement(20858..21358)
                     /locus_tag="VW049_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9431335.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEGWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(21359..21952)
                     /gene="yqeK"
                     /locus_tag="VW049_000133"
     CDS             complement(21359..21952)
                     /gene="yqeK"
                     /locus_tag="VW049_000133"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9431336.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRSFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(21952..22581)
                     /locus_tag="VW049_000134"
     CDS             complement(21952..22581)
                     /locus_tag="VW049_000134"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9431337.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRAFLAQGRKPNFLLPQLVLDYIEKEGLY"
     gene            complement(22633..22944)
                     /gene="yhbY"
                     /locus_tag="VW049_000135"
     CDS             complement(22633..22944)
                     /gene="yhbY"
                     /locus_tag="VW049_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9431338.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(23159..24265)
                     /gene="yqeH"
                     /locus_tag="VW049_000136"
     CDS             complement(23159..24265)
                     /gene="yqeH"
                     /locus_tag="VW049_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9431339.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(24268..24858)
                     /locus_tag="VW049_000137"
     CDS             complement(24268..24858)
                     /locus_tag="VW049_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9431340.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(24810..25718)
                     /locus_tag="VW049_000138"
     CDS             complement(24810..25718)
                     /locus_tag="VW049_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9431341.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(25775..26335)
                     /locus_tag="VW049_000139"
     CDS             complement(25775..26335)
                     /locus_tag="VW049_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9431342.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            26474..27683
                     /gene="sstT"
                     /locus_tag="VW049_000140"
                     /pseudo
     CDS             26474..27683
                     /gene="sstT"
                     /locus_tag="VW049_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            27751..28704
                     /locus_tag="VW049_000141"
     CDS             27751..28704
                     /locus_tag="VW049_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9431343.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(28802..28879)
                     /locus_tag="VW049_000142"
                     /pseudo
     CDS             complement(28802..28879)
                     /locus_tag="VW049_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(28940..29164)
                     /locus_tag="VW049_000143"
     CDS             complement(28940..29164)
                     /locus_tag="VW049_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEU9431344.1"
                     /translation="MIDILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(29169..29348)
                     /gene="asp4"
                     /locus_tag="VW049_000144"
     CDS             complement(29169..29348)
                     /gene="asp4"
                     /locus_tag="VW049_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEU9431345.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(29350..30687)
                     /gene="gtfB"
                     /locus_tag="VW049_000145"
     CDS             complement(29350..30687)
                     /gene="gtfB"
                     /locus_tag="VW049_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEU9431346.1"
                     /translation="MIELYDRYSHESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGTCFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHYPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKTTYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLCYPNVALY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYVAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(30680..32191)
                     /gene="gtfA"
                     /locus_tag="VW049_000146"
     CDS             complement(30680..32191)
                     /gene="gtfA"
                     /locus_tag="VW049_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEU9431347.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLRDIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(32203..34575)
                     /gene="secA2"
                     /locus_tag="VW049_000147"
     CDS             complement(32203..34575)
                     /gene="secA2"
                     /locus_tag="VW049_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEU9431348.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(34568..35008)
                     /gene="asp3"
                     /locus_tag="VW049_000148"
     CDS             complement(34568..35008)
                     /gene="asp3"
                     /locus_tag="VW049_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEU9431349.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(35005..36540)
                     /gene="asp2"
                     /locus_tag="VW049_000149"
     CDS             complement(35005..36540)
                     /gene="asp2"
                     /locus_tag="VW049_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEU9431350.1"
                     /translation="MSKELNILQVGLTNWENHYDIPENMNWYYFYPNSSKALREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFAGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(36524..38104)
                     /gene="asp1"
                     /locus_tag="VW049_000150"
     CDS             complement(36524..38104)
                     /gene="asp1"
                     /locus_tag="VW049_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEU9431351.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHYIMDHRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFREHLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKL
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(38114..39331)
                     /gene="secY2"
                     /locus_tag="VW049_000151"
     CDS             complement(38114..39331)
                     /gene="secY2"
                     /locus_tag="VW049_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEU9431352.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRIILPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSYSKKLGLSSTAIEIQDRR
                     KMYLTLLIAVIQSLAVSLSLPVQSSYSVILVALMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYKPLI"
     gene            complement(39388..40065)
                     /locus_tag="VW049_000152"
     CDS             complement(39388..40065)
                     /locus_tag="VW049_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9431353.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSALRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(40050..40294)
                     /locus_tag="VW049_000153"
                     /pseudo
     CDS             complement(40050..40294)
                     /locus_tag="VW049_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(40294..41490)
                     /locus_tag="VW049_000154"
     CDS             complement(40294..41490)
                     /locus_tag="VW049_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU9431354.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNTGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGIDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYMNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(41499..42719)
                     /locus_tag="VW049_000155"
     CDS             complement(41499..42719)
                     /locus_tag="VW049_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEU9431355.1"
                     /translation="MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(42744..43628)
                     /locus_tag="VW049_000156"
     CDS             complement(42744..43628)
                     /locus_tag="VW049_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9431356.1"
                     /translation="MKDLISVIVPVYNVEPFISSCLDSLSKQIYQNFEVLLVNDGSTD
                     NSGAICREYADRDSRFHYFEKENAGVADARNFGIERSKGDYITFVDSDDWVTEEYLSI
                     LIETLKEQHSEIVVSTYSTYNESDGLFYTHVFDSDYFVKNYNSKLLMKELPLLEGYDM
                     SFLTSWGILFKRELFQEVQFPFGRLCEDIGTNYKLFMQVEKVIYINKVLYWYRVGKEG
                     LSNSYSPKMMRDDCDFRLERIAVLALKGYDVSKYLDQMKFYLKYRHDIAIQRELKENV
                     ETRRLEMLDYLLNGNKYN"
     gene            complement(43625..46078)
                     /locus_tag="VW049_000157"
     CDS             complement(43625..46078)
                     /locus_tag="VW049_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEU9431357.1"
                     /translation="MNKTIVLAGDRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSIYLEEKSLAAVKDTDGITFNTGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWSQILQHHMGEFELISPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTNMGDKLIKLAVYNNVRLHPQIVPPVLD
                     KLERSVDLYLDINYSHVVDTILEDMKILEKPILSFDTTEHGNTGQLVFKKDEVSVMVQ
                     AIKDYRRDGRFLSCYEGSDFHCLTFTNSQELQKIDYLVKNFPMVTFHIAAWTVMGPML
                     QELAKKYKNVKLYPEVKSDQFEELKSQMDVYLDINLHNSTSEVVKEISSLNKPMLAFY
                     ASQNGNHGQHLYSSEHPERLLQAFHALIKGETLGEPEKPIKVIGIDQTLDYVIKNKSS
                     LVRFGDGEVNLMWGLPIPYQNHDLELANQLKHIVGLESDEKLVVCLPDAFDDRFVFTW
                     WATPFWKEHMNVYMDFYKELCKGSWYGSTFISRPYIDYEDKSKAKGQFEKLKSIWENR
                     DILIVEGITSRSGVGNDLFDKVKSVKRIICPSHNAYSVVDNIQEEIMKHAEGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQILVDLSKREV"
     gene            complement(46088..47098)
                     /locus_tag="VW049_000158"
     CDS             complement(46088..47098)
                     /locus_tag="VW049_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9431358.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIS
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSNARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNDGESNQYYTLNISHKVSTYLTAGIPVIVPSNLSTAKFIV
                     DQGLGFMADSLEEVHAIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     ID"
     gene            complement(47127..47717)
                     /locus_tag="VW049_000159"
     CDS             complement(47127..47717)
                     /locus_tag="VW049_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEU9431359.1"
                     /translation="MEHDALDRLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDD
                     SLEVIEGKAIMDREGVEEVRNGNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVL
                     LRASRIVYLNRCVYWYRVGLSDTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHI
                     LIYKNRLQRVVAKLEEQNMQFTEIYRRMMEKLSLLP"
     gene            complement(47732..48001)
                     /locus_tag="VW049_000160"
     CDS             complement(47732..48001)
                     /locus_tag="VW049_000160"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9431360.1"
                     /translation="MENELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGRTLLL"
     gene            complement(48041..48919)
                     /locus_tag="VW049_000161"
     CDS             complement(48041..48919)
                     /locus_tag="VW049_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEU9431361.1"
                     /translation="MKKNHLVGDALILKVSDQIEELDYLLESLPNICFHIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLFVKRRD"
     gene            complement(48916..51000)
                     /locus_tag="VW049_000162"
     CDS             complement(48916..51000)
                     /locus_tag="VW049_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9431362.1"
                     /translation="MDDKITVIVPVYNVENYLRKCLDSIITQTYKNIEIVVVNDGSTD
                     ASGEICKEFSEMDHRILYIEQENAGLSAARNTGLNNMSRNYVTFVDSDDWIEQDYVET
                     LYKKIVEYQADIAVGNYYSFNESEGMFYFHILGDSYYEKVYDNVSIFENLYETQEMKS
                     FALISAWGKLYKARLFEQLRFDIGKLGEDGYLNQKVYLLSEKVIYLNKSLYAYRIRKG
                     SLSRVWTEKWMHALVDAMSERITLLANMGYPLEKHLAVYRQMLEVSLANGQASGLSDT
                     ATYKEFEMKQRLLNQLSRQEESEKKAIVLAANYGYVDQVLTTIKSICYHNRSIRFYLI
                     HSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKSDISYTVFLRYFIADFVQEDK
                     ALYLDCDLVVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNAFWKK
                     ENMTQKLIDVTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQLPEG
                     QDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIYPIKE
                     PFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNGIHYL
                     VDVDNELVETSQVLLDINHGEKTEEILDQFANLGKPILSFENTKTYEVGQEAYAVDQV
                     QAMIEKLREISK"
     gene            complement(51061..>51516)
                     /locus_tag="VW049_000163"
     CDS             complement(51061..>51516)
                     /locus_tag="VW049_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9431363.1"
                     /translation="SASASASTSASASASTSASASASTSASASASTSASASASTSASA
                     SASTSASASASTSASASASTSASASASTSASASASTSASASASTSVSNSANHSNSQVG
                     NTSGSTGKSQKELPNTGTESSIGSVLLGVLAAVTGIGLVAKRRKRDEEE"
BASE COUNT        15085 a        11602 c         9168 g        15661 t
ORIGIN      
        1 taatcgtttg acagtttttt ctctttttta atataaaagg cttgcatttt ttatcgaaaa
       61 cagttactat taaaagtgat aagatttttg gaggaatgaa tggaatggac aactgagcgt
      121 cgttacagac tttatcaaga ttggacgcaa gaagaaattc aacatataaa ggaaaatatg
      181 gcacaatctc catggcatac tcattaccat gttgagccaa aaacaggact tctcaacgac
      241 ccaaatggct tttcttactt tgatggcaag tggatcctct tttaccagaa ttttcctttt
      301 ggtgcagccc acggtttgaa atcttgggca cagctagaaa gtgatgattt ggttcacttt
      361 aaagaaactg gaatcaaagt tttaccagat actccattag atagccacgg tgcctactct
      421 ggttctgcca tgcaatttgg cgataactta ttcctatttt atacaggaaa tgttcgcgat
      481 gaaaactgga tccgtcaccc ataccagatc ggtgctttga tggacaagga gggtaagatt
      541 acaaagattg acaagatctt gattgaccag ccagcagact ctactgacca cttccgcgat
      601 ccacaaattt ttaactttaa aggtcaatac tatgctgtcg tcggtggaca ggacttggag
      661 aaaaaaggct tcgtccgtct ctacaaggct gtcaataacg actacacaaa ctggcaagca
      721 gttggcgacc ttgactttgc taacgaccgt actgcctaca tgatggaatg tcctaatctg
      781 gtctttgtag aggaacatcc tgtccttctc tactgtccac aaggattgga taagaaagtt
      841 ctagactacg ataatatctt tccaaatatg tataagattg gggcttcctt tgaccctaaa
      901 aatgccaaaa tggtagatgt gtctcaactt caaaacatgg attacggttt cgaagcctat
      961 gcaactcaag ccttcaacgc tcctgatggg cgtgctctag cagttagctg gcttggtttg
     1021 ccagatgttt cttacccatc tgaccgtttt gaccaccaag gaaccttctc tttggtcaag
     1081 gaactcacta tcaaagacga caagctctac cagtatccag tcgctgctat taaggacctt
     1141 cgtgcttctg aagaagcctt ctcaaaccgt tcccaaacca agaacactta cgaacttgaa
     1201 ctcaacttgg aagctaatag ccagagcgag attgtcttac ttgctgataa agaaggtaag
     1261 ggactttcaa tcaactttga ccttgtaaac ggtcaagtga cagtggatcg tagccaggct
     1321 ggagagcagt atgcccaaga atttgggaca actcgttctt gccctatcga gaatcaggct
     1381 actactgcta caatcttcat cgataactct gtctttgaaa ttttcatcaa taaaggagaa
     1441 aaagtatttt ctggtcgtgt cttcccacat gcggaccaaa atggtatcct gatcaaatct
     1501 ggaaacccaa ctggaactta ctatgaatta gattatggtc gcaaaactaa ctgatgtcgc
     1561 caaacttgca ggcgtcagtc ctactaccgt ttctcgggtt atcaataaaa aagggtatct
     1621 atctgagaaa accatccaaa aagtcaatga agccatgaga gaattgggct ataaacccaa
     1681 caacctggct cgtagtctgc aaggaaaatc agctaagtta atcggcttga ttttccccaa
     1741 tatttccaat gttttctatg cagaattgat tgataaattg gaacaccaac tcttcaaaaa
     1801 tggttacaag accatcatct gcaacagtga acatgattct gagaaggaac gcgaatacat
     1861 cgaaatgttg gaagccaatc aggtggacgg catcatttct ggtagtcaca acctaggaat
     1921 cgaagactac aatcgtgtga cagcgccgat tatttccttt gaccgaaacc tatcgccaga
     1981 catccctgtc gtctcctctg acaactatgc tggtggggtt cttgctgccc aaaccttggt
     2041 caagacaggt gcccagtcta tcatcatgat tacagggaat gacaattcta attcgccaac
     2101 cggactgcgc cacgctggtt ttgcatccgt actcccaaaa gctcctatta tcaatgtttc
     2161 cagtgacttt tctcccgtca gaaaagaaat ggaaatcaag aatatcttga cccgggaaaa
     2221 accagatgcc atttttgctt cggatgattt gacagctatt ctggtcatta aaatcgctca
     2281 agaattgggc atttctgtcc caaaagagct caaggtcatc ggctatgatg ggacctactt
     2341 tatcgaaaat tactaccctc aattggctac tatcaagcaa cctttggaag agattgcttg
     2401 tctcactatt gatcttctct tgcaaaagat tgaaggcaag gaagtcgcca caactggtta
     2461 cttcttacca gttacgctat taccaggaaa aagtatttaa acacaagaaa actcagaccg
     2521 attcgtctga gtttttatga tcttaaattt tcgagatagc gctgggctgt ctctaggtta
     2581 aaggttttat ctgagatgag gcgctctact aggggagcaa cttcagattc actagcccca
     2641 gctaggagag ctagagattt ggcctgtagt ttcatgtggc cttgctggat gcccgtactt
     2701 accaaggctt tgagggctgc aaaattttga gcaagaccga tggacacgat aatctgggct
     2761 aattctctgg cagaaggatt tcctagtaga tcatgactga gagctacacg tgggttgagg
     2821 ccgatagagc cacccttagt cgctacaggc atgggcaggg tcatctcacc gaccaattct
     2881 tctctttcaa ggtccagcgt ccagcagcta agaccttgat agcgtccatc tcgactggca
     2941 aaggcatggg ccccagcttc gatggcacgc cagtcattac cagtggcaat caaaatcgca
     3001 tcaataccat taaaaattcc tttattatga gtagcagctc ggtaaggatc agcctgcgca
     3061 aactgactag ccaacgcaat tttctccgca atctctcgtc cttgatcctt ttggcggctc
     3121 aagtagcgaa aggcgatgcg acagcttgca gtcaccagag aatcagtcgc gtagttggac
     3181 aggattccca tgagactctg tccctgactg agttcttcta agactggttt caaggcttcc
     3241 agcatggtgt tgagcatatt ggcacccatg gcttcctggg tatcgacatg aatataaaca
     3301 acgagaaagt ctggttcgcc ttttatctgc tcgacatgca gatcacgcgc cccacctcca
     3361 cgtttaacga tagaaggata ggcttgattg gcaagctcca agagctccgc tttcttgctg
     3421 gcaatcttct cttgcgctag tttaggatta gcaacttgat aaagggctac ctgcccaatc
     3481 atctgtcgct gatggacttg tgcagtaaaa ccacctgtac gcttgatgat tttgatggca
     3541 tagctggccg ccgcaaccac agagggttct tctgtcacat agggaacggt gtattcctga
     3601 tcgttgacaa gtacctccgg aaccagcgaa taaggcagag aaaaagttcc caccacattc
     3661 tcactcagct ggtctgccac agtcacactc atctgttcat ccttctccag actagcttgt
     3721 ctctcaggac taaggagcgc ctgagctttt aacagctcga ggcgctcttg gtatgatttt
     3781 ttagaaaatc cattccaact tatcttcatt atttttcaac cttgctataa cggcgttggt
     3841 ggtcaagaat ttcaaccaag gcaaaatctt gattttcata gccagcaaac tgggcagagt
     3901 ttgtttcatc caagtttact tcctcaaaaa agaccttttc atagtctgca acggataggg
     3961 cagttcgttg gttgagcttg ttcaaacggt ctttatccaa ataagcttca tatccttcaa
     4021 ccaattcacc actgaagaac tcagccacag ctccacttcc gtaactataa agggcgattt
     4081 tatccccagc tttcaagcta tctgtatttt ccaagagaga caaaagtcca aggaaaagtg
     4141 aacctgtgta gatgttcccc accttttgac tgtagagaat agactggtca aaatgctttt
     4201 gtaagaggtc ttttttctct tgaggcaggc tcttatccat gatttttttc aagcctttta
     4261 gcgctaattt aggataaggc aagtggaaac aaacagcagc aaaatcatcc aaagtaagct
     4321 ggtagcgttt ttgatattca agccaagtcg ttttcaaact atccaagtat tgttgggtag
     4381 aataaacacc atttacataa ggagttgtcg agtaatttgg tcgccagaaa tccatgatgt
     4441 cacgggtctg agctacattg tcattattaa aggccatcat gcgtggattt tgtgtaatca
     4501 acatagctac acttccagca ccttgagttg gttctcccgg agtttcaata ccatatttgg
     4561 caatatcact ggcaatgacc aataccttgg actccggaga attttccaca tgcaatttgg
     4621 cataatggag ggcagctgtc gccccataac aggcttcttt aatctcgaaa ctacgagcaa
     4681 agggctggat gcccagcaag ccatgcacaa agacggccgc agccttactc tggtcaattc
     4741 ctgactcggt cgccacaatg accatgtcaa cttcttgtct ttcttgctca gttaaaatag
     4801 agtcactagc actggccgcc aaggtcacga tatcctcagt taggggcgca atactcaatt
     4861 ccttgagtaa gagtccttta cttaattttt cagggtcaat tcccctcgct tctgctaagt
     4921 cttgtaattt caagacatat tgactggtcg caaaaccaat cttatcaata ccgattgtca
     4981 tatttacctc tgttttatca ttcatgtaaa aaatcgttct atactatttt atcacaaatg
     5041 gcagtaaaag agagaaaaaa gacttgattc accaaatcaa gcctcttatt ggtcatcatt
     5101 ttaaagaatg attagttgct agagagttca ccgatataag tagctttata agctccattc
     5161 acagttatca gctcctggag gatcaaattt cctgagtaag tccttcccat ctcatctaca
     5221 aatttttgat aaaactgact ggtcggaatt tctctgacat ccttatcaaa tgtcttgtca
     5281 agtgttttac taaccttctc agcaatcaat tgatgctctt gccatccact ttgaaactct
     5341 gagcccgaac tagaaaccat gactgggata aacaacaagg tcagtagact tacagacaat
     5401 aaggaaaata gtagacttcc tgcaaaacta gaatcctagt tcataattga taatgccagc
     5461 aatcaaattc attcgtaatc cgaagcgttt acgatgattt cgataggttg ttgaaaacat
     5521 tttaaacgtt tttactttgg caaagatgtt ctcaaccttg cttctatcct tggatagcgc
     5581 atggttataa gctttatctt cagctattag cggcttgagt ttgctggatt tacgtggagt
     5641 ttgtgcttga ggatatatct tcatgggtcc ttgataacca ctgtcagcca agatttttcc
     5701 agcttgtccg atatttctgc gactcatttt gaacaacttc atatcatgac aatagttcac
     5761 agcgatatcc aaagaaacaa ttctcccttg acttgtgaca atcgcttgag gttccataga
     5821 atgacatttc tattttacca gaataattgg ctagttgatt tttttaggac gatttatttt
     5881 tacctctgtt gcatccacaa tcaccgtatt ctcagcacta agatgagttt ttgaaatcgt
     5941 aacaccactt tgaacaagag ttacttcaac ccattggctc cgacggatta agttgctttc
     6001 gtggatacca aaatcagccg caatttcttc ataagtgcgg tattctcgca catatagaaa
     6061 gcgttgtcaa tttatttatc tcatttttca gaaaattctt ttatttctgt aaagtctacg
     6121 atactcgatg tgtttttata taatgataca gtctgagaat cactgttccg ctagccattc
     6181 caatagagat taccaaagcc aacatgacaa ccaaggtcgc acttgccagt gctttattat
     6241 agtcccctgt cacaaaaaag gcagttgttc ggtaggagag ataacctgga accagcggtg
     6301 ccaaaatggc caagataaag accacagcag gtgtcttata aagaatactt aaaatctggc
     6361 tgacacaaga accaataatg gctgcaatga aggtagctac aatgacattg gtcggttcct
     6421 tgagcaagag atagattagc cagacagtca tgcccaaaat ccctccaggt aagagcatag
     6481 accgttgcac attgagtacg attaaaaaag tgataatggc aagaaaactt gctactgctt
     6541 gtaataaaaa ggttgttagt gtcatattag ttcatcaata ccaaggcgac agaagttcct
     6601 gcccctaaag cgagggtaat gagcagggat tcaaacatct tactcatacc agagtttatg
     6661 tggttggtca taatatcacg gaccgcattg gtcaaggcaa tacctggtac aaacggcatg
     6721 accgcaccag ctataatcaa atctgccgtt gaaggaaaac ctgtgtagcg agcccaaaac
     6781 tgggcaatta tcccaaagac aaaggctcca gcaaaggctg tcacaaaggg aattcggata
     6841 aatttttcca catagaggga aaaggcaaaa ccaaataagg tcgccactcc tgccccaagt
     6901 gcgtcgtaga tatttccgct aaacataact gaaaagaaag gagcactaaa ggtcgcagcc
     6961 agagttacct gcaacttagt atagggaagg ggttgagctt gcaaggccgt caattgctta
     7021 aaggctgttt ctaagtcaat ctgcccccca actagctgac gagaaatctg gttcacatcg
     7081 cagacttttt cgatgttata agaagaggag gtcacgcgct tcatgcgcga aatattggta
     7141 ttttcaatag agaaaaagat agcggcaggc atggcaagga cattgcaatc cacaatcccc
     7201 tgcgaatgcg cgattcgaat catggtatct tctacacgat ggatttctga gccactttta
     7261 aggagaatag tccccgctag cataatcaca tcaatgacgg catttaattc ttttgattct
     7321 tccatgcttt cctcctttta tcaactccct ctattctatc acaaatctgg actcaaaaaa
     7381 aatctttgcc atgaaatcat gacaaagatt gattactcat tttgattatc catctgcttt
     7441 taaggagtag ctgaagttgt tttaggtttg tagattgaaa tcttgactct agtcttattg
     7501 aggtctacct tttcacctgc tctaggactt tgttcaacaa ccatgccttc tgcactacct
     7561 gcaggcgctg tcgtcacttc tacaacttct atattagctt ccttaatccc aacaatttga
     7621 atcaaattgt tcttagtaaa ctccaagctg gaaccaatgt aactcggcat ggcaacactt
     7681 gtaacttttt tagctactgt caagacaatt tgagtaggtt tactcacatc ataagtcgtt
     7741 ccggcacctg gactttgttt cataatcgtt cctggttcgc tttcgctgga ctcttcttcc
     7801 tctatcttaa tcaaattctc aggaaccttc ttctgcttga gttctgagat tacttctgta
     7861 gagttccgtc caatatagtt ccctaattga atcgtcgtag cttttttagc tactgtcaaa
     7921 acaatttgag ttgccttgct caagtcatag gtcgtacctt ctggtagact ttgcttcagg
     7981 accgttccag cctcactctc attcgactct tcttcctcaa ttttaatcaa attatctgga
     8041 acttttttct cttttaattc cgcaatgaca tcagaggatt tccgaccgac ataattacta
     8101 atttggaaag attgcttgcc tgatgagaca accaaattga ttttcgttcc ttcttttcga
     8161 ccagttccag cgccaggatc tgtacggata atccgccctt cttccacctt ttcactagcc
     8221 tctgtcttct cctcaccaat ctcaaaattg gcttttttga gcgttgcctt ggcctctgca
     8281 actgtctgac ctgccacatc tggaatggca atggttgcag gagttctgga tagtatccaa
     8341 ataagagaag ctgccaccaa tacaaggctg gccaacaaaa tcaggtaacg catcttaaat
     8401 ctatgttttt tcggtgcttg tggttggtaa gtttcctctg tcacagcctg gcttgggttt
     8461 ttgattgatt tgtgttctgt ctgcgcttga accttaggaa tagatgtcaa ggtactctga
     8521 gaaaccttcg gcaaggtctt ggtatctgcc ttgctcgttt catcaaagat taacttactt
     8581 tcatttctac gattgtagga caagctacta gacaagtcca catacatctc tgaaaccgag
     8641 cggtagcgat tggtcaactt tttagcagtt gccttgataa taacattttc taaagcctga
     8701 ggtacagatg gattttctgc aataacggac ggcaggggtt tctggaaatg ctggagggca
     8761 atggtcaccg cgctatcccc gtcataaggg atatggcctg tcaacatctc atagaaaata
     8821 atccccatgg catagatatc actctgcaca gtcgccttcg aaccacgcgc ctgctctggt
     8881 gacaagtaat gaactgagcc caacatcgag ttagtctggg tcagacttgt ctctgcaaag
     8941 gctacagcaa tcccaaagtc tgtgaccttg gcagtcccat ctggtgtcaa aaggatattt
     9001 tgaggtttca agtccctgtg aacaattcct cgagtatggg ccaagcgcat agccaagaga
     9061 atttgtccca tgatacggac tgcttcttca ttagaaagag gataatgttc cttgatatag
     9121 cgtttgaggt ctagtccagc aacatactcc attgcaagat actgttgacc gtcttcctca
     9181 ccaatatctg ttatccgaac gatatgagga tggtctagat ctgccatagc tctcgcttca
     9241 cgctgaaaac gagctacagc tatcgggtcc gtctggtagt tggtcctcag aaccttcact
     9301 gccacttctt ccccatctaa gattaagtct ttggctaggt agacatccgc catacctcct
     9361 cgaccaatct gtttgacaat ccgatagcgt ccggcaaaaa tcttgccgat ttggatcatt
     9421 ctgcatcctc ctcgttcata gaaacaaggg caaccgtaat gttgtctaaa cctcctgcat
     9481 tgttagcaaa acgaacaagt gtctccgttt tatctgctaa aggaatatca ctggttacaa
     9541 tatcacgaat ctcactgcct gaaatcatgt tggtcaagcc gtcactattg agcaagagat
     9601 agtcacctga ctcaaggata actgtcccaa aatcaggctg aatttcatct ttttgcccaa
     9661 tagactgggt gataatattt ttttgcggat gagcttctgc ctcttctggt gtcaattgac
     9721 cagccttgag caattcatta accaaggaat gatcgctcgt caactgatgg tattcttctc
     9781 cacgaatcaa gccgatacgc gaatcaccaa tatgagcata gatagcctga ttatcaataa
     9841 tagcaaggac ttccaaagta gttcccatgc ctctgtaagc ttcatcctga ccaagctggt
     9901 gaatcttttg attttcaatt tctaggtaat gggcgaacca ttcacgcact tcattgactg
     9961 tatcgatctg ggtatcaacc caagctacac ccaggtctgt gaccgccatt tcactagcga
    10021 tattccctgc gcgatgacct cccatcccat cagctaaaat aatcatggta cgtccagctc
    10081 tattgacata gtggttgaca tagtcttggt tatttgttcg tttctgacca acatctgtta
    10141 ataatgaaat ttccatgtgt cagttccttc ctaatccgat atcttgcgaa attgactgat
    10201 gaagaatcca tcacttccat acaattcagg tgtaatgagg atacagccgt ctttcatgat
    10261 atccttacat tcatgttcta gttttacctg ctcgaactcg ggatgactct ctaaaaaggc
    10321 cttaacgact tgaaaattct cctctgagac gatagtgcag gtgctataag ttattatacc
    10381 acctttgcct agtatttgac aaacactacc taatatttct aactgaattt cctgcaagga
    10441 cgcgaaatct gccgtttctt tattgtattt gatatctggt tttcggcgca aaagaccgat
    10501 tcctgaacaa ggagcatcca ccaaaatctt atcaaaggaa tcctggtcaa aaaactcatg
    10561 cacctttctg gcatctaatt tttgagtttg aacccgatct tcaattccca gacgttgggc
    10621 attttcttga attaaatcca acttgtggtc gtacaagtcc agagcagtaa cctgacctgt
    10681 cgtaagataa gaggctatat gggctgtttt cccacctgga gccgcacagg catcaagcac
    10741 tcgctcatca ccttgtaaat caagcgtcgg agcaaccagc tgactggact cgtcttggat
    10801 ggtaatggct ccatccgcaa acaaattatg ccctgcaaaa tgcccttgct ccttaaccag
    10861 accagtggtt gctaaaaggg aatcattcgc ctccaacaag gcttggattt cctcttttcg
    10921 acttaggtct gttacacgaa tactggcttt gtttcgcact aacaggcttt caaagatggc
    10981 ttttgctctc tcctctccgt attcttcctt gagtttggca actagccaaa ctgggagaga
    11041 ataggcaatg gagtcacgct tgttttttcg cttgatgcta gcaatatctg gccagccttc
    11101 acgcaagata cggcgaagga cagcgttgac caatttttca ctgccttttt tacggagttt
    11161 ggctaattcc actgcttcat tgaccacagc atgatctgga atcttgtcca aatagcggag
    11221 ttggtaggca ctcatgagaa gaaggacata gagccagctg tctaactggt ctctgtcttc
    11281 gataaagtgg gataggtacc attccagagt cagtttacgg gctaccgttc catagactag
    11341 ctcggtcact aagcccttgt ctgctgcaga gagctgactc cccttgaggt gtttatttaa
    11401 ggcaatattt gaatatgctt gattcacaaa aacatcctct agcactgcta gagctaaact
    11461 tctagccgtt tctactttag tcaccaaatc gttctcctac agtcaatgta cgtccaactc
    11521 cgttgaggaa ggaagcaatg tccatcttag gcttaccagc tggctgcact tgtttgaggg
    11581 atagagcccc ttcagccgtt gcgacaatca attctttctt gcagatagag agaatctcac
    11641 ctggatttcc ctgaccttct actggtaggg cttcataaat cttaaagcgg tcgcccttaa
    11701 ggaaagtatg ggcaacaggc caggggttca ttccacgaat ttggttaaag agttgacgat
    11761 tggttttgtt ccagtccagt ttttcttcct ctggctttat atttggagag aaggtaacct
    11821 gactcgtatc ctgcggttca ggtttgatat caccagcaat ataggcaggc agagtgtcca
    11881 aaagcaaatc acgaccaact agcgccaatt tttcaaacaa ggtgccaaca ttgtcctcat
    11941 ctgtgatcgg aatgctgcga cgagaaatca tatctcctgc atccatttcc ttaaccattt
    12001 ccatgatggt cacaccagct tcctcatccc cttgaatcaa ggcataatgg ataggcgcac
    12061 caccacggtg tctaggaagg agggaggcat gaacgttgac agcaaagtcc atgctatcaa
    12121 ggagtttgct tgggagaaac tgcccaaaag cagcagtcac aattccatct gctcctagct
    12181 tcataagatc ttccatctct ggacttccag ataatttttc aggttggtag atagatagtc
    12241 ctgcttcctt ggcagcctgc ttgactgggg tttcttggat aactttttta cgaccaacag
    12301 cacggtctgg ctgggtcgca acggctagaa tttcgtaacg gttatctgtc aaaagtcctt
    12361 ttaagactgt tgctgaaaag tcgggggtcc ccataaagat tagttttgtc atatcttctc
    12421 cttcttataa aaattgttgc ggctcatggt caatgctgag acggagctca ctattttccc
    12481 gttcttgagt caaggctaaa acctggttga gggtcggccc cagctcatcc tctaaacggt
    12541 atttaattaa aatctggtaa tgatagaggt tgtgggtacg ggcaatcggt tttggcgttg
    12601 gccccagaat gggactggtc tctgacaagc ctgaccgcaa aatgttcatg acttcatagg
    12661 cacgtttgaa aacctcttct tctttcttgt gagaaagggt aataccaatc gtgaaatagt
    12721 aaggtggata gccgagttgt cgtctgattc ccatttcata ggcataaaag ccttcgtaat
    12781 cttgatcctt ggcaaatcga atagcatagt gctgcgaatt gtaggactgt atcaagactt
    12841 gacctgcttt ttcagcccga cctgctcgac ctgccacctg agtcaagagc tggaaggttc
    12901 tctcagaaga acggaaatca ggcagattca aggccgtatc cgcatttaga actccgacta
    12961 gggtaacatt gggaaaatcc aaaccctttg caatcatctg agtaccaagt aaaatatccg
    13021 cttcccctcg cccaaactgg tcaagcaagg cttggtgact gcctttcttt cgagtcgtat
    13081 ccacatccat cctcaaaatg cgagcttggg gaaagagttc tgctagctca tcataagcct
    13141 tctgagttcc cgtcccatag taacgaatac tgtggctctt acagttagga cagacctgag
    13201 gaatatcctt cgagaaacca caataatggc agttcatagt cttggtatcc atatgcaagg
    13261 tcagagaaat atcgcagttg ggacaagtat ccaccgtccc acactcccga cacatgacaa
    13321 agctagaata accacggcga ttgagcatga gaaccacctg ctctttttta accagacggt
    13381 cttggatagc ctctagcaaa ggaggcgtaa agtttgacgt ctcattttgt ccgatatagt
    13441 ctcgaaagtc aatcacttga acctcaggga ttgtagccaa aggattggca cgttgggtta
    13501 gacgtaagtg ttgatagacg cctttgccag cacgtgcccg gctctctaag ctcggcgttg
    13561 cagatccaag taccagagtt gcttgattat actgagcccg taaaatagct acctctctgg
    13621 catggtaacg gggattgctg tcctgcttat aagccgcttc atgctcttca tcaataatca
    13681 tgacacccag atttttcaga ggagcaaaga tagcagatct ggcaccaaca acaacttggg
    13741 catcgccacg ctccaccttg cgccattcat catacttttc gccattggat aatcctgagt
    13801 gaagaatggc taccttgtcc ccaaaacgtg ctataaaacg ctcggtcatc tgaggagtca
    13861 aggaaatctc aggtaccagc aaaatagctg tcttgccctt atccagggca ccttggataa
    13921 tctgcaagta aacctcggtc ttcccacttc ctgtaatccc ttgaagtaga aagggaggtt
    13981 gagaactgcc aatagaactc acaaccgcat cacgcgcctg tctttgttct ggatttaact
    14041 ccaaaggtct acttgcttca attccttcaa aataagcagc cgagcgtcga acttcctttt
    14101 ggactatggt aacagcacct tgatccacaa agaagttgac ttgctctcgc gagtaggact
    14161 ctaacaagct agccaaggaa gcgctctctg gatgagacag cagataatct ctcagttcca
    14221 actttttctt ggcacgtgta gaaatctcaa caccttctaa ttgagcatgg tcaacctcat
    14281 accaagactg ggtcttgacc ttcttttgat cgactgcctg atattccaga ccaagcaggc
    14341 cttttctagt caaacgcatc atttcagctt gcttacctaa atccagagaa gaaaaggcta
    14401 gcgaatcttc tgaaccaaac aggcgcactc gttcttcctg actcaagcct tccagaggat
    14461 agagaatctt gtcatagcta gaattcagaa atcccggaag catggccttg aggatagaga
    14521 ttttgtagga gaagacagat ttgcgtaact cctcagccag ccagagttgt tctggcgtga
    14581 gaacaggaga aaaatccagc acctctgcaa tatcttttaa atcttgctcc atctcttctc
    14641 catctgattg ggactccaaa ccaaaaacaa tcccttgaat caggcgatta cccttaccaa
    14701 aaggcacatg aacccgcatc ccaacttcca gcattccctc aaattcctcc ggaatcctgt
    14761 aactataggg ctggtccgtc tgcatcaagg gcacatctac gataatctta gctagggcca
    14821 tcttctcacc tcctccttgt cagtacattc ttgcaataga aaaaataaga ttgagtcccc
    14881 ccaaccttaa attttttcac catcttcttt ttctttagca atttgctctt tgattttctt
    14941 ttcttcttct tctttgcggc gtttttcttc ttcgatacgg cgacgcactg cttcacgttt
    15001 tccttctgga tctgggtgaa ttgtaacgtt tcctgattcg atttcttcta aagcgcgaag
    15061 agttgatttt tcagacttga aaccttgagt tgctggggca cctgcttcca attcgtgggc
    15121 acgttttgct tccaagatta cgagtgaata ttttgaagga accttgtcga gcaaggtatc
    15181 aatagagggt tttaacatca tttgcttgta cctattttct aaattttatc gggtagttgg
    15241 agattttggt aacatctcct gatagtgacc aatgacacga tccacacaga agtgttctgc
    15301 ttcaatcaca catttgacac gttcagcagc taggggcacc tgatcgttga caatcgcata
    15361 atcatactca cgcatgaggg caatttcttc cttggccttt tcgattcgtt gggcaatcac
    15421 ttctgcacta tctgttccac gacctaccaa gcgatcttgc aattcatcca aatctggtgg
    15481 tgtcaggaag ataaagacag catctggaac ctttttcttg acctgaagag caccctgaac
    15541 ttcaatttca aggaaaacat cgattccctt gtccaaggtt tcattgacat aggtcagagg
    15601 agttccatag tagttgccga catattctgc gtattccaac atctgtcctt gacgaatcag
    15661 ctcttcaaat tcttcacgag tacggaagaa gtagtcaaca ccgtccactt ctccaggacg
    15721 ttgtgcgcgt gtcgtcatcg atacagaata ttgaaattgg ttttcagaac tctcaaaaat
    15781 ctctcttcta accgttcctt ttccaacccc tgaaggacca gaaaaaacga ttagtaagcc
    15841 tcggtctgcc attgtgtctc cttttagtca atctgtgaaa taacatttct ctagaataat
    15901 ggcaaaaagc cagattatcc tttacagtct ttctatctag tgtaacaaaa aagcagtaat
    15961 ttttcaactg ctctttctta tttatttagc ataatctact gcacgaagct cgcgaatcac
    16021 ggttaccttg atatttcctg gataatcgag attgttttca attttcttac gaactttgtg
    16081 agccaagatt gtgactttgt cgtccttgat ttttcctgga ttgaccatga tacgaatttc
    16141 acgtcctgct tgaagggcaa agctagtttg cactccttca aagccgttag caatttcttc
    16201 caaatcatgg agacgcttga tgtagctttc aagagactca ctacgagcac ctggacgggc
    16261 tgcgctcaag gcatctgctg cagcgacgat aactgctatc acgctctcag cttcaacatc
    16321 tccgtggtga ctagcaatcg tattcaccac aactgggggt tccttgtact tacgggccaa
    16381 ttccataccg atttcaacgt ggctaccttc aacctcatgg tcaatggctt tcccgatatc
    16441 gtgaaggaat ccagcacgac gggcaagagc cgcattttca ccaagttcgc tcgccatgat
    16501 accagccaac ttagcaacct caatcgaatg gcgcaaaaca ttttgtccat atgaagtacg
    16561 gaactgcaaa cgtcccataa tcttcatcaa gtctggatga aggtttggcg caccaatttc
    16621 ataggcagca gcctcaccgt attcacgaat cttattgtca atctcttgac ggtttttctc
    16681 aaccaactct tcgatacgag ctggatgtat acgaccatct ttgagcaaca tttccatagt
    16741 catacgggca atctcacgac gaatcggatc aaatcctgac aaggtcacca cttctggtgt
    16801 atcgtcgata atcacatcga cccctgtcaa actttcaaag gtacgaatgt tacgaccttc
    16861 acgaccaata atgcgtccct tcatagtatc gtctggcaga tgaaccgttg agtttgttga
    16921 ctccgctaca tattcaccag cgatacgttg catagcttga accaagatgt ccttggccat
    16981 tttgtcagaa cgttccttga cctcttgctc agcttcgcga atgcgactgg caatctccct
    17041 ggtcaagttt tcctctgtct gagccaagat aatatctcgt gcttctgcct gagacagagc
    17101 accaatacgc tctagttctg cttctttttg tctttcgact tcctctaatt gctcttcacg
    17161 cgcatcaagg tttttcgctc tatcagaaat actttgttct ttttgttcaa gtgtttgttc
    17221 tttactcgtc aaattgtcgt ccttacggtc aaggctagta gctctctctg tcaaacgact
    17281 ttcgatttgt ttgagttctt gacgttctga tttgaattca gcgtccactt cttcacggta
    17341 ttttctggct tcttctttgg cctccaatag tgcttctttt ttaagagact tgctttcacg
    17401 tttggcttca ttaacaagta aatccgcttc acgctcagct tgtccacgta aattagttgc
    17461 ttcttgttca gcatttaaaa gcatcaactc tgcagcttcc tgagatgatt tcatcttagc
    17521 tgagatgctg acatatccaa tgactaaacc aatgatgacg gcaaaaacag caatcgcaag
    17581 cgacatgatt tccatgtttt tacctcattt tattgttatt ccgaatgaca tacattcttt
    17641 tacattctac cataaaaaag tgattttcac aaacctaaaa tagaatatgt tttggggaat
    17701 ttggaacaca tttaccaaaa taaacttgtt gtttagaaat agtagtttag tagagacttg
    17761 agaaaaagcc tactttgcag tagacttagt aatgatcttt aaaggacaag aaagccacgc
    17821 tatctccatc catcatataa atcaagcgat tttctgcatc aatacgccgt gaccaggctc
    17881 cttggtaatc atatttgagt ggctctggtt ttcctattcc tgtaaatgga tcgcgttgaa
    17941 tatccttgat taatttattg attcttttta gtgttttttt atcttggttt tgccagtagc
    18001 aataatctgc ccaggcatct tctgtaaact tgagcagcat ttcttactcc tcaataacat
    18061 ggacctgagt acttccagca cgaacttgag ccattcctcg caaaacctta tcagaaagtt
    18121 ccttattttg agcaattctc agggtttctt ggatactatc ccactcactc tttgaaagga
    18181 ctacaatgtc ctcatctgga tttttattga ccaccgtcaa aggctcaaat tcatcattta
    18241 ccttcttcat gtagtccttt aaatgatttc ggaatgttga gtaaaggact gcttccataa
    18301 ccatacctcg ttttagctct tttccactat tatacacgaa aagaaagaaa ttgtcaggaa
    18361 cttgtacaag attttctttt ctatctattt atactcaatg aaaatcaaag agcaaactag
    18421 gaaactagcc gcaggctgta cttgagtacg gcaaggcgac gttgacgcga tttgaatttg
    18481 attttcgaag agtattattc gtaaaaaatc tcaaaaagtc cacctttcgg tagacttagt
    18541 ttgtttctat tctaatcggc actcttccaa aattctgttg tgctatactt ggctttccta
    18601 gttggtaaat ctggtcagcc ttttgagtca tagcatccca aggttctttg ccaattcggc
    18661 tgactagact gacctgacct ttcaaagact tgagatgttg cctacctttt tcagtaaatc
    18721 caaggacatg aatggcttct ggcaagtcac tttctctagc ctgcatcaaa atataggtca
    18781 ataggcgtct gacacgcgcc ttggtgtaac gtttggtagt cactaactcg accaattctt
    18841 ccacagactg ggctgtttta atagcttctt taatgcgcac agccatttct tgattgacct
    18901 gatagatggt ggttaggtcg ggatttgaca agatttgata gcggagcaag ggaaaatagt
    18961 cttcccagat caccttactg gcctgctcaa atagggcaac agaaggcata aagcgttcta
    19021 agaaatcttg gtccttctga tgttgacgga gggctgtcgc cgaggcaaag tccacatctt
    19081 tattcacaga atggtaacct gccccctgac gctgaatcgg atgcagcttg atatttcgtc
    19141 ctgcaaccgc ctttgcatag gccagagcaa gaacatgatt gggggtatta cctgaaaaat
    19201 caagaccagc aaattccttc cacatagctt gggttttctg gggataggaa agggaatcag
    19261 gcagattttc cacaaatttc tccatctcag tacctttctc tgtgtataag tcagcgattt
    19321 tctgataatc tcgaacttcc tctgtcccaa aaactagact atcaatgccc aagcgatcca
    19381 agatatccac agctccttga ccaaagaaat cagctgcctg aacactgact aaaaagggca
    19441 attccactac caagtctgcg ccattttcca gcgccatctg ggcccttgtc cacttatcca
    19501 caatagcagg ctctccacgc tgcatgaaat tccctgacat ggcaacaatt ttcagtccct
    19561 ctgcctgatc cagcaggtat ttatggccat tatgaaaagg attgaactct gcgataatac
    19621 ctgtgatggt catgatgttc tcctacttct gcgccacaaa aaaccaacgg gtgctagttt
    19681 ctgttggttc cttatcctca aagtctgcat agagtttgaa ggacttaaat ccagcctgtt
    19741 ccagcaagat atcataggtc aagatctcat aggtcctctc ctcatgtact tcatcgtggc
    19801 gactaaagga accgtcagcc tccttgataa agaaagtcag ctcatgcacg atggagtgag
    19861 gaacttcgtc ctcatatgta tcccaaagca tggcaaaatc ttccgcattt tcatggtagg
    19921 aatagccagg gaatacttca tctgtctggt aggtcgagtg cacgtcaaag atgaaaactc
    19981 cctcttcatt cagcacattg tacacgtcct taaagacgtc ccctacttcc acctcatcct
    20041 gcatatagca gatagagtcc gaataacacg tgacaaaatc gtatttccct gccttggaca
    20101 aattcagcat attaccttct ataaaagcaa tcttttgctt ggctgaagtt gctctcttct
    20161 ccgcaatctt caacatatcc gcactcaagt caagtccagt cacatcaaaa ccagcctgag
    20221 agaagcgcac tgattgaatt cctgtcccac aagccaattc caagagtttc tttctctcct
    20281 tggtcttagg caaatgacgc agagaaaaat tcgtccattt gtcgtataaa ctatcgtcca
    20341 tgaccgcatc atagaccgcc gcaaaggttt cataagtcgc cataatcatg ataccaagag
    20401 ctccatggta aacaccacta gccctttacc tttctatcaa tttttctaaa ataagcaaag
    20461 aaacccagtt gactacaact gagttcatct tctaagcaag agcttctgaa atatctactg
    20521 aattcgcctc atgccatagc ttctctagat tataatgggc acgcatttct tctgaaaaga
    20581 tatgcacgac aacagcaccg aggtccagta agacccagcc tccagctgca tcgccttcga
    20641 catggctacc tttaaagcct gcttgagcta ctttttcacg gatattagct gcgatagcgt
    20701 ccaactgacg gctattcatt gagctagtga tgacaaagta gtccgtcaca ctagtcaaat
    20761 cttgtacatc aagtgcgagg atatcctccg cacgtttctc atcagccgct ttcacgacta
    20821 gttctagtaa ttctttttcg ttcatttaat cctcttttta gaaaatatgt ttgtaatcta
    20881 ggacatctgc aaagcgctcc tcccaaatgt cctcataaaa ttcattaatc gtttctgctg
    20941 gaatgtcatc cccatcaaag gttgtgacag caccttctgg aacaataagc tgatagccat
    21001 attcaaaggc aaccttgaca gaggtatcca cacaatattc tgtctgcata ccacataaaa
    21061 ctaatttttc aatcccctgt ttatccaagt attcttttaa gccagtttct ttgaaaatac
    21121 tgttatactt cttctgaaag accttttcag caggttttcg atttaaaagc gcagataact
    21181 gccaaccttc tgatgtttga gcttcagagt tctcaatatg ttgaacatag ataatttcaa
    21241 tattcttgct tctagcctgg ttttgtaaat aagaaatttt ttccaataga ctttttgtct
    21301 ggaacccagt ttctactaaa acattctgaa catcaatgat tataaaagcc gtcttcattt
    21361 agtcctcttt caaatagtgc acaaaggcgt tataggtttc aagggtttgg ggatagatgg
    21421 ggaatccctg atgagctaga tactccacgg tacgagctgt ttcgtaggcc accgccttat
    21481 tgagcgatag acttgcaatc tcacgcgcca catctactcc aggaaaggat cgattgtgct
    21541 cgatatagtc tgctacatag acaaccttat ctaggtctgt catctgacca gcaccgactg
    21601 tatggacttc aatggctcgc aggatttctg aatcatgcaa atccaaatct tcctgaattt
    21661 tgtaaattcc gaccatacca tgccagacat tattgcccca gtttttgagg tcagggtcta
    21721 gctggtaacg gtcaatcaag tctagaaatt cctgatctga caacttttta gcatagtcat
    21781 gaagaagacc tgctagactt gctttctcaa catctacacc aaagcgctga gctaattcca
    21841 tagcggcacg ctccacacct agacaatggg ttagacgttt ctcaggtaga agctctgcca
    21901 ttttttccaa caaggcctca cgagagcagt tgatatagtc ttgataggac atcagtagag
    21961 cccctccttc tcgatatagt ctagcacaag ctgaggtagg agaaagttgg gtttccgacc
    22021 ttgggcaagg aaggcacgca ccatgctgga cgagatatcc atgagcggta cgtccaccca
    22081 gataactgga taggaagtcc ctgccttgta gcgtggacgc tgaaccccca caaactgaac
    22141 catgtcaacc agttcatcaa ttcggtacca cttaggcaga tagtcaacca tgtcggcacc
    22201 gatgataaag taataatccg tatctggatt cttctctgtc aaaatcttca tggtgtcgta
    22261 ggtgtaggaa atacccttgc gctccaactc aatggtttca atgactaggc cgtcaattcc
    22321 ctcaattgcc aactcaagca tcttgagacg atggtgttca gggatggttt cctttttatc
    22381 aacgtgagga ggttggtatt caggcatgag cagaacttga tccagtccca actgttgccg
    22441 tacttgatcc gcaacaatga gatgggcatt gtgaacaggg ttaaaattcc cccctaaaat
    22501 cccaacttgt ttgcgttttt tctccttgat ttctggctcc aactctacct tggtaaaggg
    22561 agtcaatagt tcgattgtca taggctagtc ccctttattt ctctataaaa acagttattt
    22621 ggagtttcgg tcttagattt ctttgacttt cttagaaatc ttgcgatttt ctttcttgct
    22681 agattgttta aacaaaatca agatgcgtcc tattttttgg actgtatcca caccgatttc
    22741 ttcttccaaa atttcagcta cttcgtggat gttttcatct gtgttttgta agagagtaac
    22801 cttgattaat tcacgcgcat caagagcttg acggacgctg gttttgattt ggtcgttgag
    22861 tccatttttc ccgatttgga tgataggttt gagggtgtgt gcctggctgt tgaggaaggc
    22921 acgttgtttt gatgttaatg acataatttc tttaaaagag tttcttttta tacttttctg
    22981 aagtggtgac ggacgtcagc aaagtccttc ggactttcat gactaaaatt tgagcctaag
    23041 gtctcaaatt ttccgcagtt ggtacaatca cttgtaccaa cttacaccac agcgaaaagt
    23101 attctctatg gggctcgcct tgctcgccca aattcagaca accctttcct ttctgtgctt
    23161 aaataattgc ttttcgtgtg acgacggcga cgccttctgg tgcccagacg gcgacttttg
    23221 ctatgcctgt tacacgaatc cagcctaggc ctgagatgac taggtctgtc ttatctttaa
    23281 tggtaaagac atgctggact agccttggga aatcttcttt ttccttgcta tttggtggtg
    23341 tcagaagagt tcccaggtgc ttatcgtaga aagcactagc tccttcaagc ttgctacgat
    23401 ggagtttgag ttcattatca aagaaagcag taaatccttg cttttctcct gctataaagt
    23461 caaagcgtcc caaaccacct aaaaataggg tttgctcagg attaagctga taggtcttag
    23521 gcttgatttc ctttttagga ctgacatact tgaggttttt ggccgtcaag tagtgagcca
    23581 tctggtggcg gtggataatt cccggcgtat cgtaaatata agatccgtcg tcaagcggaa
    23641 tctctatttt gtccaaggtt gtccctggga agcgtgaagt agtgatgaca ttctgatcac
    23701 ccgtgatttc ttggataata gcattgatta gagttgattt tccaacgttg gtcacaccga
    23761 ccacatagac atcgcggccc ttacggtagt gttcaatctt gtcaatgact tccttaatgg
    23821 catgtttatt ttgtgctgaa gttaggacca catcgactgg acgaagacct tcttcatggg
    23881 cacgtttcat gagccactgg ctaatcttac cagacttaac tgacttaggg aggatatctt
    23941 ttttatttcc taccaagagg acatcattgc ccgagacgaa acgtggtaaa cctgggatga
    24001 cagagccatt aaaatcaaag atatcaatga cattgaccac taaggcatca ctgtctccca
    24061 cctcgtgcaa gagcttgagg aaatcatcgt tcgtcaactg gacatctgtg atttcattgt
    24121 agtggcggag acggaaacag cgttggcaat agacttcgcc agtctccaaa cctttttcaa
    24181 gtgccgactg gggggtaaaa ccaagaccag ccttatctgt cgtctgaatg gttgctccac
    24241 aaccaataca gagaatttct tccatagtta aattcctttt ttatatgtaa tcggtccgta
    24301 cttttcagtg atttttctca taacacgacg ctcacgagtt cggttaatct gcgttttgat
    24361 tgagtcatgt tggaccaagg gtttgactaa aattgaccga atccctgcac ggtgggctgc
    24421 tcgtatatct gtcatgagct ggtcaccaac catgaccact tcctttttgt catagtggaa
    24481 ttccttcata gcacggtcaa taccaaatgt gaagggcttc aaggcccagt aaacgtaatc
    24541 aatcccaaat ttctcaactg ctcgttgaac gcgttttttg gtgttatttg acactacgat
    24601 aatgccaata cccgcgtccc gaaggtcatg tagccattgc ttcatctctg gcgttccatc
    24661 agggttgttc caagcaatga gggtattatc caaatcgacc aaaacagcct tgattccctg
    24721 cgcctgcagg cttgggactg tcagatcata gactgcttcc acagcaaaat ctggtatata
    24781 attttcaatc gccattctgg ctccttttct taactttttt tcgcaatttt ccttagtaac
    24841 aaggataaaa caatccacaa acctgcacta gccaaactaa tatagagcca agcatggggc
    24901 tcatctgtta aaggtaaggg aacattcatt ccgaaaaagc ctgtcacgac tgccaaaaca
    24961 gctagcaaga ctgaaataat agtcaaggtt gtcaaattgt cattcagatt attgtttaga
    25021 atattgttgt aagaggctga aagctgctgt aaaatctgag agattaggtc tgtcatggat
    25081 accagctgat gagcctcaat catggcatca tcaaactgtt ctctctcaat ctcatcaaaa
    25141 ctacgataca aggcatgacc ttgaatatgc tctaacaaaa tccgattttg tttggcagct
    25201 gccgtcagat aaaccatacc agtctccaaa tcagacaggg caaagaggtt tttcttggta
    25261 gttcgctggc gcaagaggtc attgacctca tccctactct tgtccatctg ctcaatgaca
    25321 ggatagtagg cattgctgat gatttccaga ctggcaaaga gaaacttata aatcgaaagc
    25381 gtgtcatggt tctccagata acgagtcatc tgttcaatga cataggcgtt cttggtatta
    25441 ctaatggtaa tcaggcgacg atgctcgacg ataaaggtca tgggaaaagt ctcatagtag
    25501 gccttgtcct tttttacgtc taggacatta tagataaagg taaccgtccc actttcacgg
    25561 tggtagtcca tgtgggcgcg ctcgtttcta tccagtgcgt attcaatggt ttccttgtcc
    25621 aaaccgtaaa tttcagaaag gtcttctagt ttattcaact ttcctaggtc taggtctatc
    25681 caggtacaac cattgcccaa ctgcttttct aaaaccatct tttctcctta ttaaactccc
    25741 tctattgtac cacaaattac ttaaaataac aggtctagtc tgtgcgtgag aaattgctaa
    25801 caactgtgat atttcggtta gggacaaagt gaagggcttg ctcctcgcta cgaagctgtg
    25861 tcgtacgaat tcccacactg acaaccttac ccgatacagt aatcggtcca tttgtcagaa
    25921 cgacctcatc tcccacatcc agttgacgtt caaagaggat gaaaaagcca ttgatgacat
    25981 cagatagaaa gccttgggct cccataccaa tcgctacccc agcaatacca gctccagcca
    26041 gcaaactaga aactggcaaa cctaaaatcg acaaaatgca gtagagtaaa aagaaatata
    26101 gcgtataatt aaacacattt tctagtaaac gtgagatggt tttttggcgt ccgacatcat
    26161 gacgagacat ttttagagaa ggtttgacaa ttctctgcac catggtatga agcatttttt
    26221 tagcaatata aaatacaatc aaaagcagta aaagagaaat gaccttggtt agaatattct
    26281 cgataatggt tgtcacatct agcttttcaa tataagcctg aataaatttt tgcatataaa
    26341 actcctttat ttcattatac catattttca tcagtctcat tctccataca tattcaaaaa
    26401 atagacagaa aattcttgat tttgtctatc atttatacta taatcataat cactacacaa
    26461 aaaggagatt attatgaaaa gaatcattca tgcttggaat aaggcaagcc ttgtcaagcg
    26521 tatcttgatt ggtatgcttt atggggaatc ctaggactga cccttcctaa tctctcaggg
    26581 attgggctac tcggggattt attcgttgga ggtctaaaag ctgttgctcc tatcctagtc
    26641 tttgccctcg ttgccaatgc cctttcccaa catcaaaagg gacaagatag caatatgaaa
    26701 actgttgttt tcttgtatat cctatgatag ggactttcgc cgctgctctt gtagctgtac
    26761 tagcaagttt catcgtccct attgaaatta ccctaaatag tgccaatact gaaattgcac
    26821 caccagatgg gattgggcag gtcctcagca acctcttgct caaactggtt gacagcccag
    26881 tcaacgccct gcttactgct aactatattg gaatcttatc ttgggcagtc atttttggaa
    26941 tcgctatgag agaagccagt aaaaatagta aagaattgct aaaaactatc gctgacgtga
    27001 cttctaaact tgtcgaatgg atcatcaatc tgactccatt tggaatcctt ggtcttgttt
    27061 ttaaaaccat ttctgacaag ggagtcggaa gccttgccaa ctacggtatt ttattggttc
    27121 tattagtaac gactatgctt tttgttgccc ctgtggtcaa ccctttgatt gccttcttct
    27181 ttatgagacg caatccttac cctctagttt ggaactgcct ccgtgtcagc ggtgtgacag
    27241 cctttttcac tcgtagttct gcggctaaca ttcctgtcaa tatgaaactc tgccatgacc
    27301 ttggactcaa cccagatacc tattctgttt ctatcccact cggttctact atcaatatgg
    27361 ctggagtagc gattaccatt aaccttttga cccttgttac agttaacact cttggaattc
    27421 ctgttgactt tgccacagcc tttgtcctca gtgtggtagc agctatctca gcctgtggtg
    27481 cttcaggtat tgccggaggt tccctccttc ttatcccagt tgcttgtagc cttttcggta
    27541 tttctaacga tattgccata caaattgttg gggttggttt tgtgattggt gtcatccaag
    27601 actcatgtga aacagccctt aactcttcta cagatgtcct ctttaccgcc gttgccgaat
    27661 acgcagcaac ccgtaaaaaa taactcatca aggcaagcct gctcatgtct tgtcttttac
    27721 gcttttattc taacttatta ggaaattctt atgtctatta gccaacgtac gaccaagctc
    27781 atcttagcta cctgtcttgc ctgcctgctt gcttattttc tcaatctttc gtcagcagtt
    27841 tcggctggaa ttatcgctct cttgagccta tctgatacgc gtagaagtac tttaaaactg
    27901 gctcgcaatc gtcttttttc tatgcttcta gctctggcta tcggtgttct agcttttcac
    27961 ttgagcggat ttcatatctg gagtctcggc ctctatctgg ccttctacgt tcctttagcc
    28021 tacaagatgg gctgggaaat tggcatcaca ccaagcacgg ttttggttag ccatctcttg
    28081 gttcaagagt caacctctcc agacctccta gtcaacgaat tccttctctt tgctattggt
    28141 acaggatttg ccttgcttgt taatctctat atgccttcac gagaagagga aatccagcac
    28201 taccacacgc tggtggaaga aaagttaaaa gatatcctcc agcgcttcaa atactattta
    28261 tccagaggag acggacgcaa ccgagcacag ctggtagcag aattagacac gcttttgaaa
    28321 gaagccctca gactggtcta tttggatcac tctgaccacc tctttcacca gaccgactac
    28381 catatccact actttgagat gagacagcga caaagtcgta tcctgagaaa catggcccaa
    28441 cagattaaca cttgtcacct tgccgccagt gaaagcctga tcttagcgca actcttttca
    28501 aaaattgcag gtcaactgag ccagaccaat cctgcttctg atttgctaga tgaaattgaa
    28561 cgttatctgg aagtcttccg gaaccgcagt ctgcccaaga caagagaaga atttgaaacc
    28621 cgcgccaccc ttcttcaact cctacgtgaa gccaaaacct tcatccaagt aaaagttgat
    28681 ttttaccaaa aatatagaca gtaatactct tcaaactacg tcagcttcac cttgccgtat
    28741 atatgttact gacttcgtca gttctatcca caacctcaaa accgtgtttt gagctgactt
    28801 cgtcagttct atccacaacc tcaaaaccgt gttttgagca gcctgcggct agcttcctag
    28861 tttgctcttt gattttcatt gagtataaaa aagaaaactt cagaccaatc cacaaaaggt
    28921 gaatatctga agttttcttt tatttataag taatcaccat aaaggttaat agtagaacaa
    28981 ataaagccaa actcaccaat aaagtgagca ccttgaccaa ggtgttgctc tgccagtagc
    29041 ttggtttacc aatattacta gtggcatcca tggaaaatag ttgattttga cggggttgta
    29101 tagttaccaa tacaatcaaa gcaagagata ggataatagc taaaacaatt agtatgtcaa
    29161 tcatagatct acctcaaaat tcccagcaaa gtaaagagca aaccaatcag aatcatcaaa
    29221 cccagtaaaa gtgaaaaagt cttactaatc ttttcccctc gggtttcttt ttccttcttg
    29281 atgggttttt gtttcaactc ttccatgcgg ccttcaacgt ctttgtaaaa taaatcttcc
    29341 tctgacatgt tagcctccta aaacagtttg catggtttcc tgatatctca ccaagtcaac
    29401 ataatttgca tgttggcctt gtttgccaag tgcctgacgc atttgatcaa catctgaaag
    29461 ggccaattta atggtctcaa ccaaagcagc aacttcacta ctttcaaata gatggtctgg
    29521 agcgacataa agtctattgt gcaccgtctg attaaagcca agaatcaaga gattgtgctc
    29581 aaaggcctga cgcactgcct gtagcaactc attactgtgg tttatatcca agtaaatatc
    29641 cgacagttga tacagctcct gaatcttctg tggactagca ttctggtaaa gggccacatt
    29701 aggatagcaa agcatgtcta agagcttaga agacatctct gtcactgctg cgatacggaa
    29761 ggtaacatca ggcaaggctt ctacgattgc ttctacttgc tcaatctgat ctgaattggt
    29821 caagattaag gcgtctcgtc ttaggaaatt atcacgtttg aactggtaat gataacccaa
    29881 gtgcacaaac tgatcatggt atttctcgtc agttaactct aaagcgcgct cataagtcgt
    29941 cttatttgga atgatgatct tcttagtacg cacattatca ctttccaaaa tcaactgcat
    30001 attacctgga atggcatcat aaagaggttc ctgccatacc aagacatccg agccagattt
    30061 atctggataa tgaaaggaaa ccaagaaagg agtcgctaga gtattaaaga taagctgact
    30121 ggtgtctatt tccaaatctt gcaaaaagaa ggtgataaat tcaactttat ttgcaaagta
    30181 acgcatggac tgaccaggca aagtcaataa gatatcaccc gtcacatggt tttccagtaa
    30241 aacttgttga ccattgacat cttggtaaac tgtcataatc ggctcgctat ctgcgctata
    30301 agtggtttta gcaaaacaag ttccgaaacg attatagtgg tcaacctgac gtactcgacc
    30361 ttctaggtct ttccagtcta cctgtttaac caagcgagcc tgcattccat cagcataatg
    30421 aatgacagcc ctctcctgcg tcacatcttc aatacaagca gactgattat ctcctaaaat
    30481 ttcccaaaaa tctgaaacgg gaacttgatt aaaatagaga ggttttccat cctcgtaacc
    30541 tagataatag gtaaaaggag aaagcagacc atcaggcaga aaaccatctg catcgatgac
    30601 cactccaagt tgagaaagac cagtagcgac tagactttca tgtaaatctc gactttcatg
    30661 actataacga tcataaagtt caatcatgga gcacctcctc tactgttttc ttccactttt
    30721 ctaaaatttc tttggtcaag aagccttctg caatttggta agaataggca cgcatagctt
    30781 ccaaacgatt ttcttgatac aattgacaaa tcttagcggc ataagcttgc ttgatttggt
    30841 cttctacatg gtcagatgaa cttggaatca aataaccatt ttgcccatcc tctataaagg
    30901 tctgattacc ataaggcaca tcaaaaccaa ttagaggtag acctgaacca atagcttcca
    30961 tcaaggtcag accaaatcct tcgctggtag aagccgttaa gtagacctca tactggctat
    31021 aaatctgcga aagttccgca tgccccttga gttggatata gtcctctgcc tgatgatttg
    31081 caataatgtc tctaagcaga gaatcttctc caccactacc atagatatca aaggttagtt
    31141 ccggtaactc cttatgagct tcaatcacag ctttcacaag ccaatcaatg tgcttttctt
    31201 tggcaagacg tgaagccgta atcaatgaaa atggtttgcg cccttgactt gaatctgtca
    31261 aggaatcaat actgcctaca ggaatggtaa caatctttgg ctgatgctga gtatatttgg
    31321 caaattgctc ttgtagaact tcattttgtc tatcagtaga cacgataaag aagtcaacct
    31381 tatctgcatt ggtaaactga tagtcataat agttattcca aaggatgtag tcctcatttg
    31441 tagcattttc actataatgc tccgcatgaa caactaccgc taaatgtgct gtctgtgctt
    31501 cctcaaacac aacctgacca atgcctgtct ccctatcgag aatgaccaaa tcagacttat
    31561 tcaaattcaa agatttcata aaggcacgca caaaagcttg ctttccatag aaaatcttat
    31621 ccttgaaatg ataaacttct tccttccctt gattcatcaa gatatcatag actggagtcc
    31681 cgtcttcatt ataaaaagtt cgttggtata agactgcaac attgtccttg ggagcaaaat
    31741 actcgctaca ataacgcgta taagaaaagt aatccttccg aatcaggttt cccttaaaaa
    31801 catactcggc atgttgaacc aagtccttgt tctcatcaac caaataacag gttacaaact
    31861 tatcttggtc aaaaaagaat acacgtaaaa ccttgccatt tttttctctg tgactttctt
    31921 caccaccaaa gtaagccaag acatcatcca ctgtcacgct agtaggtgca attttgatat
    31981 ctgtgaaatg attataaagc cagataacct gattatcatc aaaaccaata ttggctgtta
    32041 agtgctgaat attatcggct aaaatcatat ctgtaaagat aaacttagag gacagattta
    32101 atttccgaaa aacaccagca cgataggctt gagcgtattc aacaccgcta ctagcccaac
    32161 caattcctaa atttatattg taaattgtca tattttctcc tcttatggaa aatgagtcac
    32221 gatttcacct tttggagtga cctcaaccag ccccatcagg agattacgca ccatatccgc
    32281 acggatttgt tctttcatag cttcaaagcc cgcgtaggct tcttgatagt actctacgat
    32341 tggatttttc tgactagcag attgaccacc gatagccatg gatagctgtt gtagatagtc
    32401 tacctgctct acccagttgt catcaatggc tttaagcagt gaaagtcgta aaaactgttc
    32461 atataagtca tgttgattaa gtaattcttt cttttcagaa agttctttat caatcacctg
    32521 cttcataaag ctacgaactg cagttttgtc agttacatct atataatctg gaacctcttt
    32581 aacatgaaaa ctaatattgg tcacaataaa gtgaaacaat aattcacgac tagcatagtg
    32641 atcagccgct acctcttctg tatatctctc aatgatatcc acaacaacat cctctaagtc
    32701 acgagaacca tctattagac gatttctctc tttatagact atatcccgtt gtatattcat
    32761 actttcagca tactccagag tctgacgacg tgctgaacgt ccagcactat cactggcatg
    32821 ctgagccttt tcgactagtt tccggtattt acgacctttc aatacttccg gttgagtcat
    32881 atcttgaacc tgatagtctt tgtacttttt atgcacccaa gatggaccaa atttcttgat
    32941 aacatcatcc tctaaggata caaaaaattt actcatacca ggatctccct gacgaccaga
    33001 acggccacga atttgtaggt cgatccgctg actttccatc cgctcagtcc caataacaat
    33061 caagccccca agctctgcga ctcctttacc aagcttgata tccgtaccac gtcctgccat
    33121 agaggtagcc actgtcacag cccccatctg acctgactcg gagataatct gagcctcacg
    33181 cgccgcatta ttagcattta ggacattatg ggcaatccct tcacgaaaca agagagacga
    33241 atagagttga gacatttcaa ctgagcctac aaaaacgagt aagggatttc ccttagcatg
    33301 gtattgcttg atgtactcca aggatgcata cactttttca ggtaaagtga tatatagatt
    33361 atctggatag tcaatccgtt gtctcggacg attggttgga atgcgtacta cagacatatt
    33421 gtaagtttca ataaactctt tttccgcgac cttacctgtc cctgtcatac cagatatctt
    33481 attaaacatc ttaaaaagac tctgataggt gatcgaggcc atagcccgcg tctcaggaga
    33541 taatttgaca tgttccttgg cttcaatagc ctgatggaga cctccttgaa gtttagtcat
    33601 ttccattaga cgccctgttc ccttatcaac cagtaccatc tcatttccac gaatgatata
    33661 gtccttatct ttagtaaaga gcttatgagc tcgaatcgca taaaccaaat gacgagcaaa
    33721 agacgcatgc tcttccttgt ataaattatc aatccctagg aaattttcag cagacttggc
    33781 ccccttagta gtgagccaaa cctcctcttt ctcctcttta aagatataat cctctccttc
    33841 gaccaaggtt gttacaagtg tatcaatgat cgcatagtaa ttagactgaa cacgaggaga
    33901 acccgcaata atcagaggag tttgtgcact atcaagcaag atatcatcaa tttcatcaat
    33961 aatcacatag ttaaacggtc gtaaaaactt accttcttca ttcgaggcta ggttatcatt
    34021 tagataatca aaacctaaat tactattggt tgtgtagatg atatccgaag catagataag
    34081 cttcttttct tcagctttca tttccttctt tggatcttcc gtaaatggta caccaatggt
    34141 caatcctaga aaacgataaa cttggcccat ttcctcggca tcacgctttg ataaatactc
    34201 attaggagtc acaaccatca ctccttctcc tgaaaaagcg ttcaaataga caggcatggt
    34261 agctgtcaag gtcttacctt cccccgtatt catctcagca acatttccat agtgcatgac
    34321 aatagctccc atgacttgaa catcataggg gaacatccct aaaatccgct tatctgcttc
    34381 acgcaccaca gcaaaagctt caaccaaaat atcgtctaga ctttctccct cggaaagacg
    34441 ctgacgaaac tctactgttt tagctactaa ttcttgatcc gaaagagagg acatcttgcc
    34501 tttcagggca ttaatctgct ttaaaatctt tttcactttc ctaagctgga aatcttgacc
    34561 taaacgtcta aacacttctg atctccttga tagaaaaatg ataaaaagtt aaggactcaa
    34621 atccagcact taaaagagaa acttgataag tataactatc ttctggataa gtaaatatga
    34681 aatctttcgc tttttctacc tttttttcta aaatatcacc atagcggtca aaaaaagtta
    34741 tcttggtata aactccattt tcaggctgac aatcaaaaac cagtgatagt tgatactcct
    34801 gctttctttt taataaggga agctgaggtt gggttcgtgc agcctggtaa ttgacactag
    34861 aaaaccaagt ctttagaatc tcaccagatg gcaatagagg attatacaga cgaacacttt
    34921 tgtcagggta ataagataca gttgttccat acatataagt cccaccaact tctccccaat
    34981 aaatagactg tctctgtgta ataatcatct tttctccctt ccaaaaccag tctctaagac
    35041 cattctataa aagtgcaaaa accaagaaat attggtatct gtatcatcat tgtgtcgtcc
    35101 agcagttcct ttagataaaa tcttcgcacc tgtattacac agataagaca ctaattgttc
    35161 ataggcttgt ggatccattt cttcatcttt catataggat aaaccaaagg ttgtctttga
    35221 aaaatttgct tgtttaaaaa ttttccaaaa acgctgatcc aactccgtca tatcttgaga
    35281 acttgtccca cctgtatgat gaattaaaca atcaaaagct aagttagaga cacccggtgc
    35341 gtccaaacgt ccacgactag ctatagttcc taaattagcc aagggcttac caactatgat
    35401 ggcatggggt tcaaaagaag caccataata gagagccgga aacgttccca tagaaagacc
    35461 tgataaaatc aaatccttat gatcaaagcc taaataatca agatagtgag tgatcgtatc
    35521 ctttactttt ccctctagct catccgttcc gagataaaaa gctcccccct ctaaacgtgg
    35581 atcagaaaat aaaatgaagg gacatccaag agttttcatc ataaagtaac cctcgaatcc
    35641 ttctgcaggt cgataacctg caaaatagac agtcaaaggc ggtttaaaat cacctggatg
    35701 gaagaaatag tttatttcat cacgcttgga atcatgtagg atatttccac caagtacaaa
    35761 cttaccaaat tgttttcgac tccatcgttg gtgaagatta cctagtttga tagttccacg
    35821 acctcttgct tcaacagata tcgagagaaa gtagtctccg tctcgactat ccataaaaag
    35881 tgcttgctcc aagtcttttt cagatactat tacttcttca aagaaatcat caacagaccc
    35941 actccacatt ttacggatga ccaaacgaaa gtcacaattg ccttccttct catattcaag
    36001 ccacaactca atagggagtg ttttttgcac cataatatta taagcccaat aggcaagctg
    36061 ggcaaaatct tgcccaaact caccttccaa actgacataa tccaatcctt gataagaaat
    36121 agctcctgca aaatttggat tgacttgtat cgtcggagga aagagtttat caccataccc
    36181 accgccaaaa agagatttac ttaaatcatt gattagttgt tggggactta aaaaatcaat
    36241 tgcttgtgca catcgttttt ttagaaaatc cacaacctct ctatcattta tttgtagatt
    36301 ctggttatag aacaaagtat aaggttcaaa atattttaca taggaaaata agtctctgga
    36361 atactgacca tcttctatta aaactgcatg aaaacggtta atatcctctt tttcaattat
    36421 ttcacgaagg gcttttgatg agtttgggta aaaataatac caattcatat tttcaggtat
    36481 gtcatagtga ttttcccaat tggtaagccc tacctgtaaa atattaagtt cctttgacat
    36541 ttttaacctc ctctatccat ttctctaatt ttccaagaaa ttgttgtcct gtgtgctcct
    36601 taatcttatc aattgaatat atcaaggact cattccactc cttcaaccta tctgtgtaat
    36661 aatgtgcagc cttagaaaat tctgtaacat ctgctaacaa ataaccattt tttaaatgtt
    36721 caacatagac ggtttcaact aggttgattt gaggaattcc tgcactaatc ccagcaatct
    36781 gtgtgtagag atgaggatgt ctatttaaat ccacaatcaa acggacaaat tctagtgttt
    36841 ttatcaaatc taactcatca ttcaagttaa caaactgtaa gcgtaagtcc tggtgttgat
    36901 tttcttccag agggttttct gcatcaccat aatcaatcgc ctttcccaga ttttcggatt
    36961 gaatgttttc ttggatgaaa gactcaacaa tcccttcaac ctcattcatt tgctcctgac
    37021 tagcagcaaa ggctccaaaa atcacctcag tatccttatt ttcagcgaca aaggacaaga
    37081 cttgaagcag agcttgatta tcaatcccct gctcaaaatc cagttgaaaa taaatgatgg
    37141 attctttctt agtttggctt cgtcccaatc gtaatcgggt gtcaaaagat gataaatgat
    37201 aacattgatg cattcgttca ggatacaact cctgcaacaa tcgtaaacta tcctctctat
    37261 ccaccaaaat caaatccaat ttttcaaaag ttacatctaa atccctaaag gtatcttgag
    37321 gattacgtcc aatgaacaga ctcaacagtt taggattagt actaggtaaa cgatctagta
    37381 ccaactggtc atgatgagaa tgagaaggaa gcataaaaat atcctgatcc ttcacatacg
    37441 tttgcagata attctcaaaa aattctgcca ccagatctcc catattttga taggtaagca
    37501 ttttaaagcg ataacccaaa attggattga cttctacctg tcctccttct tttaaatgct
    37561 ctctaaattg ccaaactccc ttaggattca aatattcttg ataggctgct tgcccatctt
    37621 caaaaaaaat aatactcgat ataaaccctc gatgatccat gatatagtgc atatgtattt
    37681 gaccattcgg tccaaaatac tgtatatcac tgatgaaacc ttcaactccg tgttcaacct
    37741 tagcaaattt cttcccattt ttttgaacaa taatcgtaaa gggactataa ataaattcac
    37801 agtcatcatc ccactcaata tctctaatgc ttaaaacttg ggtatggaga ttatgaaaat
    37861 cttgcataac atcaaaaacg gaataagtat ccgtttctaa cacaccatgt ctatataaaa
    37921 aataacgtag atgaggctga taagctaata ctaatagacg agaatctata tcttgctctt
    37981 gaaagagccg gatctggtga aaggtatcat caaactctag acgaaaatga gaaaaatacc
    38041 atggagtgat atctgcatgc catgttcttt ctgacccata ccaagctgga ataaaataat
    38101 acataaaagt ctcctaaatc aaaggtttat aggtctcatt tagccttaaa gccttgacct
    38161 cgtctctaat cgtaaaaatc atgcctccaa acatcataaa caaacctgga atcattgcca
    38221 atcgtaataa cttttcatca aacaaaacaa aaagcatggg accacctgcc ataaccacat
    38281 taaagagacc acctatgact gagaaacgaa ggaccaatcg attaataaat cgactagtat
    38341 ccgcacctgg ataaatacca taaatgtatt ctccagattt tttcatacgg tctgcaatct
    38401 cttctccatt catcgtaaca aaagcaaaaa tgatactaaa taaaaataag accgaaatat
    38461 aaacatagac ccacaaaggc tttccaatca taaattcctt tgataaagcc gctaaccctg
    38521 aatgattagg gaaaataaat cccaacaaga tgaacaaata agctggtaca ctaagaaaac
    38581 tcatcacata catataaggc atcccacctg caggattcaa catgatttcg agataagaat
    38641 agcgtttaaa tcgattgtgt aagccaattt tattaacagg aaccaaatag cgagctcgat
    38701 acataagggc aagtaaataa gaaaagacaa gggttaataa agcaagtaac acaataatcc
    38761 ctgttggaat gtgtactgtc tgaaatgttt ccaaaacatc ctgaggaata tttaaaacca
    38821 tactggatag gagaatgact atagaacctc caatccccat actcgcattt aaatctgaca
    38881 accaaacaag aaaaaatgtt cctgctatca gcaatattgt attcattaga gcaaccaaaa
    38941 ttaccgagta ggaggattgt actggcaagc tcaagctaac tgccaaggat tgaatcacag
    39001 caattagcaa ggtcagatac atttttcgac gatcttgaat ttcaatcgct gtagaactta
    39061 gccccaattt tttagaatat gaaaacatct gccacaaaat catagctgac atccaagggg
    39121 ataatccaac agaaaaaatt gacaaacttc ttagatttcc acctgttagg gcggctgaga
    39181 aggctagata ggctgttgaa cctcctaaaa aatctttagt atttaggtca acaaaaggga
    39241 gaataatacg acttcccaat acatagataa acagtagaaa taaggtaaat agtccttttt
    39301 ttactgctat tgacgaataa atcttagtca taaaacactc ccctcacctt cattgtttgt
    39361 gatatggtaa ataacaattc ttttatttca acttttacca tttctttgtt ctgcatcaaa
    39421 aaacaacttt aatctgaaac aaatatcttt atattcctca gtatctgtta attgatggta
    39481 ttctaaagtt tgcttataat cttttaaaag attgacaaat cgaatacgca gagcggagac
    39541 atccaaacca gccaagacac aatccgatat tttcttagaa aacacttcaa caatatctct
    39601 tgcccaggaa agatcaaatt ccgttctcat gatgcttcct gaacgtcttc tatacagata
    39661 gagattatcg tttataaaga caattttaga ggctaaaaga taaaagcgat gcatagttgc
    39721 ttcatcatca aagcggcgac catttgaaaa gtgaacttca ttgaataatt cttttttaat
    39781 caacttaaat gtcggcaata taaaggccga gctattgaat ttccaatctc ctgcttgacg
    39841 gttcataatt tcttgtatag ctaattcttc gacgcaaaaa tcttgccctg ttacataaaa
    39901 ataaaaattc ccgtcacttt cgtcataatt atagtaattc cctataacaa catctgcatc
    39961 cttttcttgg acttgctgat aaagagtttc aatcatattt tcttttacga tatcatctga
    40021 atcaacaatc gtaatgtact ctcccttagc tacactaatc cccatatttt tagcttgtgc
    40081 tgcccccccc cttattttct tgatgcaata ctcgcacacg attatcttgc atcataaaag
    40141 catcacaaat ttccccagaa ttatccgtag aaccgtcatc aactaaaata atttctatat
    40201 tttgataggt ttgcttcaga atatgctcga cacactccac taaatatttt cccgtattgt
    40261 atatcggtac cacaacacta attaattcac tcattactct atctctatat tcttcattct
    40321 atccactaaa tcatctggtc tctccacaga aaaaataccg tcatagagtc catcatccat
    40381 acttttacgt gtgatatcaa aagcgaagat tttcttgcca ctttcttgtg cccttgtaac
    40441 tacattaaaa acctctccac ctgtattaat atctaaataa actatagaat cgtccaatag
    40501 ccaatcaatt ctactatgta atacattttg atatactgtt acattcatat actgtgatag
    40561 agaggtcagc tcctcagaac aatcacacgg tgcagccaaa tgaaaatgcc aatcaggtaa
    40621 ccgttgtact aaatactcta aatgttttat atctgcactc catgtcacaa gcatcacttg
    40681 ttttttagac tgattgcttc tttcaaaata atttaaatcg gaacgttgaa aagcaatctc
    40741 tgaccaatcc aaatctctat aaacccacca taattcacgt agtctagata tactatatgt
    40801 attccaaggt ttatcatgac tagcatagtg aacaattgta ggtggattgt catctagacg
    40861 ttcacattct tgagcaaggt gataaatatc aatacccacc atataattat atgttttatc
    40921 taaggctagc caattatcct caaaataaat atttaaaata ctctgatccc caagatgaac
    40981 aacttgattc tgctcggccg ctaattccaa taaactattg acaatagaat gttctttcca
    41041 ctttgcaaca tccattagta acataccagt attaaatcca gattttcgtc cttcataggc
    41101 atagacatca tcaacagcac caattgaata tcctttgaga tctatctcaa acaaagtagc
    41161 tagttcccca gttacaatga tatcggaatc cagatacaat accctatcag attcaaccac
    41221 ttctgtcgca aaaaatctaa agtaagaagc ataatttata tgaggtcctg ttttatagct
    41281 ttcaaaaagt tctttatcaa tgtgtacact gcgaattgta gaattcacaa cttccatttt
    41341 tctccccaat aatttaaacc attcaggagc tatatcactg ttgagaatat aaaaatcaac
    41401 atctctattg tgatacaata cagactttat agtcgtctct aaaggaataa gataggcatt
    41461 atctgccgct aatactattg attttctcat actgttctct acctcatgta agatctgatt
    41521 gctgcaacca tttttttcgg aatggagctt ggatagatac cttcatatga aatctcagaa
    41581 agattttcag agcaagtatt gtcgaaagct atcattggtt ttttgtactt aagcacaaac
    41641 tcatatgcat cttctaattt tcgatcatga ttcaaatcca aatacaaatc tgaagctatt
    41701 actatttgtt ccaataacat tggtaaggaa tttggatata cggtaacatt aggaaaacgc
    41761 gttaaagcta gtagttcatt tgccatattt gtataagcaa caatatgaaa atgaacttct
    41821 ggcaattgct ctgccaaata atcgattttc tcgacacacc aagaattcgt taaattaaca
    41881 cattgcaact taaatatttg tgatttacta ggatacttca cactctttga aaaccattca
    41941 tttaaaataa cagaccaatc catcaaagag tattcccacc aaacttctct tagacgtcca
    42001 acagaaaatt gattccaagg cttatcctga gaaatatagt gtaaaattag tggcagtggt
    42061 tcgagcggaa tatcaaaaat gaattgatgt ttaaaggctg ccgccccata atcatatcct
    42121 atttgaaaat tatattgatc ttcaagggaa ctatattgat ctttaaacaa catattcaaa
    42181 attgactggt ctccttcttc cacattctca tgttcttttt ctgttaagtc aatcaatttt
    42241 tgtcgaatag tttcagatcc ccattttttg ttgttaatca agagaacacc agcattgaag
    42301 ccgactcctg ctccaaagca agaacgagct gctgccaaat aattttcacc taagtctaat
    42361 tcaaacaaat cggtcaaatc accagtcaca atcaaatcac tatctagata gagaacttta
    42421 tcttctgtta caaaatctgg aataaaatag cgtgcaaatg tcatatgatt gatatgaggt
    42481 aatttattag accaattcat ttgaaactgt gagccaatta acttgcagtc aatcaagtcg
    42541 ccccccatct cttgtaaata tattcttatt tgactaaacc attcctgagg aatgtcctga
    42601 tttagcagat aaattttcaa atgactattg tgtctacaga gtgacttcat cgccgtttcg
    42661 atttgtcgaa tataagcgta atcacctgca aatactacag atttcttatc ttgtaacatt
    42721 ttatcgtctc tctatcttaa ttttcagtta tatttattac cattcaataa atagtctaac
    42781 atttctaaac gtcttgtttc cacattctcc ttaagttctc gttgaattgc aatatcatga
    42841 cgatatttca aatagaactt catctggtct aaatatttac tgacatcata tccttttagc
    42901 gctagcacgg caattcgttc taacctaaaa tcacaatcat cacgcatcat cttcggtgaa
    42961 taagaattag aaagtccttc tttcccaacc ctataccaat acaaaacttt attaatatag
    43021 attactttct caacctgcat aaataattta taattggttc cgatatcctc acacaaccga
    43081 ccaaatggga actggacttc ttgaaataat tcccttttaa atagaattcc ccaagaagtt
    43141 aaaaagctca tatcataccc ctctaataaa ggaagctctt tcattagaag ttttgaatta
    43201 taatttttca caaaataatc ggagtcaaat acgtgagtat agaataagcc atctgactca
    43261 ttataggttg aataagttga aacaacaatt tcagaatgct gctccttcaa agtttctata
    43321 agaattgata aatactcttc agtaacccaa tcatctgagt ctacaaatgt aatataatct
    43381 cctttagatc tctcgattcc aaaattacgt gcatccgcta ccccagcatt ttctttttcg
    43441 aaatagtgaa aacgactatc acgatcagca tactctcgac atattgctcc tgaattatct
    43501 gtagagccat catttactaa taaaacttca aaattttgat aaatctgttt cgataaactg
    43561 tctaagcagc tagaaataaa tggttctacg ttataaactg gtactataac acttattaaa
    43621 tctttcatac ttctcttttt gataaatcaa ctaaaatctg actgttatag gtttcatcat
    43681 caataaattc aatatcttgg tcgaaattat gttctgcagt atgtttatga gaaaatttaa
    43741 ccttagtttt agctcccatt ttcatccatt catactctga gtcaatatgg cctacatcca
    43801 aaacttgata gcccatctgg catagattat aactcagaac ttttgctgta ggacccagca
    43861 tacataaaat cagtcttcct tcagcatgtt tcataatttc ctcttgaatg ttatcgacaa
    43921 cggaataagc attatgagat ggacaaataa tccttttcac agatttgacc ttatcaaata
    43981 aatcatttcc aaccccagag cgagatgtaa tcccctctac aatcagaata tccctattct
    44041 cccaaatact ctttagcttt tcaaattgtc cttttgcctt actcttatct tcataatcaa
    44101 tgtatggacg cgagataaag gtagagccat accatgaacc cttacacaac tctttataaa
    44161 aatccatata aacattcata tgttctttcc agaatggtgt agcccaccaa gtaaagacaa
    44221 aacgatcgtc aaaagcatca ggtaaacaaa ctactaattt ctcgtcactc tcaagaccca
    44281 caatatgttt taattggtta gctaactcta gatcatgatt ttgataaggt ataggcaaac
    44341 cccacatcaa attgacttct ccgtctccaa aacgaactaa tgaggactta tttttgataa
    44401 cataatccag agtctgatca attccgataa ccttgattgg cttctctggt tctcccaaag
    44461 tctctccttt tatcaatgca tgaaatgctt gaagaagcct ttctggatgc tcacttgaat
    44521 ataaatgttg accatggttt ccattttgtg aagcataaaa tgccagcatt ggtttattca
    44581 atgatgatat ctccttgaca acctcactag tagaattgtg tagattaata tccaaataaa
    44641 catccatttg agatttgagt tcttcaaatt gatcgctttt cacttcagga tatagcttaa
    44701 catttttata tttttttgct agttcttgta acattggtcc cataactgtc caagctgcga
    44761 tatggaatgt taccatagga aaattcttaa caagataatc tatcttttgc aattcttgag
    44821 aatttgtaaa tgttaggcaa tggaaatcac taccttcata acaacttagg aatcttccat
    44881 ctcttctgta atctttaatc gcttgaacca tgactgaaac ttcatctttt ttgaatacta
    44941 gttggccagt attcccatgt tcagtcgtat caaatgatag aattggcttt tctaagatct
    45001 tcatatcctc taaaatagta tctaccacat gactgtagtt aatgtccaga tataaatcta
    45061 cacttctctc cagcttatct aatacaggag gaacaatttg tggatgcaat ctcacattat
    45121 tatatacagc taattttatc agcttatcac ccatgttcgt ccaagctgcg atatgaaata
    45181 ccacctcagg tagagctgta actagctctt ctattccttc taaatcttgg gaattcgtta
    45241 aggttaagca agaaaattcc ttatctagag gcgatattag ttcaaattct cccatatggt
    45301 gttgtaaaat ctgactccac tccaaatcat ggaattccca ccataaatca cgataacgat
    45361 tggctgtcaa ggtagtccag ggttttctgt aggtcgtaaa atgaatcacc actggtttat
    45421 cattaaaagc cagatgttcc tgccaattgt tatacaaagc cacaatatca tgccctactt
    45481 gtaaattata agctcgacct agctctaacc aatcatcttg taagacctga ttaaaaatcg
    45541 tttgatcacc attaaaatgc tcgaaacggc cttcttcaac ttccttcatt gtaacaatgc
    45601 tctgttcaat tagtcgttct tttaatttct cttgacgcca ttttttattg ttgattaata
    45661 gaacacctgt attaaatgta attccatctg tatctttaac tgctgcgagt gatttttctt
    45721 ctaaataaat actaaataat ttctctaaag aagtatttac aatcaaatca ctgtctaaat
    45781 ataaaacctt atcttcttgg atataatctg caataaaata tctagcatag gtaatgctac
    45841 taatgtgatc ttgcttttcc caatcttgaa acacagtttg ttcaggtagc ttcacatcga
    45901 taatctcact acctaacatg cgagctattt ttcgtggttt gcgaaaccaa tctggcataa
    45961 tatcttgatt taaaatataa atcttaacat ctcgattgtg gtatagaata gattttatgg
    46021 ttgtttctaa ctgcctggtg taattacgat caccagctaa tacaattgtt ttgttcattt
    46081 cactccctta atcaattccc aagtgataaa tcgcatctac caatagcttt ttagtgaaat
    46141 agccctcttt taacaaatag ctaaaggtct tgatacgatt cgtcatttct tgatattctt
    46201 gtagattcat tttatcaact atcgcatgta cttcctcaag gctatccgcc ataaaaccta
    46261 agccctgatc tactataaat ttagcagttg ataagttgct cggtacaatg actgggatgc
    46321 ccgctgttag ataggtacta accttatgag atatatttaa agtatagtat tgattgcttt
    46381 ctccgtcatt ttgatgcgtt ccccagacaa gaccaaagcc accttttgat aattctagca
    46441 acagttcctc atcttttttc catccttcaa tgctaagatt tctagcattg gaactagctt
    46501 ctcctttatt tgaaaacacc ctcaaaggcg tatcttgaga ccaattttgt aagtctggaa
    46561 aacgctctaa acttccagca aaaaaaagtt cttttttaaa agcaggggtg tataaggata
    46621 aatcatgagg atgatcccac atcccttgaa caagaatctt cttagtcgtc aatccttctt
    46681 ccatcaggcg tgttttcatt ctctctgacg gcactatcaa aacatctgat agattataca
    46741 tgtacatata atctttcatg agataatagt tactatcaaa catgagggga acaacatcat
    46801 ggataaagca aataattttc acctgcatat cctttagctt atcaaacaag agacgatcaa
    46861 attcaaagcc attccaggtt ggagactgaa aaactaggat atctccaata gagatactgg
    46921 ccataatgcc atccagacgc ttattcattt cagaaggact atctgaagaa atattgtaaa
    46981 aatagatgcc aacctctcta aatcccagtt gactggctat cttttgaaca gcattttgcg
    47041 ctaagataac cgtactatca ccagccatgc cgtataaatt tgttaaatgt agtttcatat
    47101 tatgctatct cattttttat tactatctac ggaagtaaag acaatttttc catcattctt
    47161 ctataaattt ctgtgaactg catattttgt tcttctaatt ttgctactac tctttgtaat
    47221 ctatttttat aaatcaaaat atgattggtc aagtcatagt ctgaacttgc taaaatagct
    47281 atcttttctt ccctagcccc aatttcatca tacatacgct tttcactcca tgtattcgat
    47341 aaagtatcag ataaaccaac acggtaccag taaacacaac gattcaaata gactatcctc
    47401 gaagctctta gaagtacctt ccatgtccag taagtatcct ctgcaatttt tcctatagga
    47461 aatggtaaat cttgtagtaa ctctctcttg aataacttca agacagctac agtccagttc
    47521 ccatttctga cttcttcgac accttcccta tccatgattg ctttaccttc tatcacttcc
    47581 agagaatcat ctggatccgt aacataagtc atatacacat agcgtgtttc atcataagaa
    47641 ttataacgcc cgatactaat atctgcgttt tcctttttca aagcaccata taatcggtct
    47701 aaagcatcat gttccaacca atcatcagag tctacaaaag taatgtacgc ccccccgaac
    47761 attcaatacc taggttacga gctgatgaaa gaccgccgtt tgctttctca aaatatttga
    47821 aacgagaatc tttctctaca aattcttcac atattttgga tgaatgatct ggagagccat
    47881 cattgattaa taaacactca aaattttgat acgtctgatt ctgaatgcta tccaaacaca
    47941 ttcgcaaata attctcaacg ttgtagattg ggactacaat actaattagt tcattttcca
    48001 tatactaact ttcctagact tgatttattc tatttccctc ttaatctctt ctctttacaa
    48061 aaagatttcc attattataa actacactgt ttgatggaat atcattgcgt atgagacaac
    48121 ctgctccaat aatggtatta tccccaatcg tcactccttt taaaatcgtg acattacttc
    48181 ctatccagca gtctctgcca actctaatcg gcgctgtagt ccactgccac ttttcaatct
    48241 tttcagcagt ataaatatgg tcgtgatcat aaaaacgaac accctctccc atcatggtac
    48301 cagctccgat ttcaattctt tccatacagt taaaactaca agagttgttg ataaaaacac
    48361 catcatgcag tattaactta ccagaagaga catgaaaatt ttcaaaattt ctacagatga
    48421 cattggctcc tacaatgaag tgagccttat catcgatttt aaagacattc caagtcccat
    48481 cttggatgat tttttcttga tgattagtgc ctactctgac ttcattcaga tagtctctca
    48541 ctctagaaac caatttgtct ggtgtacttg atagaaaaac ttcctgcccc tgatttccat
    48601 ggactgtatt gtcaaaagca aagacagatt ttccagcctg tacaaacttg gagacaatag
    48661 catctacttc ttgaaagtga ttgatatcta gtaaaaagtc acaggtatcg actagaaagt
    48721 ttagctgttc ttcatttgta atcgttcgga gacggacatt atagttcgtt tctaagctac
    48781 ggattttctc ggaaaattga acaggtgctg caatatgaaa acagatattt ggcaaggatt
    48841 caaggagata atccaactct tcaatctgat cactaacctt caaaatcaga gcatctccta
    48901 ctaaatgatt tttcttcatt tgcttatttc tctcaatttt tcaatcattg cttgaacttg
    48961 gtcaacagca tatgcctcct gacctacttc ataggtttta gtattttcaa aggataagat
    49021 aggcttgcca agattagcaa attgatcgag aatttcttct gtcttttcgc catgattaat
    49081 atctaaaagt acttgactgg tttctaccaa ttcattatcg acatctacca aatagtgaat
    49141 tccgttaaat atagtcacgt ttggataaat tgtcatctga gccaatcgat cactaactat
    49201 tactctagct gctatcttaa actgaatatc aggcaaggat tgaaccaatg tctcaatttg
    49261 ttcaatatgg tctgaggcag tatagattag acaagtgaaa ggttccttta ttggatagat
    49321 gtgagatctt tgtaatggat gtaaatgatg gttttgtccc aattctgtcc attcaagccc
    49381 atgatagtac caccaaactt cacgataggt ttgggccgcc aaatctttcc acggtttccg
    49441 atgagaaaga tagtgaataa tagcaggata atcctgaccc tcaggcaatt gataatcagc
    49501 aaactgttta tgaatgacaa tatgattata atcaaagtcc aattccaacc atttatgttc
    49561 aaaaagcata ttcaagatgc tctgatctgc ctgatccacc ttatcatgcc attcattggt
    49621 tacatcaatt aatttttggg tcatattctc ttttttccaa aaagcattgt ttaccaagag
    49681 aacaccggca ttaaagattt cttgaccaaa ataagctctg cccccaaaat ctctaacagc
    49741 agccaaagga taatcttgta agtctgtagc aaacaagtca tccagatttt tcgttacaac
    49801 tagatcacag tccaagtaga gggccttgtc ttcttgcacg aaatcagcta tgaaatagcg
    49861 taaaaagact gtgtaactaa tatccgattt ataacatgaa atttgctcag aagttacccg
    49921 acaattaata atttctgagt caaacttctc taagcgctta tttaattgct taatccattc
    49981 atttggaaaa tcgctatgaa tcagataaaa acgaatcgaa cgattatgat aacaaataga
    50041 cttgattgtc gttaaaactt ggtctacata gccatagttt gctgcgagga caatggcttt
    50101 cttttcactt tcctcttgtc tcgatagctg atttaaaagc ctttgtttca tttcaaactc
    50161 tttatacgtt gctgtgtcag ataaaccact agcttgaccg ttggcgagac tgacttccaa
    50221 catctgacga taaactgcca agtgtttctc tagaggataa cccatattag ctagtagcgt
    50281 aatacgttca gacatagcat caactaaagc gtgcatccac ttttctgtcc aaactcttga
    50341 taaactacct tttctaatcc gataagcata aagactttta tttaaataaa ttaccttttc
    50401 tgataataaa tatacctttt gattgaggta accatcttct cctaatttac ctatgtcaaa
    50461 gcgcaactgc tcaaacaatc ttgccttata gagtttaccc caagcagata tcaaagcaaa
    50521 actcttcatt tcttgagttt catacaagtt ctcaaagata gaaacattat catatacttt
    50581 ctcataatag gagtctccca atatatgaaa gtagaacatt ccttcacttt cgttgaaaga
    50641 ataataatta ccaactgcaa tatcagcctg atactctact atttttttat atagagtttc
    50701 tacataatct tgctcaatcc aatcatccga gtccacaaag gtcacataat ttctggacat
    50761 attattcaga ccggtgtttc gtgcggcaga aagaccagca ttttcttgtt ctatatagag
    50821 aattcggtga tccatttctg aaaattcttt acaaatttca cctgaagcat ccgtagaacc
    50881 atcattaacg acaacaatct caatattttt atatgtttga gtaataatac tatctaggca
    50941 cttcctcaga tagttttcca cattgtatac tggtacaatg actgttattt tatcatccac
    51001 tagcaatttt ctccttgatt tatgtgcgcg agttagttta gcctaacttt acaggttgtc
    51061 ttactcttct tcatcacgtt tacggcgttt cgcaaccaat ccaatacctg taacagctgc
    51121 tagaactcca agtaacacag atccaattga cgactcagta cctgtattag gcaattcttt
    51181 ttgggattta cctgtcgatc cagaagtatt tccaacttgt gagttcgaat ggtttgctga
    51241 atttgaaact gatgtacttg ctgaagctga cgcacttgtt gatgctgaag ctgaagcact
    51301 ggtgcttgct gaggccgagg cactggtgct tgctgaagcc gacgcactcg ttgatgctga
    51361 agctgacgca cttgttgagg ccgaagctga agcacttgtt gatgctgagg ctgacgcact
    51421 ggtacttgct gaagctgagg cactggtgct tgctgaggct gaggcgctgg tacttgctga
    51481 ggctgacgca ctggtgcttg ctgaagctga ggcgct
//
LOCUS       DAVKGC010000004        40260 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKGC010000004 DAVKGC010000000
VERSION     DAVKGC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40260
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.004"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            5..75
                     /locus_tag="VW049_000164"
     tRNA            5..75
                     /locus_tag="VW049_000164"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VW049_000165"
     tRNA            110..183
                     /locus_tag="VW049_000165"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VW049_000166"
     tRNA            266..355
                     /locus_tag="VW049_000166"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            366..439
                     /locus_tag="VW049_000167"
     tRNA            366..439
                     /locus_tag="VW049_000167"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Met,seq:cat)
     gene            443..515
                     /locus_tag="VW049_000168"
     tRNA            443..515
                     /locus_tag="VW049_000168"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Phe,seq:gaa)
     gene            528..608
                     /locus_tag="VW049_000169"
     tRNA            528..608
                     /locus_tag="VW049_000169"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Tyr,seq:gta)
     gene            613..683
                     /locus_tag="VW049_000170"
     tRNA            613..683
                     /locus_tag="VW049_000170"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Trp,seq:cca)
     gene            698..770
                     /locus_tag="VW049_000171"
     tRNA            698..770
                     /locus_tag="VW049_000171"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:His,seq:gtg)
     gene            781..852
                     /locus_tag="VW049_000172"
     tRNA            781..852
                     /locus_tag="VW049_000172"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:813..815,aa:Gln,seq:ttg)
     gene            862..945
                     /locus_tag="VW049_000173"
     tRNA            862..945
                     /locus_tag="VW049_000173"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:896..898,aa:Leu,seq:caa)
     gene            1052..2329
                     /locus_tag="VW049_000174"
     CDS             1052..2329
                     /locus_tag="VW049_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEU9431364.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDETDFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2405..3889
                     /locus_tag="VW049_000175"
     CDS             2405..3889
                     /locus_tag="VW049_000175"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9431365.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNTQGQYELTDFDVEILELFAAEYATEEETAAEIKRVCESYS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTTVAAADMQAAVEAYLGL"
     gene            3950..5230
                     /locus_tag="VW049_000176"
     CDS             3950..5230
                     /locus_tag="VW049_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9431366.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMILKG"
     gene            5234..5857
                     /locus_tag="VW049_000177"
     CDS             5234..5857
                     /locus_tag="VW049_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9431367.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDKNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFEAER"
     gene            6019..7161
                     /locus_tag="VW049_000178"
     CDS             6019..7161
                     /locus_tag="VW049_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9431368.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETTRPVEEATPQETTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQSEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDRIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7570..7908
                     /locus_tag="VW049_000179"
     CDS             7570..7908
                     /locus_tag="VW049_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9431369.1"
                     /translation="MTRYQILLFLLEHSLCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            7919..8287
                     /locus_tag="VW049_000180"
     CDS             7919..8287
                     /locus_tag="VW049_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9431370.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYNALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            8394..9012
                     /locus_tag="VW049_000181"
                     /pseudo
     CDS             8394..9012
                     /locus_tag="VW049_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(9039..9896)
                     /locus_tag="VW049_000182"
     CDS             complement(9039..9896)
                     /locus_tag="VW049_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9431371.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10026..11168
                     /gene="tgt"
                     /locus_tag="VW049_000183"
     CDS             10026..11168
                     /gene="tgt"
                     /locus_tag="VW049_000183"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9431372.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNIIEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11266..13083
                     /locus_tag="VW049_000184"
     CDS             11266..13083
                     /locus_tag="VW049_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9431373.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPSYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHRMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13236..14387
                     /gene="nagA"
                     /locus_tag="VW049_000185"
     CDS             13236..14387
                     /gene="nagA"
                     /locus_tag="VW049_000185"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9431374.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14550..15608
                     /locus_tag="VW049_000186"
     CDS             14550..15608
                     /locus_tag="VW049_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9431375.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15759..16124
                     /locus_tag="VW049_000187"
     CDS             15759..16124
                     /locus_tag="VW049_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9431376.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16200..17141
                     /locus_tag="VW049_000188"
     CDS             16200..17141
                     /locus_tag="VW049_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9431377.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17089..18105
                     /locus_tag="VW049_000189"
     CDS             17089..18105
                     /locus_tag="VW049_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9431378.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18107..18433
                     /gene="comGC"
                     /locus_tag="VW049_000190"
     CDS             18107..18433
                     /gene="comGC"
                     /locus_tag="VW049_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU9431379.1"
                     /translation="MKKMMTFLKKAKVKAFTLIEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLQADGRITEEQAKAYKEYHDKNGVA
                     NRKVND"
     gene            18426..18830
                     /locus_tag="VW049_000191"
     CDS             18426..18830
                     /locus_tag="VW049_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9431380.1"
                     /translation="MIKAFTMLESLLALSLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTNLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18835..19095
                     /locus_tag="VW049_000192"
     CDS             18835..19095
                     /locus_tag="VW049_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431381.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19058..19519
                     /locus_tag="VW049_000193"
     CDS             19058..19519
                     /locus_tag="VW049_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9431382.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVESNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19497..19910
                     /locus_tag="VW049_000194"
     CDS             19497..19910
                     /locus_tag="VW049_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9431383.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            20047..20634
                     /locus_tag="VW049_000195"
                     /pseudo
     CDS             20047..20634
                     /locus_tag="VW049_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            20695..21648
                     /locus_tag="VW049_000196"
     CDS             20695..21648
                     /locus_tag="VW049_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9431384.1"
                     /translation="MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFTEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNKIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21699..22889
                     /locus_tag="VW049_000197"
     CDS             21699..22889
                     /locus_tag="VW049_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9431385.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23037..23408
                     /gene="rnpA"
                     /locus_tag="VW049_000198"
     CDS             23037..23408
                     /gene="rnpA"
                     /locus_tag="VW049_000198"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9431386.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYQEGNGSEKETKVD"
     gene            23383..24207
                     /locus_tag="VW049_000199"
     CDS             23383..24207
                     /locus_tag="VW049_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9431387.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNEVTSDITADSADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24226..25212
                     /locus_tag="VW049_000200"
     CDS             24226..25212
                     /locus_tag="VW049_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9431388.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25263..25886
                     /locus_tag="VW049_000201"
     CDS             25263..25886
                     /locus_tag="VW049_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9431389.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKVFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26017..27474
                     /locus_tag="VW049_000202"
     CDS             26017..27474
                     /locus_tag="VW049_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9431390.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27497..27778
                     /locus_tag="VW049_000203"
     CDS             27497..27778
                     /locus_tag="VW049_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9431391.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27855..28340
                     /locus_tag="VW049_000204"
     CDS             27855..28340
                     /locus_tag="VW049_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9431392.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28357..29022
                     /locus_tag="VW049_000205"
     CDS             28357..29022
                     /locus_tag="VW049_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9431393.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVSQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIVGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            29026..29889
                     /locus_tag="VW049_000206"
     CDS             29026..29889
                     /locus_tag="VW049_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9431394.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     GRLDWSKEERLEVVKAIYGTGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29891..30595
                     /locus_tag="VW049_000207"
     CDS             29891..30595
                     /locus_tag="VW049_000207"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9431395.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVEPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30772..32445
                     /locus_tag="VW049_000208"
     CDS             30772..32445
                     /locus_tag="VW049_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9431396.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTLSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFLNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32557..33648
                     /gene="ulaG"
                     /locus_tag="VW049_000209"
     CDS             32557..33648
                     /gene="ulaG"
                     /locus_tag="VW049_000209"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9431397.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33762..35738
                     /gene="tkt"
                     /locus_tag="VW049_000210"
     CDS             33762..35738
                     /gene="tkt"
                     /locus_tag="VW049_000210"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9431398.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35855..36154
                     /gene="yajC"
                     /locus_tag="VW049_000211"
     CDS             35855..36154
                     /gene="yajC"
                     /locus_tag="VW049_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9431399.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36200..36628
                     /locus_tag="VW049_000212"
     CDS             36200..36628
                     /locus_tag="VW049_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9431400.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36630..37040
                     /locus_tag="VW049_000213"
     CDS             36630..37040
                     /locus_tag="VW049_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431401.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37340..39991
                     /gene="adhE"
                     /locus_tag="VW049_000214"
                     /gene_synonym="adhC"
     CDS             37340..39991
                     /gene="adhE"
                     /locus_tag="VW049_000214"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9431402.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12197 a         7220 c         9064 g        11779 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attaacgcgg gatggagcag ctcggtagct cgtcgggctc ataacccgaa ggtcgtaggt
      421 tcaaatcctg ctcccgcaat aaggctcggt agctcagttg gtagagcaat ggattgaagc
      481 tccatgtgtc ggcggttcga ttccgtctcg cgccatttat atattttgga agggtagcga
      541 agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt cgaatccctc
      601 cccttccatt ttacgggcat agtttaaagg tagaactaag gtctccaaaa ccttcagtgt
      661 gggttcaatt cctactgccc gtgttaatag aattatggcg ggtgtggtga agtggttaac
      721 acaccagatt gtggctctgg catgcgtggg ttcgatcccc atcactcgcc tattttatat
      781 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
      841 cagctacccc agttactatt tgccggcgtg gcggaattgg cagacgcgct ggactcaaaa
      901 tccagtgtcc gcaaggacgt gccggttcga ccccggccgc cggtatagta tagtgttagg
      961 aacgttgtta ttcttcgttc cttttttata ttatttttgg tataattata gttattcaaa
     1021 ttttatttag attaagaaag tgtaggggag tatgtcttgt tctatcgatt tattaaaaca
     1081 tcggtatttg aaaaatatta aagaaaatcc tgaattgttt gtcggaattg agttggagta
     1141 tcctgttgca agtttagaag gggatgctac agatgttgaa gttatgaagg atctatttca
     1201 ttatttagtt tctactttgg atctcaccgt agcaaaggta gatgattttg gcaatctgat
     1261 ccagttagta gatccgataa gtcaggatgc tattttattt gaagtttcct atacaacgat
     1321 tgagtttgca tttggtaagg ctgaaacgat tcaagaggtc gaaaatcgtt tcaataatta
     1381 tatgaatgta attcagagaa agttagctga atcaaatcat gctattgttg gctgtggtat
     1441 ccatcccaac tgggataaaa atgagaattg tccagtggct tatccacgct atcagatgtt
     1501 gatggattat ttgaatttga gtagaaatat tattaaatca gatttacatc atttccctga
     1561 atatggtact tttatctgtg ggagccaggt tcagctggat atttcaaaaa ccaactactt
     1621 acgggtgatt aatgctttta ctcaaattga agcggctaag gcttatttat ttgcaaactc
     1681 tgaattttcg ggtgcggatt gggatacgaa aatttcaagg gatattttct gggaagaatc
     1741 tatgcatggt atctatccag agaatgttgg ggtcaatgct agactcctta atgatgaaac
     1801 tgattttttt gactatctaa atcattctgc gatttttact gcggaacgtg atgggcagac
     1861 ctattatttt tatcctattc aggctgggga ctatttggct acgtccgaaa tccaagcatt
     1921 tgctctgaat ggggatgagg ttattattta cccccaagag aaggattttg aaactcatcg
     1981 tagttaccag taccaagatt taacgactcg aggaacagtt gagtttcgta gtgtgtgtac
     2041 acagccactt gataggactt ttgcttctgc agcttttcac ttgggattat tggttaattt
     2101 agacaagtta gaagcttact tagaaacagc acctttcttt aaagtatttg gttatgatta
     2161 caagtcttta aggagacaat tttctaagaa aaatcttaca gatgaggaag aaactacgat
     2221 tattgaattt tccaaagact tactcctact agctgaggag ggactagtgg tgagaaataa
     2281 ggaagaaatg acctatttac agcctttgag agaagaattg agcctataat ttctcttata
     2341 aagggagaat tttctgaaaa atcatgatat aatggacgag actatagata aaggatagag
     2401 agtaatgaca ttagtttatc aatcaacgcg tgatgccaac aatacagtaa ctgccagcca
     2461 agcaattttg caaggtttgg cgacggacgg cggtttgttt acaccggata cttatccaaa
     2521 ggtagatttg aactttgaca aattgaaaga tgcttcttac caggaagttg ctaagctagt
     2581 tttgtcagca tttttagatg actttacagt tgaggagttg gactactgta tcaacaatgc
     2641 ctacgatagc aaatttgata ctccagctat tgcaccatta gtgaaattag atgggcaata
     2701 caatttggaa cttttccatg gttcaacgat tgcctttaag gatatggcct tgtctatttt
     2761 gccatacttt atgacgactg ctgctaagaa acatggtttg gagaacaaga ttgttatctt
     2821 gacagcgaca tctggtgaca cggggaaagc tgctatggcg gggtttgcga atgtgcctgg
     2881 tactgagatt atcgtctttt atccaaagga tggtgtcagc aagattcaag agttacaaat
     2941 gaccactcag actggcgaca atactcatgt tattgctatt gatggtaact ttgacgatgc
     3001 gcaaacaaat gtgaagcaca tgtttaacga cgtggctctt cgtgaaaaat tgactaccaa
     3061 caagttgcaa ttttcatcag ctaactctat gaacattggt cgtctggtgc cacaaattgt
     3121 ttattatgtt tatgcttacg ctcaattggt taagactggt gaaattgtag ctggtgaaaa
     3181 ggttaacttc acagtaccaa caggaaactt tggaaatatc ttggctgcct tttatgccaa
     3241 acaaattggt ttgccagttg gtaaattaat ctgtgcttca aatgacaaca atgttttgac
     3301 agacttcttt aaaacacgtg tctatgacaa aaaacgtgag tttaaggtaa caaccagccc
     3361 atctatggat atcttggtat cttcaaactt ggagcgcttg attttccatc ttttggggaa
     3421 tgatgcggtt aagacggctg aactcatgaa tgccttgaat acacaaggac aatatgaact
     3481 gactgatttt gatgtagaga ttctggagct ctttgcagct gaatatgcga ctgaggaaga
     3541 aacggcagca gagatcaagc gtgtttgtga gtcatattct tatatcgagg accctcatac
     3601 agctgttgct tcagcagttt ataaaaaata ccaatcggcc actggagatg taactaagac
     3661 agtgattgct tcaacagcta gtccatacaa gttcccagta gttgcagtag aagctgtaac
     3721 tggaaaagca ggtttaacag actttgaagc cttggctcaa ttacatgaaa tctcaggcgt
     3781 tgcagtgcca ccagcagttg atgggcttga gacagctcct gttcgtcaca agacaactgt
     3841 agcggctgct gatatgcaag cagcggttga ggcttattta ggactttaag atagagggag
     3901 taaactcggt tgggaaacca actgagtttc ttttcatcag gaggagagat tgtttaagaa
     3961 aaataaagac attcttaata ttgcattgcc agctatgggt gaaaactttt tgcagatgct
     4021 aatgggaatg gtggacagtt atttggttgc tcatttagga ttgatagcta tttcaggggt
     4081 ttcagtagct ggtaatatta tcaccattta tcaggcgatt ttcatcgctc tgggagctgc
     4141 tatttccagt gttatttcaa aaagcatagg gcagaaagac cagtcgaagt tggcctatca
     4201 tgtgactgag gcgttgaaga ttaccttact attaagtttc cttttaggat ttttgtccat
     4261 cttcgctggg aaagagatga taggactttt ggggacggag agggatgtag ctgagagtgg
     4321 tggactgtat ctatctttgg taggcggatc gattgttctc ttaggtttaa tgactagtct
     4381 gggagccttg attcgtgcaa cgcataatcc acgtctgcct ctctatgtta gttttttatc
     4441 caatgccttg aatattcttt tttcaagtct agctattttt gttctggata tggggatagc
     4501 tggtgttgct tgggggacaa ttctgtctcg tttggttggt cttgtgattt tgtggtcaca
     4561 attaaaactg ccttatggga agccaacttt tggtttagat aaggaactgt tgaccttggc
     4621 tttaccagca gctggagagc gacttatgat gagggctgga gatgtagtga tcattgcctt
     4681 ggtcgtttct tttgggacgg aggcagttgc tgggaatgca atcggagaag tcttgaccca
     4741 gtttaactat atgcctgcct ttggcgtcgc tacggcaacg gtcatgctgt tggcccgagc
     4801 agttggagag gatgattgga aaagagttgc tagtttgagt aaacaaacct tttggctttc
     4861 tctgttcctc atgttgcccc tatcctttag tatatatgtc ttgggtgtac cattaactca
     4921 tctctatacg actgattctc tagcggtgga ggctagtgtt ctagtgacac tgttttcact
     4981 acttggaacc cctatgacga caggaacagt catctatacg gcagtctggc agggattagg
     5041 aaatgcacgc ctcccttttt atgcgacaag tataggcatg tggtgtatcc gcattggaac
     5101 aggatatctg atggggattg tgcttggttg gggcttacct ggtatttggg cagggtctct
     5161 cttggataat ggttttcgct ggttatttct acgctatcgt taccagcgct atatgatctt
     5221 gaaaggatag gaaatgcaaa aaacagcttt tatttgggat ttagacggga ctttattgga
     5281 ctcttacgaa gcgattttat cagggattga ggagactttt gctcagtttt ctattcctta
     5341 tgataaggag aaggtgagag agtttatctt caagtattcg gtgcaagatt tgcttgtgcg
     5401 ggtggcagaa gataaaaatc tggatgttga ggtgctaaat caggtgcgtg cccagagtct
     5461 ggctgagaag aatgctcagg tagttttgat gccaggtgcg cgtgaggtgc tagcttgggc
     5521 agacgaatca ggaattcagc agtttatata tactcataag gggaacaacg cttttaccat
     5581 tctcaaggac ttgggggtgg aatcctattt tacagagatt ttaaccagtc agagtggctt
     5641 tgtgcggaag ccaagtccag aagcggctac ctatctgcta gataagtatc agttgaattc
     5701 tgataatact tattatatag gggatcggac tctggatgtg gaatttgccc agaatagtgg
     5761 gattcaaagt atcaactttt tagagtctac ttatgaaggg aatcacagga ttcaagcgtt
     5821 agcagatatt tcccgtattt ttgaggctga acgataaaaa gattgtgtca gttttgtgac
     5881 agagacctaa caaactattt caagtaacct agtttgttac aaggaataga cagttctgtt
     5941 aaataggccc gagagggctt tttttctaca ttttttgtgt tatcatagac aggtactcat
     6001 ttgaaaggat ttgaaagaat gaagaaaaga atgttattag cgtcaacagt agccttgtca
     6061 tttgccccag tattggcaac tcaagcagaa gaagttcttt ggactgcacg tagtgttgag
     6121 caaatccaaa acgatttgac taaaacggac aacaaaacaa gttataccgt acagtatggt
     6181 gatactttga gcaccattgc agaagccttg ggtgtagatg tcacagtgct tgcgaatctg
     6241 aacaaaatca ctaatatgga cttgattttc ccagaaactg ttttgacaac gactgtcaat
     6301 gaagcagaag aagtaacaga agttgaaatc caaacacctc aagcagactc tagtgaagaa
     6361 gtgacaactg cgacagcaga tttgaccact aatcaagtga ccgttgatga tcaaactgtt
     6421 caggttgcag acctttctca accaattgca gaagctccaa aagaagtagc atcaagttca
     6481 gaagttacaa agacagtgat tgcttctgaa gaagtggcac catctacggg cacttctgtc
     6541 ccagaggagc aaacggccga aacaactcgc ccagttgaag aagcaactcc tcaggaaacg
     6601 actccagctg agaagcagga aacacaagca agccctcaag ctgcatcagc agtggaagca
     6661 actacaacaa gttcagaagc aaaagaagta gcatcatcaa atggagctac agcagcagtt
     6721 tctacttatc aatcagaaga aacgaaagta atttcaacaa cttacgaggc tccagctgcg
     6781 cccgattatg ctggacttgc agtagcaaaa tctgaaaatg caggtcttca accacaaaca
     6841 gctgccttta aagaagaaat tgctaacttg tttggcatta catcctttag tggttatcgt
     6901 ccaggagaca gtggagatca cggaaaaggt ttggctatcg actttatggt accagaacgt
     6961 tcagaattag gggataggat tgcggaatat gctattcaaa acatggccag ccgtggcatt
     7021 agttacatca tctggaaaca acgtttctat gctccattcg atagcaaata tgggccagct
     7081 aacacttgga acccaatgcc agaccgtggt agtgtgacag aaaatcacta tgatcacgtt
     7141 cacgtttcaa tgaatggata aacccgactt gataacatca ttttgacgaa tgagatctag
     7201 ctttcgtgat ggaaagcgat tctcgttcgt tttttctttg tcatactctt cgaaaatctc
     7261 ttcaaaccac gtcagtttta tctgaaactt caaagctgtg ctttgagcaa cctgcgacta
     7321 gcttcctagt ttgctttttg attttcattg agtatcaatt tgaatggaaa atggaaagtt
     7381 atcatcttgt aatgagttaa gcaacattct tgcaatctat tttactttat atcacaatta
     7441 attagtcaaa tattgataaa tcaataaaaa gagaggggaa gaaatgctag agattcaaga
     7501 tttactgtat caactccgct tgtctgagca agcgagtacg caattgtttg aaaaaggctt
     7561 gggattagtt tgacacggta tcagatttta ctgtttttgc tggagcattc tctttgtaac
     7621 caaatggcgg ttcaggagcg tttgaaaatt gatcaggctg ctttgacacg gcatttcaag
     7681 attttggaaa cggaaggttt ggtggagcgt catcgtaatc ctgaaaatca gcgggaagtg
     7741 ttggtagagg ctgcgaagta tgccaaggag cagttagtgg tgaatccccc tctgcaacat
     7801 atcaaggtta aggaagagat ggaaagtatc ttaacagagt ttgagagaac agaactcagc
     7861 cgtttattaa ataaattggt tttgggtatt gaaaatatag aaatttaagg agaaatagat
     7921 gtcaattatt acaattattt taacaacgat cgttgctttg gagcattttt acatttttta
     7981 tttggaaagt attgccacgc aatcagatgc gactagtcgt gtatttaata tggaaaagga
     8041 agaattggct catccgtcag taagttcatt gttcaaaaat caagggattt ataacgctct
     8101 gctaggagtc tttctcttgt atgtcattta tttctcacag aatttagaaa ttgtgactat
     8161 ttttgtctta tttgtgattg gtgctgcgac ttacggctct ttaacagcgg ataaaaaaat
     8221 tattttgaaa caaggtggat cagctatttt ggccttgatt agtattttac tctttaaata
     8281 cacttgaagg tcgattctaa tctcgctaat cctttttaat ccagaataag ggaaatatgt
     8341 tatacttgtt tttaagaaaa aagtttcatt gaattggttt tgaggagtta gaaatgaaag
     8401 tattagtgac aggttttgag cccttttgag gccattaaag gtttaccagc tgaaatccat
     8461 ggtgctgagg tccgttggct agaggtgccg acagtttttc acaaatctgc tcaagtattg
     8521 gaagaagaga tgaatcgtta tcaacctgac tttgtccttt gtattgggta agctggtgga
     8581 agaactagtt tgacacctga acgagtggcc attaatcaag acgatgcacg tacttctgat
     8641 aacgaagata atcaaccgat tgaccgtccc attcgcccag atggtgcttc ggcctacttt
     8701 agtagtttgc cgattaaagc gatggttcaa gctataaaaa aagagggctt accggcctct
     8761 gtttccaata cggcagggac ttttgtctgc agccatttga tgtatcaggc tctctatttg
     8821 gtagaaaaga aatttccata tgttaaggca ggttttatgc atattcctta tatgatggaa
     8881 caggtggtga acagaccgac tactccagct atgagtttag tggatattcg gcgagggata
     8941 gaagcagcaa tcggcgctat gatagaacat ggagatcagg acctcaagtt ggtaggcgga
     9001 gaaactcatt gatagaaaaa agcttgaggg aaaaaccttc aagcttttgg acgttttcga
     9061 gccaatactg ctcggtaaaa cataatttta gtgcattgga tataaggtag gagtgaaaaa
     9121 ctagcaatgc caaaggtaat ccaattgagg aagtaccaag gaagaagctg taaatctagg
     9181 acaaagtgct ggaacttgta gcccttcata aaggaacgac tagtttttag gattcgtctt
     9241 ggtgggacct gtcctaggtc tagactataa cagagaagaa attccacctg tgaataggca
     9301 taatactgtg gaatatagag gatatttcct acaatgatca agatgagact tgcaagaaag
     9361 tagagtccaa agaccatgag gaaacgctcg gtttcaactg atgagagatc tagatttgga
     9421 aactcaggat gtagggtgac gaattttttg gctaaaaagc tactataaaa gaggaggtaa
     9481 atcccaagta aattagggat actccataaa aagagataga aacgtttgag aagtagggtc
     9541 aaaaaggttt gagaaaagcg ctcctcatca aagagagcta ggctgttttt tacagatggc
     9601 tccgttttag aatctttcat gagtgtcagt gttgcataga cggaactggt caaaagaata
     9661 gtcccgataa aggagactag tagaggaaag aggtaggttt gaagtatttg gccaagtatg
     9721 ctgaaaaatg gctgttctaa aacagtcccg tggatccgag ataagggatt aagaaaacta
     9781 gataagatga ccagcatact gggaaggata tagaggagaa agagacgggg ggtgtcagcc
     9841 tgaaaatgtt ttgactcctg acgaattgtt tttaaatcaa tttttggata gttcattctc
     9901 ttattatacc atagttctta tacatagttc gtgacagttc ctactttttt tgataaaatc
     9961 atacagtgtg tccttgggca cactgtatga actgggactg tctttcccag cttcggaggt
    10021 aaaaaatgtc agattcacca atcaaatatc gtttgattaa gaaagaaaaa cacacaggag
    10081 ctcgtctggg agaaatcatc actccccacg gtacctttcc gacacctatg tttatgccag
    10141 ttgggacaca agccactgtc aaaactcagt cacctgaaga attgaaggag atgggttcgg
    10201 gaattatcct atcaaacacc tatcatctct ggcttcgccc tggagatgaa ctcattgcac
    10261 gcgctggtgg tctccacaag ttcatgaatt gggaccagcc tatcttgaca gatagtggtg
    10321 gttttcaggt ttattcttta gcagatagcc gtaatatcat agaagaagga gtaaccttta
    10381 aaaatcatct aaatggttct aagatgttcc tatccccaga aaaagccatc tctattcaga
    10441 ataatctggg ttcagacatc atgatgtcct ttgatgaatg tcctcagttt tatcaacctt
    10501 atgactacgt taagaaatcg atcgagcgta ccagccgttg ggctgagcgt ggtttgaagg
    10561 ctcaccgtcg tccacatgac caaggtttgt ttggaattgt gcaaggtgca ggatttgaag
    10621 accttcgccg ccaatcagct catgatcttg tcagcatgga tttctcaggc tactctatcg
    10681 gtggtttggc agtgggagaa acccatgaag agatgaatgc ggtcttggac tttacaactc
    10741 aactgctgcc tgaaaataaa cctcgttatc tgatgggtgt gggagcgcca gatagcttga
    10801 tcgatggggt cattcgtggg gtggatatgt ttgactgtgt cttaccgact cgaattgctc
    10861 gtaacgggac ttgtatgacc agtcaaggac gtttggttgt gaaaaatgcc cagtttgctg
    10921 aggactttac gccactggat cctgagtgtg attgctacac atgtaataac tatacacgcg
    10981 cttaccttcg tcacctgctc aaggctgatg aaacctttgg tatccgcttg actagctacc
    11041 acaatcttta cttcttgctt aacctgatga agcaagtgcg acaagccatc atggatgaca
    11101 atctcttgga attccgtgag tattttgtgg aaaaatatgg ctataataag tcaggacgta
    11161 atttctaaaa tggaattgat ataaaaaaat cctaagtttt ctcttaggat ttttcttctt
    11221 tttttgatag aataaagtgt acaatgaaag gaagaataaa ctcgtatgcg cattaaatgg
    11281 ttttccttga ttaggattat aggtttactt ttggtactct tgtatcactt ctttcagacg
    11341 atctttcctg gaggattttt cggggtagat gtctttttca cattttcagg tttcctgatt
    11401 acagctctac tcattgaaga attttctaag aacaatgaga ttgatttgat aggatttttt
    11461 agaagacgct tttatcggat tgtgccacct gtggttttga tggtcttggt gaccatgcct
    11521 tttactttct tggttcgcca agactatgtt gctggaattg gtggccagat tgcgggcgtc
    11581 ttaggcttta tgaccaactt ctatgaactc ctaacaggtg ggagttatga atctcagttc
    11641 attcctcatt tgtttgttca taattggagt ttggcagttg aggttcacta ctatattctt
    11701 tggggattgg cagtttggtt cttatccaaa caagctaaat caaatggtca gttgaagggg
    11761 atggtctttc tcttatctgc tgctgccttc ttgatcagtt tcttctccat gtttattggt
    11821 agttttctag tgacctctta ttcctctgtt tatttctcca gtttaactca tgtctatcca
    11881 ttctttttgg gaagtatgtt agcaactatt gtaggcgttc gtcagacgac ttccctcgtc
    11941 aagcagttgg ataaaatctg ggatttacga aagactttgg tagtttttgg aggaggcttt
    12001 ggtttcctag ttcttttgac tttctttgtc aaattcactt atctttttgc ctatcttatc
    12061 ggcttcttgc ttgccagtct tgcagctctt gccatgattc tggcggcgcg tgtcttacat
    12121 gaaaagacac atcatataca ggagccgaag attatcagct ttttagcgga tactagctat
    12181 gcggtttatc ttttccattg gccttcctat atcattttct cacagttgac atcaaatctt
    12241 cttgctgtat tactgactct gatttgttct tatggctttg ccagtctgtc attctatgta
    12301 ttggaacctt ggattgcagg caagaacaca cctattttac aaactcttcg tcccctgcct
    12361 tatattcacg caattcttgc agcaggtaca ggaatcttga ccttcattgt ctgcatggtg
    12421 accttgttgg caccacaagt gggagcgttt gagacagact tgactgttaa tggcttgaag
    12481 caagctgcaa caaatattgg ccagaccaag gtgatggcag aacgggcaga tgcaaacagt
    12541 ttgggaattg ctgatggcac tatgttaatt ggtgactcag tggctttaag ggcaaataca
    12601 gcactacaga cagctcttcc tggagcacag attaacgcgc aggtcagcgt aacaaccaag
    12661 accgcaaatg aaatcatgct aaataatagc cagaataaat ttttacctaa gacggtggtc
    12721 attgcgactg gggtaaataa tcctgagaat tacaaggatg actgggacag tatcgtgaaa
    12781 aatcttccta agggacaccg tatgattttg gtgactcctt atgagggaga taagacaaaa
    12841 gagacctatg ccatcgttga gaaggctgct gcctatatga gagaattggc agagaagaca
    12901 ccttacatta cgatagcaga ttggaatcaa gttgcgaaag agcatccaga aatttgggca
    12961 ggaacagacc aggttcattt cgggagtgag agtagcacta tcgaagcagg agcaaaattg
    13021 tatgcagata cgattgccac agctttgcag acagctcaag acaagccggt taaatcaaaa
    13081 taacttgtat taaaaaagag aagagttgga gaaatccagc tcttttaaaa tatctccaaa
    13141 aaataggtct ataccattta caaataaaaa agaaaggttt ataatgtaat tgacataata
    13201 aattgtagaa tcaatctttt aaggaggtta acattatgcc taactatatt aaagcggatc
    13261 agtttttcta cccacacgga gttcgtcgag gtggttactt ggaacttgtg gacggcaagt
    13321 ttgggaaaca tgtagagcag attcctgaag gggctgaggt gattgactat acaggttata
    13381 gcattgcccc aggacttgtg gatactcata ttcatggata tgcaggtgta gatgtgatgg
    13441 acaacaacat tgaaggtaca ttgcatacta tgagtgaagg acttcttagt accggtgtta
    13501 ccagtttctt acccacaact ttaacagcca cttatgagca attgcttgca gtcactgaaa
    13561 atcttggaaa ccattataaa gaagcaacag gtgctaagat tcgtgggatt tattatgaag
    13621 gtccatattt cacagaaact tttaaggggg cacaaaatcc aacttatatg agagacccgg
    13681 gtgttgagga gtttcattct tggcaaaaag cggcaaatgg cttgcttaat aaaattgccc
    13741 ttgcaccaga acgtgatggg gtggaagact ttgttcgtac agttacgggc gaaggtgtga
    13801 cggttgctct tggacattca aacgcgactt ttgatgaagc caaaaaagca gtcgatgctg
    13861 gagcgagtgt ttgggtgcat gcttacaatg gaatgcgtgg gttgactcac cgtgaattgg
    13921 gtatggttgg agccatgtac caattgccac atacctatgc agagttgatc tgtgatggtc
    13981 accacgtaga tccaaaggct tgcgaaattc ttatcaaaca aaaaggaaca gaaaacatcg
    14041 ctcttatcac agactgtatg acagctgggg gattggaaga cggagattat atgttgggag
    14101 aattcccagt tgtcgttgca aatggaactg cacgcctcaa atcgacaggt aacttggcag
    14161 gttctatcct caaactcaaa gatggtttga agaatgtggt cgaatgggga attgcgaatc
    14221 cgcatgaagc agtcatgatg gccagcttca acccagctaa atccgttcac atcgatgacg
    14281 tctgtggcca aatccgtgaa ggctacgacg ctgacttcat cgtattagat aaagatttgg
    14341 aattggtagc aacctaccta gatggcgtaa aacgttatca agcataagag agaaagcaga
    14401 agttagagac taacttctgc ttttttatgc agggtacttt actggtaaat aaaaagttaa
    14461 aaaaatcaca aaaaaagctt gaagaaacaa agaaaaaggc ttacaattga actataaata
    14521 ttacaaataa aaaaacggag gagtgcttta tgaaagccta tacttatgtt aaaccaggac
    14581 ttgcttcttt tgttgatgta gacaaaccag ttattcgcaa gccaacagat gctattgtgc
    14641 gtattgtaaa aaccactatt tgtggaacag acctccatat tatcaaaggg gatgttccta
    14701 cttgccaaag tggtaccatt cttggccacg aagggattgg gattgttgaa gaagttgggg
    14761 aaggagtttc caacttcaaa aaaggtgaca aggtcttgat ttcttgcgtc tgtgcctgtg
    14821 gtaaatgcta ctactgtaaa aaaggaattt atgctcactg tgaagacgaa gggggctgga
    14881 ttttcggtca cttgattgat ggtatgcagg ctgaatatct acgtgtccct catgcagata
    14941 atactcttta ccatactcca gaagacttgt cagatgaagc tttggttatg ctgtcagaca
    15001 ttctgcctac tggatatgaa attggtgtct taaaagggaa agtagaacct ggttgcagcg
    15061 tagccattat tggttcaggt ccagttggat tggctgctct tttaacagcc caattctatt
    15121 caccagctaa attgattatg gtagacctag acgataaccg cttggaaact gccctatcat
    15181 tcggtgcgac tcataaggtt aattcttcag accctgaaaa agccattaaa gaaatttatg
    15241 atttgacaga tggtcgtggt gtggatgtcg ctatcgaagc tgttggtatt cctgcaacat
    15301 ttgatttctg tcaaaagatt atcggtgtag acggaacggt tgccaactgt ggtgtgcatg
    15361 gtaaaccagt tgaattcgat ttagataaac tttggattcg caacatcaat gtaacaactg
    15421 gtttggtatc tacaaatacg actccacaat tgttgaaagc acttgaaagt cataagattg
    15481 aaccagaaaa attggtaact cactatttca aactcagtga aattgaaaaa gcctacgaag
    15541 tcttcagtaa ggcagcagac caccatgcca ttaaggtcat tatcgaaaac gatatctcag
    15601 aagcctaagt agtaaaaata tttttgtaca taagtaaata gaaattcagt catccatcag
    15661 atggctggat tttttatcaa aaaattaaga aatgagcata tttctttcct tgtctggcgg
    15721 aattggttat aatatacggt acaaaggaat gaatgaatat gtatcgtgtt atagaaatgt
    15781 acggagattt tgaaccgtgg tggttcttag aaggttggga agaagatatt gtagcaagta
    15841 gaaaatttga ccagtattat gatgctctca aatactacaa aacttgctgg tttagattgg
    15901 aacaagaatc ccctctttat aaaagtagaa gcgacttgat gaccattttt tgggacccgg
    15961 aagaccaacg ctggtgtgat gaatgtgatg agtatttaca acaataccat tctttggctc
    16021 ttttgcagga tgagcaggtt atcccagacg aaaaactacg ctcaggctat gaaaaacaaa
    16081 ccagtcagga aaggaatcgt tcttgccgta tgaaattaaa atagagaaaa gtaacttttt
    16141 tggagttgct ttttttattt ttctaacgct ttgcgaatag tataggtgag gaggtaagta
    16201 tggttcaaga aattgcacaa gaaatcattc gttcggctcg gaaaaaaggg gcgcaagaca
    16261 tttattttgt ccctaagtta gatgcctatg agcttcatat gagggtagga gacgagcgct
    16321 gtaaaattgg tagctatgat tttgaaaagt ttgcagccgt tatcagtcac tttaagtttg
    16381 tggcgggtat gaatgtggga gaaaaaagac gtagtcaact gggttcctgt gattatgcct
    16441 atgaccagaa gatagcgtct ctacgtttat ctactgtagg cgattatcgg gggcatgaga
    16501 gtttggttat ccgtttgttg cacgatgagg agcaggactt gcatttttgg tttcaggata
    16561 ttgaagaatt aggcaagcag tacaggcaac ggggactcta tctttttgct ggtccggttg
    16621 ggagtggtaa gacgaccttg atgcatgaat tgtccaagtc actctttaaa ggacagcaag
    16681 ttatgtccat cgaagatcct gtcgaaatca agcaggacga catgcttcag ttgcagttga
    16741 acgaagcaat cggcctaacc tatgaaaatc taatcaaact ttccttgcgt catcgaccag
    16801 atctcttgat tatcggagaa attcgtgaca gcgagacggc gcgtgcagtg gtcagagcta
    16861 gtttgacagg tgcgacagtc ttttcaacca ttcacgccaa aagtatccga ggtgtttatg
    16921 agcgtctgct ggagttgggt gtgagtgaag aagaattggc agttgttctg caaggagtct
    16981 gctaccagag attaatcggg ggaggaggaa tcgttgactt tgcaagcaga gattatcaag
    17041 aacaccaagc agccaagtgg aatgagcaaa ttgaccagct tcttaaagat ggacatatca
    17101 caagtcttca ggctgagacg gaaaaaatta gctacagcta agcaaaaaaa tatcatcacc
    17161 ctatttaaca atctcttttc tagcggtttt catctggtgg agaccatctc ctttttagat
    17221 aggagtgctt tgttggacaa gcagtgtgtg acccagatgc gtacaggctt gtctcagggg
    17281 aaatcattct cagaaatgat ggaaagtttg ggatgttcaa gtaccattgt cactcagtta
    17341 tctctagctg aagttcatgg caatcttcac ctgagtttgg gaaagataga agaatatcta
    17401 gacaatctgg ccaaggtcaa gaaaaagtta attgaagtag cgacctatcc tttgattttg
    17461 ctgggttttc ttctcttaat tatgctgggg ctacggaatt acctgctccc acaactggat
    17521 agtagcaata ttgccacccg aattatcggt aatctgccac aaatttttct aggcatggta
    17581 gggcttgttt ccgtgcttgc ccttttagca ctaacttttt ataaaagaag ttctaagatg
    17641 agtgtctttt ctatcttagc acgccttccc tttattggaa tctttgtgca gacctacttg
    17701 acagcctatt atgcacgtga atgggggaat atgatttcac agggaatgga gctgacgcag
    17761 atttttcaaa tgatgcagga acaaggttcc cagctcttta aagaaatcgg tcaagatctg
    17821 gctcaaaccc tgaaaaatgg ccgtgaattt tctcagacga taggaaccta tcctttcttt
    17881 aggaaggaat tgagtcttat catagagtat ggggaagtta agtccaagct gggtagtgag
    17941 ttggaaatct atgctgaaaa aacttgggaa gcctttttta cccgagtcaa ccgcaccatg
    18001 aatttggtgc agccactggt ttttatcttt gtggcactga ttatcgtttt actttatgcg
    18061 gcaatgctca tgcccatgta tcaaaatatg gaggtaaatt tttaaaatga aaaaaatgat
    18121 gacattcttg aaaaaagcta aggttaaagc ttttacattg attgagatgt tggtggtctt
    18181 gctgattatc agcgtgcttt tcttgctctt tgtacctaat ctgaccaagc aaaaagaggc
    18241 agtcaatgac aaaggaaaag cagctgttgt taaggtggtg gaaagccagg cagaacttta
    18301 tagcttggaa aagaatgaag atgctagcct aagcaagtta caagcagatg ggcgaatcac
    18361 ggaagaacag gctaaagctt ataaagaata ccatgataaa aatggagtag caaatcgtaa
    18421 agtcaatgat taaggccttt accatgctgg aaagtctctt ggctttgagt cttgtgagta
    18481 tccttgcctt gggcttgtcc ggctctgttc agtccacttt tgcggcagta gaggaacaga
    18541 ttttctttat ggagtttgaa gaactctatc gggaaaccca aaaacgcagt gtagccagtc
    18601 agcaaaagac taatctaaat ttagatgggc agacgcttag caatggcagt caaaagttga
    18661 cagttcctaa aggaattcag gcaccatcag gccaaagtat tacatttgac cgagctgggg
    18721 gcaattcgtc cctggctaag gttgaatttc agaccagtaa aggagcgatt cgctatcaat
    18781 tatatctagg aaatggaaaa attaaacgca ttaaggaaac aaaaaattag ggcagtgatt
    18841 ttactggaag cagtagtcgc tctagctatc tttgccagca ttgcgaccct ccttttggga
    18901 caaattcaaa aaaataggca agaggaagca aaaatcttgc aaaaggaaga agtcttgagg
    18961 gtagctaaga tggccctgca gacggggcaa aatcaggtaa gcatcaacgg agttgagatt
    19021 caggtatttt ctagtgaaaa aggattggag gtctaccatg gttcagaaca gttgttggca
    19081 atcaaagagc cataaggtca aggcttttac cttgttagaa tccctgcttg ccctcattgt
    19141 catcagtggg ggattactcc tttttcaagc tatgagtcag ctcctcattt cagaagttcg
    19201 ctaccagcaa caaagcgagc aaaaggagtg gctcttgttt gtggaccaac ttgaggcaga
    19261 attagaccgt tcgcagttcg aaaaagtaga aagcaatcgc ctatacatga agcaagatgg
    19321 caaggacatc gccatcggta agtcaaagtc agacgatttt cgtaaaaccg atgctagtgg
    19381 acgggggtat cagcctatgg tttatggcct caaatcagct cagattacag aggacaatca
    19441 actggttcat tttcgtttcc agtttcaaaa aggcttagaa agggagttca tctatcgtgt
    19501 ggaaaaagaa gaaagttaag gcaggtgttc tcctctacgc agtcaccata gcagccatct
    19561 ttagtctttt gttgcaattt tatttgaacc gacaaatcgc ccactatcaa gactatgctt
    19621 taaataaaga aaaattggtt gcttttgcca tggctaaacg aaccaaggat aaagccgagc
    19681 aagaaagtgg ggaacaggtc tttaatctag gtcaggtaag ctatcaaaac aagaaaacaa
    19741 gcttggtgac gactgttcgt acgtctaaga gccaatatga gtttctgttt ccttcagtca
    19801 aaatcaaaga agagaaaaga gataaaaagg aagaggtagc gaccgattca agcgaaaaag
    19861 tggagaagaa aaaatcagaa gagaagcctg aaaagaaaga gaattcctag tcaattcaac
    19921 tataatgcgt tgaatccaga atagtccact gtagtttcta gaaaattgct ggaaatggat
    19981 gttaagctcc aattcatttg tttatatctt atttcagttt actatacttt gtgctaaact
    20041 aaagatatga aacatgattt taaccacaaa gcagaaactt tcgactcccc taaaaatatc
    20101 ttcctcgcaa acttggtatg tcaagcagtc gagaaacaga ttgatatttt atcagacaaa
    20161 gtaattttag atttcggtgg tggcacgggt ctattagcct tgcccctagc caagcaggct
    20221 aagtcagtta ctcttgtaga catttctgag aaaatgttgg agtaagctcg tttgaaagcg
    20281 gagcagcaag caatcaagaa tatccagttt ttggagcaag atttaccgaa aaatcccttg
    20341 gagaaagagt ttgattgcct tgctgttagt cgggttcttc atcatatgcc tgatttggat
    20401 gcggatctct cactgtttca tcaacatttg aaggaagatg ggaaactcat cattgctgat
    20461 tttaccaaga cagaagctaa tcatcatgga tttgatttag ctgaactgga aaacaagcta
    20521 attgagcatg gtttttcatc tgtgcatagt cagattctct atagcgctga agacctgttt
    20581 caagggaatc actcagaatt ctttttaaca gtatcccaaa aatcactcgc ctagtcaggg
    20641 agtgattttt ctataaggat ggaaaaaaga agggaaattt gataagatag gaatatggat
    20701 tttgaaaaaa ttgaacaagc ttatatctat ttactagaga atgtccaagt catccaaagt
    20761 gatttggcga ccaactttta tgacgccttg gtggagcaaa atagcatcta tctggatggt
    20821 gaaactgagc taaaccaggt caaagacaac aatcaggccc ttaagcgttt agcactacgc
    20881 aaagaagaat ggctcaagac ctaccagttt ctcttgatga aggctgggca aacagaaccc
    20941 ttgcaggcca atcaccagtt tacgccagat gctattgcct tacttttggt atttattgtg
    21001 gaagagttgt ttacagagga ggaaattact atcctcgaaa tgggttctgg gatgggaatt
    21061 ctaggcgcta ctttcttgac ctcgcttgat aaaaaggtgg attacttggg aatggaagtg
    21121 gatgatttgc tgattgatct ggcagctagc atggcagatg taattggttt gcaggctggc
    21181 tttgtccaag gagatgccgt tcgcccacaa atgctcaaag aaagcgatgt ggtcatcagt
    21241 gacttgcctg ttggctatta tcctgatgat gccgttgcgt cgcgccatca agttgcttct
    21301 agccaagaac atacttacgc tcatcacttg ctcatggaac aagggtttaa gtaccttaag
    21361 tcagatggat atgctatttt tctagctccg agtgatttgt tgaccagtcc tcaaagtgat
    21421 ttgttaaaag aatggctgaa agaagaggcg agtctggttg ctatgattag tctgcctgaa
    21481 aatctctttg ctaatgccaa acaatctaag actattttta tcttacagaa gaaaaataaa
    21541 atagcagtag agccttttgt ttatccactt gctagcttgc aagatgcaag tgttttaatg
    21601 aaatttaaag aaaattttca aaaatggact caaggtactg aaatataaaa tagattttgt
    21661 tataatagtt gaaaacgctt aaaaaggggt atcatgttat gacaaaaaca attgcaatca
    21721 atgcaggaag ttcaagtttg aaatggcaat tatacttaat gccagaagaa aaagtattgg
    21781 cgaaaggttt gattgaacgt atcggtttga aagattcaat ttcaactgta aaatttgacg
    21841 gccgttctga acaacaaatt ttggatattg aaaatcatac acaagccgtt aaaattttat
    21901 tggatgactt gattcgtttc gatattatca aggcttatga cgagattaca ggtgttggac
    21961 atcgtgttgt tgctggtgga gaatatttca aagaatcaac agttgttgag ggagatgttt
    22021 tagaaaaagt tgaagagttg agtttgttgg ctcctctaca caacccggcc aatgcagcag
    22081 gtgttcgtgc cttcaaggaa ttgttgccag acattaccag tgtagttgtt tttgatactt
    22141 ccttccacac aagtatgcca gagaaagctt atcgctaccc tctaccaaca aaatattaca
    22201 cagaaaacaa ggttcgtaaa tacggtgctc atggtacaag tcaccagttt gtagcaggag
    22261 aagctgcaaa actcttggga cgtccattag aagacttgaa gttaattacc tgtcatattg
    22321 gtaacggagg ctcaattaca gctgtgaaag ccggcaaatc tgtagacact tctatggggt
    22381 tcactcctct tggtggtatt atgatgggaa cgcgtacagg ggatattgat ccagctatca
    22441 ttccttattt aatgcaatat acagaggatt ttaacacacc agaagatatc agtcgtgttc
    22501 ttaaccgtga atcaggtctt ttgggagttt ctgctaattc tagcgatatg cgcgatatag
    22561 aagcagctgt agcagaaggg aatcacgagg ctagcttggc ttatgaaatg tatgttgacc
    22621 gtatccaaaa acatatcggt cagtaccttg cagtgctaaa tggagcagat gccattgttt
    22681 tcacagcagg tgtcggtgaa aatgcagaga gtttccgtcg tgatgtaatc tcagggattt
    22741 cgtggtttgg ttgtgatgtt gatgatgaaa agaatgtctt tggcgttaca ggagacatct
    22801 caacagaggc agctaaaatc cgtgtcttgg ttattccaac agatgaagaa ttagtcattg
    22861 cccgtgacgt tgaacgcttg aaaaaataag tgaaactaaa aaatattcaa tacaaggagt
    22921 tgggaaagtt atttttccag cttctttttc tgatgaaatt gtccaaaacc ttgctatgat
    22981 tggctttttt gaaaaatatg gtataatagt agtaatttaa tagatggagt tgagttttga
    23041 agaaaaactt tcgtgtaaaa agagagaaag attttaaggc gattttcaag gaggggacaa
    23101 gttttgctaa tcgcaaattt gtggtctacc aattagaaaa ccagaaaaac cattttcgag
    23161 taggtctatc agttagcaaa aaactgggga atgccgtcac tagaaatcaa attaagcgac
    23221 ggattcggca tattatccag aatgcaaaag ggagtctggt agaagatgtc gactttgttg
    23281 tcattgctcg aaaaggagtc gaaaccttgg gatacgcaga gatggagaaa aatctactcc
    23341 acgtattaaa attatcaaag atttaccagg aaggaaatgg gagtgaaaaa gaaactaaag
    23401 ttgactagtt tgctaggact gtctctgtta atcatgacag cctgtgcaac taatgaggta
    23461 actagcgata ttacagccga ttcggctgat ttttggagta aattggttta cttctttgcg
    23521 gaaatcattc gctttttatc gtttgatatt agtatcggag tggggattat tctctttacg
    23581 gtcttgattc gtacagtcct cttgccagtc tttcaggtgc aaatggtggc ttctaggaaa
    23641 atgcaggaag ctcagccacg cattaaggcg cttcgagaac aatatccagg tcgagatatg
    23701 gaaagcagaa ccaaactaga gcaggaaatg cgtaaagtat ttaaagaaat gggtgtcaga
    23761 cagtcagact ctctttggcc gattttgatt cagatgccgg ttattttggc cctgttccaa
    23821 gccctatcaa gagttgactt tttaaagaca ggtcatttct tatggattaa ccttggtagt
    23881 gtggatacaa cccttgttct tccgatttta gcagcagtat tcaccttttt aagtacttgg
    23941 ttgtccaaca aagctttgtc tgagcgaaat ggcgctacga ctgcgatgat gtatgggatt
    24001 ccagtcttga tttttatctt tgcagtttat gcgccaggtg gagtcgccct atactggaca
    24061 gtgtctaatg cttatcaagt cttgcaaacc tatttcttga ataatccatt caagattatc
    24121 gcagagcgcg aggccgtagt acaggcacaa aaagatttgg aaaatagaaa aagaaaagcc
    24181 aagaaaaagg ctcagaaaac gaaataaata aggaggaatc tggtaatggt agtatttaca
    24241 ggttcaactg ttgaagaagc aatccagaaa ggattgaaag aattagatat tccaagaatg
    24301 aaggctcata tcaaagtcat ttctagggag aaaaaaggct ttcttggtct atttggtaaa
    24361 aaaccagccc aagtggatat tgaagcgatt agtgaaacga ctgttgtcaa agcaaatcaa
    24421 caggtagtaa aaggcgttcc gaaaaaaatc aatgatttga acgagcctgt gaagacggtt
    24481 agtgaagaaa ccgttgacct tggtcatgtg gttaatgcta ttaaaaaaat agaggaagaa
    24541 ggtcaaggta tttctgatga agtcaaggct gaaatcttaa aacatgaaag acatgccagc
    24601 actatcttag aagaaactgg tcacattgag attttaaatg aacttcaaat cgaggaagcg
    24661 atgagggaag aagcaggcgc tgatgacctt gaaactgagc aagatcaaac tgaaaatcaa
    24721 gacttgaaag agatgggctt gaaggtcgag caaagttatg atattgccca ggtggctacg
    24781 gatgtgactg cctatgttca agcgattgtg gatgacatgg atgttgaagc tacactttca
    24841 aatgattata accgtcgtag catcaatcta caaattgaca ccaacgaacc aggtcgtatt
    24901 atcggctacc atggtaaagt cttgaaggcc ttgcaactgt tggctcaaaa ttatctttac
    24961 aaccgctatt ccaaaacctt ctacgttaca atcaatgtca atgattatgt cgaacaccgt
    25021 gcagaagtct tgcagaccta tgcgcaaaaa ttggcgaatc gtgttttgga agaaggtcgc
    25081 agtcatcaaa cagatccaat gtcgaatagt gaacgcaaga ttatccaccg tattatttca
    25141 cgtatggatg gcgtgactag ttactctgaa ggtgatgagc caaatcgcta tgttgttgta
    25201 gatacagaat aagtaaaatc aggtttatcc tgattttttg ctagttagag gaggttaaac
    25261 tgatgttgaa taagataaga gactatttag actttgctgg tttgcagtac cgtaatcctg
    25321 ataaagcggg agcagagcga gagaagatgc tggcattccg ccacaaagga caagaggccc
    25381 gaaaggtttt tacagaactg gccaaagcct ttcaagcaag ccatccagaa tggcaactcc
    25441 aacagactag ccagtggatg aatcaggccc agcgtttgag accacatttt tgggtttatc
    25501 tacagagaga cggacaagtg acagaaccta tgatggcctt acgtttgtat gggacatcta
    25561 ctgactttgg aatttctttg gaagtcagtt tcatcgaacg taagaaggat gagcaaacac
    25621 tgggcaagca ggccaaagtt ttagacattc caaccgttaa agggatttat tatctaacct
    25681 actctaatgg tcaaagtcaa cggtgggagg cgaatgaaga aaagcgtcgt actttacgcg
    25741 agaaggtgag aagtcaagaa gttcgaaaag ttttagtgaa ggtagatgtt cctatgacag
    25801 aaaattcgtc tgaagaagaa atcgtagaag gcttattgaa gtcttattct aaaattcttc
    25861 cctattatct agctacgaga aaataagata atttgtaaaa catcataaat catacagtcc
    25921 aagagtgaac agtccgctgt gtaattcttg gtctttttgt ttgcgctttc gcattatata
    25981 ataaacttac aaaaacaatt caaaaggaga acaattatgg aagtcgtttc aagtgttcta
    26041 aattggtttt ctagcaatat tttgcagaat cccgcatttt tcgtaggttt attggtgttg
    26101 ataggatatg cacttttgaa aaaacctgcc catgacgttt tttcagggtt tgttaaagca
    26161 acagtagggt atatgttgct taacgtgggt gctggtggtt tggttacaac ctttcgtcca
    26221 atcttagcag ctcttaacta caaattccaa attggtgcag cggttatcga cccttacttt
    26281 ggacttgctg cagcaaacaa caaaattgca gcagagtttc cagattttgt tggaactgca
    26341 actacagctc tattgattgg ttttggaata aatatcttgc tcgtagctct tcgaaagatt
    26401 acgaaggtaa gaaccctctt tattactggt cacatcatgg tacaacaagc tgcaacagta
    26461 tctcttatgg ttctattctt agtaccacaa ttgcgcaatg cttacggtac agcagcgatt
    26521 ggtatcatct gtggacttta ctgggcagtt agttcaaata tgactgttga ggcaactcaa
    26581 cgcttgactg gtggtggcgg atttgcgatt ggtcaccaac agcaatttgc aatctggttt
    26641 gtagataaag tagcaggacg ctttggtaag aaagaagaaa gtttagacaa tcttaaatta
    26701 cctaagttcc tctcaatctt ccacgataca gttgttgcat ctgctacctt gatgctcgta
    26761 ttcttcgggg ccattctttt aatcttgggt ccagacatta tgtctaataa agaagtcatc
    26821 acttcaggaa ctctattcaa tcctgctaaa caagatttct ttatgtacat tatccaaaca
    26881 gcctttacct tctcagttta cttgttcgtt ttgatgcaag gtgtccgaat gttcgtatct
    26941 gagttgacaa acgccttcca aggtatttca aacaaattgt tgccaggttc attcccagcg
    27001 gttgacgttg cagcttctta tggatttggt tctccaaatg ctgtcttgtc aggatttacc
    27061 tttggtttga ttggtcaatt gattacaatt gtcttgctca tcgtctttaa aaatccgatt
    27121 cttattatta caggatttgt accagtgttc tttgacaatg cagccattgc ggtctacgct
    27181 gataaacgcg gcggatggaa agcggctgtt atcctttcct ttatatcagg tgtccttcaa
    27241 gttgctctag gagctctttg tgtggccctt ctcgatttgg catcttatgg tggctaccat
    27301 ggaaatatcg actttgaatt cccatggctt ggatttggat atatcttcaa ataccttggt
    27361 attgttggtt atgtacttgt gtgtctcttc ttgcttgtta ttcctcaact tcaatttgcc
    27421 aaagcaaaag ataaagagaa atattacaac ggtgaagttc aagaagaagc ttagtatcta
    27481 gaaaaggaga aataaaatgg ttaaagtatt agcagcgtgc ggaaatggaa tgggttcatc
    27541 aatggttatc aagatgaagg ttgaaaatgc tctccgtaag cttaatcaaa cagattttac
    27601 agtcaattca tgcagtgtcg gtgaagctaa aggtttagca gtaggatatg acatcgtaat
    27661 cgcttctctt catttgattc aagaattgga agggcgaact aatgggaagt taattgggct
    27721 tgataacttg atggatgata aagaaatcac cgaaaaactc agtcaagcac tacagtaaaa
    27781 ggttggaggg ggctggacag aaactgagag ttatcgtttc tgtccttctc cctctttaaa
    27841 taaaggaggc agatatgaat ttaaaacaag ctttaattga caacgactcg atccgactag
    27901 gtttagaggc taacgattgg aaagaagcag tcaaggtagc agtagatccc ttgattgaaa
    27961 gtggggcaat tttgccagag tattacgatg ctatcattga atcgactgaa gagtatgggc
    28021 cttactatat cttgatgcca ggtatggcta tgccccacgc tagacctgaa gctggtgtgc
    28081 aaagtgatgc cttttcattg attaccttac aaaatcctgt tgtattttca gatgggaaag
    28141 aggtatctgt tttgttggca ctagcagcaa caagctcaaa aattcacaca agtgtagcca
    28201 ttccacaaat tattgccctg tttgaattag aagattctat tgcacgttta caggcttgcc
    28261 agactaaaga agatgtcttg gctatgattg aagaatctaa ggatagccct tatctcgaag
    28321 gattggactt ggaaagttag aaagaggaat aaagaaatga caaaaagaat acctaattta
    28381 caagttgcat tagaccattc agacttgcaa ggagcgatta aagcagctgt ttctgttagt
    28441 caggaagtag atattatcga agctggaact gtttgcttgc ttcaagttgg aagtgaactg
    28501 gctgaagtct tgcgtagcct tttcccagat aagattattg tggcagacac aaaatgtgct
    28561 gatgctggtg gaacagttgc taaaaataat gcggttcgtg gagcagactg gatgacttgt
    28621 atctgttgtg caaccatccc tactatggaa gcagctctaa aggctatcaa gactgaacga
    28681 ggagaacgag gcgaaatcca gatcgagctt tatggcgatt ggacttttga acaagctcag
    28741 ctttggctag atgcaggtat ctcacaagct atttatcacc aatctcgtga tgctcttctt
    28801 gctggtgaaa cttggggtga aaaagacctt aataaggtta aaaaactcat tgacatgggc
    28861 ttccgtgtat ctgtaacagg tggtctagat gtagatactc tcaaactctt tgaaggtgtt
    28921 gatgtcttta cctttatcgt aggtcgtgga attacagagg ctgtggatcc agcaggagca
    28981 gcgcgtgcct tcaaggatga aatcaaacga atttgggggt aaatcatggt acgtccaatt
    29041 ggaatttatg aaaaggcaac cccaacacac tttacttggc tagaacgttt aaattttgcc
    29101 aaggagttag gctttgattt tgtcgagatg tctattgacg aacgtgacga gcgtttagga
    29161 agacttgact ggagtaagga agaacgcttg gaagttgtca aagcaatcta tggaactggt
    29221 gttcgtattc cttctatctg tttttcaggc catcgtcgct acccattggg ttcaaaagat
    29281 ccagttctag aggaaaaatc tctagaactc atgaaaaaat gtatcgaatt agctcaagac
    29341 ttgggagttc gtacgattca attagctggt tacgatgttt actatgagga aaagtcaccc
    29401 cagacacgcc aacgttttat caaaaatttg agaaaagcct gtgactgggc tgaagaagct
    29461 caggtggtac ttgctattga aattatggat gatcctttca tcaatagcat cgaaaaatat
    29521 ttggctatag aaaaagagat tgactctccc ttcctctttg tatatccaga tattggtaat
    29581 gtgtctgcat ggcataatga tatctatagt gagttttatc ttggtcatca tgccatcgca
    29641 gctctccatc tcaaggatac ttatgcagtg acagaaagtt caaagggcca gttccgagat
    29701 gtaccttttg ggcaaggttg tgtcaaatgg gaagaagctt tcgatatttt aaaggaaacc
    29761 aattataatg gacctttcct aatcgaaatg tggtctgaaa attgtgaaac agtagaagaa
    29821 acacgcgcag ccattcaaga ggcgcaagct tttctctatc cactcattaa gaaagcaggt
    29881 ttgatgtaag atgaatcaag taatcaatgc tatgcgtaaa cgagtctgtg atgccaatca
    29941 atcattgcca aaacatggac ttgtcaaatt tacctggggg aatgtatctg aagtcaatcg
    30001 agaactcggt gtcattgtta tcaaaccatc aggcgtggat tatgacgaat tgacacctga
    30061 aaacatggta gtgactgatc tagatggtaa gatcctagaa ggggatttaa gaccatcttc
    30121 cgacctccca actcatgtgc aattatataa ggcttggtca gaaattggta gtgtggttca
    30181 cacccattcg acagaagctg ttgcttgggc tcaggcaggt cgtgatattc ctttctacgg
    30241 aacaacccat gcagattatt tctacggttc aatcccttgc gcccgtagtt tgaccaagga
    30301 cgaagtagaa gtggcctatg aaaaagatac tggcctggtt atcgtagaag agtttgaaca
    30361 tcgcggactt aacccggttg aagtaccagg aattgttgta cgcaatcacg gtccattcac
    30421 ctggggcaaa aatccagaga atgctgttta tcactctgtc gtactagagg aagtatcaaa
    30481 gatgaatcgc tttacagaac aaatcaatcc aagagttgaa cctgctcccc agtacatact
    30541 agaaaaacac taccaacgta aacatggacc aaatgcttat tatggtcaaa agtaagaacg
    30601 atgaaggagg agaaaaagat aaatttagct cctcttttta catttgattt ttattgagag
    30661 taaagttgga gttgaagtaa ttttgaaaga ttttttagaa atagcgcttg atatatatgg
    30721 taaaataaaa agaattgctg tgatatcaat agatttgggg gattttttaa tatggtactg
    30781 gataaggcaa gttgtgattt gcttcaatat ttgatggatc aagaaacgtc caaaacgatt
    30841 atggcgattt cgaaagattt gaaagagtca agaaggaaaa tttattatca cattgacaaa
    30901 atcaatgctg ctctggttga cgaggcgctt cacatcatta gtattccacg aattggtatt
    30961 cacttaacgg aagagcagag agatgcttgt tgtaaactat tatcggaagt agattcgtac
    31021 gattatatca tgagtgcgca tgaacgtatg atgataatgt tactatggat aggtatttct
    31081 aaagaacgta ttactattga aaaattgata gagttaacag aggtatctag gaatactgtt
    31141 ctcaatgatt tgaatagtat tcgttatcaa ctaactttgg aacaatatca ggtgaccttg
    31201 caagtgagca agtcacaggg ataccacctt catgcccacc ctcttaataa aattcagtat
    31261 cttcaatcgc ttctatatca tatttttatg gaagaaaatg ccacttttgt atctatttta
    31321 gaagataaga tgaaagagag gttagatgat gagtgtttgc tttctgttga aatgaaccaa
    31381 ttttttaagg aacaggttcc tttagttgaa caagatttag ggaagaaaat aaaccatcat
    31441 gaaataactt ttatgttgca ggttctacct tatttgctgt taagctgtca taatgttgaa
    31501 cagtatcaag aaagacatca ggatatagag aaagaatttt ctttgataag aaaaagaata
    31561 gagtatcagg tgtctaagaa attaggagaa cggttgtttc aaaagtttga aatttctttg
    31621 tcaggacttg aagtttctct tgtagctgtt ctcctcctct cctatcgtaa agatttggat
    31681 attcatgcag aaagtgatga ttttcggcaa ttaaaacttg ctttagaaga atttatctgg
    31741 tattttgaat cacaaatccg aatggagatt gagaacaagg atgatttgtt acgaaatttg
    31801 atgatccact gtaaagcctt gttatttaga aagacttacg gtattttttc taaaaatcct
    31861 ctaacaaaac aaattcgatc caagtatgga gaattatttt tagtcactag aaaatctgcg
    31921 gaaattttag aaggagcatg gtttattcgg ctaacagacg atgatattgc ctatttgacg
    31981 attcatattg gaggattttt aaagtataca ctatcgtctc aaaaaaatat gaaaaaagtt
    32041 tatctcgttt gtgatgaagg tgttgcggtt tcgagacttt tgctgaaaca atgcaaactt
    32101 tattttctaa atgagcaaat tgacactgta tttacaacag aacaatttaa gagtgtggaa
    32161 gatattgcac aagttgatgt agtgattact actaatgatg atttggatag cagatttccg
    32221 attttaaggg ttaatcctat ccttgaagca gaagatattt tgaaaatgct agactatctt
    32281 aaacacaata tatttcgtaa taagagcaaa agtttcagtg aaaatctttc tagtcttatt
    32341 tcgtcttata ttgtagacag caagttggct agtaagttcc aagaagaggt tcaaacactt
    32401 ataaatcaag aaatagtagt tcaagctttt ttggaagata tttgaaggac agtccaatga
    32461 tgaacacaaa cctgtgtttt tcttggtctt ttttagtgtt ttgaagggtg gtatactaat
    32521 ctcaaagata acaattatat ccaaaggagg caacatatgc caaacgtcaa agaaattaca
    32581 agagagtcat ggattttagc cactttccca gagtggggaa catggttgaa cgaagaaatc
    32641 gaagaagaag tcgtacctga aggcaacttt gccatgtggt ggctaggcaa ctgtggtact
    32701 tggattaaga caccagctgg tgctaacgtt gtcatggacc tttggtcaaa ccgtggaaaa
    32761 tcaaccaaaa aagtgaaaga tatggttcgt gggcaccaaa tggcaaatat ggcaggtgtt
    32821 cgtaagctgc aaccaaactt gcgtgttcag ccaatggtta tcgatccatt tgctatcaac
    32881 gaactagact attacttagt ttcacacttc cacagtgatc atatcgaccc atacacagct
    32941 gcagcaattc tcaataatcc taagttagag catgttaagt ttatcggtcc ttaccactgt
    33001 ggacgaatct gggaaggatg gggtgttcca aaagaacgta tcatcgttgt taaaccaggt
    33061 gacactatcg aattaaaaga tatgaagatt catgcagtag aatcatttga ccgtacttgc
    33121 ttggtaactc tcccagtgaa cggtgctgat gagacaggcg gtgaacttgc tggcttggct
    33181 gttacagatg aagaaatggc tcaaaaggct gttaactata tctttgaaac accaggtgga
    33241 accatctatc atggtgcaga ttctcacttc tcaaactatt ttgcaaaaca tggtaaagac
    33301 tttaaaattg atgttgcttt gaataactat ggtgaaaatc cggtaggtat ccaagacaaa
    33361 atgacatcta tcgaccttct tcgtatggca gaaaatctgc gtaccaaagt cattatccca
    33421 gttcactatg atatctggtc taacttcatg gcttctacta atgagattct agaactttgg
    33481 aaaatgcgaa aagatcgctt gcaatacgat ttccatccat ttatctggga agttggcggt
    33541 aagtacactt atcctcaaga tcaacactta gtagaatacc atcatccacg tggttttgat
    33601 gattgttttg aacaagactc taacattcaa tttaaagctt tgctataata taaaaatatt
    33661 ctttggaggt tatccgtatg ttgcatgata cggataattt tcatataata gtaaaataga
    33721 atgtgtgatt caataatcac ctcaaataga aaggaaattc tatgtcaaat ctatctgtta
    33781 atgcaattcg ttttctaggt attgacgcca ttaataaagc caactcaggt catccaggtg
    33841 tggttatggg agcggctccg atggcttaca gcctctttac aaaacaactt catatcaatc
    33901 cagctcaacc aaactggatt aaccgcgacc gctttattct ttcagcaggt catggttcaa
    33961 tgctccttta tgctcttctt cacctttctg gttttgaaga tgtcagcatg gatgagatta
    34021 agagtttccg tcaatggggt tcaaaaacac caggtcaccc agaatttggt catacggcag
    34081 ggattgatgc tacgacaggt cctctagggc aagggatttc aactgctact ggttttgccc
    34141 aagcagaacg tttcttggca gccaaatata accgtgaagg ttacaatatc tttgaccact
    34201 atacttacgt tatctgtgga gacggagact tgatggaagg tgtctcaagc gaggcagctt
    34261 catacgcagg cttgcaaaaa cttgataagt tggttgttct ttatgattca aatgatatca
    34321 acttggatgg tgagacaaag gattccttta cagaaagtgt tcgtgaccgt tacaatgcct
    34381 acggttggca tactgccttg gttgaaaatg gaacagactt ggaagccatc catgctgcta
    34441 tcgaaacagc aaaagcttca ggcaagccat ctttgattga agtgaagacg gttattggat
    34501 acggttctcc aaacaaacaa ggaactaatg ctgtacacgg cgcccctctt ggagcagatg
    34561 aaactgcatc aactcgtcaa gccctcggtt gggactacga accatttgaa attccagaac
    34621 aagtatatgc tgatttcaaa gaacatgttg cagaccgtgg cgcatcagct tatcaagctt
    34681 ggactaaatt agttgcagat tataaagaag ctcatccaga actggctgca gaagtagaag
    34741 ccatcatcga cggacgtgat ccagtcgaag tgactccagc agacttccca gctttagaaa
    34801 atggtttttc tcaagcaact cgtaattcaa gtcaagatgc cttgaatgtt gtggcagcta
    34861 aattaccaac ctttctaggt ggatcagctg accttgctca ctcaaacatg acttatatca
    34921 agacggatgg acttcaagac gatgccaatc gcttgaatcg taacattcag tttggtgttc
    34981 gtgaatttgc aatgggaaca atcttgaacg ggatggccct tcatggtgga cttcgtgtat
    35041 acggtggaac tttcttcgtc ttctctgact atgtgaaggc agctgtccgc ttgtcagcct
    35101 tacaaggact tcctgtgact tatgtcttta cccatgattc aatcgcagtt ggggaagatg
    35161 gtccgactca tgaaccagtt gagcatttag caggtcttcg tgctatgcca aatctaaatg
    35221 ttttccgtcc agcagatgcg cgtgaaacgc aagcagcttg gtaccttgca gtgacaagtg
    35281 agaaaacacc aactgccctt gtcttgacac gtcaaaattt gactgttgaa gatggaacag
    35341 acttcgacaa ggttgctaaa ggtgcttatg ttgtatatga aaatgcagcc gactttgata
    35401 ccatcttgat tgcgacaggt tcagaggtta atcttgctgt ctcagctgct aaagaattgg
    35461 ctagtcaagg cgaaaaaatc cgcgtagtca gcatgccatc tacagatgtc tttgataaac
    35521 aagatgcagc ttacaaggaa gaaattcttc caaatgcagt ccgccgtcgt gttgcagtcg
    35581 aaatgggtgc aagtcaaaac tggtacaaat atgttggtct cgatggtgcc gttctaggta
    35641 ttgatacctt cggagcctct gccccagcac caaaagtatt ggcagaatat ggctttactg
    35701 tagaaaatct tgtaaaagtt gttcgaaact tgaaataatc ctaaaaatca gggcgtaagc
    35761 tctggttttt cttaccagaa aagtaaggta caatcttgta aaagtagctg aaatttgata
    35821 tagtagtcct atgtaaaaga caaaggagaa aacaatgaat ccaaatatta cttttttaat
    35881 catgcttgta ggtatgatgg ccttgatgtt ctttatgcaa cgttctcaaa agaaacaagc
    35941 tcaaaagcgt atggaaagct taaataaact acaaaaaggc tatgaagtga ttacaatcgg
    36001 tggactttat ggaacagtcg atgaagtaga tacggagaag ggaacaatag ttcttgacgt
    36061 agatggagtt tacttgactt ttgaactagc tgctatcaag acagtattac cgctgaaaga
    36121 aacagcttca ctagaaggcg caattgaaaa ataagacggg atcacgaact cccgttttct
    36181 ataaaagaaa ggaaatggga tgaaaaaatt agtctttgtc tgtctgggaa atatttgccg
    36241 tagccctatg gccgagtttg ttatgaaatc aatgacagat aactacgaaa tccaaagtcg
    36301 agcaacttcc tcttgggaac atggcaatcc gattcataag gggactcagg gaatttttca
    36361 agagtatgag attccttatg acaagaacaa gacatcgctt cagattagta aggaagattt
    36421 tgaagccttt gattatatta tcggaatgga cgcttcaaat atttctgact tacgtcagat
    36481 gtgtccagta gactgtcaag ataagattta ctcattttca tctgaaagtg ttccagaccc
    36541 ttggtataca ggagattttg aagaaaccta tcgacgtgtt caagagggct gtcaagcttg
    36601 gttagaacgt ttagaaaagg agagttaagg tggaaaatct tacgaatttt tacgaaaagt
    36661 atcgtgtcta tctgactcgt ccacgtttag agcttttggc agtagttacc attgttttct
    36721 gtgctgtact cgtctttttt ctaaatattc caggaaaagg tgtcttaaaa ctcgataatg
    36781 gaacgattgt ttatgatggc agtcttgtcc gtggtaaaat gaatggccaa ggtaccatta
    36841 ccttccaaaa tggagaccaa tatacaggtg gcttcaacaa tggagccttc aacggaaaag
    36901 gtacctttca atctaaagaa ggctggacct acgaaggtga ttttgtaaat ggtcaggctg
    36961 aaggaaaagg gaaactaaca acagaacaag aagtcgttta tgaaggaact tttaaacaag
    37021 gcgtttttca acaaaaataa agcctcctta tcaaaggagg tattattaga atcacaaggt
    37081 aagcgcttac ctgtaaatcc ctttctttcc aaatccctct tccaagcaag tttgtgaaat
    37141 aaaaaatatt tgaaataaat ttcacaaact tcaaagataa aacttgataa gaaaagaaaa
    37201 tgagaaaagt ttcacaagag tttaaaaatt ctttgtgaaa tgtttatgaa actgtttaca
    37261 aagttgaaat aatgcgttat aataaacttg tgaaaaaatt aacaaaggat atcgttcctt
    37321 gaaagctatg gaggaaaata tggctgataa aaaaactgtg acaccagagg aaaagaaact
    37381 cgttgctgaa aaacacgtag atgagttggt tcaaaaagct ctagttgccc ttgaagaaat
    37441 gcgtaaattg gatcaagaac aagttgacta catcgttgcc aaagcatcag tagcagcttt
    37501 ggatgcccac ggagaattgg ctttacatgc ctttgaagaa acaggacgtg gtgtatttga
    37561 agacaaagca actaagaact tgtttgcctg tgaacacgta gtaaacaaca tgcgccacac
    37621 taagacagtt ggcgttatcg aagaagacga tgtaacagga ttgactctta ttgctgaacc
    37681 agttggtgtt gtttgtggta ttactccaac aacaaaccca acatcaacag caatcttcaa
    37741 atcattgatt tcattgaaga cacgtaaccc aatcgtcttt gccttccatc catcagcaca
    37801 agaatcatct gctcatgcag ctcgtatcgt ccgcgatgca gctatcgcag ctggtgctcc
    37861 tgaaaactgt gtgcaatgga ttactcaacc atctatggaa gcaacaagtg cccttatgaa
    37921 ccacgaaggt gttgcgacaa tccttgcaac aggtggtaat gccatggtta aggcggctta
    37981 ttcatgtggt aaaccagctc ttggggtagg tgccggaaac gttccagctt atgttgaaaa
    38041 atcagcaaac attcgtcaag cagcacacga tatcgtcatg tctaaatcat ttgataacgg
    38101 tatggtctgt gcatctgaac aagcagttat cattgataaa gaaatttacg atgaatttgt
    38161 agcagagttc aaatcttacc acacttactt tgtaaacaaa aaagaaaaag ctcttcttga
    38221 agagttctgc ttcggcgtca aagcaaacag caaaaactgt gctggtgcaa aattgaacgc
    38281 tgacatcgtt ggtaaaccag caacttggat tgcagaacaa gcaggattta cagttccaga
    38341 aggaacaaac attcttgctg cagaatgtaa agaagttggc gaaaatgagc cattgactcg
    38401 tgaaaaattg tcaccagtta ttgcagtttt gaaatctgaa agccgtgaag atggtattac
    38461 taaggctcgt caaatggttg aatttaacgg tcttggacac tcagcagcta tccacacagc
    38521 tgacgaagaa ttgactaaag aatttggtaa agctgttaaa gctattcgtg ttatctgtaa
    38581 ctcaccttct acttttggtg gtatcgggga cgtttacaat gccttcttgc catcattgac
    38641 acttggatgt ggttcttacg gacgcaactc agttggggat aacgttagtg ccattaacct
    38701 cttgaatatc aaaaaagtcg gaagacggag aaataacatg caatggatga aacttccttc
    38761 aaaaacatac tttgaacgtg attcaattca ataccttcaa aaatgtcgtg acgttgaacg
    38821 tgtcatgatc gttactgacc atgccatggt agagcttggt ttccttgatc gtatcatcga
    38881 acaactggac cttcgtcgca ataaggttgt ttaccaaatc tttgcggatg tagaaccgga
    38941 tccagatatc acaactgtaa accgtggtac tgagattatg cgtgccttca aaccagatac
    39001 catcatcgca ctcggtggtg ggtctccaat ggatgctgcc aaagtaatgt ggctcttcta
    39061 cgagcaacca gaagtggact tccgtgacct tgtccaaaaa ttcatggata tccgtaaacg
    39121 tgccttcaag ttcccattgc ttggtaagaa gactaaattc atcgcgattc caactacatc
    39181 tggtacagga tctgaagtaa caccatttgc cgttatctct gataaagcaa acaaccgtaa
    39241 atacccaatc gctgactact cattgacacc aactgtggca atcgtagatc ctgctttggt
    39301 attgacagtt ccaggatttg ttgctgctga tactggtatg gacgtattga ctcacgcgac
    39361 agaagcatac gtatcacaaa tggctagtga ctacactgat ggtttagcac ttcaagccat
    39421 taaattggtc tttgaaaatc tcgaaagctc agttaagaat gcagacttcc actcacgtga
    39481 gaaaatgcat aacgcttcaa caatcgctgg tatggccttt gccaatgcct tcctaggtat
    39541 ttctcactca atggcccata agattggtgc gcaattccac acaatccacg gtcgtacaaa
    39601 tgctatcttg cttccatacg ttatccgtta caacggtaca cgtccagcta agacagcaac
    39661 atggcctaag tacaactact accgtgcaga tgaaaaatac caagatatcg cacgcatgct
    39721 tggacttcca gcttctactc cagaagaagg ggttgaatct tacgcaaaag ctgtctacga
    39781 actcggtgaa cgtattggga tccaaatgaa ttttagagac caaggaattg acgaaaaaga
    39841 atggaaagaa cattctcgtg aattagcctt cctggcttat gaagaccaat gttcaccagc
    39901 taacccacgt cttccaatgg tagaccatat gcaagaaatc atcgaagatg catactatgg
    39961 ctacaaagaa agaccaggac gccgtaaata attgtttatc agtctagaag caagacaaaa
    40021 actcaatttg agggaaagat ccagtaattt ttctatgata aaaggcatcc tatcaaggtt
    40081 tttgaacacc tgataggatg cctttttatg atattgaggc ctttttgccc tttttgaaaa
    40141 actagaatag aaacaaaata tataatagat tgaaactgga atagtacact tctgcttcta
    40201 aaacattgtt agaattcgat ttgactgtcc tgatcgattt gtcctattcc tatttcattt
//
LOCUS       DAVKGC010000005        37926 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKGC010000005 DAVKGC010000000
VERSION     DAVKGC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.005"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..7023
                     /locus_tag="VW049_000215"
     CDS             <1..7023
                     /locus_tag="VW049_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9431403.1"
                     /translation="HSHKKSFDWYSMQQRYSIRKYYFGAASVLLGTALVLGAAASVQT
                     VQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTEEVTK
                     PAEKVEEAKDKKEEVTHQDAVDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAGQS
                     LLGKADATEAELSAAESSIQSFIIGLELRSNSNKETVSETPVAKKADAVESKEGAKPA
                     ATTERSAVDSAILPTSTADKVETTSAPASINEILKLGLSLSDARQNPAIRKEDVNRGY
                     SGFRAASNPANPIVSGSGNTVAFADISQGGRSYSFRGYGNSRGGNSIHYDVTTVRSGN
                     SVNFTISYSAPGDSREFVNNNFILDKGDGFGNPSNATITSSNPIVREQSKSISQGANY
                     VSHSGYSMTSAISTNTEQTIRFSLPIINLNGDLSVRLKPVTFNVDQGGGGAATSNDPY
                     SNSNYYYRANPLYLDANPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQ
                     RQITYKVHRFGNETLLGLPISNSVTKEAKPRIMQIGVAKDLIDTVKPRVDQNKVGDTN
                     NLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGAQGERGLTGAQGEKGDRGERGL
                     TGAQGAKGEKGAQGERGLTGAQGAKGEKGDQGERGLTGAQGAKGEKGDQGERGLTGAQ
                     GAKGEKGAQGERGLTGTQGAKGEKGDRGERGLTGAQGAKGEKGARGERGLTGAQGAKG
                     EKGAQGERGLTGAQGAKGEKGDQGERGLTGAQGEKGDRGERGLTGAKGEKGDQGERGL
                     TGAQGEKGDRGERGLTGAKGEKGAQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGN
                     VTSTVVRDGFDGASPLVATQRNDADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGA
                     KGEKGDKGEQGLQGRDGEQGPKGEDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVR
                     DGFDGASPLVSTHRNEADKTTTVIFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDK
                     GDNGKDGFTPEVTVTDNNNGTHTITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAK
                     KQTTLTFYIDKDGDGSYTAGKDELVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTE
                     GKDGDTFVNTQTGDVFVKKGNTWEPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQG
                     VKGEKGDQGERGLTGSKGEKGAQGERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQ
                     RGPAGPQGPKGEQGNPGTPGKDGKSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATI
                     TDGRNGTNGQSPTITATRSVQNGKNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVT
                     ITEGQNGTHTLTVHNPGSPDVTTTIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKY
                     INTETGDVYVKNNGNWDKEGNIKGPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGA
                     VGRDGRDGKDVLNGKANPEAHQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDK
                     GERGEDGKTPEVTVTPGKDGHSTDITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRV
                     QGKNGNPSHTIVTFYTDENNDGKYTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDG
                     KETKVYQEDPANPGQPLNPEKPLAVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHD
                     GSQPTTVFVQDGAKGETGATGQDGQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKD
                     GKNGKDGRAPKIKVEDITSPSRIRRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDK
                     VLNSKDIYNGIDGRDGSAPTITTKDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANI
                     TTTENPDGSHTITVTNPDGSTKETVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNV
                     TNAIIKDGKDGKAATATTTENPDGSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASV
                     RDNGDGSHTIVITNPEGVTTETTVRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTV
                     RDGREPKLEVIDNNDGSHTIKVTGADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVD
                     SDGREYKSIIKDGKDGKDSVSPTVTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKS
                     PKVSVEDNGDGSHTITIINSDGTVTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKP
                     VTPSNDKPVPPTPNVPTPEVPVKPVPAVPEQPVVPTPAQPATPVNANPVAPTTGKENR
                     GDKLPETGSQSDYISVLLGSGILLSLYVGRRKEA"
     gene            complement(7260..7808)
                     /locus_tag="VW049_000216"
     CDS             complement(7260..7808)
                     /locus_tag="VW049_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9431404.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            8129..8617
                     /locus_tag="VW049_000217"
     CDS             8129..8617
                     /locus_tag="VW049_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9431405.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            8614..9801
                     /locus_tag="VW049_000218"
     CDS             8614..9801
                     /locus_tag="VW049_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9431406.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            9794..10159
                     /locus_tag="VW049_000219"
     CDS             9794..10159
                     /locus_tag="VW049_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9431407.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            10175..11521
                     /gene="asnS"
                     /locus_tag="VW049_000220"
     CDS             10175..11521
                     /gene="asnS"
                     /locus_tag="VW049_000220"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9431408.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            11674..11964
                     /locus_tag="VW049_000221"
     CDS             11674..11964
                     /locus_tag="VW049_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9431409.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            11976..12446
                     /gene="ssbA"
                     /locus_tag="VW049_000222"
     CDS             11976..12446
                     /gene="ssbA"
                     /locus_tag="VW049_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9431410.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            12478..12717
                     /locus_tag="VW049_000223"
     CDS             12478..12717
                     /locus_tag="VW049_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9431411.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            13012..14412
                     /locus_tag="VW049_000224"
     CDS             13012..14412
                     /locus_tag="VW049_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9431412.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVYLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            14414..14773
                     /locus_tag="VW049_000225"
     CDS             14414..14773
                     /locus_tag="VW049_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9431413.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            14827..15576
                     /locus_tag="VW049_000226"
     CDS             14827..15576
                     /locus_tag="VW049_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9431414.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPHSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            15566..15865
                     /locus_tag="VW049_000227"
     CDS             15566..15865
                     /locus_tag="VW049_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9431415.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            15936..16871
                     /locus_tag="VW049_000228"
     CDS             15936..16871
                     /locus_tag="VW049_000228"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9431416.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNELQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            16958..17470
                     /locus_tag="VW049_000229"
     CDS             16958..17470
                     /locus_tag="VW049_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9431417.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            17507..18024
                     /locus_tag="VW049_000230"
                     /pseudo
     CDS             17507..18024
                     /locus_tag="VW049_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850249.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            18125..18319
                     /locus_tag="VW049_000231"
     CDS             18125..18319
                     /locus_tag="VW049_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9431418.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(18365..19810)
                     /locus_tag="VW049_000232"
     CDS             complement(18365..19810)
                     /locus_tag="VW049_000232"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9431419.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGTTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            19916..21538
                     /locus_tag="VW049_000233"
     CDS             19916..21538
                     /locus_tag="VW049_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9431420.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLAMGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKVQADRLRAKFKLS"
     gene            21743..23701
                     /locus_tag="VW049_000234"
     CDS             21743..23701
                     /locus_tag="VW049_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9431421.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRATTNDIVTNLVDGLMENDQYGNYVPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGI
                     KALDDQTVQYTLARPEPYWNSKTTSTILFPVNADFLKSKGDDFGKVDPANILYSGPFL
                     MKAFVSKSVIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTKMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            23873..25555
                     /locus_tag="VW049_000235"
     CDS             23873..25555
                     /locus_tag="VW049_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431422.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVTEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTVYSRSKGSSVALENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENR
                     PVLKDITCSIFKGQKIAFVGPSGSGKSTIVRLLEQFYKPLSGDILMEQSSIYDFNLKE
                     WRSKIAWVSQNNAVLSGSIRDNLCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDT
                     EVGERGRLLSGGRSQRLQIARAYLKDAEILIFDEATANLDADSEYAIISSLYSVLKEK
                     TVVIIAHRLSTVKDVDCIFFLEEGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            25713..26807
                     /locus_tag="VW049_000236"
     CDS             25713..26807
                     /locus_tag="VW049_000236"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9431423.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            26815..27981
                     /locus_tag="VW049_000237"
     CDS             26815..27981
                     /locus_tag="VW049_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9431424.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IHQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            28074..31172
                     /locus_tag="VW049_000238"
     CDS             28074..31172
                     /locus_tag="VW049_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9431425.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLIFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMCLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGPVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVTVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            31220..31837
                     /locus_tag="VW049_000239"
     CDS             31220..31837
                     /locus_tag="VW049_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9431426.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTAPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            31848..33182
                     /locus_tag="VW049_000240"
     CDS             31848..33182
                     /locus_tag="VW049_000240"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9431427.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSITGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            33192..33656
                     /locus_tag="VW049_000241"
     CDS             33192..33656
                     /locus_tag="VW049_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9431428.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            33653..34147
                     /locus_tag="VW049_000242"
     CDS             33653..34147
                     /locus_tag="VW049_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9431429.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            34228..35883
                     /gene="mltG"
                     /locus_tag="VW049_000243"
     CDS             34228..35883
                     /gene="mltG"
                     /locus_tag="VW049_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9431430.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            35954..36436
                     /gene="greA"
                     /locus_tag="VW049_000244"
     CDS             35954..36436
                     /gene="greA"
                     /locus_tag="VW049_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9431431.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            36990..37388
                     /locus_tag="VW049_000245"
     CDS             36990..37388
                     /locus_tag="VW049_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9431432.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            37809..>37926
                     /locus_tag="VW049_000246"
     CDS             37809..>37926
                     /locus_tag="VW049_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9431433.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGK"
BASE COUNT        11937 a         7014 c         8555 g        10420 t
ORIGIN      
        1 cattcacata aaaaatcatt tgactggtat agcatgcaac aacgttattc tattcgtaag
       61 tattactttg gtgcagctag tgtcttgctc ggtaccgctt tggtattagg tgcagcagct
      121 agtgtccaaa cagtacaagc ggaagaaaac aaacaagaaa ctaccaatag tatttctgtt
      181 ggtaggggag aagcagctac taaaccagca gaggtttctg cgtctaataa agagaaaacc
      241 tatgcagctc caactgtagc taatccagta gaaacgactc cagttaaaac tgaagaagtt
      301 actaaaccag cagaaaaagt tgaagaagca aaagacaaaa aagaggaagt aacgcatcaa
      361 gatgccgttg acaagtcaaa attattaacg gctctttcgc gtgctaaaaa attagaaagc
      421 aagttatata cagaagcaag tgctgctaac ttgcaaacaa gtatccaagc tggtcaaagc
      481 ttgcttggaa aagcagatgc aactgaagct gaattatcag cagcagagtc atctattcaa
      541 tcatttatta ttggtctaga acttcgttct aactctaata aagaaactgt atcagaaacg
      601 cctgtagcga agaaagctga tgcagttgaa tcaaaagaag gggctaaacc agctgcaaca
      661 actgaacgtt cagctgttga tagcgctatt ttgccaacta gcacagctga caaagtagaa
      721 acaacttcag ctccagcatc tattaatgaa atcttgaaac taggtttgag cctttctgat
      781 gctcgccaaa atccagctat ccgtaaggaa gatgttaata gagggtatag tggttttaga
      841 gcggctagca atccagccaa cccaattgtc tcaggttctg gaaatacagt tgcatttgca
      901 gatattagcc aaggtggtcg tagctatagt ttccgtggct acgggaactc acgtggtgga
      961 aattctattc attacgatgt aacaacagta cgtagtggta atagtgttaa ctttacaatt
     1021 agttattctg cgccaggaga ttctagagag tttgttaata ataattttat cttggataaa
     1081 ggggatggat ttggaaatcc ttcaaatgca acgatcacaa gttcaaatcc aatagtaagg
     1141 gagcaatcaa aatctattag tcagggtgcc aactacgtat ctcactcagg atacagtatg
     1201 acctctgcta tttcaacaaa tacggaacaa actatcagat ttagtttgcc tatcattaat
     1261 ctaaatggtg atttgtctgt tcgtttgaaa cctgttactt ttaacgtgga ccaaggtggt
     1321 ggtggtgccg ctactagcaa tgacccatac agtaactcta actattatta tagagcaaac
     1381 ccactatact tggatgcaaa cccttatggt ggtactaata ataagactgt ttcagaagac
     1441 attgacttcc aaactgtcta tcttccaact agtaagttac cagaaggtca aactagatta
     1501 gttcgagaag gtgaaaaagg acaacgtcaa attacctata aagttcatcg atttggtaac
     1561 gaaacacttt taggattgcc gattagtaat agtgttacta aagaagctaa gccacgtatt
     1621 atgcaaattg gtgtggctaa agatctaatc gatacagtaa aaccacgtgt tgatcaaaat
     1681 aaagtcggtg atacaaataa cctcactttc tatcttgata acgatggaaa cggtgtttat
     1741 actgaaggtg tagacgaact tgttcaaaaa attgctatta aagatggagc taaaggtgaa
     1801 aaaggagccc aaggtgaacg cggtctaact ggagctcaag gtgaaaaagg agaccgaggc
     1861 gaacgcggtc taactggagc tcaaggagct aaaggtgaaa aaggagccca aggtgaacgc
     1921 ggtctaaccg gagcacaagg tgctaaaggt gaaaaagggg accaaggtga acgcggtcta
     1981 accggagcac aaggtgccaa aggtgaaaaa ggggaccaag gtgaacgcgg tctaaccgga
     2041 gcacaaggtg ctaaaggtga aaaaggagcc caaggtgaac gcggtctaac tggaactcaa
     2101 ggagctaaag gtgaaaaagg agaccgaggc gaacgcggtc tgactggagc ccaaggagct
     2161 aaaggtgaaa aaggagcccg aggcgaacgc ggtctgactg gagcccaagg tgctaaaggt
     2221 gaaaaaggag cccaaggtga acgcggtcta accggagccc aaggtgctaa aggtgaaaaa
     2281 ggggaccaag gcgaacgcgg tctaactgga gctcaaggtg aaaaaggaga ccgaggcgaa
     2341 cgcggtttaa ctggagctaa aggtgaaaaa ggggaccaag gtgaacgcgg tctcactgga
     2401 gcccaaggtg aaaaaggaga ccgaggcgaa cgtggtttaa ctggagctaa aggtgaaaaa
     2461 ggagcccaag gtcaggcagg tcgtgacggt gtaactccaa ccgtaaccgt taaagataat
     2521 aaaaatgacg gcactcatac tatcactatt aacgacggta gaggtaatgt tacaagtact
     2581 gttgtaagag atggtttcga tggcgcaagt ccattagttg cgactcaacg aaatgacgca
     2641 gataagacaa caactgttat cttctattac gataaaaatg gaaacaatga attagatgct
     2701 tctgataaga aattaaaaga agttgtaatt gcagatggtg ctaaaggtga aaaaggggac
     2761 aaaggtgaac aaggtcttca agggcgtgat ggtgaacaag gaccaaaagg agaagatggg
     2821 aaaaccccaa cagttaaagt aactgatggt caagatggaa cgcatacaat tacaattaac
     2881 gatggtaaag gtggtataac tactacagta gtaagagatg gatttgatgg tgcaagccct
     2941 cttgtttcta ctcatagaaa tgaagcagat aaaacaacaa ctgttatttt ctattatgat
     3001 ctaaatgata ataatcaatt tgatgaagga gatacaaaac ttaaagaagt tgttatcgca
     3061 gatggaaaac aaggaccaaa aggtgacaaa ggtgataacg gaaaagatgg attcacacca
     3121 gaagtaacag ttacagataa caataacgga acacacacca tcacaatcac acaaccagac
     3181 aacagaccat cattaacaac aatcgttaaa aacggtgaag atggaaaaac accaaaagtc
     3241 aaagcagaac gagatgatgc gaagaaacaa acaacattaa cattctacat tgacaaagat
     3301 ggagatggaa gttacacagc aggaaaagac gagttagttc aaacaacagt agttaaagac
     3361 ggacaagacg gagctgcagg agcatctgga cgtgatggaa aagaagtatt aaacggaaaa
     3421 gtagacccaa caacagaagg taaagatgga gacacattcg taaatacaca aacaggagat
     3481 gtattcgtta agaaaggtaa cacttgggaa ccagcaggaa acatcaaagg accgaaaggt
     3541 gacaaaggtg cagatggtgc caagggtgaa aaaggagccc aaggagaacg cggcctgact
     3601 ggagcgcaag gtgtcaaggg tgaaaaggga gatcagggtg aacgcggtct aactggatct
     3661 aaaggtgaaa aaggagccca aggtgaacgc ggtttaactg gagcgcaagg tgccaaaggt
     3721 gacaaaggtg aacaaggtct tcaaggtcgt gatggagctc aaggaccaaa aggcgcagat
     3781 ggacaaagag gaccagcggg accacaagga ccaaaaggag aacaaggtaa tccaggaact
     3841 ccaggtaaag atggaaaatc tctaattgct gttaaaaatg gaactgaaac aaaagtctat
     3901 gtagaggatc cggctagacc aggacaacca cttaatccaa accaaccatt agcaactatc
     3961 actgatggaa gaaacggtac taatggacaa tcaccaacaa ttacagcgac acgaagtgtg
     4021 caaaatggca aaaatggtgt attagtaaca attacaccag taggaggtcg tccacaaaca
     4081 acatttgtag aggatggaca aaagggtgct gatgggaaaa ccccaacagt aacaataact
     4141 gaggggcaaa acggcacaca tacattaacg gttcataatc caggaagtcc agatgtgaca
     4201 actacgatcc gtgatggagc tacaggacaa gcaggtcgtg atggtaaaga tgtattaaac
     4261 ggaaaagtaa atccacaacc aaaccaaggt aaaaatggag ataaatatat taataccgaa
     4321 accggtgatg tctatgttaa aaacaatgga aactgggata aagaaggcaa catcaaaggc
     4381 cctaaagggg acaaaggtgc agatggtgct aaaggcgaaa aaggagacca aggcgaacgc
     4441 ggcctaactg gagcgcaagg agctaaaggt gcggatggcg cagtaggtcg tgatggacgt
     4501 gacggtaaag acgtgttgaa cggcaaagct aacccagaag cacatcaagg taaagacggc
     4561 gataaatacg ttaatacaga aacaggcgac gtcttcgtta agaataacgg caactgggat
     4621 aaagagggca acatcaaagg ccctaagggt gacaaaggtg aacgcggaga agatggtaag
     4681 actccagaag taactgtaac tccaggtaaa gatggccata gtactgacat tacattcact
     4741 gttccaggta aagatccagt tacagtaaat gttaaggacg gagaaaatgg tctgaacggt
     4801 aaaactccaa aagttgattt acttcgtgtc caaggaaaaa acggaaatcc atctcataca
     4861 attgtgacat tctatacaga tgaaaacaat gacggcaaat atacaccagg aactgatgaa
     4921 cttctaggtt cagaaatgat taaagatggt gctaaaggcg cggacggacg agatggtaaa
     4981 tcattgctta ctgtcaagga tggtaaagaa actaaagttt accaagaaga tccagctaac
     5041 ccaggacaac cattaaatcc agaaaaacca cttgcggtaa ttagagatgg agtagatgga
     5101 aaatcaccta cagttacagc tgttcgtaaa gatgaagcag ggcataaagg tgtagaaatc
     5161 actgttgata accatgatgg ttcacaacca actacagtct ttgttcaaga tggtgctaag
     5221 ggagaaactg gtgcaaccgg tcaggatgga caaactccta caatcactac tcaacgtgga
     5281 caagatggcc aaagcactgt tgtaactatc acaacatcag gtaaagatcc agtaaccttc
     5341 actgtaaaag atggtaagaa tggtaaagat ggccgtgcac cgaaaatcaa agtagaagat
     5401 attacttcac cttcaagaat tagacgcgat acagatgctg ctgcaactcc aacgcgtaac
     5461 ggtatccgtg ttacagttta tgatgatgtt aatgacaatg gggtatacga cgaaggtgtc
     5521 gataaagtat taaatagtaa agatatttat aacggtatag atggacgtga tggttcagct
     5581 ccaactatta ctacaaaaga taatggagat ggaactcaca ctatcacagt tcaaaaccca
     5641 gatggttctg aatcaacaac agttgttaaa gatggtaaag acggtaaaac tgcgaatatc
     5701 actacaacag aaaacccaga tggaagccac acaattacgg tgacaaatcc agatggttca
     5761 actaaagaaa ctgttgttaa aaacggtaaa gacggtaaga ctcctaaagt tgaagtaacg
     5821 gataacaacg atggaactca tactgttaaa gttacagatg gagacggcaa tgttaccaac
     5881 gctatcatca aagatggtaa agacggtaaa gctgcaacag caacaactac tgaaaatcca
     5941 gatggaagcc acacagtaac aatcactaac ccagacggaa ctaagaatga gtttgttgtt
     6001 aagaatggac gtgacggtgt tgacggacgt actccaaccg catctgttcg tgataatgga
     6061 gacggaagtc atacaatcgt tattacaaat ccagaaggtg tgacaactga aaccacagtt
     6121 cgtgatggta aatcaccaaa agtgactata actgatgaac aaaatggaac tcataagatc
     6181 tctgttctaa atggtgacgg aacaactaca acaactacag ttcgtgatgg tagagaacca
     6241 aaacttgaag ttattgataa caacgatggt tcacacacta ttaaagtgac aggtgctgat
     6301 ggtaaaggaa cgacaactac aatctttgat ggtaaatcac caaaagcgaa catcgttgat
     6361 aacggagatg gaactcatac attaacaatc gtagattctg atggtcgtga atacaaatct
     6421 attatcaaag atggtaaaga cggcaaagat agcgtttcac caactgtaac tgttaaaaat
     6481 aataacgatg gaactcacgt tgttacaatc actaatccag atggaagtaa gacagaaatg
     6541 gtgattaaag acggtaaaga tggtaaatca ccaaaagttt ctgttgaaga taatggtgat
     6601 ggtagtcata caatcacaat catcaattct gatggaactg tgacaaaaac agttattaaa
     6661 gatggtagag atggacgtga tggtcgagac ggcaaagacg gtaaagacgg taaagatgga
     6721 aaatgtggat gccaagacaa accagtaaca ccatcaaatg acaaaccagt tcctccaaca
     6781 ccaaatgtgc cgacaccaga agtaccggtt aaacctgtgc cagcggttcc agaacaacca
     6841 gtagtaccaa caccggctca accagcaact ccagtaaatg ctaacccagt agcaccaact
     6901 acaggtaaag aaaaccgtgg ggacaaatta cctgaaactg gaagccaatc tgattatatc
     6961 tctgttcttt taggtagcgg tattctattg agcctatatg taggacgaag aaaagaagct
     7021 taatcagcca aaagcagtca ctaatttctc gaaagaagat gataagtgat agatctcgaa
     7081 tgaaagaata ttcttagaat gattttccta tgatggaaaa gactgctaaa aaccttgagt
     7141 ggtaacgctc aaggtttttt tggatggctg atgtttgtct gaaaatcttg attgagaaat
     7201 aaaaagccac cctattaggt ggctataatt gatttagact ttctaaaatt ttctattttt
     7261 tactgaatcg cttcgaccaa gtcttgagct tgtttttcaa gtgagtttag gactgtttct
     7321 tcaagaacca attttccgtc tgcccaggca gagtcattaa cacgtgcagc agtgaaatca
     7381 ccaacgcctt gtgtacggat aaatggcaag aggtctttgt agatagcgaa aagttgatcg
     7441 tgccctgcat tggctacaga tgatactgtg acaaacttgt cttgaagggc agaaacgcca
     7501 cgtgtatcag acaagtcaag ggcacgagat agccagtcaa gcaagttttt cactgtacca
     7561 gggatagaga agttgtagac tggagagaaa atccagatag catccgcaac gagaactgct
     7621 tcacgagcag cagctacagc tggatgtgtt ggaacttcca aatcttggct gaagagagga
     7681 agggctgaat aatcaaggta gctaacttcc gctttaccag caagtgcttt ctcagcttcg
     7741 agcgccattt ggtggttgaa agaaccttga cgtagtgatc cgacgataaa taatactttt
     7801 ttagacatga tgtgtctcct ttttcttaaa atttcgagaa ctctctcgtt gttataaaat
     7861 atattacaac aaaacaaaag aacttgcaag aaatttgctc agagtttatt tgaaaattgt
     7921 aaaatttcgg aaactctaat catttgtgac tgaaaaagta agaactgttt cactttcaaa
     7981 atcggttttg tttgaaggga gaaatagaat tggttgctca aagcacagct ttgaggttgc
     8041 agataaagct gacgtggttt gaagagattt tcgaagagta ttcttatatt cacttgtaat
     8101 aaagcttaat agatggtaaa atagacgagt gaaaaaaaga caaaacaaag caaaaaataa
     8161 tctactgtgg cagtatggtc tagggatgac gattttgttt gtggttatca gtggttcctt
     8221 tctgtatatg gtttctctta gcatgaaacc ctatcaaaca gctaaaagtg aaggagaaaa
     8281 attagctcag cagtatgcag gattagagca ggctgatcag gttgatttat acaatggctt
     8341 ggaatcttat tacagcgttc ttggtcgtaa caaacagcaa gaagcgcttg ctgtcctgat
     8401 tggtaaagat gaccataaga tttacgttta tcagctaaat cagggtgttt cacaagaaaa
     8461 agcagaaacg gtttctaagg aaaagggagc tggcgagatt gacaagataa cctttggccg
     8521 ttatcaagat aagccaatct gggaagttaa gtcaggatct gatttttatc tagtagattt
     8581 tgaaacagga gcattggtca acaaggaggg cctatgaaac tatccaaccg tgttttagaa
     8641 atggaagaaa gtgtgacttt ggctgctgga gcccgtgcca aagcactgaa ggctgagggt
     8701 agagatattt tgtctctaac cttgggtgag ccagatttca ctactcccaa aaatatccaa
     8761 gatgccgcca ttgcatcgat tcgagatggg cgtgcttctt tttatacagt aacctcaggt
     8821 ctgccagagc ttaaggcggc ggtcaatagc tactttgagc gcttttacgg ctattctgta
     8881 gcgtcaaatc aagtgacagt cgctgcggga gccaaatatt ctctctatac cttctttatg
     8941 gctgtggtca atccaggtga tgaagtcatc atcccaaccc catactgggt tagctatgga
     9001 gatcaggtca agatggcaga aggcgttccc gtctttgttt ctgctaagga agacaatcac
     9061 tttaaggtga ccgtagagca gttagaagca gctcgcactg acaagaccaa ggttttggtg
     9121 ctgaattcgc catctaatcc cacaggtatg atttacaccc gtgaggaact cttggcaatt
     9181 ggaaactggg ctgtagaaaa tgatattctc atcctagcag acgatatcta tggccgcttg
     9241 gtttataatg gtcatgagtt cacaccgatt tctagcctat cggaagcgat tcgcaagcaa
     9301 acagtggtca tcaatggtgt gtctaaaact tatgccatga ctggttggcg gattggttat
     9361 gccgttggag aagcagacat tatcgctgcc atgtccaaga ttgcaggtca aacaacttcg
     9421 aatccgtcag cagtagccca atatgcagca gttgaggctc tatcaggtga gcaagatact
     9481 gtagaaagca tgcgtcaggc ctttgaggaa cgtcttaata ccatctatcc cctccttgca
     9541 gaggtaccag gatttgaagt ggtcaaaccg caaggggcct tctatctctt cccaaatgtc
     9601 aaaaaggcca tggagatgaa aggctacacg gatgtgacag actttacaac tgttatctta
     9661 gaagaagccg aagtggcctt ggtaacagga gctggatttg gagcaccaga aaatgtccgc
     9721 ctcagctatg cgacagacct agacacgctt aaagaagcag tcgaacgctt gaaagcattt
     9781 atgggtagtg agaatgattg atcattttga gattaaggta aaggatttac aaatttcaga
     9841 aggattttat aggagttttc tcgctccttt ggactataaa ttggctttta agactagttc
     9901 tctaattagt tttctatccc cgaacagccc tcatcctggt ggtgattttt ggctgactca
     9961 gggaacacaa gatcctgttc attttgcttt tttagcagaa aataaggaag aagttcaggc
    10021 ttgttatgag gctggcttag aggcaggtgg gcgagacaat ggggctcctg gttatcgaag
    10081 tgagcatccg atttactatg ctgcttttat gattgacctg gatgggaaca atatagaggt
    10141 ggtttgccat aaagaataaa aaagaaaagg aaaaataatg acaaaacgtg taacgattat
    10201 tgacgtaaaa gactatgttg gtcaggaagt gacgattggc gcttgggttg ccaacaaatc
    10261 aggaaaagga aaaatcgctt tcttacaatt gcgtgatgga acagccttct ttcaaggtgt
    10321 ggcttttaaa ccaaactttg tcgaaaaatt tggtgaagaa gtgggacttg agaagtttga
    10381 tgttatcaaa cgcttgagcc aagaaacgtc tgtttatgtg acaggtattg tcaaagagga
    10441 cgaacgttct aaatttggct atgagttgga catcacagac atcgaagtga tcggtgaatc
    10501 tcaagactac ccaatcacac caaaagaaca cggaacagac tttttgatgg ataaccgtca
    10561 cttgtggcta cgctctcgta agcaagtagc tgtgttgcaa atccgtaacg ctattatcta
    10621 tgcaacttat gagttctttg acaagaacgg ttttatgaag tttgacagcc caattctttc
    10681 aggaaatgcg gcagaagatt ctacagaact ctttgaaact gactacttcg gaacgccagc
    10741 ctacttgagc caatcaggtc agctttacct agaagcaggg gctatggctc ttggtcgtgt
    10801 ctttgacttt ggtccagttt tccgtgctga aaaatcaaaa acacgccgtc acttgactga
    10861 gttctggatg atggatgctg agtactcata cttgacacat gatgagtcgc ttgacttgca
    10921 agaagcttat gtgaaagctc ttctacaagg cgttcttgac cgcgcgcctc aagccttgga
    10981 aaccttggaa cgtgatacag aactcttgaa acgctacatt gcagagccat tcaaacgtat
    11041 cacttacgat caagccattg acctcttgca agagcatgaa aatgatgaag atgctgacta
    11101 cgagcatctt gagcatggtg atgactttgg gtcaccacac gaaacttgga tttcaaacca
    11161 ctttggtgtg ccaacatttg tcatgaacta tccagcagcc atcaaggcct tctacatgaa
    11221 accagttcct ggaaatccag agcgcgtgct ttgtgcagac ttgcttgctc cagaaggcta
    11281 tggagaaatt atcggtgggt ctatgcgtga ggaagattac gatgcccttg tcgctaagat
    11341 ggatgaactt ggcatggatc gtacagaata tgaattctac cttgaccttc gtaaatacgg
    11401 tacagttcca cacggaggat ttggtatcgg tatcgaacgt atggtaacct tcgcagcagg
    11461 aacaaaacat atccgtgaag ctattccatt cccacgtatg ttgcaccgta tcaaaccata
    11521 agaacaggaa aacgcccatg tggcgttttt tcgaattcta gtgtttttac ttgcgtaaaa
    11581 aaattttttc tagtataata gtttattgtg agcgaacctc acttacccct tgcaaagtct
    11641 tggggtcatt agaccaaaag gaggaacata tcaatggcta aatacgaaat tctttatatc
    11701 attcgtccaa acattgaaga agaagctaaa aacgctttgg tagcacgttt tgactctatt
    11761 ttgactgaca acggtgcaac tgttgttgaa tcaaaaactt gggaaaaacg tcgtcttgca
    11821 tacgaaatcc aagatttccg tgaaggactt taccacatcg ttaacgttga agcaaatgac
    11881 gatgcagctc ttaaagagtt tgaccgtctt tcaaaaatca acgctgacat tcttcgtcac
    11941 atgatcgtca aaattgacgc gtaagaaggt aaactatgat taacaatgtt gtacttgtag
    12001 ggcgtatgac acgtgacgct gagttgcgtt ataccccatc aaatgtagca gttgcgactt
    12061 ttactcttgc agtaaaccgt acatttaaga gtcaaaatgg tgaacgtgag gctgatttta
    12121 tcaatgtcgt tatgtggcgc caacaggctg aaaatcttgc taactgggct aaaaaaggct
    12181 cacttatcgg ggtgacaggt cgtatccaga ctcgtagtta cgataaccag caaggacaac
    12241 gtgtctacgt gacagaggtc gtggctgaga atttccaaat gttggaaagc cgtagtgtgc
    12301 gtgagggtca cacaggtgga gcttactctg caccaactgc aaactattca gcacctacaa
    12361 attcagtacc agacttttca cgtaatgaaa atccatttgg agcaacaaac ccattggata
    12421 tttcagatga tgatttacca ttctaatgga caactaaact tataaaggag aaaaaacatg
    12481 gctcaacaac gtcgtggcgg attcaaacgc cgtaaaaaag ttgattacat cgcagcaaac
    12541 aaaattgaat atgttgatta caaagatact gagcttctta gccgtttcgt ttcagaacgt
    12601 gggaaaatcc ttcctcgtcg tgtaacagga acttcagcta aaaaccaacg taaagtaaca
    12661 acagctatca aacgcgctcg cgtaatggct ttgatgcctt tcgtaaacga agattaaaga
    12721 aaagacccag ttgggtctct ttttcatgag cttgaaaaca agaaagcgaa ttaagacccc
    12781 aacgggtctt ttttcacaag ctttgaaatg agagagcgag ttaggctcag tggctccctt
    12841 tttacgagtt tgaaaacaag agaacacatt aaaagcacga tggagctttt tcataaatat
    12901 gtctcttttc tcatccaaga aattaaaata aaaatgataa actttttgaa aataggggat
    12961 ttgcctactt tatgatataa tggaaagagt tagattgaaa gaggtagtga gatggacgcg
    13021 aaattaagat acaaggcaaa gaagatcaag attgtctttt ttgatattga tgatacattg
    13081 cggaattcaa agacagggtt tattccaact acaattccca ctgtttttaa acagttgcgt
    13141 gaaaaaggaa ttttaacagg aatcgcctct ggacgtggca tttttggtgt tgttccagag
    13201 attcgtgatc tcaagcctga cttttttgta actttgaatg gggcttatat cgaagataaa
    13261 aaaggtcagg tcatttatca gcatcagatt gaaaagtcag atgttgagga gtatatctct
    13321 tgggctaagc aagaaggaat tgagtatggc ttggttggga gtcatgatgc caagttgtcg
    13381 actcgcaccg atatgatgag tgaagctatc aatccaattt atcccgactt agatgtagat
    13441 cccgatttcc atgaaaaaga agatatctat cagatgtgga cttttgaaga taagggagat
    13501 gacttgcact tgcctgacag tctctcagac aaacttcgca tggttcgttg gcatcaacat
    13561 tcgtctgata ttgtgccgat ttcaggctcc aaagcgacgg gggtggaaaa ggttgtggaa
    13621 caccttggct tgaaaccaga gaaggtcatg gtttttggag atggtctcaa cgacttggaa
    13681 ctctttgatt atgctggaat cagcgttgcc atgggaattt ctcatgataa aatcaaagaa
    13741 aaagcagatt atattacaaa aacattagaa gaagatggca tttttgctgc cttagaagta
    13801 tttggtatgg tagaaaaaga attgcatttt ccacaagtag acattgaaac agtagaaggt
    13861 cctcttgcga ctattaagac caatcacgga gacttacgta tcaagctttt ccctgaacat
    13921 gctcctaaaa cagtggctaa ctttgtatat ctttcaaaag atggctacta tgatggtgtc
    13981 attttccacc gtattatcaa ggactttatg atccaaggtg gagacccaac tggaactggt
    14041 atgggtggcg agtcaatcta cggcgaatct tttgaggatg aattctcaga agagctttac
    14101 aatatccgtg gtgctctttc catggcaaat gctggtccaa ataccaacgg cagccagttc
    14161 tttatcgtgc aaaaccaaca cctaccttat tctaagaaag aaattactcg tggtggttgg
    14221 ccagaaccga ttgcagaaat ctatgccaat caaggtggga cacctcacct agaccgccgt
    14281 cacacggttt ttggtcagtt agctgatgaa gcatcttacg ctgtcttgga tgccattgct
    14341 gctgttgaga caggagctat ggacaagcca gttgaagatg ttgtaattga aactattgaa
    14401 atcgaggact aagatgaaaa tcggtgataa gctaaagggg cgtattacag ggattcagcc
    14461 ctacggtgcc tttgttgagt tagagacggg tgatacgggg ctgattcaca tttcagagat
    14521 tcggacagga tttattgaaa atattcatga aaccttgaaa gtcgatgaag aggttcaagt
    14581 tcaggtagtg gatttagatg aatttacagg gaaagccagt ctttctatcc gcactttgga
    14641 ggaagaaaag taccagtttc ctagacggag acgcttttca agcgaccgct ttaactacgg
    14701 ctttgctccc tttcgacgaa tgctaccaat ctggactgga gaagccctgc accatttaaa
    14761 aaagaagaag tagtttagag ttttatattt tttgtaatgt taatataaat ttgctataat
    14821 aggaccatgg aagaagaaca attattaaaa tcaggagagc gcattaacca gctcttttcg
    14881 acagatatca aaatcattca aaatagagag gtttttagtt attcggtgga tagtgttctc
    14941 ttatcacgat ttccacgttt tcctaagaag gggttgattg tggatttttg tgctgggaat
    15001 ggagcagtgg ggctttttgc tagcactcgt actcaagcac agatattgtc tgttgagatt
    15061 caggagcgtt tggcggatat ggctgaacgc tctgtccgtt tgaatggatt agaggaacaa
    15121 atgcaggtca tctgcgatga tttgaaaaat atgcctgctc acattcaggg gagtaaggtg
    15181 gatatgattt tgtgtaatcc accctatttc aaggtggatc ctcattctaa tctgaacgag
    15241 agtgaacatt atctcttggc tcgacacgaa atcacgacta atttggaaga aatctgtcat
    15301 agtgcccaga gtattctcaa gtctaatggg cgtttggcca tggttcatcg tcctgatcga
    15361 cttctggata ttttggatac gttaaaacga cataatctag cccctaagcg cctgcagttt
    15421 gtttatccaa aaagagaaaa ggaagccaat atgcttttga ttgaggcgat caaggatggc
    15481 tcaacaagtg gctttaaggt cttacctcct ttgattgtcc acaatgatga tggctcttat
    15541 acgcccgaaa tcgaagagat ttattatgga tcataaggct tatatgtacg ttctggagtg
    15601 tcgtgatgga tcctactata taggctatac gactgatgtg agaagacgcc tcgctatcca
    15661 caatagtggg aagggagcca aatatacacg agcacgcttg ccagtcaaac ttatctatgc
    15721 tcaaggtttt gccagtaagg aagaagccat gtcggctgaa gctcttctca agcgtaagaa
    15781 gaggccacag aaggaagaat ttttatctga aaatcaagat agaaatttac tcagttattt
    15841 tgaagaatcg tggggagtcc tttgactcct cttacctttg tcaaaaagcg aaaaaaatgc
    15901 tacaataaag aaaataaaca aaatgaggta ttatcatgtc caagattcta gtatttggtc
    15961 accaaaatcc agactcagat gccatcggat catctgtagc ttttgcctac cttgcaaaag
    16021 aagcttacgg tttggatacg gaagctgttg cccttggaac tccaaatgaa gaaacagcct
    16081 ttgtcttgaa ctattttggt gtggaagcac cacgtgttat cacttctgcc aaagcagagg
    16141 gggcagagca agttatcttg actgaccaca atgaattaca acaatctgta tcagatatcg
    16201 ctgaagtaga agtttacggt gttgtagacc accaccgtgt ggctaacttt gaaactgcaa
    16261 gcccacttta catgcgtttg gagccagttg gatcagcgtc ttcaatcgtt taccgtatgt
    16321 tcaaagaaca tggtgtagct gtgcctaaag agattgcagg tttgatgctt tcaggtttga
    16381 tttcagatac ccttcttttg aaatcaccaa caacacaccc aacagataaa atcattgctc
    16441 ctgaattggc tgaattggct ggtgtgaact tggaagaata tggtttggca atgttgaaag
    16501 ctggtaccaa cttggctagc aaatctgctg aagaattgat tgacatcgat gctaagactt
    16561 ttgaactcaa cggaaataat gtccgtgttg cccaagtgaa cacagttgac atcgctgaag
    16621 ttttggaacg ccaagcagaa attgaagctg caatgcaagc tgccaacgaa tcaaacggct
    16681 actctgactt tgtcttgatg attacagata tcgtcaactc aaactcagaa atattggctc
    16741 ttggtgccaa tatggacaag gtcgaagcgg ctttcaattt caaacttgaa aacaatcatg
    16801 ccttccttgc tggtgccgtt tcacgtaaga aacaagtggt acctcaatta actgaaagct
    16861 ttaatgcgta agattttggg tgtcagctca aaatcggaaa gtctagtttg ccttatatcg
    16921 aaaggagttt cggctccttt ttttctagga gtgaagtatg ttagaaaatg gcgatttgat
    16981 ttttgtgaga gatgggtcag acatgggaca ggccatccag acttccacag gtaactatag
    17041 ccatgttgcc atttatttgg atgggatgat ttatcatgct agtggacagg ctggtgttgt
    17101 ctgtcaagaa ccggcagact tctttgagtc caatcatttg tacgacctct atgtttaccc
    17161 agaaatggat atccagtcgg tgaaggaaag agcttgcaga catcttggag caccctacaa
    17221 tgcttctttc tatccagatg cagctggttt ctactgctcc cagtatatag cagaaatcct
    17281 acctattttt gaaactattc ctatgaaatt tggagatggg gaacaggaga ttagtgattt
    17341 ttggagggag tattacatag aactaggtct gcctgttcct ctgaaccaag ctgggaccaa
    17401 tcctagtcag ttggcagcat cgcctctgtt acaatgtaaa gaaaggaatc ttcatgattc
    17461 agatttttaa tccatctcgt ttgacgagac agccattttt ggagaattga tccgctatct
    17521 ggaccagtat gaggatgtga ttctacgtga aaataagcgc tattacctca attttcctac
    17581 gcttgaatca cttgatagtc ttgaactgga tcaagagatt tttgtcagag aagctagtcc
    17641 ggtctatcaa gccttgttgg agtagagttt tgagacggaa ttgcgcaatc aaatcaatgc
    17701 agctatttta gttgaaagac ggactttgcg cgcattaaaa tgaccctgtc caattatttt
    17761 tacaaggtca aacagcagta tcctttgaca gaaaaacagc aggagctcta tgacatttta
    17821 ggagatgtta atcctgagta tgcactcaag tatatgacgg cttttttgtt gaaatttctc
    17881 aaaaaagacc agcttatgca gaaatgccgt gatatctttg tggacagttt ggttgtctta
    17941 ggctatattg tgcaaaatga agatagaaag tatgagttgg ctatcgattt tgataaggag
    18001 agattaactt tctacttagc gtgatttctt gtttctgagc acattgtttg actttcctta
    18061 gtattcggta taaactatat gtaaccggta acacatatcg gaataaacta aaggagacaa
    18121 tcatatgtca cttgaaaaca aattggaaca agcaacaggc gctgtcaaag aaggttttgg
    18181 taaagttact ggagacagca agacagaact tgaaggagct gttgaaaaaa cagttgctaa
    18241 ggcaaaagac gttgtagaag acgcaaaagg tgctgtagaa ggtgccgttg aaggtttgaa
    18301 aaacgttttt actaaataat aggaaaaaat caagggtttc attttccctt gattttttct
    18361 attcttataa ataattttct gcgacggctg tatctcctgg gtaggattct ttcttgccct
    18421 ggatgatttg gtaacaatcg gctcccttac ccgcaataat aactgcatct aattcgtgat
    18481 ttgtgatagc cattgccgcc ttgatggctt cttggcgatc cgcaatcttt tcaacaggat
    18541 gattgatgta gctactaatt tcatctgcaa tggccattgg gtcttcatag ttagggtcat
    18601 cagcagtcag aaagacttga atctcagggt gttgattgag gaggaggcca aagtccttac
    18661 gacgactttc tcccttgttt cctgttgttc ccagaaccag agcaatcttt ccggtttgat
    18721 gagtttcaac cacattgatg agttttttca gactatcccc attgtgggca tagtcgatga
    18781 agaccttggc tccatttttc tgagtgagga cttccatacg accaggaacg cgggttgcag
    18841 cgatgccttt tttgatgtcc tcaagacttg ctccgagacg gagacaagca agtccagcag
    18901 caactgcatt ttcttggttg aagttgccaa tgagttggat atcataatct ccagcgagtt
    18961 tacccgtagc tgaaaagcta aaggctttgg aattctcgat ttggttatca aattggctac
    19021 catagaaatc atggtcttga tcttcaacct gttctttcaa gactgagaag tggtccatgt
    19081 cactgttaat gatgactgct cggctatttt ccatcaagag acgcttgtgg tagaaatagt
    19141 cttcaaagct agggtgttca atcgggccga tatggtcagg agtgatgtta agaaagactc
    19201 ctacatcaaa ggtcagtcca tagactcgat ggactagata ggcttgactg gagacttcca
    19261 tgatgaggtg ggtacggtca ttttgcacag cctgattcat catgtcaaag aggtcaatac
    19321 tctcaggggt tgtcaacgct gacttaaaga aagtctcgcc atcaagagtt gtgttcatgg
    19381 tcgacaacat agcaggtcta tgcccttgag ataagatgtt ataggcgaaa taggttgctg
    19441 ttgtcttacc cttagtacca gtaaaggcaa ggagtttgag tttctcttgt ggattaccat
    19501 agaactccat ggcaatcaaa ctcatggctt tctttatatc gttcacaatg atgacaggga
    19561 taccgacttc gtagtccttt tcagctacat accaagctaa accttgtgtt atagcagaaa
    19621 gaaggtattc ttttttaaag gcagcgcctt ttgcaaaaaa aagagtgtct tctgttactt
    19681 ttcggctgtc gtagctgatg ctatcaaaaa taactttgct gtagttgtag tggtaatgac
    19741 cttggtcaat aatttcgcga aaaaggccat ctttctttaa aatatctaat acggtttcaa
    19801 tcttaatcat actttctatt gtaaaccgaa agtcgtaaat ttacaagtaa caaggaaaag
    19861 tttataatgg aagataagga gtttttccta gttatcaaaa ttgaatgagg aatctatgtc
    19921 gcacgaaaac aatcaccagc aggcccagat gttacggggg actgcttggc taacggctag
    19981 taactttatc agtcgcctac tcggggctgt ttacattatc ccttggtaca tctggatggg
    20041 ggcttatgca gctaaggcaa atggtctctt taccatgggt tacaatatct atgcttggtt
    20101 cttgttggtt tcaacagcgg ggattccagt tgcggtggcc aagcaagttg ccaagtataa
    20161 taccatgcga gaagaagagc atagctttgc cctgattcgg agcttcttag gctttatgac
    20221 aggactaggc ctggtttttg ctttagtctt gtatgtcttt gctccttggc tagcagactt
    20281 gtctggcgtg ggcaaagact tgatcccaat catgcaaagc ttggcttggg gagtcttgat
    20341 tttcccgtct atgagtgtta tccgaggatt tttccaaggg atgaataacc tcaaacccta
    20401 tgccatgagc caaattgctg agcaggtcat tcgtgttatc tggatgctcc tagcaacctt
    20461 tatcattatg aagctcggtt caggagatta tctagcagcc gttacccaat caacctttgc
    20521 tgcctttgtc ggtatggtag ccagttttgc agtcttgatt tatttccttg cccaagaagg
    20581 ttcactcaaa agagtctttg aaacaggaga taagattaac agtaagcgtc tcttggttga
    20641 taccattaag gaagccattc cttttatcct gacagggtct gccatccagc tcttccagat
    20701 tttggatcag ctgaccttta tcaatagtat gagctggttt accaactaca gcaatgagga
    20761 cttggttgtc atgttttctt atttctcagc caatcctaat aaaatcacga tgattttgat
    20821 ttctgtaggg gtttcgattg ggagtgttgg tttgccactt ttgacggaaa actatgtcaa
    20881 gggggacttg aaagcagctt ctcgtctggt tcaggacagt ctcaccctac tctttatgtt
    20941 cttgctacca gcaacggttg gagtggttat ggtaggagaa cctctttata cggtcttcta
    21001 tggtaagcca gatagtttgg ctatgggctt atttgtcttt gcagttttgc agtctattat
    21061 tttaggtttg tacatggtct tgtctccaat gcttcaggcc atgttccgca accgcaaggc
    21121 cgttctctat tttatctatg gttctattgc caagctagtc ttgcaactac ctaccatcgc
    21181 cctcttccac agttatggtc ctttgatttc aacaaccatt gctctcatca ttcctaacgt
    21241 cttgatgtat cgggatattt gtaaagtaac tggtgtcaag cgcaaggtga ttttgaagcg
    21301 aaccatttta atcagtttgc tgaccctagt catgtttctg ttaataggaa ccatccagtg
    21361 gctgttagga tttttcttcc aaccaagtgg acgtttgtgg agcttctttt atgtagctct
    21421 tgtcggtgcc atggggggtg gactttatat ggttatgagt ctgcgtacct atttattaga
    21481 taaggtaata ggaaaagtcc aagcagatcg cctgcgagca aaatttaagc tttcgtaagg
    21541 tggcatttat aaataaaagg aataagttga acaatctttt aggagaactt gaaacttatt
    21601 ccttttttta gcgtcaaaat agctaaaatt atctttcaaa aatagaaaat gaggtttttt
    21661 tcgatttttt ggaaaaaaca attgactaaa caaacaaaga gttgtataat aaaacaaata
    21721 tttaaagcag gaggttctgg aaatgaaaaa gtctaagagc aaatatctaa ccttggcagg
    21781 tcttgtcctg ggtacaggag ttttattgag cgcgtgtgga aattctagca ctgcatcaaa
    21841 aacctataac tatgtttatt caagtgatcc atctagtttg aactatctag cagaaaaccg
    21901 cgcaacaacc aatgatattg tgaccaatct ggtagatggt ctcatggaaa atgaccaata
    21961 tgggaactat gtgccatcct tggcagaaga ttggactgtt tctcaggatg gtttgaccta
    22021 tacctacaaa cttcgtaagg atgccaagtg gtttacttct gagggagaag aatatgcacc
    22081 tgtaactgcc caggattttg tgacaggatt gcaatatgca gctgataaaa aatcagaagc
    22141 cttgtatcta gtgcaggact ctgttgcagg tttggatgac tatatcactg gtaaaacaag
    22201 cgacttttca actgtcggta tcaaggctct tgatgatcaa accgttcaat acaccttggc
    22261 tcgaccagaa ccttactgga actcaaaaac aacctcaacc attcttttcc cagtcaatgc
    22321 agatttcttg aaatcaaaag gggatgattt tggcaaggta gaccctgcta atattttgta
    22381 cagtggacct ttcttgatga aagcttttgt ttcaaaatct gtcatcgaat acaagaaaaa
    22441 tcctaactac tgggatgcta aaaatgtctt tgtagacgat gtgaaattga cctactatga
    22501 tggtagcgac caagaatcac tggaacgtaa ttttacagct ggtgcttata ctacggctcg
    22561 tctttttcct aacagctcca gctatgaagg gattaaagaa aaatacaaaa acaatatcat
    22621 ctatagtatg caaaattcaa cttcatattt ctttaatttt aacctagata ggaagtctta
    22681 caattatact tctaaaacaa gtgacattga aaagaaatcg actcaggaag cagttctcaa
    22741 taaaaatttc cgtcaggcta tcaattttgc ttttgacaga acatcttatg gggctcagtc
    22801 tgaagggaaa gaaggtgcaa caaagatttt gcgtaaccta gtggttcctc caaactttgt
    22861 cagtatcaag ggaaaagact ttggtgaagt tgtagcctct aagatggtca actatggtaa
    22921 ggaatggcaa ggtatcaact ttgcggatgg tcaagaccct tactacaatc ctgagaaagc
    22981 caaggctaag tttgcggaag ctaagaaaga actcgaagca aagggtgttc aattcccaat
    23041 ccacttggat aagactgtgg aagtaacaga taaagtaggc atacaaggag ttagttctat
    23101 caaacaatca attgaatctg ttttaggttc tgataatgta gtgattgaca ttcagcaatt
    23161 aacatcagat gagtttgaca gttcaggcta ctttgctcaa acagctgctc agaaagacta
    23221 tgatttatat catggcggtt ggggacctga ttatcaagac ccgtcaacct atctcgatat
    23281 ttttaatact aatagtggag gagttctaca aaatcttgga ctagagcctg gagaagccaa
    23341 tgacaaggct aaggcagttg gactggatgt ctatactaaa atgttggaag aagctaataa
    23401 agagcaagat ccagccaaac gttatgagaa atatgctgat attcaagctt ggttgattga
    23461 tagttcttta gttcttccaa gtgtttcgcg tgggggaaca ccatcattga gaagaaccgt
    23521 accatttgct gctgcctatg gtttaaccgg tacaaaaggg gttgaatcat ataaatacct
    23581 caaagtacaa gataagattg tcacaacaga cgaatatgca aaagccagag aaaaatggtt
    23641 gaaagaaaaa gaagaatcca ataaaaaagc ccaagaagaa ttggcaaaac atgtcaaata
    23701 aaatctaatt gtagataagt ttgtgtaaga agtctttgtg cttaaaaacg cataatatca
    23761 ggtatataaa atcctagatg ttatgcgttt tgttatagga agatttatta gattttatcc
    23821 tcaaattgag ttattcccga ctttatccgt tgaaatatta taaggagtat ttatgaaatt
    23881 aaaattatta agagtagata ctaaggtgat tatggggagt ttcttacttg ttctgtctag
    23941 tctacttgct ttgttgcttc cccttatctt aaagggttta atagatggga gttctattga
    24001 aaatataggt tccaaagtat ttcaatcgtt tttgattttt attggtcaag ccttgttttc
    24061 ttctattggt tactatctgt ttagtcaatc gggtgaaaaa aagatagcaa aaatcaggaa
    24121 aaaagtgaca gaggggttga tttatgcaga gaaatccttc tttgataaga gccaaagtgg
    24181 ggagttgact tctgccattg tcaatgacac gagtgtcatt cgtgagtttt taattacgac
    24241 tttcccaaat attattctga gtttagttat ggtacttggt tccattgtag tcttatttag
    24301 tcttgattgg aatctttctc tacttttatt catcactctt ccttgtatga tgtttattat
    24361 cttgcccctt tccaatatca gtgaaaagta tagtcgtcgt ttacaggagg aaatcggatt
    24421 tttaacaggg caattgactg aaaagattca agaacatgaa ttaattaaga ctaaccaagc
    24481 agagaaaagt gtacaggatg ttttggataa ttgtattgaa agggtacaaa acaattcact
    24541 gaaatcagat agggttactt cttttgagac cccgtttgct ctcttattta tctttgcgac
    24601 tatagctgtg atgctaacct atgggggtta tcgggtcagc gcaggatata tatctgtggg
    24661 aaccttggtt tcgtttttga tttacctctt tcaattactt aatcctatta gtaatatagc
    24721 taattttgta actgtttatt ctaggagcaa gggatcttca gttgcactgg agaacttgct
    24781 tgcagttcct aaagaaaaat ttgagggagg aaaatcggta tcaggacaag ggttgaattt
    24841 taaccatgtc tattttggtt atgatgaaaa tcgacctgtc ttaaaggata ttacttgttc
    24901 aattttcaag gggcaaaaaa ttgcttttgt tggaccatct ggatcaggaa aatcaacgat
    24961 tgtgcgtttg ttagagcagt tttataaacc gctttcagga gatattctaa tggagcaatc
    25021 aagtatatat gattttaact taaaagaatg gagaagtaaa atcgcttggg tttcacaaaa
    25081 taatgcagtc ttatctggca gtattcgtga caatctttgt ctcggtttga atcgcttagt
    25141 aactgatgat gaattgatga aagtgctaga cttagtatca ctaggtgatg agattcgctc
    25201 catgaaagag ggactagata ctgaagttgg tgaacgcgga cgactcttgt caggggggcg
    25261 aagccaaaga cttcaaatag ctagagccta cttaaaagat gctgaaattc ttatatttga
    25321 tgaagccact gctaatcttg atgcggattc tgagtatgcg attatcagta gcctctattc
    25381 tgtactaaag gagaagacgg ttgtgattat agcgcatcgt ttgtcaacgg taaaagatgt
    25441 ggattgtatt ttcttcttag aggaggggaa aatcactggc tcaggaactc ataaggaact
    25501 actggaaaat catgagcgtt atgctcgttt tgtgcaggag caaatgatag agtgaaggtg
    25561 tcttttgaga ttcaccattt tatagtctat taaagggagc aggaaaaact ccctttttat
    25621 atagtttgaa actataacta gctcttgaaa agaagaaaat gagttgatga aaataagtgg
    25681 tacaatagtt actatagatt tggaggaatt gtatgagcaa ggaattacac attaacacaa
    25741 ttttagcaca agcagggatc aagtctgatg aggcaacagg tgctttggtg acaccacttc
    25801 atttttcaac gacctatcag catccagagt ttggtcgatc tactgggttt gactatacgc
    25861 gcactaaaaa tccaactcgt agtaaggctg aggaagtctt ggcggctatt gagtcagcag
    25921 actatgccct agcgactagc tcagggatgt cagctattgt actggccttt agcgtctttc
    25981 cagtaggaag taaggtcttg gcagtgcgtg atctttacgg tggttctttt cgctggttta
    26041 accaagtgga gcaggaaggt catttccatt ttacctatgc caatacagaa gaagagttga
    26101 ttgccgagtt agaaaaggat gtggatgttc tctatatcga aaccccaacc aatcccttga
    26161 tgttggaatt tgatatcgaa aaactagcaa aattggctca tgctaagggt gccaaagtgg
    26221 tggtggacaa taccttctat agccctatct accaacgtcc gattgaagat ggagcagata
    26281 tcgttctcca ttcagcaacc aagtatctag caggccacaa tgatgtcttg gctggagtgg
    26341 ttgtgaccaa tagtttagaa ctatacgaga agctttttta caatcttaat acaacagggg
    26401 cagtcttgtc tccatttgac agctaccagt tgcttcgtgg tctcaagacc ttgtctcttc
    26461 gtatggagcg ctcaacagct aacgcccaag aagtggttgc ctttttgaag gattctccag
    26521 cagttaagga agttctctac actggtcgtg gaggcatgat ttcctttaaa gtagccgatg
    26581 aaacacgcat tcctcatatt ttgaacagtc tcaaggtctt ctcttttgcg gaaagtttgg
    26641 gtggagtgga aagtcttatt acttatccaa cgactcaaac tcatgctgat attccagcag
    26701 aagtacgcca ttcttatggt ttgacagatg acctcttgcg tttgtctatt gggattgagg
    26761 atgctagaga tttgattgca gatttgcgcc aagccttaga aggataagac aaagatggga
    26821 aaatatgatt ttacaagcct gcccaaccgt ttagggcacc atacctataa atggaaagaa
    26881 acagaaacgg atagtgaagt tctaccagct tggatagcgg atatggactt tgtagtcttg
    26941 cctgaaatcc accaagccgt gcaaacttac gcagaccaac tggtttatgg ttatacctat
    27001 gccagtgaag acttaattaa ggaagttcaa aagtgggaag ctacacaata cggttacaac
    27061 tttgacaaag aggctcttgt ctttatcgag ggtgtggtac cagccatctc aacagctatt
    27121 caaaccttta caaaagaagg cgaggcggtt ttaattaaca cgcctgtcta cccacccttt
    27181 gctcgcagtg tcaagttgaa taatcgtaga ttgattacta attccttagt ggaaaaggat
    27241 agtctgtttg agattgactt tgaccaactt gaaaaggatt tggtggaaga ggaggttaaa
    27301 ctctatgttc tttgcaaccc tcacaatcct ggtggacgtg tttgggaaaa agaagtgttg
    27361 gagaagattg gccaactctg ccaaaaacac ggtgttttgt tagtttcgga tgagattcac
    27421 caagatttga ccctctttgg tcacaaacac cagtctttca ataccatcaa tcctgccttc
    27481 aaaaattttg ctatcgtctt gagcagtgcc actaaaacat ttaatattgc tggaacaaaa
    27541 aattcctatg cagtcattga aaatcctaag ttgagactgg ctttccagaa acgcctgttg
    27601 gccaataatc agcatgaaat ttcaggcttg ggttatttgg cgacagaagc tgcctatcga
    27661 tacggtaaag attggctaga ggaactcaag caagtctttg aagaccacat caattatgtg
    27721 gtggatctat ttggaaaaga gactaaaatc aaggtcatga aaccgcaagg tacctacttg
    27781 atttggcttg acttttcagc ttatgacctg actgatgaaa cattgcaaga gttgttgaga
    27841 aatgaagcca aggttatcct caaccgtggt ttggattttg gagaggaagg aagtctccat
    27901 gcccgcatca atatagctat gcccaaatct ctgttgcaag aagtctgtca gcggattgtg
    27961 gctacttttg ccaaatgtta aaaatccagc cttctaggag aaaagtcttc ctagaaggct
    28021 attttcatag gcgaaaatat ggtataataa aaagataagg taaaggtgaa aatatggcta
    28081 aattgattcc ggggaaaatt cgtatcgaag gtgttgccct ttatgaaact ggtaaggttg
    28141 atatcatcaa ggaaaagaac aatcggctct acgctcgcgt tgcagaagaa gaactgcgct
    28201 atagtttaga ggatgatttg attttttgtg cctgtgattt ttttcaaaag aggggctact
    28261 gtgtgcattt ggcagcgcta gagcattttc tgaaaaatga tgagcgtggt caggaaatct
    28321 tgtggagtct ggaagaaggt catgaagaaa aagaggccgt tgaaaccaag gtgaccttgg
    28381 gtggcaagtt tttggatcga attttatctc caaaatcaga atgcgcctat gagttatcag
    28441 ctgtgggtca ggtggaagca ggaaccaatc atatcttgtg gactctgaga atcggccaaa
    28501 tcaatagcca aaaatactat gtcattcggg atattccact ttttttaagg attgtcgagc
    28561 agagaaagtc gtatatgatt ggcaagattt atgaggagtc tctttcttgg gaagcctttg
    28621 atgatgccag ccaagaactt ttgactttct tgcacggact aacggaggaa gggctggttc
    28681 cagacctctt tttccaaaac caaggtcggc acctcttttt ccctctgacc ttttttgagc
    28741 agggtgtgaa tttactgatg gccttgcccc attttcaatt tgaccatcaa gtggatagct
    28801 accaaaccct gctttttcag gatatgcatg ctgatgccaa tctatttgcc tttacagtaa
    28861 cagaatattc ggactatttt gaaatggaaa tcagtgagag tcctagggtc aatgtctttt
    28921 atcaaggagc agtacttttc cataaaggtc aggtttattt tttaacagac cagcagatgt
    28981 gtcttctcaa ggaaattaaa gcgttacctg tgggtcaaca aggaaagaaa tacctgcaat
    29041 ttgatagcag tgatcgagac aaattggcct cctgtctgac tctttttatc cagatgggcc
    29101 ccgtttcagc tccagaacgt ctacaaatca aaacttttgc gccctctttt tactttgaca
    29161 gggaagaaga taatcggatt cgtttagaaa ttcagtttga ttatggaaat agacaggtat
    29221 ctagccgaca agagctagaa gaattaccat tttcaagtga cgcggactta gaagaaagaa
    29281 ttttccaagt ctgtttggct gcgggctttg aagccgattt tcaatcttgg cgtcaggcct
    29341 taaaagcgga gtctgtctat catttttttc atgatatcat tccagtcttt gaaaaactcg
    29401 ggcatgttga cctatcagat aaactagaag agctttatag cctagcgagt cctcaggtgc
    29461 aaattgcctc caagggaggt ctcttggaaa tccagtttga ttttcaagat attgcccagg
    29521 aggaaatcga ccaagccatg caggccttgg ttgccaatca ggatttttat attgattcgt
    29581 ctaatcaagt ttactttttc gatgaagaaa ccaagaaaat tcgccaaaat ctacaggaac
    29641 tggggcaatt tgaattaaaa gatgggacct tgcaggctcg gaaatccttg gcctacagtt
    29701 tagctcatct ctttgaaggg cgagaccgtg tttctttttc acaagaattc cagaatctgg
    29761 cccaggattt gacgcatcca gaggactttc ctttgcaagc aactcaggtc aaggctgact
    29821 tgcgagatta tcaggaaaag ggaattggct ggttgcagat gctccatcat tatggttttg
    29881 gtgggatttt ggctgatgat atgggacttg ggaaaaccct tcagaccatt gcttttttga
    29941 ctagtcaagt gacaaaagaa agtcgggttt tgattctcgc tccgtcgggt ttgatttaca
    30001 actgggcaga tgagtttcag aaatttgctc cacagttgga tgtgactgtt gttcatggtt
    30061 tgaaagcaag tcgtgaggag attcttgccg agagccatca aatctatgtg actagctatg
    30121 ccaccttccg tcaggacagt gagttttatc aagggatggc ctttgacttc cttttcttag
    30181 atgaggctca ggtcatgaaa aatgcccaga ccaagattgc ccagaccttg agacaatttg
    30241 tggtgccgtc ggtctttgcc ttgtcaggaa ctccgattga aaaccatctg ggtgagttgt
    30301 ggtctatttt ccaaatcgtc atgcccggac ttttgccaag caagaaagaa tttatgaaat
    30361 tgccagcaga gcgagtggct cagtttatca agcctttcgt gatgcgacgc aagaaagaag
    30421 aagttctgac tgaattgcca gacttgattg aggtggttta taaaaatgaa ctggaagacc
    30481 agcaaaaggc tatttacctt gcccagttac aacagatgcg agaccatctg gctcaagtgt
    30541 cagaacagga atttcagcga agtcgtgtgg aaattttatc tggtttgatg cgcttgcgtc
    30601 aaatatgtga cactcctgcc ctgtttatgg aagattatca gggagccagc ggcaaactag
    30661 atagtctccg agacctattg gtacaggtgg cagacggcgg acaccgtgtc ttgattttct
    30721 ctcagttcaa gggaatgttg gaaaaaattg aacaagaact gccagacttg ggcctgactt
    30781 cctttaaaat tacgggttca accccagcca aggaaagaca agacatgacc aaggccttta
    30841 accaaggaga aagagatgcc tttctgattt ccctcaaggc tggtggggtc ggtctgaacc
    30901 tgacaggtgc agatacagtg atcttggttg acctttggtg gaatcctgcg gtggaagcgc
    30961 aagccattgg ccgtgcccat cggatgggtc aggaagaaac ggttgaggtc tatcgcttgg
    31021 tgaccaaggg aaccattgaa gaaaaaattc aggaactcca agaacaaaag aaacatctgg
    31081 tgtcacaagt attggacggc acagagtcac gtggtagtct gaccctagca gaaattagag
    31141 aaattttggg aatttctgaa gccagcactt gaaaaatcaa gacaatacgc tataatgaag
    31201 tgttgaaagg aaatagaaaa tgaaacaaga acgatttcca ttggtgtcag atgacgaggt
    31261 catgttgact gaaatgccag tcatgaatct ctatgatgag tctgatctga tcagtaatat
    31321 caagggtgag tatcgagata aaaattattt agaatgggct cctattgctg aagaaaaacc
    31381 agtaaaaccg attgaaaagc aagtcgaaaa acctaaaaag gctcctttag gggttaaaaa
    31441 agaaggaaag agctatgcgg aggtggcgcg tgaagaagcg cgtgcggact tgaaaaagaa
    31501 acgctctgct aactacctaa ctcaggattt cagccttgcg agacgtcatt ctcagcccag
    31561 tctagttaga cagggcaatc aaccgacagc tcctttccaa aaggaaaatc ctggtgaatt
    31621 tgtcaaatat agccaaaaat tgacccagtc tcattatatc ttggcggaag aagttcattc
    31681 tatccctacc aagaatgaag aagtgtcagc acctgctcca aagaaaaaca attatgattt
    31741 tctaaagaag agccaaatct acaataaaaa aagtaaacaa acagaacaag aacgtcgggt
    31801 tgcccaagag ttgaatctga ccagaatgac agaatagggg agaaaacatg tcaaagacat
    31861 atcattttat cggaattaag ggatcaggga tgagtgcctt ggccttgatg ttgcaccaga
    31921 tggggcacaa ggttcaggga tcagatgttg aaaagtacta ctttacccaa cgcggtcttg
    31981 agcaggcagg aattaccatt cttccttttg atgaaaaaaa tctagacggt gatatggaaa
    32041 ttatcgctgg aaatgccttt cgtccagata acaacgtcga aattgcctat gcggaccaaa
    32101 atggtatcag ctacaaacgt taccatgagt ttctaggtag ctttatgcgt gactttgtta
    32161 gcatgggagt agcaggagca catggaaaaa cttcaacgac aggtatgttg tctcatgtct
    32221 tgtctcacat tacagatacc agcttcttga ttggagatgg gacaggtcgt ggttcggcca
    32281 atgccaaata ttttgtcttt gaatctgacg aatatgagcg tcacttcatg ccttaccacc
    32341 cagaatactc tattatcacc aacattgact ttgaccatcc agattatttc acaagtctcg
    32401 aggatgtttt taatgccttt aacgactatg ccaaacaaat caccaagggt ctttttgtct
    32461 atggtgaaga tgctgaattg cgtaagatta cgtctgatgc accaatttat tattatggtt
    32521 ttgaagctga aggcaatgac tttgtagcta gtgatcttct tcgttcaata actggttcaa
    32581 ccttcaccgt tcatttccgt ggacaaaact tggggcaatt ccacattcca acctttggtc
    32641 gtcacaatat catgaatgcg acagccgtta ttggtcttct ttacacagca ggatttgatt
    32701 tgaacttggt gcgtgagcac ttgaaaacat ttgccggtgt taaacgtcgt ttcactgaga
    32761 aaattgtcaa tgatacagtg attatcgatg actttgccca ccatccaaca gaaattattg
    32821 cgaccttgga tgcggctcgt cagaaatacc caagcaagga aattgtagca gtctttcaac
    32881 cgcatacctt tacaagaacc attgccttgt tggacgactt tgcccatgct ttaaaccaag
    32941 cagatgctgt ttatctagcg caaatttatg gctcggctcg tgaagtagat catggtgacg
    33001 ttaaggtaga agacctagcc aacaaaatca acaaaaaaca ccaagtgatt actgttgaaa
    33061 atgtttctcc actcctagac catgacaatg ctgtttacgt ctttatggga gcaggagaca
    33121 tccaaaccta tgaatactca tttgagcgtc tcttgtctaa cttgacaagc aatgttcaat
    33181 aggatgttcc catggaaatt ccaattaaga tcattcaggc aagcaagtct gatttgcctg
    33241 agataggggc acttcagacc tcgtcttttc cagctgaaaa gcagcaactt tcccatattt
    33301 tagaagaaag tatccgtaag tgtgcggata cctttcttct agctagggat gaaaatcaac
    33361 ttttaggcta tattttatca agtccccagt cagacaatcc gcaatgtcta aaagtacatt
    33421 ctttagtcat cgagtctgac catcagagac agggcctggg aacacttctt cttgcagcct
    33481 tgaaagaggt ggcagttgag ctggattaca aagggattcg tttggagagt cctgatgagc
    33541 tgctttccta ttttgaaatg aacggttttg ttgatgaaga agcaactttg ctctatgcaa
    33601 ctagccaggg ctatagtatg atttggttta atccctttta tctggaggaa caatgaaaat
    33661 cagacaagca agattagaag atttggatcg gattgttgaa cttgaatttg aaaatttttc
    33721 ggtcgaagaa gccattcctc cttctgtctt tgaagcacat ttgagagaaa ttcagacctc
    33781 ttttctggtt gctgaaaaag aaggaagaat catgggttat atcgaaggac cagttggcct
    33841 gcaccgccat ctgcaagacc agtcttttac agaagaaata aaagactata gtcatgagcc
    33901 tggtggttat atctctgtga cctgtctgtc tattgccaag gaggcacagg gactcggact
    33961 gggtcagaaa ttgctgacag ccttgaaaga agttgctctt gaagatgaga gagatggcat
    34021 taatctaacc tgtcatgact atctcatcgc ctactatgaa aaacatggat ttgtcaacga
    34081 aggccagtcc cagtcaacct ttgcagggga aacatggtat gatatggtct gggaaatgaa
    34141 aaaataagtt aggaaaagta tcatagattg ctttttttgg acttttaaga tataatatta
    34201 tggattatca acaaggagga aaaacttttg agtgaaaagt caagagaaga agagaaatta
    34261 agctttaaag agcagattct gagagattta gaaaaagtaa aaggctatga tgaagttctg
    34321 aaagaagatg aggcagtagt tcgcactcct gcaaatgaac cttcaactga agaactcatg
    34381 gctgattcct tgtcaacggt agaggagatt atgagaaaag ctcctaccgt gcctactcac
    34441 ccaagtcaag gtgtaccagc ttctccagca gatgagattc aaagagaaac tcctggtgtt
    34501 ccaagtcatc caagtcaaga tgtaccttct tctccagcgg aagaaagtgg atcaagacca
    34561 ggtccaggtc ctgttagacc taagaaactt gaaagagaat acaatgaaac cccaacaagg
    34621 gtagctgttt cctatacgac ggcagagaaa aaagcagaac aagcaggtcc agaaacacct
    34681 acgcctgcta cagaaacagt ggatatcatc agagatacat cacgtcgtag ccgtagagaa
    34741 ggagcaaaac ccgttaagcc taagaaagag aagaagtcac atgtgaaagc ttttgtgatt
    34801 tcattccttg tattccttgc cttgctctca gcaggtggtt actttggtta ccagtacgtg
    34861 ctagattcct tattacctat cgatgctaat tctaagaaat atgtgacggt tggaattcca
    34921 gaaggttcaa acgttcaaga aatcggtacg acgcttgaaa aagctggttt ggtaaagcat
    34981 ggtctgattt ttagttttta tgccaagtat aaaaattata ccgacttgaa agcaggttac
    35041 tacaatttgc aaaagagtat gagtacagaa gacttactca aagagttgca aaaaggtgga
    35101 acagatgaac cgcaagaacc tgtacttgcg actttgacaa ttccagaagg ttataccttg
    35161 gatcagattg ctcaaactgt gggtcaattg caaggtgact tcaaagagtc tttgacagcg
    35221 gaggctttct tggctaaagt tcaagatgag acgtttatca gtcaagcagt agcgaaatat
    35281 cctactttac tggaaagttt gcctgtaaaa gacagcggtg cgcgttatcg tttggaagga
    35341 taccttttcc cagctacata ctctatcaag gaaagcacaa ctattgagag cttgattgat
    35401 gagatgttag ctgctatgga taagaaccta tctccttact atagtactat caaatctaaa
    35461 aacttgactg tcaatgagtt gttgaccatt gcttccttgg tcgaaaaaga aggtgccaag
    35521 acagaagatc gtaagctcat tgcaggtgta ttttacaatc gtttgaatcg tgatatgcca
    35581 cttcaaagta atattgcaat cttgtatgcc caaggaaaac tggggcaaaa tatcagtcta
    35641 gctgaggatg ttgcgattga taccaacatt gattcacctt ataatgttta taaaaatgta
    35701 ggtctcatgc ctggtccagt cgatagtcca agtctggatg cgattgagtc aagcatcaat
    35761 caaactaaga gcgataacct ctactttgta gcagatgtca cagaaggcaa ggtctactat
    35821 gctaacaatc aagaagacca cgaccgcaat gtcgctgaac atgtcaacag caaattaaac
    35881 taaacaaact aaaattatgt gatacttcac ataattttct ttagaaaata ttatcagaag
    35941 aaagttgaga aaaatggcag aaaaaacata tcctatgacc cttgaggaaa aggagaaact
    36001 tgaaaaagaa ttagaagaat tgaaattggt tcgtcgacca gaagtggtag aacgcattaa
    36061 gattgcccgt tcatacggtg atctttcaga aaacagtgag tacgaagcag ctaaggatga
    36121 acaagccttt gtcgaaggac aaatctctag cttagaaaca aaaatccgct atgctgaaat
    36181 cgtcaatagc gacgcagttg cccaggacga agtagcgatt ggtaaaacag tcaccatcca
    36241 agaaattggt gaggacgaag aagaagttta tattatcgta ggttcagctg gtgcggatgc
    36301 ctttgcaggt aaggtttcaa atgaaagccc aattgggcag gccttgattg gcaagaaaac
    36361 aggtgacaca gcaaccattg aaacgcctgt tggtagctat gatgtaaaaa tcttgaaggt
    36421 tgaaaaaaca gcctaaaaac agaaaaagga gtggggaggc gatgtgcttc actcactcct
    36481 ttttccattt tgctattctt cgaaaatctc ttcaaaccac gtcagcgtcg ccttgccgta
    36541 tgtatggtta ctgacttcgt cagtttcatc tacaacctca aaacagtgtt ttgagctaac
    36601 ttcgtcagtt tcatctacaa cctcaaaaca gtgttttgag ctaacttcgt cagtttcatc
    36661 tacaacctca aaaccatgtt ttgagccgac ttcgtcagtt tcatctacaa cctcaaaacc
    36721 atgctttgag ctgacttcgt cagtttcatc tacaacctca aaaccatgct ttgagctgac
    36781 ttcgtcagtt ctatctacaa cctcaaaaca gtgttttgag caacctgcgg ctagtttcct
    36841 agtttgctct ttgattttta ttgagtatta gatgtatagt gcattgaagt taaaaatagt
    36901 acactttgat ttctaagaca ttgttagaaa ttagattaaa ttccttaatc gatttgcgca
    36961 tgttttattt catttcacta tactttagat tggaaaataa ggaatcacat aaatcgcaag
    37021 aaatagggaa tctgattcgt gtttataacc gttcaaaaag agaagaggct gaaagtgagc
    37081 cacttaatct ttatgtcgaa gatgaaaagg gcaatctcct ggcaggtttg atagcagaga
    37141 cttttggaaa ttggctagaa atcgagtatt tgtttgtaaa agaggaactg cgagggcaag
    37201 gaatcggttc aaaactattg caacaggcag aaagtgaagc taagaatcga aattgttgtt
    37261 ttgcctttgt caatacttac cagttccagg caccggactt ttatcagaaa catggctaca
    37321 aggaagtttt ttctttgcaa gattatctct acattaggca aagatattat taccaaaaga
    37381 atctgtaagg tgagtatgga agtggactga tatatatgag taactatcga taaataccag
    37441 ttataaccta gaaattgtgt ctttctagac gatattgagg acaatataat cgtagcccca
    37501 aaattaggaa ttaaggctta tcaggttaag aaaagaagtg atgttgttga tattttaaaa
    37561 caatttattt aaactaaaaa ctctctatct ttttaaattg acattaatcc gagattcccc
    37621 tctcggattt tgtgttgctt tcattaaatt tacaggtaag acttgctagt ttcagtaaat
    37681 atacacaagt ttttcagaat agagtgaaat ccaaaagaaa atggaaataa tgaaagctct
    37741 tgtaggcata agaaaacacc tctgtgttat acttgttgtt caaccacaaa cacaagaaag
    37801 gcacagagat gcaagacaat tatactacaa aagccaaaca tttgacaatc gatagccgtc
    37861 gcttaatcga aagatggaaa aaagaaggga aatcaaatag agaaattgcc tctctacttg
    37921 gaaaag
//
LOCUS       DAVKGC010000006        36067 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKGC010000006 DAVKGC010000000
VERSION     DAVKGC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36067
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.006"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(121..1755)
                     /locus_tag="VW049_000247"
     CDS             complement(121..1755)
                     /locus_tag="VW049_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431434.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1736..2485)
                     /locus_tag="VW049_000248"
     CDS             complement(1736..2485)
                     /locus_tag="VW049_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431435.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            2664..4118
                     /locus_tag="VW049_000249"
     CDS             2664..4118
                     /locus_tag="VW049_000249"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9431436.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVHSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4208..6826)
                     /gene="alaS"
                     /locus_tag="VW049_000250"
     CDS             complement(4208..6826)
                     /gene="alaS"
                     /locus_tag="VW049_000250"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9431437.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6848..7333)
                     /locus_tag="VW049_000251"
     CDS             complement(6848..7333)
                     /locus_tag="VW049_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9431438.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7608..7847)
                     /locus_tag="VW049_000252"
     CDS             complement(7608..7847)
                     /locus_tag="VW049_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431439.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8100..9170)
                     /locus_tag="VW049_000253"
     CDS             complement(8100..9170)
                     /locus_tag="VW049_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9431440.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9167..9940)
                     /locus_tag="VW049_000254"
     CDS             complement(9167..9940)
                     /locus_tag="VW049_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9431441.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9937..10743)
                     /locus_tag="VW049_000255"
     CDS             complement(9937..10743)
                     /locus_tag="VW049_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9431442.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWIMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDEMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10724..11881)
                     /locus_tag="VW049_000256"
     CDS             complement(10724..11881)
                     /locus_tag="VW049_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431443.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12042..12947)
                     /gene="murB"
                     /locus_tag="VW049_000257"
     CDS             complement(12042..12947)
                     /gene="murB"
                     /locus_tag="VW049_000257"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9431444.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13059..13811)
                     /locus_tag="VW049_000258"
     CDS             complement(13059..13811)
                     /locus_tag="VW049_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9431445.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13821..14552)
                     /gene="budA"
                     /locus_tag="VW049_000259"
     CDS             complement(13821..14552)
                     /gene="budA"
                     /locus_tag="VW049_000259"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9431446.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14694..15965
                     /locus_tag="VW049_000260"
     CDS             14694..15965
                     /locus_tag="VW049_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9431447.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15985..16800)
                     /locus_tag="VW049_000261"
     CDS             complement(15985..16800)
                     /locus_tag="VW049_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9431448.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(16957..17610)
                     /gene="phoU"
                     /locus_tag="VW049_000262"
     CDS             complement(16957..17610)
                     /gene="phoU"
                     /locus_tag="VW049_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9431449.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17622..18380)
                     /gene="pstB"
                     /locus_tag="VW049_000263"
     CDS             complement(17622..18380)
                     /gene="pstB"
                     /locus_tag="VW049_000263"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9431450.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18393..19196)
                     /gene="pstB"
                     /locus_tag="VW049_000264"
     CDS             complement(18393..19196)
                     /gene="pstB"
                     /locus_tag="VW049_000264"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9431451.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19207..20091)
                     /gene="pstA"
                     /locus_tag="VW049_000265"
     CDS             complement(19207..20091)
                     /gene="pstA"
                     /locus_tag="VW049_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9431452.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20081..20998)
                     /gene="pstC"
                     /locus_tag="VW049_000266"
     CDS             complement(20081..20998)
                     /gene="pstC"
                     /locus_tag="VW049_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9431453.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(20998..21876)
                     /gene="pstS"
                     /locus_tag="VW049_000267"
     CDS             complement(20998..21876)
                     /gene="pstS"
                     /locus_tag="VW049_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9431454.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22450..23754)
                     /locus_tag="VW049_000268"
     CDS             complement(22450..23754)
                     /locus_tag="VW049_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9431455.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23755..24528)
                     /locus_tag="VW049_000269"
     CDS             complement(23755..24528)
                     /locus_tag="VW049_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9431456.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24518..24796)
                     /locus_tag="VW049_000270"
     CDS             complement(24518..24796)
                     /locus_tag="VW049_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9431457.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24796..25197)
                     /locus_tag="VW049_000271"
     CDS             complement(24796..25197)
                     /locus_tag="VW049_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9431458.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25407..26024)
                     /locus_tag="VW049_000272"
     CDS             complement(25407..26024)
                     /locus_tag="VW049_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9431459.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLAQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(26014..26787)
                     /locus_tag="VW049_000273"
     CDS             complement(26014..26787)
                     /locus_tag="VW049_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9431460.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRGIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26798..27535)
                     /locus_tag="VW049_000274"
     CDS             complement(26798..27535)
                     /locus_tag="VW049_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9431461.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSVVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(27540..28670)
                     /locus_tag="VW049_000275"
     CDS             complement(27540..28670)
                     /locus_tag="VW049_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9431462.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28754..29143)
                     /locus_tag="VW049_000276"
     CDS             complement(28754..29143)
                     /locus_tag="VW049_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9431463.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQICSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29159..29542)
                     /locus_tag="VW049_000277"
     CDS             complement(29159..29542)
                     /locus_tag="VW049_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9431464.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29543..30331)
                     /locus_tag="VW049_000278"
     CDS             complement(29543..30331)
                     /locus_tag="VW049_000278"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9431465.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGFRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30324..31259)
                     /locus_tag="VW049_000279"
     CDS             complement(30324..31259)
                     /locus_tag="VW049_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9431466.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31389..31565)
                     /locus_tag="VW049_000280"
     CDS             complement(31389..31565)
                     /locus_tag="VW049_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9431467.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31645..32154)
                     /locus_tag="VW049_000281"
     CDS             complement(31645..32154)
                     /locus_tag="VW049_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431468.1"
                     /translation="MNKEEFLKIKEAYKSARTEERKSIIGFITKKKDKEGNFLFTKSK
                     DKPYTTRNQYSGGGGNKKYTSGSRLSRPYDLSNHMWIDLSYKGNDILISLQSFDIDPN
                     SKELHVLYDRIGILFEQSKKIPIFKDCYTITKVSDAFLKMETTNWELPLSEADMEEMV
                     NYIINHYEE"
     gene            complement(32232..32939)
                     /locus_tag="VW049_000282"
     CDS             complement(32232..32939)
                     /locus_tag="VW049_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9431469.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLYFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHVVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            33090..34118
                     /gene="queA"
                     /locus_tag="VW049_000283"
     CDS             33090..34118
                     /gene="queA"
                     /locus_tag="VW049_000283"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9431470.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34779..35168)
                     /locus_tag="VW049_000284"
     CDS             complement(34779..35168)
                     /locus_tag="VW049_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9431471.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(35222..35914)
                     /locus_tag="VW049_000285"
     CDS             complement(35222..35914)
                     /locus_tag="VW049_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9431472.1"
                     /translation="MNTSFAHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRFEKDETKDRSENHVTIDGEDGYVTTTRTYDVNPETGHVTEKVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEISIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
BASE COUNT        10730 a         8213 c         6814 g        10310 t
ORIGIN      
        1 cttattcaaa acattcctcc ctatattttt gattaccaat cttaatcatt tacaactaca
       61 ttctaacaaa ctataaaagc gtttgtcgaa ttgaatttat caagcaagca accaactatc
      121 tcatcttttt tctatttctg ccaatatgcg tgacaggtaa taatgatagc caaaaatagc
      181 aagagcaagc aagacgataa gagctcctac tcccaagttg atggcaagga taggggagag
      241 agactgaacc aagaatatgc tcccaattac aagggccatc aggattgcac tataaataaa
      301 caataaaact atggcgacta tgccatttga acgattcacc aggtccgtaa tgctactcca
      361 attggttgac agatttttaa cgtccttaaa gtaatggtgg caagaaagga tgacactggc
      421 aatgatccag actacaagaa ggtaaatcat cgaaaggatg gacaagccta gatatagaga
      481 aagaccgagc aaagtcagaa ctggtaaaaa ggactgaaca gcaaagataa tccaaaattt
      541 cactttcaca taacgagcaa agtcaaaggg taaactcttc agaaaatcaa cattttccct
      601 ctccaaggac aaggcaattg aatgcaggct ggtgatattg ttattgacaa ctgctataaa
      661 cactcctaca aaaaacaagg gtaaccagta tggaggatga atgtctggaa ctatctgaga
      721 atctcggatt ttggaaatca gactgatcat catgagataa ggaaggaaag cacttgtaaa
      781 aagcactgta atcacaccag tcccctgtcc caagagggtg aggtggtagc gtaaaaccat
      841 acggaaaaag ccctttttag tagttgaaat cctctccttg ctgcgacgtt cttttttgac
      901 cttctcctca ctgttaagca ggatcacgtc ataaaaacga ggaaggacct tctttttggt
      961 cagataaagc aggaagagag ttagtcctat ccaagcgagc agacccacta aggcttctgt
     1021 cgaaaaaggc tccactgcta ttttgtaaaa gatatgaaga ggataaagga gaaatggaat
     1081 gtctctaact ttgtcaacaa tacttccaaa agtcgactga agaaagaaga taaatattaa
     1141 aggtatgaga actcctatcc caatcatcac attcgaaaaa atagactgat actttctgaa
     1201 gaccctagtc tgagccaaga aatgtactgc cactaccgtc actaaagtaa cagagacaaa
     1261 taataaggtc aaggacagta gcatcaaagg caaacccagc caaagagaag gagctagact
     1321 aatatagagg gctagaaaat aagctaggat tggtacaatt ccagttagag ctggcaagag
     1381 gacagacaga cctttggcca ttataatctc tgattcttta aaggcatagg gcctatagga
     1441 ggccaggtcc ttactctcat aaaagacatt gtaaaaggca gtgaaagaag ttgaaaaggc
     1501 cagcaccagt aaaatagcaa ccatcgagct aaaataaata ggcatttcct caaaaggaaa
     1561 atgaatggct atattactaa aacagatgag catcaagaga ctggaaaaaa tgtaagaact
     1621 taggactcta gcagaaacat ttactttttt cccaggattc ttagcctgct tctttcgtag
     1681 attagccaga ttagcttctt gagatgaata gaggatattg atatcaacta attttttaat
     1741 gaccttgaga cgcatccgca acctcctctt ttctaccagc aagactaagg tagatacttt
     1801 ccaaaaactg gtctgggtgg tctttcctca agttctctac actaccacaa tagatcaaat
     1861 gccccttttt caaaatagca atccgatcac agacttgctc ggctacctca aggacatgag
     1921 ttgaaaacaa gaccgtcttg cctttttgtg catgttcctt catcatttgc ttcaaatcaa
     1981 aggcggcctg gggatccaaa ccagtcaagg gttcgtccaa aacccaaata tcaggagcag
     2041 acaagagtgc cccaatgaca aagactttct gacgcattcc gtgagaaaga gtttcaataa
     2101 cctgatagcg gttttcagca aaatcaaaaa cgttcaatag cctagctaga ctagtctcca
     2161 agtcagagct agtcagatca taggatgaag cgatcaattc ccaaaattca ttggccgtta
     2221 agcgtaaaaa taagtcaggc gagtctgcta cgtagccaat ctttcgtttg atcgccaagc
     2281 gattttccga taactcctga ccgtctacca aaatacgacc actgctgggt gaaatgatac
     2341 tgactaggga ttttatggtg gtcgattttc cagccccatt atgaccaatc aagcccataa
     2401 tctctccatt ttcaatctgc aaattgagat tactcaaggc ctctttatca ccatacaact
     2461 tactaacatt ttgaaattca atcatgggac aactcctatc tttatctgta ttatacatcg
     2521 ctatactagc actttgatac aaaaaaatca atactttatt ttaagtagtt aaaatagttt
     2581 ccttctatct tacgcaaacg tttgcgccga gagatacttt atgatagaat aaagaacaag
     2641 attgacaagt aagaggaaac atcatgcaaa atcaaacact catgcaatac tttgaatggt
     2701 atctgcccca cgacggtcaa cactggacgc gtctggctga aaatgctcca cacctagctc
     2761 atctggggat cagtcacgtc tggatgccac cagccttcaa ggcaaccaac gaaaaagatg
     2821 tcggctatgg ggtctatgac ttatttgact taggagagtt caaccaaaaa gggactgtac
     2881 gcaccaagta tggtttcaaa gaagactatc ttcaagccat tcaagccctt aaagaacagg
     2941 gaattcaacc tatggccgat gtagttctca accacaaggc tgctgccgat aacagggaag
     3001 cctttcaggt tatcgaagtt gatcctgtag accgtacagt tgaacttgga gaacccttca
     3061 ccatcaatgg ctggactagt tttaccttcg atggtcgcca agatacctat aatggcttcc
     3121 actggcattg gtaccacttc accggtacag actacgatgc caaacgcagt aaatctggga
     3181 tttatctgat ccaaggggac aacaagggct gggctaacga ggaattggtc gataacgaaa
     3241 acggaaacta cgactacctc atgtatgccg acctagactt taaacatcct gaagtcatcc
     3301 aaaacatcta tgactgggct gattggttca tggaaacgac tggtgtagct ggtttccgtt
     3361 tggatgccgt taagcatatt gactctttct ttatgcgcaa tttcatccgc gatatgaagg
     3421 aaaaatacgg tgacgatttc tatgtttttg gtgaattttg gaacccagac aaggaagcca
     3481 atctggacta tctcgaaaaa acggaagaac actttgacct tgtcgatgtt cgtctccacc
     3541 agaatctctt tgaagccagt caagctggcg caaactatga ccttcgtggc attttcacag
     3601 atagcctggt tgaactcaag cctgacaagg ctgtgacttt tgtcgacaac cacgataccc
     3661 aacgaggaca agcccttgag tctaccgttg aagaatggtt caagccagca gcctatgccc
     3721 tcattttgtt acgccaagac ggccttccat gtgtctttta cggagactac tatgggattt
     3781 caggccagta tgctcaagaa gatttcaaag aaatccttga ccgcctccta gccatccgaa
     3841 aagacttggc ctatggagaa caaaatgact actttgacca tgctaactgt atcggttggg
     3901 tacattcagg tgctgaaaat caatccccaa tcgcagtcct tatctcaaat gaccaagaaa
     3961 acagcaagtc aatgtttgtc ggtcaagaat ggactaatca aacctttgta gatttacttg
     4021 gtaaccacca aggtcaagtt acaattgatg aggaaggtta tggacaattc cctgtctcag
     4081 ctagatccgt aagtgtctgg gcagtcaata ccatctaata gctcataata accaagctag
     4141 gtccaagcgg atttggcttt tttgtattca caaaaagacc tacccaaatg gatagatctt
     4201 tacttgatta caatttacct gctactgcat ccaacaattc ttggatctta gcttggttgc
     4261 ttcctcctgc catggccatg tctggtttac caccaccacg tccatcgatg attggtgcta
     4321 attctttgac aaggtttcct gcatgaaggt cttttgtctt gcttgctaca aggacattga
     4381 ctttgtcacc gatagcggca actaggacaa gaagatcaga gtagtctttt tgtttccagt
     4441 tatctgcaaa agtacgaagg gcaccggcat cggatacaga cacttgacta gcaatgtaac
     4501 gatgaccgtt gacttcctta gcatctttga agatatcgcc tgcggctgca gctgcggctt
     4561 tttctttcaa ctcagcattt tctttttgaa gttgacgaag ttgttcttga agtccttcta
     4621 ccttgtgagg tacttccttg acttgaggtg ctttcaaggt tgctgcgaca gctttaagag
     4681 catcctcttg ttcacgatag gcttcaaagg cttccttacc agtcactgcc aagatacggc
     4741 gagttcctga accgattcct tcttctttga caattttgaa gagaccaatc tcagaagtgt
     4801 tgccaacatg agtaccacca caaagttcaa tagagtagtc accgatagtc acgacacgaa
     4861 cttccttgcc gtatttctca ccaaagaggg ccatagctcc catttcttta gcagtgtcaa
     4921 tatccgtttc aactgtcttc acttcaagtg cttcccaaat tttctcgtta acttgctgtt
     4981 caatggcacg aagttcctca gcagttactg cttggaagtg ggtaaagtca aagcgaagga
     5041 attcaacttc gttaagagat cctgcctgtg ttgcgtggtt tccaaggata ttgtgaaggg
     5101 cagcgtgaag caaatgagtc gcagtgtggt ttttcatgac acggtgacgg cgattgctat
     5161 caattgccaa ggtatattct tggttcaagg caagcggtgc aaggacttca actgtatgaa
     5221 gggcttgacc atttggggct ttctgaacat tggtcacagt agccacaatc ttacctgact
     5281 catccaagat ttgtccgtag tcagctacct gtccacccat ttcagcataa aatgacgttt
     5341 ccgcaaagat aagagaggca gttccttctg aaacagctcc tacttctgca ttgtcagcaa
     5401 cgatagctac caatttagaa gacaattggc tagtattgta gttgaagaca ctttctacag
     5461 tgatgttttg aagagtttca ttttgcatac ccattgagcc acccttgaca gctgacgcac
     5521 gcgcgcgttc ttgctgttct ttcatggctg cttcaaaacc ttcacggtct acagtcatac
     5581 cagcttcttc agcaatttct tcagtcaatt caactgggaa tccatatgta tcatagagtt
     5641 taaagacatc ttgaccagca atgacagatt gacctttttc tttcaagtct gccacaatgc
     5701 cttgggcaaa gtgttgacct gagtgaaggg tacgggcaaa tgattcttct tcgctcttaa
     5761 cgattttctc aataaagtca cgtttctcaa gcacttctgg gtagtagctt tccatgattt
     5821 ttccaacagt tggaaccaat ttgtaaagga aaggctcgtt gattcccaat ttttgaccat
     5881 gcatagaagc acgacgaaga agacgacgaa ggacataacc acgaccttca tttcctggaa
     5941 gggcaccatc accgatggca aatgaaagtg aacggatgtg gtcagcgatg accttgaagc
     6001 tcatgttgtc gccatcttgg tcataaacct taccagacaa tttctcgact tcacggataa
     6061 tcggcatgaa gaggtccgtt tcaaagttgg tcttagcccc ttggataacg gccaccaaac
     6121 gctccaaacc agcgcccgta tcaatgttct tatgtggcaa ttccttgtat tcgctacgag
     6181 gaacagcagg gtctgcgtta aattgtgaca aaacgatgtt ccagatttca atataacggt
     6241 cgttttcaat atcttctgca agcaggcgaa gaccgatatt ttctgggtca aaggcttccc
     6301 cacggtcaaa gaagatttct gtatctggtc cagaaggtcc cgcaccaatt tcccagaagt
     6361 tgtcctcaat tggaatcaag tgacttggat ccactcccac ttcaatccag cggttgtaag
     6421 aatctttatc atctggatag taagtcatgt aaagtttctc agctgggaag tcaaaccatt
     6481 cagggcttgt caaaagctca taagcccaag tgatggcttc atcacggaag taatccccga
     6541 ttgagaagtt acccaacatt tcaaacatgg tatggtgacg tgcagtcttc cctacgttct
     6601 cgatatcgtt agtacgaata gccttttggg cattggtaat acgtggattt tcagggatga
     6661 tagtcccgtc aaagtatttc ttaagagttg ctaccccaga gttgatccac aaaagagttg
     6721 ggtcatttac aggaaccaaa cttactgatg gttctactga gtgacctttg gtcgcccaga
     6781 aatcaagcca catttggcgt acttgtgcac tagatagttg tttcatattg tctccttatt
     6841 tacttgttta atgtgattgg ctttccagca tttccacata gtcaatcgcg acacagaggg
     6901 aaatgactag gtctgcataa gcgtcttcaa gaaccgttac ggtataggta gaggtcagat
     6961 ggaagagttc cttcttaatt tccgcaatca actgatcgcg atcatccagc aatttgaaat
     7021 tcaaatccca gatattgccc tcgatacgaa gacctagatt atcaaactca tacttatctc
     7081 gccagaaggt caacttctta cgaatgacaa aactcgagcc atcccgaagc tgaatctcaa
     7141 aacgaggaag caaggtcaag atttctttac tgatctgact gacttgttca ccagccgcat
     7201 catagatggt aaaagttttg ggaatcttaa aaaatgatcc ctccacctga taggcaattt
     7261 ctcccctgtc atccttgata gcgaagcgtt cgcctccaag acgaaacttt tgtttgacaa
     7321 gaaatgtttt catcaacacc tccaaaaatc aaaagacaag ctcatatcac gaagggcgaa
     7381 aaaccgcggt accaccttca ttcaatgaac ttgtcattct cttgttctta tgcaattgta
     7441 tgattgagta gcatgacttc ctagcttaga tggctcgcag caccgccatt tctctggact
     7501 aagacaagtg aaaatcaatt ctcaactttc ttattataac gtttttttaa gcttgcgtca
     7561 actggaaatg atctccgttg aattagacca attccctaca tctctgatta ctttttcagg
     7621 atatattttt tcttactgcc atttttcttt ttatcccaaa ttttcatatt actaaacaca
     7681 gctactagaa tatttccaaa tataaaggtg cctatcaccc aatatatgga ctcagttgtt
     7741 aggtattgtc gatccaagcc atcctttaaa tggaatagta tagcagtttg gttaacaatc
     7801 ataaaggttg gccagaaact ttttttgaaa aaagtagaca ttttcattat ttgttgccgc
     7861 tttctgtaag gttaatactc aataaaaatc aaaaagcaaa ctaggaagct agcctcaagc
     7921 tgtacttgag tacggcaagg caacgctgac gtggtttgaa gagtataggc taagtatact
     7981 actaggcaag caaataagca aataagcaac tagaatagaa aaagataggg ctctaaaaac
     8041 tgacttctat tccttaaaaa cgaaccagct tgactgattc gtcttcttac gtttatctcc
     8101 tacttccgat acattttaaa ctgtaggaag aggtcgctat atttccctgt ccatttatgg
     8161 tcaaatttct cataaacttc taggtgtttc atggtttcaa catcgggata gaaggcctta
     8221 tcttcctttg tttcctctgg gagcaattcc ttcgctggta ggtttggtgt tgaatagccg
     8281 acatactccg cattttggag agcattttca ggtttcaaca taaagttgat aaaggcatag
     8341 gctgagtttt ggtttttaac tgttttggga atgaccatat tgtcaaacca aagattgctg
     8401 gcctctgtcg gtaccacata acgtagattt tcattttttt ctaacatttg gctggcttca
     8461 ccagagaagg tcacgccgat tgcaacatta ttctgaatca tatagccctt catctcgtcc
     8521 gcaacgatag ccttgatatt tggagtcagt ttgtagagct tatccactgt ctcttccaac
     8581 tgctgcagat ccttggagtt gaggctgtag ccgagggaat tgagtcctag tcccagcacc
     8641 tcacgcgccc catcaaagag catgatagaa ttcttatact ccggcttcca aaggtcatcc
     8701 caatgctcag gcgcttcatc taccatggtt tcgttgtaga caattcctaa ggttccccag
     8761 aagtaaggga tggagaattt attacctggg tcaaaggact ggttgagaaa ctctggtccg
     8821 atattttcga ttccttcaat ttttgaataa tcaagcggaa ccaagaggtc ttcgtccttc
     8881 atcttgttaa tcatgtattc acttggaatg gcaatatcgt aggtcgttcc accctgcttt
     8941 atcttagtgt acatggcttc gttggagtca aaagtctcgt actgaacttg aattcctgtt
     9001 tcttctgtaa actgagtcaa gagttcagga tcgatatagt ctccccagtt atagataacc
     9061 aatttttgac tatctcgact attgatttta ctatctaaat gagtcgcaat tccccacaag
     9121 acaaggataa tcgctgcaat tcctgctaaa aatgaataga tttttttcat gcttgctcct
     9181 ccttctcacg agagataaag taataaccta caactaggat aatactaaag agaaagacta
     9241 gagcagacag ggcattgatt tctaaggaaa tccccttgcg agcacgagag taaatctcga
     9301 ctgatagggt tgaaaagcca tttcctgtta caaagaaggt cacggcaaag tcatctaacg
     9361 aataggtgaa ggccatgaaa taaccagtaa tgatagacgg agtcaggtaa ggaagcatga
     9421 tttccttgaa catctgaaat tgactagctc ccaagtcata ggccgcatga atcatgtcgc
     9481 cattcatttc cttgagtcga ggcaagacca tcaagaccac gataggaatg gagaaggcca
     9541 cgtgactaga tagaacggtc aaaaagccaa gtgaaaactt gagttgggta aagagaatca
     9601 agaagctagc accaatcata acgtcaggcg caaccatgag gatattattg agtgatagaa
     9661 aggcttcttg gtatttctta cgagactggt agatgtaaat ggcaccaaaa gtcccgataa
     9721 tggtcgctat caaggctgat aggaaggcca agaaaaatgt ctgagccaaa atcagcatga
     9781 gtctcccatc tccaaacatg gtttcaaagt gagtccagct aaaacctgta aagctattca
     9841 tatcatcacc agcattaaag gcatagccaa tcaagtaaaa aatcggcagg tagaggacca
     9901 gaaagaccag tcccagataa aggttggcaa attttttcat cgttctctcc tttccttagt
     9961 cacccacatg gtgatgaaca tggtcaggat gagaatcaca ccgatagttg aacccatacc
    10021 atagttgtca ttggttagaa aattctgctc aatagccgtc cccaaggtga taacgcggtt
    10081 cccaccaatc aaacgggtca gcatgaagag actcaaactt gggataaaga ccgactgaac
    10141 cccacttcgc acaccgttca tagatagagg gaagatgaca tgacggaagg tctcccactt
    10201 agttgcacca aggtcgtaac tagcattgat gagattatta tccatttcgt ctaagacatt
    10261 gaaaatcggc aaaatcataa aggggagctc gatgtagctt gcgacaaaga taaatgagaa
    10321 atcagtaaat agcaactgct gtgaaccaat tccgataaat tccaagaatt ggttaataga
    10381 gccattctga ccaaaaatcc cgataaaggc ataagcctta agaagaaggt tgatccaagt
    10441 tggcagaata atcagcataa tccagagttg acggtgtttg agacgggtca aaaagagggc
    10501 cgtcggataa ctgataagca gtgtcacaaa ggtcacaatg cctgcataaa gcactgagtt
    10561 gaaactcatt ttaagatagg tcaagttttg tgacgcaaag taagatttgt aattttctaa
    10621 actgaactgg ccttcgatgt tgaaaaagga ttgaccgaaa atcaagacca agggtgccaa
    10681 tacaaagagc gcaatccaaa gcatgtaggg tactacaaag agtttagagc ttgttttctt
    10741 catctctttc ctcctcgatt gcattgatca aacctgcttc ttgctcttcg atttccacgt
    10801 attcttcgat acgagcatcg aactcttctt cggtttcatt gagacgcatg atgtggatgt
    10861 cttctgattc aaagtccaga ccgatttcct cacccacgat ggccttacgg gttgagtgga
    10921 tcatccattc atttccaagt tcgtcatagg cgataatttc ataatgaact ccacggaaga
    10981 gctgggtgtc gaccttaact tggagcttgc cttcttcagg aagggtaatg cgcaagtcct
    11041 ctggacgaat aacgacctca acaggttcat ttggcttcat cccaccatca accgcttcaa
    11101 agcgtttgcc gttaaattcg accaagtagt cctcaatcat ggtacctggc aagatgtttg
    11161 actccccgat aaaggtggca acaaagtggt tgattggctc atcgtagatg tccacaggag
    11221 ttccagactg gacaatctcg ccatcattca taacaaaaat ccagtcactc atggcaagag
    11281 cttcttcctg atcgtgagtg acaaagacaa aggtaatgcc caatcgttgt tgcagttcac
    11341 gcagttcgta ctgcatgtct gttcttaatt tcaagtccag cgctgacaag ggctcatcca
    11401 acaaaaccac acggggttgg ttgatgatag cacgggcgat agccacacgc tgacgttgtc
    11461 ctccagaaag tttgcggatg gaacgttttt cataaccttc caactgaacc atcttgagaa
    11521 cttccgctac gcgttgctcg atttctttct tgtcaatctt gcgcaagcga agtggaaagg
    11581 caacattttc aaacacattc atatgcggaa acaaggcata ggattggaag accgtatgga
    11641 catcacgctt gttggttgga atatcattga tacgaacacc gtctagcatg atatctcctg
    11701 tcgtcgcatc cagtaaacct gcaataatgt ttaggatagt tgatttcccc gaaccagatg
    11761 cacctagaag ggtgtagaat ttcccttctt ccaactcaaa gttgatgtct ttgagaacct
    11821 tggtgttgct gtcttcaaaa actttagaga cgtttttgaa ttcgataatt ggttttttca
    11881 attggtataa attccttctt tttcatagat taacagatcg gggctctgtc aggtcgccac
    11941 tacctcttgc agggagtaaa accacctgca tacatcttcg ctaccgatag gctttcaccc
    12001 ccctttccat gacatagcag cagcttttca aatacagctc cctacttgct ttcacccaag
    12061 atccggactt ctctttcaag cgtaatacct gagtgttcct tgactttttc gataaccgat
    12121 tggatcaagt cctcgtagtc tttggccgtt ccatctgcga cattgatcat aaatcctgca
    12181 tgcttttctg acacttctac gccaccgata cgatagcctt tcaagccagc ttctgaaatt
    12241 aactgacctg caaaatgccc gactggacgc ttaaagaccg agccacaaga tgggtattcc
    12301 aaaggttgct tgagttcacg taggtgcgtc aagcggtcca tttcctgctt gataacctga
    12361 tgggttcctg gagctagggc aaatttaact gacaagacaa ctgcaccaga ctcctgaata
    12421 gctgaatggc ggtaaccaaa agccaagtct ttagcagaca gggtttcgat ttctccatcc
    12481 ttggtcaaga ccttacaaga ctgcaagatg tgagcaatct cgccaccata ggcacccgca
    12541 ttcataaaga cagcaccgcc aacgcttcct ggaataccac aagcaaactc aaagccagtt
    12601 aaactatgac ggagggcaat gcgagttgtt tcaatcaagt tagccccagc ttctgcttca
    12661 atggtatagc catcaacaga aacgttattg agcttgtcac acaagatgac aaatccacga
    12721 atcccaccat cacgaacgat gatattgctg gcattgccaa gaaccatcca agggatattt
    12781 tcttggttgg caaatttcac aaggcgagcc aactcaaaac gatttcgtgg aaagaccaaa
    12841 taatcagcct ctccacctac ttttgtataa ctatagctat gcaagggttc ctgaaaacgg
    12901 atatcaattc cttctaagat ttcaagcatt ttttctctta cagacatgtc actcttcctt
    12961 ttacaaaatt cattccatta taccattttt agagacattt gacgaccata aaaatacctt
    13021 gtttggattt tgcataagaa aaagaggttc cccccttttt atgatttttt acaaaagatt
    13081 tccttggttc cataggcaac cagaacgagc tccagtgcta gaatcacttc aaccaagact
    13141 ggatttgtca accagcctac ttggaaaaga gatggtgcca gatcaaagaa ggcatgcaag
    13201 ccataggctg ctaggagata aatccatttc ttctggcgaa cagcttggta aacccaaact
    13261 gtcaaaagta attggaaacc aagcgccaag attcgctcaa aaccaagcaa ataaatctgc
    13321 cagaccgaaa gtgactgaat ggtttttaac atattttcag acagtaattg cataacctgt
    13381 ggattctgag tttgaactgc cgaaagaacg atgtaaagat tgagtaaact aataaggcct
    13441 agaaaaatca actccaagcc accatgccct aggccatagg ccaaggcatc tgccttttcc
    13501 aaacttctct ttttctccaa ccatttgaag aaaacaagac gagccgtttc ctcaaaaaat
    13561 gctgccatgg ctaggccata gataatatag ataagcggat gatcttgcaa gagggcaata
    13621 ctaccgtctt tttgaggatg caaaatcaag atatgcacca gtttttccaa aatctgcgaa
    13681 gagacaaaga aagcgactgc acccaagccc aagacagcta gattaatctg gtatttcttt
    13741 ttggcaaacc aaatgcttcc tatcagaaaa gctagcaaca gcaacatggt aataatgata
    13801 tgaatggtca ttttcttctc ctattctgcc ttttcaatat cttttttcat ctcgtcaaca
    13861 ttgaacttag caaacaagta ttgacggtct tggactggga aacgttggtc caactggtca
    13921 actgctccca cctcgataat gccttccttg atgacaaaat ccatgacatg tccaccgaag
    13981 gtcaaatcat ctgatatgaa gtgcagatgg tagcctgcca cactgacccc atggaaaatc
    14041 tcaggcgtcc agaaaccaac aatagtcccc gcaacattgt cacgactata ttccggttga
    14101 tgggttgcga catcagcaaa cttggtatcg ggtgttgact taggaatcat acgcacatgc
    14161 atatgtgaaa attccccccg aatcttgata gagcggaaaa gattttcccc atcataataa
    14221 gactcaattc gttctttcaa ttccttgtct gtcatctcaa agcgctggcg gaaaatgacc
    14281 tctgcctgat gcggtaccac tgcagcgtaa ggaataaggg catctggtga cacttccaca
    14341 atttctggct ggtctcctga ccctttggcc tgataagcct tgccatccaa gacaatcaat
    14401 tccccatcaa tggaatccaa ggttcccaaa ccaaggtcac catgttctag caattctcct
    14461 actgtcatag taccaccata aaggccagcc atcaaggctc caagggtatt gtattgaaat
    14521 aatttcactg gttcctgcac gttcttatcc attcactttc ttgtctgtta ttctataaat
    14581 cttactctta agtataccac atttgcccct agatgtgaac gagagaaacg ctctagacat
    14641 tgccaagaag gaaaaaaaag ggtacaatgt aacaaaatca agggaggtct ggaatgaaga
    14701 aacaaagcaa gtacaaagag gtcgtttcct atctgaaaaa tggtatcgag tctgaacgat
    14761 ttccgacggg tagtcgcctg ccttctatcc gtcaactgag ccttgacttt cactgcagca
    14821 aggacaccat tcaacgagcc ctgctggaat tacggcacga acaatacctc tatgccaagc
    14881 cccagagcgg ctactatgtc ctagaacaag ggcaacatca agacctagaa atcgaggtta
    14941 ccgacgaaca tgccagtgcc tatgacgatt tccgactctg tgtcaatgaa accttgattg
    15001 gacgagaaaa ctacctcttc aactactatg acaatcaaga aggattagaa gacctaagac
    15061 agtccattca caaactccta tttgagcaag ctctctactg caaggctaac caactagtac
    15121 tgacttctgg aacccaacaa gccttattta tcctctctca aatatccttt cctagacaag
    15181 ccaaggaaat cttggtggaa cagccaacct accatcggat gaatcgcctc ttgattgcac
    15241 aggggctgga ctatcaaacg attgaacgag gcattgatgg gattgacttg gaggagctgg
    15301 aaggccactt caaaacagga aaaattaaat ttttctacac cattcctaga tttcactatc
    15361 ctctgggaca ttcctattct gagcaagaca aacgatctat tcttaactta gctgccaagt
    15421 atgatgtcta tatcgtagag gacgattatc tgggtgattt ggactccaag aagggtcaaa
    15481 ccttccacta tcttgataca gaggagcatg tcatttatat caagtccttc tcgaccagcc
    15541 tttttcctgc ccttcgtatt acagcactca ttcttccaaa tgctatcaaa gaagcatttg
    15601 tggcctacaa aaatatccta gactacgaca gcaacctcat tatgcaaaag gccctgtcac
    15661 tctatattga cagtcaattg tttgaaaaaa atcgtttggc tcgcttgacc aatcatgaat
    15721 cttaccaaaa acaaatcgag gaaaggataa ctaaaacacc ttgtcccctt cctcattatc
    15781 ccctacacga tggtttattg ctagacctga gacagtatcc taaaatcgcc agtctcaaac
    15841 acagtcaact gggcttggac ttctttgaag aggcctattt aagcacctgt ccttatcaat
    15901 ttgccaaggt gtccctagac aatctggaaa atgttttaaa ctatttaaaa gcagaattgg
    15961 aatgacatcc aactctgctt tttcttattc ttcaacttca gttagacttg ctgctttttc
    16021 aagatagctt tggtaagttc cttcatcctt aagtttttgg atgaccttgt ctaccacttc
    16081 tttcaaatca tcactatttt ttctaagtgc aacggcattg gcgtcgccgt ccttcatctt
    16141 caagctgaca gttgcgacag ctaagccagc gtttttagca gcataactaa gtgcaacagg
    16201 ctcatccata tgaacagcat ctatttttcc agcctgcaat tcattgactg cttcacccat
    16261 attagttagg gaagttaatt gagcttttgg caattgttcc ttgaccattg attctggaac
    16321 agtccctttt tgggctgcaa tattagcact ttctaggcta gttaaatcct tgtatttttc
    16381 cacatcagcc ttacgaacca agaaactaat cttgttttca tagtatggga ttgaaaaatc
    16441 aaagacttct tttctctcgt cagtagcact aattcctgca actgctaggt cagccttacc
    16501 agtttgaaga ctggtcaaaa cattgtcaaa actcatgctt gagatttcca acttaacccc
    16561 aagttcatca gcgatagcct gagccatgtc gatgtctgca ccgactacct ggttctttcc
    16621 atcaaccaat gattgaaatt caaagggtgc atagtcagga ctggttgcca caactaattt
    16681 ccccttttgc ttaatggcct caacagctga ctgagaacca ttagaacctg actggcaagc
    16741 tactaagaag aaacttgcaa gaaaattaca taaaacaaat atccattttt ttgatttcat
    16801 aaataaacaa cctctctgtt aattttgtat aattataacg atatccaagt tacttgtcaa
    16861 gtgtttttta aatttttatc tcaaaaatat tttttcgttc aaaaaaagga gccatcagtt
    16921 gatttcaagc tcctttttat acagaattaa actattttat agttcgacaa tcttacctgt
    16981 ttcaaagtag acaacccatt cacagatatt tttagcatag tcaccgatac gctccaagta
    17041 ggaaataact tggaaataat cacgacccgt aacaatggct tctggatttt tcttaatctc
    17101 ttcagtcgca aggtcacgga tagtttcaaa atagtggtta atttgctcat ccatggaggc
    17161 cacccggtat gcgtcgtcaa cagaaccatt aagataaaga tcaagtgctg cttcaacaac
    17221 gcttttaact tcacgtccca tttttttaat ttcttcctct acagctggaa tacgttcttc
    17281 ccccttcata cggatggttg cctgggcaat ggctacagcg tgatccccca tacgctccac
    17341 atctgataca gccttaagga cagtcaagac tgtacgcaaa tcttgagaga ctggttgttg
    17401 gagtgcgatc atttcaaatg atttcttttc cagtttcact tcgtattcat ttacttctgc
    17461 atcatcttcg atgacctctt ttgccaggtc acggtcatgc gtgacaaaag cacgtaccgt
    17521 acgattgatt tgtgagagca cttcttgtcc catagcgtag aactggttat gtaatttctc
    17581 taaatcttct tcaaattgag atcgtaacat ctttcatctc cttatccaaa ttttcctgta
    17641 atatagtctt ccgtttcctt gtgttgggga tcaaggaaca tctgcttggt atcattaaat
    17701 tcaatcaaat ctccatctag gaaaaatcct gtcttatcag agatacgtga agcttgctgc
    17761 atggaacggg ttaccagaag catggtgtac ttgtctttta gaccatacaa ggtttcctca
    17821 attttaccag ctgaaatcgg atccaaagcc gaagttggct catccaagag gatgatttta
    17881 ggactagttg ccaagacacg ggccacgcag acacgctgct gttgaccacc tgacaatcca
    17941 atagctgaat catatagacg atccttgacc tcatcccaga tagaggcacc ttgcaaggct
    18001 ttttctacgg cttcatccag aacctgctta tccttaattc cattgatacg aagcccgtag
    18061 acaacattct catagatagt catagggaaa ggattaggtt gttggaaaac cattccgatt
    18121 tccttacgta attcaaccgt atctgtacgc ggactgtaga tgttgtgacc attgtacacc
    18181 acggatccag ttgtggtcac ctctggattg agatctccca tgcggttgag agacttgagg
    18241 agggttgact tccctgatcc agatggacca atcaaggctg taatttcctt aggttggaaa
    18301 gatagggaaa cactattcaa agccttcttt ttattataat aaacggacag gtctgatacc
    18361 tgtaaaatcg catctgtcat acggtttcct ttctaaccaa agtgaccaga tacatagtca
    18421 ttggtggact gtagcttggc attttggaaa atagttgcag tcttgtcata ctcaatcaaa
    18481 tcacccaagt aaaagaagcc tgtatagtca cttgcacgag cagcctgctg catattatgc
    18541 gttacaatga tgatggtaaa gtttttcttg agctcaaaca tggtctcttc tagttgcatg
    18601 gtcgcaatcg gatccaaggc tgaggctggc tcatccatta agaggatatc tggcttaaca
    18661 gagatggcac gagcgataca gagacgttgt tgctgaccac ctgataaggt caaggctgac
    18721 ttgtggagat cgtctttaac ctgatcccag agggcagcct gacgaaggga ggtttctacg
    18781 atttcatcta ggacttgctt atccttaact ccagcacgtt catgcgcaaa ggtaatatta
    18841 cggtaaattg acttagcaaa tggattgggg cgttgaaaaa ccattccaat gtgtttacgc
    18901 atttcataaa cgttgatttc tggacggttg acatcaattc cacgatagag aatctgccca
    18961 gttactttag caatatcaat agtatcattc atgcgattga gactgcgtaa gtaggtagat
    19021 ttccccgatc ccgacgggcc aatcaaagct gtaattttat ttctttcaaa ttgcatatca
    19081 atccccttaa tggattcatt tttaccatag taaacatgga catccttagt agaaagggct
    19141 actttttctt caggaaaggt aaggatatgc ttctcatccc agttatatgt tgacatggct
    19201 tctcctttag gcagcggtta atttcttgtg tagatagctt ccgaacttac gagctccaaa
    19261 gttaaaaatc aggataaaga tcaggagcac agcggcagaa cctgctgata caatggttcc
    19321 atctggaata gtgccttcac tattgacttt ccagatatgg acagccaagg tttctgcttg
    19381 acggaagata gagatggggc tagtcacact gaggatattc cagttagacc agtcaagagc
    19441 tggcgccgat tgccctgctg tatagatcag agctgcagct tcgccaaaga tacgaccaga
    19501 tgccaagacg acacccgtta caatacctgg aagcgcttct ggaataacaa catgaaccac
    19561 tgtctcccag cgagaaatcc caagagccag accagcctca cgttgggtat ggtgaacgtg
    19621 tttcaaacta tcttctacat tacgcgtcat ctgaggcaag ttaaagactg tcaaggccaa
    19681 ggcacctgaa atgattgaaa atccatactc aaactggact acaaagatca agtaaccaaa
    19741 gagacccacc accactgatg gtaaagagga caagatttca atacaagtcc gcacaaagtt
    19801 ggtaacagga ccttttttag catattcagc caagtaaatc ccagctccca tagaaagagg
    19861 tacagaaata atcaaggtaa tgaccaatag gaaaaaggaa ttgtaaagct gaatgccaat
    19921 tccaccacct gcttgaaaag cagaagacct tccagtcaag aaagaccaag agatatgggg
    19981 caagccccga accaagatat agagaatcaa ggaagccaag attgtcacaa tgatgctagc
    20041 aatcgtatag aggacagctg ttgcaagttt atctaatttc ttagcgcgca taatttttct
    20101 ttcctctttc tttcgtaatc aatttaatca cactgttaaa aactaaactc atcaagagca
    20161 gtaccaaggc cagtgaccag agaacattat tatttacagt tcccatgaca gtgttcccaa
    20221 ttcccatagt taatatagaa gttaaagttg cagctggtgt ggtcaaggaa gttgggataa
    20281 cagctgagtt tccgacaacc atctggatag ctaaagcctc accaaaggca cgcgccatcc
    20341 caaagaccac tgcagtgaaa ataccagaac gggccgcctt caagatcaca cgccagatag
    20401 tctgccagcg agtggctccc atagcgaaac tggcttcacg ataataacga ggaaccgcac
    20461 gcaagctatc cgttgtcata aaggttacgg tcggcaaaat catgacaaag aggacggaaa
    20521 tccctgacaa aatcccaaaa ccagtcccac caaagacact gcgaacaaag ggaacgacga
    20581 cttgcaagcc aataaatccg tacactactg aaggaatccc aaccaggagt tcaatagctg
    20641 gttgcaaaat cttcgcccct tttggtgata cttcggtcat aaaaactgct gcaccaatag
    20701 caaagggggt tgcgataagg gctgagagaa tggtaacgat aaaggaaccc aaaatcatag
    20761 gaagggcacc aaattcttta ctagaaggat tccaagttcc tcccaaaaga aagtcaaaga
    20821 tattcacacc attgacaaag aaggtcgaca agcctttttg cgctacgaaa accaaaatca
    20881 tggccacaag gatgactatc aaagaaagac aggcaaaggt caaacctttt cctaatttct
    20941 ccagacgaga attctttgat ggaagcaaca ttttcttagc taattcttct tgattcatta
    21001 ttgtctccct tccaacactg tcacagttcc ggcagcatct ttttcaacct tcatttcctt
    21061 aatcggaata tacttcaatc ctttgacaat cccttcttgg gtctcatccg agagaacaaa
    21121 attgagaaat tctgcagcca actcattggg ctgccccaat gtatacatat gctcataaga
    21181 ccacaagggc caattattgc tacttatatt ttctggactt aagtcatagc cattcaactt
    21241 catgcttttg accgaatcat ctatataggt aagagataaa taagagatag ctcctggact
    21301 ttttgatacg attgatttta ccgctccatt tgaatcctgc tcctgacttt gcatggcaga
    21361 ctgaccttcc ataatgacag tatcaaaggt agcacgagaa ccagagccgg ctgcccgatt
    21421 gataacagag atgggtaagt ccttaccacc aacctctttc caattggtta cctcacctat
    21481 gaagatttga cgaagttgct ctgtcgttag gttatcaaca tcaacctcct tattgacaat
    21541 cagagccaag ccagctaccg cgaccttgtg gtcaacaaga gcagaagcat caattccgtc
    21601 tttttcctca gcaaatacat ctgagtttcc tatatcaact gccccagact gaacctggga
    21661 caagcctgta ccagaacctc ccccttggac attgaccgtt tttccaacat ggatcgtgcc
    21721 aaattcatct gccgctactt caaccaaggg ttgcaaggca gttgagccaa cagccgttat
    21781 ggattctcca cgatcaatcc agctagcaca gcctactaaa caagccgtca gccaaaaagc
    21841 gataagagac agagcaagct tttttctttt tttcactgtt tttctcctcg aaaataatta
    21901 tgaatactgt gaatttttta agtagttctt tatgagttga cgcatgaatt cttaccaaat
    21961 ttctgcgcaa ttgattattt atataatata ggctatatta ttctttccta acctcctttt
    22021 ttcatatgtg gataaaatct cttgtctatc ccttccccca ttgtcaccca ttatagtcat
    22081 ttcgtgtctc tttttcccct ttttaatgca agggaaatta ctctccttag atgataatcc
    22141 aaaagctaga aaggtatctc aaacctctct actctcccag actagtttac aactaaaaga
    22201 aaaagattct attttatgag aaatctagtt tacaagcggt aagaacgcta ataactaaac
    22261 ttcttgtact ctttgaaaat ctcttcaaac cagtgttttg agctatctat ggctagcttc
    22321 ctagtttgct ctttgatttt catcgagtag taaaactaca tgtgatggca atcaagatat
    22381 caagaatcat cctactaaaa aaatccatac tttcactata acatagaata agatatttga
    22441 ctagcatttt catttgaatc tgaggccttt tggaaaataa tttttcaaaa catttccagt
    22501 aacctttgca aagcccaagc cattgccttt aaccaaaact tggtaccaac catttggcag
    22561 actttctgcc agctgaacgg tttctccagc cgcatacttg acaaacgctt cttggccaat
    22621 ttcaaccgac tgttcgacct gactcggttt caaggctaaa ccaagagcga aactgggctc
    22681 aaagcgtttc ttcttaaaag tacccagatg cagtccattg cgagcaatct tgagcttccc
    22741 taaatctggc aaaagttctg gcaagagata aagctggtct ccaaaaatct gcaagatacc
    22801 cggtagattg accttcaaat ggttttgggc aaattcctgc cacaaggcaa cttgttcacg
    22861 gctgaggtta ctcttacttg ccttaaattt aggagctgga ttgttaccct taaactgtag
    22921 atgggcaaca aactgaccct ctcccttaaa ctgatgaggg tacatccgag ccgtttctgg
    22981 caggtcaata ccagctacca ttccattgat atgctctact ggcaacaagt caaaatcata
    23041 ctcttccagc aaccaattga caatctcttc gttttcctcg ggtgcccagg tacaggtcga
    23101 ataaaccaga cgaccacctt cagctaacat ggtcactgca tcctccagaa tttctctttg
    23161 caagctagca cattgactcg gataatctaa gctccaatag tccatagcat caggttgctt
    23221 acgaaacatt ccttcaccag agcaaggggc atcaagaacg attaagtcaa aatagccttt
    23281 aaagaccttg accaagcggt cggcagattc attggtcacc acgacatttg tcgctccaaa
    23341 acgctccatg ttttcaacca aaatcttagc ccgtttgctt gaaatttcat tggaaacaag
    23401 tagcccctcc cctgctagat aggctgccag ttgagttgat ttgccccccg gtgcagcagc
    23461 caagtccaag accttcatac caggactggg ttgggctact tgagccacca tttgagcagc
    23521 aggttcttgc gaataaacta aacctgtagc atgctcaggc gatttccctg aaaccttccc
    23581 atagtggccc caaggggttt gagtaatggc atcagaaaag gaaagttgct cttcttttaa
    23641 gggattgacc cgaaaggccg aaaccgcttc ctcctcaaaa gaggcaagaa aatctcttgc
    23701 ctcatctcct agtatctctt tatatttttc aacaaatcct tctggaaatt gcatttaagt
    23761 tcttttcctt tcgtaaatat aggactgaat ttcctcctgc atctcaagag gcaccatcat
    23821 gaccggctgt ctggtttgaa aatcaggagc ttcaccaaaa agggtcacaa cccgatagcc
    23881 cagactttcc cctaaaatac tagctgcggc ataatcccat ggttgcagat aagtgagata
    23941 ggtcaacaaa cgccctgaca aaatcttggc aaaactaatg gccgcacttc catagacacg
    24001 aacaccaaga accgctcggc tcaaatcagc cagcccccat tcattggttt ccagcatacc
    24061 actattccct gcaatgagaa aatctccaag tggtttagtt ttaaaaggag ctaggggcct
    24121 atcatttaga caaactggaa attccccacc accgtggtaa caatcccctt tgaccacatc
    24181 ataaatcaga ccaaactgtc cctgaccatt ttcaaaataa gccatcataa cagcaaaatc
    24241 ttcctgctgg gctacaaaat tattggtacc atcaatggga tcaatgaccc aaaccttgcc
    24301 ctcttgaacc gaggctcgca gacaaccttc ttcagcacaa atcttatcct caggataacg
    24361 ggacaaaatc tcaccaacca agagttcctg aacttctttg tccagtctgg tcaccaaatc
    24421 tgttggagaa gacttggttt caacacgcaa gtcttcctgc atatggtcaa gaatgtactg
    24481 acctgctttc ttaacaagct ctttagcaaa ttcaaattta ctttccaaga gaaatctttc
    24541 cttccccttt ttctttggca gcctgaactg ctcggtaaag tgaatagcca ctaaccgttt
    24601 caaattctcg tcccaaacgt ttctcttcgc tcttgcttgg cacgaccgct ttgaatccct
    24661 tataggagtc cagtaacttt ttagcctcta ctttgccttc ataggcagct tcaacatcat
    24721 taaaaaaaga aagcactgaa gcaagttctt cagtgctcca cgacaaatct agtgggtaac
    24781 tatactgttt gttcattaac taataccagc tctcattctt gcttctttta gttcttgctt
    24841 acgataacta cgagggagaa aagcacgaat ctcatcttca ttaaaaccga tttgcatacg
    24901 cttggcatca ataataattg gacgacgcaa aagactagga tactgctcaa tcaaatgaag
    24961 caattccgat accgaaatac tctctacatc aatattcaat ttttgaaaaa tttttgaacg
    25021 agttgaaatg atgtcatcag taccattttc ggtcaaggaa aggatgtgtt gcaattcttt
    25081 tcttgttaaa ggactggtca taatattgtg ttccacaaag ggaaccttat gtttttctaa
    25141 ccaggcctta gccttacgac atgatgtaca gctcggtgat agaaatagtg taatcatgct
    25201 tttctcttct tatctatact ttgctacttc tattatacaa aaaaataaag cgcttgacta
    25261 gggattttta gaaaaaaagc ctattttttc aagaaaaata ggctgtttgc gaacgattga
    25321 cacaattgga tttggttaat tcactcttaa cgatggtttt aaacgatata tatttttata
    25381 tatgcaaatt aaaaacatct ttcatttcac ttcctacgac ttttcagaca cagatagcca
    25441 aagaagcttt catagagggc aaaaaagagg aggaaggcat gaagaaagaa ggtctctggc
    25501 aaaatcataa taacaggatc cttggctgga tcaaaaagcc aggtatcatc tcccacaaag
    25561 agaatttgat ggaaaagagt aaagaattgg tcaaaaccaa tcaaaactcc cccaagtcca
    25621 atcatcacag gtaagactac tagagtcagg agactttttc gataaagaga caaaaagtcc
    25681 tttttcacaa tcctattgac aaagacatag aaacttggca gtgtcactag agctactagc
    25741 tgagccaaat gaaagagatt cttgaccact gcgaaatggt gcagaccagc tgctgacgaa
    25801 cgaaaatcag gcatctgtaa gacctgacta aaaggattgg tcagataatt cattaagata
    25861 tgaaaattgt attgaatggt ttctggtttt agatagactc gatttgttaa gtttagccac
    25921 tgaatctcca taggatagaa aatccaagcc agataaatgg tcagaaggat tgagagggag
    25981 aggagaaaga gcatagagcc ccaaaaggtc aatttagttt tcatcaaaat cccactccgc
    26041 aaggctagaa accacatgtg tcggtgcgat tggcaggcca gctacttctt ctgccttagt
    26101 aaaacctgtc gtcaccaaga gcgttggaat gccattgtca atcccagccc ggatatcagt
    26161 caaataattg tccccaacca tgattaactc ttcacgttcc aaacctaagt gctcaaccgc
    26221 cttgtccata atgatggcat tgggttttcc gatataaacc ggcttcactc gtgtcgctac
    26281 ttcaagcagc gtaatcagtg agccagcacc tggcaaaaga ccgcgttccg tcgggatgtt
    26341 gaggtcagga ttggttccga taaaatgggc acccttttga atagcaagag ttgctgtggc
    26401 aaatttttca tagtcgactt gccaatccag accaactacc acgtaggcag ggttttcctt
    26461 gtcttccaca taaccagccg ccttgatggc ttccttgagt cctgcttctc cgacgacata
    26521 gacggtcttt tcaagcccca aatcattcat atagtcgatg gttgccaaag tcgctgtgta
    26581 gacagtcgat aggggcgtat cgatattaaa attctgagcc aacatctcct taacactctc
    26641 tggagtgcga gttgtattgt tggttacaaa gagataggga atgccccgct tttgcaattc
    26701 atgaacaaaa gtctctccag cagggattcg gtctttcccc ttataaatgg ttccgtctaa
    26761 atcaattaaa tagcctttat atttcatcta tttctcccta agcctttttt atttcttgcc
    26821 aagtaatgat tgcttgggca ttgataaccc catcacttgt aatttcatgc ttgctttcca
    26881 gtccagtccg ttcaacaacc gatgtaatca ctccacctgg tcgaacttcc ttgacatact
    26941 tgaggttgat tttcttggga atatagtggg tcaaaaaatc cgctcccatg acctcaaaaa
    27001 tccagtccaa gtatttactg ttattgacat gaccattcat atccaagtcg taaaaacgaa
    27061 catggtaatc cttgctgatc ggttcttcca aggactcata cttcggtcca cggataagtt
    27121 ttttatcaaa atcagactgg taaggagcca caatctcagg ttcaacagca tggacttttc
    27181 gactgtcgcg gtccatgaga acaaaggtcg ccatcatatg gatgagctcc tgccccgctt
    27241 cattataaat agtaaagcga cggtagcaaa aaagtcgatt gtagctcaag gcttccgttt
    27301 cgatggtaat ttcttccgca aaacgaggca aacgaaccac ctcaatatca tattctgcga
    27361 taatccagac cagattatag tcttccaaaa tggccttatc actaactccc agttcaatcg
    27421 attgcatccc tgaaacttgc agtgacagca aaatcacatc tggaagtttg atatgaccgt
    27481 tcatatcagc catatcaaaa ggaattttca ttttcatttg gtaagttaag cccatgatcc
    27541 tactccaaaa taaatcgttc tgctacagta tctcccaaaa agagacctct ctttgtcatg
    27601 cgaacgtggt caccctcaat ctgcatgagg ccttgttgaa ccaaatctct gacaatttct
    27661 ccataaagtc cagcaaaaga ctgtccaaat ttttcctcaa atcgcgccat ggaaaccccg
    27721 gatttcttgc ggagtcccaa gaacatttct tcttccattt gctccttttg actcaggtga
    27781 tcttctgtaa tacaagcatt gccttcctca accgcactga gataatgacg aatgggacca
    27841 tgatttttat agcgtactcc attgacataa ccagatgccc ctgcaccaat accatagtat
    27901 tcagcattgt cccagtacat gagattgtga cgactttcaa agccgggttt ggagaaatta
    27961 gaaatctcat aatgctcaaa acccgctcgc tccagctctg caatgatgta ctcaaacatc
    28021 tccgcttcta gttcctcctt aggcagaggc aatttcccac gtcgcatccg gttcataaag
    28081 accgtatggt tttctaaaat caaactatac aaactcatgt ggggaatatc cagtccaatg
    28141 gctttagcca cattttcctt tacttgctcc atggtctgac caggcagagc ataaatcaaa
    28201 tcaatggaga tgttgtcaaa accagccagt ttcaggcgat cgatattttc ataaatatcc
    28261 ttctccaaat gactgcgccc aatctttttc aacatcttat catcaaaggt ctggacacct
    28321 agcgaaacac gattgacagc cgaatttttc aaaacagcta tcttatccgc atccaaatcg
    28381 cctggattgg cttcaatggt caactcttct aagacagaca agtccaagtt tttagtcaag
    28441 ccattcagta aaacctccag ttgcggagcc gacagggctg tcggtgttcc accaccgata
    28501 taaagggttg acaacttttc aatatcataa gaacgaaact cttccagcag atgctctaaa
    28561 tagctgtcga ctggctgatt tttgatgaag acctttgaaa aatcacaata ataacaaatc
    28621 tgggtacaaa atgggatgtg cacataggct gacgttggtt ttttctgcat agtaattatt
    28681 ataccacaaa gactagattc cagataaaaa tcaccatccc cagatacata gtccgttcgg
    28741 agatggtgat ggtttattct tctgttatat caatcacaat ctcttctgag tcatcaagag
    28801 cttcggcttt ttcttgccat tgttccttga gattatttaa ttgatttttt gatgcttctg
    28861 tcgcttgaaa agcataggat ttagcttgag caagtatact gtccacagtg atttcacctg
    28921 actcaacctg ttcttttgtt ttcagaacaa aatctgtagc ctgctcctta acttctgtca
    28981 gtttttcaca gacttgctcc ttggcatact ccggatcttc tctcaaatca tctagaaaat
    29041 cttgagcctg actgcaaatt tgtttgccct tatcacttgt taaaaacaag gcaagagctg
    29101 cacctgaaac cgttcctaaa aggattgagg ataatttacc cataaggatt ctcctttttt
    29161 attttttgaa aaatttactt gcaagacgaa gagctgacag acttgcacca gtcttgagtg
    29221 tttttgaacc agctgatgaa gctttcttgc tcaagacacg cgcatggtca ttgaggtctg
    29281 aaacagatag agataaatct gcaacagcac tgaagagtgg atcaatcgta gccaccttga
    29341 cattgatatc atctgccaag acattgacct tagccaacaa ctcattggtg tgatgcaagg
    29401 tcacatccac atctgaagtc aaggttttaa tcgtcttttc tgtttcatcg atgacacgac
    29461 caagcttttg tacagtaatg atcagataga ccaaaaagac aatcaaagct agggcaacaa
    29521 gaatatatgc aacttctaac atttagtttt cctcctctgt aatatagtaa ggggccttct
    29581 ttcgattttg ataaataacg atcattatac cgagaccgat aaggacaact gacagccatt
    29641 gggacactcg aaagccgaag aacatgagac tatctgttcg cataccttcg ataaccatac
    29701 gaccgaaacc ataccaaatc aagtaaaagg ccgtgatatg acctcgtctg agactcttcc
    29761 atttccgtct aaaaatcaga atcaaggcaa agccaagcag attccataga gactcataaa
    29821 ggaaagtcgg ttgacggtag ctcccctcaa tatacatctg gtcacggata aagccaggta
    29881 gataatccag attatccact gttgcaccat aagcttcttg gttaaagaaa ttaccccaac
    29941 gccccaaact ttgagcaatc ataacgctag gcgccgcaat atctagaaaa tcccaagtat
    30001 tgatgagttt acggtcagca aagatataga gcacaagagc cccagttatc aaaccaccgt
    30061 aaatggccaa accaccattc caaatggcaa aaatctctcc taaattctga ctatagtaat
    30121 caaatcggaa aataacatag tagagacgag ctcctaaaat agccaaggga aaggctacta
    30181 agataaaatc taaaatatcg tctggtatga tcttctttct aggtgcttct ttcatggtca
    30241 aataaaccgc aagaatcaag cctgtcacaa tacataaggc ataccaacga atggctaggg
    30301 gtcctagttg aatagcaatt ggatcaagca ttttgcacct catttcgagc gattagactt
    30361 gtcagtcgtt cgtcgaacaa acgggtcgca tcaaagccca tttccttggc acgataattc
    30421 atggcagctg cctcaatcac aacagagata ttacgacctg ttttaactgg aatacgaata
    30481 cgaggaatgg ctacgccaga aacttcaagt tcctctgcat tgtttccaag acgatcaaag
    30541 gtcttatgcg tatcgtaatt ttccaaatag acagcaagct gaacctgtga agaatccttg
    30601 acagcactcg caccgtagag actcataaca tcgataatac caaccccacg aatttcaatc
    30661 aagtgtttca aaatttcagc tggttcaccc cagagagtaa tctcatcctt ggcaaagata
    30721 tcgacacggt catcggctac caaacggtga ccacgtttga caagctcaag acctgtctcg
    30781 ctcttaccaa ttccactatc tccctgaatc aagacgccca tcccataaat atccatcaag
    30841 acaccgtgca cactggtacg ttctgccaaa cgagaatcca gatagctaga taactctcca
    30901 gataaacgac tggtagctgc acggctggtt aaaatagcaa tcttacattc tctagcagcc
    30961 tttaacatct cctctggaac caccaaacca cgggcaacaa tgactgctgg tgtctcaggt
    31021 agaaacattt ttttcaaaac ttcataacgg ctgttggaag gcatgctgat cagataagac
    31081 cactccttca tccccaaaag ttgaatccgc tctggtgtat agtagtcaaa atagcctgtc
    31141 atttcaagac caggtcgcgt aatatccgct gtattgattt ccttttcaag caattctggt
    31201 tcaccataga caatgtctag tctaagcttt tcaatcactt cttttactaa aaccgacatc
    31261 atttcctcct tcaaatgagt tcttctctct attatatcaa attgtaggag gaagtttcag
    31321 atttttgcag gctttggagt atttatttaa aagaaaaagg actagatttc tctagccttt
    31381 catttcaatt agaatttttt acgtttacga gctacttctg atttacgttt acgttttaca
    31441 gaaggttttt catagaattc acgtttacgt gtttcttgaa gagtaccagc tttagtaacc
    31501 gcacgtttga aacggcgaag tgcgtcgtca agagattcat tcttacgtac tactgtttta
    31561 gacatttttt tcactccctt caagttcaag atctattcta ttttacacgt aaaaggaata
    31621 attgtcaaga ggaaactaag ttttttattc ttcataatga ttaataatat agttcaccat
    31681 ctcctccata tctgcttcag ataaaggtaa ttcccagttt gttgtttcca ttttaagaaa
    31741 agcatcactg actttagtta ttgtatagca atctttaaaa attggaattt ttttagattg
    31801 ttcaaataag atgcctattc tatcatataa gacgtgaagt tctttactat ttggatcaat
    31861 atcaaaagac tgtaaagaga ttaagatgtc atttccttta tatgacaaat caatccacat
    31921 gtgatttgat aaatcatatg gcctagataa tctactaccc gaggtgtact ttttatttcc
    31981 tccgcctcct gaatattgat ttcttgtggt atatggttta tcttttgact tagtaaaaag
    32041 aaaattcccc tccttatctt tcttttttgt aatgaagcct attatacttt ttctttcttc
    32101 cgttctagca ctcttatatg cttcttttat ttttaaaaat tcttctttat tcatgtgtcc
    32161 tcctaaagtt aagttgaagt tttatatgtc taaaaccact cactccaaag agcaagcggt
    32221 tcttatactt tttatttttc gagtaagcta agcgcttcag catctgcgat gatggttaca
    32281 tcagggtgat tttggaggct acttgcaggt aggttctcag tcactgggcc agatactgtt
    32341 ccggcaatgg cttctgcttt cgactcaccg taagcaaaaa gaataataga cttggcatcc
    32401 aaaatgtttt taatccccat tgaaatagct tgggttggga cgtcttcaat cttggcaaag
    32461 aagcgtgcat tggcttcgat agtagactgg tcaagttcta ctacatgcgt ttgactgtca
    32521 aatggagtac caggctcatt aaagccgata tgcccattgc gaccgattcc caagatttgg
    32581 aggtcaactg gatggtcagc caaaatttgg ttgtagcgtt caacttcagc ttcagcattg
    32641 tccttaacac cacgaggcaa gaaactttct ttaaatggtt tttggttgaa caagttttct
    32701 tgcatgaagt aacgataaga ctgtggattg tccccatcaa ggcccacata ctcatcaagg
    32761 ttgacactgg ttagatttga aaaataaagg tcactctcaa caatttcctt gtaaaattca
    32821 agtgggctac ttcctgtcgc aagtcctaga gtttgagctc cattagccaa tttttccttc
    32881 aaaatctcaa aagcaacttt tccaccttga acttggtttt caactttaat aactttcatc
    32941 ttttcatcct ccatttctgt ctatatttat tatatggtat agaccaattt ttgtcaagtg
    33001 aaaacgattt aatttctaaa aaaagagcgt ccaaacttga aacgtgttat aatggatagg
    33061 attagaactt agaaagaatg aacaaatata tgaatacagc tgattttgat ttccacttgc
    33121 cagaggaatt gattgcccaa acgccccttg aaaaacgtga tgcctccaaa ctcctcatcg
    33181 tcaaccgtga gacaggagaa atgcaagata aacatttcca ctctattatt gatatgctgg
    33241 aacctggtga tgcccttgtc atgaacgaca cccgagttct ccctgcccgc ctctatggtc
    33301 aaaaagtgga gacaggaggt catgtggaac ttctcctcct taagaacact agtggagacg
    33361 agtgggaagt tctggctaaa cctgccaaac gcctcaaggt cggtactcgt atcagctttg
    33421 gtgatggccg cctcagcgct gtcgttacag aagaattgac ccacggggga cgcattgtcc
    33481 gctttgaata ccaaggaatt ttcctagaag tcttggaaag tctgggagaa atgcctctgc
    33541 caccttatat ccacgaaaaa ttagatgacc gtgaacgtta tcaaaccgtc tacgccaagg
    33601 aaagcggctc tgctgcagca ccgactgctg gtcttcactt caccaaagaa ctgctggcag
    33661 aaatccaagc taagggtgtt catctagtct atctgactct ccatgtcgga ctcggaacct
    33721 ttagacctgt ttctgtggat aatctggacg aacacgaaat gcactcagag ttctatcaac
    33781 tttctgagga agctgctgcc acccttcgct ctgtcaaaga aaatggtggt cgcgtcatcg
    33841 ctgtcggaac cacttctatc cgcaccttgg aaactattgg ttccaagttt gatgggcaaa
    33901 tccaagcaga ttctggttgg accaatatct ttatcaaacc tgggtatgag tggaaggtcg
    33961 tggatgcctt ctcaaccaac ttccatctgc caaaatcaac tctggtcatg ttggtttctg
    34021 cctttgcagg ccgtgaatta gtcttagatg cctaccacca tgccatccaa gaacactacc
    34081 gcttcttcag ttttggtgac gccatgttta tttattgaga aagaatttct ctaattcttc
    34141 taataccaat aaatcgctaa gatattattt caaagaacat ctacaattga aactctagct
    34201 agctgtagaa gacgcctagt acattgaaat taaaatgctt ccccctagct tcgaaaatat
    34261 tgccatagat tgcgttgact ctccaaattg attcatctat attttatttc agcttcctat
    34321 attttcttcg ctgtttgtaa atcaaaatgc aagacacatg agtagcacca tatttgttac
    34381 tcttatctgt cctctcaaga gactattatg agttatttca gaatcattca ctactttgac
    34441 cctgactctc cttagtctca aaatcaaaga cttatactct tcaaaaatct cttcaaaccg
    34501 cgtcaacgtc gccttggatt atatatgtga ctgacttcgt cagtcttatc tacaacctca
    34561 aagcagtgct ttgagcaacc tgcgactagt tttctagttt gctctttgat tttcattgag
    34621 tattaaacaa aaagtgaaca aatctgaatt ctaatgtaca gaagactagg cttgttcact
    34681 tttttatagt cgctataaga tgaccttatc tatagctttt tatatataat tatatattca
    34741 gacatactat tatcaatttt gtcgcaggga ggaatctgtt aacgcaccca ttcaccatta
    34801 tcattgactc tatagccatc tatacttgta ttgaccgcta actcacccga tgtatttaca
    34861 taataccatt taccaccaac ttggaaccat tgattgactt tcatagaacc gttgctgttg
    34921 aggtagtacc atgaaccatt aacttgtacc caacctgttg ccatggaacc atcagtatta
    34981 taaaaatacc acataccatt ttcttgtttc cagcctgttg ttggagcaac tgctttagct
    35041 ggttctactg ctacatctgt tccttggtta gatgtaacag atacaggata cgaaggaata
    35101 gatgattgct caggaacaac aactttttca ggttctctcg tccctctcct tatacgtctt
    35161 tttaccatct ctttagtaat ttgacgagaa gtagtttctt caattgttcc atcacgttca
    35221 tctacagtat agattgtagt aagagtaatt taccaatttc tcctacttct tctacttctt
    35281 gacttttatc aagagttggg ccatcgagat attctgtttc gattgaaatt tcttggacaa
    35341 gaacttgggg cttggttcct tttttaacaa ctcttgtttg agagtcttct ttttgactta
    35401 aagtactctc agttacttgt ccactctttc catctacatc ataagttatc gttgtaactg
    35461 ttttcccatt ctttcctaga gtaatctctt gctcctgtcc tgcagaaagg tcattgtctg
    35521 cttcatattt agtagcaaat ggaacaagaa cttcttcaac cttgctttta gctggaactt
    35581 tgataactgt atccgtggct tcttttctat caacagtaac cttttcggta acatgaccag
    35641 tctctggatt aacatcgtag gtccttgtcg tggttacata gccatcctct ccgtcaattg
    35701 taacatgatt ttcactacgg tcttttgttt catctttttc aaaacgaatt cgcgtacttg
    35761 aaattttctt ggttactacc ttaggtttag tcgctacttt tacaataata tccccattgt
    35821 cagcgtcatc atactctatt ccctcttctt tatctctagt atcatctctg acatattgaa
    35881 tcccatcagc agcatgagca aaacttgtat tcagattcct cctaaaaata aagttagccc
    35941 gattaccgca gaaccaaaaa tctttccgag tttacgtatt gcatagcgct tattagtatt
    36001 agattttgcc attacatcct acttctagta tagcatcttt tctatcaaac gttaaacaat
    36061 atacttt
//
LOCUS       DAVKGC010000007        35607 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKGC010000007 DAVKGC010000000
VERSION     DAVKGC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.007"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            202..498
                     /locus_tag="VW049_000286"
     CDS             202..498
                     /locus_tag="VW049_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9431473.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            575..2605
                     /locus_tag="VW049_000287"
     CDS             575..2605
                     /locus_tag="VW049_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9431474.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDEVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            2607..3248
                     /locus_tag="VW049_000288"
     CDS             2607..3248
                     /locus_tag="VW049_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431475.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            3241..3549
                     /locus_tag="VW049_000289"
     CDS             3241..3549
                     /locus_tag="VW049_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431476.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            3680..4552
                     /gene="rsmA"
                     /locus_tag="VW049_000290"
     CDS             3680..4552
                     /gene="rsmA"
                     /locus_tag="VW049_000290"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9431477.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILVDTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            4554..5432
                     /gene="rsgA"
                     /locus_tag="VW049_000291"
     CDS             4554..5432
                     /gene="rsgA"
                     /locus_tag="VW049_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9431478.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            5443..6099
                     /locus_tag="VW049_000292"
     CDS             5443..6099
                     /locus_tag="VW049_000292"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9431479.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            6062..6724
                     /locus_tag="VW049_000293"
     CDS             6062..6724
                     /locus_tag="VW049_000293"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9431480.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            6726..7982
                     /locus_tag="VW049_000294"
     CDS             6726..7982
                     /locus_tag="VW049_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9431481.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            7972..8913
                     /locus_tag="VW049_000295"
     CDS             7972..8913
                     /locus_tag="VW049_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9431482.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            9179..10006
                     /gene="purR"
                     /locus_tag="VW049_000296"
     CDS             9179..10006
                     /gene="purR"
                     /locus_tag="VW049_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9431483.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            10073..11323
                     /locus_tag="VW049_000297"
     CDS             10073..11323
                     /locus_tag="VW049_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9431484.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGE
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            11449..12243
                     /gene="pflA"
                     /locus_tag="VW049_000298"
     CDS             11449..12243
                     /gene="pflA"
                     /locus_tag="VW049_000298"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9431485.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(12318..13244)
                     /gene="yidC"
                     /locus_tag="VW049_000299"
     CDS             complement(12318..13244)
                     /gene="yidC"
                     /locus_tag="VW049_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9431486.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(13322..13600)
                     /locus_tag="VW049_000300"
     CDS             complement(13322..13600)
                     /locus_tag="VW049_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9431487.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            13634..14374
                     /locus_tag="VW049_000301"
     CDS             13634..14374
                     /locus_tag="VW049_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9431488.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYIELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFYLS"
     gene            14430..15113
                     /locus_tag="VW049_000302"
     CDS             14430..15113
                     /locus_tag="VW049_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9431489.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            15225..16217
                     /locus_tag="VW049_000303"
     CDS             15225..16217
                     /locus_tag="VW049_000303"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9431490.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            16282..16821
                     /gene="rsmD"
                     /locus_tag="VW049_000304"
     CDS             16282..16821
                     /gene="rsmD"
                     /locus_tag="VW049_000304"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9431491.1"
                     /translation="MKIVSGIYGRRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYTKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            16811..17299
                     /gene="coaD"
                     /locus_tag="VW049_000305"
     CDS             16811..17299
                     /gene="coaD"
                     /locus_tag="VW049_000305"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9431492.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPIENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            17283..18320
                     /locus_tag="VW049_000306"
     CDS             17283..18320
                     /locus_tag="VW049_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9431493.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            18378..18608
                     /locus_tag="VW049_000307"
     CDS             18378..18608
                     /locus_tag="VW049_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9431494.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            18632..19915
                     /gene="murA"
                     /locus_tag="VW049_000308"
     CDS             18632..19915
                     /gene="murA"
                     /locus_tag="VW049_000308"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9431495.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            19908..20096
                     /locus_tag="VW049_000309"
     CDS             19908..20096
                     /locus_tag="VW049_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9431496.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            20135..20959
                     /gene="endA"
                     /locus_tag="VW049_000310"
     CDS             20135..20959
                     /gene="endA"
                     /locus_tag="VW049_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9431497.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            21250..22581
                     /locus_tag="VW049_000311"
     CDS             21250..22581
                     /locus_tag="VW049_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9431498.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(22779..22849)
                     /locus_tag="VW049_000312"
     tRNA            complement(22779..22849)
                     /locus_tag="VW049_000312"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22815..22817),aa:Cys,seq:gca)
     gene            23265..26876
                     /gene="rpoB"
                     /locus_tag="VW049_000313"
     CDS             23265..26876
                     /gene="rpoB"
                     /locus_tag="VW049_000313"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9431499.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            26909..30586
                     /gene="rpoC"
                     /locus_tag="VW049_000314"
     CDS             26909..30586
                     /gene="rpoC"
                     /locus_tag="VW049_000314"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9431500.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            30697..31110
                     /gene="ndk"
                     /locus_tag="VW049_000315"
     CDS             30697..31110
                     /gene="ndk"
                     /locus_tag="VW049_000315"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9431501.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(32056..32688)
                     /locus_tag="VW049_000316"
     CDS             complement(32056..32688)
                     /locus_tag="VW049_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431502.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(32690..34705)
                     /locus_tag="VW049_000317"
     CDS             complement(32690..34705)
                     /locus_tag="VW049_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9431503.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKAIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEIWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            complement(34808..35104)
                     /locus_tag="VW049_000318"
     CDS             complement(34808..35104)
                     /locus_tag="VW049_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9431504.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            complement(35419..35589)
                     /locus_tag="VW049_000319"
     CDS             complement(35419..35589)
                     /locus_tag="VW049_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431505.1"
                     /translation="MKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQLEIRVQSQ
                     KKSNGLDVGKAD"
BASE COUNT        10388 a         7001 c         8068 g        10150 t
ORIGIN      
        1 aggacattga tgtccttgat agtgtgataa agatggagta taatgactat aaaagttcat
       61 ttcagggtgc tattttgaca atttagagaa aaacaaataa ttttagaata tcatttggta
      121 atataatagt atcaacgttg ataatggaaa gaggagcaag taactcaaca atcttgtaat
      181 caaaaataga ggagtatcaa tatgaaacaa acagttaaaa agttagctct tgtagcgagc
      241 attgcagcaa cattaggtgg aggtgtatca gttgcctctg cagccgttca atacccagaa
      301 ggtggtgttt ggacttatgg ttcaggtaac ggtggtgctt actcaaacta ctatcaccct
      361 tcaaaatacc atagttcaac agttgttagc agaaaaactg gttcatctga caagggatat
      421 gctggtgctg gagggacttc tcgtgcgtgg attcgtactt cttggggaga gaaagttgca
      481 ttctactata atgtttagaa tggtatagcc ctattgttta gctttacttg gtcaccttct
      541 cactaagtga ccaagtaact ttttggagga aatgatgaaa cgtttattta ttttgatttc
      601 aatggtattg gtatcgcttt atatggtgat aacttccgtt gaccatcgag aagagatttt
      661 atttggtaac tatccttctg ttgatgtgac aggaatgatg ataaatcaac cagtagctag
      721 tcgcgaagag gtgacagagg ctttgagtca cttggcggta gagcataata gtctcatcgc
      781 ccgtcgaatc gttgagccaa atgaagctgg agaaacactc tttacctatg ccacttatgg
      841 tgagggagag cttccagaag gtctgaccat ttcctccaag gagagtgcag aaacgagtga
      901 tttattaggg tcttacttga ttgtatcagg aagtttggat gaagtgagct tacagaccac
      961 cttgaaagag cttggttatc aaggctttgt ttcgaatgga gaagatccat tttcgatagt
     1021 cttactattg acggccaccc ctatggtgct actgagttta gctatttttc tgctgacctt
     1081 tatgagtctg accctgattt atcggatcaa atcccttcgt caggcaggga ttcgcttaat
     1141 agctggtgag agcttgtttg gagttgctct cagaccagtg ttagaagatg tgagacagct
     1201 tatctgctca gtgctggtat ccagtctttt gggattgggg attctctggt atcaaggtgc
     1261 cttgtttatg gcaacggtgc aactggtcat cattgctctt ctactttatg gattgacctt
     1321 ggcagggatt tctaccttac taagtgtcgt ctatctactt ggtttacagg aaaatagtct
     1381 ggtggatcta ttgaaaggga aactccctct caaacgtatg atgacattga tgatggtggg
     1441 gcaactctta gctgtattgg tggtcggatc gagtgcgaca gctctcctac cccactaccg
     1501 tgaaatgcag gaaatggaga gagctagcaa taaatggagc cagtcctcag accgttaccg
     1561 tctatccttt ggttggtcta gtgcatttgc cgatgaagaa ggaacgcgta aggataatcg
     1621 tgagtggcag acatttactg aagaacggtt agccaataca gactcttttt atattatgag
     1681 caatgttgac aatttctcag atggagcaga agtggaccta gatggcaatc gtctcagtga
     1741 ctacacaccg tcagggaatg ttatctatgt ctcaccacgc tatctgatag aagaaaagat
     1801 taccgtttct tcagagttta tggacaagat gcaaaacttg tctgagggag agtttgggct
     1861 gatcttgcct gagagcttgc gagagcagtc tgcctactac caaggattgt ttacagatta
     1921 cctgcaaaac ttttcatctg aaagtgtaga agtgacgagt cagaaacact acttcccaca
     1981 ggtaaggcta gcttttacag aaacaggaca ggaacgtttc ctctataatg atgggtacaa
     2041 gacaacacgc cagtacctaa aagatccgat tattgtagtt ctaacgccgc aagcgactgg
     2101 aacaagacct gttgcaggga tgttgtgggg aactacggct aatagtgcct tgaaactaga
     2161 tcgatatgga gacagcatca cagctctaaa agagcaaggt ctgtatcaca aggtttctta
     2221 cttggtaaaa agccagctat tttttgccaa ggtactaaat gacaaacggg tggagtttta
     2281 ctctctcctt attgggacga ttttgaccct gtctacggct atcttgttat ttgattccat
     2341 gaatcttctc tattttgagc agttcagacg ggagcttatg attaaacgtc ttgctggtat
     2401 gacaatctat gagcttcatg gcaagtattt actggcgcaa ggaggagttc tcttgcttgg
     2461 cctagtccta tctagtattt tgacaagaga tggtttgatt agcgctctag ttgtagcttt
     2521 gtttacgctt aacgccctct tgattttagt aaggcaggac aaaaaagaag aagctggtag
     2581 catggcagta ttgaaaggaa aataagatga ttgatattca aggattggaa aagaaattta
     2641 atgaccgcgc gattttctct ggtttgaatc tcaagctgga gaagggcaag gtttatgcct
     2701 taatcggaaa gagtggaagc ggaaagacga cgctgctgaa tatcttggga aagctagaaa
     2761 agatagatgg aggaagggtt ctctatcagg ggaaagattt aaaaaccatt cccactcgtg
     2821 agtattttcg agatcagatg ggctatctct ttcaaaattt tggcctctta gaaaaccaat
     2881 caatcaaaga aaatttggat ttgggttttg ttggtcagaa aatctcaaaa gtagaacgtt
     2941 tggaaaggca agtgggggct ttagaaaaag ttaatctagg gtatttggat ttagaacaaa
     3001 aaatttatac tttatctggg ggagaggccc aacgagttgc ccttgctaag actattttga
     3061 aaaatccacc cttgattttg gcagatgaac caacagcagc tcttgatcct gaaaattcag
     3121 aggaggttat gaatctcttg gtggatttga aagatgaaaa tcgaattatc atcattgcga
     3181 cccataatcc cctagtctgg aataaggctg atgaaatcat tgatatgagg aaacttgctc
     3241 atgtgtgaaa aaatccgtat tcgcagggta tctgattatc ctagtgccag aggtggttta
     3301 gaagatatcc tcatcatgga aaatatgacc aatcatctcc ttttggttca aatccgagtg
     3361 catggctatt tgcttgattt tgctagtatt gaagggcaaa ggcaaaagca ttatcgtttg
     3421 aaaaatttac ctcagacggt tgaactgaca gtggatgatg tggaggagga tgtggatttg
     3481 accctacctg aaaatcgaag ttatcaagaa gctgattttt ttgaacgcat gtttcgagag
     3541 aactgttaag gccactttta aagatttcca agactatctt tcttcatgag gaaagatagt
     3601 tttttggtat gattttcatt cccaaaatac aaggggaatg tgttacaata gtagtaacag
     3661 ataatagaaa agagaataga tgagaattgc agattatagc gtgaccaagg cagtgctgga
     3721 gcgtcacggt tttaccttta aaaagtcctt cgggcaaaat ttcctgacgg ataccaatat
     3781 ccttcaaaaa attgtggata cggctgaaat tgatgaccag gtcaatgtca tcgaaatcgg
     3841 gccaggtatt ggtgccttga cggagtttct ggctgagcgt gcagcccaag tcatggcttt
     3901 tgagattgac caccgtttgg tgccaatttt ggtagatacc ctgcgtgatt ttgataatgt
     3961 gactgtagtt aacgaagata ttctcaaggt tgatttggcg caacatatcc aaaattttaa
     4021 aaatcctaac ctgccaatca aggtagtggc taatttgcct tactacatca cgacgcctat
     4081 tctcatgcac ttgattgaga gtggcattcc tttttgtgag tttgtggtca tgatgcagaa
     4141 agaagtagcg gaccgcattt cagcccagcc taacaccaag gcttacggta gcttgtctat
     4201 cgccgtgcag tattacatga cagccaaggt tgcctttatc gtgcctcgta cggtctttgt
     4261 gccagcgcca aatgtggatt cagccatctt gaaaatggtg cgtcgtccag agccagccgt
     4321 agcagtagaa gatgagaact ttttctttaa ggtttccaag gctagtttta cccatcgccg
     4381 caagaccttg tggaataact tgacaggtta ctttggtaag actgaagagg tcaaggacaa
     4441 gctgaccaag gctttggacc aggcaggctt gtcaccaagt gtgcgtgggg aagctctcag
     4501 cttggcagaa tttgccggtc tagcagacgc acttaaaggg caaggactct aagatgcagg
     4561 gacaaatcat taaagccttg gcaggtttct actatgtgga gagtgatggc caggtttatc
     4621 aaacacgcgc gcgtgggaat ttccgtaaaa aaggccatac cccttatgtt ggggactggg
     4681 tagatttctc tgccgaggaa aattcagaag gctatatcct caaaattcac gaacggaaaa
     4741 acagtctggt tcgtccgcct attgtcaata tcgatcaagc tgtagtaatc atgtccgtca
     4801 aggaacctga ttttaacagc aatttgctgg atcgtttctt ggttcttttg gagcacaagg
     4861 gcatccatcc cattgtctat atttccaaaa tggatttgtt ggaagatagg ggagaactgg
     4921 atttttacca gcagacctat ggtgacatcg gctatgactt tgtgaccagt aaagaggaac
     4981 tcctgtcttt gttaacaggc aaggttacgg tctttatggg gcagacaggt gttgggaagt
     5041 caactcttct caataaaatc gcaccagacc tcaatcttga aacgggagaa atttcagaca
     5101 gtctaggtcg cggtcgccat accactcgag ctgttagttt ttacaatctc aacgggggta
     5161 aaatcgcaga tacaccagga ttttcatcct tggactatga agtatcaagg gctgaagacc
     5221 tcaatcaggc tttcccagag attgctactg ttagccgaga ttgtaagttc cgtacttgta
     5281 cccataccca tgagccgtct tgtgccgtca aaccagctgt tgaagagggt gttattgcaa
     5341 ccttccgttt tgacaattac ctgcaattcc ttagtgaaat tgaaaatcgt agagaaacct
     5401 ataaaaaagt cagcaaaaaa attccaaaat aaggagaaac ctatgtctca atacaagatt
     5461 gctccgtcaa ttctggcagc agattatgcc aactttgaac gtgaaatcaa acgtctagaa
     5521 gcaactgggg cagaatatgc ccatatcgat atcatggaca gtcattttgt accgcaaatc
     5581 agttttggtg caggtgtggt cgagagcctt cgtcctcata gtaagatggt tttcgattgc
     5641 cacttgatgg tgtcaaaccc tgagcatcat ctggaagatt ttgcgcgtgc aggtgcagac
     5701 atcatcagta tccatgtaga agcaacacct catattcatg gcgccctcca aaaaattcgt
     5761 tcactcggag ttaagccttc agtcgttatc aatcctggca caccagttga agccatcaag
     5821 cacgtccttc atctagttga ccaagtttta gtcatgacgg ttaatccagg ttttggtggg
     5881 caagcctttc tgccagaaac catggataag gttcgtgagt tggttgctct tcgtgaggaa
     5941 aaaggtttga actttgaaat cgaagtggat ggtgggattg atgaccaaac tattgctcaa
     6001 gccaaagaag ctggtgcgac tgtttttgta gcaggttcct atgtctttaa gggagaagtc
     6061 aatgagcgag tacaaactct cagaaaacaa ctggactagg gttgcagttt ttgcaggcgg
     6121 aaaccgcggt cattatcgga cagattttga tgcttttgtt ggggtggatc gaggctcgct
     6181 ctgggtcttg gaagaagact tacctcttgc tctagcagtc ggagattttg attctgtgac
     6241 ggaagaagag cgacaggtga ttcaaaaacg tgcccagtat tttgtccaag cccgaccaga
     6301 aaaggatgat acagatctgg aattggctct cttaaccatc tttgaacaaa atcctcaggc
     6361 tcaggtcact attttcggtg ccttgggtgg ccgtattgac catatgttgg ccaatgtctt
     6421 tctgcctagc aatcctaagt tggcacccta tatgcgtcaa atagaaattg aggatgggca
     6481 aaatttgatt gcctattgtt cagaagggac cagtcagcta gaacctcgtt cagactacga
     6541 ctatctagcc tttatgccag ttcgggatag ccagctgact attcttggag ctaagtatga
     6601 gttgacagag gagaattttt tctttaaaaa agtgtacgct tctaacgaat atatagatag
     6661 ggaagtgtcg gtaacttgcc cagatggtta tgtggtcgta ctgcatagca aggacaggag
     6721 gtaggatgga aagtttactt attctattat taattgccaa cctagctggt ctctttctga
     6781 tttggcaaag gcaggatagg caggagaaac acttaagtaa gagcttggag gatcaggcag
     6841 atcatttgtc agaccagttg gattaccgct ttgaccaagc cagacaagcc agccagttag
     6901 accaaaaaga tttggaagtg gctgtcagcg accgtttgca agaagtgcgg attgaattgc
     6961 accaaggcct gacccaagtc cgtcaagaaa tgacagataa tctcctccaa actagagaca
     7021 agacagacca acgtctccaa gccttgcagg aatcaaatga gcaacgtttg gaacaaatgc
     7081 gccagacggt cgaggaaaaa ctagaaaaga ccttgcagac acgcttacag gcttcctttg
     7141 agacagtttc taaacaactg gagtctgtca atcgtggtct tggagaaatg cagacagttg
     7201 cccgtgatgt cggagctctt aacaaggttc tctctggaac caagacgcga gggattctgg
     7261 gagaattgca actggggcaa attattgaag acatcatgac acctgcccag tacgaacgag
     7321 aatacgcaac ggttgaaaac tctagtgaac gagtggagta tgccatcaag ttacccggac
     7381 aaggcgacca agaatacgtc tatctgccaa ttgactctaa gtttccactg gcagattatt
     7441 accgcttgga agaagcctat gagacaggtg acaaggatga gattgaacgc tgtcgtaagt
     7501 cactcctagc aagcgtcaag cgctttgcca aggatatcaa gagcaagtac atagcaccgc
     7561 ctcggacaac caattttgga atcttgtttg ttccgacaga aggtctttat tcagagattg
     7621 tgcgcaatcc ggtcttcttt gatgatttga gacgggaaga acagattatc gttgcaggac
     7681 caagtaccct atcagccctt cttaactccc tatcagttgg tttcaagacc cttaatatcc
     7741 aaaagagtgc cgaccatatc agcaagactc ttgccagtgt caagaccgag tttggcaagt
     7801 ttggtggtat tctggtcaag gcacaaaaac atctccaaca tgcctctggc aatattgatg
     7861 aattattaaa ccgtcgtacc atagctatcg agcggacgct ccgtcacatt gagttgtcag
     7921 aaggcgagcc tgcgcttgat ctactccatt ttcaagaaaa tgaggaagaa tatgaagatt
     7981 agtcacatga aaaaagatga gctatttgaa ggcttttacc taatcaaatc agctgacctg
     8041 aggcaaactc gagctgggaa aaactaccta gcctttacct tccaagatga tagtggcgag
     8101 attgatggga agctctggga tgcccaacct cataacattg aggcctttac cgcaggtaag
     8161 gttgtccaca tgaaaggacg ccgagaagtt tataacaata cccctcaagt caatcaaatt
     8221 actctccgcc tgcctcaagc tggtgaaccc aatgacccag ctgatttcaa ggtcaagtca
     8281 ccagttgatg tcaaggaaat tcgtgactac atgtcgcaaa tgattttcaa aattgaaaat
     8341 cctgtctggc aacggattgt ccgaaatctc tacaccaagt atgataagga attctactcc
     8401 tatccagctg ccaagaccaa ccaccatgcc tttgaaacgg gcttggccta tcatacggcg
     8461 accatggtgc gtttggcaga cgctattagc gaagtttatc ctcagctcaa taagagcctg
     8521 ctctatgcgg ggattatgtt gcacgacttg gctaaggtca tcgagttgac gggaccagac
     8581 cagaccgagt acacggtcag aggcaatctt cttggacata tcgctctgat tgatagcgaa
     8641 attaccaaga cagttatgga actcggcatc gatgatacca aggaagaagt cgttttgctt
     8701 cgtcatgtca tcctcagtca ccacggcttg cttgaatatg gaagtccagt ccgtccacgt
     8761 attatggagg cagagattat ccatatgatt gacaatctgg atgcaagcat gatgatgatg
     8821 tcaacagctc tggctttggt ggataaagga gagatgtcca ataaaatctt cgctatggat
     8881 aatcgttcct tctataaacc agatttagat taataattta agaaaaatga gcatttttag
     8941 gataagaatg ttcgtttttt tatgtgaata tggtataata agtaaaagac aaaaatgaat
     9001 actcttcgaa aatctcttca aactagggta gtatcgcctt gtcgtatgta tatatgcagg
     9061 tatattacag ggtttgtcag ttctattgac aatctcaaaa cagtgttttg aaccaccagc
     9121 gaccagcttt ctagtttgct ttttgatttt ttgaataaaa atggaatagg aaatagaaat
     9181 gaaattaaga agaagtgatc ggatggttgt catttccaac tatttgatta ataatcctta
     9241 taaactaact agtctcaata cttttgctga aaagtatgag tctgctaaat catccatctc
     9301 agaagatatc gtcattatca aacgcgcctt tgaggaaatt gaaatcggtc atatccagac
     9361 agtgactggg gctggcggag gtgtcatctt cacaccgtct atttcgagtc aggatgctaa
     9421 ggaaatggtt gaagacttgc gtaccaagtt gtcagaaagt gaccgtatct tgccaggtgg
     9481 ttatatctat ctgtctgatt tgcttagcac accagccatc ttgaaaaata ttggtcgtat
     9541 tattgccaaa agctttatgg accaaaaaat tgacgcggtt atgaccgtag caactaaggg
     9601 tgtgccactt gcaaatgcag ttgccaatgt cctcaatgtc tcttttgtca ttgtgcgccg
     9661 tgacctgaaa attaccgaag gttcaactgt tagcgtcaac tatgtttcag gttcaagtgg
     9721 tgaccgtatc gagaaaatgt tcctttcaaa acgtagtctt aaggcaggca gccgtgtctt
     9781 gattgtggat gacttcttga aaggtggcgg aacggtcaat ggtatgatta gtctcttgcg
     9841 cgagttcgac tcagaactgg caggtgtagc ggtctttgcg gacaatgccc aagaagaacg
     9901 tgaaaagcag tttgactaca agtcactctt gaaggtaacc aatattgatg tcaagaacca
     9961 agccatcgat gttgaggttg gcaatatctt tgacgaagat aaataagaga tagaactaaa
    10021 ggttggaacg attgtcccag cctttctttg caaacagaat agaaggaagc ttatgaaaac
    10081 accatttatc aatagagaag agttagaagc gattgttgcc gagttcccga ctccctttca
    10141 cttgtatgat gagaagggga ttcgtgagaa ggcaagagcc gtcaaccaag ctttttcgtg
    10201 gaacaagggc tttaaggaat attttgcagt taaggctact ccaactccag ctattttgaa
    10261 aattctccaa gaagaaggtt gtggtgtgga ctgctctagt tatgtagagc ttttgatgag
    10321 ccataaactg gactttctgg gttctgagat tatgttctct tccaacaaca cgccagacaa
    10381 ggaatacgcc tatgcacgtg aattgggtgc gaccattaac ttggatgcct ttgaagatat
    10441 tgaacatctg gagagagtag caggcattcc agaaatcatc tcttgtcgtt ataatcctgg
    10501 aggcgttttt gaactgggga cagacattat ggacaatcct ggggaggcta agtttggcat
    10561 gaccaaggac cagctctttg aagcctttgc tatcttgaag gaaaaaggag ccaagacttt
    10621 tgggcttcac tccttcctag cgtccaatac cgtgacccat ctctattatc cagagttggc
    10681 tcgtcagctc tttgaactgg ctgttgaaat caaggaaaag ttgggcattt cgctagactt
    10741 tatcaatctt tctggcggta ttggtgttaa ttatcgtcca gaccaggagc cgaacgatat
    10801 cgccttgatt ggtgagggag ttcgtaaggt gtatgaagaa gttcttacgt cagcaggtct
    10861 tggtcaggtc aagattttca ccgaattggg tcgttttatg ctggcacctc acggtgctct
    10921 agtcacaaga gtgactcata agaaggaaac ctaccgtacc tatctaggtg tggatgcctc
    10981 agcagtcaac ctcatgcgtc cagctatgta cggagcttac catcatatta gcaacgtgac
    11041 ccatccagat ggaccagctg aagcggtaga tgtggttggt tcactctgtg aaaacaatga
    11101 taaatttgca gtcaatcgcg aactgcctca tacagaaatc ggtgatttgc tggtcattca
    11161 tgatacaggt gctcacggtt tttcaatggg ttaccagtac aacgccaaac ttcgttctgc
    11221 ggaaatcctc tataccgaag aaggtgaagc tcgccaaatc cgccgtgcag agcgccctga
    11281 ggactatttt gcaaccttat atggattcga ttttgaagaa taatctgata atagcttgaa
    11341 aatgaaattg aaaaacagat tgctttctaa aaaataggca aaaatcttgt ttttccttca
    11401 agtcgtgata taataaaact ataaaacgtt ttcaaggaag gtaacgatat gtctgaagaa
    11461 acaattgatt atggacaagt gacaggaatg gtgcattcga cagaaagctt tgggtcagta
    11521 gatgggcctg gtattcgctt tattgtcttt ttgcagggct gtcacatgcg ttgccagtat
    11581 tgccacaacc cagacacttg ggctatggag tccaataagt cacgtgaacg gacggtagat
    11641 gatgtcttga cagaggcctt gcgctaccgt ggtttctggg gaaataaggg tgggattaca
    11701 gtcagtggag gagaagctct cttgcagatt gatttcctga ttgccctctt caccaaggct
    11761 aaggaacaag gaatccactg taccttggat acctgtgctc ttcctttccg taataaacca
    11821 cgttaccttg agaagtttga caaactcatg gctgtcactg acttggttct tttggatatc
    11881 aaggaaatca acgaagaaca gcacaagatt gtcactagcc aaaccaataa aaatatcttg
    11941 gcttgtgccc agtatctatc agatattgga aaacctgtct ggattcgcca cgtgctagtt
    12001 ccaggattga cagacagaga tgatgacttg attgaacttg gtaagttcgt caagaccctc
    12061 aaaaatgttg ataagtttga aattctacct tatcacacca tgggtgagtt caagtggcgt
    12121 gaacttggaa ttccatattc cctcgaagga gtcaaaccac caacagcaga tcgcgtcaag
    12181 aacgctaaaa aactcatgga taccgaaagt tatcaagatt atatgaaacg tgtacatgga
    12241 tagaaaagaa gcctgatgga aacatcgggc ttttgacttg caaaaagact tagcaaatca
    12301 gctaagcctt tttcttctta tctcgaacgt tgttttccag cgttgcgatt tttgtgtttt
    12361 ttcttgcttg taatagcagt tggttgttca ggggtaacgt cttttcgtcc acttggtgtt
    12421 gagaaagcac ttgcttttgg tgggttcttt gccagttctt cacggacttt tttgcgaagt
    12481 tttggacgaa cgatatagtt gacgataaac tgttggagaa tcatcatgaa accaccgaca
    12541 acccagtaaa gtgtgacact agctggtgag aagagggaga agacgacgat catgagtggg
    12601 ctcatgtaaa tcattttctt gatttgttct ctttgcattt catcttctac cccgtgaagt
    12661 gaaaggagcg attgaagata gtaaaggaca ccagcacagg caaccaaaat catacttgga
    12721 gaacctagag gaatgcctag gtagcttgct tgagcaaccc cttcagtatg ttgggcagca
    12781 aagtagatag cagagaagaa aggcatttga aggaggatag ggaaacatcc tacaccgcca
    12841 aacatgctga taccgtgctc tttttgagca gcaaagagag cttgttgggc ttcgagtttt
    12901 tcttcttgag tagtcgcttc tttgagacgc gtttggtgtg gctcaaggac gtgcttgagg
    12961 gcgttcatct tttcagagtg aagcgttgcc ttccatgatt ggtagatacc aagtggcaag
    13021 ataatcaagc gcacgataat ggttacgatg atgatagcga caccaaagcc tagaccttta
    13081 tcagtagcga agtacttgat agcttcagcc ataggggctc cgatggtatt ccaaataaat
    13141 cctgttggct gacctgtggt tttatcgaca ttgacacaac cagtcaagac aagcaacata
    13201 gccgctccca tagctgagag tgcaaaacgt ttaatagatt tcactgtttt tattccttct
    13261 ttaaaaatta tacctttcta ttctactgtt tttttacaaa atatacaata gttctggaga
    13321 cctaatttgc gattttaaag tcagagtagg gaggaaagtt gcttagtttg acatctaagt
    13381 agcttacttt tgaaaaaggt gtcggtcctt ttcggatttc ttggataaat tttgccatga
    13441 tagcagatga gtctgcttgg gctaagattt ccactgtgcc atcgtcgtta ttccatactc
    13501 gacctgtgat gccaccaatt tcaagtgcca agctgtaaac accccaacga aagccgactc
    13561 cctgtaccct accttgggca atcattctaa ccttttgcat accaaacccc tttgattgtg
    13621 ttataatatt tctatgacta ttataacctc aaaagccaat tctgtggtaa aaaatgccaa
    13681 gaaattacac caaaaaaaat accgcaagtc tgcctatttg attgaaggct ggcacttgtt
    13741 tgaagaagct gtccaagctg gagtgacgat tgagaaaatc tttgccctag aaaattatcg
    13801 aaatcagtta gctgcttttc cgcaaactgt ctggatttca gaggatattt tgctagattt
    13861 ggcggattct cagactccac agggaattgt ggcggtggtt caaaaagaag aagtaggaca
    13921 agctgatttg agtcagggca agttcttgtt tttggaagat gtgcaagatc ctggtaatgt
    13981 aggaactatc attcgaactg cggatgcagc aggttttaca ggagtgattg tttcagataa
    14041 gtcggcagac atctacagtc tcaaaaccct acgttccatg caaggtagtc atttccatct
    14101 gcccatttac cggatgacta gtcaagcgct tcttgacgaa actaaaaagg tagctatccc
    14161 agtgctagca acaaccctat ctaaagattc tgttgattac atagaactgc cttctataga
    14221 aaattttgta ctagttatgg gaaatgaggg tcaaggaatt agtcccctta tggctgaaag
    14281 tgcagaccag ttggtccata ttagcatgaa ggggcaggcc gagagtttga atgttgcggt
    14341 tgcagccggt attttaatct tctatttaag ctaattttaa ctttttttgt tataatcaag
    14401 gaaagatgtt cacagaaaag gagaaatgga tgaatcacac tattatacat gaccgtgcag
    14461 gtctcaatca attttacgct aaggtttatg cctttgttgg tctgggaatc ggactatctg
    14521 ctttggtatc aggccttatg ttgacggttt ttcagtctca gttggtttac tttttgatgc
    14581 aggggcgtct ctggttgacc attgctactt ttgcggagtt ggctttggtt ttcgttgcca
    14641 gtagcatggc ttcaagaaat agtccagctg ctcttccagt atttttactt tactccgttt
    14701 taaacggctt tacccttagt tttgtggtgg ctttctatac tccgggtaca gttttatctg
    14761 cctttgtatc gagcgccctt ctcttctttg tcatggcggc agttggtatc tttaccaaga
    14821 aagacttgag tgggattggt cgagcaatga tggcagctct cattggttta ctgattgcta
    14881 tggtagtcaa tattttcttg gctagtggct tctttgatta tatgattagc gtagccatgg
    14941 ttttggtttt ttcagggttg attgcttggg ataaccaaaa gattcgcctc gcctatgaac
    15001 aatcacaagg tcgtgtagcg acaggctggg tggtgtcaat ggctctcagc atctatcttg
    15061 attttatcaa tcttttccta agtattctac gtatctttgg tcgcaatgac taaaaggtct
    15121 atgaaatcag tgttttgagt gacactgatt ttttttaaat tttctctttt cttttcttgg
    15181 aaataggtgt ataatacgtt tattaaattt ttgaggagtt gtctatgaag aaaagtttta
    15241 tccatcaaca agaagaaatt tcctttgtca aaaacacttt tacccagtat ttgaaagata
    15301 agctagaagt tgtcgaagtt caaggtccta tcttgagtaa ggtcggtgac ggaatgcagg
    15361 acaacctgtc tggtgtggaa aatccagtat cggtcaaggt tctccaaatc cctgatgcta
    15421 cttatgaagt ggtgcactca cttgctaaat ggaaacgcca caccttggct cgttttggct
    15481 ttggtgaagg agagggtctc tttgtccaca tgaaagccct tcgtccagat gaggattcct
    15541 tggatgcaac ccactctgtt tatgttgacc agtgggactg ggagaaggtt atcccaaatg
    15601 gtaagcgtaa catcgtttat ctaaaagaaa cagttgagaa gatttataag gctattcgcc
    15661 tgactgagct agctgttgaa gctcgctatg acatcgagtc tatcttgcca aagcaaatca
    15721 cctttatcca tacagaagaa ttggtagaac gctacccaga cttgacaccg aaagaacgtg
    15781 aaaatgcgat ttgtaaagaa tttggagccg tctttttgat tggtatcggt ggcgagttgc
    15841 cagatggtaa accgcacgat ggacgtgcac cagactatga tgactggaca agcgagtctg
    15901 agaatggcta caagggtcta aatggtgata ttcttgtctg gaatgagtct ttaggtggag
    15961 cctttgagtt gtcttctatg ggaattcgtg tagatgaaga aactcttaga cgtcaggtgg
    16021 aaattacagg tgatgaagac cgcttggaat tggaatggca caagtctttg ttgaatggcc
    16081 tattcccatt gacaatcggt ggaggaattg gacaatctcg tatggccatg ttcctacttc
    16141 gcaagagaca catcggagaa gtgcaaacaa gtgtttggcc tcaagaagtc cgcgatactt
    16201 acgaaaatat tttgtagaga atcgaaccgc aaggttcggt tttctttctc tttttgtcta
    16261 taatttggta taataaacag tatgaaaatc gtatcaggaa tctatgggag acgtcccctc
    16321 aagacactag aaggcaagac aacaagacct acttcggata aggttagggg agccattttt
    16381 aacatgattg gtccctactt tgaaggggga cgagtcttgg acctttatgc aggtagtggt
    16441 ggtttatcta tcgaagcagt atcgcgtggc atgtccagtg ctgttttggt ggagcgagac
    16501 cgtaaggctc agaccatcgt ggctgaaaat atccagatga ccaaggaagt tggaaaattt
    16561 caactcctca agatggatgc agaaagggca ttggaacagg tatctgggga atttgacctc
    16621 gttttcttag accctcccta taccaaggaa caaatcgtag cagatattga aaaaatggct
    16681 gagagagagc ttttttctga agatgttatg gttgtgtgcg agacggataa ggccgttgaa
    16741 cttccagaag aaattgcctg tctgggtatc tggaaggaaa agatttatgg aattagtaag
    16801 gtgacagtct atgtcagata agattggctt attcacaggc tcatttgatc cgatgacaaa
    16861 tgggcatctg gatatcattg aacgggcgag cagactcttt gataagctct atgtcggtat
    16921 tttttttaat ccccacaaac aaggatttct tcctatcgaa aatcgtaaac gggggctaga
    16981 aaaggctttg ggacatctgg aaaatgttga agtcgtggct tctcatgatg aattggtggt
    17041 cgatgttgca aaaagattgg gtgctacttg tctagtgcgt ggtttgagga atgcgtcgga
    17101 tttgcaatat gaagccagtt ttgattacta caatcatcag ctgtcttctg atatagagac
    17161 tatttattta catagtcgac ctgaacatct ctatatcagt tcatcaggcg ttagagagct
    17221 tttgaagttt ggtcaggata ttgcctgcta tgttcccgag agtattttgg aggaaataag
    17281 aaatgaaaaa aaagattaga tggcccttat acgtcattgc ggccttgatt gtgactttct
    17341 tggcatttgt agtgcccttg ccttattata tagaggttcc aggtggttcg gaagatattc
    17401 gccaagtcct taaagtaaat gacacagaag ataaggaagc tggtgcctat caattcgtta
    17461 cggttggtgt tcaacatgcc actttagctc atatgattta tgcttggttg acacctttta
    17521 cagatattcg tagtgctcag gagactacag gtggttcttc cgatgttgaa tttatgcgaa
    17581 tcaatcaatt ctacatgcaa acatcacaaa atatggccaa gtatcaaggt ctaaaaacag
    17641 ctggtaagga tatcgaactc aagtactttg gggtttatgt cttgaatgtg acggataatt
    17701 caacctttaa agggattctc aatatctctg atacggtcac agcagtcaat gatcagacct
    17761 ttgatagttc caaagacttg attgattatg tcagttctca aaaattaggg gactccgtca
    17821 aggtcaccta tgaagaggat gggcaaacca agtctgcaga aggaaagatt atcaccttgg
    17881 aaaatggaaa aaatggaatt ggaatcggct tgattgatcg tacagaggta atcagcaatg
    17941 tcccaattag cttttcaaca gctggtattg gcggcccaag tgctggtctc atgtttagtc
    18001 tagctatcta tactcaaata gctcacccag atcttcgtaa tggtcgtatt gttgccggta
    18061 caggtaccat tgaccgcgat ggtaatgtgg gagacattgg aggtattgat aagaaggttg
    18121 tagcttcggc tagggcaggt gctgctattt tctttgctcc tgataaccct gttagcgaag
    18181 aagaacaaaa ggcgcatccg gacgcgaaaa acaactacca aacagcccta gaagcggcta
    18241 aaacaatcaa gacggatatg aaaatcgtgc ccgttaaaac cctacaagat gcgattgatt
    18301 acttgaaaaa caatccctaa tttttgagtg aagtttccaa actagcgcac aaacagagaa
    18361 gatggtataa tagtcaaatg gttcaattat tattcactct aagtagtcat atgctcttta
    18421 tttatgtgag tttttacctt ttaaaggatc ttgttagatg ggagaaggtt ttaaaagtga
    18481 cagatgagaa tacaagaaaa gttcgtttat tagtagcctt ttttagcatt gtcataggct
    18541 acatcctgag ttctttcttt attagcctgt atcatttgtg gcaagaagcg cttagaggat
    18601 tattatgaaa tcaagagtaa aggaaacgag tatggataaa attgtggttc aaggtggcga
    18661 taatcgtctg gtaggaagcg tgacgatcga gggagcaaaa aatgcagtct tacccttgtt
    18721 ggcagcgact attctagcaa gtgaaggaaa gaccgtcttg cagaatgttc cgattttgtc
    18781 ggatgtcttt attatgaatc aggtagttgg tggtttgaat gccaaggttg actttgatga
    18841 ggaagctcat cttgtcaagg tggatgctac tggcgacatc actgaggaag ccccttacaa
    18901 gtatgtcagc aagatgcgcg cctccatcgt tgtattaggg ccaatccttg cccgtgtggg
    18961 tcatgccaag gtatctatgc caggtggttg tacgattggt agccgtccta ttgatcttca
    19021 tttgaaaggt ctggaagcta tgggggttaa gattagtcag acagctggtt acatcgaagc
    19081 caaggcagaa cgcttgcatg gtgctcatat ctatatggac tttccaagtg ttggtgcaac
    19141 gcagaacttg atgatggcag cgactctggc tgatggggtg acagtgattg agaatgctgc
    19201 gcgtgagcct gagattgttg acttagccat tctccttaat gaaatgggag ccaaggtcaa
    19261 aggtgctggt acagagacta taaccattac tggtgttgag aaacttcatg gtacgactca
    19321 caatgtagtc caagaccgta tcgaagcagg aacctttatg gtagctgctg ccatgactgg
    19381 tggtgatgtc ttgattcgag acgctgtctg ggagcacaac cgtcccttga ttgccaagtt
    19441 acttgaaatg ggtgttgaag taattgaaga agacgaagga attcgtgttc gttctcaact
    19501 agaaaatcta aaagctgttc atgtgaaaac cttgccccac ccaggatttc caacagatat
    19561 gcaggctcaa tttacagcct tgatgacagt tgcaaaaggc gaatcaacca tggtggagac
    19621 agttttcgaa aatcgtttcc aacacctaga agagatgcgc cgcatgggct tgcattctga
    19681 gattatccgt gatacagctc gtattgttgg tggacagcct ttgcagggag cagaagttct
    19741 ttcaactgac cttcgtgcca gtgcagcctt gattttgaca ggtttggtag cacagggaga
    19801 aactgtggtc ggtaaattgg ttcacttgga tagaggttac tacggtttcc atgagaagtt
    19861 ggcgcagcta ggtgctaaga ttcagcggat tgaggcaagc gatgaagatg aataagaaat
    19921 caagctacgt aatcaatcgt ttacttttag tcatcatagt actgatttta ggtactctgg
    19981 ctctaggaat cggtctaatg gtaggttatg gaatcttggg caagggtcaa gatccatggg
    20041 ctatcctgtc tccagcaaaa tggcaggaat tgattcataa atttacagga aattaggctg
    20101 gagaaccagc ctttttctaa agataaggag aaatatgaac aaaaaaacaa gacagacact
    20161 aatcggactg ctagtgttat tgcttttgtc tacagggagc tattatatca agcagatgca
    20221 gtcggcacct aatagtccca aaaccaatct tagtcagaaa aaacaagcgt ctgaagctcc
    20281 tagtcaagca ttggcagaga gtgtcttaac agacgcagtc aagagtcaaa taaaggggag
    20341 tctggagtgg aatggctcag gtgcttttat cgtcaatggt aataaaacaa atctagatgc
    20401 caaggtttca agtaagccct acgctgacaa taaaacaaag acagtgggca aggaaactgt
    20461 tccaaccgta gctaatgccc tcttgtctaa ggccactcgt cagtacaaga atcgtaaaga
    20521 aactgggaat ggttcaactt cttggactcc tccaggttgg catcaggtca agaatctaaa
    20581 gggctcttat acccatgcag tcgatagagg tcatttgtta ggctatgcct taatcggtgg
    20641 tttggatggt tttgatgcct caacaagcaa tcctaaaaac attgctgttc agacagcctg
    20701 ggcaaatcag gcacaagccg agtattcgac tggtcaaaac tactatgaaa gcaaggtgcg
    20761 taaagccttg gaccaaaaca agcgtgtccg ttaccgtgta accctttact acgcttcaaa
    20821 cgaggattta gttccctcag cttcacagat tgaagccaag tcttcggatg gagaattgga
    20881 attcaatgtt ctagttccca atgttcaaaa gggacttcaa ctggattacc gaactggaga
    20941 agtaactgta actcagtaaa agataagcct aaactcctat gtcacttatg gatgtaggag
    21001 ttctttttac tagtttaagc agggctagaa taggtactaa gaaaaaatag taacttctaa
    21061 aactaacttc cagtttccca caacctagct tttagtatga gaaaaacgcg tgaaatcagt
    21121 ggaaatccgt cttagactaa cttccagttt taggagagag atggaagtta cgctaagaaa
    21181 aaaataatat gtattggatt aaattttgtg atataataaa ggataagata ctattttagg
    21241 agaagaacta tggaagaccc aagtagtcag aatttgttgc tacaatttgt tttattgttt
    21301 atcttgacgg tattaaatgc ctttttctca gccacagaaa tggccatggt ttctcttaat
    21361 cgtgcccgag ttgaacaaaa ggcggaagaa ggagacagac gctacatccg cctgctgaag
    21421 gtactagaaa atcctaacca ctttttatca accattcagg taggaatcac cctgattaca
    21481 atcttatcag gggcgagttt aactaatact ctaggacgtg tgattgcctc ttggcttggg
    21541 aatggcgaaa cagcacaagc cgtggcaact tttctatcct tggcattttt gacctatatt
    21601 tccatcgttt ttggggaatt atatcctaag agaatcgctc ttaatctaaa ggatgccttg
    21661 gcaattcgta cagcgccggt tatcattggg atcggaaaac tagtcagtcc ttttgtttgg
    21721 cttttagctg cttcgaccaa tttcttgagc catttgactc ctatgtcatt cgatgatgcg
    21781 gatgaaaaga tgacccgtga tgaaattgcc tatatgctga caaatagtga agaaacattg
    21841 gatgctgatg agattgagat gctacaaggt gtcttttcgc tcgatgaact gatggcacga
    21901 gaggttatgg ttcctcgaac ggatgccttt atggtggata ttcaggatga tagtcaagcc
    21961 attatccaaa gtattttaaa acaaaattat tctcgtatcc cggtttatga tggggataag
    22021 gacaatgtaa ttggaatcat tcacaccaag agtctcctta aggcaggctt tgtggacggt
    22081 tttgacaata ttgtttggaa gagaatttta caagatccac tttttgtacc tgaaactatt
    22141 tttgtggatg acttgctaaa agaactgcga aatacccaaa gacaaatggc aatcttgctg
    22201 gatgaatacg gtggtatggc tggtttagtg actttggaag acctcttaga ggaaattgtt
    22261 ggtgaaattg atgatgaaac ggacaaagca gcaatagatg ttcatcagat tggcgaggat
    22321 acctacattg ttcaaggaac tatgaccctc aatgatttca ataattactt tgatgttgag
    22381 ctggaaagtg atgacgttga taccatcgct ggttattatt tgacaggagt gggaaccatt
    22441 ccaacgactg agaaactcag ctatgaattg gtcagccaaa acaaacagtt tatcttaacc
    22501 aatgataaag tgaagaatgg acgtgttacc aaggtaaaag tccaaatcac agaagtcgaa
    22561 atagaagaag aaacagaata aactagaggc ttttgtcacc aggcgtgacg aagctttttt
    22621 caattgattt caaaagggaa ttatgccatg gaaagaactc tgccacaaac tcgctccaaa
    22681 agtttttaaa gttatacgta tttattcgag ggaaaataaa aaaagccctt caaattgggc
    22741 tttctgttca tttgaaacct gataaatcaa gttatagaag gcggtagacg gatttgaacc
    22801 gacgatcaag cttttgcaga gccgtgcctt accacttggc tataccgcct taacgtttat
    22861 tattatacct tgaaaatgat tttcagtcaa tagttttttc agttttttgt accatagcgt
    22921 gattcgaatg tgatgccaag ttttctatgg gaaaaaattt accaaaaacc tactgaaacc
    22981 ccttgaaaaa tctagctaaa tagggtataa tatgggtaat catttgtcgt aggttttgtc
    23041 tgaaatattg tccagacaag gctcacagca gttaaatctt ctgaaaaagt cagatttagc
    23101 tgctcttttt gtgctttttt tcaagattat gagcatttgt aacagaggct taaagattct
    23161 gaaaattcgt caagaggaca cggtgataag gggtttacaa ccatatgacg attagaaaag
    23221 cctgattgac aaggcttgga acttatttac aaaggagaat catcttggca ggacatgacg
    23281 ttcaatacgg gaaacatcgt acccgtcgta gtttttcaag aatcaaagaa gttcttgact
    23341 taccaaattt gattgaaatt caaactgact cattcaaagc tttcctagac cacggtctta
    23401 aggaagtgtt tgaagatgta ttgccaattt caaacttcac agacacaatg gagttggaat
    23461 ttgttggata tgaaatcaag gaaccaaaat acacgctaga agaagctcgt atccacgatg
    23521 ctagctactc agcaccaatt tttgtaacct tccgtttgat caataaagaa acaggcgaaa
    23581 tcaagaccca agaagttttc tttggtgatt tcccaatcat gacagaaatg ggtactttca
    23641 tcatcaatgg tggtgaacgt attatcgttt ctcagttggt ccgctcacca ggtgtttact
    23701 ttaacgacaa agtagacaaa aatggtaagg tgggctatgg ttcaactgtt atccctaacc
    23761 gtggagcttg gttggaactt gaaagcgact caaaagatat cacctacact cgtatcgacc
    23821 gtactcgtaa gattccattt acaaccttgg ttcgtgctct tggtttctca ggtgatgatg
    23881 aaatctttga tatctttgga gacagcgaat tggttcgcaa cactgttgaa aaagatatcc
    23941 acaagaatcc aatggactct cgtacagacg aagccttgaa agaaatttac gaacgccttc
    24001 gtccaggtga gcctaagacg gctgaaagct cacgtagctt gcttgtagct cgcttctttg
    24061 acccacgtcg ttatgacttg gcagcagttg gtcgttacaa aatcaataaa aaactcaatg
    24121 ttaaaacacg tttgctcaac caaaccattg cagagccatt ggtagaccct gaaactggag
    24181 aaatcttggt agaagctggt acgattatga ctcgtagcgt gattgaaagc attgaaagcc
    24241 atttggatgg cgacttgaac aagattgtct acatcccaaa cgatgcagcc gttgtgactg
    24301 agcctgttgt tcttcaaaaa ttcaaggtta ttgctccaac tgatccagat cgcgtcgtaa
    24361 cgatcattgg taatgctaac ccagatgaca aggttcgtac ggtgactcct gcagatatcc
    24421 ttgctgagat gagctacttc ctcaacttgg ctgaaggact tggccgtgta gatgatatcg
    24481 accaccttgg aaatcgtcgt atccgtgcgg ttggtgaatt gcttgccaac caagtacgtt
    24541 tgggactttc tcgtatggaa cgtaatgtcc gtgaacgtat gtctgttcag gacaatgaag
    24601 tcttgacacc acaacaaatt atcaatatcc gtcctgtaac agctgcagtt aaagaattct
    24661 ttggttcatc acagttgtca cagttcatgg accaacacaa cccgctttct gagttgtctc
    24721 acaaacgccg tttgtcagcc ttaggacctg gtggtttgac tcgtgaccgt gctggatatg
    24781 aagtacgtga cgtgcactac actcactatg gtcgtatgtg tccaatcgag acacctgaag
    24841 gacctaacat cggtttgatc aataacttgt catcttatgg acacttgaac aaatatggtt
    24901 ttgttcaaac accataccgt aaggttgacc gtgaaacagg tgttgtcacg aacgaaattg
    24961 tttggttgac agctgatgaa gaagatgaat atactgtagc tcaggctaac tctcgtctga
    25021 atgaagatgg aacctttgct gagaagattg tcatgggacg tcaccaaggg gtcaaccaag
    25081 agtatccagc taatattgtt gactacatgg acgtttcacc aaaacaggta gttgccgttg
    25141 cgacagcatg tattcctttc ttggaaaacg atgactccaa ccgtgccctc atgggagcca
    25201 atatgcaacg tcaggctgtg ccattgatta atcctcaggc accttacgtt ggtactggta
    25261 tggaatacca agcagcccac gattctggtg cggctgtgat tgctcagtat gatggtaaag
    25321 ttacttacgc agatgctgac aaggtagaag ttcgtcgtga agatggttca ttggatgttt
    25381 accacatcca aaaattccgt cgttcaaact caggtactgc ttacaaccaa cgcactctcg
    25441 taaaagttgg tgatgtcgtt gaaaaaggcg atttcatcgc tgacggacct tctatggaaa
    25501 atggagaaat ggcgcttgga caaaacccaa tcgttgccta catgacttgg gaaggttaca
    25561 acttcgagga tgccgttatc atgagcgaac gcttggtgaa ggacgatgtc tacacatctg
    25621 ttcaccttga agaatacgaa tcagaaacgc gcgatacaaa gcttgggcct gaagaaatca
    25681 ctcgcgaaat tccaaacgtt ggtgaagatg ccctcaaaga ccttgacgaa atggggatta
    25741 tccgtattgg tgctgaggtt aaagaaggtg atattcttgt aggtaaagta acacctaagg
    25801 gtgagaaaga tctttcagct gaagaacgtc tcttgcacgc tatctttgga gacaagtctc
    25861 gtgaagtgcg tgatacttct cttcgtgtac cacacggtgc cgatggtgtc gttcgtgatg
    25921 ttaagatctt tacacgtgta aatggagatg agttgcaatc aggtgttaac atgttggttc
    25981 gtgtttacat cgctcaaaaa cgtaagatta aggtcggaga taaaatggcc ggacgtcacg
    26041 gaaacaaagg ggttgtctct cgtatcgttc ctgtagaaga catgccttac cttccagacg
    26101 gaactccagt cgacatcatg ttgaacccac ttggggtacc atcacgtatg aatatcggtc
    26161 aggttatgga gcttcacctt ggtatggcag ctcgtactct tggtattcac atcgctacac
    26221 cagtctttga tggagcaagt tctgaagatc tttggtcaac tgttaaagaa gcaggtatgg
    26281 atagcgatgc caaaacgatc ctttacgatg gacgtactgg tgaaccgttt gataaccgtg
    26341 tttctgtcgg agtcatgtac atgatcaaac tccaccacat ggttgatgat aaattgcacg
    26401 cgcgttcagt cggaccatac tcaactgtta cccaacaacc actcggaggt aaggctcagt
    26461 ttggtggaca acgtttcggt gagatggagg tttgggctct tgaagcctac ggtgcgtcaa
    26521 atgtccttca agaaatcttg acttacaagt cggacgatat caacggacgt ttgaaagcct
    26581 atgaagctat tacaaaaggc aaaccaattc caaaaccagg tgttccagaa tccttccgag
    26641 ttcttgtcaa agaattgcaa tctcttggtc ttgacatgcg tgtcctagac gaagatgacc
    26701 aagaagtgga acttcgcgac ttggatgaag gaatggacga agatgtcatc cacgtagatg
    26761 accttgaaaa agcccgcgaa aaagcagccc aagaggctaa agcagccttt gaagctgaag
    26821 aagctgagaa agcaacaaaa gcggaagcaa cagaagaagc tgctgaacaa gaataagcag
    26881 ttcacttaga atagaaaggg aagaaatagt ggttgatgta aatcgtttta aaagtatgca
    26941 aatcacccta gcttctccaa gtaaagtccg ttcatggtct tatggagaag tcaaaaaacc
    27001 tgaaacaatc aattatcgta ccttgaaacc agaacgtgaa ggactctttg atgaagtgat
    27061 ctttggtcct acaaaagact gggaatgtgc ttgtggtaag tacaaacgca ttcgttacag
    27121 aggaattgtt tgtgaccgct gtggggttga agtaacgcgt acgaaagttc gtcgtgagcg
    27181 tatgggacat atcgaattga aagctcctgt atctcacatc tggtacttca aggggattcc
    27241 aagccgtatg ggcttgaccc ttgatatgag ccctcgtgcc ctcgaggaag ttatctactt
    27301 tgcggcttat gtggtgattg atcctaagga tacaccactt gagcacaagt ctatcatgac
    27361 agagcgcgaa taccgagagc gcttgcgtga atatggttat ggttcatttg ttgccaagat
    27421 gggtgcggaa gccatccaag accttttgaa gcaagtagat cttgaaaaag aaattgctga
    27481 actcaaagaa gaattgaaaa cagctactgg acaaaagcgt gtcaaagcca tccgtcgttt
    27541 ggatgttttg gatgcctttt acaagtctgg aaacaaacct gaatggatga ttcttaacat
    27601 ccttccggtt atcccaccag atcttcgtcc aatgttgcag ttggatggtg gccgttttgc
    27661 ctcatctgac ttgaatgacc tttaccgccg tgttatcaac cgtaacaacc gtttggctcg
    27721 tttgcttgag ttaaatgcac caggtatcat cgttcaaaat gagaagcgta tgcttcaaga
    27781 agcagttgac gctttgattg acaatggtcg tcgtggtcgt ccaatcacag gaccaggtag
    27841 ccgtccattg aaatcattga gccacatgct taaaggtaaa caaggacgct tccgtcaaaa
    27901 cttgctcggt aaacgtgttg acttctcagg acgttcagtt atcgccgttg gtccaactct
    27961 taagatgtac caatgtggtg tgccgcgtga aatggcgatt gaactcttta aaccatttgt
    28021 catgcgtgaa atcgttgccc gtgatatcgt gcaaaacgtc aaagcagcta aacgcttggt
    28081 ggaacgcgga gatgagcgta tctgggatat ccttgaagaa gtgattaaag aacacccagt
    28141 gcttttgaac cgcgcaccga cccttcaccg tttgggtatc caagccttcg agccagtctt
    28201 gattgatggt aaggctcttc gcttgcaccc acttgtctgt gaagcctaca atgctgactt
    28261 tgacggggac caaatggcca tccacgtacc actttcagaa gaagcacaag cagaagctcg
    28321 tatcctcatg ctagctgctg agcacatctt gaacccgaaa gatgggaaac cggtagttac
    28381 tccatctcag gacatggttt tgggtaacta ctacttgacc atggaagaag ctggtcgcga
    28441 aggtgaagga atggtcttca aagaccgtga cgaagcggtt atggcttacc gcaatggtta
    28501 tgttcacctc cactcacgtg ttggtatcgc aacagacagc ctcaacaagc cttggacaga
    28561 agagcaaaga cataaggtct tgcttacaac agttggtaaa attctcttca acgatatcat
    28621 gccagagggg ctaccatact tgcaagaacc aaacaatgcc aacttgacag aaggtgttcc
    28681 agctaaatac ttcttgccac ttggtggaga tatcaaggaa gctatcagca atcttgagct
    28741 caaccctcca ttcaagaaga aaaaccttgg aaatatcatc gctgaaatct tcaaacgttt
    28801 ccgtacgaca gaaacttctg ccctacttga ccgcatgaag aacctcggtt accaccactc
    28861 aactcttgca ggattgacag tgggtattgc cgatatccca gtcgttgatg acaaggctga
    28921 aatcattgaa gaatcacaca aacgtgtcga acaaatcacc aaacaattcc gtcgtggtat
    28981 gatcacagac gacgagcgtt acaatgctgt tacagctgaa tggcgtgctg cccgtgaaaa
    29041 acttgagaaa cgcttgattg ctaaccaaga tcctaagaac ccaatcgtta tgatgatgga
    29101 ctctggagcc cgtggtaaca tctcaaactt ctcacagctt gccggtatgc gtggtctgat
    29161 ggctgctccg aatggacgta tcatggaatt gccaatcctt tcaaacttcc gcgaaggttt
    29221 gtcggtattg gaaatgttct tctcaactca cggtgctcgt aaaggtatga ccgatacggc
    29281 ccttaagaca gccgactcag gttacttgac tcgtcgtttg gtcgacgttg cccaagacgt
    29341 tatcatccgt gaggacgact gtggaactga ccgtggtctc ttgatccgtt ctatcgcaga
    29401 aggaaaagag atgatcgagt ctctcgaaga gcgtctcaac ggtcgttaca ctaagaaaac
    29461 tgttaaacat ccagaaactg gtgcagtgat tattggtcca aatgaattga ttacagaaga
    29521 caaggcgcgt gaaattgtca atgctggtgt ggaagaagtg actatccgtt ctgtatttac
    29581 atgtaacact cgtcacggtg tctgccgtca ctgttacggt atcaacttgg cgactggtga
    29641 tgcggttgaa gttggtgaag cagttggtac aatcgctgcc caatctatcg gggaacctgg
    29701 tacacagctt acaatgcgta ccttccacac aggtggggtt gcctcaaata ccgatatcac
    29761 tcagggtctt cctcgtgtcc aagaaatctt tgaagcccgc aatcctaaag gggaagcggt
    29821 tattacagag gttaaaggac aagttactgc tatcgaagaa gatgcatcaa ctcgtaccaa
    29881 gaaagtcttt gttaagggtg aaactggcga aggtgaatat gtcgttccat ttacagctcg
    29941 tatgcgtgtc gaagttgggg gccaagtagc gcgtggtgct gctctgacag aaggttctat
    30001 ccaaccaaaa cgtctccttg cagttcgtga tgtcttgtca gttgaaacgt accttctcgg
    30061 tgaagtacaa aaagtttacc gtagccaagg ggtagaaatc ggtgacaaac acatcgaggt
    30121 aatggttcgt caaatgatcc gtaaagtccg tgtcatggat ccaggtgata cagatcttct
    30181 catgggtacc ctcatggata tcaatgactt tacagatgcc aacaaagatg tccttatcgc
    30241 aggtggagtt ccagcgacag gtcgcccagt ccttatggga attaccaaag cctcacttga
    30301 aacaaacagt ttcttgtcag cggcttcctt ccaggaaaca actcgtgtcc ttactgacgc
    30361 agctatccgt ggtaagaaag accatctcct tggacttaaa gaaaatgtta tcatcggtaa
    30421 gatcatccca gctggtactg gtatggcccg ttaccgtaac cttgaaccac atgctgtcaa
    30481 cgaagaagaa taccttaatc ctccagtaga ggaagaagga aatgaagaaa caacagaagt
    30541 agttgtggat actgccgttg aaactgtgga agaaacagta gaataagaga gaatgagaga
    30601 acccgagggt tctctcattc tctcttctga aaactttctc catttttcca cgaaatgtgg
    30661 taaaataaaa gaaagaagct gacaaaggag acgggtatgg aacaaacatt ctttatcatc
    30721 aaaccagatg gtgtaaaaag agggctagtg ggtgaagtgt taaagcgcat cgaacaacgt
    30781 ggatttacaa tcgaaaaatt ggagtttcgt tcacaggttt cagaagagtt gattgaccag
    30841 cactatcagg acttggttgg tcagagtttt tacccaccga ttcgtgaatt catgacttca
    30901 ggtccagttc ttgtgggtgt catttctggt cccaaagtaa tcgaaacttg gcggaccatg
    30961 atgggtgcaa ctcgtccaga agaagcttta ccaggcacta ttcgaggtga ttttgcaaaa
    31021 gctgctggag aaaatgagat tatccaaaat gttgtacatg gttcagattc agaagagtca
    31081 gctaagcgag aaattgctct ttggttttaa gagtggattg gctcaatcaa ttggttaaaa
    31141 gctcatttga atagaaagta tagtcaatta gtttaagaca tgacgcatga tatcaaactt
    31201 tttagttttt gatatggtgc gttttttgat taagtgacta ttagtttgtc tcgctccgtt
    31261 aggtgcgaga caaaaaaaac acccgctatg cgggtgtgcg tcgaaggtta taccaaaaaa
    31321 actccaaacg cgatacaata aaggtgttca atccaattgt aaagcgaaag gagaaaaata
    31381 tggcacaaaa ggttctttta tcctgttctt gtttcaattg actatatttc tatacttttt
    31441 cttcctttct ctgtgaaaat cgcaagtttt tcccaccttt tctcaatact gaaacaacat
    31501 ctttccagaa cttcaataat ttctttttgt gttaaatcat tatttaacta aatctttgga
    31561 ggcaatacgt tttcaaagct acttttttac cataggtttt gagccttcaa cacagattac
    31621 taagccaaaa atgctctaaa tttttgaaat tctaacaaat tccactcaac tttatcatca
    31681 tctttgactt tcacaatatc ttcctcaaaa taacatggaa taaagatagt tttcttttaa
    31741 aacggttgta ggaggaaaat ctctcctaca ctattattga ttctgagaga aaattgcaaa
    31801 atatcatcta ccaaactttc aattattaag attttttgca gtttatctga attagaatcg
    31861 caaggcttct caataatata attgtcgaat tttttcattc taacttcctc tccattattt
    31921 cttcctttat caagaaaaag ttacctcaat attaataaag tacaattcta aattaagtac
    31981 aattctaaat taactccaat tcttgattca tgataatatt tcagtaactc atcgtgcaat
    32041 taaaaacaag gatttttatt tcagatgatc catcgtgaac acttcatcag ccatctccca
    32101 aattgccgga ttatgtgttg cgataatgat tagcctatta tcatctcgaa gagatagcaa
    32161 aatctccata atcaactgag aggttgctgg gtctattgaa gctgttggct catctgccag
    32221 aataaagggt ggattcttta agataatttt tgccaaggca acccgttgcg attctccgcc
    32281 cgataactca aagatgcgct tatctaggtc aagataaacc aggccgacct gttctaaagc
    32341 ctgcttctgc ctcaaccgct gttccgaccg actcaacttt tgaccaatga gacctagctt
    32401 aaggttttct tcaatacttt ggttttcaat taagccaaag ttctggaaga ggtagcccaa
    32461 ttcgtgacgg aaaaaatcac ttgatttata attggccaag tctttacctc ggtaaaaaat
    32521 cgtcccatca taaggttcta atttcccaat catgttcatc aaggttgttt ttccgctacc
    32581 acttgaacca attaaggcat agacttttcc agcctcaaat gtcattgaaa gattcgaaaa
    32641 taactctcgt tctccaaaag atttactcac ctgtttaagt tcaatcatat taacctccct
    32701 tcaaaacaag catggacatc ttgttttctt tctgcatttg gacatgtaac tgtaaaagag
    32761 atagaccagt aaagagtaac aagactaaga aagcaactcc tatctctacc tgaagaaata
    32821 cactcgcaac aaatcccagt aaaaacacac ccagttgagc aaagagataa gtgcgatgga
    32881 tttctaagaa cctgagacct gcaatgcgtt tgataaaaat ggcacgtcta aattcttcaa
    32941 agtagagcct attcatagtg ttaaacaaca agattgaagt tgcaatccca agcacagctc
    33001 ctgctagcat tacccaacgt tccctctgga tattatccaa taatgtgatg tagttgtggt
    33061 aacctgtttg catttctgag acccaatttt caatgccttg tctctggata agctcctggg
    33121 catcagacaa ttgattaaag agaacgtagt tctgtactgc gtctatccaa aacaaaatgg
    33181 actgtggacc agttgattgg ggtgttataa caatgatgat tggatctttc aaaaactgct
    33241 ggtaagaaat aggggtcgta ttatacacaa atcgatcctg acctgattcc aaataaccta
    33301 ccgtagcagt catttgctgt cgttcatctt gactagacat gcgactggtt aagtcgtctt
    33361 caaaaacaga tttataatgt tcttcctctg aacggaggtg ttcaggtagc aataagacaa
    33421 actcccctac attaaggtga ttcatctttt gctcaatagt tgtatctaca ggaatgtttt
    33481 gacgctccaa gtattgcggt gtgacgataa ggacattgcc atttgggttg taatcgtgcc
    33541 attctgtaga ggttatcaag tttttggagg aagccatgcc attttgcaaa gtacggtcaa
    33601 ttaactggtg tctagataag aaagcctttt gttctgaaac agccagatcc atcaattgat
    33661 accaagttct gagtttcctt tgagcttgtt catcaaaacc aggactcgtt ccttcacggc
    33721 tgattgatag ggtaatcagt tgcctttcct gactccaaat ctcttgtcca atccgatgct
    33781 gttgccaggc ttgggaatac tttaagctac tccctattcc cagcgttaca ataataatcg
    33841 ctaatagttg accgattaaa atcaaactaa tgattcctct gacaggcact tgccctttta
    33901 taatctgcat caggtgtatc ttttttatcc caactgcgaa gagttgagcg aaaaacaggg
    33961 agatcgacaa caagatgaga ttataactga gcaagcctgc tcctatggtc attagggatt
    34021 gcgttggaag cgacagaatt ttttgcataa gaatggcgag cacgccagct aaactgaaac
    34081 caacagctat cgctttcaaa tcctcaccaa ctggtctaag gaaaatggac caccgtttct
    34141 ctcctgaaat gaggcgaatg cccgatgacc gtaattggct aatttgacta attaaagtca
    34201 gtgctacaaa ggttaagatg aaaatcaata gactgattaa ctgaaatccg ttactaaaga
    34261 tagccattaa cgtagatagt ttagatggaa ttgtcaggtg catgttagtc aagccaagtt
    34321 gacttagctc ttcccttagc aagtgaatat ctaggtgtcc atcgaataca aaatagtttg
    34381 tttcaacact actactttga gcatcttcta gatttttttc ctgaagccca tcaggcaact
    34441 ttccgtcccc aaaggtcgta taagaaaaaa ctgtagtacc ttctgagtta ggatcttggt
    34501 gttgaatcgc aataaagcta tctgtttctt ctgctagttt ttccaagcgt gtagcaacat
    34561 gctgaaaagt tgtttcaggg gaagaatcac gtacaacaac gttggggtaa taatgggaga
    34621 catatgtatc agtccaaatg gtaaataccc aaacaaagaa caaactagca agcaggttgg
    34681 atatgagtat aaataatttt ttcatagtgc attccttatt ctaaaacaga gggcagaaat
    34741 actcctgccc tctataaact aacaagctta ctgaactatt gataaaagaa tcttcttcaa
    34801 ttattcttta ccatcccgca tcaaatgtaa cttgctcccc aaaatttgta ttaacaaacg
    34861 cataggccgc actacgtgct cctgcataaa ctgttttttg attgttacgt ccattacttc
    34921 caacataggc ccagtgatac tgtgaaccat gataataatt cgaaaatgct ccccaattat
    34981 ttggatcatg atggcctcca taggtccact cgccaccacg gtgagaaact gccaaaacag
    35041 gaagagccaa actaaatgcc aaaacggcaa gggatgatgc aactactttt ctaaatttta
    35101 acatattatt actcctctga atgaaatatt atgaaaatga gcgaaatgca actatatatt
    35161 ttaattagaa atctcagtct gaattatagt atcctatttt ttcctccttt ctaactgcac
    35221 gttcctttct acaggcagag acctaatttt ataaaccaat cttaaatact ttagattttg
    35281 taactcataa ttttcaacag ttggttagct atgaaagcat tttatcaaac atctccattc
    35341 ttatatctat ctggtattgt tacaagctct catcagaata cctttctcta aatagttgaa
    35401 ttttaaagta atacgaaatc aatcagcctt cccaacatca agaccattgg acttcttttg
    35461 cgattgcact cgaatctcca attgtctatt cttctgaata aactgttggt aaggaattgt
    35521 attactaaac ataaaaacac ctagtaatac taatatagct aatttttgaa ttccacgatt
    35581 tttcttcatt cgtgaaaccc ttttctt
//
LOCUS       DAVKGC010000008        35359 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKGC010000008 DAVKGC010000000
VERSION     DAVKGC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.008"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(1..652)
                     /locus_tag="VW049_000320"
                     /pseudo
     CDS             complement(1..652)
                     /locus_tag="VW049_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            647..901
                     /locus_tag="VW049_000321"
     CDS             647..901
                     /locus_tag="VW049_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9431506.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            1095..2429
                     /gene="trmFO"
                     /locus_tag="VW049_000322"
     CDS             1095..2429
                     /gene="trmFO"
                     /locus_tag="VW049_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9431507.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2513..3250
                     /locus_tag="VW049_000323"
     CDS             2513..3250
                     /locus_tag="VW049_000323"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9431508.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            3259..3816
                     /gene="frr"
                     /locus_tag="VW049_000324"
     CDS             3259..3816
                     /gene="frr"
                     /locus_tag="VW049_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9431509.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3876..4730
                     /gene="cvfB"
                     /locus_tag="VW049_000325"
     CDS             3876..4730
                     /gene="cvfB"
                     /locus_tag="VW049_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9431510.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKFRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4739..4954
                     /locus_tag="VW049_000326"
     CDS             4739..4954
                     /locus_tag="VW049_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9431511.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            5040..6008
                     /locus_tag="VW049_000327"
     CDS             5040..6008
                     /locus_tag="VW049_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9431512.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(5986..6165)
                     /locus_tag="VW049_000328"
     CDS             complement(5986..6165)
                     /locus_tag="VW049_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431513.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            6201..6701
                     /locus_tag="VW049_000329"
     CDS             6201..6701
                     /locus_tag="VW049_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9431514.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6704..6964
                     /locus_tag="VW049_000330"
                     /pseudo
     CDS             6704..6964
                     /locus_tag="VW049_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7331..7657)
                     /locus_tag="VW049_000331"
     CDS             complement(7331..7657)
                     /locus_tag="VW049_000331"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431515.1"
                     /translation="MVDVAVDQGGLIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     HTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(7603..8016)
                     /locus_tag="VW049_000332"
     CDS             complement(7603..8016)
                     /locus_tag="VW049_000332"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9431516.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(8017..8442)
                     /locus_tag="VW049_000333"
     CDS             complement(8017..8442)
                     /locus_tag="VW049_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9431517.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWVAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            8619..9042
                     /locus_tag="VW049_000334"
                     /pseudo
     CDS             8619..9042
                     /locus_tag="VW049_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9110..9760
                     /locus_tag="VW049_000335"
     CDS             9110..9760
                     /locus_tag="VW049_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU9431518.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9744..11984
                     /locus_tag="VW049_000336"
     CDS             9744..11984
                     /locus_tag="VW049_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9431519.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSTSYLALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVEGVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPGPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLIAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKENVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            12163..12351
                     /locus_tag="VW049_000337"
     CDS             12163..12351
                     /locus_tag="VW049_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431520.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSWSRYDSYSEYKVG
                     GGWNYGRYEVINYYSGGY"
     gene            12383..12970
                     /locus_tag="VW049_000338"
     CDS             12383..12970
                     /locus_tag="VW049_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431521.1"
                     /translation="MLNITHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNISAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12974..14154
                     /locus_tag="VW049_000339"
                     /pseudo
     CDS             12974..14154
                     /locus_tag="VW049_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     misc_feature    14314..14445
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14461..14991
                     /gene="infC"
                     /locus_tag="VW049_000340"
     CDS             14461..14991
                     /gene="infC"
                     /locus_tag="VW049_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9431522.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            15024..15224
                     /gene="rpmI"
                     /locus_tag="VW049_000341"
     CDS             15024..15224
                     /gene="rpmI"
                     /locus_tag="VW049_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9431523.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15276..15635
                     /gene="rplT"
                     /locus_tag="VW049_000342"
     CDS             15276..15635
                     /gene="rplT"
                     /locus_tag="VW049_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9431524.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15693..16073
                     /locus_tag="VW049_000343"
     CDS             15693..16073
                     /locus_tag="VW049_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9431525.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16323..17123
                     /locus_tag="VW049_000344"
     CDS             16323..17123
                     /locus_tag="VW049_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9431526.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESEMVSQRVCEDGPVFRTGTVVL"
     gene            17134..18072
                     /locus_tag="VW049_000345"
     CDS             17134..18072
                     /locus_tag="VW049_000345"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9431527.1"
                     /translation="MTTNQLQVSLPGLDLKNPIIPASGCFVFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            18114..20147
                     /locus_tag="VW049_000346"
     CDS             18114..20147
                     /locus_tag="VW049_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU9431528.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYADNEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGVFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(20208..21863)
                     /gene="pavA"
                     /locus_tag="VW049_000347"
     CDS             complement(20208..21863)
                     /gene="pavA"
                     /locus_tag="VW049_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9431529.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21968..22465
                     /gene="ybeY"
                     /locus_tag="VW049_000348"
     CDS             21968..22465
                     /gene="ybeY"
                     /locus_tag="VW049_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9431530.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAKKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22446..22841
                     /locus_tag="VW049_000349"
     CDS             22446..22841
                     /locus_tag="VW049_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9431531.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22858..23757
                     /gene="era"
                     /locus_tag="VW049_000350"
     CDS             22858..23757
                     /gene="era"
                     /locus_tag="VW049_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9431532.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23806..24630
                     /gene="mutM"
                     /locus_tag="VW049_000351"
     CDS             23806..24630
                     /gene="mutM"
                     /locus_tag="VW049_000351"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9431533.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24630..25235
                     /locus_tag="VW049_000352"
     CDS             24630..25235
                     /locus_tag="VW049_000352"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9431534.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKSGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            25222..26421
                     /locus_tag="VW049_000353"
     CDS             25222..26421
                     /locus_tag="VW049_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU9431535.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYLVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26421..26570
                     /gene="rpmG"
                     /locus_tag="VW049_000354"
     CDS             26421..26570
                     /gene="rpmG"
                     /locus_tag="VW049_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9431536.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26610..26843
                     /gene="secG"
                     /locus_tag="VW049_000355"
     CDS             26610..26843
                     /gene="secG"
                     /locus_tag="VW049_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9431537.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26945..29299
                     /gene="rnr"
                     /locus_tag="VW049_000356"
     CDS             26945..29299
                     /gene="rnr"
                     /locus_tag="VW049_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9431538.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTIIQTVIKTSFRMTYSDVNDILAVDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            29262..29729
                     /gene="smpB"
                     /locus_tag="VW049_000357"
     CDS             29262..29729
                     /gene="smpB"
                     /locus_tag="VW049_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9431539.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29745..30605
                     /gene="tehB"
                     /locus_tag="VW049_000358"
     CDS             29745..30605
                     /gene="tehB"
                     /locus_tag="VW049_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9431540.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30678..31631
                     /gene="coiA"
                     /locus_tag="VW049_000359"
     CDS             30678..31631
                     /gene="coiA"
                     /locus_tag="VW049_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9431541.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHIANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGKKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIK
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31650..33452
                     /gene="pepF"
                     /locus_tag="VW049_000360"
     CDS             31650..33452
                     /gene="pepF"
                     /locus_tag="VW049_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9431542.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYAYLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33454..34167
                     /locus_tag="VW049_000361"
     CDS             33454..34167
                     /locus_tag="VW049_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9431543.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            34234..35175
                     /gene="prsA"
                     /locus_tag="VW049_000362"
     CDS             34234..35175
                     /gene="prsA"
                     /locus_tag="VW049_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9431544.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
BASE COUNT        10620 a         6569 c         7967 g        10203 t
ORIGIN      
        1 atgattacag gctttatctt caactgttag cggcttgagt ttgctggatt tacgtggagt
       61 ttgtgcttga ggatatatct tcatgagccc ttgataacca ctgtcggcca agattttacc
      121 agcttgtccg atatttctgc aactcatttt gaacaacttc atatcatgac tatagttcac
      181 agcgatatcc aaagaaacaa ttctcccttg acttgtgaca atcgcttgag ccttcatagc
      241 gtgaaatttc tttttaccag aatgattcgc taattctttt tttagggcga ttgatttgta
      301 cttccgtcgc atcaatcatt accgtgtcct cagaactgag aggagttctt gaaatcgtaa
      361 caccactttg aacaagagtt acttcaaccc attggctccg acggataaag ttgctttcgt
      421 gaataccaaa atcagccgca atttgttcat aagttcgata ttctcgcaca tattgaagag
      481 tggccataag aaggtcttct aggcttaatt taggttttcg tccacctttt gcgtgtttaa
      541 gttgataagc tgtttttaat acagctaata tctcttcaaa agtcgtgcgc tgaacaccaa
      601 caagacgctt aaatcgtgca tcagttagtt gtttacttgc ttcataattc atagaactac
      661 tataccatat tttgtttcgc aggaagtcta ttggaaagta agaaatattg aagctgaggc
      721 tattagaaga aattgtgagc gtggtgctat tttttcaggt aaaataaaat atcacgaaga
      781 ttcacagttt aaaggagatc actatgttga atgttatgct gttttagata atacggttat
      841 agcaagagat agaataacag tccctatcga tccgttatgt ggaaaagatt ttatagagta
      901 gcatataatt gattcttaac tggaatactc actatctctt tacatcaaga aaatgactaa
      961 acagggaagt ttgccttctt cccttttttt gttatactag tagaagaaaa aattagaaag
     1021 atttgtgggt gtcaaacagc ccagtggggt gttttaatat ggacttaggt cccacccaaa
     1081 gaggtattag tgtcgtgtct caatcttata tcaatgttat cggtgctggt ttggcaggtt
     1141 ctgaagcagc ttaccaaatc gcagagcgtg gtattccagt taaactatat gaaatgcgtg
     1201 gtgtcaagtc tacaccccag cataaaacag acaattttgc tgagttggtt tgttccaatt
     1261 ctttgcgtgg ggatgctttg acaaatgcag ttggtcttct caaggaagaa atgcgtcgct
     1321 tgggttctgt tatcttggaa tctgctgagg ctacacgtgt tcctgcaggt ggtgcccttg
     1381 cagtggaccg tgatggtttc tctcaaatgg tgaccgaaaa agttgccaac caccccttga
     1441 ttgaagtggt tcgtgatgaa attacagaat tgccgacaga tgttattacg gttatcgcta
     1501 ctggtccttt gacaagtgat gccttggctg aaaagattca tgcccttaat aatggcgatg
     1561 gcttctattt ctacgatgca gcagcgccta ttatcgatgt caacactatc gatatgagta
     1621 aggtctatct caaatctcgt tatgataagg gagaagcagc ctatctcaat gctccaatga
     1681 ccaagcaaga gtttatggat ttccatgaag ccttggtcaa tgcggaagaa gcaccgctca
     1741 attcttttga aaaagaaaag tactttgaag gatgtatgcc aatcgaagtt atggccaaac
     1801 gtggcattaa aactatgctt tatggcccta tgaagccagt cggtcttgag tatccggacg
     1861 actacacagg acctcgtgat ggagaattta aaacacctta tgcggttgtg caacttcgtc
     1921 aggataatgc agctggtagc ctatacaata ttgttggttt ccagacccac ctcaaatggg
     1981 gagaacaaaa gcgtgtcttc caaatgattc cgggtcttga aaatgcggag tttgtccgtt
     2041 atggtgtcat gcatcgcaat tcttacatgg attcaccaaa tcttcttgag cagacttacc
     2101 gttccaagaa acaaccaaat ctcttctttg ctggtcagat gacgggggtg gaaggctatg
     2161 ttgagtcggc ggcttcgggc ttagttgcgg gaattaacgc agctcgtctc ttcaaggaag
     2221 aaagcgaggt tattttcccc gagacgacag cgattggaag cttagctcat tacattaccc
     2281 atgccgacag caaacatttc caaccaatga atgtcaattt tgggatcatc aaggagttgg
     2341 aaggcgagcg tatccgtgat aagaaggctc gttatgaaaa aattgcagag cgtgcccttg
     2401 ccgacttaga ggaatttttg actgtctaat ttttttgaaa gaattgttca tgatactata
     2461 aaaatcttag aaattgtgat aaaataggta ggatgaaaga aggagagtga aaatggcgaa
     2521 tcccaagtat aaacgtattt taatcaagtt atcaggtgaa gcccttgccg gtgaacgtgg
     2581 cgtagggatt gatatccaaa cagttcaaac aatcgcaaaa gagattcaag aagttcatag
     2641 cttaggtatc gaaattgccc ttgttattgg tggaggaaat ctctggcgtg gagaacctgc
     2701 agcagaagca ggtatggacc gtgttcaggc agattacact ggaatgcttg ggactgttat
     2761 gaatgctctt gtgatggcag attcattgca acaagttggg gttgatacgc gtgtacaaac
     2821 agctattgct atgcaacaag tggcagagcc ttatgtccgt ggacgtgccc ttcgtcacct
     2881 tgaaaaaggc cgtatcgtta tctttggtgc tggaattggt tcaccatact tctcgacaga
     2941 tacaacagcg gcccttcgtg cagctgaaat cgaagcagat gccatcctca tggctaaaaa
     3001 tggcgtcgat ggtgtgtaca atgccgatcc taagaaggac aagacagccg ttaagtttga
     3061 agaattgacc caccgtgatg ttatcaacaa aggtcttcgt atcatggact caacagcctc
     3121 aaccctctca atggacaacg acattgactt ggttgtcttc aacatgaacc aatcaggcaa
     3181 catcaaacgt gtcgtatttg gtgaaaatat cggaacaaca gtttcaaata atatcgaaga
     3241 aaaggaataa gaaagaatat ggctaacgta attattgaaa aagctaaaga gagaatgacc
     3301 cagtctcacc aatcacttgc tcgtgaattt ggtggtatcc gtgctggtcg tgccaatgca
     3361 agcttgcttg accgtgtaca tgtagaatac tatggagtcg aaactcctct taaccaaatc
     3421 gcttcaatta cgattccaga agcgcgtgtt ttgttggtaa caccatttga caagtcttca
     3481 ttgaaagaca tcgaacgtgc cttgaacgct tctgatcttg gtatcacacc ggctaatgac
     3541 ggttctgtga ttcgcttggt tatcccagct cttacagaag aaactcgtcg tgaccttgct
     3601 aaagaagtga agaaggtcgg cgaaaatgct aaagtggctg tccgcaatat ccgtcgcgat
     3661 gctatggacg aagctaagaa acaagaaaaa gcaaaagaaa tcactgaaga cgaattgaag
     3721 actcttgaaa aagacattca aaaagtaaca gacgatgctg ttaaacacat cgacgacatg
     3781 actgctaaca aagagaaaga acttttggaa gtctaaaaat aaacagaaaa actcagttgg
     3841 cattgctggc tgagttttat tcgaaagaag gaaatatgaa tacaaatctt gcaagtttta
     3901 tcgttggact gatcatcgat gaaaacgacc gtttttactt tgtgcaaaag gatggtcaaa
     3961 cctatgctct tgctaaggaa gaaggccaac atacagtagg ggatacggtc aaaggttttg
     4021 catacacgga tatgaagcaa aaattccgcc tgacaacctt agaagtgact gccactcagg
     4081 accaatttgg ttggggacgt gtcacagagg ttcgtaagga cttgggtgtc tttgtggata
     4141 caggccttcc tgacaaggaa atcgttgtgt cactcgatat tctccctgag ctcaaggaac
     4201 tctggcctaa gaagggcgac caactctaca tccgtcttga agtagataag aaagaccgta
     4261 tctggggcct cttggcttat caagaagact tccaacgtct tgctcgtcct gcttacaaca
     4321 acatgcagaa ccaaaactgg ccagccattg tttaccgtct caagctgtca ggaacttttg
     4381 tttacctacc agaaaataat atgcttggtt ttattcatcc tagcgagcgt tacgcagagc
     4441 cacgtttggg gcaagtatta gatgcgcgcg ttattggttt ccgtgaagtg gaccgcactc
     4501 tgaacctctc cctcaaacca cgctcctttg aaatgttgga aaacgatgct cagatgattt
     4561 tgacttattt ggaaagcaat ggcggtttca tgaccttaaa tgacaagtca tctccagacg
     4621 acatcaaggc aacctttggc atttctaaag gtcagttcaa gaaagcttta ggtggtctta
     4681 tgaaggctgg taaaatcaag caagaccagt ttgggacaga gttgatttag ggaggcttat
     4741 gagaaaatca ttttacactt ggctcatgac cgagcgcaat cctaaaagta acagtcccaa
     4801 agcaattttg gcagacctcg cttttgaaga ggcagctttt ccaaaacata cagatgattt
     4861 tgatgaggtc agtcgctttt tggaggagca tgccagtttc tcttttaacc taggagattt
     4921 tgacagcatt tggcaggaat atctagaaca ctagcattta ttcattgggt ttgggctagt
     4981 aatttctcca tccctctgct ataataaaaa gaaataaaag gattagagag gttctttatt
     5041 tgaaggaaca ttcaatagac attcaactga gtcatccaga tgacctgttt catctttttg
     5101 gttccaatga acgccatctt cgtttgatgg aagaagagct tgatgttgtg attcatgctc
     5161 gtacggagat tgtccaggtt ttgggagaag agtctgcctg tgaggaagcc cgtcaagtta
     5221 ttcaggcttt gatggtcttg gtaaatcgtg ggatgaccgt tggtacgcca gatgtagtca
     5281 ctgcgattag catggtcaaa aacgatgaaa ttgataagtt tgtcgccctt tacgaagaag
     5341 aaattatcaa ggataatact gggaaaccga tccgcgtcaa aaccttaggt caaaaacttt
     5401 atgtggacag tgtcaaacag catgatgtga cctttggaat tgggcctgca gggacgggga
     5461 agacctttct tgcagttact ttggcagtga ctgcccttaa acgtgggcaa gtcaagcgga
     5521 ttatcttgac acgtccagcg gtggaagcag gagagagtct aggttttctt ccgggtgatc
     5581 ttaaggagaa ggtagatcct taccttcgtc ctgtttacga tgccttgtat cagattctgg
     5641 ggaaagacca aacgactcgt ctcatggagc gtgaaattat cgaaattgcg ccccttgcct
     5701 atatgcgtgg tcggaccttg gatgatgcct ttgtcattct cgatgaggcg caaaacacga
     5761 ccatcatgca gatgaagatg ttcttgacgc gtttaggctt tcattctaag atgattgtca
     5821 atggagatat cagtcagatt gacctgccac gcaatgtcaa gtcaggtttg attgatgccc
     5881 aagagaaact caagaacatc catcagattg actttgttca tttttcagct aaggatgtgg
     5941 ttcgccatcc agttgtcgct cagattatcc gagcttatga atattctact gaagttgcac
     6001 acgactgatt ttgaggaagt tcgcctgcaa aagaatatac ttttgagaga ggaaaatcca
     6061 gttgtatagg ctaaaggttt tatccaaagg tttgagacaa cgattaggca cgatggaaag
     6121 aacttttatg tggctgatga cgatcagtgc atcttcctgt gtcataatca cagggcacaa
     6181 gaaagtagga atttgaaaag atgattgacc aactatctaa gtattacagt tgtaggatac
     6241 taactgaaaa ggatattcca agtattttat ctttatatga aagtaatcct ctgtattttc
     6301 agcattgtcc accagaacca aattttgcaa ctgtaaaaga ggacatgctt tgtctacctg
     6361 aaggtaaagc taaggctgat aagttttttg ttggattttg gaatggatct gaccttgtgg
     6421 ctgttatgga ttttgtctat gcatatcctg atgaggagac tgtttttatt ggtttgttta
     6481 tggttgatca agcctatcag aggaaaggga ttggtagtca tattgtgaca gaagcactag
     6541 cttattttgc taagaacttt cgaaaggcac gtttggctta tgttaaggga aatccgcaat
     6601 ctcagcattt ttgggaaaag cagggcttta aatcaattgg atgcgaggtt aagcaagaac
     6661 tctatacggt tgttatcgct gaacagagcc tagaagatta gaaatggcat caagtaagaa
     6721 ctatttggaa tttgttttgg aacaattatc aggattagat gatgtgactt accgttccat
     6781 gatgggggag tatattcttt acttccgcgg caagattatt ggcggcattt atgacgatcg
     6841 ctttttagtt aaacccgtgc aagcagtctt agataagatt gaccaatctt cttttgagtt
     6901 tccatacaaa ggtgccaaag aaatgatttg agtggaagaa cttgataata agatgtttct
     6961 ataagacctg attttagcta tgtataacca actgccaacg cccaaaccta aaaagaaaaa
     7021 gcaagtgtga acgaagtaaa aaagaagtct gctaaggccc tgtctttgca cgggtaaaat
     7081 tttatatata aaaagaagct gggactaaag agctcagctt cctttggttt atataattgt
     7141 cattacaaga cgaagtggtt gggcgaaact ctgttgactt tattcaattt agagtttctt
     7201 atgcacgatt gagtctggaa cgaaagtctc cagttgcaaa gtatacagta caataaacca
     7261 acgatgtaat agctgatgac acaaagcaca gtgggtagga cttgcgaagt cacccttttc
     7321 ttttcaaaat ttatactaaa tcattgatat cagtgtagtc acgattaagt ccttgagcaa
     7381 ctggtaggct agtcaagtaa ccttgataag tggtcacacc ttgacgcaag ccttcatctt
     7441 cagagattgc ttgtgcgaat cctttgccag ccaaagcttc gatataagga agagtgacat
     7501 tggttagggc gatggttgaa gtgtgggcaa ccgcaccagg gatattggca acggcatagt
     7561 ggagaacacc gtgtttttca tagacgggtt catcgtgcgt tgtcacacgg tcagctgttt
     7621 cgataaggcc accttggtca acagcaacgt caacgataca gagcctggac gcatttgttt
     7681 gaccatctca tctgtcacca attccggtgc ttttgcacca gggatgagaa tggctccaat
     7741 caccacatca gcatctctca cacttgcttc aatgttgaat gaattagata taagagtttg
     7801 aatttgactt ccaaagactt cttctagaac tgagagacgc ttggcactaa tatctaaaat
     7861 agtcacttga gcaccaagac caagggcgat gcgggcagca tgtgtaccga cgacaccatc
     7921 accgatgata gttacttttc cttttggaac acctggtaca ccaccaggta gaacaccaga
     7981 gccaccagct tgcttagtaa ggaagtgagc tccgatttaa acagccatac gacctgcaac
     8041 ctcactcata ggaacgagga gcggtagttg tccttgattg tcacgaacag tttcataggc
     8101 aattcctgtt gtttttgctg ctaacatagc atctgctaat tctggagcag cggccatgtg
     8161 caagtaggtg aagagaagaa gatcgtcgcg caagtaaccg tattcagaac ttaaaggttc
     8221 ttttactttc acaaccaact ctgctaccca agcttcacca gcagtagcga caatctcagc
     8281 tccttgcttt tgatagtcag catcagtaaa gccagaaccg agaccagcat ttgtttcgat
     8341 aaggacacga tgaccacgac taactaagct atgaacacct gcaggtgtga gggcgacacg
     8401 gttttcgtta tttttaattt cttttgggat tccgattaac attgagataa cctacctttc
     8461 aattgacggt cttgttttgg ttgtcacatt ccagttcata aatcaaaaat gtgacggttt
     8521 cattgtatat gaaaccgctt caaaaatcaa gaaaaacttg tcatcccaat ttttttatgc
     8581 tagactagtg aaaatcaagc tctaatggag ggaaaagtat ggaatcaatt tttgtaaaac
     8641 ttgcccagta tccgtctata gaaacggagc gtttattgct tagacctgta actttggatg
     8701 atgcggaagc aatgtttgac tatgcctcgg acaagggtaa tacacgttac acttttccaa
     8761 ccaatcaaag cttggaagaa accaagaata acattgctca gttctacttg gctaatccct
     8821 tgggacgttg gggaatagaa ctaaaaagca atggtcagtt tattggaacc attgacttgc
     8881 acaagtaagg ctaatcccgc gtcaggaaag gtcatggaga aatcaggcat gcgtttttcc
     8941 catgcagaac catatgcttg tatggaccag catgaaaaag gccgaatcgt gacaagagtt
     9001 cattatgtct tgaccaagga agactatttt gcaaataaat aagcagttga aaagaaattt
     9061 ttcgactgtt ttttcttcct cttacgaata atctaagaga ggagaaaata tggaagcaat
     9121 tatcgagaaa atcaaagagt ataaaatcat cgtcatctgt actggtctgg gcttgcttgt
     9181 aggaggattt ttcctgctaa agccagctcc acaaacacct gtcaaagaga cgaatttgca
     9241 ggctgaagtt gcagctgttt ccaaggactc atcgaccgga aaggaagtga acaaggaaga
     9301 gaaggaagaa ccagttgaac aagatctaat cacagtagat gtcaaaggtg ctgtcaaatc
     9361 gccagggatt tatgacttgc ctgtaggtag tcgagtcaat gatgctgttc agaaggctgg
     9421 tggcttgaca gagcaagcag acagcaagtc gctcaatcta gctcagaaag ttagtgatga
     9481 ggctctggtt tacgttccta ctaagggaga agaagcagtt agtcaacaga ctggttcggg
     9541 gacagcttct tcaacaagca aggaaaagaa ggtcaatctc aacaaggcca gtctggaaga
     9601 actcaagcag gtcaagggac tgggaggaaa acgagctcag gacattattg accatcgtga
     9661 ggcaaatggc aagttcaagt cagtagacga gctcaagaag gtctctggca ttggtgccaa
     9721 gactatagaa aagttaaaag actatgttac agtggattaa gaatttctct attcccctaa
     9781 tttacctgag ttttctgtta ctttggcttt actacgccat tttttcaaca tcctatcttg
     9841 ctttgttggg atttgttttt ctgctagtct gtctcttttt ccaatttcct tggaaatctg
     9901 ctagcaaagt tctagtgatt tgtggaatct ttggattttg gtttgttttt caaaattggc
     9961 aacagagtca agcgagtcaa aatctggcgg attctgttga aggggtacgg attttgcctg
    10021 atactattaa ggttaatggt gatagtctat cctttcgtgg caagtctaac ggtcgtgctt
    10081 tccaagtcta ttataaactc cagtccgagg aggagaaaga agcctttcaa gctttaaccg
    10141 acctgcatga gataggacta gaagggaagc tttcggagcc agaagggcag agaaattttg
    10201 gtggctttaa ttaccaagcc tatctgaaga ctcagggaat ttaccagact ctcaatatca
    10261 aaacaatcca gtcacttcaa aagattggca gttgggatat aggagaaaac ttgtccagtt
    10321 tacgtcgaaa ggctgtggtt tggattaaga cgcactttcc aggccctatg cgcaattaca
    10381 tgacaggact cttgctggga catctggaca ccgactttga ggagatgaat gagctttatt
    10441 ccagtctagg aattatccac ctctttgccc tatctggcat gcaggtaggt tttttcatga
    10501 atggatttaa gaaacttctc ttgcgattgg gcttgaccca agaaaagttg aaatggctga
    10561 cttatccctt ttcccttatc tatgcgggac taactggatt ttcagcatcg gttattcgca
    10621 gtctcttgca aaagctactg gctcaacatg gggttaaggg cttggataat tttgccttga
    10681 cggtgcttgt cctctttatt gtcatgccaa actttttctt gatagcagga ggagtcttgt
    10741 cctgcgctta tgcttttatc ctgaccatga ccagcaaaga aggggagggg ctcaaggctg
    10801 ttgctagtga aagtctagtc atctccttgg gcatattgcc cattctatcc ttctattttg
    10861 cggaatttca accttggtct atccttttga cctttgtctt ttcctttcta tttgacttga
    10921 ccttcttacc gctcttgtct attttatttg tcctttcctt tctctatcca gtcattcagc
    10981 tgaactttat ctttgaatgg ttagagggca ttattcgctt ggtctcgcag gtggcaagga
    11041 gaccgcttgt ctttggtcaa cccaacgcat ggcttttaat cttattgtta atttccttgg
    11101 ctttggtcta tgatttgagg aaaaacatta aaggattaac agtattgagt ttattgatta
    11161 caggtctctt tttccttacc aagtatccac tggaaaatga aatcaccatg ctggatgtgg
    11221 ggcaaggaga aagtattttc ctacgggatg taactgggaa aaccattctc atagatgtag
    11281 gtggtaaggc agaatcttat aagaaaatcg aaaaatggca agaaaagatg acgaccagca
    11341 atgcccagcg aaccttgatt ccctatctca aaagtcgagg agtagctaag attgaccagc
    11401 taattttgac taacacggac aaggagaatg ttggagattt gtcagaggtg accaaggctt
    11461 tccatgtagg ggagattcta gtatcaaaag acagtctgaa acagaaggaa tttgtggcag
    11521 aactacaggc gactcaaaca aaggtgcgta gtatgacagt aggggagaac ttgcccattt
    11581 ttggaagtca gttagaagtt ctatctccaa ggaaaatggg agatggagga cacgatgata
    11641 ccctagttct gtatgggaaa ttcttggata agcaatttct cttcacggga aatttggagg
    11701 agaaaggaga gaaggacttg ctgaagcact atccagactt gaaagtaaat gttttgaaag
    11761 ctagccaaca tggcaataaa aaatcatcaa gtccagcctt tctagaaaaa ctcaaaccag
    11821 agcttactct tatctcagtt ggaaagagca atcgaatgaa actcccccat caggaaacat
    11881 tgacacgact ggaaggtatc aatagcaaag tttatcgaac tgaccagcaa ggagctatac
    11941 gttttaaggg gttggatagt tggaaaatcg aaagtgttcg ataggaagga taaatgttgt
    12001 agattagtga aataaactaa aaatttgttg cataataatg ataaaaatgg tataatgaaa
    12061 acgtattcaa tattgaggat gtaaaatcat taaaaattag caaaaatcgt ttaattagtt
    12121 agtttataat ctattggtct tcttcagtcc agtgtatctg ctgtgacagt cactaaaagt
    12181 tacaagtatg attggaatac ggtttgggaa tatagtacca actatcacga ccatcagtat
    12241 gcttggattc cgtcatggtc tcgttatgac agctattctg agtataaagt tggcggaggc
    12301 tggaactacg gtcgttatga ggtcataaac tattacagcg gaggctatta atccttaaag
    12361 agtgagaaaa ggagggctgg atatgttaaa tattactcat gttaccttaa aaacgcgaca
    12421 agtcatcttg caagatgtgg atttcacctt taaaaagggt agggtttatg gtcttcttgc
    12481 tatcaatggc tctggaaaga cgaccctgtt ccgtgccatt agcaatttaa ttcccataag
    12541 tagtggaaat atctcagccc ctccttcttt attttattat gagagtattg aatggctgga
    12601 tggaaactta agtgggatgg actaccttcg tcttatcaaa aacatctgga agtcaggtct
    12661 gaacttgagg gatgaaatcg cctattggga aatgtctgac tatatcagtc ttcccattcg
    12721 caagtattcc ttaggcatga agcaacgctt ggtgattgcc atgtatttcc tcagtcaggc
    12781 caaatgctgg ctcatggatg agattacaaa tggcttagat gagtattatc gacagaagtt
    12841 ttttgatagg ttagcacaaa tcgatagaca agaacagctg gttcttttaa gttcccacta
    12901 taaggaagag ttggttgata tctgcgatag agtagtaacc attcatcagg ggcagataga
    12961 agaggtttag tttatgaaag atgttagtct atttttattg aaaaaagttt tcaaaagccg
    13021 cttaaactgg attatcttag ctttatttgt atctgtactc ggtgttactt tttattttaa
    13081 tagtcagact gcaaactcag tcagcttgga gagagagttg gaaactcgtc ttgtagaccg
    13141 tgagagagtc atcaatgaat atgaagagaa actctcccaa atgtctgata ccagctcgga
    13201 ggaataccag tttgctaaaa ataatttaga cgtgcaaaaa aatcttttga cgcgaaagac
    13261 agaaattcta actttactaa aagaagggcg ctggaaagag gcctactatc tgcagtggca
    13321 agatgaagag aaagattatg aatttgtatc aaatgacccg actgctagct ctgaattaaa
    13381 aatgggggtt gaccgcgaac ggaagattta tcaagccctg tatcccttga acataaaagc
    13441 acatactttg gagtttccga cccacgggat tgatcagatt gtctggattt tagaggttat
    13501 catcccaagc ttgtttgtgg ttgcgattat ttttatgcta acacaactat ttgcagaaag
    13561 atatcaaaat catctggaca cagctcactt atatcctgtt tcaaaagtga catttgcaat
    13621 atcctctctt ggagttggag tgggatatgt aactgtgctg tttatcggaa tctgtggctt
    13681 ttcttttcta gtgggaagtc tgataagtgg ttttggacag ttagattatc cctacccaat
    13741 ttatagctta gtgaatcaag aagtaactat tgggaaaata caagatgtat tatttcctgg
    13801 cttgctctta gctttcttag cctttatcgt cattgtggaa gttgtgtact tgattgctta
    13861 ctttttcaag caaaaaatgc ctgtcctctt tctttcactc attgggattg ttggcttatt
    13921 gtttggcatc caaacaataa gcctcttcaa aggattgcac atctgattcc ctttacttac
    13981 ttgcgttcag tggagatttt atctggaaga ttatctaagc agattgataa tgtcgatcta
    14041 aattggagca tggggatggt cttacttcct tgcctgatta tccttttgtt agtggggatt
    14101 ctatttatcg aaagatgggg aagttcacgt aaaaaggaag tttttaaagt ttagttttcc
    14161 tatagggaag ctaaagaaaa actaacatag ggagagaatc aacttggttc tctctttttg
    14221 attcgaaaac caaaccaaaa tacaaacaca aacttttcaa aaaataactt tttatcttga
    14281 caagagctag aaaacttggt atcatataaa agttgagaaa agcagaagtg agagcttctc
    14341 gccttgtgac attaagttgc ctggccctac ggatgaaaag tttcgaagaa acgctatcat
    14401 aacgtgcggg cttgtatatt tacgagtccg ctattgtttt tctctaataa aacaaaagag
    14461 gtgaaaacca tagcaaagca agacttattc atcaatgatg agattcgtgt acgtgaagtt
    14521 cgcttgattg gtcttgaggg agaacagcta ggcatcaagc cactcagtga agcgcaagct
    14581 ttggctgata acgctaatgt tgacctagta ttgattcaac cccaagccaa accgcctgtt
    14641 gcaaaaatta tggactacgg taagttcaaa tttgagtacc agaagaagca aaaagaacaa
    14701 cgtaaaaaac aaagcgttgt tactgtgaaa gaagttcgtc taagtccaac aattgacaag
    14761 ggtgactttg atacaaaact tcgtaatgca cgtaagttcc ttgaaaaagg aaataaagtt
    14821 aaggtatcta ttcgctttaa gggtcgtatg attacccata aagagattgg tgcaaaagtt
    14881 ttagccgagt ttgctgaagc aactcaagat attgccatca tcgaacaacg tgctaaaatg
    14941 gatggacgtc aaatgttcat gcaattggcg ccagcgactg acaaaaaata attgtcagaa
    15001 agttaaataa aggagaaaaa atcatgccaa aacaaaaaac acaccgcgca tcagctaaac
    15061 gtttcaaacg tacaggttct ggtggactta aacgtttccg tgcttacact tctcaccgtt
    15121 tccacggaaa aactaagaaa caacgtcgtc atcttcgtaa agcatctatg gtgcattcag
    15181 gagattacaa acgtatcaaa gcaatgctta ctcgcttgaa ataatagctt gactgtaagt
    15241 aaacaattct aggaaatatt tggaggaaat aaaacatggc acgtgttaaa ggtggcgttg
    15301 tatcacgcaa acgtcgtaaa cgtattctta aattagcaaa aggttactat ggagctaaac
    15361 acatcttgtt ccgtactgca aaagaacaag taatgaactc ttactactat gcataccgtg
    15421 accgtcgtca gaaaaaacgt gacttccgca aattgtggat tactcgtatc aatgcggcag
    15481 ctcgtatgaa cggactttca tactcacaat tgatgcatgg tttgaaattg gctgagatcg
    15541 aagttaaccg taaaatgctt gctgacttgg ctgttaacga tgcagtagct ttcacagctc
    15601 ttgcagatgc agctaaagca aaacttggta aataacaact gatgagactg gaacaggatt
    15661 gtccagtctt tttaaaaata aaggagaaag atatggcttc aaaaatgcta cacacttgct
    15721 tacgagtaga aaatcttgaa aaatcaatcg cattttatca agatgctttt ggttttaaag
    15781 aattgcgtcg cagagatttt ccagaccatg ccttcacgat tgtctatcta ggtcttgagg
    15841 gtgatgatta tgagttggag ttgacttata actacgatca cggtccgtat gtggttggtg
    15901 atgggtttgc ccatatcgcc ctcagtacac ctgatcttga ggcacttcat caagaacaca
    15961 gtgcaaaagg ctatgaagtg actgagccaa atggtctacc aggaactaca ccaaactatt
    16021 actttgttaa agaccctgat ggatataagg tcgaagtcat tcgtgaaaaa taatttcgtg
    16081 caagttaagt agaatattcg ttttctactt gacttttttt agctgaaaga gtatactaat
    16141 aaaaattcaa cctttaaggg gagtccagag agactcacaa ggtgtcagat aaaagaatag
    16201 tgcaattttc tagaggagac cttttgagtg tgctctcttg tgttgtacga ttttaactga
    16261 ggccttgcac tagcaaggtc ttttctttat ctggtcccct taaaatttaa ggaggaaaag
    16321 ttatgaatct cacatgtaag aagcgtttgg gtgtcattcg gttggaaacc atgaaggtgg
    16381 ttgcacaaga ggaaatcgcg ccagcaatct ttgaattagt cctagaagga gaaatggttg
    16441 aagccatgcg agcaggccaa tttcttcatc tgcgtgtacc agacgatgcc catctcttac
    16501 gtcgtcctat ttcaatttcg tctattgaca aggcaaacaa gcagtgtcac ctcatttatc
    16561 ggattgaggg agctgggact gcaatttttt caaccttaag tcagggagac actcttgatg
    16621 tgatggggcc tcagggaaat ggttttgact tgtctgacct tgatgagcag aatcaggttc
    16681 tccttgttgg tggtgggatt ggtgttccac ccttgcttga ggtggccaag gaattgcatg
    16741 aacgtggagt gaaagtagtg acagtcctcg gttttgctaa taaggatgct gttattttga
    16801 aaacggaatt ggctcagtat ggtcaggtct ttgtaacgac agatgatggt tcttatggca
    16861 tcaagggaaa tgtttccgtt gttatcaatg atttagatag tcagtttgat gctgtttact
    16921 cgtgtggggc tccaggaatg atgaagtata tcaatcaaac ctttgatgat cacccaagag
    16981 cctatttatc tctggaatct cgtatggctt gtgggatggg agcttgctat gcctgtgttc
    17041 taaaagtacc agaaagcgag atggtcagcc aacgcgtctg tgaagatggt cctgttttcc
    17101 gcacaggaac agttgtatta taaggagaaa attatgacta caaatcaatt acaagtgtct
    17161 ctacctggtt tggatttgaa aaatccgatt attccagcat caggctgttt tgtctttgga
    17221 caagagtatg ccaagtacta tgatttagac cttttaggtt ctattatgat caaggcgaca
    17281 acccttgaac cacgttttgg gaatccaact ccaagagtgg cagagacgcc tgctggtatg
    17341 ctcaatgcaa ttggcttgca aaatcctggt ttagaggttg ttttggctga aaagctacct
    17401 tggctggaaa gagaatatcc aaatcttcct attattgcca atgtagctgg tttttcaaaa
    17461 caagagtatg cagctgtttc tcatgggatt tccaaggcaa ctaatgtaaa agctatcgag
    17521 ctcaatattt cttgtcccaa tgttgaccac tgtaatcatg gacttttgat tggtcaagat
    17581 ccagatttgg cttatgatgt ggtgaaagca gctgtggaag cctcagaagt gccagtttat
    17641 gtcaaattaa ccccgagtgt gaccgatatc gttactgtcg caaaagctgc agaagatgcg
    17701 ggagcaagtg gcttgaccat gatcaatact ctggttggaa tgcgctttga cctcaaaacc
    17761 agaaaaccaa tcttggccaa tggaacaggt ggaatgtcag gtccagcagt ctttccagta
    17821 gccctcaaac tcatccgcca agtagcccaa acaacagacc tgcctatcat tggaatgggg
    17881 ggagtggatt cggctgaagc tgccctagaa atgtatctgg ctggggcatc tgctatcgga
    17941 gttggaacag ctaactttac caatccttat gcctgccctg acatcatcga aaatttacca
    18001 aaagtcatgg ataaatacgg tattagcagt ctggaagaac tccgtcagga agtaaaagag
    18061 tctctgaggt aaactgcaat caatctgttc ttgatttttt attagtttgt aatatgaatt
    18121 taggagaatt ttggtacaat aaaataaata agaacagagg aagaaggtta atgaagaaag
    18181 taagatttat ttttttagct ctgctatttt tcttagctag tccagagggt gcaatggcta
    18241 gtgatggtac ttggcaagga aaacagtatc tgaaagaaga tggcagtcaa gcagcgaatg
    18301 agtgggtttt tgatactcat tatcaatctt ggttctatat aaaagcagat gctaactatg
    18361 ctgataatga atggctaaag caaggtgacg actattttta cctcaaatct ggtggctata
    18421 tggccaaatc agaatgggta gaagacaagg gagtctttta ttatcttgac caagatggaa
    18481 agatgaaaag aaatgcttgg gtaggaactt cctatgttgg tgcaacaggt gccaaagtaa
    18541 tagaagactg ggtctatgat tctcaatacg atgcttggtt ttatatcaaa gcagatggac
    18601 agcacgcaga gaaagaatgg ctccaaatta aagggaagga ctattatttc aaatccggtg
    18661 gttatctact gacaagtcag tggattaatc aagcttatgt gaatgctagt ggtgccaaag
    18721 tgcagcaagg ttggcttttt gacaaacaat accaatcttg gttttacatc aaagaaaatg
    18781 gaaactatgc tgataaagaa tggattttcg agaatggtca ctattattat ctaaaatccg
    18841 gtggctacat ggcagccaat gaatggattt gggataagga atcttggttt tatctcaaat
    18901 ttgatgggaa aatagctgaa aaagaatggg tctacgattc tcatagtcaa gcctggtact
    18961 acttcaaatc cggtggttac atgacagcca atgaatggat ttgggataag gaatcttggt
    19021 tttacctcaa atctgatggg aaaatagctg aaaaagaatg ggtctacgat tctcatagtc
    19081 aagcttggta ctacttcaaa tccggtggtt acatgacagc caatgaatgg atttgggata
    19141 aggaatcttg gttttacctc aagtctgatg gcaaaatggc tgaaaaagaa tgggtctacg
    19201 attctcatag tcaagcctgg tactacttca aatctggtgg ctacatggcg aaaaatgaga
    19261 cagtagatgg ttatcagctt ggaagcgatg gtaaatggct tggaggaaaa gctacaaatg
    19321 aaaatgctgc ttactatcaa gtagtgcctg tcacagccaa tgtttatgat tcagatggtg
    19381 aaaagctttc ctatatatcg caaggtagtg tcgtatggct agataaggat agaaaaagtg
    19441 atgacaagcg cttggctatt actatttctg gtttgtcagg ctatatgaaa acagaagatt
    19501 tacaagcgct agatgctagt aaggacttta tcccttatta tgagagtgat ggccaccgtt
    19561 tttatcacta tgtggctcag aatgctagta tcccagtagc ttctcatctt tctgatatgg
    19621 aagtaggcaa gaaatattat tcggcagatg gcctgcattt tgatggtttt aagcttgaga
    19681 atcccttcct tttcaaagat ttaacagagg ctacaaacta cagtgctgaa gaattggata
    19741 aggtatttag tttgctaaac attaacaata gccttttgga gaacaagggc gctactttta
    19801 aggaagccga agaacattac catatcaatg ctctttatct ccttgcccat agtgccctag
    19861 aaagtaactg gggaagaagt aaaattgcca aagataagaa taatttcttt ggcattacag
    19921 cctatgatac gaccccttac ctttctgcta agacatttga tgatgtggat aagggaattt
    19981 taggtgcaac caagtggatt aaggaaaatt atatcgatag gggaagaact ttccttggaa
    20041 acaaggcttc tggtatgaat gtggaatatg cttcagaccc ttattggggc gaaaaaattg
    20101 ctagtgtgat gatgaaaatc aatgagaagc taggtggcaa agattagtac tataagtgaa
    20161 tatgatttga gtgaatagta agttaaaaat cctgatttca agtaaaatca ggattttttc
    20221 atggatgcaa tttttttgga gtctggtgtg acgcggaggg tcttttgtcc tgtgtaagtg
    20281 acaaagccgg gttttccacc agttggttta ttgagttttt tgacttcaat catatctacc
    20341 tgcaccagat tcgacaggcg cccttgagag aagtaggcag ctaactctgc tgcgtctgtc
    20401 ttgactgcat cagatgggtc aagatttcct gagatgacaa catggcttcc aggaatgtcc
    20461 ttagcatgga accaaagttc ctccttgcgg gccattttaa aggtcaattc ctcattttga
    20521 agattgtttc gtccgacata gatgatggtt ttgccatcgc ttgctagata ttgttctagt
    20581 tttttgcgtt tctggatttt ctcccgttgt cttctgcgga taaaacctgt ttgaatcaat
    20641 tcttcacgga tttcagcgat ttcttccagt ccagcttggt tgaggacggt ttctacactt
    20701 tccagataga gaatagtggc tttggtttct tcaatcaaat cagtcaagta tttgacagct
    20761 tctttgagtt tctgataccg tttaaaatag cgttgggcat tctggttggg agtcagagcc
    20821 ttatcaagcg caatcatgat aggttggttg gtatagtagt tgtctaggat aacctggtct
    20881 tggtcgttag gcacttggtg gaggaaggtt gtcagcaatt ctcctttttg acgaaattct
    20941 tcagcgttgt ctgtcgccag taactctttt tcctgttttt tgagtttgtg tcggtttttc
    21001 tgaagttcat tttcaacacg acgaatcagt tcactggcct gctgtttgac gcggtcgcgc
    21061 tcagccttat ccttatagta ggtgtccaac aaatcagaaa gatttgcaaa aggctctccc
    21121 acctgatttg caaaaggaac tggactgaag gaagtctcag tcaagcatgg cttggtttct
    21181 tgattgaaaa aatttcggaa agcggaaagt ttttcactaa ccagtatcct ttccaattca
    21241 tttgccgtat cgcgtcccag accttgaaag aggctttgaa gattttttgc tgttagttct
    21301 tgggtttgta ggatttcaaa gagcttttca tccttgatag taaaaggatt gagagatttt
    21361 gtacttggcg gagcgatata ggtcgatcct ggaagtaagg tgcggtagct attttgtgaa
    21421 aaaccgacgt gtttgataac ttcgaggatt ttatgactgc ttttatcgac cagtagaata
    21481 ttactgtgtt ttcccataat ttcaataatc aaggtagcct ggatatggtc tccaatctcg
    21541 tttttattgg aaactgtaat ttccacaata cggtcatttt ccacttgctc aatcgactca
    21601 atcagggccc cctgcaaata ctttctcaaa accatgataa aggtagaagg ttgagctgga
    21661 ttttcaaaag tcgtttgggt cagctgaatg cgtccaaaaa ctggatgggc agaaaggagc
    21721 aggcgatggc tttggcgatt gctgcggatt tgcaagacca actcttgttc aaaaggctga
    21781 ttgattttct ggatgcgacc attcactaat tcgcttcgca attcctcaac tatgtggtgt
    21841 aaaaaaaatc cgtcaaatga catcgttctc tccttgtgat tgtattccat agtattatat
    21901 caaaaaggta gaataaaatc atggaaatgt ggtataataa agccaagtaa agagaaacga
    21961 gaagcacatg tatattgaaa tggtagatga aactggtcaa gtttcaaaag aaatgttgca
    22021 acaaacccaa gaaattttgg aatttgcagc caaaaaatta ggaaaagaag acaaggagat
    22081 ggcagtcact tttgtgacca atgagcgtag tcatgaactt aatctggagt accgtgacac
    22141 cgaccgtccg acagatgtca tcagccttga gtataaacca gaattggaaa ttgcctttga
    22201 cgaagaggat ttgcttgaaa atccagaatt ggcagagatg atgtctgagt ttgatgccta
    22261 tattggggaa ttgttcatct ctatcgataa ggctcatgag caggccgaag aatatggtca
    22321 cagctttgag cgtgagatgg gcttcttggc agtacacggc tttttacata ttaacggcta
    22381 tgatcactat actccggaag aagaagcgga gatgttcggt ttacaagaag aaattttgac
    22441 agcctatgga ctcacaagac aataaacgaa aatggaaaaa tcgtgacttg atatccagtt
    22501 tagaatttgc catcacaggg atttttactg ctatcaagga agaacgaaat atgcgaaaac
    22561 atgcagtgac ggctctagtg gtcatccttg caggttttgt ttttcaggtg tcacgaatcg
    22621 aatggctctt tctcctattg agtattttct tggtagtagc ctttgagatt atcaactctg
    22681 ctattgaaaa tgtggtggat ttggccagtc actatcactt ttccatgctg gctaaaaatg
    22741 ccaaggatat ggcggccggc gcggtattag tggtttctct tttcgcagcc ttaacaggcg
    22801 cattgatttt tctcccacga atctgggatt tattatttta aacagtaaga ggaaattatg
    22861 acttttaaat caggctttgt agccatttta ggacgtccca atgttgggaa gtcaaccttt
    22921 ttaaatcacg ttatggggca aaagattgcc atcatgagtg acaaggcgca gacaacgcgc
    22981 aataaaatca tgggaattta cacgactgat aaggagcaaa ttgtctttat cgacacacca
    23041 gggattcaca aacctaaaac agctctcgga gatttcatgg ttgagtctgc ctacagtacc
    23101 cttcgcgaag tggacaccgt tcttttcatg gtgcctgctg atgaagcgcg tggtaagggg
    23161 gacgatatga ttatcgagcg tctcaaggct gccaaggttc ctgtgatttt ggtggtgaat
    23221 aaaatcgata aggtccatcc agaccagctc ttgtctcaga ttgatgactt ccgtaatcaa
    23281 atggacttta aggaaattgt tccaatctca gcccttcagg gaaataacgt gtctcgtcta
    23341 gtggatattt tgagtgaaaa tctggatgaa ggtttccaat atttcccgtc tgatcaaatc
    23401 acagaccatc cagaacgttt cttggtttca gaaatggttc gcgagaaagt cttgcatcta
    23461 actcgtgaag agattccgca ttctgtagca gtagttgttg actctatgaa acgagacgaa
    23521 gagacagaca aggttcacat ccgtgcaacc atcatggtcg agcgcgatag ccaaaaaggg
    23581 attatcatcg gtaaaggtgg cgctatgctt aagaaaatcg gtagcatggc ccgtcgtgat
    23641 atcgaactca tgctaggaga caaggtcttc ctagaaacct gggtcaaggt caagaaaaac
    23701 tggcgcgata aaaagctaga tttggctgac tttggctata atgaaagaga atactaagta
    23761 gaggtaggct catgcctgct tcttgttttt acagaaggag gacttatgcc tgaattacct
    23821 gaggttgaaa ccgtttgtcg tggcttagaa aaattgatta taggaaagaa gatttcgagt
    23881 atagaaattc gctaccccaa gatgattaag acggatttgg aagagtttca aagggaattg
    23941 cctagtcaga ttatcgagtc aatgggacgt cgtggaaaat atttgctttt ttatctgaca
    24001 gacaaggtct tgatttccca tttgcggatg gagggcaagt atttttacta tccagaccaa
    24061 ggacctgaac gcaagcatgc ccatgttttc tttcattttg aagatggtgg cacgcttgtt
    24121 tatgaggatg ttcgcaagtt tggaaccatg gaactcttgg tgcctgacct tttagacgcc
    24181 tactttattt ctaaaaaatt aggtcctgaa ccaagcgaac aagactttga tttacaggtc
    24241 tttcaagctg cccttgccaa gtccaaaaag cctatcaaat cccatctcct agaccagacc
    24301 ttggtagctg gacttggcaa tatctatgtg gatgaggttc tctggcgagc tcaggttcat
    24361 ccagctagac cttcccagac tttgacagca gaagaagcga ctgccattca tgaccagacc
    24421 attgctgttt tgggccaggc tgttgaaaaa ggtggctcca ccattcggac ttataccaat
    24481 gcctttgggg aagatggaag catgcaggac tttcatcagg tctatgataa gactggtcaa
    24541 gaatgtgtac gctgtggtac catcattgag aaaattcaac taggcggacg aggcacccac
    24601 ttttgtccaa actgtcaaag gagggactga tgagaaaaat catcggaatc actgggggaa
    24661 ttgcctctgg taagtcaact gtgacaaatt ttctaagaca gcaaggcttt caagtagtgg
    24721 atgccgacgc agtcgtccac caactacaga aatctggtgg tcgtctgttt gaggctctag
    24781 tacagcactt tgggcaagaa atcattcttg aaaacggaga actcaatcgc cctctcctag
    24841 ctagtctcat cttttcaaat cctgaagagc aaaaatggtc taagcaaatt caaggggaga
    24901 ttatccgtga ggaactggct actttgagag aacagttggc tcagacagaa gagattttct
    24961 tcatggatat tcccctactt tttgagcagg actacagcga ttggtttgct gagacttggt
    25021 tggtctatgt ggaccgagat gcccaagtag aacgcttaat gaaaagggac cagttgtcca
    25081 aagatgaagc tgagtctcgt ctggcagccc agtggccttt agaaaaaaag aaagatttgg
    25141 ccagccaggt tcttgataat aatggcaatc agaaccagct tcttaatcaa gtgcatatcc
    25201 ttcttgaggg aggtaggcaa gatgacagag attaactgga aggataatct gcgtattgcc
    25261 tggtttggta attttctgac aggagccagt atttctttgg ttgtaccttt tatgcccatc
    25321 ttcgtggaaa atctaggtgt agggagtcag caagtcgctt tttatgcagg cttagcaatt
    25381 tctgtctctg ctatttccgc ggcgctcttt tctcctattt ggggtattct tgctgacaaa
    25441 tacggccgaa aacccatgat gattcgggca ggtcttgcta tgactatcac tatgggaggc
    25501 ttggcctttg tcccaaatat ctattggtta atctttcttc gtttactaaa cggtgtattt
    25561 gcaggttttg ttcctaatgc aacggcactg atagccagtc aggttccaaa ggagaaatca
    25621 ggctctgcct taggtacttt gtctacaggc gtagttgcag gtactctaac tggtcccttt
    25681 attggtggct ttatcgcaga attatttggc attcgtacag ttttcttact ggttggtagt
    25741 tttctatttt tagctgctat tttgactatt tgctttatca aggaagattt tcaaccagta
    25801 gccaaggaaa aggctattcc aacaaaggaa ttatttacct cggttaaata tccctatctt
    25861 ttgctcaatc tctttttaac cagttttgtc atacaatttt cagctcaatc gatcggccct
    25921 attttggctc tttatgtacg cgacttaggg cagacagaga atcttctttt tgtctctggt
    25981 ttgattgtgt ccagtatggg cttttccagc atgatgagtg caggagtcat gggcaagcta
    26041 ggtgacaagg tgggcaatca tcgtctcttg gttgtcgccc agttttattt agtcatcatc
    26101 tatctcctct gtgccaatgc ctctagcccg cttcaactag gactttatcg attcctcttt
    26161 ggattgggaa ccggtgcctt gattcccggg gttaatgccc tactcagcaa aatgactccc
    26221 aaagccggca tttcgagggt ctttgccttc aatcaggtat tcttttatct gggaggtgtt
    26281 gttggtccca tggcaggttc tgcagtagca ggtcaatttg gctaccatgc tgtcttttat
    26341 gcgacgagcc tttgtgttgc ctttagttgt ctctttaacc tgattcaatt tcgaacatta
    26401 ttaaaagtaa aggaaatcta gtgcgagtaa aaattaatct caaatgctcc tcttgtgaca
    26461 gtatcaatta cctaaccagt aaaaattcaa aaacccatcc agacaagatt gaggttttaa
    26521 agtattgtcc caaggaaaga aaagtaaccc tacatcttga atctaagtag attttatggt
    26581 aaaataatag ggattttaag gagtttgata tgtataacct attattaacc attttattag
    26641 tattatctgt tgtgattgtg attgcaattt tcatgcaacc aaccaaaaac caatccagca
    26701 atgtatttga tgccagttca ggtgatttgt ttgaacgcag taaagctcgc ggttttgaag
    26761 ctgtaatgca gcgtttgaca gggattttag tctttttctg gctagccatt gccttagcat
    26821 tgacggtatt atcaagtaga taagaaaata atgggcagga ctaggtcttt gcctcttttt
    26881 atttttaaag gatgtttgag aaggttttac agtaaaagaa aattaaaaaa tctagaaaga
    26941 aaatatgaaa gatagaataa aagaatattt acaagacaag ggaaaggtga ctgttaatga
    27001 tttggctcag gctttgggaa aagacagttc caaggatttt cgtgagttga ttaaaacctt
    27061 gtccttaatg gaaagaaagc accagattcg ctttgaagaa gatggcagtc tgaccttgga
    27121 agccaagaaa aaacatgaga ttaccctcaa ggggattttt catgcccata aaaatggttt
    27181 tggttttgtc agcctagaag gggaagagga cgaccttttt gtagggaaaa atgatgtcaa
    27241 ctatgctatt gatggtgata ccgtcgaggt agtgattaag aaagtcgctg accgcaataa
    27301 gggaacagca gcagaagcca aaattattga tatcctagaa cacagtttga caacagttgt
    27361 cgggcaaatc gttctggatc aggaaaagcc taagtatgct ggctatattc gttcaaaaaa
    27421 tcagaaaatc agtcaaccga tttatgttaa gaaaccagcc ctaaaattag aaggaacaga
    27481 agttctcaaa gtctttatcg ataaataccc aagcaagaaa catgatttct ttgtcgctag
    27541 tgtactcgat gtagtgggac actcaacgga tgtcggaatt gatgttcttg aggtcttgga
    27601 atcaatggac attgtatccg agtttccaga agctgttgtt aaggaagcag aaagtgtgcc
    27661 tgatgctccg tctcaaaagg atatggaagg tcgtctggat ctaagagatg aaattacctt
    27721 taccattgac ggtgcggatg ccaaggactt ggacgatgca gtgcatatca aggctctgaa
    27781 aaatggcaat ctggagcttg gggttcacat tgcagatgtt tcttattatg tgaccgaggg
    27841 gtctgccctt gacaaggaag cccttaaccg tgcgacttct gtttacgtga cagaccgagt
    27901 ggtgccaatg cttccagaac gactatcaaa tggcatctgc tctctcaatc cccaagttga
    27961 ccgcctgacc cagtctgcta ttatggagat tgataaacat ggtcgtgtgg tcaactatac
    28021 cattatacaa acagttatca agaccagttt tcgtatgacc tatagcgatg tcaatgatat
    28081 cctagctgtc gatgaagaaa agagaaaaga atatcataaa attgtatcaa gtatcgaact
    28141 catggccaag cttcatgaaa ctttagaaaa catgcgtgtg aaacgtggag ctctcaattt
    28201 tgataccaat gaagcgaaga ttttagtgga taaacaaggt aagcctgttg atatcgttct
    28261 tcggcagcgt ggcattgccg agcggatgat tgagtctttt atgttgatgg ctaatgaaac
    28321 agttgccgaa catttcagca agttggattt gccttttatc tatcgaattc acgaggagcc
    28381 taaggctgaa aaggttcaga agtttattga ttatgcttcg agttttggct tgcgtattta
    28441 tgggactgcc agtgagatta gtcaggaggc acttcaagac atcatgcgtg ctgttgaggg
    28501 agaaccttat gcagatgtat tgtccatgat gcttcttcgc tctatgcagc aggctcgtta
    28561 ttcggagcac aatcacggcc actatggact agctgctgac tattatactc actttaccag
    28621 tccaattcgt cgttatccag accttcttgt tcaccgtatg attcgggatt acggccgttc
    28681 taaggaaata gcagagcatt ttgaacaagt gattccagag attgcgaccc agtcttccaa
    28741 ccgtgaacgt cgtgccatag aagctgagcg tgaagtcgaa gccatgaaaa aggctgagta
    28801 tatggaagaa tacgtgggtg aagagtatga tgcagttgta tcaagtattg tcaaattcgg
    28861 tctctttgtc gaattgccaa acacagttga aggcttgatt cacatcacta atctacctga
    28921 attttatcat ttcaatgagc gtgatttgac tcttcgtgga gaaaaatcag gtatcacttt
    28981 ccgagtgggt cagcagatcc gtatccgtgt tgaaagagcg gataaaatga ctggagagat
    29041 tgatttttca ttcgtaccta gtgagtttga tgtgattgaa aaaggcttga aacagtctag
    29101 tcatagtggc agagggcgtg gttcaaatcg tcgttcggat aagaaggaag acaagagaaa
    29161 atcaggacgc tcaaatgata agcgtaagca ttcacaaaaa gacaagaaga aaaaaggaaa
    29221 gaaacctttt tacaaggaag tagctaagaa aggagccaag catggcaaag ggcgagggaa
    29281 aggtcgtcgc acaaaataaa aaggcacgcc acgactatac aatcgtagat acgctagagg
    29341 cagggatggt cctgactgga actgaaatca agagtgtacg agctgctcga attaatctca
    29401 aggatggctt tgctcaagtg aaaaatggag aagtttggct gagcaatgtt catatcgcgc
    29461 cttacgaaga gggcaatatc tggaaccagg aaccagaacg tcgtcgtaaa ctcctgctcc
    29521 ataaaaagca aattcaaaaa ttggaacaag agaccaaagg gacaggaatg accttagttc
    29581 cccttaaggt ctatataaaa gatggctacg ctaagcttct tttaggactt gccaaaggga
    29641 agcatgacta tgacaaacgg gagtctatca aacgtcgtga gcaaaatcga gatatcgcgc
    29701 gtgtgatgaa agctgttaat cagcgataaa aagaggaatt gaaaatggaa aaattagttg
    29761 cctataaacg catgcctttg tggaataaac aaacaatgcc tgaagctgtt cagcaaaagc
    29821 acaatacaaa agttgggact tgggggaaaa ttactgtctt gaagggagct ctcaagttta
    29881 ttgaattgac agaagaaggg gaagttctag ctgaacacct ctttgaagca ggggcagaca
    29941 atccaatggc ccaacctcaa gcctggcacc gagtggaagc tgccacagat gatgtggaat
    30001 ggtacttgga attttattgt aaacctgagg attattttgc taaaaaatac aataccaatc
    30061 ctgttcattc agaggtccta gaggccatgc agacagtgaa acaagggaaa gctttggatt
    30121 tgggttgtgg tcagggacgt aattctcttt ttctagccca gcaagatttt gatgtgacgg
    30181 ctgtagatca aaatggacta gctcttgaaa tcttgcaaag cattgtggag caggaagatt
    30241 tggacatgcc tgttggcctt tacgatatca attcagctag cattgaacaa gaatatgatt
    30301 ttatcgtttc aacagttgtt ctcatgtttc tacaagcgga ccgcattcca gctattattc
    30361 aaaatatgca ggagaaaacc agtgttggtg gttacaacct tatcgtttgt gccatggaca
    30421 cggaggatta tccttgctcg gttaacttcc cattcacctt taaagaagga gaactggcag
    30481 actattacaa ggattgggaa ttggttaagt acaatgaaaa tccaggccat ttgcatcgtc
    30541 gcgatgagaa tggcaatcgt attcaactac gctttgcgac cttactagct aagaaaatca
    30601 agtaaacaca catgaagatt aggaattttc ctgatctttt ttctttttta cgaatgatat
    30661 agaaaaggag ggaattcatg tttgttgcga gagatgctag gggagaattg gtaaatgtgt
    30721 tagaggataa acttgagaag caagcataca cctgcccagc ttgtggaggc cagatccatt
    30781 tgcgtcaagg accaagtgta cggacgcatt ttgcccataa atccttaaaa gactgtgatt
    30841 ttttctttga aaatgaaagt ccagaacaca tagccaataa ggaatccctc tatcactggt
    30901 tgaaaaaaga gacaaaggtt caattagagt acccgctttc agaacttaaa cagattgcgg
    30961 atgtatttgt aaatggcaat ctagctctag aagttcagtg tagtcccttg cctcagaaag
    31021 tccttaaaga gcgaagtgag ggctatcgta gtcagggtta ccaagtactg tggttgctgg
    31081 gtaaaaaact gtggctcaag gagcgtttga ctcgtctaca gcaaggtttt ctttatttca
    31141 gtcaaaacat gggcttttat gtttgggaat tagacaagga aaaacaagtt ttaagactca
    31201 aatacctgat ttaccaggat ctccgcggta aactccatta tcaaatcaag gaattttcct
    31261 atggtcaagg tagtttattg gaaatattgc gtcttcccta taagagacaa aaaatatctc
    31321 attttacagt ttctgaggac aaggacatct gtcgctatat ccggcaacaa ctttattatc
    31381 aaaatctctt ttggatgaaa gaacaagcag aagcctatca aaagggagaa aatatcctga
    31441 cttatggact gaaagaatgg tatccacaaa ttcgaccaat agtgggcaaa tttttccaga
    31501 ttaaacaaga cttgactagc tattatcagc acttttatac ctattaccaa aaaaatcctc
    31561 aaaatgattg gcaaaagctt tatccaccag ccttttatca gcaatatttc ttgaaaaata
    31621 tggtagaata gaaaggatgg aggaatctaa tggtattaca aagaaatgaa ataaatgaaa
    31681 aagatacatg ggatctatca acgatctacc caactgacca ggcttgggaa gaagccttaa
    31741 aagatttaac agaacaattg gagacagtag cccagtatga aggccatctc ttggatagtg
    31801 cggataacct actagaaatc actgaatttt ctcttgaaat ggaacgccag atggagaagc
    31861 tttacgttta tgctcatatg aagaatgacc aggatacacg tgaagctaag tatcaagagt
    31921 actatgccaa ggccatgaca ctctacagcc agttagacca agccttttca ttctatgagc
    31981 ctgaatttat ggagattagc gaaaagcagt atgctgactt tttagaagct caaccaaagc
    32041 tgcaggttta tcaacactat tttgacaagc tcttgcaagg caaggatcac gttctctcac
    32101 aacgtgaaga agaattattg gctggagctg gagaaatctt tggttcagca agtgaaacct
    32161 tcgctatctt ggacaatgcg gatattgtgt tcccttatgt cctagacgat gatggtaaag
    32221 aagttcagct atctcatggg acttacacac gtttgatgga gtctaaaaaa cgtgaggttc
    32281 gccgtggtgc ctatcaagct ctttatgcga cttacgaaca attccaacac acctatgcca
    32341 aaaccttgca aaccaatgtt aaggtgcaaa attaccgtgc taaagttcgt aactacaaga
    32401 gtgctcgtca tgcagccctc gcagcgaatt ttgttccaga aagtgtttat gacaatttgg
    32461 tagcagcagt tcgcaagcat ttgccactct tacatcgcta tcttgagctt cgttcaaaaa
    32521 tcttggggat ttcagatctc aagatgtacg atgtttacac accgctttca tctgttgaat
    32581 acagttttac ctaccaagaa gccttgaaaa aagcagaaga tgctttggca gtcttgggtg
    32641 aggattactt gagccgtgtt aaacgtgcct tcagcgagcg ttggattgat gtttacgaaa
    32701 atcaaggcaa gcgttcaggt gcctactctg gtggttctta cgataccaat gcctttatgc
    32761 ttctcaactg gcaggacaat ctggacaatc tctttactct tgttcatgaa acaggtcaca
    32821 gtatgcattc aagctatact cgtgaaactc agccttatgt ttacggagat tactctatct
    32881 ttttggctga gattgcctca actaccaatg aaaatatctt gacggagaaa ttattggaag
    32941 aagtggaaga cgacgcaaca cgttttgcta ttctcaataa cttcctagat ggtttccgtg
    33001 gaacagtttt ccgccaaact caatttgctg agtttgaaca cgccattcac caagcagatc
    33061 aaaatgggga ggtcttgaca agcgatttcc taaataaact ctacgcatac ttgaaccaag
    33121 agtattatgg tttgagtaag gaagacaatc ctgaaatcca atacgagtgg gctcgcattc
    33181 cacacttcta ctataactac tatgtatatc aatattcaac tggctttgcg gccgcctcag
    33241 ccttggctga aaaaattgtc catggtagtc aagaagaccg tgaccgctat atcgactacc
    33301 tcaaggcagg taagtcggac tatccactta atgtcatgag aaaagctggt gttgatatgg
    33361 agaaggaaga ctacctcaac gatgcctttg cagtctttga acgccgttta aatgagtttg
    33421 aagcccttgt tgaaaaatta ggattggcat aaaatggttg aatcgtatag taagaatgct
    33481 aaccataaca tgcgtcgtcc tgtcgtcaaa gaagaaattg tagacttgat gcgtcagcgt
    33541 caaaagcaag tcacaggttt cttgaaagaa ttggaagact ttgcccgcaa ggaaaatatt
    33601 cctattattc cccatgaaac ggttgcttat ttccgttttc ttatggaaac catgcagcct
    33661 aaaaatattc tggaaattgg gacggctatc ggtttttcag ctctcttgat ggctgaacat
    33721 gcgccaaatg ctaagattac aactattgat cgtaatccag aaatgattgg ttttgccaag
    33781 gaaaattttg cccagtttga cagtcgcaag caaatcactc tcctagaggg agatgcggtg
    33841 gatgtcttat ctacactgac agagtcttat gatttcgtct ttatggattc tgccaagtct
    33901 aaatacatcg tctttctgcc agaaatcctc aaacatttgg aagttggtgg tgtggttgtc
    33961 ttggatgata tttttcaagg tggtgatgtt gccaaggata ttatggaagt ccgtcgtggt
    34021 cagcgaacca tttatcgagg ccttcaaaaa ttatttgatg caaccttaga caatccagaa
    34081 ctcaccgcaa cattagtgcc tttaggagat ggtattctca tgcttcgtaa aaatgtagca
    34141 gatgttcaac tgtctgaaag cgaatgattt tcagaaaaat ttaagaaaaa atagtaaaat
    34201 agatagagta acacttatct caaaggagta gacatgaaga aaaaattatt ggcaggtgcc
    34261 atcacactat tatcagtagc aactttagca gcttgttcga aagggtcaga aggggcagac
    34321 cttatcagta tgaaagggga tgtcatcaca gaacatcaat tttatgagca agtgaaaaac
    34381 aacccttcag cccaacaagt cttgttaaat atgaccatcc aaaaagtttt tgaaaaacaa
    34441 tatggctcag agcttgatga taaagaggtt gatgatacta ttgccgaaga aaaaaaacaa
    34501 tatggcgaaa actaccaacg tgtcttgtca caagcaggta tgactcttga aacacgtaaa
    34561 gctcaaattc gtacaagtaa attagttgag ttggcagtta agaaggtagc agaagctgaa
    34621 ttgacagatg aagcctataa gaaagccttt gatgagtaca ctccagatgt aacggctcaa
    34681 atcatccgtc ttaataatga agataaggcc aaagaagttc tcgaaaaagc caaggcagaa
    34741 ggtgctgatt ttgctcaatt agccaaagat aattcaactg atgaaaaaac aaaagaaaat
    34801 ggtggagaaa ttacctttga ttctgcttca acagaagtac ctgagcaagt caaaaaagcc
    34861 gctttcgctt tagatgtgga tggtgtttct gatgtgatta cagcaactgg cacacaagcc
    34921 tacagtagcc aatattacat tgtaaaactc actaagaaaa cagaaaaatc atctaatatt
    34981 gatgactaca aagaaaaatt aaaaactgtt atcttgactc aaaaacaaaa tgattcaaca
    35041 tttgttcaaa gcattatcgg aaaagaattg caagcagcta atattaaggt taaggaccaa
    35101 gccttccaaa atatctttac ccaatatatc ggtggtggag attcaagctc aagcagtagt
    35161 acatcaaacg aatagtccaa atcaatgagt cagggaaaaa actcgacttc aggaaaaaat
    35221 gaagcaaaca ttcccacaat aaaacgcata gtacaaggtt tgtactgccc cccaaaaaag
    35281 ttagacaatt aatttatccg aaggatttag ttctgtattg cacagagcta agtcctttta
    35341 gttttatctt aattctctt
//
LOCUS       DAVKGC010000009        31929 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKGC010000009 DAVKGC010000000
VERSION     DAVKGC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31929
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.009"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(356..2083)
                     /gene="ezrA"
                     /locus_tag="VW049_000363"
     CDS             complement(356..2083)
                     /gene="ezrA"
                     /locus_tag="VW049_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9431545.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2165..4111)
                     /gene="gyrB"
                     /locus_tag="VW049_000364"
     CDS             complement(2165..4111)
                     /gene="gyrB"
                     /locus_tag="VW049_000364"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9431546.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4126..4689)
                     /locus_tag="VW049_000365"
     CDS             complement(4126..4689)
                     /locus_tag="VW049_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9431547.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQT
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4774..5328)
                     /locus_tag="VW049_000366"
     CDS             complement(4774..5328)
                     /locus_tag="VW049_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9431548.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5339..6562)
                     /locus_tag="VW049_000367"
     CDS             complement(5339..6562)
                     /locus_tag="VW049_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9431549.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6599..9049)
                     /locus_tag="VW049_000368"
     CDS             complement(6599..9049)
                     /locus_tag="VW049_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9431550.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDVHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSCRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATIK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9249..10178)
                     /locus_tag="VW049_000369"
     CDS             complement(9249..10178)
                     /locus_tag="VW049_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9431551.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNWIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10318..10605
                     /locus_tag="VW049_000370"
     CDS             10318..10605
                     /locus_tag="VW049_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9431552.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10639..11973)
                     /gene="ciaH"
                     /locus_tag="VW049_000371"
     CDS             complement(10639..11973)
                     /gene="ciaH"
                     /locus_tag="VW049_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9431553.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11963..12637)
                     /gene="ciaR"
                     /locus_tag="VW049_000372"
     CDS             complement(11963..12637)
                     /gene="ciaR"
                     /locus_tag="VW049_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9431554.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12746..15292)
                     /locus_tag="VW049_000373"
     CDS             complement(12746..15292)
                     /locus_tag="VW049_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9431555.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLALRFDQAAGELALSNMP
                     EIDVDNRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15415..15855)
                     /locus_tag="VW049_000374"
     CDS             complement(15415..15855)
                     /locus_tag="VW049_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9431556.1"
                     /translation="MTPQEMWNKYKQINPSIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVKPFNRVSADHAYKEGE
                     GNKTLVYWRQVHEDFFRDCLGEAGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15830..16477)
                     /locus_tag="VW049_000375"
     CDS             complement(15830..16477)
                     /locus_tag="VW049_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9431557.1"
                     /translation="MKNQLALSGEKILEKIYPLFGYRGTRRLLACLDEFQAEAHVVTE
                     VYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIELPSAYFDLSSILETGI
                     SKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKARKNKINFAVAGYLNTS
                     FIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKKLQRSTHDTARNVE"
     gene            complement(16455..16955)
                     /locus_tag="VW049_000376"
     CDS             complement(16455..16955)
                     /locus_tag="VW049_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9431558.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(16966..17664)
                     /locus_tag="VW049_000377"
     CDS             complement(16966..17664)
                     /locus_tag="VW049_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9431559.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     RFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17657..17923)
                     /locus_tag="VW049_000378"
     CDS             complement(17657..17923)
                     /locus_tag="VW049_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9431560.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(17989..18915)
                     /locus_tag="VW049_000379"
     CDS             complement(17989..18915)
                     /locus_tag="VW049_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9431561.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(18990..19805)
                     /locus_tag="VW049_000380"
     CDS             complement(18990..19805)
                     /locus_tag="VW049_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431562.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPSAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(19809..20129)
                     /locus_tag="VW049_000381"
     CDS             complement(19809..20129)
                     /locus_tag="VW049_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9431563.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMCPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20550..22547)
                     /gene="metG"
                     /locus_tag="VW049_000382"
     CDS             complement(20550..22547)
                     /gene="metG"
                     /locus_tag="VW049_000382"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9431564.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGTILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22671..23930)
                     /locus_tag="VW049_000383"
     CDS             complement(22671..23930)
                     /locus_tag="VW049_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9431565.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESL
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(23932..24633)
                     /locus_tag="VW049_000384"
     CDS             complement(23932..24633)
                     /locus_tag="VW049_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431566.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24617..25816)
                     /locus_tag="VW049_000385"
     CDS             complement(24617..25816)
                     /locus_tag="VW049_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9431567.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PIHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26081..27427
                     /gene="gor"
                     /locus_tag="VW049_000386"
     CDS             26081..27427
                     /gene="gor"
                     /locus_tag="VW049_000386"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9431568.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNRESYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAASEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
     gene            27624..28160
                     /locus_tag="VW049_000387"
     CDS             27624..28160
                     /locus_tag="VW049_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9431569.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            28211..28297
                     /locus_tag="VW049_000388"
                     /pseudo
     CDS             28211..28297
                     /locus_tag="VW049_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(28411..28626)
                     /locus_tag="VW049_000389"
     CDS             complement(28411..28626)
                     /locus_tag="VW049_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9431570.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            28724..29044
                     /locus_tag="VW049_000390"
     CDS             28724..29044
                     /locus_tag="VW049_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431571.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29075..29413)
                     /locus_tag="VW049_000391"
     CDS             complement(29075..29413)
                     /locus_tag="VW049_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431572.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29425..30144)
                     /gene="trmD"
                     /locus_tag="VW049_000392"
     CDS             complement(29425..30144)
                     /gene="trmD"
                     /locus_tag="VW049_000392"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9431573.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30134..30652)
                     /gene="rimM"
                     /locus_tag="VW049_000393"
     CDS             complement(30134..30652)
                     /gene="rimM"
                     /locus_tag="VW049_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9431574.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(30683..31471)
                     /locus_tag="VW049_000394"
     CDS             complement(30683..31471)
                     /locus_tag="VW049_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9431575.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELLE"
BASE COUNT         9430 a         7033 c         5722 g         9744 t
ORIGIN      
        1 aactatccgt gagttctcta gtctggagat ttttcaatag acttcgttat tggacggtta
       61 ccaagttact caaagtacag atttgaagct gcagataaga ctgacgaaat cactttaata
      121 ctaaatgaaa atcaaaaagc aaactaagaa gctagcctca ggctgtactt gagtacggta
      181 aggcgacgct gacgtgattt gaagagattt tcgaagagta taaaacactg ttttgaggtg
      241 gtggataaat cggctgggta aaaactcaat ctcaggagaa aatgaagtaa atctccccac
      301 aactatagaa aaagatttga ttctgctcct cacacaataa aatctttttc ttttattaaa
      361 aacgaatcgt ttcacgtgtt ttctcatatg aggtaacaaa gcgattggtt acaccaggct
      421 ctgccacttc caatgcttga gaaatcttgt caaatgaagc gtgataatca aattcttttt
      481 caaaaatatc taaagcttcg ttaaatgctt cttgaatgcg ttcatcaaat gagcgatagc
      541 ggttagaata ttgcaagagt tgctctgtca aagttgcata ttgtacaata ttataagttt
      601 ccgtttctaa agcttccata tcattcgttg caatttcaag aactcgggta acagattcaa
      661 tgttaatcat tttttgttct aactcaacca ttaaatcctc ggtattattg cttgccgtaa
      721 agaataactt caagaaagtt tgtggaatac ctggcagatt gcgtttttcc atgtatcgct
      781 tgatagtatg gagacgattg acataaacat tggccttttg acgtgcatta atatcatctt
      841 tctcaatttg tgtcaggcgc tcactaactg aaatttgctc atcttcaata tcttttagtt
      901 gagtttgtaa atcctcaaga ttttcttcaa gaactgaata agcttgggtt ggttcttctt
      961 gatttgaagt tacctcaaca atagctgcct caaaactctc taattctgtc tgaatacgac
     1021 gaacatggct tgcagctgtc tcaggaagta aataggtctt gttcaaacgt gcaatatctt
     1081 ctcccaataa agtattattc tctttcatat gttgaaggta agttggaaga gttgcaagta
     1141 gattttccac tactttctga gcagcaattt ctcgagtaaa aatatcatac aaggcattga
     1201 tttcctcttg tgcctgtcca ttctcatatt cggcattatc caattccaac tgacgaatat
     1261 tctcttggtt tttcttgaat gcttcataaa gcaagtggaa acgcgcttca atatccgttt
     1321 caacaaaatg ataattagca tcaattagtt tacgataacc ggattccaaa tcctgtaatt
     1381 gatctggcaa ttctgtagaa agcgtcgtaa ccaaggctgg aacacgatcc acaatatgac
     1441 ttaaggccaa aatgtgattt tctgtattat ccaaaatcac tgcggcttcc acagggtcac
     1501 ccgatgaatt caaggttaca aattgtgaaa attcagattg gatattttct aattgttttt
     1561 caatttcatc caaggcttga ccatactgtt ctgaattttc agcaactcta tgctgaagtt
     1621 cctcaaataa atccaaagca tgaagaacac gaccactatt tttagattct tgcttctcta
     1681 agtctgccaa agcattgcga attgccgcaa tatcttcttc aatcaaagta atttgactct
     1741 caatttggtc aatttgatga ctggccttga gaaaacgaaa tgaatggtta taaccttctg
     1801 cttcaaagag attattttca atatcggcaa aagagttgag agataaatcg acccattttt
     1861 gattccattc acggaaagcc acttgacttt gtccaatcaa gtgcatattt tttacagctt
     1921 ctacttcatc atttactgga agattgtata gttcttcttt tctttcttct agcgcctcta
     1981 atctcccctc gtttcgctta cgtagaaaga ttgccactac ataagccaga actaaaatga
     2041 ctgcaattgc aaccattaaa taaattagtt gtccattaga catatcaaac tcctttttta
     2101 cttgaaacaa tcgtaacgat tatatcatat tttttagacc aagggaacta cttctcccta
     2161 ttttttagac atcaagtgta ctatagacag cattttcttc gataaactca cgacgaggct
     2221 ctactcgatc ccccatcaac atatcaaaga ttttatctgc ttctgcagca tcatctacag
     2281 aaactctagc catcaagcga tgttcgggat ccatggttgt ttcccacagc tgatgatcgt
     2341 ccatttcacc tagcccctta taacgctgaa tagtcggttt ggtacgacct tcactataac
     2401 gggctaaagc ttcttggagt ttgatttctt gatctgcacc cggctggata tattctttaa
     2461 tctcgctccc aaccttgaca ccatagattg gtggttgggc aatataaaca taaccagctt
     2521 ctaggattgg tttcatataa cgataaatca aggttaaaag aagggtacga atatgggctc
     2581 catcgacatc ggcatcggtc atcaaaacga gtttttggta acgggctttc gaaacatcaa
     2641 attctgcgcc aaatcctgtt cccatggctg tgaaaagact acgaatttct tcgttagcta
     2701 gaatcttatc catacttgct ttttcaacgt tcaaaatctt accgcgaatt ggaaggatag
     2761 cctgaaactc acggttacga ccagatttgg ctgatccacc agctgagtct ccttcgacga
     2821 tgaagagttc tgtttcagca gggttattag aagaacagtc tgctagtttc cctggaaggt
     2881 tggaaatttc caaaccagat tttttacgtg tgacttcacg cgcacgcttg gcagccacac
     2941 gagccttggc agctaaaatc cctttttcca cgatacgttt ggcaatctgt ggattttcca
     3001 tgaggaaatc ggagaaggct tcactgaaga ggcgattggt aatcttgacc acttcgctat
     3061 ttcccaattt ggtcttggtt tgtccttcaa actgtggatt tgggtgttta actgagataa
     3121 ctgcagttaa tccttcgcga acatcttccc ctgttaaatt atcttcattg tctttcagta
     3181 acttattctt acgagcataa tcattgataa cacgagtcag tgccgtacgg aaaccttgtt
     3241 catgcgttcc accttcatgg gtatgaatat tattggcgaa actcatgaca ttttcatggt
     3301 aacctgttgt gtactgcatg gctacctcaa ctgtaatatc atccatctca ccgtctgtat
     3361 agattggtgt atcaaagatt acatccttgt tctcgttgat atattcaacg taactagcaa
     3421 tcccaccttc ataatgataa tgcttggttt gttccaaacc ttggcgctta tcagtgatag
     3481 aaatttgaag accgcgattt agaaaggcca actcttgaat ccgtttattt aatttatcaa
     3541 aatcaaaggt tgttgtttca gtaaagattt ttgggtccgg tataaagtga accgttgttc
     3601 cagttctatc tgtatcccca accacctcaa ggtctgcaac aacatgacca cgacggtatt
     3661 cttggtaatg aatcttaccg tttttatgga catgaacgtc taattgagtg gaaagggcat
     3721 taactactga cgaccccacc ccgtgaagac cacctgaaac cttgtatcca ccaccgccga
     3781 actttcctcc agcatgaagg actgtaaaga cggtctcaac agcaggacgg cctgtttttt
     3841 cctgaatatc gactgggata ccacgcccat catccacaac agtaatcgaa tcatctggct
     3901 caataaaaac ttgaatatgg ctggcaaatc ctgccaaggc ctcgtcaatt gagttatcaa
     3961 caatttccca gactagatgg tgaagacctt cttttgaggt tgatccaatg tacatccctg
     4021 gacgcatacg aacagcctct aagccctcta aaacttgaat ttgactggca tcataatcct
     4081 gtgcctgcag atttttgatt tcttctgtca tcttattcct ttttcttata tgtctaatac
     4141 ttgtcttaaa ttcacgatac tggtaaacaa gtgggtatca agtcctgcag cttgacctgc
     4201 ttcgatatca atcggccgat caccaatgac aagaccagag ctaatctgat acttttctct
     4261 taaataaagc atggattcgg gatttggctt tctcttaaag cctgagctag aagtcaccac
     4321 ttctgtaaaa taagctgcta tagaggtttt ttctaaaatt tccaaaacct gatcatttcg
     4381 atgagagacc aaaaaatgac ggccaccttg atttaaaatg tcttccaata ggtcagaaac
     4441 tccttcaaat aaaatcgggt gttcaagctc tctggcttca ttttccttgt acttttctaa
     4501 aaaattctct aaattgggag cgaatgtctc aatcgcaaaa ggagtagaaa cctttaaagt
     4561 ttgatagaca ctgtcatggt cttgtgtgat accatacagt gccaatgttt caacaaatgc
     4621 agctgttgaa gtttcataat tatccagtaa agttccacct aaatcccaga tgtaatcgtg
     4681 atatttcata cccttcatta tagcattttt ttatgaaaaa agcgatgatt tccctgagtt
     4741 ttccacataa aaaacgagag aataactccc gttttactga tttttaccaa agacatctcg
     4801 atagagcatt cctgttcgta aactctctgc gtcccctcct agttgctcca ccaactcgta
     4861 ggcaaaggca agggctgttg aaggaccccg actggttgtc aactgaccat ctaccactac
     4921 tgtttccttg acgtagtgac catcaaggat ttgctcttga acgccgtcat aacaagtgta
     4981 tcgcttattt ttcaatatct ctgcttgatt gagggcaatt ggtgccgcac aaatggctgc
     5041 tagtttcttc ccttcttgtt cgaagctttg caattcttga atcaaggtct gattatcacg
     5101 taaatgtgca gaaccaggca tacctccagg aagaacaatc atatcatagt ctgataaatc
     5161 tccatcaaag acatgatctg ctcttacttg gattgcatgc gaacccgtta cttgctcttc
     5221 aaaaccaacc atatcacatg tgatattggc tcgacgcaag acatctacaa ctgtcaaggc
     5281 ttcaatttct tcaaagccct gagctaacat aactgctact tttaccatga ttttctcctt
     5341 atttaatttg ttttaataca acctttttcc gtttgaatgg gacttttccg ctcttttctt
     5401 cagctagatt agtctggtaa ctcatcgata aaagcaaacc aacaccaatc agattactga
     5461 taatagctga tcccccttgc gaaatgaaag gcaagggaat ccccgtcaaa ggaagtagtc
     5521 cagtcacagc accgatattc tcaaagatgt ggaagagcaa catcataatc aaacctgtgg
     5581 aaatataagt gtagaactgg ttatttgatt taagagtaat cttcaacata cggtaaatca
     5641 acatgagata gagggcaata accaggacag agccaataaa gccaaaatct tctgcaataa
     5701 ccgtaaaaat catatctgac tctcgaactg ggataagcag attcgaagca ttaaatccct
     5761 gaccaaataa gccaccactc ccaatggcaa tctgcccttg agcctgctgg taagtcgttg
     5821 tttgggcaaa ctcaaaggga ttgagccaag ccaaaatccg attgatttgg taggtcggca
     5881 ttccaatctg gtgaagaaaa gctcgtccgt ccttgctaat aaagatagct aagaaaccag
     5941 caactcctgt tacagcagtc acaaatactg ggataataat tttccaagaa acccctgata
     6001 ataaaacgat tcctgagaaa atggctacaa aaaccaaagc cgtccccaag tcactttgaa
     6061 gtgctaaaag aactaggact ggaatggtaa agagaatcat ccagaaaatt aacaaaaagt
     6121 ccagcgaaac cgtgcgtctc cattccttat gtttctttgt aaattggaca atgacacgag
     6181 ccaacatgag gatataggat atcttcataa attctgacgg ttgaaatagg gtaattccat
     6241 ttattgatac ccagtttttg gcacccgttg atgcaactaa gcttggatta taaaatacaa
     6301 tcggcaagat cataagtccc aagcctaaaa tatatagaaa gggggtcacc ttccaaagaa
     6361 attctgtatt aaagagcatg accacaaaac caatcacaag ccccaaggca atccaggcga
     6421 cctgctgccc taaaatgggc agaatattat tgggataatc atgactaacg gctatataga
     6481 tagccaccac accgatgacc agtagaaaaa atactggcaa gagcaaacta taatcaactc
     6541 tagagtcgag agaacgtttc atatatacta accttatact ttcatacaat actatttatc
     6601 aaagttcatt aaaaaatcta tcaatagcct cgtcaacttc ggatcgagag atggttttaa
     6661 tagtcgcttc ttctgctaga gatgctacta tttgtttgcc gtatcgtttt ccgatgattc
     6721 tcctatccaa aataagagtt aaggaacgtt ggtattcacg tctcatactt cttcccaaag
     6781 cctgttttaa acgaataatg gccattggca attgataatc ataaaaggca tttttccctt
     6841 cttgattcag ttcttgatta atctttttcg tcaagggttc ttgaggattt tggaaaggaa
     6901 gcctcggtac aacttgaatc acaaaaggat ggcttgaaaa atcaactccc tcccagaaac
     6961 ttgctgcacc aagcaagatt tgttgttcac ctttttcaaa gcgtttcttt agctgatgaa
     7021 catccccatt tttatactgg gccaagtggc taactgtaag taaatccgat actgctagaa
     7081 gcatgtcttt agcggtaaag agaaccaaaa tcggttgctg gaaagcttga atttccacta
     7141 gtaaagcagc tacctctctg gcatagactt ctaaggaggt ttctgttacc agagggaaat
     7201 ctttgaccat aaccacttct tgttcctgtt ttccccgaga ttcaatcttg acaaatttgg
     7261 cttcaggata gcctaaaagg tctgccaaag aaaccctctg actaatctca agagtagccg
     7321 atactcccaa gacttggcaa ctctcaggaa gtaaagagga cagaagagta cggcatgatt
     7381 ttgtagaaga aatctgaatt ttcttttgac tcgtttcagt tacttcaagc cagtaatcac
     7441 cttcagctgt aaaatagcga accagctcat caaagccttc tacttctaat tctgaaaagt
     7501 actgatggag attgtccaga gaatctaaga tatttttcct agatttacca gactgaaatt
     7561 gttctatcaa gtagagacac tcaaagcgaa tactttctag tatccgttgt tgaaccctgt
     7621 tttcttctcc tactaaagcc ttatcaatta aatcgataat agattgtata tcgtaggtct
     7681 cttgaagcag attttctaga gctaacaaaa tcttttggac ttcatcaata atcagtaaac
     7741 ggtcactgac aaattcagga ttatcttcaa gtctggtcac gagataggca tgattagtca
     7801 ctaaaagctt gcaagtctct gccctttctt gactacgttt ccaaaaatct tccgtcacaa
     7861 ataagctctg ggatgataaa ttcccatcat gacgaaggtc tgctagaaaa tgttggtaac
     7921 ggtagagttg cccgatttca tccaaatctc ctgtctctgt ctcagtaagc cagaccaaga
     7981 cttgcatttt aaagcgtcta aataagcgat tttcatcatt ttcctgcaag gaatgataaa
     8041 aggcatccaa cttcagataa ttttgtggtc cctttaagct atgaatatct gtatggaaca
     8101 cttccttgag gcgtttacct tcttcttcca tgatttgatt ttgaagaatc tttgtcggaa
     8161 cactaagaac aatttgtcgc tctttggatt gagataaagc gggtaagaga tagccatagg
     8221 ttttcccaat ccctgtcggc gcttgaatca gagagacagg ttcatctttc aatagcaagc
     8281 caacctcttt agcaaaactt tcttgttcct ccctcacttc aaggttcaac agagaaatat
     8341 ttttagaaaa gtcttgagat agttttcgtg gctccagaga agctgccgtt ttcttaaaat
     8401 acagaccttg aacttggacc aagtctggag aactcaggat agattggttg cgataagttt
     8461 cctcaataac caggtaggac tcatatagga gagcgtcagc catttccagc aagcgttcca
     8521 agagaccttt aggaagctga gtcatctttt cccgtaaaaa aagaagtaat tctgctgtag
     8581 cttgggcatc tgaaagggct gtgtgtgcgt gtttaagagg aattcctaat tctcgacaca
     8641 aaatcggcaa gctatatttt tccagttcag ggaaaaagac ctgggccaat tcgaccgtat
     8701 caacacgagg gtttcttagc tcatagcctt caaaaaataa attttccgcc aagagattag
     8761 catcaaactg aacattatgg gctacaaaaa tcccatcctc caccaagtca aatatttttc
     8821 tggcaacttg cgaaaaatca ggtgcttgcg ccagacgttg gtctgtcaat cctgtcagtt
     8881 ctttgatatg aacatccaag ggttcatgtg gattgacatc cgtcgtatag tgatcgacga
     8941 tttctccgtc ctcaatcacg acaattccca cttggataat tttagcctta ctacctgtgc
     9001 tagttgcctc taaatccacc acaacatagc gattaccaat cgttttcatt ataaaaaatt
     9061 ataccaaaaa aagggccatt tggcacctgc aaacatagga taattttcaa actcaagaac
     9121 actacttttc ttaaaaatcc catcgaatag agcattctag cctgctgatt tttgtaaaat
     9181 agtgaagaaa tacgaaaaat tgagagaaga aatgtttgca tcttccttct ctcaacgaat
     9241 catgtatttt atctaaccat gtcaggatcg tagtcataaa atcctgtatc aaggaaacgg
     9301 tttttagggt gaagtttacg aacttcttca tccagcaaga aggcttgatc atggctaaag
     9361 ttgccctctt gaatccaatt acgcgcttgg ataaagagtt ttgcaatctc aacaaagttc
     9421 tgaccaggag tgtagagccc ttctagtttc cagcgagtaa agccatgctc caccaattct
     9481 gttaatttga tcatcaaatc aaggtcattg ttggcaaaga tatgggtccc atgattatct
     9541 tcaaaaatgg aatagtggct ctctggatca cttggctcag ccaagaagag gtcacgttta
     9601 tgcgtctttt catcatcgat atgtgtaaag ttatagtagt tttgcaagag tggacgttta
     9661 gaatgatgga tgacgctagc accgtaaacc aaaacttcag caggaatttc caaaatctct
     9721 ggcattttga aaagttcagc tgatggaatt tcacgcgcca aaacagcctc agatgcgcca
     9781 gccttttgtc cccagaagtt aatctgacga ctgctagtta ccatggttga agcatcgtag
     9841 atggtcttaa atgaataacc atcgcggtta actacgtaaa agacgcctgc atccccaatc
     9901 gtaatatagt ctgtcttgat ttcttccaag aagtctaaga aaggcttgat acggtccatc
     9961 atatcttggt gcatgagagc attgaccgca acgagcaatt ccttaccagc atcatgaacc
    10021 aacttagcga tttcacgtaa ttggtcataa ctaaaggtcg ttggcagacg aaggccaaaa
    10081 tctttctcac cgacatagat acggtctacg ccagcttcga gtagttgttc aacttgttca
    10141 atactttcag cagttgctgt aatgacaatc ttttccataa gaaaaattat accacatttc
    10201 tccaaaatca gccatgcttt gaaaatgaaa agggacttta ttctttttgt aacctttctg
    10261 taataattct ctgccgaaaa aatgataaaa taggtatgta ctgttaagga gagaattatg
    10321 cccgtaagaa aattacaatc ctatgaggta gactatcaag aagaattaaa ccagcagctt
    10381 cctcattacc aagcttatac acctgaagcg caatctgatg ccaatctcaa ggaaatccta
    10441 ttttttatta atatcgctgt tttttgtatc tgtattgcta tctttagttt tatcttttta
    10501 gcattaaaat tatcaactgc tcttgccttt gccgcagcaa tcggattcag cttacttgtt
    10561 ttaaaagttc aacggtctat tatcaaacga aaacgtagaa gataaatcca aaatatcgcc
    10621 ccagttggag cgatattttt attttttctt tttagatggt gtctgaatgg caatcttcac
    10681 ttcaaagatt gttcccttgg gtttattatc tttgacagta acagttcctt ttagagcatc
    10741 tacaatttgc ttggctaggg ataatcctaa accaaaacca cctttttgcc gagttctagc
    10801 cttgtctact cgataaaaac ggtcaaaaat tttcttttta tcttctgtcg aaataccgat
    10861 tccattatca gaaacaagta aataaagatt gcgatcggtc gccgagataa gaaaatcaat
    10921 ttcaccatcc tcctcagtat acttgacggc attatcgaaa agaatggtca tcagttgttt
    10981 cagaagaagc tgatctgtga caattgttcg atggatacga ttttcaaaac ggaagacacg
    11041 attattttcc gaagcaatca tctcatagtt tgtgaaagtt gtattaaaaa agctagttgg
    11101 aacttctgca agctccggct taatcccatc atctctccga gctaagttca gcaagcttgt
    11161 cgttaaaaaa cgcatatttc ggacttcttc caaactcgat gcaatgcttt cgctcacatc
    11221 cataatggta gcttctggct tacgaaaaag ggtctctaag cgattttgca aaactgcgag
    11281 tggagttcgt aactcatgac tggcattttc cacaaaagac tgttgcttct gcatactctc
    11341 aagcaggggc ctaacactga ccctagctag atagagactg gcaagtaaag acaaaatcca
    11401 gaaactagcc atcacgacca caatcaattg ctcatgcttt tgactagcct gctccaactg
    11461 actggtatta atcaagacag cagcatactt gatattggtt gaaaccgaac taatattggt
    11521 ctccatcaaa atcacacgat agatttcttc ctgcccatag ctattaaaaa cctgaatctg
    11581 gtagatatgt cctagttctt tcttttctaa cttaatctta tccaagccca aaaatcgatt
    11641 tccagaaaga agttgagtaa agtccttatc aaagagaata acttccgtat tggaactgac
    11701 attaggtttt atttctactt tactagcgtc cgccctagca ttttctaaat ctttaatctc
    11761 ttctgttgcc ctatttatag ccagctgaat aactgcttga ggattttcac tcagtccatg
    11821 aagcttatcg tccaccgaag tataaagact cgaatgcatg acttgtaaaa taatcagagt
    11881 cattgtagaa aaaatcaggg tgaagacacc gaagttgcgg ataaaataac taaagtcatc
    11941 cgcataccat gtttttttaa gtttactgaa catcttttaa aagataccca acactacgca
    12001 aggtttgcaa attctctgca aaagtggttc cctttaattt cttacggact tttgaaacat
    12061 agacttcgac aaccgaaatc gttgtatcac tatcaaatcc ccatagacgg tcaaaaatct
    12121 gcgtcttagg caaaatcaca ttttgatttt gaaggaaata aactagtaaa tcgaactctt
    12181 tccccagcaa ttcgacagga gtatcttcaa ctttaacggt attggttgat aaattaacca
    12241 cgatattccc ataagtcaag gtgttttcat taaacttccc tgaacgtttg agaagggcct
    12301 gaatccgcat tttaagttct tctaggtaga aaggtttggt cagataatca tccgctccca
    12361 gttcaaatcc atgtcccttg tcatccaaac tttccttggc agtcataatc agaactggtg
    12421 tcgtaattcc cttttcacgc aattctttta agacttggaa accatttttt tctggcaaca
    12481 tcaaatccag caaaatcaaa tcatagacac cactctcagc ttcgtagaga ccttcttctc
    12541 catcaaatac ctgcataaca tccgcaaaat cgtctaaaaa gtcaaatact gaatttgaca
    12601 gacctaggtc atcctcaacc aataagattt ttatcatgag aaactcctcc ttattaaaac
    12661 tattatacca aatttgcctt aaaaaaaact caactctctg cattttacat gagatagctg
    12721 agttttcttt ttattttagg cttatttatg catttccgta ttgaagaaca actgcttcga
    12781 ctgcagcttt ttcacggcta atcaagtcaa cacgtgctgc aatttccttg attcccatac
    12841 cgatgttacg gctaagagca aggtcagaaa gttgcggctc aaagaactcc ttgtattccg
    12901 ccaagcgttg ctgagtctta aatacatgag caggaaggat aacaaagcta tcaaagctca
    12961 tatctcctcc aagggctgcc ttaatccaag cccagttttc acgcgcccaa gaccaagctg
    13021 ttttctgagt tgcttgatga gctaggaatt ggtaatacca agcagacaag tcctgtggtt
    13081 tgaccacaaa tttgtccttc caagaagtaa tcaagttttg gatattatcc gcatctgtac
    13141 tgtatgcaag agctgctgcc aactggcgtt taaagacagc atctgttgcg tgagtataag
    13201 tatcaagata aagtgctaac aagtctttag tctcatgatg tttcatctca ttaatcagaa
    13261 cttgtgagcg aatagctgct gggagtcctg caagattctc cttgtgtgtt gcgaagattt
    13321 ggctagcgac ttgactagct tctgcatcat ttgagcgaat catcatcgaa acagccagct
    13381 gacgaaccaa ttcatcctca tctgattctc cgtctttagc ttcaaaacca agacggtcat
    13441 agttatgacg agccaattta gcaaccagtc ctttgaaggc tgtttcagca tccgttcctt
    13501 catcaataaa gcgctcaagg gctgaaatca cttgagaaac agctgaaacc accagataag
    13561 actcttcctt agcaagttta tcaagaactg gaagcaagtc tgcataagaa atgtgccctg
    13621 cctcagccaa caaacgacgt tcttgaacaa tttgcagttt gcttgtgtta tcaagtgtct
    13681 ctagctcagc aagaacagct tctaacaagt ctccttgata gtcggtaata tagtgggcag
    13741 tattttcagt gttgagacga agagctcctt cattctcagc aagaagagct gcgtagccag
    13801 ggatttcgat actttcagtt tcgagtgtat caggcaagcc tttccagttg ctattgaggg
    13861 gcaccaccca gagacggttc ttgtcttcgt tctcaccgat gaagaattgt ttttgtgaaa
    13921 tcttcaagac atcattttca actttgacag taagaactgg gtaaccaggc tgttccaacc
    13981 aagaatccat gaaggctgcg acatcacgtc ctgacgcttg accaagggca tcccaaaggt
    14041 cactaccaat ggtgttgctg tattggtgtt tttcaaagta ggcgtgcaaa cctttagcaa
    14101 aatcagcatc tcctagccaa cggcgaagca tgtgcatgag acggcttcct ttggcataga
    14161 cgatagcgcc gtcaaagagt gtattaattt catctggatg tttaacttcg acgtggacag
    14221 actgaacgcc atcagtagcg tcacgttcaa gagcaagagg tactccacct gtttggaaat
    14281 cttcaaagat attccagctt ggttcgatgg tatccacaca gacgtattcc atcatattag
    14341 cgaaactttc attgagccaa aggtcatccc accatttcat agtcacgagg ttcccaaacc
    14401 attggtgagc caattcatgg gccacaacaa gggcaacttg ttgacggcta gcaaatgtag
    14461 agttctcatc gacaaccaag taaacttcac ggtaggtcac aagaccccag ttttccatag
    14521 caccagctga gaagtcagga agggcgatgt ggagagattg agggattggg tatttaacac
    14581 cgtagtaatc ttcgtaaaac tcgatagagc gaacagcgat atccagtgag aaatcaagat
    14641 ttgaaagtgg atgtgctttg gttgagtaga cacctaccag ggtaccgttt ttagttttag
    14701 ccgtcacacc ttgcaaatca ccagctacaa aggccaacaa gtaagatgac atacgaggtg
    14761 ttgtctcaaa cttccaaata cctgtttcct tgcggttatc cacatcaatc tcaggcatgt
    14821 ttgacaaggc caactcacct gctgcttggt caaagcgaag agcgagatca aaggttgctt
    14881 tggcttctgg ctcatccaca catgggaaag cttcgcgcgc aaaatggctc tcgaactgag
    14941 tagacaagac ctccttcttg actccatcaa ctgtataata agaagggtaa atccctgtca
    15001 tgttgtctgt aattttacca gaaaaggcaa gaaccaattc aacttgacca gcctcagcca
    15061 attcgatatg aagggcttca ttgtcatggt caactgtaaa tggacgagct tgacctgcaa
    15121 cttctacaga ggtgatttcc aagtcttttt ggtggaggga gatgcggtca ctctgtgctt
    15181 gaccagtgat ggtcactttc ccagaaaaag tcttggtctc acgactcaaa tctaaaaata
    15241 aatcataatg ttcaggaaca aattgcttaa taaaatgttc aactgcttgc atagttttct
    15301 cctattctaa gtttaagagc tgctgctctt cttcaaacaa acttattata tcatattttg
    15361 cttgagaaaa aaggattgga cagcgatttc caaaactttc ttccttctaa tagtctacag
    15421 tgggtagacc ttgcgaaatt cttctaaaac aaccttgctt tcaggtgtaa aagtcaatcc
    15481 tgcttcaccc aagcagtctc tgaaaaaatc ctcatgaacc tggcgccaat agacaagtgt
    15541 cttgtttccc tcaccttcct tataggcatg gtcagctgac actcgattga agggtttaac
    15601 agaaaccttt gtaatttcga caatgcagac agcctgattt tgactatcta aaatgacatc
    15661 gaaggtccct acttggggaa gtggttcgtc ttctagtaca tagaggtcat aggctgatgc
    15721 tgttgctgtc ttttctcctt taaacaccaa atccgctaaa aggtctggtt caactccaaa
    15781 agcccaggca tcgatttcat ctccgataga aggattgatt tgtttgtatt tattccacat
    15841 ttcttgcggt gtcatgggtg ctcctttgta attttttact ttcttctttt atgtgtttaa
    15901 gatgatctgg atggtcaatc tctaaatcaa aaatctctgg aatagaactg tagtggataa
    15961 tgcacttgat acccaactga ttcatttttt gtatgaaaga agtattcaga tagcctgcta
    16021 cagcaaaatt aatcttgttc tttcttgctt tatcctgcat atctcttagc atatctaaca
    16081 ttattggact ttccagatca tgccattgac tgtttctcat agtcgcaaaa acaaaggaag
    16141 tcaaatcatt cattccaact acaatctttg aaatgcccgt ttccagtata ctagataagt
    16201 caaaatacgc tgacggtaat tcaatcatcg ttccgacttt cccagtaaag ccatactgac
    16261 gcaatactgt aatagcttgt tttaattggt cggcatcatt gacaaaagga aagataagag
    16321 atagattggg gttggtttga taaacttctg taacgacatg tgcttcagcc tgaaattcat
    16381 ccaaacacgc cagtaaacgc ctggttcctc tatagccaaa cagaggataa attttttcaa
    16441 gaattttttc gccactaagg gcaagttgat ttttcatcat tcaccttcca attcatgtaa
    16501 gaagtcttgt ccagttctgg aaatcctaat aattcagact taaccttcaa gactaatggc
    16561 gatgcatttt cttctgtaat ctcttgaata tccatccaaa tatatccaag tgaatcattc
    16621 gcaccatcag acacagcttc cgaagtcgta acttgaggtg cactctcatt catttcaaca
    16681 tcatacaagg ccatgacatg gtgaaccata aaatttgtta actcttctct gacgaaaaca
    16741 tcgtagattc gaggattaga gtagcttcta acagtaaatc ccgtctcttc cataacttct
    16801 ctagtcagcg tttccgtcag tccttcacca agttgctgac tgcctccagg tagatcatac
    16861 cgatgttgat aagggcctct cgttttttca atgcagagta acttcccatt ttctaagcaa
    16921 acagcgtaga ctccaaagtg ttttttgatt tccatccaac tcctcctact tcaaagacca
    16981 gccaccatct attgtcaaga tttgtccttg catggcgctc gcttttccac ttgctaaaaa
    17041 aagactaagc tctgctattt cctctggctc aatccagcgc ttgattggag tttcactagc
    17101 cacccagtca gccaaaccac ctggttcaaa atccgcagcg gtcatagctg tcttgactgc
    17161 tcctggagcg ataccaaaga cctgaatccc agcttcagca tagtctagag ccaactgctt
    17221 ggtgaagcca gccaaggcat gcttggatga agtataggcg tgaccacctc cacctgctag
    17281 gctagaagca atggaacaca tattgatgat gattcccttt ttattttcca gcatttgtgt
    17341 caaataatac cgagtcaact ctactggaat aatgtagttg atttcaaaaa tctcttgaat
    17401 gtcctgcgcc gtttgttcca acagtggttt gtaatcatcc aaaactccag cagtattaca
    17461 caaaacatcc acctgagggc accagtcaaa aataggttcc aagtccaagg tcaaatctct
    17521 ctgtaaaaag cgaaaatcac cctctaagag tggcttttca ccttggtcaa ctccataaac
    17581 ttgatagccc ttctctaaaa agaggcgagc ttgagccaat ccgatccctg aactcactcc
    17641 tgtaatgagt acacgtttag tcatgcactt ctacccaatc cgttgccaaa acatcacaaa
    17701 ctgtcgggct ccacatggaa aaaccttctc cttcgccaga aacgttgatt aggaaataag
    17761 gtgtcatttc aagtgcaagc ccattttgct cgatggtatc aaagagttgg acatagtttt
    17821 ccgcacctcc ccaaccagtt cgtacatatt ttctcttagc ctttaaccca ggcaggatct
    17881 cttcaaatgt catgtttttc tcctttaatt ctacattctt catttaatta tagcaaaaaa
    17941 ccgctttata cggctttttg aatgtgagtt attcaaacct gctactactt acggcaaatt
    18001 attccctgca gcaagataaa tttcatacca ttcttttctt gttaagctaa agtttgccgc
    18061 tcggctaact tctctcaagt gcttaggatt tgttgtacct acgactgcct gcatttttgc
    18121 tggataacgc aatatccaag aaatggcaat agttgaagag gttactccat atttaatagc
    18181 taaacgatca agtacttgat ttaaagcttg aaatttctca tttccaacaa aattcccttt
    18241 aaaatacccg aattgtaaga cagaccatgc ttgaatgacc acatcgtgta attggcaata
    18301 ttcaaaaatg ctgccatctc gcatagctgc ttgactatct tccatattaa catgaaaagc
    18361 tgattcaaat cctggagtaa aagccgcact caattgtagc tgattaacag ctaacggctg
    18421 cttgacatct tttttaagca actccatcat cataggattt tgattagaaa ctccaaaatc
    18481 tcgaacttta ccttgtttat aaaggagatt aaaggcttct gctacttggt cagattccat
    18541 caaagcatct ggtcgatgaa ggagcaagct atctagatga tcaatcttca atctttgcaa
    18601 aataccgtct actgatttta taatatagtc cttagaaaaa tcaaaatagg taaattcttc
    18661 aatgcgaatg ccacatttgg actgaatcca catcttttct cttaaatctg gacgattttt
    18721 taggacaaga cctaacagtt cttcacaacg accacgacca taaatatcag ccaagtcgaa
    18781 ggcattgatt ccaacagaaa gtgctgtttc tacaagctct tcaacttctt ttacagattt
    18841 atcttttatt ctcatcattc cgagaacaat ttctgataat tctttgtcat cttgaccaag
    18901 agttatgtat ctcatcaaat ttttctcctt taatttctaa cattcttccc ttcattataa
    18961 caaaaaaccg ctttgcaaca gctttttgac tatacttcac tccattttat cttcttaaac
    19021 ccacggaaca agacaaagat tccaataaag aggacagctg aaggaataac ttttgtaagg
    19081 aaaacatttg aaattcccat ccactcataa taacggagca gagaacccac cacaagatgg
    19141 gcaataatca tactgacaaa tggacgaaag accgcttctt tccaattcca aataccgata
    19201 actagcgaaa tcgtaaagac agacaaacta tcccagggag ccggaatata aaaggctcct
    19261 tcttgtatga agcttgccat tcctacatat cctaaaacaa ctagaagaac tatagtccca
    19321 acaacaatgt aagtgccaat tttcatttta ggagaatctt ggactaaact tcttcgtaaa
    19381 attgtggcca caagtccaaa tccaatcaga aaaataagaa gttgccctaa aaatgtgagc
    19441 aaattgactg ttaagagagg acctttagaa aaatcactta gtagttgata ataacgtaat
    19501 accgccagga caagaattgg cgtcaaaagg gactctttga tagaactgcg aggtgctccc
    19561 ttgagaatct ctttcattat ttttttagga ttcttaccta gataatcctc tgcactcatg
    19621 ccatctcgtt ctgcttctga gaaatctagc atcatcaaat agatctgctc tctgagatag
    19681 tcttcatcat agagaaatcc agcaagatta aaactttccc acaactcctc aaaatacttt
    19741 tgattctcct cagaaaactc atgtagcaaa gcgcttgttt cttcgtaata cttcattttc
    19801 ttcatggttt aacccccatt cttaatccct tctacttttt gactcaaatc gtcccattgt
    19861 tgccaaaaga ctgagacacg ctcttctcct tctttcatta atgaaaaata cttccgatct
    19921 ggaccatctg gcgacgggca catgtcgcct cttatccatt gatttttttc taacttttgc
    19981 aacaaaggat aaatagttcc tggaacgata gtatcaaatc cagcctctcg caaagtctga
    20041 accaactcat aaccataccg ctctttttga ccaatcatat ccaagacaca accttcaaga
    20101 acacctttta atagctgagt ttctttcatc acttctccct tctaatctat tttgtaatac
    20161 ctactagtaa cttcacctat agtatatcac ttctacacta gtttgtaaag cataatagtt
    20221 aatactcttc gaaaatctct tcaaaccacg tcatcgtcgc cctaccgtat gtctggttac
    20281 tgacttcgtc agtttcatct acaacctcaa aacagtgttt tgagctgact tcgtcagttt
    20341 catctacaac ctcaaaacag tgttttgagc tgacttcgtc agtttcatct acaacctcaa
    20401 aaccatgttt tgagctgact tcgtcagttc tatctacaac ctcaaagcag tgctttgatc
    20461 aacccgcggc tagcttccta gtttgctctt tgattttcat tgagtataaa taaaaaaaca
    20521 gaactagcct gaactagtcc tgtctacttt tacccaatca cacttccatt tggtacagct
    20581 ggatcaactg tgagaagggt taatttgcca tcatgttcag ctgagagaat cgtaccctgg
    20641 ctgacatatt ttttcatcat tttacgtggt ttgaggttag caacgatttg aactttcttg
    20701 ccgaccaatt cttgttcatt tggatagtat tttgcaattc ctgaaagaat ctgacgatct
    20761 tctccatcac cagcatccaa gcggaattga agcaacttat ctgaaccttc tactttagac
    20821 acttctttga cttctgcgac acggatttca accttgtcaa agtcttcaaa cttgatttca
    20881 tccttgttta gtttgagctc aacttcgtcc ggattccatt ctttttcgac tgctggttta
    20941 ttgccttcca tttgttcctt gatataggcg atttcttctt ccatatttag acgtggaaag
    21001 ataggtgttc ctttggcaac tacagtcaca tctgctggga agtcagccaa actcaagttt
    21061 tcaagactag aaacttcttc caaaccaagt tgagtcaaaa ctgcacgact agtttccatc
    21121 ataaatggtt caatcaagtg agcaactaca cgaatgctgg ctgccaagtg gctcatgaca
    21181 cttgccaatt ggtcacgaag agcttcatcc ttgtccaaga cccatggtgc agtctcatcg
    21241 atgtatttat tggtacgaga gatcagagtc cagactgctt caagcgcacg tggatagtca
    21301 actgcttcca tgtgtgtatg gaagtctgcg attgattgtt ctgcaacctc agcaagaaca
    21361 tgatcaaatt cagtcacacc ttctacatag gcagggattt gtccatcaaa gtacttatta
    21421 atcatggaaa ccgtacggtt aaggaggttc ccaaggtcat tagccaattc atagttgata
    21481 cggccgacat agtcttcagg agtaaaggtt ccgtctgaac caactggaag gttacgcatg
    21541 aggtagtaac gaagtggatc tagtccataa cgctctacca acatttcagg gtaaacgaca
    21601 ttcccttttg acttagacat ttttccgtct ttcatgacaa accaaccatg ggcaatcaaa
    21661 cgatcaggta atttaacatc caacatcata agaaggattg gccagtagat agagtggaag
    21721 cgaaggatat cttttcctac catatggaag actgttccat tccagaactt gtcaaagtta
    21781 ccatgttcgt cttgagcgta gccaagagct gtcgcatagt taagaagggc atcaatccaa
    21841 acgtagacaa cgtgttttgg atttgatggg acaggcactc cccatgtaaa ggttgtacga
    21901 gataccgcca aatcttccaa acctggctcg atgaagttgc gtagcatttc attaaggcga
    21961 ccatctggcg tgataaattc aggatgagct ttgaaaaatt cgaccaaacg gtcttggtat
    22021 ttgctaaggc gaaggaagta tgattcttca gaaacccatt caacctcatg acctgatgga
    22081 gcaataccac cagtcacatt tccagcttca tcacggaaaa cttctgccag ctggctttct
    22141 gtaaagaatt cttcgtctga tactgaatac caaccagagt attcacccaa gtagatatca
    22201 tcttgagcaa gtaagcgttc aaagacctgt gcgacaactt tttcatggta gtcatcagtt
    22261 gtacggataa atttatcgta tgagatatct agtaattgcc agagttcttt aactccaacc
    22321 gccattccat caacataggc ttgaggtgta ataccagctt cttccgcttt ctgctggatt
    22381 ttctgaccat gttcatcaag acctgtcaga taaaatacat cgtagcccat caggcgtttg
    22441 taacgtgcta ggacatcaca tgcgatagtt gtgtaggcag aaccgatatg aagtttccca
    22501 gatggatagt aaatcggcgt tgtaatataa aaattttttt cagacataat ttttcctttc
    22561 caggcaaatg aaacctgttt ttctaacact tcattatatc acatttttaa tgaatttcga
    22621 tagggaaatc catacaaaaa caagatagac gagtgtccat cttgttgatc tcattcataa
    22681 cgaagggctt caattggatc aagtttcgat gccttgttgg ctggcaagac tccaaaaatc
    22741 ataccaacac tagccgaaac tgcaagacta aatagggcaa ctgggattga tactccaact
    22801 tctatacctt ctattaaacc ttgcagtaac aaacctgcta aggcagttaa accacttgca
    22861 attgtcaagc caattaagcc acctaacaag gtcaaaatca tggattcaat caaaaactga
    22921 attaaaatat tggcacgtgt tgcacccaaa gccttacgaa gaccaatctc acgagtgcgc
    22981 tctgtcaccg aaaccagcat gatgttcatg acaccagttc ctccaacaaa gagagaaatc
    23041 cctgcgatgg aactaataat cgtcgtcata aaactaaacg attgttgaat ttctgcaaat
    23101 acaagggact catctgccac ctggtattct ccctgttgta agcctgcaag ctctgtcatt
    23161 tttcgtgcca gttctggacc cagagttggg gttaaactgg tatcattcac tcgaaagaca
    23221 atattagcta tttcatctac attaaaattc gcagcaaggg agatattggt agtaataggc
    23281 aagccaccaa acccatatat ttttgatctt ttagcctccg gactagtata aaccccaatg
    23341 acccggtaac taaatccatt gacttctaca accttgttaa tagcctcttg aggagattca
    23401 aataaactaa tggacaattc ctcatctagc aaaatgacac ttgcaaactc tttgaaatct
    23461 tgctctctca gactacgacc tgcaataatt tcattcttaa cagcgtccat gtaagttctg
    23521 tttccacctg tcaaattagc attctcaacc tttttatctt gataggtcaa gatggcattc
    23581 gttgaattgg ttacatagta actatccact cccttcagtt tagctgcctc ttggacccag
    23641 gattcttgcg gttttggcgg ttcaacagga acttcctctt cctttccaga aaccgtaaaa
    23701 gctgattgtt tctgagtaaa agacccgtct ttactttttt taggagagaa aaagacgcta
    23761 atatttttct gagatttagt catatcttta ttgacttgac gagataggga atcacccaaa
    23821 gccataatca caacaactga tgaaacaccg ataataatcc caatcatagt aagcaaagaa
    23881 cgcatcttgt gagccatgat agatgaaaag gcaaatttca gattctgcat cttagttttc
    23941 ctcctttcct aactgagcac tgtcagacga aatgacccca tcccgaatga caatctgacg
    24001 tttggcatag gcagcaatct caggctcatg cgttaccatg ataatggttt ttccttcttt
    24061 attcaaatca accaataatt gcataatttg gttacctgtt ttggtatcca aggctcctgt
    24121 cggttcatcc gctaggataa tagaaggatt gtttaccaag gcacgcgcaa tggctacacg
    24181 ttgcttttga ccaccagata attctgaagg taaatggtga ctacgttctg tcaattcaac
    24241 cttgtctaaa tattcctcag ccaacttgcg acgttttgaa gacgaaactc ctgcgtaaat
    24301 caagggcaat tctacatttt gcagagcatt gagcttcgat agaagaaaga actgctgaaa
    24361 gacaaaaccg atttgttggt tacggacctt agctagttgt ttttcaccaa gcccagccac
    24421 ttcttgacct tcaagataat attctccact ggttggtgta tccaacatgc caatcgtatt
    24481 catcagagtg gacttaccag acccagatgg tcccatgatg gctacaaatt caccctcatt
    24541 cacttctaga ttgatatttt tgagaacctg cagttcttgg tcaccattac ggtaacttct
    24601 gaagatattt tttagactaa ttagttgctt catcagcctt cacctctttt ccttcttcca
    24661 aggaagatgt tggattactg atgaccttag caccgttcgt taaaccagaa gtgatttctt
    24721 gattttctgc gtcagcattt cccaatgaaa cctcaacttt tttagccttt tgttgttcat
    24781 ccacaatcca gacataattt ttactatcat ccattactag actgctaaca ggaacaagaa
    24841 tagccttagt tttgctttta acctcaatgt tgacagaaaa accttgtttc aaatcaccaa
    24901 cctcgcctgt cacatcaata gtataagggt atttagaacc tgtattattc ccggctgctg
    24961 gactagctgc ttcaccattg tttttaggat agtcagaaat atagcttaat ttcccagtcc
    25021 attttttatc aggatacact ttagaagtaa agcttacttc ttgacctaca gaaaggttgg
    25081 ctagattgta ctcagacaat tctcccttga cttgtaaatt ttcattgctg acaatatgaa
    25141 ccataacttg gctcgcccct gttggagatt tagaaacatt gctattgact tcgactacag
    25201 ttccctctag ggtactgaga acagttgttg catccaattg actttgagcc ttgcttaatt
    25261 gcgccgcagc atctgcacgc gcatcacggg catcacccaa ttgagcgtca atagaagcaa
    25321 cagaatttcc agccactgga gttgggcttt gcaccgttgc atcttctcct cctactggcg
    25381 ctggtaactg tggagccgga gctgaagcgg cttcatttcg tgcttgattg agttcattga
    25441 tatgacgatc tgccctagct actgctcgac tagctgaatc ataggccgcc tgcgcttctg
    25501 aactactgta cttgactaaa gcctgccctt cgctgacctt atcgcccaca gaaacaagga
    25561 tttcatctaa atcaccctta ctagcatcaa aataaacata ttgttcattt tttgctgtta
    25621 ctgtccctga caataaaaca gaggaggcca cgcttccttc cttggcaaca acaagatgaa
    25681 taggctcatc ttttagagca gtctgagaag gttgtctaaa gagtaaaatc cccccagcac
    25741 ccaatacaac tacactcgca gcaccgattg ctgcatacag ttgccacttt ttagctttac
    25801 cattcttttt cttcataatg aaactccttt tcttttttac aatactttgc tattatacca
    25861 aatttccctc cagcaaacaa tacagttcag gattaaacaa tcgttcggaa ttttgctttt
    25921 ctattgtact agatatataa tacactatct tgacttattt ggcaagcttt tttgcaattt
    25981 tttgtgcgta gcagattttt gcaatcaaat caaaaaaaga ataaaatata actatcaaaa
    26041 aagacgctct actatttaaa agattacaaa ggagttttca atgagagaat atgatatcat
    26101 tgctatcggt ggaggtagcg gaggaatcgc taccatgaac cgtgctggtg aacatggagc
    26161 caaagcagcc gttattgagg aaaagaaatt aggtggaacc tgtgtcaacg tcggttgtgt
    26221 tcctaaaaaa atcatgtggt acggggcaca aatcgctgag actttccatc aatttggaga
    26281 agactacggc tttaagacta ctgatcttaa ctttgacttt gcaaccctac gtcgcaatcg
    26341 tgaatcctac attgatcgcg ctcgttcttc ttatgatggc agttttaaac gcaacggtgt
    26401 agacttgatt gaaggtcatg ctgaatttgt agattctcat actgtaagcg taaatggtga
    26461 actgattcgt gctaaacata tcgtgattgc tacaggtgcc catccaagta ttcctaatat
    26521 tcctggtgct gagctaggtg gctcttctga tgatgtattt gcctgggaag aacttccaga
    26581 gtcagttgcc attctaggcg ctggttatat cgccgttgaa ttagctggcg tactccacac
    26641 ttttggtgtc aagacagatc tctttgttcg ccgcgatcgt cctttacgtg gttttgattc
    26701 ctacatcgtt gaaggtttgg tcaaggaaat ggaaagaaca aacttaccac ttcacactca
    26761 caaagtccct gtcaagttag aaaaaactac tgacggcatt accattcatt tcgaagatgg
    26821 tactagtcac acagctagcc aagttatctg ggctacaggc cgccgtccaa acgttaaggg
    26881 cttgcaactt gaaaaagctg gagtgactct gaatgaacgt ggctttatcc aagtagatga
    26941 ataccaaaat accgtcgttg agggaatcta tgctctaggt gatgtaacgg gcgagaaaga
    27001 actgactcca gttgcaatca aggccggacg taccctatct gaacgtctct ttaacggaaa
    27061 aactactgca aaaatggatt actcaactat tccaactgtt gtcttttcac accctgctat
    27121 cggaactgtt ggtttgacag aagagcaagc tattaaagaa tacggtcaag accaaatcaa
    27181 ggtttataaa tcaagctttg catctatgta ctctgcttgc actcgcaacc gtcaagaatc
    27241 ccgtttcaaa ctcataacag ctgcttcaga agaaaaagtt gtcggacttc acggaattgg
    27301 ctacggcgtt gatgaaatga ttcaaggatt tgctgttgct atcaaaatgg gagcaaccaa
    27361 ggctgacttt gacgcaactg tagcgattca cccaacttca tctgaagaat ttgtaaccat
    27421 gcgttaatcg aaacaaaaga agctgatata aatatgtctg cttctttttt aatgattttc
    27481 gaatcaacaa aaaaattatc caacttgact agtcacgaaa atcggctgtt acagtttttg
    27541 ttaccttgtt tttaaaaaat ggttgacatc gactcctata tgagtataat agttactata
    27601 cattagaaaa gaggttaact attttgaaaa aagctcacgt ttatgctatc cctgctattg
    27661 gggctgctct cattgctgct ttggcacaaa tcagtcttcc aattggacct gttcccttca
    27721 ctctgcaaaa ctttgcaatc ggcttgattg ctactgtctt tagaccgaga gaggctgtac
    27781 tttctgctgg actctatctt cttctaggtg ctatcggtct tcctgtcttt gcaggaggtg
    27841 gagctggttt tcaggcttta gttggcccta ctgcaggcta tctttggttt tatctcgttt
    27901 actctggact tacttcctct ctaaccaaca gcaagagtgg tgttgttaag atttttcttg
    27961 caaacctctt gggtgatgcc cttgtctttg tcggcgggat tctcagcttg catttcctag
    28021 ctggaatggc atttgaaaaa gctcttgttg tgggggttct tccctttatc attccagacc
    28081 ttggcaaact tctggctatt agttttatta gccgtcccct acttcaacgc cttaaaaatc
    28141 aggcttactt tactaactaa aaaaggatac cgagttatca tgactcaata tccttttctt
    28201 ttattttgaa aacttatact caatgaaaat caaagagcaa actaggaagc tagccgcagg
    28261 ctgctcaaaa cactgttttg aggttgtgga tgaaactgac gaagtcagct caaaacattg
    28321 ttttgaggtt gtggatgaaa ctgacgaagt cagtaaccat acatacggca aggcaaagct
    28381 gacgcggttt gaagagattt tcgaagagta ttagttgcct ttaaaggcat ccaccatcgt
    28441 ttgaaattct tcatttgaga gagtaatccc tttgcccatt ttagtatggt ctggactcca
    28501 agcacgaata tcaaactttg caggggcacc attaaagctc acacggttaa tttccttggt
    28561 ccaacctttt tcgttttcag aaagagtcaa caagtgctct tcgatttcaa atgtaaattc
    28621 tgccattttc ttctcctttt ttagtttcat tagtttattc gtaaaatctt gtagatttta
    28681 ggaaaatttt atataatatt gatataaaag aagggaggcc aatatgagac ataaattcca
    28741 gcaagttcta aataaaatac atgatttttt aaatggatat gaccaacctg accagactga
    28801 aaccaactcc cttacagcca ctattgaaga ggctatccag aaacaaaccg ctgttcacct
    28861 tatcttatct gagacaagct ttacaggtga catcatcaaa tatgatcagc aaggccagca
    28921 aattatcgtg aaaaattttt ccaaaaatgt gagccggatt atccgtataa gcgatattca
    28981 acgcctgcga tttgtcccct caactgtcca aacagcccaa aaaaatagat ttaagaaaga
    29041 gtgagatgta gttgcttcat cccactcttt tttcttagcg aatttgttca aaatgtaaat
    29101 gaactgcgat atgatctcca taaccacttc tttccaagtc acgttgtaaa cgataggaaa
    29161 tgtagtgttc tgcaatggta atgtaacctg cgcccaataa acgatgttca accatagatt
    29221 gaatcatact gatagtcgca cgttccacct tggcttcttg taaatccaaa actaccttct
    29281 tagtgacttg agcaagattt tgacgcaaat catctgtcaa aacataaaca gtttgggctg
    29341 ccttcaagat ggcttggtaa atcttatctg gattaaattc agcaatttcg ccattacgtt
    29401 tgattacttg cataggtttc tcctttattc tttgttttct ttgatttctg ccagcatttt
    29461 ttcttcttct actgtcagtt gataatgttc aagtaaatcc ggtctgcgct cgtaggtttt
    29521 ctttaaactc tcgtacaatc gccactgacg aatcttttca tggtggccac tcatcaatac
    29581 atctggcacg accatgcctc gataatcata aggacgtgtg tactgaggat attctaaaag
    29641 acctgaagaa aaactatcat cttggtggct agactccttg ccaatcactt ctggaatcag
    29701 gcgaactgta gcatcaatca tggtcatagc tgccaattct ccaccagtga ggacatagtc
    29761 gcctagggaa atctcatctg ttaccaaggt cttaatgcgc tcatcataac cctcatagtg
    29821 cccacagata aagattagct cttcctcttg agccaaatct tcagcataag cctgatcaaa
    29881 ctgctttcca gcaggatcga ggagaataac gcgcggattt ttcttttcaa tagcatcaaa
    29941 ggaatcgaaa ataggttgta ctctgagcaa catgccctga ccgcctccgt agggctcatc
    30001 atctacatga cgggcctttt cagcattttc tcgaaaatta tgatactgga tatccaagag
    30061 ccctttttct cgagcctttc caacgattga gtgctccagt ggagaaaaca tctctggaaa
    30121 gagggttaaa atatcaatct tcatcgtcta acccttctaa gatttccaca tcgacccgtt
    30181 tatttggaat atcaacattg agaaccactg gtgggatata aggtaaaagc aaatcacgtt
    30241 tgccttttcg tttgaccacc cagacatcat tagcacctgg ttgcaggatt tccttgatgg
    30301 ttccaaccaa gctatcaccc tcatagactt ccaaaccgat aatctcgtga tagtaaaatt
    30361 caccatcgtc taggtcattc aaatcttcct cagcgacctt gagactgtat cccttgtact
    30421 tttcgatagt attgatatgg tacatatctt tgaatttaat aatgtcaaag ttcttctgtt
    30481 tacggtggct agcgatggtc actgtttgga caaactgatc tttttcatca aacaaagcca
    30541 gctcagctcc ttttttaaac cgttcttctg caaaatccgt cacagacaag actcgcatct
    30601 caccctgtaa tccctgcgta ttaacgattt tcccaacatt aaagtagttc atcttgtctc
    30661 ctgtaatctc cttttttcca tattattcta acaattctcg aataatagcc gcaatttttt
    30721 ccgattctga ccattgtaaa taatggtgat tccctcctaa aatgagttta gtattggaag
    30781 tccaatattc tgattctctg tactcttttt ctctataagg ctgacaaaaa acaaatacag
    30841 gaatatgagc ttctatagat acatcctcaa aatcttcctc agtaatctct ccagatatct
    30901 gaaattctgg atcttgattt tccaactcta agcctttttc ttgcattaat tcccagattt
    30961 ttttattcgt ttcaggacta aatgttgctt gagttaagtt cttaaaataa agttcaggac
    31021 cacactcgtc aatcagcctc atctgctctt ccatttctgg ataaggattt tctgaaaaat
    31081 cagcaaacat gactttttta gttgtcggtt caattgctac taaagtctga cgcttaattg
    31141 gtttctcgag taatttgcaa gctaaaattc cactccaact atgtgcacaa agtatatatt
    31201 cagaaattcc taattcttca agtacttcat aaaccgcatc tgcaagatta tctagatttt
    31261 ttccagcttg gtcatgaatc ggactcctac ctgtgttcgg aaaatcaatt gtcaaataac
    31321 caattgtagg aggaggtttt tcaagtataa gtgaaaaatt ttcataactt ggtagcaaac
    31381 ctgctccgtt tagacaaact agcactttct tttgcttttg ataagtaaca gagaggctac
    31441 caatttctgt agatacttca aacctcttca taaagaaatc cactgattct atataatgaa
    31501 ttattaaaaa tccttatcct ttattttatc acgttccaag gattttctca agttggagga
    31561 aggggacaat atctctactt tcccttcaat aatccttcca aattatgttt atgttggtaa
    31621 ttaacggctg cggttttgtc tttctcaaag acagtcttgg taaggtcaat atgattaata
    31681 gctacgattg cgacggtgta gtaaatgata tcagccagtt ctctggcaag ttcctcgttc
    31741 gaatcctatc ccttcttttc gaccagagcg cctattcaaa acctcggcta cttctccgac
    31801 ttcctccact aacttcataa aaagaccttc atcagtccga gactgctgtt aatgttcgat
    31861 taagtagtct tggaattgcc taaacgttca atcttttata gtatattgaa actagaatag
    31921 tacaccttt
//
LOCUS       DAVKGC010000010        28985 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKGC010000010 DAVKGC010000000
VERSION     DAVKGC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28985
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.010"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            69..980
                     /locus_tag="VW049_000395"
     CDS             69..980
                     /locus_tag="VW049_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9431576.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            1210..1908
                     /gene="dapD"
                     /locus_tag="VW049_000396"
     CDS             1210..1908
                     /gene="dapD"
                     /locus_tag="VW049_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9431577.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            1976..3106
                     /locus_tag="VW049_000397"
     CDS             1976..3106
                     /locus_tag="VW049_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9431578.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            3118..3657
                     /locus_tag="VW049_000398"
     CDS             3118..3657
                     /locus_tag="VW049_000398"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9431579.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            3641..4318
                     /locus_tag="VW049_000399"
     CDS             3641..4318
                     /locus_tag="VW049_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9431580.1"
                     /translation="MKEIFDRRYSVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(4390..5289)
                     /gene="galU"
                     /locus_tag="VW049_000400"
     CDS             complement(4390..5289)
                     /gene="galU"
                     /locus_tag="VW049_000400"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9431581.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(5311..6327)
                     /locus_tag="VW049_000401"
     CDS             complement(5311..6327)
                     /locus_tag="VW049_000401"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9431582.1"
                     /translation="MKKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSIILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHETNIKDTIYDIMNNEFKAE
                     NEWS"
     gene            6539..6920
                     /locus_tag="VW049_000402"
                     /pseudo
     CDS             6539..6920
                     /locus_tag="VW049_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(7094..7519)
                     /locus_tag="VW049_000403"
     CDS             complement(7094..7519)
                     /locus_tag="VW049_000403"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9431583.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGETCLTE"
     gene            complement(7587..8237)
                     /gene="phoU"
                     /locus_tag="VW049_000404"
     CDS             complement(7587..8237)
                     /gene="phoU"
                     /locus_tag="VW049_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9431584.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(8252..9004)
                     /gene="pstB"
                     /locus_tag="VW049_000405"
     CDS             complement(8252..9004)
                     /gene="pstB"
                     /locus_tag="VW049_000405"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9431585.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(9006..9821)
                     /gene="pstA"
                     /locus_tag="VW049_000406"
     CDS             complement(9006..9821)
                     /gene="pstA"
                     /locus_tag="VW049_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9431586.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(9814..10677)
                     /gene="pstC"
                     /locus_tag="VW049_000407"
     CDS             complement(9814..10677)
                     /gene="pstC"
                     /locus_tag="VW049_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9431587.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(10795..11670)
                     /locus_tag="VW049_000408"
     CDS             complement(10795..11670)
                     /locus_tag="VW049_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU9431588.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(11837..13168)
                     /locus_tag="VW049_000409"
     CDS             complement(11837..13168)
                     /locus_tag="VW049_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9431589.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(13169..13876)
                     /locus_tag="VW049_000410"
     CDS             complement(13169..13876)
                     /locus_tag="VW049_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9431590.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGIEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(13960..14445)
                     /locus_tag="VW049_000411"
     CDS             complement(13960..14445)
                     /locus_tag="VW049_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9431591.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            14431..14988
                     /locus_tag="VW049_000412"
                     /pseudo
     CDS             14431..14988
                     /locus_tag="VW049_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15303..16994)
                     /locus_tag="VW049_000413"
     CDS             complement(15303..16994)
                     /locus_tag="VW049_000413"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9431592.1"
                     /translation="MNIKELIASELSSIIDSLDQEAVLKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            17130..17576
                     /gene="argR"
                     /locus_tag="VW049_000414"
     CDS             17130..17576
                     /gene="argR"
                     /locus_tag="VW049_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9431593.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            17627..20161
                     /gene="mutS"
                     /locus_tag="VW049_000415"
     CDS             17627..20161
                     /gene="mutS"
                     /locus_tag="VW049_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9431594.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDCEGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            20308..22002
                     /gene="patA"
                     /locus_tag="VW049_000416"
     CDS             20308..22002
                     /gene="patA"
                     /locus_tag="VW049_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9431595.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELFGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(22332..22595)
                     /locus_tag="VW049_000417"
                     /pseudo
     CDS             complement(22332..22595)
                     /locus_tag="VW049_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            22793..24559
                     /gene="patB"
                     /locus_tag="VW049_000418"
     CDS             22793..24559
                     /gene="patB"
                     /locus_tag="VW049_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9431596.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMIDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            24698..25387
                     /locus_tag="VW049_000419"
     CDS             24698..25387
                     /locus_tag="VW049_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9431597.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            25391..25570
                     /locus_tag="VW049_000420"
     CDS             25391..25570
                     /locus_tag="VW049_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431598.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            25557..26906
                     /locus_tag="VW049_000421"
     CDS             25557..26906
                     /locus_tag="VW049_000421"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9431599.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVNFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            27030..28490
                     /locus_tag="VW049_000422"
     CDS             27030..28490
                     /locus_tag="VW049_000422"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9431600.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELVAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            28504..28938
                     /locus_tag="VW049_000423"
     CDS             28504..28938
                     /locus_tag="VW049_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9431601.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         8419 a         5691 c         6314 g         8561 t
ORIGIN      
        1 ataattctct aaatgaagtg gccaatcaat ggattgccac ttttttcctt tcgtgttaca
       61 ataagggtat gaatcagtat cagaaaaaga ttgttaacgg aaaaatttat tcgctcctat
      121 ccggcttaat atggggaatc tgtggaattt taggagagta cttctttact cattatcagg
      181 tgtcttcggg ctggattacc tctatgcgtt tgacactggc agggagtctt gtactcattt
      241 ggtctgcaat acaattaaaa tcgcaagtgc tagatatttg gcgagacaag aaaaattacc
      301 tgcccttttt agcttatgct attttgggga ttttttcagt ccagtatttt ttctatctct
      361 gtgtagaata ctcaaatgct acgacagcaa ctattttaca gtttattagc cctgtcttta
      421 tcctctttta caatcgcttg gtttatcaaa aacgagcgtc aaaaagcgct gttttctatg
      481 ttttggttgc catgctgggt gtttgcttga tggcgacaaa gggagatctc tctcagttat
      541 ccatgacgcc gctagctctt ataacaggtt tgctgagtgc catgggtgtt atgtttaatg
      601 ttatcttgcc ccaacctttt gctaagcgtt atggttttgt tcctacggtt gggtggggga
      661 tgattttggc aggtttgttt agcaatgtcc tctcgccggt ttatcagctt tcctttactc
      721 ttgatatttg gagtatcttg atttgcctca ttatcgcttt ctttggaacg gcttttgctt
      781 ttttcatttc catgaaggct gtgtccttgg tttctccttt ggtggttgcc gttatcagtg
      841 ccagtgaacc tctctcttct gctctcttga gtgttttgtt cttaggatta gtagtggatt
      901 ggtccctcct tctagctata gccttgatta ttttacccat gatatttttg tctatagaag
      961 aagcgaaaga aagtagataa aaagtctttt cttaattcta aaagtgtgct atactagaat
     1021 agtcaataaa acacggggag atagttgtga agagtgtttt taggttgtat cggtaggggc
     1081 ttgcttttac cgacagcacg ttttatctca catccctaaa aaatcatacc taaaaacaga
     1141 caaaaaggtt tcaaatttga ggcctttttt ggtagaatag gtatcattat aaagaatgag
     1201 aggcctttta tgactgctac aaaaatgaat gctcaagaaa ttatccaatt tatcgccaat
     1261 gctgaaaaga aaaccagtgt caaagtaacc tttgaggggc aactcgcaac tgctgtgcct
     1321 agctctgttg tcaaactagg aaatgtccta ttcggagact ggaaggacgt ggctccgctt
     1381 cttgaaggtt tggtagaaaa tcaagattat gttgtcgagc aagatgctcg taattctgca
     1441 gttcctttgc tagataagcg tgctatcaac gctcgtatcg agccaggtgc tattatccgt
     1501 gaccaggtgg aaattggtga caatgctgtt atcatgatgg gagctgttat caatatcggt
     1561 gctgaaatcg gtgctggaac catgattgac atgggtgcca tccttggtgg ccgtgctatc
     1621 gttggaaaaa atagccacgt tggtgcaggt gcagttttgg caggtgtgat tgagccagct
     1681 agtgctgaac cagtccgtgt cggagacaat gttcttatcg gtgctaatgc agtggttatc
     1741 gaaggagtcc aaatcggtag tggttcagtt gtcgctgcag gagctatcgt tacccaagat
     1801 gtcccagaaa acgtggtagt agcaggtgtt ccagctcgta tcatcaaaga aattgatgcc
     1861 caaacccaac aaaaaacagc gctagaggat gcgcttcgta ccttgtaatt gtaaaagtaa
     1921 aaaagaggcg gaaccctttt tccagcctct ttctgctata tcggaggaca gatagatgtt
     1981 agatttgatt cagactagac gagctttaca ccagattcca gagattggct tggaggagtt
     2041 caagactcag gcttatttgc tggatgtgat tgagaaattg actacgggca aggattttgt
     2101 tcaaattcgt acttggcgga cagggatttt ggtctacctg cagggaagtc agccggagcg
     2161 aaccattggt tggcggacag atattgatgg cctgcctatc gtcgaacaaa caggtctacc
     2221 ttttgcttct caacaccaag gtcgcatgca tgcctgtggc catgattttc atatgaccat
     2281 tgccttaggc tgtcttgagc gcgcccttga ggagcaaccc aagaataatc tgctctttct
     2341 atttcagcct gctgaagaaa atgaagctgg tggtatgctc atgtatgagg atggtgcttt
     2401 tggagattgg ttgccaaacc aattctatgg tctccatgtt cgtccagatt tgaaagtcgg
     2461 ccagattgcg actaatactc atacactctt tgcaggaact tgcgaggtga agatccgttt
     2521 caaaggcaaa ggtggccacg cagcttttcc tcatgaagcc aatgacgcct tggtggcggc
     2581 tagttacttt gtgacccaag tgcagtcagt tgttagccgc aatgtcaacc caatcgaggg
     2641 agcagtggtg accttcggcg tttttcaagc tggaaccacc aacaatgtca tcacagacac
     2701 agctttttta catggaacta ttcgtgcctt gacacaggac atgagtctct tggtgcaaaa
     2761 gagggtaaaa acagttgcag aaggggttgc agctgccttt gatatggaag tcgaagtgga
     2821 actcaaacaa ggtggatacc tacctgttga gaacaatcca gccttggcgc gtgaactgat
     2881 ggacttcttt gacgaaaaag acggaatcga gttgattgat atcgaacctg ctatgacagg
     2941 cgaggacttt ggttatctcc tttcaaaggt tgatggcgtg atgttttggc taggtatcga
     3001 tagtccctac gctcttcatc accctcaaat gagtcctaag gaagaagtct tagctattgg
     3061 ggtggctgcg gtgtctagtt tcctgaaaaa gaaggcagca gaatagaggg cttgtatatg
     3121 aaatcggaat tacgcaagca agtcttgcat gaaatgaagg ctttatctca agaacaaaaa
     3181 caggctatag accaagcttt aaccgagcga attttacaac acccctttta tcaagaagcc
     3241 aaggtcatcg caacctacct ctctttttct catgagtttc aaacgcggga actgattgag
     3301 caggcgctga aggacggcaa gaaggtttta atacccaaaa cttatcccaa ggggcgcatg
     3361 gactttgtag tctatgatcc gcagcagttg gtaaaaactg cctttgcctt actggagcca
     3421 cagggagatt tggaagtggt ggatgtatct cagattgatt tgattcatgt tcctggcctg
     3481 gcttttacga cggatggata tcggattgga tatggtggag gttattatga ccgctatctg
     3541 gaacattttt ctggtcatac tttgagtacg gttcatcctt gtcaaattca ggactttata
     3601 cctgaaaacc atgatattcc tgttcaggag gtattaattg atgaaggaaa tctttgatag
     3661 acgttactct gtgacgagtt tcttcctctt agtgacggcc ttggtatttt tactaatgtt
     3721 ggtcactgca ggcggaaact ttgacagggc agatacatta tttcgatttg gagccatgta
     3781 tgggccagct attcgcctct ttcccgagca ggtttggcgt ctcttgtctg ccatttttgt
     3841 tcatattggg tgggaacatt tcattgttaa tatgctttca ctttattatc ttggaaggca
     3901 ggtagaggag attttcggct ctaagcagtt cttttttctc tatctcttat caggaatgat
     3961 gggcaatctc tttgtttttg tatttagtcc taaatcctta gcagcaggcg cctctacctc
     4021 tctttatggg ctatttgccg cgattattgt tcttcgctat gcaactcgca atccttatat
     4081 ccaacagcta gggcaatcct atctgacact ttttgtggtt aacattattg gaagtgttct
     4141 gattccagga atcagcctag caggccatat cggtggtgca gttggtggcg catttctagc
     4201 agttatcttt ccagttagag gagaaaaacg gatgtataac accagccaga gattaggagc
     4261 ggtagtcttg ttcgtaggac tcgccatttt gcttttctac aagggaatgg gaatgtgacg
     4321 aagttgaaag aaaatttgtg taatgaaaaa agataaggca ttttttgaac cttatctttt
     4381 tattttattt tattccttct cagtcaattc ttttccaagt tggatgaggt aattcttcaa
     4441 atcatctttg acttgtgggt gtttgagggc gtagtcgatg gatgttttca tgaagccaaa
     4501 cttgtctccg acatcgtaac gagccccatt gaactcacga gcaaatacac gttgtgtttt
     4561 attgagggtg tcgattgcat ctgtcagctg aatttcattt cctgcacctg gagcttggtt
     4621 ttcgaggatt tggaaaattt caggcgtgag gaggtagcgt ccgataatag caaggtcgct
     4681 aggagcgtcc tctggagctg gtttttcaac aaaggtttca acactgtaaa gaccatcttt
     4741 tccttcgcct tgcggagcaa taaccccgta agcagatact tcgtcatgag ggactggcat
     4801 gacagcgata gtagacgcgt gggtacgctc gtagtcatcc atgagttgtt tggtaagtgg
     4861 aacagccttt tcgtctgtga tatccatcaa gtcatcacca agcataacga caaaaggttc
     4921 atttccgacg aaagccttag cttgcaaaac agcatctccg agaccgcgtg gatgagtttg
     4981 gcggataaaa tgcagacgca tgtcagttgt tttatcaact agcttcaaaa gatctgtttt
     5041 ccctttttct ttgaggttat attccaattc gaagtttgaa tcgaagtggt cctcaataga
     5101 acgttttgat ttaccagtga caactagaat atcttcaata cctgatttga gagcctcttc
     5161 cacgataaac tggatagttg gtttgtctac gattggcaac atttctttgg caagggcctt
     5221 ggttgctggt aaaaatcgag ttcctagtcc agcagcaggg atgactgcct ttctaacttt
     5281 tgatgtcata agaatccttt ctatagaggg ttaagaccac tcattttctg ctttaaattc
     5341 attgttcatg atgtcataaa tggtatcttt gatattggtt tcgtgataaa taacttggta
     5401 aatggcctgt gtaatgggca tatagactcc aagttcttgg gctagttcat aggctgctcg
     5461 agtcgttgaa attccttcga ttaccatgcc catattagct tctatatcag ctagggattc
     5521 tcctcgtccg agagcatctc cagctctcca gttacgagag tggatggaag ttcccgttac
     5581 gatcaaatct cccacaccag ataagccgct ataggtcaat ggactggccc cgagagccac
     5641 ccctaggcga gtaatttcag ccaagcctcg agcgatgatg gctgccttag cattatcacc
     5701 aaatccaaga ccatgtaaag ctccagcacc gacagcaata atatttttaa gagcaccagc
     5761 agtttcaacc ccgataacat ccgtattggt ataaagtcgg aagtagtgat tactaaatag
     5821 ctcctgaacg tattgagctg tttgtaaatc tttagaagca gcagttatta aagttaggtc
     5881 acgcacaatg gtctcttctg catgactagg tcctgaaaca acgacgatat cactacggag
     5941 atgttcagga atttcttctt caagaatgat tgataatcgt ttatggctat caggttctaa
     6001 tccctttgat gcgtgcatga tgataacctt atggtccaag gtttgtgcaa cttgctgggc
     6061 aacaagtcgt gtcacttttg ttgggacaac aaacaaaatt gcatccacat ctttcaatgt
     6121 ttctgctaag tcggtgtagg caatgatatt ttcgtctaga acgacatctt taaagtagtg
     6181 cttattagta tggtgtgtat taatttcatt gatttgctcg ggaagatttc cccaaatacg
     6241 tacctcgtgt ccattgtcat ttaagacttg tgaaagggcg gttccccaag aaccaggccc
     6301 caagacggcg acagtttgtt ttttcatgat ttcctccttg atagggttct ttctcttatt
     6361 ctatcatatt ctaggggatt ttgcacttgc attactgggg tatggtggaa aaatacacaa
     6421 aaaccgagac gaagggataa actcttagtc tttagttaaa atgagaagaa atctgataga
     6481 tagtttgatt cagtatatgc ttataataga ggtaaacaac tcaggaggtt cttgtaggtt
     6541 gcgagagttt ggcgaaaaaa ttaaaagatt acgtttggct aaaaaaatca gtcgttcaga
     6601 attttgtggt gatgagtctg aattaagtat ccgtcaatta attagaattg aaaatggaga
     6661 atccagacca acactaacaa agttaaaata tatcgctgaa cgtttggggg ttgaagatta
     6721 caagttgatg ccaagttata tagagttgga taaggaatac ctagaattga agtatttctt
     6781 gatgaggact cctacatacg aagatgaaac tatcgtccaa aagaaagaga gtgtttttgc
     6841 taagattttt gaagagtatt atgataggct acctgaggaa gaaagattta tcatcccaaa
     6901 ttattcatat ctggcactaa cgaactacac agttcaaaaa ttaccagaaa agctagttga
     6961 aatactgtcc ttctggtgat tttgcttaat ttctgatgtt cgagacatga ttgaggagat
     7021 ttgtcttgat tttttcctaa aaaagggcag actcctccct tggtttgatg ctcgattttt
     7081 aaaataaggt gacttattct gttaggcatg tttccccctg ccctctagct ctatatggaa
     7141 cagggagtat cagatttatc tggcagattg tatcaaataa ggcttgaaac tgttttgaat
     7201 aggcgtatag acttgagaat ttgagaatat gtcgtctagc agtagagaca tattttccgg
     7261 caatatgaag gaagagacgt cgaaaacgct tgatagtcaa ggcctttact tcatcaccag
     7321 ctagctgctt taaaaagagg tagagattat aagccagaca gcccatcatc atacgtactt
     7381 cattcttaat catggtcgaa ctatctgtct tatccccaaa gaatcctgct ttcctttctt
     7441 tgataaagtt ttcagcattc cctcgttcac gatagaattg aaacagctcc tcactatcta
     7501 cactgcttaa attgttcaaa tgaacttttt tacaaccata aaaaagagca cttaatcgta
     7561 ctcttctctt ttaaggatta gttgaattaa ttcaaatcca ctagttctcc tgtttctagg
     7621 tagactaggc gttcacaaat gttagcaatg taatcagcga agcgttccag atgccctatg
     7681 atataaaggt attgagttcc attgggaatt gaggtttctt ggtctttcat aagtccaatg
     7741 atttcctttg ataaggcata ataatattgg tcaatctgtt catctttttg agcaatacta
     7801 atagcttttg aggcttggtg caaaggaaag gcaaccaata aatcggctag catgctgagg
     7861 gataatttac ccatttggtg taactgttct tcgtcagggg ctagttgatt ttcttttagt
     7921 tgcaaaacag ctttggcaat gcctgccata tggtctccca tacgttcaag gtctgaacaa
     7981 gaagacatga tgctaatcac aaatcgaagg tcagacactt gtggctgctg caaggccaac
     8041 aaacgggcac aggtcaattc gatagcgctt tgaccttggt tgatagcatg atccttattg
     8101 ataattagct ctgccatctc cttgtctttg gaggctaagg ccagtaaggc ttttgaagct
     8161 gtttcaagga caagttgccc tagtcctaaa aaggattgtt ctaattcatg caattctaag
     8221 tcaaattgat ttctcatagg tttttccttc cttatccgaa ccgtcctgaa atatagtctt
     8281 ctgtgcgctg atcttttgga ttggtaaaca cgtcaacggt atctccaaat tcgcaaattt
     8341 ctcctgttaa gaaaaaagca gttttatctg aaatacgtga agcttgttgc atgttatggg
     8401 taacaatgat aatcgtataa tcctttttta gttgctgaat gaggtcttca atttttaaag
     8461 tggagatagg gtctaaggct gaagtcggct catccattaa cagaatatca ggttctactg
     8521 ctaaagctcg cgcaatgcaa aggcgttgct gctgaccgcc agataaggac atggcactct
     8581 ttttaagatc atctttgact tcttcccaaa tggctgcccc ttttaaagat ttctccacta
     8641 aggcatctaa ttgttttttg tctcgaatac catgtgtcct tggaccataa gccacgttat
     8701 catagataga catggcaaag ggattaggct gttgaaaaac catccctaca cgcttgcgta
     8761 gctgattaag gttgaatttg ctactataaa tatcttgctc atctaagagg acttggcctt
     8821 caatatggca agaaggaacc aaatcgttca tccgattaag ggtttttaga aaagttgatt
     8881 tgccacaacc agatgggcct atcaaggcag taatctgtct ttctggtaat tgaatcgaaa
     8941 tattttttaa ggcttgaaaa tccccgtaaa ataagtctag gtgtctaact gaaaatgttc
     9001 ccatactagg aagctccttt cacaagtttt cgagataata agcttgatag agtatttatc
     9061 attaaaacag taataatcaa aatcacaccg gtagcatagg cttcattgac atgtagcccc
     9121 tcacttgaca gcatatacat atgcagggct agagaacgac ctgaagacat gagactactt
     9181 ggcgtattgg tagaggtacc taatgtatac atgagggcag ctgtctcacc aacgatacgg
     9241 ccaatagcta gtatcactcc agctaaaata cctggcatgg caactggtag aacaattcta
     9301 aaaacagtcc gtaacttacc tgccccaagt ccataacttg cttgacgcat gctatcacta
     9361 acagataaaa gggcttcttc tgttgagcga ataatgactg gcaacaccat gataactgag
     9421 gttaagattc ctgataacag agagtattga aaacctaaga agactacaaa gaagagcatg
     9481 ccaaacagac caaaaacaat ggaaggaatc ccagacaagg tatctgaggc caatcgcatg
     9541 attttaacac aaagggaatc tttttttgta tattccacaa gataaaaacc agcaaaaatc
     9601 cctatgggca aggctaaaag aagagcacca aagaccagaa taacggtgga aataatcgct
     9661 ggcataaggg aaatgttctc agaagtataa gtccaagaaa agagggatag acttagatga
     9721 ggtaagcctt tgatgaggat aaaaccaatg attaaaaaga gagagccaaa ggttaaagct
     9781 gaaaaacaat aaacgagaag ttttagcagg tatttactca taagatgatt ttcctttcaa
     9841 gtaggcaaag taggcattaa tcaagagaat aaggaaaaag agaactgctg aggttgcaat
     9901 aagggcttcc ctatgctgac ctgatgcgta agccatttcc agaacaatat tggttgttaa
     9961 ggttctggtt cctgaaaaga gtccacttgg aataatcggc tggttgcctg ccaccaaaat
    10021 aactgccatg gtttcaccta ctgcgcgacc gattcctaaa ataactgctg ataaaatacc
    10081 agatctcgca gctggcaaga tgacactaaa aatactccgt tcatgactag ctcctagagc
    10141 caagctacca gaataatacg ttttgggaac tgttcggata gcagattctg acaaactgat
    10201 aatggttggc aaaatcatta ttcctaatag taacgaagcg gttaggacac tcatgccatt
    10261 tcctaaaaag cttctaatcc aaggcaccaa taattgtagg ccgaaaaaac cataaacaat
    10321 agatggaatg gccgccatca agttgatagc tgattttaag aagccataga cgggctttgg
    10381 acaataataa accataaaca ccgatgtcaa gatgcctgtt ggcaccccaa tcacaatcgc
    10441 tcctaaggta attaataagg aaccaacgat cattggtaaa ataccatagc ttgccggaat
    10501 gttcgttggc gaccaatcac tgcctaataa aaaacgggca aagccgtagt tagccatgaa
    10561 aggtaagcca ttactaaaaa taaagaaaca gattagcaaa atagctacaa cagctactgt
    10621 tgcactcatg aaaaaaattg ccctaaaaac tgcttctttg aaggcttgtt ttatcacatc
    10681 ttgtcctttc tagtgaagaa agtaagggag atacgacacc tccctacttg ccttctttat
    10741 cttattgtac gatgaaacgt ctgcatctct ttagagattt atggagcaaa cattttattt
    10801 aatcttgtcc caggtggtta atttgccact aaaaacgtct gcaagttcag ccatactgac
    10861 ttggcttgcc ttattgtcat tattgaccac aacagcaata ccgtctaaag caatagcatc
    10921 atgggtgaga ctcttacctt cttcaggagt taattcccta gaaaccatac caatatcagc
    10981 ggttttctcc ttaacagcgg taatacctgc tgaagaccca ttagaggtaa tatcaatcgt
    11041 aacttctgga ttttcttttt tataagcttc tgctaatttt tccattaaag aagatactga
    11101 agtggaacct acaacagaca acttgcctga taagtgttgg cttgtatatt ctgtggtttc
    11161 ggttttagct tcaataaatt tattatctgt gaccacttgt tgaccttgtt tggagtggat
    11221 aaagctgata aaatcttgac ctagcttgga aagattagaa gaccaaacaa tgttgaaggg
    11281 acgttgaaga gggtattcac catctaaaac tgtgtctcga ctagccttga caccatcaat
    11341 ctctaaagcc ttgacagatt tcgttaaaga tcccaaggag atgtagccga tagcattagc
    11401 attcccttga actgctgaga gaacaccttc tgtactattt tgaatcacag ctgttttggc
    11461 agtgttgtca atttttttat caccgtcttt tttgagaatc cctgtgattt ctgtgaaggc
    11521 accccgtgtt ccagagccat tttctcgtga aatcacctca atcgttcctg aagctgactg
    11581 tttggaagca gctgactgat tgccacaggc aacaagccca aatcctgata agccaatggc
    11641 tgcaagagta agcatttttt tgaatttcat aataatcacc tttatctcta tgtatttttc
    11701 ttgtgtaggc ttactacatt tatagtctaa caagtctttg taaaggttta tccctgattc
    11761 atgtaaagat tgtgtaaaga atcaaaaaaa gccacttttg aaaaatggct gctcctaaaa
    11821 atagctttaa aaattattag tcctgtgcga aagattggtt aggaagaaaa atcgtgaagc
    11881 aactgcctct gccaagctga ctcgtcaccg tgacttggcc acctaataat tgactgagtt
    11941 ctttgacaat ggcaaggcca agaccagtgc caccagtttg tctgcttcga cctttattaa
    12001 ctcggtaaaa acgttcaaaa atacgatcct gctctaattg actaatacca atccctgtat
    12061 ctgatacaga aatcttaatg ccttcgttca ccttttgggt cttgacctca attttccccc
    12121 cttgttcagt gtaacggatg gcattggata aaagattgag taagatttgg gaaagtaatt
    12181 gactatctga tacgagggtg acatcatctg gcacctgcac ctttagctgt aaatccttct
    12241 tcttgagctg aggttgcaag ctttgagtca aatcctgtac aaattctgcc aaagaaaggg
    12301 tcgtccattg tataggcatt tgttgagcct tagataaggt aagaagatgc tcaacaatat
    12361 gctcaagacg caaactttct ttgtaaataa tgtctagaaa gtcatccttg agcgcttctt
    12421 cttcagctga catcccctta atggtttcag caaagccctt aatcgaagta actggtgtcc
    12481 tcaattcatg ggaggcattt gagacaaagg ctaaatttaa cttttcataa gttctaatcg
    12541 ttgttaaatc atatagcaag acgagcacag cttccacaga ttgggtgggg ctaaaaacgg
    12601 gaactgctgt cacttctaaa atcaagtcac cctcatgaaa cccacttact tcttgtttta
    12661 accttgtttt ttgatcaaag gcttggtgaa ctaaattccg aatatccatc cgtttgaggt
    12721 catcaagtga acttatgtcg ccgtccacat cgggaaaata atgaggcaga gagcgactgg
    12781 ataataacat ctgaccttga gcggaaacta aaaacgtccc catggttagg tgcgacagaa
    12841 gaacctccat tgtttcggct agatccttgt attgctgatc ctgttgggag actttggttt
    12901 ttaggccaga cacatactga gccaaagact ttaagtcttc ttgccctttt tctaaaaagt
    12961 attcactact ggtcaagaga ggttggtgca aggtctcaaa agcaacttcc catttccaaa
    13021 ggcaaaagag ccagtagcca cctagtccca aagaaagggc tagaagaaag agaccgatgc
    13081 ctttactgat ccaagttaat gccatccctg caatcagaat gaggctaaca cttagattga
    13141 ctagccaaaa ttgaaggtag cgtttcatct ataactcctt gaacttataa ccataacccc
    13201 gaatggttcg aataaattga ggggctttag gattgtcttc aattttttcc ctcaacttac
    13261 caatatgaac gtccaccaaa cgtgtttcct gcccaaagtc atacccccag atacgttcca
    13321 aaagacgctc tctagtcagt gtcatgttgg gatgtttcat aagatagagc aagagttcaa
    13381 attcttttgg ggtcaaactc agtaacttat tcgccttgta gacttcatga cgctcagggt
    13441 atactttcaa ggtcccaaat agccaagaat cgtcagcgat attatctgaa tcatctcctt
    13501 cttgttctcc tttagttcgc ctgaggacag ccttgacacg cgccagcaat tctctagggc
    13561 taaaaggctt ggtcaggtag tcatcagccc ctaattccaa ggccaaaacc ttatcaaatt
    13621 catcactttt cgcagaaacc atcataattg gagttttgac gcctttggct ctcagccgct
    13681 tacaaacttc tatgccatct aattgtggta acatgatatc aagcaagata aaatcaaagg
    13741 gttctgtttc tgccaaagct aaggccttcc gtccatttgt caccaattga gtagaaaagc
    13801 cttccttact taaatggtag tcaagcaatt tcagaatgtg ttcttcatca tccactaata
    13861 agacttgttt tgtcatctat tatctcctat tggtaacatt ataacacaat tatcagaaat
    13921 cctaacattg ctaaatcaga ttaaatttgc ctatcaagac tagtatctgg tcaaacgctc
    13981 aatcatctcc ttgtgctctg gataggtcgc cagtagatct accctttcaa ataattcaaa
    14041 atcctcaaat tcaaaaccag gagcaacaag acaagaaacc agagcataat ccttatcaac
    14101 tgttgatccc caaatagtgc ccttaggaac acagtagtga agttgttgcc ctttggatat
    14161 gtccaggcct aaagtgactg cttcgtagtg accatctgct gtaatcatgt gaacagtaag
    14221 tggggatcct gcatgaaaat accagatttc atctgctgtc aatcggtgaa aatgtgaagg
    14281 attcgtttct tctaataaga aataaatact ggtataaagc gcccttccct taccagcaag
    14341 gtttatagtg tctgaagctt tttttgtttg tctaaaatag ccaccttcaa catggggagc
    14401 taactctaga gttcttatca agtcttcttt atccgtcgga gccaatgggt tgaagtaact
    14461 cttgttcaaa gtggttttac gatttcaaga actcctctca gttctgagga cacggtaatg
    14521 attgatgcga cggaagtaca aatcaatcgc cctaaaaaaa gaattagcga attattctgg
    14581 taaaaaaaat gccacgctat gaaggctcaa gcgattgtca caagtcaagg gagaattgtt
    14641 tctttggata tcgctgtgaa ctattgtcat gatatgaagt tgttcaaaat gagtcgcaga
    14701 aatatcggac aagctggtaa aatcttggct gacagtggtt atcaagggct catgaagata
    14761 tatcctcaag cacaaactcc acgtaaatcc agcaaactca agccactaac agttgaagat
    14821 aaagcctata accatgcgct atccaaggag agaagcaagg ttgagaacat ctttgccaaa
    14881 gtaaaaacgt ttaaaatgat ttcaacaacc tatcgaaatc atcgtaaaca cttcggatta
    14941 cgaatgaatt tgattgctgg cattatcaat catgaactag gattctagtt ttgcaggaag
    15001 tctattattt ggttaggtga attagtgaag cgtttaggca agtgtctctg gttacgacgt
    15061 catggactct aaatcgatta tatttagggg tcatgactag tgaagcagtt agctagttcg
    15121 catataagcg gctagcgtct aacaattagg aactttagtt ccaataactt taagattacg
    15181 acgttttagg acataaatcg atcatattta tgtcctaaaa ctagtgaagc gcctagccaa
    15241 agtccgaata ggatttggcg ttagttactt agattgcttt gcaatcaagt aactttggcg
    15301 atttacatct tctctggcgc ttctactcca agcaagcgaa gggcttcttt gagaacgact
    15361 gcggttgcgt agctgagggc tagacggctg tcgcgttctg ggctttcatc caagatacgt
    15421 gtatgtgcat agtatttgtt aaaggattga gccaggctaa ttgcaaattt agcaatgata
    15481 gaaggttcaa agttatctgc cgcacggttg ataatacgtg ggaagtcttg aatgagttta
    15541 atgatttccc agctttcagt atcattcaag ctatagttgc cagctgtttc tggtttgaaa
    15601 tcggctttgc gtaagataga ttggatacga gcgtaggcat attgaacgta aggtccagtt
    15661 tcaccctcga aggataccat agcctctagg tcgaagtcgt atccatttgt acggtcggtt
    15721 ttgaggtcat agaatttaat ggctccaacc ccaacagcat gtgctacttg gtctttgttt
    15781 tctagttcag gatttttagc ctcgatttgg accttggcac ggctaacagc ctctgcaaca
    15841 gtaggctcta gcaagatgac attcccttta cgagtagaga gcttcttccc ttcttttgta
    15901 accagaccaa aaggaacgtg ggtaatgtcg tcactccaat cgtagcccat ctcttgcaag
    15961 acagctttga gttgtttaaa gtgggcagat tgctcttgac caacgacgta gatagattta
    16021 gcaaattggt attcattttt acggtagagg gctgcagcca agtcacgtgt gatatagaga
    16081 gttgcaccat cagacttctt gatgagagct ggatgttcaa ttccatattt ctcaagattc
    16141 acaacttggg caccttctga ttcaagaagt agtccttttt cagaaagaat gtctacaact
    16201 gcatccatct tatcattgta gaaggcttct ccgttatagc tgtcaaattc aaccttcaat
    16261 tcattgtaaa ggcggttaaa ttccactaaa ctttcatcgc ggaaccattg ccaaagagcg
    16321 agagcttcct catctccatt ttcaagttta cggaaccatt cgcgcgcttc ttcatccaag
    16381 ctagggtcat tttcagcttc agcgttgatg cggacataga gtttaaggag ttcatcgatt
    16441 ggatgagctt ttacagcttc ttcgtcgccc cattttttat aggcaacaat caacatccca
    16501 aattgtttac cccagtctcc caaatggttg accttgaccg tttgataacc gattttttgg
    16561 aaaatatgtg acaagctatc tccgataaca gttgaacgca ggtggccaat agaaaatggt
    16621 ttagcgatat tcggactaga catgtcgata acaacatttt cttgtttacc aatattttgg
    16681 tcagcatagt gttctttttc agtggtaaca gcttgcaata cttgagcaga aatggcagat
    16741 ttatcaagga aaaagttaac gtaaggtcct gttgcgacaa ctttttcaaa ggcttggctg
    16801 ttcatttttt cagccagttc agccgcaatc atttgtggtg ctttacgttc gacttttgca
    16861 agagaaaaag cagggaaagc aatgtctccc atttctgagt ttttaggggt ttccagtaac
    16921 tttaaaacag cctcttggtc caggctatca atgatgctag ataattcgct agcaatcaat
    16981 tcttttatat tcattaagag ctcctttttg gacttttcta ctattttatc acaattttaa
    17041 agaaagaaga aaaaattttt gaaatctcct gtttttttgg tataatatgg ttataaatat
    17101 agttataaat atgcacgcaa gaggatttta tgagaaaaag agatcgtcat cagttaataa
    17161 aaaaaatgat tactgaggag aaattaagta cacaaaaaga aattcaagat cggttggagg
    17221 cgcacaatgt ttgtgtgacg cagacaacct tgtctcgtga tttgcgcgaa atcggcttga
    17281 ccaaggtcaa gaaaaatgat atggtgtatt atgtactagt aaatgagaca gaaaagattg
    17341 atttggtgga atttttgtct catcatttag aaggtgttgc aagagcagag tttaccttgg
    17401 tgcttcatac caaattggga gaagcctctg ttttggcaaa tattgtagat gtaaacaagg
    17461 atgaatggat tttaggaaca gttgctggtg ccaatacctt attggttatt tgtcgagatc
    17521 agcacgttgc caaactcatg gaagatcgtt tgctagattt gatgaaagat aagtaaggtc
    17581 ttgggagttg ctctcaagac ttatttttga aaaggagaga cagaaaatgg cgatagaaaa
    17641 gctatcaccc ggcatgcaac agtatgtgga tattaaaaag caatatccag atgctttttt
    17701 gctctttcga atgggtgatt tttatgaatt attttatgag gatgcggtca atgctgcgca
    17761 gattctggaa atttccttaa cgagtcgcaa caagaatgcc gacaatccga tccctatggc
    17821 gggtgttccc tatcattctg cccaacagta tatcgatgtc ttgattgagc agggttataa
    17881 ggtggctatc gcagagcaga tggaagatcc taaacaagca gttggggttg ttaaacgaga
    17941 ggttgttcag gtcattacgc cagggacagt ggtcgatagc agtaagccgg acagtcagaa
    18001 taattttttg gtttccatag actgcgaagg caatcaattt ggcctagctt atatggattt
    18061 ggtgacgggt gacttttatg tgacaggtct tttggatttc acgctggttt gtggggaaat
    18121 ccgtaacctc aaggctcgag aagtggtgtt gggttatgac ttgtctgagg aagaagaaca
    18181 aatcctcagc cgccagatga atctggtact ctcttatgaa aaagaaagct ttgaagacct
    18241 tcatttattg gatttgcgat tggcaacggt ggagcaaacg gcatctagta agctgctcca
    18301 gtatgttcat cggactcaga tgagggaatt gaaccacctc aaacctgtta tccgctacga
    18361 aattaaggat ttcttgcaga tggattatgc gaccaaggct agtctggatt tggttgagaa
    18421 tgctcgctca ggtaagaaac aaggcagtct tttctggctt ttggatgaaa ccaaaacggc
    18481 tatggggatg cgtctcttgc gttcttggat tcatcgcccc ttgattgata aggaacgaat
    18541 cgtccaacgt caagaagtag tgcaggtctt tctcgaccat ttctttgagc gtagtgactt
    18601 gacagacagt ctcaagggtg tttatgacat tgagcgcttg gctagtcgtg tttcttttgg
    18661 caaaaccaat ccaaaggatc tcttgcagtt ggcgactacc ttgtctagtg tgccacggat
    18721 tcgtgcgatt ttagaaggga tggagcaacc tactctagcc tatctcatcg cacaactgga
    18781 tgcaatccct gagttggaga gtttgattag cgcagcgatt gctcctgaag ctcctcatgt
    18841 gattacagat gggggaatta tccggactgg atttgatgag actttagaca agtatcgttg
    18901 cgttctcaga gaagggacta gctggattgc tgagattgag gctaaggagc gagaaaactc
    18961 tggtatcagc acgctcaaga ttgactacaa taaaaaggat ggctactatt ttcatgtgac
    19021 caattcgcaa ctaggaaatg tgccagccca ctttttccgc aaggcgacgc tgaaaaactc
    19081 agaacgcttt ggaaccgaag aattagcccg tatcgaggga gatatgcttg aggcgcgtga
    19141 gaagtcagcc aacctcgaat acgaaatatt tatgcgcatt cgtgaagagg tcggcaagta
    19201 catccagcgt ttacaagctc tagcccaagg aattgcgacg gttgatgtct tacagagtct
    19261 ggcggttgtg gctgaaaccc agcatttgat tcgacctgag tttggtgacg attcacaaat
    19321 tgatatccgg aaagggcgcc atgctgtcgt tgaaaaggtt atgggggctc agacctatat
    19381 tccaaatacg attcagatgg cagaagatac cagtattcaa ctggttacag ggccaaacat
    19441 gagtgggaag tctacctata tgcgtcagtt agccatgacg gcggttatgg cccagctggg
    19501 ttcctatgtt cctgctgaaa gcgcccattt accgattttt gatgcgattt ttacccgtat
    19561 cggagcagca gatgacttgg tttcgggtca gtcaaccttt atggtggaga tgatggaggc
    19621 caataatgcc atttcgcatg cgaccaagaa ctctctcatt ctctttgatg aattgggacg
    19681 tggaactgca acttatgacg ggatggctct tgctcagtcc atcatcgaat atatccatga
    19741 gcacatcgga gctaagaccc tctttgcgac ccactaccat gagttgacta gtctggagtc
    19801 tagtttacaa cacttggtca atgtccacgt ggcaactttg gagcaggatg ggcaggtcac
    19861 cttccttcac aagattgaac cgggaccagc tgataaatcc tacggtatcc atgttgccaa
    19921 gattgctggc ttgccagcag accttttagc aagggcggat aagattttga ctcagctaga
    19981 gaatcaagga acagagagtc ctcctcccat gagacaaact agtgctgtca ctgaacagat
    20041 ttcactcttt gatagggcag aagagcatcc tatcctagca gaattagcta aactggatgt
    20101 gtataatatg acacctatgc aggttatgaa tgtcttagta gagttaaaac agaaactata
    20161 aaaccaagac tcactagtta atctagctgt atcaaggaga cttctttgac aattctccac
    20221 ttttttgcta gaataacatc acacaaacag aatgaaaagg agctgacgca ttgtcgctcc
    20281 cttttgtcta ttttttaagg agaaagtatg ctgattcaga aaataaaaac ctacaagtgg
    20341 caggccttgg cttcgctcct gatgacaggc ttgatggttg ctagttcact tctgcaaccg
    20401 cgttatctgc aggaagtatt agacgccctc cttactggga aatatgaagc tatttatagt
    20461 atcggggctt ggttgattgg tgtggccgta gtcggtctag ttgctggtgg actcaatgtt
    20521 gtcctcgcag cctatattgc ccaaggagtt tcatccgacc ttcgggagga tgccttccgt
    20581 aaaattcaaa ccttttctta tgctgatatt gaacaattta atgcgggaaa tctagtcgtt
    20641 cgaatgacaa atgatatcaa ccagattcag aacgttgtca tgatgacctt ccaaattctt
    20701 ttcagacttc ccctcttgtt catcggttcg tttatcctag cggttcaaac cttaccttct
    20761 ctgtggtggg tgattgttct catggtagtc ttgatttttg gtttgactgc tgtcatgatg
    20821 ggaatgatgg ggcctcgttt tgccaagttt caaacccttc ttgagcgcat caatgccatt
    20881 gccaaggaaa atttacgtgg cgttcgtgtg gtcaagtcct ttgtccaaga aaaagagcaa
    20941 tttgctaagt ttacagaggt ctcagacgag ctttttggtc aaaaccttta cattggttat
    21001 gccttttcag tagtggaacc ctttatgatg ttggttggtt acggggcggt cttcctctct
    21061 atttggctgg tcgcgggaat ggttcagtcg gatccgtctg ttgttggttc catcgcttct
    21121 tttgttaatt acctaagcca gattatcttt accattgtta tggttggatt tttgggaaat
    21181 tctgtcagcc gtgccatgat ttccatgcgt cgtattcgag aaattcttga cgcagagcca
    21241 gctatgacct tcaaggatat cccagatgaa gagttggttg gaagccttag ctttgaaaat
    21301 gtgaccttta cctatccaat ggacaaggaa ccgatgctga aagatgtgag ctttactatt
    21361 gaacctggtc aaatggttgg tgtagttgga gcgactggtg caggaaagtc aaccttggct
    21421 caattgattc cacgtctctt tgatccacag aacggggcca ttaaaatcgg tggcaaggat
    21481 attcgagaag tgagtgaagg aaccctgcgt aaaacagttt ccatcgttct ccaacgtgcc
    21541 attcttttta gtggaacgat tgcagataac ttgagacagg ggaagggaaa tgctactcta
    21601 tttgaaatgg agcgcgcagc caatattgcc caggctagtg aattcattca tcgtatggag
    21661 aaaacctttg aaagtccagt tgaagaacgg ggaaccaatt tctctggtgg acaaaaacaa
    21721 aggatgtcga ttgcgcgtgg gattgtcagc aatccacgta ttctgatttt tgacgattcg
    21781 acctcagcct tggatgccaa atcagagcgc ttggtgcaag aagctttgaa taaggacttg
    21841 aaggggacga caaccattat tattgctcaa aaaattagct cggttgtcca tgcagacaag
    21901 atcttggttc taaatcaagg acgattgatt ggtcaaggca cgcatgcaga cttggttgcc
    21961 aacaatgccg tttaccgtga aatctatgaa acacagaaat gaaagacaaa ctataagaaa
    22021 agtcaatagt tttatctaaa ttatttctta tttcaatttg atgatttggc gatgatttta
    22081 gagcacggca aaaagccctt gaaaaagtcc attttttcaa aggtaatcct gtgttgattt
    22141 cagaaattac atcacttttt gttcgtcaaa tggcagctct tttttaggat ataaaacagg
    22201 gttcggataa gtttttttgc aaggtggatg atggctacat tgtaatgttt tccttattct
    22261 aacttagtct taagataggc cttaaaagca ggtgaaaagc gagggcatgc tttggcagct
    22321 tgtatgagtg cctaccgcag atgaggggaa ccccgtttga tcattcttcc agctaaatca
    22381 atctgatctg actgataaat agaagaatcc agtccagcga aagcttgtaa ttgagcaaga
    22441 ttatcaaagg catgaatatt tcgaatctcg gctaaaatga ccgcccctaa acgattccca
    22501 atcccagtaa ccgtcgtgat gatcgagttg aactcagcca tcaagtcatt gacacatgtt
    22561 tccgccttgt caatgagcct cttgtaatgt ttgatgtttt cattacacga gataaaacgt
    22621 ttatgcgtta tcaaactcat gaccaattga aacaaaaagc tgtggttaga tcctttcgga
    22681 aattgtcaag cgattggagg aaatgaacta atccacagcg gcttattcca agtataccac
    22741 ttgggctttg gcagtagcta actgcgctaa atataatata aggaggagta aaatgaagac
    22801 agttcaattt ttttggcatt attttaaggt ctacaagttc tcatttgtag ttgtcatcct
    22861 gatgattgtt ctggcgactt ttgcccaagc cctctttcca gtcttttctg gacaagcggt
    22921 gacgcagcta gccaatttag ttcaagctta tcaaaatggc aatccagaac ttgtatggca
    22981 aagcctatca ggaatcatgg tcaatcttgg cctgctggtt ttggttctat ttatctctag
    23041 tgtaatatac atgtgtctca tgacgcgcgt gattgcagaa tcgaccaacg agatgcgcaa
    23101 aggcctcttt ggtaagcttg ctcagttgac ggtttctttc tttgaccgtc gacaagatgg
    23161 cgatatcctg tctcatttta ccagtgattt ggataatatc ctccaagcct ttaacgaaag
    23221 cttgattcag gtcatgagca atattgtttt atacattggt ctgattcttg tcatgttttc
    23281 gagaaatgtg acgctggctc tcatcaccat tgccagcacc ccattggctt tccttatgct
    23341 gattttcatc gtgaaaatgg cacgcaaata caccaacctc cagcagaaag aggtagggaa
    23401 gctcaacgcc tatatggatg agagcatctc aggccaaaaa gccgtgattg tgcaaggaat
    23461 tcaagaggat atgatggcag gatttcttga acaaaatgag cgcgtgcgca aggcaacctt
    23521 taaaggaaga atgttctcag gaattctttt ccctgtcatg aatgggatga gcctgattaa
    23581 tacagccatc gtcatctttg ctggttcggc tgtacttttg aatgataagt ctattgaaac
    23641 aagtacagcc ctaggtttga ttgttatgtt tgcacaattt tcacagcagt actaccagcc
    23701 tattatccaa gttgcagcga gttggggaag ccttcagttg gcctttactg gagctgaacg
    23761 aattcaggaa atgtttgatg cagaggagga aatccgacct gaaaaggctc caaccttcac
    23821 taagttgcaa gaaagtgttg aaatcagtca tatcgatttt tcatacttgc ctgataaacc
    23881 tattttgaaa gatgtcagca tttctgcccc taaaggccag atgacagcag ttgttgggcc
    23941 gacaggttca ggaaaaacga ctattatgaa cctcatcaat cgcttttatg atgttgatgc
    24001 tggtggtatt tattttgatg gtaaagacat tcgtggctat gacttagata gtcttagaag
    24061 caaggtggga attgtattgc aagattcggt cttgtttagc ggaacgatta gagacaatat
    24121 ccgatttggt gtgccagatg ctagtcagga aatggttgag gtagcagcaa aagcaaccca
    24181 cattcacgac tatatcgaaa gtttgcctga taagtacgat actcttattg atgatgacca
    24241 gagcatcttt tcaacagggc agaagcaatt gatttcaatc gctcgaaccc tgatgataga
    24301 tccagaagtt ctcattctcg atgaagcaac ttcaaacgta gatacggtga cagaaagcaa
    24361 gattcagcat gccatggagg tggttgtagc aggtagaact agtttcgtca ttgcccaccg
    24421 cttgaaaacc attctcaatg cagatcagat tattgtcctt aaagatggag aagtcattga
    24481 acgtggtaac caccatgaac ttttgaagct aggtggcttt tattcagaac tctatcacaa
    24541 tcaatttgtt ttcgaataag aaagaagttg tcctatgtgg gcagcttttt cttgtccata
    24601 aaaaatgttt atcacagcct taaaaaaaac atattagacg aaagtcattt tgagtgatat
    24661 gataggacta tcgttagcat tcgaaaggag aggcatcatg gctagaacgg ttgtaggagt
    24721 tgctgcaaat ctatgtcccg tagacgcaga aggcaaaatc attcattcat ctgtatcttg
    24781 tagattcgca gagatcattc gtcaagtcgg tggtctccct ttagtcattc ctgttggtga
    24841 tgagtcagtt gtacgtgatt atgtggaaat gattgacaaa ctcattttga caggaggcca
    24901 aaatgttcat cctcagtttt atggagagaa gaagaccgtc gagagcgatg attacaatct
    24961 ggtccgtgac gaatttgaat tggcactctt gaaggaagcg cttcgtcaga ataaaccaat
    25021 tatggcaatc tgtcgcggtg tccaacttgt caatgttgcc tttggtggaa ccctcaatca
    25081 agaaatcgaa ggtcactggc aaggactacc tttcgggaca tctcactcta ttgagacagt
    25141 agaaggaagc gtggtggcta agctatttgg gaaagaaagt caggtcaact cagtccatcg
    25201 tcaaagcatt aaagatttgg cacctaattt ccgtgtaact gctattgatt caagagacca
    25261 aactattgaa gcgattgagt ctatcgacga gcaccgcatt atcggtttac agtggcatcc
    25321 agagtttctg gttaatgaag aagatggcaa tttagaatta tttgagtatt tattgaatga
    25381 actgtaacga ttggaacatc tggtcgttct ttcttttatt ttttcaaaat ttttagaatg
    25441 tggcatttta cgcaaacgtt tgaattctga taaaaattgg agaaattcga caaaaaaact
    25501 tgaaaaaaac gaaggtaagc gttatgatag aaaagaagaa atattggagg aagaacatgt
    25561 cacatattaa atttgattat tcaaaagttt tagacaaatt tgttgcacca catgaagtgg
    25621 aatacatgca atcacaagta acagcagcag atgaattgat ccgtaaagga actggtgctg
    25681 gtagcgactt cttgggatgg ttggaccttc ctgaaaaata cgatcgcgaa gaatttgacc
    25741 gcatcttgaa agctgctgag caaatcaagt cagacagcga tgttttggtt gtgatcggta
    25801 ttggtggatc ttaccttggt gcaaaagcag caatcgactt cttgaatcac cattttgcta
    25861 acttgcaaac aaaagaagaa cgcaaagctc cacaaatcct ttacgcagga aactcaatct
    25921 catctactta ccttgctgac ttggtagagt atgttgcaga caaagacttc tcagtaaacg
    25981 taatttctaa atcaggtaca acaactgaac cagcgattgc tttccgtgtc tttaaagaac
    26041 tcttggttaa gaaatacggt caagaagaag ctaacaaacg tatctatgca acaactgacc
    26101 gccaaaaggg tgctgttaag gttgaagcag acgctaacgg ttggggaaca tttgttgttc
    26161 cagatgatat cggtggacgc ttctcagtat tgacagccgt tggtttgctt ccaatcgcag
    26221 catcaggagc tgacataaaa gctcttatgg aaggtgcgaa tgcagctcgc aaagactaca
    26281 cttcagacaa aatctctgaa aacgaagctt accaatacgc agctgttcgt aacatccttt
    26341 atcgtaaagg ctatgcaact gagatcttgg taaactatga gccatcactt caatacttct
    26401 cagaatggtg gaaacaattg gctggtgaat cagaaggaaa agaccaaaaa ggtatctacc
    26461 caacttcagc caacttctca actgacttgc actcacttgg tcaatttatc caagaaggaa
    26521 ctcgtatcat gtttgaaaca gttgtccgtg ttgacaaacc tcgtaaaaac gtgcttattc
    26581 ctactttgga agaagacctt gacggacttg gttaccttca aggaaaagac gttaactttg
    26641 taaacaaaaa agcaactgac ggtgttcttc ttgcccacac agatggtgat gtaccaaaca
    26701 tgtatgtgac tcttccagag caagacgctt tcactcttgg ttacactatc tacttcttcg
    26761 aattggcaat tgccctttca ggttacttga atgctatcaa cccatttgac caaccaggtg
    26821 ttgaagctta taaacgtaac atgtttgccc ttcttggaaa accaggattt gaagaattga
    26881 gcaaagaact taacgcacgt ctataataga agaaaagagt ggtttgccca ctctttttac
    26941 tctctttatc catagaaatt ggactcagcc aagacttgtg atataatata gaaagcaaaa
    27001 aggcagacgc ctagataata ggagaaacta tgtcaaaaga tatccgcgta cgttacgcac
    27061 caagtccaac aggactacta cacatcggaa atgctcgtac agcattgttt aattacttgt
    27121 atgcgcgcca tcatggtgga acatttctca tccgtatcga agatactgac cgtaaacgcc
    27181 atgtcgagga tggtgaacgt tcacaacttg aaaaccttcg ctggttaggc atggattggg
    27241 atgaaagtcc agaatcacat gagaattatc gccagtctga gcgtttggac ttgtatcaaa
    27301 aatacatcga ccaactatta gctgaaggaa aagcctacaa atcttacgtt acagaagaag
    27361 agttggtagc tgaacgtgaa cgtcaagaag tagctggcga aacaccacgc tacatcaatg
    27421 aataccttgg tatgagtgaa gaagaaaaag cagcttacat cgcagaacgt gaagcagcag
    27481 ggatcatccc aactgttcgt ttggctgtca atgagtcagg tatctacaag tggcatgata
    27541 tggtcaaagg cgatatcgaa tttgaaggtg gcaatatcgg tggtgactgg gttatccaaa
    27601 agaaagacgg ttacccaact tacaactttg ccgttgttat cgatgaccac gatatgcaaa
    27661 tctctcatgt tatccgtgga gatgaccata ttgctaatac accaaaacag cttatggtct
    27721 atgaagctct tggttgggaa gctccagagt tcggtcacat gaccttgatt atcaactctg
    27781 agactggtaa gaagttatct aaacgtgata ctaatacact tcaatttatc gaagattacc
    27841 gaaagaaagg ttaccttcca gaagcagtct ttaattttat tgctcttctt ggttggaacc
    27901 caggtggtga agatgagatt ttctctcgtg aagaactcat taaacttttt gatgaaaacc
    27961 gcctcagcaa gtcaccagca gcctttgatc agaaaaaact agactggatg agcaatgatt
    28021 atatcaagaa tgcagacctt gaaaccatct ttgaaatggc aaaaccattc ttagaggaag
    28081 caggccgttt gactgacaag gctgaaaaat tagttgagct ctataaacca caaatgaaat
    28141 cagtagatga gattatccca ttgacagatc ttttcttctc agatttccca gaattgacag
    28201 aagcagagcg cgaagtcatg acgggtgaaa cagttccaac agttcttgaa gcattcaaag
    28261 caaaacttga agcgatgaca gatgataaat ttgtgacaga aaatatcttc ccacaaatta
    28321 aagcagttca aaaagaaaca ggtattaaag ggaaaaatct tttcatgcct attcgtatcg
    28381 cagtttcagg cgaaatgcat gggccagaat taccagatac aattttcttg cttggacgtg
    28441 aaaaatcaat tcagcatatc gaaaacatgc taaaagaaat ctctaaataa agaggataca
    28501 ataatggaca tctgggaaaa gatgtacgaa gaagcacaga aactatacaa tccacatgaa
    28561 gtatcagact ttgtttatgc caatcatgtt gtagctgcag tagaagcaga agatggacaa
    28621 atatttacag gattctgtat ggagggaacc tgtggtgttt tccatctctg cgcagaacgg
    28681 gcggccctct tcaatatgta ccaattttca ggacaaacta aggttaagaa agtattagcc
    28741 tttcgagaca aaccacctta tggtggaagt tcagccatgc cttgcggtgc ttgtagagaa
    28801 ttccttttag agcttaatgc tgaaaataaa gatgcagaat tcatgatgga ctataatata
    28861 agaaaaacag ttaaagtcgc agaactaatc ccttattggt ggggagaaga acgtgcttct
    28921 aagtttaatg agcgatagaa gagtcagtat aattctggct ttttttacta taagttgaaa
    28981 aaata
//
LOCUS       DAVKGC010000011        28183 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKGC010000011 DAVKGC010000000
VERSION     DAVKGC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28183
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.011"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            208..351
                     /locus_tag="VW049_000424"
     CDS             208..351
                     /locus_tag="VW049_000424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431602.1"
                     /translation="MADRTDEVSSKHRFEVADRTDEVSSKHRFEVADRTDEVSNIYTA
                     RRS"
     gene            complement(705..1685)
                     /locus_tag="VW049_000425"
     CDS             complement(705..1685)
                     /locus_tag="VW049_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9431603.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(2001..4016)
                     /gene="recG"
                     /locus_tag="VW049_000426"
     CDS             complement(2001..4016)
                     /gene="recG"
                     /locus_tag="VW049_000426"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9431604.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRIQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTAHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(4035..5138)
                     /locus_tag="VW049_000427"
     CDS             complement(4035..5138)
                     /locus_tag="VW049_000427"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9431605.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPSGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(5128..5496)
                     /locus_tag="VW049_000428"
     CDS             complement(5128..5496)
                     /locus_tag="VW049_000428"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9431606.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5535..6566)
                     /locus_tag="VW049_000429"
     CDS             complement(5535..6566)
                     /locus_tag="VW049_000429"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9431607.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6568..7599)
                     /locus_tag="VW049_000430"
     CDS             complement(6568..7599)
                     /locus_tag="VW049_000430"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9431608.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7680..10193)
                     /gene="secA"
                     /locus_tag="VW049_000431"
     CDS             complement(7680..10193)
                     /gene="secA"
                     /locus_tag="VW049_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9431609.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMTALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(10339..10626)
                     /locus_tag="VW049_000432"
     CDS             complement(10339..10626)
                     /locus_tag="VW049_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431610.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDKPLLGIRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITLAILPIFGN"
     gene            complement(10654..11286)
                     /locus_tag="VW049_000433"
     CDS             complement(10654..11286)
                     /locus_tag="VW049_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9431611.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(11290..11931)
                     /locus_tag="VW049_000434"
     CDS             complement(11290..11931)
                     /locus_tag="VW049_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431612.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEVNLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11924..12715)
                     /locus_tag="VW049_000435"
     CDS             complement(11924..12715)
                     /locus_tag="VW049_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431613.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAILTHFFGTFSPLGWNSLFSDGSSLQR
                     LLDGEITSYLFFTCVLLIGIFINAVYFLQIVDYLGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVSYMVLEKYEDNV"
     gene            complement(12900..13235)
                     /locus_tag="VW049_000436"
     CDS             complement(12900..13235)
                     /locus_tag="VW049_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431614.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(13375..13710)
                     /locus_tag="VW049_000437"
     CDS             complement(13375..13710)
                     /locus_tag="VW049_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431615.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13799..15109)
                     /gene="der"
                     /locus_tag="VW049_000438"
     CDS             complement(13799..15109)
                     /gene="der"
                     /locus_tag="VW049_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9431616.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(15123..15836)
                     /locus_tag="VW049_000439"
     CDS             complement(15123..15836)
                     /locus_tag="VW049_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9431617.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMAL
                     GVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15833..16729)
                     /gene="dnaI"
                     /locus_tag="VW049_000440"
     CDS             complement(15833..16729)
                     /gene="dnaI"
                     /locus_tag="VW049_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9431618.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16730..17899)
                     /locus_tag="VW049_000441"
     CDS             complement(16730..17899)
                     /locus_tag="VW049_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9431619.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLVLHAPLSSQD
                     FLEHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKIQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17900..18373)
                     /gene="nrdR"
                     /locus_tag="VW049_000442"
     CDS             complement(17900..18373)
                     /gene="nrdR"
                     /locus_tag="VW049_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9431620.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            18513..18878
                     /locus_tag="VW049_000443"
     CDS             18513..18878
                     /locus_tag="VW049_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9431621.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18883..19578
                     /locus_tag="VW049_000444"
     CDS             18883..19578
                     /locus_tag="VW049_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431622.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19595..20362
                     /locus_tag="VW049_000445"
     CDS             19595..20362
                     /locus_tag="VW049_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431623.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20715..21914)
                     /locus_tag="VW049_000446"
     CDS             complement(20715..21914)
                     /locus_tag="VW049_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9431624.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILVHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21911..22804)
                     /locus_tag="VW049_000447"
     CDS             complement(21911..22804)
                     /locus_tag="VW049_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431625.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLADKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            22921..23001
                     /locus_tag="VW049_000448"
                     /pseudo
     CDS             22921..23001
                     /locus_tag="VW049_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            23486..23699
                     /locus_tag="VW049_000449"
                     /pseudo
     CDS             23486..23699
                     /locus_tag="VW049_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            24114..24731
                     /locus_tag="VW049_000450"
     CDS             24114..24731
                     /locus_tag="VW049_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9431626.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(25051..25938)
                     /locus_tag="VW049_000451"
     CDS             complement(25051..25938)
                     /locus_tag="VW049_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9431627.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(26085..27968)
                     /locus_tag="VW049_000452"
     CDS             complement(26085..27968)
                     /locus_tag="VW049_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9431628.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSIPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
BASE COUNT         8323 a         6266 c         5273 g         8321 t
ORIGIN      
        1 tttcaaaaaa ttcttttata taataatgtc cttgaaagta gtttcagctg aacatttata
       61 gtccataaaa agaaaacaga aactcatctg tttttatact caatgaaaat caaagagcaa
      121 actaagaagc tagccgcatg atgctcaaaa cactgttttg aggttgtaga taagactgac
      181 aaagtcagcc caaaacaccg ttttgaggtg gcagatagaa ctgacgaagt cagctcaaaa
      241 caccgttttg aggtggcaga tagaactgac gaagtcagct caaaacaccg ttttgaggtg
      301 gcagatagaa ctgacgaagt cagtaacata tatacggcaa ggcgaagctg acgtggttta
      361 aagagatttt tgaaaagtat taacacctac agacaagcct gacgatagca agaactaccc
      421 tactcgatag gtatcggctt ttgctttctg aaaaaaatta ttttaagcaa tttgacaaat
      481 ctagcaacaa aaaattctat aaatataata gattgaaact agaatagtac acatttgctt
      541 ctaaaacatt gttagaaatc gatttgactg tcctgatcgt tttgtcctat tcttgtttca
      601 ttttactata tttccatgat aaaacgcata gtatcaagtt tttttaatcc cctgatacta
      661 tgcgtgtttg taatttttaa gattttgtgc ttagagtcga ctccttattt tagatattta
      721 aaaggaatct cacttccaca gagccagttg tagacttggt cattgacaaa tacattcatg
      781 gcttcgtgag catactcagg catgatgcga taggttttat cgcaggtcag acgattataa
      841 atcgcaaact gggtaatggg atagcaaaca tcgtcgtcca agcccgtaat catcttaacc
      901 tcaccttgga tacgatgggc aagatttttg acatcgatat aggcaagggt cgccatgatt
      961 tcctcctctg tttcatggaa ggggtcgtga aacttgaaat aacggaaaag ttcgtcgtaa
     1021 gcctcgctag tattaccaat ctcaatcacc cgtctgaagt ctgacaagaa gggataaatg
     1081 gcaactgttt tctgaattcg aggattgagc gctgctgcaa ctagagctag agcccctcct
     1141 tgtgaggcac catagctaga aagacgcttc tcatcaacct gagacagact agcaacaatt
     1201 tcgaccaact ggtaaatatc cagataaaca tccttataaa agaggtggtc ccgaccttcc
     1261 atagcaccac ggataatatg ccccttcacg gtatttccta aaggagaacg caagccgtct
     1321 tgtgagtaac ctgactggcc ccgcacatcc atggaaacaa caccgtaacc agctacggtg
     1381 aagcccagca tgtcggccca gtcccagcca cgtcccatat aaccatgaaa atggaagatt
     1441 agtggaacct tctcctcact ctttggaaga acaatgcgtg catagacctt tccttccttg
     1501 cttccttcaa atgttaactc atagcacttg acttgaggaa tgtggaaatc tctttcctcc
     1561 aagtggtagg atggaagcgt ggaaacattt ttcacttccc catcccagaa agcatcaaag
     1621 tcttccggaa cctcatccct tcctctatag gtcttaattt cttctagcaa agctggattt
     1681 ttcatagcat gctcctttta ttttgtaatc gttaccacaa ctgtagcata tctatgtaat
     1741 caatgcaaga aagaaggata aatagaaagt gaatttagat tctttacttt taggatgatg
     1801 ggctgaaagt agttttagaa acgaaaaaga gcatttcgct ctctcttctg tttatactag
     1861 ttttttgctt ccttcttttg gaaagtggtt tggtaattta ctttaatcgt tactgtcata
     1921 tgagagtcct cgtttctttt tgatgacttt agtataagag ccaaacctaa aaactagctt
     1981 aagttttcct tagagaaagc ttaatccaga tgttctttct tttccagatg aagggcaatc
     2041 atgcgccact ctggatcttc ttgccaagct tctatagaac taatgtagct agcaaccttt
     2101 cttgcttctt ctaaaatcgg aaaatcttcg ataatatcag ccacttggaa ctctggaagt
     2161 cctgactgtc tggttccaaa aatctcacca gaaccacgca ttttcaaatc ttcctccgca
     2221 aggacaaatc cattggtcgt ttctgtcatg atgcgcatgc ggtctttccc agaatccgtc
     2281 ttgggattgg caacgagaac agcgtaggac tgcttgtccc cccgaccgac acgacctcta
     2341 agctggtgaa gctggctgag cccgaagcga tcggcatcca tgataatcat gacagtcgca
     2401 ttgggaacgt tgaccccaac ctcaataacc gtcgtcgaaa ccagaatatc cgtctttctc
     2461 tccttgaaat cctgcatgat ctggtctttt tcgtcactct tcatcctacc atgtagaaga
     2521 gccacctctg ccttgcctgc aaaatgagcc gtcagctctt ctgataaggc aatggcattt
     2581 ttcaaatcta gagcttctga ttcttcaatc aaaggagaga tgacataggc ttgggaacct
     2641 ttttgaattt ccccctctaa ccaagtcaag acctgaggta gttgctcatg tttgatccag
     2701 cgcgtcacaa taggcttccg acctgctggc atctggtcga taatggaaac atccatatct
     2761 ccaaaggctg tgatggcaag cgtccgtgga atgggagtcg ccgtcatcat gaggacatct
     2821 ggattgtcac ctttttcccg taaaatacgc ctttgcccta caccaaaacg gtgctgctca
     2881 tcgataataa tcaaaccaag acgagcatac tccaccccat cttgtatcag agcgtgagtt
     2941 cctataatca aatcagcctc acccttggca atggtctcca agacttctct cttttctgca
     3001 gctttcaagg aacctgtcaa gagagccagt ttcaaattgg gaaaaaggtt ctgtaaactc
     3061 tcaaagtgtt gctctgcgag gatttctgtt ggtaccatta gggcagcctg ataacctgct
     3121 gtcactgccg caaacatggc caagccagcg actaccgttt ttccactccc cacatcccct
     3181 tgtaggagac gattcatgtg gtggtcggac ttcatatcag ttaaaatttc ctgcaaactc
     3241 ttttcctgag cttgggtcag ggcaaaagga agacttgctt taactgctgt cactttttcc
     3301 tgagaccaat ccagaaccag accacttccc tgaattctat tttcagactt gagcgtctgc
     3361 agctgcattt ggaaataaaa gagttcctca aactttatac ggcgaagagc ctgcttgtat
     3421 tctgccaaat cctttggaaa atgcatagct cggactgcct gacaacggga catgagtttg
     3481 tatttgtcta gtaaagactg gggcagattt tcttctatca agaggtccag tccctgatca
     3541 aaagccgtct tgatgacctt aaccagactg gcctgactga ttccctgagc cagacgataa
     3601 acaggctgga ggtcatcttc tacctgagcc agaaccttca tcccagtcag actagcctta
     3661 gcgcggtccc attttccaaa gacagcaagg gttgctccca actctatttt atcagccaga
     3721 tagggctggt taaagaaatt caccgcaaaa acgacctctc cctgcttgag actaaaacgc
     3781 aggcgattgc gcttgaaacc ataatactgg acactagcag gagtcactac ctgaccagaa
     3841 agaactgcct tctcaccgtc ttccagctcc agcacctgct tggttttgaa gtcttcataa
     3901 cggaaaggaa agtagagcaa gagatcttgc aagttttcaa ttcctagttt ggcgtatttt
     3961 tctgctgact ttggtcccac accaggcaag acatgcaagg gttgatgtag attcatgctc
     4021 cactcctttc ttttctaata atattctctc ggaatacggt cgctgaggag gcaaaccacc
     4081 tcatagttaa tggttacgcg gtaggtcgct acctgagttg cagtgatttc cttatcccca
     4141 ttggagccaa tcaaggttac cttggttccg agtgggtaag gcttaggcaa tcgaatagtg
     4201 atttggtcca tcgaaaccct gccgacaatt gggcaagctt ggccatctac caagacagag
     4261 aaattttgca tgtctcttgt ccatccatct gcatacccga ttggcacggt cgcgatgact
     4321 tgctcgctat ccgcttgata agttgctcca tatcccatgc aagctccaga tggaactgtc
     4381 ttgacatgaa ccagagcaga ctccaaggtc aaggccggta tcaaatcata aggcaaatcc
     4441 aagaccgctc cacttggatt gaggccatac atggcatctc ccatacgaac cgcattgaaa
     4501 atagtctcta catgccaaag agtcgttgca gaattgctag catgaaccag ctctggaact
     4561 tccttcatac tagctaaaat agttttaaac cgttctaact gggcattaaa atagtcatct
     4621 gattcctcat cagcagtagc aaagtgggta aagattcctt caacacgaac accgtgttgt
     4681 tggagcaaat cttgagcctg ctcaacctca ctggcctctc taaaaccaat ccgtcccatc
     4741 cctgaatcaa tcttgaggtg gactgtcaat ccagttaggt ccacttcctt atctaagagt
     4801 gcttgaatcc actccagtcc agccactgtc aaggtgaagt catattcttt agctagagca
     4861 acagcttcga tttcagaaac tcctaaaatg agaattggct tgctgagtcc agcttgtctg
     4921 agttcaatgg cttcatcgat attggaaacg caaaagccat caacatcatc ttgaattgcc
     4981 ttggcaacgg caacagctcc atgaccataa gcattggcct taaccacagc caacttgagc
     5041 gttccttgag ggatatgagc ccccatttgc tgaatatttt gtcgaatagc tcccagatga
     5101 atcagagcct tggttggtct atgtggacta gctttcatga ttttcctcca aaatgacact
     5161 ggctgtcaca aactgatcgg tgtggctgat agacagccaa atctttcctg aaaatggtgc
     5221 ctgactaaaa taaggcgccc cacgttcatt gttcaagact tccaaatcct gaaaaccgag
     5281 cttgctaatg cccgttccca tagccttgga aaaggcctcc ttagccgacc agcgaccagc
     5341 taaatattct atttgcctgc gtcctttgag actggtgaag cgctccattt cctgagcggt
     5401 cagtacacgc ttagcaaatc cttcatgtcg tgtaactgca ctttctatcg aagccaattc
     5461 ttcgatgtca attccgtgtc caactatcat tctcatcaaa aggagttgag attccccaac
     5521 tccatccttt tcacttattt tgtcaaggta acataaatct cttctaccaa ggcctctgta
     5581 ttttcccaac ctaggcaagg gtcagtaata gagcaaccaa agatctctgg ttggttttga
     5641 cgaccatctg ctaggtaaga ttcaatcata aatcctcgaa ccgtcttttt aattttctca
     5701 ttccaatcac gattctgcaa ggtctggcga acaattcgaa tctgctccat atattgcttg
     5761 cctgagttat catggttggt gtcaatgagg ataaaaggat tttcaagtcc catggtttca
     5821 tagcgttcaa tggcttggag taggttttca tagtagtaat tcggcatata attgccatac
     5881 tcgttgactg ctccacggag gataacatgg gccaaaggat tacctgatgt ctcaacttcc
     5941 tgcccatgat aaaggaaggt ttgcttgttt tgagcagcat agatggcgtt aaacataaca
     6001 cccaaatttc ctgaggttgg atttttcatc cctactggtg catcaatccc agaagccaca
     6061 aagcggtgct cttggtcttc cacagaacga gctccaacgg catggtagct gaccaagtca
     6121 tccaccaaga tcagatttga cggataaagc atctcatctg ccgttgtcaa accagtctct
     6181 gtaatcacgc ggtagtgcaa ctggcgcaca gcctgcaagc cattaatcag gcttggagcc
     6241 ttagaagtat ctggctggtg aactaatcct ttatagccgt ctccattggt acgaggctta
     6301 gcagtataca cgcgcatgac catgaaaatc ttgtccgcta ccttcttttg caaggcggat
     6361 aaacggcgag catattccaa gaccgcctct tcattatcag aagagcaagg accaatcacc
     6421 aagagaatac ggtcatcttc ccctgaaata atatctgcca attccctgtc acggctttcc
     6481 ttgagtctca aggcttccgc agacaattgg gtttcagcct tgatgacttc catatcgatt
     6541 tcttgacctt tttcaataaa tgccatctta ttctcctaac gttttgtaaa tttctctgac
     6601 aagagcttct gtgttatccc aacccaggca agggtctgtg atagacttac caaatacttc
     6661 tggctcattt tgtcgaccat cttccagata agactcaatc ataaaaccac gaacgaactg
     6721 cttaattttt tcattccaag cacggttaat caaggtctgg cggacaattc ggatctgttc
     6781 aatatactgc ttaccagaat tgtcatgatt ggtatcaatg atgataaaag gattttccaa
     6841 gcccattttc tcatactggg caatggtatc aattaaattg tcataatagt agttgggaat
     6901 attttttcca tactcattaa gagcaccacg aagaatagcg tgtgaaagcg ggttcccagt
     6961 tgtttctact tcttttccta agaaaaggaa actttgtttg ttttgagcag cataaatccc
     7021 attaaacatg acattgagat ttccagaggt tggattttta aaaccagtag aaaatcctgc
     7081 cccacttgcc acaaagcggt gttgctggtc ttcaactgaa cgggcaccaa ctgccatgta
     7141 agaaatcaaa tcatctacaa gcggaaggtt ttcaggataa agcatttcat cagcagttgt
     7201 catccctgtt tctgtgatga cacgatagtg aagatggcga acggctttga ttccattgat
     7261 aagactaggc gcttctgtcg cgttaggctg gtgaatcaag cccttatagc catctccgtt
     7321 ggtacggggt ttggcagtat aaacacgcat aaccataaag atacgatctg ccacttcttc
     7381 ttgtaggact gccaaacgct tagcgtattc aaggacagct tcttcgttgt cagatgagca
     7441 tggcccgatt accaagagaa ttcgctggtc ttctccacgt ataatggctt ctagctcttg
     7501 atctcgctgt gatttcctct ccaaagcctg accttctaat tttgacaagg cacgaacttc
     7561 ttctatattt attttagagc tttttgctgt aaataccata actcatctcc ttataaagca
     7621 aacggatacc cagtaaaaat ttacttttca caagatatcc gcctctaaac tatctcattt
     7681 tattgtcttt taccgtgaca gtttttaaat ttcttaccag aaccacatgg gcaaagttca
     7741 ttgcgtccaa tctggctcaa atccaaatct tctggcatac ttgcttggtg agcagcgata
     7801 ttgcgagtcg ctgttgtact gatatgacgt tctgcctgtg gtctttcttg ttcatgaatt
     7861 tgtgctttca tcatcaagcg tgtcacatca aactcaatcg aaccaatcat atcattaaac
     7921 atacggaaac cttctgcctg atactcaaca acagggttgt tctgagcata gccacgaagt
     7981 ccaaccgcgt tacgcaattg atcaagggca tcgatatgat ctgtccactt gttatccact
     8041 actcgtagaa tcaaaacttt ttggaattct ttaactgctt cttcatcgcg tagttttgaa
     8101 acctgactat cgtaaacctt caaggcacgt tggaaaagct cttccttgat ggccttatca
     8161 gacaagcctg acaagtcttc catcgtaata gaatcttcag gaagcaagtt gtacttagca
     8221 aagttcaaaa ttgcctctag tttttcatct tgtttggcac gcgcatgacc atcaacgaca
     8281 cgttcaatcg tgcgtttgat catagactga atttcaggtg ccaagtcacg atctgcagtg
     8341 atgacatcgt aacgttgagc atagataatc tcacgttgtt cacgcatgac atcatcgtat
     8401 tgaaggactt gtttacgggt atcgtagtta tttccttcga cacgtttctg agctgcttca
     8461 acctgacgcg tcaacatgcg agactcaatc gcctcttcag acatgttcaa gcgttcaaag
     8521 attcccttca agcgttcaga accaaaacgt ttcatcaaat catcttcaag agataggtag
     8581 aattgtgact cacctggatc tccttgacga cctgaacgtc cacgaagctg gttatcgata
     8641 cgacgacttt catgacgttc tgtaccaata acacaaagtc ctccaagttc acgaacacct
     8701 tcaccaagct tgatgtcggt accacgaccc gccatgttgg ttgcgatggt aacggcacca
     8761 cgttgaccag cattcatgat gatttgggct tctctatagt ggtttttggc attcaagact
     8821 tcgtgaggaa caccagctgc aaccaatttc ttagaaatgt agtcactagt ttcaaccgct
     8881 actgtaccaa ccaagacagg ttgacccttt tggtaacgag ccttaacgtc ttcgacaacc
     8941 gctttaaact tagattctat acttgcataa agaaggtctg agtggtcaat acgttgaaca
     9001 ggacggtttg ttgggattgg aataacacga atgttgtaga tttcacggaa ttcttcttcc
     9061 tcagtcttac ctgtacccgt cataccagac aatttcttgt acatacggaa aaggttttgg
     9121 tacgtgattg aggcagatgt cttggtttca tcctggattg gcacaccttc tttggcttca
     9181 atagcttggt gcaatccatc agaataacga cgaccttcca tggtacgacc tgtaaattgg
     9241 tcgacaatca agatttcttg ctcttcgctc accacatagt caatatcgag aagcatgatg
     9301 tagttggcac gaagggcgtt atcgataaag tgggtcaaag ccacgttttc gatgtcatag
     9361 aggttttcaa gcttgaagta gctttcagcc ctgtcaatcc ctgaatcaga caaaccaata
     9421 gtcttagact gcacatcgat gatgtagtca tctttgttca aagattttac atagtggtct
     9481 gccatgtgat acaactgact ggtttcaacc gcattggcac ctgatacaat caaaggtgta
     9541 cgagcctcgt caatcaagat agagtcaacc tcatcgacca aggcatagtt aagcggacgt
     9601 tgtaccatgt tttcagcgcg aacgaccatg ttgtcacgaa ggtagtcaaa tccgatttct
     9661 gagttagttg agtaagtaat atcacactca taggcttctt ttttctccat tggagatttg
     9721 gtagccaagt taatccctac tgacaaacca agccaagagt acaattcacc catctcagtc
     9781 gcgtcacgtt ctgacaggta ttcattaacc gtaactacgt gaaccccttt acctgaaagg
     9841 gcattgaggt ataccggcat ggtcgcagtc aaggttttcc cttcccctgt acgcatctct
     9901 ggcacgtcac catggtgaag aacaatcccc cccatgacct gaaccttata agggaagaga
     9961 cctaggacac gtttggcacc ttcacggaca accgcaaatg cttcgtaaag caatgaatcc
    10021 agtgattctc cattttgata acgttcctta aattcaactg tttttgcttt tagttggtcg
    10081 tcagtcaaag cagtcatttg gtcttcgtat ttgaaaacct tgtcagccat cttttccaga
    10141 cgacggattt ctcctttatc attttcgata attgttttta aaatattagc catgtttttc
    10201 cttactttaa attccgaata ttttagaatg ttcctttaat tttagcacaa ttactgatta
    10261 ttttcaagga agaatcccca ttttgaaaag gaaaaagagg ttagttttca agctaacctc
    10321 tccgactttt atgactgttt aattgccaaa aatcggcaaa atggcaagtg tgataaataa
    10381 gttaatccaa agagaaagac agcaaatcag tccaaaaaca aagagactga ctttcttgga
    10441 cagcaaggcc atgagaatgg acagaattcc aaaagctagc aagattttct gtatgatgaa
    10501 gatgtcaatc cttatcccca gaagtggttt atcccaaggg ataaagaaga aagacagcca
    10561 gatcagcaga actaaaccaa tataaacctt ggaatagcgt gtaataaatg attttagatg
    10621 tgccataagc catctcctag gattgtatat cctttacatt tgtatatgtc cattttcgac
    10681 aactaataca tctgtacaaa ggtctgaaat atcttccgat atgtgcgacg tcaggataac
    10741 cagaccattt tcttttttca ggtaagataa aatgacctgt ttggttaacc tcacactctt
    10801 ctcatccaaa gcattcatag gttcatcgag aaagagtata gaaggagagg aaataaaggc
    10861 ttgaatcaag gccatttttt gctttgttcc taaggataaa tgacggtatt caatgtcttc
    10921 aaattcctgt aaatcatagt attgaatcca ataggcaatt cttttttccg taactttaga
    10981 tgaaaaatac cttaacagtt ccaaattttc tctcagggat aaatgagata aaaactcgac
    11041 tttttcaatt aaaattcctg catcctgaat ataatgattt tttaccccgt aaactttacc
    11101 atcttgaaga acttttcctt tgtcaagctt aatataacca gcaagtatct ttaaaagaac
    11161 cgtcttgcca gatccattat cacctttaag tccataaatt tttcctgatt gaaacaccaa
    11221 attcaactga tttaaaatag gtcttttccc ataaattttc tgtccattta taatttcaag
    11281 tctcatcgtt tatcctttct taaatttctc ttccaagcga ctaacaatca acataactaa
    11341 taaacttaag agtagtaaat acactgttac aatatgatcc atcatccatt cttgtcccac
    11401 aaatgctaaa aaatagagaa aaaaggaaaa accagcgccc gcactaatca gtgctatcag
    11461 ataagacagt agtaagtaca acatcattaa ctgagcaagt aaaaagaaaa gtgcgactgt
    11521 tttgaaatga attcctagag gaagtaaacc aagaaaccag acaaacaaat cttgagtaaa
    11581 aaatttagaa atgacaacca atcgtttcct tgtataatcg aaaaaggagc ttgattgata
    11641 gcgaatcaag tgatggtaat ggtctgccaa atcactttca agtaaaatga cagagagcca
    11701 tcctaccatg aaaagcgaaa ggtttaacaa gagtttgggg atttctaaat tcacttcaga
    11761 atagtcatta taggaaaaaa ttgaaatcaa ttctctcttg aaaaagataa cagcatcctg
    11821 cttttgaacc gctgttgaca atagatggtt tatcaaaatc atcatcagaa ctatacgaag
    11881 tagtaaaaca atctttctct ttagcaaaat attgttaaaa ttcttaaaca ttatcttcat
    11941 atttttcaag caccatgtaa gagacaatat aaaggatata aggagtaaag agtttcatca
    12001 aacttacatc cccatagcta gcttgcatca aactcgtcat aggaaccata tagtaaggca
    12061 aggcactata gagcagcata gaaccaagcc aaagaaacat caaataggtg attgccgaac
    12121 gagtcacatt tcctaaataa tcaactattt gtaaaaaata gactgcattg atgaaaatac
    12181 cgattagtag gacacaagta aagaacaaat agcttgttat ctctccatct aggagccttt
    12241 gtaaactact cccatcagaa aatagagaat tccatccaag aggagaaaaa gtcccaaaga
    12301 aatgagttaa aattgcaatt atcagcacag tcactaaata tatcaaacaa gggatacttg
    12361 caacttgcaa agtcaacttt ctttttaacc cttgataact cacttctctt ccaatactca
    12421 atcgtaaaac tttatttttc agctcaatat attgaaaacc tagcaaaaca atgatgattg
    12481 gaataataaa tgattgataa aaacctagat tgaactggag aatacgaaaa tctaatgctg
    12541 attttaataa gtcattttta attgcttgtc catcgaattt agctagatcg ggcgcatgaa
    12601 tatcaaaaaa gaaataagtc atcaaaacat cacgataaac caaagcaaga aaaagcaaag
    12661 taagcaagac ataaacaaat ttatgccttg ccaaagttaa gagaggaatc ttcataagcc
    12721 acctcctaca aatagaaagt aatataatac tcaatgaaaa tcaaagagca aactaggaaa
    12781 ctagccgcaa gttgctcaaa gcactgcttt gaggttgtag ataagactga cgaagtcagt
    12841 tacatatata atccaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtataaat
    12901 caatgccttc cgcctttaga cttccctagt tcccgtctca agtcaagcat ttttttgaaa
    12961 cagatatagt ctaaccagtc actagcaaaa gcgataagta gtaataatag ccaaataccc
    13021 cataacttga tatctgtcaa atttctcaag acgatattga aaaacagaac tgaaaaaact
    13081 gtccaagcaa ggctaaaaag agcatagagg gtgatgtaaa tccagtaaaa atagtaaaaa
    13141 attgggaaaa atttactttc tctattatcc ttgtcaatcc agctgaaaaa gtaaaaacag
    13201 ggtaataaga ttatcatttt aaacaagtgt tccatcacaa acctcctctt ttaaattatt
    13261 ttctttagtt tcgttctatt aaatgttatt accaatataa accttggaat agcgtgtaat
    13321 aaatgatttt agaggtacca taagccacct cctacaaata gaaaccgata taaatcaatg
    13381 ccttccaccc ttagacttcc ctagttcctg tctcaagcga aacatttctt tgaaacagga
    13441 ataagttaac caattcatac caatagctag cagaataaaa agaaaccaaa tgccccataa
    13501 cttgatatct gtcacatttc tcaagacggt attgaaaaac agaactgaaa caactgtcca
    13561 agcaaggcta aaaagagaat agaaggggat gtaaaaccag taaaaataat aaaaaattgg
    13621 aaaaaactta ctatttctgt tggccttttc aatccagtta tcaaaataaa agtacggtgc
    13681 taaaagtaag aatttaaaca aatgttccat caccgacatc cccccttctt ttgatagcgt
    13741 tttctattat tttattatat caaaaaaatc cggaactgtc attccagatt ctactttttt
    13801 atttgcgttt tcttgcgatg agatgaatcg gtgttccctc aaaaacaaag gccttgcgga
    13861 tttgattttc caagaaacgc aggtaagaaa agtgcatgag ttcttcttca ttgacaaaga
    13921 tgacaaaggt tggtggtttg gttgccactt gggtcgcata gaaaatcttg agacgttttc
    13981 ctttgtctgt cggtgttggg ttgatggcaa tggcatccat gatgacatcg ttcaagacag
    14041 ctgatggaat acgtgtattt tgactttcgc tgatttgctt aatcatctca ggaagtttgt
    14101 ggagacgttg cttggttaaa gctgatacaa agataatcgg tgcgtaaggc aggtattgga
    14161 actgctcacg gatatcttct tcccagtttt tcatagtgtg gttatctttt tcaagcgtat
    14221 cccacttgtt gaccacgata atcatccctt taccagcttc atgggcaaat cctgcgatac
    14281 gcttgtcgta ctcacgaatg ccttcttccg cattgatgac catcaagacc acatctgaac
    14341 ggtcaatagc acgcatggca cgcataacag agtatttctc agtattttca taaaccttac
    14401 cagacttacg cataccagcc gtatcaatca tggtaaactc ttgaccatct gtatctgtaa
    14461 agtgggtatc aatggcatca cgagttgttc cagcaacagg actagcaata acacggtctt
    14521 ctcccaagat agcattgatc aagcttgatt ttccaacgtt aggacgacca atcaagctaa
    14581 acttaatgac atctggattt tcttcctcat attcatttgg aagattttct acgatcgcat
    14641 ctagcacatc ccctgtaccg attccatgga cagatgagat aggcaatggt tcacccaaac
    14701 cgagagcata gaaatcatag atatcatttc tcatttcagg gttgtccacc ttgttgactg
    14761 cgaggataac tggtttgtgg gtcttataaa gcttacgagc tacgtattcg tctgcatcag
    14821 taattccttc cttaccagac acgacaaaaa cgataacatc tgcttcttcc atggcaattt
    14881 ctgcctggtg cttgatttgt tccatgaaag gagcatctac atcatcaatt cctcctgtat
    14941 caatcatact aaaagagcga ttgagccact cacccgttgc atagatacgg tcacgtgtca
    15001 ctccttcgac atcttctaca atggagattc gctcaccagc gatccgatta aatagggttg
    15061 atttcccaac attgggacgt cctacaatgg caatagttgg tagggccata atttctcact
    15121 ttctacaata atttcttctg ttcaagattt tttctagttg agcttggttc tgcttggcca
    15181 aactgttctg ctagacgctg actccagctt gtggtcgcac gcgctccagc atagtcagcc
    15241 tgcacacggt cataagcttg gattgcctca gttgactgtt cttggtattc ttcctcaaag
    15301 actacattat ctagtggcag tctcggtttc acatcatgag tttgatttgg aacacctagt
    15361 gccatcccaa agacaggata ggtgtagtca ggcaggttaa agagttctgc cacttcttct
    15421 gacttgtacc gtaccaagcc aataatcaca ccaccataac ccaaactttc agctgccagc
    15481 agggcatttt gtccagccaa agccgcatcc accgaactaa tcaagagacc ttccacacct
    15541 tggggttgga aggtgtcggt atgaagtcgg gctccctttt ctgctcggtt caaatctccg
    15601 acaaagagaa ggaaaacagc agactggcga atggcttctt gaggtaccaa ttcatacaag
    15661 gcatctttct tctcttgact tcgtaccaca atcacagagt aggattggaa attcttccaa
    15721 gatgatgcca tctgggctgc tgtcaaaatc tcatttaagt ctacttgggg aatttcttgc
    15781 tctttaaacc tgcgcactga agtatgagcc ttcatcaatt taatggtttc tgtcatcgac
    15841 ggtttactcc ttctaaacga gtctcctcag ccaaataacg gatgcgttcc atgacccgtc
    15901 tggcttccca ggtttcgtca tttccatgtt tcactttcgc aaaatgcttc tccaaatctt
    15961 caaagttgaa gttggatgtg aaaaaggtcg gtaaattttc ctgcatccga tattggagaa
    16021 tgacctgcag gatttcgtca cgcacccaaa cggttgattg ctcggcgcca atatcatcta
    16081 aaatcaggac ctcagacagc ttaatctcat ccaccaaggt cttaacattg ccatcactga
    16141 tagcattttt gacatcaatg acaaagctag gatagtggag gagagttgat gaaacaccac
    16201 gtttttctga taaatcatga gctaaggccg ccaccatgaa acttttaccc acaccaaagt
    16261 ctccatataa gtaaagacct tttcgaatag ctggatattg ctccacgaag gctagtagct
    16321 tttcaaaaac tggtaagcgc cccaaatcat ccaagtcaac ttgagccaaa ctagctttct
    16381 tgagactggc tggtagattg attaacttga gacggttctt aatagccgct tctttttcag
    16441 ccgcgattag ctcaggagtt tcttcatatg aaacatctgc atagccatga ttcttaacca
    16501 aaatcggctt gtagcctttg gcaatataat ccgtatcccc acggagaaac ttgtcacgct
    16561 cggtgatgta ctgattaaac ttggagatac tgcgatttaa ttcctctgga gtcaaggatt
    16621 cttgctggat aaaagccgca acatcagggt ccttcatgat tttctggacc aaatcttgat
    16681 aataaaaacg gctgggttga cgtttgagta cgtctccgac actttccatc taatctcctc
    16741 ctttttctaa tcgagctaat agttcttgct tcttacgttc tagttccaga cgagtttcct
    16801 cgctggtttc attcttatat tcaggattac tccatttagg aacattggtt tttactgggg
    16861 cagtctgatt ctgtttttgt atttttgctt tctgtcctcg ctcacggatg cgcaagactg
    16921 ccttttctgc cgaatgaatc ttttgatagg catagtcatt ggctaccttc atggcatatt
    16981 tctcattgat atttgccgaa tcgaccttat taaaggtcaa taaaagaatg atattgatga
    17041 cttcgtccag caagcccaag ccagccatct gttgcaagag ttctctttct gtttgggtaa
    17101 tggttccctt gcgtgtttgc ttgatttctg ccaagaactg cagggcagtt ttacttttgg
    17161 cttctttgat aatggttgtt tccttaagac taaagtcaga ggaaactggt ttttgagcaa
    17221 ttttttcccg catgcgtttg gttgaaataa cctgagaaac agctgttgac ttggccaatt
    17281 gataggtttc aaaccaagtc catttcttct cctcggcaat agcaaagagg tttaagacat
    17341 cggactgctc atccgcaaaa cgaagtccat ctcgagccat cagctggcga aaatggtcca
    17401 agtcaaaatc gttggccact ttcttcttga gaccaaggtc ttcttgactg cctagttctg
    17461 ccaattctgg aaagacttga ttgagtgaga caggtatttc ttcaccatca gcactttcaa
    17521 ctttcaaatc ctccacagct acatcgccaa tctttttctc taagagtctg cgataaacag
    17581 gatgctccaa gaagtcttga ctagatagag gagcatggag gactagctga taaacatccc
    17641 ccttttgata gagggtcaag agattaaaag cagataagat tttcaatgat tttatcagtc
    17701 tatccatccc aaagttaaga tggttgagaa tgcttgaaaa aagatattcc tttctaccat
    17761 tatcccaaaa actgattgta taaagataaa ggctcagtgc ctcctgaccg ataatcggga
    17821 ggtagcactg taccagagat gaggtatctt gcgacacccg attattcttt agataagaaa
    17881 aacggtcaat tggcttcatt tatctttcct ttttcttttt agaggactgg gtgatttgtt
    17941 ggagcaagct ctctaactca ctgacatcct taaaactacg atagacacta gcaaaacgta
    18001 cataggtaat ctcgtccaat tcagccaact cctccatgac gagtgaacca atgtcctcac
    18061 tttgaatttc attttcattt cgaccacgga gtttctgttc gatacgattg actaccatgt
    18121 tgatttcatc acttgacaca ggacgtttct gggctgagcg gataatccca ttaaagattt
    18181 tatctctgga gaattgttcc cgtgtgccat cttttttaac aaccactaag gttctttctt
    18241 ctactcgttc gtaggttgta aaacggtgtt ggcattcgtc gcactcacgt cttctacgaa
    18301 tggtgttccc ttcttctgct tggcgactat cgataacact tgacttggta gccccacatt
    18361 ttggacaacg catcctttcc ctccttatcg ttttcttttc attataccat tttttaagcg
    18421 attcccaaaa caattcttct ttttgcttga caagtttttt gttttgttgt attatttaat
    18481 taagacaaaa gggtaaaaga aaggagacta agatgtcctg gacatttgac aacaaaaaac
    18541 ccatctattt acagattatg gagaaaatca agcttcagat tgtttcccat acactggaac
    18601 ccaatcaaca acttccaacc gtgagggagc tagctagcga ggctggtgtc aatcccaata
    18661 ccatccaaag agccttatca gaccttgaac gagaaggatt tgtctacagc aagcgaacaa
    18721 ctggacgatt tgtgactaag gataaggagc taatcgccca gtcacgcaaa caattatcag
    18781 aagaagaatt ggaacacttc gtttcctcca tgacccattt tggctatgaa aaagaagaac
    18841 taccaggcgt agtcagtgat tatattaaag gagtttaagc ctatgtcatt actagcattt
    18901 gaaaatgtat ccaaatcata tggagcaaca ccagcccttg aaaatgtttc tcttgacatt
    18961 ccagctggaa aaattgtcgg ccttcttggg ccaaacggct caggaaaaac aaccctgatt
    19021 aaactaatta atggcctctt acaaccagat caaggacgtg tcctcatcaa cgacatggac
    19081 ccaagcccag caaccaaggc cgttgtagct tatttgcctg atacgaccta tctcaatgag
    19141 caaatgaagg tcaaagaagc cctaacctac ttcaagacct tctataaaga tttcaatctt
    19201 gaacgcgctc atcatctact tgcagacctg ggcattgatg aaaatagtcg tctcaagaaa
    19261 ctatcaaaag gaaacaaaga aaaggttcaa ctgattttgg ttatgagccg tgatgctcgt
    19321 ctctatgttt tggatgaacc cattggtggg gtggatccag cagcccgtgc ttatatcctc
    19381 aataccatta tcaacaacta ctcaccaact tctaccgttt tgatttctac ccacttgatt
    19441 tctgatatcg agccaatctt ggatgaaatt gtcttcctaa aagacggaaa agtcgtccgt
    19501 caaggaaatg tagatgatat tcgctacgag tcaggtgaat ccattgacca actcttccgt
    19561 caggaattta aggcctaagc aaaggagatt atttatgttt tggaatttag ttcactacga
    19621 atttaaaaat gttaacaagt ggtatttagc cctctacgca gccgtgctag tcctttctgc
    19681 cctcatcgga atacagacac aaggctttaa aaatctacct taccaagaaa gtcaggctac
    19741 tatgctactt tttctagcta cagtctttgg tggcttgatg cttacacttg cgatttcaac
    19801 cattttcttg attattaaac gcttcaaagg tagtgtctac gaccgacaag gctatctgac
    19861 tttgaccttg ccagtttctg aacaccatat catcacagcc aaactaatcg gtgcctttat
    19921 ctggtcattg attagcaccg ctgtattggc tctaagtgct gttattattc tggctttaac
    19981 agctccagaa tggattcctc tttcttatgt gattacattt gtagaaacac atctccctca
    20041 gatctttctt acaggtatat ccttcctact aaataccatt tcaggaatcc tctgcatcta
    20101 cctggctatt tccattggac agcttttcaa tgaataccgt acagcactcg ctgttgcagc
    20161 ctacattggt atccaaatcg tcattggatt tattgaactt ttcttcaatc ttagttccaa
    20221 tttctatgtc aattcactgg taggactcaa tgaccatttc tatatgggag caggtatagc
    20281 cattgttaaa gaactcatat tcatagctat cttttatctc ggaacctact acatcttgag
    20341 aaataaggtt aatttgcttt aaataatttt tacctagata tgtaacatac tcatagaaca
    20401 aaagagacca ggcaaaaagt ctttaaaatt agaaaacgca tagtatcaag tgttaaaaat
    20461 ccttgatatt atgcgttttt tgtgagctga cttatttcct ttcgctatat cgcaaaatga
    20521 aataagaacg gaacgatggg attttggaat tcaaatcaat ttataagaat gttttagaag
    20581 taatattatc ctattccaga ttcagttcac tatacaattg agttttcaag caacctgttt
    20641 acataatgtg tacataatta ggttcgtgat tccacccttt tcacctttaa aaacctcgct
    20701 ttcgcaaggc tcttctattt ataagataag gcacgtttaa aggttttcca aatccctaaa
    20761 tcatccgttt gaagaacgag actagcatac atgcgtccga taaatcctgt tgctaccacc
    20821 gcaaaaatca ctgtaatagc aagtgaaatc catgcttccg ctccctccgc atagccatta
    20881 atcgttcgaa acggcataaa gaaggtcgaa ataaagggaa tataagaacc aatcttcaag
    20941 aggagattgt caccagctgc acctagagct gtcactccaa aaaaaccacc cataatcaaa
    21001 atcatcaaag gcgacaaggc tttccctgag tcctcaggac gagaaaccat agatcctagg
    21061 aaggctgcca agactacgta catgaaaaga ctgatcaaaa taaagagcag ggtattcagt
    21121 gagatagcat ctcccaagtg aaccaaaata ccagactgag ccaagaatgg caaatcttta
    21181 aagagcaaaa cggcagccag accacctaca acatagatcc caatatgcgt taaaatcact
    21241 agaaacagag ccatcatccg cgcatagaaa tagtgacttg cccttatgct agaaaaaacg
    21301 acttccataa ttttggtgcc tttttcactg gcaacttcct gagctgttac acccgcatag
    21361 gtaatcagaa tcatataaag aaagaatcct aaggcacctg ctgcaattgt ttgaataaac
    21421 tttttatttt ccttggcttc atcaatcttt tctgtgaatt gaattgtctg cgctaagcgt
    21481 ttttcctgct cttgagacaa ggaagcagtt gaacgattaa gctgattttg cagttcattg
    21541 agtgtacctg taacctcaaa tttaattcca ttttcaagcg atgtttcgcc atgataaact
    21601 gcctttagaa cactatcttc ttgatcaatg gtcaaataac cttttaattt ttcttcttta
    21661 attgcttctt tggcacttgc ttcatcttta tagtcgaagt taacaccatt tacattcttc
    21721 agtccttctg ctacagatgg cactgttgtc actactgcca ctttattatt tttagccata
    21781 gaagaacctt ggagatgccc aattcctaca gagattccta aaaagaggaa cggcgaaatc
    21841 accataaaga agaaactcca tgactcgaca tgtcgaagat aggtttcctt gattacaacc
    21901 cacatatttc tcatacttcc actcctgatt ctagtttaaa gatttcatcg atagttggcg
    21961 cttgttggtc aaatgttgcg atatattgac cttgagtcaa gattgggaag agttcccttc
    22021 cagcgctctc atcctccaaa atcaatttcc aactgccttg tttggtcaag ctcacctgtt
    22081 tgacatgagg aagattttcc aattcttcct tgcttcgttc acttgaaaca aagagacgtg
    22141 ttttcccgta ttgattgcgg acatcctgaa ctggtccgtg caagaccaca cggccatctc
    22201 ggatcatcag aatatcgtca caaagttcct caacattggt catgacatgg tcagaaaaga
    22261 taatggttgc tccgcgctct ttttcctgaa aaatgacttg tttgagcaat tctgtattaa
    22321 ctgggtccaa tccactaaaa ggctcatcca agataatcag gtctggttca tgaatcagag
    22381 taataatgag ctgaatcttc tgctgatttc cttttgacaa actcttgatt ttatctgcca
    22441 gctttccttt cacttccaac ctcttcatcc attgagggag tttttctttg acttctttgg
    22501 catccatgcc ttttagagtc gccaagtagc gaacttgttc aagaactgtc aatttaggca
    22561 tgagactgcg ttcttcaggc agataaccaa tccgagcata ggtctcctga cgaatatcct
    22621 gaccatccag accgatttct ccctgatatt ctaggaattt taaaatacta tggaaaatcg
    22681 ttgtttttcc agcaccattt tttccgacta gtcccaaaat acgacctggt cgcgcttgaa
    22741 agtcaatacc aaacaaaact tgcttggatc caaaactttt ctctagactt cttacttcta
    22801 gcatctttca cctccgaaat ttcttgcact cattatactc ctttttgata gcctttacaa
    22861 tgttttttgt tcatttttag aagactattg ctgtgtaaaa tatggcctgg agcactttta
    22921 tactcaatga aaatcaaaga gcaaactagg aagctagccg tagactgctc aaagtacagc
    22981 tttgaggttg cagataaaac tgacgaagtc agctcaaaac actgttttga ggttgtggat
    23041 agaactgacg aagtcagtaa ctatatctac ggcaaggcga agctgacgtg gtttgaagag
    23101 attttcgaag agtattagtg ataaatccat tatacagcag caaacttaat ttataccttc
    23161 cgctcctcaa ctgtctattt ttaatcctga attgttattt gagtaactcc tttttcctca
    23221 taaagttttc ttcctctaaa acttctggaa aaaagcgaat agtttcagac aacattttta
    23281 taagaaacaa gttcatctgt catttcaaga aggagtaatc ctttatctac taatggacgg
    23341 aacggaattc aaccgcttgt cccatatgtt ttctaaggat tatatagcaa aatgaaataa
    23401 aaacaggaca aatagatcgg gacagtcaaa tcgatttcta acaatgtttt agaagtagat
    23461 gcgtactatt ctagtttcaa tctattatat ctattatgca caccactata ggatctaata
    23521 aaaatcacaa caagttcatt catagatggt tacctcaggg aactaagaaa acgactacaa
    23581 aggaagtcgc attcatcgaa aagtggatta acaactatcc taaaaaatgc ttgaactaca
    23641 agtcccccag agaagacttc tggatgacta acttgaactt gaaatttagc aataattaat
    23701 tcaccatcta actatattta gtaattattt cagaactgat taatattaaa attaactaac
    23761 aattcaaagg attcatacta gccataaatt acgtccatca gagagagact cttactactt
    23821 ttagatttta gtctttctag cttcagaata catctaaact ttagggaaaa tgactattcg
    23881 aaagcgcgaa tgcctcaaaa ttatctcaga taagctattc gaaacttaga atgcttttaa
    23941 attcatggaa ttgcgattat tcgaaaccta gaatgcatat aacctttagt tgacagacct
    24001 attctaagtc tcgaagggct atttactttc tattccttat caaaaaagac tcattccccc
    24061 tttctcctcc aaaatatggt atagtagaaa tatactatct atgaggagtt tacatgtcac
    24121 aggataaaca aatgaaagct gtttctcccc ttctgcagcg agttatcaat atctcatcga
    24181 ttgtcggtgg ggttgggagt ttgattttct gtatttgggc ttatcaggct gggattttac
    24241 aatccaagga aaccctctct gcctttatcc agcaggcagg catctggggt ccacctctct
    24301 ttatcttttt acagatttta cagactgtcg tccctatcat tccaggggcc ttgacctcgg
    24361 tggctggggt ctttatctac gggcacatca tcgggactat ctacaactat atcggcatcg
    24421 tgattggctg tgccattatc ttttatctag tgcgcctata cggagctgcc tttgtccagt
    24481 ctgtcgtcag caagcgcacc tacgacaagt acatcggctg gctagataag ggcaatcgtt
    24541 ttgaccgctt ctttattttt atgatgattt ggcccattag cccagctgac tttctctgta
    24601 tgctggctgc cctgaccaag atgagtttca agcgctacat gaccatcatc attctgacca
    24661 aaccctttac cctcgtggtt tatacctacg gtttgaccta tattattgac tttttctggc
    24721 aaatgctttg acacgtaaaa aatccgtttg gtttcccaag tggattttta aagcgtagat
    24781 taactatagc ttgatactaa atatactttg gtatggaaat catgcatatt tttcgatagt
    24841 gaggcgagga cttacctagc ctttccgccg tgatagaaac acctgaaatc taatggtttc
    24901 aggtattcgg aaactttgag cctagtgtct caaagtttag gtatggaatt tcgaagaaag
    24961 tcgctaccgt ccgtaatcac ttaaggaaag gctcaaaaat attgttttca accacaaaat
    25021 ccgtttggtt tcccaagcgg attttgtgct ttattttgaa acttcttttg caagaacaaa
    25081 gttcccaagt gtggcagaac catttcctgc gactgctggc gtcacgatat agtcacgcac
    25141 atctggtact ggtaggtaac cattaagaag agatgtaaat ttctcacgga cacggtccag
    25201 catatgttgt tgagccatga cccctccacc aaagacaatc acgtctgggc ggaaagtcac
    25261 tgtcgcatta accgcagctt gagcgatata gtaggcttga acatcccaaa cagggttgtt
    25321 gagttcaata gtttccccac gtacacctgt acgagcttcc aaacttggac cagctgcata
    25381 accttctaga catcccttat ggaaaggaca aacaccctta aactcttttt caatatccat
    25441 tgggtgtcta gcaacataat aatgacccat ttcagggtga cccacaccac cgataaactc
    25501 accacgttgg atgacgcccg caccgatacc tgtaccgatt gtgtagtaaa ccaagttttc
    25561 gatacgacca ccagcattgt tacgggcaac cacttcaccg taagcagagc tgtttacgtc
    25621 tgttgtgaag tacattggca cgtttagggc gcgacgaagg gcaccaagca agtccacatt
    25681 tgcccagttt ggttttggag tcgtcgtgat aaagccataa gtttttgagt ttttgtcaat
    25741 atcaatcggc ccaaatgaac caactgcaag accagcaagg ttatcgaatt ttgagaagaa
    25801 ctcaatggtt ttatcgattg tttcgattgg agttgttgtt ggaaattgtg ttttttctac
    25861 aacgttaaag ttttcatcac cgacagcaca gacaaacttt gtaccgcccg cttccaagct
    25921 tccatataat tttgtcatga taaacctctt gtttttattt tctttattat agcatacttc
    25981 gaaagtctaa atgtctctat tttttagatt ttcctctgta aatcttactc tctaataaaa
    26041 acgaacaaac atgtcatttg ttcgttttta ttagagagga ttgattagat tttcacttcg
    26101 atcacagcat cccccttcgc aactgaacct gttgcgactg gagctactga agcgtagtca
    26161 gctgtatttg taacgataac cattgttgta tcatcaagtc cagctgcagc gattttgttt
    26221 gagtcaaatg ttccaagaac atcgccagct ttcaccttat taccttgagc aacttttgct
    26281 tcaaaaccgt caccgttcat agatacagtg tcaataccaa cgtgaatcaa aacttcagca
    26341 ccatttcttg ttttcaaacc aaaagcgtgc cctgttggaa aggcaattga aacttcagca
    26401 tcagctggtg catagaccac gccttggctt ggtttcacag cgataccttg tcccatagct
    26461 ccacttgaga agactgggtc attgacatca gcaagagcga caacatcacc aacgatagga
    26521 gttacaagtg tttcattttg aagagctgct ggcgcaactt cttctttttc ttcagccact
    26581 tcagcttgtt ttgcagctgc agttgcgtct acttcatctt cgtaaccaaa catgtaagta
    26641 agagcaaaac caagggcaaa tgatacagct accataagaa ggtattgtgg aagttgtccg
    26701 ttaccaacat aaagcattgt accagggatg atggtgatac cattaccagt accagcaagt
    26761 ccaaggatag aagccaatcc accaccgatt gcaccagcaa tcaatgaaag gaagaatggt
    26821 ttacggaagc gcaagttcac cccgaagata gcaggctctg taatacctag gaaggcagaa
    26881 agagcagccg ggaaagcaag tgttttcagt tttggatttt ttgttttaac accaaccgca
    26941 acagtagcag caccttgagc tgtcatagca gctgtgatga tagcgttgaa tgggttagca
    27001 tggtcagcag caagtaattg cacttcaagc aagttgaaga tgtggtgcac acctgacacg
    27061 acgatcaatt ggtgaacccc accaatcaag aaaccaccaa gaccaaatgg aatgctaaga
    27121 atcgcttttg tagcaataag gatgtagttt tcaacaacgt ggaaaactgg tccaatgaca
    27181 aagagtccaa ggatagacat gaccaaaagt gtcacgaatg gtgttaccaa gaggtcaatg
    27241 acatctggaa caaccttgcg gacagctttt tcaaatttag ctccgacaac cccgatgatg
    27301 aaggctggaa gaacggaacc ttgcaaacca acaacaggga tgaaaccaaa gaagttcatc
    27361 gctgttactt caccaccttg agcaactgcc caagcgtttg gaagtgagcc agagacaagc
    27421 atcataccaa gaacgatacc aacggcagga tttccaccaa atacacggaa ggttgaccac
    27481 acaaccaaac ctggcaagat gatgaaggct gtatctgtca agatttgtgt gtaagttgca
    27541 aagtcacctg gaagtggcat ttcaagagcg ttgaaaagac cacgcacacc catgaagaga
    27601 cctgtcgcta cgataactgg gatgattgga acgaaaacat caccgaaagt acggatagca
    27661 cgttggaacc agttcccttg tttagcaact tctgctttca tgtcatcctt agatgatgtt
    27721 ggtaagccaa gtacaacaac ttcatcgtac attttgttaa ctgtacctgt accaaagata
    27781 atttggtatt gccctgagtt aaagaaagca ccttgaactt tttccaagtt ctcaatcact
    27841 tctttattga ttttctcttc atctttgacc atgacacgta gacgagtcgc acagtgggca
    27901 acactattga cattttcacg tccgcccaag gcatcgatga ctttttttgc aatttcctga
    27961 ttgttcattt gcaaaaatct ccttatataa cattttgttc ttgtttgaaa gcgattttat
    28021 tcgcccttac gactattatt ttatcatgtt ttaaaaatat gtcaagcgtt ttgcagaaaa
    28081 aatattctat ctacttagtg ggcaagcttt agattgattg tttttgactg tttcatcagg
    28141 aatttcttga aaaataagtt tgaaaccttg gttaaaaagt ctt
//
LOCUS       DAVKGC010000012        28088 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKGC010000012 DAVKGC010000000
VERSION     DAVKGC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.012"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..2279
                     /locus_tag="VW049_000453"
     CDS             <1..2279
                     /locus_tag="VW049_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9431629.1"
                     /translation="KTISTIRDFENKDLKKLIKKKYKQEDDFVNGGTRTVERDYKYDD
                     KGNIIAYDDGTDLEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGN
                     NYKSIEIKATKYDFHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDNDQKKAEIKIRM
                     PEKIKETKSEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDN
                     IILRKGYALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNV
                     EDGRSTQAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKE
                     YTGEDKSNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISD
                     KGGFNWELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPD
                     KVTDMDGNVYLQTGYSNLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVF
                     NINDKRNNGFDGEIQEQHIYINGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEF
                     ILNKDTGEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEIS
                     GFEGKKDAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNES
                     HRKEDLQREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAA
                     GIMFIVGIFLGLKKKNQD"
     gene            2906..3382
                     /locus_tag="VW049_000454"
     CDS             2906..3382
                     /locus_tag="VW049_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9431630.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTENGHLEDLSKISEPEALYAYIAEATA"
     gene            3420..3725
                     /locus_tag="VW049_000455"
     CDS             3420..3725
                     /locus_tag="VW049_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9431631.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            3773..5248
                     /locus_tag="VW049_000456"
     CDS             3773..5248
                     /locus_tag="VW049_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9431632.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            5271..5348
                     /locus_tag="VW049_000457"
                     /pseudo
     CDS             5271..5348
                     /locus_tag="VW049_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            5598..12299
                     /locus_tag="VW049_000458"
     CDS             5598..12299
                     /locus_tag="VW049_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9431633.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSL
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTNLNSVDKSVSYVLTD
                     GSVQEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQIFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAKLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDSYETKDLTVEWEEVPAEN
                     LTKAGQFTIRGRVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(12547..13403)
                     /locus_tag="VW049_000459"
                     /pseudo
     CDS             complement(12547..13403)
                     /locus_tag="VW049_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13613..13972)
                     /locus_tag="VW049_000460"
     CDS             complement(13613..13972)
                     /locus_tag="VW049_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9431634.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            14064..15020
                     /locus_tag="VW049_000461"
                     /pseudo
     CDS             14064..15020
                     /locus_tag="VW049_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            15004..15561
                     /locus_tag="VW049_000462"
     CDS             15004..15561
                     /locus_tag="VW049_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9431635.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            15554..15808
                     /locus_tag="VW049_000463"
     CDS             15554..15808
                     /locus_tag="VW049_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431636.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            15820..17874
                     /locus_tag="VW049_000464"
     CDS             15820..17874
                     /locus_tag="VW049_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9431637.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            18053..18913
                     /locus_tag="VW049_000465"
     CDS             18053..18913
                     /locus_tag="VW049_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9431638.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            19124..19834
                     /gene="ccdA2"
                     /locus_tag="VW049_000466"
     CDS             19124..19834
                     /gene="ccdA2"
                     /locus_tag="VW049_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9431639.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            19854..20420
                     /locus_tag="VW049_000467"
     CDS             19854..20420
                     /locus_tag="VW049_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9431640.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            20431..21543
                     /gene="msrB"
                     /locus_tag="VW049_000468"
     CDS             20431..21543
                     /gene="msrB"
                     /locus_tag="VW049_000468"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9431641.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            21766..22503
                     /locus_tag="VW049_000469"
     CDS             21766..22503
                     /locus_tag="VW049_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9431642.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            22500..24191
                     /locus_tag="VW049_000470"
     CDS             22500..24191
                     /locus_tag="VW049_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9431643.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFEANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            24307..24870
                     /locus_tag="VW049_000471"
     CDS             24307..24870
                     /locus_tag="VW049_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9431644.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            24892..>28088
                     /locus_tag="VW049_000472"
     CDS             24892..>28088
                     /locus_tag="VW049_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9431645.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSVESAPVEEVGGAVDSKTDEQAPVKSESQPSDKPAEES
                     KVEPSVEPVAPREDEKAPTAPVEPEKQPEVPEETVEPKEETKTAKGTQEKGKEGQAPV
                     QEVHPEYKVTTGTVEKSIESELDFTTDVVPDDTKYVGDEVVENPGSKGVQVTKTTYET
                     VEGVETDKVLSTSTEVTKEPVNQQVIRGTKPIEGTLIEESLEKIPFKEVVKEDDQLKK
                     GLEVVAQEGKEGQKKITKTFNTIKGVKTEDAPKVTEEILEEPQDKILKRGTKSFEKPV
                     LTITEIESKDLKRTSDVKYSLENPSKAAIKSITLTLKKGDEIVKTLNVSPEDLTTTLT
                     DLQYYKDYKLETKMVYDRGEGDEEEVLKEEPLRIDLKKVEIKDIIRTDLIKYENQVET
                     DETRLTSVPTDLSNYYLKVTSNDHKTNLLAVQKIEESTIDGKQVYKVIAVADNLVQRN
                     VDNRFEETYTYYIEKPKASQAGIFYDFEELIQAIQTNPAGEFKLGQSMSARNVVPKGK
                     SYITSEFTGKLLSDGDKRYAIHNLEHPLFDKIKAGTVKNINFENIDIDLSNQDNIAAI
                     ANEISGTTRIENIKAIGTITARNNVAGFVNNIKEDATLENVAFIGSINSAGNNSTVGG
                     IAGSNYMGFVNRAYVDATITANNANASMLVPFVQYKAHSWLSGTKGKLTNSVAKGILD
                     VKNTKYVGGITSKTWPYGAVQDNVTYAKVIKGQEIFGSNDVNDGDANPFVSNLFGVIG
                     YSSSEDGTGKDNKNKNKLKHLSKSEADAKVASFNITAAKFVSEPYELNKLNNVSSREA
                     AYESIQDYNADYKLAYKNIEKLQPFYNKDFIVNQA"
BASE COUNT         8944 a         5061 c         6091 g         7992 t
ORIGIN      
        1 ataaaactat ttcaacaatt agagactttg aaaataaaga cctaaagaaa ctcattaaaa
       61 agaaatataa gcaagaagac gactttgtaa atggtggaac aagaacagta gagcgtgatt
      121 ataaatacga tgataaagga aatataatag cctacgatga tgggactgat ctagaatatg
      181 aaactgagaa acttgacgaa atcaaatcaa aaatttatgg tgttctaagt ccgtctaaag
      241 atggacactt tgaaattctt ggaaagataa gtaatgtttc taaaaatgcc aaggtatatt
      301 atgggaataa ctataaatct atagaaatca aagcgaccaa gtatgatttc cactcaaaaa
      361 cgatgacatt tgatctatac gctaatatta atgatattgt ggatggatta gcttttgcag
      421 gagatatgag attatttgtt aaagataatg atcagaaaaa agctgaaatt aaaattagaa
      481 tgcctgaaaa aattaaggaa actaaatcag aatatcccta tgtatcaagt tatgggaatg
      541 tcatagaatt aggggaagga gatctttcaa aaaacaaacc agacaattta actaaaatgg
      601 aatctggtaa aatctattct gattcagaaa aacaacaata tctgttaaag gataacatca
      661 ttctaagaaa aggctatgca ctaaaagtga ctacctataa tcctggaaaa acggatatgt
      721 tagaaggaaa tggagtctat agcaaggaag atatagcaaa aatacaaaag gccaatccta
      781 atctaagagt cctttcagaa acaacaattt atgctgatag tagaaatgtt gaagatggaa
      841 gaagtaccca agctgtatta atgtcggctt tggacggctt taacattata aggtatcaag
      901 tgtttacatt taaaatgaac gataaagggg aagctatcga taaagacgga aatcttgtga
      961 cagattcttc taaacttgta ttatttggta aggatgataa agaatacact ggagaggata
     1021 agtccaatgt agaagctata aaagaagatg gctccatgtt atttattgat accaaaccag
     1081 taaacctttc aatggataag aactacttta atccatctaa atctaataaa atttatgtac
     1141 gaaatccaga attttattta agaggtaaga tttctgataa gggtggtttt aactgggaat
     1201 tgagagttaa tgaatcggtt gtagataatt atttaatcta cggagattta cacattgata
     1261 acactagaga ttttaatatt aagctgaatg ttaaagacgg tgacatcatg gactggggaa
     1321 tgaaagacta taaagcaaac ggatttccag ataaggtaac agatatggat ggaaatgttt
     1381 atcttcaaac tggctatagc aatttgaatg ctaaagcagt tggagtccac tatcagtttt
     1441 tatatgataa tgttaaaccc gaagtaaaca ttgatcctaa gggaaatact agtatcgaat
     1501 atgctgatgg aaaatctgta gtctttaaca tcaatgataa aagaaataat ggattcgatg
     1561 gtgagattca agaacaacat atttatataa atggaaaaga atatacatca tttgatgata
     1621 ttaaacaaat aacagacaag acactaaaca ttaagattgt tgtaaaagat tttgcaagaa
     1681 atacaaccgt aaaagaattc attttaaata aagatacggg agaggtaagt gaattaaaac
     1741 ctcatagggt aactgtgacc attcaaaatg gaaaagaaat gagttcaacg atagtgtcgg
     1801 aagaagattt tattttacct gtttataagg gtgaattaga aaaaggatac caatttgatg
     1861 gttgggaaat ttctggtttc gaaggtaaaa aagacgctgg ctatgttatt aatctatcaa
     1921 aagatacctt tataaaacct gtattcaaga aaatagagga gaaaaaggag gaagaaaata
     1981 aacctacttt tgatgtatcg aaaaagaaag ataacccaca agtaaaccat agtcaattaa
     2041 atgaaagtca cagaaaagag gatttacaaa gagaagatca ttcacaaaaa tctgattcaa
     2101 ctaaggatgt tacagctaca gttcttgata aaaacaatat cagtagtaaa tcaactacta
     2161 acaatcctaa taagttgcca aaaactggaa cagcaagcgg agcccagaca ctattagctg
     2221 ccggaataat gtttatagta ggaatttttc ttggattgaa gaaaaaaaat caagattaag
     2281 ataaaagcta tagaaaaaaa tggtttatgt actgagatta gatagtgagg tgatgacata
     2341 gttttgtgaa aacagccatt tataactcaa ttatttagtt tacttcacta ttgatactat
     2401 ttggagttat taatggactt agtttagata actaatgaat tgattgaaag ggttagtatt
     2461 gacaatattg gtcatattga ttagaaaata gagtctatca aaatttaaag gctaatagag
     2521 gtgatgagac aatttcaagg aattaagtag cgtttggttg cttaattcct ttttatttgg
     2581 aaggaagttt ttcgtaaccg cccaataacg aagtctattg gacggttacg atatgatcac
     2641 tgacttcgtc agttctatct acaacctaaa aacagtgttt tgaacaacct gcggctagct
     2701 ttctagtttg ctctttgatt ttcattgaat attagaacag caaaaatgct tggagtattt
     2761 gtttgtgtgt ttgtttttat ataacaaact ataaacaaaa caaaaatata aaaaaagaga
     2821 caaaaaagaa cagaaagtaa ttgacaacgg tttcatatag tgttatactt atggcataaa
     2881 gttaatggaa agaagggaaa accttatggt attagattat ttctttgaca aaaaccttgt
     2941 gttttgctta gaagcggata atcaagaaca actctttgat caggtagcaa gcttgttgga
     3001 agaacgagaa attgtcactc caacttatcg tgaagccttg atcacgcgtg aaaagtcatt
     3061 tccaactggt ttagatatgg aatttctagg aaaggacttg ccaaatgtag cgattcctca
     3121 cacagatatt gttcataatt tggctgaaaa agttgtggtc gttcgattag agaaaccggt
     3181 gactttccat aatatgattg cgccagataa ggaagtagaa gtatccttgc tcttctttat
     3241 catcaataac tcaagttcaa gtcaaacgaa cattcttgct cagttaatgg acttcttcac
     3301 agaaaatggt catcttgaag acctatcaaa aatttctgaa ccagaagccc tttatgctta
     3361 cattgctgaa gcaaccgctt aatcttgtct attaaaaaat aaaatcggag gaaatccaaa
     3421 tgattaaaat tcttgctgcc tgcggtgcag gtgttaactc aagtcaccaa attaaaagtg
     3481 ctctagaaga agaactttca aaccgtggtt atgatgttca ctgtgatgca gtcatggtga
     3541 aagatgtaaa cgaagacctt atgaaaggtt acgatatctt tacaccaatc gctgcaacag
     3601 accttggttt tgaaccaggt atcccagtta tcgaagctgg gccaatctta ttccgtatcc
     3661 cagcaatgag cgctccagta tttgacaata ttgaagcagc tattaaagaa cacggattaa
     3721 gctaattcta atttgttaac attcatataa cataaaaaag ggaggaactg ttatggatgc
     3781 aatctttgac ctaatcggaa aggttttcaa tcccatttta gaaatgggtg gacctgtcat
     3841 catgttaatc attttgacag tattggcttt actttttgga gtgaaattct ccaaagcgct
     3901 tgaaggtggt atcaaacttg ccatcgctct tacaggtatc ggtgctatca tcggtatgct
     3961 aaatggtgct ttctcagcat cacttgcaaa attcgttgaa aacactggta tccaattgag
     4021 tattaccgac gttggttggg caccactcgc aacaatcact tggggttctg cttggacact
     4081 atacttcttg ctcatcatgt tgattgtcaa cgtagtgatg cttgctatga agaaaaccga
     4141 tacacttgat gtcgatatct ttgatatctg gcacttgtct atcacaggtc tcttgattaa
     4201 atggtatgct gataacaatg gtgtgagtca aggggtttca ctctttattg ctacagcagc
     4261 tatcgtcctt gtcggtgtgt tgaaaattat caactctgac ttgatgaaac ctacatttga
     4321 tgaccttctt aacgccccaa gttcatcacc aatgacatca actcacatga actacatgat
     4381 gaacccagtt atcatggttt tggataagat ttttgaaaaa ttcttcccag gccttgataa
     4441 atatgacttt gatgctgcta aattgaacaa gaaaatcggt ttctggggat ctaaattttt
     4501 catcggtttc atccttggta tcgttatcgg tattatggga actccacatc caattgcagg
     4561 tgttgcagat gcagataaat ggcgtcttgt tatcaaagga tggttgtctc ttggtttgac
     4621 tgccggtgta tctttggaac tcttctcact tatcggttca tggttcatcg cagccgtaga
     4681 accactatca caaggtatta caaacgttgc tactaaacgt cttcaaggac gtaaattcaa
     4741 tatcggtctt gactggccat tcatcgctgg tcgtgctgaa atctgggctt gtgccaacgt
     4801 acttgcacca atcatgttga ttgaagcagt gcttctttca aaagttggaa atggtatctt
     4861 gccacttgca ggtatcatcg ctatgggtgt tactccagct ctcttggttg taactcgtgg
     4921 taaattgctc cgtatgatta tcttcggaac actcttgttg ccactcttcc ttctttcagg
     4981 tacacttatt gcaccatttg caacagaact tgctaaaggt gtaggtgcct tcccagaagg
     5041 tgtgagccaa actcaattga ttactcactc tactcttgaa ggaccaatcg aaaaacttct
     5101 tggttggaca attggtaaca ctacaactgg tgatatcaaa gcaatccttg gtgcagtagt
     5161 cttccttgta ttctatatcg gtatctttgc ttggtacaga aaacaaatga tcaaacgtaa
     5221 cgaagagtac gcagcaaaag caaaataatg cgctcctata aaatataaac tcaatgaaaa
     5281 tcaaagagca aactaggaag ctagccgcag gttgctcaaa acacagtttt gaggttgtag
     5341 ataagactga cgaagtcagc tcaaaacatg gttttgaggt tgtagatgaa actgacgaag
     5401 tcagctcaaa acatggtttt gaggttgtag atgaaactga cgaagtcagt aatcatatct
     5461 acggcaaggc gacactgacg aagtttgaat tggattttcg cagagtgtaa attgttgaaa
     5521 ctaggttgtc ataccatgta taccacttgg gctttggtag tagctaactg cactaaatat
     5581 aatataagga gggttttatg gggaaaggcc attggaatcg gaaaagagtt tatagcattc
     5641 gtaagtttgc tgtgggagct tgctcagtaa tgattgggac ttgtgcagtt ctattaggag
     5701 gaaatatagc tggagaatct gtagtttatg cggatgaaac acttattact catactgctg
     5761 agaaacctaa agaggaaaaa atgatagtag aagaaaaggc tgataaagct ttggaaacta
     5821 aaaatgtagt tgaaaggaca gaacaaagtg aacctagttc aactgaggct attgcatctg
     5881 agaagaaaga agatgaagcc gtaactccaa aagaggaaaa agtgtctgct aaaccggaag
     5941 aaaaagctcc aaggatagaa tcacaagctt caagtcaaga aaaaccgctc aaggaagatg
     6001 ctaaagctgt aacaaatgaa gaagtgaatc aaatgattga agacaggaaa gtggatttta
     6061 atcaaaattg gtactttaaa ctcaatgcaa attctaagga agccattaaa cctgatgcag
     6121 acgtatctac gtggaaaaaa ttagatttac cgtatgactg gagtatcttt aacgatttcg
     6181 atcatgaatc tcctgcacaa aatgaaggtg gacagctcaa cggtggggaa gcttggtatc
     6241 gcaagacttt caaactagat gaaaaagacc tcaagaaaaa tgttcgcctt acttttgatg
     6301 gcgtctacat ggattctcaa gtttatgtca atggtcagtt agtggggcat tatccaaatg
     6361 gttataacca gttctcatac gatatcacca aataccttca caaagatggt cgtgagaatg
     6421 tgattgctgt ccatgcagtc aacaaacagc caagtagccg ttggtattca ggaagtggta
     6481 tctatcgtga tgtgacttta caagtgacag ataaggtgca tgttgagaaa aatgggacaa
     6541 ctattttaac accaaaactt gaagaacaac aacatggcaa ggttgaaact catgtgacca
     6601 gcaaaatcgt caatacggac gacaaagacc atgaacttgt agccgaatat caaatcgttg
     6661 aacgaggtgg tcatgctgta acaggcttag ttcgtacagc gagtcgtacc ttaaaagcac
     6721 atgaatcaac aagcctagat gcgattttag aagttgaaag accaaaactc tggaccgttt
     6781 taaatgacaa acctgccttg tacgaattga ttacgcgtgt ttaccgtgac ggtcaattgg
     6841 ttgatgctaa gaaggatttg tttggttacc gttactatca ctggactcca aatgaaggtt
     6901 tctctttgaa tggtgaacgt attaaattcc atggagtatc cttgcaccac gaccatgggg
     6961 cgcttggagc agaagaaaac tataaagcag aatatcgccg tctcaaacaa atgaaggaga
     7021 tgggagttaa ctccatccgt acaacccaca accctgctag tgagcaaacc ttgcaaatcg
     7081 cagcagaact aggtttactc gttcaggaag aggcttttga tacttggtat ggtggcaaga
     7141 aaccttatga ctatggacgt ttctttgaaa aagatgccac tcacccagaa gctcgaaaag
     7201 gtgaaaaatg gtctgatttt gacctacgta ccatggtcga aagaggcaaa aacaaccctg
     7261 ctatcttcat gtggtcaatt ggtaatgaaa taggtgaagc taatggtgat gcccactctt
     7321 tagcaactgt taaacgtttg gtcaaggtta tcaaggatgt tgataagact cgctatgtta
     7381 ccatgggagc agataaattc cgtttcggta atggtagcgg agggcatgag aaaattgctg
     7441 atgaactcga tgctgttgga tttaactatt ctgaagataa ttacaaagcc cttagagcta
     7501 agcatccaaa atggttgatt tacggttcag aaacatcatc agcaacccgt acacgtggaa
     7561 gttactatcg ccctgaacgt gaattgaaac atagcaatgg acctgagcgt aattatgaac
     7621 agtcagatta tggaaatgat cgtgtgggtt gggggaaaac agcaaccgct tcatggactt
     7681 ttgaccgtga caacgctggc tatgctggac agtttatctg gacaggtacg gactatattg
     7741 gtgaacctac accatggcac aaccaaaatc aaactcctgt taagagctct tactttggta
     7801 tcgtagatac agccggcatt ccaaaacatg acttctatct ctaccaaagc caatgggttt
     7861 ctgttaagaa gaaaccgatg gtacaccttc ttcctcactg gaactgggaa aacaaagaat
     7921 tagcatccaa agtagctgac tcagaaggta agattccagt tcgtgcttat tcgaatgctt
     7981 ctagtgtaga attgttcttg aatggaaaat ctcttggtct taagactttc aataaaaaac
     8041 aaaccagcga tgggcggact taccaagaag gtgcaaatgc taatgaactt tatcttgaat
     8101 ggaaagttgc ctatcaacca ggtaccttgg aagcaattgc tcgtgatgaa tctggcaagg
     8161 aaattgctcg agataagatt acgactgctg gtaagccagc ggcagttcgt cttattaagg
     8221 aagaccatgc gattgcagca gatggaaaag acttgactta catctactat gaaattgttg
     8281 acagccaggg gaatgtggtt ccaactgcta ataatctggt tcgcttccaa ttgcatggcc
     8341 aaggtcaact ggtcggtgta gataacggag aacaagccag ccgtgaacgc tataaggcgc
     8401 aagcagatgg ttcttggatt cgtaaagcat ttaatggtaa aggtgttgcc attgtcaaat
     8461 caactgaaca agcagggaaa ttcaccctta ctgcccactc tgatctcttg aaatcgaacc
     8521 aagtcactgt ctttactggt aagaaagaag gacaagaaaa gactgttttg gggacagagg
     8581 tgccaaaagt acagaccatt attggagagg cacctgaaat gcctaccact gttccgtttg
     8641 tatacagtga tggtagtcgt gcagaacgtc ctgtaacctg gtctttagta gatgtgagca
     8701 agcctggtat tgtaacggtg aaaggtatgg ctgacggacg agaagtagaa gctcgtgtag
     8761 aagtgattgc tcttaaatca gagctaccag ttgtgaaacg tattgctcca aatactaact
     8821 tgaattctgt agacaaatct gtttcctatg ttttgactga tggaagtgta caagagtatg
     8881 aagtggacaa gtgggagatt gccgaagaag ataaagctaa gttagcaatt ccaggttctc
     8941 gtattcaagc gaccggttat ttagaaggtc aaccaattca tgcaaccctt gtggtagaag
     9001 aaggcaatcc tgcagcacct gtagtgccaa ctgttactgt tggaggtgaa gcagtaacag
     9061 gtcttactag tcgacaacca atgcaatatc gtactctatc ttatggtgcc caattgccag
     9121 aagtcacagc aagtgctgaa aatgctgatg tgacagttct tcaagcaagc gcagcaaacg
     9181 gcatgcgtgc gagcatcttt attcagccta aagatggtgg ccctcttcaa acctatgcaa
     9241 ttcaattcct tgaagaagcg ccaaaaattg ctcacttgag cttgcaagtg gaaaaagctg
     9301 acagtctcaa agaagaccaa actgtcaaat tgtcggttcg agctcactat caagatggaa
     9361 cgcaagctgt attaccagct gataaagtaa ccttctctac aagtggtgaa ggggaagtcg
     9421 caattcgtaa aggaatgctt gagttgcata agccaggagc agtcactctg aacgctgaat
     9481 atgagggagc taaaggccaa gttgaactca ctatccaagc caatactgag aagaagattg
     9541 cgcaatctat ccgtcctgta aatgtagtca cagatttgca tcaggaacca agtcttccag
     9601 caacagtaac agttgagtat gacaaaggtt tccctaaaac tcataaagtc acttggcaag
     9661 ctattccgaa agaaaaacta gactcctatc aaatatttga agtactaggt aaagttgaag
     9721 gaattgacct tgaagcgcgt gcaaaagtct ctgtagaagg tatcgtttca gttgaagaag
     9781 tcagtgtgac aactccaatc gcagaagcac cacaattacc agaaagcgtt cggacatatg
     9841 attcaaatgg tcacgtttca tcagctaagg ttgcatggga tgcgattcgt ccagagcaat
     9901 acgctaagga aggtgtcttt acagttaatg gtcgcttaga aggtactcaa ttaacaacta
     9961 aacttcatgt tcgcgtatct gctcaaactg agcaaggtgc aaacatttct gaccaatgga
    10021 ccggttcaga attgccactt gcctttgctt cagattcaaa tccaagcgac ccagtttcaa
    10081 atgttaatga caagctcatt tcctacaata accaaccagc caatcgttgg acaaactgga
    10141 atcgtagtaa tccagaagct tcagtcggtg ttctgtttgg agattcaggt atcttgagca
    10201 aacgctccgt tgataatcta agtgtcggat tccacgaaga ccatggagtt ggtgcaccga
    10261 agtcttatgt gattgagtat tatgttggca agactgtccc aacagctcct aaaaacccta
    10321 gttttgttgg taatgaggac catgtcttta atgattctgc caactggaaa ccagttacta
    10381 atctaaaagc ccctgctaaa ctcaaggctg gagaaatgaa ccactttagc tttgataaag
    10441 ttgaaaccta tgctgttcgt attcgcatgg ttaaagcaga taacaagcgt ggaacgtcta
    10501 tcacagaggt acaaatcttt gcgaaacaag ttgcggcagc caagcaagga caaacaagaa
    10561 tccaagttga cggcaaagac ttagcaaact tcaaccctga tttgacagac tactaccttg
    10621 agtctgtaga tggaaaagtt ccggcagtca cagcaagtgt tagcaacaat ggtctcgcta
    10681 ccgtcgttcc aagcgttcgt gaaggtgagc cagttcgtgt catcgcgaaa gctgaaaatg
    10741 gcgatatctt aggagaatac cgtctgcact tcactaagga taagaactta ctttctcata
    10801 aaccagttgc tgcggttaaa caagctcgct tgctacaagt aggtcaagca cttgaattgc
    10861 cgactaaggt tccagtttac ttcacaggta aagacagcta cgaaacaaaa gacctgacag
    10921 ttgaatggga agaagttcca gcggaaaatc tgacaaaagc aggtcaattt actattcgag
    10981 gccgtgtcct tggtagtgac cttgttgctg aggtcactgt acgagtgaca gacaaacttg
    11041 gtgaggctct ttcagataac cctaactatg atgaaaacag taaccaggcc tttgcttcag
    11101 caacaaatga tattgacaaa aactctcatg accgcgttga ctatctcaat gacggagatc
    11161 attcagaaaa tcgtcgttgg acaaactggt caccaacacc atcttctaat ccagaagtat
    11221 cagcgggtgt gatcttccgt gaaaatggta agattgtaga acggactgtt gctcaagcca
    11281 aacttcactt ctttgcagat agtggtacgg atgcaccaac taaactcgtt ttagaacgct
    11341 atgtcggtcc agagtttgaa gtgccaacct actattcaaa ctaccaagcc tacgacgcag
    11401 accatccatt caacaatcca gaaaattggg aagctgtgcc ttatcgtgcg gataaagaca
    11461 tcgcagctgg tgatgaaatc aacgtaacat ttaaagctgt caaagccaaa gctatgagat
    11521 ggcgtatgga gcgtaaagca gataagagcg gtgttgcgat gattgagatg accttccttg
    11581 caccaagtga attgcctcaa gaaagcacgc aatcgaagat tcttgtagat ggaaaagaac
    11641 ttgctgattt cgctgaaaat cgtcaagact atcaaattac ctataaaggt caacggccaa
    11701 aagtctcagt tgaagaaaac aatcaagtag cttcaactgt ggtagatagt ggagaagata
    11761 gccttccagt acttgttcgc ctcgtttcag aaagtggaaa acaagtcaag gaataccgta
    11821 tccacttgac taaggaaaaa ccagtttctg agaagacagt tgctgctgta caagaagatc
    11881 ttccaaaact cgaatttgtt gaaaaagatt tggcctacaa gacagttgag aaaaaagatt
    11941 caacactgta tctaggtgaa actcgtgtag aacaagaagg aaaaactggt aaagaacgta
    12001 tctttacagc gattaatcct gatggaagta aggaagaaaa actccgtgaa gtggtagaag
    12061 ctccgacaga ccgcatcgtc ttggttggaa ccaaaccagt agctcaagaa gctaaaaaac
    12121 cacaagtgtc agaaaaagca gatacaaaac caattgattc aagtgaagct agtcaaacta
    12181 ataaagccca gttaccaaat acaggtagtg cggcaagcca agcagcagta gcagcaggtt
    12241 tagctcttct aggtttgagt gcaggattag tagttactaa aggtaaaaaa gaagactaga
    12301 aattttgata ccttctttat cattaaagac caaagaaaaa ctcttgtcct aaatagaaga
    12361 taaagagtct ggaacaaaat agttctcaac cacaaaaaag ctagatattt ccaattgcgg
    12421 aactctagct ttttaatttt gagtcgttct cttattgtat agtagattga aactagagta
    12481 gtacacttct acttctaaaa cattgttaga aatcgatttg actgtcctta tcgatttgtc
    12541 ctgttcttat ttcatttcac tatgttttag gaggttattg gccataagta ccaatcccat
    12601 gtcaattctc acttgacgct tccctctcag attacatctc ttgtaaccca aacaagcctt
    12661 tatctgccca aagacaggtt ccacatcaat cttgcgttga gcgaaaatct gtctatcttg
    12721 gggagataaa agcgcctgac attctttagc tttcaagttt tgatagcgtt cgttcatata
    12781 cagtcccttt tgaggggctg attcaggttc atcagcgtag taaaccttga tttcctgttg
    12841 aaagtctgtc tgtgttttct ggtacttgat atggtgaaaa cgatagcacc agccatcagg
    12901 atgtatgtag ctatcctcct tgtcattata gtgccaattc gctaagtttt tagctgactg
    12961 tttataccct ctcttttgtt tcttatcaaa catggcatat ttaatcagaa ggggagtgat
    13021 ttcttcttga aaatagactt gtatctgttc ttgaagcttg gcggagaatt tctctgtcgt
    13081 tttcttccac acaaaactat acttgttggc attggcttca atcttagttc cgtcaataaa
    13141 caaacaatct aaggtcacta actcttccat ttttcaattt ttcgaccaga gaaaatccct
    13201 tgtgaatagg caaatagaag agtagataca agcattttag ggtgataaga cgggcgacca
    13261 aaggcatgat agaaggcgta gaagtgacgt tcctccaagg tattcaccac tttttcaata
    13321 gtaaagacga gatggtcttg tggtaagaag gaactgattt ctagtggtaa agttgtttga
    13381 tttgtgttat agtgaatatg catgggatag cctttctaaa tggtttatta agcaattcta
    13441 ttttaccaag actatcgtaa ccatgcaaaa aagcaacggc aaatccgttg cctttttgga
    13501 tataagagac tattgtccca gactcttttc tttgtataaa attgtatagt gttattgtct
    13561 atactatttt gtagagaaag caaaaatccc tagaaaatgc ttagggattc gactatttca
    13621 ttaagaacaa ttcaacaatc atttctatat acatgataag ggttaataag aatcctgcac
    13681 gccagaagaa tttgatgaat ttaggatagt aaaagctgcg ttttttcaaa aggaagaaaa
    13741 agacgagaat aatggctagg aacgagaggg ctagccccag tctagggagg aaattatggg
    13801 taaaggtttt agctgtaatc aagtaatact caaataccaa gactggaaaa gccaaatccg
    13861 caaagtttcg tcctagtttt tttaatctaa aaagtttggt taggataatg cagacgacta
    13921 aggtcagtat caataataaa atagatgcta atttcattaa aatcataccc atattgtatc
    13981 ataaaaaaag gctaatggaa acgagaaaca gaggaattta taggaaagtc aggcttttga
    14041 gattgtgggt gatttttgtt ataatgaaag ttataaaatc ttataatacc ttgaatgatt
    14101 attatcgaaa actctttgga gaaaagactt ttaaagtccc tattgatgcg ggatttgact
    14161 gtcccaatcg tgatggaact gtggctcatg gaggctgtac tttttgtacg gtttccggtt
    14221 ctggagatgc cattgtggca ccagatgcgc ctatccgtga gcaattttat aaggaaattg
    14281 actttattca tcgtaagtgg ccagatgttc agaagtatct ggtttatttt caaaatttta
    14341 ccaacaccca tgaaaaggtg gaagttattc gagagcgtta tgaacaggct atcaatgagc
    14401 caggtgtggt aggaatcaat attggaacgc gcccagactg tttaccagac gaaaccatcg
    14461 aatatttggc tgagttatcg gagtgcatgc atgtgacggt tgaattgggc ttgcaaacta
    14521 cttatgaagc aacctctgac ctgattaacc gtgcgcattc ttatgaattg taggtggaaa
    14581 cggtcaagcg tttgagaaag tatccaaaaa ttgagattgt ttcccatttg atcaatggtt
    14641 tgcctggtga aacccatgag atgatggttg aaaatgtccg ccgttgtgtc acggataacg
    14701 atattcaagg gattaaactg catttgctcc atcttatgac aaatactcgt atgcaacgag
    14761 attaccatga ggggcgttta caattgatga gtcaggacga atatgtcagg gtcatctgtg
    14821 accaactgga aattattccc aaacatatcg tcatccatcg aattacagga gatgcgccta
    14881 gggatatgct gcttgggcct atgtggagcc tcaataaatg ggaagctctc aacagcattg
    14941 agatggagat gcgacgtcgt ggaagtgttc aaggatgcaa ggctgtaaaa caggagtttg
    15001 aaaatgaaaa gaccacttga gatggcacat gattttttgg ctgaggtcgt gacaaaagag
    15061 gatgtcgtag tggatgcgac tatgggaaat ggtcatgaca cgcttttttt agccaagcta
    15121 gccaagcaag tctatgcctt tgatattcag aagcaagcct tggaaaagac ccaagagcgt
    15181 ttgcatcagg ctgacttgac aaatgcccag ttaatcttgc aaggccatga gacactggac
    15241 cagtttgtga taaaagctaa ggcagggatt tttaatctgg gctatttgcc gtcagctgat
    15301 aagtctgtca tcacccgacc gcagacaacg attgaggcat tagaaaagct atgtggctta
    15361 cttgtcaaag gtggacgaat tgctattatg atttactatg gtcatgaagg aggcgatctc
    15421 gagagagatg ctgtcttgga ttttgtgatc cagttgaacc aacaagagta cacagctgcc
    15481 atttaccgaa ctttaaacca agtcaacaac ccgccgtttt tagtgatgat tgaaaaatta
    15541 gagagataca gacatggata aacaatacct acacgaaaag ctggatgcca tgcgccagaa
    15601 ttttgttgaa tcaacccacc acgaacgatc ggtgggcgtg ctagaccaag cgcatatgag
    15661 caaaaaaatg cttaaaatca agaaaaaatt agttgctctt gaaatggaac ggtgccagag
    15721 aaaaattgag cacaaagact gttccaagat tgaccaaaaa atcaaagagc agaaggagat
    15781 atttgaatcc tgttgtaaaa aagattaagg aggacgtgcg tggaattact gatttacctc
    15841 atcctatttt tactggtttt gattgtctca agtacaacca ataagctcct gccctttttg
    15901 cctctccctt tggtgcagat tcttttggga attgtgattg gtctcttttt acccaatact
    15961 gactttcacc ttaatacgga gttgtttttg gcactggtta tcggaccctt gcttttccga
    16021 gaggctgaag aagcagatgt tacggctatt ttaaaacact ggcgaatcat tgtttatctc
    16081 atatttccag tgatttttat ctcgaccttg agtttgggtg gcttggccca tcttctttgg
    16141 ttcagccttc ccttggcagc ttgcttggct gttggggcag cccttggtcc tacggacttg
    16201 gtggcctttg cctctctttc ggagcgtttt agctttccta agcgcgtgtc caatattctt
    16261 aagggcgaag gactcttgaa tgatgcttct ggtttggtgg cttttcaggt agctttgaca
    16321 gcttggacaa ctggagcttt ttctctgggg caagctagca gttcgctcat cttttcaatc
    16381 ctaggcggtt ttttaattgg atttttaacg gccatgacca accgcttcct ccataccttc
    16441 ttgctaagtg tgcgcgcaac ggatattgcc agtgaacttt tattagaatt gagtttgcct
    16501 ctagtgacct tctttctggc agaagaagtt catgtttcag gtattattgc cgtcgtagtt
    16561 gctggaattt taaaggcaag tcgcttcaag aaaatcacgc tcctcgaagc ccaagtggat
    16621 acggtgaccg agacggtctg gcatacagtg acctttatgc tcaacggttc tgtctttgtg
    16681 attttaggga tggagttgga aatgatagca gaacctatct tgaccaatcc aatctataat
    16741 cctctacttt tattgctatc tctcatcgcc cttacctttg tcctctttgt cattcgtttt
    16801 attatgatct atggctatta tgcctataga acccgacgcc tcaagaaaaa gctaaataag
    16861 tatatgaagg acatgtttct cttgaccttt tcaggtgtta agggaacggt gtcgattgct
    16921 acgattctct tgataccaag taatctagaa caggagtatc ctcttttgct tttccttgtt
    16981 gcaggtgtga cgcttgtcag ctttttaaca ggtctcttgg tcttgcctca tctttctgat
    17041 gaagaggaag aaagcaagga ttatctcatg catatcgcca ttttgaatga agtaacgcta
    17101 gagttggaaa aagagttgga agacaccaga aataaacttc ccctctatgc ggctattgac
    17161 aattatcatg gacgtattga aaatctcatt ttaagccaag aaaaccagga tgatcaagaa
    17221 gattgggctg ctttgaaact cttgattctt agtattgaaa gtgatggttt ggaacaggcc
    17281 tatgaagagg ggaacattag caatcgtgct taccgagttt accaacgtta tctgaaaaat
    17341 atagaacaag gaatcaatcg taaacttgcc tcaagactga cctattattt tcttgtttcc
    17401 ttgaggattt tacgttttct ccttcatgaa gtttttactc ttggaaagac cttccgtagc
    17461 tggaaggaca aggagcaaag ccgtctccgt gctcttgatt atgaccaaat tgcagagctc
    17521 tatcttgcca atacagagat gattattgaa agtttggaaa acctgaaggg agtctacaga
    17581 cgctctttga ttagttttat gcaggagtct cgtcttcgag aaacagctat tatcagcagt
    17641 ggtgcctttg tcgaacgggt tatcaatcgt gtcaaaccca acaatatcga tgaaatgctg
    17701 agaggctatt atctggagcg caagttgatt ttcgaatacg aagaaaaacg attgattacg
    17761 actaagtatg ccaagaaatt acgacaaaat gtaaataact tagagaacta ttccttgaag
    17821 gaagctgcca ataccctgcc gtatgatatg gtggaattgg taagaagaaa ttagttaata
    17881 ctcttcgaaa atctcttcaa accacgtcag cgtcgccttg gattatatat gtgactgact
    17941 tcgtcagttt catctacaac ctcaaagcag ggctttgagc aacctgcggc tagcttccta
    18001 gtttgctctt tgattttcat tgagtataag attataaatg aaggagtatg acatgaaaaa
    18061 gtggtggaaa gagctgatag acaagccttt attaaaagct tttttgcatt attatcaagc
    18121 atcagatagt gagttgacca gtgtcgcagt agcctactat tggttgattt cgattttccc
    18181 cctgcttttg gtggtggtca atatcctccc ctattttcag attcctgtgg gagaatttct
    18241 gtgctttatg aaagacgttc tgcccccaag cctctatgaa ggtgtggaaa aaatagccag
    18301 agaagttttg acgcaacctt caacaggttt attgagtttt tctgttttat cggcgttatg
    18361 gagtttttca aaatctatga atttcttgca aaaagctttt aacaaggctt atggcgtcga
    18421 aaagagtcgt ggacttattt cccatcagat gctaagtctc ctagtcagtt ttggcttgca
    18481 gctcctcttt gcgtttgcct tgtttttgat cttatttggt cagatgattt tggctttact
    18541 tgctcattat tggacaaaag atggcattat ctatcaggct ttacaagggt tggcaggtcc
    18601 tctgatttac gccctcctct ttgctatctt ggtcatgctt tattattttc ttcccaatct
    18661 atctaacagg aaaattagct ataccctacc aggcagtgcc tttgttctct tagtgatttt
    18721 aggtttacta actctgttct ctagttatct caattactat gtccatcatt tggtggatgt
    18781 ccgaatttta ggttccgttc ttcttgttgt tatgatgttt tggtttatct tgattgctaa
    18841 aattgttatc ctaggcgcgg ttatcaatgc cagtatgcag agtttgaaag atccggtctt
    18901 taaaaaagac taactattta tccattacaa aagcgcatag tatcaaaact ttatagaacc
    18961 ttgatactat gcgcttttgt gctttgacag ttttttgtat agtttttgtc caagatttct
    19021 aaatcatctg attaaattcc tattttcccc tatcattgtc cctgtttttt cgaaaatgga
    19081 aggagtataa taaatctatc aataaaatga taggaggaac tttatgggtc atattttctt
    19141 ttttctaagt gtctttttgg cagggattct atccttcttt tctccttgta tcttaccttt
    19201 gttaccggtc tatacaggag tgttactaga tgataaggat ggtgctcagg cttctagcgg
    19261 caaattttca atctcagtta ctagtttatt acgaactctg gcctttatag caggaatttc
    19321 ctttatattt attttgttgg gctatggagc tggtttttta ggcgatttgc tttatgcttc
    19381 ttggttccaa tatcttactg gggcaattat tatccttctt ggtttgcacc aaatggagat
    19441 tctacacttt aaggggcttt ataaggaaaa gaggctacaa ctgcaaggac aggggcaaaa
    19501 tggtaagggc tatagtcagg catttttatt gggcttgacc tttagttttg cttggacgcc
    19561 ttgcgtgggg ccggttctgg ggtctgtttt ggccttggcg gcttcaggtg gttcaggagc
    19621 ttggcaggga gctggtctca tgttggtgta tacgctgggc ttggcgctac cattcttgct
    19681 tctagctctg acctctagtt atgttttgaa acatttccga aaacttcatc cctatctcgg
    19741 aatcctcaaa aaagtgggtg gttttctcat tattgtgatg ggactcttgg ttctgtttgg
    19801 aaatgcttca attttaagtc aattatttga ataaaatgga aaggaatatc aatatgaaaa
    19861 aatggcaaac atgtgttctt ggagcaggtt cgctcctttg tttgacggct tgttcaggca
    19921 agtccgtgac tagtgaacac caaacgaaag atgaaatgaa gacggagcag acagctagta
    19981 aaacaagcgc agctaaaggg aaagaggtgg ctgattttga attgatggga gtagatggca
    20041 agacctaccg tttatctgat tacaagggca agaaagtcta tctcaaattc tgggcttctt
    20101 ggtgttccat ctgtctggct agtcttccag atacggatga gattgctaaa gaagctggtg
    20161 atgactatgt ggtcttgaca gtagtgtcac caggacataa gggagagcaa tctgaagcgg
    20221 actttaagaa ttggtataag ggattggatt ataaaaatct cccagtccta gttgacccat
    20281 caggcaaact tttggaaact tatggtgtcc gttcttaccc aacccaagcc tttatagaca
    20341 aagaaggcaa gttggtcaaa acacatccag gattcatgga aaaagatgca attttgcaaa
    20401 ctttgaagga attagcctag gaggtgtctt atgaatgata agttaaaaat cttcttgttg
    20461 ctaggagtat tttttctagc cataaccggt ttctatgttc tattgatacg aaatgcaggg
    20521 cagacagatg cctcgcaaat tgaaaaggcg gcagttagcc aaggaggaaa agcagtgaaa
    20581 aaaacagaaa ttagtaaaga cgcagacttg cacgaaattt atctagctgg aggttgtttc
    20641 tggggagtgg aggaatattt ctcacgcgtt cccggggtga cggatgccgt ttcaggctat
    20701 gcaaatggta gaggagaaac aaccaagtac gaattgatta accaaacagg tcatgcagaa
    20761 accgtccatg tcacctatga tgccaagcaa atttctctca aggaaatcct gcttcactat
    20821 ttccgcatta tcaatccaac cagcaaaaat aaacaaggaa atgatgtggg gacccagtac
    20881 cgtactggtg tttattacac agatgacaag gatttggaag tgattaacca agtctttgat
    20941 gaggtggcta agaaatacga tcaacctcta gcagttgaaa aggaaaactt gaagaatttt
    21001 gtggtggctg aggattacca tcaagactat ctcaagaaaa atccaaatgg ctactgccat
    21061 atcaatgtta atcaggcggc ctatcctgtc attgatgcca gcaaatatcc aaaaccaagt
    21121 gatgaggaat tgaaaaagac cctgtcacct gaggagtatg cagttaccca ggaaaatcaa
    21181 acagaacgag ctttctcaaa ccgttactgg gataaatttg aatccggtat ctatgtggat
    21241 atagcaactg gggaacctct cttttcatca aaagacaaat ttgagtctgg ttgtggctgg
    21301 cctagtttta cccaacccat cagtccagat gttgtcacct acaaggaaga taagtcctac
    21361 aatatgacgc gtatggaagt gcggagccga gtaggagatt ctcaccttgg gcatgtcttt
    21421 acggatggtc cacaggacaa gggcggctta cgttactgta tcaatagcct ctctatccgc
    21481 tttattccca aagaccaaat ggaagaaaaa ggctacgctt atttactaga ttatgttgat
    21541 taatactctt cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgta tgtagggtta
    21601 ctgactccgt cagttttatc tacaacctca aagcggtact ttgagaaacc tgtggctatc
    21661 ttcctagttt actttttgat ttttattgag tataagaggt ctttcctaag cagttagagg
    21721 agaattttgc tatactgata ctagtaagtg gtaaaggagc agatcatgac ctacacaatc
    21781 ttaatcgtag aagatgaata tctggtaaga caaggtttga ctaaactggt caatgtagca
    21841 gcctacgata tggaaatcat cggtcaggct gaaaatggaa ggcaggcttg ggaattgatc
    21901 caaaagcagg tgccagatat cattttaacc gatatcaaca tgcctcatct aaatggcatc
    21961 cagttggcca gtctggtacg agaaacctat cctcaggttc atttggtctt tttaacaggt
    22021 tacgatgatt ttgattatgc cttgtctgct gtcaaactag gtgtggacga ctacctactc
    22081 aaaccctttt ctcgtcagga tattgaggaa atgttgggga aaatcaaaca aaaactagac
    22141 aaggaagaga aagaagagca gttacaagat ttattgacta ataggtttga aggaaacatg
    22201 gcccagaaaa tccagtctca tctggctgat agccaattta gtttaaagtc tttagccagt
    22261 gacttaggtt ttagtccgac ctatctgagt tccttgatta agaaagagtt gggcttgcct
    22321 tttcaggatt atctggtgag agaacgtgtt aaacaagcca agctcttgct tttaactaca
    22381 gatttgaaga tttatgagat cgcagagaag gttggttttg aagatatgaa ctattttacc
    22441 caacgtttta agcagattgc aggtgtaaca cctcgtcagt ttaagaaggg agaagaccga
    22501 tgaagcgttc ttctctttta gttagaatgg ttatttccat ctttctggtc tttctcattc
    22561 tcctagctct ggttggaact ttctactatc aatcaagttc ttcagccatt gaggccacca
    22621 ttgagggcaa cagccaaacg accatcagcc agactagcca ctttattcag tcttatatca
    22681 aaaaactaga aaccacctcg actggtttga cccagcagac ggatgttctg gcctatgctg
    22741 agaatcccag tcaagacaag gtcgagggaa tccgagattt gtttttgacc atcttgaagt
    22801 cagataagga cttgaaaact gttgtgctgg tgaccaaatc tggtcaggtc atttctacag
    22861 atgacagtgt gcagatgaaa acttcctctg atatgatggc tgaggattgg taccaaaagg
    22921 ccattcatca gggagctatg cctgttttga ctccagctcg taaatcagat agtcagtggg
    22981 tcatttctgt cactcaagaa cttgttgatg caaagggagc caatcttggt gtgcttcgtt
    23041 tggatatttc ttatgaaact ctggaagcct atctcaatca actccagttg gggcagcagg
    23101 gctttgcctt cattatcaat gaaaaccatg aatttgtcta ccatcctcaa cacacagttt
    23161 atagttcgtc tagcaaaatg gaggctatga aaccctacat cgagacaggt cagggttata
    23221 ctcctggtca caaatcctac gtcagtcaag agaagattgc aggaactgat tggacggtac
    23281 ttggcgtgtc atcattggaa aagttagacc aggttcggag tcagctcttg tggaccttgc
    23341 ttggggccag tgtcacatct cttcttgttt gtctctgctt agtgtggttc agtcttaaac
    23401 gctggattgc tcctttgaag gatttgagag aaaccatgtt ggaaattgct tctggtgctc
    23461 aaaatcttcg tgccaaggaa gttggtgcct atgaactgag agaagtaact cgccaattta
    23521 atgccatgtt ggatcagatt gatcagttga tggtagctat tcgtagccag gaagaaacga
    23581 cccgtcagta ccaacttcaa gccctttcga gccagattaa tccacatttc ctctataaca
    23641 ctttggacac catcatctgg atggctgaat ttcatgatag tcagcgagtg gtgcaggtga
    23701 ccaagtcctt ggcaacctat ttccgcttgg cgctcaatca aggcaaggac ttgatttgtc
    23761 tctctgacga aatcaatcat gtccgccagt atctctttat ccagaaacaa cgctatggag
    23821 ataagctgga atatgaaatt aatgaaaatg ttgcctttga taatttagtc ttacccaagc
    23881 tggtcctaca accccttgta gaaaatgctc tttaccatgg cattaaggaa aaggaaggtc
    23941 agggccatat taaactttct gtccagaaac aggattcggg attggtcatc cgtattgagg
    24001 atgatggcgt tggcttccaa gatgctggtg atagtagtca aagtcaactc aaacgtgggg
    24061 gagttggtct tcaaaatgtc gatcaacggc tcaaacttca ttttgaagcc aattaccaga
    24121 tgaagattga ttctagaccc caaaaaggga cgaaagttga aatatatata aatagaatag
    24181 aaactagcta actcccagtc aattctggga gttttatgcg taattgggca aactttctag
    24241 gttgtctatc ttgctaaaaa caagaaaaaa cggtatgata gaagagtgac aacagaggta
    24301 acagagatga acgagacaca gattcaaaga gaaacacgtc aggtagtgga agatgtttta
    24361 gaaaagacca atttgaagca gggggccctt tttgttttag gtctatcttc tagtgaggtg
    24421 ctaggtggtc agattgggaa agaatccagt caagagattg gtgagctgat agtggagact
    24481 atcttgggta ttctaggtag tagaggtatc catttagcag ttcaaggttg tgagcatgtc
    24541 aatcgggctc tcgttgttga acgtcaggtg gcagagcagt ttgatctgga aattgtcagt
    24601 gtccatccta ctcttcatgc aggaggttcg gggcagttgg cagcctttaa gtttatgcag
    24661 gatccagttg aggttgaatt tatcaaggct catgctggat tggatatcgg agacactgca
    24721 attggcatgc atgtcaagca tgttcaggtt ccgattcgcc ctattttgag agagattggt
    24781 catgcccatg taacggcact ggctagtcgt ccaaaattaa tcggaggtgc gcgtgcgcac
    24841 tatccgcaag atgctattag gaagtcttga gaatgaataa aggagataaa agtgtttaaa
    24901 aaagaccgtt tttcaattcg taagattaag ggagttgtag gctctgtatt tcttggaagc
    24961 cttttgatgg ctccttctgt agtggacgca gccacctatc actatgtaaa taaagagatt
    25021 atttcacaag aagctaaaga tttaattcag acaggaaagc ctgacaggaa tgaagttgta
    25081 tatggtttgg tgtatcaaaa agatcagttg cctcaaacag ggacagaagc atctgttttg
    25141 acagcttttg gtttgctgac tgttgggagc ttgcttttaa tctacaagag aaagaaaatt
    25201 gctagcgtct ttctagttgg agctatggga ttggtagttc ttcctagtgc aggggctgta
    25261 gacccagttg cgaccctagc gctggctagt cgagagggtg ttgttgaaat ggatggctat
    25321 cgctatgttg gttatctatc aggtgacatc ctcaaaacgc ttggcttgga cactgtttta
    25381 gaagaaacct cagcaaaacc tggagaggtg actgtagtcg aagttgagac tcctcaatca
    25441 acaacaaatc aggagcaagc taggacagaa aaccaagtag tagagacaga ggaagctcca
    25501 aaagaagaag cacctagaac agaagaaagt ccaaaggaag aaccaaaatc ggaggtaaaa
    25561 cctactgacg acacccttcc taaagtagaa gaggggaaag aagattcagt agaatcagct
    25621 ccagttgaag aagtaggtgg agcagttgat tcaaaaacag atgaacaagc accagtcaaa
    25681 tcggaaagtc aaccatcaga caaaccagct gaggaatcaa aagttgaacc atcagtagaa
    25741 ccagtcgcgc caagagaaga cgaaaaggca ccaacggcac cagttgagcc agaaaagcaa
    25801 ccagaagttc ctgaagaaac tgtagaacca aaagaggaga ctaaaacagc taaaggtacg
    25861 caagaaaaag gcaaagaagg tcaagcacca gtacaagaag tacatccaga atataaagta
    25921 acaacaggta cagttgagaa gtctattgaa agtgaattag atttcacaac agacgtagtt
    25981 ccggatgaca caaaatatgt gggtgacgaa gtagttgaaa atcctggttc taaaggagtt
    26041 caagttacga aaacaactta cgaaacagtg gaaggtgtgg aaacagacaa agtcctttca
    26101 accagtacgg aagtgaccaa ggagcctgta aatcaacagg taatccgtgg tacaaaacca
    26161 attgaaggaa ctcttataga agaaagtctt gaaaagattc catttaagga agttgttaaa
    26221 gaagatgacc aactgaaaaa aggcttagaa gttgtagctc aagaaggaaa agaaggccag
    26281 aaaaaaatca ccaagacctt taataccatt aagggagtta aaacagaaga cgctccaaaa
    26341 gttacagagg aaatccttga ggaacctcaa gacaaaattt tgaaacgtgg taccaagagc
    26401 tttgaaaaac cagtgttgac catcacagaa attgaatcta aggatttgaa gcgtacttca
    26461 gatgttaagt atagtttaga aaatccaagt aaggcagcca ttaaatctat taccctaact
    26521 ctgaaaaaag gtgatgagat tgtcaaaacc ttgaatgttt caccagaaga cttgacaact
    26581 accctgacag atttgcagta ctacaaggat tataaacttg aaacaaagat ggtttatgac
    26641 cgtggtgagg gggatgagga agaagttctg aaagaagaac cgctaagaat tgatctcaaa
    26701 aaagttgaaa taaaagatat tataagaacg gatttaatca aatatgaaaa ccaagttgaa
    26761 actgatgaaa ctcgattgac ttcagtgcca actgatttgt caaattatta tttaaaagtt
    26821 acctctaatg accataagac caatctacta gcggttcaaa aaattgaaga gtcaacaatt
    26881 gatggaaaac aagtctacaa ggtaatagca gtagcggata acttggtcca acgtaatgtc
    26941 gataatcgct ttgaagaaac ttatacctac tatattgaaa aacccaaagc tagtcaggct
    27001 ggtattttct acgactttga agaattgatc caagccattc agacaaatcc tgccggtgaa
    27061 tttaaactgg gtcaaagcat gagcgctcgt aacgttgttc ctaaagggaa atcttatatt
    27121 acttcagagt ttactggtaa actattgagt gatggtgata aacgctatgc tatccataat
    27181 ttggaacatc ctttattcga taagattaag gctggaactg ttaaaaatat aaattttgaa
    27241 aatattgata ttgatttatc taatcaagat aatatcgctg caatagcaaa tgaaatcagt
    27301 ggaacgacac gtatagaaaa tatcaaagca atcggaacca taacagctag aaataatgtt
    27361 gctgggttcg taaataacat aaaagaagac gctactttag aaaatgtggc atttatcggt
    27421 agtataaatt ctgctggtaa taattcaaca gttggaggta ttgccggttc taactatatg
    27481 gggtttgtta atagagccta cgttgatgct actattacag caaataacgc aaatgcaagt
    27541 atgcttgttc ctttcgttca atataaagct catagctggc tatcagggac aaaaggaaaa
    27601 cttacaaact ctgtagcaaa aggtattctt gatgttaaaa atactaaata tgttggtgga
    27661 ataacctcta aaacttggcc atatggagcg gttcaagata atgtaacata tgcgaaagtt
    27721 attaaaggtc aggaaatatt cggttcaaac gatgttaatg atggagatgc aaatcctttt
    27781 gtgtctaatt tatttggagt tattggatat agctcttctg aagatggaac tggtaaagat
    27841 aataaaaaca aaaataaatt aaaacactta tctaaatctg aggctgatgc aaaagttgct
    27901 agtttcaata ttactgctgc taaattcgta agtgaacctt atgaattgaa caaattgaat
    27961 aatgttagca gtcgtgaagc agcttacgag tctattcaag attataatgc agactataaa
    28021 cttgcttata agaatattga aaaactacaa ccattctata acaaagattt tatcgttaat
    28081 caagctaa
//
LOCUS       DAVKGC010000013        26851 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKGC010000013 DAVKGC010000000
VERSION     DAVKGC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26851
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.013"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            22..720
                     /locus_tag="VW049_000473"
     CDS             22..720
                     /locus_tag="VW049_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9431646.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            739..2256
                     /locus_tag="VW049_000474"
     CDS             739..2256
                     /locus_tag="VW049_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9431647.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            2381..3709
                     /locus_tag="VW049_000475"
     CDS             2381..3709
                     /locus_tag="VW049_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9431648.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            3961..4827
                     /locus_tag="VW049_000476"
     CDS             3961..4827
                     /locus_tag="VW049_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9431649.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            4843..5682
                     /locus_tag="VW049_000477"
     CDS             4843..5682
                     /locus_tag="VW049_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9431650.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            5954..6406
                     /locus_tag="VW049_000478"
     CDS             5954..6406
                     /locus_tag="VW049_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9431651.1"
                     /translation="MIFDDLKNITFYKGIYPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYTDLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCYEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            6539..6847
                     /locus_tag="VW049_000479"
     CDS             6539..6847
                     /locus_tag="VW049_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431652.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGAYAAKYGISMARSI
                     LSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAAAWDAYDKIPNNGRINF
                     "
     gene            7134..7778
                     /locus_tag="VW049_000480"
     CDS             7134..7778
                     /locus_tag="VW049_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9431653.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            7947..8864
                     /locus_tag="VW049_000481"
     CDS             7947..8864
                     /locus_tag="VW049_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9431654.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTATHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            8882..9766
                     /locus_tag="VW049_000482"
     CDS             8882..9766
                     /locus_tag="VW049_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9431655.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(9782..10633)
                     /locus_tag="VW049_000483"
     CDS             complement(9782..10633)
                     /locus_tag="VW049_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9431656.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQTSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            10833..12890
                     /gene="pbp2b"
                     /locus_tag="VW049_000484"
     CDS             10833..12890
                     /gene="pbp2b"
                     /locus_tag="VW049_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9431657.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLGSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEINKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            12901..13497
                     /gene="recR"
                     /locus_tag="VW049_000485"
     CDS             12901..13497
                     /gene="recR"
                     /locus_tag="VW049_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9431658.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDSTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            13673..14716
                     /locus_tag="VW049_000486"
     CDS             13673..14716
                     /locus_tag="VW049_000486"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9431659.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDSIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            14800..16173
                     /locus_tag="VW049_000487"
     CDS             14800..16173
                     /locus_tag="VW049_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9431660.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENECK"
     gene            16160..16771
                     /locus_tag="VW049_000488"
     CDS             16160..16771
                     /locus_tag="VW049_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9431661.1"
                     /translation="MNASDFAKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDADEVAELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            16862..17566
                     /locus_tag="VW049_000489"
     CDS             16862..17566
                     /locus_tag="VW049_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9431662.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYRENK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            17785..19158
                     /gene="ftsA"
                     /locus_tag="VW049_000490"
     CDS             17785..19158
                     /gene="ftsA"
                     /locus_tag="VW049_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9431663.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVELVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            19175..20434
                     /gene="ftsZ"
                     /locus_tag="VW049_000491"
     CDS             19175..20434
                     /gene="ftsZ"
                     /locus_tag="VW049_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9431664.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            20439..21110
                     /locus_tag="VW049_000492"
     CDS             20439..21110
                     /locus_tag="VW049_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9431665.1"
                     /translation="MNVKENTELVFREVAEASLSANRERGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            21120..21659
                     /locus_tag="VW049_000493"
     CDS             21120..21659
                     /locus_tag="VW049_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9431666.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            21659..21922
                     /locus_tag="VW049_000494"
     CDS             21659..21922
                     /locus_tag="VW049_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9431667.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            21919..22704
                     /locus_tag="VW049_000495"
     CDS             21919..22704
                     /locus_tag="VW049_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9431668.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            22713..23501
                     /gene="divIVA"
                     /locus_tag="VW049_000496"
     CDS             22713..23501
                     /gene="divIVA"
                     /locus_tag="VW049_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9431669.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLVQLAQCMKNQK"
     gene            23849..26641
                     /locus_tag="VW049_000497"
     CDS             23849..26641
                     /locus_tag="VW049_000497"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9431670.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNSPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGETRKFVVAAELLTSLSEK
                     FGWVDVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDKLRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQVVICDHCASI
                     VEENFVEAVAEGFEEK"
BASE COUNT         8068 a         4898 c         5937 g         7948 t
ORIGIN      
        1 taaagaaaag gaggaaagag gatgccacag attagcaaag aagccttgat tgagcaaatc
       61 aaagatggaa tcatcgtttc ttgtcaggct cttcctcatg aaccgcttta tacagaagcg
      121 ggaggggtga ttcccttgct ggtcaaagcg gctgagcaag gtggagcagt cggtatccga
      181 gcaaacagtg ttcgcgatat caaggaaatt aaggaagtca ctaaacttcc aatcattggg
      241 attatcaaac gtgattatcc acctcaggaa cccttcatca cggctactat gaaagaagtt
      301 gatgaattgg cagaactgga catcgaggtg attgctctgg attgtaccaa gcgtgaacgc
      361 tacgatggtt tggaaattca agagttcatt cgtcaggtta aggagaaata tcctaatcag
      421 cttttgatgg ctgatactag tatcttcgaa gaagggctag cagctgtaga agcaggaatt
      481 gactttgtcg gaacaacctt atcaggctac acatcctaca gtccaaaagt agacggtcca
      541 gattttgaat tgattaagaa actctgtgat gccggtgtag atgtcattgc agaaggaaaa
      601 attcatacac cagaacaagc caaacaaatc cttgaatatg gagtgcgagg catcgttgtt
      661 ggtggcgcca ttactagacc aaaagagatt acagaacgct tcgttgctag tcttaaataa
      721 gatgtgagag ggagttttat gtttaaagtt ttacaaaaag ttggaaaagc ttttatgtta
      781 cctatagcta tacttcctgc agcaggtcta cttttgggga ttggtggtgc actttcaaac
      841 ccaaccacga tagcaactta tccaatacta gacaatagta tttttcaatc aatattccaa
      901 gtaatgagct ctgcaggaga ggttgtattc agtaatttgt cactacttct ctgtgtggga
      961 ttatgtattg gcttagcgaa acgagataaa ggaaccgctg cgttagcagg agtaactggt
     1021 tacttagtta tgactgcaac gatcaaagct ttggtaaaac tttttatggc agaaggatct
     1081 gcaattgata ctggagttat tggagcatta gttgtcggaa tagttgccgt atatttgcac
     1141 aaccgatata acaatattca attaccttcc gctttaggat tctttggagg ttcacgcttc
     1201 gttcctattg ttacatcgtt ctcttctatc ttgattggct ttgtcttctt tgttatttgg
     1261 ccacctttcc aacaacttct tgtttctaca ggtggatata tttctcaggc gggtccaatt
     1321 ggaacttttc tatatggatt tttaatgaga ctttctggag cagtaggctt acatcatata
     1381 atttacccta tgttttggta tactgaactt ggtggtgttg aaactgttgc aggacaaaca
     1441 gtggttggag ctcaaaaaat attttttgct caattagccg atttggccca ttctggatta
     1501 tttacagaag gaacaaggtt ttttgcaggt cgtttctcaa caatgatgtt cggtttaccg
     1561 gctgcctgtt tagcgatgta ccatagtgtt cctaaaaatc gtcgtaaaaa atacgcgggt
     1621 ttgttttttg gagttgcttt aacatctttt attaccggta ttacagaacc aattgaattt
     1681 atgtttctat tcgtcagtcc ggttctatat gttgttcacg cattccttga tggtgttagc
     1741 ttctttattg cagacgtctt aaatatttca ataggaaaca cattttcagg aggtgtaatc
     1801 gatttcactt tatttggaat tttgcagggg aacgctaaga cgaattgggt tcttcagatt
     1861 ccatttggac ttatttggag tgttttgtat tatattattt ttagatggtt cattactcaa
     1921 ttcaacgttc taacgccagg acgaggagaa gaagtagatt ctaaagaaat ttctgaatcc
     1981 gcagattcaa cttcaaatac tgcagattat ttaaaacagg atagcctaca aattatcaga
     2041 gccttgggtg gatcaaataa tatagaagat gtagatgctt gtgtgacacg tttacgtgta
     2101 gctgtaaaag aagttaatca agttgataaa gcacttttaa aacaaattgg tgcagttgat
     2161 gtcttagaag tgaagggtgg cattcaagca atatatggag caaaagcaat cttatataaa
     2221 aatagtatta atgaaatttt aggtgtagat gattaagtac ttactgactt aataaaaaac
     2281 agaggagagt gatggatgag taggatgaaa tgaaatcgca tacaagaaat aaagaactca
     2341 ttatccaagt tggatacgct tattacatag gagaatacaa atgaaattta gaaaattagc
     2401 ttgtacagta cttgcgggtg ctgcggttct tggtcttgct gcttgtggca attctggcgg
     2461 aagtaaagat gctgccaaat caggtggtga cggtgccaaa acagaaatca cttggtgggc
     2521 attcccagta tttacccaag aaaaaactgg tgacggtgtt ggaacttatg aaaaatcaat
     2581 catcgaagcg tttgaaaaag caaacccaga tataaaagtg aaattggaaa ccatcgactt
     2641 caagtcaggt cctgaaaaaa tcacaacagc catcgaagca ggaacagctc cagacgtact
     2701 ctttgatgca ccaggacgta tcatccaata cggtaaaaac ggtaaattgg ctgagttgaa
     2761 cgacctcttc acagatgaat ttgttaaaga tgtcaacaat gaaaacatcg tacaagcaag
     2821 taaagctgga gacaaggctt atatgtatcc gattagttct gccccattct acatggcaat
     2881 gaacaagaaa atgttagaag atgctggagt agcaaacctt gtaaaagaag gttggacaac
     2941 tgatgatttt gaaaaagtat tgaaagcact taaagacaag ggttacacac caggttcatt
     3001 gttcagttct ggtcaagggg gagaccaagg aacacgtgcc tttatctcta acctttatag
     3061 cggttctgta acagatgaaa aagttagcaa atatacaact gatgatccta aattcgtcaa
     3121 aggtcttgaa aaagcaacta gctggattaa agacaatttg atcaataatg gttcacaatt
     3181 tgacggtggg gcagatatcc aaaactttgc caacggtcaa acatcttaca caatcctttg
     3241 ggcaccagct caaaatggta tccaagctaa acttttagaa gcaagtaagg tagaagtggt
     3301 agaagtacca ttcccatcag acgaaggtaa gccagctctt gaataccttg taaacggatt
     3361 tgcagtattc aacaataaag acgacaagaa agtcgctgca tctaagaaat tcatccagtt
     3421 tatcgcagat gacaaagagt ggggacctaa agacgtagtt cgtacaggtg ctttcccagt
     3481 ccgtacttca tttggaaaac tttatgaaga caaacgcatg gaaacaatca gcggctggac
     3541 tcaatactac tcaccatact acaacactat tgatggtttt gctgaaatga gaacactttg
     3601 gttcccaatg ttgcaatctg tatcaaatgg tgacgaaaaa ccagcggatg ctttgaaagc
     3661 cttcactgaa aaagcaaacg aaacaatcaa aaaagctatg aaacaatagt ccttagttat
     3721 tctataaaaa gtagtttttt aaagaaccta agagtgtata cccccttttc cctctacaca
     3781 gatagtgtaa gaaaaggggg cttttgttta aaatgtaaga aactgtcacg aaattaaaat
     3841 gaagttctta cataagcgaa tcataaaaaa tttcattttg attttaaaac agttcaagaa
     3901 agtcaaaaaa ttattctatt tgaaagagag gtgccgactg tgaaagtcaa taaaatccgt
     3961 atgcgggaaa cagtgatttc ctacgctttc ctagcaccag tattattctt ctttgtcatc
     4021 tttgtgttgg ctccgatggt gatgggcttc attacaagtt tctttaacta ctcaatgact
     4081 aaatttgagt ttgtaggctt ggataactat atccgtatgt ttaaagatcc tgtctttaca
     4141 aaatctctga ttaacacagt tattttggtt attggatctg taccagttgt tgttctattc
     4201 tcactctttg tagcatctca gacctatcat caaaatgtca ttgccagatc cttctaccgt
     4261 ttcgtcttct tccttcctgt tgtaacgggt agtgttgccg tgacagttgt ttggaaatgg
     4321 atttatgacc cgctatcagg gattctaaac tttgtcctta agtcaagcca catcatcagc
     4381 caaaacattt cttggttggg agataaaaac tgggcattga tggcgattat gattattctc
     4441 ttgaccactt cagttggtca gcccatcatc ctttatatcg ctgccatggg gaatattgac
     4501 aattcactgg ttgaagcggc gcgtgttgat ggtgcaactg agtttcaagt tttttggaag
     4561 attaaatggc caagccttct tccaacaact ctttatattg caatcatcac aacaattaac
     4621 tcattccagt gtttcgcctt gattcagctt ttgacatctg gtggtccaaa ctactcaaca
     4681 agtaccttga tgtactacct ttacgaaaaa gccttccaat tgacagaata cggctatgcc
     4741 aacacaattg gtgtcttctt ggcagtcatg attgctatcg taagctttgt tcaatttaaa
     4801 gtacttggaa acgacgtaga atactaaaga aaggagacag ctatgcaatc tacagaaaaa
     4861 aaaccattaa cagcctttac tgttatttca acaatcattt tgctcttgtt gactgtgctg
     4921 ttcatctttc cattctactg gattttgaca ggggcattca aatcacaacc tgatacaatt
     4981 gttattcctc ctcagtggtt ccctaaaatg ccaaccatgg aaaacttcca acaactcatg
     5041 gtgcagaacc ctgccttgca atggatgtgg aactcagtat ttatctcatt ggtaaccatg
     5101 ttcttagttt gtgcaacctc atctctagca ggttatgtat tggctaaaaa acgtttctat
     5161 ggtcaacgca ttctatttgc tatctttatc gctgctatgg cgcttccaaa acaagttgtc
     5221 cttgtaccat tggtacgtat cgtcaacttc atgggaatcc acgatactct ctgggcagtt
     5281 atcttgcctt tgattggatg gccattcggt gtcttcctca tgaaacaatt tagtgaaaat
     5341 attcctacag agttgcttga atcagctaaa atcgacggtt gtggtgagat tcgtaccttc
     5401 tggagtgtag ccttcccgat tgtgaaacca gggtttgcag cccttgcaat ctttaccttc
     5461 atcaatactt ggaatgacta cttcatgcaa ttggtaatgt tgacttcacg taacaatttg
     5521 accatctcac ttggggttgc gaccatgcag gctgaaatgg caaccaacta tggtttgatt
     5581 atggcaggag ctgcccttgc tgctgttcca atcgtcacag tcttccttgt cttccaaaaa
     5641 tccttcacac agggtattac tatgggagcg gtcaaaggat aatactctgc gaaaatctct
     5701 tcaaactacg tcagcttcac cttgccatac ttaagtattg cctgcggtta gcttcctagt
     5761 ttgttcttca attttcattg agtataggaa aatcaatcta tcaagataca gaagtatatt
     5821 ttatagattt agagaatata gaggttataa gtgtctacaa aatggagggt atgcagttac
     5881 tttatgaagt tttgtcagac acttatcaac ttaagaatgg ttttagttaa ctatcagaaa
     5941 cgaaggaaag agtatgattt ttgacgattt gaaaaacatc accttttaca aagggattta
     6001 tcctaattta gacaaggcta tcgactatct ctaccaacat cgtaaggatt ctttcgaatt
     6061 aggaaagtat gatattgatg gagataaagt ctttctagtt gttcaggaaa atgtcctcaa
     6121 tcaagctgaa aatgatcaat ttgagtatca taagaactat acagatttgc atttgctgat
     6181 agaagggcat gaatattcga gctacggttc acgtatcaaa gacgaggcag tagcattcga
     6241 cgaagcgagt gacattggct ttgttcattg ttatgaacac tacccactct tattgggtta
     6301 tcacaatttt gcgattttct tcccaggtga gccacatcag ccaaatggtt atgcaggcat
     6361 ggaagaaaag gttcgaaaat atctctttaa aatcttgatt gattaaaaat aggatgaatt
     6421 gtttttttgt aaagctttga taatactcta ccatgaaatt gatctttgtg aggtagagaa
     6481 atgagaataa aatatttaaa aattggtatc ttctaagtat gctgcaagag ctagtttctt
     6541 ggatggacag ggaattacag ttgatgagat ggcttggata attaggggca ttgtgaatgc
     6601 attgattggt agatacataa aattaggtgc ttatgcggct aagtatggta ttagtatggc
     6661 acgctcgatc ttaagtaggg cagctgcaac tgcggcagca agagtaggat tattgaccaa
     6721 gatttttgga tggattttac gaatagctgt gctcgcagct gatgtatatg gtaattttgc
     6781 caacaatatt gctgcagctt gggatgcata tgataaaatt cctaacaatg gtcgtataaa
     6841 cttttaaaat gcgagaatga aagcactttg tattttttta ttgaatatgt tagcttggac
     6901 agtgcttgca atgataattc gtggagggct agatggattt aatgagcata cttggagtac
     6961 tattttaatt gcttcgctgt ccgggatata tatagattat atgtccatag ataaaagtag
     7021 aaaaaagtcc aaaagaaaaa agaaaaaata gatttgttca tggtagggac ttatgaaagt
     7081 tttactgaca aaaaagaaaa cagtttacaa agaaaaatga tggaggagca aacatggcac
     7141 aaaaaggagt aagccttatc aaggcagcat ttgatacaga taactttctc atgcgtttta
     7201 gtgagaaagt cttggacatc gtgacagcca atcttctttt tgtcgtctct tgtttgccca
     7261 tcgtgacgat tggagtggct aaaatcagcc tctacgagac catgttcgaa gttaagaaga
     7321 gcagacgggt gcctgttttt aaaatctatc taagatcttt caagcaaaat ctgaaactag
     7381 gtcttcagct gggtttaatg gagttaggaa ttgtgtttct taccctttca gatctctatc
     7441 ttttctgggg tcaaacagct ctgcccttcc aattgctgaa agccatttgt ttaggtattc
     7501 tgatttttct tactatcgtg atgctggcta gttaccctat cgcggcacgt tatgacctat
     7561 cttggaaaga aattcttcaa aaaggattga tgttggctag ttttaacttt ccttggttct
     7621 tcctcatgtt agccattctt gtcctcattg tgatggttct ttatctgtcc gccttcagtc
     7681 tactcttagg tggctcagtc ttcctacttt ttgggtttgg actattggtc tttatccaga
     7741 ctggattgat ggagaaaatt ttcgcaaaat accaatagga gctttatttc tgaaactact
     7801 ttcaaaggct ccaaacgcta ttctataagc gagaaactaa aatctaagtg tctataagat
     7861 attgaaagcc ttttcggtaa agtgtatact aaagaagtaa agaaaaatct gctttagcag
     7921 aaaataaaaa taataggaga aaatctatgt cagatttgaa aaaatacgaa ggtgtcattc
     7981 cagccttcta cgcatgttat gatgatcaag gagaagtaag cccagaacgt acgcgtgcct
     8041 tggttcaata cttcattgat aaaggtgttc aaggtcttta tgtcaatggt tcttctggtg
     8101 aatgtatcta ccaaagcgtt gaagatcgca agttgatttt ggaagaagtc atggcggtag
     8161 ccaaaggtaa attgaccatt attgcccatg ttgcttgcaa taatactaaa gatagtatgg
     8221 aacttgctcg ccatgctgaa agcttgggag tagatgctat tgcaacgatt ccaccaattt
     8281 acttccgctt gccagaatac tcagttgcta aatactggaa cgatattagt tctgcagctc
     8341 caaatacaga ctacgtgatt tataacattc ctcaattggc aggggttgct ttgactccaa
     8401 gcctttacac tgaaatgttg aaaaatcctc gtgttatcgg tgtgaagaac tcttctatgc
     8461 cagttcaaga tatccaaacc tttgtgagcc ttggtgggga agaccatatc gtctttaacg
     8521 gtccagatga acagttccta ggtggccgcc tcatgggtgc caaagctggt atcggtggta
     8581 cttatggtgc tatgccagaa ctcttcttga aactcaatca gttgattgcg gataaggacc
     8641 tagaaacagc gcgtgaattg cagtatgcta tcaacgctat cattggtaaa ttgactgcta
     8701 cacatggaaa tatgtacggt gtcatcaaag aagttttgaa gatcaatgaa ggcttgaata
     8761 ttggttctgt tcgttcacca ttgacaccag tgactgaaga agatcgtcca gttgtagaag
     8821 cggctgctgc cttgattcgt gaaaccaagg agcgcttcct ctaatctaaa aggaggtatt
     8881 tatgacatac tacgttgcaa ttgatatcgg tggaaccaac atcaagtatg gtttggttga
     8941 tcaagagggg caacttcttg aatcgcatga aatgccaact gaggcgcata agggtggacc
     9001 tcatatctta caaaagacca aagatatcgt agctagttat ttagaaaaag gcccagtagc
     9061 aggtgttgcc atatcttctg ctgggatggt ggatccggat aagggtgaga ttttctatgc
     9121 tgggccgcaa atccctaact acgcaggcac ccagttcaaa aaggaaatcg aagaaagctt
     9181 tactattcct tgtgagattg aaaatgatgt caactgtgca ggtcttgctg aggcagtatc
     9241 tggttcaggc aagggagcaa gtgtgacact ttgcttgacc attggaacag gtatcggtgg
     9301 ttgcttgatt atggatagga aagtcttcca tggttttagc aattcagcct gtgaagtcgg
     9361 gtatatgcat atgcaggatg gagcttttca agacttggct tctacaacag ctttagtgaa
     9421 atatgtagct gaagcccatg gagaagatgt tgatcagtgg aatggccgta gaattttcaa
     9481 agaagccact gaaggaaaca aaatctgcat ggaaggtatt gaccgtatgg ttgactatct
     9541 aggaaaaggt ctggcaaata tttgctacgt tgccaatcca gaagtggtta ttcttggtgg
     9601 tggtatcatg gggcaagagg ctatcctcaa acctaagatc cgcacagcct tgaaagaggc
     9661 tttggtacca agtttagcag aaaaaacacg attagaattt gcccatcacc aaaatacagc
     9721 agggatgttg ggtgcatatt atcattttaa gacaaaacaa tcctagtttg gctcagccaa
     9781 actaggattt tcttacacgt ttttgtctac gatagccgtt gagtttttta ttttcccagt
     9841 agctattaaa gattttttcc ttgctttcgc gattgatttc caaaaagtag gcataaatca
     9901 aatcgataaa gaagagcata ggaagttgag cggatattcg ttggatatag gaggtttggc
     9961 tatgggtggc tacaagaaca gtctctgtat aggtctggct atctttattg ggaacacttg
    10021 taaagagtac agtctttgcc cccatctcct tggcatctaa tagactatct aaaatagaag
    10081 gagttgagcc tgaaagtgag aaacccagta ctagacaatt ttcatccatg atgctggttg
    10141 tccaggcaaa gccgtcttgg tctgtcaaag cttcgcagac cacacctagt cgcataaaac
    10201 gtaatttcat ttcacgggcg acgaggccag aactccctgt tccaaagaag tagatacgct
    10261 cagcatcttc gattagctgg gcaattcgct ctagttggat ttcgtcaatc aagtcttgtg
    10321 tttgttccct catattgcta taacttctga ggactcgttt ggtcagtgga ctatgcttgg
    10381 agacttggtt ggcttgattt tctgcctgat gttggtattg gaaaataaat tctcggtagc
    10441 cagtaaagcc acacttttta gcaaagcggg tcaaagcagc ttgagaaata tgtaattttt
    10501 gggtgacttg ttgagaagat aaatcatctg taatcgtttc agcttgcaaa aaatagcgag
    10561 cgatttcttg ctctaggtct gtcatttctt caaaatgtga atcaatgata gttgcgatat
    10621 ctggtttgtc catagggaaa gctcctttac atgagtcata ctggaagact agatcaggga
    10681 atagtcacac ttcattataa cacataatat aaggatagat aaataaaaac acatctctgt
    10741 tttaaaaacg aaaaaatcga aaaagcttct ttcttttcca taattttcta ctcaaattgt
    10801 ggtacaatta agagtaagat tttaagttag aaatgagact gatttgtatg agaaaattta
    10861 acagccattc gattccgatt cggcttaatt tattgttttc aatcgtcatt ttactcttta
    10921 tgaccattat tggtcgtttg ttgtatatgc aggttttgaa caaggatttt tacgaaaaaa
    10981 agctagcttc agctagtcag accaagatta caagcagttc agcccgtggg gaaatttatg
    11041 atgctagtgg aaaacctttg gtagaaaata cgttaaagca ggttgtttcc tttacgcgta
    11101 gcaataaaat gacggctaca gacttaaaag aaacagctaa aaagttactg acttatgtga
    11161 gcatcagttc tccaaatttg acagaacgcc agctggcgga ttactatttg gctgatcctg
    11221 aaatctataa aaaaacagtg gaagctctcc caagcgagaa acgcttgggt tcagatggca
    11281 atcgtctatc cgaatcagaa ctgtataaca atgcggtcga tagtgtccaa acaagtcaac
    11341 taaactatac agaggatgaa aagaaagaaa tctatctttt tagtcagtta aatgctgttg
    11401 gaaactttgc gacaggaacc attgcgacag atcctctaaa tgattctcag gtggctgtta
    11461 ttgcctctat ttcaaaggag atgcctggca ttagtatttc tacttcttgg gatagaaagg
    11521 ttttggaaac ttccctttct tctatagttg ggagtgtatc cagtgaaaaa gctggtctcc
    11581 cagcggaaga agcagaagcc tatcttaaaa aaggctattc tctaaatgat cgtgtaggaa
    11641 cctcctattt ggaaaagcaa tatgaagaga ccttacaagg aaaacgctcg gtaaaagaaa
    11701 tccatctgga taaatatggc aatatggaaa gcgtggatac aattgaggaa ggtagtaagg
    11761 gaaacaatat caaactgacc attgatttgg cattccaaga tagcgtggat gctttactga
    11821 aaagttattt caattccgag ctagaaaatg gtggagccaa gtattctgaa ggtgtctatg
    11881 cagtcgccct taacccaaaa acaggtgcgg ttttgtctat gtcagggatt aaacatgact
    11941 tgaaaacggg agagttgacg cctgattcct tgggaacggt aaccaatgtc tttgttccag
    12001 gttcggttgt caaggcggcg accatcagct caggttggga aaatggagtc ttgtcaggaa
    12061 accagacctt gacagaccag tccattgtct tccaaggttc agctcccatc aattcttggt
    12121 atactcaggc ttacggttca ttccctatca cagcggtcca agctctggag tattcatcta
    12181 atacctatat ggtccaaaca gccttaggtc ttatggggca aacctatcaa cccaatatgt
    12241 ttgtcggcac cagcaatcta gagtctgcta tggagaaact gcgttcaacc tttggcgaat
    12301 atggcttggg tactgcgaca ggaattgacc taccagatga atctactgga tttgttccca
    12361 aagagtatag ctttgctaat tacattacta atgcctttgg gcagtttgat aactatacgc
    12421 cgatgcagtt ggctcagtat gtagcaacta ttgcaaataa tggtgttcgt gtggctcctc
    12481 gtattgttga aggcatttat ggtaataatg ataagggagg actgggtgac ttgattcagc
    12541 aactgcaacc gacagagata aataaggtca atatatccga ctccgatatg agcatcttgc
    12601 accaaggttt ttatcaggtt gcccatggta ctagtggatt gacaactgga cgtgcctttt
    12661 caaatggtgc cttggtatcc attagcggaa aaacaggtac agccgaaagc tatgtggcag
    12721 atggtcagca agcaaccaat accaatgcgg tggcctatgc cccatctgat aatccccaaa
    12781 tcgctgtcgc agtggtcttt cctcataata ccaatctaac aaatggtgta ggaccttcca
    12841 ttgcgcgtga cattatcaat ctgtatcaaa aataccatcc aatgaactag aaaggaaatt
    12901 atgctttatc caacacctat tgccaagttg attgacagtt attctaagtt accaggtatc
    12961 gggattaaga cggctacgcg tctggccttt tatacgattg ggatgtctgc tgatgatgtc
    13021 aatgaatttg caaaaaatct cctttctgct aagagagaat tgacatattg ttctatttgt
    13081 ggacgtttga cagacgacga tccttgttct atctgtactg attcgactcg tgaccagaca
    13141 acaattttag ttcttgagga tagtagagat gtggcagcca tggaaaatat ccaagaatac
    13201 catggactct atcatgtcct gcatggcctc atttctccta tgaatggtat cagtccggac
    13261 gatatcaatc ttaagagcct tatgactcgt cttatggata gtgaggtttc agaagtgatt
    13321 gtggcgacta atgctacagc ggatggtgaa gcgacctcca tgtatctttc acgtttgctc
    13381 aagccggctg gtatcaaggt tacgcgtcta gcacgaggtc tcgctgtggg agcggacatt
    13441 gagtatgcgg acgaagtgac actcttacga gcgattgaaa atcggacaga gttgtaagtg
    13501 taggcaaatt tacgaactcc attcatttat aaaaaatcaa agaggctgaa aatcgttcct
    13561 atcggcctct ttttgtatag tgtgatgagt agaatcaggt tcaagtttta aaaaaccaag
    13621 caaatatgat atactaaaga gcgagtattc tagtagaatt aggacaaata atatgaaaca
    13681 aacgattatt cttttatatg gtggacggag tgcggaacgc gaagtctctg tcctttcagc
    13741 tgagagtgtc atgcgtgcgg tcaattacga ccgtttcaca gtcaagactt tctttatcag
    13801 tcagtcaggt gactctatca aaacgcagga atttagtcat gctccggggc aagaagaccg
    13861 tctcatgacc aatgaaacca ttgattggga taagaaagtt gcaccaagtg ctatctacga
    13921 agaaggtgca gtggtctttc cagtccttca cgggccaatg ggagaagatg gctctgttca
    13981 aggattcttg gaagttttga aaatgcctta cgttggttgc aacattttgt catcaagtct
    14041 tgccatggat aaaatcacga ctaagcgtgt tctggaatct gctggtattg cccaagttcc
    14101 ttatgtggct atcgttgaag gcgatgatgt gactgctaaa atagctgaag tggaagaaaa
    14161 attggcttat ccagtcttca ctaagccgtc aaacatgggg tctagtgtcg gtatttctaa
    14221 gtctgaaaac caagaagaac tccgtcaagc cttaaaactt gccttccgat atgacagccg
    14281 tgtcttggtt gagcaaggag tgaatgcccg tgaaattgag gttggcctct tgggtaacta
    14341 cgatgtcaag agcacgctac ctggagaagt tgtcaaggac gttgcctttt atgactacga
    14401 tgccaagtat attgataaca aggttactat ggatattcct gccaaaatca gtgatgatgt
    14461 ggtggctgtc atgcgtcaaa atgcagaaac agccttccgt gccattggtg gccttggtct
    14521 atctcgttgc gatttcttct atacagataa gggagagatt tttctcaacg agctcaatac
    14581 catgccaggt ttcacccagt ggtctatgta cccactactt tgggaaaata tggggatcag
    14641 ctacccagaa ctaatcgagc gtttggttga ccttgccaag gaaagttttg acaagcgcga
    14701 agcgcatttg atataaaatg aaagagaggg tagaagccag aaccatcact gcaaggtgac
    14761 tagagttctc ggacttcagc cctttttaaa ggagtagaaa tgaaattaac aatccatgaa
    14821 attgcccaag ttgttggagc caaaaatgat atcagtatct ttgaggacac tcagttagaa
    14881 aaagctgagt ttgatagtcg tttgattgga actggagatt tatttgtgcc acttaaaggt
    14941 gcgcgtgatg gccatgactt tattgaaaca gcctttgaaa atggtgcagc agtaaccttg
    15001 tctgagaaag aggtctcaaa tcatccttac attttagtag atgatgtttt gacagccttt
    15061 caatccctag catcctacta tcttgaaaaa acgactgttg atgtctttgc tgttacaggt
    15121 tcaaatggca agacaacgac taaggatatg ttggcgcatt tactgtcaac aagatacaag
    15181 acctacaaaa cacaaggcaa ttacaataat gagattggcc ttccttacac agttcttcat
    15241 atgcctgaag gaacagaaaa gttggttttg gagatgggac aggatcactt gggagatatt
    15301 catctcttgt ctgaattggc tcgtccaaaa acagccatcg tgaccttggt tggagaagct
    15361 catttggcct ttttcaaaga tcgttctgag attgctaaag gaaaaatgca aattgcagac
    15421 ggaatggctt caggttcctt gcttttagcg ccggctgacc ctatcgtaga ggactatttg
    15481 ccaattgata aaaaggtggt tcgttttggt caaggagcag agctggaaat tactgacttg
    15541 gttgagcgta aggacagtct gacctttaag gctaatttct tggaacaagc ccttgatttg
    15601 ccagtaactg gcaagtacaa tgcgacaaat gctatgattg catcctatgt tgccttgcaa
    15661 gaaggagttt cagaggagca aattcgtttg gcattccaac atcttgaatt gacgcgtaac
    15721 cgtaccgagt ggaagaaagc agccaatgga gcagatatcc tgtcagatgt ttacaatgcc
    15781 aatccaactg ctatgaaact gattttagag actttctctg ccattccagc caatgaaggt
    15841 ggcaagaaaa ttgcagtgtt ggcggatatg aaggagcttg gtgaccagtc tgttcaactt
    15901 cataatcaga tgattttgag cctctcccca gatgtgcttg atatcgttat tttctatgga
    15961 gaagatattg ctcagttggc tcaattggcc agtcaaatgt ttccaatcgg ccacgtttac
    16021 tacttcaaga aaacagaaga ccaggatcaa tttgaagacc tagtcaagca ggttaaggaa
    16081 agccttggag cctatgacca aatcttgcta aaaggttcta actctatgaa tctagccaaa
    16141 ttggtagaaa gtttagaaaa tgaatgcaag tgattttgct aagtatcttc aaagaatgat
    16201 tgctcttaca gatactggac taacctttac aaaggatccg tttgaccgtg agcgctacga
    16261 agacttgcga agtctgttat ctgaaatgtt gaatcaggta tcagacctgg atgcagacga
    16321 agtggcagaa ctcttgaaac cgacctccgc ttatgcgact cctttaatgg acgtccgtgc
    16381 ttggatagtt gaggatgaaa aaatttgtct ggttagggga caaggagaga atgattgggc
    16441 tttgccaggt ggctttggcg aggtcggtta ttcaccaact gaaaatattc tcaaggaaat
    16501 tgaagaagaa accggtttta aagccaaagt tgaacgacta ctagctgttt ttgataccaa
    16561 tcgtttccaa ctacagagca aacaatatgc aaagtttgtt tttgaatgta agcttctcga
    16621 tggacaattt caagaaaatc aagaaatcgc tgaccttcaa ttttttgcaa ttgaccaact
    16681 gccgaattta tctgaaaaac gcattaccaa ggagcaaata gagcttcttt ggcaggttta
    16741 tcaaggtcat agggggcaat atcttgacta agaagatgat tatcgtattt ctaaatccat
    16801 ttttaacaac tagcatggta taataatatg caggaaaatt ttgaattatg aggaagacta
    16861 gatgaattta tgggatattt tctttacgac tcaggcaacc gagccgccca aatttgacct
    16921 tttttggtat gttagcctat ttacgctctt agccttaacc ttttatacag cccatcgcta
    16981 tcgtgaaaat aaggtttacc aacgattttt ccaaatcttg cagactgttc agttaatcct
    17041 tctttatggt tggtactggg tcaatcatat gccactgtca gaaagcctac ccttttacca
    17101 ttgccgtatg gctatgtttg tggtactctt gcttcctggt caatccaaat ataaacaata
    17161 ctttgcatta ttgggaacat ttgggacatt agcagccttt gtttatccag tgccagatgc
    17221 ttaccctttt ccacatatca ccattctatc ctttatcttt ggtcatttag cactcttggg
    17281 gaactctcta gtttatctat tgagacagta taatgcgcga ttgctggatg tgaagggaat
    17341 ttttctcatg acctttgccc taaatgcctt gatttttgtg gtcaatttgg tgacaggtgg
    17401 cgattacgga tttttgacaa aaccgccatt ggttggggat cacggtctag tagctaatta
    17461 tttacttgtt tcaattgtgc tggtagctac tatcagtttg actaagaaaa tcttagaatt
    17521 ctttttagct caagaagcag aaaaaatgat tgcaaaggaa gcttaacaca gagctttctt
    17581 ttttgctctt agagagtttt tacaagcagc ttataaaata agaatttctg aatagacaaa
    17641 ctcaaaaaat ggctgggaaa tttaggaaaa aagcaagcac gattaaattt tttgtgttat
    17701 aatattttgt gaatagctat gcctatgttt agctatggaa taatacgaag tgcgaaactt
    17761 ggaagataga gaggaagcga tgtaatggct agagaaggct tttttacagg tctagatatt
    17821 ggaacaagct ctgtcaaggt gcttgtggcc gagcagagaa atggtgaatt aaatgtaatt
    17881 ggcgtgagta atgccaaaag taaaggtgta aaggatggaa ttattgttga tattgatgca
    17941 gcagcaactg ctatcaagtc agccatttcc caagcggaag aaaaggcagg catttcgatt
    18001 aaatcagtga atgtcggctt gcctggtaat cttttgcagg tagaaccaac tcaggggatg
    18061 attccagtaa catctgatac taaggaaatt acggatcaag atgttgaaaa tgttgtcaaa
    18121 tcagctttga caaagagtat gacacctgac cgtgaagtca ttacctttat tcctgaagaa
    18181 tttattgtgg atggtttcca agggattcgt gacccacgtg gcatgatggg ggttcgcctt
    18241 gaaatgcgtg gtttgcttta tacaggacct cgtactatct tgcacaattt gcgtaagacg
    18301 gttgagcgtg caggtgttca ggttgaaaat gttatcattt caccactagc aatggttcag
    18361 tctgttttga acgaagggga acgtgaattt ggtgctacag tgattgatat gggggcaggt
    18421 caaacgactg tcgctacaat ccgtaatcaa gaactccagt tcacacatat tctccaagaa
    18481 ggtggagatt atgtaactaa agatatctcc aaggttttga aaacctctcg caaattagcg
    18541 gaaggcttga aactgaatta cggggaagcc tatccgcctc ttgcaagcaa agaaaccttc
    18601 caagtagagg ttattggaga agtagaagca gtcgaagtga cggaagccta cttgtcagaa
    18661 attatttctg cacgaatcaa gcacatcctt gaacaaatca agcaagaatt agatagaagg
    18721 cgtctattgg acctccctgg tggtattgtc ttaatcggtg ggaatgccat tttaccaggt
    18781 atggttgagc ttgctcagga agtctttggc gtccgtgtca agctttatgt tccaaatcaa
    18841 gttggtatcc gtaatccagc ctttgcgcat gtgattagtt tatcagaatt tgcgggtcaa
    18901 ttaacagaag ttaatctttt ggctcaggga gcgataaaag gtgagaatga cttaagtcat
    18961 cagccaatta gttttggtgg gatgctgcaa aaaacagctc agtttgtaca atcaacgcct
    19021 gttcaaccag ctcctgctcc agaagtagag ctggtggcgc ctacagaacc aatggcggat
    19081 ttccaacaag cttcacaaaa taaaccgaaa ttagcagatc gtttccgtgg cttgatcgga
    19141 agcatgtttg acgaataaag aggaaaaata aattatgaca ttttcatttg atacagctgc
    19201 tgctcaaggg gcagtgatta aagtaattgg tgtcggtgga ggtggtggca atgccatcaa
    19261 ccgtatggtc gacgaaggtg ttacaggcgt agaatttatc gcagcaaaca cagatgtaca
    19321 agcattgagt agtacaaaag ctgagactgt tattcagttg ggacctaaat tgactcgtgg
    19381 tttgggtgca ggaggtcaac ctgaggttgg tcgtaaagcc gctgaagaaa gcgaagaaac
    19441 actgacggaa gctattagtg gtgccgatat ggtcttcatc actgctggta tgggaggagg
    19501 ctctggaact ggagctgctc ctgttattgc tcgtatcgcc aaagatttag gtgcgcttac
    19561 agttggtgtt gtaacacgtc cctttggttt tgaaggaagt aagcgtggac aatttgctgt
    19621 agaaggaatc aatcaacttc gtgagcatgt agacactcta ttgattatct caaacaacaa
    19681 tttgcttgaa attgttgata agaaaacacc gcttttggag gctcttagcg aagcggataa
    19741 cgttcttcgt caaggtgttc aagggattac cgatttgatt accaatccag gattgattaa
    19801 ccttgacttt gccgatgtga aaacggtaat ggcaaacaaa gggaatgctc ttatgggtat
    19861 tggtatcggt agtggagaag aacgtgtggt agaagcggca cgtaaggcaa tctattcacc
    19921 acttcttgaa acaactattg acggtgctga ggatgttatc gtcaacgtta ctggtggtct
    19981 tgacttaacc ttgattgagg cagaagaggc ttcacaaatt gtgaaccagg cagcaggtca
    20041 aggagtgaac atctggctcg gtacttcaat tgatgaaagt atgcgtgatg aaattcgtgt
    20101 aacagttgtc gcaacgggtg ttcgtcaaga ccgcgtagaa aaggttgtgg ctccacaagc
    20161 tagatctgct actaactacc gtgagacagt gaaaccagct cattcacatg gctttgatcg
    20221 tcattttgat atggcagaaa cagctgaatt gccaaaacaa aatccacgtc gtttggaacc
    20281 aactcaggca tctgcttttg gtgattggga tcttcgccgt gaatcgattg ttcgtacaac
    20341 agattcagtc gtttctccag tcgagcgctt tgaagcccca atttcacaag atgaagatga
    20401 attggataca cctccatttt tcaaaaatcg ttaagtaaat gaatgtaaaa gaaaatacag
    20461 aacttgtttt tcgagaagtt gcagaggcta gtctgagtgc taatcgagag cgtggttcgg
    20521 tctctgtcat tgcagttacc aagtatgtag atgtaccgac agcggaagcc ttgcttccgc
    20581 taggtgttca tcatatcggt gaaaatcgtg tagataagtt tctggaaaaa tatgaagctt
    20641 taaaagatcg agatgtgact tggcatttga ttggtacctt gcaaagacgt aaggtgaaag
    20701 atgtcattca atacgttgat tatttccatg cattggactc agtaaagcta gcaggggaaa
    20761 ttcaaaaaag aagtgaccga gtcatcaagt gtttccttca agtaaatatt tctaaagaag
    20821 aaagcaaaca cggtttttcg agagaggaac tgctggaaat cttgccagag ttagccagac
    20881 tagataagat tgaatatgtt ggtttaatga cgatggcacc ttttgaggct agcagtgagc
    20941 agttgaaaga gattttcaag gcgacccaag atttacaaag agaaattcaa gagaaacaaa
    21001 ttccaaatat gcctatgacc gagttaagta tgggaatgag tcgtgattat aaagaagcga
    21061 ttcaattcgg ttccactttt gttcgtatag gtacatcatt ttttaagtag gagagaacca
    21121 tgtctttaaa agatagattc gatagattta tagattattt tacggaggat gaggattcaa
    21181 gtctccctta tgaaaaaaga gatgagcctg tgtttactcc agtaaattct tcacaggaac
    21241 cggctctccc aatgaatcaa ccttcacagt cggctggtac aaaagagaac aatatcacca
    21301 gacttcatgc aagacaacag gaattggcaa atcagagtca gcgtgcaacg gataaggtca
    21361 ttatagatgt tcgttatcct agaaaatatg aggatgcaac agaaattgtt gatttattgg
    21421 caggaaacga aagtatcttg attgactttc agtatatgac agaggtgcag gctcgtcgtt
    21481 gtttggacta tttggatgga gcttgtcatg ttttagctgg aaatttgaaa aaggtagctt
    21541 ctaccatgta tttgttgaca ccagtgaacg ttattgtaaa tgttgaagat atccgtttac
    21601 cagatgaaga tcaacagggt gagttcggtt ttgatatgaa gcgaaataga gtacgataat
    21661 gattttttta attcgtatga tttataatgc agtggatatt tactccctga ttttggtagc
    21721 cttcgctgtc atgtcttggt ttccaggtgc ctacgaatcc agtttaggtc gttggattgt
    21781 agcgttggtg aaaccagtgc ttgctccctt gcaacgcctg cctttacaga tagcgggtct
    21841 tgatttatct gtttgggttg cgattgtttt ggttcgattt ttaggagaaa acctagtgcg
    21901 ttttctggcg atgataggat gaataaaggg atttatcagc atttctccat agaagatcgt
    21961 ccatttcttg acaagggaat ggaatggata aagaaggtag aagatagcta tgctcctttt
    22021 ttaactcctt ttatcaatcc tcatcaggag aagctattaa agattttggc caaaacctat
    22081 ggtcttgctt gtagcagtag tggggaattc gtctcgagtg agtatgttcg agttttatta
    22141 tacccagatt atttccaacc agagttttca gattttgaaa tatctctcca ggaaattgtg
    22201 tattccaata aatttgaaca tttaacgcat gctaagattt tagggacagt catcaatcaa
    22261 ttagggattg aacggaaact ttttggagat atcctagtag atgaagaacg ggcgcagatt
    22321 atgattaatc agcagtttct tcttctcttt caagatggac taaagaaaat tggtcgtata
    22381 cctgtttcgc tggaggaacg tcctttcacc gagaaaatag ataagctaga acagtatcga
    22441 gaactggatt tatctgtgtc tagttttcga ttagatgttc ttttatcaaa tgttttgaaa
    22501 ctatctagga atcaagcaaa ccagttgatt gaaaagaaac ttgtccaagt aaattatcat
    22561 gtggtagaca aatcagatta cactgttcaa gttggagact tgattagtgt gagaaaattt
    22621 ggtcgcttga gattacttca agataaggga caaacgaaaa aagagaagaa aaaaataacc
    22681 gtccagttat tattaagtaa gtgaggaata gaatgccaat tacatcatta gaaataaagg
    22741 acaagacttt tggaactcga ttcagaggtt ttgatccaga agaagtcgat gaatttttag
    22801 atattgtggt tcgtgattac gaagatcttg tgcgtgcgaa tcatgataaa aatttgcgta
    22861 ttaagagttt agaagagcgt ttgtcttact ttgatgaaat aaaagattca ttgagccagt
    22921 ctgtattgat tgcccaggat acagctgagc gagtgaaaca ggcggcgcat gaacgttcaa
    22981 acaatatcat tcatcaagca gagcaagatg cgcaacgctt gttggaagaa gctaaatata
    23041 aggcaaacga gattcttcgt caagcaactg ataatgctaa gaaagtcgct gttgaaacag
    23101 aagaattgaa gaacaagagc cgtgtcttcc accaacgtct caaatctaca attgagagtc
    23161 agttggctat tgttgaatct tcagattggg aagatattct ccgtccaaca gctacttatc
    23221 ttcaaaccag tgatgaagcc tttaaagaag tggttagcga agtacttgga gaaccgattc
    23281 cagctccaat tgaagaagaa ccaattgata tgacacgtca gttctctcaa gcagaaatgg
    23341 aagaattaca agctcgtatt gaggtagccg ataaagaatt gtctgaattt gaagctcaga
    23401 ttaaacagga agtggaaact ccaactcctg tagtgagtcc tcaagttgaa gaagagcctc
    23461 tgctcgtcca gttggcccaa tgtatgaaga accagaagta gctccagtgc atccgacagg
    23521 tccaacacca gctacagaaa ctgttgattc agcaccggga tttgaagcac cgcaagaatc
    23581 tgttacaatt ttataagaaa tattctgaga acaatatctt atccttatat tcccagcgag
    23641 caggagatgg tgtgagtcct gcattcccta tcgataagat tatcctctca aactatcaag
    23701 tctgaatcta gtaagatttg acgttcccca cgttacggga taagagggag aaagactaaa
    23761 actttttccg aataaaggtg gtaccacgat tttcgtcctt tttggaagtc gtggttttta
    23821 atttgttatt atttataaag gagataccat gaaactaaaa gataccctta atcttgggaa
    23881 aacagctttc ccaatgcgtg caggccttcc gaccaaagag ccagtttggc aaaaggaatg
    23941 ggaagatgca aaactttacc aacgtcgtca agaactaaat gaagggaaac cgcattttgt
    24001 cttgcatgat ggccctccat acgcaaatgg aaatatccac gttggacatg ccatgaatca
    24061 tatctcaaaa gatatcatca ttcgttctaa gtccatgtca ggttttaact caccttatat
    24121 cccaggttgg gatactcatg gtctgccaat cgagcaagtt ttggcaaagc aaggtgttaa
    24181 gcgcaaagaa atggatttgg ttgagtactt gaaactttgc cgtgaatacg ctctttctca
    24241 agtttataaa caacgtgatg atttcaagcg cttggggatg tcaggtgact gggaaaattt
    24301 atatgtgact ttaactccag actacgaagc agcccaaatc cgtgtatttg gtgagatggc
    24361 taataagggt tatatctacc gtggtgctaa gccagtttac tggtcttggt catctgagtc
    24421 agcccttgcc gaagcggaaa ttgaatacca tgatttggtt tcaacttccc tttactatgc
    24481 caataaggta aaagatggca aaggtgttct agatacagat acttatatcg ttgtttggac
    24541 aactactcca tttactatca cagcttctcg tggtttgact gttggtgcag atattgatta
    24601 cgttttggtt caacctgctg gtgaaactcg taagtttgtc gttgctgcag aattgttgac
    24661 tagcttgtct gagaaatttg gctgggttga tgttcaagtt ttggcaactt accgtggtca
    24721 agaactcaac cacatcgtaa cagaacatcc atgggataca gctgtagatg aattggttat
    24781 ccttggtgac cacgttacaa ctgactctgg tacaggtatt gtccatacag cccctggttt
    24841 tggtgaggac gattacaatg ttggtattgc taataatctt gaagtcgcag tgactgttga
    24901 tgaacgtggt atcatgatga agaatgctgg tcctgaattt gaaggtcaat tctatgaaaa
    24961 ggtagttcca actgttattg aaaaacttgg taacctcctt cttgcccaag aagaaatctc
    25021 tcactcatat ccatttgact ggcgtaccaa gaaaccaatt atctggcgtg cagtaccaca
    25081 gtggttcgcc tctgtttcaa aattccgcca agaaatcttg gatgctattg ataaagttaa
    25141 attccataca gaatggggta aagtacgtct atataatatg attcgtgacc gtggtgactg
    25201 ggttatctct cgtcaacgtg cttggggtgt tccacttcca atcttctatg cagaagacgg
    25261 tacagctatc atgacagctg agactattga acatgtggcg caactctttg aagtacatgg
    25321 ttcaagcatt tggtgggaac gtgatgccaa ggacctcttg ccagaaggat ttacacatcc
    25381 aggttctcca aatggggagt tcaagaaaga aacagatatt atggatgtat ggtttgactc
    25441 aggttcatca tggaatggtg ttttggtaaa tcgtccaaat ctgacttatc cagctgacct
    25501 ttaccttgaa ggttctgacc aataccgtgg ttggttcaac tcatcactca ttacatctgt
    25561 tgccaaccat ggcgtagcac cttacaaaca aatcttgtca caaggttttg cccttgatgg
    25621 taaaggtgag aagatgtcta aatctcttgg aaataccatt gctccaagcg atgttgaaaa
    25681 acaattcggt gctgaaatct tgcgtctctg ggtaacaagt gttgactcaa gcaatgacgt
    25741 gcgtatctct atggatattt tgagccaagt gtctgaaact taccgtaaga ttcgtaacac
    25801 tcttcgtttc ttgattgcca atacatctga ctttaaccca gctcaagatg tagtcgctta
    25861 cgataaactt cgttcagttg ataagtacat gactattcgc tttaaccagc ttgtcaagac
    25921 cattcgtgat gcctatgcgg actttgaatt cttgacgatc tacaaggcct tggtgaactt
    25981 tatcaacgtt gacttgtcag ccttctacct tgattttgcc aaagatgttg tttacattga
    26041 aggtgccaaa tcactggaac gccgtcaaat gcagactgtc ttctatgaca ttcttgtcaa
    26101 aatcaccaaa ctcttgacac caatccttcc tcacactgcg gaagaaatct ggtcatatct
    26161 tgagtttgaa acagaagact tcgtccaatt gtcagaatta ccagaagttc aaacttttgc
    26221 taaccaagaa gaaatcttgg atacatgggc agccttcatg gactttcgtg gacaagcaca
    26281 aaaagccttg gaagaagctc gtaatgcaaa agttatcggt aaatcacttg aagcacactt
    26341 gacagtttat ccaaatgaag ttgtgaaaac tctactcgaa gcagtaaaca gcaatgtagc
    26401 acaacttttg atcgtgtctg agttgaccat cgcagaagga ccagctccag aagctgccct
    26461 tagcttcgaa gatgtagcct tcacagttga acgtgctgct ggtgaagtat gtgaccgttg
    26521 ccgtcgtatt gacccaacaa cagcagaacg cagctaccag gtagttatct gtgaccactg
    26581 tgcaagcatc gtagaagaaa actttgtgga agcagtcgca gaaggatttg aagagaaata
    26641 agattgaaaa gtctaggcaa aattcaattt gagaagaaaa gacaactaat tttatagtct
    26701 attaaacgca ttgtatcacg tttttgaaca cctgatacga tgcgtttttt atttatttta
    26761 aaaatttgcg aggtatgact ttttatactc aacaagaatc aaagagaaac ttagcaagct
    26821 aacagtagta agataaaata ggaatttgat a
//
LOCUS       DAVKGC010000014        26027 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKGC010000014 DAVKGC010000000
VERSION     DAVKGC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.014"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(1..330)
                     /locus_tag="VW049_000498"
                     /pseudo
     CDS             complement(1..330)
                     /locus_tag="VW049_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            818..937
                     /locus_tag="VW049_000499"
     CDS             818..937
                     /locus_tag="VW049_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9431671.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            complement(1033..2379)
                     /gene="glnA"
                     /locus_tag="VW049_000500"
                     /pseudo
     CDS             complement(1033..2379)
                     /gene="glnA"
                     /locus_tag="VW049_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
     gene            complement(2416..2772)
                     /gene="glnR"
                     /locus_tag="VW049_000501"
     CDS             complement(2416..2772)
                     /gene="glnR"
                     /locus_tag="VW049_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9431672.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYENQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            complement(2849..3376)
                     /locus_tag="VW049_000502"
     CDS             complement(2849..3376)
                     /locus_tag="VW049_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9431673.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            complement(3512..4708)
                     /locus_tag="VW049_000503"
     CDS             complement(3512..4708)
                     /locus_tag="VW049_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9431674.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(4798..9777)
                     /locus_tag="VW049_000504"
     CDS             complement(4798..9777)
                     /locus_tag="VW049_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU9431675.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVATASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNIGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTVQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTSDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDKGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEGLTTAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELTFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNKVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVYENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            10070..11701
                     /locus_tag="VW049_000505"
     CDS             10070..11701
                     /locus_tag="VW049_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU9431676.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQATGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            complement(12134..13741)
                     /locus_tag="VW049_000506"
     CDS             complement(12134..13741)
                     /locus_tag="VW049_000506"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9431677.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(14131..14718)
                     /locus_tag="VW049_000507"
     CDS             complement(14131..14718)
                     /locus_tag="VW049_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9431678.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(14846..15295)
                     /locus_tag="VW049_000508"
     CDS             complement(14846..15295)
                     /locus_tag="VW049_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9431679.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNYASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(15300..15689)
                     /locus_tag="VW049_000509"
     CDS             complement(15300..15689)
                     /locus_tag="VW049_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU9431680.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(15717..16343)
                     /locus_tag="VW049_000510"
     CDS             complement(15717..16343)
                     /locus_tag="VW049_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9431681.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKTPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(16357..16920)
                     /locus_tag="VW049_000511"
     CDS             complement(16357..16920)
                     /locus_tag="VW049_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9431682.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(17085..17319)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(17373..17876)
                     /locus_tag="VW049_000512"
     CDS             complement(17373..17876)
                     /locus_tag="VW049_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9431683.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(18069..18899)
                     /locus_tag="VW049_000513"
     CDS             complement(18069..18899)
                     /locus_tag="VW049_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9431684.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATIFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(18884..20566)
                     /locus_tag="VW049_000514"
     CDS             complement(18884..20566)
                     /locus_tag="VW049_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431685.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLISLLNQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHCPVDRIV
                     LINDGRILFNGSTDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLYIS
                     KGQVQLRTELVKETPELQSLFRLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKALSSFIQTEGRYLWEGQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEQEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQQGHTIVMITHDMQLMLDYSDRALVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(20668..20925)
                     /locus_tag="VW049_000515"
     CDS             complement(20668..20925)
                     /locus_tag="VW049_000515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431686.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(20932..21480)
                     /locus_tag="VW049_000516"
     CDS             complement(20932..21480)
                     /locus_tag="VW049_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9431687.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(21495..22343)
                     /locus_tag="VW049_000517"
     CDS             complement(21495..22343)
                     /locus_tag="VW049_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9431688.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     DQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            22531..23880
                     /gene="trkA"
                     /locus_tag="VW049_000518"
     CDS             22531..23880
                     /gene="trkA"
                     /locus_tag="VW049_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9431689.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            23884..25323
                     /locus_tag="VW049_000519"
     CDS             23884..25323
                     /locus_tag="VW049_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9431690.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKMVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(25448..25705)
                     /locus_tag="VW049_000520"
     CDS             complement(25448..25705)
                     /locus_tag="VW049_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEU9431691.1"
                     /translation="MNEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYRE
                     MKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
BASE COUNT         7497 a         5790 c         4786 g         7954 t
ORIGIN      
        1 agctatcaaa tactctttaa gttctctaac tacagcaata ttttttagat atggtctaac
       61 tgttgaaaat aagacactat tctgcgaaac taattttcta gcacgggaag gcgcttgttc
      121 aggtgaaaga tattgtagat ttttgtagtt gattatgttc ttttttctat caatactaga
      181 cgtatctata tacctaaagg atttctctgg cttattttgc ccaaaattcc aataaattga
      241 ttttatcctc acccactccc aagtatcagg aatatcataa ggaacatcaa tttcttgagt
      301 acttccatca gcaaacttcc cataatgttt cttatgtgct tcaagtatat aaaaaggcgt
      361 aaaaatacgc ctatagataa tggggttgaa ataggtttat tgttgatgag attgtagata
      421 attcaatttt ttacttccaa tcgaatattc aaaacctcca ccttttctgc ctgtaattgt
      481 tcatcataaa attcaatatc ttcaggattt tccccttgac aacctcggca gaaatattct
      541 tccgctcgat caggattcaa aaatcgacaa gcacaaacaa aacagtcgcc atcatcattt
      601 attgagataa tatagtagat tgaaataaga tgtaaacaaa tcgattagga aagttaaatt
      661 aatttctaga aatttttagc agatgtagtg tactattcta gtctcaattt actatggctt
      721 caaatatatc tttcgaaaaa atatttacag atgtgtaatt ttgaagcttg caaaagttag
      781 taaacttgta gatttcgatt tgaagtaact tgttttcttg cccgatattg tttttgaaat
      841 tgaatttttc catagtgact ccttgatttt cttctacacg tctgatgata aatctaattc
      901 gcaaaagagt caagaggatt tttcgaaaaa taaatagcga ccgaaatcgc tattttaagg
      961 gttataggta tttgatggct tagactgctg tgtgactgtt tacccacagg caatctttct
     1021 tctatattag tattagtaaa ggtctaaata attatcaatt tcccattgtg aaacgaaggt
     1081 tgcataactt gcccattcga ttcgtttggc ttcaaggaag ctagtgtaga tgtgatctcc
     1141 gagagcagct ttaaccactt catcttctgt caaagctttc aaagcgttgt gaagagttga
     1201 tggaaggtct gtaataccag cttccttgcg ctcttctgct gtcatgatgt agatattttc
     1261 ttcgatagga gctggtgctt cgattttatt ttcaatacca tacaaaccaa cttccaaaag
     1321 aacagccata gcaatgtaag ggttcgccat tggatccact gaacgcaact caagacgagt
     1381 tcccatacca cgtgaagcag gtacgcgcac aagtggcgaa cggttacgac cagcccaagc
     1441 aatgtaaaca ggcgcttcat aacctggaac caaacgtttg tatgagttaa ctgttgggtt
     1501 catgatgaca gtatagttgt aagcatgctt gatcaaaccg cctaggaaat ggtaagctgt
     1561 ttctgacaac tgcattcctt ttggatcatt tggatcaaag aaggcgttat ttccttctgc
     1621 atcaaacaag gacatattac agtgcatacc tgagccagca ataccaaatt ttggcttcgc
     1681 cataaatgtt gcgtaaagtc cgtgtttgcg agcaatggtt ttaacaacaa gtttaaagat
     1741 ttgaatctta tcacaagcac ggagaacttc atcgtactta aagtcaatct catgctgtcc
     1801 aaccgcaacc tcgtggtgac tcgcttctac ttcaaatccc attttggtca agacattcac
     1861 aatctaacga cgtgtgttgt ccgcaaggtc agtaggtgcc aaatcaaagt agccaccctt
     1921 gtcattcact tcaagtgttg ggtccccatt ttcatccaac ttaaatagga agaattctgg
     1981 ctctggacca aggttgaagg atttgaatcc aacttcttcc atgtgacgaa gagctcgttt
     2041 caaattacca cgagggtcac ccgcaaatgg ttcaccttct gttgtataaa catcacagat
     2101 cagacctgca acacttccat tttcatctcc ccaagggaag actgtccatg tatccaagtc
     2161 cgggtacaag tacatatccg actcattgat acgtacaaaa ccttcaatag aagatccatc
     2221 aaacataacc ttgttcgaca agaccttatc taactgttca tctgtagcag gaatttcgac
     2281 gtttttcatg gttcccaaaa tatctgagaa cataagacga ataaaggtaa catttttttc
     2341 cttgacttca cgacgaatat ctgcagctgt gattggcata agttttctcc ttaatctatg
     2401 actacttgcg gttgcctaac cgcgaccaaa aggtgactgt actgaagcaa aacgcccctg
     2461 ttggaggagt tcattgtgaa gtgcacgacg tacttcagtc tgactaaccg ctttcttgga
     2521 tttcgcttca cgttcagcat attttttctt aatggcagcg atattataac cttcagagat
     2581 ataatctttg atttcaagca gacgatccat gtcattcaag gaatacatgc gacgatttcc
     2641 ttcgtttcga tcgggcttga tcaactcttg attttcataa taacgaatct gacgcgccga
     2701 tagatcggtc aacttcataa cactgccgat aggaaaaaca gccatatttc ggcgaaattc
     2761 tttttccttc atttacaatt tccttctttc tgtctattat agtctaaaaa aagacaaacg
     2821 tcaattaata atgttataaa atgtaacatt atttttcttt tttctctaaa aagagacgaa
     2881 tacgatcaat atcgtaattt acgataattg cgacaaaaac tcccataaat gtttctaata
     2941 cacgcacaaa cacgtacaaa attgtctcac cacttggaat tgatagggta ataattaaca
     3001 tagctgctac accaccaata acccctgctt tgttattcat ggctacattt gtcataatgg
     3061 ttaacatggt gcagattgga acaactacca aggtcaccca aaaggcttcg tggaaaaagg
     3121 tatttaataa gaagaagacc aaggcataga gtccaccgat actatttcct agaatacgcg
     3181 aagtcccaaa atgaacactc tcatcaaaac tctccctcag gctaaaaacg gctgtcaaag
     3241 caccaatttg aagacctttc cagccaaaaa agccaaaaat caagagaact agaaaaacag
     3301 caatacctgt tttaaaggtt cgcataccaa gtttgaactg ggatttatcg aatttatatt
     3361 ttttaaaata actcataatc tcaactttct atttccattt tatcataaat cggtgatttt
     3421 tatgagtaat agttgagagg aagcgttttt attttaagca aaagaaaaga ggaactttca
     3481 tccctctctt ctttgattta tttataaaat cttatttttc tgtcaaggct gcaagtcctg
     3541 gaagaacctt accttcaaga agttccattg atgctccacc acccgtacta atccatgaga
     3601 acttgtctgc acggccaagg ttaatcgctg cggcagctga gtcaccacca ccgatgattg
     3661 atttaactcc tggttgtttc acgatagcgt ccatcacacc gattgtacca gcttggaaat
     3721 ctgggttttc aaatacaccc ataggtccgt tccatacaac tgttttggca ccagtcaaag
     3781 cttcgtcaaa tttggcgata gattttggac cgatgtcaag accaaggaag ccttcagaaa
     3841 ctgcttcacc ttcagtgtca cgcacttcag tgtaaccagc aaatgcgtta gcttcttttg
     3901 agtcaactgg caagatcaat ttaccatttg ctttttcaag aagagctttc gcaacatcca
     3961 atttgtcttc ttctacaagt gagttaccga tttcgatacc ttgtgctttg tagaatgtgt
     4021 aagtcatccc accaccgata aggactttat cagctttttc aagcaagttt tcgataacac
     4081 cgatcttgtc tgaaactttt gaaccaccaa ggatagccac gaatggacgt tctggagttt
     4141 caactgcttc ttggatgtag gcaatttcgt tttcaagaag gaaaccagca actgcttttt
     4201 caacgtttgc tgagatacca acgttagatg cgtgtgcacg gtgagctgta ccgaatgcat
     4261 cgtttacgaa gataccatct ccaagtgatg cccagtattt accaagttca ggatcgtttt
     4321 tagattcttt cttgccgtca acatcttcgt aacgagtgtt ttcaaccaag agaacttgtc
     4381 catcttcaag agcgttgatt gccgcttcca attcagcacc acgagtgaca cctgggaaaa
     4441 caacatcttg accaagtttt gctgccaagt cagctgctac aggagcaagt gatttaccag
     4501 ctttatcagc ttcttctttc acacgtccaa ggtgagagaa aagaattgca cgtccacctt
     4561 gttcgatgat gtacttaata gttggaagag ctgctgtgat acggttatcg ttagtgatta
     4621 cgccatcttt caatggtacg ttgaagtcaa cacgaacgag gactttttta cctttcaagt
     4681 caacgtcttt aacagtaagt tttgccatgt tacaaaaact cctttattta ttttattcga
     4741 aactattata tcacactttg gaaatataag gaaagatttc acaagatttt tcgatattta
     4801 atctttatct tctcttttcc gtctcaaggt caaggctaaa ctagcaagac caaggctggc
     4861 taagcctgct attagggctt cattagcatc agctgttcct gtatttggca attgtttgct
     4921 tgcttcttct gatttaactg ctgtattttg agctggtttt tcctgtgtag ctactactgc
     4981 tggtactgtt tttacttttg ttccaatttc aacaatttct ggaatcgctt cttggatgac
     5041 ttctgttgaa cgaagacgac gctgaccgtt ttcataaact tcaaagacat gtcttaacag
     5101 tccatctcgt ccctgtatga tgactcgttg ttccccaact agcatctcag cattttcatg
     5161 ctcttgacga tgaaaggcaa ccttttcctc ttgaacttcc aatttaggag ccttttgttg
     5221 atcagaagcc ggttgataag taggttttgg aaggactttg ttttgtggtg ctggtttagc
     5281 aggttgtggt ttttcagcag attcataaac aaaggcataa tgagtaaagt gaggtgctgt
     5341 aaagataaca tgactatccg tttgttcaaa agtcaattct actgcctctt tgccttcagg
     5401 taagaaaaat actttcttaa ctttcttatc tttttcaatt gggattttca caatagaagc
     5461 ataagagaga tcaacatctt gacctttttc atccaaacct tctatttcaa agacatgagc
     5521 atcttctcca gcaaagtatt tcttctcttc agcacttgct tgaacacgct ctactttcaa
     5581 acccttgatg acagtcttct ctttatttga gaagtgtact tcaacacctg ttttactatc
     5641 agaaaagact gctggcttgt cttctttcaa taaagcaacc agatctttta gactctgttt
     5701 cgctgttgta agcccttctg gactcaaatc tactttttca accaaggcct tgtttttctc
     5761 aagaagcgga gctagagcag aacctgaagg atggtttggt ttggcagcca agagttcttg
     5821 aacctcacct gttaaacgat caagctctgt cttttcttcc gtaaatttct cttgaccatt
     5881 cagagcttta tgagcttctg tcaatttatt aagtcggtca ttgacttgat cttgggtgct
     5941 agtttcatgg tctttcaaca cttgttcggc cgcttcaatt gctttcaaga aagcttcttt
     6001 tacttctggt gaagcaaagt tatagaccac cttgttctta gcagcttcaa cttctgctaa
     6061 tttctgtttg aggtactcat cgttcaatgc tgcttttgga gataccaaaa catcaaagtt
     6121 gacttcatga ccttggtaac gaagcgtcaa ggtttgacga ccagtctttt gagcatcata
     6181 accagaaatt tcaactccct tatcagtaaa ggaatgttct tccatggtgt cattgctata
     6241 agccactgca aagcgtcctt cagacaagtc taagctatca ccaactaggt aatctttttt
     6301 cggttcctga cttacttcaa ttcccaaaat agttttcgga cttgcttcgt cttggccagt
     6361 gacagtcact gacaaattag catttaccgg ttgtcccaaa tattggagag taagattctg
     6421 ttctccctta tgatgcgtat caaaacctga tactgataca cctgcgtgag ttaggcgaat
     6481 gagttcgtcc tcagttcctc cttcatactg aactcgaaga acaccacctc taaggtccaa
     6541 atcctcccct tcggcgtagc ttgttttctt aggtcctgtt tctaggctga ctgacttgat
     6601 tcttctgtca tcttttggaa cggaaactgc taggacatta ctaatttcct tgcctggcaa
     6661 ctgggtacga taataaagaa gaccagattg atttgtcaaa tccaatggtg cacttgctag
     6721 gtctcctcca acttcgctct tcaaggttgc gagtggagtt tgagaatttg gattatcata
     6781 aacggtaata gttgctccag ccggtacatc tgcaaagcca acttgtacct tgttattgcc
     6841 tagagaacgg gctgcagcct tggccatcgg aatattgaca ctctcagtat caagcttttc
     6901 atacattttc cagttataga tacgaatagc cttccatgga gttccattgt cagaagtgac
     6961 aacattcaag cgccagtctt gagcagtgat tggtttatca agagtgatat ctgtcacgtg
     7021 tgctttgttg ccacggactt ccttggctag cttccactca ccatctgcat ctttataata
     7081 aaggtcaaag tctttggtgt tcatcaagcc atcgttaaca gactcaccac cagcccctgc
     7141 atgatccatg acccatctaa caacggtacg tggcttggtc aaacgaatat ccacactacc
     7201 actcaactga gctgaagacc atttatctga caagctagta atggtaccgt tcaacatacc
     7261 ttcaatacct tctccacctt cagtcttagg gaaagtacta tcaataactg ttgcacctgg
     7321 aacgatattt tcagctagtg gttttggtag gctggtatct tttacagtca taccccaatc
     7381 aaaggttgtg gttgcagctt ctgaacgaac tccattttta ccgactgcta caaccttcag
     7441 ttcttgagtt gtaccctgag cacttgctga gcggctaact tttggtagat aaatagttgt
     7501 agaagatgag ccagttagta atttccagct gtctccatct ttttcataaa cttcatagaa
     7561 atctgcatcc ttgttgcctt taaattgcac aactgcttcc gcttcttggg catttttaag
     7621 agattgtttc actacagaaa agcttgtcgg cgattgtggc tcttggtgat tgtccgagat
     7681 agttaattgt ccgaggttga actgataatc ttttacagca ccttcatgct cgaaaaatag
     7741 tttgactgca tagatggttt tacccgctag tgagctgaga tcaaattctt catttgtcca
     7801 gttgtcagaa agggttagct ctttccatgc atctgcatca tcgaatttgt agtctgaagt
     7861 tgtagagaat gccatataaa ctttagaacc ttttcctccc ttgtgggcaa cacgaagttt
     7921 ggtcttctca gttacttcta acttagtaga ataaagtctc acatcctgaa ctgtcttacc
     7981 ggctacatca ccagagaatt taagggaatt tccgccatta taggcatctg taaaatcata
     8041 ttctgcacga agtttttccc ctgttgaagt ctgccaccag cgccatgttg gaagaacacc
     8101 tgatactgaa cgataattcc actcagaatc cttagaaacc ttaccatcta cgaaccattt
     8161 tttaccatga cctatattaa aagaagtagt aaaagtatta cctaccgctg gcgtacggtc
     8221 cgcaactagg ttagcaatac cataccaatc tttgtcacct ggtttttggc cagtggggtc
     8281 tccttgataa cctgtaaaga agatatcttc atttttatga taatcttcac cagtttttcc
     8341 taaacttgta atggtatctg gggcaaataa accaagagaa aggcgcaatt tcccattttc
     8401 gtctaaaatg tcattccact taacctttgt cttgtaggaa ccaccctgtt gcaattccaa
     8461 acctgcaaat acatcataag gattacgacc aatccagttg gcagttgcaa tagtgtaatc
     8521 atttttagcc ttatcccagt taaagttagc aaagaagtta tctgccggaa ccttatctcc
     8581 ttctggttgc atgaattggt agttgtattc tcccaaacca tcttgatggt aacgtccata
     8641 gttataggtc atggcatcat accaagaata cttgattgga tggtttacct tagcagcata
     8701 ttccttgcta tagagcataa actggcgcat cttttctcca agaggtttaa ccaaatctcc
     8761 agttgtttct tggttgatga aatagccatc atagccataa tactttgcca tgtctaccaa
     8821 tttacgagca attgggaagc taccatctgc gtcttgcttc aaagcttcag caaatctttc
     8881 ttgatctgca atactattag accagttgaa gaagagtgta ccgtatacag gaaccccgtt
     8941 acggtgacct gcatcaataa cgtcaggagt tggtacgaga ccttcccaga agaccattga
     9001 atctagatat tgccaatagt caaaagcata ggccttgaac tcttctccac caacagaagc
     9061 atggtctttt gctttagaat tagtgtttga taaggcttga acttttgctt ccttgctagc
     9121 tttttcattg actaaatgac ctgtccgacg tgaagcgagg acaacagatc cgcggttaat
     9181 agcatcatct tcacgagcac ctggttccca ttttaacaat tcttcccaag aattaaattt
     9241 gatttccttt ggttttaatg ttttttctgt atttttaggg acatctggct ggacttcttt
     9301 ttcagtctta ttagcttcgg ctgctggctt agactcatcc ccttggttag ttgcttcggg
     9361 ctttgcttct gtttccttgt cagacttagg ttttgcttct gcagatggtg tttctgcaac
     9421 cacctcactt gctttttcct ctggtttagc tgcgctagct gcttctgcag tttcagtagt
     9481 tgcagcttca ttgcttgctg gcgaagcagt ttcagatgct gtcgctactc cttctgcctt
     9541 ttctagcttg ttttctctag catgttcccc ttcattctgc ttatcagctt cattggtcga
     9601 atgctcactc tctcctgaaa caagctctgt attagctccg ctattttcag gaactgcagt
     9661 ttcttcagcc aaggctggac cagcaaataa aacagcacca atcatcagcg agcaggctcc
     9721 tactgataac ttacgaatac tgtaacgaca acgtctttca aaaaatggat tcttcatttt
     9781 ctcctcctaa aagattgatt ttgttgtttt caagaaagcg ctttatattc tgtagttttt
     9841 tatcaaactc tagaaccatg ataatcacat ttttatccta aaacattttt agacaaaaaa
     9901 caataactgt ttgcacaaat ctaaaataag gttaaaaact cttaaaaata atacacttat
     9961 caattataat tttcttttac aaacttgaca ttaacacttg ttagatttac ttttatcctt
    10021 taaaatagaa taggagatat tccgcgatta tttttcggag gaaaaagaaa tgtccattaa
    10081 ttggcaggaa attttatttc actttttagg tggtctgggg ctattcttat atagcatcaa
    10141 gaccatggga gacggtttac aacaagctac tggagatcgc cttcgttttt acattgacaa
    10201 atatactagt aatcctttct ttggagttct ggttggtatt gggatgactg ctctaattca
    10261 gtctagttct ggtgtaacag ttatcacagt cggcctggtc agtgccggtc tcttaacctt
    10321 acgtcaggct atcgggattg tcatgggtgc taatattggg acaactgtca catcctttct
    10381 catcggtttt aaattaggta actatgccct acctatgctc tttatcggtg ccgtctgtct
    10441 cttttttaca aaaaatcgga cagtcaataa tatcggacgc atcctctttg gtgtcggtgg
    10501 tatctttttt gccctcaatc tcatgagcgg cgcaatggct ccactcaagg atttacaggt
    10561 ctttaaggac tatatgattg agctaagtaa gaatcctgtt ttgggtgtct ttgtcggtac
    10621 tggcttgacc ttgctaattc aagcttcttc ggctaccatt gggattttac aaaacctcta
    10681 cgccggcaat ctaattgacc tacagggagc tttgccagtt ctatttggtg acaatatcgg
    10741 gacaaccatt acagccatca ttgcctcttt aggggctaat attgcagcta aacgggtagc
    10801 aggagctcat gttgccttca acgttatcgg aacagtcgtc tgcgttattt ttctagttcc
    10861 ttttactgtc ctgattcatt ggtttgaagc tacgctaaat ctagcaccgg aaatgaccat
    10921 cgcctttgct cacggaacct ttaatattac caacaccatt gtccaatttc catttatcgg
    10981 agctctggct tactttgtaa ccaagattat tcctggagag gacgaggttg tcaaatacga
    11041 acccttatat cttgatgaac atttcatcaa acaggcccca tctatcgctc taggaaatgc
    11101 taagaaagag ctcttgcact taggaaacta cgctgctaaa gcctttgacc tttcctataa
    11161 gtacatcatt gacttggatg aaaaagttgc tgaaaaaggg cataaaaccg aagaagcaat
    11221 taacaccatc gatgagcaat taacacgtta tctcattgcc ctttcaagcg aagctctcag
    11281 ccaaaaagaa agtgaagtgc ttaccaatat ccttgattcc tcccgtgatt tggaacggat
    11341 tggagaccac acggaggctc tactcaatct gactgactat cttcaacgga aaaatgttga
    11401 attttctgat gccgccttga aagaattaga ggaagtttac cgccaaacta gtgactttat
    11461 caaagatgct ctggatagtg tggaaaacaa tgatattgaa aaagcacgca gtcttgtaga
    11521 acgtcatgaa gcaatcaata agatagaacg tgttctcaga aaaacccaca tcaaacgtct
    11581 caacaaaggc gaatgttcaa cacaagctgg ggtcaacttt atcgacatca tctcacacta
    11641 cactcgtgta tcagaccacg ctatgaacct tgctgaaaag gtttttgcag aacaaatcta
    11701 agaaccaaga agctatccat cataattgga tggcttttta ctttttccta agcaagacta
    11761 ggctgaatga aactgaaaga gtattctgga gatatatagt atattgaaac tagaatagta
    11821 cacctctatt tctaaaacat tcttagaaat cgatttgact gtcctgatag agatgttcac
    11881 atcttatttc aattcactat acaatccagt tcctaaaact ttgctagaaa ttgatttggc
    11941 tttccgaatt aatttgttca aattttattt catttcaata tactgataaa tccattcact
    12001 agcacagggc aagagggagt cgctatttat ttcaacggta gcaggtacat acagaggact
    12061 cctatctcag atatactgca atatcatcgt atgcaaaaaa cctctgagat ttttctcaaa
    12121 ggttctgatt ttgctaattg ctgttctcaa ccgctgcagt aacaaaggca gtgtagagtt
    12181 cttctggtcg gtttggacgg cttgacagtt cagggtgata ctgacaagct acaaagaatt
    12241 tattttcagg aatttccacg atttctacca aacgattgtc tggagaaact cctgaaaaga
    12301 caaaacctgc tgcctcaaac tgctcacgga aggcattatt aaactcataa cggtgacggt
    12361 gacggcgttg caccacttct tgattgtgat aagcagcagc agccttagag ccacgtttca
    12421 acttagacgg ataaagtccc aaacgaaggg ttccacccat atcctcaata tcaatctgat
    12481 cacgcatgat atcaatgata gggtattttg tttctggtgc aagctctgca gaattggcac
    12541 cttcaagacc taaaacgtga cgagcaaact cgatacatgt caactgcatt cccaagcaga
    12601 ctcccaacat tggaacatca ttttcacgcg catagcggat ggcttggatt ttcccttctg
    12661 taccacgttg accaaaacca cctggtacga tgatcccgtc cgcatcagac aagagttctg
    12721 ctacattctc tgctgtcaca tcattggcat tgacccaatt gattttaact tctacatcat
    12781 tgacatagcc agagtgtttc aaggcttcga ccactgagat ataggcatct tgcaactcca
    12841 catacttacc aacaagggaa atcttaactt gtttcttgag gttcatgacc ttgtccacca
    12901 tggctgacca ttctgtcata tccgctgctg gtgcgtctaa tttcaaatga tcacaaacaa
    12961 tttggtccat cccttgtgcc tgcaagttca gtggaatttg gtaaaggtgt tcaacatcca
    13021 acgattcgat aacggcttct ggtgccacat cacagaactg ggccagttta tttttaattc
    13081 cttgaccagc tggctcttct gtacgaataa ccaacatatt tggttggatt cccaagccac
    13141 gcaattcttt gacagagtgt tgggttggtt tggttttcat ttcaccagca gccttgaggt
    13201 aaggaagcaa ggttgtatgg atatacatga cattatccgc acccacatct gccttcatct
    13261 gacgaagagc ctctaggaat ggcaaggact cgatatctcc tactgttcca ccaacctctg
    13321 tgataatgac atcagagtcg gtcgttagag cggcacgctt gattttttct ttcaaagcat
    13381 ctgtgatatg aggaatgact tgaacagttg ccccaaggta ttctccacgg cgttctttac
    13441 gaagaacttc actgtaaatt ttcccagttg tcacgttgga atatttgttg agattgatat
    13501 cgatgaaacg ttcatagtga cccaagtcca aatctgtctc agctccgtca tctgtcacaa
    13561 aaacttcccc gtgctggtaa ggactcatgg ttcccggatc aatattgata taagggtcaa
    13621 acttttgaat ggttactttg agaccacgat ttttcaagag acggcctaga ctcgctgcca
    13681 caatcccttt cccaatggac gataccacac caccagttac aaaaatatat ttcgtagaca
    13741 tagattcctc tttctaaaat gctcaaggtc ttgttaacta cgaggggata tagaaaacaa
    13801 gaccctacta ataagaataa tctgggacga ctgcaccaga ttcacaacta atactcttcg
    13861 aaaatctctt caaaccgcgt caacgtcgcc ttgccgtaga tatgtgttcc tgactttgtc
    13921 agtcttatct acaacctcaa aacagtgttt tgagcagcct acggctagtt tcctagcttg
    13981 ctctttgatt ttcattgagt ataaaataca agaagataac ttcttgaaaa aacaaaaata
    14041 gctccctaag aactagggag ccccgacctc taaaagaggt gcccaaacaa tatgatacct
    14101 aaaaatagga taattgtcaa tagctaactc ttattcctcg ctggtttcag cgtcgtcatc
    14161 tgaaaactcg tcgtcgtctt cgttgagatc cacatcttct cccaagtcat ctggagcgat
    14221 ttcgttgatt tctgcatcat aagcttccac ttcatttttt tcatcatctg gattttcatc
    14281 atcgtatgaa agagctggat ctgcttcgta tgcatcttcg tcttctggat catctgcatt
    14341 gtagtcaatg gcatctgaat caccatccat aaaggcattg acacgttttt tcttagcttt
    14401 tggtgctact tcatcgtcgt cattttcttc aagagcgatg atttcttcgt cgatttcgtc
    14461 cacaccatac catgaacgaa gaccccattt gttgtcccca agtgagatga agctaccgtc
    14521 aaagttcaac tctgtgtaga acaaaggcaa agcttcgcgg atatcgctgt ttgatgttcc
    14581 aaggtagttt tgaatttcgt ttacaagatc gctaaaatgc atctcatgat cgcgaccacg
    14641 aagttccaag atagcacgcg ctacctcaat catagatagt tcactttttt cttgcccagc
    14701 aaatacttct aattccaaag cgtttctcct catttatact actatcgcca gagcgaacag
    14761 actctgacct cattttatca tttactcttt attttacgat aattttgcgg aatagtcaaa
    14821 ggttaagggg gagaaagtgg caggattaga ctaattccaa tataaaactc cttccttttt
    14881 ctgttgctcc attttccaca aatccaagcg acttgaaaca cctcctagaa gcataattgt
    14941 aggtgtagat ttccttgact ctcaattctt tccatccttt tactcgagcc aattcaatca
    15001 aagcacttag aatctttttt ccaagtcctc gatgttggta agcggaattc ccaatcacaa
    15061 tggggagatt atcctgagat agtgtaatat ccccaattgg aaaccattct cccttctcct
    15121 tgacttcaat ccaaaaaagc tcaccatgcc gatccaaata ggaatacatg gcttccaagg
    15181 tcgcttgact gtaaggaagc ttcaccccat ctacgaggta aaccaagttc acatcctgat
    15241 accaagcaaa agcttcctca caatgattga ccttataata aggaacaaaa cacaatgcac
    15301 taccttttca acaaaagagt tgctgcttga ttaaaaccat cacaccagtt ataccatttt
    15361 gcttcatacc catcttgagc taggatacga tcttctaaat caaaaacaga gtaaatcttt
    15421 ctttcctcgc aagcttgcgc atagagatga tatagttcat caccaccatc tctatcccac
    15481 tcagcagaaa tcgtatcccg acctgccaat aaagcctgat aagccctgtg atgcccatct
    15541 gtaatcagca aacaatctcc aaaggcaaga atactgattg gatcgacttg gattgtttct
    15601 gccgactggt aaagcatctg aatatcttgc aacttctttt ctgataaata tagttgagtc
    15661 ggatgaagat ctgctatatt gactttcatt tctttctcct caagggaatt cgatactcac
    15721 ttctgtttgc ctttaaatcg ccattggaag cggagcttgt catagaaggg aaactcgata
    15781 aacaggactc ccaagcccac acagagactg gcaaggacgt ctgatgggta atgaactccc
    15841 agatagactc ttgataccag cacactgact aggtagaggc caaggacgat ttgtacgatt
    15901 tttctccaga ctggagtttt aattcgttga ctgagaatga caatcagagt acctaccatc
    15961 aaagttacag ccagagaatg cccacttggg aaggaaaatc ccttctcttc caccagatgt
    16021 aagatagctg gtcgtgggcg ctggtagata tttttaaagg tcacgattaa aagacctgcc
    16081 aaagccagat ttcccagcat gaagaaactt tctatcttcc atcgcttacg ataaaagaca
    16141 aaagctgtaa tgacaaccca agtgataatc actgggatat caatcagacg tgtgagggat
    16201 cgaaaaagaa tagtcaaata atctggtaag tctcctcgaa tggcagtctg aatcgattgg
    16261 tcaaaattga ccaacatttc agggtaaaat ttgaccatat agccaagaat aacgaaaagt
    16321 aaaagggcaa aactgccctt cattaaaaat gtttgtttat ctttcatagt gttttaaggt
    16381 tggtttcaaa aggacgtaca acaaccagaa tgaaacggaa aagattacac cctcaatcaa
    16441 gttaaaaggt aataccatgg tcattaggta gttggaaagt cccaaaattt ttccaatatc
    16501 aaagttagca aacttagcgt acaaaggaac agcataaaca tagttgagaa ccaacatggc
    16561 caaggttaaa ccaacagttc cagctagaga gcctagtagg aaacgaaggg ttgtccgttc
    16621 ctttttccaa atcaaagcaa atacgatgac aaaaactccc aaagctacga tattcatcgg
    16681 caaaccaatg taagtattca ctccttggct gttaagaagc aatttcaaga gtgagcgaag
    16741 caagagcact cctagagcag caggcaaatc catgaccacc agacccacaa ggactggcaa
    16801 gatactaaat tcgatcttga ggaaagatgc cgctggtaaa agcggaaagt caaagtacat
    16861 cagcacaaat gagatggctg ataaaattgc aatggtcgaa agtcgacgtg tgtttgtcat
    16921 aacaggttcc tccaattttc tataaaatca gaagaagttg gaaaggattc ctctatctat
    16981 tctcactttt tatatcccaa aagttccctc ttactctatt aaagaaaaac aaagcaagtg
    17041 gttacaatcc ggctataaat ctatcaaaac agacaaagct attctttcgt cttctcccat
    17101 ccagactata ctgtcggttg tggaatctca ccacatcagc ttgcgctcgc ggacttcttt
    17161 aaagagaagg aaatagagat ttttctttta gactaggcga caagctgtag gcataacttc
    17221 tggttaggca aagcttgtca acaaagtata aaaggaaaag atcatttcct agttaaagtc
    17281 accgccggtc gggaatcaca ccctgccctg aagactcttt tatcataaca aaaaacgctt
    17341 gcaagcgcaa gcattttgtt cattttcatt ttttatttca atttatccac ttcataggta
    17401 tggacgagct caagacctgc aaagttctgc tggcgaagag cctcatagac aatcatgcag
    17461 acggtattag acacattgag actgcggaca tgttcatcat tcatgggaat acggagagcc
    17521 ttctcaggat gttctcgcat aaaatcttca ggtaagccct tgtcttcacg tccaaagaga
    17581 aaataatggt cctcgtcagt cgataaatcc acctcagaat agactttttc cgcgaatttc
    17641 gaaatcagat agagttttcc cttcatctga gatatgaaat cttccaaact ctcgtaaaaa
    17701 taaatctcta gcttatccca gtaatccaat ccagcccgct tcatcttgcg gtcatcaata
    17761 ggaaagccca taggcttgat gatgtggagg ggagaattgg tcgcagcgca agtacgcgcg
    17821 atattgcccg tattttgtgg aatttgaggt tcaaataata caatgtgatt tgtcatgact
    17881 tgcttccttt caccattgca aaaaaatagc cacactgccc ggagtcaagc tcagcaaaca
    17941 gcgtggttaa ggcatcgtta acttacctca caacaggttt gaagtaaatc agcgaaacta
    18001 ctttcttagt ataacacttt cagaatcatt gtcaatagaa atgacttgat tttttcaatt
    18061 ttttcaagct atttccaagg gttgtaaaat cgtccctgat tctgcaagat aagtagtaaa
    18121 ctaactacta aaaacaaggt tgccaagagc aaggtaatat agtctccttt tttcaaggcc
    18181 tgataactat accatgtgcg ttttttctct ttcccaaagc ggcgaagctc catggcagtc
    18241 gcaatggtat caatgcgttc tagcgagcta aaaatcaagg gcgtaataat gagcagattg
    18301 cctttgattc gttgcataag agaagctttc ttggataatt ccatcccacg cgcctcctga
    18361 gacatcttga tagtaaagaa ttcttcctgc aaatctggaa tatagcgcaa ggtcaggctg
    18421 acagaataag caatcttata gggcacacca atttgattta aactggaagc aaactgacta
    18481 ggatgggttg tcatcaaaaa gataatagcc agaggaatgg tgcaaagata cttaatggcc
    18541 aaatttagca gataaaagag ctcctggctg gttagagtgt agacaccgat tccctgccaa
    18601 atcacacttc tctctccata aagtccaacc ccatactcgg gagaaaagag atagaccatc
    18661 aaaacgttta aaacggcaaa tatcgtcgca aaaacggcta caaaggaaac atctttaaag
    18721 cgaatttctg ataaatagag gagaaagact gaaaagatgg caatcagcaa gagcattctg
    18781 gtatcatagc taatcatggc cgccaatgat accagaatga aaaagagaag tttcccagct
    18841 cctgacaagc gatgaatcac agtatctcta tgctggtaac cgattaattt agcttgcatc
    18901 cctctctcct ttctttgtaa aatgccgtta aatccagtgg atccacatct agtttcttag
    18961 ccaagttaaa gatggaggtt tcttttagat tggcttttac taacagctca ggatcgctca
    19021 acagactggc tggaacagta tcggcaatca attctccatc caccatgaca agggctcgat
    19081 ctgaataatc cagcatcaat tgcatatcat gggtaatcat gacaatggta tgcccttgtt
    19141 gatgcagttc ttcgagaaat tccataatct cagtatagtt cttctggtct tgacccgcag
    19201 tcggttcatc taggaggata atttcagcgc ctaagaccaa aattgaggca atagtcacac
    19261 gttttttctg accaaatgac agggcagaaa tgggccaatt acggaattca tagagaccac
    19321 agattttcaa ggtttcatag actctcgttt caatttcctg ctcgtccaca cctcgcaaac
    19381 ggagtcccag agccacctca tcaaaaatca tattggttga aatcatttga ttgggatttt
    19441 gcagcacata gcctactcgt tccgcccgct ctgcaacaga atctcctttt atatcctgtc
    19501 cttcccaaag atagcggcct tcagtctgga taaagctact caaggccttg gctagagttg
    19561 acttccctgc tccatttttt ccgacaatgg caatcttttc acccttttta atatctaaat
    19621 gaagagattt taaaatcggt ctatcatcat aagaaaacga cacgtcctct agtctaaaga
    19681 gtgactgcaa ttctggggtt tcttttacca gttccgtccg caactgaacc tgaccttttg
    19741 agatatacaa gttatccaga tctgctaatt gttcttcctt gactaaatcc acacccaatt
    19801 gacggagagt ggtgagataa aggggttcgc gaattccatt ttgggtcaac aaatcagtcg
    19861 ccagtaactg gtcagtgctc ccattaaaaa ggatacgacc atcgtttatc aagacaatcc
    19921 gatccacagg gcaatgcaga acatcctcca aacggtgctc gataataagg gtcgtcgtcc
    19981 cctcctcctt atgaatctgg tcaatcaatt cgataatatc ctgacctgac ttgggatcaa
    20041 gattggcgag tggctcatca aacaagagaa tcggactctc atcaatcaag acaccagcca
    20101 gactgactcg ctgcttttgc ccaccagaca aatcctgagg acgctgattc agtaaagaaa
    20161 taaggtccag cttttcagcc catttataaa cacgaccttt catctcatct agggctgtca
    20221 catcattttc cagagcaaac gccaaatctt ctgccacaga caagccaata aactgcccat
    20281 ctgtatcctg caaaactgtg ctaaccagat gagacttatc atagatgctc atatcaaagg
    20341 ctacttgacc ctttatcaaa aattctccat atgtctgacc cttgtaaata ttgggaataa
    20401 tcccattcaa acactgaccc aaggtagatt tacctgaccc agatggtcca acaattaaga
    20461 ctttctctcc cttgtaaatg gtcaagtcta tcccttgcaa ggtcggttct tgttgtgttt
    20521 cataccggaa agagaaatcc ttccactcaa ttatagcttc tttcatctta ctctcttcat
    20581 tcgcttctta gacttctatt ttatcataaa tcaagccctt cttgcagtct ctcctcttaa
    20641 aatcttagcg ccaaaaagat tcctatccta gcttacttgc ctaactaatc tataaacatc
    20701 gaaaaagact agttgcccag ccttccccat cattttatac tcttcgaaaa tctcttcaaa
    20761 ccacgtcagc ttcgccttgc cgtaggtatg gttactgact tcgtcagttt catctacaac
    20821 ctcaaaacca tgttttgagc tgacttcgtc agttctatcc acaatctcaa aacactgttt
    20881 tgagcaacct gcggctaact tcctagtttg ctctttgatt ttcattgagt attagtcctt
    20941 tttcaaactt cctgcacgag tttgagttcc tgcataggca agtaagagaa gagttcctgc
    21001 aatagctaca gatacaccat tggcaattcc cgcaacaatc ccttgtgcaa atactttttc
    21061 tgccgcttct tgataaatca caacatctcc aagtggtgcc aagacacccc aaacaagggc
    21121 atttgcaagt agttgaatga ggttaaaaat aagaatatct ttccagtcaa aaacaccatt
    21181 gatcactcga acgtactttc taaaaagtcc cacaactaaa ccaaagagtc cgctagcgat
    21241 aatccaagtc caccatagac cataaccagc aagagagtcc ttgattgcat gaccaatcaa
    21301 cccgacaagc aaaccgataa tcggtccaaa aataatagaa agtagcgctt gtaccgcata
    21361 ctgaagctgg atgcttgtat ttggaacagg ggttggaatg ttgatcatcc cgatgacgac
    21421 aaagagggca gcgccaattc cgacagcaac aacttgttta attgtaaatt tgatttccat
    21481 actattctcc tattttatcc ttctattttc tttatttcaa tggtccaaga tgaaccgaca
    21541 cctacattat aggccttggc aaaggaacct tggttgatag ccaaacctaa acgatagaga
    21601 gagttgatgt aaaggatggg ttgcccaatt ctcacatctg caaatgattt gccatagaca
    21661 acctgatttt gatagaccag catatcagca tgatagatgg tcacttcaaa acgatcacca
    21721 aattctggtt ccagcttgta aaattcttcc cgtgtgatag aggtccaaag cgaaccgaaa
    21781 cgcacatcca gaatatcaat ggctcccttc accagatgat cttctatgat ggtcgctacg
    21841 actggaagct ctacaatctg gtccacactg agctctggcc ctacttcctc aaaagtaatg
    21901 tgaccactgg ccagtttagc accagtatag gcatagacat cacgaccgtg gaaggtataa
    21961 gaatgctctg tgttttgacg cctattggcc acctcagaaa tctcacgaat ggctacaatg
    22021 ccaacgtgtt tcttgataaa ggaaagcgtc ccattatctg gcgtgacaat gtattgattt
    22081 tttgcagtct tggcaactac actcttacgt ttcgaaccga cacctggatc gacaaccgat
    22141 acaaacgtcg ttccctcagg ccagtaatcc accgtctgaa agagacgata gctcccctca
    22201 aaaatattat aaggcgtgat atcgtgcgtc aagtgatgga tttttaaggt tggagactct
    22261 tctaaagcca ctccaatcat agccgatacc gcaccatcaa ccagaccaaa gtctgattgt
    22321 aataccagta aattattatt cattttatct ccttgtatat cctttatcat accatatttc
    22381 agagaaagtc gctaatagct atttcaattg gttagggtat agttttacct tatagcgaat
    22441 tttcactaaa agctcttgtt attagctagt aggtgcattt tttcttgaaa aagggtatga
    22501 tagttacatc atgaaaaagt aggttttatt atgaaaatta tccttgtcgg agggggaaaa
    22561 gttggttttg ccctctgtcg ctccttggtt gcagaaaagc atgatgtttt gctgattgag
    22621 caagacgaag ctgttctcaa tcatattgtc agtcgctttg atatcattgg tatccttggt
    22681 aacggggccg attttgccat tcttgagcaa gccagcgtcc aagattgtga tatctttatc
    22741 gccctgactg agcacgatga agtcaacatg attgcagcag ttctagccaa gaaaatggga
    22801 gctaaagaaa ctatcgttcg ggtgaggaac cctgaatatt ctaactctta tttcaaggaa
    22861 aagaatattc tcggtttttc tcttatcgtt aatcctgagc tcttggctgc ccgcgctatc
    22921 gcgaatatca ttgacttccc caacgccctg tctgtcgaac gctttgctgg tggacgcgtt
    22981 agcctcatgg aatttgtcgt caagtccacc agcggtcttt gccaaatgcc catttctgat
    23041 tttcgtaaaa aatttggtaa tgtcattgtc tgtgcgatag agagggatca tcaaattatc
    23101 attccaagcg gtgacatgac tgtacaggat aaagatagaa tctttgtcac tggtaaccgt
    23161 gtcgatatga tactcttcca taattatttt aaatcacgcg ccgtgaagag ccttctcatc
    23221 gttggagcag gtagaattgc ctattatcta cttggtattc tcaaagatag tcgtatcgat
    23281 acaaaagtca ttgaaatcaa tcctgaaatc gccagcttct ttagcgagaa attcccaaat
    23341 ctctacatcg ttcaaggaga tggaaccgca aaagatatcc tgctggaaga aagtgctcaa
    23401 cactatgatg ccgttgcgac tctaacagga gtcgatgagg aaaatctgat tacatctatg
    23461 ttccttgaca gggtaggtgt acagaaaaat attactaagg tcaatcgtac cagtctcctc
    23521 gagattatca atgcgcctga tttttcaagt atcatcacac ctaaaagcat cgctgtagat
    23581 acgattatgc actttattcg tggtcgagtt aatgcccagt attcagacct tcaagccatg
    23641 caccatctag ccaatggcca aatcgaaacc ctgcaattcc atatcaagga agccaataaa
    23701 atgactgcca aacctctttc tcaactgaaa ttgaaaaaag gggttcttat tgcagccatt
    23761 attcgaaagg gcaagactat tttcccaact ggggaggata tgttggaagt tggagacaag
    23821 ctcctagtaa caaccttgtt gccaaacatc accaagattt atgacttgat tgcgaggtaa
    23881 gaaatgaata aaagtatgat tcgttacctc ctttcaaagt tacttttgat tgaagctgtt
    23941 ctcctcttgg ttcctgtggc tatcgctgtc tattaccgtg aatcgagcca agtctttaca
    24001 gccctctttt cgaccatagg gattctcgta ttgttaggcg gttcagggag tttacagaag
    24061 ccaaaaaatc aacggattta tgccaaggag ggagtcttga tcgttgccct ctgttggatc
    24121 ctttggtctt tctttggtgg tctccccttt gtcttttctg ggcaaatccc tagccttatt
    24181 gatgcctttt ttgaaattag ttctgggttt acaactactg gagcaagtat tttgaacgac
    24241 atttcggttc tcagccgttc cctcctcttc tggcgaagtt ttacccactt gattggaggg
    24301 atgggagtgc ttgtttttgc acttgctatt atggacaatg ccaaaaatag tcacctagaa
    24361 gtaatgaagg ctgaggttcc tggtcctgtc tttggtaagg ttgtatccaa attaaaaaac
    24421 actgcccaga tcctctatct cctttacata gccctctttt ccctctttgt catcatctat
    24481 tatctagccg gtatgcctct ctacgatagt tttgtcattg ctatggggac agcgggaact
    24541 ggaggattta ccgtctataa cgatggaatt gcccactatg gtagctcact gattacctat
    24601 ctggttagta taggagtttt ggtttttggg gtaaatttca atctctacta ctaccttatg
    24661 ctccgtcggg ttaaggcctt ctttggagat gaagagcttc gggcttactt ggtcatcgtg
    24721 ctggtttcta caggcttgat tagcctcaat actctctacc tctacccagg gttttcaaag
    24781 agctttgaaa tgaccttctt ccaggtttcc aacatcatta caacaactgg ttttggatat
    24841 ggagatatta ccaactggcc cctcttctcc cagtttatcc tccttttcct catggcaatc
    24901 ggtggctctg ctggatcaac tgcaggtgga ctcaagatta ttcgaggcct catcctttca
    24961 aaaattgcca aaaaccaaat tttgtcaatc ctatcgcccc accgtgtttt gaccctccat
    25021 gttaataaaa tggtgattga caaagatacc cagcataaga ttctcaagta ctttgtcatc
    25081 tatgctatga ttttgctagc gcttatcttt attgtcagcc tagatagcaa tgattttctg
    25141 gtcgtgacca gcgctgtctt tagctgtttc aataatatcg ggcctattct aggaaccact
    25201 tctagtttct caatctttag tcccatctca aaaattctcc tctcctttgc aatgattgca
    25261 gggcgcttgg agatttaccc aatcctcctt ctctttatga aaagaacttg gtctaagaga
    25321 taaaatacaa aatccctctt tttacaattg tgaaagaggg atttttatat taaaaagggg
    25381 gaacgttttt aaatccaagc ctgtctgtaa gcaaagttct tactcaaata aaagatggaa
    25441 aaacttacta cacagcttgg taatgctcca atacaaattg taaggcttta cttggattct
    25501 ttgttaaatg aatatattcc attcctctgg tagcaactat ctgaatacct aatctttctg
    25561 catccacctt catctctcta taataactcc gaacttgtgg agccagaata attaaatcat
    25621 aaacacccat aatatcataa tgagctccgt acgctcctga attcgcaatc actctaactt
    25681 ctgctaattg agcgccctcg ttaattgcat tggctaatta cgcacttgtt ccagaccctg
    25741 cacaaagaac cagtactttc aactccttac tttttattac atctatctca ccaggcatta
    25801 tttcacttgt atcctcctct aaaataatat catttgagct tgcaatatct ataacctgta
    25861 gtaaatatct cgtatttaga caatatgaaa acaagacgac ttccatatag gaaaccgcct
    25921 tctcgctatg ttgagtgatt tatattaaaa taacttttct tctagctgca ttttattatt
    25981 ataaaaacat tcatcataac ccccagaact taaataacaa tttttat
//
LOCUS       DAVKGC010000015        25795 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKGC010000015 DAVKGC010000000
VERSION     DAVKGC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.015"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..540
                     /locus_tag="VW049_000521"
     CDS             <1..540
                     /locus_tag="VW049_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9431692.1"
                     /translation="LSRAMSRVRVQIMNQLDRKSHEYKAIKRYWKLIQQDSRKLSDKR
                     FYRPTFRMHLTNKEILDKLLSYSQDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQ
                     VHPIFQTVFKTFLKDKEKIINALQLHYSNAKPEATNNLIKLIKRNAFGFRNFENFKKR
                     IFIALNIKKERTKFVLSRA"
     gene            complement(661..2181)
                     /locus_tag="VW049_000522"
     CDS             complement(661..2181)
                     /locus_tag="VW049_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9431693.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENVIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(2174..2902)
                     /locus_tag="VW049_000523"
     CDS             complement(2174..2902)
                     /locus_tag="VW049_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431694.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(2917..3681)
                     /locus_tag="VW049_000524"
     CDS             complement(2917..3681)
                     /locus_tag="VW049_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9431695.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(3685..4128)
                     /locus_tag="VW049_000525"
     CDS             complement(3685..4128)
                     /locus_tag="VW049_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9431696.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(4810..5106)
                     /locus_tag="VW049_000526"
     CDS             complement(4810..5106)
                     /locus_tag="VW049_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9431697.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(5128..6192)
                     /gene="pepA"
                     /locus_tag="VW049_000527"
     CDS             complement(5128..6192)
                     /gene="pepA"
                     /locus_tag="VW049_000527"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9431698.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            6593..7294
                     /locus_tag="VW049_000528"
     CDS             6593..7294
                     /locus_tag="VW049_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEU9431699.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SHASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTTNVAGMNQV
                     LKVLSEVGIQKIMFSSTASLCGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            7717..8676
                     /locus_tag="VW049_000529"
     CDS             7717..8676
                     /locus_tag="VW049_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9431700.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            8666..9622
                     /locus_tag="VW049_000530"
     CDS             8666..9622
                     /locus_tag="VW049_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9431701.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            9619..10371
                     /locus_tag="VW049_000531"
     CDS             9619..10371
                     /locus_tag="VW049_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9431702.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            10467..11432
                     /locus_tag="VW049_000532"
     CDS             10467..11432
                     /locus_tag="VW049_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9431703.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(11656..11898)
                     /gene="yidD"
                     /locus_tag="VW049_000533"
     CDS             complement(11656..11898)
                     /gene="yidD"
                     /locus_tag="VW049_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9431704.1"
                     /translation="MKRILIALVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(11898..12620)
                     /locus_tag="VW049_000534"
     CDS             complement(11898..12620)
                     /locus_tag="VW049_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9431705.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(12607..13176)
                     /locus_tag="VW049_000535"
     CDS             complement(12607..13176)
                     /locus_tag="VW049_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9431706.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(13195..13923)
                     /locus_tag="VW049_000536"
     CDS             complement(13195..13923)
                     /locus_tag="VW049_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9431707.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(13923..14663)
                     /gene="xerD"
                     /locus_tag="VW049_000537"
     CDS             complement(13923..14663)
                     /gene="xerD"
                     /locus_tag="VW049_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9431708.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(14654..15115)
                     /locus_tag="VW049_000538"
     CDS             complement(14654..15115)
                     /locus_tag="VW049_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9431709.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(15112..15633)
                     /locus_tag="VW049_000539"
     CDS             complement(15112..15633)
                     /locus_tag="VW049_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9431710.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(15609..16580)
                     /locus_tag="VW049_000540"
     CDS             complement(15609..16580)
                     /locus_tag="VW049_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9431711.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(16577..17371)
                     /gene="racE"
                     /locus_tag="VW049_000541"
     CDS             complement(16577..17371)
                     /gene="racE"
                     /locus_tag="VW049_000541"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9431712.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(17578..17826)
                     /locus_tag="VW049_000542"
     CDS             complement(17578..17826)
                     /locus_tag="VW049_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9431713.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(18082..19707)
                     /gene="treC"
                     /locus_tag="VW049_000543"
     CDS             complement(18082..19707)
                     /gene="treC"
                     /locus_tag="VW049_000543"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9431714.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(19885..21852)
                     /gene="treP"
                     /locus_tag="VW049_000544"
     CDS             complement(19885..21852)
                     /gene="treP"
                     /locus_tag="VW049_000544"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9431715.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            22037..22747
                     /gene="treR"
                     /locus_tag="VW049_000545"
     CDS             22037..22747
                     /gene="treR"
                     /locus_tag="VW049_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9431716.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(22779..23582)
                     /locus_tag="VW049_000546"
     CDS             complement(22779..23582)
                     /locus_tag="VW049_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9431717.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSMDEGDLLLAGKSIKNLSVRKRAKDIARVFQAPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQLTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLR"
     gene            complement(23595..24484)
                     /locus_tag="VW049_000547"
                     /pseudo
     CDS             complement(23595..24484)
                     /locus_tag="VW049_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(24496..25434)
                     /locus_tag="VW049_000548"
                     /pseudo
     CDS             complement(24496..25434)
                     /locus_tag="VW049_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7448 a         6041 c         4904 g         7402 t
ORIGIN      
        1 cttagccgtg ctatgagtcg tgtgcgtgtt caaattatga accaattgga tcgaaaatcc
       61 catgaataca aggctatcaa gcgctactgg aagctcatac aacaggatag ccgtaaactg
      121 agtgataagc gattttatcg tcctactttt cgtatgcatt taaccaataa agagatttta
      181 gacaagcttt tgagctattc acaagacttg aaacatcact atcagctcta tcaactcttg
      241 ctgtttcact ttcagaataa ggaaccggag aaatttttcg gacttattga ggacaatctt
      301 aagcaagttc atcctatttt tcagactgtc tttaaaacct tcctcaaaga taaggaaaag
      361 attatcaacg cccttcaact acactattct aacgccaaac cggaagcgac caataatctc
      421 atcaaactta tcaagcgcaa tgcctttggt tttcgaaact ttgaaaactt caaaaaacgg
      481 atttttatcg ctttgaacat caaaaaagaa aggacgaaat ttgtcctttc tcgagcttag
      541 ctgacttcaa cccactacag ttgacaaaga gccaaaaaag cccagacgaa attgtctgag
      601 cattctttta tctagtcgtt taaggaagtt gagttcagta tgtttaaagt ctctgtccca
      661 tcatttcttc aacaaacctt gttcttggag aaactccttg gctacttgct ttgctgactt
      721 gccttcaaca ccgacttggt agttgagctg gctcatctgg ctttctgtaa tcttaccagc
      781 caatgtatta agaactcttt ccaactctgg gtgtttcttg agaagagctt ctttcatgag
      841 tggagcccct tgataaggtg ggaagagttg cttgtcatct tccaagacct gtaaatcata
      901 acgctccaat tccgcatcag tcgaataggc atccgtgatt tgaatatccc ctgactgaat
      961 tgcctgataa cgaagagctg gctgcatagt cgccacattg agattgagac catacattga
     1021 ttgcaagccc ttatttccat cttcacggtc gttaaactca agcgtaaagc ctgccttcag
     1081 ctgcccttcc acttttttca aatccgaaat ggtcttcaag ccatattctt gagcaatctt
     1141 tttcggaaca gctacagcat aggtattttg ataagacata ggtttgagat aggctagatg
     1201 atcctgcttg gcaatgccat cacgcgccac ttgataaacc tgctctggct catgactcac
     1261 cttgggtgat ggttgaagca aactttcagt caccgtaccg gtaaattcag gataaatgtc
     1321 aatatcacct tttttcagag cttcataaag gaaacttgtc gtcccaaaat tcggtttaac
     1381 agtcgcagtc atgctggtat tttcttcaat cagcaattta tacatattag ccaaaatttc
     1441 tggttctggg cctattttcc cagcaataat cacgttttcc ttctcttttt gagccaaaag
     1501 ggctggacta taagacagac ccagtaataa agccaccaag gcaaaaccag agaaaatcgt
     1561 ccgcaatttc gctttttcca ttacttttag taggaagtta aaggcaatag ctagcactgc
     1621 agaagaaagg gccccaatca aaatcaaact ggcattatta cggtcaattc ccaacaggat
     1681 aaaggaaccc agtccccctg ccccaatcaa ggtcgccaag gtagccgtac cgataatcaa
     1741 gaccgctgcc gtccgaattc cagacatgat aacaggcatg gcaagtggaa tttcaaactt
     1801 cttgagacgc tcccatctgg tcatcccaaa ggcaatccca gcctcttgaa gactcggatc
     1861 aattcccttc agcccagtaa tagtattttg taaaatcgga aaaatcgcat aaatcactag
     1921 agctgtcaat gccggtaaag tcccaattcc catcaaggga ataaagagtc ccaacaaggc
     1981 cagagacggg atggtctgga aaatacctgc aatctgcaag acccagtcgg ccagcttctc
     2041 atgatagcga agaaaaacag ccaagggaat cgcaagcaaa atagctagta acaaggtcaa
     2101 aagtgacaac tgcaaatgtt gagatagagc tgtcaaccaa tcactaaaac gatcctgaaa
     2161 agttgcaatt aaattagtca tgaacactac ctccaaacaa gtctgctaca aagtctgttg
     2221 caggcgcttt taaaattgtc tcgggattcg ctacctggcg aatttctcca tcctgcaaga
     2281 cagcaatacg gtccgccaac ttcaaggctt catccgtatc atgggttaca aaaatcgttg
     2341 tcatcccaaa ctctttatgc aattcttttg tcagaacctg caactgtttt ctcgaaatag
     2401 catccaaggc cgaaaagggt tcatccatga ggaaaatctt gggctgacca atcatagctc
     2461 ggacaatacc gacccgttgc tgttctccac cagataattc actaggtaag cgatgcccat
     2521 actcggctac tggtaaacca accttagcca aaagctcttc tgttttcttc gtaatttctt
     2581 ccttgctcca ccccttcatt tcaggaatga gagcaatatt ttccgcaact gttagatttg
     2641 gaaaaagagc aatagcctgt aaaacataac cagtagaaag acgaagttca cgctcatcat
     2701 agtctttgat gcgcttccca tccatataaa tatttccatc agttggttcc aaaagacggt
     2761 taatcatctt gagcatggtc gtcttacctg acccagaagg ccctactaaa accataaatt
     2821 ccccatcctc aatctgtaag ttgacatctc tcaagacatc cttttctgtg tagcgcagtg
     2881 ctacattttt gtattcaatc attctttgtc ctcaatttaa aacttccctc gattggtcaa
     2941 gtcttctacc ttaggcatga cttccttatt atcccaatgc tccacaattt tcccgttctc
     3001 taaacggaag atatcgtact gggcataagc aacgccatca atctgagtct gaccatagct
     3061 aaccacatag tttccttgtc ctaagagttg gaaaacaaag tcaaaagtga cactatattc
     3121 agccacatag tttttataag cagcacttcc ttgtccaata tcatgattat gctgaatcaa
     3181 atcgtctgcc acataatcac tccactgctc tagctcccca ttttggaaaa tttctgtcaa
     3241 gaaacggcga accagctttt tattttctgc tttcttgtcc aaatccttga tttcaaaatc
     3301 tccaaaaatt tgatctagtt ggtcattttc aggtgttcga tagtagtcaa tgacatccca
     3361 atgctcaaca atacgaccat tctcatccgc acggaaagta tccgtcgtca cccattgagc
     3421 ttctccacca ttcagatatt gatgaacatg aacaaagacc agattgccat cctcaatggt
     3481 gcggacaatc ttaatctgac gctctggatg acgctcaaag aaatctgcaa agaaggctgc
     3541 aaatccttct ttcccgtcag gaacacctgt cgaatgttgg atataggtat cccctacaga
     3601 ctgggcttga gcctcagcaa ctcgtccgtc ttgaatggca tggatgtata ggttgtgagc
     3661 atttttcact tgttgtgaca tattctaaac ctcatttccc ttctctttca gattcgccaa
     3721 aattctttct tgaaaacctt caaattggtg aatttcttcc tctgaaaatc ctttgtaaaa
     3781 gatagtatcc aatttctgac tgacacgatg ccccacttct ttctgggact tgcctaactc
     3841 cgttaaaact aaatacttct tacgcttgtc ttttccacac ggactaacaa ttacaagctt
     3901 ttgttcctct agctttttta tcatagtcgt cagcgtatta ttcgcaagtc cagtcgcaag
     3961 cgcgatatct gtcgcagttg cgcagccagt ttcactattc cataaaaccg ctaaaatctt
     4021 gccctgttca cccctataaa gagcctcagg atcttgactc agtaactttt gaaaaatccg
     4081 cccattcaac aaacgaatat gatgggctag caaatgacca tctttcataa cacctccaat
     4141 ttatttcgat atcgaaatga ataaaacaat tgtaacactc atcgttctaa ctgtcaacta
     4201 tttcgattta gaaataattt ttgataatta tccacaccac catactccgg ctcaactaac
     4261 ttttaacgag agtttctaaa ctccttcgtc ctccagtcta caaaagcctt ccattcgtac
     4321 tatcctatat tttatgaggg gacacatttt tcctatcaga ccatttattt taaagataga
     4381 agtaaatcat aattgcttcc atctgttctt ttatagtata ttgaagttag actagagcac
     4441 tgtatcttct aaaacattga tagaaagcga tttgaatttc ccaatcaatt tgttcgtatt
     4501 tatagcattt cgaaactgga ataggacacc atgactgcta aaagatttct ataaattcat
     4561 ttaatttcct caatcaattt gttcatatct tatttcattc cgctataatt tcaccttacc
     4621 ctatcttttt cgtagcaccc ttcaaacagc ctatccccta ccgtttgacg attcctcact
     4681 tcgctccact tccattacag aagtttcttc actactatgg gctcggctga cttctcatga
     4741 ttccttgtta ctactatttg aacgctcacg agatagatct tacaaaaaat gctttgatcc
     4801 acaatggaat caaagcattt taaagagttc ctcatacata agcgcagaag tcgcagttcc
     4861 tctgtacttg gcttcttctc ttttgataaa gcgagccaag ttgagcaact caggtgctgg
     4921 atgtttggga tttaggagca attcacgatt gaccaggcct gagagacgaa ctgcctgcaa
     4981 ttgctcattt gtagtaggta gttttttagt agtctctagg agagcagcaa ctaaatcttc
     5041 actcaaatca tgtcgagcat gattgtaaag atcttttata aggctttcta ggtttggttc
     5101 tgccatccct accacctccc ttatggttta ataatgttta atcaaatcaa ccgttgaacg
     5161 atccaatttc ttcaccaagg cttgtaagaa agcttgcgct tctaggaagt catccattgc
     5221 atagagggtt tggtgagaat ggatataacg agcgcagaca ccgatagttg ttgatgggac
     5281 accaccattt ttcagatgag ctgcacctgc atctgttccg cctttaccac agtagtattg
     5341 gtacttgata ccagcttctt cagccgttgt caaaaggaaa tccttcatcc ctgggagaag
     5401 caagtgacct ggatcataga aacgaatcaa ggttccatct ccaatcttgc cttgaccacc
     5461 gtagacatca cctgctggtg agcaatcaac tgcgaggaag acttctgggt caaacttggt
     5521 tgtagaggta tgagcgccac gcagaccaac ttcttcttgg acgttagaac ccagatagag
     5581 ttcattgccg agtttttgac ctgataaagc ttcagctagc tcgcttacca tgaggacacc
     5641 gtagcggtta tcccaagctt ttgagatgat atttttttca ttggctgtca aaattgcaga
     5701 actatctggt acgatggtat caccaggacg gatgccaaaa ctttctgcct cagccttgtc
     5761 cgcaaaacca ccatcaaaaa cgatatcggc aatggctggc atggttggtc ccccctttcc
     5821 acgagtcaaa tgcggaggaa cagaacctga aatcacagga atttcatgac catcacgagt
     5881 caagagtttg aaacgttggc tgctaaccac catggggttc cagccaccga tttctacgac
     5941 acggaaggta ccatctggct tgatttcgct gaccataaaa ccaacttcgt ccatatgaga
     6001 agcgaccaag acgcgcggtg catccacagc ttctgaatgt ttgataccaa aaataccacc
     6061 caagccatct gtcaccactt catccacatg cggtgtcaac ttttcacgaa gataagcacg
     6121 gacaggcgct tcatgacctg agactgcagc aagttctgtt acttctttaa tttttgaaaa
     6181 taatgttgtc atttcagttc cttctttctt tcatccattt taccactttt tataggagaa
     6241 ggatagtggg aaggtggatt tctaagttag tatcttagtc ctgctctatc ttagaaaagg
     6301 atagtattct cttgcatgta gtgcaaaatc tagtaaacat tccaaaatta actcgaatat
     6361 ttatttccaa acaaaaaaac aatacaccat caaagttgtt tggatttttc atgaaattta
     6421 cagaaaatag ttgacttccc tttcttcttt ctttaaatat atagttggtt gagtttggaa
     6481 tagtacgctg tagctgctga aacatttcta gaaattaatt tgactttcct aatagagttg
     6541 ttcatatctt atttcaattt actatagtac aaaactagaa aaggaaaaaa tcatgaccaa
     6601 aacaattctt gttacaggtg ggactagcta cattggctcc cataccgtta aagctctttt
     6661 aaatgctggc tatcaggtgc atgtcctcga taatctctcc acaggaaatc gagcggctgt
     6721 ggatagtcat gctagcttta aagaactgga tgtttatgat gctagcgcct taaaggctta
     6781 cttagaagaa aatcagattg atgctgtcct ccattgtgca ggtgaaattg ttgtgagcga
     6841 aagtattgaa aatccaagta aatacttcac taccaacgtt gctggtatga accaagttct
     6901 caaagtctta tctgaagttg gcattcaaaa aatcatgttc tcttcgactg cttctctctg
     6961 tggaaataac tgtattgaca agccggtgac tgaagatacc ctgctcgacc ctgtcaaccc
     7021 ttatgcagag acaaaactga tgggcgaacg aatgatttac tggatggcca atcgctacga
     7081 ctggaaatat gttattttcc gttactttaa tgttgctggg gctgaaatgg atgcttcaaa
     7141 cggtctgcgt gtgaaaaatc caactcacat cattccaaat atcaacaaga ccgcattggg
     7201 acaacatgat agcttaaaaa tatttggaga tgactacgat acacgtgacg gttcatgtat
     7261 tcgagattac atttatgtct tggaccttgc ataggctcat gttaaaggaa tgaactacct
     7321 atttcaagaa gacagttctt ctcaaatctt taacttagga actaaaaaag gctatactat
     7381 caaagaaatc tttaaaactg ctgaagaatt actaaattaa aaaatcccac acgagattgt
     7441 tgctcgacgt gctagtgacc cagccagtgt cctagcagat gcttcaaaag ctaaacaata
     7501 tcttgattgg acggctagct actccctcga agatattatt ttatcagatt aaggtaaaaa
     7561 cattttagaa taggataaca aaggagaaag tcttgttgct ctctccttgt tgtatttatt
     7621 aaattgtcag aaattttatt gacagattag aaaaataatg ttacatttat atccgcagat
     7681 atctttcgat accaaatcta catgaaagga cggggtatga aactttctca ttatttaatt
     7741 ggcttacttc tactcctagt ctttctctct attagcattg ggaccagtga tttttcatgg
     7801 ggaaagctat ttgatttcga ccagcagacc tggctcctct ttcaagagtc ccgtctccca
     7861 agaactatca gtattctcct gactgcctct agtatgagta tggcaggcct tctcatgcag
     7921 actattaccc aaaatcagtt tgctgcaccg agtacagttg gaacgactga agccgccaaa
     7981 ctgggaatgg tgctgagcct ttttgtcttt ccatcggcta gtctgaccca aaagatgctc
     8041 ttcgcttttg tttcatccat cgtattcacc ctcttcttcc tagcctttat gaccattttt
     8101 actgtaaagg aaaggtggat gttgcctctg attgggatca tctatagcgg gattatcggc
     8161 tcagtcacag aagttatcgc ctatcgtttc aatctggttc agagtatgac tgcctggacc
     8221 cagggctcct tctccatgat tcagacccat cagtatgagt ggctcttctt aggcctcatc
     8281 atcctgataa ccgtttggaa attatcccaa accttcacca tcatgaatct agggaaagaa
     8341 accagcgaga gtttggggat ttcctactcc ctacttgaaa aactggccct ctttctggtg
     8401 gcgctaacga caagcgtcac catgattacc gtgggtggcc taccatttct cggagttatc
     8461 gttcccaatc ttgttcgcaa gcgctatgga gataatctaa gtcaaaccaa actcatggtc
     8521 gcactggttg gtgccaatct agttctggct tgcgatatcc tatcccgagt tctgattagg
     8581 ccctatgagt tgtctgtcag tctcttgcta ggaatcatcg gtagtctcgt ctttatccta
     8641 cttctctgga gagggggacg aaaagatgca gactaaaagc aaacatacca agctcttctg
     8701 gattctcatt attcttgcca tcggagcttg tcttctctac ttttggccca tcactcactt
     8761 gtcagccttt gcttggaagt tgcgttccca aaagatcatc gtttatctct tggtagccat
     8821 cgcgactggg atttcgacca ttagttttca aaccctgacg gaaaatcgct tcctgacgcc
     8881 tagtattttg ggaattgaat ccttctacgt cctactacaa accctactac tggtttttga
     8941 aagcaagttt cttcaacttg gcaaatcccc tatcttagaa ttcctagtct tacttcttgt
     9001 ccagtccctc ttctttctcg ccttacaagg ttacttgaag acactgatga agcaagacct
     9061 ggtcttcatc ctgctgatct gtctagcgct cagaagtctc tttcgaaata tcagcacctt
     9121 ccttcaagtc ctaatggatc caaacgaata cgataaactg caaaatagtc tttttgcctc
     9181 ctttcaacat ctcaacactt ccatcctagc catcggttct ctgatcatcc tcgctttgac
     9241 aatctttttc tttcgaaaag cagtcgttct agatgtcttg cacctgcaaa gagaaacggc
     9301 tcagatattg ggactcgatg ttgaaaaaga acagaaagaa ctcctctggg gaatcgtgct
     9361 tttgacctca acggccactg ccttggtagg acctatggcc ttcttcggct ttatgctggc
     9421 caacctcacc tacctgattg tcaaagacta tcagcacaag ttactcttta tagtggccat
     9481 tctggttgga tttattagct taaccttggg gcaagccttg attgaacgag tctttgcact
     9541 ggaaattcgt atcagtatga tcattgagag tgtgggtggc ttcttattct ttatcttact
     9601 atataggagg tctcgtcagt gaaactggaa aacattgaca aatccattca aaaacaggat
     9661 attttgcaag gcatttcgct tgaagtcagt cctcaaaaac tgacagcctt tattggtcca
     9721 aatggtgctg gaaaatcgac tctcctctcc atcatgagca gactaaccaa gaaagatcag
     9781 ggagttctca gtatcaaagg acgtgaaatc gagagctgga attcgcaaga actggcccaa
     9841 gaactaacca tcctaaaaca gaaaatcaat taccaagcca aattgactgt tgaagaactg
     9901 gtcagttttg gacgttttcc ctacagccga ggtcgactta gatcagaaga ctgggaaaaa
     9961 atccgagaaa ctctgaacta tttggaactg accaacttaa aagaccgcta catcaatagc
    10021 ctgtcagggg ggcaactcca gcgcgtcttt atcgctatgg tactggccca ggatacggac
    10081 tttatcttgc tggacgaacc actcaacaat ctcgatatca agcaaagcgt cagcatgatg
    10141 cagattcttc gacgactggt ggaggaactc ggcaagacca ttatcatcgt cctccacgat
    10201 atcaacatgg ccagtcagta tgcagatgaa attgtcgcct ttaaggacgg ccaggtcttt
    10261 agcaagggaa gaaccgatca aatcatgcag gctgacctgc tcagtcaact ttatgagatt
    10321 cccatcacgc tagctgatat caatgacaaa aagatctgta tctatagcta gtaacataaa
    10381 agctcaagtt agagaacctt cagtctctta gtcaataaga ttaagagact ccctaaatcg
    10441 ttatcacatt ttaaaaagga gaaattatga aaacatccct taaactttat ttcactgccc
    10501 tagtggccag cttcttgctc ctacttggtg catgtagtac aaactcaagc actagtcaga
    10561 cagagaccag tagctctgct ccaacagagg taaccattaa aagttcactg gacgaggtca
    10621 aactttccaa agttcctgaa aagattgtga cctttgacct cggcgctgcg gatactattc
    10681 gcgctttagg atttgaaaaa aatatcgtcg gaatgcctac aaaaactgtt ccgacttatc
    10741 taaaagacct agtgggaact gtcaaaaatg ttggttctat gaaagaacct gatttagaag
    10801 ctatcgccgc ccttgagcct gatttgatta tcgcttcacc acgtacacaa aaattcgtag
    10861 acaaattcaa agaaatcgcc ccaaccgttc tcttccaagc aagcaaggac gactactgga
    10921 cttctaccaa ggctaatatc gaatccttag caagtgcctt cggcgaaact ggtacacaga
    10981 aagccaagga agaattggcc aagctagaca agagcatcca agaagtcgct actaaaaatg
    11041 aaagctctga caaaaaagcc cttgcgatcc tccttaatga aggaaaaatg gcagcctttg
    11101 gtgccaaatc tcgtttctct ttcttgtacc aaaccttgaa attcaaacca actgatacaa
    11161 aatttgaaga ctcacgccac ggacaagaag tcagctttga aagtgtcaaa gaaatcaacc
    11221 ctgacatcct ctttgtcatc aaccgtaccc ttgccatcgg tggggacaac tctagcaacg
    11281 acggtgtcct agaaaatgcc cttatcgctg aaacacctgc tgctaaaaat ggtaagatta
    11341 tccaactaac accagacctc tggtatctaa gcggaggcgg acttgaatca acaaaactca
    11401 tgattgaaga catacaaaaa gctttgaaat aagccacttc aaactcaatt taatactcaa
    11461 tgaaaatcaa agagcaaact aggaaactag ccgcaggttg ctcaaaacac tgttttgagg
    11521 ttgcagatga aagctgacgc ggtttgaaga gattttcgaa gagtataaaa ttttgacatt
    11581 ctaaaacgca tcctatcaag ttcactcaaa cctgatagga tgcgttttta tcattttgaa
    11641 atctatttac ccacctcatt ccccttcttg atttcgttta agggaaaagt ggtctggaac
    11701 ggggtcctta cctgttttcg accagggatg acaacgtaaa atccgagcca agcccatcaa
    11761 taccccctta aaaccatgtt tttcaatagc ctgaatcatg tagttggaac aagtcagctc
    11821 aaagcgacaa gagggtggaa agactggtga gataaaacgt tggtaaaagc gcacaagcgc
    11881 tattaaaatt cgtttcatta tttcttagtt acagccatgg tgtgtagttg gctgatttct
    11941 tttttattaa gacgacggga ttctcctgga cggagtcctg tcaagtctag gtgtccgaaa
    12001 cgagtccgag acaacttatc tacttggaga ccaacagctt caaacatctt tttaacctga
    12061 tggttacgcc cttcatggat ggtcaactgc accacagagc gatttttgac tgggtccact
    12121 ttgagaattt cataaacagc tggcttggtt ttcttaccat caatctcaag accacgggtc
    12181 aaggggcgaa gattgtcctt attggccaca cctttaacac gcgcgacata aaccttgtca
    12241 atctcattac gagggtgaat catctcgtct gtaaagtccc catcattggt caaaatcaag
    12301 actcctgatg tatcccagtc caaacgaccc acagggtaaa tacgctcttt gacattgggc
    12361 aagaggtcga caaccgtctt gcgaccctta tcatctgtca cactggaaat gacaccgcgt
    12421 ggtttgttaa gcagatagta gaccttttct tcgttgtaga taggttgacc ttcaacttcg
    12481 accttgtcgc ctgacttgat agtggttgct agttcacgca ccacttggcc gttaaccgtc
    12541 accaagcctt gcttgatcag ctcttctgct tttctcctac tggccacacc tgcgtgggca
    12601 atatacttat tgattctcat cttcttctat cctttcacca aataattggc tttcttgggc
    12661 ttgaatctca agctcatcaa tcactggtaa ttcttctaaa tggtttatcc ccatgtaatc
    12721 taggaaataa tccgtagtca catagaggtt ggggcgcccc aatacttcct ttttcccgtc
    12781 ttcctttatc aggtcaaaag cctgcaactt tgccaaggct ccactcgagt taactccacg
    12841 gatggcatca atttctatcc gcgtaatcgg ctgtttgtag gcaataatgg acaaggtctc
    12901 aagggcagcc cgagacaagc tctggttgat aggcgcctta gagtattcct tcaaaatctc
    12961 tgcaaattga ggcttggtca ccaatctata agcaccactt gtctcaatca aagccaaact
    13021 ggaatctggg tccttttcat acttctgggc taattttcct aaactttgct ggatgcctgt
    13081 cggtggcaga gagaggagtt cagctaactg gcggacccga atcccatctt cacccgctac
    13141 aaacaagagc gcttctattt ttgctaaagt actcatcttt cctctctatc aagtctagct
    13201 ttgaggcact tgactttctt ccttcttttc catgagatag atatctccaa aactctcctc
    13261 ttgcacgagg atcagctcct gggttttgat taactctagg gttgccaaaa agagggtgat
    13321 gacctcttgg acattctggg cttccttgaa caaatcctgc aagcgcaatt gatctcgtcc
    13381 aatcaaggac tctttcacga taatcatcat gtcctcaatc ttatactcat cccgcaagat
    13441 cgtcgtgtga ttttgtgcaa actcctcttt tttcttggct agtatatttg aaaaagccaa
    13501 aaagaggtca atggtcgtct tgtcatgcac aagctccgca tcttcgtaaa tcaactctgt
    13561 cggcgctttg gaataatact gggcccgttc ttggtgcttg gcttccaagt gctcacccaa
    13621 gagcttgaac ttgcgatatt cttcgatttg agagaggagg tcctgctcca ggtcatcccc
    13681 caagtctgtc acttctgcta ccttcggaag gagtttacga ctcttaatca gcatgagctg
    13741 actagccatg accatgtact cacccgtcac ttccagacgc atggcctgca gggttgagac
    13801 ataggctaga tactgttcga tgacttccgt aatgggcaca tcgtagatat ccatctggta
    13861 cttagaaacc agatgcaaga gtaagtccag gggtccttca aaatctttta atttaatatc
    13921 cattatctat atttttctaa ggtcaggact gtttttaatc ctaatttttt tgcaatttcg
    13981 tacaaatcga ccttgttttc tatttgtctt agaataaact gttcacgtaa gacttgagct
    14041 gataaggatg gaaaaccttt ctccttgaca aaagattcta actgacgaaa ggcccactga
    14101 cgagaatagg gtttctctcc tctttcaaaa agataggtct gccccatcaa gggttccaat
    14161 tctgaaagca aggccgtggg aatggtgaca atcctctgtt gggaagcctt gctgattcgc
    14221 agcacctgaa aatccagatt gatgtccgca accttgatgg ctaaaatctc actgggcaag
    14281 agccccattt ctaggattaa gagcgctagc aagcggccct ctggatggtc gctttcctgc
    14341 caaaaagagt ctaggtatag aatctctggc ttttccgtct tcttttcagc ttgtttggct
    14401 aattccaagc ggtaaaagct gtccacctct cctttttgat agagaaagta gagaaattgg
    14461 ttacaggccg aaatctttcg cttctgggcg ctgattttta gattggctag ctgggcttgg
    14521 taaatcttga gactggtctc agaaatccgc tcacctacca tgtctaaaaa ttgctccaaa
    14581 tcatacttat aggactgctt ggaattgaca gacaagccct gcttttcctc taaaaaggct
    14641 gaaatcctgt ctctcattgg catcgaatct catattcctt actaaagtca tgcaagaggg
    14701 cattaacggc cttgaggatg gacttgcgcg taataatccc ttggaaaata ccctctgcat
    14761 ccacaaccgg taagaaggac tcatctacta gcttgtgcaa gacctccgta atggtgaaat
    14821 caggcgaaac aaccgctacg tccgtttttg tcatatgaac gatatccgta tccgccatga
    14881 tttcttggct caagtcatgc tccatctgat aagccataat atctctgagt ccaatcgtcc
    14941 caacaaactg tttttcatct gtcacaacgg gaacacgggt ataggtcatc tgactgagca
    15001 agagggtcgc atgatccgca ttgtgggtat caatcaacac agctagattt ttagcagggg
    15061 tcaaaaaagt ttcctcctgc cccaacaaga aagtctcaaa ctccttggca atcatcggct
    15121 aaactccttg gacaaacctg gatacacctc gtgatctcgt gtcaaaaagt ccactttgaa
    15181 gtaactatca tcaatctcca cacgagcata gagacattct ctgatggtac ctcgtggttg
    15241 gctgatagaa cctggattta gaaagaggat cttgccttcc atccaagcac ttggcacatg
    15301 caagtgacca tagaggcaga tagcggcctc ttcctcctga gcccagtagt ccaacttttg
    15361 aaagttgaaa ttgatgtcaa acaagtgacc atgagtttgg ataatcttgg tcgaaccaag
    15421 ctcagtcacc agacgttctg ggtagccggc gtagaagtcc atgttccctt taacaacgcg
    15481 gatgccctcc caaagtggag aatccggacg tagttcagaa tcgccgttat gaaaaacggc
    15541 atcgactttg cccacatagc gatcacggac ttcttccaca atcaagctat cgccatggga
    15601 atcgctcatt acaatgatgg tttgctttgc catgatggaa atacctccaa aagtttctta
    15661 acggctaagg cacggtgaga ttgactattt ttttcttcca gggttaattc agctgatgac
    15721 tcacctgtct ctcctacaag gaagagggga tcatagccaa agccattttc acccttaggt
    15781 tcaaagttaa tataacctga ccagtctgct tcaacaacta aactttcctt atttgggctg
    15841 gctacgacta gggttgtgtg gaactgagcc gagcggtcct tgagttcaaa gaccatggcc
    15901 aattcgtgca agagtttggc attattttca cggtcagttg ctcccacacc tgcgaaacga
    15961 gctgaccaga cgcctggtaa gccaccaagg acatcgactt tgagaccaga atcatctgcc
    16021 aaaaccatct tgcccgttaa ttgagaaatg gtttctgcct taaggcgggc attttcttca
    16081 aaggtcatac ctgtttctgc tacttcaggc aggtcagggt agtcattaag attttccaca
    16141 tcgtagccta acttatcaaa gatagctcgg aattccttgg ttttaccttc gttacgagtc
    16201 gcaatcaaca aggtttctct gaccttgctt gtctcaaaga aatcatgaac attgacccct
    16261 tctttaggca attctacata caagagttgc ccattttcaa ccaagagcag ggccccatga
    16321 cgttgaataa cttccaagtt tcgtcccatt tcttgattaa gcatgtctac cacaaaggag
    16381 aacaaacggt aggtagaacc atagcgtaaa acagtaactg aaagcgggaa accatactct
    16441 tcatctccaa atattttgga gaattcaaac agaggaaaat ctgtcttata gtcgctcaaa
    16501 ctgttaacgc caccaaaaat actataagac ccaacatacc agtcctggtc atccttatat
    16561 tcataaattt tatttgtcat aattctacat gctccacatg aatctctttt tccagccatt
    16621 cttcaccaat ttgtgcaaaa ctttggctac tggctgttgt gtaaaaacgg tgatggagtg
    16681 gtccagcatc gcgaccacga ttgatttcaa aataattgag taagactgag atatcccgta
    16741 cgcactctgc cccactatcg atgagctgaa cctttggccc catcacattt tggataatag
    16801 ggcgaaggag tggataatga gtacagccca aaatcaggct atccaccttt ccaaccaagg
    16861 gacgcagggt ttcatagacc accttcttgg taacactggt tgacagggca cctgactcaa
    16921 ccaagggagc aaacttggga caggccaagc tctccacctg taagtcggga tccagatcat
    16981 ggattttctg acggtatatg tctgattgta ccgtcatggg cgttccaatc actccgattt
    17041 tcccaccttg actggacttg atggctgccg aagctcctgg caaaattaca cccaagacag
    17101 gaatatctag ttgagccttg atttcttccc agacgaccgc agtcgcagtg ttacaagcaa
    17161 tgacaatcat tttgacatcc ttggtcaaga gaaagttgac cagctgccaa gtatattcac
    17221 gaatttgctc agcaggacgg gggccatagg gcgcccgcgc cgaatctcca atatagacga
    17281 tttcttcatg gggaagctgg cgcatgagct cgcgcacaac ggtcaagccc ccgacacccg
    17341 aatccaaaaa accaattggt cgattatcca tacagtcttc tttctagtct tttttctgat
    17401 tgatcgttca ttatgattcc ttgtactgaa cgaactgacc gacagcttga tagaatgtca
    17461 actacctctc tcttaatcat aatcaaatat caatagaatg caaggaaaaa gtctcatccc
    17521 taaaccataa ccaacatagt gagaagtctg gaactttcat cccagacttt tccttattta
    17581 ttttttcttt ttattgttag caagggctgc cttttgttgg cggatgattt ggtggtaaac
    17641 ttgttgtacc ttagcttcgc ttggtttttg accatttgca ctcaaaagag tacgaactgc
    17701 ttcagcattc aaacgtgggt tgtcagcaaa ttctttttcg atttgcttac gaaccaagta
    17761 cattcctcca agagctcctc ctagaaaagc tagcacaatc aatacaattg ctaaaagtaa
    17821 atccatcatt tttctcctgt ttctttttga gtccctttca ttataccaca aactagccac
    17881 cctgaccagt ttgttctaca ctttcaagga tggaatagac agcaaaaaga accctgcttt
    17941 tctgaaaaag aggtctcctt gctaagttta tactcaatga aaatcaaaga gcaaactagg
    18001 aagctagcca caggttgctc aaagcaccgc tttgaggtta cagataaaac tgacacggtt
    18061 tgaagagatt ttcgaagagt attaatttac ataaatagcc agtgtttgat agggtttgag
    18121 tagaattttc tcagacactt ctgcatcttc atagtttgaa atcaaaatct gtccattttg
    18181 gtagactgct ggcaagtcga tttccacttc tttggcataa aagttattga gcactagtaa
    18241 cttttgatcc tcaaactggc gttcaaaagc gtagacttgt ttgctatctt caaaggctgg
    18301 tttgtaattt ccttctgaaa tgattggcat ttccttacgc agtcgaatca agtcttgata
    18361 gaaggtgaaa atcagacctt tgatttcatt ttctacattg atatctttat aggatttacc
    18421 aactttcaac catggagtgc ctgttgaaaa tcctgcattt tcagaagtat cccactgcat
    18481 gggaatacgt gaattatcac gcgacttagc ttgaataatc tggaaggctt cttgctgact
    18541 ctttccttct tctaagagca tctgataggc attaagcgat tcgacatcca cataatcagc
    18601 catagaatca tagtctgggt caatcatccc gatttcctca cccatgtaga tataaggtgt
    18661 cccacgtgac aggtgaatgc tggctgctag catggtagct ccttccttgc ggaagttttg
    18721 aatatcgaca aaacggttca aggcacgtgg ttgatcgtga ttgttccaaa agagggcact
    18781 ccaaccgtct ttatcactca tttccttacc ccaactatgg taaagactct tcaactcttc
    18841 aaaatcaaag ggagccaagg tccacttttg tccatcctta tagtccacct tgaggtgatg
    18901 aaaattaaag gtcatggata attcctgacg atcaggcgac gaatagagga cacagttttc
    18961 catggtggta gaagacattt ccccaactgt cataaagcta tcgtcagatc caaaagtggc
    19021 ttggttcatc atacgcaaat agttatgaac gatgggtttg tctgtataag ctggcttccc
    19081 ttcattttca ggacagtcca ctgaaacctc gtccttaccg atcaaattga tcacatcaaa
    19141 tcggaaacct ttgacaccct tgtcgcgcca gaaattaaca accttgaaaa gctccttacg
    19201 gacattggaa ttgcgccagt taaggtcagc ctgggtctca tcaaataggt gaagatagta
    19261 tttcccagta tccccgaaag gcgcccatgc agaaccacca aacttagact gccaatctgt
    19321 tggttggtct tggatgaaga aaaagtcttg ataatactta tcaccagcta gggctttctg
    19381 aaaccattca tgctctgtcg aacaatgatt aagtaccatg tccagcataa agtcaatctt
    19441 gtgctcttta ccgacacaca ccatttcctc aaaatcagcc atatcaccaa aaagaggatc
    19501 cactgccata taatctgaaa tatcgtaacc attatcccgt tgagggcttg gatagaatgg
    19561 attgagccag accatatcca cacctagttt ggctaaatag ggaatttttt cgataatccc
    19621 acggaaatcc ccaataccgt tttcagtggt gtctttgtaa gattttggat agatttgata
    19681 gactactttt cctttatcaa gtgtcatctg tttctccttt tctgataaaa gggaggaagc
    19741 agtcttccgt ccctatttgt gctatttcaa ttatactcaa tgaaaatcaa agaacaaact
    19801 aggaagctag ccacaggttg ctcaaaacac tattttgagg ttgcagatag agctgacgtg
    19861 gtttgaagag attttcgaag agtattagat tcgtgtagcg accatgagag atgctccagc
    19921 ttggatcgtt gtcggataag ttccgggaat agtcgctgta taagcatctt ggttggtgat
    19981 gataacagga gtttctgtca ccagacctgc agccttaatg acatccatat caaaacgaat
    20041 cagttgctga ccaactgtaa cgtgatctcc ttggactaca agactttcaa aacctttgcc
    20101 atcaagacct actgtatcca taccgatgtg gatgagcaat tcaactccct cgtcagagac
    20161 aatgccgatg gcatgcttgg tagggaaaag aaccgtcact gtcccattaa ctggagaggt
    20221 caactcacct tggcttggtt caatgactag accttgcccc atgacacctg atgcaaaaat
    20281 aggatccgtc gcttgactca attctttcac ttggccagtt agtgggctga taatttctac
    20341 cgaagtaagt tctactggtt catggttcac aaattctgct tcttcttgag caacgaattc
    20401 tgcctgcaag ttcgtatcgc cctctgtttt tgtaaagaga ccagccttgc ggaagaagaa
    20461 agtcaagagc attggaacaa caatcgcaac tagcatagtt cctgcaaatg gcagcatgta
    20521 ttgaggttga atagagagaa tacctggcaa accaccgata ccaatagaag ccgcagttac
    20581 attaaaagta acggataaca tgcctgcaag ggctgaacca gtcatcccag caacaaatgg
    20641 ataaatatat tttacgttaa ccccaaaaag agctggttct gtaacaccga gataggctga
    20701 aatggttgca ggaagtgaaa cctgagcctc acgctcatca tggcgatgca tgaaataata
    20761 ggcaaacacg gctgagcctt gagcaatatt agaaagagca atcattggcc atagggcagt
    20821 gccaccagca tccgcaatca attgtgtatc aatggcattg gtcatatggt gcagacctgt
    20881 gatgacaaat ggagcgtaga gggcaccaaa aattgcaccg aagagccatt taactggacc
    20941 agttaaacct gccaagacaa ctgatgaaag tccttgtcca attgtccaac cgattggtcc
    21001 caaaacagta tgagccaaaa tcaaggctgg aatcaatgac aagaaaggta caaaaatcat
    21061 agaaatgact tctgggatat gcttgcgcca gaagatttca agataagaca gactcaaacc
    21121 tgcaagcaag gctgggataa cttgggcttg gtaaccgata cgattaacag taaaatagcc
    21181 aaaattccaa acccagtttg ccgcgatatc agctgctggc gttgaagcaa ccgcataggc
    21241 attgagcaac tgaggcgata ccaaacagat tccgagaaca attcccaaaa tttggctggt
    21301 tcccatctta cgagaaacag accaagtaat ccctactggt aagaactgga agatagcttc
    21361 accaggcaac cagaggaagt gattgacacc tgcccaaaac tgagaggatt ctgtgatggt
    21421 cttgccatcc aacatcgacc aatggacacc ttccaagaca ttacggaaac cgaggatcaa
    21481 tcctccgact atcaaggctg gaataatcgg agtaaaaatc tccgccagag tggtcataac
    21541 acgttggacc acgttttgat tactcttagc tgcagacttg gctgcttctt tggaaacacc
    21601 ctcaatacct gaaacggctg taaaatcatt ataaaagatg ggcacgtcat ttccaatgat
    21661 tacctgaaat tgacctgcat ttgtaaaagt tcctttaaca gctggaattg actcgatagc
    21721 tttaacatta gccttcttat catctcctaa aacaaagcgc atccgtgtcg cacagtgggt
    21781 gacggcagtc acattttctt tgcctccaat tgcctgaagc agatctttgg cttcttgttc
    21841 aaattttccc atttgattct ccttatactt gtcaaagcaa gtttgatttg ataagtatat
    21901 tgtaaacgat accaaacttg tctgcaactt gtttggtata aaaagggtgt ttttttgtaa
    21961 attttctagc acttgtatgg taaaatagat acaggtgttc attaaactag actaaaaacc
    22021 tatttaagca ggcaaaatga agaaatacca acaattattt aagcaaatcc aagaaaccat
    22081 tcaaaacgag acttacgctg tcggagattt ccttcctagc gagcacgacc ttatggagca
    22141 atatcaagtg agtcgtgata ccgtccgaaa ggccctgtct ctcctccaag aggaaggatt
    22201 gatcaaaaag ataagagggc aaggttctca agtcgtcaaa gaagaaaccg tcaatttccc
    22261 tgtatccaac ctaaccagct accaagaact agttaaagaa cttggactgc gctctaaaac
    22321 caacgtggtc agtctggaca agattattat tgataaaaaa tcctcactga taaccggttt
    22381 cccagagttt cggatggttt ggaaggtggt ccgccagcgt gtggtggatg atctggtatc
    22441 cgttctggat acagactatc tggatatgga actcatccca aatctcactc gccaaattgc
    22501 tgagcagtct atctattctt atatagaaaa tggcctcaaa ctccttattg attatgctca
    22561 gaaggaaatc accattgacc actcaagcga ccgagacaag attctcatgg acattggcaa
    22621 agacccttat gtcgtttcga ttaaatcaaa agtctatctc caagacggac gccaatttca
    22681 gtttaccgaa agtcgccata agttagaaaa atttagattt gtagattttg caaaacgcaa
    22741 gaaataaaag actgagacac caatctcagc ctttttcttt atctcaaaac taattcatca
    22801 ctgaccagac tttggccact atttttctgg aagagatgca taaggtcttc aactgtcaga
    22861 tgctttttct cttctccctt gacatccacc actatcttgc cttgatagag cataatgaga
    22921 cgattgccgt actcaatcgc atgattcata tcatgggtaa tcatcaaagt cgtcaactga
    22981 tggtgctcca caatcttttg cgtcaagtcc atcaccattt gactggtttt cgggtcaagt
    23041 gccgcagtat gttcatccaa aagcaaaagt ttgggtttca ccagagctgc catgactagg
    23101 gtcaaagcct gtctttgtcc tcctgagaga tattgagtat ctacttttaa gcggttttca
    23161 agaccaatat tcaactcctt caaggcctct tggaactgga ttctatcctt ctccttcacg
    23221 ccccaaccaa gccctctctt ttgcccacgt ctcaaggcaa tagccatatt ctcctcaatc
    23281 gtcaaacgag aagctgttcc catcttagga gcttgaaaaa cacgggcaat atccttggct
    23341 ctctttctga cactcagatt tttaatggat ttccctgcca gtagaaggtc cccttcgtcc
    23401 atcgatagat tgccagccaa gatattcatc aaagtggatt ttcctgctcc atttccacca
    23461 atcacagaga taaaatctcc ctcttcaacc tctaaatcta atcctttgag gacatggttc
    23521 tcattgaccg tccctgcttc aaatgtcttg tgaatatttt caagtgttaa caaacttgcc
    23581 ataactttct cctcctattc atttctcaat ttcagaccac gaatctttaa tctcttttgc
    23641 aattctggtg caaataaaac caaagcgagc aagattgcat taaagagacg taccaagttt
    23701 tgatctagat ttggaatttc atagatattt aaaataatca aacggtaaac aatagcaccg
    23761 attccgatgg ataataagcg gcctccaatg gtcaagtcgt gtatcaagac ttccgcaata
    23821 atcaccgaac tcaaaccaac aacaatggtt cctgtcccag aagtcacatc tgaaaatcca
    23881 tcattttggg caaacaagga accacatagg gcaatcaaac cgtttgaaat catgtaacca
    23941 acaatcttca tggtgtctac attgactcca ttggcctcac tcatcgggat attatcccca
    24001 gtcgaacgca agaccaaacc aatctctgtt ttcatcaaaa gggtcaagac caaacaagta
    24061 agagacagac taaactaagt gagaaaacag ctccttcatt tgtcagaccc aagctcgcca
    24121 actgtttaaa gacggttgca gaatctccca aggaaagatt gagtacactg cccatgattt
    24181 taatattgat tgaatagagc cctgtcaagg tcacaatccc tgtcaagaga gctgggattt
    24241 tcatcttggt gtggagcatt cctgatacaa gacctgctac catacctgcc agcaaagcaa
    24301 gtaaaatcgc aagccaagga tttgcccctg cctgtatctg tgatacaacg actgctgccc
    24361 ccagaggaaa ggctccctca gcagtcatat ccgcaatatc caaaatacgg aaagtcaagt
    24421 agaccccaat cgccacaatc gaccacaaca aaccttctga taaactagat aacacaaaat
    24481 tcatcttaat cctccttatt ccttgccttc taacttacta atatcaattc ccaatgcatc
    24541 tgccatttct tgattagtat gcaattccag tttttcaggc aactcaactg ctatattttc
    24601 tggtttctca ccttttaaaa cacgaatcag catgcgagct gtctgtcttc ccaattcttc
    24661 ataattggtt ccgtagttat acaagtcacc aacagcaatc atttctgttg aaccaccgaa
    24721 tactggaacc ttgtgtttaa tagaaacctg cttaactgtt tccatggttg acgaaatgat
    24781 attatcagtt gggacaaaaa ccatatccac ctctgccatc aaactctctg ctgccgcctt
    24841 gatgttatta ctgtccaaga ttgttttttc aacaacggta aagccttttt ctttcagaag
    24901 tcgcttagct tcatcctttt aaacaaccga attgggctcg ctctgagtat agaggattcc
    24961 aatcatttta gcctttagca acactttctt tatcaaattg atttgggttg aaatggcatc
    25021 tgatgactga tcgcttgtcc cagttacatt gcccccagga tgttctcttg actcaaccaa
    25081 cttggcactg actgggtctg ttacagctga aaagataact ggtgtcgttt gcgtcgtatt
    25141 ggccaagctc tgagcagaag gccttgcgat ggctagaacg acatcgctgg attctgctag
    25201 ttgctgggaa atcgttttta gatttctttg ttctccctgt gcattttgca aatcaatctc
    25261 aatattttcc ccctcaacat agccttgttt ggccagctca tccacaaatc cttctctagt
    25321 agcgtctaag gactggtggg taataaactg cgaaataccg atacgaaaga catctctttt
    25381 cttatctgcc ttcaactgat gcaaactgat cagagaagtc aagaggatcc ccactaccaa
    25441 gagggggcta gtgattttcg aacaactttc ataatgaaac tccttctcag aaaagataaa
    25501 acaaaaaacc gcctagatac tatctaaacg gatacttgaa ataacctaac aagttacaac
    25561 ttagctagtc catttagata ttcatctata tggaccacaa tcaaaaatct tagccacata
    25621 gatacaaaca aattgcttga actgtttgca tccatggtga catgtctaca aacactatct
    25681 aaagtagcta aagtaaaagt tttgattcat agttacggct tgtctcttat tcatttcttt
    25741 ttctgctttc ttttttgatg cttcctatcc taccaccttt ttcgtcacct gtcaa
//
LOCUS       DAVKGC010000016        25190 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKGC010000016 DAVKGC010000000
VERSION     DAVKGC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.016"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(124..1794)
                     /locus_tag="VW049_000549"
     CDS             complement(124..1794)
                     /locus_tag="VW049_000549"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9431718.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2024..2713
                     /gene="coaB"
                     /locus_tag="VW049_000550"
     CDS             2024..2713
                     /gene="coaB"
                     /locus_tag="VW049_000550"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9431719.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2725..3276
                     /gene="coaC"
                     /locus_tag="VW049_000551"
     CDS             2725..3276
                     /gene="coaC"
                     /locus_tag="VW049_000551"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9431720.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            3260..3823
                     /locus_tag="VW049_000552"
     CDS             3260..3823
                     /locus_tag="VW049_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9431721.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            4105..4632
                     /locus_tag="VW049_000553"
     CDS             4105..4632
                     /locus_tag="VW049_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9431722.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            4644..5159
                     /locus_tag="VW049_000554"
     CDS             4644..5159
                     /locus_tag="VW049_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9431723.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(5156..5623)
                     /locus_tag="VW049_000555"
     CDS             complement(5156..5623)
                     /locus_tag="VW049_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9431724.1"
                     /translation="MFRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(5697..6185)
                     /locus_tag="VW049_000556"
     CDS             complement(5697..6185)
                     /locus_tag="VW049_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9431725.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(6151..6714)
                     /locus_tag="VW049_000557"
     CDS             complement(6151..6714)
                     /locus_tag="VW049_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9431726.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(6724..8712)
                     /gene="uvrB"
                     /locus_tag="VW049_000558"
     CDS             complement(6724..8712)
                     /gene="uvrB"
                     /locus_tag="VW049_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9431727.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(8789..9742)
                     /locus_tag="VW049_000559"
     CDS             complement(8789..9742)
                     /locus_tag="VW049_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9431728.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            9915..12080
                     /locus_tag="VW049_000560"
     CDS             9915..12080
                     /locus_tag="VW049_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9431729.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            12080..12820
                     /locus_tag="VW049_000561"
     CDS             12080..12820
                     /locus_tag="VW049_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9431730.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            12969..14456
                     /locus_tag="VW049_000562"
     CDS             12969..14456
                     /locus_tag="VW049_000562"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9431731.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(14502..15779)
                     /gene="ftsY"
                     /locus_tag="VW049_000563"
     CDS             complement(14502..15779)
                     /gene="ftsY"
                     /locus_tag="VW049_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9431732.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETIE
                     ENNSEVLEPERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(15783..16601)
                     /locus_tag="VW049_000564"
     CDS             complement(15783..16601)
                     /locus_tag="VW049_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9431733.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(16601..17395)
                     /locus_tag="VW049_000565"
     CDS             complement(16601..17395)
                     /locus_tag="VW049_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9431734.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            complement(17392..20931)
                     /gene="smc"
                     /locus_tag="VW049_000566"
     CDS             complement(17392..20931)
                     /gene="smc"
                     /locus_tag="VW049_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9431735.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKASQQKDELETAKVQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRVGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDQMAALTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQESLATIASEKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEEVAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(20922..21620)
                     /locus_tag="VW049_000567"
     CDS             complement(20922..21620)
                     /locus_tag="VW049_000567"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9431736.1"
                     /translation="MKELQTVLKNHFEIEFADKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(21803..21874)
                     /locus_tag="VW049_000568"
     tRNA            complement(21803..21874)
                     /locus_tag="VW049_000568"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21839..21841),aa:Arg,seq:ccg)
     gene            complement(21938..22924)
                     /locus_tag="VW049_000569"
     CDS             complement(21938..22924)
                     /locus_tag="VW049_000569"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9431737.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(23090..24406)
                     /locus_tag="VW049_000570"
     CDS             complement(23090..24406)
                     /locus_tag="VW049_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9431738.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYQRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGAQLKKWSIRIPQNASFYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(24393..>25190)
                     /locus_tag="VW049_000571"
     CDS             complement(24393..>25190)
                     /locus_tag="VW049_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9431739.1"
                     /translation="SVNYDSGVPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETL
                     RKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPDYRGEKGSVSTQ
                     YANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKE
                     LNIHAEEAKIRLRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLL
                     EDYLRGSYDEVETLETLKKAFELTNNEQKDQAVADDNEGDENDDADY"
BASE COUNT         7335 a         5630 c         4535 g         7690 t
ORIGIN      
        1 gaagctgacg tagtttgaag agattttcga agagtataaa tcttattgat gaactgcttg
       61 cagtctgaga aaaaatgagc ttggatatta tttccaaact cacttaaagt caatttcaat
      121 ccactagaac aagcctagta cagttccatc gctttcaaca tccatgttga gagctgctgg
      181 acgttttgga agacctggca tggtcataac atcaccagtt aaggcaacga tgaagcctgc
      241 acctaatttt ggtaccaatt cacgaatggt aatttcaaag ttttctggtg ctccaagcgc
      301 atttggattg tctgagaaac tgtattgagt tttagccata cagattggca atttgtccca
      361 accgttttga acgatttgag caatttgtgt ttgagctttc ttctcaaagt tcactttgct
      421 accacgatag atttcagtga caattttttc aatcttttct tggacagaaa ggtcgttatc
      481 atacaaacgt ttatagttag ctggattttc agcaattgtc ttaacaactg tttcggcaag
      541 tgctactcca ccttctgctc catcagccca gacactagcc aattcaactg gtacatcgat
      601 tgaggcacag agttctttta agactgcaat ttcagcttct gtatcagata caaattcgtt
      661 aatagctaca actgctggaa taccgaactt acggatattt tcaacgtggc gtttcaagtt
      721 agcaaaacct gcacgaactg cctctacatt ttcttcagtc agagcgtctt tagccacacc
      781 accattcatc ttaagggcac gaagggttgc gacaataaca actgcatctg gagatgttgg
      841 caagtttggt gtcttgatat caaggaattt ctcagcacca aggtccgcac caaaaccagc
      901 ttcagtaaca gtgtaatcag ccaagtgaag ggctgttgtc gtcgccaaaa cagagttaca
      961 gccatgagcg atattggcaa atggaccacc gtgtacaaag gcaggtgtac cgtaaattgt
     1021 ctgaaccaag tttggcttaa tagcatcctt caaaatcaaa gccaaggcac cctcaacctg
     1081 caaatcacct acagaaacag gcgtacggtc atagcgataa ccaataacga tattcgccaa
     1141 acgacgtttc aagtcctcga tgtccgttgc caagcaaaga attgccatga tttctgaagc
     1201 aactgtaata tcaaaaccgt cctcacgtgg aataccgttt agaggaccac caagaccaac
     1261 agtcacatgg cggagcgcac ggtcgttcaa gtccacaacg cgtttccaga ggatacgacg
     1321 ttgatcaatt cccagctcat tcccttggtg caagtggttg tcaatcaagg cagaaagggc
     1381 attgttggca gttgtaatag catgcatatc tccagtaaag tggaggttga tgtcttccat
     1441 tggcagaact tgtgcatacc caccaccagc agcaccaccc ttgatcccca tgactggacc
     1501 aagagacggt tcgcggatag caatcatggt ttttttgcca atcttgttca aggcatccgc
     1561 aagaccaatg gtaagcgtcg actttccttc acctgcaggt gttgggttga tggcagtaac
     1621 caagatcaat ttaccgactg gattgctctc aactgcacga attttatcaa agctgagttt
     1681 agccttgtac tttccgtaca actccaaatc gtcgtaagaa ataccaagtt tctctacaac
     1741 atcaacaatt ggcttcaact caatactctg tgcgatttca atatctgttt tcattcaaaa
     1801 ttcctctaac ctcttatatg ataattcatt atatcacaaa acaagatttt taacatccta
     1861 aaactctcta aacgttcgta aatatctctg tttttaaggc ttttagagtc ctttcttaaa
     1921 ttttatatgg ctttatagtt tgaaactata gtaaatcttc gtttttacca aaaatttatc
     1981 actttcattt tacttaccgc ttatttttgt gtacaatagt gctatgaaaa ttttagttac
     2041 atcgggcggt accagtgaag ctatcgatag cgtccgctct atcactaacc attctacagg
     2101 tcacttgggg aaaattatca cagagacttt gctttctgca gggcatgaag tttgtttaat
     2161 tacgacaaaa cgagctctga agccagagcc tcatcctaac ctaagtattc gagaaattac
     2221 caataccaag gaccttctaa tagaaatgca agaacgtgtt caggattatc aggtcttgat
     2281 ccactcaatg gctgtttctg actacactcc tgtttatatg acagggcttg aggaagttca
     2341 ggctagctcc aatctaaaag aatttttaag caagcagaat catcaggcca agatttcttc
     2401 aactgatgag gttcaggttt tgttccttaa aaagacaccc aaaatcatat ccctagtcaa
     2461 ggaatggaat cctactattc atctgattgg tttcaaactg ctggttgatg ttaccgaaga
     2521 tcatctggtt gacattgcac gaaaaagtct tatcaagaat caagcagatt taatcatcgc
     2581 gaatgacctg actcaaattt cagcaggtca gcaccgagct atatttgttg agaaaaatca
     2641 gcttcaaaca gtccagacta aagaagaaat tgcagaactc ctccttgaaa aaattcaagc
     2701 ctatcattct tagaaaggaa aactatggca aacattctct tggctgtaac gggttcaatc
     2761 gcctcttata agtcggcaga tttagtcagt tctctaaaaa aacaaggcca tcaagtcact
     2821 gtcttaatga ctcaggctgc tacagagttt atccaacctt tgacactaca ggtactctca
     2881 cagaatcctg tccacttgga tgtcatgaag gaaccctatc ctgatcaggt caatcatatc
     2941 gaacttggaa aaaaagcaga tttatttatc gtggtacctg caactgctaa cactattgca
     3001 aaactagctc acggatttgc ggacaacatg gtaaccagta cagctctagc cctaccaagt
     3061 catattccca aactaatagc tcctgctatg aatacaaaaa tgtatgacca tccagtaact
     3121 cagaataatc tgaaaacatt agaaacctac ggctatcagc tgattgctcc taaggaatct
     3181 ctactagctt gtggagacca cggacgagga gctttagctg acctcacaat tattttagaa
     3241 agaataaagg aaactatcga tgaaaaaacg ctctaatatt gcacccattg ctatcttttt
     3301 tgctaccatg ctcgtgatac actttctgag ctcacttatc tttaaccttt ttccatttcc
     3361 aatcaaaccg accattgttc atattcctgt cattattgcc agcattattt atggtccacg
     3421 agttggggtt acacttggat ttttgatggg attacttagc ttgacggtta acacgattac
     3481 gattctaccg acaagctacc tcttctctcc cttcgtacca aacggaaaca tctactcagc
     3541 tatcattgcc atcgtcccac gtattttgat tggtttaact ccttacttag tctataaact
     3601 gatgaaaaac aagactggtc tgattttagc tggagccctt ggttccttga caaatactat
     3661 ctttgtcctt ggaggaatct tcttcctatt tggaaatgtt tataatggaa atatccaact
     3721 tcttctggca accgttatct caacaaattc aattgctgaa ttggtcattt ctgcaattct
     3781 aaccctagcc attgttccac gactacaaac cttgaaaaaa taaaaacaat ctccgctttc
     3841 aaacctgaag gtggagattt tgctctatac agtttctttt taatgaaatc tttactatag
     3901 taaaacgaaa taagaacagg acaaatcgat caggacagtc aaatcgattt ctaacaatgt
     3961 tttagaagta gaggtggact attctagttt caatctacta taacttttaa ctaaaaaatg
     4021 atctataaac ccaagtaaat tcttgcttta ttgtctggtt tattgtacca tactagtgta
     4081 tatgcagtta aaaaggagat tcttgtgaat acacggaaaa agacacaatt tatgacaatg
     4141 acagcccttt taacggctat tgcgattttg attccaattg ttatgccttt caagattgtc
     4201 attccacctg cttcctatac tttggggagc cacatcgcta tttttatagc catgttcttg
     4261 tcgcccttga tggcagtttt tgtcatccta gcctctagtt ttggattttt gatggctggc
     4321 tatcccatgg ttatcgtttt tcgggctttt tcccatatat cttttggtgc tttaggagct
     4381 ctttacctac aaaaattccc cgatacccta gataaaccaa aatcttcctg ggttttcaat
     4441 tttgttttgg ctgttgttca tgcccttgct gaagtattag cctgtgtcct tttttacgca
     4501 acttcgggta ccaatgtaga aaacatgttc tatgttctat ttgtactagt tggatttggc
     4561 acaattatcc atagtatggt agactataca ttagcactag ctgtctataa agtgcttcga
     4621 aaacgccgtt aaaaggaaaa gctatgacaa aagatcgcaa acaagccctg ctccaactct
     4681 tgaaagaagc tcctaaagcc cttaatggcc aaagtttggc ggaacatttt catgtcacac
     4741 gtcaggtcat tgtgcaggac attgcaattt tgagagccga tggcgctcct atcctatcca
     4801 ccaatcgtgg ctacatctat aagcaaattg ataccaatcc ttatgttcac aaacttttca
     4861 aagtgaaaca tgaagttgaa gaaatcggtc aggaactcct tgctattgtc gataatggtg
     4921 ggcatgtcca gaataccttg attgaccatc ccgtttatgg agaaattgaa accttgctta
     4981 aactgtcttg tcgcagagat gtgcaacatt ttctagaaca agtcgagcat tctgacttta
     5041 gacctctatc tgaattgaca gatggcatcc attaccacct agtcgaagcc gaaacacaac
     5101 aagacctcca ctatatcgag gaggccttgg atcagctagg ttatttagta aaagactaga
     5161 agattttctt ttcccaatcg tcttctgtac ggcgaggata gaaaaactct gacttgtcgg
     5221 gagcttccat catttccatc aatggtagca tatcataggc cagatttaag tttggaatct
     5281 ggtctttttg cacccaagaa acttctccct cttctgaaga ttgaagggta ccagagaact
     5341 cagtcgcctt ataacaaatg acaatatagc gcccacctgt atctagtggc caatttttaa
     5401 tgccgacaag ttgaggattt tggatagtca accctgtttc ttcgtagatt tcacgaatga
     5461 cagactccgc aaaagcctca tcattttcta catgacctcc aggaaaggca taaccagacc
     5521 agcgattgtt ttcaggggcg cgatactgca tcaccacgcg ctgagtttcg aggtcttcaa
     5581 tcagacagat atttgttaaa attgttaatt gggaacggaa cataaattta ctcgctttct
     5641 aatttattaa attttcagac ttactaccag agatattatc ttttactttt ttaaaattat
     5701 ttagaataga acaccattcg tttttccttt ataggctttc caagttcaag tattttttct
     5761 tgtccatcaa aagtaaaacc cattttttga tagaaagcaa tggcgcgctt gttatctttc
     5821 aatacccata agaaaatttc agaaaaatga ttaagagcag tcaaagctgc tttcattaac
     5881 ttttgtgcga taccttttcc ataatagtct tttaaaacat ataaagcaat aatttcacca
     5941 gcttgaatag tctcatcacg aaagttacca taactgataa aaccaactac cttcagatca
     6001 cctatcgcaa tcaatgtatt ttctggatac ttttgactaa agaatcgaca tctttctaat
     6061 gtcattgtct cctgaaattc tgcaggcaaa aggtcatcat aagcctctct ccacgtttgc
     6121 cagtgaacga gggatttacc ttctatctct tcaggagttt ccattgattt gataataacc
     6181 gtcatttatt ttctcccagt cttctctcaa aataccatat ttaatactat caaaatattt
     6241 accttgataa taacgaactt ttggaatatg agcttctttt ttcattctta atttttcagc
     6301 aagtttcatc ataccaagat ttcctgacca agttgtcaaa cccagatgct ccaactccaa
     6361 gtaatcctga aacgtcctat ctatccactg caacatagca actttcccaa taccagtgtt
     6421 ccagaatttt ttatcataaa taccaattcc caattccatc catcttgttt ctttacatac
     6481 ccaataacgc gaaacagtcc caactagttt atcatcaaca aaaataccaa ggcgatttga
     6541 gttgcttaaa atggattctg atttttgtag ttcgaattct ttaaaatttg aaaaatactg
     6601 ataatcatca taatagggag catcatactg tttccaaact ggattaagct gtgaataagc
     6661 catttcccat aaagacatca aatcttcttt ctttaacttt cttaaataaa tcatactatt
     6721 cccctaatcc aaggccttga cttccaacat catatcacgg atctgagccg ccagttcaaa
     6781 gtcaagcact tcgacggctt cttgcatttg tttttctagt ttcttgacga gttctttgcg
     6841 ctcttgtttg ttgaggctat tgatgtcgac ttccttgtcc tcttccttag caactgcctt
     6901 ggtcacggca atcaggtcac ggatttcttt cttgattgtc tgtggtacga taccatgctc
     6961 ttcattatag gccatctgaa ttttccgacg gcgggcagtt tcatcgatag cacgttgcat
     7021 agactgggta accgtatctg catacatgat gacatgacct tcactgttac gggcggcacg
     7081 cccaatggtc tggatgagtc cacgctcgtt acgaaggaaa ccttccttgt cagcatcgag
     7141 aatggcaacc aagctaacct caggaacgtc aatcccttca cgaaggagat tgattccaac
     7201 caagacatca aagacaccca agcggagatc acggataatc tccgtccgtt ccaaggtctt
     7261 gatatccgag tgcatgtact tgaccttgat gcccatttcc ttgaaatagt cggtcaagtc
     7321 ctctgccatt ttcttggtca aggtggtgat aaaggttcgt tcattctttt caacacgggc
     7381 attgatttca cctaagagat catcaatctg tcccatagtc ggacggactt ccacctcagg
     7441 atccaaaagc cctgtcggtc gaatgatttg ctcaatcacc gtctcagtct gttcattttc
     7501 atagtcacca ggtgtcgctg atacgtaaac aatctgatgc acatgacttt caaactcctc
     7561 acgacggaga ggacgattgt ccaaggcaga cggtaaacgg aaaccataat ttactagcat
     7621 ttccttacgc gaacggtctc cattgtacat gccttttatt tgtcccatag tcatatgact
     7681 ctcgtcaatc ataatcaaaa aatcatctgg gaaaaagtcg agaagcgtat aaggaggctc
     7741 accttcacta cgtccatcca tgtggcgaga ataattttca accccatttg tatagcccat
     7801 ctcgcgcagc atttcgatat cgtactctgt ccgttgtttc aagcgctggg cttcaagcaa
     7861 tttaccttcc ttctcaaaga cagctaactg ctcttctaat tctgcttgaa tcttagcaac
     7921 ggcaacttcc atatggtcgt cattggtcac aaagtgagta gctgggaaaa tcgccaaatg
     7981 atccacctct cccaatacct gacctgtcag agcctcaacc tcacggatac ggtcaatttc
     8041 gtctccaaaa aattctactc gaaaggcatg ttcatctcgg gaagctggga aaatctctac
     8101 cacatcccca cgaacgcgaa atcttccgcg ttggaaatca atatcattac gttcaaactg
     8161 aatatcgacc aagtcattca agagtttatc acgagaaatc tctagaccag gacggagact
     8221 aacgacacta tcagcgtatt ccttgggcga acccaaacca tagatacaag agactgaggc
     8281 cacgacaata acatcattac gctccaaaag ggctgaggta gctgagtggc gaagcttgtc
     8341 aatctcgtca ttgacagaac tatccttctc aatataggta tcgctagaag ggacataggc
     8401 ctctggctgg taataatcat agtaggatac gaaatactca actgcatttt cagggaaaaa
     8461 ttccttaaac tccccataga gctgaccagc cagagtttta ttgtgggcaa taaccagagt
     8521 tggtttattg actttagaaa tgacctgact catagtatag gtcttccctg ttccagtcgc
     8581 ccccatcaga atctgagctt tttctccccc ctcaatgtta tccaccaact gctcgatagc
     8641 ttggggttga tctcctgatg gttgatattt tgatactagt ttaaattgat tatctgtaat
     8701 gtgattgatc ataagaatcc tctctccatg tgctattcct attttagcat acttatagta
     8761 ttttcacgaa gaaaaggacg gaccgaagtc acatcctttc attttctttc tttaatttat
     8821 tagcaagata gacaatcagc agtagtaata ccagacttgt cataatggaa ccttctatgc
     8881 caaaagaacc acctgatagc caatcttgat tgccttgtgg taaaaaggtc atcagagacg
     8941 ttcctgacgg ttgaccacta actaaaatcc caaagagatt accctgagca aaattccaag
     9001 caccatgaat acctgcaaca ccccaaactg tatcagtttt gagaagataa agagccatgg
     9061 caactccgaa taaaaagaga tttactagag atagaggggt gaggccagaa ttgcccatat
     9121 gaagcagggt aaagaacagg ctagatataa gaatagctag ttttagattg gttcttgagg
     9181 ccaattgagg aagtagccaa gcacgggcca ccacttcttc tgctgtcccc tgtaaaatcc
     9241 aaaatgggat agtaaagacg acaaaggcaa gagaataagg attcaagtga atggattcca
     9301 aacgatattg acctaagacc actaaaccta acaaggtcag aagaaaaagt gccaagccta
     9361 gactaaatcc tttcagaaga ttgctgagga aattctctct ataaaatccc aaggttcgaa
     9421 taggtctttt ctcaactttt cttgtccatc tgaacacagt gttaagaata aaaccaaagg
     9481 ccagtaatcc taaaattaaa cgaaagtcac ttgttttatc cgtcaagctc tgatgcaagg
     9541 tctgcaagta ggttgcaaga ttttgaccag cctctccaaa atttctagca agtccgatga
     9601 gagctaacaa gccaatacca tacaaagtaa aagccacaaa ctctcctatg aagacaaaaa
     9661 caaaggctaa caaggggctt gtgtaaatgt ttaaactcat ttttgaagct tggaagtctt
     9721 taaatattct tttgttcttc atatccccgt cctcttttct tctatatata ctattattat
     9781 agcacttttt aatagcaatt caagaaagaa aaggaaaaat caatgttata ttttctgaca
     9841 caaaaaatat aacaatttgc ctttttttac tttttctgat ataatgagag aatattcgga
     9901 aaaggagact aaaaatgaag aaaaaatttc tagcattttt gctaatttta ttcccaattt
     9961 tctcattagg tattgccaaa gcagaaacga ttaagattgt ttctgatacc gcctatgcac
    10021 cttttgagtt taaagattca gatcaaactt ataaaggaat tgatgttgac attattaaca
    10081 aagtcgctga gattaaaggc tggaacattc agatgtccta tcctggattt gacgcagcag
    10141 tcaatgcggt tcaagctggg caagccgacg ctatcatggc agggatgaca aagactaaag
    10201 aacgtgaaaa agtcttcacc atgtctgata cttactatga tacaaaagtt gtcattgcta
    10261 ctacaaagtc acacaaaatt agcaagtacg accaattaac tggcaaaacc gttggtgtta
    10321 aaaacggaac tgccgctcaa cgtttccttg aaacaatcaa agataaatat ggctttacta
    10381 ttaaaacatt tgacactggt gatttaatga acaacagctt gagtgctggt gccatcgatg
    10441 ccatgatgga tgacaaacct gttatcgaat atgccattaa ccaaggtcaa gacctccata
    10501 ttgaaatgga tggtgaagct gtaggaagtt ttgctttcgg tgtgaaaaaa ggaagtaaat
    10561 acgagcacct ggttactgaa tttaaccaag ccttgtctga aatgaaaaaa gatggtagtc
    10621 ttgataaaat tatcaagaaa tggactgctt catcatcttc agcagtgcca actacaacta
    10681 ctctcgcagg attaaaagct attcctgtta aggctaaata tatcattgcc agcgattctt
    10741 cttttgcccc ttttgttttc caaaattcaa gcaaccaata cactggtatt gatatggaat
    10801 tgattaaggc aatcgctaaa gaccaaggtt ttgaaattga aatcaccaac cctggttttg
    10861 atgctgctat cagtgctgtc caagctggtc aagccgatgg tatcatcgct ggtatgtctg
    10921 tcacagatgc tcgtaaggca acttttgact tctcagaatc atactacact gctaatacca
    10981 ttcttggtgt caaagaatca agtaatattg cttcttatga agatctaaaa ggaaagacag
    11041 tcggtgttaa aaacggaact gcttctcaaa ccttcctaac agaaaatcaa agcaaatacg
    11101 gctacaaaat caaaaccttt gctgatggtt cttcaatgta tgacagttta aacactggtg
    11161 ccattgatgc cgttatggat gatgaacctg ttctcaaata ttctatcagc caaggtcaaa
    11221 aattgaaaac tccaatctct ggaactccaa tcggtgaaac agcctttgcc gttaaaaaag
    11281 gagcaaatcc agaactgatt gaaatgttca acaacggact tgcaaacctt aaagcaaacg
    11341 gtgaattcca aaagattctt gacaaatacc tagctagcga atcttcaact gcttcaacaa
    11401 gtactgttga cgaaacaacg ctctggggct tgcttcaaaa caactacaaa caactcctta
    11461 gcggtcttgg tatcactctt gctctagctc ttatctcatt tgctattgcc attgtcatcg
    11521 gaattatctt cggtatgttt agcgttagcc catacaaatc tcttcgcgtc atctctgaga
    11581 ttttcgttga cgttattcgt ggtattccat tgatgattct tgcagccttc atcttctggg
    11641 gaattccaaa cttcatcgag tctatcacag gccaacaaag cccaattaac gactttgtag
    11701 ctggaaccat tgccctctca ctcaatgcgg ctgcttatat cgctgaaatc gttcgtggtg
    11761 gtattcaggc cgttccagtt ggccaaatgg aagccagccg aagcttgggt atctcttatg
    11821 gaaaaaccat gcgtaagatt atcttgccac aagcaactaa attgatgttg ccaaactttg
    11881 tcaaccaatt cgttatcgct cttaaagata caactatcgt atctgctatc ggtttggttg
    11941 aactcttcca aactggtaag attatcattg ctcgtaacta ccaaagtttc aagatgtatg
    12001 caatccttgc tatcttctat cttgtaatta tcacactttt gactagacta gcgaaacgct
    12061 tagaaaagag gattcgttaa tggcaaaatt aaaaattgat gtaaatgatt tacacaagca
    12121 ctatggaaaa aatgaagtcc taaaaggaat tacgactaag ttctatgaag gagatgttgt
    12181 ttgtatcatc ggtccttcag gttctggtaa gtcaactttc ctccgtagcc tcaatctttt
    12241 agaagaagtc actagcggtc acatcactgt gaacggctat gatttaactg aaaaaacaac
    12301 caatgttgac cacgtccgtg aaaatatcgg catggtattc caacacttca acctcttccc
    12361 tcatatgtct gtattggaca acatcacctt tgctcctatt gagcacaagt tgatgactaa
    12421 ggaagaagct gagaaattgg gaatggagtt gcttgaaaag gttggactag cagataaagc
    12481 taatgccaat ccagatagcc tatcaggtgg tcaaaaacaa cgtgtggcca tagctcgtgg
    12541 cctagcaatg aatccagaca tcatgctctt cgatgaacca acttctgccc ttgaccctga
    12601 gatggttgga gacgtactta acgttatgaa ggaattggct gagcaaggca tgaccatgat
    12661 tatcgtaacc catgagatgg gatttgctcg tcaggttgcc aaccgcgtta tctttactgc
    12721 agatggcgag ttccttgaag acggaacacc tgaccaaatc tttgataacc cacaacaccc
    12781 tcgtctgaaa gagttcttag ataaggtctt aaacgtctaa actcaaactg taaggatttc
    12841 cttgcagttt ttctacctcg tattggaatt tttgattttt cggaaaatta tgttagaatt
    12901 aagtttatga aatgaggttt cctcatacct agcaagacta ggaataaaaa tagaaattag
    12961 gtagctagat gtcatctaag gttattgtta caattttcgg tgcgagtgga gacctggcta
    13021 aacgcaagct ctacccttcc ctttttagac tatataaatc cggcaatctt tccaagcact
    13081 ttgccgttat tggaactgcc cgtagacctt ggagtaagga atattttgaa tctgtagttg
    13141 tcgagtccat ccttgatttg gcagatagta ccgagcaagc ccaagaattt gctagccact
    13201 tctactatca aagccatgat gtcaatgatt cggaacatta tattgctttg cgtcaattac
    13261 aagctgagct taatgaaaaa taccaagctg aacacaataa gctcttcttc ttgtctatgg
    13321 cacctcagtt ctttggaacc attgccaaac acctcaaatc tgaaaacatt gtcgatggca
    13381 aaggttttga gcgcttgatc gttgaaaaac catttggtac agattacgca actgcaaaca
    13441 agttgaatga cgaactccta gcaacatttg acgaagaaca aattttccgt atcgaccatt
    13501 atcttggtaa ggaaatgatc caaagcatct ttgcagttcg ctttgcaaac ttgatttttg
    13561 aaaacgtttg gaacaaggat tttatcgaca atgttcaaat tacctttgcg gagcgcttgg
    13621 gtgtagaaga acgtggtggc tactatgacc aatccggtgc cctccgtgac atggtccaaa
    13681 accacactct acaacttctt tcgctcctcg ccatggacaa accagcaagc ttcacaaaag
    13741 acgagattcg tgctgaaaag attaaggtct ttaaaaacct ctatcatcca actgatgaag
    13801 aactcaaaga acactttatc cgtgggcaat accgctctgg taagattgat ggcatgaaat
    13861 acatctctta tcgtagcgag ccaaatgtga atccagaatc aacaactgaa acctttacat
    13921 ctggtgcctt ctttgtagac agcgatcgat tccgtggtgt tcctttcttt ttccgtacag
    13981 gtaaacgact gactgaaaaa ggaactcatg tcaacatcgt ctttaaacaa atggattcta
    14041 tctttggaga accacttgct ccaaatattt tgaccatcta tattcaacca acagaaggct
    14101 tctctcttag cctaaatggg aagcaagtag gagaagaatt taacttggct cctaactcac
    14161 ttgattaccg tacagatgcg actgcaactg gtgcttctcc agaaccatac gaaaaattga
    14221 tttatgatgt cctaaataac aactcaacta actttagcca ctgggatgaa gtttgtgcgt
    14281 catggaagtt gattgaccgt attgaaaagc tctgggctga aaatggtgcc ccacttcatg
    14341 actataaagc tggaagcatg ggacctcaag ccagctttga cctacttgaa aaattcggtg
    14401 ccaaatggac ttggcaacca gatatcacct atcgtcaaga tggtcgctta gaataaaaaa
    14461 atttcctgca agtttatgcc ttgcaggatt tttgcttctg attagattaa accttccaag
    14521 agacctttca taaagttttc tgagttaaac tctccaatat catcgatttt ttcaccaaaa
    14581 ccaatcaatt ttacaggaat attgagttct tcacgaatgg ctagaaccac acctcctcga
    14641 gcagttccat caatcttagt caaaacaatt cccgttaaag gtgtgatttt cgaaaattct
    14701 ttggcctgta ctagggcatt ttgacctgtt gatgcatcaa gtgccaagaa ggtttcatgt
    14761 ggtgcttctg gcacaacacg tttgataata cgaccaatct tttccaactc agccataagg
    14821 ttatccttat tttgcagacg accagcagta tcaatcatga gaatatcgat accttcagcc
    14881 acggcacgtt ccataccatc aaagaccacg ctggctggat cagctttttc aggtccagtt
    14941 actactggaa catctactcg tcggccccat tcagctagct gagctactgc acccgcacgg
    15001 aaggtatctg ctgcaaccag catgaccttc ttaccagctt gtttgtagcg gtgggctagt
    15061 tttccgatag aagttgtttt cccaacacca ttcacaccaa caaagagcat aactgtcaag
    15121 ttatcttgga agtggatgct ttcatcgtag ctaccatcct tttcataaag ctcaaccaat
    15181 ttctcaatga tgacacgacg aagtgcatca ggtttcttgg cattttcaag cttggcttcg
    15241 taacgtagtt cctccgttaa gttagaagcg acttggacac caacatcact cataatcagc
    15301 agttcttcca gttcctcgaa aaattcttcg tcaacagagc ggaagttagc aaagaaggca
    15361 ttcaagcggg caccgaaacc tgtacgagtt ttcttaagac tgcggtcata tttttcctga
    15421 acggtttctt ctgcttgagg cctttctggt tcaagaactt cagaattatt ttcttctata
    15481 gtctctagaa actcagtatt cccttcttct gagtgtggaa attcttcgac ttctttttga
    15541 gaaacttcta caactggctc taaatccaga ctttcttcaa ctgtgtcttg gatttcctct
    15601 tcttggaaca cagcttgttc aacaatttca acctctgctt cttcctgaga aacttcctca
    15661 acttctgtga aggtaggatc aacatcttca gacaaatcaa gattttccag agcttctttt
    15721 acaacttctt cgattttagg ttcttctttt tttccgaata gacggtcaaa caatcccata
    15781 tcttagttct cctttagcac atattcttcg atagcccagg cgacagcttc ctcatcgttg
    15841 gtcatcggcg tcactacatt tgcggctgcc tttacttcag gaacagcgtt ttgcatagca
    15901 acaccaagac ctgcccattc aatcatagag aggtcattgg cctcgtcacc acaagccatc
    15961 acttgacttt ggtcgattcc aagatggctg attagttttg ccaaacctgt tgctttatga
    16021 acattctttg gtgaccattc tagcaacatt tcacgtgatt taaagatttc atattggtca
    16081 aacaattctg gagaaatctt ctgaatggct gcatccaagg gttcttgagc aaaggcagtc
    16141 acgcatttgt tgtaggtcat ttgactagat aagtcttcaa agtccactgg aacaaaggtc
    16201 aaagctggat tgaatttggc ataaagactt tcttggtccg attggatttg ataaactgtt
    16261 ccttctgaga tggcatcaag aggcagtgat aatttctctg tttcttcata caaacgtgcc
    16321 acatcatcat atgaaaagac tgttttatca aggatttctc ctgtattttt ctgaactaat
    16381 ccaccattaa aagtaatggt atactcatct tcctgaccgt cagtccctaa ctcatggaga
    16441 aagaaatcca tggcttttaa gggacgacca gttgtcaata cgaccttgat accacgatca
    16501 cgcgcagctt tcaaggtttc cttggtacga tccgtcagcc ttttatcagt agtcagcaag
    16561 gtcccgtcca agtccaatgc aatcaatttt atatctgcca ttataagccc tccatataag
    16621 ctataaccga ccgttcctta tggtgaccaa tcacagtctt tgctaattct aaaatttcag
    16681 gtcgtgcatt ttcaggagct acaggatgtc ccacaacctg catcatatgt aagtcattaa
    16741 gattgtctcc aaaagccatg acctgatcca ttgtgatacc aagtttttta gctaattcaa
    16801 caatagctac acccttatcg acatagtcca gaacaatatc aatagattca aagccagttg
    16861 tcatggcctt aacaccaggg acatgttcat taacccaagc ttcaccagct tctagcgttt
    16921 cttctgtgaa gttggttgta aatttgaaaa tgtcatctgt gatatcttcc aaactcgcta
    16981 ctttttggat attttcatta taatgctgac tcactttcaa ataggtctca tcaaccgtat
    17041 ctagaacata tgaacccttc ttacccgtca agagcagttt attgatatct acataaggtg
    17101 acgttttcag cttttcaaaa gttgccagat aaaagtcacg agacatagtc gcttcataca
    17161 agtcctgacc ttgatactct accaaactgc cattttccgc gatgaaaata atgtcatcac
    17221 gaacatcagc aaataatttt tccagagaca gaaatccccg acccgaagcc accgcaaagt
    17281 aaatcccttt ttccttgtag gaagccaaga gagacttgag acgatccata tcaaagcgtc
    17341 cattcccatc taggaaggtt ccgtccatat ccgttgctac tagtttaatt gtcatccttc
    17401 aatactttct aaatctttta acttaactga aacaatcttt gaaacacccg attcttgcat
    17461 ggtcactcca tagatggaat cagccgctgc catggttccc ttacggtggg ttacaacgat
    17521 aaactggctg tccttgtcaa agcggttgag gtaatcccca aaacgtttaa cattggcttc
    17581 atccagcgca gcttccacct catccaagat gacaaaagga atggtcttga cacgaataat
    17641 ggagaaaagc aaggcaagag ccgatagagc tttttcacca ccactcatga ggttaagcga
    17701 ctggattttc ttacctggag gttgaacaga aatctccaca ccagctgtta aaaggtcgcc
    17761 ctcagtcaat atcaagtctg cctgacctcc gccaaacatc tgcttgaagg tcactttaaa
    17821 ggactcacga atagcttcaa aggttgattt aaagcgttcc ttgacctcat cattcatctc
    17881 tgtaatggtt tcaaggagca gatttttcgc tgacaaaata tcatctcgct gactatttag
    17941 aaagtccaga cggttgtgaa cttcttcgta ctggtcaata gcttccaagt tgacaggtcc
    18001 caatgagcga atagccttct ctaaatcctg cacctcttgc tctgccagat tgaggttttc
    18061 caattcatgt gccttttcta gtgcttcagt atagctaatc tggtactggt ctgttaattg
    18121 attttgtaga tggcgcaagc gctcgctgac cttttctttc ttggcttcag cacgtgtttg
    18181 cttgcgaatc cactcctcat tctgctggcg agcttgatcc aagtgactgg caatatcatc
    18241 cagttgacct tctatatcat ccaactcaaa ctgcttgcga atcaaacctt gttggagatt
    18301 tgttttctga gttttggatt cttccgcctg ttgactgagc aattctgtat caaccttctc
    18361 aagattgtca actttttctt ggagcatgcg ctgaatttct tcttgttcga tattaagatt
    18421 gtccaattcc ttgcctaaac gctcaatatc agctacttca taacgttttt gcccttgcag
    18481 ttctgtctta agcaagcgag cttgcgccac ctcttcctgc aaattttgat agcgttcttg
    18541 gatggcgttt ttattagact taatttcttc aatctcagct tccagatttt gcttttcact
    18601 ggcgatagta gcaaggctct cttgacattt ttccttatcc gcttgccaat ctccctcaga
    18661 aagacgatct atttcctctt cttggagttt ccaaagagtt tccagttctt caacttgctg
    18721 actagtttgc tgataagcga gggacaagcc ttgctcctga atacgtgcct gctctccttg
    18781 agatttgatg gcttctaatc tttctgtcaa tgcagccatc tggtcttgca aggtcttcaa
    18841 agccgcttct tctgaaccca agcttgcttc atctgcagca atttcttttt gtaattgctc
    18901 cagttctggc ttgatgaaaa tactgttatt ttggcgattg gcaccacccg cataggaacc
    18961 acccgtgcgt aattctgtcc catccaatgt caccatacga acctgataac gaacttgtcg
    19021 agcagcttca cgcgcatgtt ctacggtatc aaagatagcc gtcgtagcta gcaagttctt
    19081 gaaaatggct tccagtctag tatcaaaagt caccaactca tctgccatcc caaggaaacc
    19141 tgggcttaca gcgatagcat cttggttctg actagaaatc gtacgcgcct taatagtggt
    19201 caaaggaaga aaggttgcac gaccgactct gtttcgtttg aggaaatcaa tagctttggt
    19261 tgccgactct tcatcttcta cgatgatatg ctggctactt gcccctaagg caatctctag
    19321 ggcagtttga taataaacat caaaggtcag atgctcactg actgcaccaa taatcccacc
    19381 taggcgatct ttttcttgga gaacactctt aacacctgca taaaagttac tatgatttct
    19441 caggatattt tccaaacttt gagctctggc ctgcttgttt ttgagactat ccagacggtc
    19501 aaagagttga ctttgttgag cttgatagga agttttctgc tcctcttgct ccttggcaat
    19561 agcttgatag tcagccaata atttctgaac ctgcaccttg gcagtttcaa gctcgtcttt
    19621 ttgctgacta gccttctctt tagctgtagc taattgctct ttcagctttt ctagttgatc
    19681 tgcttgtttt tgagaaagct gacgactatt ttccaactca ttctcaatac gggtcaactg
    19741 gtttgagaca tccgcttctt cttgtaaaag agctacaaag cgttcacgta agagctcaat
    19801 catctgatca ggatcgtctg agaaagccag caattcagct tctaaacgat tgagtttttg
    19861 attattttgg actagatttc cctctaacag agctaaagag ctttctttat catacttttc
    19921 tttgctgagt gaatttctct tatcctccaa agcagccaaa cgagcttgtg cctcctgttg
    19981 attcagggcc acttgctcgg actccagttt cgatagggct aattttcttt ctaaatcact
    20041 aatcagacta gtcaagtcca tcaaactgcc ttggtctttg gccatttcag cctgtaaatc
    20101 ttggcgttgc tttttaagag tttgattttc ttcttctaat ttttcacgct tttggtaata
    20161 actcatcaag agttcttgaa cctgagccaa ctcttcttct gtcgactcta gttctgcctt
    20221 attttccttg atttgagcaa ccagaacgtc taaataaata gccttacgtt gtccttccaa
    20281 gtctaaaaac ttacgggcat tctcagcttg cttctcaaga ggcttgattt gattatccaa
    20341 ctcgtagata atgtcctcta agcggtccag attatcctga gtttgctgca gtttactctc
    20401 ggtttctttt ctgcgagtct tgtattttaa aactccagca gcttcttcaa aaatagctcg
    20461 tcgttcctca ggcttggaat taaaaatctc ctcaaccttc ccttgggaaa taatagagaa
    20521 ggaatctcgt cccaatccag tatccaagaa gaggtcatga atatcacgca gacggacttt
    20581 cttgccgtca atcttgtatt cgctatctcc actacgatag atatggcgtt ccaccctgat
    20641 ttcttgacct gcatccttga taaatccgtc atgattatcc agagtcacaa ctacagaagc
    20701 ataattgagc ggtttgcgac tttctgttcc agcaaagatg acatccggca tcttgccccc
    20761 acggagactc ttgacactag actcccccaa agcccaacgc agactttctg taatattgga
    20821 ctttccagat ccattgggtc caacaactgc cgtcacacct tggtcaaaaa cgaccttggt
    20881 cttatcagca aaagacttga acccctgaat ttcgatttcc tttaaataca tgaatccagc
    20941 cctttctcaa cggcattttt ggcagcttcc tgctctgcta atttcttaga acgaccttgg
    21001 ccttgaccga tgctcttacc ttcaacaaga acttctacat caaagacctt atcgtgagca
    21061 ggccctgttt cagaaatcac ctgataacga atagccacat caccattgac ctgaagcaac
    21121 tcttggagat gggttttata gtctgtaatc atctcaaatt cgcctgcttc taccttagga
    21181 atcataactt ggtagataaa ttccttgacc tttgccacat ccttatccaa aagaagggca
    21241 ccaagaaagg cttcaaaggc atcaccaaga atggtgtcac gattgcgacc accagatttt
    21301 tcttcccctt tacccaactt gataaactgg tcaaactggc aatcacgcgc aaaaccagct
    21361 aaactctcct cacggacaat catagcacgg agttttgata ggtcaccttc aggcttttta
    21421 ggatattttt tatatagata ttctgaaatc aataactgta gaacagcgtc tcctaaaaat
    21481 tccaagcgtt cattgtgtga aatttttaag aggcggtgct cattggcata actcgtatga
    21541 gtaaaggcag tttccagtaa ctttttgtct gcaaattcga tttcaaaatg attctttagt
    21601 acagtttgta attctttcat accaacttct ttctaactga taatagtcct ttttattata
    21661 tcaaaaaaag ccccctgagt cactctaaaa cgggactgga aagcatttgg gaattcttta
    21721 gacagagatt ctcagtttta gcggcaaatt tgggtcagga taaagaaaaa agccctatta
    21781 aaggcttttt aggatgttta catccaccct gagggaatcg aacccccatc tcaagaaccg
    21841 gaatcttacg tgatatccat tacactaagg gtggaaactt gttttattat aacagaaatt
    21901 tgctctaata acaagttttt tggtcaaaga ccccgtctta gtgggaagca tccccattcc
    21961 agatggagtt tttcacgatc acataatcaa cgtgtttaag gtcagcaacc tgacgtccac
    22021 ctgcataaga aatagcactt tgaaggtctt gttccatctc agttaaagtg tcttgcagat
    22081 gacctttagc aggaagcaag atacgtttgc cttccacatt tttgtaagca cctttttgat
    22141 attgtgaggc tgaaccataa tattctttga actgttcacc atcgacttca atcgttttcc
    22201 ctggactttc aatgtgtcct gcaaagaggg aaccaatcat gatcatgcta gcaccgaagc
    22261 ggatagactt ggcaatatca ccgtgagtac gaattcctcc atcagcgata atcggtttac
    22321 gcgcagcctt ggcacaccag cgtagagcag ccaactgcca accacctgta ccaaaaccag
    22381 tcttaacctt ggtgatacaa accttaccag gaccgattcc gaccttagta gcatccgcac
    22441 cagcattttc caattcacgc acagcttctg gtgttcccac atttccagca atgacaaagg
    22501 tatctggcaa ttctttcttg atgtgttgaa tcatagaaat cacgctatcc gcatgaccat
    22561 gagcaatatc aatagtgata tactcaggag catcagcctt gagctggcta acaaaatcat
    22621 actcatagtc cttaacaccg acagagatag aagcaatgag tccttgatcg tgcatgcgtt
    22681 taataaaagg aatgcgtcct gcctcatcaa aacggtgcat aatgtagaag taaccacctt
    22741 tagccagttg ctctgctaca ttttcatcca aaatcgtctg catattcgct ggcacaacag
    22801 gtagtttaaa ggtgtgattt cctaaagtga cacttgtatc cgcttctgca cggcttttaa
    22861 tgacacattt atttggaatc aattgaatat cttcgtaatc aaaaattgga aattcattta
    22921 acatatcgat gtctcgtttc ttttgtaatg acctacctat gctctcgcat cactacgcct
    22981 tttccttaat ttattataaa ctaaagtaca gtttttgtca aattatttta tgaattaaaa
    23041 tatattgttc gggttttgct tgtgttaaaa ataaaagaaa gaggggaaat tattttctcc
    23101 ccacttcttc atcaataatc gctttaaaat tttcttctga attttccatc attttacaaa
    23161 atgcactata gaatgaggca ttctgaggga ttcgaataga ccacttcttc aattgagctc
    23221 cataacctgc taggtttccc atctcattat caataacctt gtccttgctc ggaaaaacaa
    23281 gtccagcctt ctcagctttt aaaatataag aataggaaat cagctggaat aagtcctcac
    23341 ggttgattcc tttttcagtc aattccagtt ttttatattt tgcatctaga acaatctttc
    23401 gttctctgtc ataaaaatct ggatatactt ttcgtttccc aacagaaaat actgaaattc
    23461 catccgtctt atccttattt ctgggatgta caaaaccttt tggcaacaag gtgtaaacat
    23521 actcttccca aagccaggca acatcaaaga gaataccata gattttttga tcttgatacc
    23581 ctaaaccgtg cttttcttgg tttaggatca tcagacaaag ttcttgtaag tttctgtact
    23641 cgtgaaagta tgcatgacgt ataggtttag attgatttct ccgagtaatc ttagcacgat
    23701 cagctagttt ataagagggc gttacacgca cgatttcaga tacgttttca cgactagttg
    23761 agagattatc tagtacccct tgaccaatgc ttttctgatt cttaatgtat tcaatagtgt
    23821 gacggaccaa ctgcatgagg ggattatcat aggtgaactc tctcgttgtg taggcaatat
    23881 ttcccgtgaa aggaagattt ttcttgagat ggtttcttac atcaatcact cccttaacat
    23941 gactgtcgtt atgagaaaat ctctggtatt ccttataaag accttttcga atagcagctt
    24001 gtagatactt ggggaagaga tacatcaaaa gttgataaag cctctcttca cgagacaaag
    24061 caacatctaa actagtgaga ttgatatgaa gaaccttgtt taagagataa tgcaaaaaat
    24121 ggtcattact ctcatcagaa aatcgtgagg aaatcgttaa tctttcctga ccatatccaa
    24181 gaaaaccaat cacgttccct gtcttgattt tctgattgac tgtttcaaaa atcttttggt
    24241 ccttatccaa atcaggagta tgagtcaaat catttggaaa aatgaaaata cgttcatctt
    24301 gggatagact ttccaaggtt ctgtctaaaa gaagattgct tagtttagga tattcttcaa
    24361 caaatttttc tttaataatc ttgtgttgat tatcagtaat ccgcatcatc gttttcatcg
    24421 ccttcattgt catcagctac tgcctgatct ttttgctcat tatttgtcag ttcaaatgct
    24481 tttttcaaag tttccaaagt ttcaacctca tcataagaac ctcgcaagta gtcttctagg
    24541 agaggcttaa tataatcaga ccagagtaat tcatagtcaa aatctacatc cttcaactta
    24601 agaaaataac ttggtccaat atgataatga ctgtttaatt cctgaacatt ttcgatagca
    24661 gcgttcaagt ttcttagacg aatttttgct tcttctgcat ggatattcag ctctttatcc
    24721 aacatgccaa cttgaccctc ggcagtaact tcaacaaaac gaaaacgacg acgcatagca
    24781 aaatcaaagg tatccactga acgatcaata tcattcatag ttccgatgat gtaaacattt
    24841 tcggggatat agaacttttc atcagtttcg tgtagatttg catattgggt agaaacactt
    24901 cctttttcac cacgatagtc agggtcgata gagaaaaaga gttcgccaaa aatcttagaa
    24961 atctccccac gattgatttc atcgatgatg aagacgaatt tcttatcagt atcaatttct
    25021 gttggggaaa catagtcttt caaaccaaat ctctttctca atgtttctag gacagttttt
    25081 ccaccacttt tgtagtattc ttgcttatta taatttttat ctttatacaa ctcgtaaaca
    25141 tatttgctag gtagtgacca tcctggaaca ccactatcat aatttactga
//
LOCUS       DAVKGC010000017        24916 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKGC010000017 DAVKGC010000000
VERSION     DAVKGC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24916
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.017"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(74..271)
                     /gene="blpI"
                     /locus_tag="VW049_000572"
     CDS             complement(74..271)
                     /gene="blpI"
                     /locus_tag="VW049_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEU9431740.1"
                     /translation="MNTKMMEQFEIMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            552..1139
                     /locus_tag="VW049_000573"
     CDS             552..1139
                     /locus_tag="VW049_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9431741.1"
                     /translation="MTSYKRTFVPQIDARDCSVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLAPKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLISHYYQYRWFLLSPRNLR"
     gene            1066..2709
                     /locus_tag="VW049_000574"
     CDS             1066..2709
                     /locus_tag="VW049_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9431742.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLLSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2720..4081
                     /locus_tag="VW049_000575"
     CDS             2720..4081
                     /locus_tag="VW049_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9431743.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            4138..4266
                     /gene="blpC"
                     /locus_tag="VW049_000576"
     CDS             4138..4266
                     /gene="blpC"
                     /locus_tag="VW049_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9431744.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(4337..5677)
                     /locus_tag="VW049_000577"
     CDS             complement(4337..5677)
                     /locus_tag="VW049_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9431745.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLLKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5691..6428)
                     /locus_tag="VW049_000578"
     CDS             complement(5691..6428)
                     /locus_tag="VW049_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9431746.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALNYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(6442..6771)
                     /locus_tag="VW049_000579"
     CDS             complement(6442..6771)
                     /locus_tag="VW049_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9431747.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLK"
     gene            7048..7377
                     /locus_tag="VW049_000580"
     CDS             7048..7377
                     /locus_tag="VW049_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9431748.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7545..8594)
                     /locus_tag="VW049_000581"
     CDS             complement(7545..8594)
                     /locus_tag="VW049_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9431749.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWIFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFCQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8591..9322)
                     /locus_tag="VW049_000582"
     CDS             complement(8591..9322)
                     /locus_tag="VW049_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431750.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTMQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLDQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9390..9800
                     /locus_tag="VW049_000583"
     CDS             9390..9800
                     /locus_tag="VW049_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9431751.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9810..10097
                     /locus_tag="VW049_000584"
     CDS             9810..10097
                     /locus_tag="VW049_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431752.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            10279..10527
                     /locus_tag="VW049_000585"
     CDS             10279..10527
                     /locus_tag="VW049_000585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431753.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDRTDEVSNHTHGKPTLTWFEEIFKEYYSPSKASLI"
     gene            complement(10497..11633)
                     /gene="dnaJ"
                     /locus_tag="VW049_000586"
     CDS             complement(10497..11633)
                     /gene="dnaJ"
                     /locus_tag="VW049_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9431754.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGDGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGQGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12035..12391)
                     /locus_tag="VW049_000587"
     CDS             complement(12035..12391)
                     /locus_tag="VW049_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU9431755.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12393..14216)
                     /gene="dnaK"
                     /locus_tag="VW049_000588"
     CDS             complement(12393..14216)
                     /gene="dnaK"
                     /locus_tag="VW049_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9431756.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14696..15220)
                     /gene="grpE"
                     /locus_tag="VW049_000589"
     CDS             complement(14696..15220)
                     /gene="grpE"
                     /locus_tag="VW049_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9431757.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15247..16281)
                     /gene="hrcA"
                     /locus_tag="VW049_000590"
     CDS             complement(15247..16281)
                     /gene="hrcA"
                     /locus_tag="VW049_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9431758.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKTLQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKRIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16445..16954)
                     /locus_tag="VW049_000591"
     CDS             complement(16445..16954)
                     /locus_tag="VW049_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9431759.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            17563..19896
                     /locus_tag="VW049_000592"
     CDS             17563..19896
                     /locus_tag="VW049_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9431760.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKEDSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            19909..21372
                     /locus_tag="VW049_000593"
     CDS             19909..21372
                     /locus_tag="VW049_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9431761.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            21372..22940
                     /locus_tag="VW049_000594"
     CDS             21372..22940
                     /locus_tag="VW049_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9431762.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLAR
                     FIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYVWAVADSHVTVIRV
                     LSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIV
                     DKIEQFFAHIDALI"
     gene            22997..23794
                     /gene="psrA"
                     /locus_tag="VW049_000595"
     CDS             22997..23794
                     /gene="psrA"
                     /locus_tag="VW049_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9431763.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            23805..24404
                     /locus_tag="VW049_000596"
     CDS             23805..24404
                     /locus_tag="VW049_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9431764.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVATSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQL"
     gene            complement(24358..>24916)
                     /locus_tag="VW049_000597"
     CDS             complement(24358..>24916)
                     /locus_tag="VW049_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9431765.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIIFKVDLLFQKVSQLFE"
BASE COUNT         8019 a         4873 c         4542 g         7482 t
ORIGIN      
        1 tgatattttg taagtaagta aataagacag taaattaata gcgataataa tactatattt
       61 aagaatcata atcttacaaa gaggacataa ttcctgaacc tacacaaata agtgttgctg
      121 ctcccccagt tatcggacca gtcgcagcag ctaatagtac tgctccaata caaccaccga
      181 ttgcagatcc taaattgcct cttcctccac taactatttc gagttcttca ttatccataa
      241 tctcaaattg ttccatcatt tttgtattca tgacaaatac tcctttttct ttttttattt
      301 ttgtcttgtt gtaactttga taagtttagt atatcatcgt tttttaaaat ttttcatcca
      361 gatcttgaat tgtcatcgaa acgtcttgaa ttagcttttt tatttcaagc cacctctaaa
      421 tgtttaaaaa aaataatttc taatcacttt tttaccattc aggaagtttt aatgactatt
      481 caagatttca taaaatatga acttagtttt atgacataat agacctatcc actatatgaa
      541 aagaattgcc aatgacttct tataaacgta catttgttcc tcaaatagat gcgagagact
      601 gtagtgtcgc tgccttagcc tcgattgcta aattctatgg ttcagatttt tctctagctc
      661 acttgagaga acttgcaaag accaataaag aagggacgac tgctcttggc attgtaaaag
      721 ccgctgatga aatgggcttt gaaacaagac ctgttcaagc agataaaacg ctctttgaca
      781 tgagtgatgt cccctatcca tttatcgttc acgttaacaa agaaggaaaa ctccaacatt
      841 actatgttgt ctatcaaaca aagaaagact atctgattat tggtgatcct gacccttctg
      901 taaaaatcac taaaatgtca aaagaacgct ttttctatga atggactgga gtagctattt
      961 ttctagctcc caaacccagc tatcaacccc ataaagataa aaagaatggt ctactaagca
     1021 agcttccttc ctctgatttt caaacaaaaa tctctcattg cttacattgt tctctcaagc
     1081 ttattagtca ctattatcaa tataggtggt tcttactatc tccaaggaat cttagatgaa
     1141 tacatcccaa atcagatgaa atcaacttta ggaatcatct cagttggtct ggttatcacc
     1201 tatatcctcc aacaagtcat gagcttctcc agagattatc tcctaaccgt tctgagccag
     1261 agattaagca ttgatgtgat tttatcctat attcgccata tttttgaact tcccatgtct
     1321 ttctttgcga cacgtcgtac aggagaaatc atttcacggt tcacagatgc taactctatt
     1381 atagatgcct tggcttctac cattctttct cttttcctgg atgtttctat tctgattctt
     1441 gtagggggcg tcttactggc acaaaaccct aatctcttcc ttctttctct tatttctatt
     1501 cctatataca tgttcatcat cttttctttt atgaagcctt tcgaaaaaat gaaccatgat
     1561 gtcatgcaaa gtaattctat ggttagctct gccattatcg aagatatcaa cgggattgaa
     1621 actataaagt cgctcacaag tgaagaaaat cgctatcaaa atatagacag cgaatttgta
     1681 gattatttgg aaaaatcctt taagctcagt aaatattcta ttttacaaac aagtttaaag
     1741 cagggaacaa aattagttct gaatatcctt atcctatggt ttggcgctca attagtcatg
     1801 tcgagtaaaa tttctatcgg tcagctgatt acctttaaca cacttctttc ttactttaca
     1861 actcctatgg aaaatattat caacctccaa accaaactcc aatctgcgaa ggttgctaat
     1921 aaccgtttga acgaagtcta tctagtcgaa tctgaatttc aagttcaaga aaaccctgtt
     1981 cattcacatt ttttgatggg cgatattgaa tttgatgacc tttcttataa gtatggtttt
     2041 ggacgagata ccttaacaga tattaatctc acgattaaac aaggagataa ggttagccta
     2101 gttggagtta gtggttctgg taaaacaact ttagccaaaa tgattgtcaa tttctttgaa
     2161 ccctacaaag gacatatttc catcaatcat caggatatta aaaacattga taaaaaagtc
     2221 ttgcgccgtc atattaatta cctaccacaa caagcctata tctttaatgg ctctatcttg
     2281 gaaaacttaa ctttgggcgg taatcatatg attagccaag aagatattct aagagcttgt
     2341 gaattagctg aaattcgtca agacattgaa agaatgccta tgggctatca aactcagctc
     2401 tctgatggag ctggtctatc aggaggacaa aagcaacgaa tagccctcgc tcgtgctctt
     2461 ttaactaaat ctcctgtttt aatactagac gaagctacta gcggtcttga tgtcttgact
     2521 gagaaaaagg ttatagataa tcttatgtct ctaactgata aaaccattct ctttgtagcc
     2581 catcgtctca gtatagccga acgaaccaac cgtgtcattg ttcttgacca ggggaaaatc
     2641 attgaagttg gtagtcacca agagttaatg caggcgcaag gtttctacca tcatctattc
     2701 aataaataag gagaatgtca tgaatcctaa tctttttaga agcgtcgagt tttatcagag
     2761 acgttaccat aactatgcga cagtgttaat tatacctctt tcattactat ttactttcat
     2821 cttgattttc tcccttgttg ccacaaaaga aattactgtt acttcccaag gagaaatcgc
     2881 ccctacaagt gtcattgcct ccattcagtc aaccagtgat aatcctatcc tagctaatca
     2941 tttagtggca aatcaagtag ttgaaaaagg ggacttactc atcaaatact ctgaaacaat
     3001 ggaagaaagt cagaaaactg ccttagcaac tcaattacaa agacttgaga agcaaaaaga
     3061 aggacttgga attttgaaac aaagcttaga aaaagcgact gatctttttt ctggcgagga
     3121 tgaatttggc taccataata cctttatgaa ttttactaaa caatcccatg atattgaact
     3181 gggtatcaca aagactaaca ccgaagtttc aaatcaagct aatctttcca atagcagttc
     3241 atcagctatt gaacaagaaa ttacaaaagt tcaacaacaa attggagaat atcaagagtt
     3301 gagagatgct atcataaata acagagcacg cttaccaact ggcaatccgc accagtcaat
     3361 tttgaatcgt tatcttgtag cctcacaagg acaaacacaa ggaacggcag aggagccatt
     3421 tttatctcaa attaatcaaa gtattgcagg tctggaatca tctatcgcaa gcctcaaaat
     3481 tcagcaagct ggtatcggaa gtgtagcaac ttatgataac agtttagcaa ccaaaattga
     3541 agtactccgc actcagtttt tacagacagc ctcacagcaa caactaactg tggagaatca
     3601 attaacagaa ttaaaagtac aactagatca agccacacag cgtttggaaa acaatacctt
     3661 aacctcccca agtaaaggta tcgttcatct gaacagcgaa tttgaaggta aaaatagaat
     3721 tccaactggt acagaaattg ctcaaatatt ccctgtcatc acagatacaa gagaagtact
     3781 aatcacttac tacgtatctt ctgactatct acctctacta gataaaggac aaactgtaag
     3841 attaaaactg gagaagattg gaaatcacgg caccaccatc atcggccaac ttcagacaat
     3901 tgatcaaact cctaccagaa cagagcaagg aaatctcttt aaattaaccg ctcttgcaaa
     3961 actatctaac gaggatagta aactcatcca atatggctta caaggtcgcg tcactagtgt
     4021 aactacaaag aaaacatatt ttgattattt caaagataaa attttaacac attctgatta
     4081 attttcagat aacactctat aactatttat tatcttatca aaaaggagaa tcataacatg
     4141 gataagaaac aaaacctaac ttcatttcaa gaactaacaa ctaccgaact caaccaaatt
     4201 acaggtggag gattgtggga agatttatta tataacatta atagatatgc tcattacatc
     4261 acataagaac ttcatcatcc aatacaacta taaaaaaata agaccgagaa acaagtactc
     4321 tcggtcttat ttttcatcat tctgtatgta tcacagtaag tacctgacga aagacttgat
     4381 tttgacaggt ggtatttaga ctggtattag gatggctttc cacaatcttc atgacggtat
     4441 agagaccaac tcctctctcc tcccctttag aactggctcc aaaggagaag atttcagaaa
     4501 tatcgatgcc ctcttctttg atggagtttt cgatgataaa ggtctcctgt gctccatttt
     4561 ttaaaaaggc gattgaaaca tgaggttgac tagcttccac actggcttca atagcattgt
     4621 cacaaaggat agacacaatg gttagaaaat caagtagact catcccctcg acctgaatct
     4681 cctcaggaac ttcgacatta aagacaatgt tcttatctct ggcttttaaa aatttccctg
     4741 ctagaagact tttgagggct ttatcacgaa tatttaccaa tctgcccagg tcatatttat
     4801 tgttctgcaa tttctgactg gaatccttta agacggagcc atagacctct tttatctgct
     4861 ccatatcctc ctcttcaatg cccagacgta agctagtcaa gaggttggta taatcatgac
     4921 gaaagctccg tacttccttg taaagctcct ctatatgccg actatagcgt tccatatctc
     4981 tatagcgcag ggcctgctct tgttccaatc tctcatagag tttttccttc aaataggtat
     5041 ccaatttctt gataaccccc ataaaaaaga gtaggtaaaa gactaggatg agatggcgaa
     5101 cagtctttga ttgaatactt tgttcatatt caaaaaaaga cagactttcc atgactaaat
     5161 agtagccacc cattatccag ttaatctgag tcagggactt ttgaaaggct ttatcgagaa
     5221 tctcctttct caagctagta aaatcgtagt ccaaccattt caaaaaagct agagaaatga
     5281 agaaattgaa aattattata cataacccag taaatgagta gccatcatat acttgccctt
     5341 gtcccaaaaa tggaagcaca aaataggaga ctcctctata aaagagattc accaatatca
     5401 ttggaaagag accataaaag aaaaggagtt ttttaggaag ccctctcaat aataagaaag
     5461 ataagcctat gccgtacaag ggttccataa aataagatag gtaaacattt cctactatat
     5521 agctaatcat cacaaaaaca aaggccaaca gtatcttcaa aagaaaggcc ttaaaaatcc
     5581 tctcgaaagt aagatcaatt ccatccacct taaagaagat gacaatttct agtccattag
     5641 taacaagtgt atacaacaat atccaagcaa tgttcataaa ttctcctagc tcagtgtaat
     5701 ttattgatgg cctcagacac ttccctgacc ttataacggg cgattagaca acttccacca
     5761 ttgggaaaga aaagcagttt ttctttctta tccaaatgca ccacatttgc aggattgatg
     5821 agaaaagagc ggtggcactg caagagacgg ggctcctgct tgaaaacctc ctctaaactc
     5881 gctgtaaatt ccagcctgtc tgtcttggta tagagaataa cacgatgggc tctgggcgac
     5941 gtttcgagat agtaaacctc tttaaaagga tactgaaatt gggcaaattt tgatttaaag
     6001 taaaagcaat cttccgccag acttttacta tcttgactat tggcatagag gagggctgtc
     6061 tcgatccgag attcaaactc ctctgctgac aaggccttat caatgtagtt caaagcagac
     6121 acttggtagc gaaaagacag gggcataaac tccgagtgag tcgtcacaaa gacaatcagg
     6181 gcataaggat cccgatcccg aatctttcta gccacttcca gtcccttcat ctcttcattt
     6241 cgaatctcaa tatccaaaaa gaatagctga tgggccccct tctcatgcac ttcagccagc
     6301 agttggtccg gcttgccaaa tacctcaaaa gagctaggaa tgatatgatg tgctttcaaa
     6361 agtttctcaa tcgtcgtttc aattctagtc tgttgggaaa aatcatcctc taaaacaaat
     6421 attctcatct ttttactctc cttattttaa ccatttttga cgaatttctc tatagcgacg
     6481 tctggcatac ttgacagcgt attgaccttc ttctccgaga aaaaggattc tttcttgaaa
     6541 atcaatcgat tttaatttat caagattaac caaacaattt cgatgacatc tcatcaaggt
     6601 ttccccacat tggttctcaa gattacttaa attttgaagc atattaaaac tagcttcttc
     6661 tgtaacaatc tgtacggtat gggctttagt tggatgtgtt tggatatagt agatatcgtc
     6721 gacgtttact ttatagactg ttttctgcgt ctgaataatc atatgtttca ttggattctc
     6781 ctcctaagaa aagaataaca gaaaaggtct agcccaagcc cacccaatcc taaacagtct
     6841 ttgaaagctc ctaaaaggat tatttttcta caacatcatg atgagcttac aactatttta
     6901 tcataaaatt cactcagcgc aattcaagac atttcaatga caattaaaga tttgaataaa
     6961 aaaaaagaat taaaaatgat aaactaacag tatcaaaaca cagttgccag aagtaagact
     7021 ggcacccaga ttaaaggagg cattcccatg acagatacag accccatcaa aagagctcat
     7081 actttgatta ctgacttaaa caaagcctac caagcatgca aacaggcaag cgctgacgac
     7141 gtccgctttc aggagcaatt aaactctatt cttggttttc tagccaaggc tgaaacagtg
     7201 gataatcgat tcttgattga actagaaaaa ttttaccaga cttccagtct tctcatggga
     7261 ctcagcgctc ttgatccaga tgctccaact cgcgccgctt ggcgggccta tgaccgcttc
     7321 cactttgacc aagtcaagac caagttaata ctcaatgaaa atcaaagagc aaactaggaa
     7381 gctagccgca agctgtactt gagtacggta aggcgacgct gacgtggttt gaagagattt
     7441 tcgaagagta taagtctcta cggaccaacc attatcctgt agtaacaaaa aagaagctga
     7501 gacagactga actcaacttc ttttttagtg tcgtatcagc aatgttagtc ctgcatctga
     7561 cgttttacct gataaggcaa atacaagact agtaaaacca aaccagctcc tagtaaggct
     7621 agaagggcca gtttttcttg gaaggtaatc aacccaacaa ctaattccac aagtaaaaca
     7681 aaactggtca atcctcgaac tacctcctgt aagccttttt ccttttgccc cttgtccagc
     7741 ggaaagagtt gggtcaaata ctggtagtca aaggcatgat agagggccag caactggaag
     7801 agcaagaggt agttaaagag aactaccact gctgtcgcaa tccaagcttg ctcgataaaa
     7861 acctgcgcca gtacggaaag taagagaaga cgaagactga gagcaaagag gtcgccattt
     7921 cgcagataag aacgcagata gagattttgc caaatcttcc caggcacctt ctgaacagcc
     7981 tttaaaataa agtccagata ggcacgacgc ttaacgctgt ttgaaattcc cttgacctgc
     8041 gtaaagaggg caaagaaacg aagcaagact tgcttacgct tgctttcttg agaaataaga
     8101 tagtcccagt ccagtccagt ttcagtgaaa aatttgctgg ccttttgaca aaagtggaaa
     8161 tattttccta cccccaataa aagcacatag agcagaaaaa ctggcaagcc ataacccatt
     8221 gctaaaaata aaggcgcaaa taaaagcaag aagagggtct ggacaaaaat ccaaaagact
     8281 agggaaatgc cagtttgacg cttgagatgg agcttaattt cctcttctcc aactaagaga
     8341 aagagcttgt ctggagcctc catataggtg gcaattcctc cccaaagtaa aagtaaaaca
     8401 gacgtaattc ctacaaacaa aaggataggc caatgatttt caggaaaatg ttgtaagagt
     8461 tgactgtact ggtaggctag aaagcccaac aggacaagca ggaacaagac aaagtggtca
     8521 ttgagcacat agcgcagata accaagacac tccttacgaa aggcctgctt tctctttaaa
     8581 aacaagtctt tcataggtcc tcctcttttg tcagagccaa gtaaatatca ttcaaactag
     8641 cctcaggcat atcaaaggct tcgcgtagtt gcaggagatt gcctttggca cgcacctctc
     8701 ccttgtgaag aatgacaaag gcatcacaca tcttctccgc cgaatccagc acgtgggtac
     8761 tcatgagaat agacttgccc ttttgcttct ccacttccaa aagctgaatc aaatcagaaa
     8821 tagctagcgg atcaagacca aggaaaggct catccacgat gaaaagactt ggatccacca
     8881 caaaagcaca gataatcatg accttctgct tcatcccttt tgaaaaatga acagggaacc
     8941 agtctaattt ctggtccaga cggaacatct ttaacaaggg ctctactcgt tcgaaagcca
     9001 ctttttgctc aataccgtaa gccatagcaa ccgtttcgat atgctctctg agggtcaatt
     9061 cctcatacag actaggcgtc tcaggaatgt agccaatctg cttgcggtag ctagtcgcat
     9121 ctccttgcat agtcaggcca ttgatattga tggagccact ataaggtgcc aacagaccga
     9181 taatctcatt gatcgtcgtt gatttcccag caccattgag accaatcaaa ccgaccaact
     9241 gcccactttc aacagtaaag gacacatctt tcaaaacagg aacatgaaca tagccacctg
     9301 tcaggttttt aatttctaac atattttctc caaatctggt ataatgtagc tatattatat
     9361 caaaattcag tacagtagag gtagatttta tgtcagattg cattttttgt aaaatcatcg
     9421 caggggaaat tcctgcttca aaagtatatg aagatgagca ggtccttgcc tttcttgata
     9481 tctctcaagt aacactagga cacaccttgg tcgtgccaaa agaacactat cgcaatcttt
     9541 tggagatgga tgctacgagc gccagccaac tctttgccca agtaccaaaa gtagctcaaa
     9601 aagtcatgaa agtcactaag gctgctggta tgaatatcat ttccaactgt gaagaagtcg
     9661 ctggtcaaac agtttttcat actcacgttc accttgtgcc tcgctacagt gctgacgatg
     9721 acctcaagat tgattttatc gcccacgaac cagactttga caaacttgct caagtcgctg
     9781 aaaccatcaa aaacgcttaa ggagttgcta tgaaattatc caacctactg ctatttgcag
     9841 gagctgcagc cggaagttat ctggttacaa aaaatcgcca aaccatcaca gatgaagtct
     9901 tgaataccac tgaccgcgtt caagctatca aggacgatgt ggatattatc caaaacagcc
     9961 tgcaaatcat taaccagcaa aaagaactta tcaaggaata ccaagaagac ttgacttaca
    10021 agtttaaggt cttggaaaag gatatccaga ctagactagc tgtgataaaa gaaatgcagg
    10081 gaactgaaga taagtaaaaa gagccccccg gctctttttt aaaacgtaaa caagttatag
    10141 tgaattgaat ttagaatagc acaccatagt ttctaaagca ttgttagaga ttactttaaa
    10201 atcctcttat caatttgttc atattctatt caatttgttc atattctatt tcaatctatt
    10261 atatatatcc catcaactat gcatcataat ttaggtaact catactcaat aaaaatcaaa
    10321 aagcaaacta gaaagctagg cacagactgc tcaaaacacc gttttaaggt tgtggataga
    10381 actgacgaag tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagt
    10441 aaccataccc atggcaagcc gacgctgacg tggtttgaag agattttcaa agagtattat
    10501 tctccatcaa aggcatcttt aatatgatca aagaagcctt ttttctttgg atttactttc
    10561 aagtcaccag cagccgcgaa ttctttcaag gctacttttt ggcggtcgtt caagcctgtc
    10621 ggtgttacga cattaacagt aacgtattgg tcaccaactg caccgccacg aaggctcggt
    10681 gcccccttac tacgtaggcg gaatttctta ccagtctgag ttccctctgg aataaccaat
    10741 tcaacatcac cgtgaacagt tggaatatct actgtatcac caagagccgc ttggacaaag
    10801 ttgaggttga gattgtagaa gatagtcgtt ccttcacgtt caaacttgtc gctagcttcc
    10861 acagaaacta ctacatacaa gtcaccataa ggtccaccgt taaagcctgc ttcaccttga
    10921 ccagcgaggc gaatttgttg acctgtttcc acaccagcag ggattttcac atgtacgcta
    10981 tgagcttgtt tctcatgacc tgttccatga caggttgtac atggatattt gatttctttt
    11041 ccttggccgt gacagacatc acaggttact tggcgacgca tcataccaag aggagtctgc
    11101 gtatcgacgt taatgacacc agcgccatga cagcgtccac aagtgactgg acttgtccct
    11161 ggcttagcac cagagccatt acatgtacga cagccagctt cacgatgata cttaacttcc
    11221 ttctcagttc cgaagatagc ttcttcaaag gtcaaattga cacgatactg gagatcatct
    11281 ccttggcgag gagcgtttgg attgcgcgaa gaaccgcctc cgccgaagaa acttgagaaa
    11341 atatcctcaa aaccaccgaa gccacctgcc ccattgaaac cgccgaaacc accatctcca
    11401 ccaaaaccac cattggcgcc tgcagcacca tactggtcat aggcagcacg tttttggtcg
    11461 tcactcaaag tctcataggc ttcttgaact tccttgtact tgtcctcagc accaggctcc
    11521 ttgttgatat ctgggtgata ttttttggaa agcttacgat aagccttttt gatttcgtct
    11581 gccgaagcgt ttttggatac ccccagacga tcataaaatt cagtattgtt cataattcaa
    11641 gatacaaagg gcgtcaaacg gacacagcca tctaggagtt ttgacgacgg acgcttatgt
    11701 cctagaaaaa actatctttt tggcacagtc cgtagcccgt gttcagttac gaacaccctt
    11761 attcctttct attgatgttc tgaatcgaac caccatttag gtcggattca attcctttct
    11821 tttatattta gtaagaaatt ctgaaacccg tgttcagtta cgaacactct ttgttccttt
    11881 ctattgatga gacaaaaccc aaacaaatga gattccgtcc ctttttatcg aaaaacagag
    11941 gctgggttgc aacctctgca aataagtaaa ttaactttat tttgatttta aatatcctat
    12001 aagtacagaa atatttatat atttttgtct cttcttagta tttgcctata taattccaat
    12061 tgatcataag aaatacaact actgtccaaa tcataccaat tatgaacggt attacaaatt
    12121 tatatgtttg tatttctaat gatatcatag ctgataccat cgccgataca aatgcagaca
    12181 acaatactga agtaacaaaa ttccttaccg cttcatcatt tttgacacaa ctatcatgct
    12241 cagctaatat aatttctcta tatatagtca aacattctac tggaatatta tctataaaac
    12301 cttttgctgc ttcatagttg tcagcttgat tacaaaaatc attataaact tttcgtgtat
    12361 tttttagata ctcatcatcc aatacactca tcttattttt ccgtaaactc tccgtctacg
    12421 acgtcatcgc ctgcgtttcc tgttgcttgt gcgccttctg ctccttcttg agcttgttgc
    12481 gctgctgcgg cttgttcgta gagtttcaca gcaaggcctt gagctttttc gttcaacgct
    12541 tcaagtttcg ctttcatgtc gtccaagttg ttgtcttctt gagctttctt aaggtcatca
    12601 agggcagctt gggcagcgtc acgttctgcg tcgaagcctt taccttcagt ttccttgatt
    12661 gtcttttcag tcgcaaagat tgcttggtct acttcattac gaaggtctac ttcttcttta
    12721 cgtttcttat cagcttcagc gtttgcttct gcatctttca tcatgcggtc gatttcttcg
    12781 tcagtcaaac ctgagttcga ttggatgaca atagtttgtt ctttttgagt tccaaggtct
    12841 ttggccttaa cagacacgat accgttcttg tcgatgtcaa atgttacttc gatttgagga
    12901 attccacgag gtgcagctgg gatatcagtc aattggaagc gtccaagagt cttgttatct
    12961 gctgccattg ggcgttcacc ttgaagaacg tggatatcaa cggctggttg gttgtctgct
    13021 gctgttgaga agacttgtga tttagatgtt gggattgtag tgttgcgatc gataagtttt
    13081 gtaaatactc cacccattgt ttcgatacca agtgacaatg gcgttacatc aagaaggaca
    13141 acgtccttga catcaccagt aatcacacca ccttggatag ccgcacccat agcaactact
    13201 tcatcagggt ttactgattt gtttggttct ttaccagttt cagctttaac agcttcaaca
    13261 acggcaggga tacgagttga accaccaaca aggataactt cgtcgatttc tgacaagctc
    13321 aaacctgcat ctgaaagggc ttgacgaact ggaacttttg tacgttcaac aaggtcacga
    13381 gtcaaatcgt caaatttcgc acgagtcaaa gtcatttcca agtgaagagg tccagcctca
    13441 cctgcagtga taaatggcaa gctgatttgt gttgaagtta caccagaaag gtctttcttc
    13501 gctttttcag ccgcatcttt caaacgttgc attgccatct tgtcagtaga caagtcgata
    13561 ccgttttctt tcttgaattc tgctaccaag tggtcaatga ttttttggtc aaagtcgtca
    13621 ccaccaagtt tgttgtcccc tgcagttgac aatacgtcga agacaccgtc acccaattca
    13681 aggatagaga cgtcgaatgt accaccacca aggtcaaata ccaagatttt ttcttctttg
    13741 tcagtcttgt ccaaaccata agcaagagct gctgcagttg gttcgttaac aatacgttct
    13801 acttcaagac cagcaatttt accagcgtct tttgttgctt gacgttgagc gtcgttgaag
    13861 taagccggaa ctgtgataac agctttggtt actttctcac caaggtagtc ttcagcgtag
    13921 cctttcaagt attgaaggat catagctgag atttcttgtg gagtgtattc ttttccattt
    13981 gcagaaactt tttcagaagt tcccatctta gatttgatag agataactgt atctgggttt
    14041 gtaactgctt gacgttttgc agcatcacca acgatgattt ctccgttttt gaatgagact
    14101 acagatggag ttgtgcggtt tccttctggg tttgcgatga ttttgctttc agttccttca
    14161 agaactgcaa ctgctgagtt tgttgtacct aagtcaatac cgataatttt agacatgtgt
    14221 ttttctcctt gttagttaat tttctatttt tctttttgat actcttctta agtggcgacg
    14281 ccgtcagagc ttgacttcgt caatctcttt gactaaactt tgagcctaag gtctcaaaat
    14341 ttgcgcaaat agcaccacgg cgaagagtat cgtctttggt tcgcttcgct cactattaca
    14401 aagccaaaca tattttatct ttcttcatag tttattgtcg tttcggacaa gttttctatt
    14461 atgtaaattg cgacacgagg agtcgaaatc gattttattt cgacgacgag ttagtaagga
    14521 agctaggcaa acgccatagc gattgccgtt ttctgacgag ttgctttagc taccgtcaga
    14581 attgccgaat cgaacacgcc ctaagctctg agtaaaaaag atagattttc ttgtgttcat
    14641 cgaacacttc gtcaatctcc tatttttact gcgagctttt tacgagcttt gtatcttagt
    14701 tatacaccac taccattgct gggcgtagga tgcggtcatg gagtttgtag cctttttgaa
    14761 agacttgggc gatggtatct actgggtgtt catcgtctgc tgggagagtt tggatggcca
    14821 tatggtagtt atggtcaaat tcgccatctg ctgcgatttc ttcaattcct tcttctttca
    14881 aagcgtgaat caagctttct tgcaccatcg ccaagccctt cttcacatca tctgtcaaac
    14941 cttcaactgc aagtgcacgc tcaaggttgt caagagatgg taagattgct tttgccaagt
    15001 cctggctacg ataacgttgc aagttttgac gttcttcatt ggcacggcgt tggatatttt
    15061 gcatttctgc atgagcgcga agatatttgt tttcgaactc atctgcacgt tcatttgcca
    15121 agtccaactc agacttttca ggagttgttt cttcagctgt ttccacaact tcctcttctt
    15181 gaacttcttc tacttcttca ttttttatat cttgggccat gttcgcctcc tttaatgatt
    15241 tcaatcttaa tgtacttcgt aatgattact gctgaggtag cggtaaaaat ctgtcaactt
    15301 catggtcaaa acacggttga ccacattgac ttgattgatt agctgttggt aatccagatt
    15361 aactggaccg ataatggcta gaattccaac tccccgataa ggaatgagga acttactact
    15421 aatcaccgct aggtcagcta aacaggactc ttgaccgtct gcaacacgaa cattttgcat
    15481 ctgatcctca cgcaacccct cacgaatctc caaggccacc ttttgcggtt ggtcaaagaa
    15541 ctgataggct gctagattgg caaaattcaa gagattgacc ttgcccgcca tcacaatgtt
    15601 ttcgttgaac atttccttaa agatgtgttc aaagagatcg atgacattat ccgttgttgt
    15661 aaagtaacgc tggataatct gcggaatctc cgtccgaatc ttgtagtgaa tatctaaaac
    15721 ggtgtgaccg aggaaacgtt cctgaatgat tctcttcagt ttcagcaaat cctcctgcaa
    15781 gaagttcctt ggaatcagaa actgactagt aaccgttcgc gactcgtcta gggtaaatac
    15841 cgccaaggct gtatgttgcc ccaaaacaac gatatcaaag gctgtcaacc gttgcctgct
    15901 cggctcaaca tccagtgcca ctaccgtaca gccactcagg tctgttagca agttagcagc
    15961 ctcttgcaga atatcctcca atttgaagaa ttcctgatca aaggctttga caatctcata
    16021 tacctcattt tcagccagtc ggtcaaaatc cagtgagtgt ttcacatagt actgaaaacc
    16081 agcaacactt ggcatccgac cgcttgaagt atgagccttc tcaagcaacc cttgtttttc
    16141 tagttccgcc atgtcattac gaatggttgc actgctagag ttaatagact cttgcaaggt
    16201 ttttgatccg acaggttcgt gcgttttggt aaagatgtca ataatcagat ttaaaatatc
    16261 ctgctgacgc tctgtaacca tctcatcact cctctctgtc tgatcaatta gcactcaatg
    16321 catccaagtg ctaactcatg attctattat acacccttag aagagaatgt caagacaaaa
    16381 actcaaaaaa ttagcactct tttatcaaga gtgctaaaaa tcaatctgaa gacctagaac
    16441 cttgctattc atctactcgg tatttccttt ttagtctgaa ataaccatga attagataag
    16501 aaatcagaaa agcatataaa acaaaaataa gaaagaaaga catagaaaga tcctctctaa
    16561 acaaactcat acaaacaaca agaccaccta agaaagtagc tgtacaggaa cgaagtctag
    16621 atcgcataac ttcccatttg agccccttac tcatatagac ttgatcctct ggaagtaaat
    16681 cagaagacga tttacgaaga atcgaacaag aacctgctcc tatcaattcc caacctctat
    16741 ctctaaaatc ttgcagttca cgcttatgct ctttaagaaa tctagaatca tagatacgat
    16801 agataacatc gtccggttga ccttgctcaa aatagaacaa accaaaacga ctcgttctat
    16861 acctccaacc tttcaaatgc atctcatgta aatattcttc tttcttgtcc aaatcaacaa
    16921 tggtgaaaat ccgaaattct actctgctat tcattgtctt accccaaaat tagaaaacat
    16981 gcctggcgtt atttagttag ataattcttt ccacttttga ctcaatctcc aaaaaatata
    17041 agaaatctga atcgcaaaaa ctatcaataa aacccaatct attatgaaaa tcaaaaacac
    17101 tttccaactg aaagaactac ctccagtaac aaactttgag aaaaacggta gtagagctaa
    17161 aaagagaagt aaaataggaa gcatccgcat tgttaaaatc cgtttggcat aaaaaaatct
    17221 ttatttaaac gaaaatatta tagcaaaatt tacgccagtt tttgaacggc tgatgtagat
    17281 attttatact ttcaaaatgt ttaaatgtga ttatttattt ttgaaaaata gctcaccagc
    17341 ccgactgaaa gtgcttatag aatgataata agtcgcctgc cgaaaacagc ggaaaatagc
    17401 ggtgttatgc ggagataatc tgacgcgatg cgaaagtata ttgcatactt attttcaaca
    17461 atttagcaga gtatttttat aagtgtgata taatagaagt ataatttgtt ctgatagttt
    17521 attttatgga gaagtagatt tttagaatgc ggagggttca atatggttga gtttataaag
    17581 tctaagaaag aaatgagtga ggaggatatt aaagcaaatt tcatcactcc tgctattgta
    17641 tccaaaggat ggaaaaatgg tgagcatatc gcttacgaag aatacttcac tgatggtcga
    17701 attgaggtta gaggagataa ggctcgtcgt aaagaaggaa aaaaatcaga ctattcactg
    17761 tattaccaat ttggaactcg aattgcaatt gttgaggcaa aggataataa acacagcgtt
    17821 cgagcaggat tacaacaagc tattgaatat ggagagattt tagatgttcc atttgtttat
    17881 tcttcgaatg gtgatggctt tattgaacac gaccgtatca cgagagaaga acgtgagctg
    17941 gagttagacg aattccctac tcgtgaagaa ttattttctc gtatgacgaa ggaaaaagga
    18001 ttgacgtacg aaattacaga agctatctca actccatact atacagacgc cttctcaatg
    18061 aaaacgccac gctattatca gcaaatagct atcaaccgta ctattgaaac agttgccaga
    18121 ggacaaaaac gagtaatgtt tgtgatggca acaggaacgg ggaaaacgtt catggctttt
    18181 caaattattc atcgccttcg aaaagctggt ttggctaaac gagttttatt cttagcagat
    18241 agaaacatct tagtagacca aacgatggct gaagacttta agccattcga aaaggtaatg
    18301 acgaaaatta caccaaaact tttgactgct cctgaaaagt taaattcttt tgaaatttat
    18361 ctagggcttt atcagcaact aactggtgaa gatggaactg aaacacatta tcaaaaattt
    18421 gacaaagact tctttgattt aatcgtaatt gatgaagcgc accgtggttc agctaaggaa
    18481 gacagtaact ggcgtaagat aattgattat ttcagttctg cgacacagat tgggatgacc
    18541 gctactccta aagaaaccaa gaatgcttcc aatacggaat actttggtga gccaatctat
    18601 acttatagtt taaaacaggg aatcgaggat ggttttttgg ctccatatcg tgttatgagg
    18661 gttaatttag atgtggatgt ggatggttat cgtccagaaa ctggaaaagt tgatgctaac
    18721 ggacaattaa tagaagatag gtactacggc aggaaagatt ttgataaaac cattgtcatt
    18781 gatgatagaa cgcaaagagt tgccaagttt gtttctgatt atatgaagca aaacaatgca
    18841 cgatttgata aaacaattgt tttttgtgtt gatattgacc atgccgagcg aatgcgtgct
    18901 gcacttgtaa aagagaatct agacttagtc caagaagact atcgttatgt catgcaagta
    18961 actggtgaca acgctgaagg aaaagctcaa ctggataact ttatggatgt caattctaat
    19021 tttcccgcta ttgtaacaac gtctaaatta ttaacgacag gagttaatgc taaaacatgt
    19081 cgtttgattg ttttagactc taatatccaa tccatgactg aatttaaaca aattattggt
    19141 cgtggcacac gtctttatcc tcaaaagggg aaagaatttt ttacgattat tgattttcga
    19201 aatgttacca atttgtttgc tgaccctgat tttgatggtg atccagtgaa ggtgctagaa
    19261 acaggtgcga aaacagtcag tggttctacg cccggtttcg tagatgagga aggtgaccca
    19321 gtagaaaaat atatcgttac agacaagcag gttaccattc ttaattctac tgttcaagta
    19381 ttggatgaaa acgggaaact gattaccgaa agcctgaccg actacactcg aaagaatatc
    19441 ttaggtagct acgccacttt gaacgatttt atcacagttt ggcatacggc agataagaag
    19501 aagcttatct tagacgaact ttataaaaaa ggagtttatc tagatgctat tcgagagtcg
    19561 gagggaatat cagaacaaga aatcgatgat tttgatttac tcctaaaact tgcctatggt
    19621 caaaaagaat taaccaaaac ggaacgtatc aataaactca aacaaagcgg atatttatat
    19681 aaatatagtg aggaagcgcg tgctgttttg gaaattttac tgaacaaata catggataaa
    19741 ggtattggag aactcgaaag cattgaaaca ttaaaacttc cagaatttca gatatatggt
    19801 ggaaccttca aaatcatcaa tacttatttt ggagataaaa aacgatattt acaagcaatt
    19861 aaagaattgg agcaagagct atttacagta gcttaatgaa aggaaagtat gtcaattaca
    19921 tcatttgtaa aaagaattca agatatcact cgaaacgatg ctggtgttaa tggtgatgct
    19981 caacgtattg agcaaatgtc ttggttatta ttcttaaaaa tttatgatag ccgtgaaatg
    20041 gtttgggaat tagaagaaga cgagtatgag tcaattatcc cagaggaatt aaaatggcga
    20101 aattgggctc atgctcaaaa tggggaacgg gtattgacag gcgatgaatt acttgatttt
    20161 gtcaataaca agttattcaa agagttgaaa gagcttgaaa taacttcaaa tatgcctatt
    20221 cgaaaaacga ttgttaaatc agcttttgaa gatgcgaaca actatatgaa aaatggcgtc
    20281 ttgttacgcc aagtcatcaa tgttattgat gaagttgatt tcaatagccc tgaagatcgt
    20341 cattcgttta atgatattta cgaaaaaatt cttaaagata ttcaaaatgc tgggaactca
    20401 ggagaatttt atacgccacg tgcagcgact gattttattg ccgaagttct tgacccaaaa
    20461 cttggagaat caatggcaga ccttgcttgc ggaacaggag gcttcttgac ttcgactctg
    20521 aaccgtttaa gtagtcaacg taaaactagt gaagatacca aaaaatataa tacagctgtt
    20581 tttggtattg aaaagaaagc atttcctcat cttttagcag ttacaaatct gtttcttcac
    20641 gaaattgatg accctaaaat tgttcatgga aatactttgg agaaaaatgt tcgtgaatat
    20701 acggatgatg aaaaatttga cattattatg atgaatccac cttttggagg gtcagaatta
    20761 gaaacaataa aaaataactt tccagcagaa ttacggagtt ctgaaacagc tgatttattt
    20821 atggctgtca ttatgtatcg tttgaaagaa aatggtcgtg ttggagttat tttacctgat
    20881 ggttttctat ttggtgaagg tgtaaaaact cgcttgaaac aaaaactggt agatgagttc
    20941 aacttgcata cgattattag gttgcctcat agtgtctttg caccgtatac aggaatccat
    21001 acgaacattc ttttctttga taaaacaaag aaaacagaag aaacttggtt ttatcgttta
    21061 gatatgccag atggttataa aaatttctcg aaaactaagc cgatgaagtc agaacacttc
    21121 aatcctgttc gtgactggtg ggaaaatcgt gaagagattc tggaaggtaa gttctacaaa
    21181 tctaaatcat ttacacctag tgaattggct gagttgaatt ataatttaga ccagtgtggt
    21241 tttccaaaag aggaagagga aatcttaaat ccctttgagt tgattcagaa ttatcaagcg
    21301 gaaagagcaa ctttaaatca taagattgat aatgtattag ctgatatttt gcagttgttg
    21361 gaggacaaat aatgacacca gaacaactta aagcaagtat tctccaaaga gcgatggaag
    21421 ggaaattagt gccgcaaaat cccaatgacg aacctgcaag tgaattatta aagagaatta
    21481 aagctgaaaa agaaaaactt atcagtgaag gaaaaatcaa acgagataaa aaggaaactg
    21541 agatatttcg tggtgatgat gggaaacatt atgggaagtt tgctgatgga agtactcaag
    21601 aaattgatgt tccttatgat attcctgata cttgggagtg ggtgaggttt tctacattgg
    21661 ttgaaattgt cagaggtggc tctccacgac caatcaaaga ttatcttact tctgaagtag
    21721 atggaataaa ttggataaaa ataggtgata ctgaaaaggg tgaaaagtat ataaataatg
    21781 ttaaagaaaa aatcaaaaaa tcagggctta acaaaactag atttgtaaaa aaaggtacat
    21841 ttttgttaac taattctatg agttttggta gaccttatat tttgaatgtt gatggtgcaa
    21901 tacacgatgg atggttggct atttcgaact atgaaaactc attaaataaa gattacctat
    21961 tctatattct ttcatcaaat gtagtttatt ctcaatttct atctctaatt agtggagctg
    22021 ttgtgaaaaa cttgaatagt gataaagttg cttctattct tatccctctc cccccactat
    22081 ccgaacaaca acgcataata gaagcaatcg aatcagcttt agaaaaagta gatgaatatg
    22141 ctgaaagtta taatagacta gaacagctag ataaagaatt tccagataaa ctaaaaaaat
    22201 ctattcttca atatgctatg caaggaaaat tagttgaaca agacccaaat gatgaatcag
    22261 tcgaagtttt acttgaaaaa atacgagcag aaaaacaaaa actctttgaa gaaggcaaga
    22321 ttaaaaagaa agatttggac atttctattg tttcccaagg agatgataac tcttattatg
    22381 aggaagtacc ttgtgaaata ccagaaagtt gggaatgggt gaggttaaac gatataacct
    22441 cctacattca aagagggaag tctccaaaat attcaaatat tcctatatac cctgtgattg
    22501 ctcagaaatg taatcaatgg tctggctttt ctattgactt agcaagattt attgatcccg
    22561 aaacagttca tagttaccag aaagaaagat tactaagaga tggtgattta atgtggaatt
    22621 caacaggtct aggtacgtta ggacgattag ctatttacca tgaaaataaa aatccttatg
    22681 tctgggctgt tgcagatagc catgttacag ttataagagt cttatcggga gttattaatt
    22741 gccatttcat ctataatttt ctttcctctc ctattgttca gtcagttatt gaagaaaaag
    22801 cgtcgggtag tacaaaacaa aaagaattat taacgaaaac tataaaagaa tatttaatcc
    22861 ccctcccacc ccttcctgaa caatccagaa tcgttgataa aatcgaacaa tttttcgccc
    22921 atattgacgc actaatttag tataatagtt ttacgtgcgg tggaatttct atcaaacata
    22981 agataggagt tttacgttga aagattttga attacatttg aagaaagctg gtttagctga
    23041 aaatactgtg cgttcgtacc tttatggtgt tcgatttttc ttggagaatt acgaactcaa
    23101 aatggaagat ttatttgaat acaaaggata tttattagac aattttaaac ctaaaactgt
    23161 caatttgcga ctacaaggag taaataaata tctggctttt attggtcatg atgatttgaa
    23221 attaaaattt gtcaaagtac agcaaaaacc gtttttagaa gatgtcatta gccatgctga
    23281 ttaccttttt cttaaacgta gtttgaaaaa agatggtatt ttaaaatggc attttgtcgt
    23341 ttggttttta ggtgcaactg gggctcgtgt aagtgaactt attaaactaa aagtagagca
    23401 tgtagaaatc ggttattttg acatttactc aaaaggtggt aaaattcgta gactgtacat
    23461 tcctaaaaaa ttaagaaata gttgtcttag ttggttagaa tcagaaaatc gccgaagtgg
    23521 ttatttattt ttgaataaat ttaacgaacc aattacagca agaggagttg ctcaacagtt
    23581 aaaaaattat gctgataaat acaaaatgaa tcctaaagta atttaccctc attcttttag
    23641 gcatttattt gctaagaatt ttttagcgaa gtataatgat attgccttgc ttgcagattt
    23701 gatgggacac gaaagtatag aaactactcg aatttatcta aggaaaacag ctactgaaca
    23761 acaaaatatt gtagataaaa ttgttaattg gtaaaaaata acaggtggtc aaactgacta
    23821 cctgctattt ttgtgattat ggctcttatt atgggaatat acctatgaat tgggttgtta
    23881 taaaaataaa agatattttt tcaatgaata caggtctttc ttacaagaag ggcgatttaa
    23941 gcattaataa taaaggtgtt agaattatac gtggtggtaa tattaagcct ttagaatttt
    24001 ctctgttgga taatgattac tacattgata cacaattcat ctcctctgag caagtttatt
    24061 taaaacataa tcagctaata acacctgtag caacctcttt agaacatatt ggaaagtttg
    24121 caagaatcga taaagactat gatggtgttg tggctggtgg atttattttc caattaacac
    24181 cattcgaaag ttcagagatt atttcaaaat ttctattatt taacttgtcc tctccgttat
    24241 tttataaaca attgaaagca ataactaaac tatcaggtca agctttatat aatattccta
    24301 aaactacact gagcgagcta ttaattccgt tagctccttt tgaggaacag gaacttatta
    24361 ctcaaaaagt tgagaaactt tttgaaaaag taaatcaact ttgaaaatga ttcttttcat
    24421 ctcttcatga ttagaaatag ggattaataa ttcggagata ctggtactat ttaatgtttt
    24481 ccctttgata gcatcttttg aatcacctaa agtagagata agtggcaaaa atatcattaa
    24541 gtaatctctg ataatatttt ctttattagc ataggggaat atcgatataa tggcttcatt
    24601 atgagtggca ggaatatcca atatggcaac ttttccaata gataatttaa aactcattaa
    24661 taaagttcct ttaggtgaaa tgtctatttt ctttgatttt aatgctaatt tagaaataga
    24721 ttctctcgta ttagttacat aaccagatat aggcatatct gatatagata cccaaggtat
    24781 ttcagttccc caaaaagtag cttcactgcg tggaggagtt tttcctattc tgaagttaac
    24841 taggctagca aatttaatat atctccatgc ttctgggatt tcatatatag gataagaggt
    24901 tgtttcgtct ttgttc
//
LOCUS       DAVKGC010000018        24885 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKGC010000018 DAVKGC010000000
VERSION     DAVKGC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.018"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            176..256
                     /locus_tag="VW049_000598"
                     /pseudo
     CDS             176..256
                     /locus_tag="VW049_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(483..641)
                     /locus_tag="VW049_000599"
                     /pseudo
     CDS             complement(483..641)
                     /locus_tag="VW049_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(914..1603)
                     /gene="rplA"
                     /locus_tag="VW049_000600"
     CDS             complement(914..1603)
                     /gene="rplA"
                     /locus_tag="VW049_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9431766.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1811..2236)
                     /gene="rplK"
                     /locus_tag="VW049_000601"
     CDS             complement(1811..2236)
                     /gene="rplK"
                     /locus_tag="VW049_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9431767.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2368..2505
                     /locus_tag="VW049_000602"
     CDS             2368..2505
                     /locus_tag="VW049_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431768.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(2545..3261)
                     /locus_tag="VW049_000603"
     CDS             complement(2545..3261)
                     /locus_tag="VW049_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9431769.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3264..3734)
                     /locus_tag="VW049_000604"
     CDS             complement(3264..3734)
                     /locus_tag="VW049_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9431770.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMNGHGLKGCGPVWWVPFEEMTAETCQVKPEEIKRLVECLSSEVDKLLEIKE"
     gene            complement(3736..4773)
                     /locus_tag="VW049_000605"
     CDS             complement(3736..4773)
                     /locus_tag="VW049_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9431771.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTNEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRIYMEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVTLA
                     KNQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLRSNLVD"
     gene            complement(4871..6196)
                     /gene="brnQ"
                     /locus_tag="VW049_000606"
     CDS             complement(4871..6196)
                     /gene="brnQ"
                     /locus_tag="VW049_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9431772.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6367..6459)
                     /locus_tag="VW049_000607"
     CDS             complement(6367..6459)
                     /locus_tag="VW049_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9431773.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(6502..6654)
                     /locus_tag="VW049_000608"
     CDS             complement(6502..6654)
                     /locus_tag="VW049_000608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9431774.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAYNLPLSQ
                     LADSYD"
     gene            complement(6814..7392)
                     /locus_tag="VW049_000609"
     CDS             complement(6814..7392)
                     /locus_tag="VW049_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9431775.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7446..8846)
                     /gene="pepV"
                     /locus_tag="VW049_000610"
     CDS             complement(7446..8846)
                     /gene="pepV"
                     /locus_tag="VW049_000610"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9431776.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8859..9464)
                     /locus_tag="VW049_000611"
     CDS             complement(8859..9464)
                     /locus_tag="VW049_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9431777.1"
                     /translation="MKFLELNKKRHATKHFTDKLVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            9635..10429
                     /locus_tag="VW049_000612"
     CDS             9635..10429
                     /locus_tag="VW049_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9431778.1"
                     /translation="MKKFSLLLAILPFLVACENQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQLQ"
     gene            complement(10611..11444)
                     /locus_tag="VW049_000613"
     CDS             complement(10611..11444)
                     /locus_tag="VW049_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9431779.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11434..13278)
                     /gene="uvrC"
                     /locus_tag="VW049_000614"
     CDS             complement(11434..13278)
                     /gene="uvrC"
                     /locus_tag="VW049_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9431780.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDETYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVLNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNITKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(13318..14019)
                     /locus_tag="VW049_000615"
     CDS             complement(13318..14019)
                     /locus_tag="VW049_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9431781.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(14098..15330)
                     /gene="murN"
                     /locus_tag="VW049_000616"
     CDS             complement(14098..15330)
                     /gene="murN"
                     /locus_tag="VW049_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9431782.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQDLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEEKLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15335..16555)
                     /gene="murM"
                     /locus_tag="VW049_000617"
     CDS             complement(15335..16555)
                     /gene="murM"
                     /locus_tag="VW049_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9431783.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMIWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(16667..17446)
                     /locus_tag="VW049_000618"
     CDS             complement(16667..17446)
                     /locus_tag="VW049_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9431784.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17515..19176)
                     /locus_tag="VW049_000619"
     CDS             complement(17515..19176)
                     /locus_tag="VW049_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9431785.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19351..21573)
                     /gene="recJ"
                     /locus_tag="VW049_000620"
     CDS             complement(19351..21573)
                     /gene="recJ"
                     /locus_tag="VW049_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9431786.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21850..22608
                     /locus_tag="VW049_000621"
     CDS             21850..22608
                     /locus_tag="VW049_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9431787.1"
                     /translation="MALVEFKNVEKYYGDYHAFRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLETVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKAVLENVT
                     LAPIEVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            22648..23412
                     /locus_tag="VW049_000622"
     CDS             22648..23412
                     /locus_tag="VW049_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9431788.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKSSSHTAD"
     gene            23425..24102
                     /locus_tag="VW049_000623"
     CDS             23425..24102
                     /locus_tag="VW049_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9431789.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAVGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            24112..24771
                     /locus_tag="VW049_000624"
     CDS             24112..24771
                     /locus_tag="VW049_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9431790.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVT
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
BASE COUNT         7044 a         5801 c         4636 g         7404 t
ORIGIN      
        1 tacagttaca gatatttttt tcatggcatc tttgtcaact aaagaaacac taagttttac
       61 ggctccatga ctataatcat ctgtatgacc aaccttaact atgggatata atatatcgaa
      121 ggtaaatgtg aacaaactga ttagggaatt taaaccaact tctaacaatg tgttatactc
      181 aatgaaaacc aaagagcaga ctaggaaact agccgcaggt tgctcaaaac actgttttga
      241 agttatggat agaactgacg tagtcagctc aaaacactgt tttgaggttg tggatagaac
      301 tgacgaagtc agtaaccata cacacggcaa ggcgacgttg acgtggtttg aagagatttt
      361 caaagagtat tagaaagctc gatggactat tccagattca ttccactaga tttcgaaatc
      421 gagtagggga taatctctag cccctctcac accaccgtac gtgccgttcg gcatacggcg
      481 gttcaactaa cttttaacgc atgtcgttca aggtaataat ccaaacacga aaccagtcca
      541 cgtttttcaa ggactggttt tgatatagca cgtttaagta ccgacttctg agctactaat
      601 tgatagtagt cgccccagcc agatacctta tccgctatat aggccttatt atcacgttcc
      661 tgttcgtaga gccacgattt cgctatccct tcctctgacg attcttattt ccagcgtcag
      721 acttaaaacg gtctatcccc agaccgtttt aatccgctac ctcacgatag tcagacttgg
      781 gaatcgctat tgggttcacc ggtagtaggt gcccaccgag gacttacacc tcagatatac
      841 gacatgcccg tcgtacatca aaaaaagttg agacatagcg tcttcaactt ttttacatta
      901 ctttaactac tgattaaagt gagtttacgt caactttgat accaacacct tgagtagttg
      961 tgatagtcaa gtttgttacg taagttcctt tagctgtagc tggttttgct ttttggattg
     1021 tttcgttgaa agctttaaag ttttcaacca atttttcagc ttcaaatgat actttaccga
     1081 tgattgcttg aacattacct gcacggtcag cacggtaagt gattttacca cctttagact
     1141 cttcaactgc tttagcaaca tccattgtta cagtaccagt tttagggttt ggcatcaagt
     1201 tacgtggtcc aaggacacgt ccaagacgtc caacaagagc catcatatca ggtgtagcga
     1261 taactacgtc gaagtccaac caaccgtcgt tgattttagc aacaaggtca tcttcaccaa
     1321 caaagtctgc accagcagct tttgcttctt cagcttttgc accacgtgcg aaaacaagaa
     1381 cacgtgaagt tttaccagta ccgtttggca ataccattgc tccacggatt tgttggtcag
     1441 cttttttaac gtcgatgttc aagttgtaag caacttctac agttgcgtca aattttgcaa
     1501 agttagtttc ttttgcaagt gctacagctt cttctacgct gtatgctttt gtgctgtcga
     1561 ttttctcaag agcagcacga agttgtttgc tttttttagc cattttctat tctccttgta
     1621 agtggttcaa tcgattttca tctcccacgt cacttctcga aatgatgaag tcttgcgggt
     1681 tttcagtcta ctatttttct cttcctacgg ccttaagctc ccttgctgta cccaagtaca
     1741 aggctcagtc acttgcctag tatgaaaagc aactaataga ctgctgtgag attttctact
     1801 gtgttattga ttagtcaaca acagtgaatc ccatagaacg agcagtacct tcgatcatac
     1861 gcattgcaga ctctacgttt gctgcgttca aatctggcat cttagtttct gcaatttctt
     1921 gtacttgtgc acgagtaact gtagcaactt tagttttatt aggtgtacct gatccttttt
     1981 caacacctgc agcttttttc aaaagaacag cagctggtgg tgttttcgta acgaaagtaa
     2041 atgatttatc ttcgtaaact gagataacaa ctggaatgat cataccagct tggtcagctg
     2101 tacgagcgtt gaactctttt gtgaatccca tgatgttgat accagcttga ccaagagcag
     2161 gtccaaccgg tggagctggt gtagctttac cagcagggat ttgcaatttt acaagttttt
     2221 cgactttttt agccattttt aaatcctcct ttgtggtttt ggcggtaatt agagattttt
     2281 acctcccaca agtatgtttt tcacataccc atccattata cactatttat ctggaattgc
     2341 aagagaaaag tttatttttt gtactcaatg aaaatcaaag agcaaactag gaagctagcc
     2401 gcaggctgct caaagtacag ctttgaggtt gcagataaaa ctgacgaagt cagctcaaaa
     2461 cactgttttg aggttgtgaa tagaaccgac gcagtcagta ggtaaggcga cgctgacgtg
     2521 gtttgaagag attttcgaag agtattaatc gacgtagtct ccgccttcaa agccatagta
     2581 ttcttccaaa ctgagaccac ttgcagcaat ttctttagct tcttttccta cataacgcag
     2641 gtgccattct tcagccatat agcctgtttc cttttccttg cctttgagat aacggacaac
     2701 aaagccataa tcagctgcat gatccaagag ccattgggct gctttttctt ctgtcaccaa
     2761 atcaccatca gtcccaatca catcaaaggc caagcctgtc tggtgttcgc tatagccagg
     2821 acgggcagag taacggtcag ctgctgcctt tccatcttgg ttgacataat cttgatagag
     2881 cttggtctga gtttcataac ttctaaaacc actgtaatga tcactaatag ggaaacctgc
     2941 ctcttgcatc gctttgatga gtttgaccaa ctcagccttg gctgttggat tttccccggg
     3001 attatagtct ttcgacaaag gataatgttt attggctacg atgatttcgt cgtattttcc
     3061 ttggatgctg tagtaatcac ctttattgac cacttctgct tttttctggg cagccccttg
     3121 agacttactg ccgactgtcc catcagcttt ggctgtctgt tctgtcttag ctgtgccatc
     3181 ttcatttttt gctttttctt gtgaacaagc tgctagactc aaggctagca aggctgtcaa
     3241 gacaaggtat ctttttttca tttctactcc tttatttcta atagtttatc tacttctgac
     3301 gataaacatt cgactaatct tttaatctct tccggtttta cttggcaagt ttctgctgtc
     3361 atttcttcaa agggaaccca ccaaactggc ccacagccct tgagaccgtg gccattcata
     3421 tcacctcttc gtctagggaa aaggtgccag tggagatggg catcgccatt tcctagcagt
     3481 tcgatattca ttttctcagc agcaaatgcc ttggcaactg cctcttggac tagactcatt
     3541 tcttcgagaa aacggagtct tgtttccttt ttcaaatggt gcaattcggt aacgtgttcc
     3601 ttagctagaa aaagactata tcctgcaaaa tactggtggt ctccaatcac aagatagcct
     3661 gtttccaact ctttgacaaa gtagggattt tctcccttct tgatgagctc aattctctgg
     3721 caaataaggc acatatcaat ctactaaatt gcttctaaga taggcatcga ccattcgttc
     3781 tgttgcgacc accgagtcaa tatgagtgcg ctcataagaa tgactagact cgatacccgc
     3841 accaagaaga gcgtgtttaa cttctgcacc tgcagacata gccgctgaag cgtccgaacc
     3901 ataaaatgga tagatatcca gcttgaatgg aatatcttgg tttttagcca aggtcaccaa
     3961 atgttgacgg aagtcatagt gataaggacc tgaagcatcc ttgacacaga tggacactgt
     4021 gtactcatct gtttgctgat catcccccat agctcccata tccacagcca gatattcgac
     4081 tacttgagca ggaatattag aattagcacc atgccccact tcttcaaaga ctgaaaaggc
     4141 aaaatgggtt gttactggca actcaatctt ctcttccata taaatacgaa gcaggttaag
     4201 caaaatcgct gcgctgacct tgtcatccaa atgacgagac ttgataaaac ctgtctctgt
     4261 cacgacagtt cgtggatcaa aactgataaa atcaccgacc tcaatcccca aggcacgagt
     4321 ttctttttca ttggttactt tggcgtccaa acgcacttcc atattgtcct gggtacgttc
     4381 tgcagttcct gcatccttgt agacatggca agaagtttgg tggatgagga tggtgcctga
     4441 tactttttca cctgtgctag ccacatgaac ggtacagttt tcaccttcaa tcatgttcca
     4501 aggaaaacca ccgatacggt ccaatttaag acgaccatct ggtttgacag cacgaacaat
     4561 agcacccagc gtatccacat gagcagtcac atagcgatgt tgctcatcat tttgaccttt
     4621 gatggtcaca ttgacaccac ccttagctgt gcgaactggc tggtaaccaa aaccttctag
     4681 agtcttgact aaataattcg aaatttcacg agtaaagccc gttggcgatg cgatggctgt
     4741 cagttctttg atatattcta ctgtttgatt catttcttca cctctttcgc tacctattat
     4801 aacacattta tcatcggata aaaaaagaaa atcactcctg tagacttggc tacaaaagtg
     4861 atttaagtga ttattccatt tcaaaaacat cgctttcttg cttgtttggt agaaccaatg
     4921 agagcaagat tccaacaatg gctggcacca accatgggag agatgccttg gcaaaaggaa
     4981 gaacgctaac aagatttgca agaaactcaa ccttaaacga gcttcctagt acgcttgcaa
     5041 tggcaataac tgtaaccaca gcaattgtca actgcatacc tggttttgaa agggcaacaa
     5101 atttgttgac aatgacaatc ataacgatag caatcgtgat tgggtacaag ataaccagta
     5161 ctggaattga gtacttgata atcgcatcaa gacccaaatt ggcaatagca aatccaatca
     5221 aggtaaaggc tgtcgcataa accttgtagc tgatttgtgg gaagcgctca ttaaagaact
     5281 cagctgttga cacaatcaaa ccaacagtcg ttgtgaagca ggttacggta accatagctg
     5341 caaggaagag ttgagctgtt gagccaaaga tttcttgagt ggcttgtgac aagatgtaaa
     5401 cacctggtgt tccacccttc atcgcttcag ctggtactgg gaaatgattt ccaagaaaac
     5461 ctaaaccgat gtaaagagcg ctgaaggcaa gggcaacaac gataccaaca acccaaatag
     5521 ttgaaatgta ttctttctta cttgaaaatc caagttgttt caaggtttga actgcgatta
     5581 cgctaaaggc cactgaggca agggcgtcca aggtattata accttctagg aaacctgtac
     5641 caaaggcaga agcttgataa gcagctgaag cagcttgagg acttgttcca ccatatttga
     5701 tagctcccag aacgaccaag ataacaatca aaattgcaaa gactggcgtt aaaatacgtc
     5761 caatgcggtc taagattttt gatggattaa gcgaaatcaa ataggctgcc gcaaaataca
     5821 gaaccgtaaa tacaatcaag ccaagtcctt tatttgcatc cgacaaaagg gggctaatcc
     5881 ctacttcgta agctgttgta gcagtacgtg gggtagcaaa gaatggaccg attgacaagt
     5941 aaagaactga gaggtaaaga gtcgcaaacc aaggcgctat cttcgttgaa atctcgtaga
     6001 tatatccttt aggatttagc gttccaataa taagggtcaa gacggcgata ccaacgcctg
     6061 aaaagacaaa acctgcgatg gcaggaagaa aatgttctcc agatagagca cctagagaag
     6121 gcggaaaaat caagttcccc gcaccaaaaa atattccaaa caggagcaaa cctgttaggg
     6181 tacctttttt agccataaaa atctccttca tatttataaa atactgacct agtataccac
     6241 gattaggagt ctgaaaaaag catcacgagg caaatattaa atgttttcaa ttttcttaaa
     6301 aataagaatt atctaaaaat aaaactctcc tacccagtcc acaccaagta ggagaaatgc
     6361 aaaattttaa agttcttcaa aggctaacat gccaccctgg acattgataa cattataacc
     6421 ttgttctaat aggaattggc aagcacgcgc cgatctcatt ccagatttgc aaataatata
     6481 atgcaagcga tctttgtcca attaatcata actatcagcc aattgactaa gcggtaggtt
     6541 gtaggcacct tctaaatgaa gagctgcaaa ctcatccact tctctcacat ccactaaaga
     6601 aagctggtcg ttttggtaaa gctggtaaaa tgcgtcaaag gctatttctt tcattctttt
     6661 tcttctaata ctcttcgaaa atctcttcaa accacgtcag cgtcgcctta ccgtgcgtat
     6721 gttaccgact tcgtcagttt tatctgcaac ttcaaagctg tactttgagc agcctacgac
     6781 tagcttccta gtttgctctt tgattttcat tgactataaa atggttttaa ttcttttttt
     6841 caaatctggc actacttctg cctcaaacca aggatttttg gccatccaga tttgatttcg
     6901 tggtgatggg tgaactagcg gaaaataggc tggcagatag tctttatagt gtttcaccct
     6961 ctccgttacc ttcccactga ttttctcctg taaatagtag gcttgggcat attgcccaat
     7021 caagagggtt aactgaatat caggcaattc ctgtaagagc tgcggatgcc atttttctgc
     7081 aaaacctgta cgaggcggaa gatcacccga cttgccatgt cctggaaagt agaaatccat
     7141 aggcaaaaca gcaaaataac ctgaattgta aaaggtatct tcatccacac ctagccagtc
     7201 ccgcaagcgg tcaccacttt tatctttcca gtaaaggcct gcttcttgag ttttaagtcc
     7261 cggagcctga ccgatgatat tgatgcgagc agtttttggc gctgcaaaga gaggctcaat
     7321 gccacgctct gtatagctgg cattctgcga atccgccatg atagcctgtt tgattctttc
     7381 gatttgagac atctactttc cctccaaaaa gaagtccaag cataagatct cagacttcta
     7441 tcgttttatt tgatcaattc gtaaatagct tcggcataaa ttgctgctgc tcggaagaga
     7501 tcgtccaagg caataaattc attggcttgg tgcatggtat caattgagtc tgggaacata
     7561 gcaccatagg caactccgcg ctctaacaag cgaccaaagg ttccaccacc gatgacttgt
     7621 tcatgacctt gtaggcctgt ttgtttttca tagacattca acaaggtttg cacaagtgga
     7681 tcttccattg gcacatagtg aggggtatga ccatgttctg atagactgac agaagcaact
     7741 ggcaagtttt ctaggattga tttgatttgt tctggacttg ttccttttgg atagcggatg
     7801 ttaagggcaa tggtattatc agcacttgtt tcatcgaagc ggaagactcc ggcattcata
     7861 gaaagagcac ccatcttttc atccacatga gcaatcttga gattttcacc ctcatgatcg
     7921 ttcaagagaa ttttacctgc gatgtcaagg tagtctttgg ctggaccagc aaagccaaac
     7981 tggctgagga agagggcaag gtaagttgcg ccattgacac ctgaagcagg catagcaccg
     8041 tgggctgatt taccaatgat cgtcaccttg tatttgccag cttcttcttg gagttctcct
     8101 ctaagtttgt gttctgcaac aaaggcatct agtttagctt gcaagtcagc caaatcacct
     8161 gaaacgactg ctgttgctga ttctggtacc atattttcac gtaaaccacc tgtaaagctg
     8221 tgaagacggg cagcacctgt attttctcct gcaaagtgga ggtattccgt gatatttcct
     8281 ttttcaccat tgatgattgg aaattcagca tctggtgaga aaccgaaatc tggtttggca
     8341 agtcctacgt gctcaaagta gtagtccatg tctgcccagc ctgattcttc gtctgttcca
     8401 acgatgaagc gaactttctt agaagttgga agacccaatt ctttgatgat tttcaaacca
     8461 tagtaacaag ctgttgtagg acccttatcg tccgaagccc cgcgcgcata aaggcgacca
     8521 tctttgatag ttggtgtgta agggtctgtg tcccaaccgc taccagcagg caccacatcc
     8581 atatgggcaa agattccgag aacttcttct ccatcaccaa actcaaaatg tcctgcatag
     8641 ttatcaacat tcttagttgg gtagccatcg cggtctgcga tttcaaggaa tttctccaag
     8701 gcttttactg gaccaggccc aaatggatgc tgggcatcag ccttgctgtc atcacgttct
     8761 gaattgattt ccaaaaggct aaacaagtca gccaagaggt cttctttgcg tttttctact
     8821 tctgctgtaa aatcaattgc tgtcattttt tcttctttct atcttttctc gatgatttca
     8881 tccactggca agcggtagct tggttccaat ttttcgtctg tataacccac tgtgatcaag
     8941 agttctgggc ggaaacggtc ttcgatttcc aaaacttcat taacttttga tttgtcaaaa
     9001 ccaagaataa tgttagaacc aattccttgg tctgtcaatg caagaaccaa gttcatggca
     9061 accaaacctg cattgagagc taggtagtcg ctgacttgtt gctcactgta acgggcaaac
     9121 tcagctggca gatttttcat aaaatattga agttgctctt cagaaaagtt attagcacca
     9181 ccaacacggg caatcttacg agcacgtttg gctaagtccg tatctgtaaa caaggcaatg
     9241 gttacaggcg ctgatgatac ctgttcaaaa ttggaaccat aagctaactt tgccagttca
     9301 gcatttttct cacgtaccac cacaaatttc caaggctggc tgttgtgggc gcttggcgcc
     9361 aaggttgcaa tttcgatagc cgtacgcaca tctttgggat caacaagctt atcagtaaaa
     9421 tgcttagtcg catgacgttt tttatttaat tcaagaaatt tcataatcga tttccttttc
     9481 taattctact gcttccattc taccataatt tgaaagagct tgcagggatt tttcatgatt
     9541 aagtttttct atcacagaca taaaagatat atattggttt atcaggaaag tttctgataa
     9601 actagcaagt actttacatt tgaatggaga cagtatgaaa aaatttagcc tattactagc
     9661 tatcctacca tttttggttg cctgtgagaa tcaagctaca cccaaagaga ctagcgctca
     9721 aaagacaatc gtccttgcta cagctggcga cgtgccacca tttgactacg aagacaaggg
     9781 caatctgaca ggctttgata tcgaagtttt aaaggcagta gatgaaaaac tcagcgacta
     9841 cgagattcaa ttccaaagaa ccgcctggga gagcatcttc ccaggacttg attctggtca
     9901 ctatcaggct gcggccaata acttgagtta cacaaaagag cgtgctgaaa aataccttta
     9961 ctcgcttccg atttccaaca atcccctcgt ccttgtcagc aacaagaaaa atcctttgac
    10021 ttctcttgac cagatcgctg gtaaaacaac acaagaggat accggaactt ctaacgctca
    10081 attcatcaat aactggaatc agaaacacac tgataatccc gctacaatta atttttctgg
    10141 tgaggatatt ggtaaacgaa tcctagacct tgctaacgga gagtttgatt tcctagtttt
    10201 tgacaaggta tccgttcaaa agattatcaa ggaccgtggt ttagacctct cagtcgttga
    10261 tttaccttct gcagatagcc ccagcaatta tatcattttc tcaagcgacc aaaaagagtt
    10321 taaagagcaa tttgataaag cgctcaaaga actctatcaa gacggaaccc ttgaaaaact
    10381 cagcaatacc tatctaggtg gttcttacct cccagatcaa ttacaataac actcttcgaa
    10441 aatctcttca aaccacgtca gctttatctg caacctcaaa gctgtgcttt gagcaaccta
    10501 cggctagctt cctagtttgc tctttgattt tcattgagta taagatatac taaaagcgat
    10561 tggaccagcc aatcgctttt ttagtttgca ttggctattt taggaaactc ttacaaaaaa
    10621 tcaaaaaaat tcttcacatc ctctacatac ccatgctttt ctattaagct ggctaaaagt
    10681 tccagattgt gcccctgata gttatcgtca cgacgcagtt cttcatacat ttcaataaag
    10741 ggaagcccct tggacttggc caattctaac tcagcctcgt aatcataagc aactttacga
    10801 aattggatat tgaccaattc cccatcttca acttctatca cggcatactg ggcacggtga
    10861 ttttttaacg cctcccaatt aaaatagggc atgccaatcg accctggatt gatgatttgt
    10921 tgcccttgac ttccataacg aagcaactgc ttgtgaacat gaccataaac tgccacgtcc
    10981 gtttccgcat ctagcagttg gtcaaatttc tctgtatcat tctcaactag caagtcacca
    11041 ccatagttct tatttggcaa attatgagag atagaaaagc gcagtccagc aatttctttc
    11101 ttttccagca aaggcaagct tcgtagccag acagtcgttg caggatccat tcgctccatc
    11161 aaaaactggg tcatacgcaa gagctgaact tcctgtgggt cttctaagcc atattgccca
    11221 tctaaagcct caaggacacg atcatcccaa ttgcctcgaa cacttgctgt gataggaagg
    11281 tcctttagca gggcgactaa gtcatttgcg cctggaccag gaagaaaaat atctcccaga
    11341 agccaatatt cactggcccc ttgattttta gcatctgcaa tcactgcttc tagcgccgtc
    11401 gcattgccat gaacatctga taaaattgcg attttatggt tcattgtggt ttccttccgt
    11461 ttggtaatct actctttctt ctgctacttg aggcaaggct tctccctgcg ggttcaactt
    11521 tctttgcaca gcctctgcaa cgactctagg taccccaact tcgacaatct catccacact
    11581 ggcttccttg attttggtca aagacttgaa atgcttcata agattctgct tgcgtttagg
    11641 tcccagaccg tcaatcccat ccaattgaga tgagaaagaa tttttggagc gcagttggcg
    11701 gtggaaggta atggcaaagc ggtgcacctc atcttggatg cgttggagga ggaaaaattc
    11761 ctgagaattg cgagacaagt ccaccacctc aagcggatct ccaaagagca attcatgggt
    11821 ttggtgctta tcattctttt gcagcccagc aattggaata tccaagccca gttcctcttg
    11881 gataacctgc ttagtgatat tgacttgacc ttgcccccca tcaatcacaa tcaaatctgg
    11941 aggagtcaaa gcctcacgct gtactcgacc atagcgtctg cgaatgacct ctctcatgct
    12001 ggcatagtcg tctggtccaa caaccgtttt tatcttgtac ttacggtaat ccttcttact
    12061 cggtttaccg ttgacaaaga ccaccatagc cgaaacaggg ctagttccca tgatattaga
    12121 gttatcgaag gactcgatac gtactggggt cgggatttgg agcaaacgtc ctagattttc
    12181 aatagctcct tgggtcttct cgacagattt ctctagcagg ttaaatttct gctccagact
    12241 aacacgtgca ttttttatgg ctagattgac cagctgtttt ttctctccac gttggggttt
    12301 gagaatcttg gtatccacca aagccttgac agcctcttcg tcaatatcct gcggaatcag
    12361 tacctcattg agaactaggt gagatttttc ttgatagaat tgtcctacat aggtcaaaaa
    12421 atcctcatct ggatcattga agtaggggaa gagattgaca tcgcgctcga tgagcttacc
    12481 ctgacggaca aagaaaacct gcacacacat ccagccctta tccacatagt agccaaagac
    12541 atcgcgattt tggagatcct tagccatgac ccgttgcttg gttcgaagcg ttccaatagc
    12601 ctgaatcagg tcacggtatt ccgccgcacg ttcaaactcc atagtctggg ctgccgctgc
    12661 cattttgccc tttagctcat caatgatttt gttatcctgc cctttcagaa aatcagaaac
    12721 ctcctgggcc atagacttga aataagtctc atccttctta cagatggtgt gggccatgca
    12781 ttgaccgata tggtaataaa aacagacctt agagggcggg ttggtacact tacgaaaagg
    12841 gaatatccga tccagcaacc gcttgatttc attggctgcc cccacatcgg gatagggtcc
    12901 aaaataaaga cctccgtcct ttttgacctg acgagtgata atcaagcgtg gatagcgctc
    12961 attggtgatt ttgatgaaag gataggactt gtcatccttg agcatgatat tgtacttggg
    13021 cttgttttcc ttgatcaggt tgatttctag gagaagtgcc tcaatattag actccgtaac
    13081 aataaattca aaatccacaa tttcagacac cagagcctct gtcttggtat catgacttcc
    13141 acgaaaatag gaccgtactc gattacgcag atttttagcc tttcctacat agataatggt
    13201 gccattttta tccttatgaa tgtagcaacc agggctggtc ggcaagagct ctagttttga
    13261 tttaatcaag ttgttcatag ttccattata gcaaaaaaag tagggaaaga tagtacccta
    13321 ctcactagtc tcatatattt ttcggaggtt tgcaacatcc tcttcctgga taccataaaa
    13381 ggaacctgct ggttgcatga cagacttctc atctgtatgg tccaagccaa tcgcatgacc
    13441 taactcatgt tctgccgtat ggacaaggcg ttcataggag tagccatagt atggattgga
    13501 caaataataa tgattcaacc gcaccgttac ggacaagaat tgccctgtta agagattagt
    13561 ttgactttcc gcctctcctg ccacaggagt gcctccgtcg ttcatctccg tagccgtaat
    13621 atccgcctta ctagactcag tcacgagttc aaagttaaaa gcacccgttt gattccagtt
    13681 ctggattgct tctaaataag cctcttgaaa gcgtgaatcc atctgcggat ccaggtaaat
    13741 acgagcagag gcctgtgccc accttccttc agaatgctgg tgactatctg tctggcttaa
    13801 cttaggcagt tgccctgttt tctcccattg gtcaatactt tgttgaccaa tttttactga
    13861 ccgttctgct tgctttagcg ctccttgaaa atccccgttc agataccaga gaagtccgaa
    13921 agcaagcaca cataagagca caactatcca aaccagacgc caaaacaaac gccacacaaa
    13981 agaaaagaaa gcccctatca aacgaaaaag ccagcgcatg tctctccacc tttctagcaa
    14041 ataagaatta ttttactaaa agaagctaaa agaaatctaa atactgactt tttcatctta
    14101 acgtcctact atttttttga gtaactggat agctttgtat tttaaaggcg atggatggta
    14161 acggaaagta cccgctttgc gtacaatata gccattaaaa ttctgtttaa aacgcaaaac
    14221 accatcactt ccatcaaaaa tcccttgaat gcctaggaag ttgtatttag gtattccacg
    14281 ttttatgctt tccaacataa catatttttg aagcagtgca ggggcataga acttattaaa
    14341 ctcagtgtag gaaccactaa agagataagt cgtttcctga ggcatataaa caaataaact
    14401 accagctaaa acaatatctt cttctccata tttttcaatc aagtctcgcg cttctgcttt
    14461 tcgaacttca aacgtttcaa attgactaga atattctctc agttgatttt gttttttctc
    14521 agaatgagga tttttactca aatcaagtcg caacttgtcc aatttttctt ctagtttact
    14581 ttgttcacct tgcaatttgc tcatatactc cgaaaaattc aagcttgcta tgagaaactc
    14641 cgcttgttct ccaaaagtat cataaaaatg ctcataatat tctaaacttt tatcactata
    14701 ttctctacgt tcagaggttt cttttgttat attcttaaaa atcgatagtt cttcacgttt
    14761 taactttttc aaccgaatgc caaaggtttc agcctttttc accaagggtt tacccttttt
    14821 gctaaaactt ttaagcaaac tcttttcagt taattcagtt aaatctttat agtataacca
    14881 atctggttct ccacctgggt aacctgttgt taagccatca aattgataac ctaaatcagt
    14941 caaatcttga ataatacttt ttttctcagc atctattgga ttaccttggc tatcaaaagt
    15001 ttgataagtt tcatagggtt ttacaagcaa ctctaataca ccattttgct tggcatattc
    15061 ttttaactct gcataaaaaa ctggaagagc atcttgttgg gtataaatcg gccccgaatt
    15121 gagttccata tgcagaccac ccagcatggg caggctataa accagagctg caacttgaat
    15181 ttctccttct tgtttcaaag caagataaac aattcgagcc cctctttttt ctagcaaatc
    15241 ccccatctgg acagattgca taaaggaacg agaagaaacc tgatcagaat aagtctgaaa
    15301 ctcttctttc gtgagtgttg ttagtgccat atacttactt tctatgtttt tttcttaatg
    15361 ttttacggaa atcaagagca agtcttaaca gaggatagag aggatgagtg ggcattgtaa
    15421 attcacccaa gtattcttca atcgttggat taaatttttc cttaaaatga taaagtccac
    15481 cattgagaga gttttcaaca ccacctaaat tttgccagat catacctcgt tcaaaggcat
    15541 agcgagccgt ttcataccaa gttaaaattg gtgcattgta acgtttaaaa tcatcatcca
    15601 taccagcata tatattgaca gaggtagtac caaattccaa actcaaagta gccgctaaag
    15661 gaactctcgc ttgacctaca tctatatatt cctgcaagaa ggtcaattcc tctaacaaac
    15721 gttctttttc cttcttctgc gcttctactt ttgaagttcg agtcgactca gtaaaggtct
    15781 cttccaaggc tctatttttc gctaactgtt cttctaactc ttgcgaacgt ttagaaacat
    15841 ccaaggtggc caaggtgata taggccttgt ccttaaaatt atctaacaat tttttataat
    15901 aggcttcatt cctcaaatga atctcttttc gcttctcagt ttttttcatc aactccgaaa
    15961 atgaatctaa tagttccagt ccaccatatt gaatctcaag ccctttgttt cgtgctgttc
    16021 gaatagcctg ttttgttgac ttggaaagtt tatcttcttc aaaattttcc ttgtatattt
    16081 tcgcctgaat acgaggttga atggtgtctc ccatttcctc cgtttttcct gaccacctta
    16141 ctcccatttg ttgcaaacta tcaataatag ccagattttc aggaaattct gtcttttcct
    16201 gattgattaa actttgagat aggcaaatac ttgggtcaaa agtcacaaaa accgctctct
    16261 tactgcgagc ataggactta atagactgaa tggcaaaatt caagagttct ttatccccat
    16321 aatccaatat aggtcctctt gggatgtaaa acattttata gcctagcgga agagttctaa
    16381 tcaaaatact agctgtcgcc agtaattttt cttccctgta aacaccaaac ttctcatgtt
    16441 gccaattaga cttaacttcc tcccaagcac tactttgtaa tacattggct aattcatgtt
    16501 ctttgacaaa ctgatcatat tctaatgtgg gaatgccaat ttgataacgg tacatttcct
    16561 actctctttc ctccagtatt ttattactat tctactactt tcgtttgaaa agtctagtga
    16621 aaacatattt tattttagtt ctactccccc tatgctgaag ttcaaactaa gtcagtctct
    16681 cttctaattt gaaatcaatt ggtagggttg ttaaaaaaac ttccaattgt ttttcccagt
    16741 agtaccactc gtgagttcca gcgctatggc tataggtcac atcaaaacct agttttttga
    16801 gatttttcac tgcgagatta ttggcttcgt acaagaaatc ctgttcgcca caccacgccc
    16861 aaagtttggt ctttttatcc gattttttag ccagactttc aagagaatag ggactagttg
    16921 tccagtctct aatctctcca aaaacacctc tccagtaggc tggacttccc agattttgac
    16981 tttcaggaga aaagttttga aagctgaggg cacctgaaaa actagctgca tgagaaaaac
    17041 gatttgtcgt aagagccagt ttgaagcagc cgtagcctcc catagaaaga ccagcgataa
    17101 aggtcttttc acgcttgctc gtcatattag ggaagaagcg tttcagaacc tgtggcaatt
    17161 cctctgctag agccgtgtag tagtcaaaac catactgggt atcggtgtac caaccattgc
    17221 tggtattggg cataacaacg atgagattag ttcctcgaag caagcgttct acattggtcc
    17281 gcttaagcca actattatga tttccagaca tcccgtgcaa aaggtacaag acgggaatat
    17341 cttcacactc tggttcttcc actcgattgg catcagggta gaggacattc accccccact
    17401 ccatatccaa tacttgtgag taatactcga ttttcattac tgccatgatt ttctccttct
    17461 atttttctat aagaaaaagc cgaaactcga ctttctctct gtttatttca acgattattt
    17521 tgcttccatg actactggta aaatggctgg gcgacgcttg gtttgatcaa agaggtactt
    17581 ggtcagattg tcacgaacct tacctttgag atctgcccag tcaaagtcat ctccttgaag
    17641 atactcttct accgtttggt taatcaattc tgaactttca cggagaatat cgcgactctt
    17701 cttgagataa acaaatccac gcgtgtgaac acgagcccta gccacaattt tcttctcacg
    17761 acggttgact gtaatagcca cgatgaaaat tccatcctct gacaagacct tacggtcacg
    17821 aagaacaaca tttccaacat caccaatggc attcccatca atcaagatat ctcctgctga
    17881 aaccgatcca gctggaacaa agtctccatt ctcgtaagcc atggtcgtcc cctttttagg
    17941 aatgaagatg cgttctggca acatcccaac tgccatggca gccttagcgt gagcatccaa
    18001 ctcacgatac tccccttgga cagggaagag gtacttaggt tgcaaaagat tgatcatcag
    18061 ctgcaaatca cgcacatttc cgtgccctga tacatgtaaa ctttgggtaa tcaatttgac
    18121 aaccccacct gcctgataaa tcatattttc cacacgcgca acaaaggctt ctttagcaat
    18181 agacggagcc gtagcaatat agactaggtc cccatccttg atttctacat aacgatggcg
    18241 accaatcgac atcttacgaa gtccattgat aggctcaccc atacgacctg tctcaagaat
    18301 aatcaactca tggtcttcaa agcgagacat atctttaggc ttaatcaaaa gaatttcgtt
    18361 ggctaaagac aacttcttaa gacgaatcgc tgtgcggacg atattttcaa tatcaaatcc
    18421 tgtcaagacg atacgtcgac ctgttttatc cgcagcgtca aaaatctgct gaatacgaga
    18481 aagattactg gaaacagctg caacgatgat acgaccttcc cagtcagcaa tagtttgggt
    18541 aatttcatcc ctaacttcac tttcactagc cacctgaata ttgctgtctg cattggccga
    18601 atcactgagg agagccagga cgccgtcacg accaatctct gccaaacgag caaaatcagt
    18661 tgcataagat tcactagccg tttggtcaaa tttgaagtca cctgtataaa cgatgcttcc
    18721 ttccgatgtc ttcaagacaa ttcccagact ctctggaacg gagtaagtcg tagggaagaa
    18781 ggaaaccact gtcccaccaa aatcaatctc cgtattctca tcaatgacat ggaaatcatt
    18841 aaatttctta acggcatcat ttcctttgac aaagagcttt gccaactcaa tggtcaactc
    18901 agacccaaat acaggaactt tagcctctgc caagagatac ggtagagcac caatggcatc
    18961 cgcatgcccg tgggtcaaga aaaccccagc aatacggtcg ctattttcaa aaaggtaatc
    19021 catgtttgga atcaccacat cgacccctaa ttgttcattt tcaggatatt ttaaccctac
    19081 attcaaaaca aaaatggact ctccaatttc agcaatgtac atattttttc cattctcacg
    19141 cacaccacca agtgttgtta aactgatatt actcattttt cctccaaaat cttagtttat
    19201 cttgattata gggcaattct accctcttat tctaaaagtt ctgatacttt cagccgaatc
    19261 ttttcctacc attataccat ttttttgatg attttcaaaa tgaaggtctg ttttcaaaaa
    19321 agagttgatt tcccaactct ttcctaactt ctactctttt tccatcaaaa aatcataaat
    19381 ttcttgcacc gtacccagcg ccatcatttc ttggtcttta acggtttgtt tgagattttg
    19441 gtaaatttga ctttctccta tctcccgctt tggcgcctct ttattgactg tcatcacacc
    19501 atcttttatc gtcacaaagc ctagttcttc aaatacttga atcatcttga ccagcaagat
    19561 ttgttgaata ttaagatatg cagccaaatc tttcagcttg tagcgaatat caaactctgg
    19621 gaactggtaa atagtcttgt acaatttggc aaactgatct ctagtcccat aacctgtcag
    19681 ataataagcc ttgtcaatat catttttgaa atagacagca gagaaatgct gttcctgaaa
    19741 aatggtcttc agctgagtaa tatcctctgg aatgtttttt acgacaacag cttcactagc
    19801 cgcaagattt ggcagttctc caggaaaatc caagactgga acaccttctg gcaagactgc
    19861 attttttcca cgaatgttaa aaagttgaac accttccact cgcgcatcca ccatcatcaa
    19921 ctggagggca gtttggccat tccattggtt gacagacaat ttaaccgcta actctagatt
    19981 cttggtttga gaaaactctg tcgcccatct gccttgacca aaggctacca cttcaaaact
    20041 cgcctcaccc ttggaaattt tcagctttag atgggcatta cctgccccca tagtacgagc
    20101 actttcgacc tgaaaattct tgatataaaa aataggtttc tgattatcca ttccaaaagg
    20161 agctaaacgt tcaaaacttt tgaccgtttc caagctaagt gcctccaaat ccaactcttc
    20221 atctaggttt aacttattct tgccaccagc atctgcacct ttttcacgaa cataatcttc
    20281 caaaacctga gataaatctg agagttgctc aacttccagc gtcatacccg ctgcacctgc
    20341 atgacctcca aaggcgatga agaggtctcg atggggatcc agagcttcaa aaatatcgac
    20401 cgcttccaca ctacgagcac tgcccttggc acgaccgtct tctatattaa gaacaatgac
    20461 tgtctgtccc aattcttcca ataaacgacc agccacgatt cctagaaccc caggattcca
    20521 gccttccttg gccaagacct gaaccttctt ctcaggatcc accatggtct tggcttcttc
    20581 atagatagac tgaacgattt ccttgcgctc ttcgtttttc tggtgaatca taagggcaat
    20641 ctcatgcgct tcctcatcat caaatccagt caacaaatca atggcaggat tgggatcatc
    20701 caagcgaccc aaggcattca aacgaggagc aatctggaaa ccaaccgttt cttctgttac
    20761 ttcgttggca gcaatcccag ccatgtccag catttcttgc agaccaatgc gctgggtatg
    20821 acccaacatt tccagaccat attgaactaa gatacgattt tcatccgtca gactcaccat
    20881 atctgcaata gttccaatag cgaccaaatc aagcaattcc acttgcactt cttctaacag
    20941 ggcacaagcc aacttgaaag caactccaca accagccaaa tatttaaaag gataatccgc
    21001 atctggatgt tcaggatgga caatagcata agcatctggc agggtttcag gcatggaatg
    21061 atggtctgtc acaatgacat ctactcccat agactgagcc aatgcaatag cctcatgacc
    21121 agcaacccca ttgtccaccg tcacaatcaa ggaaatccct tcttgctcga taaagtattt
    21181 ataaacacta gcattagggc catagccatc ggtaaaacga tttggcaggt aaactcggca
    21241 ctcagcacca agttgttcca aactttcctt cacaatagaa gccgaagtca tgccatccgc
    21301 atcatagtct ccataaacaa gaatattttc cccttcttca atagcctgac gaatccgctc
    21361 cactgccttg tccatatcat ggagcagata agcatcatgt aagtcctcca aggaaggttc
    21421 taaaaacttc ttcagacttt cttggttctg aatccctctc tcaaacaata accgagccac
    21481 ctcaggaccc agtccagcct tcttggctat ctttgtaaaa tccgcatctt ctacctgcag
    21541 ggcaaactgc cattcataag taggtgttat caaaaagaca tccactcttt ctaagaattc
    21601 tattgcttca ttataacatg atttagacgt tttctctatc cagattgctt ttttataaca
    21661 ttttatagta tattgaaact agaatagtac acctctactt ctaaaatatt gttagaaatc
    21721 gatttgactg tcccgatcga tttttcctgt tcttatttca ctatagtgaa ttttttcaga
    21781 tatgatttga caagaacatt cttttattgt agaaccgtgt tataaaatat aacacaatag
    21841 agatctctca tggctttagt agaatttaaa aacgtcgaaa aatattacgg agactaccac
    21901 gcattccgca acatcaatct ccgttttgaa aaaggacaag ttgttgtcct gcttggacct
    21961 tctggctctg ggaagtccac tcttatccgt acgatcaatg gtttagagac tgttgacaaa
    22021 ggaagtctcc tagtcaatgg gcaccaagtt gctggtgcca gccagaaaga tttggtacct
    22081 cttcgcaagg aagtcggcat ggtttttcaa cattttaacc tttatccaca caaagctgtg
    22141 ttagaaaacg taacgcttgc acccattgaa gttctaggaa ttgataaaaa agaagctgaa
    22201 aaaaccgccc aaaaatatct ggaatttgta aatatgtggg acaagaaaga ttcctatccc
    22261 gccatgctat ctggtggaca aaaacagcgg atcgccatcg ctcgtggtct tgctatgcat
    22321 ccggaactcc tcctctttga tgaaccaaca tctgctcttg atcctgagac tatcggagat
    22381 gttctagcag ttatgcagaa actggcgcat gatgggatga acatgatcat cgttacccac
    22441 gaaatgggct ttgctcgaga ggttgcggac cgcattatct ttatggccga cggagaagtt
    22501 ttagtagata cgacagatgt cgataacttt tttgacaatc caagcgaacc tcgtgcccaa
    22561 caattcctca gcaaaattat caaccacgaa agtgacaaag tcaaataagg aggcgcctat
    22621 gaaaaaaaat tctttttatc agcattattg attagccttt tcggccttgc tgctgccaaa
    22681 ccagtccagg ctgatacaag tatcgcagac attcaaaaaa gaggcgaact ggttgtcggt
    22741 gtcaaacaag acgttcccaa ttttggttac aaagatccca agaccggtac ttattctggt
    22801 atcgaaaccg acttggccaa gatggtagct gatgaactca aggtcaagat tcgctatgtg
    22861 ccggttacag cacaaacccg cggccccctt ctagacaatg aacaggtcga tatggatatc
    22921 gcgaccttta ccatcacgga cgaacgcaaa aaactctaca actttaccag tccctactac
    22981 acagacgctt ctggattttt ggtcaataaa tctgccaaaa tcaaaaagat tgaggaccta
    23041 aacggcaaaa ccatcggagt cgcccaaggt tctatcaccc aacgcctgat tactgaactg
    23101 ggtaaaaaga aaggtctgaa gtttaaattc gtcgaacttg gttcctaccc agaattgatt
    23161 acttccctgc acgctcatcg tatcgatacc ttttccgttg accgctctat tctatctggc
    23221 tacactagta aacggacagc actactagat gatagtttca agccatctga ctacggtatt
    23281 gttaccaaga aatcaaatac agagctcaac gactatcttg ataacttggt tactaaatgg
    23341 agcaaggatg gtagtttgca gaaactttat gaccgttaca agctcaaatc atctagccat
    23401 actgcagatt aaggaggaca ccccatgaca gatttatcat cttggacagc ctattttcag
    23461 gattttggac aatttttcaa tggtttcctc ttcacccttg ccctagcggt tggttccttt
    23521 atcctcgcta tggttttggg catcttcttt ggtgccatgt caaccagtaa acgtccaatt
    23581 ttacgcattt tagctcgcat ctttgttgaa ttttaccaaa atactcccct cttggtgcag
    23641 tttgttattg ttttttatgg cctacctctt atcagtgacc atatcatcat gattccaatt
    23701 tattggacag ccgttctctg cgtgggactc tatcacggcg cttatatcgc tgaggttatt
    23761 cgttcaggga ttcagtctat tcctagtggt cagatggagg ccgccttgtc gcaaggtttt
    23821 acctatatca gtgccatgcg cttgattatc ttgcctcagg ccttccgcat cattctccct
    23881 ccattgacca accaaattgt taacctcatc aagaacacct ctactgtagc tatcatctct
    23941 ggagtagact tgatgtttgt gactaagtct tggtcggctc tcaacggaaa ctatatacca
    24001 gcctttttag gtgctgctct tctctacttt gctctatgct tccctgttgc ccagtttggt
    24061 cgcaagatgg agcaagccaa caaaaaagcc tattcacttt aggaggttac tatggaatct
    24121 attttagaag ttttaacccc agataaccta gtctttatct ttaaaggatt tggcttgacc
    24181 ctctatattt ctctgattgc catcatcctc tctactatca tcggtacggt gctagctgtc
    24241 acgagaaatg gcaaaaatcc tgtcttacgc attatttcca gtatttatat cgagtttgtg
    24301 cgcaacgttc ccaaccttct ctggattttt actatctttt tggtgttcaa aatgaaatcc
    24361 acaccagcag gtattacagc ctttactctc tttacatcag cagccttggc tgagattatt
    24421 cgaggcggtc tcaatgccgt agacaaggga cagtacgaag caggaatgtc acaaggcttc
    24481 acctcagccc aaatcctcta ctacatcatt ctcccacaag ccatccgcaa aatgctacca
    24541 gccatcattt ctcagtttgt taccgtgatt aaggatacca gtctcctcta ctctgttatc
    24601 gccctacaag aactctttgg agccagccaa attctcatgg gccgttattt cgaaccagag
    24661 caggtcttca gtctttacat cctgattgcc ctcatctact tcagctttaa cctagcaatt
    24721 tctagcctgt ctcatatgct agccaaacgt tggcaacaag ctgcagaata acactcttcg
    24781 aaaatctctt caaaccacgt cagctctatc tgcaacctca aaacagtgtt ttgagcaact
    24841 tgcggctagc ttcctagttt gctctttgat tttcattgag tataa
//
LOCUS       DAVKGC010000019        24671 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKGC010000019 DAVKGC010000000
VERSION     DAVKGC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.019"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(115..1172)
                     /locus_tag="VW049_000625"
                     /pseudo
     CDS             complement(115..1172)
                     /locus_tag="VW049_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681261.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1743..3692
                     /gene="mutL"
                     /locus_tag="VW049_000626"
     CDS             1743..3692
                     /gene="mutL"
                     /locus_tag="VW049_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9431791.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGYGISHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGYGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(4017..4484)
                     /locus_tag="VW049_000627"
     CDS             complement(4017..4484)
                     /locus_tag="VW049_000627"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9431792.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4485..5690)
                     /locus_tag="VW049_000628"
     CDS             complement(4485..5690)
                     /locus_tag="VW049_000628"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9431793.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5710..6345)
                     /gene="ribE"
                     /locus_tag="VW049_000629"
     CDS             complement(5710..6345)
                     /gene="ribE"
                     /locus_tag="VW049_000629"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9431794.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(6330..7430)
                     /gene="ribD"
                     /locus_tag="VW049_000630"
     CDS             complement(6330..7430)
                     /gene="ribD"
                     /locus_tag="VW049_000630"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9431795.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7565..7679)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7836..8429
                     /gene="ruvA"
                     /locus_tag="VW049_000631"
     CDS             7836..8429
                     /gene="ruvA"
                     /locus_tag="VW049_000631"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9431796.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8439..9002
                     /locus_tag="VW049_000632"
     CDS             8439..9002
                     /locus_tag="VW049_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9431797.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8999..9676
                     /locus_tag="VW049_000633"
     CDS             8999..9676
                     /locus_tag="VW049_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9431798.1"
                     /translation="MMSNKNKEILIFAILYTVLFMFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9831..10862)
                     /locus_tag="VW049_000634"
     CDS             complement(9831..10862)
                     /locus_tag="VW049_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9431799.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(11117..11800)
                     /locus_tag="VW049_000635"
     CDS             complement(11117..11800)
                     /locus_tag="VW049_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9431800.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11812..12756)
                     /locus_tag="VW049_000636"
     CDS             complement(11812..12756)
                     /locus_tag="VW049_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9431801.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12888..15719
                     /gene="uvrA"
                     /locus_tag="VW049_000637"
     CDS             12888..15719
                     /gene="uvrA"
                     /locus_tag="VW049_000637"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9431802.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15712..16773
                     /locus_tag="VW049_000638"
     CDS             15712..16773
                     /locus_tag="VW049_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9431803.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16905..17303
                     /gene="spx"
                     /locus_tag="VW049_000639"
     CDS             16905..17303
                     /gene="spx"
                     /locus_tag="VW049_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9431804.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            17369..17938
                     /locus_tag="VW049_000640"
     CDS             17369..17938
                     /locus_tag="VW049_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9431805.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            18025..18291
                     /locus_tag="VW049_000641"
     CDS             18025..18291
                     /locus_tag="VW049_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9431806.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            18295..18714
                     /gene="ruvX"
                     /locus_tag="VW049_000642"
     CDS             18295..18714
                     /gene="ruvX"
                     /locus_tag="VW049_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9431807.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGKFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18730..19035
                     /locus_tag="VW049_000643"
     CDS             18730..19035
                     /locus_tag="VW049_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9431808.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            19393..20643
                     /locus_tag="VW049_000644"
     CDS             19393..20643
                     /locus_tag="VW049_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9431809.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLICQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLDIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20640..21158
                     /locus_tag="VW049_000645"
     CDS             20640..21158
                     /locus_tag="VW049_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9431810.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKSIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDGTYTGQDDG
                     DRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNGNQIVVDDM
                     DRDPSDQIVLTK"
     gene            21304..22836
                     /gene="cls"
                     /locus_tag="VW049_000646"
     CDS             21304..22836
                     /gene="cls"
                     /locus_tag="VW049_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9431811.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            23094..24632
                     /locus_tag="VW049_000647"
     CDS             23094..24632
                     /locus_tag="VW049_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9431812.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
BASE COUNT         7193 a         4737 c         5269 g         7472 t
ORIGIN      
        1 gaagattaaa atacagtaaa aaaatcaact aaaatagtcc caaagtaaag aaatacttga
       61 acctatttta gctggttttc ttttttttca cctctaattg aggattattc tattttaggg
      121 tatactcccc catgttttca accctaatcg ttggatttgt tcgaaacgac agatattata
      181 caacaaattt gtcaaaatca cattcgtttt tgtacgcgct aatccaattc ctctaaaatg
      241 acttcctttc atcgtatttt taataacacc aaaaacatgt tccacacgac agcggatacg
      301 agaaatagac tgattgatac gcttatccaa atcagtcaag gttttatttc gaaaagcact
      361 atcatcaaag atcggctcct tactctcaca caactcttcc aagacgcgag aatcgtgaat
      421 attagcagaa gtaatctcaa atttcttgat tagttttgat ttttggtcga tactaatact
      481 caatgaaaat caaagagcaa actaggaagc tagacgcaag ttgctcaaaa cactgttttg
      541 aggttgcaga tagagctgac gtggttttaa tttgattttc gaagagtata atatgatttg
      601 tataaccaaa gtaagttggt tctccctagt ccatcctgta tccctatctt tttgagaagg
      661 cttcgctgta ttccaatctg tcggaatctt tccttttttg atttgttgat tctcttctcg
      721 tgtattgcgt tgtttagggg attctacaaa tggggcatct actatctgtc cctgatgagc
      781 taataagcct ttttctgcta gtgttagata aaattactca gataattctt gttctttctc
      841 tgatttggtc aagcgttctc tatacaacca gatggttttt gcatcatgta ctttacattc
      901 atcacaacct gtggaatgac gaaatgacag acgatgaaag agttgatatt ccatcgcatc
      961 atctgataaa ttatggagat gttggatcaa gagcacctta aatatcatga aaaaatcatg
     1021 cggagggagt ccgccttttg atggtttcta aaaatctgct caagaatagg tctaatttgc
     1081 tcccaatcca ttgccttatc taagccttcc aatggattgc ccattgaact caattttgat
     1141 agtgtttgtt catcatctat aaaaaattcc atcgcctaac ctagcttacc tctatctcta
     1201 gtatctcatg ttttacgatt tttttgggtt tttagaggtg cccttaaatt cgttcattaa
     1261 tgttacgaga ctggccgaaa attagtctct aaataaaacc acacagtgat ttcagtcttg
     1321 aatccaatca aactgaaaga agcactatgt ggtttttgaa tagtgggcta gattcttgat
     1381 caatttggtg agattcatgg taatcgccca ataacgaggt atattgaaaa atctacagac
     1441 tagagaactc actgatagtt cctaatctgg agatttctta tttgcacttt tcttgtacaa
     1501 ctttagtcca tggtaaataa gcctctaaaa catctttatt tacgagagtc tcctcgccta
     1561 gctttctagt ttgttcttta atttttattg agtataccac tattttactc cctctggcaa
     1621 gggactttgt ctatgtggag ggattgggct cctatgtggt ggagcttttc tgttctttct
     1681 gaaatatggt ataatagcac taatcaattt ctaggaaaat agatacagaa aggggctgaa
     1741 agatgtctca tattattgaa ttgccagaga tgctggcaaa ccaaatcgcg gctggagagg
     1801 tcattgaacg tcctgccagt gtggtcaaag agttggtaga aaatgccatt gacgcgggct
     1861 ctagtcagat tatcattgag attgaggaag ctggtctcaa gaaggttcaa atcacagata
     1921 acggttatgg aatttcccac gatgaggtgg agttggccct gcgtcgccat gcgaccagta
     1981 agataaaaaa tcaagcagat ctctttcgga ttcggacgct tggttttcgt ggtgaagcct
     2041 tgccttctat tgcgtctgtt agtgtcttga ctctgttaac ggcggtggat ggtgctagtc
     2101 atggaaccaa gttagtcgcg cgtgggggtg aagttgagga agtcatccca gcgactagtc
     2161 ctgtgggaac caaggtttgt gtggaggatc tctttttcaa cacgcctgcc cgtctcaagt
     2221 atatgaagag ccagcaagcg gagttgtctc atatcattga tattgtcaac cgtctgggct
     2281 tggcccatcc tgagatttct ttcagcttga ttagtgatgg taaggaaatg acgcggacag
     2341 ctgggactgg tcaactgcgc caagctattg cagggattta cggtttggtc agtgccaaga
     2401 agatgattga aattgaaaat tctgacctag atttcgaaat ttcaggtttt gtgtccttgc
     2461 ctgagttgac tcgggctaac cgcaattata tcagcctttt catcaatggt cgttatatca
     2521 agaatttcct actcaatcgt gctattttag atggttacgg aagtaagctc atggtaggcc
     2581 gttttccact ggcagtcatt cacatccata tcgaccccta tctagcggat gtcaatgtgc
     2641 atccaaccaa gcaagaggtg cgaatttcta aggaaaaaga actgatgacg ctggtctcag
     2701 aagctattgc caacagtctc aaggagcaaa ccttgatacc agatgccttg gaaaatcttg
     2761 ccaaatcgac cgtgcgcaat cgtgagaagg tggagcagac cattctccca ctcaaagaaa
     2821 atacgctcta ctatgagaaa actgagccgt caagacctag tcaaactgaa gtagctgatt
     2881 atcaggtaga attgactgat gaagggcagg atttgaccct gtttgccaag gaaaccttgg
     2941 accgattgac caagccagca aaactgcatt ttgcagagag aaagcctgct aactacgacc
     3001 agctagacca tccagagtta gatcttgcta gcatcgataa ggcttatgac aaactggagc
     3061 gagaagaagc atccagcttc ccagagttgg agtttttcgg acaaatgcac gggacttatc
     3121 tctttgccca agggcgagat ggactttaca tcatagatca gcacgctgct caggaacggg
     3181 tcaagtacga ggagtaccgt gaaagcattg gcaatgttga ccaaagccag cagcaactcc
     3241 tagtgcccta tatctttgaa tttcctgcgg atgatgccct gcgtctcaag gaaagaatgc
     3301 ctctcttaga ggaagtgggc gtctttctag cagagtacgg agaaaatcaa tttattctac
     3361 gtgaacatcc tatttggatg gcagaagaag agattgaatc aggcatctat gagatgtgcg
     3421 acatgctcct tttgaccaag gaagtttcta tcaagaaata ccgagcagag ctggctatca
     3481 tgatgtcttg caagcgatct atcaaggcca atcatcgtat tgatgatcat tcagctagac
     3541 aactcctcta tcagctttct caatgtgaca atccctataa ctgtcctcac ggacgtcctg
     3601 ttttggtgca ttttaccaag tcggatatgg aaaagatgtt ccgacgtatt caggaaaatc
     3661 acaccagtct ccgtgagttg gggaaatatt aaaagtataa aaaagtctgg gaaaaatttt
     3721 caaaatcaaa aaaacgcata aaatcaggtg ttcaaaaacc ttgattttat gcgttttatc
     3781 atggaaatag ttacttcatt ttttcctaat tcttttcgaa actcttttta aacgacgtca
     3841 gttttatcag taatctcaaa acagtgtttt gagctaattt tgccagtttt gtctgtaaca
     3901 tcgaagttgt gtttgaccac tctgcgactg gtttcctagt ttgctctatg attttcacag
     3961 agtattaaat tgcgattttg ccaagtttct ttattcgtct aaaagtagag tctgttctat
     4021 gcgtctaatg tacgaattag gttgaccatt tcaatagctc cttgtgcaca ctcagaaccc
     4081 ttatttcctg ctttagtacc agctcgttct atggcttgtt caattgtatc tgtcgttagc
     4141 acaccaaaca taacaggaat ttcgctattt aaactgattt gggcgattcc cttagatacc
     4201 tcgctacata cataatcata atgacttgta tttcctctaa tgacagctcc caagcagata
     4261 attgcatcat attttttact ttttgccatt tttgatgcaa tcagtggtat ttcaaaagct
     4321 cctggaaccc aggctacctc gatatctttc tcgtttacat tctctctttt gagattatct
     4381 agtgctccag ataataattt tgaagttata aattcattaa atctcgctac aacaatacct
     4441 attttaatat tgtttgctac taaattacct tcataagtgt tcatttattt ttcctccata
     4501 tttaaaatgt gacccattcg atttttcttt gtttctaaat aaaaactatc gtaaggattg
     4561 gcttctattt cgattgatat tctactggaa atggtaattc catatttttc taactgttca
     4621 accttgtcag gattatttgt cagtaaatga agtgactgaa gtcccagatc tttaagcatt
     4681 tgtgctccaa tatgatattc tcttaaatca ccttcaaagc ctaatgcaag attggcatca
     4741 agcgtatcca tgccttgatc ttgtaaatga taggctttta atttattgat aagtccaatt
     4801 cctcgtccct cctgtcgcaa gtaaagtaag acacccgaac cattctcaac aatcattttc
     4861 atagctttat cgaattgctg tccacaatcg caacgtaaag agcctaaaac atctcctgtt
     4921 aaacattcgg agtggacccg acataataca ttggcttcat cctctatgtt tcccataata
     4981 agagcaagat gatgttcccc atttagttta tctatatagc taattgcttt gaaattaccg
     5041 tatctagtag gcatattgac agttgaaact cgttctacca gctgatcata tacttttcta
     5101 tattcttgta attctttgat ggtaattagt ggaatgttgt gtttcttcga aaactgaatt
     5161 aaatcatctg ttctcatcat tttgccatca tgattcatta tttcacaaca taggccacac
     5221 tcttttagtc cagctaattt taataaatca acagttgctt ctgtgtgtcc atttctttct
     5281 agaacaccac ctttttttgc aattaaagga aacatgtgtc ctggcctgcg aaaatcagag
     5341 ggtgttatat cttcagctac acacatacgt gcggtcagtc ctctttcctc ggcagaaata
     5401 cctgtggtcg tttctttata atcaattgaa actgtaaaag cagtcttatg attatctgta
     5461 ttgttttcaa ccataggtga aagcattaat tgattagcta aactttcgct cataggcata
     5521 caaattaatc ctttggcata agtagccata aaattaacat tttctgttgt agctgcttgt
     5581 gcagaacaaa ttaagtctcc ttcattttct ctatccttgt cgtctataac aagaacaagt
     5641 cgtcccttct gcaatgcttc taatgcttct tgtatttttc gatattccat tgactgatta
     5701 tcctttctgc taaaatccat tttgatataa tagttcctta gatatttctg attttggaga
     5761 gttatccatc agtttttgca catatttacc taagatatca ttttcaagat ttactgtact
     5821 cccgacttgt ttactcttaa gaatggtttg ttccaaggta tgagggataa cagatactga
     5881 aaagtttact ttggagactt tagcgacagt cagactaatg ccgtcaattg taatagatcc
     5941 tttttcaact attaaatcta aaatttcttt ttgtgtgttg atttgatacc atacagcatt
     6001 atcatctttt tttattgacg agatttttcc tgtaccatca atgtgtcctg taacgacgtg
     6061 acccccaagt cgaccgttga cagataaggc tctttctaga ttcacctcac ttccatgttt
     6121 taatagagta agagctgttc gactccatgt ttcattcatt acatcaactg taaaggattg
     6181 atggttgaaa tgagtaactg taagacagat accatttact gctatactat cgcctaaatg
     6241 gatatccgtt aatatttttg aggctttaat tgatagttta caattacgag agtctttctg
     6301 tattctttca acttttccga tttcttcaat tattcctgtg aacatggata aatcacttca
     6361 ctttctatga gatagtcatt tcctatttga gaaaaagcat aaggtttcaa tctaatagcg
     6421 tcatttggca aagaaatgcc ttcacctccg acaggaaact tggcactgcc tccaaaaatt
     6481 tttggtgcaa tatatatttt cagctcatca acaatttgtt gttccaaagc actccaattc
     6541 attagactgc ccccttctag aactaggcta tcaatctgca tgtttcctag atgttgcatt
     6601 aaactcgata agtctatatg attgcctttt ttctttatgg aaagtatttc acagccatga
     6661 ttttgatata gcttcatttt atttttgtct tcagaggaag tggcaatgta agttttaata
     6721 tcatttgctg tttttacgat tttagaggta agaggagttc gtaaatgtgt atcgcatatg
     6781 atacggatag gatttttccc ttcctccaat ctacatgtca gcaaaggatc gtcttgaata
     6841 acagtattga ctcccaccat aattgcacta acatagtgtc gtaactgatg cacatgcttt
     6901 cttgcttctt cttcagtaat ccatttggat tgatttgttt tagtggctat ttttccatcc
     6961 attgacattg catatttcat aaaaacatag ggtacatgct gggtaatata ctttctaaaa
     7021 ctttttatta agttaagaca ctcattttct aaaattccaa cagtaacttg aagattattt
     7081 tcctcaagta tctttactcc ttttccagat acaataggat tacagtctag gcttccaatg
     7141 actactcttg taataccact atcgattata gcatctatac agggaggtgt tttcccgaag
     7201 tgacaacagg gttcaagtgt tacataaagc gtcgctccga caggggattc tctacagttt
     7261 ttaagagcat ttctctcagc atgtgggcca ccaaaaaact catgataacc ttgtccgatg
     7321 atgtgattat cttttacaat aactgcgccg accataggat tgggattgac gtaaccagcc
     7381 cctttttgtg ccagttttat tgctaatttc atatattttg aatcgctcat ctcactacct
     7441 ccaaaaaaaa tataccttga ataggggact actcaaggca tacaaaagaa aacttatgcg
     7501 attaacaaaa atactctgaa atgacaagta atcatttcag agcacgcaaa aagcacaaat
     7561 atacttttat cttctttcat ccagactata ctgtcggctt tggaatttca ccaaatcatg
     7621 cctttcggct cgtgggctat accaccggta gggaattaca ccctgccctg aagatagtta
     7681 ttcaattaca gatgattata gtacttaatt ttgaatatgt caacagataa ataccgattg
     7741 tttttgatat actgtatttg tgataatcga ttctcgctcc tcggataaag aaaatatgat
     7801 atactagata aacgaaataa gagagaagga atactatgta cgcatattta aaaggaatca
     7861 ttaccaaaat tactgccaaa tacattgttc ttgaaaccaa tggtattggt tatatcctgc
     7921 atgtggccaa tccttatgcc tattcaggtc aggttaatca ggaggctcag atttatgtgc
     7981 atcaggttgt gcgtgaggac gcccatttgc tttatggatt tcgctcagag gatgagaaaa
     8041 agctctttct tagtctgatt tcggtctctg ggattggtcc tgtatcagct cttgctatta
     8101 tcgctgctga tgacaatgct ggcttggttc aagccattga aaccaagaac atcacctact
     8161 tgaccaagtt ccctaaaatt ggcaagaaaa cagcccagca gatggtgctg gacttggaag
     8221 gcaaggtagt agttgcagga gatggccttc ctgccaaggt cgcagtgcaa gcaagtgctg
     8281 aaaaccaaga attggaagaa gctatggaag ccatgttggc tctgggctac aaggcaacag
     8341 agctcaagaa aatcaagaaa ttctttgaag gaacgacaga tacagctgag aactatatca
     8401 agtcggccct taaaatgttg gtcaaatagg agcagagaat gacaaaacgt tgttcgtggg
     8461 tcaagatgac caacccgctc tacatcgcct atcatgatga ggagtggggt cagcccctcc
     8521 atgatgacca agcattgttt gagttgttgt gtatggaaac ctatcaggca ggcctgtctt
     8581 gggaaacggt actcaacaaa cgccaagctt tccgagaagc ctttcatagc tatcaaattc
     8641 actcagtcgc agagatgact gagactgaat tggaagccat gctggagaat ccagctatca
     8701 ttcgaaatag agccaagctt tttgctacac gcgctaacgc ccaagccttt ctacagttac
     8761 aggcagagta cggctctttt gatgcctatc tttggtcttt tgttgagggg aaaactgtcg
     8821 ttaacgatgt tcctgattat cgccaagccc cagctaaaac acccttatct gagaaattag
     8881 ccaaagatct caaaaaacga ggcttcaagt tcacaggccc agtcgccgta ttgtcttttc
     8941 tacaggctgc agggctagtt gatgaccacg agaatgattg tgagtggaaa ggtcttaaat
     9001 gatgtctaac aaaaataagg aaattctgat ttttgcgatt ctctatacag tcctctttat
     9061 gtttgatggc gttaaattgc tggcttcttt aatgccatct gccattgcaa attatcttgt
     9121 ttatgtagtt ttagctctat atggctcctt cttgttcaag gatagattga tccaacaatg
     9181 gaaggagatt agaaagacta aaagaaaatt cttctttggc gtcttaacag gatggctctt
     9241 tctcattctg atgactgttg tctttgaatt tgtatcagag atgttgaagc agtttgtggg
     9301 actagatgga caaggtctaa atcagtctaa tattcaaagt acctttcaag aacaaccact
     9361 actgatagct gtttttgctt gtgtcattgg acctctggta gaagaattat ttttccgtca
     9421 ggtcttattg cattacttgc aggaacggtt gccaggttta ctaagcatta ttctggtagg
     9481 acttgttttt gctttgactc atatgcacag tttggctcta tcagagtgga ttggtgcagt
     9541 tggttactta ggtggaggcc ttgccttttc tattatttat gtgaaagaaa aagagaatat
     9601 ctactatccc ctacttgttc acatgttaag caacagcctc tccttaatca ttttagctat
     9661 cagtatagta aaatgaaata agaacaggac aaatcgatca ggacagtcaa atcgatttct
     9721 aacaatgttt tagaagcaga ggtgtactat tctagtttca atttactgta atatgtgatg
     9781 aaaatgccag taatgatacc gagaaaaaag ctgagaaact tttcccagct ttatttgtta
     9841 tagtcaaagc gaatgacttg ttcctgtgca tctacatgag catggacccc aaagggtaca
     9901 attgctcttg gagttgcgtg gccgacattc agattataga caatcgggat attgctgtca
     9961 atgatatcca atagtgcctc tttatagtcg tcatggaaag tttcatccat aggttttccg
    10021 accaagagtc cattgatgac cgcgaatatg ccagtgtcct ttaaagttag caacatcttt
    10081 ttgaagtctt ctggcttagg cttttcttcg cttgtttcga gcaagaggat tttcccttcc
    10141 cagtctgaca agtcagggaa aagtttgtat ttttggcaga gctccgtgct atctgcgtat
    10201 cgagagttgt caaagatatc gtagagggat tcgaggcaac caccgaggat tttcccctcg
    10261 aattgggccc ttccttgcaa caagtcaaaa cctgtatttg tatgactgac acgaggtgtt
    10321 cccagggccg tgggactaaa atcagttcgt tcctcatacc aaacgtcact agggcggatt
    10381 tctgaaattc ttcccgtctc aatcaattct ttaaagtagt gaaggctata ggctagcatt
    10441 tctttgtcta attcacaaat gtctgctaaa aaggattgac cataaaaagt cttgattcct
    10501 aatttatgca acatgaggtg gttcatggtt gtatccgaga agccaagaaa aattttttgt
    10561 ttgataacct tttggagttg gtcattttca aaaagataag gtagcaagcg ataggtatcg
    10621 tctccaccga tggcacatag gatcatgtcg atgctatcat cagaaaaggc atgaatcaaa
    10681 tcctctgcac gagcttcagg atggtccttg ataaagtcta atccttttaa cgaatggggc
    10741 aaaaagatgg gattgagtcc cagatccttg agacgttgga tacccaagtc cacttcgtgt
    10801 ttgacaaaat cctctccgat aatgccacta gataaactaa caataccaat agtagaaacc
    10861 atatctcatc ctcctagaaa tagattgagc ctattttatc acaaaatata agagaaagct
    10921 atgtgggatt tcagaagaat acttttaata ctcttcgaaa atctcttcaa atcacgtcag
    10981 ttttatttgc aacctcaaag ctgtgctttg agcaacctgc agctagcttc ctagtttgct
    11041 ctttgatttt cattgagtat aactcaaaaa agtaagtgaa aaagcaaccg cacctgcagt
    11101 tgcttttatc gttttcttat tcttgatagc gtgttggtcc tgcacttgca ctacggatat
    11161 tcaagcgttt cttgagatag aagcgaaggg ctaggagggc tgctccaata atagctagtg
    11221 gcaatggatc cagtactggg ttaaggctag ctggtaggaa gcttgttgca aagaagacaa
    11281 gcaaccaaag gaacatagaa gctaggataa ctagtacaga tttccagaaa ggtggacgtt
    11341 gactgcgatc catatctggt ccatagtatt ggtaaacaaa gtagtacatc aagtagaagg
    11401 caaatccacc aaccagtcca actaatagaa gagtaatcaa tccatagccg aaagcttgat
    11461 ctgccgcaaa gaaggttgtg agggcgctga caagggcaaa gaggctagtg atgaaaagag
    11521 ctgagtccat aatcatcagt tttgggtcat cattttcttt tggatgctct ttttcgtact
    11581 gctctttgac agtgaagcta tgagcccaat gagttggtgc gccgtatagg gaacgggcag
    11641 ttgtaccttt agattgctcc tcaaggattt ggggaatgac ttcctcaaaa atagtctgga
    11701 tttcagcgtc tgttttccca tctttgatga attgttgggt agcaatgtgg acaaactctt
    11761 ggtttttctt agttaatttt tgtagatcaa tttgagacat aggaactcct cttagaacca
    11821 ttttttatgg atgagataga gagtgagcga gacactcata gcaaaggcga taaagacgat
    11881 taaccagaag gcatttggct ctccgtttag gggaatttca ttatccttaa agttcatccc
    11941 gtaggcagaa aagaccatgg ttgggatgga catgacgatg gtcacaaggg ccaaggtttt
    12001 catgatgttg ttctggttgt tagaaatgat agaggcaaag gtctctgtca tagaatgcaa
    12061 gacatttcca taaatatctg ccatctcgat ggcctgttgg gtttcaatca gggtgtcttc
    12121 aagcaggtct tcgtcctcaa ggtatttctt gatattgctg gttgaactgg tcaatttctt
    12181 aatcacgcgc tcatttgttt tgagggaggc cttgaaatag acgatagttt tttccaattc
    12241 catgagctca atcaattctt cattacgagt tgattgatgc agttgacttt cgatttgttc
    12301 actcttgcgg tcgattgaac gaagggctgt taggtaaagc tctgcattgc gataaagaat
    12361 ttgaaagata aaacgtgaac gcatgaaggt atagaaatta cgcaatcgac ggttgataaa
    12421 gacatcaagg acaggtagtg gttccaaaca cgtagtgata atggtttcct cagtgatgat
    12481 aataccaagc gggatggtta cgtagtaggt gcggttattt ctttcctccg tgaccggcac
    12541 gtctacgata atcagggtat actcgtcttc aatggtaata cgagacattt cttccgcatc
    12601 gagcggtgct cgaaggtcgg caatatcaat atcgaaggtg ttggcgattt cgagtgattc
    12661 attttgagtc ggattgacga gattgatcca agtacccggt tcaagcgtat cgatctcttt
    12721 aaattcagtt gttgtagaga gaaaaacttg tttcatatcc cttatccttt ctcatttttc
    12781 agattttttc acactgtact attatactac aaaatcggcc ttttgggtaa tagaatctca
    12841 ctttttttgg tataatggta agcaataatg gactagaaag aacaaagatg caagataaaa
    12901 ttgtcattca tggggcgcgt gcccataatt taaaaaatat tgatgtggag attccgcgag
    12961 acaagttggt tgtcgtgact ggcttgtcag gttcagggaa gtccagtctg gcttttgata
    13021 ctctctatgc ggagggacaa cgtcgctatg tagagagttt gtctgcctac gctcgtcagt
    13081 tcttgggaaa tatggaaaaa ccagatgtag atgccattga tggtctcagc ccagctattt
    13141 ccatcgacca gaaaacgact agtaaaaatc ctcgctcgac ggtgggaacg acgactgaaa
    13201 tcaatgacta tctgcgtctc ctctacgcac gtgtggggac gccttactgt atcaacggac
    13261 atggagctat caatgcttct tctgtggagc aaatcgttga taaggttttg gagttacctg
    13321 aacgccagcg cttgcagatc ttggctcctg tcatccgcaa gaaaaaaggc caacataaga
    13381 gtgttatcga gaaggttcag aaagacgggt atgttcgtgt tcgtgtggat ggggaagtct
    13441 atgatgtgac cgaagtgcca gagttgtcta agagcaagca acacaatatc gatgtcgtgg
    13501 ttgaccgtat tgttatcaag gagggcattc gtagccgtct ctttgattcc attgaagctg
    13561 cccttcgtat cgcagaaggt tatgtgatta tcgacaccat ggacgactct gagttgttgt
    13621 tctctgagca ttatgcctgt ccagtttgtg gatttactgt cccagagtta gaacctcgtc
    13681 tcttctcttt caatgctccc tttggctctt gtagtgagtg tgacggcttg gggattaagc
    13741 tggaggtgga tactgatttg gtagtgccag atgccagcaa aaccttacat gagggagcgc
    13801 tggcaccttg gaatcctatc tcatccaact actatccaaa catgttagag caggccatga
    13861 aagtctttgg agtggctatg gataagcctt ttgaggacct gtcagaagaa gataagaact
    13921 tgattctcta tgggtcagat ggtaaggaat tccatttcca ctatgagaat gaatttggtg
    13981 gcgtgcgcga tatcgacatt ccgtttgagg gagttatcaa taatatcaaa cgtcgttacc
    14041 atgaaaccaa tagcgattac actcgcactc agatgcgtct ctacatgaat gagctgacct
    14101 gcggaacctg tcaaggctat cgtctcaatg accaggcctt gtctgtccgt gtgggcggcc
    14161 agcaagggcc acatattgga gaaatctcag acctgtctat cgctgaccac ttggacttgg
    14221 tgagccagtt aactttgtct gaaaatgaag ccatcattgc tcgtcccatt ctcaaggaaa
    14281 tcaaggatcg tttgaccttc cttaataatg tgggtcttaa ctatctaacc ctgtcacgtt
    14341 cagcaggaac cctttcaggt ggggaaagtc agcgcattcg tttggcaacc cagattggtt
    14401 ccaacctatc aggcgtcctc tatatcctag acgagccgtc aatcggtctt caccagaggg
    14461 acaatgaccg cctgattgcc agtctgaaaa agatgcgtga cttgggcaat actcttatcg
    14521 tggtggaaca cgacgaagat accatgcgtg aagctgatta tctgattgac gttggtcctg
    14581 gtgccggtgt ttttggtggt gagattgttg cagcaggtac acctaagcaa gtggctcgca
    14641 acagcaagtc tatcacaggt cagtacttgt caggcaaacg tgtcatccca gtaccagaag
    14701 agcgccgtgt cggaaatggt cgttttattg aagtgatagg agcgcgtgag aacaacttgc
    14761 aaaatgtcac ggctcgcttt ccactaggaa aatttatcgc agtgacaggt gtgtcgggtt
    14821 cagggaaatc gactctaatc aacagcattc tcaaaaaagc cattgcccag aagctcaacc
    14881 gtaattcaga caaacctggt aagtttaaga ctattacagg gattgagcat gtcgaccgtt
    14941 tgattgatat tgaccagagc cctatcggac ggacgccgag gtctaaccca gctacctata
    15001 caggagtttt tgacgatata cgtgaccttt ttgctcagac aaatgaagcc aagattcgag
    15061 gctacaagaa ggggcgcttc agtttcaacg tcaagggagg tcgctgtgaa gcttgctcag
    15121 gtgacgggat tatcaagatt gagatgcact tcttgccaga tgtttatgtg gcttgtgaag
    15181 tttgccacgg aacccgctat aacagtgaaa ccctagaagt tcactacaag gaaaagaata
    15241 tttcgcaggt cttggatatg acggtcaacg atgcggtaga atttttccaa cacattccga
    15301 aaattcaacg caaactccag accatcaagg atgtagggct aggctatgtg accttgggac
    15361 agccagctac caccctttct gggggagaag cccaacgtat gaagttagct agcgaactcc
    15421 acaaacgctc gacaggaaaa tctttctaca ttctggatga gccgacgaca gggcttcata
    15481 cagaggacat tgctcgcctg ctcaaggttt tagctcgctt tgtagacgat ggcaatacag
    15541 tcctcgtcat cgagcacaat ctggatgtta tcaagacggc agaccatatc attgacttgg
    15601 gacctgaggg cggtgtcggt ggtggaacca tcatcgtaac aggaactcca gaagaagtag
    15661 ctgccaacga agccagctat acaggacact atttgaaagg aaagttacat catgaataaa
    15721 cgcgtacaag catttctagc taaaatgcaa gaaaaagaac tagatggtat catcatcaac
    15781 aatcttaaaa acgtctatta tttgactggt ttttggggct caaacggaac agtctttatc
    15841 agccgtgacc gtcaggtctt agtgacagac tctcgctata tcatagcagc taagcaagaa
    15901 accagtggtt ttgagattgt ggctgatcgt gatgaattgg ctgtcattgc aggaattgtt
    15961 aaggacatgg gcttgactcg tatcggtttt gaagatgaga tttcagtgtc ttattaccac
    16021 cgtatgcagg cagcttttgc aggtttggac ttgcttccac aaactcagtt tgtggaaggt
    16081 cttcgtatga ttaaggatga ggcagagatt gcagcgattc gcaaggcttg ttctatctca
    16141 gaccaagctt tccgcgatgc gcttgacttt attaaaccag gaaaaactga aattgagatt
    16201 gccaacttcc ttgacttccg catgcgtgag ttgggagcat ctggcttatc ttttgatacg
    16261 atcctagcta gcggtatcaa ttcttctaaa ccccatgccc atccaatgca caaaccagtg
    16321 gagttgggag aagccattac catggacttc ggctgtctct atgaccacta tgtcagtgat
    16381 atgacccgga ctatctatct agggcatgtt agcgatgagc aggcagagat ttacaatacg
    16441 gttctaaaag ctaaccaagc cttgattgac caagctaagg caggcttagg tttccgtgac
    16501 tttgacaaaa tccctcgtga tattatcatt gaggcaggtt atggcgacta ctttactcac
    16561 ggcattggcc acggtattgg tctggatatc catgaggaac cctacttcag ccagacttct
    16621 acagaaacta ttaagacagg tatggccttg accgatgaac caggtatcta tatcgaaggc
    16681 aaatatggcg ttcgtatcga agatgatatc ctgattacag agacaggttg tgaattattg
    16741 accctagctc caaaagagtt gatagtcatt tagttgtttg tcaagaaaaa agtacgaaaa
    16801 tgacgaaaaa agtcaaaaaa aattaaaaat aggtcgcaag tcggatgttt tttatggtat
    16861 aatagactaa actgatagta acatgtagcg aaaggggtag gtacatgatt aaaatttata
    16921 cagtctcaag ttgtactagc tgtaaaaaag caaaaacctg gctcaatgcc caccagttaa
    16981 gttataaaga acaaaacctt ggtaaagaag gaattacgag agaagaatta ctggatattc
    17041 taaccaaaac agataacgga atagccagca tcgtttcgtc taaaaatcgc tatgccaaag
    17101 cccttggagt ggatattgaa gatttgagtg tcaatgaagt tctcaatctg attatggaaa
    17161 caccgagaat tttaaagagc ccaatccttg tagatgaaaa acgcctgcaa gttggctaca
    17221 aggaagacga tattcgtgcc ttcctaccac gctctgtccg taatgtagaa aatgcagaag
    17281 cacgtttgcg tgcagctcta taaacatcaa ggctgggagc acttcccagt cttattctat
    17341 tttaatttca aaaagaaaga agaaagaaat gaaaaaaata gttcttgtta gtctagcttt
    17401 cctttttgtc ctggttggtt gcggacagaa aaaagaaact ggaccagcta caaaaacaga
    17461 aaaagatacg cttcagtcgg cattgccagt tattgaaaat gctgagaaga atacagttgt
    17521 aactaagact ttggtcttgc ccaagtcaga tgatggtagc cagcagacac aaacaattac
    17581 ttacaaagac aagacttttt tgagtctaac tatccaacaa aaacgtccag tctctgatga
    17641 gttgaagact tatattgacc aacatggagt ggaggaaact caaaaagctc ttcttgaagc
    17701 ggaggagaag gataagtcta tcattgaagc tcgtaaactg gcaggtttca aacttgaaac
    17761 aaaactattg agcgcaacgg aacttcaaac aacgactagt tttgattttc aagttctgga
    17821 tgtcaagaag gcttcccagt tggaacatct gaagaatatt ggtttggaaa atcttttgaa
    17881 aaatgaacca agcaaatata tttcagatag attggcaaat ggcgcgacag aacaatagaa
    17941 aatccaaata aatagactgc ctgaattgta gaaggtaagg aattatgcta taatggtact
    18001 attctaagga aagagggtgt tagaatggga tttactgaag aaacagtacg ttttaaattg
    18061 gacgattcca ataaaaaaga aattagcgaa actttgacag atgtttatgc ttcgttgaac
    18121 gataagggtt acaacccaat taaccaaatc gtaggttacg tattgagtgg agaccctgcc
    18181 tacgttcctc gttataataa tgcacgaaat caaatccgta agtatgagcg tgatgaaatc
    18241 gttgaggaat tggttcgcta ctacctcaaa gggcaaggag tcgatctata acctatgaga
    18301 attatgggat tggacgtcgg ttcaaaaacg gtaggggtgg cgattagcga tccgcttggt
    18361 tttacagctc aagggcttga aatcatccag ataaatgaag aacaaggcaa atttggtttt
    18421 gaccgcgtta aggaattggt tgatacttac aaggtggaac gatttgtagt gggcttgcct
    18481 aaaaacatga acaatacaag tggaccgcgc gtagaagcta gtcaagccta cggagcaaag
    18541 ctagaagagt tttttggttt accagtagac tatcaggatg aacgcttgac aacagtggct
    18601 gctgagcgca tgttgattga acaagcagat atcagtcgca ataagcgcaa gaaagtcatt
    18661 gataagttag cagctcagct gattttacaa aattatttag atagaaaatt ttaatataaa
    18721 ggagaggcta tgtcacacga tcataaccac gaccacgaag aacgtgaact aatcacacta
    18781 gtagatgagc aaggaaatga aaccttgttt gaaatccttt tgacaattga tggaaaagaa
    18841 gaatttggta aaaactatgt tcttctagta ccagttaacg cagaagaaga cgaagacgga
    18901 caagttgaaa tccaagctta ctcattcatt gaaaatgaag atggaacaga aggcgaattg
    18961 caaccaatcc cagaagactc agaagacgaa tggaacatga ttgaagaagt cttcaacagc
    19021 tttatggagg agtgatacag tctgggagac tgtatcagcc caactcccag aaatggggaa
    19081 gagttggaac gtccagtgga tgtttttagc ccagttcctt taaataagag agaactggta
    19141 ggttcggggg gacgttttta gccctgcgct agaaattaga aacgcaggta gtctgagaaa
    19201 ctgtatcagc ccaactccca gaaatgggga agggttggaa cgtccagtgg atgttttact
    19261 ccacgttaat attcatagtt gctagtgatt agctaaccag gtaagaggtc gggacgaaaa
    19321 tcctggcctt gtttttatta gtaaccatac tttaatgcga taattgattg gacttttgtc
    19381 aaggagatgt gtatgtttga agtagaagaa tggctccata gtcggattgg tttaaatttt
    19441 cgatcaggtt tgggccgaat gcagcaagcg gtggatttgt taggaaatcc tgagcagtct
    19501 taccctatta tccacgtaac agggactaat gggaaaggat ctaccattgc ttttatgagg
    19561 gaattattta tggggcatgg caaaaaagtt gcgaccttta cctcccctca tatcgtctct
    19621 atcaatgacc gaatctgcat taatgggcaa cctatagcag acgcagactt tatccgtttg
    19681 gctgatcagg tcaaggagat ggagaaaacg cttctgcaaa ctcctgacca gttgtccttt
    19741 tttgaattgc tgaccttggt tgcttttctt tattttaggg agcaagaggt ggatttggtt
    19801 ttattagaag tgggaattgg tggcttactt gacacgacca atgtggtaac tggagagctt
    19861 gctgtcatca cctccattgg gcttgatcat caagaaacct tgggtgatag tctagaagca
    19921 attgcagagc agaaagctgg tattttcaag gctggtaaga aggcagtgat tgcgaaattg
    19981 cctccagaag ctaggcttat ctgtcagaaa aaagccgaat ctttagctgt taacctttat
    20041 caggcaggtc aagatttttt aatgctgaat ggtgattttt caagctcttt actaaatctt
    20101 tcacagctgg acataggctt agaaggagtc tatcagcagg agaatgcagc cttggcgttg
    20161 caaacttttc ttctttttat gggagaagga aaggaagctg ttgatgaaca ggctgtaaga
    20221 aaggccttgg aacagaccca ttgggctggt cgcttggagc gtattcgccc acagatttac
    20281 ttggatggtg ctcataacct ccctgccttg actcgcttgg ttgagtttat caaagaaaaa
    20341 gagcaggaag gttatcgacc tcaaatcctc tttggatcct tgaaacgtaa ggattatcaa
    20401 gggatgttgg gttatctgac tgaaaattta cctcaagtgg aactcaagat gactggcttt
    20461 gactatcaag ggtctttgga cgagacagat gtaacaggtt accatgtaat tccctcttac
    20521 cgagaattta tcagcagttt cgaagaaagg gcagacgctc aagacttgct gttcgtaaca
    20581 gggtctctct attttatctc agaagtacgg ggctacctgc tggaccgtga gcagataaat
    20641 tgaccccctt ttttgaactt gttatactag gggaattgct ggttagagaa aatttctcta
    20701 aattggtagc agaaaggaaa tccatcatga aattaaaaag attcacactt tctcttgctt
    20761 ctctagcaag ttttagtctc ttagtagctt gttcacaaag agctcaacag gttcaacagc
    20821 ctgctcaaca gaataccaat actgcaaatg cagggggtaa ccaaaatcaa gcggctccag
    20881 tacaaaacca acctgttgct cagccgaccg atattgatgg gacttatact ggtcaggatg
    20941 acggagaccg tatcacttta gtggtaactg gaacgactgg tacatggact gagctcgaat
    21001 ctgacgggga tcagaaagtc aaacaggtta catttgattc agcaaatcaa cgcatgatta
    21061 ttggcgatga tgtcaaaatt tacactgtaa acggtaatca aatcgtcgta gatgatatgg
    21121 atagagaccc atcggaccaa atcgttttaa ctaaataaga ctatgcaagt agaaagggct
    21181 ggatttttca gcctttttac ttttacctag aaaataatca taaatacttg ccttctaccg
    21241 aatacctgcg ttatactagt atcaattact tgtttttagc attcaaacta tcaaggaggg
    21301 gatatgaaat ataggaaatt tcaattattg atgtccaagt atggctttag tctttcgatt
    21361 atgttacttg aactttgtct tgtttttggt ctctttcttt atttaggacg catggctccc
    21421 attttatgga ttactgtcct cattctactg agtatcatca caatcatttc gatagtcaac
    21481 cgtaatacga ctcctgagaa taaggtaacc tggttgttag tagcctttgt gccagtattt
    21541 ggtcccttgc tctatctgat gtttggtgaa aggcgattgt ccaaaaaaga aatcaaacaa
    21601 ctgaagaagc taggctctat gcatttccaa gaagcaaata gccagctact aaaagagaaa
    21661 ttaaaagaaa gtgacaaggc agcttatgga gtcatcaagt ccttattgag tatggatacc
    21721 aatgctgaca tctatgatca aactgcctct acattttttc ctaacggaga agctatgtgg
    21781 aaaaagatgg tagaagatct taaaaaggct gagaaattta ttttcttgga atattacatt
    21841 atagaagaag gtttgatgtg gaatcgcata ctagatatac tagagcaaaa ggtagctcag
    21901 ggtgtagagg ttaagatgct ctatgatgat atcggctgta tggctacttt aacaggagat
    21961 tatgcacatc gacttcgtca gctgggcatc gaggcccata aattcaataa agttattcct
    22021 cgtttgacag tggcttataa taacagagat catagaaaaa tattgattgt tgatggtcag
    22081 atagcctata ctggtggggt caatctggca gatgagtaca ttaaccacgt cgagagattt
    22141 ggttattgga aggatagtgg aattcgctta gacggactag cagtaaaagc tctgacacgc
    22201 ttatttttga ccacttggta cattaatcga ggagaaatta gtgattttga tcaatatcat
    22261 ttagaaaatc attctatccc gagtgacggt ttaaccattc catacggaag tggacccaag
    22321 ccaatttttc gagcgcaggt agggaaaaaa gtttatcaga gtttaatcaa tcaagcaaca
    22381 gaatcggtct atattacgac accttatttg attatagatt atgatttaac agagacaatc
    22441 aaaaatgcag ctatgagagg ggtcgatgtt cgaattatca ccccttacat accagataag
    22501 aagttcattc agttagtcac gagaggagct tatcccgacc ttctttctgc tggtgttcgg
    22561 atttatgagt atagtccagg ttttattcat agtaagcaga tgttggtaga cgaagatttt
    22621 gcggtggtgg ggacaatcaa tctcgactac cggagcttgg tacaccatta tgaaaatgca
    22681 gtcttactct ataaaactcc ttctataagg gaaatcgccc gagattttcg aaatatattt
    22741 gcagattctc aggaagtcta tcctcattct atcaaaacga gctggtatca aaagcttgta
    22801 aaagaaatcg cccagctatt cgcccctatc ttataagaaa tctagaactt aggatacaaa
    22861 ccagacctat ttttcttgtc ttttacgaat agaaagaaag aggaggggaa ttcatgctaa
    22921 ctaaggaaga agtgaatgat atgattgagt tcaaacagac acatttacac cgctttcggg
    22981 aaattgaaac ctttgtgaaa ctatgcaaaa aatcactcaa acagccatcg caagctgaca
    23041 atcctaggac tttttgatat actaagacag ataaattgaa caggagaaac gatatgagtg
    23101 tgtatggtag agtagaagaa gttcataagg agaatcgtga acctctagaa taccaaatcg
    23161 aacaagaatc gcatcatcgt gaatcaagtc gtcttccttt ggtgaaaatt ttactatgga
    23221 gtacgcttgt aacggggata actctaggag tgccgctatt actcgactta atgagtgcac
    23281 aagaagtgca ggatttttat gcaggttggg cgcttcatca gacagggaag atttacagcg
    23341 actattatgg aagtcaaggt ttgctttatt atttgctgac ttacgtgagt cagggcggat
    23401 ttttctttgc catctttgag tggttagcct tggtagcagg aggatttttc ctttttagat
    23461 cagcggacac cttgacagag caaggagacc aagctggaca tctggtgact attttttaca
    23521 tgctagttac aggtcttgct tttggtggag gctatgcgac tcttttagcg cttcctttct
    23581 tattcgcagc ctttagttta gttgcggctt acctaagcaa tccaagccat gataagggat
    23641 ttgtacggat tgggctagct ttggcaggcg gatttttctt tgctccctta tcatcgctcc
    23701 tgtttattgc tgtagtgagt ttaggcttgt tggtctttaa ccttgggcat agacgttttg
    23761 tgcatgggtt ttatcagttt cttgcagtgg ctttaggttt ttcacttgtc ttttatccaa
    23821 ctgcctacta tagtgctgca acaggaagtt ttggggatgc gattagtggt attcgttatc
    23881 ctattgacag tattcgcttt gattttactt ctaaaatttt agagaatatg tttttttatg
    23941 gcttgttgtc ccttggtttg ggatttgtgg tttgtatctt tttaggtctc tttcaatcca
    24001 aaccctttaa actatacgtc atttcggttc ctgcaagtct tgtggtaatt ttaggtttga
    24061 ttttactttt cttttcacaa gagcctctgc acgcttctta tttgatggtc gtcttccctg
    24121 ttttcctact tttattggta accaatatta agagtcaaca gagggggcgt agtgctagaa
    24181 gaagccgaag agaaacgcca gttagcctat ggagtcgttt cttcaaagga aatctatatc
    24241 tgctagtttt tgggtttgtc tatcttttgt ctgttccttt tttgatgaag tttgtccttt
    24301 atccagtacc ttatcaagaa cgtaatcgtc ttgctgattt ggtaaaagag gagacaaata
    24361 cggaagatgc tatctatgca tgggatgata ctgcgactct ttatcgtaag agtgagcgct
    24421 tgtcgccatc ggcgattttg tccccgttgc actatacagc aactgaggaa aatcgtaata
    24481 agttacttaa tgacttgaaa gaaaaacaac ctaaggtgat tgtggtaaat gacaaggtgg
    24541 tagtctggtc tgaagtggaa acactcttaa aagaaaatta ccaacaagta aagactgatt
    24601 actcagagtt taaagtctat aaaattaaat aaccaaatca atatcttgtg tatttttaaa
    24661 aattttagga t
//
LOCUS       DAVKGC010000020        24280 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKGC010000020 DAVKGC010000000
VERSION     DAVKGC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.020"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            94..390
                     /locus_tag="VW049_000648"
     CDS             94..390
                     /locus_tag="VW049_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9431813.1"
                     /translation="MDTPDENGYVADNYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDLVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIDSNGASIG"
     gene            511..1455
                     /gene="manA"
                     /locus_tag="VW049_000649"
     CDS             511..1455
                     /gene="manA"
                     /locus_tag="VW049_000649"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9431814.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1446..2828)
                     /locus_tag="VW049_000650"
                     /pseudo
     CDS             complement(1446..2828)
                     /locus_tag="VW049_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821651.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3034..3779
                     /locus_tag="VW049_000651"
                     /pseudo
     CDS             3034..3779
                     /locus_tag="VW049_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3808..4263)
                     /locus_tag="VW049_000652"
     CDS             complement(3808..4263)
                     /locus_tag="VW049_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9431815.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4283..5458)
                     /locus_tag="VW049_000653"
     CDS             complement(4283..5458)
                     /locus_tag="VW049_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9431816.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5517..6362)
                     /locus_tag="VW049_000654"
     CDS             complement(5517..6362)
                     /locus_tag="VW049_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9431817.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6487..7044
                     /locus_tag="VW049_000655"
     CDS             6487..7044
                     /locus_tag="VW049_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9431818.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7063..7530
                     /locus_tag="VW049_000656"
     CDS             7063..7530
                     /locus_tag="VW049_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9431819.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7619..8248
                     /gene="upp"
                     /locus_tag="VW049_000657"
     CDS             7619..8248
                     /gene="upp"
                     /locus_tag="VW049_000657"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9431820.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8422..9012
                     /gene="clpP"
                     /locus_tag="VW049_000658"
     CDS             8422..9012
                     /gene="clpP"
                     /locus_tag="VW049_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9431821.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9091..9339
                     /locus_tag="VW049_000659"
     CDS             9091..9339
                     /locus_tag="VW049_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9431822.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9440..10600
                     /locus_tag="VW049_000660"
     CDS             9440..10600
                     /locus_tag="VW049_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9431823.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10868..11737
                     /locus_tag="VW049_000661"
     CDS             10868..11737
                     /locus_tag="VW049_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9431824.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11741..12697
                     /locus_tag="VW049_000662"
     CDS             11741..12697
                     /locus_tag="VW049_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9431825.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSTSPTYGAFFGAMLVGALLSG
                     VVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12697..13461
                     /locus_tag="VW049_000663"
     CDS             12697..13461
                     /locus_tag="VW049_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431826.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13461..14171
                     /locus_tag="VW049_000664"
     CDS             13461..14171
                     /locus_tag="VW049_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431827.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKIPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14479..15135
                     /locus_tag="VW049_000665"
     CDS             14479..15135
                     /locus_tag="VW049_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9431828.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIISL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15243..16338
                     /gene="prfB"
                     /locus_tag="VW049_000666"
     CDS             join(15243..15314,15316..16338)
                     /gene="prfB"
                     /locus_tag="VW049_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9431829.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16356..17048
                     /gene="ftsE"
                     /locus_tag="VW049_000667"
     CDS             16356..17048
                     /gene="ftsE"
                     /locus_tag="VW049_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9431830.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17041..17967
                     /locus_tag="VW049_000668"
     CDS             17041..17967
                     /locus_tag="VW049_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9431831.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTEKLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            18253..20433
                     /locus_tag="VW049_000669"
     CDS             18253..20433
                     /locus_tag="VW049_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9431832.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVTAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20485..21300
                     /locus_tag="VW049_000670"
     CDS             20485..21300
                     /locus_tag="VW049_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9431833.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            21440..22783
                     /locus_tag="VW049_000671"
     CDS             21440..22783
                     /locus_tag="VW049_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9431834.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            22998..24188
                     /locus_tag="VW049_000672"
     CDS             22998..24188
                     /locus_tag="VW049_000672"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9431835.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELIPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
BASE COUNT         7406 a         4544 c         5318 g         7012 t
ORIGIN      
        1 gtttggattt cttctttagt tgatttcata tcactattat ataatgcttt ttgattttag
       61 tttagtatta tcgtagaaaa tggactcggt gctatggata caccggatga gaatggttat
      121 gtagcagata actatcggat tacttactta gaggcccaca tcaaggccat gcgagatgcc
      181 atttaccaag acggggttga cttgcttggt tatacgactt ggggctgtat cgatctggtt
      241 tcagctggca caggggaaat gaataagcgc tatggcttta tctatgtaga tcgagataat
      301 gtaggaaacg gagctctcaa acgtagcaag aagaagtcct tctattggta caaggatgtc
      361 attgatagca atggtgcaag catcgggtag agcacatttg tttactattt ttataaaatc
      421 ttctccctgc tttataagat gtaaaaaaga gttcaattcg aactcttttt ttgctataat
      481 gaggggagaa aaatcagaca ggagatcgac atgtcagaac cattattttt acaatcagtt
      541 atgcaagaaa aaatctgggg tggagccaag ctacgtgatg agtttggcta cgacatccca
      601 agtgaaaaaa tcggagaata ttgggccatc tcagcccatc caaatggagt ctctaaagtt
      661 gccaatggtc gttacgaggg aacagatctt gctactttgt atgcggaaca ccgtgaatta
      721 tttggcaatc gtccagaacc tgtatttcca cttttgacca agatcctcga tgccaacgac
      781 tggctcagtg tccaagttca cccagacgat gcttatggac tcgagcatga aggcgaactc
      841 ggaaaaacag aatgctggta cattatcgca gcggatgaag gttcagagat tatctacggt
      901 cacaatgcca agtcaaaaga agaactccgc cagcaaatcg aggataagaa ctgggatgcg
      961 ctgttgacaa aagtgccagt taaagcagga gatttcttct atgtaccaag cggtaccatg
     1021 cacgctatcg gggcgggtat cttgattctt gaaacccagc agtctagcga taccacttac
     1081 cgtgtctatg actttgaccg taaggatgac aagggcaatt tacgtgaact tcaccttgaa
     1141 aaatctatcg atgtcttgaa cattggtgaa cccgcaaata gccgtccagt gacaatcaaa
     1201 gcagatgatc tacgttccac tctccttgta tccaatgatt tcttcgcagt ttacaaatgg
     1261 gaaattactg gaaaagttaa ctttgaaaag acagctgact acagcttgtt gagtgtcttg
     1321 gctggtcaag gtcaactaac tgttgacggg aaaaactatc caatccaaaa agggagacac
     1381 tttatcctac caagtgatgt tgaagcttgg actctggaag ggcaagattt agaattaatt
     1441 gttagtcatc cataaaaaag aaagggcctg agttcattac tcaagtcctt ttgattacat
     1501 aaattgggcg atgaagacca cgatgatgat tggaatgacg gaacgaagaa acgaagaagg
     1561 aagagccaga cttggaacag tccttgtttc catgctcttt catcgagatg gagttcctcc
     1621 attgcaagag cctttttaaa gatatagcct gtaaaaagtg aaaggtagag agctccaaat
     1681 ggcatgagga gattggaaac caagaagtcc atagcgtcaa agaaggtctt accaaaaatg
     1741 tgaacatccg ccatgacacc gtaagatagg gctgaaggaa tgccaaagac aaaggtcaaa
     1801 attcctaaaa taacactcca tttggcacgt ttgctgttat cctggttggt gatattgccc
     1861 acattgatct ccagcatcac gacagaagaa gtgacagtcg caaagaggaa gaggacgtag
     1921 aaaatggttc caaaaggcat cttgtcaaag agttgaggca agacgataaa gagcaagctc
     1981 ggtccccctt cagactggat attgaagggg gatcgagctt ggaaaatggc tagacctgcc
     2041 atgatggata tcgagatatt catggctacg atggagattc ctgactggac tagattggtt
     2101 ttcttgtcca agtaaggagc ataggtcaac atgactgtaa cccctagtga gagggcaaag
     2161 aaagattgtc ccagagcata gaggagacca gcgctagtca gttttgaaaa gtctggtttg
     2221 aggaagtaaa gaacccttcc atggcatttg gcaaactgag agagcgaccg atgataacaa
     2281 caaagatgat aaagagcagg ggcatcatga ctttcgaagc tctttcaatc cctttttgaa
     2341 caccacgtga tacaataaag atatttaata agataaaggc tgcttgagct actagggcaa
     2401 tggctggatt tgaaatgatt gaagtaaata actgagcata atcacccgtt ccaccaagtt
     2461 ggaacaattt cccaaactca atacctagat agactagaat ccatcctccg ataacactgt
     2521 aaaaagataa gaggataaag agggcaaagg cgccaatcca accgataaag ttgtacttgt
     2581 tattcttgcc cagttttcca aaggttttga tagcggaaac gccagccact acggccaagg
     2641 gcaaactcag ccagcaggag agggaaaccg attaaaatag tggaaatgag aaagattagt
     2701 aaaaagcctc caccgccatt agcagcagtc atgtagggaa acttccaaac ggaaccaagc
     2761 ccgatgaccg agccagcaga tgctagaata aagccaagtt tcgaccccca ttgtgatttt
     2821 tctgacatag ttaaactctg atctcctttt tcaaaataag aaaaagctac ttgagttgtc
     2881 aaatagcctt ctgtttgatt gatagacttg actatcctat tatgttttct aaggtctgtc
     2941 aagaaaacca ttttcaagtt ttcacacctt tctcaaaaac gcttgactct gacctaaggg
     3001 gaggggttat actatcattg taaggaggaa atcatgtacc atataaaaga agctgcgcag
     3061 ctttcaggtg tctctgtcaa gaccctgccc tgcatcacta tgacaagata ggactcttgg
     3121 tccccttaaa gtcggaaaac ggctatcgaa cctatagtca agaggatttg gaacgccttc
     3181 aggtcattct ttactacaaa tatctaggct tttctttaga gaaaatagca gaactgttaa
     3241 aggaagaaag gacagattta ttgccccatt tgactaggca gttggactat ctaactcgcg
     3301 aaaggcaaca tctggatacc ttgatttcca ccttgcaaaa aactattcaa gaacaaaaag
     3361 gagaaagaaa aatgaccatt gaggaaaaat tcacgggatt tagctatcaa gacaatcaaa
     3421 aataccacca agaagcggta gagaaatatg gtcaagaagt catgggacaa gcgctcgaac
     3481 gccaaaaagg tcacgaagac gaggctacgg ccgcctttaa ccaagtcttt caaactttgg
     3541 cacaaaatct tcaagttggt ttacctgcaa cagcaaccga aaaccaggag caagcagcca
     3601 agctcttgca agccattcgc acttatggat ttgactgctc tattgaggta ttcggtcata
     3661 tcggtaaagg ttacgtctac aacccagagt ttaaggaaaa cattgacaag tttggttctg
     3721 aaacagccca gtacacgtca gatgccattg cggcttacgt tcagacaaat gcagaataaa
     3781 taggctagga atttcctagc ctatttttta cttcaaatca taaagccagt cgtcaccgtt
     3841 tttgtagtaa aagaattcac tgagatcttc ttctagaaac acacgaagca tatcagacat
     3901 atcatcggtt gcaagtttta gatgagaaag attttcaaag tcctcccacc aaactttccc
     3961 ttcgtctgaa gactggagtt caccagtaaa gtgttctgtc ttgtaaaaaa ggacgacata
     4021 acgataatcc ttgtcgtcat accagttttt gataccacag agttggggtt tggaaatgat
     4081 cagaccagtt tcttctttca cttcacgaat gacagcatcg acaaaggatt cgccacgttc
     4141 aacatgacca ccaggaaaag taatgccaga ccagtcggga ctaactcggt cttggaccag
     4201 gaccttatct ccgtttttaa tcatacacat gttaacaaat tcgactgcct ctcttctgtt
     4261 cattcttcac aacctttaat ctttaatcat aatgcagact tcccgccacc cagccggtac
     4321 agagggcaga agtgatgtta aagccacccg tgtgggcatt gatatccata acttcgcctg
     4381 caaagtggag gccaggtacc agcttacttt caagggtttt aggattgatt tccttgagac
     4441 tgactccacc cttggtaaca aaggactttg caagggacat ttttccagtt acaggaattt
     4501 taagttcttt aatggactgg acaagttgtt ctcgttcctt ttcagtcagt tgtttgactt
     4561 tttcaggata tccttgtaca aaaaattcgg ccaagcgttc tggtaacaag gtttttaaag
     4621 cgtttttcaa ggatttttcc cgattttctt ctagaaatgt aaccaagtcc ttctcagaaa
     4681 gttgaggcaa aacatcgagt gagagaacct ccccaccttt gacaaagcta gacatgcgta
     4741 gggcagcagg acctgacaaa ccaaagtggg taaagagtaa atcatgagtg atgacatgct
     4801 taccataact tagggtcaca tcgtccagag aaatcccttg taaggcttta tgtggaaaat
     4861 ctgttaataa aggactttca gcagcctcaa gatcggtgat ggtatgctta aaatggcgag
     4921 caatctcgtg accaaaacca gtcgaaccag tcgaaggata agacttacca cctgttgtga
     4981 caatgagttt ctcacaagtg aaggtttgat ccgctgactt aaggacaaac tggtcatcta
     5041 cttttttaac agaaacgatt tctatttgag tagcaacttg accacctagt tcggtgattt
     5101 tcttttccaa agcttcgata atagtccgag acttgtcact ggctggaaag acgcgtccgt
     5161 ggtcttcgac cttaagttta acaccatttt ctgtaaaaaa gttgatgatg tcatgattat
     5221 cgaactggga gaaaacactg taaagaaagc gtccgtttcc aggaattcca gctagcaggt
     5281 tgtctaagct accattgttg gtcacattgc aacgtccccc accagtccca gctaattttt
     5341 ttccaagttt ccgatttttt tcgatgagga gggttttctg tccataaaag ctactggaaa
     5401 tcgtagccat cataccagca ggtcccccac cgatgacaat agtatcaaaa tgtttcatag
     5461 ctctattgta ccacaaaaaa acaagagatg atggtcacct cttgtcaaga atgcaattaa
     5521 tcaatttcat agcccatcag caaaccgccc tcttctgcat agaaactgca gagaccagag
     5581 gttggtagaa ttttaatatc cgcttgtggg aaggtttcac ggattcgctc tgagagctgt
     5641 tgacaacatt tttcgttatt gcgttgggcc atgacaatac ggccaccagc atatccagct
     5701 tttactaact catcataggc agcttgaact gatttctttg atccccttgc tttttgtagc
     5761 aattcgagag tcccagtttc actagctttt ccgaccatac gaatgttgag aaggccaacg
     5821 accgtaccga taagcttgct caaacggccg ttcttcacca agttatcgac tttggctagg
     5881 acaaagagca acttagtttt ttcttgatag gcggtgatag cttcaaccac ttcttcaaaa
     5941 gacaagccct ggtcaatcaa gtcattcaat ttttctacga gtaggtcaac ttcaccacca
     6001 gcagataaac tatcaatcac atgaatctta gtgtcaggat ggtcttccag ataaatattc
     6061 tttgctagtt gagcactatt gtgactgcca gaaagggtac ctgtgatggt tactaggaaa
     6121 atgtttttgg caccttcaaa tgctcgcaaa tagtcatctg ggcttggaca agctgatttt
     6181 gaagcttctg cagttgcata catggtttcc atcatttggt caatatcgag actggcgtca
     6241 tcaacaaaga cctgatcagc tacttgaatg gttaagggga cacttacaaa ggttgtgtta
     6301 atagctggtg ttggcagttg acgataatca caaccagagt cagcaataat cttccaagtc
     6361 atagaaattc tccatctttg tcagttatac attgacaaag gttctgtctt tttttacaat
     6421 tatatcatga aaactcttga aacaaaagca tcatccgtct gttgtgacaa gtagaaagaa
     6481 aatgttatgt ctgaacgtag aatctctgaa aagtctcttg aaaatctcag aaaatcaaac
     6541 caagaatcca atttattaac cagagaagcc attgaaacag ccctcttgca actcttggaa
     6601 aaaaaggagc tgaccaagat tagtatttct gaattggtca aacgtgcagg tgtttcgcgt
     6661 gcggcctttt atcgcaatta tgactccaaa gaggaaattt tagagagcgt ctttaaacga
     6721 actgtccaca atattatgga acagctgcat cattacgatt taaagacaga cctttatttg
     6781 gtttgggttc accttttccg ggaggccaga aaggaagcta gagtaattca attggccttg
     6841 gattaccatc tggaaaaaat ctttgtccaa gccatgcagg aatttctaga aaaataccat
     6901 gggaaatcaa aaggtgtcag ctcttatctt cattccttct ggagctcagc catcgtctct
     6961 gtccttctaa aatggatcaa ggatggcatg aaggtaccag ctgaaaagat tgcagattta
     7021 ggtttaccat tttttaaaaa atagagaaaa aggagaaaag agatgactga aaaaagactg
     7081 gcctgggatg agtattttgc agcccaagct ctactaattg cgaatcgttc cacttgtaaa
     7141 cgtgctaaag tgggcgcgat tctggtaaaa gataataagg ttatttccac tggttacaat
     7201 ggttcggtgt cagggactga gcattgtatt gatcacgaat gtttggtcat tgaaggccac
     7261 tgtgttcgca cccttcacgc tgaggtcaat gctatccttc aaggtgctga gcgtggtgtt
     7321 cccaaaggct ttacagccta tgtaacccat tttccttgtc tcaactgtac aaaacaattg
     7381 cttcaggtcg gctgcaagcg cgtggtttat atcaaccagt accgaatgga cgactacgcc
     7441 caataccttt atcaagaaaa agggacagaa ttgacccatt taccacttga gacagtacag
     7501 acagctctta aagaggctga tctaatgtaa aaattatcaa aaataaatgg tttagaaaga
     7561 tttttaaacc gttttttggt ataataagaa gaataaattg aaagaaggaa ttccaaaaat
     7621 gggaaaaatt gaagttatta atcacccact gattcaacac aaattgtcaa tcttgcgtcg
     7681 tacagatact tctacaaaag cttttcgtga gctagtagat gagattgcaa tgttgatggg
     7741 gtatgaagta cttcgtgatc ttccactaga agatgtggaa atcgaaacac caattacaaa
     7801 aacagttcaa aaacaattgg caggtaagaa attggccatc gtcccaatct tgcgtgcagg
     7861 tatcgggatg gttgatggtc tcttgagctt ggttccagct gctaaagttg gccacatcgg
     7921 tatgtaccgt gatgaagaaa cacttcaacc agttgaatac ttggtgaaat tgcctgagga
     7981 cattgaccaa cgtcaaattt ttgtagtaga cccaatgttg gcaacaggtg gctcagcaat
     8041 cttggctgtt gattctctta aaaaacgtgg cgcatcaaat atcaaatttg tctgccttgt
     8101 atctgctcca gagggtgtaa aagcccttca agaagctcat ccagatgtag aaatctttac
     8161 agcagccttg gatgaacgtt tgaacgaaca cggttatata gttccaggtc ttggagatgc
     8221 tggagaccgc ttgttcggta caaaataaga tcgaaaagaa gaatgacttg gaaaagtatt
     8281 tctagtcacg aaaggaggtt gggtttttgt ttctgtctaa tgaaagcaga gcaaaaattt
     8341 gacctttttt gaccaagata ttataatagt cttatcttca gtcatttgac caacaaaatt
     8401 aaaactcaaa aggagaaatg aatgattcct gtagttattg aacaaacaag ccgtggagaa
     8461 cgttcttacg atatttactc acgtcttctc aaagaccgca tcattatgct gacaggtccg
     8521 gttgaagaca atatggctaa ctctgttatt gcccaattgc ttttcttgga tgcccaagat
     8581 agtacgaaag atatttacct ttatgtcaat acaccaggtg gttctgtttc agctggtttg
     8641 gcaatcgtag ataccatgaa ctttatcaag gcagatgtcc aaaccattgt tatgggaatg
     8701 gctgcatcta tggggactgt catcgcatca agtggagcaa aaggcaaacg tttcatgctt
     8761 ccaaatgctg aatacatgat tcaccaacca atgggcggta caggtggtgg tacccaacaa
     8821 actgatatgg ctatcgctgc agaacacttg ctcaaaactc gtaatacctt ggaaaaaatc
     8881 ttggctgaaa attcaggtca gtcaatggaa aaagtccatg cagatgcaga acgtgataac
     8941 tggatgagcg cccaggaaac acttgaatat ggctttattg atgaaattat ggccaacaat
     9001 tcattgaact aatgatgata gaaggcaaac tcgactgggt ttgctttttt tggtataata
     9061 gggagagatt tcttagaaag aggatttatc atgtttgaaa aagtcaatcg ctctggcttg
     9121 attatctatc tttactataa tcgtgatgcc aaaaaactgc aggattatgg agatattacc
     9181 tatcattcca agaaacatcg ttacttacaa ctctatgttc caactcaaga agtggagcaa
     9241 ttggtcggac gcttgagcaa ggaaaaattt attaaaaaag ttagggtttg tcatatccaa
     9301 gagttggaaa caccctttgt gggcaatctt tatcgatagg aaaacgttat catcgaaaaa
     9361 gttcaaggaa aacattgaca attttctgat aattcggtat attcttaaca gactatttaa
     9421 gaaataagga gacaaaaaga tgaagaaaaa atttgcccta tcgtttgtgg cgcttgcaag
     9481 tgtagcactt cttgcagcct gtggagaagt gaagtctgga gcagtcaaca ctgctggtaa
     9541 ctcagtagag gaaaagacaa ttaaaatcgg gtttaacttt gaagaatcag gttctttagc
     9601 tgcatacgga acagctgaac aaaaaggtgc ccaattggct gttgatgaaa tcaatgccgc
     9661 aggtggtatc gatggaaaac aaatcgaagt agtcgataaa gataataagt ctgaaacagc
     9721 tgaggctgct tcagttacaa ctaaccttgt aacccaatct aaagtatcag cagtcgtagg
     9781 acctgcgaca tctggtgcga ctgcagctgc ggtagcgaac gctacaaaag caggtgttcc
     9841 attgatctca ccaagtgcga ctcaagatgg attgactaaa ggtcaagatt acctctttat
     9901 tggaactttc caagatagct tccaaggaaa aattatctca aactatgttt ctgaaaaatt
     9961 aaatgctaag aaagttgttc tttacactga caatgccagt gactatgcta aagggattgc
    10021 caaatctttc cgcgagtcat acaagggtga aatcgttgca gatgaaactt tcgtagcagg
    10081 tgacacagac tttcaagcag cccttacaaa aatgaaaggg aaagactttg atgctatcgt
    10141 tgttcctggt tactataatg aggctggtaa gattgtaaac caagcgcgtg gcatgggaat
    10201 tgacaaacca atcgttggtg gtgatggatt caacggtgag gagtttgtac aacaagcaac
    10261 tgctgaaaaa gcatcaaaca tctactttat ctcaggcttc tcaactactg tagaagtttc
    10321 agctaaagct aaagccttcc ttgacgctta ccgtgctaag tacaatgaag agccttcaac
    10381 atttgcagcc ttggcttatg attcagttca ccttgtagca aacgcagcaa aaggtgctaa
    10441 aaattcaggt gaaatcaagg ataaccttgc taaaacaaaa gattttgaag gtgtaactgg
    10501 tcaaacaagc ttcgatgcag accacaacac agtcaaaact gcttacatga tgaccatgaa
    10561 caatggtaaa gttgaagcag cagaagttgt aaaaccataa tagaaaaatg ttgaaatagg
    10621 gaatgagcct ttgactcact ccctgtttcg atatttaata ctcttcgaaa atctcttcaa
    10681 actgcgtcaa cgtcgccttg gattatatat gtgactgact tcgtcagtct tatctacaac
    10741 ctcaaagcag tgctttgagc aacctgtggc tagtttccta gtttgctctt tgattttcat
    10801 tgagtataag aacctatcaa aaagtgaggg aaacccctcg gaattataaa tagaaagagt
    10861 gaatcttatg ctacaacaac tcgtaaatgg tttgattcta ggtagtgttt acgcgctgtt
    10921 agccctagga tataccatgg tttacggaat tatcaagctc atcaacttcg cccatggtga
    10981 tatttatatg atgggagcct ttatcggtta tttcttgatc aattctttcc aaatgaattt
    11041 ctttgtagcg cttattgtag ctatgctagc gacagctatt cttggtgtcg tgattgagtt
    11101 tcttgcttac cgacctttgc gccactctac tcgtattgct gttttgatta cggctattgg
    11161 ggtttctttc ctattggagt atggaatggt ctatctggtt ggtgccaata cccgtgcctt
    11221 ccctcaagcg attcaaacag ttcgatatga tttgggacca attagcttaa caaatgtgca
    11281 gttaatgatt ttggccattt ccttgatttt gatgattttg ttacaagtca ttgtccaaaa
    11341 gactaagatg gggaaagcca tgcgtgcagt atcagtagat agcgacgcgg cgcaattgat
    11401 ggggatcaat gtaaaccgta cgattagctt taccttcgct ttgggttctg ctcttgcggg
    11461 tgcggctggt gttctgattg ctctttatta taactctctt gagcctttga tgggggttac
    11521 tccaggtctt aaatctttcg ttgccgcagt acttggtggt atcggaatta ttcctggtgc
    11581 ggctcttggt ggctttgtga ttggtctatt ggaaaccttt gcgactgcct ttgggatgtc
    11641 agatttccgt gatgccattg tttatggaat cttgttgttg atcttgattg tccgcccagc
    11701 tggtatcctt ggtaagaatg tgaaagagaa ggtgtaaacg atgaaggaaa atttaaaagt
    11761 taatattcta tggttactcc ttttgttagc tggctatagc ttgattagtg tactggtttc
    11821 agtcggagta cttaatctat tctatgtaca gattttacaa caaattggaa ttaatattat
    11881 tttggctgtt ggtctcaact taatcgttgg tttttcagga caattttcac ttggtcatgc
    11941 tggtttcatg gcgattggtg cctatgcagc agctattatt ggttctacat caccaaccta
    12001 cggtgccttc tttggagcta tgcttgtagg ggctttgctt tcaggagtag ttgccttact
    12061 tgtcggcatt ccaaccttgc gcttgaaggg ggactatctt gcggtagcaa ctctgggtgt
    12121 ttctgaaatt atccgtatct ttatcatcaa tggtggaagt cttacaaatg gtgcggcagg
    12181 tatcttaggg attcctaact ttacaacttg gcaaatggtt tacttctttg tcgtgattac
    12241 aaccattgca accttgaact tcttgcgtag cccaattggc cgttcaaccc tctctgttcg
    12301 tgaagatgaa atcgctgctg agtcagttgg ggttaatacg actaaaatta aaatcatcgc
    12361 ttttgtcttt ggtgccatta ctgcaagtat tgctgggtca cttcaggcag gatttatcgg
    12421 gtctgttgta ccgaaagatt acaccttcat caactcaatc aacgttttga ttattgttgt
    12481 atttggtgga ctcggttcca ttacaggtgc gattgtttcg gctattgttc tgggaatttt
    12541 gaatatgctt ctccaagatg ttgctagtgt gcgtatgatt atttacgctt tggccttggt
    12601 attggtaatg attttcagac caggtggact ccttggaacg tgggaactga gcctatcacg
    12661 tttctttaaa aaatctaaga aggaggaaca aaactaatgg cattacttga agtaaaacag
    12721 ttaaccaaac attttggtgg tctaacagct gttggagatg tgactcttga attgaacgaa
    12781 ggggaactgg ttggattaat cggtccaaac ggagctggga aaaccaccct tttcaacctt
    12841 ttgaccggtg tttatgaacc aagcgaggga acagtaaccc tagatggtca ccttttgaat
    12901 gggaaatcac cttataagat tgcctctttg ggacttggac gtactttcca aaatatccgt
    12961 ctttttaaag atttaacagt tttagacaat gttttgattg cttttggaaa ccatcacaaa
    13021 cagcatgttt ttactagttt cttacgctta ccagcttttt acaagagtga aaaagaatta
    13081 aaggctaaag ctttggaatt gttgaaaatc tttgatttag atggtgatgc agagactctt
    13141 gctaaaaatc tttcctacgg acaacaacgt cgtttggaaa ttgttcgtgc ccttgctacg
    13201 gaacctaaaa ttctcttctt agatgaacca gcagcaggta tgaacccaca ggaaacagcc
    13261 gaattgactg agttaattcg tcgtatcaaa gatgaattta agattacaat catgttgatt
    13321 gaacacgata tgaatctggt catggaagta acagaacgta tctacgtact tgaatatggc
    13381 cgtttaattg ctcaaggaac tccagacgaa attaagacca ataaacgcgt tatcgaagct
    13441 tatctaggag gtgaagccta atgtctatgt taaaagttga aaatctttct gtgcattacg
    13501 gtatgatcca agcagtccgt gatgtaagct ttgaagttaa tgaaggagaa gttgtttccc
    13561 ttatcggtgc caacggtgca ggtaagacaa ctattcttcg caccttgtca ggtttggttc
    13621 gaccaagttc aggaaagatt gaatttttag gtcaagaaat ccaaaaaata ccagctcaga
    13681 aaatcgtggc aggtggtctt tcacaagttc cagaaggacg ccacgtcttt cctggcttga
    13741 ctgttatgga aaatcttgaa atgggagctt tcttaaagaa aaatcgtgaa gaaaatcaag
    13801 ctaacttgaa gaaggttttc tcacgctttc ctcgtcttga agaacggaag aaccaagatg
    13861 cagctactct ttcaggaggg gaacaacaaa tgcttgccat gggacgcgct cttatgtcaa
    13921 caccaaaact tcttctttta gatgaaccat caatgggact tgccccaatc ttcatccaag
    13981 agatttttga tatcattcaa gatattcaga agcaaggaac aaccgtcctc ttgattgaac
    14041 aaaatgccaa taaagcactt gcaatctctg accgaggata tgtactggaa acagggaaaa
    14101 tcgtcctatc aggaacagga aaagaactcg cttcatcaga agaagtcaga aaagcatatc
    14161 taggtggcta aaacaatcca gtggattgtt ttagtcggca gatggagatt acgaagtaat
    14221 catcaatata gtccggggga cctttttagt cggtagattg agattgcaaa caaatctgca
    14281 tctacattga aagcttaatt tctaataatt gaaaaaatcg aatgaaaagt ttcttacctt
    14341 cattcacaga gctcgatttt agagctcttt ttgctagctt attcatactt ttctaaattt
    14401 tgaaaaagaa atgtaagcgt ttgatagatt tgcaaaaaga ttgtataata gggataagaa
    14461 tagaaaagga gaagtctcat ggcagttaaa gattttatga cccgcaaggt agtttatatt
    14521 agtccagata caacagtatc tcatgcagca gatttgatga gagagcaaga attgcaccgc
    14581 ttgcctgtta tcgaaaatga tcaattagtt ggtttggtga ctgagggaac cattgcacaa
    14641 gcaagtccat ctaaagcaac aagtctttct atctatgaga tgaattatct tctgaataag
    14701 acaaaagtaa aagatgtcat gattcgcgat gttgtcactg tctcaggcta tgctagtcta
    14761 gaagatgcaa cttatctgat gttgaaaaat aagattggta ttctccctgt cgtagataac
    14821 catcaagtat acggagttat tactgaccgt gacgttttcc aagcctttct tgaaattgca
    14881 ggttatggcg aagaagggat tcgtgtacgc tttgttacag aagatgaagt tggtgttctt
    14941 ggaaaaatta tttctttgat tgtagaagaa aatttgaata tatcccatac agtcaatatt
    15001 ccgcgtaagg atggtaaggt cattatcgaa gtgcaaatcg atggatcaat tgatttacca
    15061 gccttgaaag aaaaatttga agcaaatggt attcaagtgg aagaaatcgc tcgcacttca
    15121 gcaaaagtct tgtaagaagg gaagcccaaa ggcttctttt ttcatgaaaa ggggattaga
    15181 gcaaaagatg gaaagaaatg ataaaatatg ctataatgaa ataatgtaaa aaaggagtat
    15241 ttatggacat ttcagtaatt cgtcagaaaa ttgacgcaaa tcgtgaaaaa ttagcttctt
    15301 tcagggggtc tctttgacct cgaagggcta gaggaagaga ttgccatctt ggaaaacaag
    15361 atgacagaac ctgatttttg gaacgataat attgcggccc aaaaaacgtc gcaagaattg
    15421 aatgaattaa aaaacactta caataccttc cataagatgg aagagttgca ggatgaagtc
    15481 gaaattttat tggatttttt ggctgaagac gagtcagtgc atgatgaact ggtagcgcag
    15541 ttagccgaac ttgataagat aatgaccagc tacgagatga ctctactctt gtcagaacct
    15601 tatgaccaca acaatgccat cttggaaatc catccaggtt ctggtggtac tgaggcgcag
    15661 gactggggtg atatgttgct tcgtatgtat actcgttatg gtaatgctaa aggctttaaa
    15721 gtggaagtgt tggattacca agcaggtgat gaggctggta ttaagtcggt aactttatca
    15781 tttgaagggc ctaatgccta tggtctcctc aagtcagaaa tgggtgttca ccgcttagtg
    15841 cgaatctcac catttgactc tgccaaacgt cgccatacct ctttcacatc tgtagaagtg
    15901 atgccagaat tggatgatac tattgaagtg gaaatccgtg aagatgatat caagatggat
    15961 accttccgtt caggtggtgc cggtggacaa aacgtcaata aggtttcaac aggtgtacgt
    16021 ttaactcaca ttccaactgg aattgttgtc caatcaacag tggatcgtac ccagtatgga
    16081 aatagagatc gtgccatgaa gatgttgcag gctaagctct atcaaatgga gcaagagaag
    16141 aaggctgcgg aggtagattc tctcaaaggt gagaaaaagg agatcacttg gggaagccaa
    16201 atccgttctt atgtcttcac gccttatact atggtaaaag atcaccgaac tagctttgag
    16261 gttgctcagg tagataaggt tatggatggg gatctagatg gttttatcga tgcttatctc
    16321 aagtggcgaa ttagctaaga tagaaaggaa ctcacatgtc aattattgaa atgagagatg
    16381 tcgttaaaaa atacgacaac ggaacaactg ctctacgcgg tgtttcggtc agcgttcaac
    16441 cgggggaatt tgcttacatc gtaggacctt caggagcagg gaagtcaact tttattcgtt
    16501 ctctgtatcg tgaagtaaaa atcgataaag gaagcctatc agttgctggt tttaatctgg
    16561 ttaagatcaa aaagaaagat gtcccgcttc tacgtcgtag tgttggggtt gtcttccagg
    16621 attataaatt gttaccaaag aaaactgtct atgaaaatat tgcttacgct atggaagtaa
    16681 tcggggaaaa tcgccgtaat atcaaaagac gagtgatgga agttttggac ttggttggat
    16741 tgaagcataa ggttcgttct ttcccaaatg aactctcagg tggggagcaa cagcggattg
    16801 cgattgcgcg tgcaattgta aataatccca aagtattgat agctgatgag ccaacaggaa
    16861 atctggatcc ggataattca tgggaaatta tgaatctctt ggaacggatt aacctacaag
    16921 gaacaactat tttgatggcg actcataata gccagattgt aaataccttg cgccaccgtg
    16981 tcattgccat tgaaaatggc cgtgtcgttc gtgacgaatc aaaaggagag tatggatacg
    17041 atgattagta gattttttcg ccatttattt gaagccttaa aaagtttgaa acgaaatggt
    17101 tggatgacag tagctgctgt cagttcagtc atgattactt tgaccttggt ggcaatattt
    17161 gcatctgtta ttttcaatac agcgaaacta gctacagata ttgaaaataa tgtccgtgta
    17221 gtagtttata tccgaaagga tgtggaagat aatagtcaga caattgaaaa agaaggtcaa
    17281 actgttacaa ataatgacta ccacaaggta tatgattctt tgaagaacat gtctacggtt
    17341 aaaagtgtta ccttttcaag taaagaagaa caatatgaaa aattaaccga gataatggga
    17401 gataactgga aaatctttga aggagatgcc aatcctctct atgatgccta tattgtagag
    17461 gcaaacactc caaatgatgt aaaaactata gccgaagatg ctaaaaaaat tgaaggtgtc
    17521 tctgaggttc aagatggcgg tgccaataca gaaaaactct tcaagttagc ttcatttatc
    17581 cgtgtttggg gactagggat tgctgctttg ttaattttta tcgcagtttt cttgatttca
    17641 aataccattc gtattaccat tatttcccgc agtcgcgaaa ttcaaatcat gcgcttggtc
    17701 ggagctaaaa acagttatat ccgtggaccg ttcttgttag aaggagcctt tatcggttta
    17761 ttgggagcta tcgcaccatc tgttttggtc tttattgttt atcaaattgt ttaccaatct
    17821 gtcaacaaat cgttggtagg gcaaaatcta tccatgatta gtccagattt atttagtccg
    17881 ttgatgattg ccctactatt tgtgattggg gttttcattg gttcattggg atcaggaata
    17941 tccatgcgcc gattcttgaa gatttaggta aaatagctgc ttttatgagg agattgtaaa
    18001 atctcctttt ttgctacaag agtttttgaa aagagatgcg cagaagaaaa gagcttccaa
    18061 agaagtcccc cagagaagac ttcttgatgg ctaatttgaa cttgaaattt ggcgcactag
    18121 aaatttttta gaaaaaagtg taattttttt ataaaagcct ttacaaaaaa aattaaagtg
    18181 gtataattaa atcatagaaa gtgcaaacgt tttcgtaaaa cgtttgccta aataaaaata
    18241 aaggagattt gaatgatgaa agatacattc aaaaatgtct tgtctttcga attttggcaa
    18301 aaattcggta aggctttgat ggtagttatc gcggttatgc cggctgctgg tttgatgatt
    18361 tcaatcggta agtctatcgt gatgattaac ccaacctttg caccacttgt catcacaggt
    18421 ggaattcttg agcaaatcgg ttggggggtt atcggtaacc ttcacatttt gtttgcccta
    18481 gccattggag gaagctgggc taaagaacgt gctggtggtg ctttcgccgc tggtcttgcc
    18541 ttcatcttga ttaaccgtat cactggtaca atttttggtg tatcaggcga tatgttgaaa
    18601 aatccagatg ctatggtaac tactttcttt ggtggttcaa tcaaagttgc tgattacttt
    18661 atcagtgttc ttgaagctcc agccttgaac atgggggtat tcgtagggat tatctcaggt
    18721 tttgtagggg caactgctta caacaaatac tacaacttcc gtaaacttcc tgatgcactt
    18781 tcattcttca acgggaaacg tttcgtacca tttgtagtta ttcttcgttc ggcaatcgct
    18841 gcaattctac ttgctgcttt ctggccagta gttcaaacag gtatcaataa cttcggtatc
    18901 tggattgcca actcacaaga aactgctcca attcttgcac cattcttgta tggtactttg
    18961 gaacgtttgc tcttgccatt tggtcttcac cacatgttga ctatcccaat gaactacaca
    19021 gctcttggtg gtacttatga cattttaact ggtgcagcta aaggtactca agtattcggt
    19081 caagacccac tatggcttgc atgggtaaca gaccttgtaa accttaaagg tactgatgct
    19141 agtcaatacc aacacttgtt agatacagta catccagctc gtttcaaagt tggacaaatg
    19201 atcggttcat tcggtatctt gatgggtgtg attgttgcta tctaccgtaa tgttgatgct
    19261 gacaagaaac ataaatacaa aggtatgatg attgcaacag ctcttgcaac attcttgaca
    19321 ggggttactg aaccaatcga atacatgttc atgttcatcg caacacctat gtatcttgtt
    19381 tactcacttg ttcaaggtgc tgccttcgct atggctgacg tcgtaaacct acgtatgcac
    19441 tcattcggtt caatcgagtt cttgactcgt acacctattg caatcagtgc tggtattggt
    19501 atggatatcg ttaacttcgt ttgggtaact gttctctttg ctgtaatcat gtactttatc
    19561 gcaaacttca tgattcaaaa attcaactac gcaactccag ggcgcaacgg aaactacgaa
    19621 actgctgaag gttcagaaga aaccagcagt gaagtgaaag ttgcagcagg ctctcaagct
    19681 gtaaacatta tcaaccttct tggtggacgt gtaaacatcg ttgatgttga cgcatgtatg
    19741 actcgtcttc gtgtaactgt taaagatgca gataaagtag gaaatgcaga gcaatggaaa
    19801 gcagaaggag ctatgggtct tgtcatgaaa ggacaagggg ttcaagctat ctacggtcca
    19861 aaagctgaca ttttgaaatc tgatatccaa gatatccttg attcaggtga aatcattcct
    19921 gaaactcttc caagccaaat gactgaagca caacaaaaca ctgttcactt caaagatctt
    19981 actgaggaag tttactcagt agcagacggt caagttgttg ctttggaaca agtaaaggat
    20041 ccagtatttg ctcaaaaaat gatgggtgat ggatttgcag tagaacctgc aaatggaaac
    20101 attgtatctc cagtttcagg tactgtatca agcatcttcc caacaaaaca tgcttttggt
    20161 attgtgacgg aagcaggtct tgaagtattg gttcacattg gtttggacac agtaagtctt
    20221 gaaggtaaac catttacagt tcatgttgct gaaggacaaa aagttacagc aggagatctc
    20281 cttgtcacag ctgacttgga tgctatccgt gcagcaggac gtgaaacttc aacagtagtt
    20341 gtcttcacaa atggtgatgc aattaaatca gttaaattag aaaaaacagg ttctcttgca
    20401 gctaaaacag cagttgctaa agtagaattg taatatactt gaggttggaa gctgtattcc
    20461 aacctcttat tttgggagaa aagaatgaaa tttttaacac tcaatactca cagttggatg
    20521 gagaaagaag cagaggaaaa attccagatt ttgcttgaag atattcttga aaaggactat
    20581 gatttgattt gtttccaaga aatcaatcag gagatgacct cgtcagaggt ggaggttaat
    20641 gacctttatc aagctttgcc agcagctgag cctattcacc aagaccatta tgttagactc
    20701 ttggttgaaa agttgttaga gcaagggaaa aattactact ggacctgggc ttataaccat
    20761 atcggctatg accgctacca tgaaggtgtg gctatcttgt ctaaaacacc tattgaagcc
    20821 agagaaattt tggtttcaga tgtggatgat ccaacagact atcatactcg ccgtgttgcc
    20881 ctagctgaaa ctgtagtcga tggcaaggag ctagcagttg ccagtgttca tctctcttgg
    20941 tgggataaag gtttccaaga agaatgggca cgatttgagg ctgtcttgaa aaaattgaac
    21001 aagccacttt tactagctgg agattttaac aacccagcag gtcaggaagg ttaccaagct
    21061 attttagcta gtccattagg cttacaagac gcatttgaag ttgctcaaga gaaaagtggt
    21121 agctatactg ttccgcctga aattgatggc tggaaaggga acactgaacc ccttcgaatc
    21181 gattatgtct ttactaccaa agagttagcg gtggaaaatt tacatgtcgt atttgatggt
    21241 aacaagagtc cacaagtgag tgatcactat ggcttgaatg ctatattaaa ctggaaataa
    21301 taactgaaaa gaggttggaa ctataaaatt ccagcctttt cttactagag aagctactgg
    21361 aaatagccta aataagtgag actactgtaa tggaataaaa tatggtataa ttgataaggt
    21421 agatagaatc gaggatgtta tgtcatttac gaaatttcaa tttaaaaact atattagaga
    21481 agccttgaag gagttaaaat ttacaactcc aacagaggtg caagacaagt tgattcctat
    21541 tgttttggca ggtcgtgacc tagtaggaga atcaaaaaca ggttcaggta agactcatac
    21601 tttcttgtta ccgattttcc agcaattaga tgaagctagc gatagtgtac aagcagtgat
    21661 tactgcaccg agtcgtgagt tggctactca aatttaccaa gtagcgcgtc agatttcagc
    21721 tcactcagat gtcgaagttc gtgtggttaa ttatgtgggt ggtacggata aggctcgcca
    21781 gattgagaaa ttggcaagca atcagcctca tattgttatt ggaacaccag gccgtatcta
    21841 cgacttggtt aaatctggtg atttagctat tcataaagcc aagacatttg ttgttgatga
    21901 agcagatatg accttggata tgggattctt ggaaactgtt gataagattg ctggcagtct
    21961 tccaaaagac ttgcaattca tggtcttctc agcgactatc ccacaaaaac tgcaaccatt
    22021 cttgaaaaaa tacttatcaa atcctgttat ggagaaaatt aagaccaaaa cggttatttc
    22081 tgacaccatt gataattggt tgatttcgac caagggacat gataagaatg ctcaaattta
    22141 ccagttgact cagttgatgc agccgtattt ggcaatgatt tttgttaaca ctaaaacgcg
    22201 tgctgatgaa ttgcattcat atctgactgc tcaaggcttg aaggttgcaa aaatccatgg
    22261 cgatattgcc cctcgtgaac gcaagcgaat catgaatcag gtgcaaaatc tggattttga
    22321 gtatattgtc gcaacagatt tggcagcgcg tgggattgac attgaaggtg tcagccatgt
    22381 catcaatgat gccattccgc aagacttatc tttttttgtt catcgtgttg gtcgtactgg
    22441 acgaaatggc ctaccaggta cagctattac cctttatcag ccaagtgatg actcggatat
    22501 ccgtgagttg gagaaattgg gaatcaagtt tagtcctaag atggtcaaag acggggaatt
    22561 tcaagatacc tatgaccgtg atcgtcgtgc caaccgtgag aaaaaacaag ataaacttga
    22621 tatcgaaatg attggtttgg ttaaaaagaa aaagaaaaaa gtcaaaccgg gttataagaa
    22681 gaaaattcaa tgggcggttg atgaaaagcg ccgtaaaacc aagcgtgctg aaaatcgcgc
    22741 tcgcggtcgt gcagagcgta aagctaaacg ccaaacattt taatagaaat tgttggagta
    22801 ttgagctcca actttttgct ttatgagaac gaactatcta aaccgaaaca ctacattaaa
    22861 gactgcaaat tgcgattaaa aatggtataa tgataaagtt atatagtccc gataagatgg
    22921 taggtattta ttacgaagag ttttcctatc agtactttgt aactctataa caatattttt
    22981 taagggggga catttttatg tcagagcgta aattattcac gtctgaatct gtatctgagg
    23041 ggcatccgga taagattgca gaccaaattt cagatgcgat tttggatgct attttagcaa
    23101 aggatccaga ggcgcacgtt gctgctgaaa cagctgtata tactggttct gtccacgttt
    23161 ttggtgaaat ttctacaaat gcctatgtgg atattaaccg tgtggttcgt gataccattg
    23221 cagagattgg ttataccaat acagaatatg gattttctgc tgagacggtg ggagtacacc
    23281 catctttggt ggaacaatct cctgacatcg ctcaaggtgt taacgaagcc ttggaggttc
    23341 gtggaaatgc tgatcaagat ccactggact tgattggagc aggtgaccaa gggctcatgt
    23401 ttggatttgc agtagatgaa acagaagagc ttataccatt gccaattgca ctcagtcata
    23461 aattggttcg tcgtctggca gaacttcgta agtctggaga aattagctat ctccgtccag
    23521 atgcaaaatc acaagttaca gttgagtacg atgaaaatga ccgtccggta cgtgtagata
    23581 cagtcgttat ttctactcag catgatccag aggccactaa tgaacaaatc catcaagatg
    23641 tgattgacaa ggtcatcaaa gaagttattc catcttctta tcttgatgat aagacaaaat
    23701 tctttatcaa tccgacaggt cgttttgtaa tcggtggtcc tcaaggggac tcaggtttga
    23761 ctggtcgtaa gattattgta gatacttatg gtggctactc tcgtcatggt ggtggtgcct
    23821 tctctggtaa agatgcgact aaggtggatc gttcagcctc ttatgcggct cgctatattg
    23881 ccaagaatat cgttgcagca gaccttgcta agaaggcaga agtgcagttg gcctatgcta
    23941 tcggtgttgc gcaacctgtt tctgttcgta tcgatacttt cggtacagga acagtagctg
    24001 aaagtcaact tgaaaaagcg gctcgtcaaa tctttgacct tcgccctgca gggattatcc
    24061 aaatgctgga cctcaagcgt ccaatttacc gtcaaacatc ggcttacggt cacatgggac
    24121 gtacagatat tgatcttcca tgggaacgtt tggataaggt agatgctttg aaagaagcag
    24181 taaaataaga ttttaagagg ggaacgtcct ctctttttta tagtttttaa ctatactggg
    24241 atactgttct gaaaatccat tttgcgaaag tagagattta
//
LOCUS       DAVKGC010000021        23792 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKGC010000021 DAVKGC010000000
VERSION     DAVKGC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23792
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.021"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(97..2355)
                     /locus_tag="VW049_000673"
     CDS             complement(97..2355)
                     /locus_tag="VW049_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9431836.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGFA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAIMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2584..2814)
                     /locus_tag="VW049_000674"
     CDS             complement(2584..2814)
                     /locus_tag="VW049_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9431837.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3004..3687
                     /locus_tag="VW049_000675"
     CDS             3004..3687
                     /locus_tag="VW049_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9431838.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQVGILGVDLSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KRMGQGDKK"
     gene            3687..4421
                     /locus_tag="VW049_000676"
     CDS             3687..4421
                     /locus_tag="VW049_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9431839.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESKAVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4575..5432
                     /locus_tag="VW049_000677"
     CDS             4575..5432
                     /locus_tag="VW049_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9431840.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTEKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGFRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLSKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5575..6447
                     /locus_tag="VW049_000678"
     CDS             5575..6447
                     /locus_tag="VW049_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9431841.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPDLHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLSKIMK
                     IICQQDRVSKDKLV"
     gene            6729..7412
                     /gene="rpiA"
                     /locus_tag="VW049_000679"
     CDS             6729..7412
                     /gene="rpiA"
                     /locus_tag="VW049_000679"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9431842.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7426..8637
                     /locus_tag="VW049_000680"
     CDS             7426..8637
                     /locus_tag="VW049_000680"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9431843.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8639..9181
                     /locus_tag="VW049_000681"
     CDS             8639..9181
                     /locus_tag="VW049_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU9431844.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVYYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9197..10006
                     /locus_tag="VW049_000682"
     CDS             9197..10006
                     /locus_tag="VW049_000682"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9431845.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10496..10891
                     /locus_tag="VW049_000683"
     CDS             10496..10891
                     /locus_tag="VW049_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEU9431846.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            10901..11476
                     /locus_tag="VW049_000684"
     CDS             10901..11476
                     /locus_tag="VW049_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9431847.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            11501..12013
                     /locus_tag="VW049_000685"
     CDS             11501..12013
                     /locus_tag="VW049_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9431848.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12669..13379
                     /gene="deoD"
                     /locus_tag="VW049_000686"
     CDS             12669..13379
                     /gene="deoD"
                     /locus_tag="VW049_000686"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9431849.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13402..13926)
                     /locus_tag="VW049_000687"
     CDS             complement(13402..13926)
                     /locus_tag="VW049_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9431850.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     HEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(13979..14215)
                     /locus_tag="VW049_000688"
     CDS             complement(13979..14215)
                     /locus_tag="VW049_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9431851.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(14283..15203)
                     /locus_tag="VW049_000689"
     CDS             complement(14283..15203)
                     /locus_tag="VW049_000689"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9431852.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15393..15798
                     /locus_tag="VW049_000690"
                     /pseudo
     CDS             15393..15798
                     /locus_tag="VW049_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16014..16595
                     /locus_tag="VW049_000691"
     CDS             16014..16595
                     /locus_tag="VW049_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9431853.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16592..17869
                     /locus_tag="VW049_000692"
     CDS             16592..17869
                     /locus_tag="VW049_000692"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9431854.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            17887..18549
                     /gene="deoC"
                     /locus_tag="VW049_000693"
     CDS             17887..18549
                     /gene="deoC"
                     /locus_tag="VW049_000693"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9431855.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18536..18925
                     /locus_tag="VW049_000694"
     CDS             18536..18925
                     /locus_tag="VW049_000694"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9431856.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19015..20067
                     /locus_tag="VW049_000695"
     CDS             19015..20067
                     /locus_tag="VW049_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9431857.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20211..21746
                     /locus_tag="VW049_000696"
     CDS             20211..21746
                     /locus_tag="VW049_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431858.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            21739..22797
                     /locus_tag="VW049_000697"
     CDS             21739..22797
                     /locus_tag="VW049_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9431859.1"
                     /translation="MFKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            22800..23756
                     /locus_tag="VW049_000698"
     CDS             22800..23756
                     /locus_tag="VW049_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9431860.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         6901 a         4309 c         5417 g         7165 t
ORIGIN      
        1 tcataatgga caaagggcaa gctgtcaaga aattacagtt attttagtaa aaaaagaagc
       61 agaaaaattt cattttctac tccttttcta taggatttat ttttcttttt cagaactttt
      121 aacttcagct tttttggcag atttaatctg aatgtttccc ttgctagtca taattgcttt
      181 gagatctttt tcgcttggat tttcaaggta gtagtcagtg attgtgtcct caatatagtc
      241 ttgaatagtc cgacgaagtg ggcgtgctcc catttttgga tcataaccta ggtcaaccaa
      301 cttttccttg accttgtcag ttacatccaa acgaatgttg ttgctagaga ggcgcttgtt
      361 aacatctgct agcatgagct cgacaatctg aaggaggtta tccttgctga gagccttaaa
      421 ttcgataatg ccatcaaaac ggttcataaa ctctgggcta aagaagttac cgagttcacc
      481 gaggacagaa ttggtacgtc cttctctagc agcaccaaat ccaacgctag cttcggtctt
      541 tcctgtacct gcatttgagg tcatgataat gatggcatcc ttgaagctaa cggtgcgtcc
      601 ttgcccgtct gtcaaacgac catcgtccaa gacttgaaga aacatgtgca taacatctgg
      661 gtgagctttt tccacttcat cgagaaggat gagagaatat ggattgtggc gaactttttc
      721 agttaattga ccagcctcat catagccaac ataacctgga ggggcgccga ccaacttagc
      781 cacactatgt ttttccatgt attcactcat atcaaagcga atcatactat cagcaaaacc
      841 aaaaagttcg atagccagtt gtttggaaag ttctgtctta ccgacaccag ttggcccaac
      901 gaagaggaag cttccgattg ggcggttagg ggtaccaagt ccgacacgat tacggcgaat
      961 agccttggca atcttatcga ctgcatcatc ttggccaata acatgagact tgagatcttc
     1021 ggctagatgg atgagttgag attgttcttt ctctttcaaa tcaccaacag ggatattggt
     1081 tttctgctcg ataatgtgct caatagtttt ctcgctgatg ataggagtat cctggtctgt
     1141 gatctttttc ttttgcattt ccttatactt ggcaatctgg tcgcggaagt aggccgcctt
     1201 ctcaaaatct tcttctcgtg tagcttgaga cttgagattt tcagcctcaa tcaagcgctg
     1261 atcaattact ttaggatcca caaaattcaa ggtcaagttc atcttagaac cagcttcatc
     1321 taggaggtca atggccttgt caggcaagaa gcgatcttgg atgtagcgat tggaaagagt
     1381 tgcagctgct tcaatcgcag catctgtata ttgaacgtgg tggtaatctt cgtatttctt
     1441 ttgaatccct ttgagaatag tgattgtttc gtccaccgtt ggttcatcga ctttaacagg
     1501 ctgcatacga cgctcgaggg cagcatcctt ttcaatgata cggtattcat tgagggtagt
     1561 agcaccgact agttgcagtt ctccacgagc aagggctggc ttgaggatat ttcctgcgtc
     1621 catattacca tcactcgcag aaccagcacc aacaatttca tggatttcat cgataaagag
     1681 gatgatgtct tcacgtttgc gaatttcttc catgagtttt tgcatgcgtt cttcaaattg
     1741 tcctcgaatc cccgttcctt gaactaagct aaccacatcc agacggatga cttgtttacc
     1801 ttggagttta tgtggcacat cgccatcgac aattttctga gctagacctt cgacaacggc
     1861 cgtttttccg acaccaggtt caccgataag gacaggatta ttcttggttc tacgattgag
     1921 aatctcgatg acacggataa tctcatcgtc gcgcccaata acggggtcaa tgtctccacg
     1981 acgggcaatt tcagttacat taataccaaa ttcttccagc aggccttttt cttggctagg
     2041 tggcggagtt tgagcagatc cacgattttg ggaaccataa ccgccgtttc caccgtaacc
     2101 tccacctgat tgggttgggg gaataggagg agtattgcta gaaggtctga aattgtttag
     2161 atcattgaag aaatcaccaa agggatcgaa gtcacgattg ttcagatccg tcataccttt
     2221 gaagaggcta ttgttaggat ctgttttgat aatcttatag cagttttgac agaggtcaat
     2281 ttgtttttgt tttccattga gattggtgta aagatgaatt gttgagtcgt taattttaca
     2341 gttttgacaa agcatacatc tacctcattt ctttctttag cctgacttgt ttaaaggtca
     2401 aaaatagtca aattctatac tctcattata acaggattgg ggtgtaaagc aagtaaaaag
     2461 attgaagctt tgagaagttg ttacaatgaa aatttttgtg aatttggact ataaaaaaat
     2521 cctatttgtg agacaaaaag gattttactt agacatgcag ggacaatccc agcttgtttt
     2581 agattagtag agaagttcgt aaatttccat tgcgattaag tcaatgctat caaacgtata
     2641 cgtttcgcga tcttctacgt cacgaagggt aaagattggg ccgttttctt cgtcgtggct
     2701 gtacgcaact tctgcaacaa caactccttc gcgttcaaaa ttacgtttta aatttccgcc
     2761 atcgtttgcc attgcttcaa ggcggttgat gattctaacc aaatgtgatt ccattttgat
     2821 tctccttcat ttcttatcgt tactatttta ccataaagga ggccaacttg actagaaaaa
     2881 actaagattt ctcgctaatt ctgctagttt aaagattttt aaataaattg gataaaaaat
     2941 ttaaatttta attcaaaaca tgttataatc atacagtatt ctattttaga aagcagtgtg
     3001 actatgaatt tttctttttt acctaagtat ttaccttatt ttaactatgg ggctgttgtg
     3061 acgattctta tttctatctg tgttatcttt ttgggaacta ttttgggtgt tgtcttggct
     3121 tttgggcaac gttcaaagtt taaaccgctt gtttggttgg ccaacttgta cgtttggatt
     3181 ttccgtggga caccgatgat ggttcaaatt atgattgcct ttgctcttat gcatatcaat
     3241 gctccgacta ttcaggttgg aattttaggt gttgatcttt ctcgtctgat tccagggatt
     3301 ttgattatct ctatgaacag tggtgcttat gtttctgaga ctgttcgtgc cggaatcaat
     3361 gcagttccta aggggcagtt agaggcggct tattctttag ggattcgtcc taaaaatgct
     3421 atgcgctatg taattttacc acaagctatc aaaaatatct tgccagcttt ggggaacgaa
     3481 tttatcacca ttatcaagga cagctccctc ttatcagcta tcggtgttat ggagttgtgg
     3541 aacggggcta caacagtttc tacaacaacc tatctacctt taacaccact tttatttgca
     3601 gctttttatt acttgattat gacttctatt ttgacagtag ccttgaaagc ttttgaaaaa
     3661 cgtatgggac aaggagataa gaaataatga cagaaacctt gataaaaatt gaaaatttac
     3721 ataaatcatt tggaaagaac gaagtattga agggcatcaa cctcgagatt aaaagaggag
     3781 aagttgtcgt tatcatcggt ccttcaggga gcgggaaatc taccttgctt cgctctatga
     3841 atttgttgga agaagcgacc aagggtaagg ttatctttga gggagtcgat attacggaca
     3901 agaagaatga cctctttgcc atgcgtgaga agatgggcat ggttttccaa caatttaacc
     3961 tctttcctaa tatgactgtg atggaaaata tcaccttgtc acctatcaag accaaaggtg
     4021 agagcaaggc tgttgctgag aagagagccc aagagctttt ggaaaaagtt ggtttgccag
     4081 ataaggcaga tgcttatcca cagagtttgt caggtggtca gcaacaacgg attgccattg
     4141 cgcgtgggtt ggccatggaa cctgatgttt tgctctttga cgagccaact tcagcactag
     4201 atcctgagat ggttggagaa gttctggctg ttatgcaaga tctagccaag tcaggaatga
     4261 ccatggttat cgtaacacat gagatgggat ttgcccgtga ggtggcagat cgtgtcatct
     4321 ttatggcaga cggtgtggtt gttgaagacg gaacaccaga acagattttt gaacaaaccc
     4381 aagaacaacg gactaaagac ttcttgagta aggttttata atctatttta aaatttcaag
     4441 acaagattag tgaaaagctc aaccagagct ttttcttata gtttgaaact ataggattgc
     4501 ctaggaaaga agtgttagag ggtgctggca gtttttggta taatggaaga tatttgaaag
     4561 aaaagagaag tgatatgaca cagattattg atggaaaagc tttagcggcc aaattgcagg
     4621 ggcagttggc tgaaaagact gaaaaattaa aggaagaaac aggtctagtg cctggtttgg
     4681 tagtgatttt ggttggggac aatccagcca gccaagtcta cgttcgcaac aaggagaggt
     4741 cagctcttgc agctggtttc cgtagcgaag tagtgcgagt tccagagacc attactcaag
     4801 aggaattgtt agacctgatt gctaaatata atcaggatcc agcttggcat gggattttgg
     4861 tccagttgcc attaccaaaa catatcgatg aagaggcggt tttattagcc attgacccag
     4921 aaaaggatgt ggatggtttc catcccctaa acatgggacg tctttggtct ggacatccag
     4981 tcatgattcc ctcgacacct gcaggaatta tggaaatgtt ccatgaatat gggattgact
     5041 tggaaggtaa aaatgcagtc gtcatcggtc gatccaatat tgtcggaaaa cctatggccc
     5101 agcttctttt ggcaaagaat gcaacagtaa ccttgactca ctcacgtact cataatcttt
     5161 ccaaggtggc tgcaaaagca gatattctgg ttgttgcaat cggccgcgcc aagtttgtga
     5221 ctgctgactt tgtcaaacca ggtgcggtag tcattgacgt tgggatgaac cgcgatgaaa
     5281 atggtaagct ctgtggggat gttgattatg aggcggttgc cccacttgct agccacatta
     5341 cgccagtccc tggaggtgtc ggtcctatga ccattactat gctgatggag caaacctatc
     5401 aggcagcact taggacattg gatagaaaat aagagaaaca ttcgattaat agagtgtatt
     5461 ttctatagct atatctaaca atggcagaaa tgaatattaa attttagata taagattaca
     5521 aaaggaggtc tgcgtctcct ttttattgta taataaaagt gagaggaaaa aaggatgaaa
     5581 gtgattaatc aaaccttatt agaaaaagtt attattgaac gttctcgttc aagtcataaa
     5641 ggagattatg gtcgtctgct gctgcttggt ggtacttatc cttatggagg tgccatcatc
     5701 atggctgctt tagcagctgt aaaaagcggt gcaggattgg tgaccgttgg aacggacagg
     5761 gaaaatatcc cggatctgca cagccattta cctgagccta tggccttttc tcttcaagac
     5821 cagcaattgt taaaagagca attggagaag gcagaagtta tcttgctggg gcctggttta
     5881 ggagacaatg cttttggaga agatttagtt aaacaggtct ttgctggctt aaaacagaat
     5941 cagattttga ttgtagacgg aggtgccttg accattcttg ctaggacaag tttgtcattt
     6001 ccctcaagcc agcttatcct aactcctcac caaaaagaat gggaaaaatt atctggaatt
     6061 acgattgaaa agcaaaagga agatgcaacg gctagtgttc tgacttcctt ccctcaagga
     6121 acaattttgg tggagaaagg cccagctact cgtatttggg aagttggcca atctgattat
     6181 taccagttac aggttggtgg tccttatcag gcgactgggg gaatgggaga tactctggct
     6241 gggatgatcg caggatttgt aggccaattc cgacaggcca gtctctacga gcgtgtggca
     6301 gtagcgaccc atcttcattc agccatagcc caagaactat ctcaagaaaa ttatgtggtc
     6361 ttaccgacgg aaatcagcag gtatctttcc aaaataatga aaattatttg tcaacaagat
     6421 agggtaagca aagataaact tgtttaaaat agacagataa atctacttcc ttctcttaat
     6481 ttatgattct tgaaatgcgc ctgctttaag gctaaactag acgtatcaaa gttaagagtt
     6541 tgtttttgta actggaaaga gatgaacaaa tcaaatacat aaaagataaa ctcattttta
     6601 ttcgtttggt aaaaataaaa tgcatatttt taaagaaaga ttgatggaaa atgtagattt
     6661 gatgttttta cttgaataaa aagcggtttc atagtaaaat aagaactgag aatgatggag
     6721 gtaaatcggt ggaaaatctg aagaaaatgg caggtatcaa ggctgctgag ttcgtgagcg
     6781 atggaatggt cgttggactt ggaacaggct cgactgccta ttattttgtc gaagaaatcg
     6841 gtcgtcgtat caaggaagaa ggattgcaga ttacagctgt aacaacttct agtgtgacca
     6901 ctaaacaggc agaaggcctt aatatcccgc tcaagtctat tgaccaagta gactttgtcg
     6961 atgtgacggt tgatggcgcg gatgaagtgg atagtcagtt taatggaatc aaaggcggtg
     7021 gtggtgccct tctcatggaa aaggtggtcg caacaccatc aaaagaatac atttgggtgg
     7081 tggatgaaag caagctggtc gaaaaactag gtgcttttaa attgccagta gaagtggttc
     7141 agtatggtgc agagcaggtc tttcgtcatt ttgaacgagc tggctacaaa ccaagtttcc
     7201 gtgaaaaaga cggccaacgt tttgtgacgg atatgcagaa ttttatcatt gacctcgcct
     7261 tggatgtcat tgaaaatcca attgcttttg gacaagaatt ggaccatgtc gttggtgttg
     7321 tggagcatgg tttattcaac caaatggtgg ataaggtaat cgttgctgga cgagatggag
     7381 ttcagatttc aacttcaaaa aaaggaaaat agaagggggc ataagatgtc taaatttaat
     7441 cgtattcatt tggtggtact ggattctgta ggaatcggtg cagcaccaga tgctaataac
     7501 tttgtcaatg caggggttcc agatggagct tctgacacac tgggacacat ttcaaaaaca
     7561 gttggtttga atgtcccaaa catggctaaa ataggtcttg gaaatattcc tcgtgaaact
     7621 cctcttaaga ctgtagcagc tgaaagcaat ccaactggat atgcaacaaa attagaggaa
     7681 gtatctcttg gtaaggatac tatgactgga cactgggaaa tcatgggact caacattact
     7741 gagcctttcg atactttctg gaacggattc ccagaagaaa tcctgacaaa aatcgaagaa
     7801 ttctcaggac gcaaggttat tcgtgaagcc aacaaacctt attcaggaac ggctgttatc
     7861 gatgattttg gaccacgtca gatggaaact ggagagttga ttatctatac ttcagctgac
     7921 cctgttttgc agattgctgc ccacgaagac attattcctt tggatgaatt gtaccgtatc
     7981 tgtgaatacg ctcgttcgat tacccttgag cgtcctgccc ttcttggtcg catcattgct
     8041 cgcccttatg taggtgaacc aggtaacttc actcgtacgg caaaccgtcg tgacttggct
     8101 gtatctccat tttccccaac tgttttggat aaattgaatg aggctggtat cgatacttat
     8161 gctgtgggta aaatcaacga tatctttaac ggtgctggta tcaaccatga catgggtcac
     8221 aacaagtcaa atagtcatgg aattgataca ctattgaaga ctatgggact tgctgagttt
     8281 gaaaaaggat tctcattcac aaacctagtt gactttgatg ccctttacgg ccatcgtcgt
     8341 aatgctcacg gttaccgtga ttgcttgcat gagtttgatg aacgcttacc tgaaattatc
     8401 gcagctatga gagagaatga ccttctcttg attactgcgg accatggaaa tgacccaacg
     8461 tatgcaggaa cggatcacac tcgggaatat attccattgt tggcctatag ccctgccttt
     8521 aaaggaaatg gtctcattcc agtaggacat tttgcagata tttcagcgac tgttgccgat
     8581 aactttggtg tggaaactgc tatgattggg gaaagtttct tagataaatt ggtataagat
     8641 gacgcgctat gctttgctgg tgagaggtat caatgttggt ggtaagaata aggtcgtcat
     8701 ggcggagctt cgtcaagaat tgacaaactt gggactggaa aaggttgaga gctacatcaa
     8761 cagtggcaat attttcttta cttcgataga ttccaaagcc caattggttg aaaagctaga
     8821 gactttcttt gcagtctatt atccatttat tcagagcttt tctttactga gtctagagga
     8881 ctttgaggcg gaacttgaaa atctaccagc ttggtggagc agagacttgg cacgaaaaga
     8941 ttttctcttt tacactgagg gtttggatgt ggaccaagtc atcgcgacag ttgaaagttt
     9001 agagctgaaa gatgaagtgc tttattttgg aaaacttggg attttctggg ggaaattttc
     9061 tgaagaatcc tattctaaga ctgcctatca taagtacttg ctgaaggtgc ctttctaccg
     9121 ccacattact attcgtaatg ctaaaacctt tgacaaaatt ggtcaaatgc taaaaaaata
     9181 ataaaggaga cacacaatga catttttaaa caaaatccat gaaactgcta ctttcctgaa
     9241 agaaaaggga attgcagccc ctgagttcgg tctaatcctt ggatcaggac ttggagaatt
     9301 ggcagaagaa atcgaaaatc cagttgtagt agactatgct gagattccaa actggggccg
     9361 ttcaacagta gtcggtcatg ctggtaaatt ggtatatggt gaactggcag gtcgcaaggt
     9421 cttggctctt caagggcgtt tccatttcta tgaagggaat cctctggaag tggtgacttt
     9481 cccagttcgt gtgatgaaag ttcttggatg tgaaggtgtt attgtaacca atgcagctgg
     9541 cggtatcgga tttggtcctg gtaccttgat ggctatctca gaccatatca acatgacggg
     9601 gcaaaatcca ttgatgggtg aaaacttgga tgactttggc ccacgtttcc cagatatgtc
     9661 tagggcttac acaccagaat accgtgccac tgcccatgaa gtggctaaaa aacttaatat
     9721 caagcttgat gaaggtgtct atatcggagt tactggtccg acttatgaaa caccagcaga
     9781 aattcgttcc tataagacac tgggagcaga tgcagttggt atgtctacgg ttcctgaagt
     9841 tatcgtggca gcccactctg gcttgaaagt tctgggaatt tcatgtatca ctaactttgc
     9901 ggccggtttc caagaagaac tcaatcacga agaagttgta gaagtgactg aacgtgttaa
     9961 aggtgatttc aaaggcttgc ttaaagcgat tcttgctgaa ttgtaagaaa aaagatttaa
    10021 aagggggagt gcctctgtct tttcaggatt gactgcctat ccgtattaaa gaagaaacag
    10081 aggaatacta tgagcttctt cctgctctta taactgaaag aagcggaaga ataggtatgt
    10141 ctgatctgat agccagcatt gtgaaggaca agattctagg atactagcat tagcttccta
    10201 gcattagctt cctagccaag cagactagta tgataaggag agatgagaat gaattgactt
    10261 tctgaatttc tcagtcttat catatatagc acaatgagat ttcgcttgag tctgcttgta
    10321 aataaacgaa aagaaagata agaaataatg aaaattggtc aacgaattat gcgctttggc
    10381 ataaaaaatt aagtatcgga gttgtatctg ttgtagtcgg ctttgatttc tagctccagc
    10441 tggaatttca gccaatgaag taaagcaaga tgtaacatct gaagtggtaa taggtgtgct
    10501 agattctaag gaggaattga aagagtcaga aaatgatgct ccaaaactag aaactcctct
    10561 tagagaggag ccaagactag ctcctcaaac gcttccggaa gcaagtgaag ttcttgaaaa
    10621 caaaagggaa gagtcaaaag tagagataac agagccagct caagcggatg atatccgcaa
    10681 ggttgttggg gaattagcca aggatataag tattactaag ttgtatatga caggtcattc
    10741 tcttggaggc tacctagctc agattgcagc ggttgaagat taccaaaaat atcctgattt
    10801 ttataaccat gtattgagga aagtgacaac tttcagtgct cctaaagtga ttacttccag
    10861 aactgtttgg gatgctaaga atggtttctg agatgttggt ttggaaagtc gtaaattagc
    10921 tgttagtgga aaaattaagc attatgtggt tgataatgac aatgttgtga ctcccttgat
    10981 tcataataat cgtgatattg ttacatttac aggtaattca cgctttaaac accgttctcg
    11041 tggctatttt gaaagtccaa tgaatgatat tcctaacttt aatattggta aacaagctac
    11101 cttggataaa catggttatc gtgatccgaa attggataaa gtgcgattct ttaagaaaca
    11161 ggctctacct caatcttcta gtcaaccaag cgctgaacca atggaaaata ttgccttagg
    11221 aaaacaggtt actcaaagtt cgacagcttt cggaggagat gctagaagag ctgtggatgg
    11281 caaagtcgat ggtaactatg gtcacaattc tgtcactcat acaaacttcc aatctaagcc
    11341 ttggtggcaa gtagatttgg ctaaagaaga aaccattcgc caaatcaata tttacaaccg
    11401 aacagacact gcccaggata gattggcaaa ctttgatgtc attcttttag acagttctgg
    11461 taaagaaatt gagtgaaaac gtataatatc tcctaaagat gtgtcagcac aaattacgat
    11521 taaccataaa aaagcgcgct atgttcggat tgagctagaa ggctataatg ccctcagtct
    11581 tgcagaagtt gaagttttcc gctttatagc tacgaatgct gaaacggcga cacaagtttc
    11641 taagccagtt caaccaatca gtcagactcc tgtgaaagat aaaacattga caattcaaca
    11701 cagtggagct tacattgccc gctactccat aacttgggaa gaagttccag tagataaaga
    11761 tggaaaccaa gttgttcgta gtcattcttg ggaaggaaac ggttgcaacc agactgcagg
    11821 ttttgtcctc aacctcccaa tcaaagaaaa tatgagaaat ctgcgagtta agattgagaa
    11881 aaagacgggc ctactatgga atagatggca aacaatctat gaaaacagac caattttagc
    11941 tcaaccccac cgtaaaatta cccattgggg tacgacattg aattccaagg tgagtgacga
    12001 tgatgtcttg taatctgatg gtacaatgac agttagtttg tctagtttat aagaaagtac
    12061 tacctgagct tgaatagaac tcaggtagct ctatgaaaga acaaaattaa tactcaatga
    12121 aaatcaaaga gcaaactagg aaactagccg caggttgctc aaagcactgc tttgaggttg
    12181 tagataagac tgacgaagtc agtcacatat ataatccaag gcgacgttga cgtggtttga
    12241 agagattttc gaagagtata aacagaaagg tagagcgcgt gttctaattt gaacacgagt
    12301 agaaaacttt tctaaaaaca aaaacgaaag gatgggtaaa ctgtattcgc tgaactgaat
    12361 acgggcgact ctcctctaaa tcaaaattaa gaaagaaagg aattgacccc accctaaaag
    12421 cagtgggaaa aagatagttg atctagcgag catcgctcac tgcgcccaac tcctattttc
    12481 ccttcgcttt ttgatgggtt tggtatcttt ctcaatataa aatataaaat aaagaaaggt
    12541 agagcgtgtg ttttgatttg aacacgagcg gaaaactcgg aaaatagata atctgactga
    12601 aaaatcagga tttctcgtca ggttcctaat tttcagtcgt tttcttctcg ctctttgtat
    12661 cataaattat gtctatccat attgctgctc agcagggtga aattgctgat aaaattcttc
    12721 ttcctgggga tcctcttcgt gctaagttta ttgcggagaa tttccttgat gatgctgttt
    12781 gttttaacga agtgcgtaac atgtttggtt acactggtac ttacaagggt cactgtgtat
    12841 ctgtcatggg aactgggatg ggaatgccat ctatttcgat ttatgcgcgt gagttaatcg
    12901 tagactacgg tgtgaagaaa ttgattcgtg tgggaactgc aggttctttg aatgaagagg
    12961 ttcatgttcg tgaattagtt ttggcgcagg cggctgcaac caactcaaac atcgttcgta
    13021 atgactggcc acagtacgat tttccacaaa ttgctagctt tgatttgctt gataaagcct
    13081 accatatcgc caaaaaactt ggtatgacta ctcacgttgg gaacgttttg tcatctgatg
    13141 tcttttactc aaattacttt gaaaagaata tcgagcttgg taaatgggga gtcaaggctg
    13201 tggaaatgga agcagcagct ctttactatc ttgctgccca ataccatgtt gatgcgctag
    13261 ctatcatgac catctctgat agcttggtca atccagacga agacacaact gcagaagaac
    13321 gtcaaaatac cttcactgat atgatgaagg ttggtttgga aaccttgatt gcagaataat
    13381 tatatccaaa aaggaagccc tttatgaatt gtaggacttc cttttcttat ccagaaattg
    13441 atctagctct ctctgatttc gaagaatagt gactttatgt gaatattctt ggcaaagttt
    13501 ttggtaattt tctttttgag ttttgctacg tccatcccaa agaatccatc tgataaactc
    13561 ccagtcaaag cgttcagggc aatctgccgc catactttct ctgacttttc cacggtattt
    13621 aagataacgc ttaaaggctc taaagagaca ggtcaatggc gaaaaattga gaaagatgat
    13681 ttggtcagct tcttgcattc gttcttggta gtagcaccaa gaataattac catcgatgac
    13741 ccaagcttta tgcttggtga gaaagttttt tatctcggtt aacatccatt cgtggtcact
    13801 gtcttgccaa ccaggttgaa attggagtgt gtccatatgc agttttggaa tggagtagta
    13861 gttagataac ttttctgcta gagttgactt accagaacca gaatatccga taattgcgat
    13921 tttcattttc taccttttcc tatttggaga caaaaaaaca gcctctatgg actgtttctt
    13981 atttagcaag tttagctgaa agacgagctt tatcgcggct tgctttgttt ttgtgaatca
    14041 aacctttagt ttctgcttta tcgatagctg agctagcagc acggaaaagt tcttcagatg
    14101 ggtttgcttc gaaagctttt atagcagtac gcatagctga tttttgagct gagttctttt
    14161 cgttttgttt aacgttcaat tcagcgcgtt tgatagctga tttaatgttt gccaatgttc
    14221 ttacctccat atttactaac tataccatta tatatgaaaa cttacgtttt gacaagggaa
    14281 aattactttt ttaagtaaat ttcatcgatt tcatggttct tgcttttatg aagaatcact
    14341 tctgcacgat ttctggttgg ttcaatataa ttttgcagat ttgtgagatt gatactgatc
    14401 cagacctgat gggcaaagga ctccacttcc ccaatcggca tctgagtaaa acgataatag
    14461 tagctatcag ggtcgttttg ggctaggctc agcattttca agaaacggtc cagataccaa
    14521 ctttcaatat catccactcc agcatctaca tagatggaaa agtcaaagaa gtcagtgata
    14581 tagagacgat cgttttgtgg attttgaaag acattaattc cctcaacaat tacaaaatca
    14641 gcagctttga cactttgttt cttttcgggt acgatgtcgt aaacttcatg agaatagaca
    14701 ggaatatcta catcttgtcc atttttgatg cggtccaaga agttgagaag agcttccata
    14761 tcatagcttt caggaaatcc tttacgattt aaaatcccct gctcaatcaa ggtttgattg
    14821 ggatagagaa aaccatcagt tgtaaccaac tcaaccgtag catctgtaaa cgtacgggac
    14881 agtaggattt gaagtaggcg actggttgtg gattttccaa cggcaacact cccagaaacc
    14941 ccaataataa aaggttgaga tttactttca cgttggagga aaattccttt tgaaaaggct
    15001 aaatcttcct tagttcgctt gtaaatctga atcagatgag ccaaggggag atagatatct
    15061 gtaacgtctt ggagactgat ttggtcatta aaactcttga tagattccaa ttcttcttct
    15121 gtcaaaggag gtgttgtctt tcgatgtaaa gattgccaag tctggcggct gattttttca
    15181 aaatgtaaaa attcgttggt catttgtttt cccctaggta ttttgtctat cataccataa
    15241 aaagctaaaa aaataaagcg gtttcttgaa tgtggtaagt caatgatata taatataagc
    15301 gatcaattta ttgatgaaat aggagataga ttctactgat ttataagctt ataaaggggg
    15361 ctttttagtt cacaacttat aggaggtgta ctatgaaaat cttaaaacgt tacatattgg
    15421 aactctgttt tattttaagt tttgctttac cttttataaa aggagccaat gcagataatg
    15481 gtagatgctt tgtggaaacc tattacggtt ttactttttt gatggaacat gctattgtaa
    15541 cagctgtctt tatctgttcg tttttaattg ctttcttact aaaaaaaacg atgggcgaaa
    15601 tggattgctg ctggtagtta ttgcttttta gtcctatgga ttgcgacaga gggttatttc
    15661 ttccgcatgt cactagaaga tttgatacga ctttggacaa gtttggaaat cctgacacaa
    15721 acgtatcagt tgggctttta tctaaacatt ttgttgggaa ttttgttgat aataaagtat
    15781 tttaaggtta agcaatagaa ataataatct gcctgattat agatgataaa ctttttgttt
    15841 ttatctggac tgtgatattt atagttctaa atggtttatt atttataagt gttgaaagag
    15901 attgaagaaa atggcttcgt gactcgcttt cttcggtctt tttcgtttta ttatcaattt
    15961 gtaaacgatt tacaatttga gtcaaattat ggtaaaatag tgtcatgagt aaaatgtatt
    16021 atgcagaaaa tcctgacgct gctcacgaca ttcatgagtt gagagtagag ttgttgggac
    16081 aaaaaatgac ctttttaacg gatgcgggtg tttttagcaa gaaaatagtt gactttggaa
    16141 gtcaactatt gctcaagtgt cttgaggtca accaaggaga aactgtcctt gatgtaggct
    16201 gtggttacgg acctttgggt ttgtccttag ctaaggctta tggagttcag gctactatgg
    16261 ttgatatcaa cactcgtgcc ttggatttag ctcggagaaa tgctgaaaaa aataatgcaa
    16321 aagcgacgat tttccagtcc aacatctatg aacaagttga gggacatttt gatcatgtca
    16381 tttccaatcc tcctatccgt gcgggcaagc aagtcgttca tgaaatcatt gagaaaagta
    16441 aagatttctt ggaaactggt ggggatttga caatcgtcat ccagaaaaaa caaggggctc
    16501 caagtgctaa gtccaagatg gaggatgtgt ttggtaattg tgaaatcctt aaaaaagaca
    16561 aaggatacta tatccttaga agtgtgaaag aatgagagca gttgatttaa tacaaaaaaa
    16621 acgagacggt caagaactga cttcaagtga aattgaatgg ctggtagaag gctatgtgtc
    16681 aggaactgtt cctgattatc agatgtctgc ctttgctatg gctgtttatt ttaaaggaat
    16741 gacgactcgt gaaatttctg atttaacgat gaatatggtt aagactgggc aagagtttga
    16801 tctgtcagcc attgatggtg tgaaagttga caagcattct actggtggtg tcggcgataa
    16861 ggtaaccttg attttagctc ctcttgttgc tagcttcggt gtgcctgtag caaaaatgag
    16921 tggtcgtggt cttggccata caggcggaac aattgataaa ttggagtcca ttaagggcta
    16981 tcaagtagaa cgtagtcaag aggattttat tcgtcaggtt caggatattg gagtatctgt
    17041 cattgggcaa tcagaccagc tggtcaaagc agataagctt ctctatgccc ttcgtgatgt
    17101 gaccgcaact gtcgacacga ttcctttgat tgcgagttcg gtgatgagca agaaaattgc
    17161 ggcaggggcg gatgctattt tgctagacgt gactgtcggt gagggtgcct tcatgaagac
    17221 ggttgatgag gcgcgtgagt tggctcaaac tatggtagat cttggtaagg ttgttggacg
    17281 gaagacagta gcagtgatta cggatatgag ccagcctttg ggacgagcga ttggaaatcg
    17341 tctggaaatc cttgaagcat tggagatttt gcaaggtcaa ggacgtcagg atattaccca
    17401 ctttatttgt gaattggctc aaattatgct tggtttggca aatgttaaca aaactgttga
    17461 agaagttcgc caacatcttg agaatggtca agcactggct aagtttgagg aaatggtgca
    17521 agcccaaggt ggagacttgg aagacctcta tcgtcctgta aatgtagctc atgtggtgga
    17581 aatccctgct caggaaacgg gtgtcatttc agctcttcca gctatggact ttggtcttta
    17641 tgccatgagg ctgggtgctg gtcgtgcagt taagtctgat gccttggact atgaaacagg
    17701 aattgttttt gaaaagaaga ttggagactc cgttcaaaag ggtgaaattg ttgcaaaagt
    17761 atacacaaat gaaaaaattc cacctcaact agttacagat ttccaaaaat gtgttaaaat
    17821 aagtgatgaa gtgaaaaaaa tccgtgaaat tgtagaaatc atctcctaaa tggagggaaa
    17881 accgaaatga aattaaataa atatatagat catacgcttt taaaacaaga tgcaaagaaa
    17941 aaacaaattg atagtttgtt gtctgaggct agagagtatg actttgccag tgtttgcgtt
    18001 aatccgacct gggttgaaca tgctaaaaaa ggacttgaag gcacagatgt taaggtttgc
    18061 acagtagtag gtttcccctt gggagcaaca acttcagccg tgaaagcatt tgagacaaaa
    18121 gaagctatcc aaaatggtgc agatgagatt gatatggtga tcaatgttgg agctctcaaa
    18181 tcaggtaatt tagccttggt tgagtcagat attcgcgcag tagtggaagc aagtggtgat
    18241 aagttagtga aagtcattat tgaagcttgc cttctgacag accaagaaaa aattgttgtt
    18301 tgccaattgg cccaaaaagc tggggctgac tttgttaaaa catctactgg tttttcaact
    18361 ggtggtgcta cgatagcaga tgttacatta atgcgtgaaa cagttggatc tgatatggga
    18421 gtcaaggctg ctggtggagc tcgttcttat gcagatgctc ttgcctttgt cgaagcaggt
    18481 gcgacccgta tcggaacatc agctggggta gctattttaa aaggagaatt ggcagatggc
    18541 gactactgag ttgattgaac tggcaattga aaccagcaaa catgcctatg tcccctattc
    18601 tcactttcct atcggagcag ttttagtagc caaagacgga agtgtttaca cgggagtgaa
    18661 tatcgaaaat gctagctatc ctttgaccaa ttgcggtgag agaacagcta tttttaaggc
    18721 tatatctgaa ggccaaagag aattttcaga attgattgtc tatggtcaaa ctgaaaaacc
    18781 gatctcgcca tgtggtgctt gtcgccaagt gatggctgaa ttttttgaac aagatctaaa
    18841 agtgacctta gtcgcaaaag ataaatcgac ggtcgagatg acggtcgggg aattacttcc
    18901 atactctttt acagacttaa actagtctga gtcgctctct gagtggcacg gtccttgtgg
    18961 ccaaccaatc catacttgca acatcgttgc acatcttatt taggaggttc agtaatgaac
    19021 aagaaacaat ggctaggtct tggcctagtt gcagtggcag cagttggact tgctgcatgt
    19081 ggtaaccgct cttctcgtaa cgcagcttca tcttctgatg tgaagacaaa agcagcaatc
    19141 gtcactgata ctggtggtgt tgatgacaaa tcattcaacc aatcagcttg ggaaggtttg
    19201 caggcttggg gtaaagaaca caatctttca aaagataacg gtttcactta cttccaatca
    19261 acaagtgaag ctgactacgc taacaacttg caacaagcgg ctggaagtta caacctaatc
    19321 ttcggtgttg gttttgccct taataatgca gttaaagatg cagcaaaaga acacactgac
    19381 ttgaactatg tcttgattga tgatgtgatt aaagaccaaa agaatgttgc gagcgtaact
    19441 ttcgctgata atgagtcagg ttaccttgca ggtgtggctg cagcaaaaac aactaagaca
    19501 aaacaagttg gttttgtagg tggtatcgaa tctgaagtta tctctcgttt tgaagcagga
    19561 ttcaaggctg gtgttgcgtc agtagaccca tctatcaaag tccaagttga ctacgctggt
    19621 tcatttggtg atgcggctaa aggtaaaaca attgcagccg cacaatacgc agccggtgca
    19681 gatattgttt accaagtagc tggtggtaca ggtgcaggtg tctttgcaga ggcaaaatct
    19741 ctcaacgaaa gccgtcctga aaatgaaaaa gtttgggtta tcggtgttga tcgtgaccaa
    19801 gaagcagaag gtaaatacac ttctaaagat ggcaaagaat caaactttgt tcttgtatct
    19861 actttgaaac aagttggtac aactgtaaaa gatatttcta acaaggcaga aaaaggagaa
    19921 ttccctggcg gtcaagtgat cgtttactca ttgaaggata aaggggttga cttggcagta
    19981 acaaaccttt cagaagaagg taaaaaagct gtcgaagatg caaaagctaa aatccttgat
    20041 ggaagcgtaa aagttcctga aaaataatgg atggaagtca tttcttagga agcggccaag
    20101 ggccgctttt ttaagagttg agacaaagtc attttgtctc agtaaagctt gctactagac
    20161 aaactagcaa gttttattga aataaaataa gactctgaaa ggaagagcac atggcacacg
    20221 aaaatgtcat tgagatgcgt gatattacca aggtgtttgg tggatttgtt gccaacgaca
    20281 aaatcaactt gcacctacga aaaggtgaaa ttcatgcact tttaggagaa aatggggctg
    20341 gtaagtccac gctaatgaac atgttagcag gccttcttga accaactagt ggtgaaatcg
    20401 cggtcaacgg tcaagttgtc aatctcgact ccccatctaa agcagctagc ttgggaatcg
    20461 ggatggttca ccagcacttt atgttggttg aagccttcac agtggctgaa aacatcattt
    20521 taggtagtga attgactaaa aatggtgtgc tagatatcgc tggagctagc aaagaaatca
    20581 aggctctttc tgaacgttat ggcttagctg ttgacccttc tgccaaggta gcagatatct
    20641 cagttggagc ccaacaacgt gtagaaattt taaaaacact ttatcggggg gctgatatcc
    20701 ttatctttga cgaaccaacg gctgttttga ctccatcaga aattgatgag ttgatggcta
    20761 ttatgaaaaa tcttgtcaaa gaaggaaaat caattatctt gattacccac aaattggatg
    20821 aaattcgcgc agtttctgac cgtgttacag ttatccgtcg tgggaaatca attgaaaccg
    20881 ttgaaattgc aggggctact aatgctgatt tggcggaaat gatggtagga cgttctgttt
    20941 cctttaaaac agagaagcaa gcctctaaac caaaagaagt ggttttgtct atcaaagatt
    21001 tggtggtcaa tgaaaaccgt ggtgttccag ctgttaaaaa tctgtccttg gatgttcgtg
    21061 ctggagagat tgttggtatt gcggggattg atggaaatgg tcagtctgaa ctgattcaag
    21121 ccattacagg tcttcgtaag gttgaatctg gtagcattga gctaaaaggg gattcaattg
    21181 taggcttgca cccacgtcag attacagaac taagtgttgg gcacgttcca gaagaccgtc
    21241 accgtgatgg cttgattttg gaaatgatga tatctgaaaa tattgccctt caaacctact
    21301 ataaagaacc acatagtaaa aatggaattt tgaattattc aaatattact tcttatgcta
    21361 aaaagctgat ggaagagttt gatgttcgcg ctgccagtga attagttcct gcagctgcac
    21421 tctcaggagg aaatcaacaa aaagcaatta ttgctcgtga aattgatcga gatcctgatc
    21481 tccttatcgt tagccagcca actcgtgggt tggatgtcgg tgccattgag tatatccaca
    21541 aacgcttgat tgaagagcgt gataatggca aggctgtcct tgttgtcagc tttgaattgg
    21601 atgagatttt aaacgtctca gaccgtattg ccgttatcca cgatggtaag attcaaggta
    21661 ttgtatcacc agaaacaacc aataaacaag aacttggtgt cttgatggct ggtggaaact
    21721 tgggaaagga gaagagtgat gtttaaaaaa ttacaacaaa tttcggttcc cttgatttct
    21781 gtatttctag gaattttact cggagccatt gtcatgtgga tcttcggtta tgatgctatt
    21841 tggggctacg aagaattgtt ctatacagcc tttggcagtc tgcgtgggat tggagaaatc
    21901 ttccgtgcta tgggtcctct ggtcttgatt ggtcttggtt ttgcggttgc cagtcgagct
    21961 ggtttcttta acgtcggact tcctggtcag gctttggcag gttggattct cagtggttgg
    22021 tttgcgctgt cgcatccaga tatgccccgt cccttgatga ttctagcaac catcgtgatt
    22081 gccttgattg ctggtgggat tgtcggagcg attccaggta ttcttagggc ctatctaggg
    22141 acgtcagagg ttattgtaac catcatgatg aactacattg tcttgtatgt agggaatgcc
    22201 tttatccatg ctttccctaa agacttcatg caaagtacag attcgaccat tcgtgttggg
    22261 gctaatgcaa cctatcagac accttggttg gctgagttga ctggtaactc acggatgaat
    22321 attggtattt tctttgccat cattgccgtt gcagttattt ggttcatgct caagaaaaca
    22381 actcttggtt ttgaaatccg tgcagttggt cttaatccac atgcttcaga atatgctggt
    22441 atttctgcca agcggactat tatcctatct atgattattt caggtgcctt ggcaggtctt
    22501 ggtggagctg ttgaaggttt gggaaccttc cagaacgtct atgttcaagg ttcgtcatta
    22561 gctatcggat ttaacggaat ggcggttagt ttgcttgcgg ccaactcacc aattggtata
    22621 ctctttgcag ccttcctatt tggcgttctc caagttgggg ctcctggtat gaatgcggcg
    22681 caggtaccat ctgagcttgt cagcattgta acagcgtcta ttatcttctt tgtcagtgtt
    22741 cattacctta tcgaacgctt tgttaaaccg aaaaaacaag ttaaaggagg taagtaaaga
    22801 tgtctattat aaccttgctc cccctcttgg tgtcttctat gctgatttac tcagcacctc
    22861 tcatctttac aagtatcggt ggtgttttct ctgaacgtgg tggtgtggta aacgttggcc
    22921 ttgaaggaat tatggttatg ggtgcctttt ctggagttgt ctttaacctt gaatttgcag
    22981 aacaatttgg agcagcaact ccatggctat ccttgcttgt agcaggattg gttggtagtg
    23041 ttttctctat catccacgca gcagcgacgg ttcatttccg tgcagaccat gttgtcagcg
    23101 gtacggtatt gaacttgatg gcgcctgcct tggctgtttt cttagttaaa gttctttata
    23161 acaaaggaca aaccgacaac ctaagtcaaa cttttggacg ctttgatttc ccagtcttgg
    23221 caaatatccc agtgattggt gatatcttct tcaagtcaac tagtctactt ggttatctgg
    23281 cgattgcctt ctcattcctt gcttggttta ttctctttaa gactcaattt ggtcttcgtc
    23341 tccgctctgt cggtgaacat cctcaagcag cggatacctt gggaatcaac gtctacaaga
    23401 tgagatattt agggattatt atttcaggtt ttctaggtgg aattggcgga gcgatttatg
    23461 cgcaatcaat ctcagttaac ttctcagtga caactattgt tggacctgga tttatcgccc
    23521 ttgctgcgat gatctttggg aaatggaatc caatcggtgc tatgctttct agtctcttct
    23581 ttggactttc acaaagtttg gctgttatcg gttctcaatt gccgttccta caaggagtgc
    23641 cagcggttta tcttcaaatt gcaccttatg ttttgacaat tcttgtctta gcagccttct
    23701 ttggaaaagc agtcgcacca aaagcagatg gtatcaacta catcaaatca aaataagcat
    23761 acaaaaaaac gtcagttcaa tgactgaact gc
//
LOCUS       DAVKGC010000022        23771 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKGC010000022 DAVKGC010000000
VERSION     DAVKGC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23771
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.022"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            308..2461
                     /locus_tag="VW049_000699"
     CDS             308..2461
                     /locus_tag="VW049_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9431861.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2474..3823
                     /locus_tag="VW049_000700"
     CDS             2474..3823
                     /locus_tag="VW049_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9431862.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            3993..4700
                     /locus_tag="VW049_000701"
     CDS             3993..4700
                     /locus_tag="VW049_000701"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9431863.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            4757..8482
                     /locus_tag="VW049_000702"
     CDS             4757..8482
                     /locus_tag="VW049_000702"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9431864.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDINGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAVGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8575..10017
                     /locus_tag="VW049_000703"
     CDS             8575..10017
                     /locus_tag="VW049_000703"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9431865.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGTGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSMTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10054..11076
                     /locus_tag="VW049_000704"
     CDS             10054..11076
                     /locus_tag="VW049_000704"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9431866.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLVSSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11073..11618
                     /gene="purN"
                     /locus_tag="VW049_000705"
     CDS             11073..11618
                     /gene="purN"
                     /locus_tag="VW049_000705"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9431867.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11702..12211
                     /locus_tag="VW049_000706"
     CDS             11702..12211
                     /locus_tag="VW049_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9431868.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12236..13783
                     /gene="purH"
                     /locus_tag="VW049_000707"
     CDS             12236..13783
                     /gene="purH"
                     /locus_tag="VW049_000707"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9431869.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSAN
                     GETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIAVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            13905..15167
                     /gene="purD"
                     /locus_tag="VW049_000708"
     CDS             13905..15167
                     /gene="purD"
                     /locus_tag="VW049_000708"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9431870.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIENHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPMAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIKGLFYRTDIGSKAIK"
     gene            15570..16058
                     /gene="purE"
                     /locus_tag="VW049_000709"
     CDS             15570..16058
                     /gene="purE"
                     /locus_tag="VW049_000709"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9431871.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVISAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDRSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16045..17136
                     /gene="purK"
                     /locus_tag="VW049_000710"
     CDS             16045..17136
                     /gene="purK"
                     /locus_tag="VW049_000710"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9431872.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17146..17373
                     /locus_tag="VW049_000711"
     CDS             17146..17373
                     /locus_tag="VW049_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU9431873.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17436..18734
                     /gene="purB"
                     /locus_tag="VW049_000712"
     CDS             17436..18734
                     /gene="purB"
                     /locus_tag="VW049_000712"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9431874.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(18789..22673)
                     /gene="strH"
                     /locus_tag="VW049_000713"
     CDS             complement(18789..22673)
                     /gene="strH"
                     /locus_tag="VW049_000713"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9431875.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKDKPAKKDEAKAEADKPATEAGKERAA
                     TVSEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTDLHLLVGNDGLRFMLDDMS
                     ITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTDLINYAKDKGIGLIPTVNS
                     PGHMDAILNAMKELGIQNPNFSYFGKESARTVDLDNEQAVAFTKALIDKYAAYFAKKT
                     EIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYEKFIAYANDLARIVKSHGL
                     KPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVASSKLLAEKGHQILNTNDAW
                     YYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIPIIGGMVAAWADTPSARYS
                     PSHLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRYTAESVAAVKEAEKAIRSL
                     DSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKREVEKLAKNKVISIDAGRK
                     YFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTITANGKTYASDDVKKAIIE
                     GTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSPGHMDAMLVAMEKLGIKNP
                     QAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKIFNFGTDEYANDATSAQGW
                     YYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYEDKDDVQFDKDVLISYWSK
                     GWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGGFLKKAIENTGKTPFNQLA
                     STKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFADHNKDYFRANYNALREEL
                     AKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTLVANLKAALQGLKPAATHS
                     GSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAGQENIITAGVKGERTHYIS
                     VLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESGLAPTTEVKPRLDIQKEEI
                     PFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADGKEVKTLVNSVVAQEAVTQ
                     IVEVGTMVTHVGDENGQAAIAEEKPKLEIPRQPAPSTAPAEESKALPQDPAPVVTEKK
                     LPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(22988..23704)
                     /locus_tag="VW049_000714"
     CDS             complement(22988..23704)
                     /locus_tag="VW049_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9431876.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT         6440 a         4730 c         5687 g         6914 t
ORIGIN      
        1 tattttctag gtagaacgtt tgttctgcct atttttttat ccaaaaagtg cagttgggag
       61 ggagataggc tcatttggga aggaagtcca gtttttgttt agtgattggg gtaagatagt
      121 tgttatcaga tgagtttata ctcttcgaaa atcaaattca aaccacgtca acgtcgcctt
      181 gccgtatata tgtgactgac ttcgtcagtc ctatctacaa cctcaaaaca gtgttttgag
      241 cagcctgcgg ctagtttcct agtttgctct ttgattttca ttgagtatta gggaaaagga
      301 gatgaatatg aaatttggga aacgtcacta tcgtccgcaa gtggatcaga tggactgcgg
      361 tgtagcttca ttagccatgg tttttggcta ctatggtagt tattattttt tggctcactt
      421 gcgagaattg gctaagacga ccatggatgg gacgacggct ttgggcttgg tcaaggtggc
      481 agaggagatt ggttttgaga cgcgagccat taaggcggat atgacgcttt ttgacttgcc
      541 ggatttgact tttccttttg ttgcccatgt gcttaaggaa gggaaattgc tccactacta
      601 tgtggtgact gggcaggata aggatagcat tcatattgcc gatccagatc ccggggtgaa
      661 gttgactaaa ctgccacgtg agcgttttga ggaagaatgg acaggagtga ctctttttat
      721 ggcacctagt ccagactata agcctcataa ggaacaaaaa aatggtctgc tctcttttat
      781 ccctatatta gtgaagcagc gtggcttgat tgccaatatc gttttggcaa cactcttggt
      841 aaccgtgatt aacattgtgg gttcttatta tctgcagtct atcattgata cctatgtgcc
      901 agatcagatg cgttcgacac tagggattat ttctattggg ctagtcatcg tctacatcct
      961 ccagcaaatc ttgtcttacg ctcaggagta tctcttgctt gttttggggc aacgcttgtc
     1021 gattgacgtg attttgtcct atatcaagca tgtttttcac ctccctatgt ccttctttgc
     1081 gacacgcagg acaggggaga tcgtgtctcg ttttacagat gctaacagta tcatcgatgc
     1141 gctggcttcg accatccttt cgattttcct agatgtgtca acggttgtca ttatttccct
     1201 tgttctattt tcacaaaata ccaatctctt tttcatgact ttattggcgc ttcctatcta
     1261 cacagtgatt atctttgcct ttatgaagcc gtttgaaaag atgaatcggg ataccatgga
     1321 agccaatgcg gttctgtctt cttctatcat tgaggacatc aacggtattg agactatcaa
     1381 gtccttgacc agtgaaagtc agcgttacca aaaaattgac aaggaatttg tggattatct
     1441 gaagaaatcc tttacctata gtcgagcaga gagtcagcaa aaggctctga aaaaggttgc
     1501 ccatctcttg cttaatgtcg gcattctctg gatgggggct gttctggtca tggatggcaa
     1561 gatgagtttg gggcagttga ttacctataa taccttgctg gtttacttta ccaatccttt
     1621 ggaaaatatc atcaatctgc aaaccaagct tcagacagcg caggttgcca ataaccgtct
     1681 aaatgaagtg tatctagtag cttctgagtt tgaggagaag aaaacagttg aggatttgag
     1741 cttgatgaag ggagatatga ccttcaagca ggttcattac aagtatggct atggtcgaga
     1801 cgtcttgtcg gatatcaatt taaccgttcc ccaagggtct aaggtggctt ttgtggggat
     1861 ttcagggtca ggtaagacga ctttggccaa gatgatggtt aatttttacg acccaagtca
     1921 aggggagatt agtctgggtg gtgtcaatct caatcagatt gataaaaaag ccctgcgcca
     1981 gtacatcaac tatctgcctc aacagcccta tgtctttaac ggaacgattt tggagaatct
     2041 tcttttggga gccaaggagg ggacgacaca ggaagatatc ttacgggcgg tcgaattggc
     2101 agagattcga gaggatatcg agcgcatgcc actgaattat cagacagaat tgacttcgga
     2161 tggggcaggg atttcaggtg gtcaacgtca gagaatcgct ttggcgcgtg ctctcttgac
     2221 agatgcgccg gtcttgattt tggatgaggc gactagcagt ttggatattt tgacagagaa
     2281 gcggattgtc gataatctca ttgctttgga caagaccttg attttcattg ctcaccgctt
     2341 gactattgct gagcggacag agaaggtggt tgtcttggat cagggcaaga ttgtcgaaga
     2401 aggaaagcat gctgatttgc ttgcacaggg aggcttttac gcccatttgg tcaatagcta
     2461 gaaagaggag aggatgaaac cagaattttt agaaagtgcg gagttttata atcgtcgtta
     2521 ccataatttt tccagtagtg tgattgtacc catggccctt ctgctcgtgt ttttacttgg
     2581 ctttgcaact gttgcagaga aggagatgag tttgtccact agagctactg tcgaacccag
     2641 tcgtatcctt gcaaatatcc agtcaactag caacaatcgt attcttgtca atcatttgga
     2701 agaaaataag ctggttaaga agggggatct tttggttcaa taccaagaag gggcagaggg
     2761 tgtccaagcg gagtcctatg ccagtcagtt ggacatgcta aaggatcaaa aaaagcaatt
     2821 ggagtatctg caaaagagcc tgcaagaagg ggagaaccac tttccagagg aggataagtt
     2881 tggctaccaa gccacctttc gcgactacat cagtcaagca ggcagtctta gggctagtac
     2941 atcgcaacaa aatgagacca tcgcgtccca gaatgcagca gctagccaaa cccaagccga
     3001 aatcggcaac ctcatcagtc aaacagaggc taaaattcgc gattaccaga cagctaagtc
     3061 agctattgaa acaggtactt ccttggccgg tcagaatcta gcctactctc tttaccagtc
     3121 ctacaagtct cagggcgagg aaaatcccca aactaaggtt caggcagttg cacaggttga
     3181 agcacagatt tctcagttag aatctagtct tgctacttac cgtgtccagt atgcaggttc
     3241 aggtacccag caagcctatg cgtcagggtt aagcagtcaa ttggaatccc ttaaatccca
     3301 acacttggca aaggttggtc aggaattgac ccttctagcc cagaaaatct tggaggcaga
     3361 gtcaggtaag aaggtacagg gaaatctttt agacaagggg aaaattacgg caagtgagga
     3421 tggggtgctt catcttaatc ctgagaccag tgattctagc atggttgcag aaggtgccct
     3481 actagcccaa ctttatccat ctttggaaag agaagggaaa gccaaactca cagcctatct
     3541 aagttcaaaa gatgtagcaa gaatcaaggt cggtgattct gttcgctata ctacgactca
     3601 tgatgccggg aatcaacttt ttctagattc tactattaca agtattgatg cgacagctac
     3661 taagactgag aaagggaatt tctttaaaat cgaggcggag actaatctaa cttcggagca
     3721 ggctgaaaaa cttaggtacg gggtggaagg ccgtctacag atgattacgg gcaagaaaag
     3781 ttatctacgt tattatttgg atcaattttt gaacaaagag taatgttcgt gtttttagag
     3841 ttaaataatt tttaaactgt gagaaagatt cttcttgcag ttttttcttt acaatttttg
     3901 aaaaacatct gctatttatt cggttaaatt cttgtgtttt ttggtttttt gtggtaaaat
     3961 gtgctcaagt aatacgaaag gcgaacttta aaatgtcaaa acaattgatc tattcgggaa
     4021 aagctaaaga tatctataca actgaggatg aaaatcttat tatttcaact tacaaggacc
     4081 aggcgactgc tttcaatggt gtcaagaagg agcagattgc aggtaaggga gtcttgaata
     4141 atcagatttc atcttttatt tttgagaaat taaatgcggc tggtgtggcg actcactttg
     4201 tggagaaact ttcagacacg gaacaactca ataaaaaggt taagattatt cctttggaag
     4261 tcgtgctccg caactatact gctggttcct tttcaaaacg ttttggtgtg gatgagggaa
     4321 tcgccttgga gactccgatt gtcgaatttt actacaaaaa tgatgatttg gatgatccat
     4381 ttatcaatga tgagcatgtg aaattcctac agattgcgga tgaccagcag attgcctact
     4441 tgaaggaaga aacgcgtcgt atcaatgaac tattgaaagt ctggtttgct gagattgggc
     4501 ttaaattgat tgactttaag ctagagttcg gttttgacaa ggatggcaag attatcttgg
     4561 cagacgaatt ttcaccagat aactgccgct tgtgggatgc tgatggcaac cacatggata
     4621 aggatgtttt ccgtagagga ttgggagaac taaccgacgt ttatgagatt gtttgggaaa
     4681 agttgcaggg tttaaaataa caacctcaag gctgtttggg aatattgcaa gagctgaaat
     4741 aaaggaataa gaattgatgg ataaacgtat ttttgttgaa aaaaaggctg attttcaggt
     4801 caagtcagag agtttggtta gagagctcca gcacaacttg ggactgtcaa gcttgaaaag
     4861 tattcgtatt gtgcaagtat atgatgtatt tgacttggtt gaggacttgt ttgcacctgc
     4921 agagaagcac attttctctg agcaggtaac cgaccatgtt ttagatgaag tatctgtgca
     4981 ggcggatctt gctaactatg ctttctttgc cattgaaagt ctaccagggc agtttgacca
     5041 gcgtgcagct tcgtcacagg aagccttgct tttgttggga agttcgagtg acgtgacagt
     5101 caacacagcc caactttact tggtgaataa agatattgat gcgactgagt tggaagctgt
     5161 caaaaactac ctgctcaatc cagttgattc tcgtttcaag gatatcacga tagggattgc
     5221 caagcaggag ttttcagagt cagacaagac cattccaaaa ttgcctttct ttgaaagcta
     5281 tacagcagaa gactttgctc gctacaaggc cgaacaaggg atggccatgg aagtggatga
     5341 tttgctcttt atccaagact actttaagtc aatcgggcgc gtgccaactg agactgaact
     5401 caaggttttg gacacttact ggtctgacca ctgccgtcat acgacttttg agacagagtt
     5461 gaaacacatc gacttttcag cttctaaatt tcaaaagcaa ttgcagtcaa cctatgacaa
     5521 gtatattgcc atgcgcgagg aattaggtcg gtctgaaaaa ccacaaacct tgatggatat
     5581 ggcgactatt ttcggtcgtt atgagcgtgc taatggacga ttggatgata tggaagtctc
     5641 tgacgaaatc aatgcctgct cagttgaaat tgaagtggac gttgatggtg tcaaggaacc
     5701 ttggctcctc atgtttaaaa acgaaactca caaccatcca acagaaattg agccatttgg
     5761 tggagcggct acctgtatcg gtggagctat tcgtgatccg ttgtcaggcc gttcctatgt
     5821 ttaccaagcc atgcgtattt caggtgctgg tgatattaca gcaccgattt cggaaactcg
     5881 tgctgggaaa ttgccacaac aagtcatttc taaaacagca actcatggtt attcttcata
     5941 tggtaaccag attgggcttg ccacaaccta cgttcgtgaa tactttcacc caggctttgt
     6001 agctaaacgt atggaacttg gtgccgttgt tggtgcggct cccaagggca atgttgtccg
     6061 tgaaaaacct gaagcaggtg atgtgatcat ccttctcgga ggtaaaacag gtcgtgatgg
     6121 tgtcggtggt gcgacgggct cttctaaggt tcaaacagtt gagtctgtag agactgctgg
     6181 tgctgaggtt caaaaaggaa atgccatcga agaacgcaag attcagcgcc tcttccgtaa
     6241 tggcaatgtc actcgtctga tcaagaagtc caatgacttt ggggcaggcg gtgtctgtgt
     6301 ggctatcggt gaattggcag acggtcttga aatcgacctc aacaaggtgc ctcttaaata
     6361 ccagggcttg aatggtacag aaattgccat ctctgaatca caagaacgga tggcggtcgt
     6421 ggttcgtcct gaagatgtgg ataccttcgt tgccgaatgt aacaaagaaa atattgatgc
     6481 tgttgtggtg gcgacagtaa ctgaaaaacc aaatcttgtc atgcactgga atggtgagac
     6541 aatcgttgac ttggagcgtc gtttccttga catcaatggt gtgcgcgtgg ttgtcgatgc
     6601 caaagttgtg gacaaggatg tcaaactccc agaagagcgt caaacatctg ctgaaacact
     6661 ggaatcagat acccttacgg ttctatctga cctcaaccat gcaagtcaaa aaggattaca
     6721 gactatcttt gactgctctg ttggacgctc aacggttaat cacccacttg gtggtcgtta
     6781 ccaactcaca ccaactgagg catctgtgca gaaattgcca gttcaacacg gtgtgactca
     6841 tactgcgtcg gtcattgctc aaggtttcaa cccatatgta gctgaatggt ctccatacca
     6901 cggtgctgct tatgcggtta tcgaagcaac tgctcgtttg gtggctgttg gtgccaactg
     6961 gtccaaggct cgtttctctt accaagaata tttcgagcgt atggacaagc aagcagagcg
     7021 ttttggtcag ccagtagccg ctcttctagg ctctattgaa gctcagattc aacttggctt
     7081 gccatctatc ggtggtaagg actccatgtc tggtaccttt gaagaattga ccgttccgcc
     7141 aaccttggtt gcctttgggg tgacgacggc agatagccgt aaggtgctct ctccagaatt
     7201 taaagctgtt ggggaaaata tctactacat cccaggtcaa gccctctctg cagagattga
     7261 ttttgacttg attaagaaaa attttgctca gtttgaagca agccaagctg accataaagt
     7321 gacatctgca tcagctgtca aatacggtgg tgtagttgaa agtttggctc ttgctacctt
     7381 tggaaactat attggtgcag aggtgacctt gcctgaactt aaaacagctt tgacagctca
     7441 attaggcggc tttgtcttca catctcctga agaaattgct ggagtagaga agattggaca
     7501 aacgaaagca gactttacac tgactgtcaa cggtgtgaag ctagatggac acaagcttga
     7561 cagtgcattt caagggacat tggaagaagt ttacccaaca gaatttaccc aagcgaaaga
     7621 actagaagaa gtaccagctg tggcatcaga tgttgtgatt aaagccaaag aaaaggttga
     7681 aaaacctgtg gtttacatcc cagtctttcc aggaaccaac tcagaatatg attcagctaa
     7741 ggccttcgaa aaagaaggtg cagaggtcaa tttggtgcca ttcgtgacct tgaatgaaga
     7801 agctattgtc aagtcagttg aaactatggt tgacaatatc gacaagacta atattctctt
     7861 ctttgctggt ggattctcgg ctgcggatga accagatggt tcagctaagt ttatcgtcaa
     7921 tatcctgctt aatgaaaaag tgcgtgtggc tattgatagc tttatcgccc gtggtggttt
     7981 gattatcggt atttgtaatg gattccaagc cttagtcaaa tcgggtctcc taccctacgg
     8041 aaactttgaa gatgctaaca atactagccc aaccctcttc tacaatgatg ccaaccaaca
     8101 cgtggccaag atggtggaaa ctcgcattgc caataccaac tcaccatggt tggctggtgt
     8161 gcaagtgggc gatatccacg ctattcctgt ttcgcacggt gaagggaagt ttgtcgtgac
     8221 ggctgaggaa tttgcagagc tccgtgacaa tggacaaatt ttcagccaat acgttgactt
     8281 taacggtaaa ccaagtatgg attctaagta caatccgaat ggttctgtcc atgccatcga
     8341 aggaattacc agcaagaatg gtcaaatcat cggtaagatg ggccattcag aacgttatga
     8401 ggacggtctt ttccaaaata tcccaggcaa taaagaccag cacctgttcg cgtcagcggt
     8461 taaacatttc actggaaaat aagacttaca gattttctaa tagatagtat cagtaatgta
     8521 aaagtcatgt aaatctagct cttgatgatt acaaatgaaa attaggtata aaaaatgaca
     8581 tacgaagtaa aatctcttaa tgaagaatgt ggtgttttcg gtatttgggg acatccagat
     8641 gctgctaagt tgacctattt tggactccac agtcttcaac accgtggtca ggagggggca
     8701 ggaatcctct ccaatgatca aggacaactg aagcggcatc gcgatatggg tcttctatca
     8761 gaagtcttca gaaatcctgc taacttagat aaattgacgg gaactggtgc gattgggcat
     8821 gtgcgttacg cgactgctgg cgaagcttct gtagataata tccagccctt cctcttccgt
     8881 tttcacgata tgcagtttgg cttggctcat aatggaaatc tgaccaatgc agcctctctc
     8941 aagaaagaac tggaacaaag aggagcaatt ttcagcgcga cttcggactc tgaaatcttg
     9001 gctcacctca ttcgtcgcag tcataatcct agcctgatgg gcaaaatcaa ggaagcgctc
     9061 agccttgtca aaggtggttt tgcctatatc ttgctgtttg aggacaagtt gattgcggct
     9121 cttgacccaa atggattccg accgctttcg attggtaaaa tggctaatgg agcagttgtt
     9181 gtatcttctg aaacctgtgc ttttgaggtc attggtgccg agtggattcg tgatttgaag
     9241 ccaggtgaga ttgtgatcat tgatgacgag ggcattcagt atgacagcta tacagatgat
     9301 acccagttgg cggtttgttc tatggagtat atctactttg ctcgccctga ttctaatatc
     9361 cacggtgtca atgtccatac ggcacgtaag agaatgggag cgcaattggc gcgagaattt
     9421 aagcatgagg cagatattgt agttggtgtg cccaattctt ccctaagcgc ggctatggga
     9481 tttgcggaag aatcaggctt accaaatgaa atgggtctga tcaaaaacca atacacccag
     9541 cgaactttta tccaaccgac tcaagaattg cgggagcaag gagtgcggat gaaactgtct
     9601 gctgtttcgg gtgttgtcaa aggcaaacgt gtggtcatgg tggatgattc cattgtacgt
     9661 ggaacaacct ctcgtcgtat cgttcagctc ttgaaagaag cgggtgcgac tgaggttcac
     9721 gttgccattg gaagtcctgc actagcgtat ccatgtttct acgggattga tatccagacc
     9781 cgtcaggagc tgattgcagc caatcatacg gtcgaagaaa ctcgccaaat cattggtgcg
     9841 gacagtatga cttatctttc aattgatggc ttgattgagt cgattggtat cgaaacagat
     9901 gcgccgaacg gtggtctctg tgtcgcttac tttgacggtg actacccaac gcctctctat
     9961 gactacgaag aagactatcg tagaagtttg gaagaaaaga ccagttttta caagtaggcg
    10021 acagattctc cattaaagaa aaggaaaaaa caaatgacaa ataaaaatgc atatgcccag
    10081 tcgggtgtgg atgttgaagc gggttatgaa gttgttgaac ggattaaaaa gcacgtggcc
    10141 cgtacggagc gtgcaggtgt catgggagct cttggtggct ttggtggtat gtttgacctt
    10201 tccaagactg gggttaaaga acccgtcttg atttcaggga ctgacggtgt cggaaccaag
    10261 ctcatgttgg ctatcaagta cgacaagcac gataccatcg ggcaggactg tgtggccatg
    10321 tgtgtcaacg acatcattgc tgcaggtgcg gaacccctct attttctcga ctacgtagcg
    10381 acagggaaga atgaaccagc taagctagaa caagtggttg ctggtgtggc agaaggttgt
    10441 gtgcaggctg gtgctgccct catcggtggg gaaacggctg aaatgccggg catgtacggc
    10501 gaagacgact atgacttggc tggttttgcg gtcggtgtgg ctgaaaaatc tcaaatcatt
    10561 gacggttcaa aggtggtaga gggagatgtt cttctcggac ttgtttcaag tgggattcac
    10621 tcaaatggtt actctttggt tcgtcgtgtc tttgcggatt acacaggtga ggaagtccta
    10681 ccagaattgg aaggcaagaa acttaaggaa gttctacttg agccgactcg tatctatgtc
    10741 aaggctgtct tgccgctcat caaagaagag ttggtcaacg gcattgccca catcacaggt
    10801 ggtggcttta tcgaaaatgt ccctcgtatg tttgcagatg acctagctgc tgaaattgat
    10861 gaaagcaaag ttccagtgct tccaattttc aaagcccttg aaaaatatgg tcagattaaa
    10921 cacgaagaaa tgtttgaaat cttcaatatg ggtgtgggac ttatgttggc ggtcagccct
    10981 gaaaatgtag agcgtgtaaa agaattgttg gatgaagcag tctatgaaat tggtcgcatc
    11041 gtcaagaaag aaaacgaaag tgtcattatc aaatgaaaaa aatagcggtt tttgcctctg
    11101 gtaatggctc aaattttcag gtgattgccg aagaatttcc agtggagttt gtcttttcag
    11161 accatcgtga tgcctatgtg cttgagcgtg caaagcagct cggcgttctg tcctatgctt
    11221 ttgaactcaa ggagtttgag agcaagacag actacgaagc agcccttgtc gaactcttgg
    11281 aagaacacca gattgacttg gtttgcctag caggctacat gaaaatcgtt ggaccaacct
    11341 tattgtcggc ttatgaaggt cggattgtca acattcatcc agcctatttg ccagaatttc
    11401 caggagctca tgggattgag gatgcttgga atgctggcgt gggtcagtct ggtgtgacca
    11461 ttcactgggt ggattcgggt gtggatacag gccaggtcat caaacaggtt cgtgtgccac
    11521 gactagctga tgataccatt gacagatttg aagctcgcat ccatgaagca gagtacaggt
    11581 tgtatccgga agtagtgaag gctctattta cagattgact ttttgatgat tcatatgata
    11641 tctttgattt taaattggag tcagtgtttg ttgaagacgg cttcaaacgg aggtatttgt
    11701 aatgttagaa tctaaaaaaa caactcgata tgtattttat gtctatctga tgttattaac
    11761 ttggggaatc ttatttaagt ttgaaacaaa tcctgaattt atagcatttt tcttagctcc
    11821 aaggtatatc aattggattc cattttcaga accactaata gtcgatggaa aaattgtttt
    11881 tgctgaaatg ttatttaatc tgatttcctt tattccatta ggtgtttgtt tccctttgat
    11941 aaaaactaat ttatctagtt taagaatagt cgggacaggt ttcttgatta gtttattgtt
    12001 tgagtgctta cagtatattt tagcaatagg tataacagat ataacggatt tgactttaaa
    12061 tacgctaggt gtctgtgtag gcttactgat ttatcaaatt tttataagag tgttcaaatc
    12121 acagactaga aaatggatca atatcttagg tatgcttagc cttggttttg cttatcttgt
    12181 tttactgtta ctgcatttaa ttggtgttta actaatgatt aaaaaggaga atataatgac
    12241 taaacgcgtc ttaatcagcg tctcagacaa agcgggcatt gttgaatttg cccaagaact
    12301 aaaaaaactt ggttgggaga ttatctcgac aggtggaact aaggttgccc ttgataatgc
    12361 tggggtggag accattgcga tcgatgatgt gacgggcttc ccagagatga tggatggccg
    12421 tgttaagact cttcatccaa atatccacgg gggtcttctc gctcgtcgtg atttggatag
    12481 ccacttggaa gcagccaatg agaaccaaat tgagcttatt gaccttgtag tggtcaacct
    12541 ttatcctttc aaggaaacga ttctcaaacc agacgtgacg tacgctgatg ctgtggagaa
    12601 tattgatatt ggtgggccat ctatgcttcg ttcagcagcg aagaatcatg ccagcgtgac
    12661 ggttgtggta gaccctgctg actatacagt ggttttggac gagttgtcag ccaatggtga
    12721 aacgacttac gaaacgcgtc aacgtttggc agccaaggtt ttccgtcaca cagcggctta
    12781 tgacgctttg attgcagaat acttcacagc tcaagtgggc gaaagcaaac ctgaaaagtt
    12841 gaccttgacc tatgacctaa aacaagctat gcgttatggg gaaaatcccc agcaggatgc
    12901 ggacttctac cagaaagctt tgccgactga ttactccatt gcatcagcca aacagcttaa
    12961 cggtaaggaa ttgtcattca acaatatccg cgatgcagat gctgccattc gtattatccg
    13021 tgacttcaaa gaccgtccaa ccgttgtggc tctcaagcat atgaatccat gtggtatcgg
    13081 ccaggcggat gacatcgaga cggcttggga ttatgcttat gagtctgacc cagtgtctat
    13141 ctttggtggt atcgctgttc ttaaccgtga ggtggatgct gcgacagctg agaagatgca
    13201 cggcgttttc ctcgaaatca tcattgcacc aagctatacg gatgaagcgc tagccatttt
    13261 gatcaataaa aagaaaaact tgcgtatcct tgccttgcca tttaatgctc aagaggctag
    13321 cgaagtggaa gcagaataca caggtgtagt cggtggactt ctcgtgcaaa atcaagacgt
    13381 ggtcaaggaa agcccagctg actggcaagt ggtgaccaaa cgtcagccaa ccgagacaga
    13441 agcgactgct cttgagttcg cttggaaggc tatcaagtac gtcaaatcaa atggtattat
    13501 cgtgaccaac gaccacatga cacttggtgt tggtccaggt caaaccaacc gtgtggcttc
    13561 tgttcgcctt gccattgacc aagccaaaga tcgtctggac ggggcggtcc ttgcttcaga
    13621 tgccttcttc ccatttgcgg ataacgtgga agaaatcgcc aaagcaggaa ttaaggccat
    13681 catccagccc ggtggctctg tccgtgacca agaatccatc gaagcagcgg ataaatacgg
    13741 cttgactatg gtctttacag gtgtgagaca ttttagacat taagaagata aaagggaaga
    13801 aaacagtttc tttccttttt tggcttaaaa tgctaactga aacaagatta aaacgaactt
    13861 ttttgatata atgttggtaa ataattcgca aaagaggttg aggaatgaaa ctacttgttg
    13921 tcggttctgg tggtcgtgag catgcgattg ctaaaaagtt acttgaatca aaagacgtgg
    13981 aaaaagtctt tgtagctcct gggaatgatg ggatgactct ggatggtttg gaattggtaa
    14041 atatctctat ttccgaacat tctaaattga ttgacttcgc aaagaccaat gatgttgctt
    14101 ggacctttat cggtccagat gatgcccttg ctgctggtat cgtggatgat tttaaccaag
    14161 ctggacttaa ggcctttggt ccgactaggg ctgcagcgga gctggagtgg tccaaggatt
    14221 tcgccaagga aatcatggtc aaatacggcg ttccgacagc aacatatggc acattttcag
    14281 atttcgagga agccaaggcc tatatcgaaa atcatggtgc gcctatcgta gtcaaggcgg
    14341 atggcttggc acttgggaag ggtgtcgtcg ttgctgagac ggttgagcaa gcggtcgaag
    14401 ccgctcatga gatgcttttg gacaataaat ttggtgactc aggtgcgcgc gtggttattg
    14461 aggaattcct tgaaggagag gaattttcac tctttgcctt tgtaaatggt gataagttct
    14521 acatcatgcc aatggctcag gaccacaaac gtgcctatga tggcgacaaa gggcctaaca
    14581 cgggtggtat gggtgcctat gcgccagtcc cacacttacc acagagtgta gttgatacag
    14641 cggttgacac cattgtcaag ccagttctag aaggggtgat taaagaaggt cgcccttatc
    14701 tgggagttct ttacgcaggg cttatcctga cagctgatgg accgaaagtc attgagttca
    14761 acgctcggtt cggagatcca gaaactcaga ttatcttgcc tcgcttgacc tctgactttg
    14821 ctcaaaatat cacagatatc ctggatagca aggagccaaa tatcatgtgg acggacaagg
    14881 gtgtgactct gggtgtggtt gtcgcatcca agggctaccc gctagactat gaaaggggcg
    14941 ttgagttgcc agcaaagaca gaaggcgatg tcatcaccta ctatgcaggg gctaagtttg
    15001 cggaaaatag cagagcactg ctctcaaacg gcggacgagt ttatatgctc gttaccacag
    15061 cagataccgt caaagaagcc caagccagca tctaccaaga actatcccaa caaaaaataa
    15121 aaggactctt ctaccgaaca gatatcggaa gcaaggcaat taagtaaaga tataagaata
    15181 acgcgccgta gtcgccaaac acgataatgg tcgtcgtggt gaaaagacca gaacagtgaa
    15241 tgttctggtc agggggaaac ttggagacct taggctcaaa gtttaggaat gaaaccgaag
    15301 gtttgcttcc gcctccatca cctaagacca ttatcaaaaa gaaaaataaa aattcacaaa
    15361 atacgttaat gatcgtatgg tttgcgagcg ttagcgagct aatatagaac aatcaccgcc
    15421 gttgtgaaag aacgattgga tgataatcca atcgttcagg gaaattggaa gaccttgggt
    15481 ttccaattta ggcatgagac acctttggtg gctgctgccg tccctcacaa gctaaggtga
    15541 ttgttgaaaa agaggaaaaa ggagaagaaa tgaaaccagt aatttccatc atcatgggct
    15601 caaaatccga ctgggcaacc atgcaaaaaa cagcagaagt cctagaccgc ttcggtgtag
    15661 cctacgaaaa gaaagttatt tccgcacacc gtacaccaga cctcatgttc aaacatgcag
    15721 aagaagcccg tagtcgtggc atcaagatca tcatcgcagg tgctggtggc gcagcgcatt
    15781 tgccaggcat ggtagctgcc aaaacaaccc ttccagtcat tggtgtgcca gtcaagtctc
    15841 gtgctcttag tggagtggat tcactctatt ctatcgttca gatgccgggt ggggtgcctg
    15901 ttgcgaccat ggctatcggt gaagctggag cgactaacgc agctctcttt gccctccgtc
    15961 tcctctctgt agaagatagg tccattgcgg atgcacttgc caactttgct gaagaacaag
    16021 gaaaaatcgc agaggagtcg tcaaatgagc tcatctaaaa caatcggaat tatcggtggc
    16081 ggtcaactgg gtcagatgat ggccatttct gctatctaca tgggccacaa ggttatcgcg
    16141 ctggatcctg cggcggattg cccggcctct cgcgtggcgg aaatcattgt ggcaccttat
    16201 aacgatgtgg acgccctccg tcagttggca gaccgttgcg atgtcctcac ttacgagttt
    16261 gaaaatgtcg acgctgacgg tttggatgcc gttatcaagg atggacaact ccctcaagga
    16321 acagatctgc tccgcatttc gcaaaatcgt atttttgaaa aggacttttt gtcaaacaag
    16381 gctcaagtca ctgtggcacc ctacaaggtc gtgacttcta gcctagactt ggcagatatc
    16441 gacttgtcga aaaactatgt cctcaagact gcgactggtg gctacgatgg tcatggacaa
    16501 aaggttattc gttcagaagc agacttggaa gcagcctatg cgctagcaga ctcagcagac
    16561 tgcgtcttgg aagaatttgt caactttgac cttgagattt ctgtcatcgt gtcaggaaat
    16621 ggcaaggagg tgacgttttt cccagttcag gaaaatatcc accgcaacaa tatcctgtct
    16681 aagaccatcg taccagcccg catttctgaa agtctagcag acaaggctaa agctatggca
    16741 gtgcgaatcg cagaacaact caacttgtct ggaactctct gtgtggaaat gtttgcgact
    16801 gatgatgacg ttattgtcaa tgaaatcgcc ccacgaccac ataactctgg gcactattct
    16861 attgaagcct gtgatttctc tcagtttgac acccatattc tgggtgttct cggagcacca
    16921 ttaccagtca tcaaactcca tgcgccagcc gttatgctta atgtcctcgg tcagcatgtc
    16981 gaggctgctg agcaatatgt gacagaaaat tcaagcgccc acctccacat gtatggtaaa
    17041 atagaagcga agcacaaccg caagatggga catgtgacct tgtttagtga tgtaccggat
    17101 agtgtggatg agcttgggga agggattgat ttttaggaca agtctatgat acaaattatc
    17161 gttaatacat ttattgaaaa ggataagact ggagcagttg ttgaagtgtt gtatgccagt
    17221 gctgaccaag ataaggtaca agctaaatat gaagaactag ctgcacaata ccccgaaaat
    17281 tatttagcta tctatgatgt accgctggat acggatttga atacactaga tcattacccg
    17341 tctgtgttta ttgaaaaaga ggagtttgag tagaaatctt ggtttaccta gatagcttat
    17401 tcccaacagc ttaagaagaa aggaaaaatt aacacatgat caaccgttac tctcgccctg
    17461 agatggcgaa tatttggagt gaagaaaata aataccgtgc ttggcttgag gtggaaatcc
    17521 tctctgacga ggcatgggct gagttggggg aaatccctaa ggaagatgtg gctttgattc
    17581 gcaagaaggc ggactttgac atcgaccgta ttttggaaat tgagcaggag acgcgccacg
    17641 atgtggtggc tttcacgcgt gcggtttctg agactcttgg tgaagagcgc aagtgggttc
    17701 actatgggtt aacttctacc gacgtggtgg atactgctta tggttacctc tacaagcagg
    17761 ccaacgacat catccgtcgt gaccttgaaa acttcactaa tatcatcgct gacaaggcca
    17821 aggagcacaa gttcaccatc atgatggggc gtactcatgg tgtgcacgct gagccgacaa
    17881 cctttggtct taaattggca acttggtaca gcgaaatgaa acgcaatatc gagcgtttcg
    17941 agcatgcagc tgctggtgta gaagctggta agatttctgg tgcggttggg aactttgcca
    18001 atatcccacc atttgtagag gagtatgtct gcgataaact tggcatccgt gcccaagaaa
    18061 tctctacaca agtccttcct cgtgaccttc acgctgagta ctttgcggtt cttgccagca
    18121 ttgcgacttc aatcgaacgt atggcgactg agattcgtgg tctacaaaaa tctgagcaac
    18181 gcgaagtaga agagttcttt gctaaagggc aaaaagggtc ttcagcaatg cctcacaaac
    18241 gcaacccaat cggttctgaa aatatgactg gtctggcgcg tgtcattcgt ggtcacatga
    18301 ttacggctta tgaaaacgtc gctctctggc acgaacgcga tatttctcac tcatcagctg
    18361 agcgtatcat cacaccagat acgaccattt tgattgacta catgctcaac cgttttggaa
    18421 atatcgtcaa gaacttgaca gtcttcccag aaaatatgat ccgaaacatg aactcgactt
    18481 ttggtcttat ctttagccaa cgggctatgt tgacattgat tgaaaaaggc atgacccgtg
    18541 agcaagccta tgacttggtg caaccaaaaa cagcctactc ttgggacaac caagtagact
    18601 ttaaaccact tcttgaggca gattcagaag taacatcacg tctcactcaa gaagaaatcg
    18661 atgaaatctt caacccagtt tattacacca aacgagtgga tgatatcttt gaacgtcttg
    18721 gactaggtga ttaattaaaa aataaacagc gagcttcaat ctcgctgttt attttttatc
    18781 gaaaagactt agtcttcttt tcttttagtg agtccatagg ctgctagtgt ggacatgagt
    18841 cctgcgacta ctagtcctgc agaatcgtga gttcctgttt caggaagttt tttctctgtt
    18901 accacaggag ctggatcttg aggaagagct ttgctttcct cagcaggagc agttgatgga
    18961 gctggttgtc ttgggatttc tagttttggt ttttcttcag caatagcggc ttgtccgttt
    19021 tcatcgccta catgtgttac catagttccg acttcgacta tttgagtaac ggcttcctgt
    19081 gctacgacac tatttacaag tgttttcact tccttaccat cggcagaagt gctcacagag
    19141 tagaagttgc tacgatgtcc attgacgccc ttagtaatga cttgtgtttt tcctttgagt
    19201 aagagtggat tttcacgagt cactgtggta aatggaattt cttctttttg gatatccagt
    19261 ctaggtttta cctcagtagt tggtgcaaga ccactttcat cacccttgtg agttacagga
    19321 gcgccaactt caaccacttg gtttataact tctttggtta cctggctatc aaggactgtt
    19381 tctgttgttt ttccattttc agtgagtaca gagatgtaat gagttcgttc acctttgact
    19441 cctgctgtga taatattttc ctgaccagct gggaggttag gattttcttt cttgataact
    19501 tcaaatggaa tttcttcagt tcttgtgatg agttctggtc tggtttcaac attggcagcc
    19561 acttcatttt catctaggct tcctgaatga gttgcagctg gtttgaggcc ttgaagagcg
    19621 gcttttaggt tggctacaag tgtgtcaagc tcagcttgtt tattacggtt gaggttgtaa
    19681 tttagagctg ttttagctgc gtcaagggcc tcaagacttt ctttactata tccttctaag
    19741 tttgtaggaa ttttagctaa ttcttcgcgg agagcattat aattagcacg gaagtagtct
    19801 ttgttgtggt ctgcaaaggc agtcatgagt tcaaagattt cctcttcctt gtattcagcg
    19861 cttggtctat ctgcccagat tgaaagcata cttccgactg ttggaagatc tacttcagga
    19921 tatttggtag aagctagttg attgaatggt gtttttccag tattctcaat agctttcttg
    19981 aggaaaccac caccatcttc tggtttttga ccaagaatgt agtaccagtc accgttggta
    20041 ttcaagaatt tatagccttt gcttgctagg tattgaggtg atgcgaggtt atatccccac
    20101 cagcctttag accagtaaga aatcaagaca tctttgtcaa actgaacatc gtccttgtct
    20161 tcatagtaga agccatcgtt gaaggccatt ggttgaagcc ctctttcttt ggccatagct
    20221 gcgagggtgt tggcatattc ggcaaatttg ccatagagtt gataccactt gaggtagtac
    20281 cagccttggg cactagtcgc atcgttggcg tattcgtcag taccaaagtt gaaaatcttt
    20341 gttttacctg caaagaagtc catgtattta ccgatgaggg cttttacaaa gttcatcgct
    20401 tcttcgtttt tcaagtccat agttgttttt gaaactttat caaagtgggc ttgaggattt
    20461 ttaataccta atttttccat ggcaaccagc atagcatcca tgtgacctgg actgttaata
    20521 gctgggatga gaccaatgtc cttagattta gcgtattcaa ttagctctgt cacttctgcc
    20581 tgtgttagtg cagtaccgtt tggatcgtcg tagtaagctt tagttccttc gataatagct
    20641 tttttaacgt catcactagc ataggttttt ccgttggcag taatggtcat atcattgagt
    20701 agaaagcgaa gtccgtcatt tcctagaagg agatggacat cagaatatcc gagctcactg
    20761 gccttgtcta cgatgcgttt gagctggtcc agagtaaagt atttgcgtcc agcatcgatt
    20821 gagattacct tgtttttggc aagtttttca acctcacgtt tagcttcttc ttctttttga
    20881 gcttcaggcg tgagggtcaa gttgttgaca gtttcttgaa gtttagcaat ggcttgatca
    20941 atcgtatctt gttgggcacg gctaaggttg ctatcgagag agcgaatagc tttttcagct
    21001 tcttttacgg ccgcgacgct ttctgcagta taacggttca ggtcttttgg tacctcgtta
    21061 agtgcttgct ctgcagattc ataatcagct gcgaagtatt cagcgttggc atttgcaaaa
    21121 tgacgcatga gtttgaagag gtgtgatggt gaataacgtg cagatggagt gtcagcccaa
    21181 gcagctacca taccaccgat gattgggata tcagctcctt ctgtttttgg tacagaagtg
    21241 attggtgtgt ttttaatacc attgagcccc tgatcgagat tgtaccagcc ttggccatca
    21301 gcgtttcgtc caagaacgta gtaccaagca tcattggtat taaggatttg gtgacctttt
    21361 tcagctagta gtttagaaga agcgacatcg tagcctcccc aaccaccagt ccacatagaa
    21421 acgatgatgt ctttgtcaaa actaccaaag cttgtgtcgc tattgtagta gataccgtcg
    21481 ttaaaggcca ttggtttgag accgtgcgat tttacaatac gagcgaggtc attggcgtag
    21541 gcaataaatt tttcatagcc ttttacaggg tagccttcgt ttggatagta tttatcagct
    21601 tgaagcacac tccaaccttt agcatctgtc gcatcattgg catattcatc aagtccgatg
    21661 ttgaagattt cagtcttttt cgcgaaataa gcagcatact tgtcgataag ggcttttgta
    21721 aaagcgacag cttgttcgtt gtcaagatcg acagtacggg ctgattcctt cccaaaatag
    21781 ctaaagttag ggttttggat tcccaattct ttcatggcat tgagaatcgc atccatgtga
    21841 ccaggactat ttactgtcgg aatgagaccg atacctttat cttttgcata gttaatcaga
    21901 tctgtcattt gactttctgt taagtgattg ccgtttggat cgttgtaata atcatttgta
    21961 cctttttcaa tggcgcgttt gacatcgtca ctggcatagg tcttgccgtt agctgtgatg
    22021 ctcatatcgt ccaacatgaa acggagtcca tcatttccga ctaataggtg taaatcagtg
    22081 tagccataat gtttcgcttt atcgatgatt tccttgagct gttctggtga gaaatattta
    22141 cgtccagcat caatagaaac aattttcttt ttcgctagtt tttcacttac agttgcagca
    22201 cgttcctttc ctgcctctgt tgccggtttg tcagcctctg ctttcgcttc atctttttta
    22261 gctggtttat cctttttagg gctagcttct tctgcctttt tattagcagt ttctttttca
    22321 gcagaagttg gagttaccac ttctgcttta tcactaggag ttgaactaac ttcctcttgt
    22381 ggtttttctt ctgtttttgg aagactagct accttatcag tagctggagt ttctgtttct
    22441 acagtttttg gagcttctgg ttgaagctct gctttaggtg tttcctcagt ccgattttcg
    22501 gatgattgag gggaatcaga aaccgtatgg atggtcggtt ggttttctgt agtagtagga
    22561 gtaactccat cggctgcaac agtctgtgct tggaaggcaa atccaattag aacagaagct
    22621 gctcctacag cgtatttacg aatagaaaaa cgctgttgtt tttcatgttt cattgcaaaa
    22681 cctcctgatt gcattgttat attgatagcg attatataaa tcaacgcctt tattttattt
    22741 cttgttttaa cgagagtcaa gaggagatga caaaaaacta taataagtat aaaaaaatat
    22801 aaaatttaaa cttaagattt cagattggtc gaaaaaatac atatatatat ctagtataat
    22861 ttttggttct atttctataa aatattccac aaattataga atcttccaaa aataggtaag
    22921 cgctaccttt ttggtgtggt ataataagca tagaaaaagc ccgagcgatt aggctcaggc
    22981 tttcttctta gtgatcacgg tcacatgaga tgaatttaat cttgtagtaa tcagatcgtt
    23041 tgtaagtttc actgtattct aaaacttggc cagttgattc gagtttggtg attttagttt
    23101 gtaggacagt agggaattgt tcatcgactc cgaggactga agccgcatgt tctggagttg
    23161 gaaagactat ttcgttgatt tcttcaaagt gttcatcatt catgtgaatg tggtagtcta
    23221 acttgaaacg attatagata gaactatagt attcaaggtt tggataattt gcgttgatat
    23281 attgttctgg gatgtaggat gtatggtaga tataaacgac accgtttgat tcgcggatac
    23341 gttcaatctt gtagtagaat tgatcgccgc gtagacccaa tttttccaag taaacaagct
    23401 tgtttccgcg ttcaattgaa agaacagtta ccttatcatc tttagcattg aagagttcaa
    23461 tatctgaaaa ctctacaagc ttgtgtttgc gtgcacgtga aacgaaggtt ccttttcctt
    23521 gttggcggac aatatagcca tctttggcaa ggtcgtttaa ggcgcgaaca actgtgatag
    23581 agctgacatc gtacattgaa atgagttctg cttcagtgta aaatttatct ccactgctaa
    23641 actgcccaga gatgatttta ttttttaatt cgtcttttat gtattgatat ttgggaattg
    23701 ccatattttt acctctattt tccttctcca tagaaaattt taccataaaa gcgaaaaaaa
    23761 tagtagtctt t
//
LOCUS       DAVKGC010000023        21179 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKGC010000023 DAVKGC010000000
VERSION     DAVKGC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21179
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.023"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(51..188)
                     /locus_tag="VW049_000715"
     CDS             complement(51..188)
                     /locus_tag="VW049_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431877.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDEISNHTYGKATLTWFEEI
                     F"
     gene            245..1294
                     /locus_tag="VW049_000716"
     CDS             245..1294
                     /locus_tag="VW049_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9431878.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLTAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(1394..5191)
                     /gene="spuA"
                     /locus_tag="VW049_000717"
     CDS             complement(1394..5191)
                     /gene="spuA"
                     /locus_tag="VW049_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9431879.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKSEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            5607..6020
                     /gene="rpsL"
                     /locus_tag="VW049_000718"
     CDS             5607..6020
                     /gene="rpsL"
                     /locus_tag="VW049_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9431880.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            6040..6510
                     /gene="rpsG"
                     /locus_tag="VW049_000719"
     CDS             6040..6510
                     /gene="rpsG"
                     /locus_tag="VW049_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9431881.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            6935..9016
                     /gene="fusA"
                     /locus_tag="VW049_000720"
     CDS             6935..9016
                     /gene="fusA"
                     /locus_tag="VW049_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9431882.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            9120..13511
                     /locus_tag="VW049_000721"
     CDS             9120..13511
                     /locus_tag="VW049_000721"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9431883.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMTPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVSRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            13613..13876
                     /locus_tag="VW049_000722"
     CDS             13613..13876
                     /locus_tag="VW049_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9431884.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            13869..14147
                     /locus_tag="VW049_000723"
     CDS             13869..14147
                     /locus_tag="VW049_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9431885.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            14168..14320
                     /locus_tag="VW049_000724"
     CDS             14168..14320
                     /locus_tag="VW049_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431886.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALREK"
     gene            14342..15583
                     /locus_tag="VW049_000725"
     CDS             14342..15583
                     /locus_tag="VW049_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9431887.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVGGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            15593..15823
                     /locus_tag="VW049_000726"
     CDS             15593..15823
                     /locus_tag="VW049_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9431888.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            16046..16768
                     /locus_tag="VW049_000727"
     CDS             16046..16768
                     /locus_tag="VW049_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9431889.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            16985..18319
                     /gene="pepC"
                     /locus_tag="VW049_000728"
     CDS             16985..18319
                     /gene="pepC"
                     /locus_tag="VW049_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9431890.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(18375..19286)
                     /locus_tag="VW049_000729"
     CDS             complement(18375..19286)
                     /locus_tag="VW049_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9431891.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(19310..20113)
                     /locus_tag="VW049_000730"
     CDS             complement(19310..20113)
                     /locus_tag="VW049_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9431892.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(20141..21130)
                     /gene="manX"
                     /locus_tag="VW049_000731"
     CDS             complement(20141..21130)
                     /gene="manX"
                     /locus_tag="VW049_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9431893.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         6018 a         4137 c         4962 g         6062 t
ORIGIN      
        1 ttttctaatg agcaaattgg ttgcttgtac ttaagattcg tgttatactc ttagaaaatc
       61 tcttcaaacc acgtcaacgt cgccttgccg taggtatggt tactgatttc gtcagttcta
      121 tccacaacct caaaacactg ttttgagcaa cctgcggcta gcttcctagt ttgctctttg
      181 attttcattg agtattagaa taatttttca aattgaagga cagagataga caaggagaat
      241 cacaatggta gaattgggaa tttcaacatt tggggaaata acggagcttg aagggactgg
      301 acaaacttac agtcatgctg aacgcattcg ccagttggtg gcagagattg agctggctga
      361 caaggttggt ttggacgtat atgggattgg cgagcaccat cgggcggatt ttgcagtatc
      421 agccccagag attgtcctga ccgctggggc agtcaatacc aagaaaatcc gtttgaccag
      481 tgcagtcagc attctgtcaa gcatggaccc gattcgtttg ttccaacagt atgccactat
      541 cgatgctttg tcaaatggac gagcggagat tatggctgga aggggctctt tcacggaatc
      601 tttccctctg tttggttatg acttgaaaga ctacgactct ctttttgatg agaaattaga
      661 cttgcttcag ttagtcaatg aaaagaccaa gttagactgg caaggtcgat tgactcaaac
      721 gattgctggt aaagaagttt atcctcgtcc agttcaggac aaattgccct tgtggatagc
      781 tacaggtggt catgtcgaat caacagtgaa gattgctcag gctggtctac cgattgtata
      841 tgctattatt ggtggtaatc cacgttattt taaaaagttg attcaggctt atcgtgagat
      901 tggaagcgaa gcgggccatg ctgacaaaga cttgaaagtg ggagcccatt cttgggggtg
      961 gattgcggaa gatggtgagc aggcggtgaa agattatttc catccgacta agcaagtggt
     1021 ggatgctatt tccaaagacc gtccgcactg gcaggaattg cgttatgagc aatatttgga
     1081 gcaggtaggg ccaaatggcg ccatgtttgt tggcaatcca gatcaggtgg ccgaaaaatt
     1141 gattcgcatg atagaggatt taggattgga ccgcttcatg cttcatctac cgcttggttc
     1201 tatgcctcat gaccaggttc tgagagctat tgaactcttc ggaacacaag ttgcacccaa
     1261 agtacgagct tattttgcta tgaaagaggc ttaataaaaa atcctaatca agtttattag
     1321 gacaacaaat attgtacaaa aattaatccc ccgagctata atgcttgggg gatttttcta
     1381 taggagggct agtttagttc tcttttttat tttttagtaa gaaaccgaga ccaaggagcg
     1441 caaggaggct gattcctgca aataggagtt tgttttcgtt tttgattcct gtatttggaa
     1501 gttcagcttg tttatctggg gttgcaggta tattttcctt gctagagttt tctaccgatt
     1561 cgtttcgaac cgcttctttt acagatgatg cttgtgcttc ttgtgctggt tgttcagctt
     1621 gtgaatcagc tgtagcttgg ctaggtttat tttccgcatc agctactttg gcatctggtt
     1681 tagtagaatc aggtctagct tctggagctg ggtcttgatg tgcagggtga gaagcttcat
     1741 tttgatgttc aggcttggaa ggggttgagt ctggtttctc ttctgcagtt gactcatggc
     1801 tagttccatt ttgagagact cgaagaacag tagctgtaag ggcattcaat ttcaagcctt
     1861 tttcagtcca ttcaagtcct ttcgggttgg caattccgac tgatcctgct tggttttcat
     1921 ctgccaaaac ttccgcattt cttagatgtg caaaggcagt tcccaaatta aattcgcgag
     1981 ctttttcatc cgcattgaca aagactgcgt agatatcgcc gtttggagca gtgatttggt
     2041 agccaatcac tacatcctct ttttccacac cattttggcc tgggacagtg atgaggtgga
     2101 cacggtcttt gatatcttgg agactcttaa gtcggaaggc atctgtagat tgacgaaggg
     2161 caatcaaacc tttcatatag tcacggctct tgacattttc aggataagct ttaccatctg
     2221 tagccttagt ccagtcaaac ttgttgactg cgtcgctaga atcgtaagaa tcatggatga
     2281 agtaaggata gtcaaatggg ttgccgtcct tatcacgcaa caagtgagat ttgtttggaa
     2341 ccttatcctc tgctactgga gtcttgtagg ctgggtcacg gaattgttta gtacgtccat
     2401 attcctgacc ggagtggata aatggagttc cttgagctgt caagaccatg agatttccaa
     2461 gtcgtaaacg acggtggatt tcagcatagt tctcagcctt gcttgggtct tttttgatag
     2521 actgggcaat gatgtcaaag agggtcaagt tatcatgggc tgcgatgtat tggatgacat
     2581 ctccagggct gtcagcttca aagttagttg gttgagcaat gagattttta aagatggtgt
     2641 tgacatcacg cttgccacct gtgataaagg caggttgacc ttcgtttgga taaccagatt
     2701 tgaggttgtt acggatgtca tctgaaaaga cagcgacagt atcggtatgt ttcatccaat
     2761 cttggtcagc agctttagta ggcatgtttt catcaccggc ataggttctc caaccttcac
     2821 caagcatgat gaggtttgga ttgagggcgc gtgcagcctt gtaagcttct tcgatagaag
     2881 cggcgtcatg gtctcccatc atatcgaagc ggaagccatc cactttgtag gtatcaacta
     2941 ggtatttgat agagtcaact aggagccgtt tggtcatatg gtgggttgtc cccaagcgtc
     3001 caccaccaaa gctagttcga ggtgtgccat cggcatccat aaagtggtag tagtttggct
     3061 ctaggtcttc aaagatatcg actttggctg tgtggttata aacgacatct aggatagctc
     3121 ccataccacg tttgtggatt tcgttgatga ggtttttaaa ttctgcgatt cgtttttctg
     3181 gattcttagg atcgcttgag tacataccag tcaaggagaa gtagttttga gggtcatatc
     3241 cccagttgta gttgctgttg cttgaagcgt agtcagacaa gcgttcatgg tttttcaatt
     3301 cattgacaaa gtagtaagac aagactggaa ggagctggat atgggttaca cccaagtctt
     3361 tgagatagtc tagtttttca atgaaggctt caaaagtccc aaatggtttg gtcaagtctt
     3421 ttgcaatggc aggatctgaa gtgaagtcac gcacatgagc ttcgtagata acggcgtctt
     3481 cacgagtctt gaaattgtga atcttaccat aagtcaagtc ttgaggtccg agtttagctg
     3541 gatctacaaa ggcggcttta gccactttat gggcatcgtc aattttggca tcgtcgctat
     3601 tccaagcagc aagagattta gcgtaaggat cgagtgcaag aacagtttta ccttgacgct
     3661 cgatttggta ttgataatag tagccagtga aatctgtgat tccgagtttg tttgtgctgt
     3721 ctagagtttg tttccaagtt cctctttccc ctttttcgag agcgacagtt ccaactactt
     3781 tgtcagggtc attcttgtcg tagacaacaa cagaaacctt atcagcactt ggtgaccaaa
     3841 gggtcaaatc aacttgtttt ccttcttctt ttaggtcagc tcccagtttg ccatcatagc
     3901 tgtatgtctc atctttcagg cgccagcttg ttttggtagt gaattggtcg gaattgtagc
     3961 taacagtata aggatgtttt gtgtcagaga aatctccgct gtaggtcact ttcttaccag
     4021 cttcatcgat tgcaacatcg gtaatagtta ccttgtttcc taggtgatta gtgatgttgg
     4081 agtgtttgag gatatcttct tttttagcac cgacaagtgt tgaaaagcta ctttcaatgc
     4141 tagaagtgcc tacgtgttgg gctcctgtca tacggatatc atggacatag tatggatttg
     4201 tgtaaatcga ttcatcatcg tcttttagga aaatttggct atgatttttc aaatctgtga
     4261 acttataatt ttctttacgg attttcacgt cgtctccttg tttgctctca tctagtaata
     4321 aaaatccaaa ttctcttgcg gcttcattaa gaggaatgtc gatatagcgg ccatatttgc
     4381 ctgtagccgt aaagtctgtt ccgtcaggcc attgagcgct acttggattt tttacatctc
     4441 cccagtacca gagagatttc ttgtcatagt tgccatctgt gcggtagtag ttgacgcgaa
     4501 cagttcctgc aggctgtggc tcgtaagaga aaaccttgta atcttggtct aaccaagcct
     4561 cgttcatttt tggaactagt ttttctacag atttatcgcc ggttagattt tttccagctg
     4621 tattgttgat gaggaagcta attttcttgg cttgttctcc ctttaatttg acatctaggt
     4681 aatagccgta gtcatctttc ttggcatcct tgaaggacaa agctccgttt ggccagtttt
     4741 cagatggttt ttcaacatcg tcccaagtcc atagtccttg agcatccttg ttttcttcag
     4801 gaagtttttt gacatggata cggaagtagt tatcttcgat gggctcttct gacttagttt
     4861 cagttgttac tggactagta gaagtagttg gctctgttgc aggtttttct tctgttttct
     4921 ctagggaagc gtttgcattt ctaggtgaag aaatatcact ttcgttctta ccagagccag
     4981 ctgctgtatc agtaagaggt tgggcaaggg tggttgtatt tctgttgttg ggttcgttag
     5041 tggttggtgt actttcgtcg gatgagatag ttggtgtagc catggcaagc aagacaaggc
     5101 ttgcgccgat aaggacagaa ccagttccat ttttgaggga acggatgctg tagaccattt
     5161 ttttctcagt gtgagatggt gtttttctca taatgattct ccaatcaata aatttctata
     5221 tcagtatagc atgtagtaaa acaatatgca agcgttttct ttgatttgtg aaaaaattct
     5281 tacttttgag aagttttctg tactatttta tatatcagta taaatgacgg agataaaaat
     5341 gtcacataag ccaagccgta ttattaggaa gatgatgtgt tcccagctat ttttattcag
     5401 aaattgatct ctaacattac caataaaaaa gaaaaatatc ttgacaagca agggaaaatt
     5461 ttgttacaat aatagacggt actttttact tttggtctct caagagtgta cagggacgtg
     5521 ctgacaaatg ttgcaaaagt acacacagat gatatctgtc accaagtgta tcatcaccaa
     5581 aaataaaaaa acacaggaga atgtagatgc ctacaattaa ccaattggtt cgcaaaccgc
     5641 gtaaatcaaa agtagaaaaa tctaaatcac cagctttgaa cgttggttac aatagtcata
     5701 aaaaagttca aacaaacgtt tcttcaccac aaaaacgtgg tgttgcaact cgtgttggaa
     5761 caatgacacc taaaaaacct aactcagccc ttcgtaaatt cgctcgtgta cgtttgagca
     5821 accttatcga agttactgcc tacatcccag gtatcggaca caacttgcaa gagcacagcg
     5881 tggtgcttct tcgcggtgga cgtgtaaaag accttccagg ggtacgttac catatcgtcc
     5941 gtggtgcact tgatactgca ggtgttaacg atcgtaaaca aggtcgttct aaatacggta
     6001 ctaaacgtcc aaaagcataa ggaaagggga taaagagaaa tgagtcgtaa aaatagagct
     6061 ccaaaacgtg acgtattgcc agatccgcta tacaattcac aactagttac tcgtcttatc
     6121 aaccgcgtta tgcttgatgg taaacgtggt actgctgctt caatcgttta cggtgctttt
     6181 gagcaaatca aagaagctac tggcaacgat gcacttgaag tatttgaaac agctatggaa
     6241 aacatcatgc ctgtacttga agtacgtgca cgtcgtgttg gtggttctaa ctaccaagtc
     6301 ccagttgaag ttcgtccaga acgtcgtaca acacttggac ttcgttggtt ggtaacaatc
     6361 gctcgtcttc gtggtgaaca cacaatgcaa gaccgtcttg caaaagaaat cttggatgct
     6421 gctaacaaca ctggtgcagc agttaagaaa cgtgaagata ctcaccgtat ggctgaagct
     6481 aaccgtgcat tcgcacactt ccgttggtaa gataggatgc gaaagcgtta agaaagtccc
     6541 agagaaaata gggaatcgaa gcaggttgcg gttgcaacca atgagattca tctttttctc
     6601 cagactttta gcttgagctc aactaaatca tgatgctagg aacggtaagg atgcaaggtg
     6661 aaaataggaa actgacgcag tattcgacga atacaaggag ttttatcttt ttcacgcagc
     6721 atcccgttcc agctcacatc ggctaactaa ctttagcccg ggttcaaatt agctaaatcg
     6781 attagtatta gctataactc agcttaccat ctcgtaagtt gaaaccaaca atagcatgaa
     6841 aacattgaga acgggtaggt cctgcctatc cgtttttatt aaaatcgtgt tataatagaa
     6901 tagaaatcaa aaataaatag gagaaacaaa cctcatggca cgcgaatttt cacttgaaaa
     6961 aactcgtaat atcggtatca tggctcacgt cgatgccggt aaaacaacaa ctactgagcg
     7021 tattctttac tacactggta aaatccacaa aatcggtgaa actcacgaag gtgcgtcaca
     7081 aatggactgg atggagcaag agcaagaacg tggtatcacg atcacatctg ctgcgacaac
     7141 agctcaatgg aacaaccacc gcgtaaacat catcgacaca ccaggacacg tggacttcac
     7201 aatcgaagta caacgttctc ttcgtgtatt ggatggtgcg gttaccgttc ttgactcaca
     7261 atcaggtgtt gagcctcaaa ctgaaacagt ttggcgtcaa gcaactgagt acggagttcc
     7321 acgtatcgta tttgccaaca aaatggacaa aatcggtgct gacttccttt actctgtaag
     7381 cacacttcac gatcgccttc aagcaaatgc acacccaatc caattgccaa tcggttctga
     7441 agatgacttc cgtggtatca ttgacttgat caagatgaaa gctgaaatct atactaacga
     7501 ccttggtacg gatatccttg aagaagacat cccagctgaa taccttgacc aagctcaaga
     7561 ataccgtgaa aaattgattg aagcagttgc tgaaactgac gaagaattga tgatgaaata
     7621 cctcgaaggt gaagaaatca ctaacgaaga attgaaagct ggtatccgta aagcgactat
     7681 caacgttgaa ttcttcccag tattgtgtgg ttcagccttc aaaaacaaag gtgttcaatt
     7741 gatgcttgat gcggttatcg actaccttcc aagtccactt gacatcccag caatcaaagg
     7801 tattaaccca gatacagacg ctgaagaaac tcgtccagca tctgacgaag agccatttgc
     7861 agctcttgcc ttcaagatca tgactgaccc attcgtaggt cgtttgacat tcttccgtgt
     7921 ttactcaggt gttcttcaat caggttcata cgtattgaat acttctaaag gtaaacgtga
     7981 acgtatcgga cgtatccttc aaatgcacgc taacagccgt caagaaatcg acactgttta
     8041 ctcaggtgat atcgctgctg ccgttggttt gaaagatact acaactggtg actcattgac
     8101 agatgaaaaa gctaaaatca tccttgagtc aatcaacgtt ccagaaccag ttatccaatt
     8161 gatggttgag ccaaaatcta aagctgacca agacaagatg ggtatcgccc ttcaaaaatt
     8221 ggctgaagaa gatccaacat tccgcgttga aacaaacgtt gaaactggtg aaacagttat
     8281 ctcaggtatg ggtgaacttc accttgacgt ccttgttgat cgtatgcgtc gtgagttcaa
     8341 agttgaagcg aacgtaggtg ctcctcaagt atcttaccgt gaaacattcc gcgcttctac
     8401 tcaagcacgc ggattcttca aacgtcagtc tggtggtaaa ggtcaattcg gtgatgtatg
     8461 gattgaattt actccaaacg aagaaggtaa aggattcgaa ttcgaaaacg caatcgtcgg
     8521 tggtgtggtt cctcgtgaat ttatcccagc ggttgaaaaa ggtttggtag aatctatggc
     8581 taacggtgtt cttgcaggtt acccaatggt tgacgttaaa gctaagcttt atgatggttc
     8641 atatcacgat gtcgactcat ctgaaactgc cttcaagatt gcggcttcac tttcccttaa
     8701 agaagctgct aaatcagcac aaccagctat ccttgaacca atgatgcttg taacaatcac
     8761 tgttccagaa gaaaaccttg gtgatgttat gggtcacgta actgctcgtc gtggacgtgt
     8821 agatggtatg gaagcacacg gtaacagcca aatcgttcgt gcttacgttc cacttgctga
     8881 aatgttcggt tacgcaacag ttcttcgttc tgcatctcaa ggacgtggta cattcatgat
     8941 ggtatttgac cactacgaag atgtacctaa gtcagtacaa gaagaaatta ttaagaaaaa
     9001 taaaggtgaa gactaatccg tcctcactct agaaggaagt cacttagtgg cttccttttg
     9061 tctttagaaa atacctctaa atatggtaaa atagtagaag aataatgtga ggaaaatgaa
     9121 tgtcaaatag ttttgaaatt ttgatgaatc aattggggat gcctgctgaa atgagacagg
     9181 ctcctgcttt agcacaggcc aatattgagc gagttgtggt tcataaaatt agtaaggtat
     9241 gggagtttca tttcgtattt tctaatattt taccgattga aatcttttta gaattaaaga
     9301 aaggtttgag cgaagaattt tctaagacag gcaataaagc tgtttttgaa attaaggctc
     9361 ggtctcaaga attttcaaat cagctcttgc agtcctacta tagggaagct ttctctgaag
     9421 gtccatgtgc tagtcaaggt tttaagtccc tttatcaaaa tttgcaagtt cgtgctgagg
     9481 gtaatcagct atttattgaa ggatctgaag cgattgataa ggaacatttt aagaagaatc
     9541 atcttcctaa tttagccaaa caacttgaaa agtttggttt tccaactttt aactgtcaag
     9601 tcgagaagaa tgatgtcctg acccaagagc aggaagaggc ctttcatgct gaaaatgagc
     9661 agattgttca agctgccaat gaggaagcgc tccgtgctat ggaacaactg gagcagatga
     9721 cacctcctcc agcggaagag aaaccagtct ttgattttca agcgaaaaaa gctgcagcta
     9781 aacccaagct ggataaggcg gagattactc ctatgatcga agtgacgaca gaggaaaatc
     9841 gtctggtatt tgaaggggtt gtttttgatg tggagcaaaa agtgactaga acaggtcgtg
     9901 ttttaatcaa ctttaaaatg acggactata cttcaagttt ttctatgcaa aagtgggtta
     9961 aaaacgagga agaggcccag aagtttgacc tcatcaagaa gaattcttgg ctccgagttc
    10021 gagggaatgt ggagatgaat aacttcacac gcgatttgac tatgaacgta caggatctgc
    10081 aggaagttgt tcactatgag cggaaggatt tgatgccaga aggtgagcgt cgggttgagt
    10141 ttcatgctca tactaacatg tcgactatgg atgctttgcc agaggtcgaa gagattgttg
    10201 caacagctgc taagtgggga cacaaggcgg ttgctatcac agaccatggg aatgtccagt
    10261 cctttccaca tggctataag gcggctaaga aagcgggaat ccagctgatc tatgggatgg
    10321 aagccaatat cgtggaggac cgtgtcccta tcgtctataa cgaagtggag atggacttat
    10381 cagaagcaac ctacgtggtc tttgacgtgg aaacgacggg actttcagct atctataatg
    10441 acttgattca ggttgcggcc tctaagatgt acaaggggaa tgttattgct gaatttgatg
    10501 aatttatcaa tcctgggcat cccttgtcag cttttactac agagttaact ggaattacag
    10561 atgatcatgt caaaaatgcc aaaccactag aacaagtttt gcaagaattc caagaatttt
    10621 gcaaggatac ggtcctagtt gcccacaatg ctacctttga cgttggcttt atgaatgcta
    10681 attatgagcg tcatgatctt ccaaagatta gtcagccagt tattgatacg ctggagtttg
    10741 ctagaaacct ctatcctgag tataaacgcc atggtttggg gcctttgacc aagcgttttg
    10801 gtgtggcctt ggaacatcac cacatggcca actacgatgc ggaagcgact ggtcgtctgc
    10861 ttttcatctt tatcaaagag gtagcagaaa aacatggtgt gaccgattta gctagactca
    10921 acattgatct aatcagtcca gattcttaca aaaaagctcg gatcaagcat gcgaccatct
    10981 atgtcaagaa tcaggtaggt ctaaaaaata tctttaagct ggtttccttg tctaatacca
    11041 agtattttga aggagtgtca cggattccga gaacggttct agatgcccat cgagagggct
    11101 tgattttagg ttcagcctgt tcagagggtg aagtttttga cgtggtcgtt tctcaaggtg
    11161 tggatgcggc ggttgaggtg gccaagtatt atgactttat cgaggtcatg ccaccggcta
    11221 tctatgcgcc cttgattgct aaagagcagg tcaaggatat ggaggaactc cagaccatta
    11281 tcaagagttt gatagaggtt ggagaccgcc ttggcaagcc tgttctggct acgggaaatg
    11341 ttcactatat cgaaccggaa gaagagattt atcgtgaaat tatcgtccgt agtttgggac
    11401 agggtgcgat gattaaccga actatcggtc atggtgaaca tgcccaacca gcaccacttc
    11461 caaaggctca ttttcgaacg actaatgaga tgttggatga atttgccttt ttgggagagg
    11521 aactggctcg taaactggtt attgaaaaca ccaatgcctt ggcagaaata tttgaacccg
    11581 ttgaagtcgt taagggtgac ttgtatacgc ctttcatcga caaggctgaa gaaacagttg
    11641 ctgagttgac ctataagaaa gcttttgaga tttatggaaa tccgctgcca gatattgttg
    11701 atttgcggat tgaaaaagaa ttaacatcca tactggggaa tggatttgct gtgatttatc
    11761 tggcatcgca gatgctggtg caacgttcta atgaacgggg ttatttggtt ggttctcgtg
    11821 ggtctgtcgg atctagtttc gttgcgacca tgattgggat tacggaggtc aatcctctct
    11881 ctcctcacta tgtctgtggt cagtgtcagt acagtgagtt tatcacagat ggttcgtacg
    11941 gttcaggatt tgatatgccc cataaggact gtccaaactg tggtcacaaa ctcagtaaaa
    12001 acggacagga tattccgttt gagaccttcc ttggttttga tggggataag gttcctgata
    12061 ttgacttgaa cttctcggga gaagatcagc ctagcgccca cttggatgtg cgtgatatct
    12121 ttggtgaaga atatgccttc cgtgcgggaa cggttggtac ggtagctgcc aagactgcct
    12181 atggatttgt caagggttac gagcgagatt atggcaagtt ttatcgtgat gcagaagtag
    12241 aacgcctcgc tcaaggagcg gcgggtgtca agcggacaac aggccaacac ccggggggaa
    12301 tcgttgttat tccgaactat atggatgtct acgattttac gcctgtccag tatccagcag
    12361 atgatgtcac ggctgaatgg cagaccactc actttaactt ccacgatatc gatgagaacg
    12421 tcctcaaact cgatgtactg gggcatgatg atccgaccat gattcgaaaa cttcaggatt
    12481 tgtctggtat tgaccctaat gaaattccta tggatgacga aggcgtgatg gcactctttt
    12541 ctgggactga tgtgctaggg gtaacacctg aacaaattgg aacgcctacg ggtatgttgg
    12601 ggattccaga gtttggaaca aatttcgtac gtggaatggt agacgaaacc catccgacaa
    12661 cctttgcgga attgcttcag ctgtctggtc tgtcccacgg tactgacgtt tggttgggga
    12721 atgctcagga tctgattaag caaggaatag cggacctatc gactgttatc ggttgtcggg
    12781 acgacatcat ggtttacctc atgcatgcgg gtctggaacc taagatggcc tttaccatta
    12841 tggaacgggt acgtaagggt ttgtggctaa agatttcaga agaggagaga aatgactata
    12901 tcgaagccat gaaggctaat aaggtgccag agtggtatat cgaatcctgt gggaaaatta
    12961 agtacatgtt ccctaaggcc catgcggcag cctacgttat gatggccttg cgtgtagctt
    13021 acttcaaggt tcaccatcct atttattact actgtgctta cttctccatt cgtgctaagg
    13081 cttttgatat caagaccatg ggtgcgggct tggaggtcat caagcgcaga atggaagaaa
    13141 tctctgaaaa acggaagaac aatgaagcct ctaatgtgga aatcgatctc tatacaactc
    13201 ttgagattgt caatgagatg tgggaacgag gtttcaagtt tggtaaatta gatctctact
    13261 gtagtcaggc gacagagttc ctcatcgacg gggataccct tatcccacca tttgtagcaa
    13321 tggatggtct gggagagaac gttgccaagc aactggtgcg ggcgcgtgaa gagggagaat
    13381 tcctctctaa aacagaacta cgcaagcgtg gtggactctc atcaaccttg gttgaaaaga
    13441 tggatgagat gggtattctt ggaaatatgc cagaggataa ccagttgagt ttgtttgatg
    13501 agttgtttta aaaaattgct taataatcta ttaaaagagg ctaacgtata tccaatagat
    13561 ttacattagc tttctttttt gttaaaatag tctatggaaa gagggtgaga gtatgtcaaa
    13621 gatgagtata agcatccgtc tggatagtga ggttaaggag caggcccaac aggtgtttag
    13681 taatctggga atggatatga caacagctat taatattttc cttcgtcagg caattcaata
    13741 tcagggatta ccttttgatg ttagactaga cgaaaatcgg aagttgctcc aagtgttaac
    13801 ggatttagac caaaatcgta atatgagcca gtcttttgaa tcagtctcag atttgatgga
    13861 ggacttacgt gcttaagatt cgttatcata aacagtttaa aaaagatttt aagttggcta
    13921 tgaagcgtgg tttgaaggca gaattattag aagaagtttt gaattttctg gttcaagaaa
    13981 aagaacatcc tgccagatat cgtgatcatt cattgacggc atccaagcat tttcaaggag
    14041 ttcgtgaatg ccatacccag ccagattggc ttttggttta taaagtagac aagtcggaat
    14101 tgattttaaa tttgctgagg acaggcagtc acagtgattt attttaatct attttaaggg
    14161 ggttctcatg aaactaagaa tatttgcgga agataagccg gctaagaagg tatttgaata
    14221 tcaattagaa cttgctgatc gtacaattct tctatcgaca gcactcttgt caggtgctat
    14281 tgctttagca ggaatctttt ctgctttgag agaaaaataa aaatagaaaa gagaaaacag
    14341 aatggtttta ccaaatttta aagaaaatct agaaaaatat gcgaaattgt tggttgcgaa
    14401 cggaattaac gtgcaacctg gtcacacttt ggctctctct attgatgtgg agcaacgtga
    14461 attggcacat ctaatcgtga aagaagctta tgccttgggt gcgcatgagg tcatcgttca
    14521 gtggacagat gatgtgatta accgtgagaa attcctccat gccccgatgg agcgtttgga
    14581 caatgtgcca gaatacaaga ttgctgagat gaactatctc ttggagaata aggctagccg
    14641 tcttggagtt cgttcatctg atccaggtgc cttgaacgga gtggacgctg acaagctttc
    14701 agcttctgct aaagctatgg gacttgccat gaagcctatg cgtatcgcaa ctcaatctaa
    14761 caaggttagc tggactgtag cagctgcagc aggacttgag tgggctaaga aagtcttccc
    14821 aaatgctgcg agcgacgaag aagcagttga tttcctttgg gaccaaattt tcaaaacttg
    14881 ccgtgtctac gaagcagatc ctgttaaggc ctgggaagag catgcagcta tcttgaagag
    14941 caaggccgat atgcttaata aggagcaatt ttcagccctt cactacacag cgccaggaac
    15001 agatttaaca cttggtttgc caaagaacca cgtttgggaa tcagctggtg ctgtcaatgc
    15061 acagggcgaa ggattcttgc caaatatgcc gacagaagag gtcttcacag cgcctgactt
    15121 ccgtcgtgca gatggttatg tcacttctac aaaaccgctt agctacaacg gaaatatcat
    15181 cgaaggtatt aaggtaacct ttaaggatgg acaaatcgta gatatcactg ctgagaaggg
    15241 tgatcaggtt atgaaagacc ttgtctttga aaatgcgggt gcgcgtgcct tgggtgaatg
    15301 tgccttggta ccagatccaa gtccaatttc tcagtcaggc attaccttct ttaacaccct
    15361 tttcgatgaa aatgcgtcaa accacttggc tatcggtgca gcctatgcga ctagcgtcgt
    15421 tggtggagca gagatgagtg aagaagagct tgaagctgcg ggacttaacc gttcagatgt
    15481 tcacgtggat tttatgattg ggtctaatca gatggatatc gatggtatcc gtgaggatgg
    15541 aactcgagta cctcttttcc gtaatgggaa ttgggcaaat taaggagata atatgttagg
    15601 aagtatgttc gttggtctcc tagtgggatt tttagcaggt gctatgacca atcgtggaga
    15661 gcgaatggga tgttttggaa aaatgtttct cggttggatc ggagcctttc taggtcactt
    15721 gctctttgga acttgggggc cagttttatc aggaacagct attatcccag ctgttttagg
    15781 tgccatgatt gtcttagcta ttttttggag acgaggaagt taatttccta aatctgatac
    15841 tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag gttgctcaaa gcactgcttt
    15901 gaggttgcag atagaactga cgaagtcagt aacatatata cggcaaggcg acgttgacgc
    15961 ggtttgaaga gatttttgaa gagtatgaaa cgaaaaggag gttggtcatt gtactagcct
    16021 ccttttgata tgatataata gttctatgag attagataaa tttttagttg cctgtgcggt
    16081 gggaagtcgg actgaggtca aaaacttgct caaggctggg cgcgtgactg taaatggtaa
    16141 aaaagaaaaa tcagctaaat tgcagattga tgaaaaaata gatgagattc gctttgatgg
    16201 gcaagtgttg gagtatgaag agtttgtcta ctacatgatg aacaagccca aaggagttat
    16261 ctcagcgact gaggatccca agcacagaac cgttctggac ttgttggatg acttggcgcg
    16321 gagtaaggaa gttttcccag taggacgctt ggatattgac acgcatggtc ttttgctctt
    16381 gaccaatgat ggtcagctgg ctcatgttct tctttcgccc aagcgtcatg tggacaagac
    16441 ttatctggca caagtcaagg gaatcatgac ccaagaagat gtggagacat ttgctgaggg
    16501 tattcctctc aaagacttta cctgtcaacc cgctatactg gagcttgtat ccatagatac
    16561 agaaaagaat caaagccaaa tccgtgtgac cattgcagaa gggaagtttc atcagatcaa
    16621 gcgtatggtg ggctactgtg gcaaggaagt ggtggacttg caacgattga ctatgggaac
    16681 tttagtatta gatgagaacc tggaacgagg ggaatggcgt cgcttgacca aggaagaatt
    16741 agaaattctc cgcgctaata ttatttaatt tagtttaata ctcaatgaaa atcaaagagc
    16801 aaactaggaa actagccgca ggctgtactt gagtacggca aggcgacgtt gacgtggttt
    16861 gaagagattt tcgaagagta taaattcgaa aaaggtgagg gacaatgtcc tcgcctttta
    16921 tgttttttag ttgcttcctt tgtgaaaaga gttataatag actgtagaat aaaaggagga
    16981 atctatgaac gcgattcaag aatcatttac tgataaacta tttgccaact atgaagcaaa
    17041 tgtaaaatac caagcgattg aaaatgctgc cagccacaac ggaatttttg cagctctgga
    17101 acgtcgccaa agccatgtag acaacacacc tgttttctca ttggatttaa ccaaggacaa
    17161 ggtcactaac cagaaagcgt ctggtcgttg ctggatgttt gcggctctca acaccttccg
    17221 ccacaaactc atctcgcaat acaaattgga gaactttgag ttgtcacaag cccacacttt
    17281 cttctgggac aagtatgaga aatcaaactg gttcttggag caagtcattg cgacttcaga
    17341 ccaagaattg actagccgca aggttagctt cttactccaa acacctcaac aagatggcgg
    17401 tcaatgggat atggtcgttt ccctctttga aaaatacggt gtcgtgccta agtcagttta
    17461 tcctgagtct gtttcatcta gcagcagtcg tgagctaaat gcgatcctta ataaattgct
    17521 tcgtcaagat gctcaaatct tgcgtgattt gcttgtttct ggtgcagatc aagcgactgt
    17581 tcaagctaag aaagaagacc tcttgcaaga aatctttaac tttcttgcta tgtcattagg
    17641 acttccacca cgcaagtttg actttgctta tcgcgataaa gataacaact acaaaagtga
    17701 aaaaggaatc acaccacaag agttttacaa gaaatatgtc aatcttcctt tagaagacta
    17761 cgtttctgtt atcaatgctc caactgctga taaaccttac ggaaaatctt acacagttga
    17821 gatgttgggg aatgtggttg gtagccgtgc agttcgctac atcaacgttc caatggagcg
    17881 cttgaaagaa ttggcgattg cccaaatgca agcaggtgag actgtttggt ttggttctga
    17941 tgtcggccag ctcagcaacc gtaaggctgg catccttgcg acagatgttt atgactttga
    18001 atcaagcatg gacattaaac ttactcaaga caaggctgga cgtttggact atagtgaaag
    18061 cttgatgacc cacgccatgg tcttgacagg tgttgacttg gacgaaaatg gtaaatcaac
    18121 caagtggaag gttgaaaact catggggaga caaggttggt acagatggtt actttgttgc
    18181 ctcagacgct tggatggacg aatacacata ccaaatcgtt gttcgtaagg aattgctgac
    18241 agcagaagaa caagctgcct atggagcaga accaatcgta cttgcaccat gggatccaat
    18301 gggagccttg gctgaataaa agcatagaaa aaaggaatca gattttagaa cctggttcct
    18361 ttttagttgc ttgattacat gatgtgaaga acatgtgcca caatacccac tgcgaagagg
    18421 gcaaggataa tagtgattgg agatactttt ttcttaagta agtacatgca aagtaaagta
    18481 aggagtagtc ctgataatcc aggaatcaac atatccaagt tttgttggaa agtagtaact
    18541 ttttcaggag tttgagacaa tccttgtcct acttgtgcga atgcttcttg gataccttta
    18601 gacccttctg gcaatttatc ccaatggata taagcctttt catctagttg aactttagaa
    18661 acatcgaaag caaatttaat atttacccaa cgttgaacaa ggacagcaag aatgaacatc
    18721 ccaaggatag aagctccttt agtgatatct tgaaggatac caccagacat atctttagtg
    18781 atttctgatc cagccttgta tccaatctct tgaacatacc acaagaatga catacgaatc
    18841 aagttccatg caacaaagaa gaggagtggc cccaagatat tgccagtaag ggcaagtgaa
    18901 gcaccgagag atccaaggat tgggcgtact gtaaaccaga atactgggtc accgatacca
    18961 gcaagaggtc ccatcatacc gattttaacc ccttggatag cagcgtcatc gatttccaca
    19021 ccgttagcac gttcttcttc aagcgcaaga gtaaccccca tgactggagc agctacgtat
    19081 ggatgagtgt tgaagaactc aaggtgacgc tcaagagcag cgatttgatc ttctttttta
    19141 gtatagagtt ttttgatagc tggaatgagt gtataagccc agcccaagtt ttgcatccgt
    19201 tcaaagttcc aagacccttg taagaaggtt gaacgccacc aaactttttt acgatctgat
    19261 ttagttaatt gaagtttttc agtcatgatg ttttcagtcc tttcttatct tagtagtctt
    19321 ctaggatatc gccgattggg tcgttagaag ttgcggctcc tccgccacca tttccaccag
    19381 ttttagaaag gtgaaggtag ataagagcga tagcaacacc gatagcaccg aatccgatta
    19441 gagtaatatc tgacacagca gcgagaacga aaccaagagc gaagaatggc catacttcac
    19501 gagttgccat catgttgata accatggcgt aaccaacggc aacgaccata ccaccaccga
    19561 tagccatacc atctttgagc cagtctggca tggcactaag gatactttgt acagtttcag
    19621 ttggtaccat aaggagaaga gctgcaggaa gcgcgatacg aagtccttgg aaaagtagcg
    19681 caatgaaatg agcacgctcc acagcgccga agtcaccttt tttagcggca gcatcagcag
    19741 tgtgaaccaa tccaactgaa attgtacgaa caatcatagt taagaataga ccagctacgg
    19801 caagagggat agcaaccgct tgggcaacac cgataccagt cttggtaaag tcaccaccaa
    19861 gaaccatgat aatggcagca gcgacagaag caagtgcagc atcaggagcg atagcagcac
    19921 cgatatttga ccaaccaagg gcaatcattt gaagcgatcc accgaggata atccctgctt
    19981 ccaagtgacc tgttacaagc ccaataaggg tacaggctac aagtggttgg tgaaattgga
    20041 actggtcgag gatgccttca agacctgcaa agaaggctac aacgactact aaaaccatag
    20101 aaataataga catgtttaaa atcctttcat aaataatggc ttatttgaca ttggctttgt
    20161 taatcaagtc aaacaaatct tttttagaat catttggtac tttacggaca tcaaattcaa
    20221 cacccaagtc acgcattttt tcaaatgtag caacatcttc tttgtccata gacaaaacgg
    20281 tattgaccaa tgttttacct gttgagtgag ccatagaacc aacgttaaga gtcttgattg
    20341 gcacgccgcc ttcgatggca cgaagggcat cttgaggtgt ttcaaacaag ataagggcat
    20401 gtgtttctcc aaaacgtggg tcttttgaaa tctcaatcag tttttgaatt ggaaccacgt
    20461 tagccttgac attacctgga gctgcttgtt taatcaattc tttacgaagg tcgtctttag
    20521 ccacgttatc tgaagcaacg atgatacgat ttgcttttga atctggagtc caagcagttg
    20581 caacctgacc gtgaagtaga cgtgtgtcaa gacgggcaag attgattttc aatttaccgt
    20641 ctccgataac agttccttct gggatagcag tttgggcaac tggagcagct gcagcgcttg
    20701 caacttcttc aactggattt agctcttctg gaagagcttt gatgccatct ttggcttctt
    20761 taatgatatt agcagcgact ttttctacac ctgcagcagc gtccatgagg cgctctgtgt
    20821 aggcttgaat caacatcggt aagttaagtc ctgtgatgat ggcaaactta cgctcaggat
    20881 tttctcccat cacgcgacta gcttggttaa atggagaacc actccaaagg tcagccaaaa
    20941 ctagaacctc atcttctgcg tcaaatgcag caacagcgtt attaaactta gcgtatagat
    21001 catcaggacc ttcatttggc ataaaggtca caacttgaac cttttcttgt tcaccaaaga
    21061 tcatagatcc tgactgatga atacccgcag caaattcgcc gtggctcgca ataatgattc
    21121 cgatactcat tattgtcatt cctccttttt tgttttagtt ttcatttaag aaaacttta
//
LOCUS       DAVKGC010000024        21128 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKGC010000024 DAVKGC010000000
VERSION     DAVKGC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21128
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.024"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(30..1367)
                     /locus_tag="VW049_000732"
     CDS             complement(30..1367)
                     /locus_tag="VW049_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9431894.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(1377..1643)
                     /locus_tag="VW049_000733"
     CDS             complement(1377..1643)
                     /locus_tag="VW049_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9431895.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(1908..2294)
                     /gene="rplQ"
                     /locus_tag="VW049_000734"
     CDS             complement(1908..2294)
                     /gene="rplQ"
                     /locus_tag="VW049_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9431896.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(2306..3241)
                     /locus_tag="VW049_000735"
     CDS             complement(2306..3241)
                     /locus_tag="VW049_000735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9431897.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(3284..3667)
                     /gene="rpsK"
                     /locus_tag="VW049_000736"
     CDS             complement(3284..3667)
                     /gene="rpsK"
                     /locus_tag="VW049_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9431898.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(3685..4050)
                     /gene="rpsM"
                     /locus_tag="VW049_000737"
     CDS             complement(3685..4050)
                     /gene="rpsM"
                     /locus_tag="VW049_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9431899.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(4068..4184)
                     /gene="rpmJ"
                     /locus_tag="VW049_000738"
     CDS             complement(4068..4184)
                     /gene="rpmJ"
                     /locus_tag="VW049_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9431900.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(4209..4427)
                     /gene="infA"
                     /locus_tag="VW049_000739"
     CDS             complement(4209..4427)
                     /gene="infA"
                     /locus_tag="VW049_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9431901.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(4544..5182)
                     /locus_tag="VW049_000740"
     CDS             complement(4544..5182)
                     /locus_tag="VW049_000740"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9431902.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQNINDVFSDIEKVLTNLK"
     gene            complement(5333..6643)
                     /gene="secY"
                     /locus_tag="VW049_000741"
     CDS             complement(5333..6643)
                     /gene="secY"
                     /locus_tag="VW049_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9431903.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(6656..7096)
                     /gene="rplO"
                     /locus_tag="VW049_000742"
     CDS             complement(6656..7096)
                     /gene="rplO"
                     /locus_tag="VW049_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9431904.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(7241..7465)
                     /gene="rpmD"
                     /locus_tag="VW049_000743"
     CDS             complement(7241..7465)
                     /gene="rpmD"
                     /locus_tag="VW049_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9431905.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(7437..7931)
                     /gene="rpsE"
                     /locus_tag="VW049_000744"
     CDS             complement(7437..7931)
                     /gene="rpsE"
                     /locus_tag="VW049_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9431906.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(7949..8305)
                     /gene="rplR"
                     /locus_tag="VW049_000745"
     CDS             complement(7949..8305)
                     /gene="rplR"
                     /locus_tag="VW049_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9431907.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(8389..8925)
                     /gene="rplF"
                     /locus_tag="VW049_000746"
     CDS             complement(8389..8925)
                     /gene="rplF"
                     /locus_tag="VW049_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9431908.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(9117..9515)
                     /gene="rpsH"
                     /locus_tag="VW049_000747"
     CDS             complement(9117..9515)
                     /gene="rpsH"
                     /locus_tag="VW049_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9431909.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(9800..10069)
                     /gene="rpsN"
                     /locus_tag="VW049_000748"
     CDS             complement(9800..10069)
                     /gene="rpsN"
                     /locus_tag="VW049_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9431910.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(10087..10629)
                     /gene="rplE"
                     /locus_tag="VW049_000749"
     CDS             complement(10087..10629)
                     /gene="rplE"
                     /locus_tag="VW049_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9431911.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(10653..10958)
                     /gene="rplX"
                     /locus_tag="VW049_000750"
     CDS             complement(10653..10958)
                     /gene="rplX"
                     /locus_tag="VW049_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9431912.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(11036..11404)
                     /gene="rplN"
                     /locus_tag="VW049_000751"
     CDS             complement(11036..11404)
                     /gene="rplN"
                     /locus_tag="VW049_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9431913.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(11430..11690)
                     /gene="rpsQ"
                     /locus_tag="VW049_000752"
     CDS             complement(11430..11690)
                     /gene="rpsQ"
                     /locus_tag="VW049_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9431914.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(11715..11921)
                     /locus_tag="VW049_000753"
     CDS             complement(11715..11921)
                     /locus_tag="VW049_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9431915.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(11931..12344)
                     /gene="rplP"
                     /locus_tag="VW049_000754"
     CDS             complement(11931..12344)
                     /gene="rplP"
                     /locus_tag="VW049_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9431916.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(12348..13001)
                     /gene="rpsC"
                     /locus_tag="VW049_000755"
     CDS             complement(12348..13001)
                     /gene="rpsC"
                     /locus_tag="VW049_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9431917.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(13014..13358)
                     /gene="rplV"
                     /locus_tag="VW049_000756"
     CDS             complement(13014..13358)
                     /gene="rplV"
                     /locus_tag="VW049_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9431918.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(13370..13651)
                     /gene="rpsS"
                     /locus_tag="VW049_000757"
     CDS             complement(13370..13651)
                     /gene="rpsS"
                     /locus_tag="VW049_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9431919.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(13755..14588)
                     /gene="rplB"
                     /locus_tag="VW049_000758"
     CDS             complement(13755..14588)
                     /gene="rplB"
                     /locus_tag="VW049_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9431920.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(14606..14902)
                     /locus_tag="VW049_000759"
     CDS             complement(14606..14902)
                     /locus_tag="VW049_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9431921.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(14902..15525)
                     /gene="rplD"
                     /locus_tag="VW049_000760"
     CDS             complement(14902..15525)
                     /gene="rplD"
                     /locus_tag="VW049_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9431922.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(15550..16176)
                     /gene="rplC"
                     /locus_tag="VW049_000761"
     CDS             complement(15550..16176)
                     /gene="rplC"
                     /locus_tag="VW049_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9431923.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(16393..16701)
                     /gene="rpsJ"
                     /locus_tag="VW049_000762"
     CDS             complement(16393..16701)
                     /gene="rpsJ"
                     /locus_tag="VW049_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9431924.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(16967..17584)
                     /locus_tag="VW049_000763"
     CDS             complement(16967..17584)
                     /locus_tag="VW049_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9431925.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTSAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTAGLPVAHELESLQRDILALQASMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(17581..18171)
                     /gene="nrdG"
                     /locus_tag="VW049_000764"
     CDS             complement(17581..18171)
                     /gene="nrdG"
                     /locus_tag="VW049_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9431926.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELSDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(18164..18670)
                     /locus_tag="VW049_000765"
     CDS             complement(18164..18670)
                     /locus_tag="VW049_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9431927.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            complement(18714..18854)
                     /locus_tag="VW049_000766"
     CDS             complement(18714..18854)
                     /locus_tag="VW049_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431928.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKYSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(18866..21073)
                     /gene="nrdD"
                     /locus_tag="VW049_000767"
     CDS             complement(18866..21073)
                     /gene="nrdD"
                     /locus_tag="VW049_000767"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9431929.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLVDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
BASE COUNT         5798 a         4729 c         3989 g         6612 t
ORIGIN      
        1 aaatagaact taaaatttct cctattttct taatttttaa aactatgaat tggtgctggg
       61 atttgtccac cgcgagagat gaagtcgaca gacgaagccc cattaacctt cataacgggt
      121 gcagttccta atagaccacc aaactcaatc atatcgcctt cttttccttt gggaatgata
      181 cgaacagctg ttgttttcat gttgataaca ccgattgctg cttcatccgc aatcatagcc
      241 gcaatggttt cagcaggcgt atcttctggg atggcaatca tatccaatcc aacagaacag
      301 atagccgtca tagcttctag tttttctaaa ttaagagagc cattttgcac tgcagcaatc
      361 attccttcat cctcagaaac agggataaag gcaccagata aaccaccgac ttggttgcag
      421 gccatcactc cacccttttt aacttggtcg ttcaagaggg ccaaggcagc cgtcgttcca
      481 tgcgtgccaa ctgtttctag ccccatttcc tcaaggacac gtgccacaga gtctccaacc
      541 gcaggggttg gtgccaaact caagtccaca ataccaaact ccacacccag tctctcactg
      601 gccatttgac caaccaattg accgatacga gtgattttaa aggcagtttt cttaactgtt
      661 tcggctacta catcaaagct ctgtccacga actttttcca aagcacgttt cacaacacca
      721 ggaccagaaa ctccgacatt gatgataaca tctgcttccc caacaccatg aaaggcaccc
      781 gccataaatg gattgtcctc aacagcatta gcgaatacaa ccaacttggc cactcccata
      841 tctgaaagat ttgctgtttc cttgataatt cgtcccatat ctgccacagc cgtcatatta
      901 ataccagact tggttgagcc gatattgact gacgagcaga ccttatccgt ctcagccaaa
      961 gcgcgaggaa tggaattgat gagaatctca tctccctttt gataaccttt ttgtactaag
     1021 gcagaaaaac caccaataaa gtccacacca atctctttcg cagccttatc aagcgctttt
     1081 gccagaacca cgtagtccgt cgcatctgtc gctgccccaa tcagagaaat aggtgtcacc
     1141 gatacacgct tattaacgat aggaattccc aactcagccg caatttcatc accaacagct
     1201 actaaattag ccgcctttgt cgtaattttt tgatagattt tctccgcagc acgattgata
     1261 tctggatcga tacagtccaa aagagaaatc cccatggtaa tggttctaat atcgaagttt
     1321 tgctcctcaa tcatggcgat ggtttcagta acttgtctaa tatccatgat gacctcctag
     1381 atattataca tagcttcgaa aatcgctgca ctctgaatat tgatttttac attcaaagtt
     1441 tgcccaaaag cttcaaattc attacgaaga taggtaaaat cttgcttttc atcactagat
     1501 acaacagcca tcatcgtaaa atattcatcc aagacagttt gagagatatc gtcaatattc
     1561 aatcctaatt ctgcaatttt accagaaaca cctgcaacaa ttccagattt atctttacca
     1621 acaacagtta taatggcttt cataagtaaa ctccaattct tcttaaaata agaaacctta
     1681 acattaaaca ggattctttt caaatagtat agcataattc taacgaaaat actcaaaaac
     1741 gcctgcttac gaagttgttc ttaagaaaaa aacaatttcg taaacaagcg tctactatcc
     1801 caacttatga tgagtgttcc cgctaggacc gaaatcctag cggtagacca gagctagact
     1861 aagagcacaa gactccatca tcataacact caacaaaatt gatgatttta tactaattcg
     1921 atgatcgcca ttggcgctgc atcaccacga cgtggttcag ttttaaggat acgagtgtat
     1981 ccaccgttac gttcagcata acgaggtgcg atttctgaga acaatttttg aagtgctgta
     2041 gtagaagtgt acttatcagt tgcttcatca tagttttcag atgcgatttc attacgtacg
     2101 aaagcagctg cttgacgacg tgcatgcaaa tcaccacgtt tacctagagt aatcattttt
     2161 tcaacagttt tacggatctc tttagcacga gcttcagttg tcacgattga ttcgttgatc
     2221 aaaaggtcag ttgtcaaatc gcgaagcatt gctttacgtt gtgagctagt gcgtcctagt
     2281 ttacggtaag ccatgtattc ctcctttatt tatcttttaa tccaagaccc aaatcaatga
     2341 gtttgagttt cacttcttcc aaactcttgc gtccaagatt tcgtactttc atcatctctg
     2401 cttcagattt ttctgtcaaa tcatgcacag tattgatacc ggcacgtttt aaacagttgt
     2461 atgaacgcac agacaagtcc agttcctcaa tcgtacgatc taaaatacgg tcgtcagatt
     2521 cagtatcagc ttctttcatc acttcagttg acttagcaat ctcagtaaga tttgtaaaca
     2581 aatcaagatg ttctgtcaaa atacgtgctg aaagccctaa agcatcttct ggaataattg
     2641 ttccatttgt caagatttca agggttaatt tgtcgaaacc atcattgcta cctacacgag
     2701 caggttccac ttgatagttg acttttgtaa ctggtgtata aatagaatct acagcaagtg
     2761 ttccaactgg tgcattatcc tttttatttt catcagcagg tacatatcca cgaccactgt
     2821 taacagtcat agtcgctttt agagaagaac cttcaccgat tgtaaagaga taatgatctg
     2881 gatttacaat ttcaatatcg ctatctgtca aaatgtcacc agctgttact tcagcaggac
     2941 cttcaacatc cagttcgatg attttttcgt cttcaacgta cgatttcact gcaattcctt
     3001 taatgttcag aatgatttgc atcacgtctt cacgaacacc tggaactgtg tcaaactcat
     3061 gtaacacacc atcaatgttg atagatgtca cagctgctcc tggtagagaa gctagaagta
     3121 cacgacgaag agagttacca agagttgtac cgtagccacg ttcaagtggt tcgattacaa
     3181 acttgccata atctttattt tcatcaattt ttgttatatt tggtttttca aactcgatca
     3241 tttagttact ccctcttaaa cgaaaagcag tgtaatgcga tgattataca cggcgacgtt
     3301 ttggaggacg agcaccattg tgtggcactg gagtcacatc acgaattgct gttacttcaa
     3361 gaccagcggc agcaagcgca cgaatagctg actcacgacc agaacctgga ccttttacag
     3421 taacttcaac tgatttaaga ccgtgttctt gtgcagattt agcagcagct tcagaagcca
     3481 tttgagcagc gaatggtgta gatttacgag aacctttgaa accaagagca ccagctgatg
     3541 accaagcaat tgcattacca tgcacatcag taatcataac aatagtgtta ttaaatgtag
     3601 cgtgaatatg agcaatacca gattcgatat tctttttcac acgacgttta cgtgttggtt
     3661 tagccaagac ttttacctcc tatattattt tttcttacca gcaatcgcaa cagctttacc
     3721 tttacgagtg cgagcgttgt ttttagtgtt ttgtccacgg acaggaagtc cacgacggtg
     3781 acggatacca cggtatgaac cgatttccat caaacgtttg atgttcaagt ttacttcacg
     3841 acgaaggtca ccttcaactt tgattgcatc cacttcacga cggatagcat cttcttgatc
     3901 tgatgtaaga tcacgtacac gaacatcttc tgagattcca gcagcagcca aaattttctt
     3961 agatgttgca agtccgatac cataaacata agtcaatgag attactacgc gtttgtcatt
     4021 tggaatatca actccagcaa tacgagccat gttttctcct ttctatctta tccttgacgt
     4081 tgtttgtgtt ttggatttgc tgggcaaatt accataacac gaccattacg acgaataact
     4141 ttacagtatt cgcaaattgg tttgaccgat ggtcttactt tcatttctta tccctccaag
     4201 tttttcgatt atttaaagcg gtaagtgata cgtccacgtg tcaagtcata tggactcatt
     4261 tcgacagtaa cacgatctcc cgctaaaata cgaatatagt ttttacgaat tttaccagaa
     4321 actgttgcta aaatctgatg tccattttca agttcaaccg taaacattgc attcggcatt
     4381 gtatcaacta ctttgccttc aacttcaatc acatcgtctt ttgccacgca aaagcacctc
     4441 cataaatttc gattcgatgc ctccagacac agagacaaca attataagtc agactatctc
     4501 agtataacat ttgtagcgga tttttgcaag tgtgaaaaac gctttatttc aaatttgtca
     4561 atactttttc aatatctgag aagacatcat tgatattttg attaccttcg atgtcatgaa
     4621 ccaaaccttt ggcacggtag tgagcaatga ttggttctcc ttgagcaata ttaacatcca
     4681 aacgacgttt tactgtctca ggcttatcat cttcacgttg gtagtaatct tcttctttat
     4741 agtcaactgg tgggttaaag accttgtgga aagtttctcc agttacgcgg tggatgatac
     4801 gcccactcaa acgttccaaa aggctgtcag ggttcacttc aatattgata acaccttcta
     4861 gttcaatgcc aagttcagcc aatgttttgt ccaaggcatg agcttgttca attgtacgtg
     4921 ggtaaccatc caataagaat cctgtttctt taatatcatc ttgtgaaaga cgttctttta
     4981 cgattccatt tgtaacttcg tcaggaacca attcaccctt gtcaatatat gacttagcaa
     5041 gaacacccat ttcagtttga tttgccattg cagcgcggaa catatcacct gttgagatat
     5101 gtgcaacatg gaattgttct acgatttttg ctgcttgagt tcccttacct gcaccaggta
     5161 agcccataat caaaagattc atgatttgat ctccttattt tatttttaaa tagaagcaaa
     5221 aagtcttttc acccctattt aaaaacaaaa tagaagaggg gagatccaac tcttacaaat
     5281 gtcagagttt aaacctccac tctctcagca tttactgatt cagtaaatac ttttattctg
     5341 ttctgtccat aaaaccaaca tacttacgtt tcaataggta accttccaat tgcttgattc
     5401 cttcgatacc tgtagagata atgatcaaga gacttgttcc accaaaggca acaacatcag
     5461 aaagaccaaa tacatcttta gctgcaatcg gtaaaatgga aatcacacca aggaagaggg
     5521 aaccaacagt tgcaagacga cgaagaagtt tagacatata ttcttctgta cctttaccag
     5581 gacgaactcc atggatatag gcaccactct tttgtaggct ctctgctgct ttttcaggat
     5641 taatctgtac aaacgtatag aagaatgtaa agagaataat caacaaagca tacatggcaa
     5701 taccagttgg agaagtagtt gccaacatct cttgtgctac ccttacccaa gcccaatcat
     5761 gacctgtggc actcaaaaac tgaagaatag ccgcaggcgc tgcagtaatc gaactggcaa
     5821 agataacagg gataactcca gcagggttta cctttaacgg aaggtaagag ctagatggag
     5881 caccttgtgc aaccttagta tattggattg gaattttgta ttctgcttgt tgaacataag
     5941 ttgtaaagta aataatcaac aatacagtaa taatcaaaat gattacgaaa atgatagatg
     6001 aagtgatacg gctacttggg acgttcacaa agtagtccac atagatgccc tgaatcatct
     6061 ctggaattga agaaacaatc ccggcaaaga taatcatgga aacaccgttt ccgtatccct
     6121 tatctgtaat ttgctcaccc aaccaagtga caatcatact accagctgtt aagatgatac
     6181 caatcgtcag aaaaacttgt ggagttaaag cagttttaat caattgagct ccagccaagg
     6241 tattaaaacc agctgtaatc ccgatagatt gcacaaaagc gagaactaga gcaatataac
     6301 gagtagcttg attcaatttt cttcgaccta cttccccttg tttaccccac tctacaaact
     6361 tgggtaaaat atccatttgc aagagttgga caacaataga agcggtgata tagggactaa
     6421 ctcctagggc aaaaatcgaa aagtttttta gggcattccc cgacaccaag ctcaacatgt
     6481 ttaagaagga taatccactt aaagcattca agctattggc attcacacca ggaactgtaa
     6541 tgctagttcc gatacgaaag accaaaacga taaaaattgt aaataaaatt tttgatcgaa
     6601 cctgcttgac tttaagagct tctcttaata atttaaaaaa cataggtcac ctctcttaga
     6661 tgacttctac tgaaccacct ttagcagtga tagcttcttc agctgattta gagaatttag
     6721 ctgctttcac agtcaatttc ttagtcaact caccgttacc aagaatttta atacctgact
     6781 tttcagcttt aacaattcct gcttcgataa gaacaactgg agttacttca gcaccatctt
     6841 caaagacgtt caattggtca aggttcacaa ttgcgtattc tttagcgttg atgttagtga
     6901 atccacgttt tggaagacga cggaacaatg gagtttgtcc accttcaaaa ccaaggcgaa
     6961 ctccgccacc gctacgagct ttttgacctt tttgaccacg accagatgtt ttaccgttac
     7021 ctgatgaagt accacgacca acgcggttac gtactttacg agaaccttct gcaggtttca
     7081 attcatgaag tttcattttt attttctcct cttttgtaaa atgctagcgc cgataaggga
     7141 gaaaaggttg tctcccccat caactcgcct atacgatgtc atcatagatg actatatcta
     7201 gttttagggg atggtacagt gcacatcccc taaaacttca ttagtttact tcttcaactg
     7261 ttactaagtg agatactgct gtaatcatac cacggatagc agcgttatct tctttaataa
     7321 cagagctgtt caatttgcca agtccaagtg ctacaacagt tttacgttgt gatggaatgc
     7381 gtccgattgg agacttagtc aaagtaattt taatttgagc cattttatcc cctttcttat
     7441 gccaaatcag aaactgaaat accacgaagg gcagcaattt cttcagcgcg tttcaattgt
     7501 ttcaaacctt caacagttgc acgaacaatg ttgattggag tgttagaacc aagtgattta
     7561 gatgtaatat ctgccacacc tgccaattcc acaacggcac gaactgcacc accagcggca
     7621 actccagaac cttctacagc aggtttcaac aatactttag ctccaccgaa ttctgaaaga
     7681 acttcgtgtg ggattgttgt tccaaccata ggaacttcga tcaagttttt cttagcatca
     7741 tctactgctt tacggattgc ttctggaact tcttgagctt taccagtacc aaatcctacg
     7801 cgaccattgt ggtcaccaac aacaacaaga gctgcgaaac gaagacgacg tccaccttta
     7861 acaacttttg taacacggtt gacagcaact acgcgttctt ctaattcaac tgcattgtct
     7921 ttaaatgcca ttttctagtg tcctcctatt agaatttcaa tccgttttca cgagctgcat
     7981 cagccaaagc tttcacacgt ccgtgatata gatatccacc gcggtcgaac accacttctg
     8041 aaataccttt agcgtttgca cgttctgcaa cgagtttacc gacagcaacg gcttgttcag
     8101 ttttagttcc ttttgaaact tctttatcaa gagttgaagc acttgcgagc gttacacccg
     8161 ctacgtcatc aatcacttga gcgtagatgc ctgtattaga acggaatacg ttcaaacgtg
     8221 ggcgatcagc agttccagag agttttccgc gaacgcgacg gtggcgtttt tggcggagtt
     8281 tgtttttatc tggttttgaa atcacagttt tcacctcttt agttttaaat cgtgtgctat
     8341 gcacaaagtt ggaaaatagg ttggtggttg atgatcaacc actcaacatt atttacctgt
     8401 tttaccttct ttacggcgaa cgaattcacc aacgtaacgg atacctttac ctttatatgg
     8461 ttctggtgaa cgaaggctac gtacgtaagc agctgtttga ccaactactt cttttgaaat
     8521 tccgctaaca acgattgttg ttgggtttgg aagttcaaaa gtaattcctt ctggagcttc
     8581 aacttcgtct ggatgagatt taccaacagc caaaacaagt ttagatcctt gaagctgtgc
     8641 acggtaacca accccacgca tttcaagttc tttcttgaat ccttctgata caccaacaac
     8701 catgttgttc aaaagggcac gagtagttcc gtggatagtt ttcatttctt ttgaatcgtt
     8761 tggacggtga agagttactt cagtaccttc cacacggatt tcaatatctt ttgagaactc
     8821 acgagtaagt tctcctttag gtccttttac agttacaacg ttgtcattgt tagcgagttc
     8881 aacaccagca ggcaacacga taactttatt accaatacgt gacatgttta ttttctcctg
     8941 ttaaattgtc aggccagaac ggccagtttt cacggggttc agatacttat ttagttcaag
     9001 agctgaactg aacacgctct aagaactgtg caaaatagat aagttggctt gtttgtaaac
     9061 aaacttctcc aacttcctaa ttttgctttg ttctttacga gctttgtatc ttgattttac
     9121 caaacgtaag cgataacctc accaccaaca ttcttttggc gtgcttcttt atcagtaagc
     9181 aaaccttcag aagttgaaag gatggcaatt ccaagtccgt taagaacttt tggaaggtct
     9241 tcacgttttt tgtagacacg aagtcctggt ttagaaacac gtttcaagtt agtgataact
     9301 ttctcaccat ttggtccgta tttaagaaat acacggatga cgccttgttt gtcatcttca
     9361 atgatttcaa cgttttttac aaaaccttcg cgtttaagga tttcagcaat cccttttttg
     9421 atgtttgatg caggtacttc aagtacttcg tgtttagctt ggttagcatt acgaatacga
     9481 gttaggaagt ctgcgattgg gtcagtcata accattttgt ttttctcctc ttactagtag
     9541 tttgcaagtt gcacttgcta gttaatacat gatacaaaga gcgtaacagc acgagcaaaa
     9601 atagacaatt taatgcagaa gcaatgctcc aaagcaaatt ggtctttttt gccaaagctg
     9661 tagctcgtgt tcaaattggc aagcccaatt gaacccgggc taaactctgt gtgaaaaaga
     9721 taaactttcc tagaaacttt agtttcttcg tcaagtttcc tatttttact tggagttttg
     9781 acgctcttga tatcttaaat taccaagatg cctttgttac accaggaatt tgacctttat
     9841 gcgcaagttc gcgaaaagcg atacgactca gaccaaattt gcggtaaact gaatgtgggc
     9901 gccccgtaac cctacaacga ttatgtaaac gagtcggtga ggcgttgcga ggtaatttag
     9961 ataaaccttc gtagtcccct gccgccttta atgcagcacg tttttcagca taacggtcaa
    10021 caattttttg gcgtttagcc tctctagcta ccattgattt tttagccatt agatttacct
    10081 cctatattat tttgcaaaag gcattccaag gcctgtaagc aatgcacgtg actcttcgtc
    10141 agtgttagca gttgttacga taacgatgtc aagaccacga gttttgtcaa cgtcatcgaa
    10201 gttgatttct gggaagatta attgttcttt cacaccaagt gtgtagttcc cgcgtccatc
    10261 aaatgatttt gttgggacac cgtggaagtc acgtacacgt ggaagtgaaa ctgataccaa
    10321 tttatccaag aattcgtaca tacgttcacc acgaagggta acttttgcac cgatcgcaac
    10381 accttcacga agacggaagc cggcgattga ttttttagct ttagtgataa gtggtttttg
    10441 acctgagata agtgccaatt cttcagcagc tttttcaagg cttttagcgt ttgatacagc
    10501 ttcaccaaca cccatgttca aaacaatctt atctacttta ggcacagcca tcactgatga
    10561 gtagttgaat tgttctgtca aagcaggaac tacttcatta agatattttt cttttaaacg
    10621 atttgccatt atacttctcc tttccttcgt gattaatcaa gcacttcgcc tgattttttg
    10681 ttgtagcgaa cttttttacc gtctacaaat ttgtaaccaa cacgaccagc tacaccattt
    10741 ttgtccaaaa cttgaacgtt tgatacgtgg atagctgctt ctttctcgat gataccacct
    10801 tgaggaagct cgttagttgg acgttggtgt ttcttaacaa tgttaacacc ttcaacgata
    10861 actttgttta cttttggaag ggcagtaagg acaacagctt ctgttccctt atctttacca
    10921 gcgattacgc gaactttgtc gccttttttt acaaacatta ggtttctcct tgatttttct
    10981 tacgcccata agggcaccct agcttgaagc taggggacta gtttgttcct aaaaattaaa
    11041 gtacttctgg agcaagtgac acgatcttca tgaagccacc ttcacgcaat tcacgtgcaa
    11101 ctgggccaaa gatacgtgtt ccgcgaggag ttttgtcttc acggatgata actgctgcgt
    11161 tttcgtcaaa tttgatgtat gaaccatcag cacgacgagc acctgattta gtacgaacga
    11221 taactgcttt aacaacgtca ccttttttaa ccgcaccacc aggagtagct tgttttacag
    11281 atgccacgat aacatcaccg atgtttgcaa atttacgtcc tgaaccacca agaactttga
    11341 tagtcaagat ttcgcgagca ccgctgttgt ctgcgacttt caaacgagtt tctgtttgaa
    11401 tcatttcagt tttctccttt caggtttgat tagatgatga ccgcttcttc aacaacttct
    11461 acaagacgga aacgttttgt agctgaaagc gggcgagttt ccatgatacg tacgatatcg
    11521 ccttctttgg caacattgtt ttcatcatga gctttgtatt ttttagagta gttaatacgt
    11581 ttaccataga ctgggtggtt acgttttgtt tcaactacaa ctgtgattgt cttgtccatt
    11641 ttgtcagata caacacgtcc aacaagaact ttacgattat tgcgttccat tgaaatttct
    11701 ccttccctag tctattattt cgcttcagat tgaactgttt tgatacgagc gatttgtttt
    11761 ttaacttctt tcaagcgagc tgtttgttcc aattgaccag tagcagcttg gaaacgaagt
    11821 tcaaacaatt cttttttcaa ttcgttttcg cgcttcgcga gttcttcttg agaaagacca
    11881 cgaagttctt taacaaattc ttttacttca ttaagtttca tgccttctcc ttattctgct
    11941 tcacgtttta cgaatttaca tttaactggc aatttgtggc tagcaagtcg aagcgcttca
    12001 cgtgcaatct cttcagatac accagcgatt tcgaacatca ctttaccacg tttaactggt
    12061 gctacccaac cttcaggtgc ccctttacca gatcccatac gcacaccgat agctttagca
    12121 gtgtatgatt tgtgtgggaa gattttaatc caaactttac caccacgttt catgtaacga
    12181 gtcatggcga tacgagcagc ttcgatttgg cggttagtga tccagtggct agttgtagct
    12241 tgaagaccgt attcaccgaa tgctacttct tttccacctt ttgcttcacc gcgcattttt
    12301 ccacggaatt cacgacggtg tttaacacgt ttaggtacta acattggtta tttacctcct
    12361 ttagtgtttt tacgagctgg aagaacttca ccacggtaga tccatacttt aacaccaagt
    12421 ttaccgtatg tagtatctgc ttcttcccaa gcgtaatcga tatctgcacg aagtgtgtga
    12481 agcggaacag ttccttcaga gtatccttca gcacgggcga tatctgcacc gttcaaacga
    12541 cctgatactt gagttttgat tcctttagct ccagcacgca ttgcacgttg gattgcttgt
    12601 ttttgtgcac gacggaaagc aacacgttgc tccaattgac gagcaattcc ttcacctaca
    12661 aggtgagcat ccaaatcagg ttgtttgatt tcgatgatgt tgatgtgtac ttgttttcca
    12721 gtcaatttgt taagttttgc acggagtgca tcaacgttag caccaccttt accgataacc
    12781 atacctggtt tagcagtgtg aagtgaaacg ttaactttgt ttactgcgcg ttcgatttca
    12841 atagttgaaa ctgctgcgtc agcaagttct ttttgaacga atttacggat tgcaagatct
    12901 tcatgaaggt aatccgcgta ttctttttca gcataccatt tggcatccca atcacggatg
    12961 atgccgacac gcataccaat tggatgtact ttttgaccca cgattttacc tccttatttt
    13021 tctgcaacag ctacagtgat gtgagctgta cgtttgttga ttggtgaagc tgaacctttc
    13081 gcacgtggac ggaaacgttt catagttggt ccttcgtttg cgaatgcttc agatactacc
    13141 aagttagctt tatccaaacc aaagttgttt tcagcgttag ctacagctga gttcaaaact
    13201 ttcaagatga tttcagcagc tttgtttgga gtgaatgtca agattgcgat tgcatcggct
    13261 acgcttttac cacggatgtt atcaagaaca agacgtgatt tacgaggtga aacacgtact
    13321 gtacgagcca ttgctttagc tgaagtaatt tctgccattt atgttctcct tattttctac
    13381 gtgttttctt gtcgtctgca gcgtgacctt tgtaagtacg agttggtgca aattcaccaa
    13441 gtttgtggcc taccatgtct tcttggatgt aaacaggtac gtgtttacgt ccgtcataaa
    13501 ctgcaatagt gtaaccaatg aaacttggga agatcgttga acgacgtgac caagttttaa
    13561 taactttttt cttttcgtcg ttagcttgag cttcaacttt tttcatcaaa tgctcatcga
    13621 cgaaaggtcc ttttttaaga ctgcgtccca tttttatatt ttctccttta aatgttgtac
    13681 cacagcggct tgcactccta tggagcgcta ccgagctggc ggatttacta gttgcttaag
    13741 cgactagttt aatattattt ctcgttgcga cgacgaacga taagtttgtc agatttcgct
    13801 ttcttgttac gagttttaag accaagagca ggtttgcccc atggagtaga tggtgcttta
    13861 cgaccaactg gtgctttacc ttcaccacca ccgtgtgggt gatcgttagg gttcattaca
    13921 gaaccacgaa ctgttgggcg gatacctttc caacggctac gtcctgcttt accaaggttt
    13981 acaagtccat gttgttcgtt tccgacaaca ccaactgtag cacggcaagt tccaagaatc
    14041 atacgaactt cacctgattg aagacgaaca agaacgtatt taccttcaga acccaatact
    14101 tgagcagatg caccagcagc acgtaccaat tcaccaccac gacctggttt caactcgatg
    14161 ttgtgaatca aagtaccaac tgggatgtta gcaagtggaa gagcgtttcc gactttgata
    14221 tctgcttctg gacctgaaac gatacgttga cctacttcaa gaccttttgg agcgatgatg
    14281 tatgctttca caccgtcagt gtagtgtaca agagcgatgt ttgcagaacg gtttggatcg
    14341 tactcgattg ttttaacaac tgcttcaacg ttgtctttat tacgtttgaa gtcaaccaaa
    14401 cggtagaaac gtttgtgtcc accaccttgg tgacgaactg tgatacgacc gttgttgtta
    14461 cgaccagcct tgctcttcaa tgcaacaagc aatgattttt caggagtgct tgttgtgatt
    14521 tcagcgaaat ccaaagaagt catattacgg cgaccgtttg ttgttggttt ataaacacga
    14581 attcccacga tatttcctcc ttagattatt cagcttcagc agcaaacaac tcgattgctt
    14641 tagaatcagc tgtaagtgtg atgatagctt ttttagtttt gttagtaaaa ccagtgtaac
    14701 gtccaacacg tttagctttt ggttttacgt tgattgtgtt aacattggca actttaacac
    14761 cttcgaaagc agcttcaaca gcttgcttga tcaaaagttt gtgtgcacga gtgtcaactt
    14821 caaatacata ttttcctgct tcaagttgag ccattgagct ttcagtgatg acaggttttt
    14881 tgataacatc atacaaattc attatgcaag aacctcctcg attttagaga tagctgcttg
    14941 tgtgacaaga agtttgtcgc tatttgcgat gtcaagaaca cttgcagttg tagcagttgc
    15001 aactttcacg tttggaaggt tacgagctga aagagctgcg aattcatttc cttcttcaag
    15061 gataacaaga actttagaat cgatgctcaa tgctgcaaga acttttgcaa attcagcagt
    15121 ttttggagct gtaaatgaaa gagcgtctac agctacgaat ttgttttcag caactttttc
    15181 agagtaaact gatttaagag ctaggcgacg aactttttgt ggaagtttgt agccgtatga
    15241 acgtggagtt ggtccgaaga caacaccacc accacgccat tgtggtgagc ggatagaacc
    15301 ttgacgagca cgtccagttc ctttttgacg ccacggtttg cgtccaccac ctgatactgc
    15361 agagcggttt ttaacagcgt gtgttccttg acgaaggctt gcgcgttggc tgatgattac
    15421 atcaaacaca actgattcat ttggttcgat accaaatact gcatcgttaa gaacaacttg
    15481 gccagcttct ttaccagttt ggtcaaataa tgttacgttt gccattgtga ctgatttccc
    15541 ctttctttat tatttaccag ctttaactgc tgatttgata gtgataagag atttcttagc
    15601 acctggtacg ttacctttga taaggataac gttcttttct ggaacaactt gtacaacttc
    15661 aaggttttga attgttacgc ggtcgccacc catacgccct gcaaggtttt tacctttgaa
    15721 tacgcggtta ggtgcaacag gtcccataga acctggacga cggtggtaac gagaaccgtg
    15781 agccattggt ccacgtgatt gtccgtggcg tttgataaca ccttggaaac ctttaccttt
    15841 agaagtacca gttacgtcaa caacgtctcc agctgcgaat gtttcaactg taatttcagc
    15901 accaacttcc aagccttcaa cgtttttgaa ttcacgaatg aagcgcttag gagccgtgtt
    15961 agctttcgct acatgtcctt tagcaggttt gttgctcaat acttcgcgtt tgtcatcgaa
    16021 accaacttgg atagcgttgt atccgtctgt ttcaacagtt ttaacttgaa gaacaacgtt
    16081 tggagttgct tcaataactg ttacagggat caattcgcca gcttcagtga agatttgagt
    16141 cattcccact tttttcccta agattccttt tgtcatgaga aaatagttcc ttttctatat
    16201 tttttattca aaaagttttt aacgagcgtt ttttatgctc aagtctaaga tacaaagggc
    16261 gtcaaactca acgtgaaaat aggaaactga cacagtgtct agcagacgcg aggaagttta
    16321 tctttttcac accaagttta gcccgtgttc aattagattg aaacaccagc ctctgctctc
    16381 ttatatttta gattaaagtt tgatttctac gtttacacca cttggaagat ccaatttcat
    16441 caaggcatca actgtttttt gagttgggtt aacgatatcg atcaaacgtt tgtgtgtacg
    16501 catttcaaat tgttcgcgag agtctttgta tttgtgagtc gcacgaatga ttgtgtagag
    16561 gctacgttca gttggaagtg ggattggacc cgcaacttgt gcacctgtac gagtagctga
    16621 ttctacgatt tttgcagccg ctgtgtcaag cgtacggtgt tcgtaagctt tcaaacggat
    16681 acggattttt ttgtttgcca tctttttctc cttttcgtct atttaagata ataggctagc
    16741 tccacaagaa aaccgacacg gtgttgcgtg gcaatgcaac cgagcgtgtc gcaacctctt
    16801 gcatcaaaac taaggctgta atttacagca ccataataga ataacacaaa tcccctgcga
    16861 ttgcaaggga tttgttaggt tttttcgttt ttttgggatg aaactgtttt cctacttttt
    16921 ctttctaaaa ttagggattt tcttctatat ttcttctata cataagttaa gtcctcttga
    16981 catcaaattt atcttctgat tgatccacta agatatccgc cttagactca gtttctttat
    17041 agtatcgcag atactgctcc cgtctcatct gatggctagc caatataaag gatgcatcac
    17101 gatttctcac agtcgtatct ctagcaaggc gtcgctttaa ttcggtctcc tcatccgtgt
    17161 agaaacagat ggttttttca aagagttcct tgggtagaaa gccaacagac atcccttcga
    17221 caatcaaaat tggtttggct ccagacaaga cctcactagc cttccaaggt tcttcaattg
    17281 tcaagacatc catactcgcc tgcaaggcaa ggatatctct ctgcaaactc tccagttcat
    17341 gcgccactgg cagaccggct gtcacctttt gattcggcgc gtccttgggt actaccagat
    17401 ggcgtcctga ggtgatatag ggatctgttt ctagcaaatt tactgtagta gaatctaaag
    17461 cttggtacaa ttcctgtgca gaggttgatt tacctgaagc tccatgaccg tatattccca
    17521 gtgtcctgac tttccccgtc tcgatctctg agactagttg atctactaag tcttttttct
    17581 tcattctctc ttcacctgtt catagctttc ttttccgtca ttgagcttgt cccaaatcac
    17641 tacttgccca cttttgagcg atttttgcac atcgataatt cgttggttgg acgaacctcg
    17701 aaactggagc atgagatttc tcttagttcg atcgtatcgt ccatcgacaa gaatgtcaat
    17761 caatgataag agttccagtt tatctggagt ttccaacatc atttcttccc aagtgtagcc
    17821 cgtccaggac caaatgtcct tgtctgacaa ttcctttcgg atgcgtttaa ctagaggcaa
    17881 gagaatgcca gtattgagaa aaggttcccc tcccagcaaa gtcaagcctt gaacataggg
    17941 ttgggcaagg tctgccatga tctgctcttc taattctgct gtatagggaa tgccagcatt
    18001 aaaagaccaa gtcgcaacat tataacatcc ctcgcagtga aacatacagc ctgatacata
    18061 gagagagttg cgcacgcctt cgccgtccac aaagttaaag gccttgtagt caatgatacg
    18121 accttgacta agttcctcgc ttttccattc ttgtggtttt ggattattca ttcgctacct
    18181 ctatccaata acgctcgact ccattgtgag catcctcaaa tattccacca tttgctagaa
    18241 tgactgctct gctagcagga ttattcacgc tacaggtcac cagagctttc ttgatgttct
    18301 tttccttagc aacttgcaag ccctgacgga gagtctcttt tgcataaccc ttgcctcttt
    18361 cagatggacg gatggagtag ccaatgtgcc caccttcttc tagtagaaag ttactgaggc
    18421 gcaaccggag attaagaaat ccaactgctt gacctttctc agaaaaagcc actaactgaa
    18481 ttgcaggaac ccatccttca ggcagattaa tccccatttc ctgttcctga ttgctttcca
    18541 accactcttc atacacaaag ttctctgtat cccaaaatcc tccatcatgg gctgattggc
    18601 tcttttcaaa ctctgtcatc atatctaaaa cagctttctt atccgctaat cttggtctgc
    18661 gtaattccat ccttacctcc cactatgaga aaagcgagag ctggctctca cttttatttt
    18721 ttcttgttct tgtttaaaaa ttgttctctg aagctaagca agcgttcttt cttaccagct
    18781 ccacctttag agtatttctc gtgataacgg gtcacttgtg cgcgcccctt atcgtctagt
    18841 tgatatttcc ccatttcatt tctttctaat ttgttacttg atgcccagct attttaatcg
    18901 ttgaaccatt catatgtttg acacgcgcag cgatttcctt gtgacgcccg ttgaccatcg
    18961 gtcttgcttg aggattgcca agatagccac aagtccgttt aacaacgtct actgttttag
    19021 gatcgctatt gccacagttt ggacaagcaa accctctctc agttggttca aaatcccctt
    19081 caaagtcaca cttgtagcaa cggtcaatcg gagtattggt gcctagatag cctacacggt
    19141 cataagcata atcccagaca gcttccaagg cctttggatt ttgctgaagg actggatact
    19201 cacaataatg gatgaaacca cctgacgcac ctgcttctgg atagactttc tcaaagtcca
    19261 atttttcaaa cggtgttgga tttttacgaa catcgtagtg gaaagagttg gtgtagtatt
    19321 ccttgtctgt gatatcagga atagagccaa acttgtctat atctagtcgg cagaaacggt
    19381 ctgtcagact ttcggatggt gttgagtaga tagagaaatg gtagccatat tggtctgacc
    19441 actcttctac acggcgtttc atatcgtgaa tgatgtctag cgtgaattcc ttagcatctg
    19501 gattactttc ccagctgtta ccaaagaaaa ctgtcgctac ttcatacaag ccgatatagc
    19561 ccagcgaaac ggtcgcacga cgattcttaa agagctggtc aacactttct tctttaccta
    19621 gacgatggcc aaaagcaccg tactgataaa gaataggagc atttgctggt gtcgcctctt
    19681 tagtgcgttc gacacggtaa acaagagcat cttctgcgat attcattcgc tcgttgaaga
    19741 tttcccagaa cttattcata tcaccttcag actcaagagc aatacgaggc agattaaccg
    19801 tcacaacacc cagattcatg cgacctgaat tgacttctac accattttca tccttccatc
    19861 cttggaggaa agaacggcaa cccataggca ccttgaagga tcctgtcaag tcaacaatct
    19921 tatcgtaaga taaaacatct ggatacatcc gtttggttgc acactcaaga gccaactgct
    19981 tgatgtcgta gttgggagtt ccttcctcta aattaaggcc tcttttcaga gtgaaaataa
    20041 gtttaggaaa gatagccgta cggtgttctg aaccaagacc cttgattcga atgtttaaaa
    20101 tagctttttg aatttctcgt tcaaaacgac tggttcccag accaaaacct aacgaagtaa
    20161 aaggtgtttg tccatttgaa gtgaagagag tattgatttc atactcaaga gattgcatgg
    20221 catcgtagat gtccttttgc gttttcttcc aagcgtaatc ttcccgtttt tcaggcaata
    20281 cccactcttc tgcatctttg agatgttttt gatagttctt ctctgcataa ggcgccaaaa
    20341 tttcatcgat acggtcagct gaacagccac cgtactggct agaagcaacg ttggcaatga
    20401 tttgagaaat ctgtgctgtc gcagtctgga tagacttggg actctctacc tctgcatttc
    20461 caatcttaaa accattttcc aacataccct taaaatcaac caaacagcag ttggtcatag
    20521 gggtataggg actgtagtcc aaatcgtgat agtggatatc ccccttttgg tgggcattgg
    20581 ctacgtgctt aggaagcatt tgcagtccga ttgatttccc aacaatccct gctgtcaaat
    20641 cacgctgagt gttaaagaca tcactgtctt tattagcatt ttcattgaca actgtctggt
    20701 ctttgttgag aagtttatga atactaaagt tgatatccgt cgcttttgag cgctcaaaat
    20761 ccctctgtgt ccgataagta atatactcct cagccagcgc atattctttg gcttcaagga
    20821 gttcatgttc tacgatattt tgaatttcgt aaatcttaat tccctgtgga aagcgactat
    20881 gaatttctgt aataattcgc tcagtcagat cattgaggcg tttttcaacc aggggtgtca
    20941 catccataac cttgtcagcc gccttgtgga gagccttgtc aatcttgtcc acatcaaata
    21001 caacacgtct cccatctcgt ttctcgacga agagagttgg caaaattgta attttttctt
    21061 ctagtgcaat catacgcatg ctcttttcta aaatgttctt tctaaaaagt attataccac
    21121 tctaaaaa
//
LOCUS       DAVKGC010000025        21040 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKGC010000025 DAVKGC010000000
VERSION     DAVKGC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.025"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            97..531
                     /locus_tag="VW049_000768"
     CDS             97..531
                     /locus_tag="VW049_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9431930.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            543..1733
                     /locus_tag="VW049_000769"
     CDS             543..1733
                     /locus_tag="VW049_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU9431931.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGETREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1744..2235
                     /locus_tag="VW049_000770"
     CDS             1744..2235
                     /locus_tag="VW049_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9431932.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2250..3029
                     /locus_tag="VW049_000771"
     CDS             2250..3029
                     /locus_tag="VW049_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9431933.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            3016..3834
                     /locus_tag="VW049_000772"
     CDS             3016..3834
                     /locus_tag="VW049_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9431934.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3834..4127
                     /locus_tag="VW049_000773"
     CDS             3834..4127
                     /locus_tag="VW049_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9431935.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4149..6050
                     /locus_tag="VW049_000774"
     CDS             4149..6050
                     /locus_tag="VW049_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9431936.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMESCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFADKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPRSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSKDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6110..7111
                     /locus_tag="VW049_000775"
     CDS             6110..7111
                     /locus_tag="VW049_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9431937.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7221..7556)
                     /locus_tag="VW049_000776"
     CDS             complement(7221..7556)
                     /locus_tag="VW049_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431938.1"
                     /translation="MKSLARLLIIHVFISIFLFFALTSGAISHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7567..7809)
                     /locus_tag="VW049_000777"
     CDS             complement(7567..7809)
                     /locus_tag="VW049_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431939.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7820..8362)
                     /locus_tag="VW049_000778"
     CDS             complement(7820..8362)
                     /locus_tag="VW049_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU9431940.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIVLFYLM
                     IFGNIIPLLLAYKYPQEVALIYPPLILVIALIASGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(8378..8572)
                     /locus_tag="VW049_000779"
     CDS             complement(8378..8572)
                     /locus_tag="VW049_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9431941.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            8738..9688
                     /gene="rsmH"
                     /locus_tag="VW049_000780"
     CDS             8738..9688
                     /gene="rsmH"
                     /locus_tag="VW049_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9431942.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            9700..10017
                     /gene="ftsL"
                     /locus_tag="VW049_000781"
     CDS             9700..10017
                     /gene="ftsL"
                     /locus_tag="VW049_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9431943.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            10021..12273
                     /gene="pbpX"
                     /locus_tag="VW049_000782"
     CDS             10021..12273
                     /gene="pbpX"
                     /locus_tag="VW049_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU9431944.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            12275..13255
                     /locus_tag="VW049_000783"
     CDS             12275..13255
                     /locus_tag="VW049_000783"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9431945.1"
                     /translation="MFISISAGIVTFLLTLVEIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            13864..15969
                     /locus_tag="VW049_000784"
     CDS             13864..15969
                     /locus_tag="VW049_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9431946.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLAAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(16265..16747)
                     /locus_tag="VW049_000785"
     CDS             complement(16265..16747)
                     /locus_tag="VW049_000785"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9431947.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(16842..18326)
                     /locus_tag="VW049_000786"
     CDS             complement(16842..18326)
                     /locus_tag="VW049_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9431948.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            18479..20086
                     /locus_tag="VW049_000787"
     CDS             18479..20086
                     /locus_tag="VW049_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9431949.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTAAKEVLEKQVLAPWDAFCVELL"
     gene            20245..20418
                     /locus_tag="VW049_000788"
     CDS             20245..20418
                     /locus_tag="VW049_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU9431950.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNIVN"
     gene            complement(20411..21040)
                     /locus_tag="VW049_000789"
                     /pseudo
     CDS             complement(20411..21040)
                     /locus_tag="VW049_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         6357 a         3782 c         4761 g         6140 t
ORIGIN      
        1 tattttttta aaaaaaacta ttgacaagat aaaaataaag gattacaatg gggaatataa
       61 agtaaaccgg taaacctaaa caagaaagga gaaaagatga aaattgtact tgtagggcat
      121 ggacattttg ctacagggat ttatagttct ttacaattga ttgcaggcaa tcaagaaaat
      181 gtggaggcga ttgactttgt ggaaggaatg tcagcagatg aactcaagca aaaaatctta
      241 cttgcaattt caaatgaaga agaagtttta atcctaagtg atctcttggg aggatcgcca
      301 ttcaaggttt cttctaccat aatgggagaa aatccagcca aaacaatgaa tgttctctcg
      361 ggtttgaact tagccatgtt aatggaagca gtctttgcta gaatggctca tagctttgat
      421 gaggttgtta ataaatcagt agtggcggcc cagggcggag tcgtaaatgg taaagaattg
      481 ttttcaacgg atgcagagga agaggaagaa gatttcgaat cgggtattta aagggtgaaa
      541 gaatgataaa aaaggttacg attgaaaaaa taaagtcgcc tgagcgcttc ttagaagtac
      601 cacttctgac gaaagaagaa gtcggccagg caatcgataa ggttattcgg cagttagaac
      661 tcaaccttga ctatttcaag gaagatttcc cgacgccagc tacctttgat aatgtctatc
      721 caatcatgga taacacggaa tggaccaatg gtttctggac aggagaactg tggttggctt
      781 atgaatacag tcaacaggat gcatttaaaa acatcgctca taaaaatgtt ctttctttcc
      841 tggatcgtgt caataagaga gtagaattgg atcatcatga tctcggcttc ttgtacacac
      901 cgtcttgtat ggctgaatat aagataaatg gagatggaga gactagagaa gcaaccttga
      961 aagctgcaga taagttgatt gaacgctatc aagaaaaagg tggttttatt caagcttggg
     1021 gagacttggg caagaaagag cattaccgtt tgattatcga ctgcttgctc aatatccaac
     1081 tcttattctt tgcttatcaa gaaacaggcg atcaaaaata ctacgatatt gcagaaagcc
     1141 atttctatgc ttcagctaat aatgtaatcc gtgatgacgc ttcgtccttc cacaccttct
     1201 attttgatcc tgagacaggt caacccttta aaggtgtaac gagacaaggg tatagtgatg
     1261 attcatgctg ggcacgtggt caatcatggg gagtctatgg tattcctttg acttatcgtc
     1321 acttaaaaga cgagtcctgc tttgacttgt ttaagggtgt gaccaattat ttcttgaatc
     1381 gtctgccaaa agatcatgtg tcctattggg atttgatttt taatgatggt agtgatcaat
     1441 cacgagattc ttcagcaaca gctatcgccg tctgtgggat tcatgaaatg ctaaaacatc
     1501 tcccagaggt ggatgctgac aaagatattt ataaacatgc tatgcatgcc atgcttcgtt
     1561 ccttgatcga acattatgca aatgatcaat ttacccctgg tgggacaagt ctcctccacg
     1621 gtgtgtactc atggcattca ggtaaaggag tggatgaagg caatatctgg ggtgactact
     1681 attacctaga agcccttatc cgtttctaca aagactggaa cctatattgg taggaggaga
     1741 aatatgacaa tgccaaatat tattatgacc cgtatcgatg aacggttgat tcatggacaa
     1801 ggacaacttt gggtaaaata cctaggttgt aatacggtca ttgttgccaa tgacgaagta
     1861 agcacggaca agatgcaaca aactctgatg aaaacagttg tgccagactc agttgccatg
     1921 cgtttcttcc ctttgcaaaa ggtgattgat atcattcaca aggctaatcc tgctcaaacg
     1981 atctttatcg ttgtaaagga tgtaaaggac gctttaacct tggtagaagg tggtgtccct
     2041 atcaaagaaa tcaatattgg gaacattcac aatgcccctg gtaaagagca agtgacacgc
     2101 tccatcttcc tgggtgaaga ggacaaggca gccctcaagg aattaagcca aactcatcaa
     2161 gtaacattta atacgaaaac aactccaaca ggaaatgatg gagctgttca agtcaacatt
     2221 atggactata tttaaaagag gagatcgtta tgtcgattaa tgtatttcaa gcgattttaa
     2281 ttggattatg gacagctttc tgttttagtg gaatgctgtt aggaatttac accaatagat
     2341 gtattgttct gtcatttggt gtcggaatta ttctaggtga tctgcctact gctcttgcaa
     2401 tgggagctat tggtgaattg gcttatatgg gattcggtgt tggtgctgga ggtactgttc
     2461 caccaaaccc aatcggacct ggtatctttg gtaccttgat ggctatcact agtgctggta
     2521 aagtcagtcc agaagcggct cttgccctct ctactccgat tgctgtggcg attcaattct
     2581 tacaaacttt cgcctacact gtacgtgctg gtgcgcctga aacggcgatg aagcacttga
     2641 aaaaccataa tttgaagaaa ttcaagttca ctctaaatgc aacaatttgg ttgtttgcct
     2701 ttattggatt taccttgggt tgcttgggtg ccctttcaat ggataccttg ttgaaactcg
     2761 tagactacat tccaccggta ttacttacag gtttgacagt tgctggtaaa atgctcccag
     2821 ctatcggttt tgcgatgatc ttgtcagtga tggctaagaa agagttgatt ccctttgtct
     2881 tgttgggata tgtttgtgca gcttatctaa acatcccaac aattggtatt gcaattgtag
     2941 gtactatctt tgctttgatt gaattttata acaagccaaa aacagcggat catgtggtag
     3001 aggaggaagc acacgatgac tggatctaac aaattaacaa aacgtgatta tcttaaaacg
     3061 tctttgcggg cattcttttg tcaaaatgga tttaactata gtaactatca agggttggga
     3121 tatgccaatg tgatgtatcc tgctttgaaa aaacactatg gagaggatca ggaaggtttc
     3181 taccaagcct tggaagaaaa ctgtgaattc tataatacca acccacactt cctgcctttt
     3241 attaccagct tgcatcttgt aatgttggaa aatggtcgac caacaaaaga aacacgtagc
     3301 atcaagatgg ccttgatggg accattggca ggtattgggg attctctttc tcaattctgt
     3361 ttagctccat tgttctcaac catcgcagct tcgtttgctc aagaaggctt ggttgtcggt
     3421 ccaatcttgt tcttccttgc gatgaatacg attttaacag cgattaaatt gtcaactggt
     3481 ctgtatggat acaaactagg aacaactgtg attgataaac taagcgagca gatggcaacg
     3541 atttctcgta ttgccaatat tatcggtgta accgtaattg ctggtttggc agcgacatct
     3601 gttaagatta tggtgccgat tacctttgct gcaggggaag ttaaagcaga cgctaaacaa
     3661 agtatcgtaa gtattcaggg aatgcttgat aaggttgctc cagctcttct accagcccta
     3721 tttacacttt tagtttacta cttgatcaaa gaaaagaaat ggacaacata taaactcgtt
     3781 attttaacag ttatcatcgg aattatcgga agctggctta agattatagc ttaatggata
     3841 caacattgtt ttatggaata gtgattgtct tggcagtgag tcccctttta ctgtcaagct
     3901 ttcattctat tcgtcagcaa aagttgcttc gcaaacagat ggagcaacga caagagtatt
     3961 tagcttcttt aacatctggt gatgaagtgt tgttattgtc tggaattcat ggaaaaatca
     4021 tttctatcaa agatgacttg gtctccttgc agattgcaaa aggtgtggtc atctatgtag
     4081 aaaaggaaag tgtaatggga aagacaaaag aactgctttt taagtagttc tttttctggt
     4141 aatagataat gaaagaagaa agaagacaat tttttgaaag agtcgatgga aaccaatgtc
     4201 gtgattatat cttgtcccac tgttcaaaag actatgagaa agtcaagagt tcccttgaac
     4261 gcttgatgga caatcgtttt atgtttgata gtccttggga tatggagtct tgttcaaaaa
     4321 tccatcaaat ccagccgatg gtatgggatc aagtatttga agatgatcca gaatggtctt
     4381 atatgctcaa tcgacaagaa tatctcttgc agtttatgat agggtatctg gtagaaggag
     4441 ataaggacta tattcaaaag tgcaagttct ttctatttga ttggattgag caggtgagag
     4501 aattttctcc tcaatccttg atgactagaa ccttggatac gggtattcgt tcctttactt
     4561 ggttgaaact actcttgctc ctcttgaaat ttgacttgct agaggagaaa gaactagaga
     4621 aaattttggt cagtctagaa aagcagattg actttatgaa aagccactat cgcgccaagt
     4681 acacccttag taactgggga attttacaaa caattccgat gcttgctatc tatcatttct
     4741 ttgcagataa gatggaccta gaagaagctt accattttgc ttcagaggag ttgaaacagc
     4801 aaattgagac acagatttta ggagatggaa gccagtttga acagtcgatt ctctatcatg
     4861 tagaggttta taaagccttg ctggatttgt gtctcttgct tccagacttg caagatagtt
     4921 accaagagtt gctggaaaag atggcgacct atattcaaat gatgacaggc ttagatggac
     4981 gaactttggc ttttggtgat agcgattcta cagaaacgac agaaatgttg agcctgtctg
     5041 ctgtggtttt gaacaaggaa gaccttctta acggtctgga tgttaaagtc gatttgctta
     5101 gcctcttgtt cctggggcga gaaaaggtca agcgactgca ggaatttgaa aagagagctt
     5161 ggcagcctag gtccatgatc tttgaagact ctggacatgt ctgcattaag gatgaacatc
     5221 gttatctatt tttcaaaaat ggtccgctag gaagtgccca tagccatagt gacgagaata
     5281 gtttttgctt acagtatcaa ggccaaccta ttttcataga tgctgggcgt tattcttatc
     5341 gggagatata tgaacgttat ctcttaaaga gtgcttggag tcattcgacc tgcattgtag
     5401 atgggaaagc tccggaaaga atcacgggat cctgggaata tgaatactat cctcactccc
     5461 tgttttgtca ccataaagaa agggagggag tgcattatat tgaaggggct tattggtcag
     5521 cagaacctga tttgccttat cttcacaaga gaaaaatcct catgttggta gaggatgtct
     5581 ggctcttggt agatgacatc aggtgtcaag gtcagcatga ggcgttgact cagtttatcc
     5641 ttgacaagga tgtgacctat caagatggga aaatcaatca gttgagacta tggagtgaag
     5701 ttgattttga tttggaagat accatcattt ctcctaaata caatgagctt gaaagaagta
     5761 gcaaactcac caagcgccaa ttctttgaga atcagatgct ggattatacc atcattgcgc
     5821 atgagagttt tgaaatcatc cgtcattctg tctaccagac agatgatcgt gaagtggaaa
     5881 atgctctggc ttttgaagtg aaaaatgacg aaacagacaa gctgattctg ttattaagca
     5941 aggatatttg tgtaggtgaa aaattgtgcc tcgttgacgg aacaaaaatg cgtggaaaat
     6001 gtctagtata tgataaaata aatgagagaa tgattcgctt gcagtgctag aaataggcat
     6061 tttgaatagt gaatatgtta taataagtat tagtaggagg tgttttagat tggagaagaa
     6121 actgaccata aaagacattg cggaaatggc tcagacctcg aaaacaaccg tgtcatttta
     6181 cctaaacggg aaatatgaaa aaatgtccca agagacacgt gaaaagattg aaaaagttat
     6241 tcatgaaaca aattacaaac cgagcattgt tgcgcgtagc ttaaactcca aacgaacaaa
     6301 attaatcggt gttttgatcg gtgatattac caacagtttc tcaaaccaaa ttgttaaggg
     6361 aattgaggat atcgccagcc agaatggcta ccaggtaatg ataggaaata gtaattacag
     6421 ccaagagagt gaggaccggt atattgaaag catgcttctc ttgggagtag acggctttat
     6481 tattcagccg acctctaatt tccgaaaata ttctcgtatc atcgatgaga aaaagaagaa
     6541 aatggtcttt tttgatagtc agctctatga acaccggact agctgggtta aaaccaataa
     6601 ctatgatgcc gtttatgaca tgacccagtc ctgtatcgaa aaaggttatg agcattttct
     6661 cttgattaca gcggatacga gtcgtttgag tactcggatt gagcgggcaa gtggttttgt
     6721 ggatgcttta acagatgcta atatgcgcca cgccagtcta accattgaag ataagcatac
     6781 gaatttggaa caaattaagg aatttttaca aaaagaaatc gatcccgatg aaaaaactct
     6841 ggtatttatc cctaactgtt gggccctacc tctagtcttt accgttatca aagagttgaa
     6901 ttataacttg ccacaagttg ggttgattgg ttttgacaat acggagtgga cttgcttttc
     6961 ttctccaagt gtttcgacgc tggttcagcc ctcctttgag gaaggacaac aggctacaaa
     7021 gattttgatt gaccagattg aaggtcgcaa tcaagaagaa aggcaacaag tcttggattg
     7081 tagtgtgaat tggaaagagt cgactttcta aaatgaagga aaatgacttg caatctctgt
     7141 taagaaataa aataatccca cctagaacaa gctaggtggg attatttgcc tataaaatga
     7201 gaaattatgg gagcaagctc ctaaatcaac tgtttttgat ctacttcttt aactacttga
     7261 taaaagttat agaagtaggc caaacttgaa atgatggtta cgactaggaa tattgaaaat
     7321 ttccattgga cagggttggt taaaagttgt ggaaaggata tgaggagaaa gaagagggct
     7381 gcgttgagga caggtatccg ttttgattgt attttctcaa gtcctttatt gagcgcagga
     7441 agaaagagga gtaggagtag taaaactgta tgagaaatag ctcctgaagt aagggcgaag
     7501 aaaaggaaaa tactgataaa aacatgaatg atcagtagtc tagctagtga tttcataagg
     7561 cacctcctaa tcctggtctt ttttagctct tgcaatacga agtgagtcga caatatgtat
     7621 catcactccg aaaaagaaag ctcccagtat agttttaaaa atatgttttg tatttagaag
     7681 agaactgata aaatttggat tttcacttgt tagggtatca atgagtggaa ttataaaaaa
     7741 tatcactgtt ccataaatcg aacctgcttt cagaccagga taacgtaact gtttcttttc
     7801 ttttttcatg agtttcctcc taatcctcat cttgattttt cttagttttt gcaatgcgac
     7861 gggagatgag gaactgtatg ctcgctccga agaaaataga accgagaata cttgatacac
     7921 catttcttat agtgagaaga gaatgaaaat agtcctgacc ttcatctatg agtatactga
     7981 gaagaggagt tataaaaaac atccatagac caaagaacaa acctgctttc agaccagggt
     8041 agtgtagttg cttgctttct ttctcattca gcatatctgg ttcaatgact gtgatgcctg
     8101 tttttttcat ttggtaggtg acatagccag aagcgatgag ggcaatcact aaaatcagag
     8161 gaggatagat tagagccact tcttgagggt atttataggc cagaaggagt ggaataatat
     8221 ttccgaaaat catcagataa aagaggacga taaagacttg gttcccaata cgatcggcct
     8281 cacgccgttt gtattcgtca aggggaccag aaataccgta tgtgcgtttg atcagttttt
     8341 cagtgaaggt ttcttttttc atgagtttgc tcctttttta aaaatcttcc tcccaaaaga
     8401 gactgttgag gtcagtttgg aggctgcggg cgagattgag acagagttcc aaggttggat
     8461 tgtacttgtc gttttcaatc atattgatag tctgtctcga gacaccgata tccttggcga
     8521 gttcgagctg ggaaataccc aattccttgc gaaattcttt cacacgattc atctggtctc
     8581 ctttctgatt tatgtcgtat atatttgact atattatagt cttttaaaca taaagtgtca
     8641 agtatttttg acatattttt tgaagaaata gtagtctcct tgtcctattt gtctgacaag
     8701 tgcaagctgg tcggatttgt ggtaaaatag ataagatatg acaaaagaat ttcatcatgt
     8761 aacggtctta ctccacgaaa cgattgatat gcttgacgta aaacctgacg gtatctacgt
     8821 tgatgcgact ttgggtggag caggccatag cgagtattta ttaagtaaat taagtgaaaa
     8881 aggccatctc tatgcctttg accaggatca gaatgccatt gacaatgcgc aaaaacgctt
     8941 ggcaccttac attgagaagg gaatggtgac ctttatcaag gacaacttcc gtcatttaca
     9001 ggcacgtttg cgcgaagctg gtgttcagga aattgatgga atttgttatg acttgggagt
     9061 gtctagtcct caattagacc agcgtgagcg tggtttttct tataaaaagg atgcgccact
     9121 ggacatgcgg atgaatcagg atgctagtct gacagcgtat gaagtggtga acaattatga
     9181 ctatcatgac ttggttcgta ttttcttcaa gtatggagag gacaaattct ctaaacagat
     9241 tgcgcgtaag attgagcaag cgcgtgaagt gaagccgatt gagacaacga ctgagttagc
     9301 agagattatc aagttggtca aacctgccaa ggaactcaag aagaaggggc atcctgctaa
     9361 gcagattttc caggctattc gaattgaagt caatgatgaa ctgggggcgg cagatgagtc
     9421 catccagcag gctatggata tgttggctct ggatggtaga atttcagtga ttacctttca
     9481 ttccttagaa gaccgcttga ccaagcaatt gttcaaggaa gcttcaacag ttgaagttcc
     9541 aaaaggcttg cctttcatcc cagatgatct caagcccaag atggaattgg tgtcccgtaa
     9601 gccaatcttg ccaagtgcgg aagagttaga agccaataac cgctcgcact cagccaagtt
     9661 gcgcgtggcc agaaaaattc acaagtaaga gggaaaaaga tggcagaaaa aatggaaaaa
     9721 acaggtcaaa tactacagat gcaacttaaa cggttttcgc gtgtggaaaa agctttttac
     9781 ttttccattg ctgtaaccac tcttattgta gccattagta ttatttttat gcagaccaag
     9841 ctcttgcaag tgcagaatga tttgacaaaa atcaatgcgc agatagagga aaagaagacc
     9901 gaattggacg atgccaagca agaggtcaat gaactattac gtgcagaacg tttgaaagaa
     9961 attgccaatt cacacgattt gcaattaaac aatgaaaata ttagaatagc ggagtaagat
    10021 atgaagtgga caaaaagagt aatccgttat gcgaccaaaa atcggaaatc gccggctgaa
    10081 aacagacgca gagttggaaa aagtctgagt ttattatctg tctttgtttt tgccattttt
    10141 ttagtcaatt ttgcggtcat tattgggaca ggcactcgct ttggaacaga tttagcgaag
    10201 gaagctaaga aggttcatca aaccacccgt acagttcctg ccaaacgtgg gactatttat
    10261 gaccgaaatg gagtcccgat tgctgaggat gcaacctcct ataatgtcta tgcggtcatt
    10321 gatgagaact ataagtcagc aacgggtaag attctttacg tagaaaaaac acaatttaac
    10381 aaggttgcag aggtctttca taagtatctg gacatggaag aatcctatgt aagagagcaa
    10441 ctctcgcaac ctaatctcaa gcaagtttcc tttggagcaa agggaaatgg gattacctat
    10501 gccaatatga tgtctatcaa aaaagaattg gaagctgcag aggtcaaggg gattgatttt
    10561 acaaccagtc ccaatcgtag ttacccaaac ggacaatttg cttctagttt tatcggccta
    10621 gctcagctcc atgaaaatga agatggaagc aagagcttgc tgggaacctc tggaatggag
    10681 agttccttga acagtattct tgcagggaca gacggcatta ttacctatga aaaggatcgt
    10741 ctgggtaata ttgtacccgg aacagaacaa gtttcccaac gaacgatgga cggtaaggat
    10801 gtttatacaa ccatttccag ccccctccag tcctttatgg aaacccagat ggatgctttt
    10861 caagagaagg taaaaggaaa gtacatgaca gcgactttgg tcagtgctaa aacaggggaa
    10921 attctggcaa caacgcaacg accgaccttt gatgcagata caaaagaagg cattacagag
    10981 gactttgttt ggcgtgatat cctttaccaa agtaactatg agccaggttc cactatgaaa
    11041 gtgatgatgt tggctgctgc tattgataat aatacctttc caggaggaga agtctttaat
    11101 agtagtgagt taaaaattgc agatgccacg attcgagatt gggacgttaa tgaaggattg
    11161 actggtggca gaatgatgac tttttctcaa ggttttgcac actcaagtaa cgttgggatg
    11221 actctccttg agcaaaagat gggagatgct acctggcttg attatcttaa tcgttttaaa
    11281 tttggagttc cgacccgttt cggtttgacg gatgagtatg ctggtcagct tcctgcggat
    11341 aatattgtca acattgcgca aagctcattt ggacaaggga tttcagtgac ccagacgcaa
    11401 atgattcgtg cctttacagc tattgctaat gacggtgtca tgctggagcc taaatttatt
    11461 agtgccattt atgatccaaa tgatcaaact gctcggaaat ctcaaaaaga aattgtggga
    11521 aatcctgttt ctaaagatgc agctagtcta actcggacta acatggtttt ggtagggacg
    11581 gatccggttt atggaaccat gtataaccac agcacaggca agccaactgt aactgttcct
    11641 gggcaaaatg tagccctcaa gtctggtacg gctcagattg ctgacgagaa aaatggtggt
    11701 tatctagtcg ggttaaccga ctatattttc tcggctgtat cgatgagtcc ggctgaaaat
    11761 cctgatttta tcttgtatgt gacggtccaa caacctgaac attattcagg tattcagttg
    11821 ggagaatttg ccaatcctat cttggagcgg gcttcagcta tgaaagactc tctcaatctt
    11881 caaacaacag ctaaggcttt agagcaagta agtcaacaaa gtccttatcc tatgcctagt
    11941 gtcaaggata tttcacctgg tgatttagca gaagaattgc gtcgcaatct tgtacaaccc
    12001 atcgttgtgg gaacaggaac gaagattaaa aacagttctg ctgaagaagg gaagaatctt
    12061 gccccgaacc agcaagtcct tatcttatct gataaagcag aggaggttcc agatatgtat
    12121 ggttggacaa aggagactgc tgagaccctt gctaagtggc tcaatataga acttgaattt
    12181 caaggttcgg gctctactgt gcagaagcaa gatgttcgtg ctaacacagc tatcaaggac
    12241 attaaaaaaa ttacattaac tttaggagac taatatgttt atttccatca gtgctggaat
    12301 tgtgacattt ttactaactt tagtagaaat tccggccttt atccaatttt atagaaaggc
    12361 gcaaattaca ggccagcaga tgcatgagga tgtcaaacag catcaggcaa aagctgggac
    12421 tcctacaatg ggaggtttgg ttttcttgat tacttctgtt ttggttgctt tctttttcgc
    12481 cctatttagt agccaattca gcaataatgt gggaatgatt ttgttcatct tggtcttgta
    12541 tggcttggtc ggatttttag atgactttct caaggtcttt cgtaaaatca atgaggggct
    12601 taatcctaag caaaaattag ctcttcagct tctaggtgga gttatcttct atcttttcta
    12661 tgagcgcggt ggcgatatcc tgtctgtctt tggttatcca gttcatttgg gatttttcta
    12721 tattttcttc gctcttttct ggctagtcgg tttttcaaac gcagtaaact tgacagacgg
    12781 tgttgacggt ttagctagta tttccgttgt gattagtttg tctgcctatg gagttattgc
    12841 ctatgtgcaa ggtcagatgg atattcttct agtgattctt gccatgattg gtggtttgct
    12901 cggtttcttc atctttaacc ataagcctgc caaggtcttt atgggtgatg tgggaagttt
    12961 ggccctaggt gggatgctgg cagctatctc tatggctctc caccaagaat ggactctctt
    13021 gattatcgga attgtatatg tttttgaaac aacttctgtt atgatgcaag tcagttattt
    13081 caaactgaca ggtggtaaac gtattttccg tatgacgcct gtacatcacc attttgagct
    13141 tgggggattg tctggtaaag gaaatccttg gagcgagtgg aaggttgact tcttcttttg
    13201 gggagtggga cttctagcaa gtctcctgac cctagcaatt ttgtatttaa tgtaagaatg
    13261 gcaccctgat gtttcagggt gtttttgtat ttaaatacac aatgaaaatc aaagaacaaa
    13321 ctagaaagct aactttaggc tgctcaaaat atagtagatt gaaactagaa tagtacacat
    13381 ctgcttctaa aatattgtta gaaatcgatt tgactgtcct gatcgatttg tcctgttctt
    13441 atttcatttt aatatatagt tttgagatgg ttgatagaac tgacgaagtc agctcaaaac
    13501 atggttttga ggttgtagat aaggagaagc tgatgtggtt tgaagaggtt ttcgaagagt
    13561 gtaaaaaaat attggtcagt taaagtcaaa actcttgacc ttttctgacc aatgtagtat
    13621 attatgaacg taaggtaaat gaaagcctta ctagaacact tatttaaagt aaaatagaaa
    13681 gaggtgggtc tatgtttcat ctagaaattt tcagaagtaa agatagtcta ctcctgcttg
    13741 aaaaagaaaa accagaaata gtacgtaggg tagcggttta gctagtctaa attttttaaa
    13801 acaatggtca aagatagtca atatcagtag tcataactaa gtaaacaaaa agaggtaaag
    13861 aatatgaata acaactttaa taattttaat aacatggatg atttatttaa ccaattgatg
    13921 ggtggtatgc gaggatatag ttctgaaaat cgccgttact taattaatgg acgcgaagtc
    13981 acacctgagg aatttgctca ctatcgtgcg actggtcaat taccaggaaa tgcagaaact
    14041 gatgtgcaaa tgccacaaca ggcatcaggt atgaaacaag acggtgtcct tgcaaaacta
    14101 ggtcgaaact tggcagcaga agcgcgtgag ggcaagttgg atcctgttat cggacgaaac
    14161 aaggaaattc aagaaacatc tgaaatcctc tcacgtcgca ccaagaacaa tcctgttttg
    14221 gtcggagatg caggtgttgg taagacagca gttgtcgaag gtctagcgca agccattgtg
    14281 aacggagatg ttcctgctgc tatcaagaac aaggaaatta tttctattga tatctcaggt
    14341 cttgaggctg gtactcaata ccgtggtagc tttgaagaaa atgtccaaaa cttagtcaat
    14401 gaagtgaaag aagcagggaa tattatcctc ttctttgatg aaattcacca aattcttggt
    14461 gctggtagca ctggtggaga cagtggttct aaaggacttg cggatattct caagccagct
    14521 ctctctcgtg gagaattgac agtgattggg gcaacaactc aagacgaata ccgtaacacc
    14581 atcttgaaga atgctgctct tgctcgtcgt ttcaacgaag tgaaggtcaa tgctccttca
    14641 gcagagaata cttttaaaat tcttcaaggc attcgtgacc tctatcaaca acaccacaat
    14701 gtcatcttgc cagacgaagt cttgaaagca gcggtggatt attctgttca atacattcct
    14761 caacgtagct tgccagataa ggctattgac cttgtcgatg taacggctgc tcacttggcg
    14821 gctcaacatc cagtaacaga tgtgcatgct gttgaacgag aaatcgaaac ggaaaaagac
    14881 aagcaagaaa aagcagttga agcagaagat tttgaagcag ctctaaacta taaaacacgc
    14941 attgcagaat tggaaaagaa aatcgaaaac cacacagaag atatgaaagt gactgcaagt
    15001 gtcaacgatg tggctgaatc tgtggaacga atgacaggta tcccagtatc gcaaatggga
    15061 gcttcagata tcgaacgttt gaaagatatg gctcatcgct tgcaagacaa ggtgattggt
    15121 caagataagg ccgtagaagc tgtagctcgt gctatccgtc gtaaccgtgc tggttttgat
    15181 gaaggaaatc gcccaatcgg cagcttcctc tttgtagggt ctactggggt tggtaagacg
    15241 gagcttgcta agcaattggc actcgatatg tttggaaccc aggatgcgat tatccgtttg
    15301 gatatgtctg aatacagtga ccgcacagct gtttctaagc taattggtac aacagcaggc
    15361 tatgtgggtt atgatgacaa tagcaatacc ttaacagaac gtgttcgtcg caatccatac
    15421 tctatcattc tcttggatga aattgaaaag gctgaccctc aagttattac ccttctcctc
    15481 caagttctag atgatggtcg tttgacagat ggtcaaggaa atacagtaaa cttcaagaac
    15541 actgtcatta ttgcgacctc aaatgctgga tttggctatg aagccaactt gacagaagat
    15601 gcggataaac cagaattgat ggaccgtttg aaacccttct tccgtccaga attcctcaac
    15661 cgctttaatg cagtcatcga attctcacac ttgactaagg aagacctttc taagattgta
    15721 gatttgatgt tggctgaagt taaccaaacc ttggctaaga aagacattga cttggtagtc
    15781 agtcaagcgg ctaaagatta tatcacagaa gaaggttacg acgaagtcat gggagttcgt
    15841 cctctccgtc gagtggttga acaagaaatt cgtgataagg tgacagactt ccacttggat
    15901 catttagatg ctaaacatct ggaagcagat atggaagatg gcgttttggt tattcgtgag
    15961 aaagcctaag atagaatttt gaggataaaa aagaaggagc cagctgaaaa aaactggttc
    16021 cttttgaggt acgacgggca tgtcgtatag tagaagtgta ttattctagt ttcaatctac
    16081 tatagtagct cagaagtcgg tacttaaacg tgctatatca aaaccagtcc ttgagaaacg
    16141 tggactggtt tcgtgtttgg attattacct tgaacgacat gcgttaaaag ttagttgaac
    16201 cgccgtatgc cgaacggcac gtacggtggt gtgagaaggg ctagagatta tcccctactc
    16261 gattttaaat cacatgacgt tcaaaggcat catctgaaat cccttgttcc aagatgagtt
    16321 ttgcccattc tttagcagag aagaggctgt ggtccttgta gtttccgcaa gattcgatgg
    16381 ttgtccctgg gacatcttcc caagtagtag tttcagcgat ttccttgagc gaatccttga
    16441 taacagctgc gattttagca ctggtgtgac gtccccacat aatcatgtgg aagcctgtgc
    16501 ggcaaccaaa tggtgaacag tcaatcatgc cgtcaatgcg ggtacggatg agtttggcta
    16561 agaggtgctc gatagtgtga aggccggcag tagggataga gtcttcgttt ggttgcacca
    16621 agcgaatatc ataattggag atgatgtctc cttttggtcc tgtttcttcc ccaatcaagc
    16681 gaacataggg tgctttgaca atggtgtggt caagttcaaa actttcgaca ataacttctt
    16741 ttgacatggt aaatcctttc agttttcttc tctcattata tcataaaggt tgttcctgag
    16801 acagagagaa aacctctccg aggctagaga ggttgaaatc tttacttacg atataagcgg
    16861 tcgtattggt agtatgggtc aaaggttacg ttgataccca gtttacgaag gacattcttg
    16921 tcttcatcag tcaagatgat ggttgagtgg gcttcgcttc ctttgaggtt gccgagttct
    16981 tccatagcgc gggcagcatc aggattttct gtagctgtga tagcaagtgc aatcaggatt
    17041 tcatttgaat gaaggcgtgg attgcggcta ccgagatgat cgattttaag accttggatt
    17101 ggcttaacaa cttcaggctc gattagtttt acttctttag cgatgtcagc tgattttttg
    17161 atggcgttga tcaaggcagc ggctgtaggg ccaaagagtt ctgagttctt accagtgacg
    17221 atttccccat ttggcaattc aaaggctagg gctggtccac cagtttcttc tgctttttgg
    17281 cgcgcaacga cagcaacctt acggtctgca ggtgtgatac cgaggtcgtt catgagcaac
    17341 tcaattttct tgacggcagc ttcgccaact ttttcagctt tgaagtcaag aactgtttga
    17401 tagtaacggc ggatgatttc ttgtttagaa gcttcgacag cagcctcgtc atctgtaata
    17461 gcgaaaccaa ccatgttgac acccatatct gtcggtgaag cgtatggtga ttttccgaga
    17521 atacgttcca acatgcgctt gagcactggg aagatttcga tatcacggtt gtagttgaca
    17581 gtggtttctc cataggtttg aagatggaag gggtcaatca tgttgacatc atcaaggtca
    17641 gctgtggcag cttcataagc caagttaact ggatgatgaa ggggaagatt ccaaacaggg
    17701 aaggtttcaa atttagcgta gccagacttt atgccattga tttggtcgtg gtacatattg
    17761 gacatacacg ttgccaattt tccagaaccg ggtccaggag cggttacgac aatcaagttg
    17821 cgactggttt tgatgtagtc gtttttgccc atgccttctg gggaaatgat gtgatccata
    17881 tccgtcggat atcctttgat tggataatga agataagaat caattccgtt tttctcaagt
    17941 tggttgagga aggcatctgc agcgggttgg ccagcgtatt gtgtaatgac aacggaacca
    18001 acaaaaatcc ctaattcatt gaatttgtca atcaaacgaa gaacttcttg gtcataagaa
    18061 atgcccaagt cgccacgtgc tttggaatgt tcaatgttgc tagcattaat ggcaatcaca
    18121 acctcaacct gctctttcaa ttcttgcaag agcttgattt tgttgtcagg ttcataacca
    18181 ggaaggacac gagcagcgtg gaaatcttct aacattttac cgccaaactc taagtagagc
    18241 ttgccgtcaa attggttaat gcgctccaaa atatggtcgc gttgtaaatt caaatactgt
    18301 tcagaactaa aagcttgttt tttcattttt ttacctctgg actctattat aataaaaaat
    18361 tggaagttag gaaactacgg agctaaaaaa taaattaaaa agattaagca aacgcttgca
    18421 caaaatttta aaaagtgcta tcatatacta tagattatta aaataatgag gtaaacagat
    18481 gcaagaaaaa tggtggcaca atgccgtagt ctatcaagtc tatccaaaga gttttatgga
    18541 tagtaatgga gatggagttg gtgatttgcc aggtattacc agtaagttgg actatctagc
    18601 caagctagga atcacagcaa tttggctttc tcccgtttat gacagcccta tggatgataa
    18661 tggctatgat attgctgatt atcaagcgat tgcggctatt tttggaacca tggaggacat
    18721 ggatcagctg attgcagaag ctaagaagcg tgacattcgt atcatcatgg acttggtggt
    18781 taatcatacc tcagatgaac atgcttggtt tgtcgaagcc tgtgaaaata ctgacagccc
    18841 tgagcgagac tactatatct ggcgcgatga acccaatgac ctagattcta tctttagtgg
    18901 gtctgcttgg gaatacgatg aaaagtcagg tcaatactat ctccactttt tcagcaagaa
    18961 acagccggat ctcaactggg aaaatgaaaa acttcgccag aaaatttatg agatgatgaa
    19021 cttctggatt gataaaggta ttggtggttt ccgtatggat gttattgaca tgattggcaa
    19081 aattcctgac gagaaggtag tcaataatgg tcctatgctc catccctatc tcaaggaaat
    19141 gaatcaggcg acctttggag ataaggatct cttgacagta ggggagactt ggggagcaac
    19201 gccagagatt gccaagcttt actctgatcc aaaggggcaa gaattgtcta tggtcttcca
    19261 gtttgaacat atcggtcttc agtatcagga aggtcagcct aaatggcact atcaaaaaga
    19321 gctgaatatc gctaagttaa aagaaatctt caacaaatgg cagacagagt taggagttga
    19381 ggacggttgg aattccctct tctggaacaa tcatgacctc cctcgtatcg tctcaatctg
    19441 gggaaatgac caagaatacc gcgaaaaatc tgccaaagcc tttgcaatct tgcttcatct
    19501 tatgagagga actccttata tctaccaagg tgaggagatt gggatgacca actatccgtt
    19561 tgaaacactg gatcaagtag aagatattga atctctcaac tatgcgcgtg aggctcttga
    19621 aaaaggtgtt ccgattgaag aaatcatgga cagtatccgt gttattggac gtgacaatgc
    19681 ccgtacccct atgcaatggg acgagagcaa aaacgctggt ttctcaacag gtcaaccttg
    19741 gttggcggtt aatccaaatt acgagatgat caatgtccaa gaagcgctgg caaatccaga
    19801 ttctattttc tatacctatc agaaactggt ccaaattcgc aaggagaata gctggctagt
    19861 tcgagctgac tttgaattgc ttgatacggc tgataaggtc tttgcttata tacgtaagga
    19921 tggcgaccgt cgcttcctag ttgtggctaa cttgtccaat gaagagcaag acttgacagt
    19981 agaaggaaaa gtcaaatctg tcttgattga aaacactgcg gctaaagaag tacttgaaaa
    20041 acaggtcttg gctccatggg atgctttctg tgtggaatta ctataaatat tttttgcaga
    20101 aaaatttaaa attgaaatcg tataaaaaca agggaggact gtataaaaga cagaaatcat
    20161 ttgtttttta taaccaaagt ttataaactt tcattcttga aattcaatta actttacaaa
    20221 ttcccactat taaggagaaa gaagatgaac ataaagaagc gtgtccttag tgcaggcctg
    20281 acttttgcat ctgctttgct tttagctgct tgcggccaat caggttcaga tacaaaaact
    20341 tactcatcaa cctttagtgg aaatccaact acatttaact atctattaga ctattacgct
    20401 gataatatag tcaattgaaa caagaacaag acaaaagagc ctcataaaag gtattgcaac
    20461 ttggtaatac ctttttgagg tgctttttga tatgagccca tgttttctca ataggattgt
    20521 actcaggtga gtagggagga agaggtaaaa gtttataccc aaactcttca cacaagagtt
    20581 ctaacttacc cattctatgg aatcttgcat tatccataat aataaccgat ggtgtgttta
    20641 atgttggtaa gagaaatttc tgaaaccaag cttcaaaaaa gtcgctcgtc atcgtctctt
    20701 cgtaagttat tggagcgatt aactcaccat ttgttagacc tgcaaccaaa gaaatcctct
    20761 gatatcttct tccagatact ttgcctcttc ttaactgacc ttttaatgag cgaccatatt
    20821 ctcgataaaa ataagtatcg aatcctgttt catcaatcta aacaggtgct aggtgcttta
    20881 aactattaaa attcttaaga aataaggcta ctttttctgg ctcttgttca tagtaggtgt
    20941 ggttcttttt ttcgagtgta gcccatagct ttgagcgcat agtggatggt agttggatga
    21001 cagccaaatt cagaagctat ttcagtcaaa taagcgtctg
//
LOCUS       DAVKGC010000026        20679 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKGC010000026 DAVKGC010000000
VERSION     DAVKGC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.026"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            34..3135
                     /locus_tag="VW049_000790"
     CDS             34..3135
                     /locus_tag="VW049_000790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9431951.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGVK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQLRDGRLQMIAQEIREAVAERFWIQ
                     VKNHDSDQEMSRILDQYKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNGIVMKT
                     IYR"
     gene            3217..4224
                     /gene="pfkA"
                     /locus_tag="VW049_000791"
     CDS             3217..4224
                     /gene="pfkA"
                     /locus_tag="VW049_000791"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9431952.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4281..5786
                     /gene="pyk"
                     /locus_tag="VW049_000792"
     CDS             4281..5786
                     /gene="pyk"
                     /locus_tag="VW049_000792"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9431953.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATNSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAIEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6518..6743)
                     /locus_tag="VW049_000793"
                     /pseudo
     CDS             complement(6518..6743)
                     /locus_tag="VW049_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7591..8463
                     /locus_tag="VW049_000794"
     CDS             7591..8463
                     /locus_tag="VW049_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9431954.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLDTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8612..8952)
                     /locus_tag="VW049_000795"
                     /pseudo
     CDS             complement(8612..8952)
                     /locus_tag="VW049_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9095..9406
                     /locus_tag="VW049_000796"
     CDS             9095..9406
                     /locus_tag="VW049_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431955.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            9410..10126
                     /locus_tag="VW049_000797"
     CDS             9410..10126
                     /locus_tag="VW049_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9431956.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10255..11070)
                     /locus_tag="VW049_000798"
     CDS             complement(10255..11070)
                     /locus_tag="VW049_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9431957.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKITSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11067..11972)
                     /locus_tag="VW049_000799"
     CDS             complement(11067..11972)
                     /locus_tag="VW049_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9431958.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSIVVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSHLSPAKKVFQVFVQAIDEFFDTGRYLVFGCLFASVIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            complement(12302..12385)
                     /locus_tag="VW049_000800"
                     /pseudo
     CDS             complement(12302..12385)
                     /locus_tag="VW049_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            12429..14558
                     /locus_tag="VW049_000801"
     CDS             12429..14558
                     /locus_tag="VW049_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9431959.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14545..14994
                     /locus_tag="VW049_000802"
     CDS             14545..14994
                     /locus_tag="VW049_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9431960.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLT
                     NSHSKPLKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            15053..15325
                     /locus_tag="VW049_000803"
     CDS             15053..15325
                     /locus_tag="VW049_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9431961.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(15679..15870)
                     /locus_tag="VW049_000804"
     CDS             complement(15679..15870)
                     /locus_tag="VW049_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9431962.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16298..17056
                     /locus_tag="VW049_000805"
     CDS             16298..17056
                     /locus_tag="VW049_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9431963.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            17058..19046
                     /locus_tag="VW049_000806"
     CDS             17058..19046
                     /locus_tag="VW049_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9431964.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMNVVIAVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPTTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNVSEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(19088..19732)
                     /locus_tag="VW049_000807"
     CDS             complement(19088..19732)
                     /locus_tag="VW049_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9431965.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFTAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLLEQLFNI"
     gene            complement(19758..>20679)
                     /locus_tag="VW049_000808"
     CDS             complement(19758..>20679)
                     /locus_tag="VW049_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9431966.1"
                     /translation="EGKSNREIASLLGKVPQTIHTEIKRGTVRQCLGKGRFKEVYSAD
                     YAQQSYENNRKRSVKKSSLTKELKEKILHYHNQKFSPEMMVMAKGVNVGISTIYYWIH
                     HGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQSIEQRLEAINFRLENGHYEIDTVLL
                     TRAKNYCLLVLTDRKSRHQIIRLIPNKSADVVNQALKLILKQHKILSITADNGTEFNR
                     LSNVFSEEHIYYAHPYASWERGTNENHNRLIRRWLPKGTKKMTPKEVAFIEKWINNYP
                     KKCLDYKSPREDFLMANLNLKFSVRNKSRN"
BASE COUNT         6265 a         3794 c         4379 g         6241 t
ORIGIN      
        1 aatatagtga gaaggataga ggagaagtgt aaattgatcg cacaactaga tacaaaaaca
       61 gtctatagtt ttatggaaag cgtcatttcg atcgaaaagt atgtgagagc agctaaagaa
      121 tacggctaca ctcatttggc tatgatggat attgacaatc tttatggcgc tttcgacttt
      181 ctagagatta caaaaaaata cggcattcat cctttgctag ggcttgaaat gacagtgttt
      241 gtagatgatc aggaagtgaa tttgcgcttt ttagctctat ctagtgtggg ctatcagcag
      301 ttgatgaagc tttcgacagc caagatgcag ggggtgaaaa cttggtcagt cctgtcccag
      361 tacctggagg atatcgcggt cattgtgcct tattttgata gagttgagtc gttagaacta
      421 ggctgtgatt actatatagg ggtttatcca gaaacactag caagcgaatt tcatcatcct
      481 atcttacctc tttatcgggt caacgctttt gaaagcaggg atagagaagt tcttcaagtt
      541 ttaacagcga ttaaagaaaa tctaccgctc agagaagttc ccttgcgttc gagacaagat
      601 gtctttatat cagcaagttc tttagagaaa ctattccaag agtgttttcc gcaagctttg
      661 gacaatttag aaaagcttat ttcaggcatt tcttacgact tggatactag tctgaaactg
      721 cctcgtttta atccagctag accagcagta gaggagttga gagagcgtgc tgaactgggg
      781 cttgttcaga aggggttgac tagtaaagaa tatcaagata gactagacca agaattgtct
      841 gttattcatg atatgggctt tgatgattat ttcttggttg tttgggattt gttgcgtttt
      901 ggacgatcga atggctatta tatgggaatg ggaaggggtt ctgcagtagg cagtttggtt
      961 tcttatgcct tagacatcac ggggattgac ccagtagaga aaaatctgat ttttgaacgc
     1021 tttcttaatc gtgaacgcta taccatgcct gatattgata ttgatatccc agatatttat
     1081 cgtccagatt ttatcagata tgttggtaat aaatatggta gtaaacatgc ggcacaaatc
     1141 gttacttttt caacctttgg agccaagcaa gctcttcgag atgttttgaa acgctttggt
     1201 gtgccagagt atgaattatc tgcaattact aagaaaatca gttttcgtga caatcttaag
     1261 tcggcctatg agggcaatct ccagtttcgt cagcaaatca atagtaagtt agaataccaa
     1321 aaagcttttg agattgcttg caagatagag ggctatccaa ggcaaacctc tgtccatgcg
     1381 gctggtgttg taattagtga ccaagattta accaactaca ttcctctaaa gtatggtgat
     1441 gaaattccac tgactcagta tgatgctcat ggagttgagg ctagcggact tttgaagatg
     1501 gactttctgg gactacgaaa tttgaccttt gtccagaaga tgcaagagtt gcttgctgaa
     1561 acagaaggta ttcatctgaa aattgaagaa atcgatttag aagacaaaga aacgttagct
     1621 ttatttgcct ctggtaatac aaaaggtatc tttcaatttg agcaaccagg tgccattcgt
     1681 ctgcttaagc gtgtgcaacc agtctgtttt gaagatgtcg tcgcgactac ttctctaaat
     1741 cgaccgggtg ctagtgacta tatcaataat tttgtggcaa gaaagcatgg gcaggaagaa
     1801 gtgactgttc tggatccagt actggaggat attttggctc caacctacgg cataatgctc
     1861 tatcaggagc aggttatgca ggttgcccag cgatttgccg gatttagtct tgggaaagcc
     1921 gatattttgc gtcgggctat ggggaaaaag gatgcctctg ccatgcatga gatgagggct
     1981 tcctttattc aaggttcatt agaagctggt catactgtgg aaaaagcaga gcaggtcttt
     2041 gatgttatgg agaagtttgc aggctatggt tttaacaggt cacacgccta tgcctactca
     2101 gcattggcct tccagttggc ttattttaaa acacattatc cagccatatt ttatcagatt
     2161 atgttaaatt cttccaacag tgattactta atagatgcac ttgaagcagg ttttgaagta
     2221 gcctctctat ccatcaacac cattccctat cacgataaaa ttgccaacaa ggccatctat
     2281 ctaggtttga aatccattaa aggagtcagt aatgatttag ctctctggat tattgaaaat
     2341 agaccttatt ctaacattga agattttata gctaaattac ctgagaatta tctgaaactt
     2401 cctctgctag aacctttggt aaaagttggt cttttcgatt catttgaaaa aaatcgtcaa
     2461 aaagtattta ataacttagc taatctattt gaatttgtga aagagttggg aagtttgttt
     2521 ggagatgcta tttatagttg gcaggaatcg gaagattgga cggaacaaga aaaattttat
     2581 atggaacaag agcttttagg gataggtgtc agcaaacatc cactacaagc tattgcaagt
     2641 aaggctattt acccgattac cccaatcgga aatttgtcag aaaatagcta tgctattatc
     2701 ttggttgaag ttcagaaaat aaaagtgatt cgtaccaaaa agggtgaaaa tatggccttc
     2761 ttacaggcag atgatagtaa gaaaaaattg gatgtcactc tcttttcaga cttatatcgt
     2821 caggttggac aggaaataaa agagggagcc ttctactatg taaaaggaaa aatacaatta
     2881 cgtgatggcc gtctgcaaat gattgcacaa gaaataagag aagcagttgc tgaacgcttt
     2941 tggattcagg ttaaaaatca tgactcggat caagagatgt cacgtatttt agaccagtat
     3001 aaaggcccaa tcccagtcat catccggtat gaagaggaac agaaaaccat cgtttctccc
     3061 catcattttg tagctaaatc tgatgaatta gaggcgaaat tgaatggaat agttatgaaa
     3121 acgatttatc gctaaaatta cggaaaatag aagaattttc aacgtaaatg tggtataatc
     3181 agtaagaatg ttaaaagaaa aaggagcata accaatatga aacgtattgc tgttttgact
     3241 agtggtggag acgcccctgg tatgaacgct gccatccgtg cagttgttcg tcaagcaatt
     3301 tcagaaggaa tggaagtgtt tggtatctat gacggatatg ctggtatggt tgccggtgaa
     3361 attcatcccc tagatgcagc ttcagtaggg gacatcattt ctcgtggtgg tactttcctt
     3421 cactcagctc gttacccaga gttcgctcaa cttgaagggc aacttaaagg gattgagcaa
     3481 ttgaaaaaac acggaattga aggtgtagtt gttatcggtg gtgacggatc ttaccacggc
     3541 gctatgcgtt tgactgaaca tggcttccca gctattggtc ttccaggtac aatcgataac
     3601 gatatcgttg gtactgactt tacaatcggt tttgacacag cggttactac tgccatggac
     3661 gctatcgata agattcgtga tacatcatca agtcaccgtc gtacttttgt aatcgaagtt
     3721 atgggacgta acgctggtga tatcgctctt tgggctggta ttgcaactgg tgctgatgaa
     3781 atcatcatcc ctgaagcagg cttcaagatg gaagatatcg tagcaagcat caaagctggt
     3841 tatgaatgtg gtaaaaaaca caatatcatc gtcttagctg aaggtgtgat gtcagcggct
     3901 gaatttggtc aaaaacttaa agaagctgga gatacaagcg accttcgtgt aacagaactt
     3961 ggacatattc aacgtggtgg ttctccaact gcgcgtgacc gtgttttggc gtcacgtatg
     4021 ggtgcacatg ctgttaaact tcttaaagaa ggtatcggtg gtgttgcggt tggtattcgt
     4081 aacgaaaaaa tggttgaaaa tccaattctt ggtactgcag aagaaggggc attgtttagc
     4141 cttactgcag aaggtaagat tgtggttaac aacccgcaca aagccgatat tgagctatct
     4201 agcttgaata agagcttgtc ataatttaca atttagttaa taagaccaaa aaggtcatat
     4261 ataaaggagt cacaaaaatc atgaacaaac gtgtaaagat cgttgcaact ttgggtcctg
     4321 cggtagaaat ccgtggtggt aaaaaattcg gtgaggacgg atactggggt gaaaaacttg
     4381 atgttgaagc ttcagctaaa aacattgcta aattgattga agctggtgct aacacattcc
     4441 gattcaactt ctcacacggc gaccaccaag aacaaggtga gcgtatggca actgttaaac
     4501 ttgcggaaaa aattgcaggt aaaaaagttg gtttccttct tgatacaaaa ggacctgaaa
     4561 tccgtacaga attgttcgaa ggtgaagcta aagaatattc atacaaaact ggtgaaaaaa
     4621 ttcgtgttgc aactaaacaa ggaatcaaat caactcgtga agtgattgcg ttgaacgttg
     4681 ctggtgctct tgatatctat gatgatgttg aagttggtcg tcaagttttg gttgacgatg
     4741 gtaaacttgg tcttcgtgtg gttgctaaag atgatgcaac tcgtgaattt gaagttgaag
     4801 ttgaaaacga tggtatcatc gctaaacaaa aaggtgtgaa catccctaac actaaaattc
     4861 ctttcccagc tcttgctgaa cgcgataacg acgatatccg tttcggtctt gaacaaggta
     4921 tcaacttcat cgcaatttca ttcgtacgta ctgcaaaaga tgtgaacgaa gttcgtgcaa
     4981 tctgtgaaga aactggaaac ggacatgttc aattgttcgc taaaatcgaa aaccaacaag
     5041 gtatcgataa cttagacgaa atcatcgaag cagctgatgg tattatgatt gctcgtggtg
     5101 atatgggtat cgaagtaccg ttcgaaatgg ttccagttta tcaaaaaatg attatcaaga
     5161 aagtcaatgc tgcaggtaaa gttgttatca ctgcaacaaa catgcttgaa acaatgactg
     5221 aaaaaccacg tgcaactcgt tcagaagtat cagacgtatt caacgctgtt atcgacggaa
     5281 ctgacgctac aatgctgtca ggcgagtctg caaacggtaa atacccactc gagtcagtaa
     5341 ctacaatggc tacaatcgac aagaacgctc aagctcttct taatgaatac ggacgtcttg
     5401 attcagattc atttgagcgt aactctaaga cagaagtaat ggcttctgct gttaaagatg
     5461 ctactaactc aatggatatc aaattggttg taactcttac taagacaggt catactgcac
     5521 gtttgatttc taaataccgt ccaaatgctg acatcttagc attgacattt gacgaattga
     5581 cagaacgtgg cttgatgttg aactggggtg ttatcccaat gttgacagat gctccatctt
     5641 caactgacga tatgttcgaa atcgctgaac gtaaagcgat agaagcaggt ctcgttgagt
     5701 caggcgatga tatcgttatc gttgctggtg tgccagtagg agaagctgtt cgcacaaaca
     5761 caatgcgtat ccgcacagta cgttaagaaa aatataaaaa cctatcatat ccagctttag
     5821 agcttgtgtg ataggctttt tgtatagagg gtaagaaata ggcaaaactt tcataatgga
     5881 ttgatactct tcgaaaatca aattcaaacc acgtcagcgt cgccttaccg tatatatgtt
     5941 actgacttcg tcagttctat ctacaacctc aaagcagtgc tttgagcaac ctgcggctag
     6001 cttcctagtt tgctctttga ttttcattga gtatgaaata agatatgcac aaattgatta
     6061 ggaaattcaa atgaatttct acaaatgttt tagcaatcgt aatgtacttg tctagattcg
     6121 atctgatata ttttcgattt aatgatatgg tatttaaaac ctccaaagta gcttgctcca
     6181 ttcttttact tacgtgagtg tagatgttat ttactgtttt agcgtttttg tgttccactc
     6241 taaccattat agcattcttc tcagctagtg tactaaggag tgtgtgcctg aaaatatggg
     6301 aactaagggg ctggtttatc ggtttctcta gtttagtatt tgccttttgc aaagtgatct
     6361 taaatgcctt tctctaaatt tacatatcac tattgtttaa caaaatctaa tctattttag
     6421 gtcacttatt ctttttttga aatgtagaat gaactttttc aaagtttttc gaatctttta
     6481 aaatctgttt gttttatatc gccattctcc ccctttttta attctcccta tatagcctga
     6541 caactttccc gatggtacga atatggttgc tttcgtctag gtggatgtcg gagtattcgg
     6601 gattgagttt ttttgaggta gccttggcgg agtttcttga catagttagt gccgtctact
     6661 tggaagatgc cgatggtatt atagtcaatc tgtggggtat tcttgataaa taggtagtcg
     6721 ctgtttctta tctttggctc catggacttg ctgacgacat aagcgattgg gtcgtagtcg
     6781 tctgggataa tggaaactcc ataactaaat cgtcgtcctg catcaagcgg ctacctgcag
     6841 agataaacta cctaacacga gagtaagtag tctgtctgta gtcgtccagt ctgatgattt
     6901 ttacgatact tcgtttttct gatcatacag ttgcctctcg gcataggtca gaactttacc
     6961 ttgtctgggt ggtgcccgtt ggtcgtagat agattggata tcgctaggag aatccttttg
     7021 aactggagga aagagggcat caatcaagct actgaatact ttaactaagt caaatatagt
     7081 atttttctta gtagacctaa cccttttttc ataatttcca atggtgtttt tacttatacc
     7141 aatcttagta cccaattctt attgagtcca accattacta gtctatattg ttttatagtt
     7201 gattgagttt ggaatagtac gctgtagctg ctaaaacatt tctaggaatt aatttgcctt
     7261 tcctaataga gttgttcata tcttatttca atctactata tttttcgcct ctaacaatcg
     7321 caatctcttc tttatctacg aatgaaatcg ctttctattt ttgtaagtaa agcatcacac
     7381 gaaatccacg aaaatgaaaa cctttgttgt gttttcgtaa aaaatttgtt gacagagcac
     7441 gaaacgccta ctataattaa atcaagctta aggaaataac aaaaaaacaa atcggaggga
     7501 aagacgatga acatattaaa tgagaaagta atcaatatct gtaaagcagt agttaaagaa
     7561 actttaatcc aagacattta ggagaaagaa atgaagaaat caagaaaatt agccacatta
     7621 ggaatctgtt cagctttgtt tttaggcttg gctgcttgtc aacaacaaca tgctacttct
     7681 gaggggacga atcaaaggca aagcagttca gcgaaagttc catggaaagc ttcatacacc
     7741 aacctaaaca accaggtaag tacagaagag gtcaaatctc tcttatcagc tcacttggat
     7801 ccaaatagtg ttgatgcatt ttttaatctc gttaatgact ataataccat tgtcggctca
     7861 actggcttat caggagattt cacttccttt actcacaccg aatacgatgt tgagaaaatc
     7921 agtcatctct ggaatcaaaa gaagggcgat tttgttggga ccaactgccg tatcaatagt
     7981 tattgtcttt tgaaaaattc agtcaccatt ccaaagcttg aaaagaatga ccagttgctt
     8041 ttcctagata atgatgcgat tgataaagga aaggtctttg attcacaaga taaggaagag
     8101 tttgatattc tattttcgag agttccaact gaggcaacta cagatgtcaa ggttcacgct
     8161 gaaaagatgg aaacattctt ctcacaattt caattcaatg aaaaagctcg aatgctgtct
     8221 gtagtcttgc acgacaatct ggatggcgag tatctgtttg taggccacgt tggggtctta
     8281 gtacctgctg atgacggttt cttatttgta gagaaattga ctttcgaaga gccctaccaa
     8341 gcgattaaat ttgctagtaa ggaagattgc tacaagtatt tggacaccaa gtatgcggat
     8401 tatacaggcg agggactggc taagcctttt atcatggata atgataagtg ggttaaactt
     8461 taatagtgtt atgatatgac agtaatctta ggatttttat ttctattttt gatgagttgg
     8521 tgggttattt caatcgttaa tagccagaaa ccacgcaaaa atgaaacatt tattggctat
     8581 gtccaaagat acgatattga tgggaatggc tatcaatcat taccgtgtcc tcagaactga
     8641 gaggagttct tgaaatcgta aaaccacttt gaacaagagt tacttcaacc cattggctcc
     8701 gacggattaa gttgctttcg tgaataccaa aatcagccgc aatttcttca taagtgcggt
     8761 attctcgcac atattgaaag tggccataag aaggtcttct aggcttaatt taggttttcg
     8821 tccacctttt gcgtgtttaa gttgataagc tgtttttaat acagctaaca tctcttcaaa
     8881 agtcgtgcgc tgaacaccaa caagacgctt aaatcgtgca tcagttagtt gtttacttgc
     8941 ttcataattc atagaactat tataccatgt tttgtttcgc aggaaatcta atgctaaaat
     9001 cttatcagtg agaagatttc ttaaagttta ttctttgtag tttcatctgt attagtacat
     9061 aatttaactt agagaaaata ggaggactaa tcgaatggaa ttaaaagatt ttacagaaaa
     9121 agaacaggaa atgattaaga aaaggcttac aatgtctaat attagtgaca aagaaactac
     9181 tgagaagatt ctggcgctag tcccacaaga cttgattaag cgaatcccgt tttttgtcag
     9241 aaaacatgct acaacacgta cgattaaacg catttcaatt gaataccctg aactctacgc
     9301 tgtagctcaa acaagtggtg agattccaga aaaaaaacgc gaagaattgc gtcagattat
     9361 cacaactatc tttgaacaaa agatgaataa gcatagtatt aagtagagaa tgaaaaaata
     9421 ttttattggc ggtttgggaa gcaatgccta tcatagcaag gattttcttc aagaactaga
     9481 ttcgcaggtc tattttctaa atccatatga aaagcatctt cgagatgaaa cagaattgaa
     9541 atcatggttt aaaaatgaaa ttgtagagga agaatctatc tgtctgatag gccattctct
     9601 tggaggagat ttagctcgtt atttcgcatc ggaatttgaa gaagtaaaga aactgattct
     9661 tttggatggt ggctatctag atttagataa gattttacct ttggatacag agttagagga
     9721 aactaaaaat tatatcaaat ctcaaattgt ttcggactta gatgttctta cttctaaaga
     9781 aaaatctgaa gcaaagcatt ggtcagaaaa tatggagaaa gctgtaagac agtcttatca
     9841 ctggaatgtt gagtataata gatatgagtt ggctataaat tatgaaaata tagaagcgat
     9901 actccgccta cggagaaaaa tacaagcttt taagagagaa gtgggagata ccttgtttat
     9961 tagtcctcgc tatcctaatg aagctacatg gagagaggaa gccctaaaag aattgccaga
    10021 ctattttgat actatttttt tagaaaactt tggccatgag ctttatactc aagcacctaa
    10081 agaaatcgct agtctaatga atgagtggct cgcttatttt ctatgagcta cttgtattgc
    10141 tgggaatggt ataagatgaa aatctacaag tcactaattt tcatataaca aaaaacgaac
    10201 aggctctttt tctgttattc agaagagaac ttgttcgttt ttatcttgat tttcttaaaa
    10261 agctctatat acatagggat tttctggttt atcgactttg gtaaagctgt cgatttccaa
    10321 ggttggaagg ttttgtttga gttctttatg gtagtgattg accagttttc ctttggctgt
    10381 tatccaagtg ttgttttcat actgccgggt attgcccttg gtcagcaatc catagacacc
    10441 agaatctgcg atacagtgga taatgccgaa tcggaacaga aattgactat tggcatgact
    10501 ggggtcgtta tagacaaagc ctgtaaactg gattgtcttg ccctcaaact catctggata
    10561 gtcgtagata gcctccatga cttccatata gttttcatta gtgatctgaa tactatcttg
    10621 ggataagtat ttatccgccg ccgttcgcat ttccttttca taggctgatt ttgaaaaata
    10681 agaactggta tctggtttca aatattggct tgtcgtccct tcgcttgtct gaatggctag
    10741 atccgttcct tccgataggg ggaaatgata acctttagca gaaacagtct gagaatccaa
    10801 gctaacagtt gggaaagtta agccgatgac aagtggaata gccagaagag aaatactcgt
    10861 tatcttggct aatcggctgt tcagatgact gtgggttttg acttgcttca tccagatata
    10921 caattgaacg atagccaaga taaaagaaag caccatggag atataggcca gataggaata
    10981 gtgcatgttg atgtactggt ttagtttgcc cgacagatgg aggtaaatag tcagttcaaa
    11041 atagccagct aaaactaaaa atcgaatcat aggataactc caatcaagag agaatagact
    11101 aagacgacaa gagttacaat tgttatgaag tgactgataa atcgtgcttt caagtaattt
    11161 ttcatcatga gaatattttt gatatccagc attgggccaa tgacgagaaa agccagaact
    11221 ggagccaaac cgaaactcga gagtagagaa gccccgataa aggcatccgc ctcactacag
    11281 agcgaaagaa gaaaggccaa aatcatcaag agcaggatgg caaaaagagg agtcgcactg
    11341 atagaggtca gaatccgagt cggaacataa acctgtataa cagaggcaaa gagacagcca
    11401 aataccaaat aacgtcccgt atcaaaaaac tcatcaatgg cttgcacaaa gacctgaaaa
    11461 actttttttg caggacttaa gtgagaaaaa tcatgctcat gacaggctag acgattttct
    11521 ttttgaatcg gttcttgcca gaaaaatcct agaaaaattc ctagtattac agccacaacg
    11581 atggaaccca gagctcgtaa tagggcgaca tggaaggagt tgccaaaggc agaataggtc
    11641 gcaaaaagaa caatgggatt gataacaggt gctgtcacaa gaaaaggaac ggccgtgtaa
    11701 cttggaacct ttttttccag aaaacgattg atgatgggga cgattccaca ttcacaggaa
    11761 gggaaaagta taccgacaaa ggtcccaaaa aagattctcc cccaacgatt tcgagggaga
    11821 aaatgataaa ccttgtcagg tgtgatataa acttcaatca gccctgagac aatacttcct
    11881 atcagaacga agggaagggc ttcaatgata atggagagaa aaatggctcc agtttgaagt
    11941 acactagatg ggagttgttg aaaagcagtc atctagtttt tcttttctgc tttggctttt
    12001 ttctctttgt catcagggaa agtgacttgt ttcaaatcag ggagtttagc aaactcttca
    12061 aacatcttgt ccaagtcgtc tcgttttgta aatttgacag ccataggtgt acctcgattc
    12121 taaattctac ctctattata ctattatttt gcggaaaagg gagtttttta atttgttttc
    12181 gatagttgat actcttcgaa aatctcttca aaccacgtca gcttcgcctt accgtagtac
    12241 ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag ctgacttcgt
    12301 cagttctatc cacaacctca aaacagtatt ttgagcagcc tacggctagc ttcctagttt
    12361 gctttttgat ttttattgag tatgaaaagg attggagaaa gccttccagc ttgtggtata
    12421 atagatttat ggataaaaaa tatgaaaaaa tctctcagga tttgggagtg acgttaaagc
    12481 aaattgatac cgttctaagt ttgacagctg aaggggcgac tattcccttt atcgcgcgtt
    12541 atcgcaagga catgactggt agtctggatg aggtggcgat taaggctatt attgatttgg
    12601 ataaaagtct gacaaatctc aatgaccgta aggaagctgt cttagctaag attcaagaac
    12661 aaggtaagtt gaccaaggaa ttggaagaag ctatcttagt tgccgaaaaa ttagcagacg
    12721 ttgaagaact ctatcttcct tataaggaaa agcgtcgtac caaggcaacc attgcccgtg
    12781 aagctggact ctttcctctt gctcgtttga ttttgcagaa tatagttgac ttagagaaag
    12841 aagctgaaaa gttcgtctgt gaaggatttg cgactggcaa ggaagccttg accggtgcag
    12901 ttgatatttt ggtcgaagcc ttatcggaag atgtgacctt gcgttctatg acttatcagg
    12961 aagtgctgag acactctaaa ctcacttctc aagccaagga tgaaagtctt gatgaaaagc
    13021 aggtttttca gatttattat gatttttcag agacagttgg aactatgcaa ggctatcgta
    13081 ccttggctct caatcgtggg gagaaacttg gtgtcttgaa gatcggtttt gaacatgcga
    13141 cggaccgtat tcttgccttc tttgctactc gtttcaaggt gaaaaatgct tatattgatg
    13201 aagttgttca gcaatccgtt aagaaaaagg tcttgcctgc tattgagcgt cgtattcgga
    13261 cagaattaac tgagaaagct gaagagggag ctatccaact tttttctgac aatctgcgca
    13321 atctcctctt ggttgctcca ctgaaagggc gcgtggttct tgggtttgac ccagcctttc
    13381 gtacaggtgc caagctagct gtcgtggatg caacaggaaa aatgctgaca actcaggtta
    13441 tttatcctgt taaaccagca tcagctcgtc aaatcgaaga agccaagaaa gatttagcag
    13501 atttaattgg tcaatatggt gtagagatta ttgccattgg aaatggaacg gccagtcgtg
    13561 aaagtgaagc ttttgtagcg gaagttctga aagatttccc tgaagtcagc tatgttatcg
    13621 ttaatgaaag tggtgcttct gtctattctg ccagcgaact tgctcgtcag gagtttccag
    13681 acttgaccgt tgaaaaacgc tctgccattt ctatcgcccg tcgtttgcaa gatcctcttg
    13741 cggaattggt caaaatcgat cctaagtcaa ttggtgtcgg tcaataccaa cacgatgtca
    13801 gtcagaagaa actatctgag agtctggact ttgttgtcga tacagtggtt aaccaagttg
    13861 gtgtcaatgt caatacagct agcccagctc ttctttcaca cgtagctgga ctcaataaaa
    13921 ccatttctga aaatatcgtc aaataccgtg aggaagaggg aaaaatcact tcacgctccc
    13981 aaatcaagaa agttcctcgt ttgggggcca aggcttttga acaggctgct ggtttcctcc
    14041 gtatccctga aagtagcaat atccttgata atacaggagt tcacccagaa aattacgctg
    14101 ccgttaagga actcttcaaa tgcttggaca ttaaggatct gaatgaagag gcccaaagca
    14161 aactcaagtc gctttcagtc aaggaaatgg cgcaagagtt agaccttggt ccagaaactc
    14221 ttaaagatat cattgcagat cttctcaaac caggtcgaga tttccgtgat tcctttgatg
    14281 ctccagttct ccgtcaagat gtcctagata tcaaggactt agtggttgga caaaagctag
    14341 aaggagtggt gcgtaacgtt gttgatttcg gtgcttttgt tgatatcgga attcatgagg
    14401 acggtctcat ccatatttcc catatgagtc gcaagtttat caaacaccct agtcaagtgg
    14461 tggcagttgg agatttggtg agcgtttggg ttaagaaaat tgatactgaa cgtgaaaaag
    14521 ttaatctgtc tctcctagct ccaaatgaaa ctgactgatt acgtcaagca ggtttcacta
    14581 gaagacttcg gcagaccttt tatccatcat gtccagtgga ataggcgtct acgttcgaca
    14641 ggtgggcgat ttttccccaa agatgggcat ttggatttta atcccaaggt ttatcaggaa
    14701 ctggggttgg acgtttttag gaaaattgtc cgacatgaac tctgtcatta tcacctctat
    14761 tttcaaggga aaggctatca acacaaggat cgggatttta aggaactgtt gaaagcagtg
    14821 gatggattac ggtttgtacc atccttgaca aatagccact ctaaaccact caaactttat
    14881 cgttgccaat cctgccagca aacttatcag cgtaagcgta ggattgatac caaacgctat
    14941 cgctgtggac tttgtcgagg aaagttgcta ttagtaaatc agcctgagga ctgatgaaag
    15001 ccgagcctat ccgtgatata ctatgtctaa cctagcagaa agaggaaata tcatgaacag
    15061 taagttttat aaaatgagac gaaatcgcat gatttcagga gttttagcag gtctgtcaga
    15121 caagtggaac tttgatgtta cccttgtacg ttttctgttt gccattttta cagtcgcaaa
    15181 ctttggactt ggggtgatta tctatatcat cctagcttct atcatgccaa ccaaggaaga
    15241 aatcgaagca gaaatgtacg gaacaggccc acgtaaacgc aaagaagctc aagccattga
    15301 cgataatgaa ggctggtttt ggtgaggttt tacaaaagat ttggaatacg caaaaagctt
    15361 taaatcaata gtctaaactg cttgatttaa agctttttta cttttatact caataaaaat
    15421 caaagtgcaa actaggaagc cagccgcagg ctgtaattta gtcggcaatg tgaagttgat
    15481 atagtttgca ctttgatttt tgaatagtat taataatatt tcgccagctt catccacaaa
    15541 ctatagtgga ttggaactag aatagtacac ctctacttct aaaacattgt tagaaatcga
    15601 tttgactatc ctgatcgatt tgtcctgttc ttatttcatt ttattataaa agcagtaatt
    15661 taaactcagt tttttaatct atttttgctg ctgagacaat acatttttgg aaaaatggtg
    15721 gatgctgata acaactgata accagcaaat aacataagca gtgccggtaa attctgtacc
    15781 gataaagtgt gtgtgttcaa ctaagagctg accgatgaat ggagagagca gatagagaag
    15841 tccgatgagt atatgttctt tgtgtttcat taaagcgcac cttctttcct tataggttga
    15901 acgacttgca agtgttaaat tgaatggtat gttatctagg taaccttact aaaatgttag
    15961 ttcccgcttc ctccttttca aagtaagcct tttcacactt atattttaca actttaaaaa
    16021 taggaaatca aatcattttc taagtaattt tgcttatatt tcacccaatc ttacaaaaat
    16081 gtaagattaa agtctctttt gtaaggttag gttatggcaa gcggtctttt tttgatgtaa
    16141 actagactca taaagaacaa aggagtaaca tcatgaaaca aatcttacat aagaaaacta
    16201 gaaaaccaag tcacaaagaa attgagcgcg ttcagctggg atgcactatg atgcaagcta
    16261 catttcattt gatgggatat taagaaagga gattctcatg acacttttag atgtaaaaca
    16321 cgttcaaaaa atttataaaa cacgttttca gggcaaccaa gtagaagccc tcaaggatat
    16381 tcactttacc gtcgaaaagg gtgactacgt tgccatcatg ggtgagtctg gttctggtaa
    16441 atcaactctt ctcaatattc tagctatgct ggataaacca agtcgtggtc aggtttactt
    16501 gaatggaact gacaccgcaa ccattaaaaa ttcacaggcc tctagcttcc gtcgtgaaaa
    16561 gttgggcttt gtcttccaag actttaactt gctagatact ctatctgtaa aggacaatat
    16621 cttgcttccg cttgtcttgt caagaagacc tattacagag atgatgaaga aattggtggt
    16681 gacagctgag aatctgggta ttaaccaatt gcaagagaag tacccttacg agatttctgg
    16741 tggccagaaa cagcgtgtag cagtagcacg cgccatcatc actgaacctg aaattcttct
    16801 tgcggacgaa ccgacaggag ctcttgactc caagtcatct gcagccttac ttgatgtctt
    16861 tgatgaaatc aatgagcgcg gccaaaccat tctcatggta acccactcaa cagcagctgc
    16921 tagcagggcc aagcgtgttc tctttatcaa ggacggcatt ctttacaacc aaatctaccg
    16981 tggagagaag acagagcgtc agatgttcca agaaatctct gataccttga ctgtcatggc
    17041 aagcgaggtg aattagtatg tttcgattaa ccaataagtt agcggtatcg aacttgatta
    17101 aaaaccgcaa actctactat ccttttgcgc tggctgttct cttggcagtc actgtcacct
    17161 atctctttta ctctctaacc ttcaatccca agattgcgga aatccgtgga ggaacaacca
    17221 ttcaggctac acttggattt ggtatgtttg tcgtcaccct tgcgtcagcc attatcgttc
    17281 tctatgccaa tagttttgtc atgaagaacc gttccaagga actaggaatt tatggcatgt
    17341 tgggcttgga gaagcgccat ctaatcagta tgacctttaa ggagttagtg gtatttggga
    17401 ttctaactgt tggagcgggt atcggtattg gagccttgtt tgacaagtta attttcgctt
    17461 tcctgctcaa actaatgaaa ctgaaggttg agctggttgc taccttccaa atgaatgttg
    17521 tcattgcagt acttgttgtc tttggattga ttttcctagg cctcatgttc ctgaatgctc
    17581 ttcgaatcgc ccgtatgaat gccctccagc tctcgcgtga gaaagcaagc ggagagaaaa
    17641 gaggtcgctt cctacctctc caaacgattc ttggttccat aagtttaggg attggctatt
    17701 atcttgccct tacggtaacc gatcctctta cagccctaac aactttcttc ctagctgttt
    17761 tgctggttat ctttggtact tatctattgt ttaatgcagg gattacagtc ttcctacaaa
    17821 tcttaaagaa aaacaagaaa tactattacc aacctaataa cctcatatct gtttccaact
    17881 tgattttccg tatgaagaaa aatgcggttg gactagcaac catcgctatt ttgtcaacaa
    17941 tggttttggt aaccatgtca gcagcgacaa gcattttcaa ttccgcagaa agctttaaaa
    18001 aagttctaaa tcctcatgat tttggggttt cagggcaaaa tgttgaaaaa gaagatttgg
    18061 acaaactctt gagccagttt gcaagtgaca aaggttatag tgtcaaagag aaagaagtac
    18121 ttcgttacag taactttggt attgcaaatc aagaaggaac caagttaact atttttgaaa
    18181 aaggacaaaa ccgtgtccaa cccacaacag ttttcatggt atttgaccaa aaagattatg
    18241 aaaatatgac tggtcaaaaa ctgtctctat caggaaatga ggtcggtctc tttgccaaaa
    18301 atgacggact gaaaggacag aaagctctaa ctctaaatga tcatcaattt tctgtcaaag
    18361 aagaatttaa taaagatttc attgtcaacc atgttccaaa taagtttaat atcttgacta
    18421 ctgattacaa ttaccttgtt gttcctgatt tacaagcctt tttggatcaa ttcccagatt
    18481 cggctatcta taatcagttt tacggtggta tgaatgtaaa tgtcagtgaa gaagaacaac
    18541 tcaaggtcgc tgaggagtat gaaaactacc tcaatcaatt taatgctcaa ttagacacag
    18601 aaggtagcta tgtttatggt agcaatctag cagatgctag ttctcagatg agtgccctct
    18661 ttggtggtgt cttctttatc ggtattttcc tatccattat ctttatggtc ggaactgttc
    18721 tggtcatcta ctacaaacaa atttctgaag gctacgaaga ccgtgaacgc tttattatct
    18781 tgcagaaagt cggtttggac caaaagcaaa tcaagcaaac catcaacaaa caggttttaa
    18841 ctgttttctt ccttcctttg ctctttgcct tcatacatct cgcctttgcc taccatatgc
    18901 ttagcctgat tttaaaagtg attggtgtac tggatacgac tatgatgttg attgtgacct
    18961 tgtctatctg cgctatcttc ctcatcgcct atgtgctgat tttcatgatt acttcaagaa
    19021 gttatcgcaa gattgtgcaa atgtaaaaaa gatacctcga cttcaaaatc gaggtatttc
    19081 ttgtattcta aatgttgaaa agttgttcga gcaggaaggt aactcccatg gtcaagagac
    19141 caatagcaag gttccgaatc atagctgttc tagtcggatc ctttccaagt ctagcacttg
    19201 tgtaaccagt gagaagaagg gccacaccga caataaggac ggtagcaggg atgcggtaat
    19261 cacttggaaa aatggtcact gacagcattg gaggcaaact tctaaggaaa aaggcaacga
    19321 agctagaaat ggcagcgtgc caaggattgg taaattcttc atactcaatc ccatattttt
    19381 cctctaccag agccttgagt ggatttttaa gaaagatctt attggtcaag agttgggcag
    19441 aagttttgaa ttctccattt tggatataag cagcatagag ggattttttg gctagttccc
    19501 tatcttggtc tagcaagagt ttttctcgcg aaacggcagc ttcctcggta tcttttggag
    19561 ttgaaacgga tacatattct ccaccagcca ttgaaaaggc accagctaag atagccgtaa
    19621 aacctgataa aaagataatc cagatattgg tcgtggcact ggcaactccg ataaccacac
    19681 cagcaatgga aataattcca tcgttagcat caagaacacc cgcacgcagg atatttaaac
    19741 gacctgcaaa atttgaatca atttcgtgat ttgtttctga cgctaaattt caagttcaag
    19801 ttagccatca agaagtcttc tctgggtgac ttgtagtcca agcatttttt aggatagttg
    19861 ttaatccact tttcgatgaa tgcgacttct ttgggagtca ttttcttggt tcccttaggt
    19921 aaccatctac gaatgagcct gttgtgattc tcattagttc ccctttccca agaggcatag
    19981 ggatgtgcat aataaatgtg ctcctcagaa aatacattag acaagcgatt gaattccgtt
    20041 ccattatctg ccgtgatgga aagaatcttg tgttgtttta agatgagttt tagagcctga
    20101 ttgaccacat cagcactttt atttggaatc aatcggatga tctgatgtct actttttcga
    20161 tccgtcaaga caagcaagca gtagtttttc gctctcgtaa gtagaactgt atcaatctca
    20221 taatgcccat tctccaagcg aaaattgata gcttcaagcc gctgttcgat ggattgacca
    20281 gcaggtttaa agttggtgct ggcctgtttc ttaagcgctt ttccttttct agggtaaagc
    20341 agatcctgtt tgcttaaccc caattttcca tgatgaatcc aatagtaaat ggttgaaatt
    20401 cccacgttaa cccctttagc catcaccatc atttcaggcg aaaatttttg gttatgatag
    20461 tggagaatct tttcctttag ttccttggtc aagcttgatt tcttgaccga gcgcttgcga
    20521 ttgttttcat aagactgttg agcatagtcg gcagaataaa cctctttgaa gcgccctttt
    20581 ccaagacatt gtcggactgt cccacgcttg atttcagtgt ggatagtttg aggaactttt
    20641 ccaagtagag aggcaatttc tctatttgat ttcccttct
//
LOCUS       DAVKGC010000027        20637 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKGC010000027 DAVKGC010000000
VERSION     DAVKGC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.027"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            142..2124
                     /locus_tag="VW049_000809"
     CDS             142..2124
                     /locus_tag="VW049_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9431967.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSAKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEEFNKKAQEDLAKHVK"
     gene            2425..7728
                     /locus_tag="VW049_000810"
     CDS             2425..7728
                     /locus_tag="VW049_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9431968.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPVAAKPETPKTVTPEWQTVEKKEQQGTVAIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGTTTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDEAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWIDSKKAKKVTFLVTGAETGD
                     TWVGIYSTGNASNTRSDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKSKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(7895..10054)
                     /locus_tag="VW049_000811"
     CDS             complement(7895..10054)
                     /locus_tag="VW049_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEU9431969.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10051..10647)
                     /gene="recU"
                     /locus_tag="VW049_000812"
     CDS             complement(10051..10647)
                     /gene="recU"
                     /locus_tag="VW049_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9431970.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10713..11240
                     /locus_tag="VW049_000813"
     CDS             10713..11240
                     /locus_tag="VW049_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9431971.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11310..11639
                     /gene="gpsB"
                     /locus_tag="VW049_000814"
     CDS             11310..11639
                     /gene="gpsB"
                     /locus_tag="VW049_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9431972.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11660..12040
                     /gene="rnpB"
                     /locus_tag="VW049_000815"
     ncRNA           11660..12040
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW049_000815"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12125..13282
                     /locus_tag="VW049_000816"
     CDS             12125..13282
                     /locus_tag="VW049_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9431973.1"
                     /translation="MKKEFNLIATAAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13295..14665
                     /gene="mapZ"
                     /locus_tag="VW049_000817"
     CDS             13295..14665
                     /gene="mapZ"
                     /locus_tag="VW049_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9431974.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDANAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQASSNTTS
                     EPKPSSSNETRSSRSEVNMGLSSEGVAVQRSASRVAYNQSAIDDSNNSAWDFADGVLE
                     QILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGYFVGNGAGH
                     ADDLDY"
     gene            14741..16165
                     /gene="gndA"
                     /locus_tag="VW049_000818"
     CDS             14741..16165
                     /gene="gndA"
                     /locus_tag="VW049_000818"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9431975.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16177..16866
                     /locus_tag="VW049_000819"
     CDS             16177..16866
                     /locus_tag="VW049_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9431976.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            16964..17929
                     /gene="cbpC"
                     /locus_tag="VW049_000820"
     CDS             16964..17929
                     /gene="cbpC"
                     /locus_tag="VW049_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9431977.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDPETGIM
                     RTGWQYLGNNWYYLRSSGAMATGWKYVRGKWYYLDNKNGDMKTGWYQDGSTWYYLDPS
                     NGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            17948..18946
                     /gene="cbpJ"
                     /locus_tag="VW049_000821"
     CDS             17948..18946
                     /gene="cbpJ"
                     /locus_tag="VW049_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU9431978.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKELEIKTQDSVGRKYGEKRENSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYIGGERRAGWINDASAWYYLDPATGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNMTVDGYSVNYNGEWVR"
     gene            complement(19064..20296)
                     /locus_tag="VW049_000822"
     CDS             complement(19064..20296)
                     /locus_tag="VW049_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9431979.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEVVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(20307..>20637)
                     /locus_tag="VW049_000823"
     CDS             complement(20307..>20637)
                     /locus_tag="VW049_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9431980.1"
                     /translation="ILYLCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQT
                     TVYNQNKLLMKKIQQVILACSERDDHGLLMFQGDSGKADLFDFGIGSMGVYWCLLNNK
                     FPFDVQT"
BASE COUNT         6433 a         3736 c         4693 g         5775 t
ORIGIN      
        1 acttcgttat tggacggtta cgatattcat attttttgca aagatgttgt ttgaaaaata
       61 attttcaaaa attctgaaaa ttctgttgac aactttctga aaagagtcta taatggagag
      121 aaagttttaa aggagaaaat gatgaaaagt tcaaaactac ttgcccttgc gggcgtgaca
      181 ttattggcgg cgactacttt agctgcatgc tctggatcag gttcaagcgc taaaggtgag
      241 aagacattct catacattta tgagacagac cctgataacc tcaactattt gacaactgct
      301 aaggctgcga cagcaaatat taccagtaac gtggttgatg gtttgctaga aaatgatcgc
      361 tacgggaact ttgtgccgtc tatggctgag gattggtctg tatccaagga tggattgact
      421 tacacttata ctatccgtaa ggatgcaaaa tggtatactt ctgaaggtga agaatacgcg
      481 gcagtcaaag ctcaagactt tgtaacagga ctaaaatatg ctgctgataa aaaatcagat
      541 gctctttacc ttgttcaaga atcaatcaaa gggttggatg cctatgtaaa aggggaaatc
      601 aaagatttct cacaagtagg aattaaggct ctggatgaac agacagttca gtacactttg
      661 aacaaaccag aaagcttctg gaattctaag acaaccatgg gtgtgcttgc gccagttaat
      721 gaagagtttt tgaattcaaa aggggatgat tttgccaaag ctacggatcc aagtagtctc
      781 ttgtataacg gtccttattt gttgaaatcc attgtgacca aatcttctgt tgaatttgcg
      841 aaaaatccga actactggga taaggacaat gtgcatattg acaaagttaa attgtcattc
      901 tgggatggtc aagataccag caaacctgca gaaaacttta aagatggtag ccttacagca
      961 gctcgtctct atccaacaag tgcaagtttc gcagagcttg agaagagtat gaaggacaat
     1021 attgtctata ctcaacaaga ctctattacg tatctagttg gtacaaatat tgaccgtcag
     1081 tcctataaat acacatctaa gaccagcgac gaacaaaagg catcgactaa aaaggctctc
     1141 ttaaacaagg atttccgtca ggctattgcc tttggttttg atcgtacagc ctatgcctct
     1201 cagttgaatg gacaaactgg agcaagcaaa atcttgcgta atctctttgt gccaccaaca
     1261 tttgttcaag cagatggtaa aaactttggc gatatggtca aagagaaatt ggtcacttat
     1321 ggggatgaat ggaaggatgt taatcttgca gattctcagg atggtcttta caatccagaa
     1381 aaagccaagg ctgaatttgc taaagctaaa tcagccttac aagcagaagg tgtgacattc
     1441 ccaattcatt tggatatgcc agttgaccaa acagcaacta caaaagttca gcgcgtccaa
     1501 tctatgaaac aatccttgga agcaacttta ggagctgata atgtcattat tgatatccaa
     1561 caactacaaa aagacgaagt aaacaatatt acatattttg ctgaaaatgc tgctggcgaa
     1621 gactgggatt tatcagataa tgtcggttgg ggtccagact ttgccgatcc atcaacctac
     1681 cttgatatca tcaaaccatc tgtaggagaa agtactaaaa catatttagg gtttgactca
     1741 ggggaagata atgtagctgc taaaaaagta ggtctatatg actacgaaaa attggttact
     1801 gaggctggtg atgaggctac agatgttgct aaacgctatg ataaatacgc tgcagcccaa
     1861 gcttggttga cagatagtgc tttgattatt ccaactacat ctcgtacagg gcgtccaatc
     1921 ttgtctaaga tggtaccatt tacaatacca tttgcattgt caggaaataa aggtacaagt
     1981 gaaccaatct tatataaata cttggaactt caagacaagg cagtcactgt agatgaatac
     2041 caaaaagctc aggaaaaatg gatgaaagaa aaagaagagt ttaataaaaa ggctcaagaa
     2101 gatctcgcaa aacatgtgaa ataactgttg caaaatataa gaaaggattt agtatttctc
     2161 ttgaatgctg aatccttttt tacatttgta aagaaagatt ctaaatgtac tgacccccaa
     2221 aagttggagc ctctttttgt cagaatagag aaaatttttg ttaattttac ttgtttccta
     2281 ttgctttctc agctattatt tgttatatta aaagcataat tattttttat ttatcagggt
     2341 taagcattgc actttcagag gaaggagtat tttttaaaaa gaaaatgtaa acgcttgctc
     2401 aaaaatgaaa ggatttagaa gtttatgaat aaaggattat ttgaaaaacg ttgtaaatat
     2461 agtattcgga aattttcatt aggtgttgct tctgttatga ttggagctgc attctttggg
     2521 acaagtccgg ttcttgcaga tagcgtgcag tctggttcca cggcgaactt accagctgat
     2581 ttagctactg ctcttgcaac agcaaaagag aatgatgggc gtgattttga agcgcctaag
     2641 gtgggagaag accaaggttc tccagaagtt acagatggac ctaagacaga agaagaacta
     2701 ttagcacttg aaaaagaaaa accggctgaa gaaaaaccaa aagaggataa acctgtagct
     2761 gctaaacctg aaacacctaa gacggtaacc cctgaatggc aaacggtaga gaaaaaagaa
     2821 caacagggaa cagtcgctat ccgagaagaa aaaggtgtcc gctacaacca actatcctca
     2881 actgctcaaa atgataacgc aggcaaacca gccctgtttg aaaagaaggg cttgaccgtt
     2941 gatgccaatg gaaatgcaac tgttgattta accttcaaag aagattctga aaagggcaaa
     3001 tcacgctttg gtgtcttctt gaaatttaaa gataccaata ataatgtttt tgtcggttat
     3061 gacaaggatg gctggttctg ggagtataaa tctccaacaa ctagcacttg gtatagaggt
     3121 agtcgtgttg ctgctcctga aacaggatca acaaaccgtc tctctatcac tctcaagtca
     3181 gacggtcagc taaatgccag caataacgat gtcaatctct ttgacacagt gactctacca
     3241 gctgcggtca atgaccatct taaaaatgag aagaagattc ttctcaaggc gggctcttat
     3301 gacgatgagc gaacagttgt tagcgttaaa acggataacc aagagggggt aaaaacagag
     3361 gatacccctg ctgaaaaaga aacaggtccg gttgttgacg atagcaaggt gacttatgac
     3421 acgattcagt ctaaggtcct caaagcagtg attgaccaag ccttccctcg tgtcaaggaa
     3481 tacagcttga atggacatac tttgccagga caggtgcaac agttcaacca agtctttatc
     3541 aataaccacc gaatcacccc tgaagtcact tataagaaaa tcaatgagac aacagcagag
     3601 tacttgatga agcttcgcga tgatgctcac ttaatcaatg cggaaatgac agtacgcttg
     3661 caagttgtgg acaatcaatt gcactttgat gtgaccaaga ttgtcaacca caatcaagtc
     3721 actccaggtc aaaagattga tgacgaaaga aaactacttt cttctattag tttcctcggc
     3781 aatgctttag tctctgtttc tagtgatcaa actggtgcta agtttgatgg ggcaactatg
     3841 tcaaacaata cgcatgtcag tggagatgat catatcgatg taaccaatcc aatgaaagac
     3901 ctagccaagg gttacatgta tggatttgtt tctacagata agcttgctgc tggtgtttgg
     3961 agtaactctc aaaacagcta tggtggtggt tcgaatgact ggactcgttt gacagcttat
     4021 aaagaaacag tcggaaatgc caactatgta ggaatccaca gctctgaatg gcaatgggaa
     4081 aaagcttata agggcattgt tttcccagaa tacacgaagg aacttccgag tgctaaggtt
     4141 gttatcactg aagatgccaa tgcagacaag aaagttgatt ggcaagatgg tgccattgct
     4201 tatcgtagca ttatgaacaa ccctcaaggt tgggaaaaag ttaaggatat cacagcttac
     4261 cgtatcgcga tgaactttgg ttctcaagca caaaatccat tccttatgac cttggatggt
     4321 atcaagaaaa tcaatctcca cacagatggt cttgggcaag gtgttctcct taaaggatat
     4381 ggtagcgaag gccatgactc tggtcacttg aactatgctg atattggtaa gcgtatcggt
     4441 ggtgtcgaag acttcaagac tctaattgag aaggctaaga aatatggagc tcatctaggt
     4501 atccacgtta acgcttcaga aacttatcct gagtctaaat acttcaatga aaaaattctc
     4561 cgtaagaatc cagatggaag ctatagctat ggttggaact ggctagatca aggtatcaac
     4621 attgatgctg cctatgacct agctcatggt cgtttggcac gttgggaaga tttgaagaaa
     4681 aaacttggtg acggtctcga ctttatctat gtggacgttt ggggtaatgg tcaatcaggt
     4741 gataacggtg cctgggctac ccacgttctt gctaaagaaa ttaacaaaca aggctggcgc
     4801 tttgcgatcg agtggggcca tggtggtgag tacgactcta ccttccatca ctgggcagct
     4861 gacttgacct acggtggcta caccaataaa ggtatcaaca gtgccatcac ccgctttatc
     4921 cgtaaccacc aaaaagatgc ttgggttggg gactacagaa gttatggtgg tgcagccaac
     4981 tatccactgc taggtggcta cagcatgaaa gactttgaag gctggcaagg aagaagtgac
     5041 tacaatggct acgtgactaa cctctttgct catgacgtca tgactaagta cttccaacac
     5101 ttcactgtaa gtaaatggga aaatggtaca ccggtgacta tgaccgataa cggtagcacc
     5161 tataaatgga ctccagaaat gcgagtggag ttggtagatg ctgacaataa taaagtagtt
     5221 gtaactcgta agtcaaatga tgtcaatagc ccacaatatc gcgaacgtac agtaacgctc
     5281 aacggacgtg tcatacaaga tggttcagct tacttgactc cttggaactg ggatgcaaat
     5341 ggtaagaaac tttctactga gaaggaaaag atgtactact tcaatacgca ggccggtaca
     5401 acaacttgga cccttccaag cgattgggca aagagcaagg tttaccttta caagctaact
     5461 gaccaaggta agacagaaga gcaagaacta actgtaaaag atggtaaaat taccctagat
     5521 cttctagcaa atcaaccata cgttctctat cgttcgaaac aaaccaatcc tgaaatgtca
     5581 tggagtgaag gcatgcacat ctatgaccaa ggatttaata gcggtacctt gaaacattgg
     5641 accatttcag gcgatgcttc taaggcagaa attgtcaagt ctcaaggggc aaacgatatg
     5701 cttcgtattc aaggaaacaa agaaaaagtt agtctcactc agaaattaac tggcttgaaa
     5761 ccaaatacca agtatgccgt ttatgtcggt gtcgataacc gtagtaatgc caaggcgagc
     5821 atcactgtaa acactggtga aaaagaagtg actacttata ccaataagtc tctcgccctc
     5881 aactatgtta aggcctacgc ccacaataca cgtcgtaaca atgctacagt tgacgataca
     5941 agttacttcc aaaacatgta cgccttcttt acaactggat cggacgtatc aaatgttact
     6001 ctgacattga gtcgtgaagc tggtgatgaa gcaacttact ttgatgaaat tcgtaccttt
     6061 gaaaacaatt caagcatgta cggagacaag catgatacag gtaaaggcac cttcaagcaa
     6121 gactttgaaa atgttgctca gggtatcttc ccatttgtag tgggtggtgt cgaaggtgtc
     6181 gaagacaacc gcactcactt gtctgaaaaa cacgatccat atacacaacg tggttggaat
     6241 ggtaagaaag tcgatgatgt tatcgaagga aattggtcac tcaagacaaa tggactagtg
     6301 agtcgtcgta acttggttta ccaaaccatc ccacaaaact tccgctttga agctggtaag
     6361 acctaccgtg taacctttga atacgaagca ggttcagaca atacctatgc ttttgtagtc
     6421 ggtaagggag aattccaatc aggtcgtcgt ggtactcaag caagcaactt ggaaatgcat
     6481 gaattgccaa atacttggat agattctaag aaagccaaga aggtaacctt ccttgtgaca
     6541 ggtgcagaaa caggggatac ttgggtaggt atctactcaa ctggcaatgc aagtaatact
     6601 cgtagtgatt ctggtggaaa tgccaacttc cgtggttata acgacttcat gatggataat
     6661 cttcaaatcg aagaaattac cctaacaggt aagatgttga cagaaaatgc tctgaagaac
     6721 tacttgccaa cggttgccat gactaactac accaaagagt ctatggatgc tttgaaagag
     6781 gcggtcttta acctcagtca ggccgatgat gatatcagtg tggaagaagc gcgtgcagag
     6841 attgccaaga ttgaagcctt gaagaatgct ttggttcaga agaaaacggc tttggtagca
     6901 gatgactttg caagtcttac agctcctgct caggctcaag aaggtcttgc aaatgccttt
     6961 gatggaaact tatctagttt atggcataca tcatggggcg gaggagatgt aggcaagcct
     7021 gcaaccatgg tcttgaaaga accaactgaa ataactggac ttcgttatgt tccacgtgga
     7081 tcaggttcaa atggtaactt gcgtgatgtg aaacttgttg tgacagatga gtctggcaag
     7141 gagcatacct ttactgcaac tgattggcca gataacaata agtcaaaaga tattgacttt
     7201 ggtaagacaa tcaaggctaa gaaaattgtc cttactggta ccaagactta cggagatggt
     7261 ggcgataaat accaatctgc agcggaactc atctttactc gtccacaggt agcagaaaca
     7321 cctcttgact tgtcaggcta tgaagcagct ttggctaagg ctcagaaatt aacagacaaa
     7381 gacaatcaag aggaagtagc tagcgttcag gcaagcatga aatatgcgac ggataaccat
     7441 ctcttgacgg aaagaatggt ggaatacttt gcagattatc tcaaccaatt aaaagattct
     7501 gctacgaaac cagatgctcc aactgtagag aaacctgagt ttaaacttag ctctttagct
     7561 tccgatcaag gtaagacgcc agattataag caagaaatag ctagaccaga aacacctgaa
     7621 caaatcttgc cagcaacagg tgagagtcaa tctgacacag ccctcttcct agcaggtgtt
     7681 agcctagccc tatctgctct ctttgtagta aaaacgaaga aagactagta tttagtaaaa
     7741 cctcttaaca agattacgga agcagtctct atcttttcca atgaggttta tagtacagaa
     7801 aaagcctgag aagatgtctt ctcaggcttt tgttaagcac ataaatacaa tagtgctatg
     7861 acaaaatcac ctagaaaaat ctgggtgata aatgttatgg ttgtgctggt tgaggattct
     7921 gattttgttg atcaggggtt gtatttgatt gttgcgtatt attgttagga ttggtagtcg
     7981 tactattatt tgtgcttgga gtggttgagc tagactgtga agttgaacta tctgatgatg
     8041 agcttgaact ttcagttgat gggggttgtt gtggagcagg tgagttccac gtagaacgag
     8101 caccattttt aaatacgaat tctccatttc tgtagagccc ctctggtata ttccaatctt
     8161 ctggattgct tccttcagac aggtaggtca tcatagagcg gtaaactttg gcagcgaccg
     8221 taaggccatt gcctacaagt ggtgtcagac ggttagaata gcctgtccat acagccattg
     8281 aatatttacg cgtatagcca gcaaatagtt catcaggtgc tacaaattga gaggtcttga
     8341 tgtggttttc aatttcctcg tctgtatagt tagaggttcc tgttttacca gcctgaggaa
     8401 gccaagcaag ataggcattt cgtccagttc cataagtcaa gactgttttc atcatgtcgg
     8461 tcatcatata ggctgtcgtt tctttcatgg cacgagttcc gacattagag aactcttttt
     8521 cactcccatc actaaagacg actttatgga tatacattgg tttatagtaa gttccaccat
     8581 ttgcaaaggc agcgtaagca gcagccatct tttcactact tgctccatat tttttgtctg
     8641 attcggttgt gttacttgaa atggcatttg agtagtgaat acttgggtag tcgattccga
     8701 gaccatttag gaaagtcttg gcgcggttga gtccgacctt gtttagagtt tccacggctg
     8761 ggacgtttcg cgattgttgc agggcgtatt gcaaggtgat gttgccaaag tagcccctat
     8821 cccagttata aacaggagta tttgtcccag ggtaattata gggctcatcg tgaacgatag
     8881 tagcagttga atcgtagaca ccgtactcca aggcaggagc atagtctgtg atcggtttca
     8941 tagttgatcc ccagtcgcgg tttgtttcta ctgcttggtt aattccgaag gaaacattac
     9001 ttgactgatg gcgtgctcct agctgggcaa tgactttacc gttagaaaca tcaacaatgg
     9061 tagaagcgac ttgcaattca tcgtctggat aggcaacgta ttcgtctgta ttgtaaatat
     9121 cccacagatg tttttgagct tcttggtcta catttgtgta gacatccatc ccagttgtga
     9181 gcaggttata gcctgtttct tcttcaactt gattgatgac ttccttgagg taattatcca
     9241 tgtaagcagg gtaattactt gctgatttga gactttgtag tccatcagta attggtgtat
     9301 tgactgcttt ctcatactgt tcagcagaga tgtagccttg atttttcatt tcagataaga
     9361 ccaagtttcg gcggtcttgg gctgcttctg gatgtgaata ggggtcatat tggtttggtg
     9421 cctgaggcat tccagccagc aaggctaact gaggtaaact taaattattg aggtctttac
     9481 catagtagtt ttgagctgct gtctgcattc catagttccc attagacatg tagaccttat
     9541 ttatatagta ggtcaagatt tcttgcttgg ttgctttttg ttctaactga atcgctaacc
     9601 aagcttcctg agccttacga gaaatagtct ggtcggaagt cgaagttgaa aagtaagtca
     9661 acttaatcaa ctgttgggtg agagttgatc caccttggag ggaattgctt tgcagattgc
     9721 gcaagaaagc tcccaggata cggatggtat caatccccct gtggtcgaag aagcgatggt
     9781 cttcgataga aacgattgcc ttaaccaaat ctgtgggaat atcattagct tgggcattga
     9841 cgcggcgttc agaacccaag tcagcaatga gttgattttt attgtcgtag attttactag
     9901 aagttgttgc aactagttta ctctcggata ggctaggagc cttgctaacg tagtagaaaa
     9961 aaactcctcc gcctaagaca atggctgcga taaccaagct taagaagcta atgctcagat
    10021 acttgattag gcgcagaatc gttggtttgt tcatcttgtt ttaccaccta ataaatgttc
    10081 tttgataaca ttgagataag gaatttgagg gaaggcacca gccttgattt catatccata
    10141 ttctcgaata tattcaagtg gcattgattt ttgtccctta tcttgatgat agaagcgaat
    10201 caaatcgaat gccggcaata agtaggtttc ttgctgagaa gaaaagtgaa gaaggacaaa
    10261 gcagattcct tgttgggcaa ggacttgttc catatgctga atctgatgtg gatgaaaatt
    10321 tttcatcgga atcgcacgtt tttgttttgt ttccttgact tcaaagtcga tgtaatatcc
    10381 attataaacg ccagaatagt ccgtcgttga agcttgtcga aaataggctt caacaatctt
    10441 ggcacgactt cgttgtggat agtccacttg tacgatttga ataggagttg gtttcttatg
    10501 tataacagcc aagccctgag acaaatagta gtcgttggta gcattgatca tcttttcaaa
    10561 agacattcct cgatttgcga aatttttggg ttgagaaaga gatgtttgtc ttttttgtga
    10621 tgaaacttta tgtggatagt tgaccataat tctccttatt ggtacaataa catcacttta
    10681 ttataccata atttgcacag aaagggttaa aaatggctac agctttggtt ttaggctatt
    10741 ctgcttttga tttgggttta ttttctgata aggatcctcg tcttaaattg attaaaaaag
    10801 cgatacgtaa agatttagag gctatggcag cagatggggt gtcttggctt gtgtttacag
    10861 gaagtttggg ttttgaatat tgggttttag aggttgctca ggagatgaaa accgaatacg
    10921 gtttccagtt ggccaccatt tttgcttttg aaacccatgg ggaaaattgg aatgaaggca
    10981 atcagatgaa actgagtcgc tttaagcagg tagactttgt caaatatgct tatcctcgct
    11041 atgaacacaa ggggcagtta agagattacc agcagttttt gctggaaaac acgactagtt
    11101 cctatctttt ttatgatgaa gaaaatgaaa caaaactagc ttatttttac caaaagatga
    11161 aaaatcaaga ggactatttt ataaagagat taacatttga tcaattaaat gaacttgctg
    11221 aaaatttttc cgaaaattga agctttgacc ttgatttttg tttgcctttt tttatataat
    11281 aatactagca agcgagaatg gagagagaca tggcaagtat tattttttca gcgaaagata
    11341 tttttgaaca agagtttgga cgtgaagtcc gtggctataa taaagtagaa gttgacgagt
    11401 ttttagacga tgtcatcaag gactatgaaa cctatgctgc cttggtcaag tcacttcgtc
    11461 aggaaattgc ggatttgaag gaagaattaa ctcgtaaacc gaaaccttca ccagttcaag
    11521 cagaacccct tgaagcggca attacaagtt ctatgacgaa ttttgatatt ttgaaacgcc
    11581 tgaatagatt ggaaaaagaa gtttttggta aacaaatttt agataactca gatttttaag
    11641 tagttatttg agatgtgcaa tttttggata atcgcgtgag gagaattgct tctcatgagg
    11701 aaagtccatg ctagcacagg ctgtgatgcc tgtagtgttt gtgctaggcg aaaccataag
    11761 cctagggacg aggaatcgtt acggcagttg aaatggctaa gtccttggat aggccagagt
    11821 aggcttgaaa gtgccacagt gacgaagtct ttctggaaac agagagagtg gaacgcggta
    11881 aacccctcaa gctagcaacc caaattttgg tcggggcatg gagtacgcgg aaacgaacgt
    11941 agtattctga ctgctatcag ctagagctgt tagtggtaga cagatgatta tcgaaggaag
    12001 tggtcctagt cacttctgga acaaaacatg gcttatagaa aattgcatat aggttggggc
    12061 tgagaaattt tctcaacctc attttttaaa gtggacatat agaaaggtct tgcaagactg
    12121 taacatgaaa aaagaattta atttaattgc aactgcggca gcagggcttg aggctgtcgt
    12181 tggtcgtgaa gtgcgagagt tgggctacga ttgtcagatt gaaaatggac gtgttcgttt
    12241 tcaaggagac gtgagagcta ttatcgaaac caacctttgg ctgcgggcag cagatcgtat
    12301 caaaattatc gtaggaacgt tccctgctaa gacttttgaa gagctatttc agggagtttt
    12361 cgctttagat tgggaaaatt atttaccact tggagctcgg ttcccgattt caaaagctaa
    12421 atgtgttaag tccaaacttc acaatgagcc cagtgttcag gctatttcta agaaagctgt
    12481 tgtcaagaaa ttgcagaaac actatgctcg cccagaaggg gttcctctga tggagaatgg
    12541 cccagagttt aagattgagg tctctattct caaagatgtg gcaactgtca tgattgatac
    12601 gaccgggtct agcctcttta aacgtggtta tcgtaccgaa aaaggtggcg ctcctatcaa
    12661 ggaaaatatg gcagcagcca ttttacaact ttctaactgg tatccagaca agcctttgat
    12721 tgatccgacc tgtggttcgg ggactttctg tattgaggca gttatgattg ctagaaagat
    12781 ggcgccaggt cttcgtcgct cttttgcatt tgaggaatgg aactggatca gcgatcgctt
    12841 gattcaagaa gtgcgcacag aagcggctaa aaaagtagac cgtgagcttg agctggatat
    12901 catgggctgt gatattgatg ctcgcatggt ggaaattgct aaggccaatg ctcaggcagc
    12961 tggtgttgca ggagacatta cttttaagca gatgcgcgtg caggatttac gttccgataa
    13021 aatcaatgga gtaatcattt ccaatccgcc ttatggtgaa cgtttgtcag atgatgcagg
    13081 ggtgaccaag ctctatgctg agatggggca agtatttgca ccgctgaaaa cttggagcaa
    13141 atttatcctg actagtgatg aagcttttga aagcaagtat ggtagccaag cagataagaa
    13201 gcgtaagtta tacaacggaa ccttgaaagt ggatctgtat caatattttg gtcagcgtgt
    13261 caaacggcaa gaggtaaaat agaaagggat actcatgagt aaaaaaagac gaaatcgtca
    13321 aaaaaaagaa ggtcaagaac cgcaatttga ttttgatgaa gcaaaagagc taacagttgg
    13381 tcaagctatt cgtaaaaatg aagaagtgga agcaggagtc ttgcctgagg attccatttt
    13441 ggacaagtat gttaagcaac acagagatga aattgaggcg gataagtttg cgactcgtca
    13501 atacaaaaaa gaggagttcg ttgaaactca gagtctggat gatttaattc aagagatgcg
    13561 tgaggctgta gagaagtcag aagcttcttc ggaggaagtt ccatcttctg aagacatctt
    13621 actacccttg cctctggacg atgaggagca aggcttggat cctctattgc tagatgatga
    13681 aaatccaaca gaaatgactg aagaagtgga agaggagcaa aacctttctc gtctggatca
    13741 agaggactca gaaaagaaaa gtaaaaaagg ctttattttg accgttttgg cgcttgtatc
    13801 agtaattatt tgtgtcagtg cttattatgt ctaccgtcaa gtggctcgtt cgaccaagga
    13861 aattgaaact tctcaatcaa ctacagccaa tcaatcggat gtggatgatt ttaatacact
    13921 ttatgacgcc ttttacacag atagcaataa aacggctttg aaaaatagcc agtttgataa
    13981 actgaatcaa ctcaagactt tacttgataa gctggaaggt agtcgtgaac atacgcttgc
    14041 caaatctaaa tatgatagtc tagtaacgca aatcaaggct attcaagatg tcaatgctca
    14101 atttgagaaa ccagctattg tggatggtgt gttggatgcc aatgccaaag ccaaatcgga
    14161 tgctaaattt acggatatta aaactggaaa tacggagctt gataaagtgc tagataaggc
    14221 tatcagtctt ggtaagagcc agcaaacaag tacttctagc tcaagttcaa gtcaagcaag
    14281 ctcaaatacg acaagtgagc caaaaccaag tagttcaaat gagactagaa gtagtcgcag
    14341 tgaagtcaat atgggtctct cgagtgaagg ggttgctgtt caaagaagtg ccagtcgtgt
    14401 tgcctataat cagtctgcta ttgatgatag taataactct gcctgggatt ttgcggatgg
    14461 tgtcttggaa caaattctag cgacttcacg ttcacgtggc tatatcactg gagaccaata
    14521 tatccttgaa cgtgtcaata tcgttaacgg caatggttat tacaacctct ataagccaga
    14581 tggaacctat ctctttaccc ttaactgtaa gacaggctac tttgtcggaa atggcgctgg
    14641 tcatgcagat gacttggact actaagcagt cgttacaaaa ttctttcttt tcaaaagtaa
    14701 aaatgataaa ataaaacaaa tgaaacaaga ggagtgtcaa atgacaaaag ctaactttgg
    14761 tgtcgtaggt atggccgtaa tgggtcgtaa ccttgccctt aatattgaat cacgtggtta
    14821 cacagttgct atctacaacc gtagtaaaga aaaaacggaa gatgtgattg cttgccatcc
    14881 tgaaaagaat tttgtaccaa gctatgacgt tgaaagtttt gtaaactcaa tcgaaaaacc
    14941 tcgtcgtatc atgctgatgg ttcaagctgg acctggtaca gatgctacta tccaagccct
    15001 tcttccacac cttgacaagg gtgatatctt gattgacgga ggaaacactt tctacaaaga
    15061 taccatccgt cgtaatgaag aattggcaaa ctcaggtatc aactttatcg gtactggagt
    15121 ttctggtggt gaaaaaggtg cccttgaagg tccttctatc atgcctggtg gacaaaaaga
    15181 ggcctacgaa ttggtttcgg atgttcttga agaaatctca gctaaagcac cagaagatgg
    15241 caaaccatgt gtgacttata tcggtcctga tggagctggt cactatgtga aaatggttca
    15301 caacggtatc gagtacggtg atatgcaatt gatcgcagaa agctatgact tgatgcaaca
    15361 cttgctaggc ctttctgcag aggatatggc tgaaatcttt actgagtgga acaagggtga
    15421 attggatagc tacttgatcg aaatcacagc tgatatcttg agccgtaaag acgatgaaga
    15481 ccaagatgga ccaatcgtag actacatcct tgatgctgca ggtaacaagg gaactggtaa
    15541 atggacgagc caatcatctc ttgaccttgg tgtaccattg tcactgatta ctgagtcagt
    15601 gtttgcacgc tacatttcaa cttacaaaga agaacgtgta catgctagca aggtgcttcc
    15661 aaaaccagct gccttcaact ttgaaggaga caaggctgaa ttgattgaaa aaatccgtca
    15721 agccctttac ttctcaaaaa tcatttcata cgcacaagga tttgctcaat tgcgtgtagc
    15781 ctctaaagaa aacaactgga acttgccatt tgcagatatc gcatctatct ggcgtgatgg
    15841 ctgtatcatc cgttctcgtt tcttgcaaaa gattacagat gcttacaacc gcgatgcaga
    15901 tcttgccaac cttcttttgg acgagtactt cttggatgtt actgctaagt atcaacaagc
    15961 agtgcgtgat atcgtagctc ttgcggttca agctggtgtg ccagtgccaa ctttctcagc
    16021 agctattact tactttgata gctaccgttc agctgacctt ccggctaact tgatccaagc
    16081 acaacgtgac tactttggtg ctcacactta ccaacgtaaa gacaaagaag gaaccttcca
    16141 ctactcttgg tatgacgaaa aataagtagg tcagccatgg ggaaacggat tttattactt
    16201 gagaaagaac gaaatctagc tcatttttta agtttggaac tccagaaaga gcagtatcgg
    16261 gttgatctgg tagaggaggg gcaaaaagcc ctttccatgg ctcttcagac agactatgat
    16321 ttgattttat tgaacgttaa tctgggagat atgatggctc aggattttgc agaaaaattg
    16381 agtcgaacta aacctgcctc agtcatcatg attttagatc attgggaaga cttgcaagaa
    16441 gagctggaag ttgttcagcg ttttgcagtt tcatacatct ataagccagt ctttatcgaa
    16501 aatctggtag cgcgtatttc ggcgatcttc cgaggtcggg acttcattga tcaacactgc
    16561 agtctgatga aagttccaag gacctaccgc aatcttagga tagatgttga acatcacacg
    16621 gtttatcgtg gtgaagagat gattgctctg acccgccgtg agtatgacct tttggcgaca
    16681 cttatgggaa gcaagaaagt attgactcgt gagcaactgt tggaaagtgt ttggaagtat
    16741 gaaagtgcga ccgagacaaa tatcgtagat gtctatatcc gctatctacg gagcaagctt
    16801 gatgttaaag gtcaaaaaag ctacattaaa accgtgcgtg gtgttggtta caccatgcaa
    16861 gaatagaaaa gcagttgcag ttgtgtaact gcttttttga ggagttttta tatattgaca
    16921 tgcagtcggg tctttgctac aatcgtttta ggaggatata tagatgaaac ttttgaaaaa
    16981 aatgatgcaa gttctactag cagtcttttt ctttggtttg ctagctacaa atacggtatt
    17041 tgcgaatacc acaggtggcc gatttgttga taaggataat agaaaatatt atgtaaaaga
    17101 tgatcataaa gcaatctatt ggcataaaat agacggtaaa acttactatt ttggtgatat
    17161 tggagagatg gttgtcggtt ggcaatactt agaaattcct ggaacaggtt atcgtgataa
    17221 tttattcgat aaccaaccag ttaatgaaat tggccttcag gagaagtggt actattttgg
    17281 acaagatggt gctttgctag aacaaacaga taaacaagta ctagaggcaa aaacgtctga
    17341 aaatacagga aaagtatacg gtgaacaata tcctctatct gctgaaaaga gaacttatta
    17401 ttttgataat aattatgctg taaagacagg ctggatttat gaagacggca attggtatta
    17461 tttaaataag ctaggaaatt ttggcgatga ttcttacaat ccactaccaa ttggtgaagt
    17521 tgctaagggt tggactcaag attttcatgt tactattgac attgatagaa gcaaacctgc
    17581 tccatggtac tacctagacc cagaaactgg cattatgcga acaggatggc aatatctagg
    17641 taataactgg tactacctcc gttcatcagg agcaatggca acaggttgga aatatgtacg
    17701 aggcaaatgg tattacttag ataataaaaa tggtgatatg aagactggtt ggtatcaaga
    17761 tggttcaact tggtactatt tagatccttc taatggagat atgaaaatag gttggacaaa
    17821 agtaaatgga aaatggtatt atctcaattc aaatggagca atggttacag gtagtcaaac
    17881 tatcgatggt aaagtttata acttcgcttc atctggtgag tggatttaat gttggaggat
    17941 atataaaatg aagcttttga aaaaaactat gcaagctgga ctaacagtaa ttttctttgg
    18001 tttgctagct acgaatacag tatttgcaga taactctgaa ggatggcagt ttgtccaaga
    18061 aaacggtaga acctattaca aaaaggggga cctcaaagaa acctactggc gagtgattga
    18121 tggtaagtac tattattttg attctctatc tggagagatg gttgtcggct ggcaatatat
    18181 cccgtttcca tctaaaggta gtacaattgg tccttaccca aatggtatca gattagaagg
    18241 ttttccaaag tcagagtggt actactttga taaaaatgga gtgctacaag agtttgttgg
    18301 ttggaaagaa ttagagatta aaactcaaga cagtgttgga agaaagtatg gggaaaaacg
    18361 tgaaaattca gaagataaag aagagaagcg ttattacacg aactattact ttaatcaaaa
    18421 tcattcttta gagacaggtt ggctttatga tcagtctaac tggtattatc tagctaaaac
    18481 agaaattaat ggagaaaact atattggtgg tgaaagacgt gcaggttgga taaatgatgc
    18541 ttcagcttgg tactacctag atccagcaac tggtattatg caaacaggtt ggcaatatct
    18601 aggtaataag tggtactacc tccgttcctc aggagcaatg gccactggct ggtatcagga
    18661 aggtaccact tggtattatt tagaccaccc aaatggcgat atgaaaacag gttggcaaaa
    18721 ccttgggaac aaatggtact atctccgttc ctcaggagct atggcaactg gttggtatca
    18781 agatggttca acttggtact acctaaatgc aggtaatgga gacatgaaga caggttggtt
    18841 ccaggtcaat ggcaactggt actatgctta tagctcaggt gctttggcag tgaatatgac
    18901 cgtagatggc tattctgtca actataatgg cgaatgggtt cggtaatgac agggcgctta
    18961 taattatatt agaacactcg cttaatcaaa gaagtgactt tgttgtactt gacctgacga
    19021 ttggggagat taaaagcatg tgaaatctac acatgctttt tcgttagtta tgagtgttga
    19081 acttgactaa ccattgacaa aagataagag caatcaaaat aagatacaaa aatatcaatg
    19141 ctaactgcac cgaaacgccc gatgctatgg atagggctaa tagggagagc agggacgggg
    19201 cgagaatgtc tactgtgctc agagcagagc ccaccgtccc catcttgtca ctggggatat
    19261 gggcaaagac agctgcttgt aggcgagggc tgagaatgcc gataatcgta gaactgatga
    19321 aactggtcac gaaaattacg atataggctt gacaaaggat actagttatt agagccaata
    19381 gggaaatctc acagaaaacc aagaggtgcg tgaaacggat atttttaaat agattactgc
    19441 tggtcatatt gccaaggata gctccacaag acatgatgat ggagagcagg gcgaagagtt
    19501 gcccaatgct cagttgtgct agtgtctgtt ggatgaagcg gagggcaatc agccgagggg
    19561 acacatccaa gatggcaatc atactggtcg tcagaaacag tagtatgacg gtatctttta
    19621 acctcaggag cacagccagc gactgtttaa gatgttgaaa atatttctta aaactcagtg
    19681 ctgtatgtga catttcaatc cttttttcaa cctcatacaa ggtatgtcga ataacataca
    19741 ggcccaaaaa ggcaatgaca aaagtcagag tgttgataag ggaaatagtt tgaatactca
    19801 taacattgat aaggaatgcg ccagctagat tggcgacaat gcggacaacc ctcatcagag
    19861 actgcctgaa ccccatggca tcatgcaggt cgtccttaat tacactgatg tagagggcgt
    19921 tcatcatgta ggcagacagg tagctcaagg tgtcagagat gaggttgatg atgacaatca
    19981 taatgactga aactaatgaa aagggcgaca agacgagaaa tactgtcaaa atagcgtaga
    20041 gcaggatttt gattttggca atccagagtg cgtgtttgac tctattttct tgaaaatctc
    20101 ctagaattcc gagaaagagt tgcgatagta ggggaaccac ctctgaaatg gaaatcagta
    20161 aaatcgctag cggagcgaaa ttgtactgag ccacataatt gataatggca aggtagaaaa
    20221 tactgtcgcc gaatgatgaa aaacagctgt ttagcaacaa ctgtctatat atgttgtttg
    20281 tccaaaataa tttcatacat ttctccttat gtctgcacat cgaatgggaa tttattattt
    20341 aatagacacc aatatacccc catgcttcct attccgaagt caaacaaatc tgctttacca
    20401 ctatctcctt gaaacatcag taaaccgtga tcatctcgtt cagaacatgc taaaattacc
    20461 tgttggattt ttttcatcag taatttgttt tgattgtaga cagtggtttg tagtaagcta
    20521 gttattccgt ggcaatatcc tgtcatcatt tttagatgat ggttaaaaac aaattcttga
    20581 tatttactaa taatatcttt gtttccgtca caatcgtaca tacaaagata taagatt
//
LOCUS       DAVKGC010000028        20278 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKGC010000028 DAVKGC010000000
VERSION     DAVKGC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.028"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(85..720)
                     /locus_tag="VW049_000824"
     CDS             complement(85..720)
                     /locus_tag="VW049_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9431981.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNSLEAIIQSVARDYPLTEIKLLSILPVNEGEKYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(722..1549)
                     /locus_tag="VW049_000825"
     CDS             complement(722..1549)
                     /locus_tag="VW049_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9431982.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(1571..2497)
                     /locus_tag="VW049_000826"
                     /pseudo
     CDS             complement(1571..2497)
                     /locus_tag="VW049_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            3148..3759
                     /locus_tag="VW049_000827"
     CDS             3148..3759
                     /locus_tag="VW049_000827"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9431983.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            3804..4532
                     /gene="rlmB"
                     /locus_tag="VW049_000828"
     CDS             3804..4532
                     /gene="rlmB"
                     /locus_tag="VW049_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9431984.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(4571..5482)
                     /gene="trxB"
                     /locus_tag="VW049_000829"
     CDS             complement(4571..5482)
                     /gene="trxB"
                     /locus_tag="VW049_000829"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9431985.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(5548..5772)
                     /locus_tag="VW049_000830"
     CDS             complement(5548..5772)
                     /locus_tag="VW049_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9431986.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(5850..6593)
                     /locus_tag="VW049_000831"
     CDS             complement(5850..6593)
                     /locus_tag="VW049_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9431987.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(6593..7393)
                     /locus_tag="VW049_000832"
     CDS             complement(6593..7393)
                     /locus_tag="VW049_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9431988.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(7551..7907)
                     /locus_tag="VW049_000833"
     CDS             complement(7551..7907)
                     /locus_tag="VW049_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9431989.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(7920..8432)
                     /locus_tag="VW049_000834"
     CDS             complement(7920..8432)
                     /locus_tag="VW049_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9431990.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(8432..8926)
                     /locus_tag="VW049_000835"
     CDS             complement(8432..8926)
                     /locus_tag="VW049_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9431991.1"
                     /translation="MEIRLAFPNEVGAIMQVIEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            9061..9507
                     /locus_tag="VW049_000836"
     CDS             9061..9507
                     /locus_tag="VW049_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9431992.1"
                     /translation="MKTTFSYPKWAEIPNINLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            9504..10151
                     /locus_tag="VW049_000837"
     CDS             9504..10151
                     /locus_tag="VW049_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9431993.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(10172..10753)
                     /gene="pdxT"
                     /locus_tag="VW049_000838"
     CDS             complement(10172..10753)
                     /gene="pdxT"
                     /locus_tag="VW049_000838"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9431994.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(10754..11629)
                     /gene="pdxS"
                     /locus_tag="VW049_000839"
     CDS             complement(10754..11629)
                     /gene="pdxS"
                     /locus_tag="VW049_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9431995.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(11781..13160)
                     /locus_tag="VW049_000840"
     CDS             complement(11781..13160)
                     /locus_tag="VW049_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9431996.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            13454..14377
                     /locus_tag="VW049_000841"
     CDS             13454..14377
                     /locus_tag="VW049_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9431997.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            14437..15042
                     /locus_tag="VW049_000842"
     CDS             14437..15042
                     /locus_tag="VW049_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9431998.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            15061..16305
                     /locus_tag="VW049_000843"
     CDS             15061..16305
                     /locus_tag="VW049_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9431999.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRHFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(16422..16679)
                     /locus_tag="VW049_000844"
     CDS             complement(16422..16679)
                     /locus_tag="VW049_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9432000.1"
                     /translation="MDPKKIARINELAKKKKTEGLTLEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(16721..18757)
                     /locus_tag="VW049_000845"
     CDS             complement(16721..18757)
                     /locus_tag="VW049_000845"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9432001.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGGATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(18969..19886)
                     /gene="glyQ"
                     /locus_tag="VW049_000846"
     CDS             complement(18969..19886)
                     /gene="glyQ"
                     /locus_tag="VW049_000846"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9432002.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
BASE COUNT         5868 a         4631 c         3703 g         6076 t
ORIGIN      
        1 taaattcctc cgtcactttt tattttaaag gattttagaa ttttttacaa gatattatgc
       61 tcaaatttaa cattatttag ctatttagta aagatagtct ttcaaggctt ttgacaaagc
      121 ctgataacca gcaatactga ggtgcagtcc atcagttgta tattcttttt tgagttggcc
      181 tgcttggtct gtcaaacaat caaatactgg cacaaattcc acctgcatat aggcagatgc
      241 aagctcttga taggcttgat tccaattctg aattttttca ttcgagcgga tatagactgc
      301 ctgctggtac ttctctccct cattgacagg caaaatggaa agcaatttaa tctctgtcaa
      361 tggataatcg cgagcaacgg attgaatgat agcttcgaga ctattgagag cctcattcac
      421 aggaacatcc tttccgatat catttgtccc aatcagaaga aaaattttat ctactgctcc
      481 accatataga tgagcatcaa ggttctctag taacagtcct gtctgatagc cacgaattcc
      541 tcgattgaca atcgtctttg aagtcccaaa tagctcctgt agaggataat actcgacaat
      601 ggaatcccca ataaaaagaa tgtttggttc tacaacagaa atgtgattta ggtgacgata
      661 tttggtttga attttttctt gttcctttag gagccaattt tctaataact gtactgccac
      721 ttcattctcc tttttctaac cagttttcca agacttgata aactcctcct tggctgttgg
      781 ctggtgctag agcagtcgcc acagctttgg ctttctcatc agcattttcc atcgcatagg
      841 caattccagc catttcaagc atttcaacat cattttcact atcaccaaaa gccatgattt
      901 cttgggattt caagtcccag cgcttgagta attcctccaa gccccatgct ttatgaatcc
      961 cagcttggag gatatcaatg caaccatagc cactggatac agctcggaca cggccatcaa
     1021 agagagcatt gatttcttcc aaaaccgaac tcaaacgttc ctcaccaaca accatgctca
     1081 tcttgagcac accaccaaag aggtcagatg ttaattcatc cacaaattgc atccgttggt
     1141 agaatttttc aatcatttct ggagtcataa aactttcaag atctgtaaaa atcgtacctt
     1201 ccttgacaaa atcacccttc atccccgtta caacaaactg gtcctgacac gctcgaccct
     1261 tgaaatgagc caaagccttg ttgacaatgg cgtcatccca tgtctgagcc tgaatcaatt
     1321 cattgttttc aaaaatacga gcgccattag caacaaccag aaccactcga tccaccaagg
     1381 gactcagtag ttgtctcatg cggtgaattt cattgcccgt cgcaatgaca aaacgaatgc
     1441 ccctttgatc caactgatct aaaatctttt ccaatcgtgg gagatcaagc tgacctctag
     1501 catccagcaa ggtcccatcc atatctgtcg caattacctt aatcatcatt tactttcctc
     1561 tggattcaag ctaataccac ttcaatccca catgttcatt catttcttta taggctgtat
     1621 caattcgttc cttagtcgct tcatctaact ggtcacgatg actgccatcc tctataaaat
     1681 cttccaaaat ataagtttgg taatgataca agggatatcc ttctggtgaa aaggttccct
     1741 ttggtgttcg attgacccaa gtaggatgag ctttagctgt tccgatagtt gtttttccat
     1801 ccttcttctt gatggtgaca tccatgagaa caccacgttc agtccactta gcattctctt
     1861 catctcccat agattcaatc cgttgattgg aaatgaagtt ccccatttaa tagataatga
     1921 gtttcttatc tccatctttt tcaaccgttt cagatggttc aacaacgtga ggatgccctc
     1981 caaagataat atccgctccc caatcgatca tcttgtgata aagagctttt tgttcttcag
     2041 ttagttccaa tcgatactca acacccatct gaagcatgat aatggtgata tctgcttcct
     2101 tctctgcccg ttcaatttca accttcatct tatcttcgtt taaatctgaa agataacgat
     2161 tatagtcttc ctgagaaata tactgctcaa ttccattgaa accataggaa taggctaaca
     2221 atgcaacctt gataccattc acttccttaa tgaccagcag agcctgatca cgtggttcgt
     2281 gcgtataaac tccgattgga gtgattccag ctttctcaat aatatcggcc gttgaaataa
     2341 ctccctcaat ttgcgaatcc aaaatatgat tatgagctaa atccagcaca tgataacctg
     2401 cctccttaat agcatccata acttcagcag gagcattaaa gagaagataa cccgctaaat
     2461 aatgatcctt attaatggtt ccttcaaaat caccaatagc taaatctgct gcttagtccc
     2521 cttgcttgag ccaaggagtc acatactcaa aattttcatg aaagtcatag gtaccgtctt
     2581 cttttttagc tgaaaagaaa agtccatcgt ggtagagcaa atccccgtgg gccataattc
     2641 tggcagtttt ttcctcttcc tactcctgag acttttgctt agtcccctct tgagaaatag
     2701 tatctcgttt ttgactagtc aagggattcc ttggaagctt tcaaacaaca agaccaagcc
     2761 cattgataaa tcaactgcta gcaagagtat cgccacaaat cccttattgc tccacttgcg
     2821 ataactccta aaaagtttac caagcccttc ataaaacgaa aagctaaacc accctgattt
     2881 cgattttgtc ttctttgtat cttcattctc cctactttct tatgcaagcc ttttcttttt
     2941 attatatcac agataagtat ttctttcaca attgaattga acttcccatc tattttctat
     3001 aaatcctaaa tgccataatg ctttcaattc ttgtcatttt gtgatatcat gtagaggaaa
     3061 tgaactaatc cacagtggct tattccaagt ataccacttg ggctttggca gtagctaact
     3121 gcactaaaca taatataagg agagaaaatg tctgcaatag aacgtattac aaaagctgct
     3181 cacttaattg atatgaacga tattatccgt gaagggaatc ctactctacg cgcgattgct
     3241 gaggaagtca ctttccccct atctgaccag gaaatcatcc taggcgaaaa gatgatgcaa
     3301 ttccttaaac attcccaaga tcctgtcatg gctgaaaaaa tgggactccg cggtggtgtt
     3361 ggactggctg ctccccagtt agatatctca aaacgcatta tcgctgtttt ggtacctaat
     3421 attgttgaag aaggcgaaac tccacaggaa gcctacgatt tggaagccat tatgtacaat
     3481 ccaaaaatcg tctctcactc tgttcaagat gctgctcttg gcgaaggaga aggttgcctg
     3541 tctgttgacc gtaacgtgcc tggctatgtt gttcgccatg cccgcgttac tgttgactac
     3601 tttgacaaag atggagaaaa acaccgtatc aaactcaaag gctacaactc cattgttgtt
     3661 cagcatgaaa ttgaccacat taacggtatc atgttttacg atcgcatcaa tgaaaaagac
     3721 ccatttgcag ttaaagatgg tttactgatt cttgaataaa gaaaatcccg ttgcaagacg
     3781 gggttttgtg ttataataga ggcatgaaaa caaatgatat tgtctatggt gtccacgccg
     3841 ttaccgaagc tctccttgca aatacaggaa acaaactcta cctccaagaa gatctccgag
     3901 gtaagaatgt tgagaaagtc aaggaactag ctacagaaaa gaaggtgtcc atttcttgga
     3961 catcaaaaaa atctctctct gagatgactg aaggtgctgt tcatcaaggt tttgttctac
     4021 gagtgtctga atttgcctat agcgagctag attacatcct tgcaaaaaca cgccaagaag
     4081 aaaatccact tctattgatt ctagatggtc taaccgatcc ccataatctg ggttctatct
     4141 tgcgaacagc cgatgcgacc aatgtttcag gtgtcatcat tcccaagcac cgtgctgtcg
     4201 gagtaactcc tgtcgttgcc aaaacagcca caggtgctat tgaacacgtt ccaattgccc
     4261 gagtgacaaa cctcagtcaa accttagata aactcaagga tgaaggcttc tggacctttg
     4321 gaacggatat gaacggtact ccttgccaca agtggaatac aaaagggaaa atcgccctca
     4381 tcattggaaa tgaaggaaaa ggtatctcta gcaacatcaa aaaacaagtc gatgagatga
     4441 ttaccattcc gatgaatgga catgttcaaa gccttaatgc cagtgttgct gcggccattc
     4501 tcatgtacga agttttccga aatagactat aaaaaagttt ccagtcatct gattggaaac
     4561 ttttttatga ttaactatgt tctgtgataa acttgtaggc ttcttgacca gcgatagctc
     4621 catctccaac tgctgttgtt acttggcgaa ggtctttcaa gcgaacatct ccaactgcaa
     4681 agataccgtc gactgcagtc ttcatgtggc tatctgtcac aatccagcct gcctgatctt
     4741 ggatattcaa ttctttaaca aaatcgctaa gagggtccaa accaacatag ataaagacac
     4801 caccgaaggc ttgttctgtc acttgacctg ttttcacatt ttcaaatacg actgattcta
     4861 ctcggttttc acccttgatt tcccttacta cagaatccca gataaagctg attttttcat
     4921 tcgcaaaggc gcgatcttgt aaaacctttt gggcacgaag ttggtcacga cggtgaacaa
     4981 tggtaacagt cttagcaaaa cgagtcaaga agagggcttc ttcaacagct gaatctccac
     5041 caccaactac caataaatct tggtcacgga agaaagcacc atcacacaca gcacagtaag
     5101 aaacaccacg actgttcagt tcttcttctc caggcactcc caaaggacgg tgtttagaac
     5161 cagttgctac gataactgta cgtgtttcat atgtttggtc atcagtcatc actttcttaa
     5221 aatcaccatg gtcttcgaca ttttcaacat aaccataaat gtgctcaaca ccaagatttt
     5281 caagtggttc aaacatcttt tcagccaatt caggtccact aatattagcg tatcctgggt
     5341 aattttcgat atcagatgta ttattcatct gaccacctgg cagaccacct tcaatcaaag
     5401 ctacttttag attgcttcga gcagcataca aggccgcagt catccctgca ggtccagcac
     5461 cgataataat agtatcgtac atatagattc cttctttctt gttgtaacta tctttattct
     5521 aactctttct tgtcaatcac gcaaggatta tgccttgaaa acaagaatta aactcataac
     5581 cgcaaaggat aatactggga caaccaagac tccaatcata atcatatcat agagatgggc
     5641 ttggcgagcc ttggctgtct tatcaactcc cgacatggct ctcagaccaa tccaaatccc
     5701 taaaaaaatc agaacaagga tggtggtcaa tatcaaactc tcgaaatata aagaaaatag
     5761 ttgcagtagc atgatttctc tcatttctat cttttttaaa gagtaaactc agctagtcca
     5821 actaactgag ttttccttta tctattatat caaatataag tccgtttgta actagcgaag
     5881 aattcttttg tccgctcttc tttaggggtg tggataatct catccggagt tccagactcg
     5941 atgattttcc ccttatctaa gaagagaatc ttatccgcaa cttgggctac aaaggacatg
     6001 tcatgactga ccaaaatcat ggtctgacct gacttagcag catctgcaat agacttttct
     6061 acttcaccga ccaattctgg gtcaagggct gaagttggtt cgtctaagag caaaacatct
     6121 ggtttcatag caagcgcacg cgctagggca acccgttgct tctgtccacc tgataaatgg
     6181 cgaggataat ggttttcacg gtccgaaagc ccaaccttag ccaactcttc cttggcaatc
     6241 ttagtcgctt cttggtcaga taatttcttg acaacaacta agccttcttt cacattatca
     6301 agtgctgttc ggcgttcaaa caaattaaac tgttggaaaa ccatagacaa cttacggcgt
     6361 agggcaagga tttcttcttg agtgatttta gaaaaatcaa ctgaaaaacc atcaatctga
     6421 atagagccac tgtcaggtgt ttcaagataa ttgagactgc gaagaaaggt tgattttcca
     6481 gctcctgaag aaccaatcaa ggctacaact tccccttttt gaatatccaa gtccagatga
     6541 tccaagacag tctgtcctga aaaggatttg cttaaattcg aaatcttaat cattaacgaa
     6601 ggtctccttt cacatctgtt tgcactgtat caggtgcaga aatagccatt tttctctcga
     6661 tgaaacgacc gaggctttca attccgatat tgactaccca ataaacaagg gcaacggaga
     6721 tgaagcgttc aaaatagcga taatcagctc cacctagaat ctgagcttgg gcaaagactt
     6781 ccacaacacc cgcactaaaa gctagagatg ttcccttggt caaaccgatg agggaattaa
     6841 tcaaggttgg agtagctacc accgctgcat taggaataat cactcgtcga taaacttgcg
     6901 ctcgggtcat acccagactg cgtgccgcct caatctcacc aggattaact gagagaatgg
     6961 ctgcacgaat ggtttcacta gcataagctg cctcattaaa ggcaaaagcg acaatcgcaa
     7021 aagctgcagc tggaatcgca ttgatattga gaccagttcc ccattgctga ttgagggctt
     7081 tcaaagccaa agggattccg tagtaggtca acatgagttg caccaaaatc ggtgtccctt
     7141 ttaagaaact aacaaagaag gcctgcaagg gatataaaat cttgacacga ttgatcttca
     7201 caatggcaaa aagaagcgcc aaaaccaagc caaaaagggc accaccaatt gtcaacataa
     7261 ttgttgttgg aagttgttgg acaattctag ggattccatc aaagaccgaa cgtaggctaa
     7321 acagcttgcc atccggaatc aattgcatca agttttggta ccaatctgat gctaaaatcg
     7381 ttgtaacatt cataaaaaca tcctcttctg ataatgattc attctaccat gcttctgccg
     7441 tcaatgaaat tatttgctac ggtcagatac agactttgtc tacctactag tttatcatac
     7501 tttgaaacag ggaagttata tattttatct atcaaagaga tagataaaaa ctatttcagt
     7561 cccagttctt cataagattt tcgataaccg atttgcttaa cagttccatt ttctactaaa
     7621 atgggccgtt ttaacaacat accgtcactt gctagcaact cagccgcttc ttggtttgac
     7681 aaacttccta ccttatcttt tagccctaat tcacggtatt tgataccact ggtgttgaaa
     7741 aattgcttca attcaaatcc tgaggtttct agccaattca aaatgacttc ttggctaggt
     7801 gtttcttcca cgatatggac ggctttatag tccacaccta attgatttaa ttcttgtttt
     7861 gcttttttac aagttgaaca tttggggtat tcgataaatt ctaacatgtg cttctccttc
     7921 tatttgttta tatctttaaa atctacgcct tcatgctcca agagccaagc tttcttttcc
     7981 actcctgcag catagcctgt cagacgcttg cctgctccca acacacgatg acaaggtact
     8041 aggatagacc aaggattgcg tcccactgct ccaccaattg cttgagcaga agccacttgc
     8101 aggtcttgag caatttgtcc ataggtcact gtctgaccat aaggaatgcc ctgtaaatag
     8161 tcccaaactc tcttttcaaa attcgttccg attggcgcca agagcaagtt ggataaatcc
     8221 tgaggcttgc ctttaaagta atcatctaag caagcaataa ctgggtctaa aataggatga
     8281 ctaacaactt cttctatcgt ttcatctcct agtcccctct caaaatgctt ctgctcctga
     8341 acccaaattc catacaaata atggtcatca gctacaagtg atagaatacc aattggtgag
     8401 gagtagagga tttttacata cttcttttgc attatttctt taatttttga taggccaagc
     8461 gttcgccatc tactggcacc ttaccaactt gtttaaaacc aagtttttca aaaatatgtt
     8521 gcataacctt gttttcagca tgcgtatctg agcgaaaatc aagataatca aaaccttcaa
     8581 tcaagccctc taagaaggtt tgagcaactc cttttccctg cacatctgct gccacagcaa
     8641 tacggtgaaa gactagatac tctgactctc cagcttgcca gtttccctca taaatagctt
     8701 cataggcctc ctctggactc ttggtcacag cagcataggc tagcagttct ccctcttcca
     8761 aggctacgta ggcttgccct gagatgatat catcgataat aacatctgca tttggatagc
     8821 cattttgcca ctggtcacta ccagcatctg ctaaacattt tttggcatcc tctatcacct
     8881 gcatgatggc acctacttca tttggaaaag ctaaacgaat ttccatcttt tttcctcatt
     8941 tctgactagt ttctcccatc atagcataat ttaagtcttt ctacaagcag ttaaaacttg
     9001 actttgtatt tttgttattt tataatctag ttatcaaaac tatataaaaa ggagttgaaa
     9061 atgaaaacta ccttttccta cccaaaatgg gcagaaattc caaacattaa cctctatctg
     9121 gaccaggttt tgctctatgt caatcaggtc tgcgccccta tctctcctaa taaagacaaa
     9181 ggcctaacag catctatggt caacaattat gtgaaaaatg gttacctgac aaagcctgac
     9241 aaaaaaaaat accaacgcca acagattgcc cgtttgattg ctatcacaac cctcaagtct
     9301 gtattttcta ttcaagaaat agcccagaca cttaatactc tacaaactca agcaagttca
     9361 gaccaactct acgatgcttt tgtggactac atgaaccaag gaattgaccc agctaaccct
     9421 attatccaaa ccagctgcca aaccgttaaa ctctatcatc aaactctaga cttaatcgat
     9481 catactcaag aggaggtaat ccaatgaaca ctagtcttaa actcagcaaa caactcagtt
     9541 ttggagagga gattgctaat agcgtgaccc atgctgtggg tgcagtcatc atgcttatct
     9601 tgctgcctat ttcatccatc tatagttatg aagcacacgg atttttatcc tctatcggcg
     9661 tttccatttt cgtcatcagt ctctttctca tgttcctatc atccaccatt tatcactcta
     9721 tggcctatgg ttcgacccac aaatatgttt tgcgaatcat tgaccattct atgatttacg
     9781 ttgccattgc cggctcatac acgcccgttg tcttgacctt gatgaataac tggtttggct
     9841 atctgattat tgtcatccaa tggggaacga ccatctttgg tattctctat aaaatctttg
     9901 ctaaaaaggt caatgagaaa tttagccttg ctctttacct gattatgggc tggttggttc
     9961 tggctatcat tcctgccatt atcagtcaaa cgacacccgt tttctggagt ctcatggtaa
    10021 ctggcggact ctgttataca gttggagctg gattttatgc caagaaaaaa ccttatttcc
    10081 acatgatttg gcatctcttt atcctagctg cgtccgcact ccaatacatc gctattgttt
    10141 attacatgta aaaaagttga gaagttcaat ctcaactttt ttctttacac atattgataa
    10201 agtactggtg caagcgcaca tcatcagtca attctggatg aaaagaactt accaacatat
    10261 ttttttcttg ggctgcaaca atttgattgt tcactgttgc tagaatttct acaccctcac
    10321 caacactact gataatcgga ccacggataa aggtcattgg aatcttgcca actcccttac
    10381 attctgcttc cgtgtagaaa cttcctaatt ggcgcccata agcattacgc tcgaccacca
    10441 tatccatagt tcctagatga ctctctttct gagaagtgat ttccttagcc agcaaaatta
    10501 agcccgcaca ggtcccaaac actggtaagc cagatagaat ggcttctcgg atgggaagta
    10561 gcatgttctg gtcacgtaag agcttgccca tggttgtaga ctcaccacca ggcaaaatca
    10621 aacccgacaa gtcactctga tcttgctgaa aatcatctag atttctgagt tctacactct
    10681 cgacacctaa ttgatctagc acttttgcat gttctgcaaa ggccccttgc aaggccaata
    10741 ttccgatttt catctatttt cctcgttcag ccatgagaat ttggatttca ttttcattaa
    10801 taccaaccat ggcttctcct aaatcttcag agatttgagc taggatttga ggattacgga
    10861 agttagtcac agccttaaca atggcactcg ctcgtttaac aggatctcct gacttgaaaa
    10921 tacctgaacc gacaaagacc ccctctgccc ctaattgcat cattaacgca gcatctgctg
    10981 gcgttgcaac acctccagca gcgaaattta caactggcaa ttttccatgt tcatgaacat
    11041 attggaccaa ttctacaggg acttgcaaat ccttggcagc aacataaagc tcgtcctcac
    11101 gtaagttttg aatgcggcga atttcctgat tcatcatacg catatgacga acagcttgga
    11161 cgatatcccc tgtccctggt tctcctttgg tacgaatcat ggaagcacct tcagcgatac
    11221 gacgcaaggc ttcacccaaa tccttagcac cacagacaaa aggaacttgg aattctttct
    11281 tgtccacatg gaaacggtca tcagctggag atagaacttc actctcgtcg atataatcaa
    11341 tttcaatagc ctctaaaatc tgagcttcaa caaaatgccc gattctgacc ttagccatta
    11401 ctggaatact aaccgcttct tggatttcct taatcatctt tgggtcgctc atgcgggaaa
    11461 ctcctccagc tgcacgaata tcagccggaa ttcgttccaa ggccatcaca gctgccgcac
    11521 cagcagcttc tgcgatacga gcctgttcag gattctgcac atccataata acaccaccct
    11581 tgagcatctg tgccaagttt ttatttagtt cataacgatt ttcagtcatt tgttttatcc
    11641 tcatcatttg tattggtagc taccttttca ttttaagtca gaacagaatg aagcacaaga
    11701 taaaaaaact ataattgtat ggggacagat tggctaaaaa ctatctgacc ttaacttaag
    11761 gccagatagc tcattcattt ctatttttca gccgtaaggg cagccattgt gatgtagttg
    11821 tatggtttgt tgaagtgtgg caagaagaag aggtctgtca atgccaattt atcaattgtc
    11881 acatgctctt ggatagcaag tgagaacatg tggattccca tgctaattgc aatatcatgt
    11941 gaaaccattt gggcaccaag aatttcacgg ctatctttgt caaagacaat cttaattgct
    12001 acttcatggt tgtcatgttt catgaattct ggtttttgaa gatcgttaaa gcctgtttca
    12061 gttgcgttgt aaccagcagc tttcgctttt tcaagagtca aaccagttga aaccatgtga
    12121 agaccgtaga ttgagatacc atttgaacct tgaacaccga ttccttccaa ttcatgtcca
    12181 caagcattgt aggcaccaac gataccagtg cgaacagcat ttgaagcaag agcgatatag
    12241 cttgtatctt tacgagcatt gtcataaaca gtcgcacagt caccaacagc gtaaacgcct
    12301 gggatagatg tttcttgttt cttgtctaca aggaaggcac cgttgcggaa gagttcgatc
    12361 ttaccatctg caagggctgt gtttggacgg aaaccaactg caaggataac catatccacg
    12421 tcaaagcttt ctttgtcagt aatcaagcgt tcaactttac cgtcaccttc gattgcttta
    12481 acagtttgac ctagagccaa gcggatgttg tgatcttcca agttcttcgc catcatttgt
    12541 gtgaagtctt tgtcatagta accgttcaag acagtatcaa cgatatcaac aaggacaact
    12601 tcttttccaa gacgttcaaa ggcttcagca agttcaacac cgatgtaacc accaccaaca
    12661 acggcgatac ggtcgaggtg ttggctcttg tcagaaagtt tattgataac ttcttcagca
    12721 ttttggtaca atttcacgaa ttgtacgttt tcaagagttg ctttaaattc gcggtttcct
    12781 ttaacaattt caacaccttc gattggtggc aagattggtg tagaacctgt agcgaaaatc
    12841 aatttttcgt atgattcttt gtgctctttt ccttcaactt ccgctgtaac tactttgtta
    12901 tcatagtcga ttgaaagaac aggtgagttc atgtaaactt tagcaccttt agcttccaat
    12961 ttttctttat cagaatagaa caagccttca gcaccgtcaa tttgttcacc aatccaaaga
    13021 gccattccac atcctaggaa agagatgtta gagttttggt caaatacaac aatttcgttc
    13081 tcatttccaa aattatccaa catggtattg atacatgctg taccagcgtg gttagcaccg
    13141 actacaacga ttttactcat aaaaatttcc tgccttcatt ctaaatttac cttactagta
    13201 taccatttac cgttagcgtt tacaaggggg gtactattat ttcagcattt tttatcaaaa
    13261 ataattcccc atttatcctg ttttcaagcc tcatgaattt tatgagcata tttcttgact
    13321 ttttccaaat tttgtttgtg aaaattctaa ctttatggta ttataataac atgaaaatga
    13381 aatgtaaggg cttcaattta gatgattatt tgatattaca acttattttc tataagagaa
    13441 aggagttggt agcttgactc ttcgttcaca ttctgaacgg ctaatgggga ctactatcac
    13501 tatttcatta gtggatgagc aagccgatat ctttctccaa aaatcctttg acttgctcaa
    13561 agaacttgaa taccgcttca acgccaatag tcaagaatct gagttgatgg aaatcaatta
    13621 tcaagcagga atagcccccg tcacggttca tccagacctg tttgagctga tttcacttgg
    13681 gttagagcat agcctagcgc tctctagcca cctcaatatc agcattggtc ccttaattca
    13741 aacctggcgt atcggttttt cagatgccaa ggtcgcccag cctcaagaaa ttgaatcggt
    13801 gctgcctcta atcaatcctc atggtatcga gttagattct tctacttcta ctgtgttttt
    13861 aaaacagaaa ggaatgaaga ttgatctagg ttgtttagcc aaaggataca gtgcggataa
    13921 ggttgcccaa tttcttagga aagagggggt gacttctgcc ttgatcaatc tgggagggaa
    13981 tatcctgacc attggaaaaa atcaggcaag aggggataac ccatggcaaa tcgggattca
    14041 agacccagcc aatcctaggg gaaatcattt aatgaccatc cctgttgtca ataaatctgt
    14101 cgtgacttca ggcatttatg aacgtcacct gaccgtcgat ggacaagatt accatcacat
    14161 ttttgacagt caaacaggat atcctgttga aacggaacta gcgagtctaa caatcatctc
    14221 tgataaatca gtcgatggcg aaatctggac gactcgactc tttggagaaa gacctgcttc
    14281 tatcctctgg caagtcgaaa gtttggaggg catcgaagtc atcctcatcg ataaagaagg
    14341 ccacctaagc tgttcttcag gaattcctac tctatagaga caactgtttc aatggaattt
    14401 taaaattgta ttatgtaaaa agagaaagga aatctcatgt taaaacttat tgctattgtt
    14461 ggaacaaatt caaaacgttc tacaaaccgt caattgcttc aatacatgca aaaacacttt
    14521 gctgacaaag ctgaaattga gcttgttgaa atcaaggcca ttcctgtctt caacaaacca
    14581 gctgacaagc aagtacctgc tgaaatattg gaaattgctg ctaaaatcga agaggcagat
    14641 ggcgttatta tcggtactcc tgagtatgat cactctattc cagctgtttt gatgagcgct
    14701 cttgcttggt tgtcttatgg tatttaccca cttttgaaca aaccaatcat gattacaggt
    14761 gcttcttacg gtacgcttgg ttcatctcgt gcccaattgc aacttcgtca aatcttgaat
    14821 gctcctgaaa tcaaggcaaa tgttcttcca gatgaattct tgctctcaca ctctcttcaa
    14881 gcatttaacc caagtggcga cttggttgac cttgatgtta tcaagaaatt ggatgccatc
    14941 tttgatgact tccgtatctt tgtaaaaatc acagaaaaat tacgtaatgc acaagaatta
    15001 cttcgcaaag atgctgaaga ctttgactgg gaaaatttgt aagataggag accaaaaaga
    15061 atgaaatttg ttggacttgt tggatcaaac tacgatcaat catataaccg taagctcttg
    15121 gaatttatcc gtcgccactt caaactcaaa tttgaattag aagttcttga aattgacgaa
    15181 gttccaatgt ttaaccaaga tgaaaaatgg gatgaaagtt tccaattacg ttatttatat
    15241 aacaaaatta ctcgtgcaga tggtgttatc attgctactc ctgagcataa ccacactatc
    15301 tcagcttcac tcaaatctgt acttgaatgg ctttcatacg aagttcatcc atttgaaaac
    15361 aagcctgtta tgattgtggg tgcatcatac tatgaccaag gaacatcacg tgctcaagtt
    15421 caccttcgta aaatccttga tgctccaggt gttaatgcct acacgcttcc aggtaacgaa
    15481 ttccttcttg gtaaagctaa ggaagctttt gataacaacg gaaatatcac caacgaaggc
    15541 actgttaaat tccttgaaac ttgcttagat aactttgtta aatacgtagg agtcgtttcg
    15601 aaattgaaaa aaccaaaacc aatcgaatca gaagacttgg attgtggaaa accaatcgct
    15661 acaaccatca cagaagttga ccctgacgat ccagaatggg tagaaaaagt tgcagcaatc
    15721 actggtgctg tttctggtga tacctatgtc aaattggacc acggtatcct aacagttaac
    15781 caaattgata tgttcttgaa agctatgcca tttgaattga catacgctga cgacaacaac
    15841 caattcctct actacaacaa cgctcaccaa gatccagaca ctatgttcgc taaacgtgta
    15901 ccacctcaat caggtagccg tatgtcgact gttcatggtt ctcttccacc agcacgtatg
    15961 aagaacgtag agtgggttat cggaacactt cgcaacggaa accaagaata cgtccgtacg
    16021 atcgttccag gttctcctgc aggtgttatc aacactcaca actaccaagc aatgtactat
    16081 cctgatggat catacgctgg tatcaatgaa atcgtcttta acttccaacc atggcttgac
    16141 tggtacctaa aagagactgg tcaacgtttg gtgggtggta gcggaccatt tgctcctgct
    16201 gccggaggtc atggagacgc cgatgctact tctggcgctt ctgattcagg ggatgctgga
    16261 ggccacggtg gtgacgcaga cgctacagcc ggtgcaagtt actaatgata gtttttagga
    16321 accattatgg ttcctttttt agcgaaaaaa gactagcaaa cacaaactgc tagtctcttt
    16381 gttatcgttt catctatctt tgttaaaaga aagagtacat attatgaatt tgggtcatca
    16441 agactacggc catgtaatcc tttttcacgt tgtacttggc gtagtttttc tggtgtaaca
    16501 tcgtttcctt cttcgtccac aattttgatt ccttcaatgt gatgacgaac agcgcgacga
    16561 taaccttcga tgtactcctc acgtagtttg gcttgttcca ctttttcttc tagtgttaag
    16621 ccttctgttt ttttcttttt agcaagctca ttgatacgag caattttttt cggatccatt
    16681 tcttctcctt tgtaataaga taaagtccgt ttatcaaatt ttatttagtg ttaatttggt
    16741 taaaacaagc aaacttagct gctttcttgg ttagttgtga caagattgcc aaacgatttt
    16801 gacggacagc ctgatcttca gccattacca tagtattttc aaagaaagca tcaatgactg
    16861 ggctaagcgc aaagagttgt ttcaattgct gacttgcagg tcctgataaa acgagtgttt
    16921 ctactgcttc tgccaaagct ttttcttggt cattctcaaa tagtgctgaa tcaactgtag
    16981 cacccccttc tgccttctca gccaggttaa aggcacgaga aagtgattca acagatggtt
    17041 taaaatcttc ttccttgctt acttctacga gagcacttgc tgcttccaac atatctgcca
    17101 caacaaagtt tgaacctgca agaactgctt ccttgatatc ttttggagta gagcccatca
    17161 tcttatcaac acgagccttg ataaagtcca taacctctgc tttattttca taagtcaaac
    17221 tgtcaaattt caatgcataa aggctatcaa tcagctcatc catagcaatg tgccaaccaa
    17281 aggcatccaa gatacgaacc acaccttgag ttgcacgacg aagggcataa gggtcattag
    17341 aacctgatgg aatcaatcct actgagaaga aactcaaaat cgtatccaat ttgtctgcaa
    17401 tggctagaac tgcgccgacc ttgctctctg gaagttctcc ttcagctgat gtaggcatgt
    17461 agtgttcacg aatagcagct gccaccgctg gagtttcacc agcaagaagg gtgtattttt
    17521 cacccataat tccttggagt tcgtcaaatt caccaaccat acctgtcaat aagtcaaact
    17581 tgtaaatggc tgctgcacga gcaaggtcaa ctgtttcatc cactgacaaa ccagcttttt
    17641 ctgccaaaag tacagtgatt tgacccgtac gaatcatgtg ttcacgaagg gaaccaatct
    17701 tctcatggaa ggtgacattg tttaattttt caacaagatc tgaaatcacc aatttttggt
    17761 cttcacgcca gaagaattct ccgtcttcca agcgggctac caagactttt tcatttcctt
    17821 tgatgacatt tttcaaacgc tctgcgtttc cgttacgaac agaaatgaag tttggcaaga
    17881 gttttccatc ttgatcacga acaacaaagt aacgctggtg ttccttcata gaagtcacca
    17941 agacttcttc tggaacttca aggtatttag catcaaaact tcccatgaag gcagttgggt
    18001 attcaaccaa attcaagact tcattcagca aatccgcatc aatttcgata cgtacaccat
    18061 gttttgcctc aatttccttg atttggtcaa caatcatttg ctcacgttca catggatctg
    18121 cgattacaaa ctgcttacga aggtcttctt catagctcaa tgctgactga attttggttt
    18181 ctttccccaa gaaacgatgg ccacggctca cacgacttcc cttgatatca aggaaatcca
    18241 agtcaaactc ttgctcatcc aagagaacag ttaaagtgtg gacaggacga atgtattcaa
    18301 agctgtttcc agcccagtgc atgctgacag ggaaagtcaa tgacttcaag acatctacaa
    18361 caccaggaac aatggcttca actgcttgac caacttcttc cttagtgaca tagacatatt
    18421 cttcaccctt gatttcacgg aattcgatat cttcaacagt caagcctttt ccacggacaa
    18481 atccttgagc tgctttggtg aagtttccat cactatccaa ggcaattttc tttgctggtc
    18541 ccttgaaatc ttctgtcaaa tcagactgtt tgtctgataa accagtcaca cgaacagcca
    18601 aacgacgtgg tgttgagaag gtttgaatgg cttcaaaaga caggcgtttt cccttgagga
    18661 aggctgccat tttttcgcct agttgttttt cacttggtgt gacaacatag gctggtaatt
    18721 cttcaagacc gagttctact aataagtttt ttgtcatgtt ttttctccga aaagtatctt
    18781 tttattttcc agtttgcctt atgtgattgg cacgcaagta accaacttcg ttgtttcatt
    18841 gctaaaattg gagccaaaag tctccaattt tgcctagtat ccttagtttc actaaggata
    18901 ccttcatcac tgcggcaact ggctcagggc tcactctgtt cgcccatttt cgtaatttgt
    18961 cactctcttt attctgcgtc ttctgctaag agtttagctc gtgtttcttc atccaaaagt
    19021 gggtagccta gtcgtttgcg ttctgcgaca aaggttttgg ctacgacacg ggccaagtta
    19081 cggatacgag cgatatagcc tgcacgctct gttacagata cggcaccacg cgcgtcaagc
    19141 agattaaagg tatgtgaaca tttgagaaca tagtcatagg cagggtgtac caagccttct
    19201 tctaatgcac gaccagcttc tttttcaaac ttatcaaaat tttcaagcaa catttcttgg
    19261 tccgaaattt caaatgaata ttttgagtgc tcatactcag gctggataaa gatttctccg
    19321 tattttacac catcagccca ctcgatatca tagacagagt ctacttcttg aatgtaagaa
    19381 gccaagcgct ccaaaccata ggtaacttcc gcagtcacag ggctagttgc caatccaccg
    19441 acttgttgga aataagtgaa ctgagtgatt tccattccgt caagccaaac ttcccaacca
    19501 agaccagctg aaccagttga tgggttttcc cagttgtcct caacaaaacg aatatcgtgc
    19561 tccaaaggat tgattcccaa tttttccaaa gactcaaggt aaagttcttg gatatttgat
    19621 ggagaaggct tcatgaccac ctggaattgg tggtgttggt agagacggtt agggttttcc
    19681 ccataacgac cgtcagcagg acgacgtgat ggctctacat aagctgcatt ccatggctca
    19741 ggtccgatag cacgaaggaa agtgtaagga ctcattgtcc ccgcaccttt ttcattgtca
    19801 taagcctgca taagcataca accttggtca ttccaaaatt gttgcaaagt caaaataatt
    19861 tcttgaaatg ttaatttctt agacattgaa tactccttaa taaaaaatga tttcttgcga
    19921 cacgagtctg cctcgtcgat tatacgaggc aagacgagtt agtactgcgt ctagctcagc
    19981 accctagtgc tgagcgtggg gcagtccgac tgtaaggagg tttctgccac tgacgcagtg
    20041 aaatgtcaat ttcttagaca ttgtttactc ctttttatat aaattacttg cgacacgagt
    20101 ctgcctcgtc gatcatacga ggcaagacga gttagtactg cgtctagctc aggcactcta
    20161 gtgctgagcg tagggcagtc cgactgtaag gaggtttctg ccactgacgc agtgaaatgt
    20221 taatttctta gacattgaat actccttaat aaaaaataat ttcttgcgac acgagtct
//
LOCUS       DAVKGC010000029        19610 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKGC010000029 DAVKGC010000000
VERSION     DAVKGC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19610
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.029"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(144..758)
                     /locus_tag="VW049_000847"
     CDS             complement(144..758)
                     /locus_tag="VW049_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9432003.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1574..1843)
                     /gene="rpsO"
                     /locus_tag="VW049_000848"
     CDS             complement(1574..1843)
                     /gene="rpsO"
                     /locus_tag="VW049_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9432004.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(1984..2613)
                     /locus_tag="VW049_000849"
     CDS             complement(1984..2613)
                     /locus_tag="VW049_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9432005.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKDQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            3483..3623
                     /locus_tag="VW049_000850"
     CDS             3483..3623
                     /locus_tag="VW049_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9432006.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            3630..3806
                     /locus_tag="VW049_000851"
     CDS             3630..3806
                     /locus_tag="VW049_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9432007.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            3864..5807
                     /gene="thrS"
                     /locus_tag="VW049_000852"
     CDS             3864..5807
                     /gene="thrS"
                     /locus_tag="VW049_000852"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9432008.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSIGNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5856..6830)
                     /locus_tag="VW049_000853"
     CDS             complement(5856..6830)
                     /locus_tag="VW049_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9432009.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6823..7500)
                     /locus_tag="VW049_000854"
     CDS             complement(6823..7500)
                     /locus_tag="VW049_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9432010.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7656..8729
                     /locus_tag="VW049_000855"
     CDS             7656..8729
                     /locus_tag="VW049_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9432011.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8820..9122)
                     /locus_tag="VW049_000856"
     CDS             complement(8820..9122)
                     /locus_tag="VW049_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432012.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9190..9627)
                     /locus_tag="VW049_000857"
     CDS             complement(9190..9627)
                     /locus_tag="VW049_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9432013.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9703..10545)
                     /locus_tag="VW049_000858"
     CDS             complement(9703..10545)
                     /locus_tag="VW049_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9432014.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10657..11307
                     /locus_tag="VW049_000859"
     CDS             10657..11307
                     /locus_tag="VW049_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9432015.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11427..12134
                     /locus_tag="VW049_000860"
     CDS             11427..12134
                     /locus_tag="VW049_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU9432016.1"
                     /translation="MKKSLKIFATSKWFDLFGVALVVGIAIASGYLNSRLDKFVDWGP
                     WTALVPFGLISVTNVGISMLSTRFTGKLSKWGNYFSIVNTILSGAIDYILGNKAAIIT
                     YPVTFLIYTFAIKKWKASQEGRPNQMSQKQVKLTVIIISIIAFLFAFVTNYIGYEGKM
                     NLLAYVTTIAFALSLIANALNALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFY
                     ILNAIGALFVWNDEEVR"
     gene            12265..12678
                     /locus_tag="VW049_000861"
     CDS             12265..12678
                     /locus_tag="VW049_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432017.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFIIRLETSFEWLLLSGLIFFLA
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            12697..12883
                     /locus_tag="VW049_000862"
                     /pseudo
     CDS             12697..12883
                     /locus_tag="VW049_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13150..13593)
                     /locus_tag="VW049_000863"
     CDS             complement(13150..13593)
                     /locus_tag="VW049_000863"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9432018.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(13623..15845)
                     /locus_tag="VW049_000864"
     CDS             complement(13623..15845)
                     /locus_tag="VW049_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9432019.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            16839..17468
                     /locus_tag="VW049_000865"
     CDS             16839..17468
                     /locus_tag="VW049_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9432020.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(17591..19483)
                     /gene="pepO"
                     /locus_tag="VW049_000866"
     CDS             complement(17591..19483)
                     /gene="pepO"
                     /locus_tag="VW049_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9432021.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPILSLLKEFQELETFADF
                     IAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         5744 a         4447 c         3631 g         5788 t
ORIGIN      
        1 ttataagaat ctcctaatca aatcaaaagc cactctacaa tagagtaact ttaagattgt
       61 ccatttatac tcaatgaaaa agccctctga aaatcagaga gctgatttga gctcgggcta
      121 aaatcctagt gaaacaaaaa aatctacacg atcagaaaag tctctatcgt gccattttca
      181 tacatgatgt atagtccaag tagaatgaat actaagggca caatgattcg ctcgtatttt
      241 tcaattgtct cgaatattaa cggaatagag gataacaccc tactaatctc gcaaaagata
      301 attatgccga ttacaaacac aagcaaggcc acgagggtct gtgaccaatc taacgaagca
      361 aaataaggta tatagatacc taaattatct ccgccagacg caattgtcag caatgtaact
      421 gtccaaaaca gttgatttgc cttgctttgt tctaatcttt caataatttc ttcctcttct
      481 tcttccgcat cttctccaac aattgcaaag cgaatcccta aatagatagg gattaaacca
      541 agcaatccaa ccatccattc ttcaggcacg aaattaacga cataagcagc aactaaactc
      601 gcccctacaa gtaagcctgt gcctagatat tgccccgcat aaatatgcca tttctgttta
      661 ttctgtgata gctgtgcaaa taaaataatt aaaataatta aataatcgat actggtggaa
      721 atataaacac caatagcaga tatgattgtc tgtcccataa aaaaaacctc ctgtattagt
      781 cagtaataaa tcagcagatt ttaggagagc acagacacat taatttagct atcgctagtg
      841 ggtaatgtcg aacgtaggaa agcattttac tcctcctcaa aacgtagtta caaagtaatt
      901 tctaactgga attcagtgac tacttccatg taaagtataa catactatac tttactttgt
      961 aaagagtggg ctcttctaag gggcttgcat acagctactc ggcttgaaaa gccgagtaac
     1021 tgcgcacctt tatggtgtaa ttagctgata ccgtttgagg tttttgtagt ctccaaaatt
     1081 gttaccgata aaacctcaat aaaaaagccc tctgaagtca gagagctgat ttgagcccgg
     1141 gctaaaatcc tagtgaaaaa gatgaaactc cttgtgctca tcgaacactg tgtcatttcc
     1201 ctattttcat acggattttt gacgccctta gcatcatgat tcttgctaga taaatcgttt
     1261 atagactagg cgacgaggcg aagatcgtac gaaactttca gtgaaacaaa aaaaatctcc
     1321 ccgaagggag aaaagctggt ttattttacc ttacagtgct aggaaccgta accaaagatt
     1381 atacttgagt atatcgaggt aaggtgacaa cgcaaaaaag ctacccaatt tagagtagct
     1441 tcattatcaa gatatgctca attgaacacg gcctaagcac ttggcaaaaa agataaaatc
     1501 tcctagaagc tgaagtttct tcgtcaattt tcctattttt gctttgtgct tttgacagca
     1561 tttgtatctt gaattagcga cgaagtccta gagagttgat caactcacgg taacggttaa
     1621 cgtcgttttt acgcaagtat gcaagcaagt tacgacggcg accgattttt ttcatcaatc
     1681 cacggtaagt agcgtggtct tttttgtgtt gtttgatgtg ttcgttaagg tggttgattt
     1741 cccaagtaag gacagcaact tgaacctcta ctgaacctgt atcaccttcg tgacgtgcat
     1801 attgtgcaat gatttcattt tttttctctt ttgagattgc catgatgttt ctccttttta
     1861 tttggcttca tccgagtgac aggttggcat gcctgtaacc aagaagaagt tatttgtctt
     1921 tacgacaatc cctattttac caagatttaa attttctgtc aaacattaat actctcatag
     1981 ttattatttt tcaagtaaag agatacgttc ttttatatga tgaccttttt caagttcatc
     2041 taccaatcca atagcataat ctgcataact aatctggctt attccttttt cattagtagt
     2101 aaaaatttct cctgctagaa tgtagtttcc tgttttttca ccatcgggaa taaaatctac
     2161 agcaggactg acaaaagtcc agttgagatt attttttgta cgaagaagat ccaattcatc
     2221 cgcttgatct tttgctagtg acttaaattc ttcaggaaaa tcagacgtat ctaaaagtcg
     2281 tgtcgttttt gtttcatcta tataaagact tcctgctccc cctacaacta gaaaacgtgt
     2341 ctgtgttcca gcaagaagct gattaaatag ttcgattgat ttgctgtgga gcggtagcgt
     2401 atctggtgta taagcaccaa acgctgaaat aacagcatca aatccagtaa gatcatcttt
     2461 tgtcaactca aataaatctt ttttaataat agactcagct tgacttttgt tttcagaacg
     2521 aacaatagcc gttacttcat gtcctcgttt gactgcttct tcaacaattg ctttccccgc
     2581 ttgtccattt gctgcaataa ctgctagttt cattttttat acctctcttg ttgtaattgt
     2641 tttagttaca ataattgtga cactcttaat aatcaatgtc aatagtcttg cttaattatt
     2701 atcaaaatat ttctaccaag aaaactaact atgattctag tgaaaagaaa aatcttcttt
     2761 gtcaacaaat ttactttctt gttttaaaca tgctataata atcatagcaa gagatctaag
     2821 ttgtctgttt ttttaaaacg aggtgattat catgcgtaga ttctattccc atctccccta
     2881 ctatctggtc atattattct tttattggcc actttatgag ttgttcttac tagttgtttc
     2941 tgaccccctt acactcaagg aactctatat aaacaatctt ctcttcttta cacctctggt
     3001 aatcttgatt atatcgttac tctatagcta ccgtttccgt ttctcacttt gatggttagt
     3061 tggtaacgga ctgctctttt actttactat cataaccttt ggtgagttta tactaattta
     3121 cttgctaatc tatgaaacag ttgctctggt cggcatggat tctggtatta gcatcaagca
     3181 tattctacaa aaaatgaaaa acaaaaaact ttcacaaaat ccttgaaaaa tctcacaatc
     3241 atgctataat aatccataga gacaagtcac ttagtccctt tctactagag agtgcgtggt
     3301 tgctggaaac gcataggaag tctaaactga tactactctt gagtttttta tgaaaacata
     3361 aaacggtggc cacgttagag ccgatcagag gtgtccctct cttttgaggt acataaatga
     3421 aggtggaacc acgttgcgac gtcctttcga ggatgtcgca tttttttatt aggatactaa
     3481 ttatggagtt gcaagaatta gtggagcgca gttgggcaat ccgacaagct tatcacgaac
     3541 tggaagttaa gcatcatgat ttcaagtgga cggtagaaga agacctcttg gctttatcta
     3601 atgatattgg aaatttccaa tgactggtga tgacaaagca aggacgctac tatgatgaaa
     3661 caccctacac actggaacaa aaactttcag aaaatatctg gtggctatta gaactttctc
     3721 aacgtttgga tatagacatt ctgacggaaa tggaaaactt cctctctgat aaagaaaagc
     3781 aattgaacgt taggactcgg aagtagtctg ctgataaaaa atcaatgctt agaaactatg
     3841 aaataataaa aaaggagaac atcatgatta acattacttt cccagatggc gctgttcgtg
     3901 aattcgaatc tggcgtaaca acttttgaaa ttgcccaatc tatcggcaat tccctagcta
     3961 aaaaagcctt ggctggtaaa ttcaacggca aactcatcga cactactcgc gctatcactg
     4021 aagatggaag catcgaaatt gtgacacctg atcacgaaga tgcccttcca atcttgcgtc
     4081 actcagcagc tcacttgttc gcccaagcag ctcgtcgtct tttcccagac attcacttgg
     4141 gagttggtcc agccatcgaa gatggtttct actacgatac tgacaacaca gctggtcaaa
     4201 tctctaacga agaccttcct cgtatcgaag aagaaatgca aaaaatcgtc aaagaaaact
     4261 tcccatctat tcgtgaagaa gtgactaaag acgaggcacg tgaaatcttc aaaaatgacc
     4321 cttacaagtt ggaattgatt gaagaacact cagaagacga aggcggtttg actatctatc
     4381 gtcagggtga atatgtagac ctctgccgtg gacctcacgt tccatcaaca ggtcgtatcc
     4441 aaatcttcca ccttctccat gtagctggtg cgtactggcg tggaaacagc gacaacgcta
     4501 tgatgcaacg tatctacggt acagcttggt ttgacaagaa agacttgaaa aactaccttc
     4561 aaatgcgtga agaagctaag gaacgtgacc accgtaaact tggtaaagag cttgacctct
     4621 ttatgatttc acaagaagtg ggacaaggtt tgccattctg gttgccaaat ggtgcgacta
     4681 tccgtcgtga attggaacgc tacatcgtaa acaaagagtt ggcttctggc taccaacacg
     4741 tctacactcc accacttgct tctgttgagc tttacaagac ttctggtcac tgggatcatt
     4801 accaagaaga catgttccca accatggaca tgggtgacgg ggaagaattt gtccttcgtc
     4861 caatgaactg tccgcaccac atccaagttt ttaaacacca tgttcactct taccgtgaat
     4921 tgccaatccg tatcgctgaa atcggtatga tgcaccgtta cgaaaaatct ggtgccctca
     4981 ctggccttca acgtgtacgt gaaatgtcac tcaacgacgg tcacctattc gttactccag
     5041 aacaaatcca agaagaattc caacgtgccc ttcagttgat tatcgatgtt tatgaagact
     5101 tcaacttgac tgactaccgc ttccgcctct ctcttcgtga ccctcaagat actcataagt
     5161 actttgataa cgatgagatg tgggaaaatg cccaaactat gcttcgtgca gctcttgatg
     5221 aaatgggcgt ggactacttt gaagccgaag gtgaagcagc cttctacgga ccaaaattgg
     5281 atatccagat taaaactgcc cttggaaaag aagaaaccct ttctactatc caacttgatt
     5341 tcttgttgcc agaacgcttc gacctcaaat acatcggagc tgatggcgaa gatcaccgtc
     5401 cagtcatgat ccaccgtggg gttatctcaa ctatggaacg cttcacagct atcttgattg
     5461 agaactacaa gggggccttc ccaacatggc tggcaccaca ccaagtaacc ctcatcccag
     5521 tatctaacga aaaacacgtg gactacgctt gggaagtggc caagaaactc cgtgaccgcg
     5581 gtgtccgtgc agacgtagat gagcgcaatg aaaaaatgca gttcaagatc cgtgcttcac
     5641 aaaccagcaa gattccttac caattaattg ttggagacaa agaaatggaa gacgaaacag
     5701 tcaacgttcg tcgctacggc caaaaagaaa cacaaactgt ctcagttgat aattttgttc
     5761 aagctatcct agctgatatc gccaacaaat cacgcgttga gaaataagtg tctagcataa
     5821 aagcctccaa tctggaggct ttttctcatc tatttttact caaggactaa gttcacttga
     5881 gcaaactgaa tccgcactgt cgttcctttt ccgacctcag actcgatacg aatctggtgc
     5941 cccagttctt cagaaatttt cttagataga taaaggccaa gtccagagga ctgctgggtc
     6001 aaacggccat tgtatcctga aaagccacgt tcaaatactc ggaggacatc actgtttttt
     6061 atcccgattc ccgtatcttt gatacaaagc tcttggtcat ccatataaat ctccagacca
     6121 ccttccttgg tgtacttgag actgtttgag atgatttgct caataaccac tagcagccac
     6181 tttttatccg tcacgatttc tttatcaagg tcatgtagat tgacatttaa gcctttttga
     6241 ataaagaaaa gagcatattt acgaattatt tccttgacca agtcctcaat ttgaacctgc
     6301 tttaagacca aatcatcatg gaaactttct aaacgcaggt actgtaaaac taggttggta
     6361 taggagtcga ttttgaaaat ttcctgttct agctgctgct tcagttggcg gtcgaccact
     6421 tctgcaacta agagttgact ggctgcaatg ggggtcttta tctgatggac ccacaaggta
     6481 tagtaatcca gcaaatccgt cagttttctt tctgcttttg acctctgctg atagagttcc
     6541 atctcacgcg cttctaattt ttctgctaaa gctatttcca aaggagactt ggcttccctc
     6601 tctccataga gaagttcctg gcgatagacc tgcgtttcca ccaatatgtc ccaagtgaaa
     6661 aataagatgg ttacaaagca acacaagaag aaaaagtaga ggaagtaaat tcctagactg
     6721 gcaaataaaa actgaaagag taagacaaga aatgccaaag aaagcagata gataaaaaga
     6781 cgactacggg agcgcagata ggctagaaaa aattgtttcc aatcaagcat gcttcaatcc
     6841 gtaccctatt cctttcttgg tctcgataaa tcctaccaat ccctgctcct ccaacttttt
     6901 acgcaaacga gccacattga cagagagggt attatcatca atgaaaaagt cactgttcca
     6961 aagttcccgc atcaggtcgt cacgtgctac gatgttgcct gcatgctcaa ataacacgcg
     7021 taaaatctgg aattcattct tggtcaaatt caagacttgc ccttgataat gtaaatccat
     7081 ggatttggta ttgaggataa caccagcata ttccagcaaa ctctcatcac gcccaaactc
     7141 ataggaacga cgcaacaagc cctgaacctt agctaaaaga acctgctggt caaaaggctt
     7201 ggtcacaaag tcatccgccc ccatattgat tgccatgaca atatccatag cctggtctct
     7261 cgaagaaaga aacatgatag gtaccttgga aatcttgcgg atttcctgac accagtgata
     7321 accattaaac aagggcaaac caatatccat gaggaccaga tgaggttccg actgaacaaa
     7381 tagactcaaa acttccataa agtcttctac caggaccact tcaaatcccc attcagagag
     7441 cattttcccg acctgttgac gaatgacctg atcatcttct attaataaaa tcttgtgcat
     7501 gcgcttctcc ttttctatta ttataacaga tttttccatg ccagatggtc tgaaactgaa
     7561 tttgaaatag cctgttttta gccagtacaa acaggcaatg ctactagcta atttgaggga
     7621 aatttgctaa gataaataaa aagaaaggag ctcttatggc caatattttt gactatctga
     7681 aagatgtcgc atatgattct tattacgacc ttcccttgaa tgagttagac attctaacct
     7741 taatagaaat cacctacctc tcctttgata atctggtctc cacacttcct caacgtcttt
     7801 tagatctagc acctcaggtt ccaagagatc ccaccatgct tactagcaaa aatcgccttc
     7861 aattattaga tgaattggct caacacaagc gcttcaaaaa ttgcaaactc tcccatttta
     7921 tcaacgacat cgaccctgaa ctgcaaaagc aatttgcggc tatgacttat cgtgtcagcc
     7981 tcgataccta tctgattgtc tttcgtggga cagatgacag tatcattggc tggaaggaag
     8041 atttccacct gacctatatg aaggaaattc ctgctcaaaa gcacgccctt cgctatttaa
     8101 agaacttttt tgcccatcat cctaagcaaa aggttattct agctgggcat tccaagggag
     8161 gaaatctcgc tatctatgct gctagccaaa ttgagcaaag tttgcaaaat cagatcacag
     8221 cagtttatac atttgatgca cctggtctcc atcaagaatt gacacagact gcgggttatc
     8281 aaaggataat ggatagaagc aagatattca ttccacaagg ttccattatc ggtatgatgc
     8341 tggaaattcc tgctcaccaa atcatcgttc agagtactgc cctgggtggc atcgcccagc
     8401 acgatacctt tagttggcag attgaggaca agcacttcgt ccaactggat aagaccaaca
     8461 gtgatagcca gcaagtagac acaaccttta aagaatgggt ggccacagtc cctgacgaag
     8521 aacttcagct ctacttcgac ctcttctttg gcactattct tgaggctggt attagctcta
     8581 tcaatgactt ggcttcctta aaggcgcttg aatacattca tcatctcttt gtccaagctc
     8641 aatccctcac tccagaagaa agagaaacct tgggtcgcct tacccagtta ttgattgata
     8701 ctcgttacca ggcatggaaa aatagataat actcttgaca attaaatgta tacaaaacaa
     8761 aagacctaga atacatactt tcatgtgcat tctaagtctt tttaaataga atctaatagt
     8821 caataaaaat caaagagcat tgagagataa tggggcttgg aacgtccctc tcgcttcaac
     8881 aaaatgaccc cattatagat taaaaagatg ccacttagaa aaagcaaaaa aggaagtaag
     8941 acaaaggcaa atacataaaa agctaactga acattctcgt atccattttt ataaaaaagg
     9001 taggatagat aaaaataact tgaaatgagg gataataaaa ataatactgg attccacaaa
     9061 cttctattat ccttccaaaa tgacactata aaggctaata caattcctat aacgagatac
     9121 atttcttact cctttaatag ctacatttta tcataattat ccaaagaaaa aagagggcat
     9181 ttatccctct taatccttca tctgactctc tgcatcggcc acgacttttt ctagactggt
     9241 ttgaccaagt tctgcctcca tagtcaactg aattctctcc aatttttgat ccaaaacatc
     9301 atgaatatga gctcctacag ggcaatttgg attcggattg tcatggaaac tgaagagttg
     9361 acctgtctta ccaagacatt cgaccgcctg ataaacatct aaaagactaa tatccttaag
     9421 gtccttgaca atctctgttc cgcccgttcc acgcgctact gaaatcagct ctgccttctt
     9481 caactgggac aagatctttc tgataatgac aggattgacc ccgacactag cagccagaaa
     9541 atcactggtc accttgcttt ccttcccctc gagggcaatg attatcagca tatgagtcgc
     9601 aatggtaaat ctacttggaa tttgcatcct cttctccttt ttacgaggct accctgcctc
     9661 tactcttctt tttctattat tatacccttt ttagttgtaa tgtcaatcgt taccactttt
     9721 caaccagtcg tctaactccc gatcgcagcc ctctttctga gccaattctc tcaaaaattc
     9781 ctgatgatga gtatggtgga tcccattgac cagactttca tagtaaacct caaaataggg
     9841 aagtctcagg tctttagcca gctgcaattc agctgccaca tcgtaatcta cccgtcggaa
     9901 gtccatatct accaggccct tgtcatcaaa ctccaaaatc atatactggg cccgcaagtc
     9961 cttccgtagc tgagcgtcca aaaagaaagg ttggccaatc gaacccggat tgacaatcaa
    10021 ttgcccacca gtcccgtaac gaagcaactg ctggtgaata tgtccataaa cagcaatatc
    10081 acagggagga tgagtcacca agcggtcaaa ctcctcttgt ttgccagtat gaatcaactc
    10141 tcgcccccag ttcttatcag gcagatgatg gctaattccc accgtcaaat ccccaaactg
    10201 acgatgaatt tgaagaggtt gattgtggag cacttcaatt tcttctaggg aaatttcctc
    10261 taaaacatac tggcactggc gcaagagata gcgttgactg gggcgagtac tgtccaattc
    10321 cttacggaca ccatgccaaa gactgtcttc ccagtttccc aaaactctag ccgtaatcgg
    10381 tagttgatcc aacaagtcca aaatccttct acgtcctgtc cctggcatga gaatatctcc
    10441 caaaagccag tattcatcca ctcctagctg ctgagcatct gccaaaacag cctccaaggc
    10501 ggtggtattt ccatgaatat ctgaaagaag agctattttc gtcatatcca tctcctcgtt
    10561 ttttctcttg caataagtat aacataaaaa gtcacagcta gagaaatcta gctttttttg
    10621 atatactaga taaagatatt agacaagagg aaacgaatga ccccaaacaa agaagactat
    10681 ctaaaatgta tttatgaaat tggcatagac ctgcataaga ttaccaacaa ggaaattgcg
    10741 gctcgcatgc aagtctctcc ccctgccgta actgaaatga tcaaacgaat gaaaagtgaa
    10801 aatctcatcc taaaggacaa ggaatgtggc tatctactga ctgacctcgg tctcaaactg
    10861 gtctctgagc tctatcgtaa gcaccgcttg attgaagttt ttctagttca tcatttagac
    10921 tatacaagtg accagattca cgaggaagct gaggtcttgg aacacactgt ctctgacctg
    10981 ttcgtggaaa gactagataa actgctaggt ttccctaaaa cctgccccca cggaggaact
    11041 attcctgcca agggagaact cctcgttgaa atcaataacc tcccactagc tgatatcaag
    11101 gaagctggcg cctaccgcct gactcgggtg cacgataatt ttgacattct ccattatctg
    11161 gacaagcact cacttcacat cggtgaccag ctccaagtca agcagtttga tggcttcagc
    11221 aataccttca ctatcctcag taacgacgag gatttacaag tgaatatgga cattgcaaaa
    11281 caactctatg tcgagaaaat caactaattt ctcaagtccc ctaccaaccc tgaaagtttt
    11341 attttcaggg ttttatttct atcattttat tttactttca tttggtggta gaatatttga
    11401 aaaaaaaaag aaaggtagtt taacctatga aaaaatcact aaaaattttt gctacatcta
    11461 aatggtttga cctcttcggg gttgctttgg tcgttgggat tgcgattgca tctggttacc
    11521 tcaactcacg cctcgataaa tttgtagact ggggaccatg gacagctctt gtaccctttg
    11581 gattgatttc cgtaaccaac gttgggattt ccatgttgtc cactcgtttc acggggaaat
    11641 taagcaaatg gggaaattac tttagtattg ttaataccat tttgtccggt gctattgatt
    11701 atatccttgg aaataaggcg gccatcatta cctatcccgt caccttcctc atttatacct
    11761 ttgctataaa gaaatggaaa gcttcgcaag aaggcagacc caaccaaatg agccaaaaac
    11821 aggtaaaatt gacggtcatc atcatttcca tcatcgcctt cctctttgcc tttgtgacca
    11881 actatatcgg atatgagggc aagatgaatc tccttgccta cgtaacaact attgcctttg
    11941 cactatccct cattgccaat gctttgaatg cattaaaact aacaactcag tggggctttt
    12001 ggttgattta caatttcgtt cagctgacaa aagctggtat tcaaggaaac ttcgccaata
    12061 tcggtaaata catcttttat atcctcaatg caatcggagc tttatttgtc tggaatgatg
    12121 aagaagttag ataatagaag gaaactcaaa taaagaatac tagccaactt aagagaatct
    12181 cccttctgct atttttatca ttttagttgt aactcccatt cttttggagt agaatgaagt
    12241 tagataaaag agggaggtct tgtcatgaaa aaactctata gaatccattt tatagcaatt
    12301 gcagttattg atttgctact atttgcattt tttatcatta gactcgagac atcttttgag
    12361 tggctcttgc tctctggttt aattttcttc cttgctcaag gactcctgct atttctcttg
    12421 atcgtccgac tcaaacatca attcgctgag atttatcctc aaatcaataa aaagattcgc
    12481 ttctactatt taggggttct caccattgat tttctatttt ttgttctctt agccttcatt
    12541 agttctcagc gtttttcatc tcttatgcca atcatcactg cttgccattc tactttttat
    12601 tatatgacag ctgactacct aagagaaaac tatccagact tttacgacaa acacatctct
    12661 ttatgggagt gtctctaagg aaaaggaggt tttagcatga aaaaaatcat cttcatcaaa
    12721 accattcaac tccttgtcat tgatggaatc atgctagcat ttttgacatt taaaaggggg
    12781 gcttacttgg gactggattt tgatttatag cggttggctc attttctttc atcctgtgct
    12841 attgacctat ctttcaaacc aactttgtga ccactttagt taactctatt cccagattag
    12901 accgagattc tggcgttttg ctttacaaat tctcctatgg gatagcctga tgattctctc
    12961 cttggtgtct ttaagtgata ttccactttt ccttcaggga actctcctca tcctaggaca
    13021 tctcatccct tcctatcgca tctgccaaag cctgaaaaga gacttccccc aagcatatca
    13081 agaaccgatt tctttttgga gtattttatg atagatgaga aagaccaagc cgactgggct
    13141 tggtctttct tatctctttt tagtatctag gataatggta acaggtccgt tattaaccag
    13201 ctcaacctgc atatctgctc caaagatgcc tgtctgaacg ggcacttctt gcgctaattt
    13261 ttgattgaaa gcatcataga agtctgatgc catatcaggt ttagctgccc ctgtaaaggc
    13321 tggacgattg cctttcttag tatccgcaaa gagggtaaac tgagaaatag agaggatttc
    13381 tccttcaata tctttgacag acaggttcat cttgccttct gcgtctgaaa aaatccgcat
    13441 attgaccagt tttctcacag catagtccaa atcttcctct tggtcctctg gtccaacacc
    13501 aaccagcaat aaaagtccct gattgatttt tccctgaatc tggccttcta tactcacttg
    13561 ggctttttta acccgttgga taatgatttt cataatagcc tttctagtaa gagctaggac
    13621 aactagccgt tggtccgttt gacagagtaa acttctggca cactcttaat tttatcgaca
    13681 accgtggtca gtgtagagag gttggcaata ccgaaggaca catggatatt agcaaacttc
    13741 atatccttgg ttggttgggc attgaccgtt gaaatattct tggttgtatt tgaaagaact
    13801 tgcagtacat cgttcaacag tcctgtacgg ttgagaccgt agatatcgat atgggccata
    13861 tactccttat ttgagctaga gtactggtct tcccattcca catcaaggag acgttgctcg
    13921 tagttttctt gggcacgcag gttcatacag tccacacggt gaatagccac accacgaccc
    13981 ttggtaatgt agccaacaat atcgtcacca ggcacggggt tacaacactt agcaatccgc
    14041 actaggagac cagaagcacc ttcaataacc actcccccct catgcttgac cttgagagtt
    14101 tctttatttt caaccttgac ctcgccacct ttgacaagct cctctgcctc agccttggcc
    14161 ttggcacgct cttcctcacg gcgttccttt tcagtcagac ggttaaagac ggtaatcgca
    14221 ccgatttccc caaaaccaat ggccgcaaag agggagtctt ctgtcttgta actggtcttt
    14281 tgcagaactt gatccatgtg gcgcttgtcc ataaatttat ttgccacata gccattttct
    14341 tggaactgag ccatcagcat ctcacgaccc ttgttgacag acaattcctt atcttggttt
    14401 ttaaagaact ggcgaatctt attgcgcgcc ttgctagtct tgaccatatt gagccagtca
    14461 cggctaggtc caaaggagtt cgggttggcg ataatttcaa cctgatcccc tgtctttaac
    14521 ttggttgtca gtggaaccat gcggccattg accttggcac cagttgcttt ttcaccgacc
    14581 ttggtatgga tttcgtaggc aaaatcaatc ggtcctgaat ctttgggaag ggaacggaca
    14641 gctccatctg gggtaaaaac gtaaatctcc tcagccaaat agttttcctt aacagagtcc
    14701 acaaattcct tagcatcatc agcctggtct tggagctcca tcatctcctt gatccagttc
    14761 attccaatag ctgattcctt gctgttaact tgccccttta tacctttctt ataagcccag
    14821 tgagccgcaa ccccgtactc agccacctcg tgcatttcct tggttcgaat ctggaattca
    14881 atcggccctt ttggtccata aacagtcgta tggatagact gataaccatt ggccttgcgg
    14941 ttggcaatat agtctttgaa gcgacctggc atcggtttcc aaaattcatg cacgtaacca
    15001 agcatggcat aaacatcact ttgggtatct aaaatacaac gaatagcaat cagatcatag
    15061 atttcctcaa accgttttct cttgtcctgc attttgcgga aaattgagta aatatgcttg
    15121 ggacgaccat aaatcttccc tttcaagtga cgttctgtcg tgtactcctc taattttgtg
    15181 actacctcat ccaccaaggc ctcacgctcc ctgcgctttt ccttcatcat atgggtaatc
    15241 ttgtaaaact ccgttggatt gagataacgg aaagacaagt cttctaattc ccatttgaca
    15301 ctggaaatcc ccaaacgatg ggcaagtggg gcatagattt ccatggtttc tttggaaata
    15361 cgctcctgct tgtcttttcg aagatgtttc agggtccgca tattgtgcaa gcggtcagac
    15421 agtttgacca aaataacgcg gatgtcctca gacatggcca tgagcatctt gcgatgattt
    15481 tccgctaatt gctcctcgat cgatttgtac tcgaccttgc caagcttggt aactccgtca
    15541 acaatcaccc gcacatcagg accaaactct ctttccaaat cgtccaaggt cgcatctgta
    15601 tcttccacca catcatgcaa gaatccacaa gctactgtta cagcatccag ctttagctta
    15661 gctaaaatac ctgccacttg gatagggtga atgatataag gctcgcctga tttgcgatat
    15721 tgaccactgt ggcattcaac agcatagacc aaggccttat ggacaaaatg aacatcctct
    15781 tccgttaaat attctttggt taaagcgaca acttcttcgc ctgttaaatt cacttctttc
    15841 ggcatctcta ctctccaatt cttcctacca ttttatcact tttttaagaa tatgaaaact
    15901 agattggaac agaataagaa aaaaataatt caaaattgct tgataattct gaattattgg
    15961 cccataatat actacgaagt tagattttaa acttaggtga tagaaggaga gatagaagaa
    16021 cggaaaccat attgtaaccc aaagactttc tgatttcccc aattccattg aagatacgaa
    16081 agataaacgg tggaactcgt atcacataca ctggtacctt gactggattt tggaattaat
    16141 actcaatgaa aatcaaagag caaactagga aactagccgc aggttgctca aagcactgct
    16201 ttgaggttgt agataagact gacaaagtca gtcacatata tacggcaagg cgacgttgac
    16261 gcggtttgaa gagatttttg aagagtataa aaatcctcaa gatactttct tctatccttt
    16321 agtttataag gagaatacct atgaaaaaaa ctgctatttc tatctttgct ctcctaatgt
    16381 taggagtttg ctgcctgttc ctattcagcc agcaaagcta taaaaaactc gttcaatact
    16441 atgctaacga ccagaacctg cccagtagga taacttatag tgaatatagc gacaaatgag
    16501 aagccaacta cggtagcact ctaaacatca cgtctatcaa acaagctaat gacggagttt
    16561 atgcaaccta tgaagggcaa ttgacacctt tccaatattg ataaattgat aaccagcctg
    16621 tcttcatcta gtcatgctgg tttttaagtt cattttaaat ccttacctat tctccctaac
    16681 tgtgctatac ttaatttata ctcaatgaaa atcaaagagc aaactagaaa gctagccgca
    16741 ggctgttcaa agcactgctt tgaggttgca gataaagttg acgcggtttg aagagatttt
    16801 cgaagagtat tagtacattc tttgagattg gagctagtat gaaaatccat aaaaccgtga
    16861 atcctgttgc ctatgaaaat acctattatc tagaaggcga aaagcacctc atcgtcgtcg
    16921 atcctggtag tcattgggaa gccattcgtc agacaatcga gaagatcaac aaaccgatct
    16981 gtgctattct cttgacccac gcccattatg accatatcat gagtctggac ttggttcgcg
    17041 agacgtttgg caatcctcct gtctatatcg cagagagcga agccagctgg ctctacactc
    17101 ctgtcgataa tctctccggt ctccctcgcc acgatgatat ggcagatgtg gtcacaaaac
    17161 ctgcagaaca cacctttgtc tttcacgaag aataccaact agaggaattt cgttttaagg
    17221 ttctaccgac cccagggcac tctatcggtg gtgtttccct agtctttcct gatgctcacc
    17281 tagtcttgac gggagatgct ctattccgcg aaactatcgg acggaccgac cttccgactg
    17341 gtagcatgga gcaactcctt catagtatcc agacccaact cttcacccta ccaaactacg
    17401 atgtctatcc aggacatggt ccagctacta ctatcgctca cgaaaaggcc ttcaatccct
    17461 ttttctagca agatgatgac aatcgaaatt taagtaaact atccagcaaa tctttctatt
    17521 acaaaaggca tcctatcaag gttttcacac gtgattggat gccttttttc tgatgactag
    17581 attttttgta ttaccaaata atcacgcgct cctctggtga acgccacatt ccgtctcctt
    17641 ctttgacatc ataggttgta aagaaatcgt cgaagtttgg tacttgcaca ttgacacgga
    17701 gtttggctgg tgcgtgcaca tcgacgctag ccaaaagttt cataaattct ggtcgacctt
    17761 tcatgcgcca gatgcgaccg aagttgtaga agaactcttc tgctgagaag tctgcttctc
    17821 tcttagctgc ttcaagcgct gctgcgattc ctcccaagtc agccacgttt tctgatacag
    17881 tcaatttacc gttaatggtt gctccataag aatcctgtcc atcaaattgg tcaatgactt
    17941 tttgtgtttt ctccttgaag gcagcatagt cgctctctgt ccaccaatcc ttgaggctac
    18001 cattttcgtc aaaggaagcc ccgttagtat caaaggcgtg ggaaatttca tgggcaatca
    18061 ctgccccaat accaccgtag ttagcagaag atgactgatg caagtcatag aaaggcgcct
    18121 gtaaaatggc cgctggaaag acaatcaggt tcttctgagg attgtagtag gcattgacca
    18181 tatgagcagg catgccccat tccttataat ctacaggctg gttccactta ctccaactgt
    18241 gcttgatttc cacacgcgca aaggctagag cattctcaaa aagactggca gtttcattca
    18301 ctaccttatc cttgtaacgt gcaggcaatt cttctggata gccaatataa ggtttgatca
    18361 cattgagctt cacgatagcc tgtttacagg tttctggagt gagccagtca ttcttaagca
    18421 gacgctcctt atagacatca atcatggttg ccactttttt ctccacatcc gccttggctt
    18481 ctggagagaa cttctcacgg gcgtaccaaa gacccagggc ttgcttgaaa ggttcttgtg
    18541 ctagatgata agctgctttg accttatctt ttgcctctgg aactccagaa agggcacggc
    18601 tgtaggcacc agacaaaaca cggatatcct ctgttaaata gctggttgaa agattgacaa
    18661 cactcaaaat caaggttgct ttaaggagag accaggcttc ctcactgtag aattgctctg
    18721 ctgcttgcca gaaacgttcc tcgtctacaa taaccttgtc tggtaattgc ccaataactg
    18781 ctttgaagaa gtcatccaaa ggtagggcag gcgcgaattt cttgaaatct tcgtaagaat
    18841 atggatgata gagtttagca tattctgaac tttcttcatt agagagcacc actgccgcaa
    18901 ctcggcggtc caattcaagt cttttttcta gcaagtcttc aatttcttca tcagagaaat
    18961 cataagcctt gaggagattt gcgctgcttt ctttccaaag agtcaagagc tcttcgcgct
    19021 gaggatgttc ttctgcatag taggtcgtat ctggcaagat tgtgcttgga gcgctagccc
    19081 atagaacatt gattctagca tccataaagt ctggcgatac accaaaagga aggaagtttg
    19141 gttttcctgc aagctcaaac tctgctagtt tagctataaa atccgcaaaa gtctccaatt
    19201 cttgaaattc tttaaggagt gataagatag gtgtgatacc gtcagcttct ctcttgtcaa
    19261 aatcacgaac taggcggtgg tatttgacaa agttttccaa gatagcatcc tcaggcactt
    19321 cttcacctgc taaccacttg tctgttgtcg ccagcatcaa gtcttcaatt tcctggtcta
    19381 aatcaacaaa acctcctgtt tgagacttat ctgctgggat ttcagctgtc tgttgccatt
    19441 ctccattgat agcatcataa aaatcatctt gataacgtgt catcttgttc tcgctttcat
    19501 ttgtatttgc ctttatctta acaaaaatca ctagggaatg caattagtaa ccatttaact
    19561 attatttttt agctattata aagaatcttt ccatgataaa acgcataatt
//
LOCUS       DAVKGC010000030        18773 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKGC010000030 DAVKGC010000000
VERSION     DAVKGC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.030"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(307..1113)
                     /gene="yidA"
                     /locus_tag="VW049_000867"
     CDS             complement(307..1113)
                     /gene="yidA"
                     /locus_tag="VW049_000867"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9432022.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(1126..2430)
                     /locus_tag="VW049_000868"
     CDS             complement(1126..2430)
                     /locus_tag="VW049_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9432023.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSLIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2502..2879
                     /locus_tag="VW049_000869"
     CDS             2502..2879
                     /locus_tag="VW049_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9432024.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSRYQVDGQKIE
                     LAYQLQNQEGHPFASYQLEITWG"
     gene            2969..3301
                     /locus_tag="VW049_000870"
     CDS             2969..3301
                     /locus_tag="VW049_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9432025.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            3313..4884
                     /gene="ffh"
                     /locus_tag="VW049_000871"
     CDS             3313..4884
                     /gene="ffh"
                     /locus_tag="VW049_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9432026.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5079..6362)
                     /locus_tag="VW049_000872"
     CDS             complement(5079..6362)
                     /locus_tag="VW049_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9432027.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6665..7378)
                     /gene="rsmG"
                     /locus_tag="VW049_000873"
     CDS             complement(6665..7378)
                     /gene="rsmG"
                     /locus_tag="VW049_000873"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9432028.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7472..8032
                     /locus_tag="VW049_000874"
     CDS             7472..8032
                     /locus_tag="VW049_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9432029.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            8034..8933
                     /gene="htpX"
                     /locus_tag="VW049_000875"
     CDS             8034..8933
                     /gene="htpX"
                     /locus_tag="VW049_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9432030.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8977..10536)
                     /locus_tag="VW049_000876"
     CDS             complement(8977..10536)
                     /locus_tag="VW049_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9432031.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10775..11092)
                     /locus_tag="VW049_000877"
     CDS             complement(10775..11092)
                     /locus_tag="VW049_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9432032.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNNQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            11262..11804
                     /locus_tag="VW049_000878"
     CDS             11262..11804
                     /locus_tag="VW049_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9432033.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFASLQGLFDGDE"
     gene            11804..12433
                     /gene="nth"
                     /locus_tag="VW049_000879"
     CDS             11804..12433
                     /gene="nth"
                     /locus_tag="VW049_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9432034.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12644..13165
                     /gene="pyrR"
                     /locus_tag="VW049_000880"
     CDS             12644..13165
                     /gene="pyrR"
                     /locus_tag="VW049_000880"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9432035.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13184..14107
                     /locus_tag="VW049_000881"
     CDS             13184..14107
                     /locus_tag="VW049_000881"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9432036.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14157..15236
                     /gene="carA"
                     /locus_tag="VW049_000882"
     CDS             14157..15236
                     /gene="carA"
                     /locus_tag="VW049_000882"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9432037.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGIGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15548..18724
                     /gene="carB"
                     /locus_tag="VW049_000883"
     CDS             15548..18724
                     /gene="carB"
                     /locus_tag="VW049_000883"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9432038.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEVPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5468 a         3614 c         4221 g         5470 t
ORIGIN      
        1 atatgataaa gtcttatttt tagtgaagta tcctaatttg tataattgat tgtgtgtaga
       61 ttcagggtca atttgaagag agtatttaaa atatcttggg aaattataat ttctccctgt
      121 tttttgagtc caatctatga gtatgagttg cccaggagta aggccacatt ctaattcgtt
      181 taggtaaaaa ttgttacaat cagaatttgt gtctctatta tgattatttt gttttaaatt
      241 aaatagtttg ctaaaaaaat tcattggatt ttattgctaa tcaattctta ttgaaaaatg
      301 atacttttac agtacccatg ttcggatggc atgggcaacg ccggattcgt catttgtttt
      361 ggtgatgtat ttggcgattt ttttgatttc tggatttcca ttttccatga caacggggtt
      421 tccaacgact tccagcatgg cacggtcatt ttcttcatca ccgattgcca tggtttcatc
      481 tttggtcaat ccgagttttt cagccaagtg agtaatggct gaacccttgt ctacattctt
      541 tttaaggagt tcgaggtaga aaggagcaga tttgttgatg gagtagcgct cgtaaaattc
      601 tgctggaatt ttttcaatcg cagcatcgag aatttctggt tcatcgataa acatacattt
      661 aacaatttct ttgccagcca tttcttcagg ggtacggtag aagataggca tgctgacgag
      721 ggttgattcg tgtacagtgt attttccgat attgcgattt gcagtataga taccgtcctt
      781 ggtaatggca tgcatgtgga caccgagctt gcgactgagg aattccatat ccagataatc
      841 ctcataggtc aaggattcgc tgataatctc atggccagta gcagtttctt ggacaagggc
      901 accattgaag gtcaccacat agtcaccttg gtctctcaac tgcaagtcgt ccagaagttt
      961 ggcaacacct gcgatggggc ggccagttgc aatcacgact ttgacaccag cttctttggc
     1021 atcttggatg gcagaaaaga cttcaggagt gatttccttt tggctgttga ctagggttcc
     1081 gtcaatatcg acggcaatta gtttaatact cataaaattc ctcttctagt ttttatttgg
     1141 ggtaaaatga tcgttctcaa tcaagtgtaa aaattgctgg gtaatgcttg cgaagatgct
     1201 gttttggtct aacatttctt tagggaaata gaaacgatta tctccgtggc gactgccagc
     1261 aagggattgg acgataagag acaggctaga gagttcggcc agttctccat ttttttgtaa
     1321 aatctcaatc tgtgtccgtg ggttttcaga ttcgggacga tagatatcat aagggaggtc
     1381 aaagttctta tgaatggcag tgtagtagtc gggatcaaag ccgatatcct caaccaattt
     1441 tctcatgcta gtaagttgat cttggtcctc ttgtgaaaag gtaatggatt taaagacctt
     1501 gcggttgaca aagcgatgcg ataaatctgc aagaatcttg tcaggactgg tcatccaaag
     1561 ctggaagtag gtattcatca cgccatcatc cagagccaga tagtcagtca aggtcacatt
     1621 tttttcgaag aaaggcagga ggtgtggaga agttcgggca aagaaatcct tgtcctcagg
     1681 atagagttcc ttggcgcgct tgagaagatt ctgtaggaga acttccatgg cgcgtgttgc
     1741 tgggtggaaa taaacctgca tgtacatctg gtagcgactg aggacgtagt cttcgatggc
     1801 gtgcatgcca ttgcgctgaa aggcgatacc attttcgaca ggacgaatga ctcggaggat
     1861 gcgagtcagg tcaaattccc cataggatgc tcctgtaaaa taggagtcgc gcaagagata
     1921 gtccatgcga tctgcgtcaa tctgactaga aatgagttgt acgacctgct tgttaggata
     1981 ggtatggtct atgacactgg caactttctc tggaaaatcg ggcgctactt gtagcaggac
     2041 ttggtgaatc tctgtctcag gattttgaat aatctcctga gtaatggctt catggtctgt
     2101 atcaaagaga ttttcaaaag tatgggagta ggcaccatgc ccaaggtcgt gtaggagagc
     2161 agcggtcatg gtcaagagag actcggcagg attccattcc tcaggatatt tttcttcgaa
     2221 aatctctgtg atgcgtcgtg caatttcata gactcctaga cagtgagaga agcgactgtg
     2281 ttctccaccg tggaaggtat aactggaagt tcccagttgt ttgatccggc gcaaacgctg
     2341 aaattctttt gtattaatca agtcatagat gatttgatta ttgacatgga tgtagttgtg
     2401 aactgggtca cggaatactt tttcgttcat atgaccatta tagcaaaatc ctgctctttt
     2461 tggtaaaata gtaggacaag agacaagaaa gaggacctga tgtgaagatt cggatgcgaa
     2521 atacaattca gtttgatgag cagttggaag tgattgacca gctttatgat gtggaagtgc
     2581 atgaaaaagg agattatagc tacctgcttt tctataatga ggaaaaggaa aaagtagtta
     2641 ttaaatttca tggtcaagaa ctggtgatga gccgattttc caatcccaag accattatgc
     2701 gctttttaaa ggatagtgat agtttggcct atattcctac ccctatgggc atgcaggaat
     2761 ttatcattca aacgagccgt tatcaagtag atgggcaaaa gattgagcta gcttatcaac
     2821 tacaaaatca agaggggcat ccctttgcca gctatcaatt ggaaattact tggggctagg
     2881 atctcgtttt tctcaaatct actttctatc ttcaaggatt ggctccctag tgtggtagat
     2941 taggctattt tttggtataa taaaagttat ggaaatcgaa aaaaccaatc gtatgaatgc
     3001 gctctttgaa ttttatgcgg cgcttttgac agataagcaa atgaattata tagagcttta
     3061 ctacgctgat gattacagtc ttgctgagat agctgaggag tttggtgtta gtcgtcaggc
     3121 tgtctatgac aatatcaagc gaacagaaaa gattctggaa gattatgaga tgaaattgca
     3181 catgtactcg gactacattg tccgtagtca gatttttgac caaatcttgg agcgctatcc
     3241 caaggatgat tttctgcagg agcagataga aattttaaca agcattgata atagagaata
     3301 agaggaagaa acatggcatt tgaaagttta acagaacgtt tgcagaacgt ctttaaaaat
     3361 ctccgtaaaa aaggaaaaat ctcagagtct gatgtccaag aggcaaccaa agaaattcgc
     3421 ttggccttgc ttgaggccga cgttgctttg cctgttgtaa aggactttat caagaaagtt
     3481 cgtgagcgtg cagtcgggca tgaggtcatt gatacactta atcctgcgca acagattatt
     3541 aaaatcgttg atgaggaact gacagccgtt ttaggttctg atacggcaga aattatcaag
     3601 tcacctaaga ttccaaccat catcatgatg gttggtttac aaggggctgg taaaacaacc
     3661 tttgctggta aattggccaa caaactcaag aaagaagaaa atgctcgtcc tttgatgatt
     3721 gcggcggata tttatcgtcc agctgccatt gaccagctta agaccttggg acaacagatt
     3781 gatgtgcctg tctttgcact tggaacagaa gtaccagctg ttgagattgt acgtcaaggt
     3841 ttggagcaag cccaaactaa tcataacgac tatgtcttga ttgatactgc gggtcgtttg
     3901 caaattgatg agctcctcat gaatgagctt cgtgatgtga aagcattggc tcaaccaaat
     3961 gaaattctcc tcgtcgttga tgccatgatt ggtcaggaag cagccaatgt tgcgcgtgag
     4021 tttaatgctc agttggaagt gactggggtc atccttacca agattgatgg cgatactcgt
     4081 ggtggtgcgg ctctatcggt tcgtcacatc actggaaaac caatcaagtt cactggtaca
     4141 ggtgaaaaaa ttacagatat cgaaaccttc cacccagacc gtatgtctag ccgtatcctt
     4201 ggcatggggg atatgctcac tttgattgag aaagcttctc aggaatacga tgaacaaaaa
     4261 gcccttgaaa tggctgagaa gatgcgcgaa aacacctttg attttaatga tttcatcgat
     4321 caattagatc aggtgcaaaa tatggggccg atggaagact tgctcaagat gattccaggt
     4381 atggccaaca atccagcact tcaaaacatg aaggtggatg aacgccagat tgctcgtaaa
     4441 cgtgccattg tgtcttcgat gacacctgaa gaacgtgaaa acccagattt gttaaatcca
     4501 agccgtcgcc gtcgtattgc tgctggttct ggaaatacat tcgtcgaagt caataaattt
     4561 atcaaggact ttaaccaggc taaacagctc atgcagggtg ttatgtctgg ggatatgaat
     4621 aaaatgatga agcaaatggg gattaatcca aataaccttc ctaaaaatat gccaaatatg
     4681 ggaggaatgg atatgtctgc ccttgaagga atgatgggac aaggcggtat gcctgactta
     4741 tcagctctcg gaggagcagg aatgccagat atgagccaga tgtttggtgg cggtttgaaa
     4801 ggtaaaattg gtgaatttgc catgaaacag tccatgaaac gtatggctaa caaaatgaag
     4861 aaagcgaaga agaaacgcaa gtaagacaag agattgatgc aaatctcatt tgatgagaaa
     4921 ataaagtaaa aatttctatg ataaaacgta cagtatcaag gttttagaat atctgatatt
     4981 atacgttttt tgattttaat aattttgtta agacactttt attatgatat acgaaaaatc
     5041 cactcagctg tctgagtgga ttaggtgtat ttagactctt agtctttatt ttcgtatggc
     5101 aagatcaagt tcaagatgat tcctgtcatg gctgaaaggg cagtacctga aagtgtaact
     5161 ggaccaagtt taaggatagc tcctccaagt ccaagaacca acatagcact tgcgatgatg
     5221 aggtttcgca tttgagcgaa atcaacacgt tctttaatca agactttcaa accattgctg
     5281 gcgataaccc catagagaag gattgacata ccaccaagta cagcgtttgg aatagttgaa
     5341 atcaaggcag tgaatttacc aaggaagctg agggcaatcg cgatgaaggc agcgttacgg
     5401 ataactgaga cagaagcgat acgagtcata ccgataaccc ctgtattttc tccgtaagtt
     5461 gtattggctg gtccaccaag gaaggcagaa acagaagttg cgataccgtc accaagaaga
     5521 gtacggtgaa gacctggttc ttttaagaat tgacgaccac agatttgacc caaaacagta
     5581 tggtctccga tatgttcaga aattgttacg atagcgattg gcaagatagc gatggcttct
     5641 ggaccaaagt aaagattgta ctctttaaag gcaccacctg tgctaaatgg caagtagaaa
     5701 ccaggaattt cgaaccagtt ggctttaaga actggtgtaa agtcaaccaa gccaagagtt
     5761 agtgcgaaaa ggtaaccacc gataatggca aagaggaatg gaatgattcg taggaagcct
     5821 tttccttttg tattgataaa ggcagcaatt aggaaagtaa caacggctac cagagcattt
     5881 ttccaatttc cgtctgctac aagacctgca ttggtaacag ctgaacctgc aagtccaaga
     5941 ccgataacga tgatcatagg accgataatg attggtggca agagtttatc aatccatttt
     6001 gttcctacaa atcggatgct ggtagcaaca aggacataga ccaaaccagt caagataacc
     6061 cctgtttggg cagcagatac atcccccccc atttctttca tagccagtga catagctgtg
     6121 ataaaggcaa atgaagaacc tagataaact ggaactttaa aaccagtagc aatcatgtag
     6181 atgagtgttc caacacctga agcaaaaagg gcaacagata caggcattcc caaaatcaat
     6241 ggtaccaaga tggtcgcacc aaacatggcg aaaacgtgtt ggaagctaag gagaattcct
     6301 tttccatccg aaggacgttg gtcaacgtct agtaacaaat caacagttga ttcttgtttc
     6361 atataaacct cacttttggg catcaaaaaa gagcccatta ttttacagca ataggccctc
     6421 agagtaagac ttctttaaaa tttagatttt aaagagtttc aaatatttct gcgtcttcgt
     6481 cacctcacgg gatgacatta aaaacctttg cttaggatag tatagcagaa aaaaatgatt
     6541 ttgtaaatca ttttttcccg agcctagaaa taaaagagcg aggctgattt tgtaaatcat
     6601 tttttcccga gcctagaaat aaaagagcga ggctgatttt gtaaacattt gtttactaaa
     6661 aaatttaaag tgggcgttta tttggcatac cagccttacg cggatattta tttggtgttt
     6721 cttttttctt ttctaccact gtgatatagc gcggatctct attcggtagg gcgtagctga
     6781 gattgtcttc gaccttacta aaaaggagat tgagggcatt cttagcttct aataattcct
     6841 caggcgcatt gctggccttg agtgccaata gttttccacc aaccttaagg tagggaatgg
     6901 tcaattcaga taggacctgc atacgggcaa ccgcacgagc tgttacaaaa tcatattgag
     6961 cacggaagtt cttgtcttgg gcaaaatctt cggcacgtcc gtggtagaaa tgaactccgt
     7021 tcaaatccag ttcttgagcc aagagttgta ggaagttgat gcgcttattg agtgaatcaa
     7081 taatggtcac atctaactcc ggatagagaa ttttcattgg tagactagga aatcctgccc
     7141 cagccccgat atcaagaagt ttgatagttt cattgggaat caaaccttga agaatgggtg
     7201 caatcgaatc gtaaaaatgt ttgagataaa cttcttcctt gtccgtaatc gccgtcaaat
     7261 taatcttctc attccactcg accaagagct caaaataacg ttcaaattgt tctttttgct
     7321 ggtccgaaag tggaagattc tgctcggcaa gcaagttgta aaatgttttt ggtttcatag
     7381 ttatttcctt ctttagtatc atcttatttt accacaatca tagaaaattt gatatactgg
     7441 tactaatcta aaagcagaaa ggacttatat tatgacttgg attattcttg gagttatcgc
     7501 tcttattgtt atttttgtga ttgttagcta taacggtttg gttaaaaatc gtatgcaaac
     7561 caaggaggct tggagtcaga ttgatgttca gttgaaacgt cgaaatgacc tcttgccaaa
     7621 cttgattgag actgtaaaag gttatgccaa atatgaaggt tctacccttg aaaaggtggc
     7681 agaactacgt aaccaagtgg cggcagcgac ttcaccagca gaagctatga aagccagtga
     7741 tgccctcact cgtcaggttt caggtatttt tgcagttgca gaaagctatc cagatttgaa
     7801 agctagtgct aactttgtta aattgcaaga ggagttgaca aacacagaaa ataaaatttc
     7861 ttactctcgt caactctata acagtgttgt cagcaactac aatgtaaaat tagaaacttt
     7921 cccgagcaat attatcgctg gaatgtttgg atttaaagcg gcagatttcc ttcaaacacc
     7981 tgaagaggaa aagtcggttc ctaaagttga ttttagcggt ttaggtgact aagatgttgt
     8041 ttgatcaaat tgcaagcaat aaacgaaaaa cctggatttt gttgctggta tttttcctac
     8101 tcttagctct tgttggttat gcggttggtt atctctttat aagatctgga cttggtggtt
     8161 tggttattgc actgattatc ggctttatct acgctttgtc tatgattttt caatcgacag
     8221 agattgtcat gtccatgaat ggagcgcgtg aggtggatga gcaaacggca ccagacctct
     8281 accatgtagt ggaagatatg gctctggtcg ctcagattcc tatgccccgt gttttcatca
     8341 ttgatgatcc agccttaaat gcctttgcga caggttctaa tcctcaaaat gcggctgttg
     8401 ctgcgacttc aggtctacta gctatcatga atcgtgaaga actagaagct gttatgggac
     8461 atgaagtcag tcatattcgt aattatgata tccgtatttc gactattgca gttgcccttg
     8521 ctagtgctat caccatgctt tctagtatgg caggtcgtat gatgtggtgg ggtggagcag
     8581 gtcgcagacg aagtgatgat gaccgagatg gaaatggtct tgaaatcatt atgctagtgg
     8641 tttccctact agctattgta ctggcacctc tcgctgcaac cttggttcag ctcgctattt
     8701 ctcgtcagag ggaatttctg gcagatgcat ctagtgtcga gctgactcgc aatccccagg
     8761 gaatgattaa tgccctagat aagttggaca atagcaaacc tatgagtcgc cacgtcgatg
     8821 atgctagcag tgccctttat atcaatgatc ctaagaaagg tggggggttc caaaaactct
     8881 tttataccca cccacctatc tcagaacgga ttgaacgttt aaaacagatg taaaagaatc
     8941 cagaggtcgt ctctggattt ttgctatgtt gcaaatttat aaatcttcta aattaactag
     9001 cttcaaaccg tgttctgtca tgcgatagat gatcgagcag acttctttta agaaacgacg
     9061 gtcatgcgaa acagtgatga gaccgcctgg ataggtagca aagagttttc tgatttgggg
     9121 ttgagaagtg ggagaaaagt ttcgtgtggg ttcatccagc aggagaaagt ttggtttgcg
     9181 caggactaaa tccaaaagca ggagttttcc ctgttgtccg ccagataagg agcgaatttg
     9241 atgctgcatt tctggataac tgaaattgag actagctagg tgagattgga ttttctgtag
     9301 ttcctctttt tccccagttt tactgagata ggctattggg gataaatcca attgcagttt
     9361 tttgtggtaa tcttgtggca taaaaccaag tgaaatctct cttttatcat tcagaagtct
     9421 ctgtaacttg gctaacagag ttgatttccc aacaccattt ggcccgataa taccgatttt
     9481 ttcttggcca cggacagtta gttgtagttt ttgaaccaaa actcggtcgt caatggacaa
     9541 attttctttt tccagttgga ctaagacttt agaagctggt aatggttgga tgtctgaaaa
     9601 gaaaagttgg atttgttcct cttcaagtgg cttttgagtc atggactgag ctgccttttc
     9661 gtagcgtttt tcttgtgaga ggacagtttt catcttttta gccaataggc gaccggcagt
     9721 actatctttg gtagctcgaa gcgcagtttc tacattttgc ttaactctcc gatgtttttc
     9781 catggtttta tcgtaggctc tttggttgtt agcagcttgc tgactttgtt tggcaaaatt
     9841 agcctttctc tgctcactat agctatcata gtctaaatgc tctactagcg tttccgcttc
     9901 tttacggtgt ttgaccagtc gcaagtgaac aatagtgtct gccgtttcag aaagaaagtc
     9961 ttcatcatgg gaaatgaaaa taacggtttg cctggtcttt tgaatctggc cttttagcca
    10021 atcaactgtc tcaaggtcta ggtcatttga aggttcatct aaaaatagaa tctcaaaggg
    10081 tttggctaac tcatggataa gctgaatttt caaagcttcg ccccctgata gattgccaat
    10141 ctcttggtca cttgcgaaac gattgctatc aaaatgcaat tcctccgcca aacgatagag
    10201 gatactgtag tctaaatcaa tagaatctaa aaagaagtag tcgtgtaaag ttttcttttt
    10261 taggtcctca gggacttttt gaggaatgta ggccagtgac tgatagtcag attggatgtt
    10321 tcccttgata gtgaaatcag acaaagcttc ccccattaaa attttaagta aggttgattt
    10381 gccatttcct tcttcaccaa taatagcaac cttttccccg tcttggatgg tcatggttag
    10441 gtcagataca agatctcgta aatctttgtt ttgtgtgatg gttagatgat tgatttttat
    10501 catatgattt ccctttctag aatgttcata gtgcataaca aaaagctcta ctttacctag
    10561 tagagcccaa agtctatgtc aaaaaaactc tattaacatg ccagaaaaag agcataaaag
    10621 agcacacaaa aagagacaaa aacaagatct actaaataaa ggttctttat ttgatagact
    10681 tagttgttca tgtctccctt acctccgagt attagtatct ttatactata cctttaagga
    10741 aattttgtca acagaaaact ggaaattcta tgctctaaaa tccaaaaaca ggtccataga
    10801 gacttagtac gtgtttgaca tgctcgtaat ggattttatc atagtttcgc caagcagtgc
    10861 gtgcttgatt gtttagtttt aggctaccca gtccaatcag aggactggtg ggttggtaaa
    10921 atttctcaag atcaattaag atactgttgt tcaacttttc cgtttttttg agctcttgca
    10981 tagtagtgtt tagcagctct tgtagtcgaa tatcatctgc cgtaccctgt ttacagtttt
    11041 ggtagctttt atttaacttg gataggagtt gaatgagttt cttgtcttcc atgtgagcat
    11101 cctccttgat ctgatgtact ctttccctat agtatagcac taaaaaacaa aactatcatg
    11161 gtaaatatgt taaaattttg aattttaatt acaaagtttg gtgtgctgat ttgtagcctt
    11221 ttcatggtat aatcaagaga gtaaatcttt atggaggaag tatgaaatta aatattcaag
    11281 aaattcgtaa gcagtctgaa ggtttgaact ttgaacaaac gttagattta gttgatgacc
    11341 tgcgtgcacg taatcaagaa attttagatg taaaagatat ccttgcagtt gggaaagtac
    11401 aatatgaaga ccgtatgtat ttcttagatt atcaactatc ttataccatt gttcttgctt
    11461 cgagtcgcag tatggagcca gttgagttag ttgaatctta tccagtcacg gaagttttca
    11521 tggaaggcgc aactaaccat ctaaatcaag aaattttaga tgatgacttg gtcttgccca
    11581 tcgaaaatgg ggcgcttgac cttgctgaga gtgtatcaga caatatcctg ctaaacattc
    11641 ctatcaaggt cttgacggct gaagaagaag ctggtcaagg atttgtctca ggaaatgact
    11701 ggcaaatcat gacagaggaa gaataccaag ctcaacaagc agtaaagaaa gaagaaaaca
    11761 gtccttttgc tagcttacaa ggactatttg acggagatga ataatggtct tgtcaaaaaa
    11821 acgagcacga aaggtgctag aagaaatcat tgccctcttc ccagatgcca agcctagtct
    11881 tgattttacc aatcattttg aactcctggt tgcggtcatg ttatcagccc agacgacaga
    11941 tgcagcggta aataaggcca caccaggtct ctttgttgcc tttccaacac cccaagctat
    12001 gtctgtagcg acagagagtg agattgcttc acatatttct cgcttgggat tgtatcggaa
    12061 taaagctaaa ttccttaaaa aatgtgccca acagttacta gacgattttg atggtcaagt
    12121 ccctcagaca cgtgaagaat tggagagttt ggcaggtgtt ggtcgcaaga cagccaatgt
    12181 tgtcatgagt gtaggatttg ggattccagc ctttgcagtg gatactcatg tggagcgtat
    12241 ttgcaaacac cacgatattg tcaaaaaatc agcgacgcca cttgaggtgg aaaagcgggt
    12301 catggatatc ttgccgcctg agcagtggtt agctgcccat caggccatga tttattttgg
    12361 aagagccatt tgccatccaa aaaatccaga gtgtgatcag tacccacaat tatatgattt
    12421 tagcaatttg taagatgcta cattgaaact ttttgcttga ttttaagcat gctttgtgct
    12481 atagtaaatg ataactaaat attcctttaa acctgtcccg tgaggcaggc aaggagcgcg
    12541 ataaagtaga agggtgaagt ctatactgtg tgcagtaggg ctcgcttggc tataaatttc
    12601 aagtctcctt gtttaaggag acttttcttt ttggaggttt gtcatgaaaa caaaagaagt
    12661 tgtagacgaa ttgaccgtca aacgagcgat tacgcgtatt acttatgaga ttatcgaacg
    12721 caacaaagat ttgaataaga tcgtcttggc tggtattaaa actcgtggtg tctttattgc
    12781 ccaccgaatc caagaacgtt tgaagcagtt agaaaatctt tcagttcctg ttgtggaatt
    12841 ggatactaaa cctttccgtg atgatgttaa aagtggagaa gatacttctt tggtttctgt
    12901 cgatgtgaca gaccgcgaag ttatcttggt ggatgatgtg ctctatacag gtcgtaccat
    12961 ccgtgctgct attgataata ttgtcggtca tggtcgtcct gcgcgtgtga gtttagcagt
    13021 tctagtcgat cgtggacata gagaattgcc aatccgtcca gattacgttg gaaaaaatat
    13081 cccaaccagt cgttctgaag aaatcatcgt agagatgaca gaacttgatg accaagacag
    13141 agttctgatt actgaagaag cttagaaagt taaaggagta gccatgtcag aaaatcaaca
    13201 agcattgaac catgtggtgt ccatggaaga cctcactgtc gatcaagtaa tgaaattgat
    13261 caagcgagga attgagttta aaaatggagc ccagcttccc tatgaagacc atccgattgt
    13321 ttccaatctc ttctttgagg attctacacg gacacataag tcctttgaag tcgcagagat
    13381 taaacttgga ttggaacgac ttgactttga tgtgaagact agctcggtta ataagggtga
    13441 gacactttat gacaccattt tgactctgtc tgctttagga gtggatgtct gtgtgattcg
    13501 ccatccagag gtcgactact atagagaatt gattgctagt ccaacgatta cgacttccat
    13561 catcaatggt ggagatggtt cgggccaaca ccctagccag agcttgcttg atttgatgac
    13621 catttatgag gaatttggcc actttgaggg tcttaaagtt gctattgcag gtgacttgga
    13681 ccactcacgc gttgccaaat ccaatatgca gattttgaaa cgcttgggag ctgaactctt
    13741 tttcgctgga cctgaggaat ggagaagtca agagtttgca gactatggac agtttgtaac
    13801 cattgatgaa atcattgatc aggttgatgt catgatgttt ctccgtgtgc aacacgaacg
    13861 ccatgatagt ggagctgtat tttcaaaaga agactaccat gcccaacatg gcttgactca
    13921 agaacgttac gatcgtttga aagaaacagc aatcctcatg cacccagctc caatcaatcg
    13981 tgatgtagaa atagcagacc acttggttga agcaccaaaa tcacggattg tccaacaaat
    14041 gaccaatggt gtctttgttc gaatggcaat cttagaatcc gtactagcga gtagaaacgc
    14101 caactaaaac acaagacgtt aaaagacgcc agtgagcgtc cacgagaggt gaaaatatga
    14161 caaaaagaat tctagtatta gaagatggca cagtttttga aggcaaggcc ttcggagcag
    14221 atattgatgt aacaggcgaa atcgtcttta atacagggat gaccggctac caagaatcca
    14281 ttacagacca gtcttataat ggacaaatct tgacctttac ttatcctttg gtaggaaatt
    14341 atggtattaa tcgtgatgat tatgaatcca ttattccaac ttgtaaggga gtcgttgttt
    14401 ttgaagaagc gcgtagagct agcaactggc gcaaccaaat gaccttggat gaatttttga
    14461 aagccaagaa aattccaggg atttcaggaa ttgatacgcg tgctcttacc aagattatcc
    14521 gtaagcatgg tactatgcgt gcaaccttga cccatgttgg ggacagtatg gaccatgtga
    14581 cggaccagct ccaagcaaca gttttaccga cagacaatat caaacaggtt tctactaaaa
    14641 cgtcttatcc agctccagga attggtttga gcgtggtgct agtggacttt ggtctcaagc
    14701 actcaatcct acgtgaactt tctaaacgca actgtaacgt gacagttgtt ccttattcga
    14761 caacggctga agaaattctc catctcaatc ctgacggagt tatgttgtca aatggtccag
    14821 gtaacccaga agacgttcca caagcactcg acatgattcg cggtgtacaa ggaaaaattc
    14881 caatctttgg tatttgtatg ggacaccaac tctttgcaat ggcaaacggg gctaagacct
    14941 ataagatgaa gtttggccac cgtggattca accatgcggt acgtgaaatc gcaacaggac
    15001 gagtagactt caccagccag aaccatggtt atgcagtcag ccgcgaggat ttgccagagc
    15061 acttgattat tacccacgaa gaaatcaatg acaagtcagt ggaaggtgtg cgccacagat
    15121 accaacctgc tttctctgtt caataccacc cagatgcagc tcctgggcca cacgacgcta
    15181 gctacctatt tgacgagttt atcgagatga tggaagtttt taaacaatca aactaagaac
    15241 gagtaaaagc tcgtcggaga atttatgtaa ctgaacacgg acggaaagct cagaatgtag
    15301 agaaaccaac ttggattgtc aatccttcct tgatttccta aaattcaatc gctttctatt
    15361 cgtcctttgt atcttattta atgtcgccct gagaggcgac gaatagaaag gagaagatac
    15421 attgttcgcg gaagtgaaca cgccctaaga actgtgtgaa aaagataaac atcgtcttga
    15481 cgctgaaggc gtctgcgccg agtttcctat tttcactgtg ttctttacgg gctttgtatc
    15541 ataaactatg cctaaacgta ctgatattca aaaaattatg gtgattggtt ctggtccgat
    15601 tattattggt caggctgctg agtttgacta cgctgggact caggcttgct tgtcgttgaa
    15661 agaggaaggt tatgaggttg ttttggttaa ctcaaatcct gcaaccatca tgacggacaa
    15721 ggaaattgct gacaaggttt acattgaacc gattacactt gagtttgtga cacgtattct
    15781 tcgtaaggaa ggtccagatg ccttgctacc aacactcggt ggtcagacag gtctcaacat
    15841 ggccatggaa ttatctaaaa atggtatcct agacgagctt ggcgttgaac ttctgggtac
    15901 caaattatct gccattgacc aagcggagga ccgtgacctc tttaaacaat tgatggaaga
    15961 gcttgagcaa cccattccag aatctgaaat tgttaacaca gtagaagaag cagttgcctt
    16021 tgcagcgaca attggctacc cagtcatcgt tcgtccagcc tttacccttg gtggtactgg
    16081 tggtggtatg tgtgccaacg aaaaagaatt gcgtgaaatc actgaaaatg gtttgaaatt
    16141 gtcacctgtt acccaatgtt tgattgagcg ttcgattgcc ggtttcaagg aaattgaata
    16201 cgaagtgatg cgtgactcag ctgacaatgc cttggttgtt tgtaacatgg aaaactttga
    16261 cccagttggg attcacacag gggattccat tgtatttgcc cctgcgcaaa ccatgtcaga
    16321 ctatgaaaac caaatgcttc gtgatgcgag cttgagcatt attcgcgccc tcaagattga
    16381 aggtggatgt aatgttcagc tagctcttga tcctaacagc ttcaagtatt atgttatcga
    16441 agtaaaccct cgtgtatcac gttcgtcagc ccttgcttct aaagcgacag gatatccgat
    16501 tgctaagttg gctgccaaga ttgcagtagg tttgaccttg gatgaggtta taaacccagt
    16561 tacaggttca acctatgcca tgtttgaacc agcccttgac tacgtagttg ctaagattcc
    16621 acgtttccca tttgacaagt ttgaaaaggg tgaacgccgt ctcggtacac aaatgaaggc
    16681 gactggagaa gtcatggcaa tcggtcgtaa catcgaggaa tctcttctca aggcctgccg
    16741 atcccttgaa attggggtgc accacaatga agtacctgaa cttgcagcag tttctgatga
    16801 tgccttgatt gaaaaggttg tcaaagccca agatgaccgt ctcttctacg tatcagaagc
    16861 aattcgtcgt ggctacacac ctgaagaaat tgcagagctt acaaaaattg atatcttcta
    16921 tcttgataaa ctcttgcata tctttgaaat tgagcaagaa ttgggtgctc atccacaaga
    16981 tctagaagtt ttgaaaacag ccaaacttaa tggtttttca gaccgtaaga ttgctgaact
    17041 ctggggaacg actgatgata aagttcgcca acttcgtttg gaaaacaaga ttgtcccagt
    17101 ctataagatg gtagatactt gtgcggcaga gttcgactcc gaaacgccat atttctattc
    17161 aacctatggt tgggaaaatg aatctatcag atctgataaa gaatctgtac ttgtcctagg
    17221 ttcaggtcca atccgtatcg gtcaaggggt tgagtttgac tacgcaaccg ttcactctgt
    17281 taaggctatc caggcagctg gttatgaagc tatcatcatg aactcaaacc cagagaccgt
    17341 ttcaacagac ttctctgtat cagataagct ttactttgag ccattgacat tcgaagatgt
    17401 tatgaacgtc attgacttgg aacaaccaaa gggagttatc gttcagttcg gtggtcaaac
    17461 agccatcaac cttgcggagc cattggcaaa agcaggtgtg accatccttg gtacacaggt
    17521 tgctgaccta gaccgtgccg aagaccgtga cctcttcgag caagctctca aagaattgga
    17581 tattccacag ccaccaggac aaacggctac caatgaagaa gaagcagctc ttgcagctcg
    17641 taagattggc ttcccagttc ttgtccgccc atcttatgta cttggtggac gtgccatgga
    17701 aatcgttgaa aacgaagaag accttcgttc ttacatgcga actgctgtta aggctagtcc
    17761 agaccatcca gttcttgtcg actcttatat cgttgggcaa gagtgcgaag ttgatgccat
    17821 ttcagatggt aaaaatgtac tcattcctgg tatcatggag catatcgaac gtgctggtgt
    17881 ccactcaggt gactcaatgg ctgtttaccc accacaaacc ttgtcgcaaa aggttcagga
    17941 gaccatcgca gactacacca aacgcttagc aatcggtctt cactgccttg gaatgatgaa
    18001 catccagttt gttatcaagg atgaaaaagt ctacgttatt gaggtcaatc cacgtgccag
    18061 ccgtaccgtt ccattcctat ctaaggtaac caatattcct atggctcaag tagcgaccaa
    18121 gctcattctt ggtcaaagtt tatcagaact tggctaccaa aatggacttt accctgaaag
    18181 cacccgtgtt catatcaagg cacctgtctt ctcctttacc aaactagcta aggtagacag
    18241 cttgctcggt cctgaaatga agtcaacagg tgaagttatg ggttctgatg ctactttgga
    18301 aaaagctctc tataaagcct ttgaagcttc ttatctacac ttgccaactt ttggtaatgt
    18361 cgtctttacc atcgctgatg atgccaaaga agaagccttg aacttggctc gtcgtttcca
    18421 aaacattggt tatggaatcc ttgcgacaga agggacagca gccttctttg ctagtcatgg
    18481 cttgcaagcc caacctgttg gtaagattgg tgatgacgat aaggatatcc caagttttgt
    18541 acgcaaagga agaattcaag ctatcattaa cacagtagga acaaaacgaa ctgctgacga
    18601 agatggtgag caaattcgtc gttcagccat tgaacacggg gtgcccctct tcacagcgct
    18661 agatacagct aatgccatgc ttaaggtact tgaaagtcgt agctttgtta cagaagcgat
    18721 ttaaatagaa aaataaggta tagcagactg aatttagaac agtacacagg gat
//
LOCUS       DAVKGC010000031        17968 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKGC010000031 DAVKGC010000000
VERSION     DAVKGC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17968
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.031"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            355..1401
                     /gene="pheS"
                     /locus_tag="VW049_000884"
     CDS             355..1401
                     /gene="pheS"
                     /locus_tag="VW049_000884"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9432039.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1401..1910
                     /locus_tag="VW049_000885"
     CDS             1401..1910
                     /locus_tag="VW049_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9432040.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTVSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1987..4392
                     /locus_tag="VW049_000886"
     CDS             1987..4392
                     /locus_tag="VW049_000886"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9432041.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVESVESPAADLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVIIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDN
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VRVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4460..5461)
                     /locus_tag="VW049_000887"
     CDS             complement(4460..5461)
                     /locus_tag="VW049_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9432042.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5617..6106
                     /locus_tag="VW049_000888"
                     /pseudo
     CDS             5617..6106
                     /locus_tag="VW049_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6191..6457
                     /locus_tag="VW049_000889"
     CDS             6191..6457
                     /locus_tag="VW049_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9432043.1"
                     /translation="MEGCLSELFSKEEILESDMRVAIMSELIEARNKQGISQKKLEEV
                     SGVSQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEQGKS"
     gene            6717..8966
                     /gene="metE"
                     /locus_tag="VW049_000890"
     CDS             6717..8966
                     /gene="metE"
                     /locus_tag="VW049_000890"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9432044.1"
                     /translation="MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVSAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLTVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEVILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9030..9896
                     /gene="metF"
                     /locus_tag="VW049_000891"
     CDS             9030..9896
                     /gene="metF"
                     /locus_tag="VW049_000891"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9432045.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVTETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIELIKERAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDIAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10581..12794
                     /gene="pnp"
                     /locus_tag="VW049_000892"
     CDS             10581..12794
                     /gene="pnp"
                     /locus_tag="VW049_000892"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9432046.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            12810..13427
                     /gene="cysE"
                     /locus_tag="VW049_000893"
     CDS             12810..13427
                     /gene="cysE"
                     /locus_tag="VW049_000893"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9432047.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13439..14323
                     /locus_tag="VW049_000894"
     CDS             13439..14323
                     /locus_tag="VW049_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9432048.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14405..15748
                     /locus_tag="VW049_000895"
     CDS             14405..15748
                     /locus_tag="VW049_000895"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9432049.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15741..16127
                     /locus_tag="VW049_000896"
     CDS             15741..16127
                     /locus_tag="VW049_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9432050.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16131..17015
                     /locus_tag="VW049_000897"
     CDS             16131..17015
                     /locus_tag="VW049_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9432051.1"
                     /translation="MREKELQDWFPDARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVTDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQIGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            17152..17751
                     /locus_tag="VW049_000898"
     CDS             17152..17751
                     /locus_tag="VW049_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432052.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
BASE COUNT         5397 a         3477 c         4063 g         5031 t
ORIGIN      
        1 tttactttcc caatcgattt gttttcatct tatttcaatc cgctataaag ttagataaga
       61 ataagctgac aagcagaagt aagataggtc ttgcaagaat ggtaaaaatc tgataaacta
      121 agtagtaatt gaataggaat ctgaaagact agtagcccaa gggaagtata tcagggagga
      181 gagccgtgac tgcaagctct ctatatgaaa gctgggtgaa ttcacttgcg catggattta
      241 gaaatgaagg tctgacttgt cagatgaaaa cggatggtac cgcgtgtcaa cgctccgagt
      301 ggagtttttg gcatgtggtt ttctttttat ctacgagaga ctgatggagg aaatatgtca
      361 actattgaag aacaattaaa agcgcttcgc gaagaaacgc tggctagctt gaagcagatt
      421 actgctggaa atgaaaaaga gatgcaagat ttgcgtgtct ctgtccttgg taaaaagggt
      481 tcgctcactg aaatcctcaa agggatgaaa gatgtttctg ctgagatgcg tccaatcatc
      541 gggaaacacg tcaatgaagc tcgtgatgtc ttgactgctg cttttgaaga aacagctaag
      601 ctcttggaag aaaagaaagt cgcggctcaa ctggctagcg agagtatcga tgtgacgctt
      661 ccaggtcgtc cagttgcgac tggtcaccgt cacgttttga cacaaaccag tgaagaaatc
      721 gaagatatct tcatcggtat gggttatcaa gtcgtggatg gttttgaagt ggagcaagac
      781 tactataact ttgaacgtat gaaccttcca aaagaccacc cagctcgtga tatgcaggat
      841 actttctata tcactgaaga aatcttgctc cgtacccaca cgtctccagt tcaggcgcgt
      901 gctatggatg cccatgattt ttctaaaggt cctttgaaga tgatctcgcc agggcgtgtc
      961 ttccgtcgcg atacggacga tgcgacccac agtcaccaat tccaccaaat cgaaggcttg
     1021 gtagttggga aaaatatctc tatggctgat cttcaaggaa cgcttcagtt gattgtccaa
     1081 aaaatgtttg gtgaagagcg tcaaattcgt ttgcgtccat cttacttccc attcacagag
     1141 ccatctgttg aggtggatgt ttcttgcttc aagtgtggtg gagaaggctg taatgtatgt
     1201 aagaaaacag gttggatcga aattatgggg gccggtatgg ttcacccacg tgtccttgaa
     1261 atgagtggta tcgatgcgac tgtatactct ggctttgcct ttggtcttgg acaagagcgt
     1321 gtagctatgc tccgttatgg aatcaacgat atccgtggat tctaccaagg agatgtccgc
     1381 ttctcagaac agtttaaata atgattagaa aagtagaaat ggcagatgtt gaggtgttgg
     1441 ctaaaattgc caaacaaacc tttcgtgaaa cctttgcgta tgataatacg gaagagcagt
     1501 tacaggaata ctttgaagag gcttatagtc tgaaaactgt gtcaactgag ttgggaaatc
     1561 ctgactctga aacctatttc attatgcatg aggaggagat agctggtttt ctcaaagtca
     1621 actggggaag tgctcaaact gagagagaat tagaggacgc ttttgaaatt caacgcctct
     1681 atgtgctaca aaaattccaa ggatttggac taggtaagca actgtttgaa ttcgcacttg
     1741 aacttgctac aaaaaatagt ttttcttggg cttggttagg tgtttgggag cataatacaa
     1801 aagctcaagc gttttataat cgatatggtt ttgaaaaatt tagccaacat cattttatgg
     1861 ttggtcaaaa agtagatacg gattggttac tgagaaagaa attaaggtaa gaagaggatt
     1921 cttctattga aatgatagga aaggaaaaga actagagtga caactgtcat tctggagaac
     1981 taaattatgc ttgtatctta taaatggtta aaagaattgg tggacattga tgtgccatca
     2041 caggagttgg ctgaaaaaat gtcaaccaca gggatcgagg tagagagtgt cgaatcacca
     2101 gctgctgatc tctcaaaaat tgttgtcggt gaggtcttgt cttgcgaaga tgtgccagag
     2161 actcacctcc atgtttgtca ggttaacgtt ggcgaagaag aagcccgtca gatcgtttgt
     2221 ggtgccccaa atgtgcgtgc tgggatcaag gtcatggtgg ctcttccagg agctcgtatc
     2281 gctgataact acaaaatcaa aaaaggaaaa atccgtggtt tggagtcact tggaatgatc
     2341 tgttcacttg gtgaattggg gatttctgac tcagttgtgc ctaaggaatt cgcagatggc
     2401 atccaaatct tgcctgagga tgccgtgcca ggtgaggaag tcttttctta cctagacttg
     2461 gatgatgaaa tcatcgaact ttccatcaca ccaaaccgtg cagatgccct ttctatgtgt
     2521 ggagtggctc acgaagtggc agccatctat gacaaggcag tcaactttaa agaatttact
     2581 ctaacagaaa ctaatgaagc tgcggcagat gccctttctg tcatcattga gacagacaag
     2641 gcgccttact atgcagctcg tatcttggac aatgtgacca tcgcaccaag tccacaatgg
     2701 ttgcaaaacc ttctcatgaa cgaaggaatc cgtcccatca ataacgtagt ggacgtgacc
     2761 aactacatcc tgctctattt tggtcaacca atgcatgcct ttgacttgga taactttgaa
     2821 gggactgaca tccgtgtgcg tgaagcgcgt gctggtgaaa aattggtgac cttggacggt
     2881 gaagaacgtg acttggacgt gaatgaccta gtcatcactg tcgcagacaa gccagtagcc
     2941 cttgcaggtg tcatgggtgg tcaagcaaca gaaatctctg aaaaatctag tcgtgttgtc
     3001 cttgaagctg ctgttttcaa tggcaaatct atccgtaaga caagtggtcg cctgaacctt
     3061 cgttctgagt catcttctcg ctttgaaaaa ggaattaatg tggcaacagt taatgaagcc
     3121 cttgatgcgg cagctagcct gattgcggaa cttgcaggtg cgacggtgcg taagggcatc
     3181 gtttcagcgg gtgagcttga tacttcagat gtagaagttt cttcaaccct tgctgatgtt
     3241 aaccgtgtcc tcggaactga gctgtcttat gctgatgtag aagacgtctt ccgtcgtctt
     3301 ggctttggtc tttctggaaa tgcagacagc tttacagtca gagtcccacg tcgtcgttgg
     3361 gatatcacaa tcgaagctga tctctttgaa gaaatcgctc gtatctatgg atatgatcgc
     3421 ttgccaacca gccttccaaa agacgatggt acagctggtg aattgactgt gacacaaaaa
     3481 ctccgccgtc aagttcgtac cattgctgaa ggagcaggtt tgacagaaat tatcacctat
     3541 gctctgacaa ctcctgaaaa agcagttgag ttcacagctc aaccaagtaa ccttactgaa
     3601 ctcatgtggc caatgactgt ggatcgttca gtcctccgtc aaaatatgat ttcagggatc
     3661 cttgataccg ttgcctacaa cgtggctcgt aagaataaaa acttggccct ttatgagatt
     3721 ggaaaagtct ttgaacaaac aggtaatcca aaagaagaac ttccaaatga gatcaacagc
     3781 tttgcctttg ccttgacagg cttggttgct gaaaaagatt tccaaacagc agcagttcca
     3841 gttgatttct tctatgctaa gggaatcctt gaagccctat ttactcgttt gggactccaa
     3901 gtaacctata cagcaacatc tgaaatcgct agccttcatc caggtcgtac agccgtgatt
     3961 tcactcggtg accaagttct tggtttcctt ggccaagtgc atccagtcac tgccaaggct
     4021 tacgatattc cagaaacgta tgtagctgag cttaaccttt cagccatcga aggggcgctc
     4081 caacctgctg ttccatttgt ggaaatcacc aaattcccag cagtcagccg tgacgttgcc
     4141 cttctcctca aggcagaagt gactcaccaa gaagttgtag atgctatcca agctgccggc
     4201 gtgaaacgtt tgacagatat caaactcttt gacgtcttct caggtgaaaa actgggactt
     4261 ggtatgaagt caatggctta tagcttgacc ttccaaaatc cagaagacag cttaacggac
     4321 gaagaagtcg cacgctacat ggaaaaaatc caagcgtctc tcgaagaaaa agtcaatgca
     4381 gaagtgcgtt aaacgatagt aggatacaaa ttccccctct agtttatgaa cactagaggg
     4441 gtgattttat ataaaataat taaaatgtga tttctgtaaa aatacatcta tgatcagagt
     4501 tttgaaaact tacaatgtct aaatctttaa catagtagtg gtttttaggc aataaaatat
     4561 gatctattgt tgcattaaaa agttttggac tttggctatt ccaagttcct ctttcaaaag
     4621 gtggaagtgc attcaatgcg tccctatgag agcttatttt tgcaagtgct ccatgacgca
     4681 tagttgcatt aaaatcacct gcaataatag cctttggata ttttgaagcc aattgattat
     4741 gaatgatgtt taagtcttgc ttccagattt ccattaaacc tggcagagga ggagcagtat
     4801 gcaaggcaat gatatctggt atattttgtt ttctcgaatg taatacaatt gtcccgaacc
     4861 gtgttgtatg aaaagtttta gcttctgtat agaaaccata acttttcttg acaatcacag
     4921 tcacaggagc tattccacta ttggtaggtg gagaagtgaa aatatcatag ttggccatag
     4981 aaagtccaac ttgatgaaac aatagtttga ttctctggtt ttcttgctca cctctgatat
     5041 tggtagctag ttcagggaat atagccatat cggcgtcaaa atggctaaaa attcgctcaa
     5101 tatgttgtgc ttctatttga ttagcgacgt tccaagttac taattttaag gttttagtct
     5161 tatttgatgc agaagaaagg gttttatctg ttccgaataa tagtaatatg agcgatagta
     5221 agagcaaaat gccagagtaa aaccgtattt gtttattttt cttgtaaaaa gataggaaaa
     5281 aaaataagaa acttaaaata gcagccatag ttgcagtagc taaggggaag gcataaatct
     5341 gtgccaagat aggttgggta gatagccaaa tgttaagatt agaagatggg aaaaaagaat
     5401 aagttatgat agggatagaa aagagaatga ccgaaagggc agagagaaat gttttaatca
     5461 ttgtgtgagt acctcaattt gcaatggttg ctcggagcta atgtgacttt gtgactttac
     5521 ttgattgata ttctttttat tgtcttcctt atagattaag tacaacccaa gtaaaaaaag
     5581 tccaatttaa gattgaaatg attaaaaggc acagagatgc aagacaatta tactacaaaa
     5641 ggtaaacatt tgacaatcga tagccgtcgc ttaatcgaaa gatggaaaaa agaaggaaaa
     5701 tcaaatagag aagttgcctc tctacttgga aaagctcctc aaactatcca cactgaaatc
     5761 aagcgtggga cagtccgaaa atgtcttgga aaagggcgct tcaaagaggt ttattctgcc
     5821 gactacgctc aacagtctta tgaaaacaat cgcaagcgct cggtcaagaa atcaagcttg
     5881 accaaggaac taaaggaaaa gattctccac tatacataac caaaaatttt cgcctgaaat
     5941 gatggttatg gctaaagggg ttaacgtggg aatttcaacc atttactatt ggattcatcg
     6001 tggaaaattg gggttaagca aacaggattt gctttatcct agaaaaggaa aatctgttaa
     6061 gaaacaagtt agtcccaatt ttaaacccgc tgatcaatct atcgaatcat aattcgctca
     6121 ttttttcaaa aaatacatag atctaaaaga aagaggggta agtgatgaag aatagtgcta
     6181 ttgggagtaa ttggaaggat gtctatctga gctctttagc aaggaggaaa tccttgaaag
     6241 tgatatgcga gtagctatca tgagcgagtt gattgaagcc agaaataagc aaggaatcag
     6301 tcagaaaaag ctagaggaag tcagtggagt gagtcagcct gttatagcta ggatggagac
     6361 aggtaagact agtccacagt tggacacagt cttaaaagtc ctagctagtc taggaaagac
     6421 actagcagtc gttccacttg aacaggggaa aagttgacta gaatcagtta tagtcttttc
     6481 taataacaag ccatagtcac ttataagaat tactaataac gcgtttgagc attctaacta
     6541 aaatacagct aaaaacaagt tataaaaggg atttgagacg tttgcttcaa gtctttttct
     6601 tttgtccgaa acagatatat agtttcaaac tatatcaaac cctaattttt tacaattata
     6661 cagacgcttt cttttctgtt aagatagttt caacaacaaa ttttggagga cacatcatgt
     6721 caactacaat cgtcggtttc cctcgtttgg gcgaattccg tgaattaaaa tttacaactg
     6781 aaaaatactt tagaaaagaa atctcagaag aagaactttt agcagcagca aaagacttgc
     6841 gtgctaaaca ctggaacatt gtcaaagaaa aaggcatcac tgaaattcca tcaaatgact
     6901 tttctcacta tgataacttc ctagatgcgg ctttcctttt caacgtggtg cctgcatccg
     6961 ttcaaaactt ggacttgtct gaccttgagc gctacttcgc tttgggacgt ggttaccagg
     7021 gagaaaaagg ggacgttcgc gcccttccaa tgaagaaatg gttcaacact aactaccact
     7081 acatcgttcc taaatttgaa aaagacactc aagtaaaatt ggctggtcac aagattttcg
     7141 atgagttcca agaagccaaa gaacttggat tgaacactcg tcctgttctt gtaggtccct
     7201 tcactttcct tcaattgtca gactttgaag aaggcgtgaa agcagacgac ttcgtagata
     7261 gcttagtgtc tgcttaccaa gaagtttttg ctaaattggc tgaacttggt gcgactcgca
     7321 tccaattgga tgaagcggct cttgtaaaag acttgacagc tgaagaaaaa gctcttttct
     7381 tgaaccttta caacaaactc ttggctgaca aaaaaggtct tgaagtcttg cttcaaactt
     7441 atttcggtga tgttcgtgac gtatacgctg accttgtaaa cttaccagta gatgctatcg
     7501 gtcttgactt tgttgaaggt aagaaaactc ttgaactcgt taaaggtggc ttcccagctg
     7561 acaagactct ctatgtaggt attgtcaatg gtaaaaacat ctggcgcaac aactacgaaa
     7621 agagcttgac tgttcttgag caaatcccag ctgaaaacat cgttttgaca agctcttgct
     7681 cacttcttca tgtgccattt acaactgcta atgaagaatt tgaaccagca ctcttgaacc
     7741 actttgcctt tgcagttgaa aaattggatg aaatccgtga cttggatgct atccgaaatg
     7801 gtcaaggttc agaagctctt gcagccaaca aagaactctt tgcgactgag cgtgttggtg
     7861 aaaatgctga acttcgtgcg cgtatcgctg gcttgacaga cgcagactac actcgtttgc
     7921 cagcctttgc agaacgtgaa gctatccaag aagaagcttt caaacttcca gctcttccaa
     7981 caacaacgat tggttcattc cctcaaacaa aagaagttcg tgccaaacgt ttggcttacc
     8041 gtaaaggtga attgtctcaa aaagagtacg acgctttcct tgctgaaacg attgacgaat
     8101 ggatcaagtg gcaagaagat attgactttg atgtccttgt tcacggtgaa tttgagcgta
     8161 atgacatggt tgagtacttc ggtcaaaact tgtcaggata cctcttctct aaaaatggat
     8221 gggtacaatc atacggtatg cgtggggtaa aaccaccaat catctggggt gatgtcactc
     8281 gtcttaaccc tatcactgtt aaatggtcta gctatgcaca aagtcgtaca aacaaacctg
     8341 ttaaaggtat gttgactgga cctgttacca tccttaactg gtcattccca cgtgaagaca
     8401 tctctatcaa ggattcaact ctccaaatcg cccttgctat caaggatgaa gtacttgacc
     8461 ttgaagctgc tggtgtgaaa atcatccaaa tcgacgaggc tgctcttcgt gaaaaattgc
     8521 cactccgccg tagcgactgg tacgaagact accttgactg ggctattcct gccttccgct
     8581 tggtacactc aacagtagcg ccagacacac aaatccacac tcacatgtgt tactcagaat
     8641 ttacagatat catcccagct atcgataaca tggatgcaga cgttatttcc tttgaagcta
     8701 gccgttcaaa ccttgaaatc ttggacgaac tcaaagcgaa aaacttccaa acagaagtgg
     8761 gacctggggt ttacgatatc cactcacctc gtgtaccaaa tgaaggcgaa atcgacaaca
     8821 caatcgaagt catccttgct aaagtgccaa gcaagaaagt ttggatcaac cctgactgtg
     8881 gtttgaaaac acgtggtatc ccagaaacaa aagaaagctt gatccgcctt gttgaagcag
     8941 ctaaagctgc gcgtgagaaa ttgtaagatt ggatttctct ccatacagtg gttgttcaaa
     9001 aagaaatcaa ctagaaaagg ttattacata tgtcacgcca aacaccgtca ctctcatttg
     9061 aagtgttccc tccaaaccca gccgtgggta atgataacat tatttcagcc ttgcaggata
     9121 tgcaggagtt ggctccccat tttatcagtg taactgccag caataataaa tttaatatca
     9181 aggaaacgac ggttcgtttg gctgacttta tccaaaatga tttggcgatt ccgaccattg
     9241 cccacttgcc agctatttat ctaactaagg acaaggttac tgaaactatt gctgacttgg
     9301 acaaagttgg ggtgcagaaa atcttggctc ttcgtgggga tattattcca gacgtggaac
     9361 cacaaaagga tttccgctat gcaaccgact tgattgagct tatcaaggaa cgagcccctc
     9421 actttgatat tattggagct tgctatccag aagggcatcc agattcgcca aatcagattt
     9481 cagatattca aaatcttaag aagaaagtgg atgcaggctg ttcgagcctc gtaactcagc
     9541 tcttctttga caatgagcgc ttctatgatt ttcaggacaa gtgcatcttg gctgggattg
     9601 atgttcccat tcatgcagga attatgccaa ttctgaatcg aaatcaggct ctccgactct
     9661 tgaagacttg tgagaatatc catcttccac gcaaatttaa agccatctta gacaagtatg
     9721 agcatgaccc tgagtcgctc agagcagcag gacttgccta tgcagtggac caaatcgtgg
     9781 acttggtaac tcaggatatt gccggtgtgc atctctatac gatgaataat gctgatacag
     9841 caaaatacat ccatcaagca acccatgcct tgtttaatca ccagtctcta ggataataaa
     9901 agcaaaccat tcttctcagg tgaggggaat ggttcctttt taatggtaaa gacctcactt
     9961 tttaagaaaa atatgataaa ataaattctg tacgtacttg atacaaagat gaaggtatta
    10021 aataatacaa tgcatttaaa ctgtaagatt taacgacacg agtgagtcaa atcgatgtta
    10081 tttgacgaga gagttagtaa agcatttagc taatcgcccg atagcgatta gcgtgaaagc
    10141 tttagatact ttagtattaa acctttctaa tgatgtatct tgaaaactga acttgcctac
    10201 aactctgtgg aaaaagataa atctccctag gagcagtcgc tccgtcgttc gatttcctat
    10261 tttcctttga gttgcttgac ggcttagtat cttgagtaat tgaattttgg tctcatttct
    10321 taggaaaaaa gataagcttc ctagcactcg tcgagtgcga cgtcaccttc ctatttttct
    10381 acagaaatgt tcggcaagcc gaaccgtcca aaatatcttg tgtaattgaa cacggccgaa
    10441 aagctgtgta aaaaagataa actgtcttgt cttcatcgaa gacttcgtca gtttcctatt
    10501 tttactttgc ttttgacgtc cttggtatct tgatctttgt aggcaaggcg tataatttca
    10561 tcaatccaaa ggggattaaa atggcaaaac aagtgtttca aacgactttt gcgggtcgtg
    10621 agttaattgt agagactggt caggttgcta agcaagcaaa tggatctgtt gtcgtacgtt
    10681 acggtgagtc aactgtcttg actgctgccg ttatgtctaa gaaaatggca actggggatt
    10741 tcttcccact ccaagtcaac tacgaagaaa aaatgtatgc ggctgggaag tttcctggtg
    10801 gctttatgaa acgtgaagga cgtccttcaa cagatgcgac cttgacagcg cgtttgattg
    10861 accgtccgat tcgtcctatg tttgcggaag gtttccgtaa tgaagtccaa gtcatcaata
    10921 cagtgctttc ttatgatgaa aatgcatctg caccaatggc tgctatgttt ggttcatctt
    10981 tggcactgtc tatttcagat attccatttg acggaccaat tgctggggta caagtgggat
    11041 atgtagatgg ccaaatcatc atcaacccaa gtcaagaaca agcagagcaa tctcttcttg
    11101 aattgacagt agctggaacc aagcacgcta tcaacatggt agagtctggt gccaaagaat
    11161 tgtcagaaga aatcatgttg gaagcgcttc ttaaagggca cgaagctgtc aaagaattga
    11221 ttgccttcca agaagaaatc gttgctgctg tcggtaaaga aaaagcagaa gtggaattgc
    11281 ttcacgtgga tgctgaattg caagctgaaa tcattgcagc ctacaacagt gacctccaaa
    11341 aggcagttca agtagaagag aaattggccc gtgaagctgc gactcaagta gtgaaagacc
    11401 aagtgactgc cgtttacgaa gaaaaatatg cggaccacga agaatttgac cgtattatgc
    11461 gtgatgtggc tgaaatcttg gaacaaatgg aacacgcaga agtgcgacgt ttaattacag
    11521 aagacaaggt gcgtcctgat ggtcgtaagg tcgatgaaat ccgtcctttg gatgcggttg
    11581 ttgacttcct tcctcgtgta catggttcag gtctctttac tcgtgggcaa actcaagctc
    11641 tttcagtctt gaccttggct ccgatgggag aaactcaaat cattgatggt ttggatccag
    11701 agtacaagaa acgctttatg caccactata acttccctca atattctgta ggggaaacag
    11761 gtcgttacgg tgcgccaggt cgtcgtgaaa tcggtcacgg tgcccttggt gagcgtgctc
    11821 ttgctcaagt cttgccaagc ttggaagaat tcccatacgc tatccgtcta gtagcagaag
    11881 ttttggaatc aaacggttct tcatctcaag cttctatctg tgcgggaact cttgccctta
    11941 tggctggtgg tgtgccaatc aaggcgccag tagctggtat tgctatggga cttatctcag
    12001 atggaaataa ctacacagta ttgacagata tccaaggttt ggaagatcac tttggagata
    12061 tggacttcaa ggttgcaggt actcgtgatg ggattacagc ccttcaaatg gatatcaaga
    12121 ttcaagggat tactgcagaa atcttgacgg aggctcttgc tcaagccaag aaagcgcgtt
    12181 ttgaaatcct tgatgtcatt gaagcaacca ttccagaagt tcgtccagaa ttggctccaa
    12241 ctgctccgaa aattgatacg atcaagattg atgtggacaa gattaagatt gtcatcggta
    12301 agggtggaga aaccatcgac aagattatcg ctgaaacagg tgttaagatt gatatagacg
    12361 aagaaggaaa tgtgtctatc tactctagtg accaagatgc tattaaccgt gccaaagaaa
    12421 ttattgctgg tttggttcgt gaagccaaag tggatgaagt ttaccgtgct aaagtcgttc
    12481 gtatcgagaa atttggtgcc tttgttaacc tctttgataa gacagatgcc cttgttcata
    12541 tctctgagat ggcttggact cgtaccaatc gtgtagagga tttggtagaa atcggggatg
    12601 aagttgatgt taaggttatc aaaattgatg aaaaaggccg tatcgatgcc tctatgaagg
    12661 ctcttctacc tcgtccgcca aaacctgagc atgatgaaaa aggtgaaaag tctgagcgcc
    12721 ctcaccgccc acgtcatcaa aaggattaca aacctaagaa agaatttaca gaaacatcaa
    12781 aagattcaga ataagaaaag gagaaatgta tggggtggtg gcgcgaaacc attgatattg
    12841 taaaagaaaa tgatccagcg gcccgcacca ctttggaggt tttgctgact tatccaggtg
    12901 tcaaggcctt ggcggcccac cgtctctcgc attttctctg gaagcacggc ttcaaactat
    12961 tagctcgtat gtacagtcag ttttggcgct tttggactca gattgagatt catccaggag
    13021 cccagattga ttcaggtgtt tttattgacc atggttctgg tctggtgatt ggagagacag
    13081 cgattgttga aaaaggcgtt cttctctatc acggagtgac tctcggggga acagggaaag
    13141 actgtggcaa acgccatccg actgtacgaa agggagccct catatcagcc catgcccaag
    13201 ttatcgggcc tgtggaaatt ggtgaaaatg ccaaggtcgg tgctgcagca gttgtcgtag
    13261 cagatgtacc tagtgacgtg acggttgtcg gtattccggc caagattgtc cgtcttcatg
    13321 gtaagaaaga tgagcctgtc attcacgaag tcgaagaaaa acgagagtat tatgtcaata
    13381 aactcgagca ggctaaagat gccagtcaca gatcgtctgg tttgtagagg atacctatat
    13441 gattcaagca agaaacaagt taagccaaga ggagctatct gaggcgaaaa aagtaattaa
    13501 ctgttgccaa aactatgacg gtacctatcg cgatccctat ctctctaaca tgcttaattt
    13561 tgacccaaac atgcccgcct ttttccttta ttatgaaaaa ggcgaacttg ttggtttatt
    13621 aactgtctat gcagatgacc aagatgtaga agtgacgata ctggttcatc caggtcatcg
    13681 ccgtcagggg attgcgcgtg cattgtttac tagttttgag agagaaacag cttctttccc
    13741 cattcggtca gtcacttttc agacagaacg tattttttta gagaatcatc ctgactttgc
    13801 cagtaactgg ggactaatcg aggatgaaga gacagaaact tggttaggta aggatagaag
    13861 accttatccg ttagcaaatg tttcccatct tgaagttttg ttagcagata gttcgtatca
    13921 ggatcaaatt agtcaattaa aatttcaggc attttcagag gaacatgaat cgagagaagt
    13981 tgtggataga tatgtcgctg aagctctgaa agatccagaa agccgactat atattttatt
    14041 aaaagctggt caggttattg gaacttgtac ggttgattta tcgactaata cgaattacct
    14101 ctacggttta gcaatattgg aacctgaacg tggaaaaggc tatggaagct acttagcaaa
    14161 atccctcgtc aaccaactaa ttgagcaaaa tgacaaggaa tttcagattg cagtggaaga
    14221 tagcaatgta ggtgccaaac gtttgtatga aaaaattggc tttgtcaaac agactcaggt
    14281 ggtttatctg aatgagaaag gagcaaggga ttccgaagtg tagagatatt cggactgaaa
    14341 tttacttgaa cttttagtga tgaaactaat tgttcttgga tttcagcttt cctgattatg
    14401 atttatgatt aaaatctatg acaccatgtc tcgtgatttg cgagaatttg tcccgattga
    14461 ggacggcaag atcaagatgt atgtttgtgg gccaacggtg tacaactata tccacgtagg
    14521 aaacgcccgt tcgacggtag cttttgatac cattcgtcgc tattttgagt accgtgggta
    14581 taaggttgcc tatatttcca attttacaga tgtggatgat aagattatca accgtgccag
    14641 ggaagaaggt atcacgcctc aggaggttgc ggataagtac atcgctgcct ttcgtgagga
    14701 tgtgacggca ttgggcgtga aacctgcgac tcgccatccg cgtgtagtgg agtttatggc
    14761 agacatcatc cgttttgtgg aagacttgat cgagaaaggc tttgcctatg agagtcaagg
    14821 ggatgtctat ttccgtgtag aaaaatccca caactatgct aaattggcta ataaaacctt
    14881 ggaagatttg gagctaggtg cttcaggtcg taccgatgaa gaaacggctc gtaaggaaaa
    14941 tcctgtagac tttgctctat ggaaatcttc caaaccaggt gagatttctt gggatagtcc
    15001 ttggggacct ggacgtccgg gttggcatat tgagtgttca gtcatgtcga cagagatttt
    15061 aggtgatacc attgatatcc acggtggtgg agctgaccta gagtttccac accacaccaa
    15121 tgaaattgcc cagtctgaag ctaaaacagg caaggctttt gctaactact ggatgcacaa
    15181 tggctttgtc aatatcgaca atgtcaaaat gtctaagtcc ttgggtaact tcattacggt
    15241 acacgatgcc cttaaaactc ttgatgggca agtacttcgt ttcttctttg cgacccagca
    15301 ctaccgtaag cctatcaact tcacggaaaa ggcagttcgc gatgccgaga ccaatctcaa
    15361 gtatctcaag aatacttacg aacaaccatt tacaggaaat gtggatgccc aagagttaca
    15421 aaactttaaa gataagtttg tagcagctat ggatgaagat tttaatgctg ccaacggtat
    15481 cacagttgtc tttgaaatgg ccaaatggat caactcaggg aactatgatg caagtgtcaa
    15541 gcaagctctt gcagatatgt tagaaatttt tggaattgtc tttgttgagg aagttttgga
    15601 tgcagagatt gaagacttga ttcaaaaacg ccaagaggcg cgtgccaatc gtgactttgc
    15661 gacagcagac caaatccgtg accaattggt tactcaagga attaagctcc ttgataccaa
    15721 ggatggagtg aggtggacac gtgattgatg tcaatctcat taacgggatt gcgctagcct
    15781 ttgaggggga tgcggtgtat tctatgtata ttcgccgtca cctcatcctc aaaggtatga
    15841 ccaaacccaa taaactccat caagaagcaa ccaagtatgt gtcagccaag gctcaggctc
    15901 gcctgattgc tctcatgttg gaggagcagg tcctaacgga aaaagaagaa gaaatctaca
    15961 aacgtggccg caataccaat agccacacaa aggctaaaaa tgcagatgtc gtgacttatc
    16021 gtatgtccac gggatttgaa gcggttatgg gctatctcca tatgactgag aatctggaac
    16081 gtcttgagag tttggtttca tggtgcatcc aaaaagtgga gggctagaac atgagggaaa
    16141 aagaactaca agactggttt cctgatgctc ggatttcaga ccaaccagta gagaaagagg
    16201 gctatctcac gctcccttta gcttctcagc agtggatttt gctggaggaa gctgggctca
    16261 gcgagcgtga aaagcagttg gttgcccttt tgacccagca ggagcaggct cgttcgctaa
    16321 acccttggta ttcctatctg gttgagggca agggacaggc accgcaagtt tttaaaaaga
    16381 ttcagttggt ttattgccat ctttcttatt ttcagcagga aaatctggct tcttggctag
    16441 atatgatgcg gactcttttt ccgaattgtc agacagtgct acaggtcgga gctcaggatt
    16501 atgttttcgt gcttcaacaa gacaaataca cttctgtaac agatatttta agtgatatga
    16561 ttgaagcggt tgagtatgac tttggacttc gtctttctat catgttgggt caggtttggt
    16621 ctcagatagg acatcaagcc ctatcagact taatcaaagc tgagcgggat ttgttcaaga
    16681 catggtggcg tcagggtcac caaggtgttc atactttttc tcagctctat ctttggagta
    16741 tgggagaaag actcgtggac ttgaagccaa ttaaagaatg tctacaccag atgattttgg
    16801 atcaagatca gattcaggaa atcattctct ctctttggga aaatagtgct gttctcacta
    16861 aaacagccca gcaactctat ctgcaccgca attctctcca atacaagatt gataaatggg
    16921 aagagttgac agggcttcag ttgaaagagt tgaccgacct gaccttgtgt tatcaattga
    16981 ttttaggttc tttgtcaact atagttggtt tgtaaagaag ttaatatttg gagaagagga
    17041 ttgccatctt ctccattttt atgtgcagag ttatagtggc ttgatgctgg gaaagtacac
    17101 tgtgactgct aaaacattcc tagaagctgc tttgatttcc ctaatctatt tatgcaaatt
    17161 ttatgttatt ttactataac agttgttgct aagccaaata atagtgggga agttcattta
    17221 gacgtaagca ttgaagataa tcagggaggt agtgggcata atttcagttc tgtttcaagt
    17281 agctcacaaa cagctaaata tgaagaaact ggttataata acaattcatc attatatata
    17341 acgattgata aaacgtctga tgcaacagct cttttgaaat taaagttgaa taatgttgat
    17401 aatcaacctg ctactgaagt tcctagttca ggaattactg taaaattaaa tgctaaagat
    17461 aatgctggaa actggacaag tgcttcgaat aaaaaagaaa taacagtaaa aattgtttct
    17521 gctaaaccga catatccaga caaaatctta gtgaaaaatc ctgataatat aaaagataca
    17581 gaaaaaaatg ccattattga aaaattgaaa gaggcaaata aaaatcatcc agcaggagct
    17641 ccaacctttg ctaaaggtga aggagagcat gcaaatgata ttgtagcaac ttattcagat
    17701 ggtacaactt attatgtacc gttaaatgat gtgacaaaat atgcgaggta gtggctgtac
    17761 cactcactta ttcacctccc cgtgatttgt agtagtgata ggctttctca ctattattat
    17821 aaaacaaaat aaagatcaca acactttttc attctgtgtt gtgccttgag tgaaacgaaa
    17881 ggaatgaatt ataaatatga aaagtatagt cactagcata gatgagcgct tgcgtactcg
    17941 cctacgagtg attatctgga agcaatgg
//
LOCUS       DAVKGC010000032        17841 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKGC010000032 DAVKGC010000000
VERSION     DAVKGC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17841
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.032"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(96..168)
                     /locus_tag="VW049_000899"
     tRNA            complement(96..168)
                     /locus_tag="VW049_000899"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Lys,seq:ctt)
     gene            complement(433..1704)
                     /locus_tag="VW049_000900"
     CDS             complement(433..1704)
                     /locus_tag="VW049_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9432053.1"
                     /translation="MPDNLALRMRPKTIDQVIGQDHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYTTMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            1974..2213
                     /locus_tag="VW049_000901"
     CDS             1974..2213
                     /locus_tag="VW049_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432054.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVYSNNGLKKYEK"
     gene            2378..2557
                     /locus_tag="VW049_000902"
     CDS             2378..2557
                     /locus_tag="VW049_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432055.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            2694..2873
                     /locus_tag="VW049_000903"
     CDS             2694..2873
                     /locus_tag="VW049_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9432056.1"
                     /translation="MPMTQKEMVKLFTAHGWIKTRGGKGSHIKIEKQGERPITILHGK
                     LNKYTERGIGKQAGL"
     gene            2910..3362
                     /locus_tag="VW049_000904"
     CDS             2910..3362
                     /locus_tag="VW049_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9432057.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFGYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            3653..4123
                     /locus_tag="VW049_000905"
     CDS             3653..4123
                     /locus_tag="VW049_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9432058.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            4142..5245
                     /locus_tag="VW049_000906"
     CDS             4142..5245
                     /locus_tag="VW049_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9432059.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQKIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERATTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNFWFPRL"
     gene            5211..5369
                     /locus_tag="VW049_000907"
     CDS             5211..5369
                     /locus_tag="VW049_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEU9432060.1"
                     /translation="MELEISGFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
     gene            5479..6513
                     /locus_tag="VW049_000908"
     CDS             5479..6513
                     /locus_tag="VW049_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9432061.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            6515..8758
                     /locus_tag="VW049_000909"
     CDS             6515..8758
                     /locus_tag="VW049_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEU9432062.1"
                     /translation="MNTLLTLRGKSFTPKSAKGRGPITIPADSIVTLNHLNELYTSLE
                     RVKEYWSNNTIIDGVLISVYYNRVLAKSNRIDGFFNVGGGNAIPNETIVGAKFSNDKS
                     RHIITHYISKEVLDKTIEHTKKVITIFKDYFASAPMTSMVFSDSSKFNQIDYSKYGLS
                     KSKFKQYLRDACFVKGFGIEQADMSTIRNSIVTIYDVKTNLLTLLKQLDIDVSEANIM
                     DQTTVLLDEKNIELLLLKAPYLVSMIVEDFSSLSPENFYNDGNTFYNNLPFPTNEPII
                     GVIDTLFDDGVYFNEWVEYHDLISPDISKGSQDYKHGTAVSSVIVDGASLNPKLDDGC
                     GRFRVRHFGISLQSGFNSFTIIKQIREIVSQNPDIKVWNLSLGSNDEIRENFISVEGS
                     LLDEIQFDNDVIFIIAGTNGIAPDGQRKKIGAPADSLNSIIVNSVDFSNQVVSYSREG
                     VVLSFFIKPDVSYYGGGNGDFINVCEPLGLARVAGTSFAAPFIARKMAYLIHIMGLSR
                     EEAKALLIDSAIPWNDKKTFADISLLGYGVVPIKIEDILSTPDDEIKFIVSDVSRAYE
                     TYNYDFPVPISKEHYPYVAKATMCYFPKCSRNQGVDYTNTEMQLSLGVLDKKGIKTIN
                     NDNQYSENAPGYVREKSARNIFRKWDNVKHLGEKFSSRKKAKPILNPSNPQWGMSVKT
                     IERLRNDDGYGIRFGVVVTLKELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSL
                     NEEIEFE"
     gene            8809..9828
                     /locus_tag="VW049_000910"
     CDS             8809..9828
                     /locus_tag="VW049_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9432063.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYKDFYEFQNRTYISRKENEEEYSN
                     LIYESLFSDTVNDYENWSDFELSIGKLTKDNDLISSSIEIKEKFIDDFSEVVDDLREY
                     LRIQQEKNLEKGNAIDFISTLDNMRTSLPVINQPAIDKKYNENLYQHDIVNILTLNYT
                     NVIDKLYNESAKSFSNQLRTNNYKFHIAPPIHAHGTVDICTVLGVSDETQISNSFDEE
                     QKESLIKNLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDRYIWSQIAERSISGS
                     VPVIIYHYVPHFDPGNPIRAKRLYRNVEDKFIQNSGIDLELEKKLRDNLIVVIGKTIF
                     NLMER"
     gene            9841..10791
                     /locus_tag="VW049_000911"
     CDS             9841..10791
                     /locus_tag="VW049_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9432064.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTVLA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRELAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            10793..11536
                     /locus_tag="VW049_000912"
     CDS             10793..11536
                     /locus_tag="VW049_000912"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9432065.1"
                     /translation="MQQYFVKGSAISPVTIENKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNVVPSIHLFENKADFLAQLDQ
                     FDRILVAYEEAAKEGEAAALIKAVTGLEKGAKLLFIFGPEGGLSPAEIENFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLLELEK"
     gene            11547..13343
                     /gene="pepF"
                     /locus_tag="VW049_000913"
     CDS             11547..13343
                     /gene="pepF"
                     /locus_tag="VW049_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9432066.1"
                     /translation="MEQKHRSEFPEKELWDLTVLYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQTGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            13589..14143
                     /locus_tag="VW049_000914"
                     /pseudo
     CDS             13589..14143
                     /locus_tag="VW049_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
     gene            14254..14922
                     /locus_tag="VW049_000915"
     CDS             14254..14922
                     /locus_tag="VW049_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9432067.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(15043..15120)
                     /locus_tag="VW049_000916"
                     /pseudo
     CDS             complement(15043..15120)
                     /locus_tag="VW049_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(15132..15665)
                     /gene="queF"
                     /locus_tag="VW049_000917"
     CDS             complement(15132..15665)
                     /gene="queF"
                     /locus_tag="VW049_000917"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9432068.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(15970..16284)
                     /gene="trxA"
                     /locus_tag="VW049_000918"
     CDS             complement(15970..16284)
                     /gene="trxA"
                     /locus_tag="VW049_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9432069.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            complement(16301..16522)
                     /locus_tag="VW049_000919"
     CDS             complement(16301..16522)
                     /locus_tag="VW049_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9432070.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            complement(16621..17001)
                     /locus_tag="VW049_000920"
     CDS             complement(16621..17001)
                     /locus_tag="VW049_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9432071.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            17311..17412
                     /locus_tag="VW049_000921"
     CDS             17311..17412
                     /locus_tag="VW049_000921"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432072.1"
                     /translation="MKEGHRHVEAAKVFDVDARTLFTWEKKDMNKGT"
     gene            17379..17840
                     /locus_tag="VW049_000922"
     CDS             17379..17840
                     /locus_tag="VW049_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9432073.1"
                     /translation="MGKERHEQRNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTASFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
BASE COUNT         5440 a         3080 c         3950 g         5371 t
ORIGIN      
        1 aagtttgttg taaaaacacg gcaaattgca cgacaatttt taaaaggaaa gcaaaaaaag
       61 ccctatatta taaggctttt cctgttgtat ttatatgccc cctacaggga tcgaacctgt
      121 gacccacgga ttaagagtcc gctgctctgc cagctgagct aaggaggcaa agaaaaaagc
      181 tgtattggtg ccgaaacttc acggtttgta ttgaacccgc gcaattaagc aggtgggcaa
      241 ctcgctctaa ctgaagctgt ttccgtgtga gacggcctac atgctgttag aagacttttg
      301 tttccctaat aatacaaaaa atagtcggtc aacacttaag tgtgaagtcg tacaccacag
      361 cgtttctata tttatatgat accacttttt caaaaaaaat caagaggaaa gtgcaatttt
      421 ttgaaaagga tttcagattt ttcgcaaacg gtcgatagct tcctttcttt gagccaaagc
      481 ccgttcatat ttaccagtat cttctgcttg gaaatagtga tgattacgaa ttttttctgg
      541 cagatagtct tgcttgaccc aatttccagg atagttgtgt ggatagagat agtcttgggc
      601 attccccagt tccttgcttc cactgtagtg cccatcacgc aggtgtcgcg gaataggcaa
      661 gtgccctgat gttttgaggt cagcaagtgc cttatccata gctacatagg ctgagttgga
      721 ttttggagaa agggccaaat caatcacgac attggcgatg agaatgcggg cttctgggaa
      781 accaatcctt tgtgcagcat ccagagcagt cacggtatga atctgggcct caggattggc
      841 taaaccgata tcttcatagg cgataacagt caagcgacga gcgagactag gcagatcccc
      901 agcctcaatc aagcgggcag catagtggag actggcatcc acatctgagc cacggataga
      961 cttttgcagg gcagatagaa catcatagtg accgtctcca tccttgtcca tagtggtgta
     1021 gcttctctga agactatttt ccatgatgtc taaggtgatg tggcgaatgc cctcgtcatt
     1081 ctcaggggta gagagaacag ccaagtccag tgagttaaag gcagagcgaa ggtctccgtt
     1141 tgtagaggtt gcgatgaaat ccagcgcatc ctcatctagt tctattggaa aatcaaaacc
     1201 acgttcaggg ttacttagag ctatctgcag ggcctctttg acgtcttggt tagacagagg
     1261 ttccaactca aaaatttgaa cacggctacg aatggcagga gtgacggaaa agaaaggatt
     1321 ttcagtcgtt gctccaatca tgatgaccag tccactttcc aagagaggca agaggaagtc
     1381 ttgcttggtc ttatctagtc gatgaatttc gtctagcaat aggacgagac caccagaaaa
     1441 tttagcttct tccgagattt cttgcagtct ctttttacta tcaactgtcg cattgaaagt
     1501 tcgaaaggca tacttggtcg ttccagcgat ggcagaggca atactggttt tgccgattcc
     1561 cggagggcca tatagaatca tggaggacag gcggttggct tccaccatgc ggcggatgat
     1621 ttttccaggt ccgaccagat gatcctgacc gatgacctgg tcgatggttt tagggcgcat
     1681 gcgaagcgcg agattgtctg gcatagcagt cctttctagc gtggatttga gtagaatgac
     1741 tcatctacta tatcttacta tgtccattct agcctattag ttgtgatatt tcaatggttt
     1801 tgtaataatc agggagtgag atagaatctt ttttactatt taatttaatg aggagaggta
     1861 tcagcatgag agatttgatt ttccttaaag ctcaaaatag gatagttgag cctaaagaag
     1921 tggaagtgct gacttgagaa ataatattgc tgaatgtcat tgtatgaact ataatgaaaa
     1981 gagaggtgat aagcaatgga aatgatggac caagtcagga aattttgatt tttacaaaac
     2041 aaattcgtca ttggattctt agtgatcaag ttatttcagg gaaaagaaaa cttttcttcc
     2101 gggaagatac tccaaaagag attttagatt tgtacgaaaa cattaaatct aaacttgatt
     2161 ttgcttacca agaagtttat tctaataatg gattgaaaaa atatgaaaaa tagaataatt
     2221 gatgtttttg aagtagtaaa tcgcctcctt gtaataactg ttgaaaatcc tgatttcgaa
     2281 gacttgagag ttaatcatac tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag
     2341 gctgtacttg agtacggtaa ggcgacgttg acgtggtttg aatttgattt tcgaagagta
     2401 tcagtttgta aaaattggag ataaaaaata tagagtacgc agtggtccta tgattcattc
     2461 aactccgcct caatctgtct tagatcgaga tacttttaca attgactata cagatgatga
     2521 attgctcgat aaagaagcag tttttactac tcattagaaa aataggtctg aataaattgg
     2581 tttcccttag aaccacatct aggggctttt atgtataata gaataataag tatcactcct
     2641 ttacaatacg tgtaacacgt gttataatgg tgttgtcagg aggtaaaagc gctatgccta
     2701 tgacacaaaa agagatggtt aaactcttta cggcccatgg ttggataaag actagaggcg
     2761 gtaaaggctc ccatattaaa atcgagaagc aaggggaaag acctatcaca atccttcatg
     2821 gtaagctgaa taagtacact gaaagaggga taggaaagca agctgggttg taaaattcag
     2881 tccctgcttt ttgatatgga ggtagtgaaa tgttagttac gtatccagct ttattttatt
     2941 atgacgacac agacggaaca gaagcgactt attttgtcca tttcccagat tttggatact
     3001 cagctacaca aggagagggg atttctgagg ctttggctat ggggtcggaa tggctaggga
     3061 taactgttgc agatttgata gagagtgatg gagagttacc tcagccatca gatatcaata
     3121 gcttgtcttt aattgataat gatcctttta aagatgatga agatttcgtg tcaacctatg
     3181 accttgataa atcttttatt tctatggtat ctgttgatgt atcagaatac ctaggaagtc
     3241 aggaacccat taaaaagact ttgaccatac caaaatgggc agataagttg ggacgagaaa
     3301 tgggacttaa cttttctcag actttgacag acgctattgc agataagaaa gttcaagcct
     3361 aagattaagc gtggtatcgt ggtaatagtc aataaaaaag cacgtccact tgtactagtt
     3421 actcgcctat tgattttata gatttattag ccctttcgag ggctttttat attgattttt
     3481 atgagaaatg aagaaatcag acttttaaga aaatcactga tatcaagggt ttaaatgagg
     3541 taatatggta taattaggac ataaaataat tttgtggtaa gatggtagta tctattttag
     3601 catatttccg agcaatcggg gcgattaaag agtcgcatag aaagaggaca aaatggcaac
     3661 atacggattt ttagatgttt tagaggaaga gttggacaag aactttccct ttgactttga
     3721 gattagctgg gacaaacgca accatgcggt agaagtgagt tttctattgg aagcacaaaa
     3781 tgctgcaggt gtggagatgg tggatgaaga cggagaggtt tcatcagatg acattctttt
     3841 tgaggaagcg gtccttttct acaatcctgc caagtcaaca gtcaatgagg aagactattt
     3901 gactgttatt ccttacctgc ctaaaaaagg attttcccgt gaatttttag cttattttgc
     3961 gcttttcctt aaagatactg ctgaggttgg gctagatgtc ctcatggact ttttggaaga
     4021 cccagaagca gaggaatttg tcatggaatg gaaccaagaa gtctttgaag aaggaaaagt
     4081 tggcttggaa gagggagaat tttatcctta tccaagatac taggagttgg ttggaggtgt
     4141 tatgaagaaa attgggactt atttggttta tgtgctagct gttgtcttta ttatgctagc
     4201 ttttgcttgt ggaacaatcg catttgcaga gttggggtat tccgcagttt tagcctttac
     4261 ttttggttat gccttcgctc ttctaagcat gtatttaatt tttattcttc atgagctggg
     4321 tcatgctttt tgtggctact tgacaggcta tcggctggtg gcttttggat taggacattt
     4381 tattttgacc aaaaagtcag gcaggtttca tcttagtaga acagccattc tgaaaaatgt
     4441 tggtgctcaa tacattggtt taaaagagga tgaaagcgat caaaaaatca tcctgatgct
     4501 ttcaggaggc ttgatggttc atctcggctt gatattattg gcgatagtgt ttggattttt
     4561 gacaagaagc tggtattttg cagggacttg gatttttctt aatttatctt tcttcctaaa
     4621 taacattttg ccagtcgaca tcaccgatgg agcaaaaatt tgggaattac tacaacaccc
     4681 tgaaaatacc aaatacgcct acctggtatt gagacattct gctcaaactt tgctggctcc
     4741 tcaagaatat gatttgaaag actttgttca agcagttcct gaggatgcac gagggagttt
     4801 tgcggatggt gtattaggta tgcaaggtga agtttccatt ttggaaggga aggaagaagt
     4861 agctaaacag cagtttcaag cattacttga gagggcaact actccgatga tgcagacagt
     4921 tgcccaacta tccctgcttc atatcgcctt gttggaagga gattttgagc aagcggagca
     4981 atatgctagt attcgacagg tcaaatcgtt tctgtctctc aaactgtcca atcttcaagt
     5041 gatacaagcc tggtatcaat taaaggtgaa gaaagatgta gatcgaactc gtaaggctct
     5101 caagattgct agacagaaaa tggatagtag tcgaatgttg agggacgaga aacgctatta
     5161 tgaaaacttg ttagccgagc tggaaaagga aatgttagaa ggaactgaag atggaactag
     5221 aaatttctgg tttcccaggc tgtaagattg ctttgttttg tggggataag cttttgacta
     5281 tcttacgcga tgataaggca agcattccct gggccaatat gtgggaactg ccaggtggtg
     5341 gaaagagtgt cagggaaaat ctgatatagt tttgcattaa aagaatatcc tttcaaggtt
     5401 attctttttt aattttgtcc caaaataatt gactttgtcc cgaaaatata gtattatttg
     5461 tataaggagg aatgaatcat gaaaaaatca gatgttcttg atttaataaa gtatcattat
     5521 gagggcagag agacagaatt tagaaatcaa tccatagcaa ttgctcggaa cttcaataaa
     5581 catggtgata cacaaatagc acaatatatt atggggttaa tgtctcaaag tgatagattc
     5641 atgccacaaa tagaaaatcc tagtgagtat ttgactcctg ctaaactaga tattggtcct
     5701 ctcccattgc ctctttcaat tatgaatgat ttaaaaggaa taataaacgc tgtcaatcat
     5761 catattggaa taaataaatt tttatttgtg ggatctccag gtactggaaa aactgaaagt
     5821 gtaaagcagg tggctagact tattggcaag gaattacttg tagtagattt cagtcactta
     5881 gtagatagta agctagggca aacagtcaaa aacttagcaa ctctttttaa tgaaatcaat
     5941 aatcttcctt tcaagcaaaa ttatatcatc ctatttgatg aaattgattc catagtgttg
     6001 gatagggtta atcagaatga tttaagagaa atgggacgag tgacttctgc ctttttaaag
     6061 gagttggata ggctgtcacc agaaattgta ttaattgcaa caacaaatct ttttgaaaat
     6121 cttgataaag cagttacgag aagattcgat gccataattg actttgaccg ttatactgat
     6181 gaagataaag tagaagtagc tactattatt ttaaatgagt tattaaagca atttaagaat
     6241 gtagctagag atttaaaatt atttaaaaaa attatcaata gtgccaatgt aataccgaat
     6301 cctggtgatt tgagaaactc aataagaacg tcattggcat ttagtgaccc atcagatccg
     6361 catgattacc aaaaacgcct gctaagaagt ttgcataatg gtagaaattt atctatttct
     6421 aaattatcga agctaggttt tactgttaga gaaattgaga ttttgacagg tatttctaag
     6481 agtagtgtat cacgagagtt aagcgaggat taaaatgaat acattattga cattacgagg
     6541 taaaagtttt acaccaaagt cagcaaaggg gaggggaccg ataacgattc ccgcagattc
     6601 tattgttacg ctaaaccatt taaatgaact ttatacatcc cttgaacgag taaaagaata
     6661 ttggtctaat aacacaatca ttgatggagt acttattagt gtgtattata atcgtgtcct
     6721 agctaagagc aatcgtatcg atggtttttt taatgtgggt ggcggaaatg caattccaaa
     6781 tgaaacgatt gtgggtgcaa aatttagtaa tgataagagt aggcatatta ttacacacta
     6841 catttcaaag gaagtgttag ataagacaat tgagcatact aaaaaagtta taacaatatt
     6901 caaagattat tttgctagtg ctcccatgac gagtatggtg ttctccgact cttcaaaatt
     6961 taatcaaatt gattattcta agtatggtct gtcgaaatca aaattcaaac aatacttaag
     7021 agacgcttgc tttgtcaaag gatttggtat tgaacaggca gatatgtcta ctatacgtaa
     7081 ttctattgta acaatctacg atgtaaaaac taatctctta acattactta aacaacttga
     7141 tattgatgtt tcagaagcta acatcatgga ccagacaact gttttacttg acgaaaaaaa
     7201 tattgagtta ctactgctaa aagcacctta tctagtatca atgatagtag aagatttttc
     7261 tagcctgtca ccagagaatt tttataatga tgggaacact ttttataata atctcccttt
     7321 tccaactaat gaacctatta ttggtgtcat cgatacgttg tttgatgatg gagtttattt
     7381 caatgagtgg gtagagtatc atgatttaat ttcaccagat attagcaaag gaagtcaaga
     7441 ctataagcac ggtacagcag tttcctcggt aatagtagat ggagctagtt tgaatccaaa
     7501 actagatgac ggatgtggaa gatttagagt cagacatttt gggatatctt tacagagtgg
     7561 tttcaattcc ttcacaatta tcaaacagat tagagaaatt gtgtctcaaa atcctgatat
     7621 taaggtttgg aacctttcgc ttggatcaaa tgatgaaatt agggaaaact ttatttccgt
     7681 cgagggttcg ttgttagacg agattcaatt tgataacgat gtaattttta ttattgcagg
     7741 cactaatggg attgcccctg atggacaacg aaaaaagatt ggagcaccag cagattcact
     7801 caattcaatt attgtaaatt ctgttgattt tagcaatcaa gtcgtttctt attcacgcga
     7861 aggagttgtc ctgtctttct ttatcaaacc tgatgtttca tactatggcg gggggaacgg
     7921 agacttcata aatgtttgtg aaccactagg gctggctcga gtagctggga ccagttttgc
     7981 ggctccattt attgctagaa aaatggctta cctcattcat attatgggat taagtcgtga
     8041 agaagcaaag gctttattaa ttgattcagc gataccatgg aatgataaaa aaacattcgc
     8101 agacatttct ttacttggat atggggttgt gccaatcaaa atagaagata ttttgtctac
     8161 acctgatgat gaaattaaat ttatagtctc agatgtttct agagcatatg aaacttataa
     8221 ttatgatttc ccagttccaa tttctaaaga acactatcca tatgtagcaa aggccactat
     8281 gtgctatttt ccaaagtgtt ctagaaatca aggagttgac tatactaata cggaaatgca
     8341 acttagtttg ggagttttag ataagaaagg cattaaaaca attaataacg acaatcaata
     8401 ttctgaaaat gcaccaggat atgtgagaga aaagtcggca cgaaatattt ttaggaaatg
     8461 ggataatgtc aaacatttag gagaaaaatt ttcttctcgt aaaaaagcta agccgattct
     8521 gaatccttct aatcctcaat ggggaatgag tgtaaaaaca attgaacggt taagaaatga
     8581 tgatggttat ggtattcgtt ttggagtagt tgttactcta aaggagttga atggtgtcaa
     8641 tagaatcgaa gattttattc aacaagccga attacgcggt tggctagtta atagattgca
     8701 agtagaagct caagtagacc tctttaatag tttaaatgaa gaaattgaat ttgaatagtg
     8761 gatattttat ccttattcta gatcttagga acacaagagg gcaaagatat gaatattact
     8821 tatatagttg gaaatggttt ggatttgcaa tatggtttga aaacaagata taaagatttc
     8881 tatgaatttc aaaataggac ttacataagt aggaaagaga acgaggaaga atattcaaat
     8941 ctcatttatg agtctctatt ttctgataca gtaaatgatt atgaaaattg gtcagatttt
     9001 gaattgtcaa ttggtaaatt aacaaaagat aacgacctca tctcctcaag tatagaaata
     9061 aaggagaaat ttatagatga ttttagtgaa gtagttgatg atttaagaga atatctacgc
     9121 atacaacaag aaaaaaatct tgagaaaggt aatgcaattg actttataag cactctagat
     9181 aatatgagga cctctctccc tgtaattaat caacctgcta tcgataaaaa atataatgaa
     9241 aatctatatc aacacgatat cgtgaatata ttaactctta attacacaaa tgtaatcgat
     9301 aaattatata atgagtcagc aaaatcattt agcaatcaat tacgaactaa taattataaa
     9361 tttcacattg cgcctccaat tcatgcacat ggaacggtag atatttgcac agttttagga
     9421 gttagtgacg agacacagat ttcaaatagt tttgatgaag aacaaaaaga atccttaata
     9481 aaaaatttag ttttgaagaa ctatagagaa aatatggatg ttaaaaatag tgatattata
     9541 aaaaattctg acatcattat tctctatggg gtttctttgg gagaaactga taggtatatc
     9601 tggagtcaga ttgctgaacg atcaatcagc ggttctgttc ctgtcataat ttaccactat
     9661 gtacctcatt ttgatccggg gaatcccatt agagcaaaac gcctatatag aaatgtagaa
     9721 gataaattca tacagaatag tggtatagac ttagagttag agaaaaaatt aagagacaac
     9781 cttattgtcg ttattggaaa gactattttt aatctgatgg aaaggtaagc aaaatacata
     9841 atggaaacat ggcaagagtt aaaagttaca gtgaagcgtg agggagagga gttggtttcc
     9901 aatctcttga ttgagctagg agcgcaaggt gttgcgattg aagacagtat ggactatgtg
     9961 ggaaatgttg accgctttgg cgagattttc ccagaggtgg agcaacagga agaaatcgtt
    10021 gtgacagcct actaccctga cacggtggat gtgacagtgg ttgaggcgga cttgcaggct
    10081 cgcatatcag aattgacgga ttttatggat ttgggagagt taaaaatagg tacgactgtc
    10141 ttggctgagg aagactgggc agataactgg aagaaatact atgagccagc tcgtatcact
    10201 catgatttga ccatcgttcc ctcttggaca gactatgagg cgactgcggg ggaaatgatt
    10261 atcaagctgg atcctggcat ggcttttggt actggaaccc acccaactac caagatgagc
    10321 ctttttgcct tggaacaggt tcttcgtggt ggcgaaacgg tgctagatgt ggggactggt
    10381 tcaggggttc tctctattgc cagctcgctt ctgggggcta aagaaatttt cgcctatgac
    10441 ctggatgatg tggcggttcg tgtggctcag gaaaatattg agctcaaccc tggcatggaa
    10501 aatatccatg tagcggctgg agatttgctt aagggtgtgg aaattgaggc agatgtgatt
    10561 gtggctaata tcttggcgga tatcctcatt catctaatag acgatgccta tcgtttggtt
    10621 aaggacgaag gctacctgat catgagtggc attatcaagg ataagtggga catggtgcgc
    10681 gagttggctg aatcagctgg atttttcctc gaaactcaca tggttcaagg ggaatggaat
    10741 gcctgtgtct ttaagaaaac caaggatatc tctggtgtga ttggaggcta gcatgcagca
    10801 gtattttgta aaaggcagtg ctatctctcc tgtcactatc gagaacaagg aaaccagtaa
    10861 gcatatgttt caggttatgc gcttgaaaga agaggatgag gttaccttag tctttgatga
    10921 tggcatcaag cgcttggcgc gcgtgctgga tatggaaaat cgtcagtttg agttggtcca
    10981 agaattaaat gacaatgtgg aactaccagt ccaagtgacc atcgcatctg gatttcccaa
    11041 gggagacaag ctggagttca tcgctcaaaa agtaaccgaa ctgggtgcta gccaaatctg
    11101 ggcctttcct gcagactggt cagttgccaa gtgggatggc aagaaattgg gtaaaaaagt
    11161 tgaaaaacta gaaaaaattg cccttggagc agccgagcaa agtaagcgta atgttgttcc
    11221 aagtatccat cttttcgaga ataaagcaga ctttctagcc caacttgacc agtttgaccg
    11281 catcttagta gcctatgaag aggcggccaa agaaggagaa gccgctgctc ttataaaagc
    11341 agttactgga ctcgagaaag gagccaaatt gctctttatc tttggtccag aaggtggtct
    11401 gtcacctgcg gaaattgaaa attttgaagc taagggagct gttttggcag gacttggccc
    11461 tcgcattcta agagcagaaa cagcaccgct ttacgcttta tcagccctta gtgttttatt
    11521 agaattagag aaataagagg aagaaaatgg aacaaaaaca ccgttcagaa tttccagaga
    11581 aggaactttg ggatttaaca gttctatacc aagaccgtga ggatttcttg cgtgcaatcg
    11641 agaaagctcg cgaagacatc aaccagttta gccgtgatta caagggcaat cttcacactt
    11701 ttgaggattt cgagaaggcc tttgcggaat tggaacagat ctacattcag atgagccata
    11761 ttggcaacta tgcttttatg cctcagacga cggactatag caatgacgaa tttgcaaata
    11821 ttgctcaaac tgggatggaa tttgaaacag atgccagcgt agccttgacc ttctttgacg
    11881 atgctttggt ggcagcagac gaggaagtct tggaccgttt gggtgaattg cctcacttga
    11941 cggcagctat tcgtcaggcc aaaatcaaaa aagcccacta cttaggggct gatgtggaga
    12001 aggccttgac caatctcggt gaagttttct acagtccgca ggacatttat actaagatgc
    12061 gagctgggga ttttgaaatg gctgactttg aagcccatgg caagacctac aaaaacagct
    12121 ttgtgaccta tgagaatttc taccaaaacc atgaggatgc tgaggttcgt gagaaatcct
    12181 tccgttcctt ctcagaggga cttcgtaagc accaaaatac ggctgcagca gcctatctgg
    12241 ctcaggtcaa gtctgaaaaa ctcttggctg atatgaaggg atacgactct gtctttgact
    12301 atcttctagc tgaacaagaa gtggaccgtg tcatgtttga ccgccagatt gacctcatca
    12361 tgaaggactt tgcaccagtc gctcagagat acctcaagca tgttgccaag gtaaatggtc
    12421 ttgaaaagat gacctttgca gactggaaat tggacttgga cagcgccctg aatcctgaag
    12481 tgactattga cgatgcctat gatttggtca tgaagtcggt agaacctttg gggcaagaat
    12541 attgtcagga agttgctcgt taccaagaag agcgctgggt ggactttgct gctaacagtg
    12601 gcaaggattc cggtggttat gcggcggacc catatcgcgt acacccttat gtactcatga
    12661 gctggacagg ccgtttgagc gatgtctata ccttgattca tgaaatcggg cattctggtc
    12721 aattcatctt ttcagacaat catcaaagtt acttcaatgc ccatatgtcg acctactatg
    12781 ttgaagcacc gtcaaccttc aatgaattgc tactcagtga ttacttagag aaccaatcta
    12841 atgacccacg tcaaaaacgc ttcgctctgg ctcatcgctt gacagacacc tacttccata
    12901 actttatcac ccacctcttg gaagcagcct tccagcgtaa ggtgtataca ttgattgaag
    12961 aaggggagac ctttggagca agcaagctca acagcattat gaaggaagtt ttgacggatt
    13021 tttggggcga tgccattgaa atcgacgacg atgcagcttt gacttggatg cgccaagctc
    13081 actactatat gggcttgtat agttacactt actcagcagg actagttatc tcgactgctg
    13141 gttaccttca tctaaaacat tctgaaactg gagctgaaga ctggctaaat ctccttaaat
    13201 caggtggtag caagacacca cttgagtcag ccatgattat cggtgcggat atttcaacag
    13261 acaaaccact ccgtgatacc atccaattct tgtctgacac agttgaccag attatcgcct
    13321 acagtgctga gttgggggag taaggggata gaaaaatttt ttagaatgat gtttatgggc
    13381 ttgttaagcg tcattctatt tgtcttagtt gtagctattt tcaaaaatgt cgttgaaggt
    13441 cgtgatgtta aatttgaact tactgtagaa ctggtattga tagttattta cgtcatttta
    13501 tatcagattg tatttaattg agaattcaat attgagtcat ttaagactgc ctttttgaac
    13561 agttgctaga aatgtagagg tattgaccat gtatcaagat ttactaagaa aaatagcaga
    13621 agaaaaacca aattataacc aggaagaaat ccagtggttg tttgatcatt tgggaaatcc
    13681 ttctccagaa attcgtgatg acctttcaaa tcaaggcttg cactatcttt caaaagaaaa
    13741 ggatacaaca ggtttctcaa gtcagtatgg ttgggttcac gcttttgcac atggagccga
    13801 tttactgaca gaggtggttt gtcatccaga ctttcctaaa aacagagttc atgaagtatt
    13861 tgatatactt ggccaactat ttaaaagaat gtcgattcgc tttacagatg atgaggattg
    13921 gcgtttagca agagtgatct atgaacctat tttataaggg aagttggagc aagaacaagt
    13981 agcttcttgg ataaaaactg ttgattttcc gatagaagaa agggaggatt tttacaaatt
    14041 ttccaacttc agatcctgtc tggtggaagt ctatgtccaa cttgaccaga gaaatagttt
    14101 acaagatgag ttgaaagaag ctatccagtc ttttcaatac tagagattag ctaatagttc
    14161 tatgattttc aaaaaatttc caatcaattt tcttgaaacc ctttcctact tttgatagac
    14221 tagtatctag tttttgaaaa aaggagaaag aaaatgaaaa aatttgttgc tgagttaatc
    14281 ggtacgttca tgcttgtgtt cgttgggaca ggagctgttg tttttggaaa tggtcttgat
    14341 ggccttggtc accttggaat cgcctttgcc tttggtttgg caatcgtggt ggcagcctac
    14401 tcaatcggaa ctgtttcagg tgctcacttg aacccggctg tttcgattgc tatgtttgta
    14461 aacaaacgtt tgtcatcttc agaacttgta aactacatcc ttggtcaggt tgttggagct
    14521 ttcatcgctt ctggcgcagt cttcttcctc ttggctaact caggtatgtc aactgctagc
    14581 cttggtgaaa atgccttggc aaacggtgtc actgtctttg gtggtttctt gtttgaagtc
    14641 atcgcaactt tcttgtttgt attggttatc atgactgtga cttcagaaag taagggcaat
    14701 ggcgcgattg ctggtttggt aatcggtttg tcattgatgg cgatgattct tgtcggattg
    14761 aagattactg gactttcagt aaacccagct cgtagcttgg caccagctgt cttggtaggc
    14821 ggcgcagccc ttcaacaagt ttggattttc atccttgcac caatcgctgg tggagttctt
    14881 gcagcccttg ttgcaaaaaa tttccttgga acagaagaat aattgaaact caaaaagcct
    14941 tgctcctcat cttgaggaac agggcttttt cgtatgatac tcttcgaaaa tctcttcaaa
    15001 ccacgtcagt ttcaccttgc cgtaggtatg gttactgact tcgtcagttc tatctgcaac
    15061 ctcaaaacag tgttttaagc aacctgcggc taacttccta gtttgctctt tgattttcat
    15121 tgagtatgac tttagcggtt gtcaattttc tctggataaa ggtcgtgttg gaagaggcgt
    15181 tgttctgcca agccctcata cttagttcct tgcttaccgt agttgtagta ggggtcgatt
    15241 gaaatgccac cgcgcggagt gaattttccc cagacttcta aatagcgagg gtctagcaag
    15301 ttgaccaagt ctttcccgat ggtgttgata cagttctcgt ggaaatctcc gtggtttcgg
    15361 tagctaaata gatatagttt gagggatttt gactcgacac agagcttgtc aggaatgtag
    15421 gaaatatgaa tcgtcgcaaa gtctggctga gcagtgattt gtcccagcag agacatatcg
    15481 aggatatggt gacgaatgcc ctgttcctta gcgatttctc tagtaatttg aatttcgagg
    15541 tgatgacgtt ggccgtaggc aaaggtgaca gcttcgactg tttcatagtg ttgcatggcc
    15601 cagaaaaggc aggttgttga atcttgacca ccactaaaga cgaccaaggc tgattgatgt
    15661 ttcatagtac tccttccaaa atgggaaatg ttcagagcac gcaaaaagct cccattaggg
    15721 agctaaaaaa taccaaatcg agttttttta gcgatggcat atcccaaaca tcgtaatatt
    15781 ctacttatat agtaaaatga aataagaaca ggacaaattg atcaggacag tcaaatcgat
    15841 ttctaacaat gttttagaag tagaggtgta ctattctagt ttcaatctac tatagtctag
    15901 catatttttt gaaaaatggc aaagggcaag aaaaaagaga ccaaagaaag tacttggtct
    15961 ctcgtttgat tagctcaatt cagcaatgat ggccttgatt tgttctgctg tgtgaacacc
    16021 tgcaacttgt ttgacaactt ggccgtcttt tttgaagaga agagttggaa tagacatgat
    16081 tccaaaagca cgagctgtgt ttggattttc atcaacgtcc attttaacga ttttcaagac
    16141 atcttctgaa agttcttcag acaatttgtc caagattgga ccttgcatac gacatggacc
    16201 acaccaagtt gcccagaagt ctactaagac caaaccgtct tttgtttctt gttcgaatgt
    16261 tgcatctgta attgcttttg ccattgtatt tctccttttt ttagttatat tggcttaaat
    16321 cttgtttcat gagatagaag aagatatctc cataagtccc atggtagtcc aaattatgcc
    16381 ccttgtaggt taatttttgg acagggtagt agtctgcgac gccgataagg caagcttgtt
    16441 gcgaacgttc aaagtcttca taagactcga aagtcacagt tctttcgttc ttgctggcat
    16501 ctatataggt aatttcaatc atatttaaaa ctcctttgtt taatgctaac tttattttac
    16561 tccttataaa agagaatgtc aagaaaaatg attgcgcacg caactttttt taaaatcatc
    16621 ttaaatcaag aaatccaaac ctgcttccaa gctttcttcg acagtctttt gtagcgaggc
    16681 cagtgtcttt tgcccatcat ttgtcaggca gataaaacta gagcgtctat cttgatggca
    16741 acacatgcga ctgagtagac cgcaattttt agcttccaag cgagccacca tcctagaaac
    16801 tgcgctcggg ctcagatgaa gcttatctgg caggtcaatc tggcgtagag atttttcttc
    16861 agccaagtcc agatagtaga gcaggtagaa ctctttcaag gtcagacttt gctcgctctg
    16921 ttgggcaatg gtctcttcca agagactttc aatttctttc tgacgccgat tgaagtcaaa
    16981 ccatttttcc aaataggtca tagtgtctcc tttcttttta gagtcataaa atagaagaaa
    17041 gtccattaac gggcagtctc tgcgtcacaa gatgattgcg catgcaataa ttatactact
    17101 tttcaagaat gctggcaagc tctgtttttt agtggtttta tttttgtgtg aataatgggg
    17161 aaatcctatt gtttcaattt ctaactcctt atcacattcg aattcagatt ttatttcatt
    17221 tctctatcta tagttgctta gtttaaaata agcatggtct aataaagcta tgcatatagt
    17281 actgatttta aacagcaagc attagattcc atcaaagagg ggcacagaca tgtcgaggcg
    17341 gccaaagttt ttgatgttga cgccagaact ctcttcacgt gggaaaagaa agacatgaac
    17401 aaaggaactt agagcggaaa aagcgagtcg tcaaaaagcg taagatccct ttagaagaat
    17461 tgaaagcctt tgtagaggct catccagacg cttttttacg ggaaattgcg gcccgttttg
    17521 attgtgcttt gccctccgta tgggcagttt taaagcagat taaggtcatt ttaaaaaaga
    17581 cggccagttt tagggaacaa aagcctgaga aagtttctga gtttcttgat attttggata
    17641 acctaaaaga tttaccagtc ctatatattg acgaaacggg aatcaaccgc tacctctatc
    17701 gtccttatgc aggggctcct agaggggaga aagtctatga caagattagc ggacggcgtt
    17761 ttgagcggac taatgaggtg gagcaaaaac tcaatggtag ttttctaatc agatatattg
    17821 attcacaaat tagagaatga c
//
LOCUS       DAVKGC010000033        17591 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKGC010000033 DAVKGC010000000
VERSION     DAVKGC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.033"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            15..1325
                     /locus_tag="VW049_000923"
     CDS             15..1325
                     /locus_tag="VW049_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9432074.1"
                     /translation="MLVSSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGAL
                     DTYHPIAYRFAFIVVMISILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFV
                     SLGVFLLTYPFYFIRLFERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYL
                     ATKPKKLQATAVLVSFIAANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFK
                     EKLAIFVGSPILMLAMGVLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFN
                     SSLPYRDFRNFTFGPVLDYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLV
                     LNKEYLKGHGIGSSYIMELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSL
                     LILNNLFFMPRSSFSESFFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            1354..2388
                     /locus_tag="VW049_000924"
     CDS             1354..2388
                     /locus_tag="VW049_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9432075.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            2588..3325
                     /gene="mecA"
                     /locus_tag="VW049_000925"
     CDS             2588..3325
                     /gene="mecA"
                     /locus_tag="VW049_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9432076.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            3476..4762
                     /locus_tag="VW049_000926"
     CDS             3476..4762
                     /locus_tag="VW049_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9432077.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            4764..5633
                     /locus_tag="VW049_000927"
     CDS             4764..5633
                     /locus_tag="VW049_000927"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9432078.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            5709..5786
                     /locus_tag="VW049_000928"
                     /pseudo
     CDS             5709..5786
                     /locus_tag="VW049_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            5928..6866
                     /gene="msrB"
                     /locus_tag="VW049_000929"
     CDS             5928..6866
                     /gene="msrB"
                     /locus_tag="VW049_000929"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9432079.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            6924..8648
                     /locus_tag="VW049_000930"
     CDS             6924..8648
                     /locus_tag="VW049_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432080.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLSKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            8650..10398
                     /locus_tag="VW049_000931"
     CDS             8650..10398
                     /locus_tag="VW049_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432081.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGRVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKRAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQFPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            10790..12049
                     /locus_tag="VW049_000932"
     CDS             10790..12049
                     /locus_tag="VW049_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9432082.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPSGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    12125..12260
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            12299..12799
                     /locus_tag="VW049_000933"
     CDS             12299..12799
                     /locus_tag="VW049_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9432083.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            12875..13243
                     /gene="rplL"
                     /locus_tag="VW049_000934"
     CDS             12875..13243
                     /gene="rplL"
                     /locus_tag="VW049_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9432084.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            13430..13600
                     /locus_tag="VW049_000935"
     CDS             13430..13600
                     /locus_tag="VW049_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432085.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            13600..13848
                     /locus_tag="VW049_000936"
                     /pseudo
     CDS             13600..13848
                     /locus_tag="VW049_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            13862..14202
                     /locus_tag="VW049_000937"
                     /pseudo
     CDS             13862..14202
                     /locus_tag="VW049_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14195..14575
                     /locus_tag="VW049_000938"
     CDS             14195..14575
                     /locus_tag="VW049_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9432086.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            14588..14731
                     /locus_tag="VW049_000939"
     CDS             14588..14731
                     /locus_tag="VW049_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432087.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            14824..15411
                     /locus_tag="VW049_000940"
     CDS             14824..15411
                     /locus_tag="VW049_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9432088.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            15613..15750
                     /locus_tag="VW049_000941"
                     /pseudo
     CDS             15613..15750
                     /locus_tag="VW049_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
     gene            16040..17443
                     /locus_tag="VW049_000942"
     CDS             16040..17443
                     /locus_tag="VW049_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU9432089.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGP
                     YILSDKRWDEAPIYLRYGVFRPSRDDEKKVVIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSEMPKIYWYGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKAFLLVN"
BASE COUNT         5372 a         3106 c         3767 g         5346 t
ORIGIN      
        1 ctactttatt gttcttgttg gttagtagct taattgcttg ttggaatgat atcaagtatt
       61 acttaatcca ttttattttc tatttaacca tttttgtatt tctggtatca agaccgacca
      121 ttgattattt tagggatggt gctttggata cctatcatcc aatagcctat cgttttgcct
      181 ttatagttgt catgatttcg attctgggct tgaccacagg aggcattctg gctcgttact
      241 tcatagctag gaagaaaata aaagtagcaa atataggaaa ttctctaaaa gaggtttata
      301 tcaagcggtt acgctttgta tcactaggag tttttcttct aacttatcct ttctatttca
      361 ttcggttatt tgaacggctc ttgtatcgtt tgcagacttc ctactatgcc tactatgcaa
      421 attttgaaag taaactgcct tattttacct acattttgtc tacctttacg gtctatgcaa
      481 tgtgtatgta tctggcaacc aagccaaaga aattgcaggc cacagcagtg cttgtctcct
      541 ttattgcagc taatactatt catttggcaa ttgggacacg aaatcccttt attttaagta
      601 ttttatttgc ttttgtttat tactttatgc gggagcaaac tgaaaaagga aaatggattg
      661 ggtttaaaga aaagttagcg atttttgtag gttctcctat tctcatgtta gcgatgggag
      721 tactcaatta tgtacgggat aatgtccaag tttcccatac aggtttctgg gatatcttac
      781 ttgactttat ctataaacaa gggactagtt ttggtgttct ggctcgaggt tttctattta
      841 acagtagcct cccttaccga gatttccgta attttacttt tggtcctgtt cttgattatt
      901 ttgcaagggg gagtttggga gccattttcg gaggaaaagc ctttgaacat acaaccaata
      961 gtgtggaact agctattgat agtaatagtt atgcccacaa tctatcctat cttgtcttga
     1021 acaaggaata cttgaaaggg catggtatcg gaagtagtta tatcatggag ttgtataccg
     1081 actatggtat gattggagtc tttctgctta gtttcttact cggcgtatta tttatagcca
     1141 tgctgcaagt agcctatcgc tcaaggacaa tcctatttgc tttatcccta ctcatcttga
     1201 ataatctatt ctttatgcca agaagcagct tttcagaaag tttcttcaat ttatttacaa
     1261 tgcaattctg gggaattgtt cttgtgatta tatttgtagc aaaaatgctt acaaaggaaa
     1321 actagtatct actaaacaaa ggagaaaaaa atcatgtttg aacattattc agtagctgat
     1381 ttgtttgcaa atctttataa aaagcgaaaa gcaaatattt tagctctcat cgctttattt
     1441 gctctcattg ctgtaccatt tacaattaaa gcagttagga ataaaaacac tgtcaaagat
     1501 acaacaagtt attcaactta tcttatctat aaaatcactc ctccaaaaga gtcggccaaa
     1561 acgattttga atcatcaaat tggtggttat agtgattttt atgggaaatt gattgatggt
     1621 aatttgaatg gagcttatct tttcaatgat gtagaaccca gtgagttgaa aaaaattgcc
     1681 agtgaattag acacgacaga aacaaccttg aaaaattcaa cgaatgacta ttggtggaaa
     1741 aaattgaccg tctactatat gattgacgat gcaggggttg gtgtgaaaat tttgacatca
     1801 agtaaagatg ccaatgactt gttagagaaa aaaattgatg ggttgattga gaaatttaaa
     1861 catgcctatg caaatgtgaa aattgaaaaa ctggaaacca tcaactctaa agaattgaac
     1921 gcaaatggtg aaacagcgct tggcttaaat gtgaaaaatc tgattcttcg tttagttgtt
     1981 attggagtgg tttgtgtgat tttggttgtg atgggaaatg tgttagttta tctctttaat
     2041 ccaacaatca atagagtagg tgatttttct cagtatcaaa ttgattttgt aacagagatt
     2101 acaacaattg ctaacctagc agatgttttg tcatacaaaa atactggaca ggaattgacc
     2161 atcgttagct caaataaagc tatcttagat aaattgaaac agagtcaaga agctttaaaa
     2221 ggaatgcatt ttgtagattt acaggatgta tcatctcttt tggaaagaga tacagtcctt
     2281 cttgttgaag agtacggagt gactcgttat aagaaatttg agcaaagtct tcaaattctc
     2341 agaaacttaa atcgttctat cctcggtgta gcaaccttta aattataagt attctgatgt
     2401 ataaggtctt caaaatcggc tcttagccga ttttgaaggc tttttctatc tgtctgcaga
     2461 aattttcttt tggagtctcc tatagaaaga ccctagggaa aatctatccg tatagaggag
     2521 ccttcttttg ataaaatcgt aaaaatctag tataatagat agaactgaaa gtatgaggtt
     2581 actagcaatg aaaatgaaac aaattagtga tacaacttta aaaatcacga tgtctttaga
     2641 ggatttgatg gatcgtggaa tggagattgc tgactttctc gttcctcaag aaaaaacaga
     2701 agagttcttt tatgctatct tggatgagct agagatgcct gatagctttc tggatacagg
     2761 catgttgagc ttccgtgtga ctccaaaacc tgataaggta gatgtctttg tgaccaagtc
     2821 aaagattgac caaaatctag attttgaaga cttatcggat ttgccagata tggaagaatt
     2881 ggctcaaatg tctccagatg aatttatcaa aaccctggaa aaaagcatcg cagacaaaac
     2941 caaggatgat atcgaagcca ttcaatctct tgagcaagtt gaagccaagg aagaagagca
     3001 agagcaagct gaacaagaag ctgagagtaa gaaagaacct tacatctact acatcctttc
     3061 ttttgctaag ttggctgact tggtagcttt tgccaagaca gtgacttttg aaatggaaac
     3121 ttctgaactc tacaaaatga acgagcgcta ttatttgacc attttagtgg atattgaaaa
     3181 tcatccaagc ccatatccag cttggctgtt ggcccgtatg cgcgagtttg cagacgatag
     3241 tgatatcagt cgctcagtct tacaagagta tggtcaagtc ttgatgagtc acgatgcagt
     3301 gctcaatctg caaaaaatcg gctaatcctt tctggaaagc tattttcaga ttttaagaag
     3361 agcgaatcgt ctgattcgtt ttttcttttt atactgaaat agtgatttac tataatagga
     3421 attttcacaa aattctgtta taatggctat attagaaaat ttcgaggaga taaagatgac
     3481 agttaaaatt gctttactag gatttggtac cgttgcaagt ggtgtgcctt tcctcctaaa
     3541 ggaaaatgga ggaaaaatca atcaatcagc acattcagat atcaaagttg ctaaggtatt
     3601 ggtcaaggat gaagatgaaa aaaatcgctt gcttgcagca gggaatgact ttaactttgt
     3661 aaccaatgtg gatgatattt tatcagacca ggatattact atcgtagtgg aattgatggg
     3721 gcgtattgag cctgctaaaa cctttatcac tcgtgccttg gaagctggaa aacacgttgt
     3781 tactgctaac aaggaccttt tagctgtcca tggcgcagaa ttgctagaaa tcgctcaagc
     3841 taacaaggta gcactttact acgaagcagc agttgctggt gggattccaa ttcttcgtac
     3901 tttagcaaat tccttggctt ctgataaaat tacgcgcgtg cttggagtag tcaacggaac
     3961 ttccaacttc atggtgacca agatggtgga agaaggctgg tcttacgatg atgctcttgc
     4021 ggaagcacaa cgtctaggat ttgcagaaag cgatccgacg aatgacgtag atgggattga
     4081 tgcagcctac aagatggtta ttttgagcca atttgccttt ggcatgaaga ttgcctttga
     4141 tgatgtagcc cacaagggaa tccgcaatat cacaccagaa gacgtagctg tagctcaaga
     4201 gcttggttac gtagtgaaat tggttggttc tattgaggaa acttcttcag gtattgctgc
     4261 agaagtgact ccaaccttcc tacctaaagc gcacccactt gctagtgtga atggcgtaat
     4321 gaacgctgtc tttgtagaat ctatcggtat tggtgagtct atgtactacg gaccaggtgc
     4381 tggtcaaaaa ccaactgcaa caagtgttgt agctgatatt gtccgtatcg ttcgtcgttt
     4441 gaatgatggt actattggca aagacttcaa cgaatatagc cgtgacttgg tcttggcaaa
     4501 tcctgaagat gtcaaagcaa actactattt ctcaatcttg gctctagact caaaaggtca
     4561 ggtcttgaag ttggctgaaa tcttcaatgc tcaagatatt tcctttaagc aaatccttca
     4621 agatggcaaa gagggtgaca aggcgcgtgt cgttatcatc acacacaaga ttaataaagc
     4681 ccagcttgaa aatgtctcag ctgaattgaa gaaggtttca gaattcgacc ttttgaatac
     4741 cttcaaggtg ctaggagaat aagatgaaga ttattgtacc tgcaaccagt gccaatatcg
     4801 ggccaggttt tgactcggtc ggtgtagctg taaccaagta tcttcaaatt gaggtctgcg
     4861 aagaacgaga tgagtggctg attgaacacc agattggcaa atggattcca catgatgagc
     4921 gtaatctctt gctcaaaatc gctttgcaaa ttgtaccaga cttgcaacca agacgcttga
     4981 aaatgaccag tgatgtccct ttggcgcgcg gtttgggttc ttccagctcg gttatcgttg
     5041 ctgggattga actagccaac caactgggtc aactcaactt atcagaccat gaaaaattgc
     5101 agttagcgac caagattgaa gggcatcctg acaatgtggc tccagccatt tatggtaatc
     5161 tcgttattgc aagttctgtt gaagggcaag tctctgctat cgtagcagac tttccagagt
     5221 gtgattttct agcttacatt ccaaactatg aattacgtac tcgcgacagc cgtagtgtct
     5281 tgcctaaaaa attatcttat aaggaagctg ttgctgcaag ttctatcgcc aatgtagcgg
     5341 ttgctgcctt gttggcagga gacatggtga ccgctgggca agcaatcgag ggagacctct
     5401 tccatgagcg ctatcgtcag gacttggtaa gagaatttgc gatgattaag caagtgacca
     5461 aagaaaatgg ggcctatgca acctaccttt ctggtgctgg gccgacagtt atggttctgg
     5521 cttctcatga caagatgcca acaattaagg cagaattgga aaagcaacct ttcaaaggaa
     5581 aactgcatga cttgagagtt gatacccaag gtgtccgtgt agaagcaaaa taaagaatag
     5641 aagataggat ggggaaactc ttgaccagag ggtttcatat cctttttgtg aaaagaagtt
     5701 tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgc tcaaaacagt
     5761 gttttgaggt tgtggataga actgacgacg tcagctcaag acactgtttt gaggttgtgg
     5821 atagaactga cgaagtcagt aacatatata cggtaaggtg acgctgacgt ggtttgaaga
     5881 gattttcgaa gagtattagt taaaaacgtg ataaaggaga aataaagatg gcagaaattt
     5941 atctagcagg tggttgtttt tggggcctag aggaatattt ttcacgcatt tctggagtgc
     6001 tagaaaccag tgttggctac gctaatggtc aagtcgaaac gaccaattac cagttgatca
     6061 aggaaacaga ccatgcagaa acggtccaag tgatttacga tgagaaggaa gtgtcactca
     6121 gagagatttt actttattat ttccgagtta tcgatcctct atctatcaat caacaaggga
     6181 atgaccgtgg tcgccaatat cgaactggga tttattatca ggatgaagca gatttgccag
     6241 ctatctacac agtggtacag gagcaggaac gcatgctggg tcgaaagatt gcagtagaag
     6301 tggagcaatt acgccactac atcctggctg aagactacca ccaagattat ctcaggaaga
     6361 atccttcagg ttactgtcat atcgatgtga ccgatgctga taagccattg attgatgcag
     6421 caaactatga aaagcctagt caagaggtgt tgaaggccag tctatctgaa gagtcttatc
     6481 gtgtcacaca agaagctgct acagaggctc catttaccaa tgcctatgac caaacctttg
     6541 aagaggggat ttatgtagat attacgacag gtgagccact cttttttgcc aaggataagt
     6601 ttgcttcagg ttgtggttgg ccaagtttta gccgtccgat ttccaaagag ttgattcatt
     6661 attacaagga tctgagccat ggaatggagc gaattgaagt tcgttctcgt tcaggcagtg
     6721 ctcacttggg tcatgttttc acagatggac cgcgggagtt aggcggcctc cgttactgta
     6781 tcaattctgc ttctttacgc tttgtggcca aggatgagat ggaaaaagca ggatatggct
     6841 atctattgcc ttacttaaac aaataaaaca gagagtgggg cttcccactt tcttcatttc
     6901 tagaatatga atagaaggga tttatgaaac acctattatc ttacttcaaa ccctacatca
     6961 aggaatcaat tttagccccc ttgttcaagc tgttagaagc tgtttttgag ctcttggttc
     7021 ccatggtgat tgctgggatt gttgaccaat ctttacctca gggagatcaa ggtcatctct
     7081 ggatgcagat tggcctgctc cttatctttg cagtaattgg cgttttagtg gccttgatag
     7141 ctcaatttta ctcagcaaag gcagcagtag gctttgctaa ggaattgaca aacgatcttt
     7201 atcgtcatat tctttccttg tccaaggaca gcagagaccg tctgacaact tctagtttgg
     7261 tcactcgctt gacttcggat acctaccaga ttcagactgg tatcaatcaa ttcctgcgtc
     7321 tctttttgcg agcgcccatt atcgtttttg gtgccatttt tatggcttat cgaatctcag
     7381 ctgagttgac tttctggttc ttagtcatgg ttgccatttt gaccattgtc attgtagggt
     7441 tatctcgatt ggtcaatcct ctctacagta gtctcagaaa gaaaacggac caactggttc
     7501 aggaaacgcg ccagcaattg caagggatgc gggttattcg tgcttttggt caagaaaaac
     7561 gagagttaca gatttttcaa acccttaacc aagtttatgc tagattacaa gaaaagacag
     7621 gtttctggtc tagtttatta acacctctga cctatctgat tgtcaatgga actcttctcg
     7681 ttattatctg gcaaggctat atttcaattc aaggaggagt gctcagtcaa ggtgctctca
     7741 ttgctcttat caattacctc ttacagattt tggtggaatt ggtcaagctt gccatgctga
     7801 tcaattccct caaccagtcc tatatctcag ccaagagaat cgaggaggtc tttgcggaat
     7861 ctcccgaaaa catccattca gaattagaac aaaagcaagt taccagtggt cgggttttac
     7921 aagtccaaga attgactttt acctatcctg atgcggccca gccttctctg agagacattt
     7981 cctttgatat gactcaagga caaatccttg gtatcattgg ggggactggt tctggtaaat
     8041 caagcttggt gcaactctta cttggacttt atccagtaga caaggggaac attgaccttt
     8101 atcaaaatgg acgtagtcct cttaatttgg agcagtggcg gtcttggatt gcctatgtac
     8161 ctcaaaaggt cgaactcttt aaaggaacca ttcgttccaa cttgactcta ggtttcaatc
     8221 aagaagtatc tgaccaggaa ctctggcagg ccttggagat tgcgcaagct aaggattttg
     8281 tcagtgaaaa ggaaggactc ttggatgctc tagttgaggc aggggggcga aatttctcag
     8341 gtggacaaaa acaaagattg tctatcgccc gagcagtctt gcgccaagct ccgtttctca
     8401 tcctagatga tgcaacctcg gcactggata ccattacaga gtccaagctc ttgaaagcta
     8461 ttagagaaaa ttttccaaac acgagcttaa ttttgatctc tcaacgaacc tcaactttac
     8521 agatggcgga ccagattctc ctcttggaaa aaggtgagtt gctagctgtt ggcaagcacg
     8581 atgacttgat gaaatccagc caagtctatt gtgaaatcaa tgcatcccaa catggaaagg
     8641 aggactagaa tgaaacgaca aactgtaaac cagacgctca aacgtttagc cgtagattta
     8701 gcaagccatc ctttcctcct tttcctagcc tttctaggaa ctattgccca agttggctta
     8761 tcaatttacc tacctattct gattgggcgg gtcattgacc aagtcctagt ggctggttca
     8821 tcaccagttt tttggcagat ttttctccag atgctcttgg tggtaatagg aaatactcta
     8881 gtacaatggg ccaatcctct cctctataat cgtctaatct tctcttatac cagagattta
     8941 cgggagcgaa tcatccataa actccatcgg ttaccgattg cctttgtaga taggcaaggt
     9001 agtggagaga tggttagtcg tgtaaccaca gacatcgaac agttggcagc tggcttgacc
     9061 atgattttta accaattttt cattggtgtt ttgatgattt tggttagtat tctagccatg
     9121 ctccaaattc atctcctcat gactctctta gtcttgctgt tgacgccact gtccatggtg
     9181 atttcacgct ttattgccaa gagatcctat catctcttcc agaagcaaac agagacgagg
     9241 gggattcaga cccagttgat tgaagaatcg cttagccagc agactataat ccagtccttc
     9301 aatgctcaaa cagagtttat ccaaagattg cgtgaggctc atgacaacta ctcaggctat
     9361 tctcagtcag ccatctttta ttcttcaacg gtcaatcctt cgactcgctt tgtaaatgca
     9421 ctcatttatg cccttttagc tggagtagga gcttatcgta tcatgatggg ttcaaccttg
     9481 accgtcggtc gtttagtgac ttttttgaac tatgttcagc aatacaccaa gccctttaac
     9541 gatatttctt cagtgctagc tgagttgcaa agtgctctgg cttgcgtaga gcgtatctat
     9601 ggagtcttag atagccctga agtggctgaa acaggtaagg aagtcttgac cagtgaccaa
     9661 gttaagagag ctatttcctt taaacatgtc tcttttggct accatcctga aaaaattttg
     9721 attaaggact tgtctatcga tattccagct ggtagtaagg tagccatcgt tggtccgaca
     9781 ggtgctggaa aatcaactct tatcaatctc cttatgcgtt tttatcccat tagctcggga
     9841 gatatcttgc tggatgggca atccatttat gattatacac gagtatcatt gagacagcag
     9901 tttggtatgg tgcttcaaga aacctggctc acacaaggga ccattcatga taatattgcc
     9961 tttggcaatc ctgaagccag tcgagagcaa gtaattgctg ctgccaaagc agctaatgca
    10021 gactttttca tccaacagtt tccacaggga tacgatacca agttggaaaa tgctggagaa
    10081 tctctctctg tcggccaagc tcagctcttg accatagccc gagtctttct ggctattcca
    10141 aagattctta tcttagacga ggcaacttct tccattgata cacggacaga agtgctggta
    10201 caggatgcct ttgcaaaact catgaagggc cgcacaagct tcatcattgc tcaccgtttg
    10261 tcaaccattc aggatgcgga tttaattctt gtcttagtag atggtgatat tgttgaatat
    10321 ggtaaccatc aagaactcat ggatagaaag ggtaagtatt accaaatgca aaaagctgcg
    10381 gcttttagtt ctgaataagc gattctcttt tgaaagttta tggacgaaaa aagttgcctt
    10441 cgagtgactt ttttgttaca atagctagaa aaattgttca ctgtaatact caatgaaaat
    10501 caaagagcaa actaggaagc tagccgtagg ttgctcaaag cacagctttg aggttgtaga
    10561 taagactgac gaagtcagct caaaacactg ttttgaggtt gcagatagaa ctgacgaagt
    10621 cagctcaaaa cactgttttg aggttgcaga tagaactgac gaagtcagct caaaacactg
    10681 ttttgaggtt gcagatagaa ctgacgaagt cagtaaccac acctacggca aagtgaatct
    10741 gaagtggttt gaagagagta caacttgtct tttagaaaag gagcctacaa tgaaagtctt
    10801 tcagcatgta aatatcgtga cttgtgatca aaatttccat gtttatcttg atggaatctt
    10861 agcagtcaag gattctcaaa tcgtctatgt cggtcaagat aagccagcgt ttttagaaca
    10921 agctgagcag attatagact atcagggagc ttggattatg cctggtttgg tcaattgtca
    10981 cacccattct gcaatgacag gtctgagagg gatccgagat gacagcaatc tccatgaatg
    11041 gctcaatgac tatatctggc cagcagaatc tgagtttact cccgacatga ctaccaatgc
    11101 ggtcaaagaa gccttgacag agatgctcca gtcaggaaca acaaccttta acgatatgta
    11161 taatcccagt ggtgtggata tccagcaaat ttatcaggtg gtgaaaactt ccaagatgcg
    11221 ttgttatttt tctccgactc tcttttcttc agagacagaa acaactgctg agactataag
    11281 cagaactcga tccatcatag atgaaatctt aaaatataaa aatccaaatt tcaaggttat
    11341 ggtagcacct cattctccgt atagctgcag tagagacttg ctggaagcga gtttggaaat
    11401 ggcaaaagag ctaaatattc cgctccatgt ccatgtggcg gagaccaagg aggagtcagg
    11461 aattatcctc aaacgatacg gcaaacgccc ccttgctttt cttgaagaac tgggttattt
    11521 agatcatccg tccgtatttg ctcacggggt cgaattaaac gagagagaaa ttgaacgctt
    11581 ggcatcttct caagtggcta tcgcccacaa tcctatcagt aacctcaaac tggcatcagg
    11641 aattgctcca attatccagc tccaaaaagc gggagtagca gtcggaattg cgactgactc
    11701 ggttgcttcc aataacaatc tagatatgtt tgaggaagga aggactgcag ctcttcttca
    11761 gaagatgaaa agtggggatg ccagccagtt tccaatcgaa acagctctca aggtactgac
    11821 aatcgaaggg gctaaggtcc ttggaatgga aaatcagata ggaagtctgg aagtcggcaa
    11881 gcaagcagat tttctggtca ttcaaccaca agggaaaatt catctccaac ctcaggaaaa
    11941 tatgctgtct cacctggttt atgcagttaa atctagtgat gtagatgatg tttatatcgc
    12001 cggagaacag gttgttaagc aaggtcaagt cctgacagta gaactttaaa agaaaaatta
    12061 cgaaaaattt taaaaaaagt ttgcaaaaat cttgcattct ttttttgact atgctatact
    12121 tatatacggt ttaaaaaaac tgcctaagac agtaggggag ctcgactcat aaatatccta
    12181 ccgaggacaa aacgtatcat gtaaaaagaa gcgtattgta ctttcgtgtc taggtttggg
    12241 cgcgtttttc tttttgaaaa attccccaag caaaataatt acggaggtga acacactaat
    12301 gagtgaagca attattgcta aaaaagcgga actagttgac gtagtagctg aaaaaatgaa
    12361 agctgctgca tctatcgtcg ttgtagacgc tcgtggtttg acagttgagc aagatacagt
    12421 tcttcgtcgt gagcttcgtg gaagcgaagt tgagtataaa gttattaaaa actcaatctt
    12481 gcgtcgtgca gctgaaaaag ctggtcttga agatcttgca tctgtatttg ttggaccatc
    12541 tgcagtagca ttttctaatg aagatgttat cgcaccagcg aaaatcttga acgacttttc
    12601 taaaaacgct gaagcacttg aaattaaagg tggtgcaatc gaaggcgctg tcgcatctaa
    12661 agaagagatt cttgcacttg caactcttcc aaaccgcgaa ggacttcttt ctatgctcct
    12721 ttctgtactt caagcgccag tgcgcaacgt tgctcttgca gtcaaagcgg ttgcagaaag
    12781 caaagaagac gcggcttaat cttaagctac acagcgtagc ctagctacga aaaaaactat
    12841 tataaaattt aaaacttatt tggaggaaat aacaatggca ttgaacattg aaaacattat
    12901 tgctgaaatt aaagaagctt caatccttga attgaacgac cttgtaaaag ctatcgaaga
    12961 agaatttggt gtaactgcag ctgctcctgt agctgttgct gcagctgatg cagctgatgc
    13021 tggtgctgct aaagattcat tcgacgttga attgacatct gcaggcgaca aaaaagttgg
    13081 cgttatcaaa gttgtacgtg aaatcactgg tcttggtctt aaagaagcta aagaacttgt
    13141 tgacggtgca ccagcacttg ttaaagaagg cgttgcaact gcagaagctg aagaaatcaa
    13201 agctaaattg gaagaagctg gagcttcagt tactcttaaa taatagagta tcgcaatttg
    13261 attgcggaat ccctatttac cagtctttga gagcgataag cgattgatag aaactgataa
    13321 attgatttgg tttcctgcca aaaattcttt ggcaggattt ttgttttaat aaactttcgg
    13381 gggtagacac tagattgtct accccttttt ttgtgtttcg gaggtaatta tgcaaaagga
    13441 aggacagtca tcacgtcaat tcgtgaagag tcatctcatg actatcatgg gagttgatat
    13501 tgtaaaagca actcagttag ttgatgaaat ggaacaagta ggtttaatcc gatttgatga
    13561 atttggaaat gttggaattt tagtcttgga gggacaataa tgaagcgtat taccgctaat
    13621 caatatcaga catcagagcg ttattataag ctccccaaac ttttatttga gagtgaacgg
    13681 tataagaata tgaaactgga ggttaaggtg gtttactcag ttttaaaaga tcggttggag
    13741 ttgtctttga gcaaaggttg gattgatgag gatggggcta tttatttgat ttattccaat
    13801 tcaaatttga tggcactttt aggctgttca aagtcaaaat tactctccat gtgagtttga
    13861 agtgacattt ttagatgatt accataaaaa acataactac ccactatttt acgaatccta
    13921 tcttcaaaac gttatggaat tccttgaaag tcaagacata aagaatgggg ttgatgcctt
    13981 tgtagatgat catcaaaatc tcgtttttgt tttatatgga caaggctatc gagccgaggg
    14041 aaaagaggga atacttacaa cccaagtaac tgtaaaagct tatgatgaag acaagaaacc
    14101 gattaacttc gcaaatttat tagattcctt aatttattag attccttaat cgtgtcagaa
    14161 tatcaaatgg aaccgaatct ttgggaggtc tcccatgatt gatctctatc taagtaaaaa
    14221 tagacaaaga aatcaacttc ttttagattt cttccaaaac tatggcatag aggtatcttg
    14281 tcattcagtt tctgaaatga caaaggacaa attaattgag atgatgagct attcttcaga
    14341 ttgttttgag tttttatctc ccaatttatt acgatttaag aaccgtgaca atctaagatt
    14401 aacggatttc gttgagataa ttttaaaaaa tcctgaacta accatcagac ttcctcttgc
    14461 ggtttcaaat aagcgagttt atccaagtct gaatttggaa gaggctagag ctctattgcc
    14521 aagagatacg aaacaattga tttacatggc gcaaacacat tatttatcta actaaaggag
    14581 aagcaatatg gctgaacgat tttgggagaa cttgtccatt attttggctg aaagaaatat
    14641 cagctggatt gagttaacca gaaaaatgtt tgcgggagag tttcactatc caagtgaatt
    14701 gaatcgtttg tatcaaaaga ttcgccacta gaagatggaa caatgaatgc ctcaaagtcc
    14761 atgggtagaa aggattgtgc aggtattaga tttagattat gaagatttat ttcggaggta
    14821 actatgaaaa gaataattcc agtttatata ttccaacaag taaatgtcct attggtatct
    14881 ctatacttat tgaaatttct ttgtatcggt gagttaacta tactacagat tctctatggt
    14941 tcgtcactca tttctttttt atggatgtat ggccaacgca aacaagcgca taaggtcaat
    15001 atgaaatcta ggatgaaatg gcttggtgtt gaatttgtta gcctactgat tataagtcta
    15061 tgttttagtc tgattcatgc tcaaggaagc acgaatcaag caaacttaat tggccttcaa
    15121 catcaaattc cttggttttc atttttattg ttcttaatca atgcgagtat ggttgaagaa
    15181 tttctgtatc gagaaatttt atggaatttg gtcagaaaat tagatatcag agttgctttg
    15241 acaagtgttt tatttgcctt agcacatcat acaggaacca ttctagcttg gtgtttgtat
    15301 gtttcacttg ggatgttttt aggtatggtg cgctataaat ctgacttatg gggcagtatg
    15361 ggactacatt tggtgtggaa cctactagtt tatagtttgc tgctttttta aaataggtga
    15421 cctgattttt aggtaacttc taaaactaac ttccagtttt gggagagaga tggaagttac
    15481 tttgaaaagt tacggaaaca ttattttcta tgtgacaaca ttgtatcatt gcttgcattt
    15541 tactgctgga gagagtataa tgaaggtgga taaagcactt aatcctaaaa atttaaatag
    15601 gaggagttag ctatggcaga agaagtatta aatctttaat tagtatctgt tcaagttgac
    15661 gaaacagatg aagttgatgg aatgcgattt agtacattca gtacaaaccg ttgcggtaac
    15721 tggagtgctt ttagttggga aaattgttaa ttcatttaaa ttgtaaactt tagagattct
    15781 ctggagttat ggatctcgtt ccaatatgtg cacgttggaa tgtcagtgct tatatggggg
    15841 gaatccaaag gattctccct ttttatttta ataagtctgt ctagctatag atatattaag
    15901 tttgatagaa tttcttggag attttgttat ggattataat ttcaatttgg agcatccttt
    15961 ctttttcact aataatgatt atagtacaga tactagcata aagtactaag taagtctgcc
    16021 ttttaattgg cacgaagtaa tgaataatga tgagtgggtt tatcaatacc ctattggaaa
    16081 atttgttgaa agacaagggt ggaaaataca cattagttca gagtataata gttcacacga
    16141 attgttacaa gatgtagcaa aaatttgcca tgaaatgcga attccattta aacatcttag
    16201 tacagaagac aaatttatca tgcgaaacgg gaaattggtt agtcgaggat tttctggtaa
    16261 atttattaca tgttatccaa atcaaaatga attagagtcg gttttgcaaa ggttagaaag
    16321 tgccttaaag caatataatg ggccatatat tttaagcgat aaacgttggg atgaagcacc
    16381 aatatatctg aggtatggtg tgtttaggcc gtcaagggat gatgaaaaga aggttgttat
    16441 tgatgaatta attgttggcg atgaagttgt taaagatgaa cgtttaccag tatttaaaat
    16501 tccaaaaggt atagtgcccc ctgacttttt gaataaatgg cttgacaaga aagataaaaa
    16561 acagggagac tttcccttta ttatagataa tgcaattaga ttttcaaaca gtggtgggat
    16621 atataatgct agactaaaag aagatgggaa aaaaataatt ttaaaagagg ctagaccgta
    16681 tacaggtcta ggatttgatg gtacttattc ttcagagaga ttagcttcgg agtgcaaagc
    16741 actaaaaatt ctaaatgaat ggagtgagat gcctaaaata tactggtacg gtaagatctg
    16801 ggagcacacc ttcttaggaa ttgaacatat gaagggcgtt ccactaaata gatgggttac
    16861 caataatttt cctttatatg aagttgttga taagaccaaa gattatctat tgagagtttc
    16921 aaagatagta gaaaaactca tcgatttaac gaataagttt cattctgaaa atgtatatca
    16981 tcaagattta catttaggaa atatcttggt taaggatgaa gatgagatta gtataataga
    17041 ctgggaacag gctgtctttt cgaatgatga aaaagttgta cataaagttg cagcccctgg
    17101 ttttagagca tggcgagaaa cacttcccag tgagatagat tggtatggta ttagacaaat
    17161 agctcattat ttatacatgc cactagtaac tacatcggat ttgacataca actatgtaag
    17221 tcaaaccagg atagaaggaa aaaaactatt tgagtctttg ggttatacaa gagaacatat
    17281 agattatgta gaaagtttat tgtcatatct agattctaaa tgtccacaga ttgagaatat
    17341 ttccaggaaa aaagttttaa aacccatgca tgagattaga actatcgaga gtgagcaaga
    17401 tatacaagat tttattataa aagcttttct tttagtgaac tgataggtat tttcttagca
    17461 aacgataaag ttttagggcc gatacaatca attgcctatt cattaaataa gataaataca
    17521 accaaagatt taaggaaacc gtttttaaaa tacttaagtg gagagaagaa ttttatagac
    17581 gctgaacatg a
//
LOCUS       DAVKGC010000034        17194 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKGC010000034 DAVKGC010000000
VERSION     DAVKGC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17194
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.034"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(349..2607)
                     /gene="glgP"
                     /locus_tag="VW049_000943"
     CDS             complement(349..2607)
                     /gene="glgP"
                     /locus_tag="VW049_000943"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU9432090.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGENSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2633..4150)
                     /gene="malQ"
                     /locus_tag="VW049_000944"
     CDS             complement(2633..4150)
                     /gene="malQ"
                     /locus_tag="VW049_000944"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9432091.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            4700..5971
                     /locus_tag="VW049_000945"
     CDS             4700..5971
                     /locus_tag="VW049_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9432092.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6082..7374
                     /locus_tag="VW049_000946"
     CDS             6082..7374
                     /locus_tag="VW049_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9432093.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            7376..8218
                     /locus_tag="VW049_000947"
     CDS             7376..8218
                     /locus_tag="VW049_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9432094.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(9343..9753)
                     /locus_tag="VW049_000948"
     CDS             complement(9343..9753)
                     /locus_tag="VW049_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HEU9432095.1"
                     /translation="MYELSNRDLDGIDIELGRYRTLANKIYLRRQELIHNKKHSTEDY
                     TGGKGKTVSSPTEATIIRIEEDQTLRYLEGFKLVVDTLMENLIESDLVIFKMRYLEAG
                     ATWEDVAEKLNKTTRYINSRRKVIAKRFVELKGY"
     gene            complement(10136..10687)
                     /locus_tag="VW049_000949"
     CDS             complement(10136..10687)
                     /locus_tag="VW049_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432096.1"
                     /translation="MDIREVLSILENLDDKKDKIAKARTKLEEKRKTITGEKKVSFDN
                     IDSFFENNATSLEQITKMSESIDLLEKEYDTYFWEAKAAIFEYIFKETKRRAEEKKIY
                     KRYQKKLRIILDAYDEIQSLKKDVEEIHKGVVREITQKHSLAVYRTEVNPTSILPFLN
                     PDISGHMNFSKEYHEIKEYLGKE"
     gene            complement(10698..11549)
                     /locus_tag="VW049_000950"
     CDS             complement(10698..11549)
                     /locus_tag="VW049_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEU9432097.1"
                     /translation="MAKKFSLVEKYVRSRGMSSDDGKSKTDLILSQDITSIYDVPEEG
                     KELVDLVNVIEHTGTGGTYETVGFYDEHLSELESEDFRDSKSVELRKKQIKTKFEHKT
                     FSGRIPLSSEQVDDGEYNISDFLSNKITRLCRKTRNIEIGKILKEAPEKNVSNFDELK
                     DTINDLNPERHNTIVLSQSLFKFLDKEKSSDGNYILKINKKERFSENLYVDDVIVVSD
                     EVLGVKGDKVAFVGDLYNFATLFERNKNSLRWVSESVIYGMSLMLYTRFVVKKIETDC
                     AFFIKWN"
     gene            complement(11586..11936)
                     /locus_tag="VW049_000951"
     CDS             complement(11586..11936)
                     /locus_tag="VW049_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU9432098.1"
                     /translation="MSLTSDLANEIAKTLEAYSEEVEEQIDLIAEEVTNEAVNELKQT
                     SPKRYGKYAKNWRFKKNSKGSFVIYNADPTYRLTHLLEHGHVLRNGGRSKAIPHIKPV
                     EEKIKEKFEQKIKK"
     gene            complement(11994..12215)
                     /locus_tag="VW049_000952"
     CDS             complement(11994..12215)
                     /locus_tag="VW049_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432099.1"
                     /translation="MSGDTSLGYVVANKFSMDPDKRQKIFSQCKKEGDSLEQRKQEIL
                     EKYANKQDKPKSRKNDSKGSESHKRKAKN"
     gene            complement(12305..12460)
                     /locus_tag="VW049_000953"
     CDS             complement(12305..12460)
                     /locus_tag="VW049_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432100.1"
                     /translation="MDEMKFSTEKGFIVYEKCGIIEIEKIPRFGEITLVYSDGKFTHL
                     VKKETKK"
     gene            complement(12466..13311)
                     /locus_tag="VW049_000954"
     CDS             complement(12466..13311)
                     /locus_tag="VW049_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9432101.1"
                     /translation="MKEQFKEFNNKKISDKVCDIHQVNYWEISIPVVGSSERKIQPFC
                     PECVKGDIKQKEQDLLQRFDDRQTYFKTYDVLMRDSTIPNELKGATFDNFFVKTTEEG
                     QMLEFVKGQAQKYLAGMTGNTLISGSTGIGKSHLSLALAKEINESFREKNDPKSVLFV
                     SLTEIIKQIKEGWAYGRNANLTEYEAVKKLVDVDFLIIDDLGAKNGTVTPKSDWEQDF
                     LFDIINNRETTIFNTNLDSSELRTVYNARNSSRILKGLEGNTFKAFTIKDKRYTINTV
                     RGEKG"
     gene            complement(13323..14183)
                     /locus_tag="VW049_000955"
     CDS             complement(13323..14183)
                     /locus_tag="VW049_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HEU9432102.1"
                     /translation="MAKTKIYFWLKVDKKFFDNLFIKRLKNMPGGYTMTVIYIRLMLE
                     SLEDDCILYYEGYFDSLVQELALKLDVSEDDINMTVAYFTKCGLIQIDDDGHATLSQA
                     KAMVESETNWAKYKREQRKNSQDLPKLENVQNKKTISNSCPTEIEIENRVNSKSNNLY
                     LDNILSGNPDLTFPTWLEETAIKDLERTKHKELWVPIAYLNQVANKRYKFVDKTKRLL
                     LARFKEGYTLEDFKQVIDIKTAEWKDSPEFSKYLRPETLFGSKFDGYLNQKPKTIKGK
                     SEDNFPDLPF"
     gene            complement(14170..14451)
                     /locus_tag="VW049_000956"
     CDS             complement(14170..14451)
                     /locus_tag="VW049_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432103.1"
                     /translation="MFSLSRESEKDLTHGILEVVEKYLEAHEKVPPRLTKLINRVELK
                     EELKISDNTLNKWESQGLRRYQPPDDGSRMIYYLVTDIWKFLGVCDGKD"
     gene            complement(14444..14785)
                     /locus_tag="VW049_000957"
     CDS             complement(14444..14785)
                     /locus_tag="VW049_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9432104.1"
                     /translation="MGLSENDKRVLRLIKVGAENSITGLEISLTTKLTERTVRDIIKR
                     LVVKHNIPIVGVRCGVFSGYFIPANKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            complement(14766..15060)
                     /locus_tag="VW049_000958"
                     /pseudo
     CDS             complement(14766..15060)
                     /locus_tag="VW049_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122738.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15057..15271)
                     /locus_tag="VW049_000959"
                     /pseudo
     CDS             complement(15057..15271)
                     /locus_tag="VW049_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15268..15471)
                     /locus_tag="VW049_000960"
     CDS             complement(15268..15471)
                     /locus_tag="VW049_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432105.1"
                     /translation="MVKEKFDILTDYEELCGRLKDILIVLGMTSVGEDSEKTATVNVV
                     SQALESLIVEHTKNTHKYREDIV"
     gene            complement(15794..16054)
                     /locus_tag="VW049_000961"
     CDS             complement(15794..16054)
                     /locus_tag="VW049_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432106.1"
                     /translation="MENEFKTVTNAKGLEIPKYPKDFKKLVEKDRQLAEYLCMNYENL
                     DNEDLGAFLETVEQGFSWILDLIESKDLLYKPKSGSNHAKRK"
     gene            complement(16066..16668)
                     /locus_tag="VW049_000962"
     CDS             complement(16066..16668)
                     /locus_tag="VW049_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEU9432107.1"
                     /translation="MELVYMDGKKEPYTLSSIVADCAEIKHRHLKILLNKHREDFESF
                     GKVQFKISPSKSGQNVRDYILNEQQATLMITYLRNTEPVKEFKKNLVKAFFEMRDELS
                     KRYLQRELEKPKRKTLTEAIKSWEKAPKHAYSTLTNLLLKGATGKNKAQLMQERESEN
                     GIDSLTSAELTNYQRLEDMAIAMINLNREYSEIKELIFKA"
     gene            complement(16899..17096)
                     /locus_tag="VW049_000963"
     CDS             complement(16899..17096)
                     /locus_tag="VW049_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9432108.1"
                     /translation="MKNNMRLLLAKQRKKTADVAEATGISKSTLTALYYERAKNPSLD
                     TLKKVSSYLGVTLDEFLDTKE"
BASE COUNT         4828 a         3652 c         2807 g         5907 t
ORIGIN      
        1 ttctttccta caattatatt ataatacttt atgtaagcga atacaagaga tatgaggtaa
       61 ccaactacga aaaaatataa ggctgaagac agtaaaaacc agcatttaaa accttcactg
      121 tatctaggat ttccctattt ttagacaaga ttgacccaaa tccacagcat ttaaaaaagc
      181 gaatttcaat cgaaactcgc ttttttacaa aatgtattag tataaatttg tatcttatac
      241 tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gttgctcaaa gcaccgcttt
      301 gaggttgcag ataaagctga cgtggtttga agagattttc gaagagtatt agttcaagtg
      361 ccagatgtct tcgttatact gagcgattgt acggtcagat gagaagaatc ctgctttaga
      421 aatgttaacg atgactttat ccaaccatgc gtcacggtct tcgtagtcag caagcatttg
      481 ctctttgact ttgatgtagt cttccaaatc aagaagagtc atgaaccagt ctttgttgat
      541 caattcattg taaagacgtt ccaagcgctc tttgtttcca gctgcaagaa ctgcatcact
      601 aacgatgaag tcaaccaatg gtttgatagc ttcacgagcg tagaattcgc ttgatttgta
      661 agctgctttt gcgtaaaggt cgataacagt ttctgaattt tcaccgaaga tgtagatgtt
      721 ttcttctcca accaactcag cgatttccac gttagcaccg tccatagtac caagtgtcaa
      781 agctccgttc aacatgaatt tcatgttacc agtacctgaa gcttctttag aagcaagtga
      841 gatttgttct gagatatcac atgctgggat aaggaaactt gctgcagtaa cgttgtagtt
      901 ttcaaccata actacttgca agtgtggagc tactgctgga tcgttagcaa taacttctga
      961 catgcaaagg attaaatgga taatgtcttg agcgattgtg taggctggag ctgctttacc
     1021 accaaagaag attgtgattg gacgagcagg gatgttacca gctttgatgt caaggtattt
     1081 gtggatcacg tacaaagcgt tcatttgttg gcgtttgtac tcgtgaagac gtttgatttg
     1141 gatatcaaag atagaatttg aattgatttc cacaccttgg tgttctttca agtgacgagc
     1201 caatttacgt ttgttgtgag ccttgatgct ttccaatttt tctttgacag ctgctttgtc
     1261 ttcataagac aacagttttt caagctcatc tgcttcatgg tgccaaccat ctccaagaat
     1321 ctcatccaag tagtgagaca atcttgggtt agcatgcata agccaacgac ggaaagtgat
     1381 accgtttgtt ttgttgttga acttttctgg gtaaaggtcg tagaaggctt tcaactcaga
     1441 atttttcaag atttcagtat gaagtgctgc aaccccgtta acactgtatc cgtagtggat
     1501 atccatgtga gccatgtgaa cacgtccgct ctcatcgatg atttgaacag ctggatcttt
     1561 gtactctgcc ttcacacgac ggtccaattc ttcgatgatt ggtaccaagt gaggaaccac
     1621 ttcttgcaag aattcaagag gccatttttc aagcgcctca gcaaggattg tgtggttagt
     1681 gtaggcagtc atgctacgaa caattgatat tgcttcgtca agctcgatac cacgtgcagt
     1741 caaaagacga atcaattcag gaatcaccat tgatgggtga gtatcgttga tttggacaac
     1801 tgcgtagtca gcaaggtcat gcaagttgct tcctttttcg attgcttcgt cgatgatcaa
     1861 ttgcgcaccg tttgaaacca tgaagtattg ttggaagata cggagcaatt caccttgacg
     1921 gtcactatca tctgggtaaa ggaagagagt caagttgcga gcgatatctg tcttgtcaaa
     1981 gttgatacca tctttaataa tagaagaatc aactgaatcc aagtcaaaca aacgcaagcg
     2041 gtttttagtc gctgtttcat aaccagtaac atcaatatcg taaagagttg atgtcaaagt
     2101 aaagtctgca aatggtactt ggtagctacg gcttgagcga accaaccagt tttgctctgt
     2161 caaccatgca tttggaattg tttcttgttg gttgttttta agaacttgtt ggaaaagacc
     2221 aaagtggtaa ttaagaccaa caccgtcacc attcaaacca agagtagcaa ttgagtcgat
     2281 aaagcaggca gccaaacgtc ccaaaccacc attaccaaga gatggttcca attcaacttc
     2341 ttcaacttcg atcaagtctt tacctgcagc tgcaagttct tttttaacat cgtcgtaaag
     2401 accaaggtta atcaagttgt ttgacaagag tttaccaatc aagaactcag ctgagatgta
     2461 gtaaactttt ttcttaccag tgttgactgg tttttggctg cttgcaagct tgctgtagtt
     2521 aagaagagca aggtaaagct cttcattgct acattctgca atggttttat tgtaacgatt
     2581 ttgtacaaat tcttgtagtg ataacatgtt tagttgtctc ctgattattg tcttatttct
     2641 ttaaatctac caaattttca ttaattcggc gataaattgt tgtcaagtca agcaaacctt
     2701 cctcgacagc tggtgtcaat tgatcttcag tcatacgcca agaccagttt ccaccaaggg
     2761 tagatgggaa gttcatacga gctgcctcat ccaattctag taaatcttgc atagttgcaa
     2821 ttgccataaa gctaactgat gaaaatactg tacgaagcat agcgtgtacc actgtttcgt
     2881 attctttacg gttcgtgtaa cgagccatgt actcacgagt cgcatcatca atctcattac
     2941 ggtaccaacc aagaaccgta ttgttatcgt gtgttcctgt gtacataact gagttagcag
     3001 gtgccaagtg tgggctatca atgctttcgt cttctgggtt gaaggcaaat tgaagaatct
     3061 tcattcctgg gaagccagta cgttcacgca attcgatcac ttcatctgtc atgaagccaa
     3121 ggtcttctgc gatgatgttt agctcaccaa gttcttcctt aacggctgca aaaagcttgt
     3181 agcctggacc tttcacccac tcaccaggtg ctgctgtatc ggaaccagca gggatttccc
     3241 agtaagattc gaagccacgg aagtggtcga tacgaacgat atcgtagatt ttgaagcttt
     3301 cacgcaagcg ttcaatccac catttgtagc cgtctttgtc cattgcttcc cagtcataga
     3361 ttgggttacc ccaaagctga ccagttacag aaaactcatc tggtgggcat cctgcgatac
     3421 aagtagcctt accattgaca tctgttttga agagatgtgg atttgcccac atatcacttg
     3481 aatcttccgc tacgtagatt ggcatgtccc caacgatttc gatgtggttg tcgttagcgt
     3541 aagctttcaa tttcaaccat tgttggaaga agaagtattg agtcacacgg tggtaaacca
     3601 acttgtctgc caattgctca cgatagcttt caagtgctga agctttacga gcacgagcat
     3661 ctgcatctgg ccattcagtc caagcaagat tgtcaaaata ctctttgata gccatatact
     3721 cagcaaagag ctcaagccat gattggttgt cttgagcaaa tttctcaaaa tctttaacat
     3781 ctccgacttc aaagaaacgt ttcaccgctt tttctaaaag aggacgacgt gcatagtaga
     3841 ttttagcata gtcaacttca gacgcatcgc taccaaagtc aactccttca aggtcacttg
     3901 cttccaacaa accttgctcc accaatatat ctaaatcgat aaaatgagtg tttcctgcga
     3961 aggctgagaa agattggtaa ggagaatccc cgtaactagt tgctcctaat ggaaggattt
     4021 gccagtaacg ttgttttgta cggaccaaga aatcaacgaa gtcgtaagca ctttgaccaa
     4081 atgatccgat tccgtaagct cctggaagag aagagatgtg catcaacaca ccactttgac
     4141 gttttttcat aatagcacct cgtgtgttaa aataatggaa cgttgcgtat tttgcagacg
     4201 caaacgtttg cgttacttat aagtatactc cttttcaagc ataattgcaa gcgtttttaa
     4261 aaaacttttt tgattatttt taaggagact aactcatttt agcaaagata acttaaaact
     4321 atctaatcct aaagtttgac tctcacgaga cagtctacaa agtacaaaac ctccttaggg
     4381 agcttgaatt tatatgcaac aaagcacaaa cgtctataga aaatagatag ggattagaaa
     4441 cagggaggta gcccctcctg atttccctct ttagacagat tccatatatt tttattaaaa
     4501 atactgtatt cttatctatt tcatactttt cttctattaa aatgcaaaat tatgctatca
     4561 aatcaagctt aaaaattttt taaaattttt acaaaaaata cttgcaaccg ttttctattt
     4621 gtgctatact aagctcataa aggaaaacgt ttgcgtttcc ttatcaataa aacttgctat
     4681 tctttgggag gaatacacta tgtcatctaa atttatgaag agcactgcgg tgcttggaac
     4741 tgctacactt gctagcttgc ttttggtagc ttgcggaagc aaaactgctg ataagcctgc
     4801 tgattctggt tcatctgaag tcaaagaact cactgtatat gtagacgagg gatataagag
     4861 ctatattgaa gaggttgcta aagcttatga aaaagaagct ggagtaaaag tcactcttaa
     4921 aactggtgat gctctaggag gtcttgataa actttctctt gacaaccaat ctggtaatgt
     4981 ccctgatgtt atgatggctc catacgaccg tgtaggtagc cttggttctg acggacaact
     5041 ttcagaagtg aaattgagcg atggtgctaa aacagacgac acaactaaat ctcttgtaac
     5101 agctgctaat ggtaaagttt acggtgctcc tgccgttatc gagtcacttg ttatgtacta
     5161 caacaaagac ttggtgaaag atgctccaaa aacatttgct gacttggaaa accttgctaa
     5221 agatagcaaa tacgcattcg ctggtgaaga tggtaaaact actgccttcc tagctgactg
     5281 gacaaacttc tactatacat atggacttct tgccggtaac ggtgcttacg tctttggcca
     5341 aaacggtaaa gacgctaaag acatcggtct tgcaaacgac ggttctatcg caggtatcaa
     5401 ctacgctaaa tcttggtacg aaaaatggcc taaaggtatg caagatacag aaggtgctgg
     5461 aaacttaatc caaactcaat tccaagaagg taaaacagct gctatcatcg acggaccttg
     5521 gaaagctcaa gcctttaaag atgctaaagt aaactacgga gttgcaacta tcccaactct
     5581 tccaaatgga aaagaatatg ctgcattcgg tggtggtaaa gcttgggtca ttcctcaagc
     5641 cgttaagaac cttgaagctt ctcaaaaatt tgtagacttc cttgttgcaa ctgaacaaca
     5701 aaaagtatta tatgataaga ctaacgaaat cccagctaat actgaggctc gttcatacgc
     5761 tgaaggtaaa aacgatgagt tgacaacagc tgttatcaaa cagttcaaga acactcaacc
     5821 actgccaaac atctctcaaa tgtctgcagt ttgggatcca gcgaaaaata tgctctttga
     5881 tgctgtaagt ggtcaaaaag atgctaaaac agctgctaac gatgctgtaa cattgatcaa
     5941 agaaacaatc aaacaaaaat ttggtgaata aaaaatttgt tcaagggggg tggaaatcaa
     6001 atcccccttt gaatttatca atagagacac aaataattta gctttcttat aaaaaagtag
     6061 tatcctatga aaggagttaa tatggaaaag caacaaccta gtaaagcagc cctgctgtct
     6121 atcattcctg ggttaggaca gatttacaat aaacaaaaag ccaaaggttt tatcttcctt
     6181 ggtgtaacta tcgtatttgt cctttacttc ctagcacttg caacccctga attgagcaac
     6241 ctcatcactc ttggtgacaa accaggtcgt gataattccc tctttatgct gattcgtggt
     6301 gccttccatc taatctttgt aatcgtttat gtactctttt atttctcaaa tatcaaagat
     6361 gcacatacga ttgcaaaacg cattaacaat ggaattccag ttccacgcac actcaaagac
     6421 atgatcaaag ggatttatga aaatggcttc ccttacctct tgatcattcc atcttatgtt
     6481 gccatgacct tcgcgattat cttcccagtt atcgtaacct tgatgatcgc ctttaccaac
     6541 tacgacttcc aacacttgcc accaaacaag ttgttggact gggttggttt gaccaacttt
     6601 acaaacattt ggagcttgag taccttccgt tctgcctttg gttctgttct ttcttggact
     6661 atcatttggg ctttggcagc ttctacttta caaatcgtaa ttggtatctt cacagctatc
     6721 attgccaacc aaccatttat caaaggaaaa cgtatctttg gtgttatttt ccttcttcct
     6781 tgggctgtcc cagccttcat cactatcttg acattctcaa atatgtttaa cgatagtgtc
     6841 ggtgctatca acactcaagt attgccaatc ttggctaaat tccttccttt ccttgatgga
     6901 gctcttattc cttggaaaac agacccaact tggactaaga ttgccttgat tatgatgcaa
     6961 ggttggctcg gattcccata catctacgtt ttgactttgg gtatcttgca atctattcct
     7021 aacgaccttt acgaagcagc ttatattgac ggtgccaacg cttggcaaaa attccgcaac
     7081 atcactttcc caatgatttt ggctgttgcg gcacctactt tgattagcca atacaccttc
     7141 aactttaaca acttctctat catgtacctc ttcaatggtg gaggacctgg tagtgtcgga
     7201 ggtggagctg gttcaaccga tatcttgatc tcatggatct accgtttgac aacaggtaca
     7261 tctcctcaat actcaatggc ggcagctgtt accttgatta tctctatcat tgtcatctca
     7321 atctctatga tcgcattcaa gaaactacac gcatttgata tggaggacgt ctaagatgaa
     7381 taactcaatt aaactcaaac gtagactgac tcaaagcctt acttaccttt acctgattgg
     7441 tctatcaatt gtaattatct atccactgtt gattaccatt atgtcagcct ttaaagcagg
     7501 taacgtctca gcctttaaac tagatactaa tatcgacctc aattttgata actttaaagg
     7561 cctcttcact gaaaccttgt acggtacttg gtacctcaac actttgatta tcgccttaat
     7621 taccatggct gttcaaacaa gtatcatcgt acttgctggt tatgcttaca gccgttacaa
     7681 cttcttggct cgtaaacaaa gtttggtctt cttcttgatc atccaaatgg tgccaactat
     7741 ggccgctttg acagccttct tcgttatggc gcttatgttg aacgccctta accacaactg
     7801 gttcctcatc ttcctctacg ttggtggtgg tatcccgatg aatgcttggc tcatgaaagg
     7861 ctacttcgat acagtgccaa tgtctttaga cgaatctgca aaactagacg gtgcaggaca
     7921 cttccgccgc ttctggcaaa ttgttctacc acttgttcgc ccaatggttg ccgtacaagc
     7981 tctctgggcc ttcatgggac ctttcgggga ctacatcctc tctagtttct tgcttcgtga
     8041 gaaagaatac tttactgttg ccgtaggtct ccaaaccttc gttaacaatg cgaaaaactt
     8101 gaagattgcc tacttctcag caggtgctat cctcatcgcc cttccaatct gtattctctt
     8161 cttcttccta caaaagaact ttgtttcagg acttacaagt ggtggcgaca agggataatt
     8221 tatccccgcc accctttttc attttatact cttcgaaaat ctcttcaaac cacgtcagct
     8281 ttatctccaa cctcaaagtt gtgctttgag caacctgtgg ctagtttgca ctttgatttt
     8341 cattgattat tagcaattgt cactgtaaat aatatccttg tagcaagcaa tttttctcct
     8401 agacttgaaa taaagcgcat ttctctataa tttgacttag gcatcatttc acacctcaat
     8461 atagcgccta aacccttgat attactgact ttttgaaaaa tctcatttga ttagaaatca
     8521 ttaaaagttc ttaaaaattc tcaaaaagtc cacaaaaaaa aagccccaca aaagggcaat
     8581 aaatgacgag ttcagcaggc aagaaactag cacggtcaaa cgtgcttttt taatacctgg
     8641 taatattata gcatatcctc tcaaacttac atataaccag aatcctgcaa aagaaaaaga
     8701 taaaagcata gaggatagag cgaaaaccgt tcttactttt attttgtcaa atagcaatat
     8761 gagaagcaag gataaaaagc cctcagaaat aaatctaagg acttaaataa cgacgtgttg
     8821 gtttgctcac ttaattagtc tacttaatac tattcaatta tagtttactt tgaaatgttc
     8881 ttaatacttt ctgtaaattt ttgaatcgca ttcagttctg caggtcttaa atgaggctac
     8941 gctttagtcc tccccccatt tcgtttaata tgtccaaatt caagtgaatg agtaagagag
     9001 ccgtatttaa aactatatac gacccaaact ccatttctca cccttttctt acgccatcct
     9061 tttgaatatt tacctgctct tttagggcta gtttccctta attctttaac tgtttcttta
     9121 gcgacttgct ccgcaatttt atcaacttct tcattaacct cttcgcacta ttctgctaaa
     9181 atatttgcta attgtgctgc taaatccatt ttctaaaaac tccttctatt tttttaaaat
     9241 atcaactttt tacccccttt ttgtctggag gtctccgact tggaaaaagt tcccttcacc
     9301 ggtaccctac tggccagaaa gattttttaa aaggtggggg agtcaatatc ctttcaattc
     9361 cacaaatctt ttagcgatta cctttctacg gctatttata taacgagtag ttttatttag
     9421 tttctctgcc acgtcttccc aagtcgcacc agcttctaaa tatctcattt taaaaattac
     9481 tagatcactt tcaattaagt tttccatcaa ggtatctaca actagtttga aaccttctaa
     9541 atatcttagt gtttggtctt cttcaattct aatgattgtc gcttcagtag gactagatac
     9601 tgtcttgcct ttcccaccag tataatcttc agtgctatgt ttcttattat gtatcagttc
     9661 ctgtcttctc aaataaattt tattagcaag cgttctatat cgtcctaact caatatctat
     9721 cccgtccagg tctctgttac tcaactcgta cataggcaag tacctccact taaatttaaa
     9781 aattttttta tctttcaatt tgtcaaattg taaattctgt caaactgaca aaaagcgcta
     9841 aaagccttcc aacactccac ttaccaggta tcattgtttt aagtttgaca actcttcagt
     9901 atgacaagtt caagggaaat ttctttaatt tatcccctca gtttctcata tcttacattc
     9961 tgtgaaactc actccattct gtaaacccct gatatacctt actttcaagc tattacttct
    10021 tttcagttta tgctttcttc attttgtgaa actaatttac tgaaattaaa aacaaggcca
    10081 tttttgatat agctatcaat ttcttgatat tcaaatcagc cttgtttctg atgaattact
    10141 ctttacctag atattcttta atctcatgat attccttaga aaaattcata tgcccgctga
    10201 tatcaggatt taagaacgga aggatacttg ttggatttac ttctgtccga tatactgcaa
    10261 gagaatgttt ctgagttatt tctctaacta ctcctttatg tatttcttct acatctttct
    10321 ttagtgattg aatttcatcg taggcatcta aaattattct aagtttcttc tggtaacgtt
    10381 tatatatttt cttttcttca gctcttcgct tagtctcttt aaagatatat tcaaatatcg
    10441 ctgcctttgc ctcccagaaa taagtatcat actctttctc taaaagatcg attgattcac
    10501 tcattttagt aatttgttct aaggaagtag cattattctc aaaaaaagaa tcaatattat
    10561 caaacgaaac cttcttctct ccagtaattg tttttctttt ttcttccaac tttgttcttg
    10621 cttttgcaat cttatctttt ttatcatcaa gattttctaa tattgataat acttctctaa
    10681 tatccatccc ctatctccta attccatttt ataaagaaag cgcaatccgt ttcaattttc
    10741 ttcacaacga aacgagtata aagcattaaa ctcattccat aaataacgga ttcacttacc
    10801 caacgtaagc tatttttatt tctttcaaat aaagtggcga aattgtacaa atccccaacg
    10861 aaagcaactt tatcgccttt tactcctagt acttcatcag atacaacaat aacatcatca
    10921 acgtataagt tttctgagaa tcgttccttc ttgttaattt ttaaaatata atttccatca
    10981 ctagatttct ctttatctaa aaacttaaat agtgactgac ttaatacaat agtattatgc
    11041 cgttcaggat ttaaatcgtt tattgtatct ttcaattcgt caaaattaga aacatttttt
    11101 tcaggtgctt cttttagaat ttttccaatt tcaatattac gagttttacg acaaagacgg
    11161 gtaattttgt tacttaagaa gtctgatata ttatattctc catcatcaac ttgttcagac
    11221 gataagggaa tacgtcctga aaatgtctta tgttcaaact tggttttaat ctgttttttt
    11281 ctaagttcta cacttttact atctctaaaa tcttctgatt caagttctga tagatgttca
    11341 tcgtaaaaac ctacagtttc atatgttcca ccagtacccg tatgctcaat cacattaaca
    11401 agatccacta attctttccc ttcttcagga acgtcataga tacttgttat atcttgtgat
    11461 aatattaaat ctgtttttga ttttccatca tcactactca ttcctctact tcttacatac
    11521 ttttctacca aactaaattt tttagccatt ttatactcct ttatcttatt ttttagatta
    11581 tctgcctatt tttttatttt ttgttcaaat ttctctttta ttttttcctc tactggtttt
    11641 atgtgaggaa ttgctttgct gcgtccccca tttcttaata catgtccatg ttctaataaa
    11701 tgagttaatc tatatgttgg atctgcatta tagattacaa aagaaccttt agaatttttc
    11761 ttaaagcgcc aatttttcgc atatttccca tatcttttag gacttgtttg tttcaattca
    11821 ttcactgctt catttgtaac ctcttcagca attaaatcta tctgttcttc aacttcttca
    11881 gaataagctt ccaaagtttt agcaatttca tttgctaaat cactcgttaa actcatttta
    11941 tcctccttta tctctttatt gctcctcgtt gtttataatt ttttctaaag ttcttagttc
    12001 ttagcttttc ttttatgact ctccgagcct ttagaatcat tttttctaga ttttggtttg
    12061 tcttgtttgt tagcatattt ctctagtatt tcttgtttcc gttgttctaa gctatcacct
    12121 tcttttttac actgagaaaa gattttctgt cttttatctg gatccataga aaacttattg
    12181 gctactacat accctaaaga agtatctcct gacataatac tcaccccctt tctattcaaa
    12241 caaaaaggga cataccacta gcactaaatg cttacggtat gtccctgagt tgttctcaat
    12301 agacttattt tttagtttct tttttgacta gatgagtaaa tttcccatct gagtagacca
    12361 aagttatttc tccaaatctt ggaatttttt ctatctctat tataccacat ttttcgtaga
    12421 caataaagcc tttttctgtt gaaaatttca tttcatccat atctattaac ctttctctcc
    12481 tctcactgtg ttaatcgtat atcgcttatc tttgattgta aaagccttga aagtgttccc
    12541 ttctaaacct ttcaaaattc tacttgagtt tctagcattg tataccgtcc gcagttcgct
    12601 actatctagg ttcgtgttga aaatcgtagt ttctcgattg ttgataatat caaacaagaa
    12661 atcctgttcc cagtcactct taggagttac cgtcccattt tttgccccta ggtcatcgat
    12721 gattagaaaa tctacatcaa ctagcttttt aactgcctca tactctgtta agtttgcatt
    12781 tcttccataa gcccaacctt cttttatctg cttgataatc tcggttaagc tgacaaataa
    12841 gacactctta ggatcgttct tctctctgaa actctcattg atttctttgg ccagggcaag
    12901 cgataaatga ctttttccta ttcctgtgct accgctgatt aaagtatttc ccgtcatacc
    12961 tgcaaggtat ttctgagctt gcccctttac aaactctaac atctgacctt cctctgtcgt
    13021 cttcacaaag aaattatcaa acgttgcccc cttcaactcg ttaggaatcg tactatcacg
    13081 cattaagaca tcataagttt tgaaatatgt ttgtctatca tcaaacctct gtaataggtc
    13141 ttgctctttt tgtttaatgt ctcccttcac acattccggg caaaatggtt gtatttttct
    13201 ttctgaactc cctactacag gtatagaaat ttcccagtaa tttacctggt gaatatcgca
    13261 aactttatcc gatatttttt tgttattgaa ctctttaaat tgttccttca tctttgcaac
    13321 tcctaaaatg gtagatctgg gaagttatct tcagacttcc cttttatggt tttaggcttt
    13381 tgattcaaat aaccgtcaaa cttagatccg aaaagtgttt caggtctcag atatttagaa
    13441 aattcaggac tatccttcca ttctgccgtt ttaatatcta tcacctgttt aaaatcttca
    13501 agtgtatagc cttctttgaa tcgtgctagt aaaagccttt ttgtcttatc aacaaactta
    13561 taccgcttgt tggctacttg attcagataa gcaataggaa cccaaagttc tttatgtttt
    13621 gttctctcta aatcttttat agctgtttct tcaagccaag taggaaaagt taagtcggga
    13681 tttcccgaca atatattatc taaatataaa ttattactct tactattaac tctattctct
    13741 atctctatct ctgttggaca tgagttggaa atagtctttt tattttggac attctccaat
    13801 tttggtaaat cttgactatt ttttctttgt tctcgcttgt attttgccca gtttgtttca
    13861 ctctcaacca tggcttttgc ttgtgataat gtagcatggc catcatcgtc tatctgaatc
    13921 agtccacatt ttgtaaaata tgctactgtc atatttatat catcttcaga aacatccagt
    13981 tttaaagcta attcctgtac caaactatca aaatagcctt catagtataa aatacagtca
    14041 tcttctaaac tttccaacat aagacggata taaatcactg tcatagtgta gccgccaggc
    14101 atatttttaa gtcgtttaat aaaaagatta tcaaaaaact tcttatcaac ttttaaccaa
    14161 aaatatattt tagtctttgc catcacacac ccccagaaac ttccagatgt cagtcactaa
    14221 atagtaaatc attcttgaac catcatcagg cggttgatag cgtcttaaac cctgactttc
    14281 ccacttattc agcgtgttgt cactaatttt taactcttct ttgagttcaa ctctatttat
    14341 aagcttggtc aatcttggcg gtactttctc atgcgcttca aggtattttt ccactacctc
    14401 cagaatgcca tgcgttaggt ctttctcgct ttctcggctt aggctaaaca tatccgccca
    14461 cctccttcaa ggtttctttg tagctttcta aatcgctatt catcaacacc gctaggcgct
    14521 tgctctcctc ctgtacttgg ttgtaaaaag ccttagcgcc atctagtaac tcgcctttat
    14581 tcgccggaat aaagtaccca ctaaaaacgc cacatctaac ccctacaata gggatgttgt
    14641 gcttaactac tagacgcttg ataatgtcac gtacagttct ttctgttagc ttggttgtca
    14701 ggctgatttc taaccctgtt atggagttct cagcccctac cttgattaat cttaatactc
    14761 gtttatcatt ctctgataga cccatccagt tcctcctctc ctttactttt attcaaaaag
    14821 gttatttgtc cgctcgctac cccttgcaaa atagcttttt gattttctat gattgtatta
    14881 agggttttta aaataacagc cctagtctct aaatccaact cttgttttgc cagcaaaccc
    14941 atagttacag ctaactttgt tgcattgtcc gtacacatag ctagaaaaat ctctctggta
    15001 aaataaggtt catctggtat ttttttcaaa atcaaggtat atcttcactg tgtaactcat
    15061 ttcatccctc cggaaactct tacccctgca agctggatat atcgcccata atcagggttt
    15121 aaatcctcgc taggttttct atcgtctgtt ggttttctcg ctcaatttgg gcgctttttt
    15181 tgcggtctcg gtggtttaaa taaagcagta agccaatcaa aatcacgatt aagacagcag
    15241 cctgtgtgtt acttatatct agttcattca tactatgtcc tctctatact tatgagtgtt
    15301 ttttgtatgt tctacaatta gactttctaa tgcttgagat actacattga cggtagctgt
    15361 cttttcagaa tcttctccta cactagtcat tcctaaaact atcaaaatgt ctttcaacct
    15421 tccgcaaagt tcttcataat cagttaaaat gtcaaatttt tcctttacca tattgttttc
    15481 ctgttccttt ttcctgcttt ttcctataca cgattaccac cactccaaac gctgggcatt
    15541 tgcccctagt tggcggacgc ttgtagcggt gtttcgtgag taattaccca tctttcagct
    15601 aaacaaggcc ttagaatcac cctgtcagca cttgatttca aaaccttttc taattgcttg
    15661 cctgctcttc ggtttttctt tatgtatttg ataaaatagc tgttttctga tataatcgga
    15721 gcatagaaaa aatttctata tccttaatct tgtcgcttgc tctcctcggt caaaatttga
    15781 gcaagtgatt tttttatttt ctttttgcat gattactacc tgactttggt ttataaagca
    15841 aatctttact ttcgataaga tccagaatcc agctgaatcc ctgctccact gtttcaagaa
    15901 atgcgcctag gtcttcattg tccaagttct cgtagttcat acaaagatat tcggctagtt
    15961 gtctgtcttt ctcaactagc tttttaaaat ccttgggata cttgggaatt tctaaaccct
    16021 tggcatttgt aactgtctta aattcatttt ccattttcta tattcctatg ctttaaaaat
    16081 taattcctta atttctgaat attccctatt caagttaatc atagctattg ccatatcttc
    16141 caaacgttgg tagtttgtca gttccgcgct tgttaaactg tcaataccgt tttcactttc
    16201 tcgctcttgc atgagttggg ctttgttttt cccagtcgct ccctttagca gtaggtttgt
    16261 aagtgtacta taggcatgct tgggcgcttt ctcccatgat ttgatagctt cggttaaggt
    16321 cttgcgcttt ggcttttcca gttctctttg aagatagcgt ttagaaagtt catcacgcat
    16381 ttcaaaaaag gctttaacca ggttcttctt aaactctttt acgggttctg tatttcgtaa
    16441 gtaagtgatc atcaaggttg cttgttgctc attcaaaata tagtcccgta cattttgccc
    16501 actctttgaa ggtgaaattt taaattgcac ctttccgaag ctttcaaagt cctctcggtg
    16561 cttgttcagc aaaatcttca aatgtctgtg cttaatttca gcgcagtcgg caacgatact
    16621 gctcagtgta tacggctctt tcttgccgtc catataaacc aattccattg gttcgctcct
    16681 ttcttcttgt tgctcgtgct tgccacctaa aacagtacca aggtaaagca ttaaagtagg
    16741 gtaaaatcgg agaatatgaa cccctacaaa cccttgatac tgctataggt agcaagcaag
    16801 cgaatattta gctagatccg tttatcaatc cccagtagta aagcaccaca tgagaaatct
    16861 ataaatgtag agtagtatta cgattggttc gctcctttct attctttcgt atctagaaat
    16921 tcatccagcg taacgcccaa gtaactagat acttttttta acgtatcaag acttggattc
    16981 tttgcacgtt catagtacaa agccgttaaa gtgctttttg aaattcctgt agcctctgca
    17041 acatcagcag ttttcttacg ttgcttggct aataacaatc tcatgttatt cttcatagca
    17101 taaaacctcc ttttgtttaa aaatttcacc acaaaagatt gaaagatatt ttatatggtg
    17161 tactttttgt gcaactatat gttgacaaaa atat
//
LOCUS       DAVKGC010000035        16461 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKGC010000035 DAVKGC010000000
VERSION     DAVKGC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.035"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..288
                     /locus_tag="VW049_000964"
     CDS             <1..288
                     /locus_tag="VW049_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9432109.1"
                     /translation="QNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLH
                     YSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(388..2010)
                     /gene="groL"
                     /locus_tag="VW049_000965"
     CDS             complement(388..2010)
                     /gene="groL"
                     /locus_tag="VW049_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9432110.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2026..2310)
                     /gene="groES"
                     /locus_tag="VW049_000966"
     CDS             complement(2026..2310)
                     /gene="groES"
                     /locus_tag="VW049_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9432111.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2580..2975)
                     /locus_tag="VW049_000967"
     CDS             complement(2580..2975)
                     /locus_tag="VW049_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9432112.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3053..3814)
                     /locus_tag="VW049_000968"
     CDS             complement(3053..3814)
                     /locus_tag="VW049_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9432113.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEALKA
                     EFAETFPNQTVWTFLLDVTDMTIVKTVCSDILETIGQIDILVNNAGLALGLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKANQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(3847..4473)
                     /locus_tag="VW049_000969"
     CDS             complement(3847..4473)
                     /locus_tag="VW049_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9432114.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4489..4806)
                     /locus_tag="VW049_000970"
     CDS             complement(4489..4806)
                     /locus_tag="VW049_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9432115.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(4803..5102)
                     /locus_tag="VW049_000971"
     CDS             complement(4803..5102)
                     /locus_tag="VW049_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9432116.1"
                     /translation="MNGMKAKKMWMAGLAMLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATFYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5300..5488)
                     /locus_tag="VW049_000972"
     CDS             complement(5300..5488)
                     /locus_tag="VW049_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEU9432117.1"
                     /translation="MKASTWVYDKGEWYYVSSSGAMLANDWVKDNGKWYYLASSGKML
                     RNIYTPDGYYVGNSGAWQ"
     gene            complement(5739..6413)
                     /locus_tag="VW049_000973"
     CDS             complement(5739..6413)
                     /locus_tag="VW049_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432118.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(6419..6865)
                     /locus_tag="VW049_000974"
     CDS             complement(6419..6865)
                     /locus_tag="VW049_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9432119.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(6979..7482)
                     /locus_tag="VW049_000975"
     CDS             complement(6979..7482)
                     /locus_tag="VW049_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9432120.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7479..7841)
                     /locus_tag="VW049_000976"
     CDS             complement(7479..7841)
                     /locus_tag="VW049_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU9432121.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7907..9772)
                     /locus_tag="VW049_000977"
                     /pseudo
     CDS             complement(7907..9772)
                     /locus_tag="VW049_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(9804..10253)
                     /locus_tag="VW049_000978"
     CDS             complement(9804..10253)
                     /locus_tag="VW049_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9432122.1"
                     /translation="MDKPMLVFKRFGHQIYLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(10377..11093)
                     /locus_tag="VW049_000979"
     CDS             complement(10377..11093)
                     /locus_tag="VW049_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9432123.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11974..13389)
                     /gene="ply"
                     /locus_tag="VW049_000980"
     CDS             complement(11974..13389)
                     /gene="ply"
                     /locus_tag="VW049_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9432124.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13400..13732)
                     /locus_tag="VW049_000981"
     CDS             complement(13400..13732)
                     /locus_tag="VW049_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432125.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(13803..14411)
                     /locus_tag="VW049_000982"
     CDS             complement(13803..14411)
                     /locus_tag="VW049_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432126.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14424..14882)
                     /locus_tag="VW049_000983"
     CDS             complement(14424..14882)
                     /locus_tag="VW049_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9432127.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15007..15406
                     /locus_tag="VW049_000984"
                     /pseudo
     CDS             15007..15406
                     /locus_tag="VW049_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16170..16430)
                     /locus_tag="VW049_000985"
     CDS             complement(16170..16430)
                     /locus_tag="VW049_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432128.1"
                     /translation="MRERSATGAQDLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT         4851 a         3753 c         2969 g         4888 t
ORIGIN      
        1 cagaataagg aaccagagaa atttttcgga cttattgagg acaatcttaa gcaggttcat
       61 cctatttttc agactgtctt taaaaccttc ctcaaagata aagaaaagat tgtcaacgcc
      121 cttcaactac actattctaa tgccaaactg gaagcgacca ataatctcat caaacttatc
      181 aagcgcaatg cctttggttt tcgaaacttt gaaaacttca aaaaacggat tttcatcgct
      241 ctgaatatca aaaaagaaag gacaaaattt gtcctttctc gagcttagct tttcttcaac
      301 ccactacagt tgacaaagag cctataaaaa gaagggttag tcattccctc cttttgtgtt
      361 ttttataagt tgttttctat agaaagctta catcatcccg cccatcatac ttggatccat
      421 tgctggagct ggggctactg gttctggttt attggctacg actgcttctg ttgtcaaaat
      481 caagctggct acagatgctg cattttgtag ggctgaacga ctcactttaa ctggatcaat
      541 gataccttga tcaatcatgt taacccactc gccagttgct gcgttgaatc ctataccaag
      601 ctcagcattt ttcaaacgat cgataacgat agatccttca aatcctgcat tgtgagcaat
      661 ttgacgaaca ggttcttcca aagcacggag aacaatatta cgtcctgttg cttcatctcc
      721 tgtcaattcc aaggtagcaa cagctggaat cacattggca agagctgttc caccacctgc
      781 aacaatacct tcttcaacag ctgcacgagt agcgttgagg gcatcttcaa tgcggagttt
      841 catttctttc aactcagttt cagttgcggc tccgacctta ataaccgcta caccacctga
      901 caatttggcc aagcgttctt gcaatttttc acggtcaaat tcagaagttg tagtttcgat
      961 ttgagacttg ataaccgcaa cacggtgaga aatcgcttca ggatttcctg caccttctac
     1021 aataaccgtg ctatctttgt ccacggtcac tctcgctgct tgaccaagag cttcaattgt
     1081 cgcatctttc aactcaagac caaggtcttc tgtgataact gttccgcctg ttaagatggc
     1141 gatatcttca agcatggctt tgcgacggtc accaaaacca ggtgccttga ctgctactac
     1201 gttgaaggtt ccacgaatct tgttcaaaac aagagttgga agagcctcgc catccacatc
     1261 atccgcaata atcaagagtg gacgattgct ttggagaatg ctttccaaaa gtggcaagat
     1321 ttcttggata ttggaaattt tcttgtctgt aatcaaaatg tacggatttt caaggtcagc
     1381 caccattttt tcgctatcag tcaccatgta ctgtgaaagg taaccacggt caaactgcat
     1441 tccttctacg acttcaagct ctgtttccat accacgtgac tcttcgatgg tgatgacacc
     1501 gtctttgcca actttttcca ttgcttcaga gatgtactca ccaacttttt cagaacgaga
     1561 agatacggct gcaacttgag cgatagcttc tttattggca acagggatgg cgttgttttt
     1621 caaagcttca actgctgcgg caactgctgt ttcaatccca cgacgaatac cgattggatt
     1681 tgcacctgct gtgacgtttt tgattccttc acggacgata gcttgggtca agactgtcgc
     1741 agtcgtagtc ccgtcacctg cgatatcatt ggttttagaa gctacttctg atactaactt
     1801 agcacccata ttttcaaaat ggtcttccaa ttcgatttct ttggcaatgg tcacaccgtc
     1861 attggtaatc aagggtgaac cgaatgactt ttcaagaacg acattgcgac cttttggtcc
     1921 caaggttact ttaacagtgt ctgcaaggat atcgacacca cgaaccatgg ctgaacgggc
     1981 atctgatgaa aatttaattt cttttgacat acttactttc tccttctatt cctcaatgat
     2041 tgccaagatg ttagcttcgc ctacgatgat gtacttttca tcgccatctt tgacatcaag
     2101 acctgcgtgg gcttcaacta agacacgatc tccagtttta acacttggag caaccaagtc
     2161 accgttcaag gtacgaacac cttgtccagt agccacaact tgagctgttt tggttttttc
     2221 ttgggctgag cctgcaagga caaagccccc aacggtttgt tctttttctt ctatttttaa
     2281 gaccacacgg tcccctaatg gtttcaacat ctgatttcct ccataatgag atagatatta
     2341 ttagcactct ttgaacttga gtgctaattc ataattctat tgtatcactt ggtcagaaat
     2401 agtcaagaaa aaagtctgac tttctcaaga taaaaagcct gagaccaact cagacttttt
     2461 aatgtttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggctgctcaa
     2521 agcattgctt tgatgttgtg gataaagcta acgtggtttg aagagatttt cgaagagtat
     2581 taaaatggca attcttcctc ttccaagacc aaatctgcca aatcttggcc tgcattattt
     2641 tcacgcatgg cacgttgagc acgactttct aagagttgga atcctgtcac aaggacttcg
     2701 gttacatagt tcatctgacc atttttctca aagcgacggg tacgcaattc tccatcaacg
     2761 gaaatgagac tacctttggt tgcgtagctt gccaaagttt ctgctagcct gccccatagg
     2821 accatattga caaaatcagc ttcacgttca ccgttttggt ctttgtaacg acggttgaca
     2881 gcgatagttg ctcgcgctac cgacttgtca ttgttggttt tgtgcaattc tggtgtagac
     2941 gttaaacgcc caatcatgat aactttatta tacatatttt cttcctccta cttatctatt
     3001 cgtaggaaat caaaaaaagt tacagaaatt tgtaactttt cgaggaaatt tcttattttt
     3061 tatgaatcat gaaacctgtc gcctgttgat tggccataat ggtcatatct gtaatctgaa
     3121 cacggcgagg ctgactggtc acatagacta ctgtgtctgc aatatcctga gcttgcaagg
     3181 cttctattcc ttggtaaacg gacgcagctc gttctttatc accatgaaaa cgcactgtag
     3241 aaaaatctgt ttcgacaatt ccaggctgaa tggtcgtcac cttgatatcc gttgcgatgg
     3301 tatcaattcg cagtccatct gaaaaggtct taactgccgc cttggtggct gagtaaacag
     3361 ctgcaccagc ataggcataa attcctgcgg ttgaccccat attgataata tgaccttgat
     3421 tggcttttac cattgctggc aagaaacagc gagtgactgc catcaaacct ttgacattgg
     3481 tatccaacat ggtcagcata tccaactctt catagtcttg atagggagct aagccaagag
     3541 ccagtcctgc gttattgacc aggatgtcaa tctgacctat cgtttctaaa atatcagagc
     3601 agacagtctt tactattgtc atatccgtga catctaggag aaaagtccaa actgtttgat
     3661 ttggaaaagt ttctgcaaac tccgccttaa gagcttctag tctgtctatc cgtcgtcctg
     3721 ttagaacgac atcctcaccc tgctccagat aagcacgcgc aatcgcttca ccgattcctg
     3781 aggttgctcc cgtaatcact acattttttg ccatcttatt tccttctatc agatgctgac
     3841 aagttcctag gcagtccagt gtttcgctgg gtcgaacggt gttccgacaa cttggtcttc
     3901 tgataattca agcaccccac gtttttgcgg agcatttggc aaatgcaatt cacgaggact
     3961 acacatcatc ccaaaactct tttcaccacg aagttcgcct gggaaaatga aattcccttt
     4021 tggcatcatg gcaccaggaa gagccacaat ggttttcaac ccaacacgcg cattaggtgc
     4081 tcctgcaacg atttgcacta tcttgtcact tgcgactgca acttggcaga tgttgaggtg
     4141 gtcactgtct ggatgagcta ccatctcgac aatttcacca acaacaaact taggttcctt
     4201 atcattaaca atttcttctg taaaaccttc cgcctgtaat tcttggttca aacgagcgac
     4261 ttgctcatct gataaaaaga cttgaccgcg ctctgcaatt tcaaacaagc tcgaaacctc
     4321 aaaaatgttc caagctactg tttccccatt ttctttgagg aaaacacggg ctaccttgcc
     4381 tttgcgttcc acattcagtt tggcatctcc gctatttttc acgatgacca taaggacatc
     4441 accgacatgt tctttgttat acgtaaaaat cattgtttcc tttttctcct atttcaatcc
     4501 tgctaaaaaa tcgttaattt gctccttact tttacggtcg cgattgacaa aacgaccgat
     4561 ttccttgtcc ttttctagaa caacaaggct aggaattccg taaacatccc agagtttggc
     4621 caaatccata tactgatctc ggtccattcg aataaaggtg aactctggat tggtctcctc
     4681 aatctctggt aaggcaggat aaatataacg acaatcgcca caccagtctg ccacaaaaag
     4741 gaagaccttc ttgcccgctt tttccactaa agatgctaat tcttctaaac ttgctggctg
     4801 tatcataaga cttcctcctc atagactagg tcttcatttt catagacaaa ggtataatga
     4861 cggccatcct caaaaatgac gccaccaacc aagctctcca gactgctttc gtaaacttga
     4921 acatagaagg tcgcaatttc ccccatgtcg gaaaaatggt ctcgcacaat ctctgtcaac
     4981 tcttcctgag tcttcatgag cttacggtca tctgcaactt ttttcgtggc aagagcaagg
     5041 cttccgatac ctagcatagc caagcctgcc atccacattt ttttagcttt cataccattc
     5101 attttaacac aaaaaaggct tcaggacaaa tgaggaagca gcagaaaagc aagtaaaaag
     5161 cctcttcctt taaggaaaag gacttcttat actaagagca aactaggaag ctagccgcag
     5221 gctgctcaaa acacggtttt gaggttgtgg ataaggcgac gctgacgtgg tttgaagaga
     5281 ttttcaaaga gtattattct tattgccagg cgcctgagtt gccaacgtag taaccatcag
     5341 gtgtgtagat attgcgaagc atcttacctg atgaagctag ataataccac ttgccattgt
     5401 ctttgaccca atcattcgct agcatggctc cagaagaact tacataatac cattctccct
     5461 tgtcataaac ccaagtgctt gctttcatgg ctcctgagca attaaaggtt caaaaaactg
     5521 tccaatatca ttcatttttt taaagcattt tacactacat catccacgct gtaggacata
     5581 gatacatcaa atgtaagagc caaaatatcg aaaaatatac aacttttaaa ggcaacattt
     5641 cattttttaa agtctaacaa ccatagaact gaaatcagag caaggcatca acttcctact
     5701 cttagtacgt ctattttgat ggttgaagtg aagatttttt atttaacgta gacaatttcc
     5761 aacttcccaa aagccacatc tccacggata atcaaggttt tgctggttgg ggctacagga
     5821 gcgtcagcct tagctgcacc aaaggaggtt tctactttca aatctacacg ccagtgttgt
     5881 ggaacataga cggttgcatt tccgaaggcc acttcaatat ttaaagttgc aaaatcacct
     5941 agcatctctg cattatcata gtagatttta gcatccccaa aagcgacttc cacttggtca
     6001 tctacgagat cttgatcttg cttatagaag gtcccgctac caaaagcgac ttccttatcc
     6061 gtgacgactg tttttttccc attgtaccac catttttttt cattccagaa cttacttgaa
     6121 tgcgtcagat aaccaacacc aagtaccacc aagatgctag cccaaataag agaatgattg
     6181 gtaactggta acaagtcata agcgtaattt gcaatgatga gcgccagtaa acctgtaaaa
     6241 actgccgaag tgagatgacg tctaaggatg gactcaatgg acttataagc aaaaaaaaca
     6301 atacctagta aaggccatat tttaccatcc aaagaaggaa ttccaaaatt cccttgcagc
     6361 aagatccaag ctgctaaaac caataaaaca ataccaaatg ctttcttttt catgtttttt
     6421 acctcatgtt tcttagattt tcttttagga gggattggta atgccgtgag acatgaacct
     6481 ccttgtgcgt ctgataaaag gaaatggtgc ccgttcctga aaaggacttg tccactgagt
     6541 aaatctgacg gatgtttgcg atcgttgact tggaaactcg attaaaatag cgaggcaaga
     6601 tagactccaa ctcatagagc ttgaggcgaa cctcataagc ttccttctgg ttatgagcgt
     6661 agatcttgct cccttctgtt tcaaagaaga gaatttctga caggtctaga taatattcac
     6721 ctgtcccctt gtaaaaagtc aacctcggag cctttgactc ttgcaagagc cgttgcaagt
     6781 ctgcaatttc atctgtcaaa gtgggggtct tgatgacaat ttcagtctcc tctaaattgc
     6841 cgtcaatctc aattcgtaac ttcatcacat ctcctttctg actctactat atcaaagcta
     6901 agttaggaat acaagggcaa aaaagcaagt ggtcaatttg aagcagtaag tggttgtagg
     6961 actagcttaa cttacatgtc agaataggaa gaaaaggaat ccccacacca aaccacaagc
     7021 atagccaacc aagacatcct tgggataatg aacaccccct aaaaccctca ccaaccccag
     7081 caaagctgaa aagatcaagc atactaagcc aataggtaga ctagcatgca gacaggccat
     7141 ggagatgata gttgccgaaa agacatggcg actgggcatc gaccgtcccg aactatcctt
     7201 atcaagaagg ggacagatat cccaagtttc ataaggtctc gggtgattga ttttcttacg
     7261 aaacagggac aaggccacaa aacctgatgc aggaataagc acataaaccc caacttgctg
     7321 accaagtcct agttgcaggt aagtggtgac tagcaagatc agatagacca ggggcatggc
     7381 gactgtcatc aaacgattga aactgcgtaa taggaataaa agaattggac gactagttag
     7441 tctggagctg atatttcgat accattcttg ataatttttc atttatgctt ctcatacttt
     7501 tctagaactt gttgagatgt tttccccttg gcaagccaat ccactagctt gtccaagcga
     7561 cgtatttcct gcatcagagg atcatcaatt tcctcaatac ggacaccgca aatcttccct
     7621 gtaattgcaa ttcgttcagg gacataatat ggtgcttgtc taaaaaaatc tccgtaagta
     7681 acatccctat ccagacaagc aagaacatca ctcacttcat accctgtcaa ccaactggtc
     7741 acttggtgaa ctgactctgc ctttccaccc tttcgctcaa cttttgcaat caaggctaag
     7801 tagacagcag caaatttcat attgtataat ttctgactca tacttctctc tttctatgtg
     7861 tactagtata aataagaaaa agaaggccgt caagccttct tttgatttat tcttctgctt
     7921 catcttctgt aaattgacta ttgtacaagt cagcgtagaa gccaccttgc gccatcagtt
     7981 cctcatagtt gccttgctcg atgatatttc catctttcat gaccaagatc aagtctgcat
     8041 ttcggatggt tgacaagcgg tgggcaatga caaaggatgt gcgtcattcc atcaaacggt
     8101 ccatggcttt ttggatcaat tcctctgtcc gtgtgtcaac agaagaagtc gcctcatcca
     8161 aaatcaaaag cggtgcatcc ttaagaaggg cacgagcaat agtcaatagt tgtttttgtc
     8221 ctacagacaa ggtcacggtg tcatccaaga tggtatcata gccatctggc aaggtcataa
     8281 taaagtggtg aattcccaca gccttactag cttccatcat tcgttcatca ctaatcccta
     8341 tttgattata gatgagattg tctcgaatag ttccttcaaa gagccaggta tcctgcaaga
     8401 ccattgaaaa ggcatcatgc acttctgaac gcgtcatagc cttggtatcc acaccatcaa
     8461 tgcgaatact tcccttatca atctcataga atttcatcaa aagattgaca atggttgtct
     8521 taccagcccc agtcggccca acaatggcaa ccttttgacc agcatgagct gtcgcagaga
     8581 agtcatagtc ttgaacattg acaccgtcca ccagaatttc tcctgctgac acgtcgtaga
     8641 aacgtggaat cagattgacc agagttgatt taccagaacc tgttgaccca ataaaggcca
     8701 ctgtttgacc agtttctgct ttaaagctaa catgttcaat aactgcctcc gaatttgccg
     8761 catagcggaa ggtcacatcc ttaaactcga cctgaccttt gaagttttca tcagtcagct
     8821 gcacttgaac agggttttgg atagaagaat gcaaatctaa aacttgatta atccgcttag
     8881 cagagaccat agttcgggga agaacgatga agagtgctcc catgagaagg aagcccatga
     8941 caacctacat ggcataagac atgaaaacaa tcatgtcact aaagagaggc agacgcgcta
     9001 tcggagcagc gtcgttaatc acataggccc caatccagta aatcgctaca ctcaaaccac
     9061 ttgaaatccc catcatgata ggattcaaaa tagccataag acggttgaca aacaaattca
     9121 aacgggtcaa ttcatcattt actgctgcaa atttttcatt ttgataatcc tctgcattgt
     9181 aggcacgaac gacacgaata cctgttaaac tctcacgagt gatactgttc agtttatcta
     9241 tcagcccctg aatcaaggac tgttttggaa agtctagcgt catcaaaacg gtcgtcatca
     9301 ggacgttgac aatcactgcc acaagtacgg cccaaagcca gtattctgaa tgacctaaaa
     9361 tcttcccaat agcccaaata gccataattg gaccacgcgt taccacttgc aagcccatag
     9421 taatcaacat ttgaacttga gtaatgtcat tggtagtacg cgtcaagagg ctaggaattg
     9481 aaaatttctt aatctctgtc tgcgagtaat ccaaaactcg gttaaaaata tcacttctca
     9541 gcctactagt ataagaagcc gccactcggg atgcaaaaaa tccaactgca actacggaca
     9601 agaaggcaag aaagagcatt cccatcatca tgcttgccgg ctgccacaac tcatctaaat
     9661 tagttccttg actacctagc aaatccgtaa ttttcgagat ataggtcggc acttccaact
     9721 ctagatagac cgaaaagcaa gtaaagagaa tggctagtaa aatcatcccc attcttttct
     9781 actaattctt ttggctaatt tctttattct ctcctcctat tcccttgata ttttgcctgt
     9841 agttgaccga gaactttctc aaaaatcagt aattcatctt catcaatgtc ttccatcaac
     9901 tgcttgtcta tgcgttcaaa aaaagcctta acctgttgca tctgagaacg tgctttgtcc
     9961 gtcagacgaa caaacttagc ccgcttatca acaggactcg cctccaattc caccaaacca
    10021 ttttgcacta tacgcttaac cagattacta gcaacagact tggtaatatt gagttcctgc
    10081 tcgatatctt taatcaagac caagtcttgg tttttctcgc gattatccaa aaaacgcaca
    10141 acctgacctt gcggcccacc cataaattca atgccgcaac gtttggcttc cttttgcacc
    10201 atcaggtaaa tttgatgacc aaaacgctta aagactaaca tcggtttatc cataatctcc
    10261 cccttctaaa taaaaatagt tctctggaga ataattaaat ttctatgaga actattttct
    10321 tgattaaaaa aatcccaagt gattttctca cttaggatca tgttctatag gttaaattaa
    10381 aacccatcta cgttcgtata aatcttttgg acgtcttcgt cgtcttcaag aacgctgtaa
    10441 agtttttcaa aggtttcaag gtcttcgcct gacaattcca cttctgactg aggaatcatt
    10501 tccaattcag tcacttggaa ttcttcaata ccagactcac ggagagcaac gatagccttg
    10561 tgaaggtcag ttggcgctgt gtaaactgtg attgtacctt cttgtgcttc tacgtcatcc
    10621 acatccacat ccgcttcgag caattgctca aagactgcgt ccgcatcttc acctccaaat
    10681 acaataacac ctttgttgtc aaaaaggtaa gaaacagaac ctgaagcgcc catgtttccg
    10741 ccgtttttac caaaggctgc acggacattg gctgctgtac ggttaacgtt agaagtcaaa
    10801 gtatccacaa ttagcataga gccatttggc ccaaaacctt cgtaacgtcc ttctgtaaag
    10861 gtttcgtctg tgtttccttt ggctttatca atcgctttat cgataatgtg ttttggcact
    10921 tgggcttgtt tagcacggtc gataacgaat ttcaaagctg agtttgattc tggatctgga
    10981 tcaccttttt tagctgctac atagatttct acaccaaatt ttgcatatac tttagagtta
    11041 gctccatctt tagccgtttt cttggctacg atattggccc atttacgtcc cattaggaat
    11101 ctcctttttt cacattttaa tctttcttat tataacacaa gtttttttga ttttcactag
    11161 aggaaatgga ttttattagc aaatcaagct aggatagtac tttacctgct aagatggtct
    11221 tgcctttcta tctttatcaa cagacactca tccacattca aaaaacaaac tagaccatta
    11281 tctgcaaata gaaagtttca gccaagtttg acaaagtcag ctcaaattac tgtttgaagt
    11341 ttgtagatat aagcgacaaa aacaatcata ctgcaccttt tgttgacagt ctactccaga
    11401 catatcatag ttccagtaaa tactttgaaa tccaacagtt cttataggcg ctattgtatt
    11461 ctaagaaatc aatagaagag tttctaagca aacctctaat actcaataaa aatcaaagag
    11521 caaactagaa agctagcctc aggttgctca aaacactgtt ttgaggttgc ggatagggct
    11581 gacatggttt gaagagattt tcaaagagta taatttacgt gttcccaaga tggagaagtt
    11641 agactagtac actggcactt ctaaaccatt gctagcaatt gatttgttca tatttaattt
    11701 cattttttcc ataaatgggt attagatata aacagcaaaa tatttccgat acgtgtcgtt
    11761 cttgaatttc caatcatcta aaacaagtaa aggataatca atcccctgta tatcaaggaa
    11821 ttggctaccc tttttacttt tttacacatt ctgtttgata gattcatttt aacatcacga
    11881 gcatactcca atggaaatcg ctaggcaaga gataaacttt cagatatccg cagagagatc
    11941 atcgcctctt tttgtcgcaa gcattctcct ctcctagtca ttttctacct tatcttctac
    12001 ctgaggatag agagttgttc cccaaataga aatcgtccgc ttacgcacta gtggcaaatc
    12061 ggttttttca taaaccgtac gccaccattc ccaggcaagc ccggtacact ctctaatttt
    12121 gacagagaga ttacgaacat tcccttttaa aggaatacta gtggtaaagt gagccgttaa
    12181 atcctgccca tttctgtccc aagccttagg agtcaagact tccttacctt gatgatcata
    12241 ggataattca ttccaagtaa tataatattg ggcaacatag gcaccactat gatccagcag
    12301 taaatctccg tttctgtaag ctgtaacctt agtctcaaca tagtctgtac tattttgaaa
    12361 ggtcgcaact acattgtcac gtaaaaaaga agttgtatag gaaatcggca agcctggatg
    12421 atctgctgta aagcgactgc cttcttgaat caagtcctct accatatcca ccttgcctgt
    12481 tacaactcgg gcacccgaac ttgggtcgcc ccctaaaata accgccttca cttctgtatt
    12541 gtccaaaatc tgcttccact ctgtctgagg agctaccttg actcctttta tcaaagcttc
    12601 aaaagcagcc tctacttcat cactcttact cgtggtttcc aacttgagat agacttggcg
    12661 cccataagca acactcgaaa tatagaccaa aggacgctct gcagaaattc ctctctgttt
    12721 taaatcctct accgttacag tatcttgaaa cacatctcct ggatttttaa cagcgtctac
    12781 gctgactgta taataaatct gcttaaaatt aacaatctga atctgctttt cgcctgaatg
    12841 gacagagtta aaatcaatat caagagaatt ccctgtcttt tcaaagtcag aaccaaactt
    12901 gaccttgagt tgttccatgc tgtgagccgt gattttttca tactgcattc tagctgggac
    12961 attattgacc tgaccataat cttgatgcca cttagccaac aaatcgttta ccgctccgcg
    13021 aacacttgaa ttgctggggt cttccacttg gagaaagcta tcgctacttg ccaaaccagg
    13081 caaatcaata ctataagtca tcggagcacg atcgaccgca agaagagtgg gattattctc
    13141 taacaaggtc tcatccacta cgagaagtgc tccaggatag aggcgactgt cgttggtagc
    13201 tgttacagaa atatcacttg tatttgtcga caagctccgc ttctttcttt cgataacaac
    13261 aaactcatcg ggtagctgat taccctcttt gatgaaacga ttttcaatac tttctccctg
    13321 atgggtcaag agtttctttt tatcgtaatt catagctagt ataaagtcat ttactgcttt
    13381 atttgccatc ttctacctcc taataagttc ctggattgag ttgcataaac tcagacttgt
    13441 tcagcgaaat cagccgtggt tggactaagt gatccaaaat ttcctcgtac aattcttctg
    13501 agacattgcg tcgccgtctg gctagataag aagtcggaat aaccgtatta tccaacataa
    13561 ataccttatc taagtcaatc aaggttggtc ttgtaaaagg attacgagct agatccggct
    13621 cttctatcat aaagttcttg accaaacgtc tggtcaagag agctggtttg aaggtctgat
    13681 ttttaaccaa ctctttgttt ttagtcatgc tgttgtcaat acagatatac atatgattct
    13741 tcacagccaa atcgctacta atagtcggaa aaggcaaata aagagctaca acatctcctc
    13801 tcttaatcaa gcaagagcac ccccttttct cctaatgtaa catagacagg attgaccaag
    13861 tcttctgatt gactcagaat ttccaaagtt tgagtttggc gcgctgtcaa tttagtagca
    13921 tcttgtctct tcaatacaaa atgcttgtcg ccaataacct tgacaatata atccttctcc
    13981 aaagctgact ggtaaatcca catcagatgt tgtctgtcct gagaactcaa gagagaagga
    14041 ttttcaagcc tcccgatagt ctgataaaaa tcaaaaacag gagctaactc ctgccaatct
    14101 gattggctag ttgtcaaggc tagaaaaagg gctttgcgag ctgatacttc ttggttagcc
    14161 ttgagagtta ctttcccctc caagtttttt agaaatcggg aaactccaga aagcaaattt
    14221 ttctctaact gcgagaaata aaaacctttc gttcccagac ataagtcttt catgtcgctt
    14281 tctctagcaa ataagagctc aaacatttga tagtaaaaga aaaatatctg gcactgggtc
    14341 gcgctcatct tttccttatc ggcttctttt tttaaccaga gcaagggcga caggtagctg
    14401 gattgagaca tttcctctac ctcctactct tttttaactg gagcatctgc actagctgcc
    14461 acttcttttg actggatact ttcccactgg ttaatctcct ctgagataag accttcgcat
    14521 gtcttgacaa atagggcaaa agccttggtc tttcctgcat atttctccgt ttggcattga
    14581 tagaggaatt tttctttctc caggagttgc gcagtttttt ggtaagaaat ccaattttcc
    14641 tttgcattat acaaattgat aatcccctca cacagcaagc cgagactgga taaggcaacc
    14701 gaaatcaaac ggtagcgatc acctggcata ggaatagcac aaaagacagc tatgaggaaa
    14761 cctgccacga tttctgttat ttttaatacc ttatagcgcc tacgatgttg aacgcttttc
    14821 tttaaaaaat gagctatctg tacgtctaat cgctctgtca ggtacatttc ttctggcgtc
    14881 atattcgtaa ctcctttcat ttactttgat aaccatagta taacatattt gaatatcaaa
    14941 gtatagtaaa aactggacta taacttatta gacttcctgc gaaacaaaat atggtatagt
    15001 agttctatga attatgaagc aagtaaacaa ctaactgata cacgatttaa gcgtcttgtt
    15061 ggtgttcagc gtaccacttt tgaagagatg ttagctgtat taaaaacagc ttatcaaaaa
    15121 agtcggacaa gctggtaaaa tcttggctga cagtaattat caagggctca tgaagatata
    15181 tcctcaagca caaactccac gtaaatccag caaactcaag ccgctaatag ctgaagataa
    15241 agcctataac cgtgcgctat ctaaggagag aagcaagatt gagaacatct ttgccaaagt
    15301 aaaaacgttt aaaatatttt caacaaccta tcgaaatcat cgtaaacgct tcggattacg
    15361 aatgaatttg agtgctggta ttatcaatca tgaactagga ttctagtttt gcaggaagtc
    15421 tattatatat atgcacagac atatgaaatc tttttgatac agccttcttt ttacatagag
    15481 agacagcagt tatgatatat ttatccatac acaacatctt ctgtaccaat actacaatat
    15541 tggtacattt tgattacaca atcgtttcaa aaaatatgat ggaagcctga gtattttata
    15601 gtaattccct tcctatcaac tagatcaacc agttgagtgt tgaatgttga aacaccctag
    15661 cctcagcaaa ggactatgat cagcatttcc tagccaatag gacgctccaa atcacctatt
    15721 ctaagtccat aactagcttg gtcagtaaga tacagactgc aaggcgagac tgacgtggtt
    15781 gatgagattt ttgaagagta ttacaatcat agattcacca gtagacttga acgcagattt
    15841 aactatagta gattgaaact agaatagtac acctctgctt ctaaaacatt gttagaaatc
    15901 gatttgactg tcctgatcaa tttgtcctgt tctcgtttca ttttactata ctaaaaaacc
    15961 acctgaaaca aatcaagtga ctagaggata aaaacaaaga ctgggcaaaa agttttgaaa
    16021 agcaaaaaac gcataatatc aggtcttgaa aaaccttgat actatacgtt tgattgtggg
    16081 aagattgact tcattttctc ctgaaattga agttttttct agtttccaga tatagacgaa
    16141 ttcaatatag ccatctcgct acaattattt taaccatcga gaaaattttt cagaatttca
    16201 aaaatttcat tttgatccaa tgaaatgtca tcattttgaa gaatagttga cttcacaggc
    16261 tctaattcta tcacaaagcg gtgcatgtct gcttttaccg cactacttga tgttatagcc
    16321 gataactttt catctcctag caaggaagct gtcaaacggg taaggtcatt caaatgcttt
    16381 ttaatggact tacttaaatc ttgagcacct gtagcagagc gctctctcat atccagccaa
    16441 gctttagcct taaaaataat t
//
LOCUS       DAVKGC010000036        16098 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKGC010000036 DAVKGC010000000
VERSION     DAVKGC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16098
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.036"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            33..254
                     /locus_tag="VW049_000986"
     CDS             33..254
                     /locus_tag="VW049_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9432129.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            771..1024
                     /locus_tag="VW049_000987"
                     /pseudo
     CDS             771..1024
                     /locus_tag="VW049_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1152..2381
                     /locus_tag="VW049_000988"
     CDS             1152..2381
                     /locus_tag="VW049_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9432130.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2401..5097
                     /gene="ppc"
                     /locus_tag="VW049_000989"
     CDS             2401..5097
                     /gene="ppc"
                     /locus_tag="VW049_000989"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9432131.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDCIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5180..5397
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5510..6514
                     /locus_tag="VW049_000990"
     CDS             5510..6514
                     /locus_tag="VW049_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9432132.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATSAAQGLASATKDLPVIMAAITDPIGANLVKGLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6583..7449
                     /locus_tag="VW049_000991"
     CDS             6583..7449
                     /locus_tag="VW049_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9432133.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7446..8204
                     /locus_tag="VW049_000992"
     CDS             7446..8204
                     /locus_tag="VW049_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432134.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8294..10054
                     /gene="dnaG"
                     /locus_tag="VW049_000993"
     CDS             8294..10054
                     /gene="dnaG"
                     /locus_tag="VW049_000993"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9432135.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGNVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10057..11166
                     /gene="rpoD"
                     /locus_tag="VW049_000994"
     CDS             10057..11166
                     /gene="rpoD"
                     /locus_tag="VW049_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9432136.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11180..11509
                     /locus_tag="VW049_000995"
     CDS             11180..11509
                     /locus_tag="VW049_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9432137.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11624..12667
                     /gene="cpoA"
                     /locus_tag="VW049_000996"
     CDS             11624..12667
                     /gene="cpoA"
                     /locus_tag="VW049_000996"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9432138.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12683..14008
                     /locus_tag="VW049_000997"
     CDS             12683..14008
                     /locus_tag="VW049_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9432139.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14039..14215
                     /locus_tag="VW049_000998"
     CDS             14039..14215
                     /locus_tag="VW049_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9432140.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14274..14408
                     /locus_tag="VW049_000999"
     CDS             14274..14408
                     /locus_tag="VW049_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9432141.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14472..15782
                     /gene="obgE"
                     /locus_tag="VW049_001000"
     CDS             14472..15782
                     /gene="obgE"
                     /locus_tag="VW049_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9432142.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15792..15950
                     /locus_tag="VW049_001001"
     CDS             15792..15950
                     /locus_tag="VW049_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432143.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         4941 a         2825 c         3601 g         4731 t
ORIGIN      
        1 tatatattat acttttctca ggagaaagct agatgtacag acgtttgaga gatttgaggg
       61 aagatcacga tttgacccaa aaacaaatag ctaaaatact ttcgtttaca aactcagctt
      121 atgctaaaat tgaacggggt gagcatacgt taacagcaga tgtattggta actctctcaa
      181 acttttacga tgtcagtaca gactacctat tggaattgac agattttcct gataaaattc
      241 gctttagaaa ataatctcct caatttcata gagtgtgaaa atgaggagat tttttatttg
      301 ttctttgaaa attaaatagt ccaaaatggt actttcctca tttgtggagc aaatttgaat
      361 ggctcgccat gataagagcg attttaaaac catcaataaa atagagcgat actttatatg
      421 ccatgataca aatgatatac aatgatactt ctgaccgttc aggctgccat cgtaaaagag
      481 cagcaagtga aattcttatg atgacttcat cagtcatgcc acgttaaatg tgtgagtttg
      541 ttagagaaac gcaattaatc ctcaaaaggt tccccgaacc ttttgagttc cacagacgca
      601 tcacgtggag tgtgtaagct tgttgctaaa agcgtaaaaa ccttggaacg aaaggaataa
      661 tagacttcct gcgaaacaaa aatacaatac aataaaacta tggatgatga agcaagtaaa
      721 caattaagcg atagccgttt caagagcctt gtaggtgttc agcgcacgag atgttagctg
      781 tgtcaaaaac agcttatcaa cgcaacgtaa acgcgcaaaa ggtggacgaa aaaccaaatt
      841 aagcctagac gatctcctca tggtaactat tcaatacatg cgagaatagc gcacttatga
      901 acaaattgcg gctgattttg gcattcacga aagcaactta atccgtcgga gccaatgggt
      961 tgaagtaact cttgttcaaa atggttttac gatttcaaat tctgccctaa ttatgaaaaa
     1021 cagtaaaatt cgaaggattg taaggtaaga gtttttttct ttctgaaaaa atggtataat
     1081 agcaatcaaa actagaaaat aaaacggaat ttggaacaga tttgtctgta tcctagtaga
     1141 gtggtgatgc tatgaagatt agtaagaggc acttattaaa ttattccatc ttgattccct
     1201 acttgctttt atctattttg ggcttgattg tggtctattc gaccaccagt gctattttaa
     1261 ttgaagaagg caagagcgcc ttgcagttgg ttcgaaacca aggaatcttt tggattgtta
     1321 gtttgatact gattgcctta atttataaat tgagactaga ttttttgaga aatgagcgac
     1381 taatcatttt agttatatta atagaaatgc ttttattgtt cttggctcgt tttattggta
     1441 tttccgtaaa cggggcatac ggttggattt cggttgcagg aataactatt cagccagctg
     1501 agtacttaaa aatcattatt atttggtatt tagctcaccg attctccaaa cagcaagaag
     1561 aaatagctac ttatgatttt caagttttga ctcaaaatca atggcttccc cgtgctttta
     1621 atgattggcg attcgttctc ctagttctga ttggaagttt gggaattttc cctgatttag
     1681 gaaatgcgac tattttagtc ttggtttcct tgattatgta tacagttagt ggaatcgctt
     1741 atcgctggtt ttcaaccatt ctggcgctcg tatctgccgc ttctgtcttt gtcttgacca
     1801 ctatcagcct aatcggtgtt gagacctttt caaaaattcc agtatttggc tatgtagcca
     1861 agcgctttag tgcctttttt aatccttttg ccgatcgtgc tgatgcaggt caccagttag
     1921 ctaattctta ttttgccatg gtcaatggcg gttggtttgg tctaggtctt ggaaactcga
     1981 ttgaaaaacg aggttatttg ccagaagctc atacagactt tgtcttttct atcgtgattg
     2041 aagaatttgg ctttgttggt gccagtctta ttttagctct cttgtttttc atgattttgc
     2101 ggattatctt ggtcggtatt cgagcggaga atcctttcaa tgccatggtt gcactcggtg
     2161 tcggagggat gatgttggtt caggtatttg tcaatatcgg agggatttcg ggcttgattc
     2221 catctacagg agtaaccttc cccttcttat cccagggtgg aaatagtctt ctagtcttat
     2281 cagtggcagt agcctttgtc ttaaatattg atgccagtga aaaacgcgct aagttgtacc
     2341 gagaattgga aaatcaacca atgaaccttc tgttgaagta ggataaagaa aggatagttt
     2401 atgtctcttc aaaaattaga aaattatagt aataaaagtg ttgtgcaaga agaagtcttg
     2461 attctaacag aattactgga agatattact aaaaatatgc ttgccccaga gacctttgaa
     2521 aaaataatac agttgaaaga attatcaacg caggaagatt atcaaggtct aaaccgtcta
     2581 gtgactagct tatcaaatga tgaaatggtc tatatttcac gctatttctc tatcttgcct
     2641 cttttgatta atatttcaga ggatgtggat ttagcttatg aaatcaatca tcaaaataat
     2701 attgatcagg actatttagg taaattatct acaacgatta aattggtagc agaaaaggaa
     2761 aatgccgttg agatcctaga acacttgaat gttgtccctg ttttgacagc ccatccaaca
     2821 caagtgcaac gcaaaagtat gttggattta acaaatcata ttcatagtct tttgcgtaaa
     2881 taccgtgatg ttaagttggg gttgatcaat aaagataaat ggtacaatga tttgcgtcgt
     2941 tacatcgaaa ttatcatgca gacagacatg attcgtgaga aaaaattaaa agtgactaac
     3001 gaaatcacga atgctatgga atattataac agctcctttt tgaaagctgt acctcatttg
     3061 acgacggagt ataagcgctt agcgcaagcg catggtctga atttaaaaca ggctaaacca
     3121 atcaccatgg gtatgtggat aggtggtgac cgtgatggaa atccatttgt tacagcaaag
     3181 accttgaagc agtctgcact cactcagtgt gaagtcatca tgaactacta tgataaaaag
     3241 atttaccaac tttatcgtga attttctctt tcaactagca ttgtcaacgt cagcaagcaa
     3301 gtcagagaaa tggctcgtca atccaaggat aactcgattt accgcgaaaa agagctttac
     3361 cgtcgtgcct tgtttgatat tcaatcaaaa attcaggcaa ctaaaaccta tctgattgag
     3421 gatgaagaag ttgggactcg ttatgaaacc gccaatgatt tctacaagga tttgattgcc
     3481 attcgagatt ctctactaga aaataagggt gagtccttga tttcaggtga ttttgtggaa
     3541 ttattgcagg cagtagagat atttggtttt tacttagcgt caattgatat gcgacaagac
     3601 tctagcgtct atgaagcctg tgtggcagaa ctcttgaaat cagcaggaat tcattctcgt
     3661 tatagcgagt tgagcgaaga agaaaagtgt gaccttctct tgaaagaatt agaagaagat
     3721 ccccgaattc tttctgcgac tcacgcagaa aaatcagaat tattagcaaa agaattagct
     3781 atttttaaga cggctcgtgt tttgaaagat aagttgggag atgatgtcat ccgtcagacc
     3841 atcatttcac atgcaaccag cctttctgat atgctagaat tagctattct gttaaaagag
     3901 gtaggactgg tggatacgga aagggcgcgt gttcagattg ttcccctttt tgaaacaatt
     3961 gaagacttgg atcattcaga ggaaacaatg agaaaatatc tttctcttag ccttgccaaa
     4021 aaatggattg actcacgaaa taactaccaa gaaatcatgc ttggctactc tgacagtaat
     4081 aaagatggcg gttacttgtc atcatgttgg accctctaca aggctcaaca acaattgact
     4141 gctattggag atgaatttgg cgttaaggtt accttcttcc atggtcgtgg tggtactgtc
     4201 ggtcgtggtg gtgggccaac ctatgaagcc attacatctc aaccgctcaa gtctatcaag
     4261 gattgtatcc gcttgacgga gcagggtgaa gtaattggga ataaatacgg taacaaagac
     4321 gccgcttact ataaccttga aatgctagta tcggcagcta ttaaccgtat gattactcag
     4381 aagaagagcg ataccaatac cccaaatcgt tatgaagcca ttatggatca agtagtggac
     4441 cgtagttacg atatctaccg tgatttggtc tttggtaatg agcatttcta tgattatttc
     4501 ttcgagtcaa gtccaatcaa ggctatttca agttttaata ttggttctcg tccagccgct
     4561 cgtaagacta ttactgaaat cggtggtttg cgtgccatcc cttgggtatt ctcatggtca
     4621 cagagtcgtg ttatgttccc tggatggtac ggggttggtt caagcttcaa ggaatttatc
     4681 aataaaaatc cagagaatat tgctatctta cgagatatgt accaaaattg gcctttcttc
     4741 caatcgcttc tttcaaatgt tgatatggtt ttgtcaaaat caaatatgaa tattgctttt
     4801 gaatatgcta aactttgtga agacgagcaa gttaaggcca tctatgagac tattttaaat
     4861 gaatggcaag ttactaagaa cgttatcttg gctattgaag gacatgacga actcttagct
     4921 gacaatccat atctaaaagc tagtctggat taccgtatgc cttactttaa tattctcaac
     4981 tatattcagt tagagttgat taaacgccaa cgtcgtggag aattgtccag tgatcaagaa
     5041 cgattgattc atatcaccat caacggaatt gcgacaggat tgcgtaattc aggttgataa
     5101 ttttcaagag tgaatgctaa aagtgaatat caaaaaaatt ctaatagact attgacaagt
     5161 agtttaaaaa tgatataatt taaccattca gaaaagtaat catacaaact ttttagagag
     5221 tctgtggtag ctgaaaacag ataagtggcg atgatgaaaa ttgggctgaa tgctatttag
     5281 aatttgaaat tataaaaatt cggtaagcac accttacagt gcatctcgtt attgcgagac
     5341 tgagcgatag ggaaattccc tataattgag gtggtaccgc gcatcgacgt ccttacacaa
     5401 gttttttgtg tgaggatttt tttgatggag gttagtatgg aaagaaaacg atggcgtcgc
     5461 ttgtttagat aagtgaaata tgttaaagga aataaaaagg agaaacagaa tgaaaaataa
     5521 acgtttaatt ggaattattg ctgcattagc agtcttagta gcaggaagct tgatttattc
     5581 ttcaatgaat aaatcagaag ctcagaataa taaggatgag aagaaaataa ccaagattgg
     5641 tgtgcttcaa tttgtgagcc atccatccct tgatttgatt tataaaggga tccaagatgg
     5701 acttgcagaa gaaggatata aagatgatca agttaaaatt gattttatga actcagaagg
     5761 tgaccaaagt aaggttgcga caatgagtaa acaattggtt gcaaatggga atgaccttgt
     5821 ggttggtatc gcaacatcag cagcccaagg gttggctagt gcaacaaaag acctaccagt
     5881 tatcatggcc gctattacag acccaattgg tgctaacttg gttaaaggtt tgaaaaaacc
     5941 aggtggcaac gttacagggg tatctgacca caatccagct caacaacaaa ttgaactcat
     6001 caaggctctg acaccgaatg tgaaaacaat cggagctctt tactcaagta gcgaagacaa
     6061 ttcaaaaaca caggtcgaag aatttaaggc ttatgctgaa aaagcaggtc tgacagtgga
     6121 aacatttgca gttccttcaa caaatgaaat tgcctcaact gtcactgtta tgactagcaa
     6181 ggtagatgct atttgggttc caattgataa caccattgca tcaggatttc caacggttgt
     6241 ctctagcaat caaagttcta agaaaccaat ttatcccagt gcgacagcta tggtagaagt
     6301 aggtggtttg gcatcagttg taattgacca acatgacctt ggtgtggcaa caggtaaaat
     6361 gattgtgcaa gtcttgaaag gtgcaaaacc agccgatacc ccagtcaatg tcttttcaac
     6421 tggtaagtca gtcatcaata aaaaaatagc acaagaacta ggtattacta ttcctgagtc
     6481 tgttctcaaa gaagcaggac aagtcatcga ataaataagg tagggaggag gagactcttc
     6541 ccatttttta aaaagatgaa tatagaaagg attaaataaa atatgattgt ttccattatt
     6601 tctcaaggat ttgtctgggc tattctaggt ctgggaatct ttatgacatt taggatttta
     6661 aactttccag atatgacgac agaaggttcc ttccctcttg ggggagctgt tgctgtcact
     6721 ttgataacca aaggcgtgaa cccattttta gcgacacttg ttgctgtagg agcaggttgt
     6781 ttggctggaa tggcagcagg ccttctttat acaaaaggga agatcccaac cttgctctca
     6841 gggattttgg tgatgacttc ttgtcactca atcatgctct tgattatggg acgtgcgaat
     6901 ttaggcctgc ttggaaccaa gcaaattcag gatgttttgc cttttgattc ggatttgaat
     6961 caactcttga caggtctcat ctttgtgagt attgttattg ctctcatgct ctttttcttg
     7021 gacactaaac tcggacaagc ctatattgct acaggggata atcctgatat ggctagaagt
     7081 ttcgggattc atactggacg catggagctc atgggcttgg tcttatcaaa tggtgtgatt
     7141 gcccttgcag gtgccctcat tgctcagcaa gaaggttatg ccgatgtgtc tcgagggatc
     7201 ggggttatcg ttgtggggct tgcaagtttg attattggag aagttatttt caagagtttg
     7261 agcttggcag agcgtttggt tactatcgtt gtaggttcta tcgcttatca atttttagtg
     7321 tgggcagtta tcgcacttgg ctttaataca agttaccttc gtttatacag tgccttgatt
     7381 ttagcagtct gcctcatgat tccaacattt aagcaaacaa tcttgaaagg agccaagtta
     7441 agcaaatgac agcaattgta gaattaaaaa atgcaaccaa aatcgttaaa aatggctttg
     7501 atgaagaaaa gattatttta aatgatgttt ccttagaaat ttttgaacgg gactttatca
     7561 cgattttggg cggaaatggt gctggaaaat caactctctt taacactata gcagggacct
     7621 tatcactaac tagtggaact atccgtattt taggtgaaga tctcactaag ttttcacccg
     7681 agaagcgtgc caagtacctg tctcgtgtct tccaagatcc aaagatgggg acagctcccc
     7741 gtatgacggt cgctgaaaat cttttaatcg ccaagtttcg tggtgaaaag cgtggattgt
     7801 taccacgacg cttgactagc tataaggatg aatttcaggc aaccattgaa aaagtaggaa
     7861 atggtcttga gaaacacttg aatacaccga ttgagttctt atcaggtgga caaagacagg
     7921 ctttgagtct cttgatggca accttgaagc gacctgaatt actcctgtta gatgagcata
     7981 ctgctgccct ggatccaaag actagtgttg ctttgatgga attgacagat gaatttgtta
     8041 agaaagatca gctaacagcc cttatgatta ctcatcatat ggaagatgct ctcaaatacg
     8101 gcaatcgctt gattgtcatg aaagaaggac gaattatcca agatttaaac caagaagaaa
     8161 aagcaaaaat gaaaatctct gattattatc aactctttga ataaagtagg gaaaatgagt
     8221 gaaatggttt actttttttc tgaaataaag tatactatat aaagtaaact atgataacat
     8281 ggaggtattg tgtatggttg acaaacaagt cattgaagaa atcaaaaaca atgccaacat
     8341 tgtggaagtc ataggagatg tgatttcttt acaaaaggca ggacggaact atctagggct
     8401 ctgtcctttt catggtgaaa aaacaccatc tttcaatgtt gtagaggaca agcagtttta
     8461 tcactgtttc ggttgtggtc gctcaggtga tgtctttaag ttcatcgagg agtaccaagg
     8521 ggttcccttt atagaagcag tccaaatctt aggtcagcgt gttggaattg aggttgaaaa
     8581 accgctttat agtgagcaga agtcagcctc acctcaccaa gctctttatg atatgcacga
     8641 agatgcagcc aaattttacc atgctattct catgacaacg actatgggcg aagaggccag
     8701 aaattacctt tatcagcggg ggttgacaga tgaagtgctt aaacattttt ggattggttt
     8761 agcacctcca gaacgaaact atctctatca acgtttgtct gatcagtatc gtgaagagga
     8821 tttactggat tcaggcctgt tttatctctc ggatgccaat caatttgtag acacctttca
     8881 caatcgcatt atgtttcccc tgacaaatga ccaaggaaat gtcattgcct tctcaggtcg
     8941 tatctggcaa aaaacggatt cacaaacttc taagtataaa aacagccgat cgactgcaat
     9001 ttttaacaaa agttacgaat tatatcatat ggatagggca aaaagatctt ctggaaaagc
     9061 tagtgagatt tacctgatgg aaggattcat ggatgttatt gcagcctatc gggctggaat
     9121 cgaaaatgct gtggcgtcga tgggaacagc cttgagtcga gagcatgttg agcatctgaa
     9181 aaggttaacc aagaaattgg ttcttgttta cgatggagat aaggctgggc aagccgcgac
     9241 attgaaagca ttggatgaaa ttggtgatat gcctgtgcaa atcgtcagca tgcctgataa
     9301 cttggatcct gatgagtatc tacaaaaaaa tggtccagaa gacttggcct atctattaac
     9361 gaaaactcgt attagtccga ttgagttcta cattcatcag tacaaacctg aaaacagtga
     9421 aaatctgcag gctcagattg agtttcttga aaaaatagct cccttgattg ttcaagaaaa
     9481 gtccatcgct gctcaaaaca gctatattca tattttagct gacagtctgg cgtcctttga
     9541 ttatacccag attgagcaga ttgttaatga gagtcgtcag gtgcaaaggc agaatcgcat
     9601 ggaaggaatt tccagaccga cgccaatcac catgcctgtc accaagcagt tatcggctat
     9661 tatgagggca gaagcccatc tactctatcg gatgatggaa tcccctcttg ttttgaacga
     9721 ttaccgtttg cgagaagact ttgcatttgc tacacctgaa tttcaggtct tatatgactt
     9781 gcttggccag tatggaaatc ttcctccaga agttttagca gagcagacag aggaagttga
     9841 aagagcttgg taccaagttt tagctcagga tttgcctgct gagatatcgc cgcaggaact
     9901 tagtgaagta gagatgactc gaaacaaggc tctcttgaat caggacaata tgagaatcaa
     9961 aaagaaggtg caggaagcta gccatgtagg agatacagat acagccctag aagaattgga
    10021 acgtttaatt tcccaaaaga gaagaatgga gtaataatgg caacaaaaca aaaagaagta
    10081 acaacatttg acgtacaggt agcagaattt atccgtaatc ataagcaaaa agggacagca
    10141 acagatgatg aaatcaatgc aagtctggtt attcctttta ccttggacgc tgatgggatt
    10201 gaagatctct tgcaacggat tcaggatgca ggcatttcta tcacagataa cgaaggaaat
    10261 ccaagtgcgc gtgttctcag caatgaagaa gaaccagaac tcagcgatga ggacttgatt
    10321 gggtcaactt ctgctaaggt caatgaccct gtccgtatgt acttgaaaga aataggggtc
    10381 gttcctctct tgaccaatga agaggagaaa gagttggcac tggctgttga agctggtgat
    10441 atcgaagcca aacaacgtct tgcggaagcc aatcttcgtt tggttgtttc cattgccaaa
    10501 cgctatgtcg gtcgtggtat gcagttcctt gacttgattc aagaaggaaa tatgggcttg
    10561 atgaaggcgg ttgacaagtt tgactattct aaagggttca agttttcaac ttatgcaact
    10621 tggtggattc gtcaggctat cactcgtgct attgcggacc aagctcgtac catccgtatc
    10681 ccagttcaca tggttgaaac tatcaataaa ttggttcgtg aacagcggaa tctccttcaa
    10741 gaattggggc aagatccgac accagaacag attgctgaac gaatggatat gacacctgat
    10801 aaggttcgtg aaatcttgaa gattgcccaa gaaccagtat ctcttgaaac tcctatcggt
    10861 gaagaggacg atagccacct tggagacttt atcgaagatg aagtgattga aaatccagtg
    10921 gattatacga ctcgtatcgt cttgcgtgag caattggatg aaatcttaga tactcttaca
    10981 gaccgtgaag aaaatgttct gcgtctacgt tttggactag atgatggaaa aatgcgcaca
    11041 cttgaagatg tggggaaagt ctttaacgta actcgtgagc gtatccgtca gattgaagca
    11101 aaggctttga gaaaactacg ccaaccaagt cgtagcaaac cgcttcgtga ttttattgaa
    11161 gactaagagt gaggaaaata tggcttatac agaagagcaa attgaaaaca tcaaaacacg
    11221 gattttaaca gccttggaag aagtcatcga ccctgagttg ggaatcgata ttgtcaatct
    11281 tggtttgatc tatgagattc gttttgacgg tgacacaggg caaacagaga ttgacatgac
    11341 tttgacaact atgggttgtc ccttagcaga ccttttgaca gaccagattt atgatgctat
    11401 gatagaggtt ccagaagtaa cggatactga ggttaaatta gtctggtatc cagcctggac
    11461 agttgaaaaa atgagtcgct atgcccgcat tgccctaggc attaagtaaa catataaaag
    11521 catgtgagag actgttggaa aagcgaggaa atttcccctc ttttcctcta gtctctcctt
    11581 tcttttgctg attttattca aagaaaatga tataatagta gttatggaga aaaagaaatt
    11641 acgcatcaat atgttgagtt caagtgagaa agtagcagga cagggagttt caggtgctta
    11701 ccgtgaatta gttcgtcttc ttcaccgtgc tgccaaggac caattgattg ttacagaaaa
    11761 tcttccaatc gaggcagatg tgactcactt tcatacgatt gattttccct attatttatc
    11821 aaccttccaa aagaaacgct cagggagaaa gattggctat gtgcatttct tgccagctac
    11881 acttgaggga agtttgaaaa ttccattttt cttaaaggga attgtgaaac gctatgtatt
    11941 ttctttttac aaccggatgg agcacttggt tgtggtcaat cctatgttta ttgaggattt
    12001 ggtagcagct ggtattccac gtgaaaaagt gacctatatt cctaactttg tcaacaagga
    12061 aaaatggcat cctctaccac aagaagaggt agtcagactg cgcacagatc ttggtcttag
    12121 tgacaatcag tttatcgtag taggtgctgg gcaagttcag aaacgtaaag ggattgatga
    12181 ctttatccgt ctggctgagg aattgcctca gattaccttt atctgggctg gtggcttctc
    12241 ttttggtggt atgacagatg gttatgaaca ctataagaaa attatggaaa atccccctaa
    12301 aaatttgatt tttccaggca ttgtatcgcc agagcggatg cgcgaattgt atgctctagc
    12361 ggatcttttc ttgttgccta gttacaatga gctctttcct atgactattt tagaagctgc
    12421 gagttgtgag gctcctatta tgttgcgtga tttagatctc tataaggtga ttttggaggg
    12481 aaattatcgg gcgacagcgg gtagagaaga gatgaaagag gctattttgg aatatcaagc
    12541 aaatcctgct gtcttaaaag atctcaaaga aaaggctaag aatatttcca gagagtattc
    12601 tgaagagcat ctgttacaaa tctggttgga cttttatgag aaacaagccg ctttagggag
    12661 aaagtaaaaa gtgaggtaat ctatgcgaat tggtttattt acagatacct attttcctca
    12721 ggtttctggt gttgcgacca gtattcgaac cttgaaaaca gaacttgaaa agcagggaca
    12781 tgctgttttt atctttacga cgacagataa ggatgtcaat cgctacgaag attggcaaat
    12841 tatccgcatt ccaagtgttc ctttctttgc ttttaaggat cgtcgctttg cctaccgagg
    12901 ttttagcaag gcacttgaaa ttgctaaaca gtatcagcta gatattatcc atactcagac
    12961 agaattttct cttggcctgt tggggatttg gattgcgcgt gaattgaaaa ttccagtcat
    13021 ccatacctat cacacccagt atgaagacta tgtccattat attgctaagg ggatgttgat
    13081 ccggccgagt atggtcaagt atctggttag aggtttcctg catgatgtgg atggggttat
    13141 ttgccctagt gagattgtcc gtgacttgct atctgattat aaggtcaagg ttgaaaaacg
    13201 ggtcattcct actgggattg aattagccaa gtttgagcgt ccggaaatca agcaggaaaa
    13261 tttgaaagaa ctgcgtagta aactagggat tcaagatggt gaaaagacgt tgcttagtct
    13321 ttcgagaatc tcctatgaaa aaaatattca agcagtttta gcagcctttg ctgatgttct
    13381 gaaagaggaa gacaaggtta aactggtagt agctggggat ggcccttatc tgaatgacct
    13441 caaagagcaa gcccagaacc tagagattca agactcagtc atctttacag ggatgattgc
    13501 tcctagtgag acggctcttt actataaagc ggcggatttc ttcatttcgg catcgacaag
    13561 cgaaacgcaa ggtttgacct acttggaaag cttagccagt ggaacacctg tcattgctca
    13621 cggaaatcct tatttgaaca acctcatcag tgataaaatg tttggaacct tgtactatgg
    13681 agaacatgat ttggctggtg ctattttgga agccctgatt gcaacaccag acatgaacga
    13741 gcatacctta tcagagaaat tgtatgagat ttcagctgag aactttggga aacgagtgca
    13801 tgagttttat ctggatgcca ttatttcaaa taacttccag aaagatttgg ctaaagatga
    13861 tacggtcagt cagcgtatct ttaagacagt tttgtatctt cagcaacagg tggttgctgt
    13921 acctgtaaaa ggatctagac gcatgttgaa ggcttcaaaa acacagttga tcagtatgag
    13981 agactattgg aaagaccatg aagaatagaa agaggaacag ctatgaaaaa aacaattaat
    14041 gagaagcggt cgtgataaaa agattgcggg tgtttgtgct ggggtggccc attatctgga
    14101 tatggatccg actatcgttc aagtcatttg gggtgttctt acttgctgtt acggagctgg
    14161 aattgtagct tacattattt tatggattat cgcaccagta gcaactgact attgaaaact
    14221 agctcaattc atgctaagat aatagaaaat agaatgtaac gaaggagaga aaaatggcat
    14281 ttggagataa tggaaatcgt aaaaaaacta tgtttgagaa aataaccttg tttatcgtga
    14341 ttatcatgct agtagcaagt ttattgggaa tttttgcaac tgcaattggt gccctcagta
    14401 atctataaaa tagattcaag aaaatttagt gactgggatt tcccagccct tttttaaagt
    14461 gagaagaaat aatgagtatg tttttagata cagctaagat taaggtcaag gctggtaatg
    14521 gtggcgatgg tatggttgcc tttcgtcgtg aaaaatatgt ccctaatgga ggcccttggg
    14581 gtggtgatgg tggtcgtgga ggcaatgtgg tcttcgttgt agacgaagga ctacgtacct
    14641 tgatggattt ccgctacaat cgtcatttca aggctgattc tggtgaaaaa gggatgacca
    14701 aagggatgca tggtcgtggt gctgaggacc ttagagttcg agtaccacaa ggtacgactg
    14761 ttcgtgatgc ggagactggc aaggttttaa cagatttgat tgaacatggg caagaattta
    14821 tcgttgccca cggtggtcgt ggtggacgtg gaaatattcg tttcgcgaca ccaaaaaatc
    14881 ctgcaccgga aatctctgaa aatggagaac caggtcagga acgtgagtta caattggaac
    14941 taaaaatctt ggcagatgtc ggtttagtag gattcccatc tgtaggaaag tcaacacttt
    15001 taagtgttat tacctcagct aagcctaaaa ttggtgccta ccactttacc actattgtac
    15061 caaatttagg tatggttcgc acccaatcag gtgaatcctt tgcagtagcc gacttgccag
    15121 gtttgattga aggggctagt caaggtgttg gtttgggaac tcagttcctc cgtcacatcg
    15181 agcgtacacg tgttatcctt cacatcattg atatgtcagc tagcgagggc cgtgatccat
    15241 atgaggacta cctagctatc aataaagagc tggagtctta caatcttcgc ctcatggagc
    15301 gtccacagat tattgtagct aataagatgg acatgcctga gagtcaggaa aatcttgaag
    15361 actttaagaa aaaattggct gaaaattatg atgaatttga agagttacca gctatcttcc
    15421 caatttctgg attgaccaag caaggtctgg caacactttt agatgctaca gctgaattgt
    15481 tagacaagac accagaattt ttgctctacg acgagtccga tatggaagaa gaagcttact
    15541 atggatttga cgaagaagaa aaagcctttg aaattagtcg tgatgacgat gcgacatggg
    15601 tactttctgg tgaaaaactc atgaaactct ttaatatgac caactttgat cgtgatgaat
    15661 ctgtcatgaa atttgcccgt cagcttcgtg gtatgggggt tgatgaagcc cttcgtgcgc
    15721 gtggagctaa agatggggat ttggtccgca ttggtaaatt tgagtttgaa tttgtagact
    15781 aggagactgg tatgggagat aaaccgatat ctttccgaga tgcggatggt aattttgttt
    15841 ccgccgcaga cgtttggaat gaaaagaaat tggaagaact atttaatcgt ctcaatccaa
    15901 atcgtgcctt gagattggca cgaactaaaa aggaaaatcc atctcagtaa agaagctaaa
    15961 aaatcccgtg cctcatcaga cacgggattt tgtggtacga caggcatgta tagcaaactg
    16021 aatctggaat agcacagcat atcttctaaa atatagtaaa atgaaatgag aacaggacaa
    16081 atcgatcagg acagtaaa
//
LOCUS       DAVKGC010000037        16001 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKGC010000037 DAVKGC010000000
VERSION     DAVKGC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16001
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.037"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            138..686
                     /locus_tag="VW049_001002"
     CDS             138..686
                     /locus_tag="VW049_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9432144.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            796..1695
                     /locus_tag="VW049_001003"
     CDS             796..1695
                     /locus_tag="VW049_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9432145.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFCVLGAYLIWLRKKV"
     gene            complement(1720..2994)
                     /gene="serS"
                     /locus_tag="VW049_001004"
     CDS             complement(1720..2994)
                     /gene="serS"
                     /locus_tag="VW049_001004"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9432146.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3207..3578)
                     /locus_tag="VW049_001005"
     CDS             complement(3207..3578)
                     /locus_tag="VW049_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9432147.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3581..4945)
                     /locus_tag="VW049_001006"
     CDS             complement(3581..4945)
                     /locus_tag="VW049_001006"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9432148.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREIGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5314..6099
                     /locus_tag="VW049_001007"
     CDS             5314..6099
                     /locus_tag="VW049_001007"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9432149.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRVSSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6193..6627
                     /locus_tag="VW049_001008"
     CDS             6193..6627
                     /locus_tag="VW049_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9432150.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6627..7601
                     /locus_tag="VW049_001009"
     CDS             6627..7601
                     /locus_tag="VW049_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9432151.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7661..7885
                     /locus_tag="VW049_001010"
     CDS             7661..7885
                     /locus_tag="VW049_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9432152.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            8004..8978
                     /gene="fabK"
                     /locus_tag="VW049_001011"
     CDS             8004..8978
                     /gene="fabK"
                     /locus_tag="VW049_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9432153.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            8971..9891
                     /gene="fabD"
                     /locus_tag="VW049_001012"
     CDS             8971..9891
                     /gene="fabD"
                     /locus_tag="VW049_001012"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9432154.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9925..10656
                     /gene="fabG"
                     /locus_tag="VW049_001013"
     CDS             9925..10656
                     /gene="fabG"
                     /locus_tag="VW049_001013"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9432155.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQVGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            10678..11913
                     /gene="fabF"
                     /locus_tag="VW049_001014"
     CDS             10678..11913
                     /gene="fabF"
                     /locus_tag="VW049_001014"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9432156.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11916..12401
                     /locus_tag="VW049_001015"
     CDS             11916..12401
                     /locus_tag="VW049_001015"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9432157.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12398..12820
                     /gene="fabZ"
                     /locus_tag="VW049_001016"
     CDS             12398..12820
                     /gene="fabZ"
                     /locus_tag="VW049_001016"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9432158.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            12832..14199
                     /gene="accC"
                     /locus_tag="VW049_001017"
     CDS             12832..14199
                     /gene="accC"
                     /locus_tag="VW049_001017"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9432159.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14236..15102
                     /locus_tag="VW049_001018"
     CDS             14236..15102
                     /locus_tag="VW049_001018"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9432160.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            15099..15866
                     /locus_tag="VW049_001019"
     CDS             15099..15866
                     /locus_tag="VW049_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9432161.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
BASE COUNT         4580 a         3040 c         3747 g         4634 t
ORIGIN      
        1 attggaagtg tgataaacta atataacaac gaaaatctta tgagagaata aagctttatc
       61 ttagataaac ttaggaatca ggattttata agattttcca ataatattag tgtcaataat
      121 aagaaaagag gtatcttatg acaacattta ctatccatac agtagagtca gcaccagcag
      181 aagtgaaaga aattcttgaa acagtagaaa aagacaacaa tggctatatt cccaacctaa
      241 tcggtctctt ggccaatgcc ccgactgttt tagaagccta ccaaattgtc tcatctatcc
      301 accgtcgcaa cagcctgaca cccgttgagc gtgaagtggt gcaaatcacg gcagccgtga
      361 ccaatggttg tgccttctgt gtcgcaggtc acacagcctt ttccatcaaa caaatccaga
      421 tgaatgatga cttgattcaa gctcttcgca atcgtactcc aattgaaaca gatcctaaat
      481 tggataccct agctaagttt accttggcag ttatcaatac caagggtcgt gtaggagatg
      541 aagccttgtc tgagttttta gaagctggct acactcaaca aaatgccttg gatgtggttt
      601 ttggtgtcag cctagcaatc ctctgtaact atgccaacaa cttagctaat acaccaatta
      661 atccagaatt gcaaccttat gcctaattca tatctgagta aaatgaagtc tgaaataagc
      721 ggacttcgtt tttttaaatc ctcatttaag cgcttttatg ttatactgaa actaacatga
      781 ttattggagg ttaggatgaa aaaactcccc ttagtatttt caggttgttt gctaggtttg
      841 gcaggagctg gaaatcttat tttagatacg ttgccggttc tatcccatct ttttagtctg
      901 actggtttgg ttttatggat ttactttcta attctgcatc tctttaattg gaaagaaacc
      961 aagcaagaat tgaccaagcc ccctcttttg tcaggaatgg caacctttcc tatggctggg
     1021 atgattttat cgacctatgt ctttcgcgta ttctcttatc ttcctttggt agcacaaggg
     1081 atttggtggt tttcatttct cttggatttg accttgattg ctggttttac catcaagttt
     1141 gcttgtccag ggcggagggt tcatgccact ccaagttgga cggttctcta tgtggggata
     1201 gcagtggctg ccttgaccta tcctctggta ggtattatcg aaattgccta tgcgaccttg
     1261 agttttggtt ttctcttgac cttctatctc tatcccctta tttatagcga tttaaagaaa
     1321 catccactcc cactagcctt gcttggacaa gaaggaatct actgtgctcc tttctctcta
     1381 ctcttggctt ctctagttcg agtaggagga accagcctgc cgacttgggt cttgattgtc
     1441 atgattttgg cttctcaatc cttctttttc tttgttttaa ctcgtctgcc caacatttta
     1501 aaacaaggtt ttcaaccagc cttctcagcc ctcaccttcc caaccattat cacagcgacc
     1561 tcgctcaaga tggctcaggg aattttgaaa cttccatttc tggattacct ggtattggct
     1621 gaaaccatta tatgcctaac tattttattc tgtgtactag gtgcttatct gatttggtta
     1681 cgaaaaaagg tctagctaga aatagctaaa ccttattttt tatggtttga tgacttcagc
     1741 tccacccatg tatggacgaa gtgcttctgg gatggtcaca gaaccatctt cattttggta
     1801 attttcaaga attgcagcca ctgtacgtcc aactgcaagt ccagaaccgt tcaaggtatg
     1861 aaggagtttc accttgccat ctgcttcatc acggtaacgg atttgggcac gacgggcttg
     1921 gaaatcttct gtgtttgaac agcttgagat ttcacggtaa ttgttttgtg ctggaatcca
     1981 cacttccaag tcgtaagtct tcgtagctga gaagcccata tctccagtag agagagcaac
     2041 gacacggtat ggaaggttga gtttttgaag aatgttttca gcgttggctg tcattttttc
     2101 caattcttcg taagattctt ctggtttggc aaatttgacc atttcaacct tgtggaattg
     2161 gtgcaaacgg atcaagccac gcgtatcacg accggcagaa ccagcctcag aacggaatga
     2221 cggactcata gcagtgaagt agattggaag atctttgccg tctaagattt catcacggta
     2281 gtagtttgtc agaggaactt cagcagttgg aatcaagaca aagttggtat cgctgagttc
     2341 aaaagtatct tccttaaatt ttggatactg accagtacca aacatagaat catggttgac
     2401 tatgtaaggt gtgatgactt cagtatagcc ttcttttcca tgttcatcca acataaagtt
     2461 gtagatagca cgttccaaac gagcaccgag gcctttatag aagaggaagc gagcgcctgt
     2521 taccttacca ccgcgttccc agtcaaggat accaaggtct tcaccgagat cccagtgagc
     2581 tttaggttcg aagtcaaact cgcgtggagt accccaacgg cgaacttcca cattgtcgtc
     2641 ttcgtcagcc ccaacaggaa cgctgtcagc tgggatattt ggaagagtcg ttgtaaattc
     2701 tgtcaattta gcatcgattt ctgccaattc agcatccaag gctttaacct cagcagatag
     2761 attttgcatg gcagcaatct tgtcatctgt attttccttg ttgcgcttag cttgggcaat
     2821 ctcagcagaa actgtgttac gttctgcttt gagagtttca accttgacca agatgttacg
     2881 acgtttagca tcgatttctt tcatttcatt caagacagca gcatctacac cacgtgtagc
     2941 taatttttct gcgacagctt caaaatctgt acgaatacgt ttgatatcta acataagaac
     3001 tcctttatga aaaaagcacc ctgacaaaac gttggagtgg cagggccacg gttccatcca
     3061 acttcacagg tgtgcacttg attgtgtatg taattgtcac taacggtaga atttcaccta
     3121 tccctcctat ctgctcgcag cacccgcaga ctttctgaaa gaagaagata acctacttat
     3181 ccgttgctat gattatacta aagtttctac ttttttgcaa atagattttt aaatttttgg
     3241 ctaattgtct gaatcagggt cggaagtttg acgaccttgt cattgcctag tttttcgcgt
     3301 gcaattttga gaatggcacc tgagtctttt gaagcaaaga ggaattttcc tttgtctgta
     3361 aagacttcga agtggcggct gattttgcgt ccagtgacat tggctccaat ctgattgata
     3421 tggctccaag gaatctggat aaattgttcg acattgacat ctgggtaaaa ttccaaagcc
     3481 tgatctccga caaggaattt cccaactttc ccagcgatag agaggtagga agtgcctgtc
     3541 gtactgagga gtactgtttt gttaagtgat tgggccatgc ttagtcttcc ttactttctc
     3601 caaaaaaggc attgtagagg gctttaattg ctgctttctc ttggtcttta ttgacaacaa
     3661 acataataga aacttcacta gaaccttgag acatcatctg gatgttgatt ttgttttcag
     3721 atagagcgcg tgtcgcagta gcagtcactc cgatatggct cttcattttt tcaccaacaa
     3781 tcataatgat agaaaggtcg tgttcgattt ctgcatgatc tactttagcc ttttgaacca
     3841 actgacgcag gatttcttct tccttgatgg gagttagttg gcgagaacgg agaatgatag
     3901 aaagatcgtc gatacctgtt ggcatatgtt cccaaccgat gttaagttct tccaggattt
     3961 gcagaacctt gcggccaaat ccaatctcac ggttcatgag gtatttcgac atattaatgc
     4021 tgacaaagcc tgagtcacca gcaattccca caactggaaa ttcatcatta ctgtgtttta
     4081 gaacgatacg agtacctgga tggtcagggt tgttggtatt cttgataacc agaggaattt
     4141 ttccacggta ggcaggaaga agagcctcgt catgaaggac tgagaagcct gcataggcca
     4201 actcgcgcat ttcacggtag gtcaactcag gaatcgagtg tggttggtgg ataataccag
     4261 ggtgggctgc aaagatacca tcaacgtccg taaagttttc atagaggtca gctttgacac
     4321 cagcagcaat gatagaacct gtaatatcag atcctccacg tgagaaagta cagatttgat
     4381 tttccttagt gacaccaaag aaaccaggaa tgacaaggac ttcattggtg tttgtcaatt
     4441 cttcaatctt gtcataactt gatggaatga tgcgagcgtg accaggttca cttgtgacca
     4501 caatcccagc ttctctaggg tgcatatagc gtgcatcgat accattttgg ttaaagtagg
     4561 cagcaatcaa tttggcattg ttattttcac cggctgctag gaaagtatcg tagagaaatt
     4621 cattttcttc aataggaaga gtggccaagg cgtgaatgct tttagaaatt ttttctagca
     4681 cagctggttt tagtcccaat tcactaacca tagcagcata gcggtcgata atccagcttt
     4741 ggctcttgct aatatcgtta cccgcaacat agtcgcggta gtatttaatc agggcatccg
     4801 taaccttagt atcttcagca ttgcgtttac caggcgcaga aacgactaca aaacgacgct
     4861 ctgaatcgct tttgacgatg tttaaaactt tttctaattg actagcagag gcaagagaac
     4921 tacctccaaa tttaacaact ttcataagaa ctcctaaagt aagtatttta tacgattata
     4981 gcagaaagaa aggctttttt caatgaagaa aataagatgc tttctagcct aaagcgagcc
     5041 tttcttatcg gctgagacac tttcagtcga ttttttcttg ctttctgctc ctaaaaaaga
     5101 tatactttga cagtgaaata atttaacact caatgaaatc aaagagcaaa ctaggaagct
     5161 agccgcagac tgctcaaagc actgctttga ggttgtaaat aagactgacg aagtcagctc
     5221 aaagcactgc tttgaggttg cagatggaag ctgacgcggt ttgaagagat tttcgaagag
     5281 tataaactgc ttataaaata aaaaggagcc ctgatggaac acattattta tcagcttgaa
     5341 gaggacttgg caatccttac cttgaaccgt cctgaggtcg caaatggttt tcatattccc
     5401 atgtgtgagg agattttaga agctctgact ttggcagaag aaaatccagc tgtgcatttt
     5461 atcttaatca atgccaatgg aaaggtcttc tcagttgggg gagatttggt agagatgaag
     5521 cgggcagtgg atgaggatga tattccatca ttgacaaaaa tcgcagagtt ggtcaatacg
     5581 atttcttata aaatcaagca aatagccaaa cctgttttaa tggaagttga cggtgctgtt
     5641 gcaggtgccg cagcgaatat ggctgttgcg gcagatttct gtttggcgac ggataaggct
     5701 aagtttatcc aagcctttgt tggtgttggt ttggctccag atgcaggggg aattcatctc
     5761 ttgagccgca gtatcggtgt gacgcgtgct gctcaattag ctatgacagg agaggcttta
     5821 acggcagaaa aagccctaga atgggggttg gtttaccgcg tttctgaagc tgaaaaactt
     5881 gaaaagacga gagaacagct tcttaaaaaa ttaagacgtg tttcaagtaa ttcctatgct
     5941 gccattaaga agttggtttg ggagagccaa tttaaagact ggcagggtta cgctacttta
     6001 gaactgaacc tacagaaatc cttagctcaa acagaggatt tcaaagaagg agtgcgagct
     6061 cattcggaga gaagaagacc taaatttaca ggaaaataaa aaatacttgc atcattcttt
     6121 gaattttgat ataattcttt ctgtcaaatg ttttgattgt aaaagttttt tgaagaagga
     6181 gggatatgaa aaatggacta ccaacgaatc aatgaatatt taacatctat atttaacaat
     6241 gtccttgtaa ttgaggaagt gaacttgaga ggtagtcgtt ttaaggatat ctccatcaaa
     6301 gaaatgcata cgattgatgt catcggtaag gctccagacg tgactccaag tcaagtgtca
     6361 aaagagttga tggtaactct tggaactgtt acgacaagtt tgaacaattt agagcgtaag
     6421 ggctacattg agcgagttcg gtcagaacag gatcgtcgtg tggtgcatct gcatttgaca
     6481 aagaagggtc gcttgattca taggctgcat aaacgcttcc acaaggccat ggtagaaaaa
     6541 attattgatg gcatgagcga ggaagaaatt gctgtcatgg gtaaaggttt gactaatctt
     6601 taccaatttt tggaggattt gaaataatgg cttttgcaaa aataagtcag gttgctcatt
     6661 atgtgccaga gcaagtggtt acaaatcacg acttggctca gattatggat accaatgatg
     6721 agtggatttc aagtcgaacg ggaatacgac aaaggcatat ttcaagaaca gaatctacca
     6781 gtgatttggc tacagaggtt gctaagaaac tgatggcaaa agctggaata acaggaaaag
     6841 aactggattt tatcatccta gctaccatta ctccagattc gatgatgccc tctacagctg
     6901 ctcgtgttca agctaatatt ggcgctaata aagcctttgc ttttgactta accgcggctt
     6961 gcagtggatt tgtatttgct ctttcaactg ctgaaaagtt tatcgcttct ggtcgctttc
     7021 aaaaaggctt ggtgattggt agtgaaaccc tctctaaggc agtcgattgg tcggatcgat
     7081 caacagctgt gttgtttgga gatggtgctg gtggtgtctt gttagaagct agcgagcaag
     7141 agcatttctt agctgagagt ctcaatagcg atggaagtcg cagcgagtgt ttaacttatg
     7201 ggcattcagg tttgcattct ccattttcag atcaagaaag tgcagattcg tttttgaaga
     7261 tggatggacg cacagtcttt gattttgcca ttcgagatgt agccaagtct atcaagcaga
     7321 ctattgatga atctcctata gaggtgacag acttggatta tctgctactt catcaagcca
     7381 atgaccgtat tttggataag atggctagaa aaattggtgt tgaccgagcc aaacttccag
     7441 ccaatatgat ggaatatggc aataccagtg cagccagtat cccgatttta ctttcagagt
     7501 gtgtagaaca aggtctcatc cctttagatg gtagccagac tgttcttcta tcaggcttcg
     7561 gtggaggctt gacctggggc acgctcattc ttacaattta ggtaatcatg tggtgaacac
     7621 attgttataa aaaaactatt tattttaaag gagtcctatc atggcagtat ttgaaaaagt
     7681 acaagaaatt atcgttgaag aacttggaaa agacgcatca gaagtaacac ttgaatcaac
     7741 ttttgatgat ttggacgcag attcattgga cttgttccaa gtaatctcag aaatcgaaga
     7801 tgcttttgat atccaaatcg aagcagaaaa tgacttgaaa acagttggtg acttggttgc
     7861 ttacgttgaa gagcaagcaa aataagcaga atattagtag aaggagtagg gagaaaccca
     7921 ctccctcttg tttaggtaat agtttgactg tcaaattatg gtgaaatcga actattacgt
     7981 aagcaaggaa ggttggaggc actatgaaaa cgcgtattac agaattattg aagattgact
     8041 atcctatttt ccaaggaggg atggcctggg ttgctgatgg tgatttggca ggggctgttt
     8101 ccaaggctgg aggattagga attatcggtg ggggaaatgc cccgaaagaa gttgtcaagg
     8161 ccaatattga taaaatcaaa tcattgactg ataaaccctt tggggtcaac atcatgctct
     8221 tatctccctt tgtggaagat atcgtggatc tcgttattga agaaggtgtt aaagttgtca
     8281 caacaggagc aggaaatcca agcaagtata tggaacgttt ccatgaagct gggataatcg
     8341 ttattcctgt tgttcctagt gtcgctttag ctaaacgcat ggaaaaaatc ggtgcagacg
     8401 ctgttattgc agaaggaatg gaagctgggg ggcatatcgg taaattaaca accatgacct
     8461 tggtgcgaca ggtagccaca gctatatcta ttcctgttat tgctgcagga ggaattgcgg
     8521 atggtgaagg tgctgcggct ggctttatgc taggtgcaga ggctgtacag gtggggacac
     8581 ggtttgtagt tgcaaaagag tcgaatgccc atccaaacta caaggagaaa attttaaaag
     8641 caagggatat tgatactacg atttcagctc agcactttgg tcatgctgtt cgtgctatta
     8701 aaaatcagtt gactagagat tttgaactgg ctgaaaaaga tgcctttaag caggaagatc
     8761 ctgatttaga aatctttgaa caaatgggag caggtgccct agccaaagca gttgttcacg
     8821 gtgatgtgga tggtggctct gtcatggcag gtcaaatcgc agggcttgtt tctaaagaag
     8881 aaacagctga agaaatccta aaagatttgt attacggagc cgctaagaaa attcaagaag
     8941 aagcctctcg ctgggcagga gttgtaagaa atgactaaaa cagccttttt atttgctggt
     9001 caaggtgccc agtatctagg gatgggacgg gatttctatg atcagtatcc gattgtcaaa
     9061 gaaacgattg atcgagcgag tcaggtgctc ggttatgatt tacgttatct catcgatacg
     9121 gaagaagaca aactcaatca gacccgctat acgcaaccag ccattctagc gacttcggtt
     9181 gctatctacc gtttattgca agaaaagggc tatcagcctg atatggttgc tggtttgtct
     9241 cttggagaat actctgcctt ggtggcaagc ggcgccttgg attttgaaga tgcggttgcc
     9301 ttggtagcta agcgtggagc ctatatggaa gaagcggctc ctgctgactc tggcaagatg
     9361 gtagcagttc tcaatacgcc agtagaggtc attgaagaag cctgtcaaaa agcttctgaa
     9421 cttggagtgg ttactccagc caactataac acacctgcac aaatcgtcat tgctggagaa
     9481 gtggttgcag ttgatcgagc ggttgaactt ttgcaagaag caggtgccaa acgcttgatt
     9541 cctcttaagg tgtcaggtcc ctttcacacc gctctccttg agcctgctag ccagaaacta
     9601 gctgaaactc tagctcaggt aagtttttca gattttactt gtcccctagt cggcaataca
     9661 gaagctgctg tgatgcaaaa agaggacatt gctcagctct tgacgcgtca ggtcaaggaa
     9721 cccgttcgtt tctatgaaag tattggggtc atgcaagaag caggcataag caactttatc
     9781 gagattggac cggggaaagt cttgtcaggt tttgttaaaa aaattgatca aactgctcac
     9841 ttagctcatg tggaagatca agcgagttta gtagcacttt tagaaaaata gactaaaata
     9901 agtagaagtt ttgaaaggaa aaaaatgaaa ctagaacata aaaatatctt tattacaggt
     9961 tcgagtcgtg gaattggtct tgccatcgcc cacaagtttg ctcaagtagg agccaacatt
    10021 gtcttaaaca gtcgtggggc aatctcagaa gaattgctcg ctgagttttc aaactatggt
    10081 atcaaggtgg ttcccatttc aggagatgta tcagattttg cagacgctaa gcgtatgatt
    10141 gatcaagcta ttgcagaact gggttcagta gatgttttgg tcaacaatgc agggattacc
    10201 caagatactc ttatgctcaa gatgacagaa gcagattttg aaaaagtgct caaggtcaat
    10261 ctgactggtg cctttaatat gacacaatca gtcttgaaac cgatgatgaa agccagagaa
    10321 ggtgctatca ttaatatgtc tagtgttgtt ggtttgatgg ggaatattgg tcaagctaac
    10381 tatgctgctt ctaaggctgg cttgattggc tttaccaagt ctgtggcacg cgaggtcgct
    10441 agtcggaata tacgagtcaa tgtgattgct ccaggaatga ttgagtctga tatgacagct
    10501 atcttatcag ataagattaa ggaagctaca ctagctcaga ttccgatgaa agaatttggg
    10561 caggcagagc aggttgcaga tttgacagta tttttagcag gccaagatta tctaactggt
    10621 caagtgattg ccattgatgg tggcttaagt atgtagcgaa agctagaggt gaaaagaatg
    10681 aaactaaatc gagtagtggt aacaggttat ggagtaacat ctccaatcgg aaatacacca
    10741 gaagaatttt ggaatagttt agcaactggg aaaatcggca ttggtggcat tacaaaattt
    10801 gatcatagtg actttgatgt gcataatgcg gcagaaatcc aagattttcc gttcgataaa
    10861 tactttgtaa aaaaagatac caaccgtttt gataactatt ctttatatgc cttgtatgca
    10921 gcccaagagg ctgtaaacca tgccaatctt gatgtagagg ctcttaatag ggatcgtttt
    10981 ggtgttatcg ttgcatctgg tattggtgga atcaaggaaa ttgaagatca ggtacttcgc
    11041 cttcatgaaa aaggacccaa acgtgtcaaa ccaatgactc ttccaaaagc tttaccaaat
    11101 atggcttctg ggaatgtagc catgcgtttt ggtgcaaacg gtgtttgtaa atctatcaat
    11161 actgcctgct cttcatcaaa tgatgcgatt ggggatgcct tccgctccat taagtttggt
    11221 ttccaagatg tgatgttggt gggaggaaca gaagcttcta tcacaccttt tgccatcgct
    11281 ggtttccaag ccttaacagc tctctctact acagaggatc caactcgtgc ttcgatccca
    11341 tttgataagg atcgcaatgg gtttgttatg ggtgaaggtt cagggatgtt ggttttagaa
    11401 agtcttgaac acgctgaaaa acgtggagct actatcctgg ctgaagtggt tggttacgga
    11461 aatacttgtg atgcctacca catgacttct ccacatccag aaggtcaggg agctatcaag
    11521 gccatcaaac tagccttgga agaagctgag atttctccag agcaagtagc ctatgtcaat
    11581 gctcacggaa cgtcaactcc tgccaatgaa aaaggagaaa gtggagctat cgtagctgtt
    11641 cttggtaagg aagtacctgt atcatcaacc aagtctttta caggacattt gctgggggct
    11701 gcgggtgcag tagaagctat cgtcaccatc gaagctatgc gccataactt tgtaccaatg
    11761 acagctggga caagtgaagt atcagattat atcgaagcta atgtcgttta tggacaaggc
    11821 ttggagaaag aaattccata cgctatttca aatacttttg gttttggagg ccacaatgca
    11881 gttcttgctt tcaaacgttg ggagaataga taagtatgaa tttaaacgat attaaagact
    11941 tgatgactca atttgaccag tcaagtttga gagaattttc ttataaaaat gggacggatg
    12001 agttgcagtt tagcaagaat gaagcgagac ctgtgcctga agttgcaact caagtcgctc
    12061 cagcacccgt tctagcaaca ccgagtccag tagctcctac atctgctcca gcagagactg
    12121 tagcagaaga agttccagct ccagctgaag caagtgtggc tactgaggga aatcttgtag
    12181 agagtccact tgttggagtg gtttacttgg ctgctggacc agataaacct gccttcgtta
    12241 cagttggtga tagtgtcaaa aaaggtcaaa cattggtaat tatcgaagcc atgaaagtca
    12301 tgaatgaaat cccagctcct aaggatggtg tggtaacgga aattctcgtc tctaacgaag
    12361 aaatggttga gtttggtaaa ggattggtac gtatcaaatg atcgatattc aaggaatcaa
    12421 agaagctctt ccccaccgtt atcctatgct tctagtggac cgtgtcttgg aagtgagcga
    12481 ggataccatt gttgctatca aaaatgtgac cattaacgaa cctttcttta acggccactt
    12541 tcctcaatac ccagttatgc caggtgttct gattatggaa gccttggcgc aaactgctgg
    12601 tgtgttggag ttatcaaaac ctgaaaataa aggaaaactg gtcttttacg ctggtatgga
    12661 caaggttaag ttcaagaagc aagttgtacc aggcgaccaa ttggttatga cagcgacttt
    12721 tgtaaaacgt cgtggcacca tagctgtggt tgaagcaaag gctgaagtgg atggcaagct
    12781 tgcagccagt ggtatcctta cttttgcaat tgggaactaa ggaggttctc catgtttcga
    12841 aaaattttaa ttgccaatcg tggtgaaatt gcggttcgta ttatccgtgc ggcacgtgaa
    12901 ttggggattg cgacggtagc ggtttattca actgctgata aggaagctct tcatacgctt
    12961 ttggcagatg aagcagtttg tattggtcct ggcaaggcaa cagagtctta tctcaatatt
    13021 aatgcagttc tatcagctgc agtcttgact gaggcagaag ctattcaccc tggttttgga
    13081 tttctcagtg aaaattccaa atttgcgacc atgtgtgaag aagtaggtat caagtttatc
    13141 ggtccatctg gtcatgttat ggatatgatg ggggataaga tcaatgcgcg tgctcagatg
    13201 attaaagcag gtgtgcctgt tataccaggt tcagatggag aagtgcataa ctctgaagaa
    13261 gctttgattg ttgctgaaaa aattggctat cctgttatgc tcaaggcttc agcaggtgga
    13321 ggtggtaaag ggattcgtaa ggttgaaaaa ccagatgacc tcgtttctgc ctttgaaact
    13381 gcctctagtg aggccaaggc caattatggc aatggtgcca tgtacataga acgggttatc
    13441 tatccagctc ggcacattga ggttcaaatc ctaggtgatg agcatggaca tgtgattcac
    13501 ttgggtgagc gggattgttc tcttcaaagg aataaccaaa aggttttgga agaaagtccc
    13561 tcgattgcaa tcggaaaaac gctgcgtcat gaaataggtg ctgctgctgt tcgagcggca
    13621 gagtttgttg gctatgagaa tgcaggaacc attgaatttc ttcttgatga agcaagtagc
    13681 aatttctatt tcatggagat gaatactcgt gttcaggtag aacatccagt aacagagttt
    13741 gtttcaggtg ttgatatcgt taaggaacag atttgcattg cggcaggtca gcctttgtct
    13801 gttaagcaag aagatattgt cctacgcggt catgccatcg agtgtcgtat caatgcagaa
    13861 aacccagcct ttaactttgc tccaagtcca ggtaagatta ctaatctcta tctgccaagt
    13921 ggtggagttg gcttgcgcgt ggattcagca gtttatccag gttataccat tccgccttat
    13981 tatgatagta tgattgccaa aatcatagta cacggcgaaa atcgttttga cgccttgatg
    14041 aaaatgcaac gtgccctcta tgaattagag attgaaggag tgcagaccaa tgcagatttc
    14101 cagcttgacc tcatttcaga tcgcaatgtc attgctgggg attatgatac ttccttcttg
    14161 atggaaacct tcttacctaa atatcaagaa aaagaataaa atgactttaa gagtttaaac
    14221 cgaaaagggg aatcaatggc tctatttagt aaaaaagata agtatattcg aatcaatccc
    14281 aatcgttcgg ttagggaaaa acctcaagct aagccagagg ttccagatga attattttcc
    14341 cagtgtccag gctgtaagca taccatctat cagaaggatc tgggaagtga acgtatctgt
    14401 ccgcactgta gctatacctt tcgtatttct gcccaagaac gcttggcttt gacgattgat
    14461 atgggaacct tcaaagaatt gtttacaggg attgaaagca aggatccctt gcatttccct
    14521 ggttaccaaa agaaactggc atctatgcgt gaaaaaacag gtctgcatga agccgttgtg
    14581 acaggaactg ctcttattaa aggtcagact gtggctcttg ggattatgga ttctaacttt
    14641 atcatggctt ctatgggtac ggttgtaggt gaaaaaatca ctcgtttgtt tgagtatgcg
    14701 actgtcgaaa aattgccagt tgtcctattc acagcctctg gtggagcccg tatgcaggaa
    14761 ggaatcatga gtctcatgca gatggctaag atctctgcgg cggttaaacg tcattcaaat
    14821 gctggtctct tttacctgac cattttgaca gatccaacga ctggtggtgt gacagcttct
    14881 ttcgctatgg aaggcgatat cattctggct gaaccacaga gcttggttgg ttttgctggg
    14941 cgtcgtgtga ttgaaaatac ggttcgtgaa agcttgcctg aggatttcca aaaggcagaa
    15001 ttcctattag aacatggctt tgtggatgct attgtcaaaa gaagagactt accagatacg
    15061 attgctagcc tagtcagatt gcatggaggg agtcctagat gaatattgca aaaatagtca
    15121 gagaagcgcg tgagcagagt cgcttgacaa ccttggactt tgcgacaggc atttttgatg
    15181 aatttatcca attacatggt gaccgttctt ttcgtgatga tggtgcagtt gttggtggta
    15241 ttggttggct tggagaccaa gctgtaacag ttgttggtat ccaaaaaggc aagagtttgc
    15301 aagacaacct caaacggaat tttggccaac cacatccaga aggctaccga aaggcactgc
    15361 ggttgatgaa acaggctgag aaatttggcc gtccagttgt gacctttatt aatacagcag
    15421 gtgcttatcc tggtgtcgga gcagaagaac gtggtcaagg ggaagctatc gctcgcaatc
    15481 tcatggaaat gagtgacctg aaagttccta ttatcgccat tattatcggt gaaggtggtt
    15541 caggcggggc tctggctcta gctgtcgcgg accgtgtctg gatgctggaa aattctatct
    15601 atgccattct cagtccagaa ggctttgctt ccattttatg gaaggacggt actcgcgcca
    15661 tggaagcagc agaactgatg aaaatcactt cgcatgaact gttagaaatg gacgtggtgg
    15721 ataaggtgat ttctgaagta ggactttcta gtaaagaact gattaagagt gtcaaaaaag
    15781 aactccaaac ggagctggct agactttcac aaaaaccgct agaagagttg ctggaagaac
    15841 gctatcaacg atttagaaaa tactaagaat gactaaatga gactagaaag gtctcatttt
    15901 ttatggaaaa atgcaattca ggttgaaaaa ttgacagttg aggagatggg ctataaaata
    15961 gacaaaaaag atgtatactg tagatgtaag cttacaaaaa t
//
LOCUS       DAVKGC010000038        15939 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKGC010000038 DAVKGC010000000
VERSION     DAVKGC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.038"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            98..1123
                     /gene="trpS"
                     /locus_tag="VW049_001020"
     CDS             98..1123
                     /gene="trpS"
                     /locus_tag="VW049_001020"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9432162.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1275..2753
                     /gene="guaB"
                     /locus_tag="VW049_001021"
     CDS             1275..2753
                     /gene="guaB"
                     /locus_tag="VW049_001021"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9432163.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2804..3901)
                     /gene="recF"
                     /locus_tag="VW049_001022"
     CDS             complement(2804..3901)
                     /gene="recF"
                     /locus_tag="VW049_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9432164.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSVQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNTTDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(3904..4272)
                     /gene="yaaA"
                     /locus_tag="VW049_001023"
     CDS             complement(3904..4272)
                     /gene="yaaA"
                     /locus_tag="VW049_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9432165.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4431..5681
                     /locus_tag="VW049_001024"
     CDS             4431..5681
                     /locus_tag="VW049_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9432166.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKGWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            5678..6961
                     /locus_tag="VW049_001025"
     CDS             5678..6961
                     /locus_tag="VW049_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9432167.1"
                     /translation="MTKVVFEKKYYPAVKEMIYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPAGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFSWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            7003..7824
                     /locus_tag="VW049_001026"
     CDS             7003..7824
                     /locus_tag="VW049_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9432168.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7835..8380
                     /gene="pgsA"
                     /locus_tag="VW049_001027"
     CDS             7835..8380
                     /gene="pgsA"
                     /locus_tag="VW049_001027"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9432169.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8377..9204
                     /locus_tag="VW049_001028"
     CDS             8377..9204
                     /locus_tag="VW049_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9432170.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLSENYLTESELEDKLWELL"
     gene            9189..10028
                     /locus_tag="VW049_001029"
     CDS             9189..10028
                     /locus_tag="VW049_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9432171.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10021..10815
                     /locus_tag="VW049_001030"
     CDS             10021..10815
                     /locus_tag="VW049_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9432172.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10874..11692
                     /gene="mreC"
                     /locus_tag="VW049_001031"
     CDS             10874..11692
                     /gene="mreC"
                     /locus_tag="VW049_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9432173.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11692..12186
                     /gene="mreD"
                     /locus_tag="VW049_001032"
     CDS             11692..12186
                     /gene="mreD"
                     /locus_tag="VW049_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9432174.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12280..13458
                     /locus_tag="VW049_001033"
     CDS             12280..13458
                     /locus_tag="VW049_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9432175.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13682..14461
                     /gene="rpsB"
                     /locus_tag="VW049_001034"
     CDS             13682..14461
                     /gene="rpsB"
                     /locus_tag="VW049_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9432176.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            14540..15580
                     /locus_tag="VW049_001035"
     CDS             14540..15580
                     /locus_tag="VW049_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9432177.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIKLLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            15826..>15939
                     /locus_tag="VW049_001036"
     CDS             15826..>15939
                     /locus_tag="VW049_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9432178.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELD"
BASE COUNT         5002 a         2520 c         3406 g         5011 t
ORIGIN      
        1 aaatctgtaa aaagcaaggc tgatttattt tgataaatta cggttatttc attaaaaaaa
       61 tgctataatt gaaaggacta tatcgaagga gaacaaaatg actaaaccca ttattttaac
      121 aggagaccgt ccaacaggaa aattgcatat tggacattat gttggaagtc tcaaaaatcg
      181 agtattatta caggaagagg ataagtatga tatgtttgtg ttcttggctg accaacaagc
      241 cttgacagat catgccaaag atcctcaaac cattgtagag tctatcggaa atgtggcttt
      301 ggattatctt gcagttggat tggatccaaa taagtcaact atttttattc aaagccagat
      361 tccagagttg gctgagttgt ctatgtatta tatgaatcta gtttcgttag cacgtttgga
      421 gcgaaatcca acagtcaaga cagagatttc tcagaaagga tttggagaaa gcattccgac
      481 aggattcttg gtctatccaa tcgctcaagc agctgatatc acagctttca aggctaatta
      541 tgttcctgtt gggacagatc agaaaccaat gattgagcaa actcgtgaaa ttgttcgttc
      601 ttttaacaat gcatataact gtgatgtctt ggtagagccg gaaggtattt atccagaaaa
      661 tgagagagca gggcgtttgc ctggtttaga tggaaatgct aaaatgtcta aatcactaaa
      721 taatggtatt tatttagctg atgatgcgga tactttgcgt aaaaaagtaa tgagtatgta
      781 tacagatcca gatcatatcc gcgttgagga tccaggtaag attgagggaa atatggtttt
      841 ccattatcta gatgtttttg gtcgtccaga agatgctcaa gaaattgctg atatgaaaga
      901 acgttatcaa cgaggtggtc ttggtgatgt gaagaccaag cgttatctac ttgaaatatt
      961 agaacgtgaa ctgggtccta ttcgtgagcg ccgtattgaa tttgctaagg atatgggaga
     1021 agtttataat atgattcaaa aaggtagtga aagagcgcgt gaagttgcgg gtcaaaccct
     1081 atctgaggta aaaggggcaa tgggacttca ttactttaac taagtttatt ttacaagaaa
     1141 ctatcttttc tatctcgaag aaaagatagt tttttattta cccctgtaac atttgacaaa
     1201 tttttataga atggtatgat agatacaata taaaaagagt caagctcaaa aagaaagaaa
     1261 agaggaaact tcgaatgtct aattgggaca ctaaattttt gaaaaaaggt tttacctttg
     1321 atgatgtatt gcttattcca gctgaaagtc atgtgttgcc taacgatgca gatttaacaa
     1381 ctaaattggc agataatttg actttaaata tcccaattat taccgctgcc atggacacag
     1441 ttacagagag tcaaatggcc attgctattg ctcgtgcagg cggtctcgga gttatccata
     1501 aaaacatgtc aattgctcaa caagcagacg aggttcgtaa ggtaaaacgt tctgaaaatg
     1561 gagttattat tgatccgttc ttcttgacgc ctgaacatac aattgctgaa gcagatgagc
     1621 ttatgggtcg ttaccgcatc agtggtgttc cagttgttga aacacttgaa aatcgtaaat
     1681 tggttggtat tttgacaaac cgagatcttc gttttatttc agattataat caaccaattt
     1741 caaaccatat gactagtgaa aatcttgtta ctgctcctgt gggtacggat cttgcaacgg
     1801 ctgagagtat tcttcaagag catcgtattg aaaaacttcc gttggtcgat gaagaaggca
     1861 gtctttctgg tttgatcact atcaaagata ttgaaaaagt tattgagttt ccaaatgcgg
     1921 ctaaagatga gtttggtcgt cttctagttg caggtgcagt aggtgttact tcagatacat
     1981 ttgaacgtgc agaggctctt tttgaggcag gagcggatgc gattgttatt gatactgcac
     2041 atggtcattc tgcaggtgtc ttgcgtaaaa ttgccgagat tcgtgctcat ttcccagatc
     2101 ggactttgat tgctggaaat attgctactg ctgaaggtgc acgtgccctt tatgaagcgg
     2161 gtgtagacgt tgttaaggtt ggtattggac caggttctat ctgtactact cgtgtgattg
     2221 ctggtgttgg tgttccgcaa gtaacagcta tctacgatgc tgcagctgtt gcgcgcgaat
     2281 atggtaaaac gattattgct gacggtggga tcaagtattc tggagatatt gtaaaagcac
     2341 ttgctgcagg tggaaatgct gttatgcttg gatctatgtt tgctggaact gatgaagctc
     2401 caggcgaaac tgaaatcttc caaggacgta aattcaagac ttaccgtggt atgggatcaa
     2461 ttgctgctat gaagaaaggt tcaagcgacc gttatttcca aggttctgtc aatgaagcaa
     2521 acaagcttgt tccagaagga attgaaggtc gtgttgctta taaaggagcg gcagctgata
     2581 ttgttttcca aatgattggt ggtattcgct ctggtatggg ttactgtggt gcagctaacc
     2641 ttaaagaact acacgataat gctcaattta ttgaaatgtc tggtgctggt ttgaaagaaa
     2701 gccatcctca tgatgtgcaa attactaatg aggcaccaaa ttattctatg taaaaaacaa
     2761 tgaaaagaac tccagtgaaa acaggagttc ttttacaatg ttgtcaattt ccatttacag
     2821 cagctttacc atcctgaata gtgaagatac ttagattttc tggcagattt tgaagatggt
     2881 ctaagcttgt tgttgtgata aaggtttgga ttgattgaga aatcgtttct aataatttta
     2941 actgtctagt gttgtcaagt tcactcatca catcgtcaag cagtaatatc ggagattctg
     3001 tggtaatgct ttccattaat tcgatttctg ctaattttat cgagaggacg agactacgat
     3061 gttgaccttg gcttccgaaa ctagcatcca tcccatttat ataaaaagaa atgtcatctc
     3121 gatgaggacc gacaccagta ttctttttaa ataaatctct ggatctactt ttttctaaag
     3181 caattttgaa agattcggat aagttttgtt tgtcagttgt attgacagaa gattgatagg
     3241 atattgacaa ctcttcgatc tgattagaga gttcaaaatg tttcttacgc ccaaatgatt
     3301 ctagtttttt tatgaaatct aagcggtgat tcattacacg acatccataa tcaactagct
     3361 gatcatctaa cacagaaagg aatgtttcat ctattttttg aactgatttt aggtaagtgt
     3421 ttctttgctt taggatgtgg ttataattgg ttaagtcaga taaatagatt ggcttaattt
     3481 gcccaagctc catatcaatg aattttcgtc gaatcgaagg tgctccttta attagttgta
     3541 aatcttcagg agcaaataag acaacattca tgtgtcctac ataatctgaa aggcgtgcct
     3601 gttttaagtg attaactttt gttacacggc ctttttgtgt tagttcgatt tctaggggaa
     3661 tggatccagt ttttttctga acgagacctg aaagatgaag ttgttcctca tcaaaatgaa
     3721 tgagattttt atctgttcga gttcgatgac tacgcgttaa ggctaaaaaa tagatagcct
     3781 ctaacatgtt tgtttttcct tgtgcattac gtcccaaaaa gacattcaat ttaggattaa
     3841 agtctatttt cgtttctttg taattacgaa aagtcttgag agatagatgt tgtagccaca
     3901 tgattatcta ccagggaatc gtggagcttg ttggctttta ggtgatgaag caggtttcga
     3961 tttgtctttt ttaactccct tattcatctt tttgacaagt ttagcgatcc gttctttttc
     4021 aactttatca gcttggtatt catcttgttc ttcagaagta ggttgtgtca acaagatgtc
     4081 aatgttcatg tcagggatgt caactttatc gccaatacga agtttttttc cacgacgact
     4141 ctctaattcc ctattaaagt aaacagaatg ttcagagaga aatgatttaa tagctcctcc
     4201 gctatgtgta attccaagtt ctttgagtag tgcttggagg gtaataaatt cttcaaataa
     4261 tttgtattcc ataattcacc tcaatctttg gtattatacc atattttcct aaaaatagtg
     4321 agagagatag ctagaaatgt aagcgatttt tatttcaaaa gtgttacagg gaacaagaaa
     4381 atttcaggtt ttcgtgatat aatagaagtc tgtatataag gaggtaaatc atggagttag
     4441 tgcatggaat ttcaacacat tttatccaat caaaaaagtt taaaacaaac aaaattaccg
     4501 tgcgttttac cgctccatta tcccttgata cgattgcagg tcacatgttg agtgcaagta
     4561 tgctagagac tgctaatcag atgtacccca cttctcaaga tttgaggaga cacttggcca
     4621 gtctatacgg tacagatatg tcaaccaatt gtttcagaag agggcaaagc cacattatag
     4681 aattgacatt tacctatgtt cgtgatgagt ttttaagtag gaaaaacgtg ctaacctctc
     4741 agattttgga acttgtaaaa gaaactcttt tttcacccgc agtagttgat aatgggtttg
     4801 atccggcctt atttgaaatt gagaaaaaac aattgctagc aagtttagca gctgatatgg
     4861 atgattcttt ttattttgca cataaagaat tggataaatt gttttttcat gatgaacgtc
     4921 ttcaattgga atatagtgat ttacgaaatc gtattttagc tgaaactcca caaagttctt
     4981 attcttgttt ccaagaattt ttagccaatg atcgaataga tttctttttc ctaggtgatt
     5041 ttaatgaggt tgaaattcaa aatgtattag aatcatttgg ctttaaaggt cgaaaaggag
     5101 atgtgaaggt tcagtattgt caaccttatt ctaatatcct tcaggaaggt atggttcgga
     5161 aaaatgtggg acaatccatt ttggaattag gttatcatta ccgttctaaa tatggtgatg
     5221 aacaacattt acccatgatt gtaatgaatg gtttacttgg tggatttgct cactctaagc
     5281 tctttacaaa tgtccgtgaa aatgctggat tagcttatac catttcaagt gagcttgatt
     5341 tatttagtgg attcttgagg atgtatgctg gtatcaatcg agaaaatcgt aaccaggctc
     5401 gtaaaatgat gaataatcaa ctgcttgatt taaaaaaagg ttattttaca gagtttgagt
     5461 taaatcagac caaggaaatg attcgttggt cgttgttact ttctcaagat aatcaatctt
     5521 cattgattga acgtgcttat caaaatgcct tatttggaaa atcttcagca gactttaaag
     5581 gttggattgc aaaactcgag caagttgaca aagatgctat ttgtagagca gctaataatg
     5641 tgaaactaca ggcgatttac tttatggaag gaatagaatg acaaaggttg tttttgaaaa
     5701 aaaatactat ccagctgtaa aagaaatgat ttatcgaact cgtttggcca acggattgac
     5761 agttgctctt ttgcctaaaa aggaatttaa agaggtttac gggagtgtca ctgtacagtt
     5821 tggttcggta gatacgtttg tcacagaagt tgacggagat gtaaaacaat atcctgcagg
     5881 aattgctcat tttcttgaac ataaattatt tgagagagaa gattctagtg atttgatgtc
     5941 ggcttttacg agtctaggtg cagatagtaa tgcctttaca agctttacaa aaacaaacta
     6001 tcttttttca gcaacggatt attttttaga aaatttatat ttacttgatg aattggtaac
     6061 atcagcacac tttactgaag cttccattct gacagagcag gatattattc agcaagaacg
     6121 agaaatgtac caagatgatc cagattcgtg tttattcttt tcaactttag cgaatttgta
     6181 tcctggtaca cctttagcaa ctgatatagt tggaagtgag gagtccattt cccaaatcaa
     6241 tctaactaat ttgcaagaaa attttacaaa gttttacaaa cctgtaaaca tgtctctgtt
     6301 tttagttggt aattttgatg tggagcgagt acaggactat tttgaaagca aagaactgaa
     6361 agattcagat tttcaggaag tagcaagaga aaagttgttt ttacagcctg taaagccaac
     6421 agatagtatg agaatggaag tatcttctcc caaactagcg attggagtta gaggtaagcg
     6481 agaagtttct gaagcggatt gctatcgaca tcatatttta ttaaaattat tgtttgcaat
     6541 gatgtttagt tggacttcgg atcgttttca aaaatgttat gaatcaggta aaattgatgc
     6601 atccttatct ctggaagttg aagtaacaag tcgctttcat tttgtcatgt tgacaatgga
     6661 tacgaaagag ccagttgctt tgtctcatca atttaggaag gctattcgta attttacaaa
     6721 ggatttagat attacagagg aacatttaga tattatcaaa agagagatgt ttggcgaatt
     6781 tttcagtagc atgaactctc ttgaatttat tgcaacgcaa tatgatgctt ttgaaaatgg
     6841 tgagacaatt tttgatttgc cgaaaatttt acaggaaatt actttagagg atgtccttga
     6901 tgctggacat catttaatag atgatggtga catagttgat tttacaatat tcccatcgta
     6961 gtaacctatt ataatagaca ctagaaagaa gggatgacaa gtatgagaaa aaaaacaatt
     7021 ggagaggttt tacgattagc tagaatcaat cagggattga gtttagatga attgcagaaa
     7081 aagacagaaa tccagttaga tatgttggaa gcaatggaag cagacgattt cgatcaactt
     7141 ccaagtcctt tttacacgcg ttctttcttg aaaaaatatg catgggctgt tgagttagat
     7201 gaccaaattg ttttggatgc ttatgattct gggagtatga ttacttatga ggaagtagat
     7261 gttgatgaag atgagttgac aggtcgtaga cgttcaagta agaaaaagaa gaaaaaaaca
     7321 tcatttttac ctttatttta ttttatcctt tttgctttat cgattttaat ttttgtgact
     7381 tattatgttt ggaactatat tcaaactcaa ccagaggagc cttctctttc taattacagt
     7441 gtggttcaat caacaagttc aactagctct gttccccact cctcaagtag tagttcttct
     7501 agtatagaat cagctataag tgtatcaggc gaaggaaatc atgtagaaat cgcttataag
     7561 acaagtaagg aaacagttaa attgcaattg gcagtttcag atgttacaag ttgggtcagt
     7621 gtttcagaaa gcgaacttga gggcggtgta accttatcgc caaagaagaa aagtgcagaa
     7681 gcaacagttg caactaaaag tcctgtaaca attacgttag gtgttgtaaa aggtgttgat
     7741 ttgacagtag ataatcagac tgttgattta tcgaaattaa cagctcagac tggacaaatc
     7801 actgtaacct ttactaaaaa ttaaggaaaa acgaatgaaa aaagaacaaa ttcccaatct
     7861 cttaacaata ggtcgaattc tctttatacc tatttttatc tttattttaa cgataggaaa
     7921 ttcgatagag agtcatatag ttgcagctat tatctttgct gttgccagta ttaccgacta
     7981 tttagatgga tatttagctc gtaaatggaa tgtggtcagt aattttggta aatttgcaga
     8041 tcctatggcg gataagttac tagttatgtc ggcttttatt atgttgattg agttaggtat
     8101 ggctccggct tggattgttg cagtgattat ctgtcgtgag ttagctgtga caggtttaag
     8161 gcttttattg gttgaaactg gtggaacaat tttagcagca gcaatgcctg gaaaaattaa
     8221 aacttttagt cagatgtttg ccattatttt cttgctatta cattggactt tgcttggtca
     8281 agttctactt tatgtagcct tatttttcac tatctactct ggctatgact atttcaaggg
     8341 tagtgcctat gtatttaaag ggacatttgg ttcgaaatga aatcaataat tgatgtaaaa
     8401 aatctttctt ttcgctataa agaaaatcag aactactacg atgtgaagga tattacgttt
     8461 cacgtgaaac gtggagaatg gctttcgatt gtagggcata atggtagtgg taaatcaacg
     8521 acggttcgat taattgatgg cttactggaa gcagaatccg gagagattgt aattgatggc
     8581 caacgtttga ctgaggaaaa tgtttggaat atacgtcgtc aaatcggtat ggtttttcaa
     8641 aatccagaca atcaatttgt tggagcgact gttgaagatg atgttgcttt tggtttggaa
     8701 aatcagggac tttctcgtca agaaatgaaa aagagagtgg aagaagctct ggctttagtt
     8761 ggcatgttgg actttaaaaa gagagagcca gcgcgtctat caggtggtca aaagcaacgt
     8821 gtggccattg caggtgttgt agccctaaga ccagctattt taatcttaga tgaagcaacg
     8881 agtatgttgg atcctgaggg gcgtagagaa cttattggga cagtaaaagg aattcgaaaa
     8941 gactatgata tgacggtcat ttctattacc catgatttgg aagaagtcgc catgagtgat
     9001 cgcgtattgg tcatgaaaaa aggggaaatt gaatcaacta gtagtccaag ggagcttttc
     9061 tctcgaaatg atttagatca aattggatta gacgatcctt ttgctaatca attaaaaaaa
     9121 tctttgagcc agaatggcta tgatttatct gaaaattatt tgacagaaag tgagctagag
     9181 gataagctat gggaattgct ctagaaaatg tgaattttac atatcaagaa ggtactccct
     9241 tagcttcagc agctttgtcg gatgtttctt tgacgattga agatggctct tatacagctt
     9301 taattgggca cacaggtagt ggtaaatcaa ctattttaca actcttaaat ggtttattgg
     9361 tgccaagtca agggagtgtg agggtttttg ataccttaat cacctcgact tctaaaaata
     9421 aagatattcg tcaaattaga aaacaggttg gcttggtatt tcagtttgct gaaaatcaga
     9481 tttttgaaga aacggttttg aaggacgttg cttttggacc gcaaaatttt ggagtttctg
     9541 aagaagatgc tgtgaagact gcgcgtgaga aactggctct ggttggaatt gatgaatcac
     9601 tttttgatcg tagtccgttt gagctgtcag ggggacaaat gagacgtgtt gccattgcag
     9661 gcatacttgc catggagcca gctatattag tcttagatga gccaacagct ggtctagatc
     9721 ctctagggag aaaagagttg atgaccctgt tcaaaaaact ccaccagtca gggatgacca
     9781 tcgtcttggt aacgcatttg atggatgatg ttgctgaata tgcgaatcaa gtctatgtaa
     9841 tggaaaaggg acgtttagta aaggggggca aaccaagtga tgtctttcaa gacgttgttt
     9901 ttatggaaga agttcagttg ggagtaccta aaattacggc cttttgtaaa cgattggctg
     9961 atagaggcgt gtcatttaaa cgattaccga ttaagataga ggagttcaag gagtcgctaa
    10021 atggatagta tgattttggg gcgttatatc ccaggggatt cgattgttca ccgattggat
    10081 ccacgtagca aattgcttgc tatgatgcta ctgattttga ttgtgttttg ggctaataac
    10141 cccttgacga atctgattct ttttatagcg acagggatat ttattgcctt gtcaggagta
    10201 tctctttcat tttttattca gggcttgaag tctatgtttt tcttgattgc cttcacaact
    10261 atttttcaac tatttttcat ttctaatggg aatgttttat ttgagttttc gtttgtgaga
    10321 atcacggatt atgctttgca acaagctggg attatttttt gtcgctttgt attgattatt
    10381 ttcttttcaa ctttgttaac cttaacaacc atgcctttaa gtttggcatc agctgtcgaa
    10441 gctttattag cacctttaaa gcgtgtgaaa gttccagttc atgaaattgg attgatgctg
    10501 tctatgagtt tgcgttttgt cccaaccttg atggatgata cgacgcggat tatgaatgca
    10561 cagaaagctc gtggagtgga ttttggagaa ggaagcatcg ttcaaaaagt aaaggcgatg
    10621 attcccattt tgattcctct ttttgcgaca agtttaaaac gtgcagattc cttggctatc
    10681 gctatggaag cgcgtggcta tcagggtgga aaaggcagaa gtcaataccg acaattgaaa
    10741 tggactctaa aggatacgtt gaccattctt gttattctcg tacttggttg ttgtttattt
    10801 ttcttaaaat cttagtagct ttcaggaatt gataaaaagt tactgtaaca gtttttgata
    10861 taaaggtagg aatatgaacc gttttaaaaa atcaaaatat gtcattattg tttttgtcac
    10921 tgttctgctt gtgtcagctc tcttagcgac gacttattca agtacaattg tgacaaaatt
    10981 aggagatgga atctcattgg ttgatagagt tgtacaaaaa ccttttcagt ggtttgattc
    11041 tgtcaaatca gatttggctc atttgacacg aacatataat gaaaatgaaa gtttgaagaa
    11101 acagctttac caattagaag ttaaatcaaa tgaggtggaa agtttaaaga cagaaaatga
    11161 acaactgcgc caattgcttg atatgaagtc taaattgcaa gccacaaaga ctttagcagc
    11221 agatgttatt atgcgttctc cggtatcttg gaagcaggag ttgaccttag atgcaggtag
    11281 atcaaaaggt gcttctgaga acatgttagc tattgcaaat ggtggcttga ttgggagtgt
    11341 ttcaaaagta gaggagaact ctactatagt caaccttctg acaaatacgg aaaatgctga
    11401 taagatttct gttaaaattc aacatggctc tactacaatt tatggaatta ttattggcta
    11461 tgacaaggaa aatgacgttc ttaaaattag ccaattaaat agtaatagcg atattagtgc
    11521 gggagataag gtgactactg gtggattagg aaactttaac gttgctgata ttcctgttgg
    11581 tgaagtggtt gccacaacgc atagtacaga ctatttgaca cgagaagtaa ctgttaaatt
    11641 gagtgcagat actcataatg tagatgtgat agaattagtg gggaattcat aatgagacag
    11701 ttgaagcgag ttggagtatt tttattgctt cctttctttg ttctaattga cgcccatatt
    11761 agccagcttc tgggctcatt tttcccccat gtacatttgg ctagtcattt tctttttcta
    11821 tttctcttat ttgagacgat agaagtatca gagtatctct acctagtcta ttgttttgtt
    11881 ataggcttgg tttatgatgt ttactttttc catctaatag ggattacaac tctcttattt
    11941 atcttattgg gagccttcct tcataaattg aatagtgtta ttttgttgaa tcgttggaca
    12001 agaatgctag ctatgattgt gctgacattc ctgtttgaaa tgggtagtta tcttttggct
    12061 tttatggtag ggttgacagt agatagcatg tcgattttta tagtctatag cttggtaccg
    12121 acgatgattt taaatttttt atggattact gtttttcaat ttatttttga aaaatattat
    12181 ctataagaac gacatataaa tgtaacaaag gcgtaatatt tattaggcct ttttttggta
    12241 tactagtatt gtctttaaaa gaaggagtat ctacgtaata tgaagaaaaa aatcttagcg
    12301 tcacttttat taagtacagt aatggtttct caagtagctg ttttaacaac tgcgcatgca
    12361 gaaacgactg atgacaaaat tgctgctcaa gataataaaa ttagtaactt aacagcacaa
    12421 caacaagaag cccaaaaaca agttgaccaa attcaggagc aagtatcagc tattcaagct
    12481 gagcagtcta acttgcaagc tgaaaatgat agattacaag cagaatctaa gaaactcgag
    12541 ggtgagatta cagaactttc taaaaacatt gtttctcgta accaatcgtt ggaaaaacaa
    12601 gctcgtagtg ctcaaacaaa tggagccgta actagctata tcaataccat tgtaaactca
    12661 aaatcaatta cagaagctat ttcacgtgtt gctgcaatga gtgaaatcgt atctgcaaac
    12721 aacaaaatgt tagaacaaca aaaggcagat aaaaaagcta tttctgaaaa acaagtagca
    12781 aataatgatg ctatcaatac tgtaattgct aatcaacaaa aattggctga tgatgctcaa
    12841 gcattgacta cgaaacaggc agaactaaaa gctgctgaat taagtcttgc tgctgagaaa
    12901 gcgacagctg aaggggaaaa agcaagtcta ttagagcaaa aagcagcagc tgaggcagag
    12961 gctcgtgcag ctgcggtagc agaagcagct tataaagaaa aacgagctag ccaacaacaa
    13021 tcagtacttg cttcagcaaa cactaactta acagctcaag tgcaagcagt atctgaatct
    13081 gcagcagcac ctgtccgtgc aaaagttcgt ccaacataca gtacaaacgc ttcaagttat
    13141 ccaattggag aatgtacatg gggagtaaaa acattggcac cttgggctgg agactactgg
    13201 ggtaatggag cacagtgggc tacaagtgca gcagcagcag gtttccgtac aggttcaaca
    13261 cctcaagttg gagcaattgc atgttggaat gatggtggat atggtcacgt agcggttgtt
    13321 acagctgttg aatcaacaac acgtatccaa gtatcagaat caaattatgc aggtaatcgt
    13381 acaattggaa atcaccgtgg atggttcaat ccaacaacaa cttctgaagg ttttgttaca
    13441 tatatttatg cagattaatt tacagaggga ctcgaataga gccctctttt caggttttac
    13501 cgtgacaatc cctattaaaa attatatcaa aatagcttga aaatattgga aaagtatggt
    13561 aaaatgaaaa ttgtcgtgtg aacgataata ctcattcttg atgaattgtg aagcagttgc
    13621 ccttgggtcg ttttgcgagt tgaagtcaag aagaggaaaa aaacaaaaag gagaaatact
    13681 catggcagta atttcaatga aacaacttct tgaggctggt gtacactttg gtcaccaaac
    13741 tcgtcgctgg aatcctaaga tggctaagta catctttact gaacgtaacg gaatccacgt
    13801 tatcgacttg caacaaactg taaaatacgc tgaccaagca tacgacttca tgcgtgatgc
    13861 agcagctaac gatgcagttg tattgttcgt tggtactaag aaacaagcag ctgatgcagt
    13921 tgctgaagaa gcagtacgtt caggtcaata cttcatcaac caccgttggt tgggtggaac
    13981 tcttacaaac tggggaacaa tccaaaaacg tatcgctcgt ttgaaagaaa ttaaacgtat
    14041 ggaagaagat ggaactttcg aagttcttcc taagaaagaa gttgcacttc ttaacaaaca
    14101 acgtgcgcgt cttgaaaaat tcttgggtgg tatcgaagat atgcctcgta tcccagatgt
    14161 gatgtacgta gttgacccac ataaagagca aatcgctgtt aaagaagcta aaaaattggg
    14221 aatcccagtt gtagcgatgg ttgacaccaa tactgatcca gatgatatcg atgtaatcat
    14281 cccagctaac gatgacgcta tccgtgctgt taaattgatc acagctaaat tggctgacgc
    14341 tattatcgaa ggacgtcaag gtgaggatgc agtagcagtt gaagcagaat ttgcagcttc
    14401 agaaactcaa gcagattcaa ttgaagaaat cgttgaagtt gtagaaggtg acaacgctta
    14461 atttatacaa atagtaatta cctaggaggg cggggcttag cccggctctc ctattttcaa
    14521 aaaatatagg agaattaaaa tggcagaaat tacagctaaa cttgtaaaag agttgcgtga
    14581 aaaatctggt gccggtgtta tggacgctaa aaaagcgctt gtagaaacag acggtgacat
    14641 cgaaaaagcg attaaattgc ttcgtgaaaa aggtatggct aaggcagcta agaaagctga
    14701 ccgtgttgct gcagaaggtt tgactggtgt ttatgttaac ggtaatgttg cagcagttat
    14761 tgaagtaaac gctgaaactg acttcgttgc aaaaaacgct caattcgttg aattggtaaa
    14821 tactacagct aaagtcattg ctgaaggaaa acctgctaat aatgaagaag ctcttgcttt
    14881 gataatgcct tcaggtgaaa ctcttgaagc tgcatacgta tctgcaacag caactatcgg
    14941 agagaaaatc tcattccgtc gctttgcatt gattgaaaaa acagacgcac aacactttgg
    15001 agcataccaa cataacggtg gacgtatcgg tgttatttca gttgttgaag gtggagacga
    15061 agcacttgct aaacaattgt caatgcacat cgcagcgatg aaaccaacag ttctttctta
    15121 caaagaattg gatgagcaat tcgttaaaga tgagttggca caattgaatc acgttatcga
    15181 ccaagacaac gaaagccgtg caatggttaa taaaccagct cttccacact tgaagtatgg
    15241 atcaaaagct caattaactg atgatgttat tgctcaagct gaagctgaca tcaaagctga
    15301 attggctgca gaaggcaaac cagaaaaaat ctgggacaaa attatcccag gtaaaatgga
    15361 tcgcttcatg cttgataata ctaaagttga ccaagcttac acacttcttg cacaagttta
    15421 catcatggat gacagcaaga cagttgaagc ataccttgaa tcagttaacg cttcggtagt
    15481 tgagtttgct cgctttgaag ttggtgaagg tatcgagaaa gctgcaaacg acttcgaagc
    15541 tgaagttgca gctacaatgg cagcagcctt gaataactaa taatagaaag gaagcaattt
    15601 gcttcctttt ttgtataggg cttatatttt tcctgaaatt gtttttcccc cagtctcact
    15661 cgattagaat aattaaaaca aaaagcccta taataagggc taagtgcatt taggagacta
    15721 tacttcaaat tcatagtaag cgcgtcataa caaggtatct atcctaattc cccaacttcc
    15781 agtatactag ataaaaaata attatcggag gaaaggtatt gtactatgat gattcaacta
    15841 agtgatttag gtcaagttca cattgtttgt ggcaagacag atatgaggca gggaatagac
    15901 tcattagcct atgtagttaa aacccacttt gaattggat
//
LOCUS       DAVKGC010000039        15595 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKGC010000039 DAVKGC010000000
VERSION     DAVKGC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.039"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            245..2032
                     /locus_tag="VW049_001037"
     CDS             245..2032
                     /locus_tag="VW049_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9432179.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2029..2505
                     /locus_tag="VW049_001038"
     CDS             2029..2505
                     /locus_tag="VW049_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9432180.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2533..3438
                     /locus_tag="VW049_001039"
     CDS             2533..3438
                     /locus_tag="VW049_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9432181.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3425..4240
                     /locus_tag="VW049_001040"
     CDS             3425..4240
                     /locus_tag="VW049_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9432182.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4247..4651
                     /locus_tag="VW049_001041"
     CDS             4247..4651
                     /locus_tag="VW049_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9432183.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4904..6070
                     /locus_tag="VW049_001042"
     CDS             4904..6070
                     /locus_tag="VW049_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9432184.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6186..7223
                     /locus_tag="VW049_001043"
     CDS             6186..7223
                     /locus_tag="VW049_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9432185.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7431..8285
                     /locus_tag="VW049_001044"
     CDS             7431..8285
                     /locus_tag="VW049_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9432186.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAG"
     gene            8435..8692
                     /locus_tag="VW049_001045"
     CDS             8435..8692
                     /locus_tag="VW049_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU9432187.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(9112..9204)
                     /locus_tag="VW049_001046"
                     /pseudo
     CDS             complement(9112..9204)
                     /locus_tag="VW049_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9240..9536)
                     /locus_tag="VW049_001047"
     CDS             complement(9240..9536)
                     /locus_tag="VW049_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9432188.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(9526..9687)
                     /locus_tag="VW049_001048"
     CDS             complement(9526..9687)
                     /locus_tag="VW049_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU9432189.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            9757..10521
                     /locus_tag="VW049_001049"
     CDS             9757..10521
                     /locus_tag="VW049_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9432190.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRYWGSEAFNKALELSLASVHHL"
     gene            complement(10761..10913)
                     /locus_tag="VW049_001050"
     CDS             complement(10761..10913)
                     /locus_tag="VW049_001050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432191.1"
                     /translation="MRTKASLRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            11131..12510
                     /locus_tag="VW049_001051"
     CDS             11131..12510
                     /locus_tag="VW049_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9432192.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            12524..13189
                     /locus_tag="VW049_001052"
     CDS             12524..13189
                     /locus_tag="VW049_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9432193.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            13499..14038
                     /locus_tag="VW049_001053"
     CDS             13499..14038
                     /locus_tag="VW049_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9432194.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            14105..14515
                     /locus_tag="VW049_001054"
     CDS             14105..14515
                     /locus_tag="VW049_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9432195.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIELTTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            14631..>15595
                     /gene="pavB"
                     /locus_tag="VW049_001055"
     CDS             14631..>15595
                     /gene="pavB"
                     /locus_tag="VW049_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9432196.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKLEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPVAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLE"
BASE COUNT         4360 a         2959 c         3546 g         4730 t
ORIGIN      
        1 gcgatatagg gattgctcat attttatcaa tgtattctat acaaaattat tttaggtcct
       61 atattccgaa tttaaagagc gtttgaaatg ggtctaaaaa tgtttgaaac taattgaatg
      121 tgtgtataat agtaaaaatg cagtaaatat cccgacaatt tctattgaca attcaaacag
      181 attggtttat aattaatata acaacaaatg aaatcgcaaa ctttcgcggt cggaaggtag
      241 ttttatgaca cgatttgaga tacgagatga tttctatctc gatggaaaat catttaagat
      301 tttatctggt gccattcatt attttagggt tcctccagag gattggtatc attcgctcta
      361 taacttgaag gctcttggtt ttaatacggt agagacttat gttgcttgga atttacacga
      421 gcctcgtgaa ggtgagtttc attttgaagg tgatctggat ttagagaaat ttctccaaat
      481 agcgcaggat ttgggtctct acgcaattgt gcgtccgtct ccatttatct gtgcggaatg
      541 ggaattcggt ggcttaccag cttggctctt gaccaagaac atgcgaattc gctcatccga
      601 cccagcatat atcgaggcag ttggtcgcta ctatgatcag ttattgccaa gactggtgcc
      661 tcgtttgttg gacaatggtg gcaatattct catgatgcag gttgaaaatg agtatggttc
      721 ttacggagaa gataaggctt acctgagagc gattcgacag ctaatggaag agtgtggcgt
      781 aacctgtccc ctctttacat cagatggtcc atggcgagct actctgaaag ctggaacctt
      841 aattgaagag gacctctttg taacaggaaa ctttggttct aaggcacctt acaacttttc
      901 gcagatgcag gaattctttg atgaacatgg taagaaatgg ccactcatgt gtatggagtt
      961 ctgggatggt tggttcaatc gctggaaaga accgattatc acacgggatc ctaaggaatt
     1021 ggcagatgca gttcgagagg ttttggaaca aggctctatc aatctttaca tgttccacgg
     1081 tggtacaaac tttggtttca tgaatggttg ctcagctcga ggaactttgg acctgccaca
     1141 agttacgtct tatgattacg atgcccttct ggatgaagaa ggaaatccaa ctgctaaata
     1201 tcttgcagtc aagaagatga tggcaacaca tttttcagag tatccgcagt tggaaccact
     1261 ctacaaagag agtatggagt tggatgctat tccactagtt gaaaaagttt ctttgtttga
     1321 aaccttagat agcttgtcaa gtcctgtaga aagtctctat cctcaaaaga tggaggagct
     1381 gggacaaagt tatggctacc tactttatcg aacagaaaca aactgggatg cagaagaaga
     1441 aagacttcgt atcattgatg gtcgagatag ggcccagctg tatgtcgatg gtcagtgggt
     1501 taaaactcaa tatcagacag agattgggga agatattttt tatcaaggta aaaagaaagg
     1561 gctatctagg ttagatatct tgatagaaaa tatggggcgt gtcaactatg ggcataagtt
     1621 cttagcggat acgcaacgta agggaattcg gacaggggtc tgtaaggatc tgcatttctt
     1681 actaaactgg aaacactatc cactcccact agacaatcct gagaaaattg atttttcaaa
     1741 aggatggact caaggacaac cagcctttta cgcttatgac tttacagtcg aagagccaaa
     1801 agatacttac ctagacttgt ctgagtttgg taagggggtt gcctttgtca atgggcagaa
     1861 tctaggacgt ttttggaacg ttggcccaac tctctcactt tatatccctc atagctatct
     1921 caaggaaggt gccaaccgca tcattatctt tgaaacagaa ggtcaatata aagaagagat
     1981 tcatttaact cgtaaaccta cactaaaaca tataaagggg gaaaacttat gacaattgta
     2041 ggatgccgta ttgatggacg tttgatccac ggacaagtag ccaatctttg ggctggaaaa
     2101 ctaaatgttt cacgcattat ggttgtagac gacgaagttg tcaacaacga tattgaaaag
     2161 agtggtttga aacttgcgac accaccaggt gtgaaattga gtattttgcc agttgagaaa
     2221 gctgcagcca atattcttgc tggcaaatac gatagccaac gtctctttat cgtggctcgt
     2281 aaaccagacc gcttccttgg tttggtagaa gcaggtgtac cacttgaaac ccttaatgtt
     2341 gggaatatgt ctcaaacacc agaaactcgt tctattacac gttctatcaa cgtagtagac
     2401 aaggatgtgg aagacttcca caaactggca gaaaaaggtg ttaaacttac tgctcagatg
     2461 gttccaaatg atccaatttc agactttttg agcttattaa aataggaaaa aattttttag
     2521 gaggtcattg ttatgataca atggtggcaa attttacttc tcactttgta ctcagcttat
     2581 caaatctgtg atgagttgac gatcgtttca tctgcaggtt cccctgtatt tgctggtttc
     2641 attactggtt taatcatggg agatgtgact actggtttac ttatcggtgg taacttgcaa
     2701 ctgttcgttc ttggggttgg taccttcggt ggtgcttctc gtatcgacgc aacttctggt
     2761 gcggttcttg cgacagcctt ctctgtttca caaggaattg atgcaccgct tgcaattact
     2821 acaatcgctg taccagtagc agctctcttg acttacttcg acgttcttgg tcgtatgact
     2881 actaccttct tcgctcaccg tgtggatgct gcaatcgaac gctttgacta taaaggtatt
     2941 gaacgcaact acttgcttgg tgcgattccg tgggctctat ctcgtgccct tccagtcttc
     3001 tttgcccttg cttttggtgg tgcctttgta caatcagtag tagacttcgt tgaagcctac
     3061 aaatgggttg cagatggctt gacacttgca ggacgtatgc ttccaggtct tggatttgca
     3121 atcttgcttc gttaccttcc agttaaacgt aaccttcact accttgctat gggatttggt
     3181 ttgacagcta tgttgactgt tctttactca tatgtaacag gtcttggtgg cgctgttgct
     3241 ggtatcgtag gtactcttcc tgctgaagtt gctgaaaaaa ttggtttcgt gaacaacttc
     3301 aaaggtttgt ctatgattgg tatttctatc gtaggtattt tccttgcagt gcttcacttc
     3361 aaaaatagcc aaaaagtagc tgtagcagca ccttctacac catcagaaag tggggaaatc
     3421 gaagatgacg aattctaatt acaaacttac aaaagaagat tttaatcaaa tcaacaaacg
     3481 tagcttgttt actttccaat taggttggaa ctacgaacgt atgcaagctt ctggttacct
     3541 ttacatgatc ttgcctcagt tgcgtaaaat gtatggtgat ggaactcctg aattgaaaga
     3601 aatgatgaaa gttcatactc aattcttcaa tacttcacca ttcttccata ccattatcgc
     3661 tggttttgac cttgccatgg aagaaaaaga tggtgtaggt tcaaaagacg ctgttaacgg
     3721 tatcaagaca ggtttgatgg gaccattcgc tcctcttggg gatacaatct ttggttcact
     3781 tgtacctgct atcatggggt cagtcgcagc aactatggct atcgctggcc aaccttgggg
     3841 gatcttcctt tggattgcag ttgcagtagc gtatgacatc ttccgttgga aacagttgga
     3901 atttgcttac aaagaagggg ttaaccttat caacaacatg caaagtacct tgacagcttt
     3961 gattgacgct gcatctgtac ttggtgtctt catgatgggt gctcttgtag caacagtgat
     4021 taactttgaa atttcttaca agttgccaat cggtgaaaag atgattgatt tccaagacat
     4081 cttgaaccaa atcttcccac gtttgcttcc agcaatcttt actgccttta tcttctggtt
     4141 gcttggtaaa aaaggtatga actctactaa agctatcggt attattatcg tacttgcttt
     4201 ggctctttct gcccttggtc actttgcact tggaatgtaa ttccttatga ctaaatcatt
     4261 aattttggtg agccatggtc gcttctgtga ggagcttaga ggtagcacag aaatgattat
     4321 gggcccacaa gacaacattt acacagtagc tcttcttcca gaagatggcc cagaagaatt
     4381 tactgctaaa tttgaagctg ttattgaagg attggatgat ttcctagtct ttgcggatct
     4441 tctcggtggg acaccttgta atgtggtgag tcgcttgatc atggaaggtc gtgatattga
     4501 cctttacgca gggatgaatc ttccaatggt gattgaattt atcaatgcga gccttacagg
     4561 cgcagatgcg gactacaaga gccgtgctgc agaaagcatt gtgaaagtta atgacctgtt
     4621 agcgggcttc gatgatgacg aagatgaata atactcttcg aaaatctctt caaactacgt
     4681 caacatcgcc ttgccgtagg tatatgttac tgacttcgtc agtcttatcc ggcaacctca
     4741 aagcagtgct ttgagcagcc tgcggctagt ttcctacaga ttttagttgg aactcgattc
     4801 aattcatgtg acaacgtgaa aatcgttaga gcattttata tagaatatac atgggaatgg
     4861 agcttactcc cattcccata tttaatagaa aaagaggaac tcaatgctac attatacaaa
     4921 agaagacttg ctcgaattgg gtgcagaaat cactacgcgt gaaatctacc aacagcctga
     4981 tgtatggaga gaagcttttg aattttatca agcaaaacgt gaagaaattg cagcctttct
     5041 acaagaaatc gctgataaac atgactatat taaggttatc ttgacaggtg ctgggacttc
     5101 tgcttatgtg ggagatacct tgctacctta ctttaaggaa gtctatgacg aacgcaaatg
     5161 gaatttcaat gctattgcga caacagatat cgttgccaaa ccagcaacct atttgaaaaa
     5221 agatgtggca actgtccttg tgtcttttgc tcgtagtggg aactcgcctg aaagtgtggc
     5281 gactgttgat ttggccaaat ccttggtgga tgagctttat caagtgacga ttacttgtgc
     5341 agcagatggt aaattggctc ttcaagctca cggtgatgac cgtaatctct tgctcttgca
     5401 accagctgtc tctaatgatg ctggatttgc catgacttct agctttacgt ctatgatgtt
     5461 gacagctctc ttggtctttg atcctacaga atttgctgtt aagtctgaac gttttgaagt
     5521 tgtatctagt cttgcccgta aagttttaga caaggtagaa gatgtcaaag agctcgttga
     5581 tttagacttt aaccgtgtca tctatctagg cgctggtcct ttctttggac ttgctcatga
     5641 agctcagctc aagattttgg aattaactgc tggtcaagtt gcgaccatgt atgaaagccc
     5701 agttggcttc cgtcacggtc caaaatctct tatcaacgac aatacagttg ttttggtctt
     5761 tggtacaacg acagcctaca ctcgtaagta cgacttggac ttggttcgtg aagttgctgg
     5821 tgaccagatt gctcgtcgtg ttgtgctttt gagtgatcaa gcttttggtc ttgaaaatgt
     5881 caaagaagtg gcccttggtt gtggcggtgt cttgaatgat atttaccgtg tcttccctta
     5941 catcgtttat gcccaactct ttgccctatt gacttcactc aaggtagaaa ataaaccaga
     6001 tacaccgtct cctacaggta cagtaaaccg tgtagtacaa ggtgtcataa ttcacgaata
     6061 tcaaaagtaa gacagtgttt atgaattctt gacaagagga tttgtaaatt atcagataaa
     6121 ccatagattg tcagtacgct ttctatggtt tgtttgcttg agagaaatag taaaaggaga
     6181 acagaatgaa agcatacaca gagcgtgtat ttggaaatgt tgagggcgag gatgtcttgg
     6241 cctatcgatt tgagacagac ggtggctacc aacttgaggt tatgacttat ggcgcgacca
     6301 tcttgcgcta tgtcgcacct gacaaggctg gaaattttgc caatgttatc ttgggatttg
     6361 atgactttga tagttatgta ggcaatagtc ccaagcatgg agcaagtgta ggtcctgtag
     6421 cgggtcgtat tgcaggtgcg acctttgagc tcaatggtaa gacctatgac cttgaggtta
     6481 ataatgctag caactgtaat cacagtggtt caactggttg ggattccagc ttgtttgaag
     6541 ttgaagaagt aagcgatcat ggcttgactc tctacacaga gcgtacagat gggacaggag
     6601 ggttccctgg aaatctcaag atttggatca gttatcactt ggaagaaact ggtgcctatg
     6661 aaatcatcta caaggtaacg accgatcagg atacgctggt caatccaacc aaccacagct
     6721 atttcaactt gtctggtgat ttcacgcaga cgattgaccg tcatgtcttc caactaaaca
     6781 cagagggcat ttactcaatc gctcctgacg gtgttcctgc caaaactcca gaagccaacc
     6841 gtgatgtggt caaacacatc tacaatggta ccttgttgaa ggatatcttt gcagaagaag
     6901 atgagcaaat ccagctggca tcaggtttgg atcacccatt tgcccttcct gcaggccatg
     6961 acaatgctgg attcctttat gaccaaaatt caggtcgctt cctgcttttc aagacagaag
     7021 ctccttgctt tgtggtctac acagcaaact ttgtggatga aagtgtcatc ataggaggtc
     7081 agccaatgct acagcacaat gggattgctc ttgaagcgca agctttacca gatgccattc
     7141 acagtgacct taaaggccaa gtcattctta aagctggtca aaccttcacc agtaagacac
     7201 gttatgaact tgttgtgaaa taaaaaaagc tggtcaaaaa gtctttaaaa ttaaaataac
     7261 gcatattgtc aggtgatgaa aagtttgata atatgcgttt tattatgaaa tgatttactt
     7321 tatcttatcc tgaaatggtg ttatttttga gttatctggc ttgactttct tgatggaaat
     7381 tatataatag ttgtaataac aataattaca acgagaaaag aggaccattt atgacctatg
     7441 aatacaagag tcacatttat ttggcagaga cagctttaaa tgtaaaggat ttggccagtc
     7501 aaacagcctt ttatcagcaa gttattggtt tagaaatctt atctcaaact gagacagagt
     7561 ccattctagg ccttggtgga aaagtcttgg tacagctgat tcaagcacaa gagagcggag
     7621 aagtgaggga acattatggt ctttaccatt tggccattct cttgcccaca cgcaaggctt
     7681 tggctgatgt cttgaaacac ctgacagatt tacaaattcc tcttgtcggc ggtgcagacc
     7741 atggttacag tgaagctatt tacttggaag atttggaggg aaatggcatt gaactctatc
     7801 gagataagcc agtttccaca tgggatattc gagaagatgg acgcattatc ggagtgacgg
     7861 aggctcttgc ggcgcaggac atttatgagt tgggggaaag agtagagccc tttatcctgg
     7921 cagaaggtac gagaatgggg catattcatc tttccgtcaa ggatagtcga aagtccagcc
     7981 agttttatca aaaggtgtta gggctagagg ataaattcag tgtgcctagc gctagttgga
     8041 ttgcggctgg agattaccat catcatttag cagttaacga atggggagga aaaggtctgg
     8101 cttcgcgtaa gcaaggctta ccaggtttag cctactatgt catcgaagtc gcacataaag
     8161 aagaactgtt aacgattgcc caacgagcac aagaagttga cgcaccaatc aaatggatga
     8221 catcgagcca attggaaatc acagactcag atggcatcgt gacccgtatt cgtttagcag
     8281 ggtaaaatta ggaatttcta ataagttata agtattgtta gtttgctttt tgaacggtct
     8341 ttggtaacag aactgcgtga gctggatggg cgagaatttt gacaaagaag ctgattagag
     8401 atataatgaa gaaaaatcga gcaaggaaaa agaaatgaca aattcaaagt atattacttg
     8461 tttgaaacgt tcagagggcc agttgtgtgg gattcaaaag atgattgaag gagatcgtga
     8521 ctgtgctgat attgtgacgc aactgacagc agtgagatct agtgtagagc gcgtgattga
     8581 gatgataatt accgaaaatc ttactgaatg tatcaatcag ccactagatg attctgaagc
     8641 tcaaaaggaa cgcctagaaa aggctatccg atacttgatt aaacggaaat aaatgaatgt
     8701 tttgagacga tatgtgcaaa ggtctattaa ctattagaat agtttaatgg gagtcaaaag
     8761 aagttgtaaa aaatctttaa aattaaaaaa acgcatagta tcaggtgttg aatgtattga
     8821 ccccaaaaag ttagattttt tctgtctaac ttttggggtg cagttcataa gaaccttggt
     8881 aatatgcgtt ttattatgta gagacttata ctcttcgaaa atctcttcaa accacgtcag
     8941 cttcaccttg ccgtagatat ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca
     9001 gtgttttgag ctgacttcgt cagttccatc cacaacctca aaacagtgtt ttgagctgac
     9061 ttcgtcagtt ccatccacaa ccttaaaaca gtgttttgag ctgacttcgt cagttccatc
     9121 cacaacctta aaacagtgtt ttgagcagcc tgcggctaac ttcctagttt gcactttaat
     9181 tttcattgag tattacttca tcttctcatg aaattgagtt tttgcccagc tcctcttgat
     9241 tattctactt gaccgggcca agcattcata ccaccttcta cgttggtaac ggtgaggcct
     9301 tgggagctga gaaattggca ggtagatgca gagcggactc ctccttgaca aatgacatag
     9361 tattcatggt caggtttgag ttctttgtag ccttgctcta aggtacttaa tggaagattt
     9421 ttggcacctg gtgcatgtcc tgcttggaat tcatgtgctt cacggacatc gataagttct
     9481 agattttcat tttggtattt tccataaaag tcagccatgc tgatactagt ttccatattc
     9541 tactccttct gggttaacca ttttgtatag tgaataagcg ccgtcaaggt tttgggcggt
     9601 aaatcctgct tgcttgagga taggctctgc gatatagctg cgcaaaccac tgtggcagct
     9661 gacgatgtag gcttgttttt tgtccagttc atcagcttcc tccttgacac tttgtcagtt
     9721 tttgatacaa tagtacaaaa ttagaggagg caggctatga ttcagaaaca tgcgattcct
     9781 attttagagt ttgatgacaa tcctcaggcg gttatcatgc ccaatcacga ggggctggac
     9841 ttgcagttgc caaagaagtg tgtttatgca tttttaggtg aggagattga ccgctatgca
     9901 agggaagtag gggcggattg tgtcggcgaa ttcgtttctg ctaccaagac ctatccagtc
     9961 tatgtcatca actacaaggg tgaggaggtc tgtctggctc aggctcctgt tggctccgct
    10021 ccagcagccc agtttatgga ttggttgatt ggctatggtg tggagcagat tatctctact
    10081 gggacctgtg gtgtcctagc tgatatagag gaaaatgcct ttctagtccc tgttcgcgct
    10141 ttgcgagatg agggagccag ttaccactat gtggcacctt gtcgttatat ggaaatgcag
    10201 ccagaggcta ttgctgctat tgaggaagtt ttggaagaca gagggattcc ttatgaagaa
    10261 gtcatgacct ggacgacaga cggtttttac cgagaaacgg ctgaaaaggt ggcttatcgt
    10321 aaggaagaag gctgtgctgt tgtggagatg gagtgttctg ctcttgcggc agtagctcaa
    10381 ttgcgtgggg ttctctgggg tgaattgttg ttcacagcag attctctagc ggacttggac
    10441 cagtacgaca gtcgttactg gggctcggaa gcttttaata aggcgctaga actgagttta
    10501 gcaagtgttc accaccttta gttgtactgg caaaggattt gttttatcat aaaatgtcta
    10561 gctcatactt ttcaaaaata tgtttaaacg aggtcacctt cctcttgtcc taggcatgtt
    10621 gaggttggga aaaatcttta aaatcagaaa aacgtatcat atcaggtgat gaaaactttg
    10681 atactatgcg ttttatgttg gtaagattta gagtgagatg aaatgatact cttcgaaaat
    10741 cttttcaaac caggtcagct tcaccttgcc gtatagatgt tactgacttc gtcagttcta
    10801 tctgcaacct caaaacagtg ttttgagctg acttcgtcag ttctatccac aacctcaaaa
    10861 cagtgttttg agcaacctgt gactagcttt ctaagcgatg ccttggttct cattgcctat
    10921 aatcaaaaag agaaattttc tcctgaaaag catatagagt agctggcgtt aaaagctcct
    10981 gtcttgcttt tttgacctat agtcacatct atcaagtatt gttcttgcct aagctatcaa
    11041 taaaaaggtg gcatttttta ggcttggtgt tagtgaattt tgccttatcc tatctaagtc
    11101 atttcgagct ttttatggta caatggaaac atgttattca aattatctaa ggaaaaaata
    11161 gagctaggct tatctcgttt atcgccagcc cgtcgtattt ttttgagttt tgccttggtc
    11221 attttactag gctctcttct tttgagcttg ccctttgtcc aagttgaaag ctcacgagcg
    11281 acttattttg atcatctttt cactgctgtc tctgcagtct gtgtgacagg tctctcaacc
    11341 cttccagtag ctcacaccta taatatctgg ggccaaataa tctgtttgct cttgattcag
    11401 atcggtggtc tagggctcat gacttttatt ggggttttct atatccagag caagcaaaag
    11461 cttagtcttc gtagccgtgc aactattcag gatagtttta gttatggaga aactcgatct
    11521 ttgagaaagt ttgtctattc tatttttctc acgacctttt tggttgagag cttgggagct
    11581 attttgctta gttttcgcct tattcctcaa cttggctggg gacgtggtct ttttagttcc
    11641 atttttctag cgatctcagc cttctgtaat gccggttttg ataatttagg gagcaccagt
    11701 ttatttgctt ttcagaccga tttactggtc aatctggtga ttgcaggctt gattattaca
    11761 ggcggccttg gttttatggt ctggtttgat ttggctggtc atgtaggaag aaagaaaaaa
    11821 ggacgtctgc actttcatac gaagcttgta ctattattga ctataggttt gttgttattt
    11881 ggaacagcaa ctactctctt tcttgagtgg aacaatgctg gaacgattgg caatctccct
    11941 gttgccgata aggttttagt tagctttttt caaacagtga cgatgcgaac agctggcttt
    12001 tctacgatag attatactca ggctcatcct gtgactcttt tgatttatat cttacagatg
    12061 tttctaggtg gggcacctgg aggaacagct gggggactca agattacgac attttttgtc
    12121 ctcttggtct ttgcacgaag tgagcttcta ggcttgcctc atgccaatgt tgcgagacga
    12181 acgatcgcgc cgcgaacggt tcaaaaatcc tttagtgtct ttattatctt tttgatgagc
    12241 ttcttgatag gattgattct gctagggata acagccaaag gcaatcctcc ctttatccac
    12301 ctcgtatttg aaaccatttc agctcttagt acagttggtg taacggcaaa tctgactcct
    12361 gaccttggga aattggctct cagtgttatc atgccactta tgtttatggg acgaattggt
    12421 cccttgacct tgtttgttag cttggcagat taccatccag aaaagaaaga tatgattcac
    12481 tatatgaaag cagatattag tattggttaa gaaaggaaag agcatgtcag atcgtacgat
    12541 tggaattttg ggcttgggaa tttttgggag cagtgtccta gctgccctag ccaagcagga
    12601 tatgaatatt atcgctattg atgaccacgc agagcgcatc aatcagtttg agccagtttt
    12661 ggcgcgtgga gtgattggtg acatcacaga tgaagaatta ttgagatcag cagggattga
    12721 tacctgcgat accgttgtag tcgcgacagg tgaaaatctg gagtcgagtg tgcttgcggt
    12781 tatgcactgt aagagtttgg gggtaccgac tgttattgct aaggtcaaaa gtcagaccgc
    12841 taagaaagtg ctagaaaaga ttggagctga ctcggttatc tcgccagagt atgaaatggg
    12901 gcagtctcta gcacagacca ttcttttcca taatagtgtt gatgtctttc agttggataa
    12961 aaatgtgtct atcgtggaga tgaaaattcc tcagtcttgg gcaggtcaaa gtctgagtaa
    13021 attagacctc cgtggcaaat acaatctgaa tattttgggt ttccgagagc aggaaaattc
    13081 cccattggat gttgaatttg gaccagatga cctcttgaaa gcagatacct atattttggc
    13141 agtcatcaac aaccagtatt tggataccct agtagcattg aattcgtaaa gagggatgac
    13201 ccctcttttt tgatgcctaa gatggcaaat agagacagaa gccccttgtc ttctagtaaa
    13261 agttcttcaa aggctggact ttatggtaaa atagaaagaa gtgacaagag agagtaatac
    13321 tcaatgaaaa tcaaagatca aactaggaaa ctagctacgg gctgctcaaa acactgtttt
    13381 gaggttgcag atagaactga cgaagtcagt aacatctata cggcaaggcg acgttgacgc
    13441 ggtttgaaga gattttcgaa gagtataaga aaaaatcagt cccctaaagg agtagattat
    13501 gaagttattg tctatcgcaa tttctagcta taatgcagca gcctatcttc attactgtgt
    13561 ggagtcgcta gtgattggtg gtgagcaagt tgggattttg attatcaatg acgggtctca
    13621 ggatcagact caggaaatcg ctgagtgttt agctagcaag tatcctaata tcgttagagc
    13681 catctatcag gaaaataaat gccatggcgg tgcggtcaat cgtggcttgg cagaggcttc
    13741 tgggcgctat tttaaagtag ttgacagtga tgactgggtg gatcctcgtg cctacttgaa
    13801 aattcttgaa accttgcagg aacttgagag caaaggtcaa gaggtggatg tctttgtgac
    13861 caattttgtc tatgaaaagg aagggcagtc tcgtaagaag agtatgagtt acgattcagt
    13921 cttgcctgtt cggcagattt ttggctggga ccaggtcgga aatttctcca aaggccagta
    13981 taccatgatg cactcgctga tttatcggac agatttgttg cgtgctagcc agttctaact
    14041 gcctgaacat actttttatg tcgataatct ctttgtcttt acgccccttc agcaggtcaa
    14101 gaccatgtac tatctgcctg tcgatttcta tcgttatttg attgggcgtg aggaccagtc
    14161 tgtcaatgag caagtgatga ttaagtgcat tgaccagcaa ctcaaggtca atcgactctt
    14221 gatagaccaa cttgatttgt cccaagtgag tcatcccaaa atgcgagaat atctgctgaa
    14281 tcatattgaa ctcacgacgg tgatttccag taccctgctc aaccgatctg gaacagcgga
    14341 gcatctggca aaaaaacgcc aattgtggac ctatattcag cagaaaaatc cagaagtctt
    14401 tcaggctatt cgtaagacca tgttgagccg tttgaccaaa cattctgtct tgccagatcg
    14461 caaactgtcc aatgtcgtct atcaaatcac caaatctgtt tatggattta attaatataa
    14521 gtgttttata agagggattt aagaaaaatt ttaacttttt cttagtcctt tttaatttca
    14581 ggagattata ctagagtcat caaataaaga aagactctaa ggagaatcct atgaaattca
    14641 atccaaatca aagatatact cgttggtcta ttcgccgtct cagtgtcggt gttgcctcag
    14701 ttgttgtggc tagtggcttc tttgtcctag ttggtcagcc aagttctgta cgtgccgatg
    14761 tggtcaatcc gaccccaggt caagtcttac ctgaagagac atcgggaacg aaagagggtg
    14821 acttatcaga aaaaccagga gacaccgttc tcactcaagc gaaacttgag ggcgttactg
    14881 gaaatacgaa ttcacttccg acacctacag aaagaactga agtgagcgag gaaacaaact
    14941 cttctagtct ggatacactt tttgaaaaag atgaagaagc tcaaaaaaat ccagagctaa
    15001 cagatgtctt aaaagaaact gtagatacag ctgatgtgga tgggacacaa gcaagtccag
    15061 cagaaactac tcctgaacaa gtaaaaggtg gagtgaaaga aaatacaaaa gacagcatcg
    15121 atgttcctgt tgcttatctt gaaaaagctg aagggaaagg tcctttcact gccggtgtaa
    15181 accaagtaat tccttatgaa ctattcgctg gtgatggtat gttaactcgt ctattactaa
    15241 aagcttcgga taatgctcct tggtctgaca atggtactgc taaaaatcct gctttacctc
    15301 ctcttgaagg attaacaaaa gggaaatact tctatgaagt agacttaaat ggcaatactg
    15361 ttggtaaaca aggtcaagct ttaattgatc aacttcgcgc taatggtact caaacttata
    15421 aagctactgt taaagtttac ggaaataaag acggtaaagc tgacttgact aatctagttg
    15481 ctactaaaaa tgtagacatc aacatcaatg gattagttgc taaagaaact gtcgaaaaag
    15541 ctgttaaaga taacgttaaa gacagtatcg atgttccagc agcctaccta gaaaa
//
LOCUS       DAVKGC010000040        15570 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKGC010000040 DAVKGC010000000
VERSION     DAVKGC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.040"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..263
                     /locus_tag="VW049_001056"
     CDS             <1..263
                     /locus_tag="VW049_001056"
                     /inference="COORDINATES: protein motif:HMM:NF019216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432197.1"
                     /translation="GRFQSFADFKKEYYKELLEKANAGFTPFTIDGETIHNYDELKSL
                     FAKALEEDMVTNKVNGKLVQVKEKVYKKLLQQTNSFKTSIFK"
     gene            324..2045
                     /gene="pabB"
                     /locus_tag="VW049_001057"
     CDS             324..2045
                     /gene="pabB"
                     /locus_tag="VW049_001057"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9432198.1"
                     /translation="MHRKTVIDFRTLGERYTFIQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAENYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     KIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     KHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            2046..2663
                     /locus_tag="VW049_001058"
     CDS             2046..2663
                     /locus_tag="VW049_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9432199.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQYLAQETEPFVLIGLSLGAALALELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKALMVGVSEELKTLDLTDIAGTCPYPTLLICGIKDKPNL
                     SSMRSLHKLISESQFQIISDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            2713..3591
                     /locus_tag="VW049_001059"
     CDS             2713..3591
                     /locus_tag="VW049_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9432200.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGTFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGAYYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGA
                     YYLKSNGKMAQGEWVYDATYQAWYYLKSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGG
                     RYYVGADGVWKEGQASTASSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFD
                     KFSNDAAQYAIDHLDD"
     gene            3718..4677
                     /locus_tag="VW049_001060"
     CDS             3718..4677
                     /locus_tag="VW049_001060"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9432201.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KSLGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            4774..5613
                     /locus_tag="VW049_001061"
     CDS             4774..5613
                     /locus_tag="VW049_001061"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9432202.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLKTNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(5661..5831)
                     /locus_tag="VW049_001062"
     CDS             complement(5661..5831)
                     /locus_tag="VW049_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9432203.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            5952..6836
                     /gene="miaA"
                     /locus_tag="VW049_001063"
     CDS             5952..6836
                     /gene="miaA"
                     /locus_tag="VW049_001063"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9432204.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVLHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRREQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            6829..8067
                     /gene="hflX"
                     /locus_tag="VW049_001064"
     CDS             6829..8067
                     /gene="hflX"
                     /locus_tag="VW049_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9432205.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            8060..8683
                     /locus_tag="VW049_001065"
     CDS             8060..8683
                     /locus_tag="VW049_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9432206.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKSFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            8698..9627
                     /locus_tag="VW049_001066"
     CDS             8698..9627
                     /locus_tag="VW049_001066"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9432207.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAKKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            9648..10403
                     /locus_tag="VW049_001067"
     CDS             9648..10403
                     /locus_tag="VW049_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9432208.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(10457..11365)
                     /locus_tag="VW049_001068"
     CDS             complement(10457..11365)
                     /locus_tag="VW049_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9432209.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            11429..11698
                     /locus_tag="VW049_001069"
     CDS             11429..11698
                     /locus_tag="VW049_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9432210.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            11698..12078
                     /locus_tag="VW049_001070"
     CDS             11698..12078
                     /locus_tag="VW049_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9432211.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            12167..12394
                     /locus_tag="VW049_001071"
     CDS             12167..12394
                     /locus_tag="VW049_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432212.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(12434..13159)
                     /locus_tag="VW049_001072"
     CDS             complement(12434..13159)
                     /locus_tag="VW049_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9432213.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            13321..15162
                     /gene="typA"
                     /locus_tag="VW049_001073"
     CDS             13321..15162
                     /gene="typA"
                     /locus_tag="VW049_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9432214.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            15182..15436
                     /locus_tag="VW049_001074"
     CDS             15182..15436
                     /locus_tag="VW049_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9432215.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT         4588 a         2967 c         3541 g         4474 t
ORIGIN      
        1 acggcagatt ccaatcgttt gctgatttta agaaagaata ttataaagaa ttgttagaaa
       61 aagccaacgc tggttttact ccttttacta ttgatggtga gaccattcat aactatgatg
      121 aattaaaatc attatttgcc aaagctcttg aagaagatat ggttactaat aaagttaatg
      181 gtaaacttgt gcaagtaaaa gaaaaagttt ataagaaact tcttcaacaa acaaatagct
      241 ttaaaacttc aatctttaaa taattgaaat ctctcatctg ctttgcggat gagggttttc
      301 ttattttatg ctatacttaa ggtatgcata gaaaaacagt gattgatttt aggactttgg
      361 gggagagata tacctttatc cagcctatta aagagttgaa aacgagaaat gtagaagaag
      421 tggcagattt gctggcacaa gtggaaagct accaagagca aggttattat gtggtggggt
      481 atgtcagcta cgaggctgca cctgcttttg aggaaaaatt agcagttcac aaggatcccc
      541 tactgggaga gtacctgctt tactttactg ttcatgatag ggtagagacc tcccctattc
      601 ctctgactta tgaggatatt gatttgccct caaattggca ggaagtaacg tctgcagaga
      661 actatgaaaa ggccattgcc cagatacacc atcacttgag gcagggagac acctatcagg
      721 tcaactacac cgtccaactc aagcaaaagt taagtgccaa tccttttgcc atctacaatc
      781 gtatggtggt agagcaggaa gcgggctaca atgcctatgt ggaacatgac gagatggcag
      841 tgatttccat gagcccagag ctcttttttg agcaaaatga tcgcgagttg acaacacgac
      901 caatgaaggg gacgactcag cgtggggtaa ctgaccaaga agatcttgaa caggccagtt
      961 ggttggaaca ggatcccaaa aatcgctctg aaaatatgat gattgtggat ctcttgcgca
     1021 atgatatgaa ccgtatttct gaagttggga gcgagcacgt ggagcgtctg tgccaggtag
     1081 agcagtattc aactgtttgg cagatgactt cgaccatcaa gagtcagttg cgagaggatg
     1141 tggaccttgt taaaatcttc cgctcactct ttccttgtgg ttccataacg ggtgcaccga
     1201 aaattgcgac aatggagatt atcaaggact tggagcctca accgcgtgga gtctactgtg
     1261 gaacgattgg tctcttgctt ccaaatggac gacggatttt taatgtggcc attcgtacca
     1321 ttcaacttca caaaggtcaa gctatttatg gagttggcgg agggattact tgggatagca
     1381 catgggaatc tgaataccga gaggttcatc aaaaggcagc agttctttat cgtaaacaag
     1441 ctcgcttcca actgattacg acaggggaaa tcagccagaa gaacctgctg tttgaagata
     1501 aacatctgga aagactgaga aaagctagtc gttattttgc ctttcctttt gatgcagaag
     1561 acttgggaca caagattgag gaagagtgtc aggattgtga agctaatcaa gattaccgct
     1621 tgcgaatcag ccttagcaaa tctggagaga tagaagtcaa tcgtcaagta ttaacccctc
     1681 tcagtacaag cttttgtcag gcccaagtct gccttcagga agctgctttg aatcaatcct
     1741 ttacctactt taaaaccact caccgaccgc atttgagcct aggagaacaa gagaagattt
     1801 accacaataa gtcaggagaa ctgcttgaaa cctctatagg aaatttggtt ctgaaaatcg
     1861 ctggaaaact ctacacaccg cctatccgac ttggaatctt gccaggaatt taccgtcagt
     1921 atttgctaga aacaggacag gtagaagaga aagtcttgac cttggcagat ttagcccaag
     1981 cagaagctat ttacggctgt aatgcagtga gaggcttgta tgaactaagc cttgaggaga
     2041 actagatgaa actgatattt ttgcatgggt tagggcagtc agcagagtct tggaaagaag
     2101 ttcggaatct actgacagat tatccttctg aggctatcga gttatttcct tctggagtta
     2161 gtaactatca acaagctaag gagcgagttt atcagtacct agctcaagag acagaacctt
     2221 ttgttttgat tggattgtcc ttaggtgctg ccctggcact agaactttcc agttacgatt
     2281 taccaaatct tcgagctttg attctgtcag gatgcccgct taaattagca gggaatatcc
     2341 tattttatct tcagttgctg atttttaaac tacttcccaa aagagtattt gaaaaacagg
     2401 gagcagacaa ggctcttatg gtcggagttt ctgaggagtt gaagacactt gatttaacgg
     2461 atatagcagg aacttgccct tatccaacct tactaatttg tggtatcaaa gataaaccga
     2521 atcttagttc tatgagaagt cttcataaac taatatcaga atcccaattt cagattatct
     2581 cagatggccc tcatgtcttg aataaggcaa aaccaaagga gtttgtagaa aagaccagaa
     2641 gtttccttga attgctgaaa taagtttgat aaaataatat tgaaatcgat atcataatta
     2701 tgggagaaag aaatgaataa acgtctattt ttaaaaatga gtctggtgac gttgccaatt
     2761 ttagccttgt tttcacaacc tgtgttagca gaggaaaaca ttcatttttc gagttgtaag
     2821 gaagcttggg ctaatggata ttcggatctt catgagggag aacctggtta ttctgccaag
     2881 ttagaccgcg atcatgatgg tgtggcttgc gaattgaaaa atgctcctaa gggtactttt
     2941 aaagcaaaac aagcggctac aactcaaact gatacaactt catccacagc aagtggttgg
     3001 gttaagcagg acggttcttg gtactatttt gatggaaatg gaaatctagt gaaaaatgca
     3061 tggcaaggag cttattacct taaatcaaac ggtaaaatgg cacaaggtga gtgggtttat
     3121 gattcttctt accaagcatg gtattacttg aaatcagatg gttcttatgc tcgcaatgca
     3181 tggcaaggag cttattacct taaatcaaac ggtaaaatgg cacaaggtga atgggtttat
     3241 gatgccacct atcaagcttg gtattacttg aaatcagatg gttcttatgc ttacagtaca
     3301 tggcaaggaa attactatct aaaatcggat ggtaaaatgg ctgtcaatga atgggttgat
     3361 ggtggacgtt attatgttgg tgctgacgga gtttggaagg aaggtcaagc aagtacagct
     3421 tcttctagta atgatagcaa tagtgaatat tctgctgctt taggaaaagc aaaaagttat
     3481 aattcgttat tccatatgtc aaaaaaacgt atgtatagac aattaacttc tgattttgat
     3541 aaattttcaa atgatgcagc tcaatatgcc attgatcatt tagatgatta acagatactg
     3601 gtaggcgaaa aaattcctta taattcattt acgcaaacct ttgcataagt gaatggattt
     3661 tgatatacta gtactgtaag cattttcaat tactacacaa tgaaaaagga gaatatgatg
     3721 agtcaaaaga ttattgggat tgaccttggt ggaacttcta tcaaatttgc aatcttaaca
     3781 acagcaggag aaatccaagg aaaatggtca atcaagacca acattttgga tgagggaagt
     3841 catatcgttg atgatatgat tgagtctatt caacatcgtt tggacttgct tggattggca
     3901 gcagcggact tccaaggcat tggaatggga tcaccaggtg tggttgaccg tgacaaaggg
     3961 actgttatcg gtgcctacaa cttgaactgg aaaacccttc aaccaatcaa acaaaagatt
     4021 gaaaaatctt tgggcattcc atttttcatc gataatgatg ccaacgtagc agctcttggt
     4081 gagcgctgga tgggtgctgg tgataaccaa ccagatgttg tctttatgac actcggtact
     4141 ggtgttggtg gcggtatcgt cgcagaaggc aaattgcttc acggtgttgc tggtgcagca
     4201 ggtgagcttg gtcacatcac tgttgacttt gaccagccaa tctcatgtac ttgcggtaag
     4261 aaaggctgtc ttgagacagt cgcttctgca acaggaattg tcaacttgac tcgtcgctat
     4321 gccgatgaat acgaaggcga tgcagccttg aaacgcttga ttgataacgg agaagaagta
     4381 actgctaaga ctgtctttga tctcgcaaaa gaaggagacg accttgcttt gattgtttac
     4441 cgtaacttct cacgttactt gggaatcgct tgtgctaaca ttggttcaat cctaaaccca
     4501 tcaacaatcg tcatcggtgg tggtgtgtca gctgcgggag aattccttct acaaggtgtt
     4561 caaaaagtct atgacgaaaa tactttccca caagtacgca catctactaa attagctctt
     4621 gcaactctag gaaatgacgc tggagttatc ggagcagcat cacttgtatt gcaataagat
     4681 aatgagagag gaaaatgcta acctagagtt agtaatgttc ctcttttatt ttttatgcta
     4741 tactagttag gacaatgaat gaggtgagag taaatgacaa aagcagatac gatttttaaa
     4801 gagaatattg aacgcattct aaaggaaggt gttttctcag agcaggctcg tcctaagtac
     4861 aaggatggga ctgttgctaa ttccaagtat gtaactggtg ccttttccga gtacgactta
     4921 tctaaaggag aatttcctat cacaaccttg cgtcccattg caatcaaatc cgccatcaaa
     4981 gaagttctct ggatttacca agaccagtca aatagcttag aagtgcttaa tgacaagtac
     5041 aatgttcact actggaatga ctgggaagtt ggagacacgg gaaccattgg tgagcgctat
     5101 ggtgccgttg ttaagaaaca cgacattatc aataagcttc tcaaacagtt gaaaaccaat
     5161 ccttggaacc gccgcaatat tatttctctc tgggattacc aagctttcga agaaacagat
     5221 gggctgctcc cgtgcgcctt tcagaccatg tttgatgttc ggcgtgttga tggggaaatc
     5281 tatctggatg cgaccttgac ccagcgctcc aatgatatgc tggtggccca ccacatcaac
     5341 gctatgcagt atgtggcttt gcagatgatg attgccaaac attttggctg gaaggttggg
     5401 aagttcttct acttcatcaa caacctccat atctatgata atcaatttga acaagctcag
     5461 gaattgctcc gtcgggagcc gtcaaactgc caaccacgct tggttttaaa tgttcctgat
     5521 gggactaatt tctttgatat caaagcagaa gattttgagt tggtggatta tgaccctgtt
     5581 aagccacagt tgaagtttga cctagctatt taaaagaata gaaaaaagaa gttgagaata
     5641 atcccaactt cttttgtttc ttaacgtgat acgcggcgac gagctgcttt tttacggttt
     5701 tcttcgatga aagctgcttt ttgctcttct ggttcgatta ctttcttttt aaatgcgtat
     5761 actgcacctg caacggcagc gacagttcct gcgacacctg ttacaagacc tttagcgaat
     5821 cctttagcca tgagtcttcc tcctttatat tctcaatcag ccagcctcct caagaggtca
     5881 catttttctg actgaccttt ttgtgttata ataatagtaa cgaaaaaatg ggaatttttc
     5941 aaggaaaaaa gatgaaaaca aaaataattg tgattgttgg accgactgct gttggaaaga
     6001 cggctctagc tattgaagtt gcaaagcgtt ttaatggcga agtggttagt ggagatagcc
     6061 agcaagtcta tcgaggactt gatattggga cggccaaggc tagtccagaa gagcaggcag
     6121 ctgttcttca tcatttaatc gatgttagag agataaccga gtcttactcg gcttttgatt
     6181 ttgtttcaga agctaagatg actattgagg atattcacag ccgtggcaag ctagccatta
     6241 tcgccggtgg gactggactt tatatccaga gcttgctaga aggttaccac ctaggtgggg
     6301 agactcctca tgaggagatt ttagcttatc gagctagttt ggagccatat tcagatgagg
     6361 aattagccca tttggtggag caagcaggcc ttgagattcc ccagtttaat cgtcgtcgtg
     6421 ctatgcgtgc cttagaaatt gcccattttg gtcaggattt ggaaaatcaa gagaccttgt
     6481 atgaaccgct gattatctgc ttggatgatg aacgtagtca actttatgag cgtatcaacc
     6541 atcgagtgga tttgatgttt gaggctgggc ttttggatga ggccaagtgg ctgtttgacc
     6601 attcccctaa tgtacaggct gctaaaggaa ttggctataa ggaactcttt ccttatttcc
     6661 gtagagagca gaccttggag gaagctcgtg agagtctcaa acaggctact cgtcgttttg
     6721 ccaagcgtca gctgacctgg ttccgtaatc gcatgcaggt caccttttat cagattggag
     6781 aatctggtgt acaagaccgc attttaagcc agatagagga gtttttagat gattgaaacg
     6841 gagaaaaaag aggagcgggt cctgctgatt ggtgtggaat tgcagggtat ggacagtttt
     6901 gacctctcca tggaagaatt ggctagttta gcgaaaacgg caggggcagt cgttgtagat
     6961 agctacagac aaaaacgtga aaaatatgat tccaagacct tcgtcggctc tggtaagttg
     7021 gaagagattg cgcttatggt ggatgcagaa gaaatcacta ctgtcatcgt caacaaccgt
     7081 ctgaccccaa ggcagaatgt caatctagag gaagttctcg gtgttaaggt cattgaccgt
     7141 atgcagttga ttttggatat ctttgccatg cgggctcgaa gccatgaagg gaagctccaa
     7201 gtccacctag cccaactcaa atacctcttg cctcgcttgg ttggtcaggg gattatgctc
     7261 agccgtcagg cagggggaat tggttcccgt ggtcctggtg aaagccaact ggagctgaac
     7321 cgtcgtagcg ttcgcaatca aatcacggat atcgagcgcc agcttaaggt ggttgagaaa
     7381 aatcgtgcga ctgtcagaga aaaacgtttg gagtctagca cctttaagat tggtttgatt
     7441 ggttatacca atgctgggaa atcaactatc atgaacatct tgaccagtaa gacccagtat
     7501 gaagcagatg agctctttgc gactctggat gcgacaacca agagtattca tctaggaggc
     7561 aatctccaag taactttgac agataccgtt ggctttatcc aagatttgcc gacagagttg
     7621 gtgtccagtt tcaagtcaac cttggaagaa agcaagcatg tggaccttct ggttcatgtt
     7681 atcgatgcta gcaatcctta ccacgaggag catgaaaaaa cggttctctc catcatgaaa
     7741 gacctggaca tggaagatat tcctcacttg acgctttata ataaagcgga tttggtggag
     7801 gatttcacgc ctactcaaac gccatatacc ctcatttctg ccaagtctga ggacagtcgt
     7861 gaaaacttgc aagcattatt gctagataag attaaggaaa tttttgaagc atttaccctg
     7921 cgagtgcctt tttcaaagtc ctacaagatt catgatttag agagtgttgc aattctggaa
     7981 gaacgtgatt atcaggaaga cggcgaagtg attacaggct acatttccga gaaaaataaa
     8041 tggaggttag aagaatttta tgactgatat taaaactttg gctctaaagt atggaggtta
     8101 tacaagtctg gacaaggtct atctggatca gcttctagct ggtaagacag agcaggagca
     8161 gttaacactt attacacctc cgccaagtgt tgttaatgcc tactttgcag aactctatca
     8221 gaaaaaaagt cctcaggctg cgacggatta ttttgcagaa ctcagtcagg aactgaatct
     8281 ttacaatact gagccaagtt tcaccctaga aaataagtct tttattcggc ttaatctgtc
     8341 tggtaaatcc tttggttttt gttatgagag tgacggcttt ggtcggattt tctctgaaaa
     8401 tgaggagaaa atctcggatg acttgctctt tgaaattgcg caaattttcc cccatcaatt
     8461 agtctttgaa gagtctggta agatttacat gaagcctgtc gaggacgagg aagttgtcag
     8521 cctagaaagt ctcacagctt tgactgattt ggaaagattg gctgatggtc gaaaacgtct
     8581 caaaggctac agccaagagg atttattgca agaagctgct gctttttctg gcaaacgcta
     8641 tttccgatcg gaaaaccgca cagccatgtt atatattgat taattagaaa gtatcgaatg
     8701 gatattcaat ttttaggaac gggggctggt cagccctcta aagcccgcaa cgtttcaagt
     8761 ctcgccctga aacttttgga tgagattaac gaagtttggc tctttgactg tggagaaggt
     8821 acgcaaaatc gcattctgga aaccacaatt cgaccacgta aggtcagcaa aatctttatt
     8881 acccatctgc atggagacca catttttggt ttgccaggtt tcctttctag ccgtgccttt
     8941 caggccaatg aagagcagac agatttggaa atctacggac ctcaaggaat caagtcattt
     9001 gtcttaacca gccttcgtgt gtcaggttct cgtctgccct accgcattca tttccatgag
     9061 tttgaccaag attctctggg taaaattctt gaaaccgata aattcactgt gtatgcagag
     9121 gagctggacc acactatttt ctgtgttggc tatcgtgtca tgcaaaagga tctagaaggg
     9181 acgctggatg ctaaaaaact caaggctgct ggtgttccgt tcggcccgct ttttggtaaa
     9241 atcaaaaacg gccaggatct tgttttggaa gacggaactg aaatcaaggc agcagactat
     9301 atctcagcgc cacgtccagg taagattatc actattttag gagacactcg aaaaacggat
     9361 gccagtgtgc gtctggctgt caatgcagat gtcctagttc atgagtccac ttatggcaag
     9421 ggtgatgaaa aaattgctcg taaccatggt cactcaacta atatgcaagc tgcacaagta
     9481 gcggtagaag caggtgccaa acgcctccta ctcaaccata tcagtgcccg tttcctctca
     9541 aaagatataa gcaaactcaa gaaagacgct gccacaattt ttgaaaatgt ccatgtggtc
     9601 aaagacttgg aagaagtgga aatctagcag tcacagaaag gataagtatg cctactattc
     9661 tcattaccgg agctagcggt ggtctagctc aagaaatggt caaactcctg cccaatgatc
     9721 aactcatctt gcttggtaga aataaggaaa aattagccca actctacgga aattattccc
     9781 atgcagaatt gattgaaatt gatattaccg acgattcagc cctagaagct ctggtaactg
     9841 atctttatct ccgctatggc aagattgatg tcttgattaa caacgctggt tacgggattt
     9901 ttgagggatt tgaccagatt gctgataaag atattcacca gatgtttgag gtcaatacct
     9961 ttgccctgat gaatctgtct cgtcaccttg cggctcgtat gaaggaaagc agcaaagggc
    10021 atatcatcaa catcgtcagc atggcaggtc taatagctac tggcaagtct agtctttact
    10081 cagcgaccaa gtttgcggct attggttttt caaatgctct gcgactcgaa cttatgccct
    10141 atggagtcta tgtgacaaca gtcaatccag gtccaatccg aacaggattt tttgaccaag
    10201 ctgacccaga tggaacttat cttaaatcgg ttgaccgctt cctcttagag gcagatgcag
    10261 tggctaaaaa gattgtcaag attataggca aaaataaacg agaactcaat ctcccgattt
    10321 tgttgaacct agcccataag ttttatactc tctttcccaa gctagctgat aagttggcag
    10381 gggaaacttt taattataag taaaaagaac caatgtgcag gttgttgcta gcctacatat
    10441 tggttcttat catttctcaa ctatcaaact gaacttcttc tagtagatag tcgataaagc
    10501 gttcgcccat cttagatagg ctggttttct catgctggat atagaccagc tcaatcgggt
    10561 catcaatatc cagtgggata gaaacgatat tgtctccgtt taggttgctg ttcaaaatcc
    10621 ctgtcgcaat ggtataacca tccaaaccaa tcaagagatt aaagagggtg gcacggtcac
    10681 tgaccacaat ggatttcttg tggtgttctt gagaaagaat ctcttctgaa aagtagaagg
    10741 agttgtgcgt cccttggtca tagctgaggt aagggaaatt ctccaaatca gacagtttca
    10801 ccttgtcttt ctttgccaga gggttggtct tgctgacaaa gatatgcggt tgcgctgtga
    10861 agagatggtg ggctagcagg tgattgtcat ccagcatctt ggttaaaaca tcacggttgt
    10921 aactgtttaa gaagaggacc ccgacctcac tgcggaagtt cttgacgtcg tcgataatct
    10981 cccaagtccg agtttcacga aggaagagtt cgtatttctc catatcgctt ttcttgagca
    11041 aagagacaaa ggcattgacc acaaaggcat agtgttgaga cgaaacgcta aagagttcgc
    11101 ggtgggcgac aggattttta tagcgttcct ccagaagctg ggtctgctcg acaacctgac
    11161 gggcataaga gagaaactcc atcccatcac gggtcaaggt gattcccttg ggattgcgga
    11221 taaagatctc aatgcccatt tcattttcca aatctcgcac tgcattggag agacttggct
    11281 gagtgataaa gagttgcttg gctgcctcat tcatggagcc agtttcgacg attttgataa
    11341 tataatgtaa ttgttgaatt ctcatgtttt tattgtacca tacttgcctt agaatcgcta
    11401 aagaagatag gaaaataagg agcattgtgt gagtctcttc ttgaaaaaaa cacaaaattt
    11461 tgctagaatg agtcttatga aaacattcta tgatgtgcag caattcctca aacgatttgg
    11521 tattattgtt tacatgggaa aacgattata tgatattgaa ctgatgaagt tggaactatc
    11581 tcggatttac gatgcagggt tgatggataa attagactat ctagaggcag aagcagttct
    11641 tcgcagagag cacaaggtag aattggatta tattgagaaa aatggagaaa agaactaatg
    11701 gttacttgga ttttgtgggc acttatacta gcaatgttgg cgtggatggg ctttaactat
    11761 cttcgtattc gccgtgcggc taaaattgtg gacaatgagg agtttgaagc cttgattcgt
    11821 acgggtcaat tgattgattt gcgcgaccca gcagaattcc acagaaaaca tatccttggt
    11881 gcacgcaata ttccttcaag tcagttgaaa actagtcttg cagcccttcg taaagataaa
    11941 cctgtccttc tctacgaaaa ccaacgtgcg caacgagtta caaatgcagc tctttacttg
    12001 aaaaaacaag gtttttctga gatttatatc ctttcttatg gcttggattc ttggaaaggg
    12061 aaagtgaaga ctagctaacc ttatttccaa attagtcgaa tgataggatg aagatattgt
    12121 tttatagtat aattaatttg tataaaattt aaggagttct tagaacatga aagaaaagaa
    12181 gaaaaatcca ctttttgtgg gtatcttgtc gattattctt ggtttgttat ttccaattgt
    12241 aggcctgatt ttgggtatca taggattggt cttggctatt tcataccaaa aagagtcaca
    12301 attagactat aaaatagaaa agattcttaa tatcttagga attgtgattt ctgtagttaa
    12361 ctggattgta gcaatcgcct taatttttcg gtaaataaaa gaaaaaagct ttagttttaa
    12421 agcttttttt gttttatcga ctcatctcta aatagttttt aatgatttgt aactcttttt
    12481 ggttcaaagg gcggtagtta ccttctgcta aatctgggtt caatgtgaag tccccaaatt
    12541 ggattctttt gaggctagtc accttaacac caaccgagag gaacattttc ttgatttgat
    12601 gaaattttcc ttctgaaatg gtgatagagg cttgactgag ggagggactt gcagatagaa
    12661 tctctagttt tgcgggttta cagacagtac catctaaaaa gacaattccc ttttgaaagg
    12721 cttggatatg gtcaggtgtt agaagtccat taacctggac ttggtaagtc ttatcgacat
    12781 gatattgggg atggaggagc tgaaagccca agggaccgtt atcggtcaag aggaggagtc
    12841 ccgttgtatc tcggtccagt cggccaacgg catagagctt gtcagactgg atgtcagatg
    12901 gaagcaggtc catgacggtc ggaagttcct tgtctttgtt ggctgtaacg acaccagcag
    12961 gtttatgaag cataagatag gtgtgttcat agccttgaat gattcgatcc tgaaaaagga
    13021 gttcttgtag tcctgtatcg atattttgag ctagggatcg ggctgggcaa ccatcgacta
    13081 gaatttcccc tctgaggagt gcttgcttca tggcctttcg gctaactttt tcttgggcta
    13141 ataaattatc taaacgcata ctgtgcctat cttatcataa gtcgcttgct ttgaaaaggc
    13201 ttgacgtgaa aagaaaagcg gagtgaaaac atttacactt gcttgagtta tgttatttat
    13261 ttgaaattat ggtataatcg ttcagttaga aaataaattt tgaatattat agaggaaatc
    13321 atgacaaaat taagagaaga tatccgtaac attgcgatta tcgcccacgt tgaccacggt
    13381 aaaacaaccc tggttgacga attattgaaa caatcagaaa cgcttgatgc acgtactgaa
    13441 ttggcagagc gtgctatgga ctcaaacgat atcgaaaaag agcgtggaat caccatcctt
    13501 gctaaaaata ctgccgttgc ttacaacgga actcgtatca acattatgga cacaccagga
    13561 cacgcggact ttggtggaga agttgagcgt atcatgaaaa tggttgacgg tgttgtcttg
    13621 gtcgtagatg cctatgaagg aaccatgcca caaactcgtt tcgtattgaa aaaagccttg
    13681 gaacaagacc ttgtcccaat cgtggttgtt aacaaaatcg ataagccatc agctcgtcca
    13741 gcagaagtag tggatgaagt cttggaactt ttcatcgagc ttggtgcaga tgacgaccag
    13801 cttgatttcc cagtggttta tgcttcagcg atcaacggaa cttcttcatt gtcagatgat
    13861 ccagctgacc aagaagcgac tatggcacca atctttgaca cgattatcga ccatatccca
    13921 gctccagtag ataactcaga tgagcctttg cagttccaag tgtcactttt ggactacaat
    13981 gacttcgttg gacgtatcgg tatcggtcgt gtcttccgtg gtacagttaa ggttggggac
    14041 caagttaccc tttctaaact tgacggtaca actaaaaact tccgtgttac aaaactcttc
    14101 ggtttctttg gtttggaacg tcgtgaaatc caagaagcca aagcgggtga cttgattgcc
    14161 gtttcaggta tggaagacat ctttgtcggt gaaaccatca ctccgacaga tgcagtagaa
    14221 gctcttccaa tcctacacat cgatgagcca actcttcaaa tgactttctt ggtcaacaac
    14281 tcaccatttg ctggtaaaga aggtaaatgg gtaacttctc gtaaggtgga agaacgcttg
    14341 caggcagaat tgcaaacaga cgtttccctt cgtgttgacc caactgattc accagataaa
    14401 tggactgttt caggacgtgg agaattgcac ttgtcaatcc ttatcgaaac aatgcgtcgt
    14461 gagggctatg aacttcaagt atctcgtcca gaagttatcg taaaagaaat cgacggtgtt
    14521 aaatgtgaac catttgaacg tgtacaaatc gacactccag aagaatacca agggtctgtt
    14581 atccaaagcc tttctgaacg taaaggtgaa atgttggata tgatttcaac tggtaatggt
    14641 caaactcgtt tggtcttcct tgttccagcg cgtggtttga ttggatactc aactgagttc
    14701 ttgtcaatga ctcgtggtta cggtatcatg aaccatacct tcgaccaata cttgccattg
    14761 attccagggg aaattggtgg acgtcaccgt ggtgcccttg tttctatcga tgctggtaag
    14821 gctacaactt actcaatcat gtctatcgaa gaacgtggta cgatctttgt caacccaggt
    14881 actgaggttt atgaaggaat gatcatcggc gaaaactctc gtgaaaatga cttgacagtt
    14941 aacatcacga aggcaaaaca aatgaccaac gttcgttcag ctactaagga ccaaacagct
    15001 gttatcaaga cgcctcgtat cttgacactt gaagagtctc ttgagttctt gaacgacgat
    15061 gagtacatgg aagtaacgcc tgagtctatc cgtttgcgta aacaaatcct taacaaagca
    15121 gagcgtgaga aagctaataa gaagaaaaaa tcagctgaat aagagctaga aagagagaaa
    15181 gatggtctat ttagtcctag gaattttact gctcctactc tatgtatttg cgacaccaga
    15241 aagcattaaa gggacagtca atatcgtcgc tatggtatgt attttagtgg cactcttgat
    15301 tttattggtt ctatcttttc tgaaaatttt tcaattacca acagaaatat tcctagcaat
    15361 agccatgttg atcctagctt actttagtgt tagagacatc acactcatgc cagtcaaaaa
    15421 aagtaaaaga agataaaaaa gagagtttcg gctctctttt tgctaggaat tttataatag
    15481 tttgaattgc tttttagtta acaccatctc ttttgcttgt atagtatgaa tgggaaaaac
    15541 ttgaataaaa ttcgttaatg gcatctattt
//
LOCUS       DAVKGC010000041        14874 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKGC010000041 DAVKGC010000000
VERSION     DAVKGC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14874
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.041"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..309
                     /locus_tag="VW049_001075"
     CDS             <1..309
                     /locus_tag="VW049_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HEU9432216.1"
                     /translation="LDDRNKAVENLKALEEYLQTRLCAELNKIPDINVQFFDSSQHTL
                     IQIADVFSNHLFRIFKSVAYNQERSGDIKLLRKIHNRNVQYCQYFPANKCQCLHLFSK
                     "
     gene            1312..2478
                     /locus_tag="VW049_001076"
     CDS             1312..2478
                     /locus_tag="VW049_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9432217.1"
                     /translation="MKITEYTKKDGTTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKGGSTVYKAVEIKTYAELVENWLETYCHTVKKATLTIIKSRLNNYLL
                     PAFGEYKLDKLTPPVIQKQVNQWAKEYNQLGKGYQEYPQLNSLNKRILKYAVSLQVIP
                     FNPARDIIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLQNGTLDVSKTLNCEKEVTSPKTKSSIRVIDLDNKTVLMLRLY
                     KNRQAQVGREIGLTYEKVFSNSFDEYRDARALRSRLEKHLKLSDCPRLTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHAKLSMTMDIYSHLSKDNKKNATSFYEKAIEKLKSS"
     gene            2621..3421
                     /locus_tag="VW049_001077"
     CDS             2621..3421
                     /locus_tag="VW049_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9432218.1"
                     /translation="MLPYPFSFFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTVSQASSIVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            3431..4417
                     /locus_tag="VW049_001078"
     CDS             3431..4417
                     /locus_tag="VW049_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9432219.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            4553..4633
                     /locus_tag="VW049_001079"
                     /pseudo
     CDS             4553..4633
                     /locus_tag="VW049_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4753..5694)
                     /locus_tag="VW049_001080"
     CDS             complement(4753..5694)
                     /locus_tag="VW049_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9432220.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(5672..7435)
                     /gene="aspS"
                     /locus_tag="VW049_001081"
     CDS             complement(5672..7435)
                     /gene="aspS"
                     /locus_tag="VW049_001081"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9432221.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANHDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
     gene            complement(7760..7840)
                     /locus_tag="VW049_001082"
                     /pseudo
     CDS             complement(7760..7840)
                     /locus_tag="VW049_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7876..8106)
                     /locus_tag="VW049_001083"
     CDS             complement(7876..8106)
                     /locus_tag="VW049_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432222.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(8124..8765)
                     /locus_tag="VW049_001084"
     CDS             complement(8124..8765)
                     /locus_tag="VW049_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9432223.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRPLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(8777..9511)
                     /locus_tag="VW049_001085"
     CDS             complement(8777..9511)
                     /locus_tag="VW049_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9432224.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(9514..9711)
                     /locus_tag="VW049_001086"
     CDS             complement(9514..9711)
                     /locus_tag="VW049_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432225.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            complement(9711..9917)
                     /locus_tag="VW049_001087"
     CDS             complement(9711..9917)
                     /locus_tag="VW049_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9432226.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(9927..10241)
                     /locus_tag="VW049_001088"
     CDS             complement(9927..10241)
                     /locus_tag="VW049_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432227.1"
                     /translation="MWVLVFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDKKGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(10556..10636)
                     /locus_tag="VW049_001089"
                     /pseudo
     CDS             complement(10556..10636)
                     /locus_tag="VW049_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10627..11916)
                     /locus_tag="VW049_001090"
     CDS             complement(10627..11916)
                     /locus_tag="VW049_001090"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9432228.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(11994..13241)
                     /locus_tag="VW049_001091"
     CDS             complement(11994..13241)
                     /locus_tag="VW049_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9432229.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAVIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLDLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(13432..14283)
                     /gene="rgg"
                     /locus_tag="VW049_001092"
     CDS             complement(13432..14283)
                     /gene="rgg"
                     /locus_tag="VW049_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9432230.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            14407..14748
                     /locus_tag="VW049_001093"
     CDS             14407..14748
                     /locus_tag="VW049_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9432231.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
BASE COUNT         4744 a         3200 c         2728 g         4202 t
ORIGIN      
        1 ctagacgaca gaaataaagc agttgaaaac cttaaagctt tagaagaata cttacaaact
       61 agactttgtg ccgaactaaa taaaatccca gatataaacg tccaattttt cgattcatca
      121 caacatacac taatccagat tgctgatgta ttttcaaatc acttatttag aatattcaag
      181 tctgtagctt ataaccaaga aagatcagga gatatcaaac tacttagaaa aatccataat
      241 cgtaatgttc aatattgcca atacttccca gcaaataaat gtcaatgcct acacttattt
      301 tcaaaataaa aataaaaaag tacaaaaaaa gcgacaaaaa agttgatttt taataaaaaa
      361 aacgatatag tgatgttaga aagtcgcttg taattctgat atgtctgcgt atttttcgta
      421 agttgcctat tgggtaacct gctatcagtt aagggctttt atattttaca aaaaaatcag
      481 tgtatcggct tattatgttg gtacactttt ctttttacta cactagtttt ctacaacaaa
      541 aaaaagacaa gctagctgct gctgacttgt ctaatatcgc ctgaactgaa ataacgggaa
      601 agattcaggc gattagtagc atcttgagag attggaagca gaaaggtctg gttactgttg
      661 agttggggct ctttcaaacc tcttagtcta cgctattatt ataccactct tgcatttaaa
      721 aagcgactga cggcatttct cacgctcatc agtctataac tctctaatga agtataatgc
      781 ggtataattt agacgatttt caacacttcg gcattttagg aacgcttaaa gcgctctaaa
      841 caatatcaaa aaaataaaac tcttgaaata ttggggctca tatgctagaa tgaaagcata
      901 aagggaaaaa cagtgcttct agcatatgta actaaaaagc cccaccgtta cagcgatggg
      961 gttttttgct accctcttaa agggttaaat gctacttgct acgtctcttt atccatagct
     1021 tgaaaagctc agacagtacg ttaacaagta acggagctag aaaaagggaa aacaactcta
     1081 gcatatataa cacctccctt ctctaaattg agggggcgaa aacattatat caaaaacacc
     1141 catcacaaac aagctcatat aagccccata tccgccttgt ttcatattct ggtacaattt
     1201 accgtctgac tgcttaaaat cgaaaatagg ggcattctcg tagcccctcg catggtttaa
     1261 actcaaaacc ttttctaatt gcttgcctgc tgatggaaga ggagtaaaac catgaaaata
     1321 acagaataca caaaaaaaga cggtactaca gtctatcgct ctagcgtcta tcttggtatc
     1381 gataccgtaa caggtaagaa agtcaagacg actatatcag ggcgaaccaa gagagaactc
     1441 aaagccaaag cactacaggc tcaaatagac tttgaaaaag gaggctcaac agtatataag
     1501 gcggtggaga taaaaactta tgccgaactg gtggagaatt ggctggaaac atactgccat
     1561 acagtcaaaa aggcaacact tacaataatc aagagtagac taaataacta tctacttcca
     1621 gcctttggag aatacaaact agataagcta acacccccag ttattcaaaa gcaagttaat
     1681 caatgggcaa aagagtacaa tcaactaggc aaaggttacc aagagtatcc gcaacttaac
     1741 tcgctaaata aacgaatact taaatatgcc gtatccttgc aagttatccc cttcaaccca
     1801 gcccgtgata ttatcgtgcc acgtcgtaag gaaaaagaag ggcaaaaact gaaatatctg
     1861 gatgatgata acttaaaaaa attcttgact tacctggagc agctgccaaa cacttacaaa
     1921 aatttctacg atacggtgct atataagacg cttttagcga ctggtttgcg tattcgtgag
     1981 tgtttagccc tcaaatggtc tgatattgac ttacaaaacg gtacactaga cgtaagtaag
     2041 actctaaact gtgaaaaaga ggtcactagc cctaagacta aatcaagtat tagggtaatt
     2101 gacttggata ataagacggt gctcatgctg cggctttaca aaaatagaca agcgcaagtt
     2161 ggtagagaaa taggcttgac ttatgaaaaa gtattttcta attcctttga cgaatataga
     2221 gacgctaggg cgcttaggtc aagacttgag aaacatttaa aactttctga ctgccctcgt
     2281 ttaacattcc acgctttccg tcatactcac gctagtatct tgctcaatgc cggactacct
     2341 tacaaagaga tacaaacacg gctcggtcat gcaaaacttt ctatgaccat ggatatttat
     2401 agccacttat ccaaagacaa caagaaaaat gctacttcat tttatgaaaa agccattgaa
     2461 aaattaaaaa gttcctaaaa agtccacaaa ttttataaat cggttatgaa tcaagccaca
     2521 aataccgcta tactagggaa tttaccccct agacttgaaa taaagcgcat ttctctatat
     2581 aataatactt atatagaaaa caccttttag aaagatacct atgcttccat atccattttc
     2641 ctttttttca agtatttggg ggtttcgtaa gcccctgtcc aaacgtttcg agctcaactg
     2701 gtttcaactt ctctttacca gtatcttcct tatcagcttg tctatggtac ccattgctat
     2761 ccaaaacagc tcccaggaga cctatccgct agaaactttt atcgataatg tctatgaacc
     2821 tctgacagat aaggttgtcc aggatctctc tgaacatgct acaattgtcg atggcacatt
     2881 aacttatact ggaacagtta gtcaagcctc ttctattgtg attggtccaa gtcaaatcaa
     2941 ggaattacct aaggacttgc aactgcattt cgatacaaat gagctagtca tcagcaagga
     3001 aagcaaggaa ctgacccgca tctcttaccg agccattcag actgagagtt tcaaaagcaa
     3061 agacagcttg acccaagcaa tttctaaaga ctggtaccaa caaaatcgtg tctatatcag
     3121 cctcttccta gttctcggtg cgagcttcct ctttggtttg aatttcttta tcgtctctct
     3181 aggagctagt cttctccttt atatcactaa gaaatcacgc ctcttttcat ttagaacctt
     3241 taaagagtgc tatcacttta tcttgaactg tttaggattg ccgactctga ttacacttat
     3301 tttgggatta tttggccaaa atatgacaac cctgattact gtacaaaata ttctttttgt
     3361 tctgtatctg gtcactatct tttataaaac acatttccgt gatccaaatt accataaata
     3421 ggagattttt atgcccgtta cgattaaaga cgtggccaag gctgctggtg tttcgccttc
     3481 aaccgtaacc cgtgttattc aaaataaatc aaccattagc gacgaaacaa aaaaacgcgt
     3541 tcgcaaagct atgaaggaac tcaactacca cccaaacctc aacgctcgta gcttggtaag
     3601 cagctatact caggttatcg gattagttct tcctgatgac tcagacgcct tctaccagaa
     3661 tcctttcttt ccatcggttc tacgtggcat ctctcaagtc gcatctgaaa accactatgc
     3721 cattcagata gcaacaggga aagatgagaa ggagcgtctc aacgctattt cacaaatggt
     3781 ctacggcaag cgtgtagatg ggctaatctt tctctatgcc caagaagaag accctctcgt
     3841 aaaactcgtc gcagaagaac agttcccctt ccttatctta ggtaaatctc tatctccttt
     3901 catcccactt gtcgacaacg acaatgttca agctggtttt gatgcgactg aatatttcat
     3961 caaaaaaggc tgcaaacgca ttgcctttat cggaggaagt aaaaagctct tcgtgaccaa
     4021 agaccgttta acaggctatg aacaggcgct taaacattac aaacttacca ctgacaacaa
     4081 tcgcatctac tttgccgacg agtttctgga agaaaagggc tataaattta gcaagcgatt
     4141 attcaagcac gatccacaaa ttgatgctat catcacaacc gatagcctcc tagctgaagg
     4201 tgtttgtaac tatattgcca aacaccagct ggatgtccct gttctcagct ttgactcggt
     4261 taatcccaag ctcaacttgg cagcctatgt cgatatcaat agtttagagc ttggtcgtgt
     4321 ttcccttgaa actattctcc agattattaa tgataataaa aacaataaac aaatttgtta
     4381 ccgtcaattg atcgcccaca aaattatcga aaaataagag actgggcaaa aagtcgttaa
     4441 aagcaaaaac gcatactatc aggtattgaa aaaacttgat actatgcgtt ttattgtggg
     4501 aagatttact tccttttcta ctgaaattga gtcttttccc aagatctttt tatactcaat
     4561 gaaaatcaaa gtgcaaacta ggaagctagc cgcaggttgc tcaaaacact gttttgaggt
     4621 tgtagatgaa actgacgaag tcagctcaaa acatggtttt gaggttgcag atagaactga
     4681 cgaagtcagt aacatatata cggcaaggtg aagctgacga ggtttgaaga gattttcgaa
     4741 gagtattaat cactaattat ctatctcaac aaatcttcct agaatatgaa cattttccga
     4801 gacagagaca aaggagcttg gatccacttg tgtcataatc tgtttaaatt cattaaactc
     4861 tgcacgtgta atgacagtga ttaaaactgc ctttctctcg tgattatagg ttccttctgc
     4921 atcgtggatc atggttgctc cgcggtgcaa ttttttatgg attttttcaa ttaccttctc
     4981 tggatgattt gtcacaatca tggcctgcat acgcttttgc ttagtaaaga ctgcgtctgt
     5041 cacacgacta gagacaaaga tggtaatcat agaataaaga gcgtatttcc aaccaaaggt
     5101 caaacctgct atcagcatga tagttccatt taccaagaaa gaaatactac cgacattctt
     5161 acccgttttc ttgcgaatag tcaggctgac gatatccgtc ccaccactgg agatattgtt
     5221 tcgaagagca aaaccaatcc ccaaacccat aacaacaccc ccaaaaaggg cattgataat
     5281 gggatcctcc gtcaaggttg ccacagggac aaactggata aagaaggaac tcatagatac
     5341 cgtgataaag gtaaagacgg tgaacttatg gccaatctga taccaagcta agaccatcaa
     5401 agggaagtta atggcgtaga agcttagcga aatcggaata tgaaaaccaa accagtgatt
     5461 actcaaggca gagataatct gtgccagacc tgttgcacca ctcgaataca catgccctgg
     5521 ttggaaaaag aaattaactg ctactgctga taaaaaacca tagaccagag aggccgaaat
     5581 cttctcatca tacttttctc gagagatact ttgtaagaca cgtaaaattt ttatctgata
     5641 agcaaagcgg cgcagataat agcgccaccg cttaattcgt tttgcttgtt tcatcttctt
     5701 ctacttgtaa gctgagttcc tctagttgtt tgagagcgac tgttgatgga gcttgtgtca
     5761 ttgggtcagt tgccttgttg ttcttaggaa aggcaatgac ttcacggata ttttcttctc
     5821 cagcaagcaa catgacaaaa cggtcaagcc cgatagccaa accaccgtgt ggtgggaaac
     5881 catagtccat ggcttcaaga aggaaaccaa actggtcatt ggcttcttca gctgagaaac
     5941 caagagcctt gaacatgcgt tcttgaaggt ctttttggtt gatacgaagg ctaccaccac
     6001 caagctcata accgttcaag acgatatcgt aagcaatggc acgaacctta gccaaatcac
     6061 cttctaattc gtgagcagtc tcttcctgtg gaagtgtgaa tggatggtgg gcgctcatgt
     6121 agcggccttc ttcttcagac cattcaaaca tcggccagtc aaccacccaa aggaagttga
     6181 acttatcatt atcaatcaag ccaagctctt tagcaatacg tccacgaagg gcacccagtg
     6241 ttgcattagc cacttcaagc gtatccgcca caaagagaac caagtcctta tcttcaagag
     6301 caagcgctgt tgtcaattct tcttggatac cagtcaagaa cttggcaact ggtccgttta
     6361 attctccatc aaccaccttg acccaagcaa gacctttggc accatactgt ttggctactt
     6421 ccgtcatctt gtcgatgtct ttacgtgaat agttgtccgc agctcctttg accacaatcg
     6481 cttttacagc aggtgcttct gaaaagactt taaagtctac acctttgacc acttctgtca
     6541 agtcctgaag caacatgtca aaacgagtat ctggcttgtc agaaccgtaa agagccatag
     6601 catcatcgta tttcatacga gggaacggta gcgttacttc gatgcctttt gtttccttca
     6661 tcacgcgcgc gatcaagcct tctgtaatat cttggatttc tttctcagta aggaaggacg
     6721 tttccaagtc gacctgagta aattcaggct ggcggtctcc acgcaagtcc tcgtcacgga
     6781 aacatttaac gatttggtag taacggtcaa aaccagcatt catcaagagc tgtttcgtga
     6841 tttgtggact ttgaggaaga gcgtaaaaat gccccttatt aacacgagac ggcactaaat
     6901 aatcacgcgc cccttcaggc gttgacttag aaaggaatgg tgtctccacg tcgataaact
     6961 ccaactcatc caagtagttg cggatagagt gggtcacctt ggcacgaagt ttaagatttt
     7021 ccaacatttc tggacgacga aggtcaaggt aacggtaacg caaacgtgta tcgtgatttg
     7081 cctcaatgcc atccttaatc tcaaatggtg ttgtcttagc tgtgttaagc acaataagag
     7141 ctgtcacgtt taactcaacc gcaccagttg gcaacttatc attggcttgc tcacgcgcag
     7201 cgacctgacc agtcacctca ataacaaatt cgctacgaag gctttcagct gttgccataa
     7261 cctctgcaga tactttttca gggttgataa ccaactgcat gattccttca cggtcacgaa
     7321 gatcgataaa gatcaaacca ccaaggtcac gacgacggcc aacccatcct ttcaaggtta
     7381 tttcttgtcc gatgtgttcc tcacgaacac gaccagcata catactacgt ttcattattt
     7441 ctctcctctt ttattctgtt actattttac cataaaagcg caggctcttc atgaaaatca
     7501 tcagaaaagt ttgccagtct ttaaaagtca ggtgaaagcc ctaaaaatta gcgctaatac
     7561 tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac cgtagtacgg ttactgactt
     7621 cgtcagtttc atctacaacc tcaaaaccat gttttgagct gacttcgtca gtttcatcta
     7681 caacctcaaa accatgtttt gagctgactt cgtcagtttc atctacaatc tcaaaaccat
     7741 gttttgagct gacttcgtca gtttcatcca caacctcaaa acagtgtttt gagcaacctg
     7801 cggttagctt cctagtttgc tctttgattt tcattgagta taatacaaaa atccgatgaa
     7861 cttcaccgga ctcttttatt ttgaattttt gcctgcttta cgcttttcag cgatttcggc
     7921 tgcctttcga ggcaagacaa tttccgttat gtaagccgtc ccaaaacgca gtacacctgc
     7981 aataggagca aagacaactg ctagatagtt atagaagaaa tcgcctttga aggcataagc
     8041 tagcgctcca atgatgaaaa atagaacgac tgcctgaatc actgctaata aaattactcg
     8101 tttcatgtga cctcctgact ctattatagc atgagaatca tcaaaaagcc gactaaatta
     8161 ttcaaagcgt gaagagaaat actgtagacc agaccttttc tgctaatgta agccaaaccc
     8221 aaactaaaac caaggctaaa atagacaaaa aattgttgca catcacctgg aaaatgaatc
     8281 aaggcaaata gaagactaga taccagaaga aaaatcaggg gtcgtttact attgtcctgc
     8341 ttaggaaaga gatagcgtgc taacatccct ctaaaaacaa tctcttccgt caaaggagca
     8401 aaaataacca cagcaaagaa tgagaaaagt ggttgagaca aggtcaagtc tgtcgctatt
     8461 tgctgattta ctgaaggatc atctggcaag aagaattgaa cgaccagaga taagaaccaa
     8521 accaaggcag gaagccaaat aaatcggtta aagccgctct tctcaatata aacaggagct
     8581 ttctgatacc atttgtaaat gccgtacaca tatactccag ccaagaccac atagagtaga
     8641 gtaacagcat agggtgaagc gcctaaagca agcgacgcag tcgcgagccc ctgaataaag
     8701 ccatagataa agaaaaagga tagaagggct agaagaaacc agccaaggtt tttaagtaat
     8761 ttcatagata actcctttat ttgaaataac gttttaccat aggtaactgc atcacattga
     8821 tataaacatg gatggctcct acaagcaaga aagctagtaa ctgaatctct cctgtcaaga
     8881 aagaaaggat aataagaaaa atatataagg ctggtaagac atattggtgt aattggaata
     8941 aaattcgaaa actctgttcc aaattagcct gacgctcccc ttcatcataa gaatttatat
     9001 agttcaagac atcctttggt gtagcgaaaa attccaaatc aaactgacga acaatcgcaa
     9061 tggttttaaa aagagatttt tgagcgacta agaataccac aaagagtaag aaagaaagga
     9121 aaaatgtttg agggtttgta tgcaatataa tcacctcact taatgaaata aaaatagcca
     9181 atggaatcgc tacacctgta atattaaaag caatggttcc aaactcaaga ttccgataca
     9241 tttgcacata ataggtttca ttcagatcgt catccatttc ctcttgatac aaggaatgaa
     9301 attttctgct tttctttaag aaattgaaag tcaaaaacat actaatgaaa cctatcagta
     9361 aacaaatagc tgatatccat ggcatcaagg cttttacatc taaaataatt tcgtgggatt
     9421 cgacacgtgc cttaaacatc cctacaaaca tgcccaagaa cccccccaag acaatagaca
     9481 tcaaaaataa caatctacgt ttctttttca tattcattct cctttttcac ttgctagatt
     9541 tttggatttc ttttcaatcc attcagttac tgggatgaga gcaaagtaga cccaaacaaa
     9601 ttggtcgctt tgatagggat taaaccagct taggtccatc ccaatcagta gaaatacgct
     9661 gactaataaa gctatgacca ctacataata aatcacttta tacttgttca tcactcgtcc
     9721 tcctccaaac gaaataccga ttcgactgtt tcgttgaaaa tttgagatat tttcagggca
     9781 atgataatgg atggggtgta ctcatcccgt tctagtaggc taatggtctg tctggaaacc
     9841 cctgccagtt tggctaggtc ggtttgattg agaccatcgc gagctcgaag ctcttttaga
     9901 cgatttttta gttgcatgtt acacacctac tctccgtcaa attcaacggt ttggatatct
     9961 tcaatacgtt gcaacttgaa tttttctttc ccctttttat caacactacg tagtttgacc
    10021 cattcctcgt caacatccac aacttcccag ttatctggcc caatatacac tcccgttata
    10081 attggttcct ttccaatcat ttcttgcaat aatctcgaca tttctgcgtt tcctttctct
    10141 tttcgctcaa gttttttgat tttattctct agtttcttga tttttttaga attattagaa
    10201 taaaagaaaa tcataaatag tataaatact agtacccaca ttataactcc tttctgcttc
    10261 ctatttctta acttgaattc attgtaacac atctttttct ttttgacaat tatagttgtc
    10321 aaaaagttta tgatttttgt cattttgcaa aagaaaaagg tcaggagtaa gttcctgacc
    10381 actttatcta tcattaatac tcttctaaaa tctcttcaaa ccacgtcagc ttcaccttgc
    10441 cgtaggtatg gttactgact tcgtcagttc tatctacaac ctcaaaacag tattttgagc
    10501 tgacttcgtc agtttcatct acaacctcaa aaccatgttt tgagctgact tcgtcagttc
    10561 tatccacaac ctcaaaacag tgttttgagc aacctgcggc tagcttccta gtttgctctt
    10621 tgattttcat tgagtataaa atcctagttt ttcaaagatt tctgagaagt tttggctgat
    10681 tgtctcaagt gacacttgca cttcttctcg ggtttggttg ttcttgaccg tcacttgtcc
    10741 gctttcgact tcgctctctc ctagggtgat gagggtctta gccgcaaaga catctgctga
    10801 cttgaactga gctttgagct tacggttgag gtaatcacgc tctgctttga aaccttgttg
    10861 gcgaagagct tggactagtt ctagggcttt gacatttgct ccatcaccca agactgcgat
    10921 atagacatct agggcgtttt cgatagggag ggccacgcct tgtttttcaa ggatgagaag
    10981 caggcgctct acaccaagcc caaaaccaaa tccagcagtt tcagggcctc caaagtaagc
    11041 aaccaaacca tcgtagcgac cacccgcaca gactgtcaag tcattgccct caatctctgt
    11101 gataaactcg aaaatggtgt ggttgtagta gtccagacca cgcaccatat tggtatcgat
    11161 gatgtaatct actccaagat tttccaacat ctgacgcaca gcatcaaaat gagcttggct
    11221 ttcttcatca agaaagtcca agatagacgg cgcattctct actgctacct tgtcttcttt
    11281 ttccttagag tccaagacac gaagaggatt ttcctccaag cgacgttggc tatccttaga
    11341 caaggtctcc ttgagcggtg tcaaatagtc aatcaaggct tggcggtagg ctgcacggct
    11401 ctcaggattt ccaagagtgt tgaggtgcaa tttgacacct tgaataccga tttccttcaa
    11461 aaaatgggct gccatagcga ttgtttccac atcggtagct ggattgctag agccaaaaca
    11521 ctcaacacca atctggtgga attggcgcaa gcgccctgcc tgtggacgct cataacggaa
    11581 cataggtccc atgtagtaga acttgcttgg cttttgcact tctggggcga agagtttatt
    11641 ttccacatag gaacggacaa cgggcgcagt tccttctgga cgaagggtaa tatggcggtc
    11701 acccttgtca tagaaatcgt acatttcctt ggttacaata tctgttgtat ctccgacaga
    11761 gcggctgata acctcgtaat gctcgaaaat aggcgtacgc acttctgcgt agttgtagcg
    11821 tttgaaaatt tcacgggcaa agccctcaac gtactgccac ttggcagatt cagcaggtaa
    11881 aatatcctgc gttccttttg gtttttgtaa tttcataggg aatcctcttt aaacttaata
    11941 gtcttatttt accataaata gagggattaa aacagtgaga aaaaaattag gatttagata
    12001 tcatttttga gattaagaat tgttaaaaaa atagctagca aggaaagacc aacaaatagc
    12061 atccaagtca actgcatgtt ccatacggct actagtgaaa aacaagctgt tcccacaggt
    12121 atggataagg taaacaatag atctaaaaaa ttactagtac gagctagaac ctctggagct
    12181 agatttttca tgagcatggc actaatcttt ggttgaactt taccagacac atacagagta
    12241 aagaagagaa atagcaaacc aagcacgact tgattgaata aattagccaa accaactaga
    12301 ctaagtccta cggtcgccca catcatcaat ctaggcaagg actgcttccc aaaataatca
    12361 ttgcccgtaa ggctactgat gatgactgct actaaaacac agaattgatt gataaatagt
    12421 gcctctgtat aagaaaaatt caagagagaa tggctcaaaa agaagatatt ataaattcca
    12481 cccaaagcgc cacccaagga attaataagc aagacagcaa agagcataaa accaaagttt
    12541 ttctgtccac ttttaagaaa aacgagacgt aaatttcggt aaattgttag gaactggtct
    12601 ttgatagaaa gcttctcatt ttttaagttt tcaccatcag cagatgacat tgacaggctc
    12661 aatttgcttt ttcctaaaaa gaggatagtg gctgatacta agaaaaagca ggcattgatt
    12721 cccgcaacga gagaaaaatt gttgaccgat agagctaaga gccagactcc gaaagcttga
    12781 ccaccaatag ctgaaatata ggtgatgaac tgtgaaaaag aataagcctc catcagatca
    12841 tcttcagcta ctttttcctt aataagaggc atacgcaggc cacctgcaaa atcactgatg
    12901 atatcactaa tgacattgat caaacacagg ctagaaaagg caaagagact agcttgctga
    12961 acaactaggg ctgctagaaa aaatagaacc gcctgaaaca aaccgctata gaccatccat
    13021 ttgaccttgt ccctcgtgta atctgcccga atccctgcaa aaactgtaaa gaaggtcgga
    13081 agaatcatga caatattcgc catagcaaca gcaaaagatg cttgtgacaa ggtcgatgca
    13141 tagacgataa agaccaggtt gaaaatcgaa gcaccaaaag cattgaagaa gcgtgataaa
    13201 gtcaaaattc tataagctgg atttctaaac aatagtttca tagataatct ccctccttgt
    13261 tctaagttct acatcaagaa tagctttagt atagcactta tgaatttgcg aaacgattct
    13321 tttgaatata tgcaacaaac aaaatgagca aaaaaacgat agaacttttg taatgacctg
    13381 agagagggca acagttctat cgcttcaaat tttttcttgg tttgcagata ctcaacattc
    13441 ggtcgtattt ctatagtatt cggcagattt attacagcca agcatctcaa aaatacggac
    13501 agcatcctcc atctttttct gaccttcctt gactctacct tgcttgctat caaggagacc
    13561 ttctgcccac agataaacaa ttcggaaata ggtctcattt tccttgtaga aatgctcttc
    13621 gataacacgt ttaaaataat aggcattggt aaattcttca cactcaatac tagctaaaaa
    13681 gccattcaat agtatagtat gaaaaaggtt tcgattgcca gacatttcca ttagaaaatc
    13741 agatttacgt accatttctc gtacatatct agtaaaaaga gaaacagata aaaatggaga
    13801 actgactgaa aataaattga gttcatagat tccccagatc tcggtagaaa acaaataatc
    13861 atgaaggact tttccttcct ctgctgttaa ctctaccctt tcatctatgc tcttcatata
    13921 agacttgata ataatggcat ttagaatatg tttctgtttg ttgtgagaat gggcatgctt
    13981 ttgatactcc ctgcgatata agtcctcaag aggtgctata ttctttggtt ccaagacatc
    14041 tgtaatttct tttctcaact cagaatctgt atcatactgg aaacctctcg ccagaaagag
    14101 gatctcctcc acactggcag atatattttc cagagcaaat agaaactttt ccaccgaaag
    14161 ctcactctga cctgtttcaa aacgggacaa catagacggc gaaaattgtc ctccggttgc
    14221 ttgtctcagt gagatatttc ttgactctcg taattgtcta aagacttttc caatctgctc
    14281 catagacttc cccttgattc cgtattttct tcattttatc atatttttca gaaaattcat
    14341 caaaaacttg ccaaattgtc agaattatga gaaaatagag gatatttatc acgtggaggg
    14401 actgctatga gagacgatat caaaatcaat gaccgtgctt tggccttgca agaccaaatc
    14461 atcgaaaaac tagagaaagt ttttgataca gatgtggaat tggatgttta caatctaggt
    14521 ctgatttatg aaatcaatct ggatgaaacg gggctctgca agattgtcat gaccttcacc
    14581 gatactgcct gtgattgcgc cgaaagcctg cctattgaaa tcgtggcagg tctgaaacaa
    14641 atcgagggta tcaaagatat caaggttgaa gttacctggt cgcctgcttg gaaaatcaca
    14701 cgaatcagtc gctatggccg tattgccctt ggactaccac ctcgttaagc aggccaatca
    14761 tttttaaaga tgaaaaaagc aagccaaaaa tggcttgctt tttgatactc aatgaaaatc
    14821 aaagggcaaa ctagaaaact agccgcaggt tgctcaaaac actgttttga agtt
//
LOCUS       DAVKGC010000042        14678 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKGC010000042 DAVKGC010000000
VERSION     DAVKGC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14678
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.042"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..349)
                     /locus_tag="VW049_001094"
     CDS             complement(<1..349)
                     /locus_tag="VW049_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9432232.1"
                     /translation="MKIPIQKFFNNEILAYLFFGIVTTLVAILLRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKI"
     regulatory      378..474
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            472..981
                     /locus_tag="VW049_001095"
     CDS             472..981
                     /locus_tag="VW049_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9432233.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     gene            1081..1153
                     /locus_tag="VW049_001096"
     tRNA            1081..1153
                     /locus_tag="VW049_001096"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1114..1116,aa:Thr,seq:ggt)
     gene            1496..2572
                     /locus_tag="VW049_001097"
     CDS             1496..2572
                     /locus_tag="VW049_001097"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9432234.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            2628..3563
                     /gene="dapA"
                     /locus_tag="VW049_001098"
     CDS             2628..3563
                     /gene="dapA"
                     /locus_tag="VW049_001098"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9432235.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(3610..4983)
                     /gene="mnmE"
                     /locus_tag="VW049_001099"
     CDS             complement(3610..4983)
                     /gene="mnmE"
                     /locus_tag="VW049_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9432236.1"
                     /translation="MITREFDTIAAISTSLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     SQVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(5119..5301)
                     /locus_tag="VW049_001100"
     CDS             complement(5119..5301)
                     /locus_tag="VW049_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9432237.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            5424..6014
                     /locus_tag="VW049_001101"
     CDS             5424..6014
                     /locus_tag="VW049_001101"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9432238.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            6011..7090
                     /gene="prfA"
                     /locus_tag="VW049_001102"
     CDS             6011..7090
                     /gene="prfA"
                     /locus_tag="VW049_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9432239.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            7090..7929
                     /gene="prmC"
                     /locus_tag="VW049_001103"
     CDS             7090..7929
                     /gene="prmC"
                     /locus_tag="VW049_001103"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9432240.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPAWSVTAADVSQEALELASENASNQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            7916..8515
                     /locus_tag="VW049_001104"
     CDS             7916..8515
                     /locus_tag="VW049_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9432241.1"
                     /translation="MDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNIASFEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            8517..8948
                     /locus_tag="VW049_001105"
     CDS             8517..8948
                     /locus_tag="VW049_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9432242.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIC"
     gene            9010..10266
                     /locus_tag="VW049_001106"
     CDS             9010..10266
                     /locus_tag="VW049_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9432243.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAALIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            10274..11248
                     /locus_tag="VW049_001107"
     CDS             10274..11248
                     /locus_tag="VW049_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9432244.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            11248..11862
                     /gene="pmp23"
                     /locus_tag="VW049_001108"
     CDS             11248..11862
                     /gene="pmp23"
                     /locus_tag="VW049_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9432245.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     QDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            11936..12910
                     /locus_tag="VW049_001109"
     CDS             11936..12910
                     /locus_tag="VW049_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9432246.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            12984..14606
                     /gene="rlmD"
                     /locus_tag="VW049_001110"
     CDS             12984..14606
                     /gene="rlmD"
                     /locus_tag="VW049_001110"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9432247.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIELREH
                     YNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
BASE COUNT         4459 a         2847 c         3155 g         4217 t
ORIGIN      
        1 ttatcttatc aatgttttca gttacgaatt gccctatgat gtgaggaaat tgggttacaa
       61 agagaaaagt taaaaacaag tctaacagaa aagtagaaag gcgagcaaga gaaaatttca
      121 ctaaacggat aagtctattt ttcttttttt gcttaaatac aattgtatca tttgtgataa
      181 aggcaaataa aataccgata atatttgcca aggccgtagc aaggagttct tgatgactta
      241 gctgataaat gactaatcgt aatagtatag caacaagagt agtcactata ccaaaaaaga
      301 gataggctaa gatttcattg ttaaaaaatt tttgaatagg aattttcatg tgttaagtat
      361 agcataaagc aggctattgt gctatactag tagggttgaa taaagcaacc cttggtgctt
      421 agcttctttc accaagcata ttacacgcgg gcaaccgcca aaggagaaaa gatgaaaaaa
      481 ttaactattc gtgatgttgc agatattgca atcgtcgctg ctatctacgt cgttttgact
      541 gtcacaccgc ccctaaatgc catcagctat ggtgcttatc agtttcgtat ttcagaaatg
      601 atgaacttta tggcttttta caatcctaag tacatcatcg gagttaccat cggttgtatg
      661 attgctaatt tctttagctt cggactgcta gatgtctttg ttggtggtgg ttcaacctta
      721 gtattcctca gtttaggtgt ttggcttttt gcaaaatata gcaaggatta cctctttaac
      781 ggattgattc gaaaagatca tttcttcttt tcaatcctct tttcaatttc catgtttact
      841 atcgctgcag agttaaatat tgtagcagaa ttaccattct tccttacttg gtttacaaca
      901 ggaatcggtg aatttgcttc attgattatt ggagcgatta ttattggtaa aattggtcgt
      961 cgaattgatt taagtaaata gaagaagata agctaggtag ttcttacctg gctttttcct
     1021 atggaaaatt tctaaggaaa cttgacaagt ttttctgaat atagtatact tcttaagtaa
     1081 gctgatttag ctcagttggc agagcgcatc catggtaagg atgaggtcgc cggttcaatc
     1141 ccggcaatta gcatgatatg tatagggaaa ctcttgaata cgagagtttt tttgttatac
     1201 tcgatcgaaa aaatttctta ttgacaaaat caagtatctc tagtaatata gaatatgcga
     1261 tgagtcgata ggtagtcttc ggactactat tgagcataag gaggtcataa cgcaggagcg
     1321 gaccttgatg agttgtgtga acctgctcat cacatgaaga tgcctcttag tccctagtct
     1381 agcgactggg atttttattt tccaaaaaat gatgaaaaag ctttgcaatt catggaaaaa
     1441 gtagtataat acttctatta tagaaatttt tagaaaattc cgaaagaggt tatttatggg
     1501 atatacagtt gctgtagtcg gcgcgacagg tgccgtcggt gctcagatga taaaaatgtt
     1561 ggaagaatca acacttccaa tcgataaaat ccgttacctt gcttctgcac gttcagcagg
     1621 taagtcattg aaatttaaag atcaagatat tacaattgaa gaaacgactg aaacagcttt
     1681 tgaaggagtt gatattgctc tcttttcagc aggtagttct acatcagcta agtatgcacc
     1741 atacgcagta aaagctggcg tggtagtagt agataataca tcttatttcc gtcaaaatcc
     1801 agatgttcct ttggttgttc cagaggtcaa tgctcatgca cttgatgctc acaacggaat
     1861 cattgcctgc cctaattgtt caacaatcca aatgatggtg gctcttgagc cggttcgcca
     1921 aaaatggggc ttggaccgta tcattgtttc aacttatcaa gccgtttcag gtgctggtat
     1981 gggagcaatt cttgagacac aacgtgaact tcgtgaagtc ttgaatgatg gtgtgaaacc
     2041 atgtgatttg catgcggaaa ttttgccttc aggtggtgac aagaaacatt atcctatcgc
     2101 ctttaacgct cttccacaaa ttgatgtttt cactgataat gattacacgt acgaagagat
     2161 gaagatgacc aaggaaacta agaaaattat ggaagatgat agcattgcag tatctgcaac
     2221 atgtgtgcgt attccagtct tgtcagctca ctctgagtct gtttatatcg aaacaaaaga
     2281 agtggctcca atcgaagaag taaaagcagc tatcgcagcc ttcccaggtg ctgttcttga
     2341 agatgatgta gctcatcaaa tctatcctca agctatcaat gcagttggtt cgcgtgatac
     2401 ctttgttggt cgtatccgta aagacttgga tgcagaaaaa ggaattcaca tgtgggttgt
     2461 ttcagataac cttctcaaag gtgctgcttg gaactcagtt cagattgctg aaactcttca
     2521 tgaacgtgga ttggttcgtc caacagccga attgaaattt gaattaaaat agtcatatcg
     2581 tttaggagtt cagatgaact ccttctttga aatagagagg tgttttcgtg tcttatcaag
     2641 atttaaaaga atgtaaaatc attacagcct ttattacccc cttccatgag gatggttcca
     2701 ttaactttga tgctattcca gccttgattg agcatttatt ggcccatcat acggatggaa
     2761 ttcttctcgc aggaacgact gctgagagtc caactttgac ccacgatgag gagttggagt
     2821 tgtttgcggc tgtacaaaag gttgtcaatg gacgcgttcc tttgattgcg ggtgtaggta
     2881 ctaatgatac gcgtgactct attgagtttg tcaaagaagt agcggaattt ggtggtttcg
     2941 cagctgggct tgctattgtt ccttactaca acaaaccttc tcaagaaggg atgtatcagc
     3001 actttaaggc tattgcagat gcttctgacc taccaattat tatctataac attccagggc
     3061 gtgtagttgt cgaattgact ccagaaacca tgcttcgctt ggctgaccat ccaaatatta
     3121 tcggtgtcaa agaatgtact agcttggcta atatggctta cttgattgag cacaagcctg
     3181 aagagttctt gatttataca ggtgaggatg gagatgcttt ccatgccatg aaccttgggg
     3241 cggatggggt tatttctgtt gcctctcata caaatgggga tgaaatgcac gagatgttta
     3301 ctgccattgc agaaagcgat atgaagaaag ccgcagcaat tcagcgtaaa ttcattccta
     3361 aggttaatgc tctcttctct tatccaagtc ctgctccagt taaggcaatt cttaactata
     3421 tgggatttga agctggaccc actcgtctac ctcttgttcc agcaccagaa gaagatgcca
     3481 aacgcattat caaggttgtc gtagatggcg actacgaagc aactaaggca actgtaacag
     3541 gggtcttaag accagattac taataaagac aataaaatcc atgaccgaat gaaggatcat
     3601 ggatttttct tattttccta aacagaattg gctaaagagt tgggtgatga gttcatctgg
     3661 agcagcatcc ccagtgattt ctccgaggat ttcccaagta cgagtcaagt caacttgaag
     3721 caaatcaact ggcatcccca gctcaagacc ttgattaacg gcttgtaggc tttcaactgc
     3781 tttttcaatc agggaaatgt gacgggcgtt tgacaagtaa gtagcatctt gctcgaccaa
     3841 gccagcattt tcaaagaaga ggttgttgat tcgctcttca atcttgtcga tgttttggtt
     3901 tttaaggact gaaatacgga taacgtcttc aggtagtttc gaagtttcaa tcgtttctgg
     3961 caggtcggtt ttattaagta gaataatgcg attggtatct tggctaattt caagaagttg
     4021 tctgtcttgc gcagtcagtg gttcactggc atttagcact agtagaacca agtcggcttc
     4081 cttgagggct tttttcgaac gctcaacacc gatttgttcc actatgtcat ctgtttcacg
     4141 aataccggct gtatcaatca atttgagagg tacaccattg atgttgacgt actcttcgat
     4201 gacatctcgt gttgtcccag cgatatctgt tacgatagcc ttgtcctcac gcaagaggtt
     4261 gttgagaagg cttgatttcc caacgttggg acgtccaatg atagccgttg aaattccttc
     4321 acgaaggatt ttaccacgac gtgctgtcct aaggagtttg gttagtaatt gctcaaactc
     4381 cattgtcttc tctcggacaa cagcagtagt ggcttcctca acatcgtcat actcaggata
     4441 gtcgatattg acctcaactt gggcaagtgt gttaaggatt tcttgacgga tattattgat
     4501 aagatcagaa agggaaccat caagttgttt gaccgcgatg ttcatggcct tgtctgtctt
     4561 ggctcggatg atatccatca ccgcctcggc ctgtgtcaaa tctacgcgac cgtttaaaaa
     4621 ggcacgtttg gtaaattcac caggttctgc caaccgagct ccttcacgga tagctagctg
     4681 gagaatctca ttggtcaccg caataccacc gtgggtgtta atctcgataa tatcctcacg
     4741 agtgaaggtc tttggagact tcatagcccc aaccataacc tcgtccatga ctttaccagt
     4801 cagaggatca ataatgtgac cgtagttgag agtgtggcta gcaacctgac tcaagtcttt
     4861 tcctttaaaa atcttttgcg caatagcaaa actgtctgtt ccgctcaggc ggacaatacc
     4921 aatagctcct tcacctagtg aagtagagat agcagcgatg gtatcaaatt cacgtgtaat
     4981 cataatgttt cctttaatta attgctcttt tcttggaatt ttccaagtct cttttcaaat
     5041 tgtaacaaat ttagactatt tcttcaatgg gtgctacttg gagattgaaa aagcctaagc
     5101 aattctgctt aagctagctt atttagtacg catttcccct tgtgggaagt aagttccttc
     5161 tggcatgtcg ttgatgatga catggacagc agattgaggt gctccagtgt tgcggacaac
     5221 tgcttccgtt acttccttag caagagcttt cttttgctcg agcgtgcgtc cttcaaataa
     5281 atcgatgcgt acaaatggca taatagcttc ctccactagt tttgatttct tccattttac
     5341 cacattttgc cgtttaaagc ttaagaaaat tatgatatac tagaatgtag caaaaattta
     5401 gaaatggacg tgaagcaaga aacatggcac agttgtacta tcgttatggg accatgaact
     5461 ctggtaaaac gattgagatt ctcaaagtgg cctataacta cgaggagcaa ggaaaaggtg
     5521 ttgtgattat gacctcggct ctggatacgc gtgacggtgt tggctatgtg tcgagtcgaa
     5581 ttggcatgaa acgccctgcc cttgctattg aggaaacaac ggatatcttt ggctatatcc
     5641 gagacctacc agaaaaacct tactgtgttt tggtcgatga agcccagttt ctcaaacgcc
     5701 accatgttta tgacctagct cgtgttgtgg acgagttaga catacccgtc atggcttttg
     5761 gcttgaaaaa tgactttcgc aatgaattgt tcgaaggttc caaatatctc ttgcttttag
     5821 cagacaagat tgacgaaatc aagaccatct gtcagtattg caagaaaaag gcgaccatgg
     5881 tgttgcgaac acaggatgga ctgcccgttt acgatggaga acagatccag attggtggta
     5941 atgaaaccta tatctcggtt tgccgtaaac attattttgc ccctgaaatc aataaggaga
     6001 atgaagaaaa atgaacatct atgatcaact acaagctgta gaagaccgtt atgaagaact
     6061 aggagaattg ctgagtgacc ctgatgtcgt ttcagacacc aagcgtttta tggagctttc
     6121 aaaagaagaa gcttccaatc gtgacaccgt aatagcctac cgtgagtata aacaagtcct
     6181 tcaaaatatc gtcgatgccg aagagatgat taaggaatca ggcggagatg cggacttgga
     6241 agaattggcc aagcaagaac tcaaagatgc caaggctgaa aaagaagaat atgaagaaaa
     6301 actgaaaatt ttgctccttc caaaggatcc aaacgatgac aagaatatca tccttgaaat
     6361 ccgtggagca gctggtggag acgaagcggc acttttcgct ggagatttgc taactatgta
     6421 ccaaaagtat gcggaagccc aaggttggcg ctttgaagtc atggaagcct ctatgaatgg
     6481 tgtcggtggt tttaaagaag tggttgctat ggtttcaggt cagtctgtat actctaagct
     6541 taagtatgaa tcaggtgccc accgtgtgca gcgtgtcccc gtgacagaaa gccaaggtcg
     6601 tgttcatacc tcaactgcca cagttcttgt catgcctgaa gtcgaagaag tagaatacga
     6661 tatcgatcca aaagatcttc gtgttgatat ttaccacgca tcaggtgctg gtggtcagaa
     6721 cgtcaataag gttgcgactg ccgtgcgtat cgttcacttg ccaaccaata tcaaggttga
     6781 gatgcaagaa gaacgtaccc agcagaaaaa ccgtgagaag gccatgaaaa tcatccgtgc
     6841 ccgtgttgct gaccactttg ctcagattgc tcaagatgag caggacgctg agcgtaagtc
     6901 gacaatcggt actggtgacc gttcagaacg gatccgaact tataacttcc cacaaaaccg
     6961 tgtcacagac caccgtatcg gcttgaccct ccaaaaacta gatacgattt tgtctggtaa
     7021 attggacgaa gttgtggatg ccttggtgct ttatgaccaa acacaaaaac tagaagaatt
     7081 aaacaaataa tgaaattagc tcaattattt tcaaattttg aagaagagtt gataagacaa
     7141 ggagaggaag cggaaagcct ctcttttgtc tatcgtagtc tgaaaaatct atcttttaca
     7201 gactttattt ttgccctcca gcaggaggtg acaacagagg aagaaaaaca atttgtagaa
     7261 gatatttacc agcaactagc agctcacaag ccagctcagt acatcattgg acaggcagat
     7321 ttttatggaa tgcatttaaa ggtggatgaa cgggttttga ttcctcgtcc agaaacagag
     7381 gagttagtgg agcttattct aactgaaaat cttgagacga atctctcagt tctggatatt
     7441 ggaacaggta gtggagctat tgctctcgct ctagcaaaaa atagaccagc ttggtcagtg
     7501 acagcagcag atgtttctca agaggcctta gagcttgcat cagagaatgc tagcaatcaa
     7561 aatcttaata tattttttaa aaaatctgat tgttttgcag aaatttctga aaaatatgat
     7621 ataattgtat ccaatccacc ctatatctct cgtgaagatg agtcagaggt aggcttgaat
     7681 gttttgcatt cggagcctca tctagctctc tttgcagacg aggatggcct agctatttac
     7741 tgtagaattg cggaagatgc aaaagactat ctcaaagatg gtggtaagat ttaccttgaa
     7801 attggataca agcaaggtca aagtgttccc gaacttttta ggaaacatct tcctgaaaaa
     7861 agagttcgaa cactcaagga ccaatttggt caaaacagga tggttgtggt tgatgatgga
     7921 caggattaga caagagttgg aaaatggtgg agctgtcgtt ctacctacag agacggttta
     7981 cggtcttttt gctaaggcct tagatgaaaa agctgtcgac catgtttacc aactcaaacg
     8041 tcgtcctaga gataaggcac tcaatctcaa tatcgcctct ttcgaggaca tcttgcactt
     8101 ttctaagaat cagccagctt atctacaaaa acttgtagag acctttttgc caggtccctt
     8161 gaccattatt ctcgaagcca atgaccgagt tccctattgg gtaaattctg accttgcaac
     8221 tattggattt cggatgccca gtcaccctat cacactggat ttaattcgag agacaggtcc
     8281 cttgattggg ccgtctgcca atatctcagg tcaggcaagt ggtgtaacct ttgaacaaat
     8341 tctgaaggat tttgaccaag aggttctggg tctggaagac gatgcttttc taactggaca
     8401 ggattcaact attgtggatt tgtctggaga caaggtgaaa atcttacgcc aaggcgcaat
     8461 taaacgagaa gatattcttg ctcggttgcc agagatttct tttgaggagg cttgaaatgc
     8521 taagagattt gcaagaaaca gatgtgaaag cgatatgtga catcaaccaa gaggctttgg
     8581 gttatacttt tagtccagag gaaacggcta gccaactagc tagactgtct caggattccc
     8641 atcatttcct acttggctat gaggatgcag ctaatcatgt cttacttgga tatgtccacg
     8701 ctgaagttta cgaatcactc tattccaaag caggatttaa tatcttagct ttagcagttt
     8761 cacctcaagc gcaaggtcaa ggtattggta aaagtttact acaagggttg gaagaagaag
     8821 caaaaagatg tggttatggg tttatccgct taaattctgc caatcatcgt ctgggtgctc
     8881 atgcatttta tgaaaaagtt ggctatactt gtgataaaat gcagaaacgg tttattcgca
     8941 tctgttagtt tgattttctt attgtaaaat caaactaatg gactagtcac acaataaagg
     9001 agaagaccta tgatttttga caaagatgat tttaaagcat atgatgctga tctctggaat
     9061 gctattgcca aagaagaaga acgccaacaa aataatatcg agttaattgc ttcggaaaac
     9121 gtagtttcca aggctgttat ggcagctcaa gggtctatct tgacaaataa atatgccgag
     9181 ggttacccag gacgccgtta ttatggtgga actgatgtag tagacgttgt agagactctt
     9241 gctattgaac gcgcaaaaga aattttcggt gctaaatttg ccaatgtcca accacattca
     9301 ggaagccaag ctaactgtgc ggcttacatg tccttgattg agccaggtga tacggttatg
     9361 ggaatggatt tggcatcagg tggtcatttg actcatgggg ctcctgttag cttctctggt
     9421 caaacctaca actttgtttc ttatagtgtg gatcctgaaa cagaactctt agactttgat
     9481 gctatcttga aacaagccca agaagtaaaa ccaaaactga ttgtagctgg tgcttcagca
     9541 tattctcaaa ttatcgattt ttcaaaattc cgtgaaatcg cagatgctgt cggtgcgaag
     9601 ctcatggtgg acatggccca tatcgctggt ttggttgcgg ctggccttca tccaagccca
     9661 gttccatacg ctcatatcac aacaacaacg acccacaaaa cccttcgtgg acctcgtggt
     9721 ggtttgattt tgaccaatga cgaagaactt gctaaaaaaa tcaattcagc tattttccca
     9781 ggtattcagg gcggtccttt agagcatgtt gtggcggcta aggcagtttc cttcaaagaa
     9841 gttttggatc cagccttcaa ggaatatgct gccaatgtaa ttaagaacag caaggctatg
     9901 gcagatgtct tcttgcaaga ccctgatttc cgtattattt caggtggaac tgaaaaccat
     9961 ctcttccttg ttgatgtgac taaggttgta gaaaacggaa aagttgctca aaacttgctg
    10021 gatgaagtca atattaccct aaataaaaat tcaatccctt acgaaacctt gtcaccattc
    10081 aagacaagtg ggattcgtat cggagcagca gctattactg cacgtggatt tggtgaagaa
    10141 gaaagtcgca aagtggctgc actcatcatt aaaaccctta agaattcaga aaatgaggct
    10201 gtattagaag aagtgagaag tgcagtcaaa gaattgacag atgccttccc attatacgag
    10261 gactaaaact tttatggaca tttatattaa gaaagccatt attcaccagt tcagtccgga
    10321 tgataccgag ctgttcttag cagataagtt tctcaatatt actccaaaaa tcgaagaata
    10381 cctacgtaaa aaaattgaac atgtgtattc agatgaagcc aagactggga ttttcgaaga
    10441 agaaaatccc ttcttcaatc atattacaga cgatttgttg gagacatcag taacgctggc
    10501 taatctttgg aaagaggagt ttagcatttc tgaaaatctc aagaccaatg acttgatttt
    10561 tgttcaattt tctaaagaag gtgtagaaca tttcgctttc ttgcgaattg ccctgcggga
    10621 gaccttgacc cacctcggag gagaagttga taatccaatc aagctgactc agaataacct
    10681 gcctggattt ggaacgggtg ctgacgaggc cttggtggtc aatcttcaga gtcgcaagta
    10741 tcacctgatt gaaaaacgaa tcaagtacaa cgggactttt ttgaactatt tttcagataa
    10801 tcttcttgct gtcgctccta agatttctcc taaaaaatct atcaaggaac tggaaaaaac
    10861 agcccagaga attgctgaat cttttaacac agatgatttt caatttcaat ccaaggtcaa
    10921 atcagctatt ttcaacaacc tagaagaaag caatgaattg tcacctgaga aattggctaa
    10981 tgaccttttt gacaacaatc tgacggctcg tttgagcttt attgaccaag tcaaagaagc
    11041 cgtaccagaa cctgttcaat ttgatgaaat tgatgccagt cgccaattaa agaaatttga
    11101 aaaccaaaaa ctctccttat caaatggaat tgagctcatc gttcccaata acgtctatca
    11161 agacgccgag tctgttgagt ttatccaaaa cgaaaatgga acctactcta tcttaatcaa
    11221 aaatatcgag gatatccaaa gtaaataatg tttaaacgaa ttcgaagagt gcttgtacta
    11281 gcagtcttcc tttttgctgg ctataaagct taccgcgttc atcaagatgt caaacaagtc
    11341 atgacctatc aacccatggt gcgagaaata ttgagtgaac aagacacccc agcaaacgaa
    11401 gagcttgtgc ttgctatgat ttatactgaa acaaaaggaa aagaaggcga tgttatgcag
    11461 tctagtgagt ctgcaagtgg ttccaccaac accatcaatg ataatgcctc tagcattcgg
    11521 caaggcattc aaactctgac aggcaatctc tatctggcgc agaagaaggg tgtagatatc
    11581 tggacagctg ttcaagccta taattttgga cctgcctata tcgattttat cgcccaaaat
    11641 ggcaaggaaa ataccctggc tctagccaaa cagtactctc gtgagactgt tgcccccttg
    11701 cttggtaata ggactggaaa gacttatagt tatattcacc ccatttccat ttttcacggt
    11761 gctgaactct atgtaaatgg aggaaactat tattattcta gacaggtacg acttaacctt
    11821 tacatcatca aatgtttcac tctcttttca acatctggct agaccaggtg tttttgttat
    11881 aagttttctt taagatagat atattactct aaagaagtaa aggaggcaat tccctatgag
    11941 aaagaaactc tttctgacta gcgctgcgat cttgtgggca gtaacagcta tgaatagcgt
    12001 tcatgcagca acagatgttc aaaaagttat cgatgaaacc tatgtccaac ctgaatatgt
    12061 cctaggttct tccctatctg aagaccaaaa aaatcaaact cttaaaaaac tgggctacaa
    12121 tgcctcaaca gataccaaag aactcaagac catgacacct gatgtttatt ctaaaatcat
    12181 gaatgtggcc aatgactcta gcttacagtt gtattcatca gccaagattc aaaagctagg
    12241 tgacaaatcg ccacttgagg tcaagattga aacaccagaa aatatcacta aggtgactca
    12301 ggatatgtac cgaaacgcag cagtaacgct gggtatggaa catgccaaaa tcactgtagc
    12361 agcccctatt ccggttacag gagaaagtgc cttagcaggg atttactatt cgctagaggc
    12421 taatggagcc aaggtgccac aagctaataa agatttggct caagaagagt tgaaggcttt
    12481 gtcagatatc aatgctgaaa acaaggacaa atcaggctat gatgctaata aattaaacgt
    12541 tgccctagct gatatcaagt caggacttgc caaagctaaa gaaagcaagg gaaatctgac
    12601 agaagaagat gtccgcaaga ttgttgaaga taccttaaaa aattacaaac ttgatcaggt
    12661 cataacagga aaccagatca atatcatcat caattttgcc ttgaatctct caaagagtga
    12721 tatcctcagc aatgcagatt tcactaaaac cctaaatgac cttaaacaaa gcatcgtatc
    12781 acaagctggc gacagtttta aaaatatcaa ccttaacttt gatgcggata aggcgctaga
    12841 ggacggtggt aacttcttaa gctccctctg gcaagccctt gtcaacttct tcaagagttt
    12901 tggttcttaa gaaatttcat ggtataatgg atggtaaccg taacgttgcc gtcttttttg
    12961 tcggccaata gaaagagaaa agaatgttaa agaaaaatga tattgtagaa gttgaaattg
    13021 ttgatttgac ccatgaaggg gcaggagttg ccaaggtaga tggtttggtc ttttttgtag
    13081 agaatgcttt accgagtgaa aaaattctca tgcgtgtcct caaggtcaat aaaaagattg
    13141 gctttggaaa agttgaaaaa taccttgtcc agtcaccaca ccgtaatcaa gatctagatt
    13201 tggcttacct gcgttcagga atcgcggatt taggacacct ttcttatcca gaacagctca
    13261 agtttaaaac caagcaagtc aaggacagtc tctacaagat tgctggaatt gcagatgtag
    13321 aagttgctga aacgcttggt atggaacatc cagtcaagta tcgcaataag gcgcaggtgc
    13381 ccgttcgtcg agtgaatggt gtcttggaaa caggattttt ccgtaagaat tcgcacaacc
    13441 tcatgcccct tgaagatttc tttatccagg atcctgtcat tgaccaagtc gtagtagctc
    13501 ttcgagacct gctccgtcgt tttgatttaa aaccttatga cgaaaaggaa cagtctggat
    13561 tgattcggaa tcttgtggtg cgtcgtggtc actattcagg acaaatcatg gtcgttttgg
    13621 tgacaactcg tccaaaagtt tttcgtgttg accaattgat tgaacaagtt atcaagcagt
    13681 tcccagagat tgtgtctgtc atgcaaaata tcaacgacca gaataccaat gcgatttttg
    13741 gtaaggagtg gcgcactctt tatggtcaag actatattac ggaccagatg ttgggaaatg
    13801 acttccaaat cgctggccca gccttttacc aagtcaatac tgaaatggcg gagaaactct
    13861 atcaaacagc cattgacttt gcagagttaa aaaaagatga cgtggttatt gatgcttatt
    13921 ctggtattgg aaccattggt ttatcagtcg ccaagcatgt caaagaagtc tacggtgttg
    13981 aactgattcc agaagcggtt gagaatagta aaaaaaatgc tcagctgaac aatatttcaa
    14041 acgcccacta tgtctgtgac acagctgaaa atgctatgaa gaattggctt aaagatggga
    14101 ttcaaccaac cgttatcttg gttgatcctc cacgcaaggg cttgacagaa agctttatca
    14161 aagcaagcgc ccaaacagga gccgatcgca tcgcctatat ctcctgcaat gtcgcaacca
    14221 tggcgcgtga tattaaacta taccaagagt tgggatatga attgaagaaa gtccagccgg
    14281 tggatctatt tcctcaaacg catcacgtcg agacggtagc acttttgtcc aaactcgatg
    14341 tcgataagca cataagtgtt gaaattgagc tggatttgac aagtgcggag agcaaagcaa
    14401 catatgctca aatcaaagaa tatgtttgga ataaatttga attaaaagtt tcgacattat
    14461 atattgcaca gataaaaaag aaatgtggaa tagaattacg agaacattac aacaagtcta
    14521 aaaaggataa acaaattatt ccacagtgta cacctgaaaa agaagaagcc atcatggatg
    14581 ctttgagaca cttcaaaatg atttaataga aaagaatgac agtatatgac tttctgcatt
    14641 tattacattc ctacttggta taggaacagc tattattc
//
LOCUS       DAVKGC010000043        14605 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKGC010000043 DAVKGC010000000
VERSION     DAVKGC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.043"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            773..2032
                     /locus_tag="VW049_001111"
     CDS             773..2032
                     /locus_tag="VW049_001111"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9432248.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQSIPMPYGKINSLRASYYFYGSFL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2048..2599
                     /locus_tag="VW049_001112"
     CDS             2048..2599
                     /locus_tag="VW049_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9432249.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            2592..3869
                     /locus_tag="VW049_001113"
     CDS             2592..3869
                     /locus_tag="VW049_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9432250.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATTINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            3885..4745
                     /locus_tag="VW049_001114"
     CDS             3885..4745
                     /locus_tag="VW049_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9432251.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKSGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVIGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            4968..5078
                     /locus_tag="VW049_001115"
                     /pseudo
     CDS             4968..5078
                     /locus_tag="VW049_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
     gene            5124..5917
                     /locus_tag="VW049_001116"
                     /pseudo
     CDS             5124..5917
                     /locus_tag="VW049_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            5966..6430
                     /locus_tag="VW049_001117"
     CDS             5966..6430
                     /locus_tag="VW049_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9432252.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            6587..7012
                     /gene="lacA"
                     /locus_tag="VW049_001118"
     CDS             6587..7012
                     /gene="lacA"
                     /locus_tag="VW049_001118"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9432253.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            7043..7558
                     /gene="lacB"
                     /locus_tag="VW049_001119"
     CDS             7043..7558
                     /gene="lacB"
                     /locus_tag="VW049_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9432254.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGDYHD"
     gene            7569..8498
                     /locus_tag="VW049_001120"
     CDS             7569..8498
                     /locus_tag="VW049_001120"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9432255.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            8500..9480
                     /gene="lacD"
                     /locus_tag="VW049_001121"
     CDS             8500..9480
                     /gene="lacD"
                     /locus_tag="VW049_001121"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9432256.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            9714..10550
                     /locus_tag="VW049_001122"
     CDS             9714..10550
                     /locus_tag="VW049_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9432257.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            10586..10903
                     /locus_tag="VW049_001123"
     CDS             10586..10903
                     /locus_tag="VW049_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9432258.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVQ"
     gene            10903..12594
                     /locus_tag="VW049_001124"
     CDS             10903..12594
                     /locus_tag="VW049_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9432259.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARYFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FNLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            12809..14215
                     /gene="lacG"
                     /locus_tag="VW049_001125"
     CDS             12809..14215
                     /gene="lacG"
                     /locus_tag="VW049_001125"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9432260.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDHENPADVRAAELEDIIHNKFILDATYLGHYSEKTLAGVEAILAANGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            14258..14467
                     /locus_tag="VW049_001126"
     CDS             14258..14467
                     /locus_tag="VW049_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9432261.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDDDLEEFKNLFLNFINPEE"
BASE COUNT         4536 a         2517 c         3165 g         4387 t
ORIGIN      
        1 agcggattaa aatggtctgg ggatagaccg ttttaagtct gacgctggaa ataagaattg
       61 tcagaagaag ggatagcgaa atcgtggctc tacgaacagg aacgtgataa taaggcgtat
      121 atagcggata agagggcctc aaactctaaa gtccaaaaag gtagtcgtaa cctatatgcg
      181 taaatcacga gagtaattga attcgtacta agattttcta ttttcactgt aaccttttaa
      241 cgtcctcgta tcttgtatac acgaggaaag atgtacgact tatcccgtga ggtctatcac
      301 tataaagaga aaacgacaga tagaagtgat cctgagtcac ggttatcttt aaaatagatt
      361 gtctgatagg acggtatgta taaaacgctt ctgtgaactg agagaagggg gagaagttct
      421 tgctaaaatt tagttgaaca gccgtattcc gatatttaga taagggatct agtcttagct
      481 cctactcagt tttaggggat aaaaaagggg caatagcgat tcgagaaaga ttatactctt
      541 cgaaaatctc ttcaaatcac gtcaatatcg ccttgccgta tgtgtaggat actgactacg
      601 tcagttctat ctacaacctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt
      661 tgatctttga ttttcattta aaattgaaaa ggatggaaaa aaggataaat ttatgatata
      721 ctttattttg aagaccttat tagaaatctt gaaagagtat tgaaaactta gaatgagaaa
      781 aattgttatc aatggtggat taccactgca aggtgaaatc actattagtg gtgctaaaaa
      841 tagtgtcgtt gccttaattc cagctattat cttggctgat gatgtggtga ctttggattg
      901 cgttccagac atttcggatg tagccagtct tgtcgaaatc atggaattga tgggagctac
      961 tgttaagcgt tatgacgatg tattggagat tgacccaaga ggtgttcaaa gtattccaat
     1021 gccttatggt aaaattaaca gtcttcgtgc atcttactat ttttatggga gcttcttagg
     1081 ccgttttggt gaagcgacag ttggtctacc gggaggatgt gatcttggtc ctcgtccgat
     1141 tgacttacac cttaaggcgt ttgaagctat gggtgcgact gctagctacg agggagataa
     1201 catgaagtta tctgctaaag atacaggact tcatggtgca agtatttaca tggatacggt
     1261 tagtgtggga gcaacgatta atacgatgat tgctgcggtt aaagcaaatg gtcgtactat
     1321 tattgaaaat gcagcccgtg aacctgagat tattgatgta gctactctct tgaataatat
     1381 gggtgcccat atccgtgggg caggaactaa tatcatcatt attgatggtg ttgaaagatt
     1441 acatgggaca cgtcatcagg tgattccaga ccgcattgaa gctggaacat atatatcttt
     1501 agctgctgca gttggtaaag gaattcgtat aaataatgtt ctttacgaac acctggaagg
     1561 gtttattgct aagttggaag aaatgggagt gagaatgact gtatctgaag acagcatttt
     1621 tgtcgaggaa cagtctaatt tgaaagcaat caatattaag acagctcctt acccaggctt
     1681 tgcaactgat ttgcaacaac cgcttacccc tcttttacta agagcgaatg gtcgtggtac
     1741 aattgtcgat acgatttacg aaaaacgtgt aaatcatgtt tttgaactag caaagatgga
     1801 tgcggatatt tcgacaacaa atggtcatat tttgtacacg ggtggacgtg atttacgtgg
     1861 ggccagtgtt aaagcgaccg acttaagagc tggggctgca ctagtcattg ctgggcttat
     1921 ggctgaaggt aaaactgaaa ttaccaatat cgagtttatc ttacgtggtt attctgatat
     1981 tatcgaaaaa ttacgtaatt taggagcgga tattagactt gttgaggatt aaaccgtaga
     2041 ggtgtttatg aatatttgga ccaaattagc aatgttttct ttttttgaaa cggatcgctt
     2101 gtatttgcgt cctttctttt ttagtgatag tcaggacttc cgcgagatag cttcaaatcc
     2161 agaaaatctt caatttattt tcccaacgca ggcaagtctg gaagaaagtc aatatgcact
     2221 ggccaattac tttatgaagt cccctttggg agtgtgggca atttgtgacc agaaaaatca
     2281 acaaatgatt ggttctatta aatttgagaa gttagatgaa atcaaaaaag aagctgagct
     2341 tggctatttt ttgagaaaag atgcttggtc gcaaggattt atgacagagg ttgttagaaa
     2401 aatttgtcag ctttcttttg aggaatttgg cttaaaacaa ttatctatca ttacccacct
     2461 tgaaaatgaa gctagccaaa gagttgctct taagtctgga tttagtttgt tccgtcagtt
     2521 taagggaagt gatcgttaca caagaaaaat gcgggattat cttgaatttc ggtatgtaaa
     2581 aggagagttc aatgagtaag catcaggaaa ttctaagcta tttggaggaa ttaccagtag
     2641 gtaaaagggt cagtgttcgt agcatttcga atcatctagg agttagtgat ggaacagcct
     2701 atcgggctat taaagaagct gaaaaccgtg gaattgtgga gacccgtcct agaagtggaa
     2761 caattcgtgt taaatcccag aaagttgcta tagagagatt aacgtttgct gaaattgcag
     2821 aagtgacttc ttctgaggtt ctggctgggc aagaaggttt agagagagaa tttagcaagt
     2881 tttcaattgg tgccatgact gaacaaaata tcttgtctta ccttcatgat ggggggctct
     2941 tgattgtcgg agaccgaacc cgtattcagt tgctagcctt ggaaaatgaa aatgcagttc
     3001 tggttacagg gggatttcag gttcatgatg atgtgcttaa actggccaat caaaaaggga
     3061 ttcctgttct aagaagtaag catgatacct ttaccgtcgc gaccacgatc aataaagcct
     3121 tgtcaaatgt ccaaatcaag actgatattc tgacagttga gaaactttat cgccctagtc
     3181 atgagtatgg ttttctgaga gagacagata cagttaaaga ttatttggac ttggttcgta
     3241 agaatcgtag cagccgtttc cctgttatca atcaacatca ggtcgttgtt ggcgttgtaa
     3301 ccatgagaga cgctggtgat aaatcaccaa gcacgacaat tgataaggtt atgtctcgta
     3361 gtctattttt ggttggatta tcgacaaata ttgccaatgt gagtcaacgg atgatcgcag
     3421 aagactttga aatggtacca gttgttcgaa gcaatcaaac tttgcttggc gttgtgacgc
     3481 gacgagatgt catggagaag atgagccgtt ctcaagtttc ggctctacca actttttctg
     3541 agcagattgg acaaaagctc tcttatcacc atgatgaagt agtcattaca gtggaaccct
     3601 ttatgctaga aaaaaatgga gttttggcta atggtgtatt ggcagaaatt ctgacccaca
     3661 tgacccaaga tttagttgtt aatagtggtc gcaatctcat tatcgagcag atgctgatct
     3721 actttttgca ggctgttcag atagatgata tattgcgcat tcaggcacgg attattcatc
     3781 atacgagacg gtcagctata attgattacg atatttatca tggtcaccag attgtttcaa
     3841 aagcaaatgt gactgttaaa attaattaga aactaggaga aaagatgata acattaaaat
     3901 cagctcgtga aatcgaagct atggacaagg ctggtgattt tctagcaagt attcatatag
     3961 gcttacgtga tttgattaag tcaggcgtag atatgtggga agttgaagaa tatgtccgcc
     4021 gtcgttgtaa agaagaaaat ttccttccac ttcagattgg ggttgacggt gccatgatgg
     4081 actatcctta tgctacctgt tgctctctta acgatgaagt ggctcacgct ttccctcgtc
     4141 attatatctt gaaagatggt gatttgctca aagttgatat ggttttggga ggtcccattg
     4201 ctaaatctga cctgaatgtc tcaaaattaa acttcaacaa tgttgaacaa atgaaaaaat
     4261 acactcagag ctattctggt ggtttagcag actcatgttg ggcttatgct gttggtacac
     4321 cgtccgaaga agtcaaaaac ttgatggatg taaccaaaga agctatgtac aagggtattg
     4381 agcaagctgt tattggaaat cgtatcggtg atatcggtgc ggctattcaa gaatacgctg
     4441 aaagtcgtgg ttacggtgta gtgcgtgatt tggttggtca tggtgttggc ccaactatgc
     4501 acgaagaacc aatggttcct aactatggta ttgcaggtcg tggactccgt cttcgtgaag
     4561 gaatggtctt aaccattgaa ccaatgatca atacaggcga ttgggaaatt gatacagata
     4621 tgaaaactgg ttgggcgcat aagaccattg acggtggatt gtcatgtcag tatgaacacc
     4681 aatttgtcat tacgaaagat ggacctgtta tcttgactag ccaaggtgaa gaaggaactt
     4741 attaataaaa agtgaaaaga ctactggaag tttattatga aaaaatccag tagatctttt
     4801 cataataaaa cgcattgtat caagtgttag gggctgatat catgcgtttt tctgctttta
     4861 agattttttc caactctgtt tgtaagcgca tcataacaaa gggtctagga ttcagggctc
     4921 tcctcctata tactattagt aaagtaaaac taagggagga tattttagtg tcgcagtcta
     4981 ttgttcctgt agagattcca caatattgtc gttttgattc taaaaagaga aatggaattc
     5041 tgtttaatgt tcgtattgcc aatcttaaat ttaccttttt tagattagac tccctgcgaa
     5101 acaaaacatg gtatagtagt tctatgaatg atgaagcaag taaacaacta actgatgcac
     5161 gatttaagcg ttttgttggt gttcagcgca cgacttttga agagatgtta gctgtattaa
     5221 aaacagctta tcaacttaaa cacgcaaaag gtggacgaaa acctaaatta agcctagaag
     5281 accttcttat ggctactctt caatatgtgc gagaatatcg aacttatgaa caaattgcgg
     5341 ctgattttgg tatccacgaa agcaacttta tccgtcggag ccaatgggtt gaagtaactc
     5401 ttgttcaaag tggtgttacg atttcaagaa ctcctctcag ctctgaggac acggtaatga
     5461 ttgatgcgac ggaagtacaa atcaatcgcc ctaaaaaaag aattagcgaa tgattctggt
     5521 aaaaagaaat gccacgctat gaaggctcaa gcgattgtca caagtcaagg gagaattgtt
     5581 tctttggata tcgctgtgaa ctatttgtta tgatatgaag ttgttcaaaa tgagtcgcag
     5641 aaatatcgga caagctggta aaatcttggc tgacagtggt tatcaagggc tcatgaagat
     5701 atatcctcaa gcacaaactc cacgtaaatc cagcaaactc aagccgctaa cagctgaaga
     5761 taaagcctgt aaccatgcgc tatccaagga gagaagcaag gttgagaaca tctttgccaa
     5821 agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat catcgtaaac gcttcggatt
     5881 acgaatgaat ttgattgctg gtattatcgg attctagttt tgcaggaagt ctaataaaga
     5941 aactagcaag gtcaggcctt actttattgg tggacatcca ggatttaatt gtcttctact
     6001 tgatgatgaa gtctatgaag attattatct agagtttgag aaagaagaga tttgctctgt
     6061 tccacgtcct ttcccagaaa caggtatgtt ggatttccaa gatagaagtc catggctaga
     6121 gggacaaaaa gaaatagatc ttagttatga tcttttcagc acagatgcag tgactttgga
     6181 tgaattgcaa tctcgaacaa ttgcccttcg ttctcttaaa catgataagg gattgaaagt
     6241 gcattttgca gaatttccaa acctcatcat ctggtcaact ttgaacaagg gacctttcat
     6301 tacctttgaa ccatggtctg gcttgtcaac attccttgaa gaaggagatc atttagaaga
     6361 taagaagaat gtttgtctct tagaagccaa tcaggttgaa gaattagggt ttgagataga
     6421 agttttataa aaaagtaaaa aataaataac aaaaacaaac acaaactgtt gacttcttca
     6481 aacaatagta gtatattatg tttgtgagga ctaattgact gttttttaaa gacagccaat
     6541 acactgctgg aatccagcat agaaaaaata aaaaggagta tacaatatgt ctattgttat
     6601 cggtgcagat gctgcaggtt tgagattgaa agaagttgtg aaagactttt tagaaaaaga
     6661 aaacttccac cttgtggatg ttacagctga aggtcaagac tttgttgatg tgactctcgc
     6721 tgttgctgca gaagtaaaca aagaagaaca aaaccttggt atcgtgattg atgcttatgg
     6781 agctggtcca tttatggttg caactaagat caaaggaatg gttgctgcag aagtatctga
     6841 cgaacgttca gcttatatga ctcgtggcca caacaactca cgtatgatca ctatgggggc
     6901 acaacttgtt ggtgatgaat tggctaaaaa catcgctaaa ggatttgtta atggtaaata
     6961 cgacggtggt cgtcaccaaa tccgggttga catgttgaac aaaatgggct aattagatta
     7021 tagtaagaaa ggtaagttaa aaatgagaat tgcaattgga tgtgaccaca tcgtaactga
     7081 tgaaaaaatg gcggtttcag aatttttgaa atcaaaagga tatgaagtca ttgactttgg
     7141 tacatatgac catacacgga ctcactaccc aatctttggt aaaaaagtag gggaagcagt
     7201 aactagtggt caagctgatc tcggggtatg tatctgtggt actggtgttg gtatcaacaa
     7261 cgctgtaaat aaagttccag gtgttcgttc tgccttggtt cgtgatatga caacagccct
     7321 ttatgctaaa gaacaattga acgccaacgt tatcggtttt ggtggtaaga ttactggtga
     7381 attgcttatg tgtgatatca tcgaagcttt cattcatgct gaatacaaac catctgaaga
     7441 aaacaaaaaa ttgattgcga aaattgaaca ccttgaaagt cacaatgctc aacaaacaga
     7501 cgcaaacttc ttcactgaat tccttgaaaa atgggatcgc ggagactacc acgactaaga
     7561 ggtaaactat gattttaaca gttacaatga acccatccat tgatatttcc tatcccttgg
     7621 atgagttgaa gattgatact gtcaatcgtg tggtggatgt aaccaaaacg gctggtggta
     7681 agggactcaa tgttacccga gtactttcag aatttggcga ttctgttctt gctactggtt
     7741 tagtgggtgg caaacttggt gagtttttgg ttgaacatat cgataatcaa gtaaagaaag
     7801 atttcttctc aattcaggga gaaactcgta actgtatcgc tattctccac ggagacaacc
     7861 aaacagaagt tcttgaaaaa ggtcctgaag tattggaaca ggaaggtcaa gactttttgg
     7921 aacatttcaa aaaactcttg gagtcagttg aagtagtggc catctcaggt agtctgccag
     7981 ctggccttcc agttgattac tatgcgagct tggtagaact tgctaatcaa gctggcaagc
     8041 ctgtagtctt ggactgctca ggtgcagcac ttcaggctgt tcttgaatca ccccataaac
     8101 caacagtcat caaaccaaat aatgaagaat tgtctcagct tcttggaaga gaagtttctg
     8161 aggatttgga tgaattaaaa gaagtacttc aagaaccttt gtttgcaggg attgaatgga
     8221 ttatcgtttc acttggtgcc aacggtactt ttgccaaaca tggtgacact ttctacaagg
     8281 tagatattcc tagaattcag gtggtaaatc ctgttggatc tggagactct actgtggcag
     8341 gaatttcttc aggacttctt cacaaagaat cggatgcaga attactcatc aaggcaaatg
     8401 tccttggtat gctcaatgct caagaaaaaa tgactggtca tgtcaacatg gccaactatc
     8461 aagttctata tgatcaatta atagtaaaag aggtataaaa tggctttaac agaacaaaaa
     8521 cgtgtacgct tagaaaaact ttctgatgaa aatggtatca tctcagctct tgcatttgac
     8581 caacgtggtg ctttgaaacg cctcatggct caacaccaaa cagaagaacc aactgtggcc
     8641 caaatggaag aacttaaagt cttggtagca gatgaattga ctaaatacgc ttcatctatg
     8701 cttcttgacc ctgagtatgg acttccagca actaaagctc ttgatgaaaa agctggtctt
     8761 ctccttgctt atgaaaaaac aggttatgac acaacaagca caaaacgctt gccagactgc
     8821 ttggatgtct ggtctgcaaa acgtattaaa gaagaaggtg cagatgcagt taaattcttg
     8881 ctttactatg atgtagatag ctcagacgaa ctcaatcaag aaaaacaagc ctacatcgaa
     8941 cgcatcggtt ctgagtgtgt ggctgaagat atcccattct tccttgaaat ccttgcttac
     9001 gatgaaaaaa ttgcggatgc aggttctgta gaatacgcga aagtaaaacc acacaaagtt
     9061 atcggcgcta tgaaagtctt ttcagaccca cgctttaaca ttgatgtctt gaaagttgaa
     9121 gttcctgtta acattaaata tgttgaaggc ttcgctgaag gtgaagtagt ttatacacgt
     9181 gaagaagcag cagccttctt caaagcgcaa gatgaagcaa cgaacttgcc atacatctac
     9241 ttgagtgctg gtgtatcagc taaactcttc caagatactc ttgtatttgc tcatgaatca
     9301 ggtgcaaact ttaacggagt tctttgtggc cgtgctacat gggcaggatc agttgaagct
     9361 tacatcaaag atggtgaagc agcagctcgc gaatggcttc gcacaactgg atttgaaaac
     9421 attgatgagc tcaataaagt tcttcaaaca acagcgactt catggaaaga acgtgtgtaa
     9481 gaaagtcctc ctagtttagg aacatgaatc taaaaaaatt taaaaaaagt tgtatgtaaa
     9541 ggtttacaaa ataacttact tgtgctatac ttaaatcaca agttaataca aggtgagtgt
     9601 tactaagtaa tattaggcat gatcacaggt gaattagaaa tcagctgatt ttctagttca
     9661 tttgtggtca ttttttgtac ttatatacct ttaagatata aaaggaggtt gacatgtatc
     9721 gaattctaaa tccaatgaat cacaatgtct cgcttgtcag aaatgataag ggagaagagg
     9781 tgattgtaat tggtaaggga attgcattcg gaaagaagaa gggggatttg attgctgaaa
     9841 atcaggttga gaaaatcttt cggatgaaga ccgaagagtc cagagaaaac tttatggctc
     9901 ttctcaaaga tgttccgctt gattttatca cagtgaccta tgaaatcatt gataagctat
     9961 caaagaaata tcattatccg attcaagagt atctctatgt aaccttgaca gatcatattt
    10021 actgttctta tcaagctcta actcaaggaa ggtacaagga tagtaatctg ccagatattt
    10081 ccgctaagta tcctgtcgct tttcaaatcg caaatgaagc ttttgaaatt taccgtcaga
    10141 agctagcaga tcattttcct gaggacgaaa ttattcggat tgcttatcat ttcattaatg
    10201 ctgaaggtga aaatgaagtg gaacttgtgg agtcgattga taagaggaaa gaaattctca
    10261 ggaatgttga agaagtttta acggactatg caattcaacg aactaaaaag aataaccatt
    10321 tctatgatcg ctttatgatc catttgaatt atttcttgga ttatttagac agatctagag
    10381 atgataacca atcacttctg gatatggaag atcatattaa acaatcctat ccaaaagcct
    10441 tcgagattgg ttccaagatc tatgatgtga ttacgcaaca tacgggtctt gatttgtata
    10501 aaagtgaacg agtttatcta gttctacata tccaacgttt attgtcataa aaatttattt
    10561 aaaactatat aaggagaatt ctatcatgaa tagagaagaa gtaacattgt taggttttga
    10621 aatcgtagcc tatgctggcg atgctcgttc aaaactattg gaagccttga aggctgctga
    10681 agctggtgat tttgaaaaag cggacgctct ggtagaggaa gctggtagct gtattgcaga
    10741 ggctcaccac gcgcaaacaa gtctattgac taaggaagct tcaggtgagg acttggctta
    10801 tagtgtaacc atgatgcatg gccaagacca cttaatgaca actatcttgt taaaagattt
    10861 gatgcatcat ttaattgaac tctacaagag aggagttcaa taatgaataa actaattgca
    10921 tttatcgaga aaggaaagcc tttctttgaa aaactatctc gtaatatcta tcttcgtgct
    10981 attcgtgatg gtttcattgc aggtatgcct gttattctct tctcaagtat ctttatcttg
    11041 attgcctttg taccaaactc atggggcttt aaatggtctg atgaagttgt agcctttctg
    11101 atgaaacctt atagctattc tatgggtatt ctggctctct tggtagctgg tacaacagct
    11161 aagtcattga ctgactcagt aaaccggagc atggaaaaaa ccaatcaaat caactatatg
    11221 tcaacattgt tggcagcaat tgttggtttg ttgatgttgg cagctgatcc tatcgaaagt
    11281 ggtctagcta ctggattctt ggggacaaaa ggtttgcttt cagccttcct tgctgccttt
    11341 gttactgtag ccatctataa ggtttgtgtt aagaacaacg tcactattcg tatgcctgac
    11401 gaagttccac caaatatctc acaagtcttt aaagatgtga ttccattcac tctatctgtt
    11461 gtttctcttt atgctcttga cttattagca cgttattttg ttggttctag tgtggcagaa
    11521 tcaatcggta aattcttcgc accactcttc tcagcagcag acggatacct tggtattacc
    11581 attatctttg gtgcctttgc cttcttctgg tttgttggga ttcatggtcc atctatcgtt
    11641 gaaccagcta tcgcagctat tacctatgcc aatgccgaag ttaacttgaa ccttctccaa
    11701 caagggatgc atgcagacaa gattcttact tctggtacac aaatgtttat cgttaccatg
    11761 ggtggtacag gtgcgacatt ggtcgttcca tttatgttca tgtggttgac aaaatcgaaa
    11821 cgtaaccgtg caatcggacg tgcttcagta gttcctacct tcttcggtgt aaatgaacca
    11881 atcttgtttg gtgcacctct tgttttgaat ccaatcttct tcattccatt tatctttgct
    11941 ccaattgcaa acgtatggat tttcaaattc tttattgaaa ctcttggaat gaactcattc
    12001 actgctaatc taccatggac aactccagct ccactaggtc tagttcttgg aactaacttc
    12061 caagtgctat cattcattct tgctgccctt ctaattgtgg ttgacgtagt tatttattac
    12121 ccattcctta aagtctatga tgaacaaatt cttgaagaag aacgttcagg taagtctaat
    12181 gatgaattga aagaaaaagt tgctgcaaac ttcaacactg caaaagcgga tgctattctt
    12241 gaaaaagcgg gtgtcgatgc agcacaaaat accatcactg aagaaacaaa tgtcctcgtt
    12301 ctctgtgctg gtggaggtac aagtggactc cttgcaaatg ctttgaataa ggcagcagca
    12361 gaatacaatg tccctgtgaa agcagcagca ggcggctatg gtgctcaccg tgaaatgtta
    12421 ccagagttta atctagttat ccttgcccct caagttgctt caaactttga agatatgaag
    12481 gcagaaacag ataagctcgg tattaaactt gctaagacag aaggcgctca atacatcaaa
    12541 ttaactcgtg atggaaaagg tgccctagca ttcgtacaag agcaattcga ttaaggctag
    12601 gaactgtgaa acagtctccc atcgttacgg aaatcgctat ggcgaatttc ctaatcgcct
    12661 tattaattcg tcggtaaaaa gatatcgttt ttacctcctc atgtcacaat tcagtgactt
    12721 ggtacaagaa gtgagatgga gaagcatggc tcactgactc ctctcctctc acttttactt
    12781 tatttaaatc aagaaatagg tgaaaaaaat gacaaaaaca cttccaaaag actttatttt
    12841 cggtggcgca acagctgctt atcaagcaga aggtgctaca catactgatg gaaaaggacc
    12901 agttgcttgg gataaatatc ttgaggataa ctactggtac actgccgaac cagctagtga
    12961 tttttacaat cgatatccag ttgacctcaa gctagcagaa gagtatggtg tcaatggtat
    13021 tcgaatttct attgcttggt cacgtatttt cccgactggt tacggccaag taaatgctaa
    13081 aggtgttgag ttttatcata atttatttgc agagtgtcac aaacgtcatg ttgagccttt
    13141 tgtaactctt catcactttg acacgccaga agctctccac tcaaatggag acttcttaaa
    13201 ccgtgaaaat atcgaacatt ttgtagacta cgctgctttc tgttttgaag aatttccaga
    13261 agtaaactat tggacaacct ttaatgaaat tggaccaatc ggtgatggtc aatatttggt
    13321 tgggaaattc cctccaggta tccagtacga ccttgccaaa gtctttcaat cacaccacaa
    13381 tatgatggtg tctcatgcac gcgcggtaaa attgtacaaa gagaaaggct ataaagggga
    13441 aattggtgtt gttcacgccc tgcctactaa atatcctcta gatcatgaaa atccagcaga
    13501 tgttcgtgca gctgagttgg aagatattat tcacaacaaa tttattttgg atgcaaccta
    13561 tctgggtcac tactctgaaa agactctggc aggggtagag gctatcttgg ctgccaatgg
    13621 tggtagcttg gatcttcgtg aagaagattt tacagcatta gaagctgcaa aagacttgaa
    13681 tgatttccta ggaatcaact actatatgag tgactggatg gaagcctttg atggagaaac
    13741 tgaaattatc cataatggta aaggtgaaaa aggaagctct aaataccaaa tcaaaggtat
    13801 tggtcgtcgt gtagctcctg actatgtacc acgcacggat tgggattgga ttatctaccc
    13861 tcaaggtttg tatgaccaaa tcatgcgcgt gaagaaagat tatcctaact ataagaaaat
    13921 ctacatcact gaaaatggtc ttggatataa agatgagttc gttgataaca ctgtttacga
    13981 tgatggacgt attgattacg tgaagcaaca cttggaggtt ttatctgatg cgattgcaga
    14041 tggagctaat gtaaaaggtt acttcatttg gtcattaatg gatgtcttct catggtcaaa
    14101 cggttatgag aaacgttatg gtctcttcta cgtagatttt gaaactcaag aacgttatcc
    14161 taagaaatca gctcactggt acaagaaagt agcggaaact cagattatag actagtagaa
    14221 ttagtcatta gatatagaat tttagtgagt caaaaagatg ttcaaagatt ttatccaatc
    14281 tatttatgaa aaagtttata ttataaattt cgaaaaatgc tctcaaatac cgtgtttgac
    14341 gagtgaagaa ttgaaaagtc ttggaaaatg gtatgtctcg actggtaaag aatggatttg
    14401 tcattcagat gatgatctgg aagaatttaa aaatctattt ttaaatttta tcaatcctga
    14461 agaatagaaa aacgcatagt atcaggtgtt gaaacacctg atactatgcg ttttattgtg
    14521 ggaagattta ctttttttct tctgaaattg agttgttacc caggctcttt cagtttatta
    14581 aggcttgatg actttaatgt gttta
//
LOCUS       DAVKGC010000044        14439 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKGC010000044 DAVKGC010000000
VERSION     DAVKGC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14439
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.044"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(90..989)
                     /locus_tag="VW049_001127"
     CDS             complement(90..989)
                     /locus_tag="VW049_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9432262.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(1061..1621)
                     /gene="rnmV"
                     /locus_tag="VW049_001128"
     CDS             complement(1061..1621)
                     /gene="rnmV"
                     /locus_tag="VW049_001128"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9432263.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(1621..2394)
                     /locus_tag="VW049_001129"
     CDS             complement(1621..2394)
                     /locus_tag="VW049_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9432264.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(2699..3363)
                     /locus_tag="VW049_001130"
                     /pseudo
     CDS             complement(2699..3363)
                     /locus_tag="VW049_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(3626..3760)
                     /gene="rpmH"
                     /locus_tag="VW049_001131"
     CDS             complement(3626..3760)
                     /gene="rpmH"
                     /locus_tag="VW049_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9432265.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(3905..5119)
                     /locus_tag="VW049_001132"
     CDS             complement(3905..5119)
                     /locus_tag="VW049_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9432266.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLGIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(5275..5568)
                     /locus_tag="VW049_001133"
     CDS             complement(5275..5568)
                     /locus_tag="VW049_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432267.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLIYPGFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            5730..6182
                     /locus_tag="VW049_001134"
     CDS             5730..6182
                     /locus_tag="VW049_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9432268.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIVHVIDI
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(6217..7605)
                     /locus_tag="VW049_001135"
     CDS             complement(6217..7605)
                     /locus_tag="VW049_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9432269.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            7671..8633
                     /locus_tag="VW049_001136"
     CDS             7671..8633
                     /locus_tag="VW049_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9432270.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAKKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(8662..9000)
                     /locus_tag="VW049_001137"
     CDS             complement(8662..9000)
                     /locus_tag="VW049_001137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432271.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDETNEVSSKHVFEVVDETDEVSSKHVFEVVDETDEVSSKHVFEVVDRTDE
                     VSNHTYGKAT"
     gene            complement(9064..10074)
                     /gene="ccpA"
                     /locus_tag="VW049_001138"
     CDS             complement(9064..10074)
                     /gene="ccpA"
                     /locus_tag="VW049_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9432272.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(10507..11106)
                     /locus_tag="VW049_001139"
     CDS             complement(10507..11106)
                     /locus_tag="VW049_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9432273.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLTLQPDVESVFQAKNGQEAIQLLEK
                     ESVDIVILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRSGYFERAVKAGVDAYVL
                     KERRIADLMHTLHTVLEGGKEYSPELMEVVMTHPNPLTEQEIAVLKGIAQGFSNQEIA
                     DKLYLSNGTVRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(11108..12205)
                     /locus_tag="VW049_001140"
     CDS             complement(11108..12205)
                     /locus_tag="VW049_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9432274.1"
                     /translation="MLERLKSIHYMFWASLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEYLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQIQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVEIANQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKVYLKLERTEKELILTVRDDGCGFVSISGDELHTVR
                     DRVLPFSGEVEVISWKQPTEVQVRLPYKERN"
     gene            complement(12205..12942)
                     /locus_tag="VW049_001141"
     CDS             complement(12205..12942)
                     /locus_tag="VW049_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9432275.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GEPANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(12944..13828)
                     /locus_tag="VW049_001142"
     CDS             complement(12944..13828)
                     /locus_tag="VW049_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432276.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(13845..14009)
                     /locus_tag="VW049_001143"
     CDS             complement(13845..14009)
                     /locus_tag="VW049_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432277.1"
                     /translation="MKKGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGME"
     gene            complement(14006..14212)
                     /locus_tag="VW049_001144"
     CDS             complement(14006..14212)
                     /locus_tag="VW049_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432278.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         4275 a         3335 c         2548 g         4281 t
ORIGIN      
        1 gcttatatct aaaaaaacag ttctaaagac gaactttaga actgttttga tacgaggaca
       61 aggacatttt tgtcactgat tcactatgtt tataaatatt ttttgacatc ctcttgccat
      121 tcctctgtca atttctttac tagaaatcca tctccaatta gcttcgcaaa tgttttagaa
      181 gactgataaa atgattcagc taaactaaaa tctctttcta ctcttaatgc ttgcttccac
      241 aaaaacataa atacaagagg tttcttttga taatcttgag ttttatccat aatttcttgt
      301 aaacacgata gataatcatt aaaacaatca gttactccta ccttttcaag acaacacatt
      361 cctgaaataa gagtatctct aattataaag taattctcta tttgagttac cttcttctgt
      421 tccaaaagaa agagcattag cttttcgatt tgatttataa atttggcaag atcttttcta
      481 atgagcattt gataaaaaaa caaacgaatc aacaacatat catttattga ataagcttct
      541 ttttctataa tctgaggcat atgttcctca agtattaact ctgcatattc aggccttcta
      601 gttctagaag tatccaaaca cgcctgcaat acttcacatg ctaattgttc ttctttagga
      661 agattatcaa agtatgtttt ataaatctct tccaaacacg cttccttctt atcgtactcc
      721 tcttctttac catagattgg ttcacgtaaa atctggtatt tcaattctaa ataagcagaa
      781 ggaagagctg aaaaatccgg cataaggcta taaactggaa ctcctagccg gcgagcaata
      841 taggctaact tgggcaaact cggttgcgaa gctccacttt caattcgcga cagttgacga
      901 actgtcagtt cttgctcatc tccacaaaaa tctggacgac taattccttt ttctatccga
      961 atctctttaa ttttttgacc aattaacatc agacgcctcc tcttctacct gcttcgaaaa
     1021 atactgtgaa acttaaaaaa ttgtaacggc tacatctttc ctactcataa gatttcatag
     1081 cttcttccac ttctgccaaa gtaaccccaa acaactctag gcgtttgagg agttgcttgc
     1141 cgttggaata gccgattcgg agagtctctc cgagatattc tctacgctta cggctgtctg
     1201 cccctgctag aaaaccaagg cgaatcaagt cgctacgact aatgtcaaac tgactctcat
     1261 gttcaaattg ttctgtcacc tgagctagag ccgttttcag gtcttcatag ctggcatgct
     1321 caattcccag agaacgcccc ttggtcttgg acttgggaac agcttcatct cgcttgagaa
     1381 aggcatgctg aactgttgga atggccgtca taatcatgcg ccgaatccgc tccccattaa
     1441 aatctgggtc tgtaaagaca atgactccat gacgttggtg tagacgctga attcgctcta
     1501 tatcctgatc attgatggca gaacctcgtg tctcataggt ctccacatcg aaataacgct
     1561 tgagattgac cgtatcatcg cgaccttcaa ccacgataac ttgagaaatt ttctctttca
     1621 ttacttgctg tccaatccaa aaattcgttc tgcatttgca gtcgttgcta ccgccagctc
     1681 ttctgtcgtc ataccacgca agtcagcgat aaagtcgacc acatagcgag tataggctgt
     1741 tttattttca cgaccacgct tgggtacagg tgctaagtaa ggcgcatctg tttccaccaa
     1801 aatcttgtcc aaaggtaact ctttagctgc ttcttggagg tcagttgcct tcttgaaagt
     1861 caccactcct gagaaggaaa tggtcatacc aagatccaca aacttctctg cccactcaag
     1921 cgtccctgaa aatgaatgca tgataccacc acgaggacca acgccctcac tcttgataat
     1981 ctcataggta tcttccagcg catcacgggt atggacaaca aaaggcaaat ccaagtcctt
     2041 agatagctga atctgacgac gaaaaacctg ctcctgcacc tctttgggcg ctgtcatcca
     2101 atggtagtct aagccaattt cacctagagc cacaaccttg ggatgtttta acttatccaa
     2161 caagtaagcc tcaacttcct ctgtataagt accagcttct gtaggatgcc aaccaatagt
     2221 cgcatagagc tgctcatact catctaccaa ctccaaggca cgctcaatcg tcggtttatc
     2281 aaaaccaaca atattcatct gtatcacacc catctcagca gccaaggcaa tttcttctgc
     2341 ctcacgacct gcaaattctt ctacattcaa gtgtgtatgt gtatcaaaaa tcatctcttc
     2401 taacctcgtt ttctatcttc tattatacca aaaaagagga ggagcaccta atttttcggt
     2461 ttcccctcct ctcttcaata gagagctatt ctgctatctt ttctatccga tattgcccat
     2521 ctcctattcc acagttagag acagaagaga ttggctacat tgatagaata ttcctattca
     2581 gctaaagtat gtgattatct tgggttctca cttttctcac ccatcactag gattgatgac
     2641 ttctcaaaat cccccttata atctaacttt aagtagagaa tagaattttc tagctgttct
     2701 attttctacg tctagtcttt cttcctagca aagcaaaacc acttgttgcc accactagac
     2761 caagactagc aagtgctgca cttgatgctg aacctgtgtt aggtaattgt ttgccagcag
     2821 gtgactggta ggtaacatct tgcattgttc ctgcagttgc ttgcggtgca cttgttgtct
     2881 taggagcagc attaggagcc actagtctct taggaactgt aaccatgaca atggcttgca
     2941 ctccaatcgt ttctgcttcc ttcacatctt ccatagtttt cgcattttca atctctttga
     3001 gagctgcttg tttttctgct tccactcttg ctaaaagttc tgctttttct ttatcagaaa
     3061 gcggtgcgcc tttgatttca tcctgcttgt ccttggctgc tttttcaatg gcattttagc
     3121 tgattctttt tcagtagcta attgcttaga agcttgtgat tgagctgagt tttgtgaacc
     3181 tgtgtgagct gatccacccg cctggctacc accgccttca ttcgcattag catctgagct
     3241 tccagtattg cctcccttat ctccacctgg tgtagtttct agcttatact ctggtgtagc
     3301 ttcttgagtt acagattcac ctttcgcaga ttgttgaact gtatcttttc ctagaatcgt
     3361 cactactgaa ccatctggga atgtgattgt tgctgtacca tctccaccta cttcaacttt
     3421 atagtaaaat aaaataataa caggacaaat cgatcaggac agtcaaatcg atttctaaca
     3481 atgttttaga aacagaggtg tactattcta gtttcaatct actataacat cgcttccgaa
     3541 tccatttgca tctactgaac ttttttacaa aaataaaaat cagtagactc gagttcctac
     3601 tgatttttga tagaattcgt ttggattatg cagccaaaac tttgcgtcct ttacgacgac
     3661 gagctgccaa tacgcgacga ccatttttag ttgacatacg gttacggaat ccgtgtttgc
     3721 gcgcacgacg aagtttactt ggttgataag tacgtttcac gatgaatacc tcctcataga
     3781 ttttgtattc gtttagccgg ctataaagtg atcagttact aaacatactt tactattcta
     3841 tctgattctt tcacttttgt caatagctct aggcaaatgt ttccagcttt ttcgaatgca
     3901 agccctatct acgatactgt ttcaagaaac gagtcatctt ttcttggatt tgggctaact
     3961 catcaacacg aggaaggtaa acgatacgga agtggtctgg ttcctgccag ttaaagcctc
     4021 gaccatgaac caagagaacc ttttcctgct tcaagaaatc aaggacaaac tgctcatcat
     4081 catcgatacg gtacatattg cggtcgattt ttgggaagat atagagcccc gccttgggtt
     4141 taacggcaga caaacctgga atatcttgaa tggcattata gatgaaattt ctttgctcgt
     4201 agattcgtcc accaggaaga agcaattcat cgactgattg gtgacccccc aaggaagttt
     4261 gtacgacttg ttgggccaaa acgttagagc aaaggcgcat attggacagc atattgagcc
     4321 cttcgatata gcccttaaca tgagtcttag ggccagacaa gaccatccaa cccacacgga
     4381 aacctgctat gcggtgggat tttgacagac cattcatgct gacacagaag acatctggtg
     4441 ccaagctcgc cacaggcgta tgcacatgtc cgtccattac catgcggtca tagatttcat
     4501 ccgcaaagat aatcaaatcg ttttgacggg caatctcaat aatccccagc aagagttcct
     4561 taggataaag ggctccagtt gggttatttg gattgataag gacaattgcc ttggtattgg
     4621 aagtgatttt tgacttgata tcgtcaatat ctgggtacca ttccacagct tcatcacaga
     4681 tatagtgaac ggcatttcct ccagctaggc tgacagccgc cgtccataga ggatagtctg
     4741 gcattggcac taagacctca tccccattgt ccaaaagccc ctgcatggac atgacaatca
     4801 actcactgac accatttcca aggtaaatat catcaatatc tacgtttggg aatttcttca
     4861 gctggcaata ctgcatgatg gccttacggg ctgagaaaat ccctttggag tcagaatagc
     4921 cttcactatc acgcgcattc ataatcaagt catgaatgac ctcgtctggc gctgtaaagc
     4981 caaattctgc tggatttcct gtattcagac gtaaaatctt ttctccattc gctcgcatcc
     5041 gcatggcttc ttccaataca ggaccacgga tatcataagc aacatgctct aacttactag
     5101 acttgttata ttctttcatc ttgtttcctc aattcatcag gatagtaaca ttataccact
     5161 agaaagagaa tgcgacaagt ttactgaaat gtgttactag tttcgtatag aagaaaaacc
     5221 ttgctaaagg cgaggttaag aacgtagaga tagcaggtat tttgcaaaga cacatcacat
     5281 accaaaagat aaacctagaa aaagcaagcc tagaaaggag tcataaagaa gattaaacat
     5341 gaggaaaagc ccccatatca tcaagtaatc ccaacgccca cttcgttttg caactagaaa
     5401 taagattaga tggtagagta catgaaagac taaaaatcca ataaaacctg gataaataag
     5461 cggtaccaaa ctctctaatt gccagatcat gatcacttct accaagactg aaactaggat
     5521 aattccataa tacaggaaat ttgatttttg aaaagagttt ttcatcattc tcccttcctt
     5581 cacttctttc ctgctatcct ttcttttatt ttatcatata tttatataga ttttaagccc
     5641 taatattctt ttgaaaaata ctttttttgc tttacttcta tagtgaaaag gtttacaatt
     5701 atagtaagaa aatttcagga ggtttcatta tggcacaacg ttaccaaaat atcatggtcg
     5761 caatcgatgg ttctaaggaa gcggacttgg cttttgtcaa gggagttcat tctgctctac
     5821 gaaacgacgc taaactcacc atcgtacatg tcattgacat acgcgctctc caaagcgtat
     5881 ccacctttga tgctgaagtt tacgaagaac tccaagtcga cgctgaaagt ctgatgaaag
     5941 agtacgaaaa acgtgctaaa gatgctgggg tagcagatgt tcatatcgtc attgaaatgg
     6001 gaaatccaaa gaccctgcta gcacgtacta ttccagatgc cgaggaagtg gacctcatcc
     6061 tcgttggcgc aactggtctc aacgcctttg aacgcctctt agtcggctct tcatctgaat
     6121 acatactccg ccatgctaag gtcgatttgc tggttgtgag agaacaagaa aaaaccttat
     6181 aatcacaaag aaaaggagcc cctagctcct ttttgtttac gatttatttc tctctttatg
     6241 gcgttcgtaa gccttgagct ggcgctgcag ttccttttta atagcaggtt ctggagcata
     6301 tttttcttcc caattatctg gttttaagat tttatgggtc actggatcaa aatgagcctt
     6361 gccatctgga aaaattttcc ccatattggc ctgatggaca atatcaaaaa tacgttctgg
     6421 gtccaccccc atcaagacaa aactgccgta ggtgaagtaa agcgtgtcaa tcaaggcatc
     6481 cacttgccct atcaaatctt gctgagcagg tgtcttcttg gctactttat ctgctgcctt
     6541 atcaagggcc tgatgaagtt gcgatacagc ttgaccaaaa tcttcttcag aaggactggc
     6601 tgctcgaaca aactccacca attcttctat tttaaaacca gccctatggg ttgcaccctc
     6661 taaatcccaa gctcgaggtt cttcttgggt tcgttcatcc atcatgtggt ggaaggtctt
     6721 gaccttattg aaatgatagt cacggctgac aaagactttt tctgaagaca aaacaccaaa
     6781 atgttccaag gccttgtgga tcccatcttg ttgattgcta gtggtaatgt gcttggcagc
     6841 ttccttgaca ctgctactac catttcccat agcgaccgac ataccgacgc cagccagcat
     6901 ttcaaggtca ttatctgagt caccaaaagc catgacttga ttgaggtcaa agccatattc
     6961 tttcccaact cggcgaatgc cttctaattt agaatttccc tgattgatga catccgatgc
     7021 aaaaggattg ctacgtgtca atttcaagtc ttcaaaatca gctgccgcct tctcagattc
     7081 ttctggtgtc atcagcatca aaacttggta gataggctga ttcatcaggt gaagcaggtc
     7141 ctcttccttt tggggaacaa ccttgctgac catgcgatta aaagactgac tcaccgtccg
     7201 agttaaaaca gagggaacga agcgactaat tcgttgggaa aaagaaccca gaccaaagga
     7261 catgatttta gaacccaaca tggcatcctt ggtccctaga gcaatctccg tgccctcttt
     7321 tttagcatag ctaattagat ggcgcaaatg taacttggaa atagggctcg tgaacaagac
     7381 tctgtcttta ctaaagatat actggccatt ataggttacc gcaaaatcca gatccaaatc
     7441 gtccatcaat tccttaacaa aaaaaggtcc tcgccctgtc gctacgccaa ctagtacccc
     7501 ttgttctttg acaatcttaa tcgcgtcctt agtggatttc aaaacactct tgcgattgtt
     7561 gaccaaggtt ccatcgatat caaaaaaaac agctttgact tccatcctat tccaatctcc
     7621 ccttttgtga tacaatgatt ataccacatt tcagaaagag ttagtaaatc atgcctaaga
     7681 aaatccttgt tttacatacg ggtggaacta tttccatgca ggccgatgct tctggcgctg
     7741 ttgtgacgag ttcagataat cccatgaacc atgtgtccaa cccacttgaa ggaatccaag
     7801 tccacgcctt ggactttttt aaccttccaa gtccccatat caaacccaaa catatgctgg
     7861 tcctctacca gaaaattaaa gaggaagcag ataactacga tggagtggta atcacacacg
     7921 gaaccgatac tttagaggaa acagcctatt tccttgatac catggaagtt ccccatatgc
     7981 ctatcgttct aacaggagcc atgcgtagct ccaatgagct cggtagtgat ggtgtttata
     8041 attacctaag tgctttacga gtggccagcg atgacagggc tgctgacaaa ggagttttgg
     8101 tcgttatgaa cgatgaaatc cacgctgcca agtatgtcac caaaacacat acgactaatg
     8161 tcagcacctt ccagactcca acacatggcc ccctcggtct catcatgaaa caggaaatcc
     8221 tctacttcaa aacagctgaa cctcgtgttc gctttgacct tgatcacata caaggtttag
     8281 tccctatcat ctcggcttat gctggtatga cagatgagct gattgatatg ctggatttag
     8341 aacacttgga cggtttgatt atccaagcct tcggagctgg taatattccc aaagaaacgg
     8401 ctaaaaaatt agaaagcctt ctgcaaaaag gaatcccagt cgctctggta tcacgatgct
     8461 ttaacggtat tgccgagcct gtttatgcct accagggtgg gggcgtacag ttgcaaaaag
     8521 caggcgtttt ctttgttaaa gaactcaacg cccaaaaagc ccgcttgaaa ctcctcatcg
     8581 ccctcaatgc cggactaaca ggacaggctt tgaaagacta tatggaaggc taatactctt
     8641 cgaaaatctc tgcaaaccac gtcacgtcgc cttaccgtat gtatggttac tgacttcgtc
     8701 agttctatct acaacctcaa aaacatgttt tgagctgact tcgtcagttt catctacaac
     8761 ctcaaaaaca tgttttgagc tgacttcgtc agtttcatct acaacctcaa aaacatgttt
     8821 tgagctgact tcgttagttt catctacaac ctcaaaaaca tgttttgagc tgacttcgtc
     8881 agttctatct gcaacctcaa agcagtgctt tgagctgact tcgtcagttc tatctacaac
     8941 ctcaaaacag tgtgttgcgc agcctttaac cagccgccta gtccgctcta tggtattcat
     9001 taagtcaaca tctcttgttt aagagcacaa aatcaggaaa tcttctcgat tccctgattt
     9061 tttctattta cgttttcgtg ttgagctacg ttctgtcaaa ccatgaggta agagaacttc
     9121 acgttcttcc aactcttcct tatgcataat cttggtcaac atacgcatac taatggcacc
     9181 aaggtcataa agaggttggg caatcgttgt caagtttgga cgggtaaagc gtgagatttg
     9241 tgaatcatca ctagtaataa tttcaaaatc ttctggcaca gaaacaccct tatcagccaa
     9301 accgttcaag actcctgctg ccaactcatc acctgtcaca actgctgcag ttgcatttga
     9361 tgaaatcaaa cgctctgcta aggcgtaacc atcatcatag ctatatttag attcaaatac
     9421 caaaccctca ctataagtga ttcctgcttt tttcaaggtt tccttgtagc caactaaacg
     9481 aaccttacca ttgatgtcat ccactagcgg accgctaacg aaagcaatac gctcattttc
     9541 tttagcaagg taactcactg catcaattgt tgcttgctta tagtcaatat tgacactggg
     9601 caactggtgc tcaacatcga cagttcctgc gagaacaatc ggagtacgtg aacgcgaaaa
     9661 ttctgagcga attttatctg tcaagtgata acccatatag ataatgccat ctacctgctt
     9721 tgaaaagagg gtattgacaa cagaaacttc tttctcgtta tcttcatcgc tattagctag
     9781 gacaatattg tacttgtaca tttctgcaat atcatcaatc cccttagcca aactcgaaaa
     9841 ataaccattg gtaatatttg gaatcacgac accgacagtg gttgtctttt tacttgcaag
     9901 accacgcgca actgcatttg gacgataatc caaacgatca attacctcta gcactttttt
     9961 acgggtattc tcttttacat ttttattgcc attgaccaca cggctgaccg tcgccatgga
    10021 aacacctgct tcacgagcga catcataaat ggttactgta tcatctgcat tcattccttt
    10081 tcctgtcctt tctatctcac acattctttt acaagtagag gtgctgattg aagctctata
    10141 tccacttaca aaagtgaaga tgtgaaaatg tgaaaatttc gttttcatat ttctacttat
    10201 tccattctat cactaattgt aaacactttc aagtgttttt tgaagattga ttgaaaaaat
    10261 ttcatagaaa acctaggttt agctccttgc taccacctta gactaaacaa aaaggaggaa
    10321 actaagccct cctaaagtta tagtaaaatg aaataagaac aggataaatc gatcaggaca
    10381 ggcaaatcga tttctaacaa tgttttagaa gtagaggtgt actattctag tttcaatcta
    10441 ctataggtat tgttccattc aatcccatca attttagcac ataatggttt tcgagatatg
    10501 atagtattac aaccaaccag attctttcgc gatattagct gcctctgttc gattacctgc
    10561 atctagtttc gaaagaatat tagtgacata gtttcggact gttccgttgg atagataaag
    10621 tttatctgca atttcttggt tagagaagcc ctgagcaatt ccctttaaaa ctgcgatttc
    10681 ttgctccgtt aatggattgg gatgcgtcat caccacttcc atcaattcag gcgaatactc
    10741 cttgcctcct tcgaggacag tgtgcaaggt atgcatgagg tctgcaatcc ttctttcttt
    10801 taatacataa gcatctactc cagccttgac cgcacgttca aaataccctg agcgcttgaa
    10861 ggtcgtcact acaaccacct ttgtttcaag cttttctgct cgtatccact ccaagacttc
    10921 aagacctgtc ttaacgggca tttctacatc aaggatgacg atatccacag actccttttc
    10981 taatagttgg attgcttctt gcccattctt ggcttgaaag acagactcta catccggttg
    11041 aagcgtgagc aactggcaca tggcatcccg caacatactt tgatcttctg caacaagtag
    11101 tttcatctta gtttctctcc ttataaggta gtcgaacctg aacttctgtc ggttgtttcc
    11161 aactaattac ctctacttct cccgaaaatg gaagaacacg atctcgaact gtatggagct
    11221 catccccgct tatagaaaca aagccacagc catcatctct cactgttaga atgagttctt
    11281 tctctgtccg ttctaatttt aagtagactt tagacgcttt agcatgtttg atgatattgg
    11341 tcactaattc aagcaaaatc atggaagccg ttgactccaa ttcctgagta aggctagctg
    11401 tatctagttg attagctatt tccacctcaa ttccagcaat ttctaacatc tttttcacag
    11461 tctctagttc ggatgtcaaa gttctagact taagattttc cacaatggtt cgcacttcac
    11521 gcattgattc tttgctaatt tgctgtattt ctcttaattc cttttccacc tgtggataag
    11581 cctgtatttg aaataactgc aaggctaaat ctgtcttgac actcaacata gcaaaggtat
    11641 gccccagact atcatgcaaa tcttgaccga tacgactgcg ttcattttca gcaagcaata
    11701 gatttatctg ggcattttgc ttagcctgag cttctttcaa atcctccaca atacgaatcc
    11761 gaaccaagcc aaaagtcatt aaatcgacaa aagcaagaat tacaagtaga tagaatagat
    11821 actcaacttc gattctctga aaaatcaaaa gttggcccac aacaaggact tgagcaagaa
    11881 gaaaagtcca gacatgtaaa gactttaaac cacctacgct gaaatgataa cttaagagat
    11941 tggataggaa aaagaaaaac cagatataat taacagcaac aaaggcagta ttcccaacta
    12001 cataagtcag catgaggccc cagaaaagcc aagataggcg ctggttctta gttattaaaa
    12061 cacccaaata cgtcactaca aatagaatat caaccaataa atgccaagca ggaagctccc
    12121 caatcactac aggtaggatg ggaaaaagca taaaaatcaa actggcccaa aacatatagt
    12181 gtatgctttt cagtctttca agcattaagc attctcctta tgaccttgaa ggtaaatagt
    12241 caaaccaaat aaaactgctg aaaaaacaag taaataaact gtggctgata gattgatgcc
    12301 accctcgttt aagaaggtct tgagcaactc catcaattga taggtcggga gacacttacc
    12361 aactgcttgc atccagtctg gaaataaaga aatgggcatc cagagtccgc ctaaaacagc
    12421 caatcctaga taaagaagat tgcccacaac agacatcagc tgactagttg gtaagagagt
    12481 caaggtcaag ccaagtgcta caaaagcaat actccccaca atcagcaaaa tcgcagctcc
    12541 aacccatttt actagaggca tgtctacacc tcttacaaaa tgtccaactg taaaaacaac
    12601 cagaattgag accaaataat caaccagcat acttgttatc tttgatagat aatattctac
    12661 catatttaca gggctatgac gcaatgtttt ctgccagttg ttcatcttat cggtatgtaa
    12721 aacagctgga aatgagagga tagttgtcga catcatggaa aaggcagtca tggagatgag
    12781 gtaatcacgc ataaaattcg cgggttcacc tggtgtgtcc tgatagatac cagaaaaaaa
    12841 taaatagaaa gccgtcggca tccctaccga caatagataa tagattaatt gtcgtttggt
    12901 caataaaaat tctatcttac taagtgctat ccatcgtttc atcttagtta tctcccttct
    12961 gcgtctcttc aaagattgta ttcaacaaac tacgattatt aacttcgatt tcttgcatcc
    13021 tacatcctgc ttgggctaat agttcccaga aagcatctgc ttctcgagtg actacttgta
    13081 aagaatcttg ctttagggtc cagttttcaa ccaaatttga ctgctctacg acttccttgt
    13141 aagctatagg aaggataaag tgcttttcta tttcctcgct acgcatagct agaggcgtcg
    13201 tatctcgaat caactctccc ttatttaaga gcaaaatgcg gtctgcagtg tgttccactt
    13261 cctcaatata atgggacgaa tagagaatag tgactccttg cgcttttaac tcctggacaa
    13321 tttcccaaaa acgttgacga gttgaggtat ccatggacgc agtaggctca tctaaaaaaa
    13381 caatctttgg tcgcccaatt aaggtcaaga tgaaagaaaa gagacgcttt tgaccgcctg
    13441 ataatttttc tgccaactgt tctttttgtt gcttgacaaa ctgcaatagt tgatcaattt
    13501 cctgattgct caaaggattt ggatagatac gttgaaagaa agcaatcaac tctttaactt
    13561 ttaatttctg aacgattaca ttttcttgag ggaggtaagc cctagtatag tctaacttag
    13621 aactcgtcac tggtaaacct tgaatagata cttgaccgct tgtgaccagt ttatctccaa
    13681 gcaaacaatc taagagtgtt gtctttccgg caccatttgg accaattaag gcaacacatt
    13741 caccatcgtt gatttcaaag gagatgttcc gcaaaatttc cttgtctttt attttcttac
    13801 tcaatttctc aactttaacc accgtcatga gactctcctt tcaatcactc cattcccata
    13861 aggaaaacga cgaaaatcat aaatccaaac cccaaagcac cacgaatgaa ttggcgaagc
    13921 aaggcttgat caaaccaatc agtaaacatt tccactaacc ataccaagag tgacaggccg
    13981 ataaagaaat agatgatccc tttcttcatt cctcaaactc ctttttcaca tctctgacta
    14041 atttcaaacc ttctctaaca agccaagaca tgattccaaa gccagcaaag accttccaag
    14101 gaaaatggta gaaatcctca tccaatcccg aaaacatgag ataggtcata actcctgcta
    14161 ctactaaact tactgcgata atcattttat ttctcatctc ttcttccccc atttcatact
    14221 acaattatag tcttttaaaa tcagaggaga cagaagcttc tgtcactaga aaatatgaca
    14281 aatgtcataa aaaattctgt tcaaaacaag caagatacac tatacaataa aacacaatca
    14341 gaaaaatcta aggcaacttc ctcaaaagag atatcaaacc caattcacac cataatgtaa
    14401 actaatactt atttaaaatc aaaaagagta gaaattttt
//
LOCUS       DAVKGC010000045        13951 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKGC010000045 DAVKGC010000000
VERSION     DAVKGC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13951
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.045"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            293..640
                     /locus_tag="VW049_001145"
     CDS             293..640
                     /locus_tag="VW049_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9432279.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(919..1755)
                     /locus_tag="VW049_001146"
     CDS             complement(919..1755)
                     /locus_tag="VW049_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9432280.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(1768..2397)
                     /locus_tag="VW049_001147"
     CDS             complement(1768..2397)
                     /locus_tag="VW049_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9432281.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(2407..3048)
                     /locus_tag="VW049_001148"
     CDS             complement(2407..3048)
                     /locus_tag="VW049_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9432282.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(3194..4423)
                     /locus_tag="VW049_001149"
     CDS             complement(3194..4423)
                     /locus_tag="VW049_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9432283.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSNLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(4413..5579)
                     /locus_tag="VW049_001150"
     CDS             complement(4413..5579)
                     /locus_tag="VW049_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9432284.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            5723..6736
                     /locus_tag="VW049_001151"
     CDS             5723..6736
                     /locus_tag="VW049_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9432285.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNNTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(6781..7200)
                     /locus_tag="VW049_001152"
     CDS             complement(6781..7200)
                     /locus_tag="VW049_001152"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9432286.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(7211..8617)
                     /gene="atpD"
                     /locus_tag="VW049_001153"
     CDS             complement(7211..8617)
                     /gene="atpD"
                     /locus_tag="VW049_001153"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9432287.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(8703..9581)
                     /locus_tag="VW049_001154"
     CDS             complement(8703..9581)
                     /locus_tag="VW049_001154"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9432288.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEVVAGASALE"
     gene            complement(9597..11102)
                     /locus_tag="VW049_001155"
     CDS             complement(9597..11102)
                     /locus_tag="VW049_001155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9432289.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(11117..11653)
                     /locus_tag="VW049_001156"
     CDS             complement(11117..11653)
                     /locus_tag="VW049_001156"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9432290.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(11653..12147)
                     /gene="atpF"
                     /locus_tag="VW049_001157"
     CDS             complement(11653..12147)
                     /gene="atpF"
                     /locus_tag="VW049_001157"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9432291.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(12161..12877)
                     /gene="atpB"
                     /locus_tag="VW049_001158"
     CDS             complement(12161..12877)
                     /gene="atpB"
                     /locus_tag="VW049_001158"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9432292.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(12912..13112)
                     /locus_tag="VW049_001159"
     CDS             complement(12912..13112)
                     /locus_tag="VW049_001159"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9432293.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(13187..>13951)
                     /locus_tag="VW049_001160"
     CDS             complement(13187..>13951)
                     /locus_tag="VW049_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9432294.1"
                     /translation="SVKKSSLTKELKEKILHYHNQKFSPEMMVMAKGVNVGISTIYYW
                     IHHGKLGLSKQDLPYPRKGKALKKQASTNFKPAGQSIEQRPEAINLRLENGHYEIDTV
                     LLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEF
                     NRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRRWLPKGTKKMTPKEVAFIEKWINN
                     YPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRFQ"
BASE COUNT         4258 a         3074 c         2492 g         4127 t
ORIGIN      
        1 tctagaatct tgatgtttgg gtctttaata tcgagcagtt ttgtgataaa atgtaattgt
       61 tccatatgaa tctttctaat gagttgtttt gtcgcttttc attataggtc atatgggact
      121 ttttttctac acaaaaatag gctccataat atctataagg gatttaccca ctacaaatat
      181 tatagagcct aaaagatgag accaaccaac ctcatctttt ttcgtatcaa atctaagcaa
      241 ttttagaaac tttatggcat actagactta tcaatgaaag cgaggtaatc tcatggaaca
      301 atttttagat aacatcaaag accttgaagt cactacagtt gtgcgtgcgc aagaagctct
      361 tgataaaaaa gaaactgcaa ccttctttat cggtcgcaaa acttgccctt actgccgtaa
      421 atttgcaggt acattgtcag gtgtcgtagc tgaaaccaaa gctcacattt acttcatcaa
      481 tagtgaagaa ccaagccaac tcaatgattt gcaagcattc cgctcacgct atggaatccc
      541 aactgtacca ggttttattc atattacaga tggacaaatc aacgtccgtt gcgactcttc
      601 aatgtcagca caagaaatca aagatttcgc aggattgtaa aaaatcagct aacaagtctt
      661 aaaatcacaa aaacgcataa tgtaaggtgc aaaaaccttg atattatgcg ttttattgtt
      721 cctaaaacag ttacatcagc atttcattac agaacatttt aattttggaa gtcaaatcaa
      781 tttttaacaa tattttagaa atcacagtgg gctattccat attcaattat ccagattcaa
      841 tcgctattat cgttttccta gattacaaaa aaaactacgt tttgctaatc aaaacgtagt
      901 ctcagccact attttatctt actgtccttc ttttacttct ttggttgcta catcttctcc
      961 aaaccatttt tggctgattt cttggaactt gccgtccttg taaagactag aaaaagcttc
     1021 atttatcttc ttaaccaagg ttgtatcttc cttacgggct ccaaccgcaa aagcttctgt
     1081 ttctagtcca actgtaaaga cattataatc gtttaaaaca ccttctgctt ctaaataata
     1141 gtttgcatag acacggtcaa tcaatagacc atcaattcga tcgtttttca aatcaatcaa
     1201 ggcttcatta aaggtttggt attgattcgc ttccttatta gcgacaatat tcttcaaaag
     1261 ttctggattt gcttcaaagt ctgcataacc agatgaacca gcttgagctc ctaatgtctt
     1321 tccagtcata tcctttgcag tcgtgatacc agatgatttc ttcgtaacca atacctgctc
     1381 attcttcata tatgagttac tgaaagccac cttttcacgg cgttcgtctg tagcggaata
     1441 gccattccaa atcagatcaa tcgttccttt tgtcaattca gcttctttca aatcccaatc
     1501 aatcggttgc caatttaccg tgattccgta tttttcaaaa acagctgtag ctaaatcaat
     1561 atcaaatcct gcataagaac catctttctg agcaaatccc attggaacaa aagtactatc
     1621 aaatccaata gtaatagact tgttagactg gtactttgac caattatctc cactagtttc
     1681 gctagaattt ttcccacaag ctactaagaa caaagcagtc atcagactga ctaatacaag
     1741 catccatttt ttcatccatt tttcctccta tttaggttct actttcaata atacatctgc
     1801 gatattttca gcaaactgca aatcatgggt aaccacaatc tgggtcatcc caagttccct
     1861 attttgcaag attagcttct ccacttccaa acgtaattct ggatccaggg cagaagttgg
     1921 ttcatcgtag ccaatgattt ctgggtcaat catcatagca cgcgccaaag ccactcgctg
     1981 cttttgccca ccagatagtg agaaaggata ggcctctgcg tgtcctccta gtcctaactg
     2041 ttccaagagt ccactcgcct tcttctcagc ctcttcctgc ttcattccca tggtcttcac
     2101 aggcgataaa gtcaaatttt ccagaactga tagatgagga aatagttgaa aatcttggaa
     2161 gacaaatccc agtaggttgc gcttctgcaa ttcatccagc tctaaaggtt gtccattata
     2221 aaagatttgc cctgaatcaa tggtttcaag acctgcaagc atacgtaaaa gagttgtctt
     2281 acctccacca gaaggtccaa cgatagccag gatttgcttt tcaggaatac ttagactgaa
     2341 attagacagg atttgtttgt ctccaaagac tttattgata tttcgtaatt ctaacatggc
     2401 agcctcctat ctataataac tatacttctt ctcaactttt ttggaaataa ttgtcacaat
     2461 cccaatcaaa atcaaataaa tggctcctgc caagaacata ggaactagac tagcatcgcg
     2521 gttagcagct gttcgactag ccaagataag gtctgaaatt ccgagagcat agaccaaaga
     2581 agtatccttg accaaactca taacttcatt aaagacacta ggaagaacaa tcttggtcac
     2641 ttggggcaag ataatatagc gcactctgtc aaaagggcta aactttaaga ccttggcagc
     2701 ctcatactgt cctcttggaa tagtgtcaat tcccccacgg aaaatttctg caaagtaagc
     2761 tgcatagttg agaacaaagg caataatagc tgcaggaagg cggtctaaac gaatcccaat
     2821 acttgggagc acataataga taaaaatcag ttgcaagagt aagggggttc ctcgcataac
     2881 ccagatgtaa atgttaatca gataatggag gggcttccaa tggacttgca aggcaaaggc
     2941 aatcaaaacg cccaagggaa tcgaaaatag caagaccagt gcaaagacct gtacagtcgt
     3001 gctcgcacca ttcagtaaac tcggtaatat ctcaaataaa taagacatac tgcacctcct
     3061 aaaagtaatt gttcctatta tagcatgaat aaacaagatt accaagtatt tttgtaaaaa
     3121 gattctacca tatacaaata aaaaccgtag ctatcaaaag agataaacta cagttctata
     3181 tgtttaaaca ttcttacaat ctctcaaaca gttcttgcat ttgcacatca tctggaacca
     3241 gttttaagta agtgtgagca tggacttttg cttcttcaaa atgtcccaat tcacgcaaga
     3301 gatagatata gtgttccaga aattctggat tgtccttcaa atctcctgtc aactcttgat
     3361 aatgctcata agcagtatcc aaatcgtcca tttcttgata agaacgagca atcatccact
     3421 tggtcaaaag attttctggc tcctcactct gcaagtctag aatatcctca taacgctcct
     3481 gctccagata aatagtggct aaacgaagca agatttcttc tgtatcctca gcgtcttctt
     3541 ttgcagtaag gagataattt tctgcaccac tagcatcatg caattcataa gaaaattgtg
     3601 aagcagctag caagaggcga gtttcaaagg gatttttctc taatccttgc ttagcgatac
     3661 gcagggcttc ttgaacttga tgttccttat gtaaagcctg actgtaccca tactcatagc
     3721 cttcaaagtc aggagaaatg gtatcaagct gcttaaagta gagggtggct ttttgatatt
     3781 cttcttgatc aaaataaaga ctggccaact caaaagctgt taagtcatcg tattctaact
     3841 ccagggcttt ttctaaaaac tcagtagccg tttcaaattt ccctaactga gcataggcaa
     3901 agccaattcg ttgataggtg gaaatgcccg tttgctcata aatcgagcga ttatctaact
     3961 gggcataggc ttgaatagcc gcttggtaat tttccaactc actatccaac tctgccaaac
     4021 ccaatatcaa gagagaatcc tctgagtagg tcaaggcctc caataatttc tcacgtgcca
     4081 catctgtcaa accttccagc tggtaaaggt ctgccttcaa aaccaaagac gagacatacc
     4141 agtcactgtc agcttggatt tcctcaagat aggtaaaggc ttcttctatt tgaccatcct
     4201 cgctagcaat tgcagctaga ttaagatgaa cctctggaaa atcctctaca attttcaggt
     4261 aaatttcctt ggcctgagga tagaaaccaa tcccttcaag ataagttgcc aactcataca
     4321 gaagattact tgaatcattt tctaaagctt tggcgaaata atgctcagcc ttagttaaat
     4381 cttgctcctc caaagcctgt aacatctgtt gactattgtt cactcttgac ttcctcaccc
     4441 tctaattcat atagaccact gactacctta taccattcga aaatggctga aatgacaacc
     4501 ttagcagagg cataaaccgg aataccaagt aaaactcccc agataccaaa catagatcct
     4561 gaagttaaca aaacaaagag aacattaata gggtggatgt ttaattgact tcccaaaatc
     4621 aatggagaga caaaacggcc ttcaatagtt tgttctacga taaagacaat cactactttc
     4681 aaaagcatga ctggaccagc aatcaaaccc aatactagag caggaagcat ggctagaaag
     4741 ctaccaagat aagggaccag atttaaaata ccagcagtaa cccccagcgt aaccgcatag
     4801 cgtagaccaa taatcttgaa gaagatgata aacattactg ctacaataat agccactgtc
     4861 acttgccctc gaacatagtt ggacaactgt tgattcacat ctgataaaac ttgtccaaca
     4921 ggttccttca attttcttgg aatgaattgg gtcaaatagt tacgcaagcc tttcccatca
     4981 cgcaagagat aaaagagcat gaaaggaacg ataatcaagg caacaatcac ttgagaagcc
     5041 ccgctaataa aggcactcac ccagttgact gcctgagatg aaaccttact tgccaaaact
     5101 gtagcctggc tagaaaaatt ggtcaaaact tgctctaatt gaggtctgaa atcatctggc
     5161 aggtgctggg ctaccaatcc attaacaatc ctatctatat cttctaagta aacaggaacg
     5221 tttcttgcaa aggtcaaaac ctgacgttgc agatttggaa tggcgactgc caagccccaa
     5281 atgataaaga gagcgatgat aacaaagaca atagtgatag ctataacacg attaacctta
     5341 tgcttctcca tccaatcaac aataggattc aacaaataat ataacaaacc agacaaaatg
     5401 actggcaaca tcacaactgc taaaaagtct aaaacaggtg aaaatagaaa actaatctta
     5461 cttaaaataa aaagattcag tcccaataat aaggttacta aaaataccgt aattgccttg
     5521 ttatctaaaa accacttgaa aaaccaagat aggctaaaat gtttctcttt ttgctccata
     5581 aatacttcct ttctattgtt ctctcattat accatttttg aaacaaaata actctttttt
     5641 aaagtgctta catctaattg gatatcttta atttacttgt ttttgtgata taatagtctt
     5701 atctttacac agaggtatag taatgaaaga aactgtttat tttggaactt atacacgtcg
     5761 tacttctcaa gggatttaca aggcagactt tgatacagaa actggtcagc tttcaaatct
     5821 agaacttttt gcagctgagc caagtccaac ctaccttgcc tttgaccagc accaacattt
     5881 atacactgtt ggtagccaag acgataaggg gggaattgca gcctatcaaa ctgacgggac
     5941 tgtgttaaat catgttgttg aagaaggagc tccccactgt tatgttgctg tcgatgaaaa
     6001 gcgtgatttg gtttacgcag ctaactatca caagggacaa gtccttgttt ataaacgcca
     6061 ggaagatggt agtcttctac ttagtgatat ggatcaacac agtggccaag gtccacatga
     6121 aaatcaagct tccccccatg ttcactatac agatttaaca cctgaccact atctagtgac
     6181 ctgcgacttg ggaactgacc aagtcatcac ctatgacctc gatcaagaag gaaaattatc
     6241 taagctctat acctatcaca gcaagccagg agcaggctca cgccatatca ttttccataa
     6301 ccactataaa atcgcttatc tcatttgtga actcaataat actatcgaag ttttaatcta
     6361 cgatggcgtt ggcgaatttg aacgtatgca ggtcatttca actttaccgg aagcttacga
     6421 aggctttaat ggaaccgctg ctattcatct ctctaaagac ggtaaatacc tctacgcttc
     6481 taaccgtggc catgattcta tcgcagtata taccatcctt gcggacggta gcttagagtt
     6541 attagaaatc gttccaacgc atggtcagac tccacgtgat tttgatttga cacccgacca
     6601 aaaatttctc attgttgtcc atcaagactc tgacaatgca actgtcttta aacgtaattg
     6661 tgacaatggt cgtctagcag aactctccaa cgacttccat gttcccgaag cagtctgcat
     6721 ccgttttgct ccttaaaaca ccataaaaaa tgaacttggt attttcaagt tcattttttc
     6781 ttatagtcta tttccgacat taatacggtt aatggcacgt tgcaaagcaa tcttagcacg
     6841 acgttcttgg tcaatcaaat gtttgtcttg tgcttcttca attgcacgtt ctgcacgaag
     6901 tttggcacgt tctgcacgac tgatatcgat atcacgagca cgttctgcag agtcagcgac
     6961 gattgtgatc atatcattgg caatttcaat aacgccaccg tttactgcaa tccagttcac
     7021 gtgatcttta tcatcgatac gttttacctt tacttcatca actgctaaaa ccgcaatcat
     7081 attttcatgt cgtggcaaga tccccatctc accatccaga gttcgaaccg atacatagct
     7141 ggcatggtga tcatagacga gaccatctgg tgtcacgatc tggacagtta actgagccat
     7201 agatcacctc ttaaaatccc attttttcag cttttgcaat cacatcttcg atagaaccta
     7261 caccacggaa ggcatcttct ggcaagtggt cgtatttacc atcaaggatt tccttaaagc
     7321 cacgtacagt ttcagcaact ggaacataag aacctggctg accagtaaat tgttccgcaa
     7381 cgttgaagtt ttgtgacaag aagaactgga tacgacgggc gcgagcaacc aaggtctttt
     7441 cttcatcaga aagctcatcc ataccaagga tagcaatgat atcttgcaat tcatggtaac
     7501 gttgaaggac acgttttact tcagcagcaa ctgcatagtg ctcttctcca acgatttcag
     7561 gtgccaaggc acgtgagctt gaagcaagtg ggtcaacggc tgggtagata cccaattgta
     7621 ccaacttacg ttccaagttt gttgttgaat ccaagtgagc gaaggctgtt gctggcgctg
     7681 ggtcagtata gtcatccgct ggcacataga tagcctggat agaggttaca gaacccttct
     7741 tggttgatgt gatacgttct tgcaattgac ccatttccgt agcaagtgtt ggttggtaac
     7801 caacggctga tggcatacga cccaaaaggg cagatacttc tgaaccagcc tgagtgaaac
     7861 ggaagatatt atcgataaag agaagcacgt cttggccttc cacatcacgg aagtattcag
     7921 cgattgtcaa accagtaagg gcaacacgca tacgtgctcc tggtggctca ttcatctgac
     7981 caaagaccat ggctgttttc tcgataacgc ctgattcttt catttcccag taaaggtcat
     8041 tcccctcacg agtacgttcc ccaacaccag taaatactga aataccaccg tgctcttggg
     8101 caatgttgtg aatcaattct tggattaaga cggttttacc aactccggca ccaccgaaaa
     8161 gtccaacttt accaccttta aggtaagggg caagaaggtc aataaccttg atccctgttt
     8221 caaggatttc agaagaggta gacaactcat caaaagttgg agctttttta tgaattggct
     8281 gacgctctgc gtcttctgta aaaggagctt ccaaatcaat ggtatctccc aaaacgttga
     8341 agacacgtcc caaagtttct ttacctactg gtacagagat tggacgacct gtgtccaata
     8401 cttccattcc acgagtcaac ccatctgttg attccatggc gatagtacga accataccat
     8461 ctcctaactc caaggctact tcaaggacga tttttgtttt tctttcgtca tttttgtaga
     8521 cgacaagtgc attgttaatc tcaggaagtt tttcccctgc tgcaaacaaa acgtctacaa
     8581 cgggaccgat aacctgagca attttacctg aactcatctc cttctcctat tctatataag
     8641 atactgtcgg ttcctagctc aactaggtcc taagttcatt ttcatacgag ctggactaga
     8701 gcctattcta aggcactagc acctgctacg acttctgtaa tttcttgtgt aatcgccgcc
     8761 tgtctggcac ggttatactg aattgtcaaa tcattgatga ctttcttagc attatctgtc
     8821 gctgtttgca tggctgtcat acccgcagca ttctcagctg tcttggcatc gataatggca
     8881 ccgtaaatca tactttctgc aaactgaggc aacaactgct ccagaatttc ttctcggctg
     8941 gtttccaatt caaaagtcaa gctgtactct tcatccgctt catttggatc caagtcaaca
     9001 atcggaagca tttgttccac acgcatttga ctggttagcg tattgacatg gtggttgtag
     9061 caaacataaa gctcatcaaa gagttcattt tggtacattt caacagtttt tgaaataatc
     9121 ttacgaactt gatcaaagct aggttggtct gccaagccac gtaattcata aagtggttga
     9181 ataccgcgag ccttaaagaa atcagctccc atcccaccga tacagatcat ttcaaaacct
     9241 ttaccgtctg ggtggtattc ttctttcaac tccataacag ctttcaaaat agaggaatta
     9301 taacctccaa ccaaaccgcg gtctgaagtg ataacgatat agcctgtctt cttcacagaa
     9361 cggctaatca acatcggatt agttgaagca ccagctccat taccatgaag gatatctgtc
     9421 aaaagtttac gcactttctg agcgtaaact tggaagttgc gagcagcttc ttcagaacga
     9481 cctagcttag cagccgatac catttgcatg gcattagtga tttgactcgt attttttgtt
     9541 gaggcgattt ttgttttaat atcatttaga gatactgcca tctgacacct ctattcttat
     9601 tggaagctag attgattgag aaactctgta atcgcagcat ccaagactgc ttcttctggc
     9661 aagtcttttg tatcacgaat ggtttccaaa atctctggat gttgagcatc aaagaaggta
     9721 tggaactctt cctcgaaacg aacaatatca tctactggaa cagtatccaa gaaaccatgt
     9781 gtcaaagcat aaagaatggt tacttgtttc tcaacaggta atggtttgtg aacaggttgt
     9841 ttcaagacct caacggtacg acgtccacgg ttcaacttag cctgtgttgc tgcgtccaag
     9901 tcagaaccaa acttagtaaa ggcttccaac tcacggtatg aagcaaggtc gatacgaagt
     9961 gtaccagcaa ccttcttcat ggctttgatt tgtgcagaac cacctacacg agatacagat
    10021 gaacccgcat cgatggctgg acgaatacct gcattgaaga ggccatcgcc aaggaagatt
    10081 tgtccatcag tgatagaaat cacgttggtt gcgatatagg ctgagatatc tcctgcttgt
    10141 gtctcgataa atggtagggc tgtaattgat ccaccaccaa gttcatcaga aactttagct
    10201 gagcgctcaa gcaaacggct gtggagatag aaaacatccc ctgggaaggc ttcacgacct
    10261 ggaggacgac gaagcaagag cgacagttca cgataagcta ccgcttgttt tgatagatca
    10321 tcgtatacaa tcaaaacatg cttaccttga tacataaatt cttccgccat agcaacccca
    10381 gcataaggag ctaggaagag caatggagat ggttgtgaag cagaggctgt cacaacgatt
    10441 gtgtagtcca aggcaccgta ctgacgaagt gtttctactt gcgtacgaac tgttgattct
    10501 ttttgtccaa tcgcgacgta gatacagatc atatcttgat ctttttggtt caagattgta
    10561 tcaatcgcaa tggttgtttt ccctgtctga cggtcaccga taatcaactc acgttgacca
    10621 cgaccaatcg gtacaagggc gtcaatagct ttcaaaccag tttgcaatgg ttctgaaaca
    10681 gacttacgtt gcataacacc aggagctggt gcttctactg gacgagtttt atcagtgtgg
    10741 atttctccaa gaccgtcaac tggacgacca agcggatcca caacacgacc aatcagactt
    10801 tcacctacag ggacttccat gattttccct gtacggcgga ttgtatcgcc ttcacggata
    10861 tctgtaaagt cacctaggat gataatacca acgtctgttg actccaagtt ttgagccata
    10921 ccataagagc cgttttcaaa attcaacaac tctccactca tgacattttc aaggccgtga
    10981 gcacgcgcga taccgtcccc gatataggtt acaacacctg tttcagtcac atcaaaattg
    11041 ggtttgaaat tttcaatttg ttgcttaatt aaagcgctga tttcttgtgc gttaattgcc
    11101 aaaagaacac cactttctat ttcaaatttt ctttaacaac tttaagttgt tgtttaatac
    11161 tcacatcaat tgtcttgtga ttggcaaaaa tgacaaaacc accaatgaga ctttcatcga
    11221 tttgttcttt tacactcctt actttcagag acattttttt ctcaatcaaa gggagcaaac
    11281 gagtcttctg ttcatcagtt agaggatgag cagacgtaat cgtcacttca aatcgatttg
    11341 tttctttttc aagtcggttc aagcaatcta caagcacatc ataaaaaaga tttgctctat
    11401 gattgtaggc cagaacctgg ataaagtttt gtaataaagg tgacacagaa tcttggaaaa
    11461 aagcaattgt tttttcctta tccgactcgt ctactgccac ttgctttaaa aaagaaggca
    11521 gacctgtttt ttcaacaact tgcttgattt gagtcaagtc tgaaaagata cggtcttctt
    11581 ctcctttttc aagtaccaat tggacaaaag gcatgctata tttttcaatt acctttactg
    11641 ttttcttgtc cattaagctt ctcctagctg atcgatatac tgatcaatga gtgctttatg
    11701 ggcatgactg tcaaggtttt gtgagatgat tttaccagct aagctgatgg tcaaatctgc
    11761 gacctcaccc ttaacactct gtaaagcttc tactttattt tgagcaattt cttgattggc
    11821 tttttctttt aagtgtcctg cttctagttt agcatctgct aagatattag ccttactttg
    11881 ctcagctgtt tcctttgcat tttcaatgat tgtcttagct tctttacggc taccagccaa
    11941 ttcatcttcg cgtttttgag ccaatacttc tgctttttga cgggcttctt cagctctgtc
    12001 aatatctgaa gcaatttttt cagctctttc ttcgaaaatg cctgtaatat tagaccatgc
    12061 aaatttttta atcaagacta gcaaaagaat aaaagagcca gtgattaaaa taaaattacc
    12121 aattaattca cctactgtta cgtgcatcag ttactccttt ctattcctct tcaatattaa
    12181 tcttattccc taaatacaca gatgtcaaaa gagtaaaaac ataagcttgg atgcaggaaa
    12241 taaagacaga aaatgcagtc caagccaaat tagctccaaa ggctactgga taccaataaa
    12301 tagcttggtg ggaaagaaga agtaacaaac tcgtcatgac ctctcctgca aagatattcc
    12361 caaaaatccg caaagccaaa gataagaagt ttgtaaattc ttccaagata ttcatcggta
    12421 tgacaaaaac aggactcata aaagatttta tacttttttt aaatccacga cgacgaacgc
    12481 tttctatatg tgtcaacaaa atgacaagaa aagataaggt taagtcatac tgtaaattag
    12541 cggttggcga actccaccag ttagtcccat cgatcgtttg aagctttgtc attaagccaa
    12601 ggttattggc aatcaccatg aaaaggaata aacaaaggaa aaagagtgag taatctttca
    12661 tgtagcgcga accaatgtta ggttctgtaa atccaataac aaagtcatag acatactcaa
    12721 gtacattttg ctttcctttg ggtttcaagg tcatattgcg gcttgcccaa taaataaaga
    12781 caaaaataac tcccacaatc aacaaagtca tggctaacat agtcagattg aagataacag
    12841 gaccaataga gatgattgga ttaatacttt cttccatcta aaatcctccc ttttcttctc
    12901 gttccatgta tttatttgat aataaatgag aagacaagag ttacaaagaa agttccttca
    12961 ataaaggcaa cacctaaaaa catcaaacta cgaaactcag aaagcatatc tggttggcgt
    13021 gctactgatt taaacagtcc attcatcaat aaaccttcac cgacagatac gcccatacag
    13081 gcaatacata agcctaaaaa tgttaaattc ataacaaatt ctcctttaaa ataaaattta
    13141 cttacttagt gtagtcctaa aatttaaatt tgtcaacttt taaccgctat tggaagcgtt
    13201 taataaaaat tatttccatt ttgctaaatt tcaagttcaa gttagccatc cagaagtctt
    13261 ctctgggtga cttgtagtcc aagcattttt taggatagtt gttaatccac ttttcgatga
    13321 atgcgacttc tttgggagtc attttcttgg ttcccttagg taaccatcta cgaatgagcc
    13381 tgttatgatt ctcattagtt cccctttccc aagaggcata ggggtgcgca taatagatgt
    13441 gctcctcaga aaatacatca aacaagcgat tgaattccgt tccattatct gccgtgatgg
    13501 aaagaatctt gtgttgtttt aagatgagtt ttagagcctg attgaccacc tcagcacttt
    13561 tatttggaat caatcggatg atctgatgtc tgctctttcg atccgtcaag acaagcaagc
    13621 agtagttttt cgctctcgta agtagaaccg tatcaatctc ataatgccca ttctccaagc
    13681 gaagattgat agcttcaggc cgctgttcga tggattgacc agcaggttta aagttggtgc
    13741 tagcctgttt cttaagcgct tttccttttc tagggtaagg caaatcctgc ttgcttaacc
    13801 ccaattttcc atgatgaatc caatagtaaa tggttgaaat tcccacgtta acccctttag
    13861 ccatcaccat catttcaggc gaaaattttt ggttatgata gtggagaatc ttttccttta
    13921 gttccttggt caagcttgat ttcttgaccg a
//
LOCUS       DAVKGC010000046        13368 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKGC010000046 DAVKGC010000000
VERSION     DAVKGC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13368
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.046"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            198..380
                     /locus_tag="VW049_001161"
     CDS             198..380
                     /locus_tag="VW049_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9432295.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDPYQGVNRLPAKL"
     gene            325..1174
                     /locus_tag="VW049_001162"
     CDS             join(325..738,740..1174)
                     /locus_tag="VW049_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9432296.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDTRFKRLVGVQRTTFKEML
                     AVLKTAYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEQIAAVFGIHESNFIRRSQ
                     WVEVTLVQSGVTISRTPLSSEDTVMIDATEVKINRPKKELANDSGKKKCHAMKAQAIV
                     TSQGRIVSLDITVNYCHDMKLFKMSRRNIGQAGKILADSGYQGPMKIYPQAQTPRKSS
                     KLKPLIAEDKAYNHALSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMNLIAGII
                     NHELGF"
     gene            complement(1246..1608)
                     /locus_tag="VW049_001163"
     CDS             complement(1246..1608)
                     /locus_tag="VW049_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432297.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHTFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFILDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1986..3866)
                     /locus_tag="VW049_001164"
     CDS             complement(1986..3866)
                     /locus_tag="VW049_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9432298.1"
                     /translation="MVRFTGLSSKQAQAIEVLKNHISLPDVEVAVTQSDQASISIKGE
                     GGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQKIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3860..4729)
                     /locus_tag="VW049_001165"
     CDS             complement(3860..4729)
                     /locus_tag="VW049_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9432299.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILNKTSIPTPESLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQIPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGQTDWDGRKIYQEAAAGNAICQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEENQW"
     gene            complement(4813..7458)
                     /locus_tag="VW049_001166"
     CDS             complement(4813..7458)
                     /locus_tag="VW049_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9432300.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTILVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTELIKKEEI"
     gene            complement(7549..8829)
                     /locus_tag="VW049_001167"
     CDS             complement(7549..8829)
                     /locus_tag="VW049_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9432301.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            9000..11084
                     /locus_tag="VW049_001168"
     CDS             9000..11084
                     /locus_tag="VW049_001168"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9432302.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QGGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEGGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKSSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIANCAKKLKNIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11124..12803)
                     /locus_tag="VW049_001169"
     CDS             complement(11124..12803)
                     /locus_tag="VW049_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9432303.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQYGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSMPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3743 a         3145 c         2690 g         3790 t
ORIGIN      
        1 aaattgtcat ctctccataa acacagtata tcatttttaa attttaagtc aaggaaaatt
       61 aacccacttt aatttctttt ttaacacggg ttaaaaaaga aattaactga gtaaaaactc
      121 tcgaaattca attcgctata ttatcactca acttgaacaa ataccagaac cacaaaaaac
      181 agatgtaaag gaaaaatatg gacttaaaat ttgaaggggt tgatttagaa tacaaaaaag
      241 ctaaaaacaa tcttccagag tctttctggg aaacctactc agcatttgca aatacaaatg
      301 gcgggaaaat aattctcggg atagatgaaa aaaacattga cccctaccaa ggagtcaata
      361 gactccctgc gaaattatga agcaagtaaa caattaactg atacacgatt taagcgtctt
      421 gttggtgttc agcgtaccac ttttaaagag atgttagctg tattaaaaac agcttatcaa
      481 cttaaacacg caaaaggtgg acgaaaacct aaattaagtc tagaagacct tcttatggcc
      541 actcttcaat atgtgcgaga atatcgaact tatgaacaaa ttgcggctgt ttttggtatc
      601 cacgaaagca actttatccg tcggagccaa tgggttgaag taactcttgt tcaaagtggt
      661 gttacgattt caagaactcc tctcagttct gaggacacgg taatgattga tgcgacggaa
      721 gtaaaaatca atcgccctaa aaaaagaatt agcgaatgat tctggtaaaa agaaatgcca
      781 cgctatgaag gctcaagcga ttgtcacaag tcaagggaga attgtttctt tggatatcac
      841 tgtgaactat tgtcatgata tgaagttgtt caaaatgagt cgcagaaata tcggacaagc
      901 tggtaaaatc ttggctgaca gtggttatca agggcccatg aagatatatc ctcaagcaca
      961 aactccacgt aaatccagca aactcaagcc gctaatagct gaagataaag cctataacca
     1021 tgcgctatct aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa
     1081 aatattttca acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgat
     1141 tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta ttgaaatact
     1201 aagcctacac acagactgat tccaattgtt ttttagattt caattttacc attcttcata
     1261 gtcaaaacca tcataatctg gatattgttc tggcgacgtt ccaaccgaac gtacgagata
     1321 agcttcttcg tcctctgtct cgatttctct cagcacttgg cattcaaaac gccattcatc
     1381 accgaagtct aaaataaact taaatttttg tttgacactt aggctatcca gatagacatt
     1441 ttctgtgtaa cgttcttcaa catcgtcact aacaaagcta agaaagtaag aatctgcatg
     1501 actccactca acattatcca tgaaaaatgt gtgagcatga tcatttgcaa aatcaaaact
     1561 ccataaaatc acatctgcta gttccgctaa cgtttttgtt tttggaatct atatatgtcg
     1621 gtagcaacct tttttaaatt tgacggaaat gatccaagct tctacagatg ctggcttttc
     1681 agcttctatt tcttgtttta ctaattttaa gtgtagcata atacctccaa ttgctcatcg
     1741 atagaattgc tcttaggaaa cattcacttt ctgctatttt atcataagtt tgtctaactg
     1801 tagcatttga aaccagataa ttcttctgtt tcacgcgaaa caccctgcgc caagcaaggt
     1861 gttcttgttt ttatactcaa tgaaaatcaa agagcaaact aggaagctag ctgcaggctg
     1921 ctcaaaacac tgttttgagg ttgtagatgg aagctgacgt ggtttgaaga gattttcgaa
     1981 gagtattaag tcgtataaat cgtcgacgct gtcgcaatgg tatgccactg gttggctgta
     2041 gttgctgcga aatccttgtc tgcgtagaag tcagtaaatg gtaggatttc aacttccagc
     2101 tcgtctatgc ggtcaagctg accagccaga taaacctcga tacggctttc tgctcgtttg
     2161 atgcgttgca agagtccgcc catacggatg tcaactgtat ccaaaccaaa gaccttgttt
     2221 tctttcagcc attggtgact aaagagggca tggaagtctt caatttggct tctaagttct
     2281 ggtaattctt gtctggcgat ttgttgtaaa ctttctttat catccgcttg gtaggcctga
     2341 cgaatgcgtc gtcccacatc tactttgcta cttaaaatag cattcaactg ggcctgagtt
     2401 tcaaagagat aggcatagtt tccagctttt tctttaatgt tagcaagcgt ctcagcagcc
     2461 tgagcgaagt gcggtttgtc ctgttcaggt gtcatgtgtt gatcaagaat cggacaaaga
     2521 atatcctgat aaaaaacata gcggttggga ttgataccgc tgagattgcc tggtaggtct
     2581 ggtaagaggt tggcaaggtc aatctgcata aaatcctcaa ccgttagacc agtattggtc
     2641 ttgaagtgcg cagacaaact atctaggtca ttgcgatagc tgagttctgc ccagatttgc
     2701 aagcttggta ggatagagaa ctgggcagtt tcaccaccat tgtctcccca accagttacg
     2761 atgacttctt taatctgatt ggcacggcag gctttattag cctcgatagc cactagacgg
     2821 ctaaaatgat tgtgaggtgt aaagccaatc cacttccaag ctccccctgc aaatgcaagg
     2881 tcatggctaa tcttgtgatg attgcggaaa ttacggttgt atttttcctc gctatcctga
     2941 taataatccc agtaaaccag agtcacacgg tctttgagac ggtctaggta gacacgagtt
     3001 tcctctggaa tttccacatc acggtcgtac tggccatccg ctgacatgag tttgaagaac
     3061 atatcactcc acatctggca gtggaaacca tatttgtcag caatatccag cacgcgctcc
     3121 aagtgttggc acatgaggag actacgatcc acaacaccgt tcagaatcag gtagcgtccc
     3181 aaaccaacca agtgggcttc gtccatcccg atattgacct tgcgagtctt cagtttagac
     3241 aacgtggcaa acatgccatc aatcaggtca taaacctttt cttcgccaat gagaagaatg
     3301 tcctctacat cacggagctc ctgcacttcc ttgacacccc atttgacaaa ggctgacagg
     3361 tgggccaagg tctggatgca tggtacaaag gtcatgtcaa actgttgagc ataggcttcg
     3421 attttctgca actcctctgc tgaataagct ccacggaaat agccaaagta aggctgccct
     3481 tcaatctggt aagtgtcttc catgtaaagc tcaaaggttg agtagcccat gagagccaag
     3541 acctcaatca tctgcttggc agaagccaca ttcagcaccg catttcgaga acagtcaacc
     3601 atgtaagcca aatcttcgta agctgcttgt tcctcaatct ctactttatc agcttctgct
     3661 agaactgtta ccaacaagga caaggcacga taaagttggt gaggtttgcg ataggtcaat
     3721 tgatagtggc caccctcacc cttgatagag atagaggctt ggtcagactg agtaacagcc
     3781 acttcaacat ctggcagaga aatgtgattt tttaatactt caatagcttg cgcttgtttg
     3841 gaactaagtc ctgtaaatct taccattggt tttcctcctg tagccagttg acaagggcac
     3901 cgtagagatt agcatctgca tgataggtgc aggcttggat aacgggtgcg accgtatatt
     3961 cttcgtaagt atcgacaaag tcttcaacag ccttcttgac accttgaata aaatctggat
     4021 tttgactgat agagcctccc agactgatga catctggatc aatcagatac tggatattga
     4081 gcaagccttg cgccaaattg cggttcatgc gctcaatggc ttcttggcaa atggcattcc
     4141 cagctgcagc ttcttggtaa atcttgcgac cgtcccaatc agtctgacca gatttttcaa
     4201 tcacgtagcg aaccatattc ccagttgacg ctagttgcga ccagttgttg agcttttcag
     4261 caggggcaag ggttgtcata tagccaaatt ctccacccaa gccgtggcga cctcggtgca
     4321 gtctgccatt gataatcata gctccaccaa tccctgtccc aatcacgaca caggctgcat
     4381 tttcaagttc tggatgagcc agtaattcac tgagtccaac gcagttggca tcattttcca
     4441 gatggacagg aatctgataa gagctaagcg cctcatacca agaaaagcca tggatgtagg
     4501 gcaccgcact gaagccatca atcacacctg tctcttgatt gaccgcacct ggaacactca
     4561 tagcaatccc actgtaatcc tgctctgaca agcgctggtc tagccaagct agtaaatcct
     4621 ccaagctttc aggcgttgga atgcttgtct tattcagtat ttttccatca ggagtcagac
     4681 tggcaaactt aattccagtc cctccgatat caatcgttgc aatggtcatt gtttttacca
     4741 taaaaagggg cgaatcagca gttagttctt actctttcgc ccctcctttc tctttatgtt
     4801 tactttttga aattagattt cttctttttt aatcaattct gtacgaattt cttgtggagc
     4861 caatagtcct gaatgaactg gatatggtcg ctcaagtaag tcaagaatgg tttgttgatg
     4921 ttctgatatg cgcacatttt cttgactcat attatagtaa cggaggacat agccttcttc
     4981 attttccgcc actttaaagg ctgttggaca aacttgcggt atgctgagaa cagaatggct
     5041 caagaggcta ccagtcgcaa ccacgcttcc ttcctgtcta gcaagctgaa ggctggtaaa
     5101 cggtgtctgc aaggctttgg cacgacgata ggctgagaag cgttcttggg cttggtggca
     5161 ttcaatcgca aattcgactt caaactcccg caagcattgt gcttctggcg ttgggaagta
     5221 gccccagtca cctagctcac ctgatgcacg caaaatggtc acggcaatgg tgttatcccc
     5281 aaggatttcg tattcattca atcccttatt ggatacagtc accccttttt catcgtcata
     5341 cagactgaca aaagcttgtt ggtgttgagg attttcagga ttttcccatg aagcagctgg
     5401 tttgtttggt cgtgtcacca cctcatagat actttcagaa tcattgcttg gacgcgtgtt
     5461 atgagtcttg accaagagac ggatacggtg atccttggca gtgttagtaa agcgagtctt
     5521 gaagcggatt tgtggattgt caacgaagac agtcaactca gtttccagag gaatgtttgt
     5581 caattcttct gaccgtccag cctcacgctt cataaactcg atgatacctt gttgctcttc
     5641 ttctagcttt tcatccgcac tgacaggcac ggtcaattca tgtttgagca agattttagc
     5701 atagcgatct gtgttttcca agacctcgtg gcccttaagc tctgcaaaga ttggctctgt
     5761 tccttttggt tggaaataga tatactcgtt tccgatgtca ccccggtctt caaagcggat
     5821 aaagtcttca taggcttcgt gagttgtctt gtcatagact gtgatgttgt catccacact
     5881 caccgttacg aatggtgtat caatcactcc gttttggtaa ataccctcac ggtgttcttg
     5941 ttttccttcc agcaattgga aggttgtcca agaaagcggt gctaggtgaa ctagaatggt
     6001 cacacgcact tgtcgagcaa tacgagcttg gcggaactta tcttttggta aatcgtattc
     6061 aaaattagct ccgaggtctt cgattgtagc ctctacagga cgaccatcca agtcctccac
     6121 acggtaactt ggcaagataa gagcagccat ctttttgtag ccttctgttg ggtgcaattc
     6181 cttgaaatca caagtcgcca catcaatcac tgtgctgaca gtatcgacct tatcatgcaa
     6241 gcctgtgtta atgacagtaa agagatagtc actttgagcc ttatccgtag caattttacc
     6301 cttccactcg ttgagcaaat tacttttaac aaagtttcct acttggttga ccttggcaaa
     6361 acgcgtttcc atctcgcggt gaacttcgtc cacgctacag ccacagatac tatcatgtgg
     6421 cgcattctgc aaaagtgttt tccaagcata ggtcaactgg tccttgtggt tgtgtccacc
     6481 agtgataata gtcaagggtt ctaccacttg ctctaggagg ttgctatttt cttggaaggc
     6541 ttgttttagg taaatacggg atgaagaagt gttggcaagt gtgtaccagc catctgtttc
     6601 ctgactggtc aactcgcctg taacagttga taagtgttca ggaagcgcac cttctacagc
     6661 ttgaacatat tcatcaaaag aactatgaac aaagattaca tccgggaaga gttcatttgc
     6721 cacacgaatg gcttcgctca gatttttctg tacaggctgg tggtcacagc cgttcatcat
     6781 caaccattgg ttggtcgaag cgtaggcacg cacatctgac aatttttgtt tccagaaggt
     6841 caaggcctca tctttgtcaa ctggaatttc attcccgtta ctgtaccagt tggcaaagag
     6901 aatacctaaa acacgactac catccacacc ctgccagtac atttctgaaa actgggatgt
     6961 aaactgctca tcttcaagga cttggttgtc aaatccaatc ggcttcacac cacgaccaaa
     7021 ggccgccacg tgaatgcctg atttttgaag aatttgaggc gcttgtccca tatttccaaa
     7081 ggtatctgga aagtagccaa tctgggttga tttaccccat ttggcagctt cttgttgacc
     7141 aatcaaggta ttgcggacat tggcttcact ggagatcaag tagtcatcct gcaagatgta
     7201 aaagggacca attttaagtt tgccctcgtc aatgtagcgt tggaccttgt cacgattttc
     7261 agggcgaatt tgtaagtagt cgtcaaggac aatagtttgt ccatccaagt ggaaactctt
     7321 gaactcaggg tcattttcaa agagatcaaa gagattgtca aacaattcca ccaactgcat
     7381 acgatggctt tcaaaaggca agtaccactc acggtcccag tgactatgtg agataatatg
     7441 tacaacaaca ttttccatga agtaaaacct cattctaaat ttaaatttta acgttttaaa
     7501 tgtaaacttg tgattctgat aagaatcggt tgagctaaag cgagcccctt agcgaatatc
     7561 caagtaatcc aagaccaact cacagaacat catgttagcc caggagaacc attcacgaga
     7621 gtagagggtc ggatcatcta catgaaagct ttcgtgcatg acacctgtac caccatcgca
     7681 ggcaaccagc tgatccagca agaatttttt ctctgcctta tctcttgttg tcaatccttg
     7741 gatagaaagg gcgattggcc agatatagcg atagaaggta tgagaacttc cgagaccgct
     7801 agcgtattct ccttggtaga aatatggatt ttcagagctc aaaatggtac gacgagtagc
     7861 ttgatacact tcatcatcga ccgaacagta gcccagatag ggcgcagcta gtagacttgg
     7921 tacatttgga tcatccatga tgctggcatt tcctaggcca tccacttcaa aagcgtaaat
     7981 cttttcgccc ttgctgttgg tggtgtaagc gtagtttttg attccttctt ggatttcatc
     8041 ctgaagacgc ttggcatcag caataacact ctggctatca gctaggttta atgctgcgaa
     8101 gatttcttgc acataaccca agactactac agcaaacata tttgacggaa tcaagtaact
     8161 atactgacag cagtcatcgc tcggacgaaa ggctgaccaa gtcatacctg tcactgcaaa
     8221 gtcaggtcca aagccatcat ttaccaaggt gtcttcctta cggtccgtat ctcggacaaa
     8281 acgataagga gagttcttgt ggtcttgttc caccgtccac agatggagaa tttccttagt
     8341 cgctgcgaca aaagtctcat caaactgact agtctcgcca gtctctttcc agaggagata
     8401 agccaactgc aaaggatagc aaagcgaatc cacctcatac ttgcgctccc agatccagcc
     8461 gttaaggtct gtgtggtcag tctcgtggtg ccctttccag ttctcctcaa tgttgaagga
     8521 gttagcatag ggatccttga gtaccaaggt catctgacgt ttgaccaaac ctgcaatggt
     8581 ctgacgcagg agggcatctc ttttagctac atgaaggtag ggtctgagtt gggctgtcga
     8641 atctcgaagc cacatggcag gaatatcccc agtcaagaca aaagttgaac catcttctaa
     8701 gatttcaacc gtattgtcca aggtatcggt gtagcaacgc tcgaaaacat ccacccactc
     8761 tggatggtcc ttagcccgct ctgctacttc atctagccat tctctaacaa tttctttcga
     8821 ataaatcatc gtgcaattcc ctctttcaaa caagtttcat gttcagtata tagcttttga
     8881 gacagaaaat acatacacaa atgatagtca tttttctaca aaatagttat ttttgaaact
     8941 ccttttctaa aggccctctt tttctgatat aatgtagaaa aacagctaaa ggaaagacta
     9001 tgaaaccact acttgaaacc atcgataccc gctttggtac tgctagcaag catgccttct
     9061 ctcggggaaa taccctgcca tacacaggcg tgccttttgg gatgaattac tttgtgcccc
     9121 agaccagtga ccagggtgga tcttggtttt ttgatccgca tctgcctatc tttcagggga
     9181 ttcgattaac tcaccagccc agtccttgga ttggggacta ctcttggctc cttctgacac
     9241 ctgtcaccag tcaactgggg ggagacagcc tctttcaccg ccagtcttct tatgatatag
     9301 ataaagcttg cttccagcct cactatttga agctcttttc tctgcgttat cagattgaaa
     9361 cccagctcac accgacttgc tatggtgctt ctattcgttt gaagcaaaag caaggcaaag
     9421 ccctctccct ctatcttcac gcagcagatg aactgacagt agagcaagta gataagcgga
     9481 ctcttgccct gcgacaagaa ggtaaaactg aaactaacaa aaattcgcta acgatgttca
     9541 ctgccctgca aatgaacacg gatattcttg ctatcagcca agaaggggga gactggcgaa
     9601 ttgacttagc aagtagtcaa accgagatgc aactagccac ttctttcatc tctccttctc
     9661 aagcactgct taatctacct caagaagatt ttgatagctg taaatcaagt gcccaagcgg
     9721 attgggaaaa tctcctccat cgttttgaca ttatagagat aggagaggct gaccgaacct
     9781 tctttgacca ctgcctctac agactcttcc tcttcccaca gactttttat gagattaatg
     9841 aatcagggca agccatccac atggatctgg ctactggtac tgtcaagcct ggtgtcctct
     9901 ttagcaacaa tggtttctgg gataccttcc gcaccacctt ccccctcttt gcccttatca
     9961 taccggagca ctatcaacgc tttttagaag gtttcctcaa tagctaccgc gatactggtt
    10021 tccttccaaa atggctggct ccagatgaac ggggtatgat gccaggtaca cttttagacg
    10081 gtattatcgc agatagcgcc tgcaaggaca tggcccctga cctagaagga gaactcttcc
    10141 aagccatgct cgaaacagcc agcaaagccg accctctcgg aatcaatggc cgccacggac
    10201 tagcccaata ccaagaacta ggttacctct ctaccgacca ccacgaaagt gttagccata
    10261 ctctagacta tgcctatagt gacttttgta tcgccaactg tgccaaaaaa ctaaaaaaca
    10321 tagaaatcgc tgaaacctac aaggctgctt cacaaaatta ccgccagcta tttgacgctg
    10381 agacaggtta catgcgagca cgagacaatc aaggaaactt tcaccctgac ttctctcctt
    10441 atagttgggg gcgagactac gctgaatgct ctgccattca agctacttta ggcgttctcc
    10501 acgacatccc tggcttaatc caactgatgg gtggaaaaga aacctttagc aactatcttt
    10561 tgaaagcctg tcaagatgct cccctctttg aaacaacagg ctatggttac gaaattcacg
    10621 aaatgagcga gatggctact gctccttttg gacaaatagc catttccaac cagcctagtt
    10681 tccacattcc ttatctcttc cgttacagcg attaccctga ctacactgcc cttcttatca
    10741 agacgctccg tcagaaagct tttcacccaa gttgggaagc ctatcctgga gatgaagaca
    10801 atggtagtct ctctgcttgg tacatctggt cagctctcgg attttatcca acctgtccag
    10861 gaaaaccaag ctatgacctc ggaatccctc tctttgacca tctacgagtc tacctcgcta
    10921 aagaagataa atggctggat atccatacta aacaaaacca caaccatttt aactttgtca
    10981 aagaatgccg actggacaaa acactcgtat caactattca acaccaagac ctcttaaaag
    11041 ctgaacaact aactttcacc ctcagctggt taccaagtca ttaataacca aaagaacctt
    11101 tgcatcgcgc aaaggttctt cttttatgaa acttggactt gaagttgatc cacaagctga
    11161 ccatccaccg ttaggttcag atagaaatcc aaggttttat ctcctgcgaa atacagggtt
    11221 ggtagatgca aatctttttt gctttcccct gcttggaatt gaaggacttg aatctcatcc
    11281 tgataggcga caccatggac acctgtcccc ggttgaatcg aaatcttagc ctctaaagag
    11341 gagctacttt cactacgttc aatcctaaaa tgaagagtct ctccctttgc cacagctagg
    11401 cttttttctg caaatgctag ttcctgaaca acttcttttt ttgataatcc aggagtttta
    11461 taaagggaaa tcttggtcag tagaggcata gattgtgctt cggtaatcat cacgcgcacc
    11521 ttctgtgcct ctactagcgg acctcgcaag aggcgcttat gaccaactgt gaagcccctg
    11581 ccaaattcct gccagacacc atccacctct acttgcacat ggaaagcaga aatacgttgg
    11641 cctaacttca aatcttctct caactcaatt acatcaaaag ttttaggaga acctaagtcg
    11701 agttctaact ggatgggcaa gtctgcatcg tttgcccaag agctggtctc aaggccgtct
    11761 gtcaaatggc gacaagcaaa gtctgcggaa agagctggac cagatacctc agctcccaga
    11821 gccaaatctt ctttatagag ctcattgcga taggtcgcaa attcataaag tcgttcaata
    11881 tcctttgcat caaagagccc agcttgattc ggcggaatat taagcaagag tggagttcct
    11941 cgccctactg agtgaaagta gatttcgacc aactcctcga gagacttagg atcctgatcc
    12001 tcatggtaga accagcctgg acggatggaa acatctgcct ctccgattga aaaaatcgtg
    12061 cccgagggat ccccgtactg aagatagttc agctctgctt ctgttcctag tttatcagga
    12121 ttcacctttt gccacagtgg atcacctgca taccctcgtt cattgccaat ccagcggata
    12181 ctggtgcctt ctgttgaaaa aatcaagcaa tcgccctgca ggtcacgaat ggtttcaaac
    12241 catttttcaa attcataatt aaccttttgc gcgccctctc ctctggcacc atccatccaa
    12301 acctcagcga acttaccagc attcccatag ttaggatttg ataagatttc cttcaactga
    12361 gccagataat aggcattgta gtccgcttct cggtccacat gatagagggg actatgggca
    12421 tcccacggtg acaggtagac ccccatatcc atatcaaact ctgtggcagc ttgggatact
    12481 tcaaggagca agtcgccctt tcctctcctc caaggactga ccttaaccga ataatctgtg
    12541 tgagctgtcg gataaaggac aaagccatcg tggtgcttga ccaccaaaat caactttttg
    12601 aagcccgttt ccttgagcac acgaacccac tcacgcgcat ccaactgact cgggttaaag
    12661 cgctcaggat cctcctgtcc agtcccccat tcttggtcat aaaaggtatt aggaccgaag
    12721 tggataaaag ctgctagttc atctcccaga taagctagct gagtctgact tggtaacggt
    12781 ccatgcggtt tgattttctt catcgttata tatctccttc tttagcaact tctcttgttc
    12841 acaaggttat ccacaggttg tggataataa aaaatgatct aaccgccagc tctttccaac
    12901 tccaactggt aggctcagga ttttgctcct caatgttccc tcttcaaaca gctctgtcat
    12961 atggttctgc taacacctct cagaatgcta ctctccataa ttattctcag gcttctttca
    13021 ggtcattgtg tgaagaccat tcactctcca gacttttcca cagattgtga ataagtgatt
    13081 attctaaact atcatctcgt cgactataac aaaaaataaa aagagggtca ataagataat
    13141 tatctcttcc taaatactca gaaaagattc tataatttgt acaattgagc aagaaatgct
    13201 tctccaaaat ctatcttcaa aagaatgttg catcttttta tcctttcaca caaacatttc
    13261 tctttataca gatccaagtg ataaccagat gataattgga cactttttgg ttatttgtgt
    13321 attttttgaa aaatttttta aaaaaatttt atatcctctt taatagaa
//
LOCUS       DAVKGC010000047        13275 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKGC010000047 DAVKGC010000000
VERSION     DAVKGC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13275
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.047"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            41..871
                     /locus_tag="VW049_001170"
     CDS             41..871
                     /locus_tag="VW049_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9432304.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NSSPKPFNYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAV
                     NNISYTKERAEKYLYAAPTVKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQL
                     EDYNKQHSDNPTILNYTKANLQQIMGHLSDGQFDYKIFDKITVETVIKNQGLDNLKVI
                     DLPSDQQPYVYPLLAKGQDELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            912..989
                     /locus_tag="VW049_001171"
                     /pseudo
     CDS             912..989
                     /locus_tag="VW049_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            1093..1923
                     /locus_tag="VW049_001172"
     CDS             1093..1923
                     /locus_tag="VW049_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9432305.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2077..2931
                     /locus_tag="VW049_001173"
     CDS             2077..2931
                     /locus_tag="VW049_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9432306.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3031..4404
                     /locus_tag="VW049_001174"
     CDS             3031..4404
                     /locus_tag="VW049_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9432307.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4397..5458
                     /locus_tag="VW049_001175"
     CDS             4397..5458
                     /locus_tag="VW049_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9432308.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5460..6152
                     /locus_tag="VW049_001176"
     CDS             5460..6152
                     /locus_tag="VW049_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9432309.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6182..6751)
                     /locus_tag="VW049_001177"
     CDS             complement(6182..6751)
                     /locus_tag="VW049_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9432310.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIAFFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            6886..7500
                     /locus_tag="VW049_001178"
     CDS             6886..7500
                     /locus_tag="VW049_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9432311.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7502..9148
                     /locus_tag="VW049_001179"
     CDS             7502..9148
                     /locus_tag="VW049_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9432312.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9160..10446
                     /locus_tag="VW049_001180"
     CDS             9160..10446
                     /locus_tag="VW049_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9432313.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10498..11031
                     /locus_tag="VW049_001181"
     CDS             10498..11031
                     /locus_tag="VW049_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9432314.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDETMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            11109..11579
                     /gene="nrdI"
                     /locus_tag="VW049_001182"
     CDS             11109..11579
                     /gene="nrdI"
                     /locus_tag="VW049_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9432315.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(11872..13134)
                     /locus_tag="VW049_001183"
     CDS             complement(11872..13134)
                     /locus_tag="VW049_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9432316.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         4019 a         2474 c         3009 g         3773 t
ORIGIN      
        1 ctgttaagat tataaggtat tctattttgg aggaaatgac atgaaaaaaa tcgttaaata
       61 ctcatctctt gctgccctag ggcttgttgc tgcaggtgtg ctagcagctt gctcaggtgg
      121 tgctaagaaa gaaggacaag cagctagcaa gaaagaaatt attgttgcaa ctaattcttc
      181 accaaaacca ttcaactatg aagaaaatgg cgaattgact ggttacgaga ttgaagtcgt
      241 tcgcgctatc tttaaagact ctgacaagta tgatgtcaag tttgagaaga cagagtggtc
      301 aggtgtcttt gcgggtcttg acgctgaccg ttaccaaatg gctgttaaca atatcagcta
      361 cactaaagaa cgtgcggaga aataccttta tgcagcccca actgttaaag accctaacgt
      421 tcttgtagtc aagaaagacg attctagcat caaatcgctt gatgatgttg gtggaaaatc
      481 aacagaagtt gttcaaggaa caacatctgc taaacagtta gaagactaca acaaacaaca
      541 ctcagataat ccaactatcc ttaactatac taaagcaaac ttacaacaaa tcatgggcca
      601 cttgagcgat ggtcagtttg actacaagat ttttgataaa atcactgttg aaacagtcat
      661 caagaaccaa ggtttggaca acttgaaagt catcgatctt ccaagcgacc aacaaccgta
      721 cgtttaccca cttcttgcta aaggtcaaga tgagttgaaa acatttgtag acaaacgtat
      781 ccaagaactc tacaaagacg gaactcttga aaaattgtct aaacaattct tcggagacac
      841 ttacctaccg gcagaagctg atattaaata atttcttgaa atcatccatt ctgagtaaga
      901 tgggtgattt ttcagttcgc tactgcaatc tagtcggaac agttctcttt ggagtggtct
      961 tgattgccat cttgcgtttg gtcttttaaa ttacccataa aaaaaactta tcacagagat
     1021 agatatcata taatggcgta aatgctcttt ttctgttaag attataaggt attctatttt
     1081 ggaggaaatg acatgaaaaa aatcgttaaa tactcatctc ttgctgccct aggacttgtt
     1141 gctgcaggtg tgcttgcggc ttgctcaggt ggtgctaaga aagaaggaga agcagctagc
     1201 aagaaagaaa tcatcgttgc aaccaatgga tcaccaaggc catttatcta tgaagaaaat
     1261 ggcgaattga ctggttacga gattgaagtc gttcgcgcta tctttaaaga ttctgacaaa
     1321 tatgatgtca agtttgaaaa gacagaatgg tcaggtgtct ttgctggtct tgacgctgac
     1381 cgttacaata tggctgtcaa caatcttagc tacactaaag aacgtgcgga gaaatacctc
     1441 tatgccgcac caattgccca aaatcctaat gtccttgtcg tgaagaaaga tgactctagt
     1501 atcaagtctc tcgatgatat cggtggaaaa tcgacggaag tcgttcaagc cactacatca
     1561 gctaagcagt tagaagcata caatgctgaa cacacggaca acccaactat ccttaactat
     1621 actaaggcag acttccaaca aatcatggta cgtttgagcg atggacaatt tgactacaag
     1681 atttttgata aaatcggtgt tgaaacagtg atcaagaacc aaggtttgga caacttgaaa
     1741 gttatcgaac ttccaagcga ccaacaaccg tacgtttacc cacttcttgc tcaaggtcaa
     1801 gatgagttga aatcatttgt agacaaacgc atcaaagaac tttataaaga tggaactctt
     1861 gaaaaattgt ctaaacaatt cttcggagac acttatctac cggcagaagc tgatattaaa
     1921 taatttcttg aaatcatcca ttctgagtaa ggtgggtggt ttctcagttt ttagggatat
     1981 agttttaaac tatatatctg actatctaat aacaatttgt gaaatcaaac aaattgtgag
     2041 atactagtac ggtattattt ttaaggagaa agaatcatga aaatcaaaaa atggcttggc
     2101 ctagcagccc ttgctacagt cgcaggtttg gctcttgcag cttgcggaaa ctcagaaaag
     2161 aaagcagaca atgcaacgac tatcaaaatc gcaactgtta accgtagcgg ttctgaagaa
     2221 aaacgttggg acaaaatcca agaattggtt aaaaaagacg gaattacctt ggaatttaca
     2281 gagttcacag actactcaca accaaacaaa gcaactgctg atggcgaagt agatttgaac
     2341 gctttccaac actataactt cttgaacaac tggaacaaag aaaacggaaa agaccttgta
     2401 gcgattgcag atacttacat ctctccaatc cgcctttact caggtttgaa tggaagtgcc
     2461 aacaagtaca ctaaagtaga agacatccca gcaaacggag aaatcgctgt accaaatgac
     2521 gctacaaacg aaagccgtgc gctttacttg cttcaatcag ctggcttgat taaattggat
     2581 gtttctggaa ctgctcttgc aacagttgcc aacatcaaag aaaatccaaa gaacttgaaa
     2641 atcactgaat tggacgctag ccaaacagct cgttcattgt catcagttga cgctgccgtt
     2701 gtaaacaata ccttcgttac agaagcaaaa ttggactaca agaaagcact tttcaaagaa
     2761 caagctgatg aaaactcaaa acaatggtac aacatcattg ttgcgaaaaa agattgggaa
     2821 acatcaccta aggctgatgc tatcaagaaa gtaatcgcag cttaccacac agatgacgtg
     2881 aaaaaagtta tcgaagaatc atcagacggt ttggatcaac cagtttggta ataagaaaca
     2941 gggaggtggg agagagaatt ccacctcttg cttttgtata gagcacggag tgtaaagaag
     3001 actattcgtt catagaaagg tagagagaat atggtttttc ctagcgaaca agaacagatt
     3061 gaaaaatttg aaaaggatca tgtagcccag cattattttg aggttttgcg taccttgatt
     3121 tctaagaaat cagtctttgc ccagcaggtt ggactcaagg aagtcgcaaa ttatctgggt
     3181 gagattttca agcgtgttgg agctgaagtg gagattgatg agagctatac agctcccttt
     3241 gtcatggcac atttcaagag ttcgcgtcca gatgccaaga ccttgatttt ctataaccac
     3301 tatgacactg tgccagcgga tggggatcag gtctggacag aggatccttt tacgctttcg
     3361 gtccgcaatg gcttcatgta tgggcgtggg gttgatgacg acaagggtca tatcacagct
     3421 cgcttgagtg ctttgagaaa atatatgcag caccatgatg atttacctgt caatatcagc
     3481 tttatcatgg agggagcgga ggaatcggct tcaacagacc tagataagta tttggaaaag
     3541 catgcagaca aactccgtgg ggcggatttg ttggtctggg aacaagggac caaaaatgcc
     3601 ttggaacagc tggaaatttc tggtggcaat aaggggattg tgacctttga tgccaaggta
     3661 aaaagcgctg atgtggatat ccactcgagt tatggtggtg ttgtggaatc agctccttgg
     3721 tatctcctcc aagccttaca gtctcttcgt gctgcggatg gccgtatctt ggttgaaggc
     3781 ttgtacgaag aagtacaaga gcccaatgaa cgagaaatgg ccttgctaga aacttatggt
     3841 caacgaaacc cagaggaagt tagtcggatt tatggattgg agttgcctct cttacaggag
     3901 gagcggatgg cctttctaaa acgtttcttt ttcgagccag cgcttaatat cgaaggaatc
     3961 cagtctggtt atcaaggtca gggtgttaag actattttgc ctgcagaagc cagtgccaag
     4021 ctagaggttc gtctggttcc gggtctagaa ccgcatgatg ttctggaaaa aattcggaaa
     4081 cagctagaca aaaatggctt tgataaggta gaattatact ataccttggg agagatgagc
     4141 tatcgaagcg atatgagcgc accagccatt ctcaatgtga tcgagttggc caagaaattc
     4201 tatccacagg gcgtttcagt cttgccgacg acagcgggga caggacctat gcatacggtc
     4261 tttgatgccc tagaggtacc aatggttgca ttcggtctag gaaatgccaa tagccgagac
     4321 cacggtggag atgaaaatgt gcgaatcgct gattattaca cccatatcga attagtagag
     4381 gagctgatta gaagctatga gtagagatat tatcaagtta gatcagatcg atgtgacttt
     4441 tcaccaaaag aagagaacca tcacagcggt taaggatgtg accattcaca tccaagaagg
     4501 ggatatctac ggaatcgttg gatattctgg agcagggaaa tcaacccttg tacgggtgat
     4561 taatctcttg caaaaaccat ctgcagggaa aattaccatt gacgacgatg tgatttttga
     4621 cggcaaggtg accttgacgg cagagcagtt gcgtcgtaaa cgtcaagata tcgggatgat
     4681 tttccagcat tttaacctga tgagccaaaa gacagcagag gagaatgtag cctttgccct
     4741 taaacactct ggactcagca aggaagaaaa gaaggctaaa gtagctaagt tgttggactt
     4801 ggttggtttg gcagatcgtg ctgaaaacta cccttcacaa ctatctggag ggcaaaaaca
     4861 gcgtgtggca attgcgcgtg ccttggccaa tgatccaaaa atcttgattt cagacgagtc
     4921 aacttctgcc cttgacccta agacaaccaa gcagattttg gccttgttgc aagatttgaa
     4981 ccaaaaatta ggattgacag ttgtcttgat tacgcatgaa atgcagattg tcaaagacat
     5041 tgccaaccgt gtggcggtta tgcaggatgg gcatttgatt gaagagggaa gtgtccttga
     5101 aatcttctca aaccctaaac aacctttgac tcaagacttt atctcaacag ccacaggtat
     5161 tgacgaagcc atggtcaaaa tcgagaagca agaaatcgtg gaacacttgt ctgaaaacag
     5221 tctcttggtg caactcaagt acgctggagc ttcaacagac gagccacttt tgaatgaatt
     5281 gtacaagcat taccaagtaa tggctaatat tctctatggg aatatcgaaa ttctcgatgg
     5341 tactcctgtt ggagaattgg tggtggtctt gtcaggtgaa aaagcagcgt tggcaggtgc
     5401 ccaagaagcc attcgtcaag caggtgtaca actaaaagta ttgaagggag tacagtaaga
     5461 tggaatcatt gattcaaacc tatttaccaa atgtctataa aatgggttgg gctggtcagg
     5521 caggctgggg aacggctatc tacttaactc tttatatgac agttctttcc ttcattatcg
     5581 gaggcttctt ggggctagtg gcaggtctct ttctcgtctt gacagcgcca ggtggtgtct
     5641 tggagaataa agtcgtattc tggattttag acaaaattac ctcaattttt cgtgcggttc
     5701 cctttatcat cctcttggca atcttgtcac cactttctca cttgattgtt aaaacaagta
     5761 tcgggccaaa tgcagccctt gtcccacttt cttttgcagt ctttgccttc tttgcccgtc
     5821 aggtgcaggt tgtcttggct gaattggatg gcggtgtcat tgaggcggct caagcgagcg
     5881 gagcgacttt ctgggacatc gtgggtgttt acctatcaga aggtcttcca gatttgatcc
     5941 gtgtgacgac tgtgaccttg atttcccttg ttggggaaac agctatggcc ggtgcggttg
     6001 gagctggtgg tatcggtaac gtagccatcg cttatggatt taaccgctac aatcacgatg
     6061 tgaccatctt ggcaaccatt attatcattt tgattatctt tgcaatccaa ttcttgggag
     6121 atttcttgac taagaaattg agccataaat aaaaaagagc cgcgtggctc tttttaactg
     6181 atcagatttt ctgtgcaaat tttttactca aggcttgtcc aatcaaggca cccactaggg
     6241 ctccgatgac aatacttgcg ataaatagaa ggacagttcc agggtttgga gcgaccatga
     6301 tacggtcgat atattcttgg gattttcctc ttgccagaag agtagccata taggctttgg
     6361 gcgcaatcca cataagcaag attggtcctg ttgtactaaa ggcgaaaata atgaaagaaa
     6421 ggaagttctt tgttttgtcc ttgtattttc ctaaatgagc tacttcatct gctaggaggc
     6481 cacagataat tccaggaagg aaggaaccag caccgtgttt agttcccaag aaaaagaagg
     6541 caatgacaag gccgatagtg gtaatggctc caaagcgcgg aacttttgcg actaggatca
     6601 tatagacgct accgccgaca agggcagtaa aggcaggcgc ataaaacatg tttccagttt
     6661 ggtcaaagag attgcccaaa aggacaccaa tccccatgca gaggaagtaa agaactgcaa
     6721 aaagcagtgt agttaagata gatttcttca tgaattgtct cctgataatt ttttcacaat
     6781 tctcatttta ccatggtttg gtgggattgt aaatggatac tagaagtttt tgaaaacgct
     6841 tgaaatatga tataatggtt tcatagttat ttttagggga atatcatggg tagattttta
     6901 gactttgtct ttaatcgttt ctttttaggg atgattgcga cagccttctt ttggctatta
     6961 actttagcag gagggattat ccttggtcta gcgccggcta gtgccacctt gatgagctta
     7021 tatgcagaac atggttatag ctttcgggaa tacagtttga aggaggcttg gtctctttac
     7081 aagcaaaatt ttgtctcaag caacctgatt ttctatagct ttttaggtgt gggtctagtt
     7141 ttgacctatg gtttgtatct cttggtgcaa ttgcctcatc agaccattgt tcatttgatt
     7201 gcgacccttt tgaatgtcct agtagttgcc ctgatctttt tggcttatac agtatcttta
     7261 aaattacaag tttattttgc cttgtcctat cgaaatagtc tcaaattatc cttgattggc
     7321 atctttatga gtctagcagc tgtggctaag gttctccttg ggactgtgct acttgtagca
     7381 attggttatt atatgcctgc cctgctattt tttgtaggaa ttgggatgtg gcatttcttt
     7441 atcagtgata tgttggaacc tgtctatgaa atcatccatg aaaaattggc gacaaaatag
     7501 aatgaagcag ttttggctac atacgcttct aagaacctat agttcagtga tgatcattat
     7561 cattgcgagt tttgcaatct tactctctta cgctgactgg gattcacgtg aaaaggaagc
     7621 ccagagagta gcccagcgtg taactgctag aacagtgagt gaaattgaat attaccatag
     7681 agagtcaacc cagatagctc aggctttagt tgaaaatcaa gctcgtatcg agggaatcta
     7741 taaatacttt agccttagca tgccagacta tttttactgg caattagagc ggaaagcttc
     7801 gccttatata tcagtctctc tgtatgaaaa tgttgatgac ctctatgttc gaaatgattt
     7861 tgtaactggg gtggccattg cttttcaaga ttacaaggaa gtctatgttt ctactaaaga
     7921 caaacgtagt ggagaaaaaa tcagggctga ggatttcaaa ccagcaggaa atagttttgc
     7981 cattccggtg tcagatccag tgtcagatca agatttagga gtgatttaca tctccttgga
     8041 tcctgctgtt ttataccatg ccattgataa tactagaggt catactccga tggcagtaac
     8101 agtgacctca ccttttgata cggagatttt tcatattggt gagacagttg atagggagag
     8161 tgaaaattgg ctagttggct taacttctca tggatatcag gttcaggtgg cagttcctaa
     8221 aaactttgtt ttacaaggaa cagtgactag ctctgctttg attgtgggtt tgagccttct
     8281 ctttattgtc attctttatc tgactttgag gcagactttt gctaattacc aaaagcaggt
     8341 agtggattta gtggaatcca ttcaagtcat tgctcaaggc gaagaggggc gtcggattga
     8401 catttccgaa aaagatcagg aattactcct aatcgcggag acgaccaatg atatgttgga
     8461 tcgattggaa aagaatatcc atgatattta ccagttagag cttagtcaaa aagatgccaa
     8521 tatgcgagcc ttgcaggcgc aaatcaatcc tcattttatg tataatacgc tggagttctt
     8581 gcgcatgtat gcagttatgc agagtcaaga tgagttggca gatatcattt atgaattcag
     8641 tagtctcttg cgtaacaata tttccgacga aagagagacc ctcctcaaac aggaattaga
     8701 attttgccgt aaatacagct atctctgcat ggttcgctat cccaagtcca ttgcctatgg
     8761 tttcaagata gatccagagt tagagaatat gaagattccc aagtttacct tgcaaccgct
     8821 ggtagaaaac tatttcgcgc atggtgttga ccacaggagg acagataatg tgattagcat
     8881 caaggctctt aaacaggatg ggtttgtgga aattttggtg gtcgataatg gtagaggaat
     8941 gtcggctgaa aagttggcaa atatccgaga aaaattaagt cagagatatt ttgaacacca
     9001 agccagctac agtgatcaaa ggcagtctat cgggattgtc aatgtacacg agcgttttgt
     9061 gctctatttt ggagaccgct atgccattac gatagagtct gcagagcaag ccggtgttca
     9121 gtatcgtatt acaattcaag atgagtagaa agggagaaaa tgtataaagt attattagta
     9181 gatgatgagt acatggtgac agaaggtctg aagcgtttga ttccctttga taagtgggat
     9241 atggaggtcg tcgcaacagc cagtcatgcc gatgaagctc tagaatatgt tcaggaaaat
     9301 cctgtcgatg tcatcatttc cgatgtcaat atgccagaca aaacagggct tgatatgatt
     9361 cgggagatga aagagatctt accagatgct gcctatatcc tgctctcagg ttatcaggag
     9421 tttgattatg taaaaagagc aatgaacctt agtgtggtgg actatttggt caagcctgtt
     9481 gataaggtag agctgggaaa tctgctggag aagattgcag gtcagctcgg cgagagaggg
     9541 aagaaaagtc agactcttag tcaagaatta gacgaggctg gatttgttag ttatttaggg
     9601 gataaggaga attggtggat aggtctatcc aaggaaaaac aaggttcctt caccattccc
     9661 tactatgtct tgggtcaaga ctggcagatt ttcatttctg gccaacccct agatggttta
     9721 gtcgttacac cttttgaagc tccttatcaa gaacactttg aacgctggaa gctgaatgct
     9781 gagaaaaccc tcttttacgg ttctgtaaat ttgcagcagt ctgagagtct ctttgcctat
     9841 tacgaaccga tttatagggt tatcattcag ggaaatctca atcaaatcgt agaagagtta
     9901 aatctcttgg agaaggtagt tcttgaaaat acgccgcgtg ttccgattac taaacagctt
     9961 tttattcagt ttgtcatgga tgttttccat ttatttgaac atctcaaagc tgatgacctg
    10021 acagacatcg tcaaaaccat tcatgctatt caatccttcg atgaattggt tccttatatc
    10081 aaggaaactc tgaccagctt tttcggtcaa taccgtatga atgaaaatgt ggtcagtgtg
    10141 ctggaagtca ttggtcgtga ttaccaaaaa gagctttccc tcaaggatat cagtaaggct
    10201 ctctttatca atcctgtcta tctagggcag ttgattaagc gtgaaaccga ttcgactttt
    10261 gcagagttac taaacaaaca acgtattaag gctgcccaac aacttttgct ttcaactagt
    10321 gacagcatcg aagatatttg ttatgctgtt ggttacagta accttggata tttctataaa
    10381 gttttccgaa aattgtgcgg aaaatcgcca aaagcctacc gaaaacaggt agaaactata
    10441 ctataagatt tgtattcctt tacaaaaggt gctataatat tatcaggagg tcctatcatg
    10501 aaaaagaaac caatttatct atgggtcttg ctaatcttgt ctgcccttat ttcagttccg
    10561 tctctgtttg gaattgtgag tcccttgcct agtaaagaag cccttcgtgc tgctcagaaa
    10621 caagttgcag gggtcaatgc tcagcaatta gaagaccagc ttaattatac ctatagagta
    10681 gcagaagcat ctcattctat ttttaatgtt gctttgattg tgctatctac tattttagtg
    10741 gtcgtagcga ttgtcttcct tgttcgtaaa aatttgcaat atgcaaacta tacttacgtt
    10801 ggctatgttt tgttagctat tattggttcg atttatggct atgttggttt acaagacgct
    10861 gtgcagttgg ttcaagatga gaccatgcgt ttgacagtga gtattggttc aaaagctgtt
    10921 agcattttct atatcgtcat caatgttctc ttcctagccc ttgtcttcta taagatgtgg
    10981 cgacaacaga aagccttggc agaagaagag gaaacagaag agcttgccta agtattgaca
    11041 aaaaagaact atcaagatac aatcttggta gttgtttttt gattatagat aaaatcatgg
    11101 aggtacatat gaagacaatt tctttagttt atattagttt gagtggcaac actgaaagtt
    11161 ttgtgacgcg cttgaaagac tatctcttgt cccagtacaa agggattgag gttcaaaaga
    11221 ttcatatcaa ggatttagtc aaggaaggca aaaatttcta tgaaatggac catccctatg
    11281 tcgctttttt gccgacctat ctcgaaggtg ggaatggcgt ggataacgga gatgttgaga
    11341 ttttgacgac accggtggga gattttattg cctatggtaa caatgctagt aagtgttttg
    11401 gtgtggttgg gtcaggaaat cgtaacttta ataaccaata ctgcctaaca gccaagcaat
    11461 acagtcaacg ctttggcttc cctgtattgg ctgactttga aatgcgaggc atgctggaag
    11521 atatcaaaca tgtcgcagct attatcgcag atttgtatga gttggaaaag gagaattaat
    11581 actcaatgaa aatcaaagag caaactagga agctagtcgc aggttgctca aagcacagct
    11641 ttgaggttgc agatagagct gacgtggttt gaagagattc tcgaatagta taaagttctt
    11701 ataagaggtg gttgaagttg ttcaactgtt ttttgagtat aaacagtctt taagaacgta
    11761 aaaaagcata agttcaggtc ttcaaaacct tgttcttatg ctttttatca tagaaatatt
    11821 tgctgaattt tctcttgaaa tgaaacctta tcttcatctc gcgcttgtcc cttaccctgc
    11881 ttttccgatt gcgagtgcgt agataaagaa gatggcgaag ccaaagagga aaatcaatcc
    11941 cgcaatggca aggtgtttga gccaagaagg aagatttttg ttttcacgcg ttaccaaggc
    12001 gtaattcaaa agagcaaaga aaggtgttgt caggaaagag ccaatcatgg caaagcggag
    12061 catggttgaa acctgaccag cgaagaactt gataatgacg ataccgatga tagcagtgat
    12121 ggtcatccag atgttcaaag atttacgatt gtcctctttt tgactgatta gcagtcggag
    12181 agattcctga ttaacgcgag aatagccatc gataacagtt ataactgttc caaagataca
    12241 gaggaaggca ataaaggtaa tcaagtaacg ggaccattcg ccaagaacag aggcatacat
    12301 gcccacgaat tgagagatgt atttggctga agcagcttca accgcctgcc ctgtaggata
    12361 ctgaatcagt gctcccagtg ccacaaagaa gacggctagg atagctgttc caatataacc
    12421 agtgttaaag tcaaacagag cgtcttctgt gttaaagttg acggtctttc tcttttcagc
    12481 tgaccaaagt gaattgatgg ctgaaatttc aataggagcc ggcatccatc ctaggaggga
    12541 gacgatgaag ggcagagctg ccatttgcca aggtgtcttc tcgacaaaat cagaactgta
    12601 ttctggatgc ttgaccgccg caatgataac tgcaagaaca gtcgcaatgg ttaaggcaga
    12661 cataatccat ttgaccatgc cgtctaaaag tttgtagcct ccaaagagta gcatagccca
    12721 aatgattgca acgagaatga gggaccactg agtaatgcta agtccaatca ttgggaaggc
    12781 actggcgatg atagctgagc acagaatggc aacaccagcc gtgttgacca tagccgaaaa
    12841 gacattgagg ataaagaaaa tccagagata gagttttcct ttttcggcat aaccttcaac
    12901 caaagtcttt ccagtatcag ctgtgtattc agcaccaaaa cggaaaaatg gatatttaaa
    12961 gacattggct aagatgacca agagaagtag agaccaaccg taagaaccgc cagcttgagt
    13021 tgaggataca atgtgggaac ctccaacagc ggcagttgcc attaggattc caggtcccat
    13081 tgcttttagc ttgcttgccc aggttgattg atttaaggaa atagcttgcg acatgataaa
    13141 tactcctttt tgaattttca gaataatctg tatatgtaat ctatattcta caaaaaattt
    13201 tggaaaaatg caagagtatt ttgaaaaaag atagaaaatt tacaaagaaa atctgaaata
    13261 actgttatga tatag
//
LOCUS       DAVKGC010000048        13184 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKGC010000048 DAVKGC010000000
VERSION     DAVKGC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13184
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.048"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(1051..2277)
                     /locus_tag="VW049_001184"
     CDS             complement(1051..2277)
                     /locus_tag="VW049_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9432317.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(2320..3012)
                     /locus_tag="VW049_001185"
     CDS             complement(2320..3012)
                     /locus_tag="VW049_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9432318.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLAFVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(3185..4936)
                     /locus_tag="VW049_001186"
     CDS             complement(3185..4936)
                     /locus_tag="VW049_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432319.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLRG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(4926..6671)
                     /locus_tag="VW049_001187"
     CDS             complement(4926..6671)
                     /locus_tag="VW049_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432320.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQTAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6877..7173)
                     /locus_tag="VW049_001188"
     CDS             complement(6877..7173)
                     /locus_tag="VW049_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9432321.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(7326..7781)
                     /locus_tag="VW049_001189"
                     /pseudo
     CDS             complement(7326..7781)
                     /locus_tag="VW049_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7915..8742
                     /gene="xth"
                     /locus_tag="VW049_001190"
     CDS             7915..8742
                     /gene="xth"
                     /locus_tag="VW049_001190"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9432322.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            8754..8852
                     /locus_tag="VW049_001191"
     CDS             8754..8852
                     /locus_tag="VW049_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9432323.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEY"
     gene            8897..9136
                     /locus_tag="VW049_001192"
     CDS             8897..9136
                     /locus_tag="VW049_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9432324.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      9522..9617
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10017..10598
                     /locus_tag="VW049_001193"
     CDS             10017..10598
                     /locus_tag="VW049_001193"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9432325.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            10598..11860
                     /locus_tag="VW049_001194"
     CDS             10598..11860
                     /locus_tag="VW049_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9432326.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11911..12372)
                     /locus_tag="VW049_001195"
     CDS             complement(11911..12372)
                     /locus_tag="VW049_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEU9432327.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(12369..13133)
                     /locus_tag="VW049_001196"
     CDS             complement(12369..13133)
                     /locus_tag="VW049_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEU9432328.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
BASE COUNT         3886 a         2856 c         2586 g         3856 t
ORIGIN      
        1 aaggaataac acaagaaaat attaaaattt atactcttcg gaaatctctt caaactaggt
       61 cagtgccgcc ttgacctaag tctaggttac tgacttggtc agttctatag tagactgaaa
      121 ctataatagt acacctctac ttctaaaaca ttcttagaaa tcgatttgac tgtcccgatc
      181 gatttgtcct attcttattt cattttatta tatctacaac ctcaaagcag ggttttgagc
      241 tatctatggc caacttccta gtttgctctt tagccttcca ttcaaaagtg atgctatctt
      301 tcttagcttg aatataaaat atcccattta ctatatttaa atttgttgat atgttgtaaa
      361 ttataaatga aagaacaaat atcttgtcat acaagcattc gccagggata tttacatgaa
      421 tatcgctcat atggcggatt gaaactaaaa cactacaata tatttcttaa atagaccttc
      481 tataaaagct agatttaacc tcgataaaaa tagatgcagt gcttctaaaa tactggaata
      541 gggcatatag gtaatcgtag acaagtgttt tatagattgt ctcgcaggag atctaatgat
      601 gtatagcaag catcactaaa catatgggat atttgcgttg ttataatatc aacatgctat
      661 aaattctatc atgcaaaaga atgactagaa catgaaagac aggcacaaaa ctttgaacaa
      721 tattctgaaa acaaaaaaaa gacgctagtt tccacaatct cactcatgga aactcacgtc
      781 tttcaaaaaa tagggtgaat tgaaaactag aacaagacac tagattttct aaatgatgtc
      841 tagtaaggat tctaaatgct ataccaaatt gctcacatct tatttcaaac cacgggactc
      901 ccacaagaaa ggaaaatcct ataaaaattc tctctctgag gagaaagagg cctcaactac
      961 aggtgtaaac tattatcaat tgaaaacaac agtttttaca ctcaattaga ctttttaggg
     1021 acaggatcac tttcgcttga ctgtagtttg tcattttttt gataaagtta gcactttttc
     1081 agaaactgtt ttgaaagtct caatgatata gtctacttct tcatcgctta atttagtatg
     1141 aagagggagg gtaatttcat tctcaaagaa ggcataggcc ttaggatagt tcgtcatatc
     1201 aaatccaaga ttcttatagg ctgtcaagag aggaagcggt ttgtagtgaa cattacttgc
     1261 aattcctgct ttagccaatt cttggatgat gaggttgcgt tcttctaggc ttgctccttc
     1321 tacacgggtg atgtagaggt ggcgtgaaga ttcgacagtt tcagtcttgt gtgccaaagg
     1381 atggatgcga gaacctgcaa aaccactatc atagcggtcc acaatgtcct tacggcgttg
     1441 caacaaactt ggatagcggt ccaattgtac caaaccaagt gaagccatga tatcggtcat
     1501 gttgcactta taggctggtg taacgatatc gtattcccat gaccccaatt gcatcttggc
     1561 aagagcatcc ttagtttgcc cgtgaaggga aaggatttgg aattccttgt acatctcttc
     1621 gtcatcaatc actggattgg ctttccaagt cgcacttcca ccttctgccg ttgtaaagtt
     1681 cttaacggca tggaatgaga aggaagtaaa gtcagcgata gaaccagaag gttgtccttt
     1741 ataagtagat cccaaagcgt gggcactatc agagacaatg acaatacggt taaaggcctt
     1801 ttgccacttg cttgaagcgg taaagaagtc acgttttttc tccacgactt ggaacaaacg
     1861 gtcataatcg caaacaatcc ctgcgagctc tactgggata atcaccttag ttttctcagt
     1921 gatagcttgc tcaagcaggt catagtccat ctcaaacgta tctgcttgga tatccaccat
     1981 gacaggggtt gctcccacgt gcgtaatgac actacatgaa gccgtatagg tcatggctgg
     2041 aacgatgact tcatcaccag gtcccacttc caaaacgcgt aaaatcaact ccagagcggc
     2101 tgtcgcagag ttgagacaaa cagtcttagg tgtctgtgtg taaagagaca agcggcgctc
     2161 cagttctttt gttttaggac ctgttgtgat ccaaccagaa cgcagggtat ccgctacttc
     2221 agcaatttct gcttctgtga tatcaggcgg tgaaaatgga atattgtaat ttggcattga
     2281 tttgctcctt ttatctgtac tcattttctt atgactactt tattttagta cctcaaacac
     2341 ggtttgaaac atgattttga tgtccccaaa gaaactaaac tctcggagat aggcgaggtt
     2401 atagcgcatc ttttcaggaa gaacatgctc cacataggcc tgatcaactg acagaccttt
     2461 ctccgtcatt tgactgatga tggtgtcctc atccttgtag ttgatgctgg ctggagaggt
     2521 aattcctgct tgcaagagca aggttgccat catttcaggg ctatactgct ctgtataacg
     2581 tggcacttca ggtcgtgtac cgacaaagga catctcacct ttaaggacat tgaccaactg
     2641 aggcagttcg tccaaacgga cacgtcggat gaaatttcca accttggtaa tacggctatc
     2701 gttagcagaa gtcaccagac ttcctttttt atccgcatcc gtcaccatgg tacggaactt
     2761 ccaaatcttg aaccgacggt tgtactgggt cacacgctct tgcttgtaaa tcactggccc
     2821 cttgctatcc aacttgatcc aaatgctcaa gatgagaaag atgggagagg tcagaaccag
     2881 taagacaaag gctagaaccc agtccaagca acgcttgaaa atcagcgaac ccttcctttt
     2941 agagacaagc tggtaataag actcaacctc gcttgatttc atttccacag gcaagtcttc
     3001 ccatttcagc atgctgttct cctttgtttt tattatacta tttgtcaaca tttttcatta
     3061 taccacaaaa tggaaaaggc ggtgaaagaa atctgtaatt tgaaggattt ccttctagta
     3121 ctcaatgaaa atcaaagtac agctttgaga ttgcagataa agctgacatg gtttaaagag
     3181 attttcaaag agtataggcc atggcccctg cctgcaaact atacatcttg tgataggttc
     3241 ctctcagagt caagagttcc tcatgggttc cactctcgat aatgcgtccc ttatccaaga
     3301 catagatgca gttggcgtct tgaatagtag aaaggcggtg agcgatagca atagttgttc
     3361 ggccctgtct catcttcgcc agagaagctt gaaccaaact ttctgtttca gagtcaatat
     3421 tggctgtcgc ttcatccaaa atcaggattt taggctggct ggcgactgtt ctagcaaagg
     3481 caagaagctg gcgctgccca gtagagaagc tcgaaccacg ctcggaaaca ggggagtcgt
     3541 acccctgagg aagttcttga ataaaggaat ctgcatccac aaaggctgcc gcagcctgaa
     3601 cctgctcatc actgatttct tggtacatgg cgatattgga cttaatagtt ccatgataga
     3661 ggaagggttc ctgtaaaacc aaaccgatgt tttttctcag ctcttcttga ctgaaatccc
     3721 tgatatccac atcatccaag agaactctcc ctgactggaa ttcataaaag cgcatgagga
     3781 cattgataat agacgatttc cctgaacctg tatgacctac aaaggcaatg gtttcaccct
     3841 tattaacaga gaaagaaatg tcatccagaa tcggatgttt accgtcatac gagaaacaca
     3901 catgttcaaa acggatattg ccttctttga ctttggcttg cccattttct tgaaggggct
     3961 cataggtcct ctcatcgatt aaggcaaaga cacggcctgc agaaaccata gacgtttgca
     4021 gtgttgaaaa gttttgcgtc acctcaatca aagggtcaaa gaggcggttg atgtactgga
     4081 taaaagcata catggtcccg gccgttatcc cgatagaaag accacgatag ccaaaatagg
     4141 tcatcaagac agcataacct agaagtttca acaaactcat ggcaggtctc aaaaagaggg
     4201 catccaaggc tacagaacgg ttggcatagg ccaagtgctc ttggttgatt tcatcaaatt
     4261 ctgcctgcag gcgcttctct tgattaaaag cctgaataat cctgattccc tcgatattct
     4321 ctgccagctt actattgata tctgacaaga gacttctggt tttctcgatg attttcactg
     4381 acttttttcg atagagattg accaaaagga aaatcaaggg gagaaagagc aagactaatg
     4441 ctgtcaaacg aaaatccagc accaacatgg tataaagggt tgtcagaaag ataaaaactg
     4501 ctgagataaa gctggataaa atcccagaaa acatatcact aatcgtctcg gtatcgttgg
     4561 tcaaacgaga aacgatagaa cctgctggcg tcttgtcaaa gtaagacatg cccagtttct
     4621 ccatattggc aaaggcatcc cgacgaatat ccctaacaat actgtaagac acgcgcgcaa
     4681 agagaagatt gccgacatac tgaactacag tttgtaggat gtagagacca tagtagacca
     4741 gcaaaacggt cacggctagt tggttaagat tgctgagata ctggtcgata aagtgggaag
     4801 ccacaagggg aatgacactt ttaatgaccg tcgtcgctag gagaaaactg agtgccaaaa
     4861 aggtaaggag tccataaggc ttgagatagg acatcaagcg cttcaataca gtccattgtt
     4921 cttgtttatt ctgcatcttc ttctcccttc atttccagct gctgagactg gtaggtttgg
     4981 gcataccagc catccaaagc tagcaagtct tcgtgcctgc ctcgttcgat aatttgacca
     5041 ttttgcagga ctaaaatcaa atctgcatgg acaactgcac tcaggcgatg ggcagtgata
     5101 atggttgtct tgtcctttcg cgtctccttg aggttgtcga taatcgcata ctctgtcttg
     5161 gcatctacgg cggataagga atcatccaaa atcaagatat cagggtctaa aatcatagcc
     5221 cgactcatag ccaaccgttg cttttgacca ccagaaagac tgactccttt ttcaccaatc
     5281 agcgtatcaa atccttgagg catgtctaca atatcttggt aaactcgggc tagcttagta
     5341 gcttcctcga ccgctgaaag gggcaagtta ggattgccaa agcggatatt gtctaggatt
     5401 gaagtcgcaa aaagaaactg gtcctgagga acatagccca tgagactgcg aaggtctgtc
     5461 agacgatagt cccgaatatc gtgaccgttt agataaatgg cgcccttatc cacatcgtat
     5521 tcacgcaaga ggagcttgat taaggacgtt ttcccagagc ctgtctgccc aaccaagccc
     5581 agtgtttgcc cttttgccaa actaaagtga atatccgtca gtgtttcctc attttcaaaa
     5641 gcaaagctgt caatggcata ctccaaacgc ccattttcaa taccatccag aggaaactcc
     5701 gggtcttgta caggagattc ctgagacaaa agattttcaa tccgctggta agaaaccttc
     5761 cctcgctgag tagtattaaa gaggaagccg atggccataa gaggccagac cagcatatcc
     5821 aaatagctga taaaggtgac tagattccca actgtaatct gcccttcctg aaccatcaag
     5881 gagccaacca aaagcgttaa aacataggac gaaccaacaa acaagagaac catagggtca
     5941 aagagactat catatttcat ggtttgcagg ttcttttgga aggttaattc attgactgcc
     6001 tgaaaagact tcaactcgtc tgcctgataa ccgaaagact tggtcacttt gatacctgat
     6061 acggactcct gtaccttgtt attgagttca gaaaaagcag tttgggattc gccaaaggcc
     6121 ttatgagtct ttctccctag gcgactagtc gcataggcca tgaaaggtag ggggagaatg
     6181 gcaacaagag tcatctgcca tgagatgcta aagagcatgg tcaacaaagt caccagagcc
     6241 gtgatagagg catccaccgc agacatgaca ccgccacctg ctaaacgagt caaggcattg
     6301 atatcattgg ttgcgtgtgc catcagatca cccgtccgat aggtttgata aaaggctgac
     6361 gacatttttg tgaaatgctt aaacaagcga gaccgcatga tctgtcccaa gcaataagag
     6421 gtcccaagga tatacatacg ccacacatag cgcaaatagt acataccaaa ggctgcaagt
     6481 agcaagtaaa ataggctaag aaggaggtcc tgctgggtta attgccccga tgtgatggca
     6541 tcaatgaccc gccccataac cataggagga atgagattga ggacggaaac caagaccagg
     6601 gccacaatcc cgactagata acggcgtttt tctaacttga aaaaccacca aagtttttga
     6661 ataatggaca taaaatccct ttctgattgc aaatggaagc ctgaggccaa tactcaatga
     6721 aaatcaaaga gcaaactagg aaactagccg caggctgctc aaagcactgc tttgaggttg
     6781 tagatagaac tgacgaagtc agtaacctac atacggcaag gcgacgttga cgcggtttga
     6841 agagattttc gaagagtaca agaccccagg tttttcttat tcataagtta cgactgtaac
     6901 agcacccttg tcatattcag caataaagat attggctaca ttgtcatgcc cttgtttgct
     6961 gaggttatca agcaaccact cttcgctacg accaatcgat tccaagacat ctacttgaat
     7021 cacaccgtca gtcacaactg gatacttagg attttcatct cccatttgca caacgatgag
     7081 ttgcccattt tgctcttgca cagctcgttt gacttgcttc atctggaaaa tcccttggct
     7141 acgaagtttg agggctacat ccgatgcaga caaaccaact gaacgacagg cttatgggtc
     7201 aatattccca tttttgataa ggagagttgg cttaccatca atcaaacgtt tcataaagtg
     7261 aacattgttg ttgaactatt tgagagtcaa gaccagtttg attaaaattt cgatataatt
     7321 aagtgtcatt tgttcacatc caccagttca attgcgtctg tcttatacaa ttcgactttc
     7381 tctaacaggt acttgtctgg ctcacttccg tttagggcac gataatagcg atctcccacc
     7441 ttgataagtg cgccatctgt gtttgtggaa gtattaacat agacttctga cttgtctact
     7501 cccaaatctt tggaaacaac ctcgatgaaa tgaagtgaag tttgaaattt attgttagaa
     7561 acttgattgt tttgataagt cgtgatgctg accaaaagca gagctaaata aggccagagc
     7621 tgaaatcatg accaactcac gaaattcggt tcccttttta tcatggtatg ccttaaaagc
     7681 aaaaaaactg tcacagctaa tagaacaatc ccaaagccaa ccatgatacc attttgctga
     7741 ccgatttggc tgagtacata gtcataagag tagaatttca tgtattttca ctccctttca
     7801 taaaatcatt cttaattata aacgaaagag agtaattttt caaaccatac gttggggaaa
     7861 tagatctttt tttggtataa taaaatctat aatctgaatg aaaaaggtaa ctttatgaaa
     7921 cttatctcat ggaatattga ttccctaaac gctgccctaa ctagtgactc agctcgtgcc
     7981 aaattgtccc aagaagtcct acaaaccttg gtcgctgaaa atgctgatat cattgctatc
     8041 caagaaacca agctttctgc caaaggacct acaaagaaac acgtggaaat tttagaagaa
     8101 ctcttcccag gctacgaaaa cacatggcgc tcttcccaag agcctgcccg taaaggctat
     8161 gctggaacca tgttccttta taagaaagaa cttacgccta cgatcagttt tccagaaatc
     8221 ggtgcccctt ctaccatgga cttggaaggc cgtatcatca ctctagaatt tgatgcattt
     8281 ttcgtaaccc aagtttacac tccaaacgct ggtgacggtc tcaaacgctt ggaagaacgc
     8341 caagtctggg atgttaaata tgctgagtat ttggctgaac tagacaaaga aaaaccagtc
     8401 cttgcgaccg gtgactacaa cgtagcccac aatgaaatcg accttgcaaa tcctgctagc
     8461 aaccgccgtt cacctggatt tactgacgag gaacgtgctg gatttaccaa ccttttggca
     8521 actggattta cagacacctt ccgccatgtt catggcgatg ttcctgaacg ctacacttgg
     8581 tgggcacaac gcagcaaaac ttctaaaatc aacaatacag gctggagaat cgactactgg
     8641 ctcacaagta accgtatcgc tgacaaggtc actaaatctg atatgattga ctcaggtgct
     8701 cgccaagacc atacaccgat tgtcttggaa attgacttgt aaggattttc tatatggact
     8761 acaatgcggt cattcccgag tttcttgtat cgaatatcga acagtcccgg tccttctact
     8821 gtggtttact gggttttagg attgaatact aacgtccaga agaaaacttt ctctttctct
     8881 tgaagagtgt caactaatgt tagaggaggg aacaaaggat caactagctg aactgaccta
     8941 cccctttggt cgtggagtca atctctcctt tggcataaag gatgttccaa aactctatca
     9001 aaaagtgatg gaggcaaact atcctattta tcgtctcttg accaaaagaa agtttcgtgt
     9061 cagtgatccc tatatctatc ctcataaatt tgcagttttg gatccagatg gctatttttt
     9121 aagatttagc gagtagaata ataaaaattg ggaaaagtct ttaaagttag aaaaaggcat
     9181 attgtcaggt gtctaaaaac ttgatagtat gccttttatg atggaaaaat cttctaatac
     9241 tcttcgaaaa tctcttcaag ccacgtcaac gtcgccttac cgtaggtata tgatactgac
     9301 tctgtcagtt ctatccacaa cctcaaaact gtgttttgag caacctgcgg ctagcttcct
     9361 agtttgctct ttgattttca ttgagtataa atgttagctt acaatggagt ttttgttcac
     9421 cctctcttct ttttgttcgc tttttaaacc aaaacactaa caaaatagtt gatttctaaa
     9481 gttatttagt gtaaaataaa aacattggct caagcctatt taaaattgaa tatcgtttta
     9541 cttgtttatg tcgtgaattg gcacgacgtt tctacaaggt gccggaacac ctaacaataa
     9601 gtaagtcagc agtgagatat ggccgttttt gccatgtcta tttaggggac ttacttagag
     9661 attaagtagg tcttttttga atatttttaa tatttccctt aattagtgcg gacgggaggt
     9721 gaaattatcc tgtgaataat ttcaggaatc cagggaattt catccctaat ttttgagccc
     9781 ctagctcttt catttctagg cttaggctaa aatagttccc cgaactattt tactctcaaa
     9841 aatcccgtca tagacaataa caagattatt gtctatgata ccactacaac gggaaatatt
     9901 tgataagact cgcttcgctc gtaatagttt tatcaaatat atagtattag caaaagggat
     9961 cactatagaa atatttaaac aacaagtaaa ttaactttta gactttagga ggtcttatga
    10021 aattattaga agagcgcatc ctcaaggatg ggcatatctt gggtgataac atcctcaagg
    10081 tagattcttt tttaacccac caagttgact ttagcttgat gcgagagatt ggtaaggttt
    10141 ttgcggaaaa atttgctgct actggcatta ccaaggtcgt aaccattgaa gcgtcgggta
    10201 ttgccccagc cgtttttaca gctgaagcct taaacgttcc catgattttc gccaaaaaag
    10261 ctaagaacat caccatgaac gaaggcatct taactgctca agtctactcc tttaccaagc
    10321 aggtgaccag cactgtttct atcgctggaa aattcctctc accagaggac aaggttttga
    10381 ttatcgacga tttccttgct aatggccaag ctgctaaagg cttgattcaa atcatcgaac
    10441 aggccggtgc cacagtccaa gctatcggta tcgtgattga gaaatccttc caagatggtc
    10501 gtgatttgct tgaaaaagca ggctaccctg tcctatcact tgctcgcttg gatcgttttg
    10561 aaaatggtca ggtcgtattt aaggaggcag atctctaatg caaactcaag aaaaacactc
    10621 gcaagcagcc gttctgggct tgcagcactt actagccatg tactcaggat ctatcctggt
    10681 tcccatcatg attgcgacag cccttggcta ttcagctgag cagttgacct acctgatttc
    10741 tacagatatc ttcatgtgtg gggtggcaac cttcctccaa ctccaactca acaaatactt
    10801 tgggattgga ctcccagtcg ttcttggagt tgcattccag tcggtcgctc ccttgattat
    10861 gattgggcaa agccatggta gtggcgctat gtttggtgcc cttatcgcat ctgggattta
    10921 cgtggttctt gtttcaggca tcttctcaaa agtagccaat ctcttcccat ctatcgtaac
    10981 aggatctgtt attaccacga ttggtttaac cttgatccct gtcgctattg gaaatatggg
    11041 aaataacgtt ccagagccaa ctggtcaaag tctcttgctt gcagctatta ctgttctgat
    11101 tatcctcttg atcaacatct ttaccaaagg atttatcaag tctatctcta ttttgattgg
    11161 tctggttgtt ggaactgcca ttgctgctac tatgggcttg gtggacttct ctcctgttgc
    11221 ggtagctcca cttgtccatg tcccaactcc actctacttt gggatgccaa cctttgaaat
    11281 ctcatctatt gtcatgatgt gtatcatcgc aacggtgtct atggttgagt caactggtgt
    11341 ttatctagcc ttgtctgata tcacaaaaga tccaatcgac agcacgcgcc ttcgcaacgg
    11401 ttaccgcgca gaaggtttgg cggtacttct cggaggaatc tttaacacct tcccttacac
    11461 cggattttca caaaacgttg gtttggttaa attgtcaggc atcaaaaaac gcctgccaat
    11521 ctactacgca gctggtttcc tggttctcct tggactgctt cctaagtttg gtgcccttgc
    11581 ccaaatcatt ccaagctccg tcctcggcgg tgccatgctg gtgatgtttg gttttgtatc
    11641 tattcaaggg atgcaaatcc tcgcccgagt tgactttgct aacaatgaac acaacttcct
    11701 tatcgcagct gtttcaatcg ctgcaggtgt cggtctcaac aacagtaatc tctttgtcag
    11761 catgccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtagcca gcctactcgc
    11821 tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg tgcgagcctc
    11881 ttttttgtgt gacaaaataa aaacatctac ctattcatat atgctagtgt catagtcctg
    11941 aaaatcatct gcatcaagaa caatttcaca actcttatac ttttctctta caagtcgttc
    12001 ggcttcatct ggattttcag cctctatact tactaaacgt gataaagttt ctgtaatttc
    12061 tactgtatac tttttcagtt ttagattgct ttccaataat tcacctatta acaataatct
    12121 atgataggag ctatgtagcc actctggcaa tttatctcct acttcccacc catatttttc
    12181 cttcatcaaa gctagttgtt tttcgtcacc aaatttcata ttttcagata accatctcac
    12241 acaatcggga tctgataagg tacagacgat taaacttagg ttatcgtaac ggctttcagt
    12301 ttcataatca ggccagatga cttttaagaa atcatacacc tcttctgatg caacaagttg
    12361 tttcgttttc ataatttccg atactttcct ctacctttaa attctattat ttctttgtct
    12421 cttaatattt gaagctgttg cctaatcttt tctttgatat gattgttctt aacaaagata
    12481 ttttttaggt cactttcaaa acgatacata tcttcaaggg taaattctga accctctatc
    12541 ttatctatac aatttagaat ttctattgtc caacctcttg atgacagaga gctcttcctt
    12601 aaaaataaac cttgcttaaa ttcttttgta actttttctg gatctctaac ttgtccatct
    12661 tgcacaagaa atatccttcc tttagaaggt acttgtgata aatcaatgtt acaaccaatc
    12721 caacctgctc gtctagcagt tggtgcaagt ggttttcttt gaataattga tttcggtgta
    12781 acaaattgct tcggaaggac aagaaagtta tttacctcaa aattttttgt gtaagttaaa
    12841 aaaaagaaat taggattatt atctgcctgc acacgcttca tcatcgttgc ataagcacca
    12901 tcattgattg ttgatgaaaa atttcctttt ttgctcttta gttcaaactc ctcactacaa
    12961 tgattacagt aaaaatctgc tacaggccga ttattttcaa aatgatttaa gggattgttc
    13021 ccacaattag ggcaataaga ctgacgataa acccaatcct ccgttaagat acgtgccttt
    13081 tggctattac ttttataggt ttctactaat tctaaattaa agtgtaattc catttaaaaa
    13141 ttcctcacta atatgagaac tcactcgata taagcaatta aaat
//
LOCUS       DAVKGC010000049        13162 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKGC010000049 DAVKGC010000000
VERSION     DAVKGC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.049"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..242
                     /locus_tag="VW049_001197"
     CDS             <1..242
                     /locus_tag="VW049_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9432329.1"
                     /translation="AEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNSSSSQTNI
                     LAQLMDFFTGNGHLEDLSKISEPEALYAYIAEATA"
     gene            280..581
                     /locus_tag="VW049_001198"
                     /pseudo
     CDS             280..581
                     /locus_tag="VW049_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            589..1392
                     /locus_tag="VW049_001199"
                     /pseudo
     CDS             589..1392
                     /locus_tag="VW049_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1583..3874
                     /gene="pcrA"
                     /locus_tag="VW049_001200"
     CDS             1583..3874
                     /gene="pcrA"
                     /locus_tag="VW049_001200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9432330.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDSDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            3917..4597
                     /gene="radC"
                     /locus_tag="VW049_001201"
     CDS             3917..4597
                     /gene="radC"
                     /locus_tag="VW049_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9432331.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(4594..5283)
                     /locus_tag="VW049_001202"
     CDS             complement(4594..5283)
                     /locus_tag="VW049_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9432332.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(5301..5942)
                     /locus_tag="VW049_001203"
     CDS             complement(5301..5942)
                     /locus_tag="VW049_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9432333.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(6025..6240)
                     /locus_tag="VW049_001204"
     CDS             complement(6025..6240)
                     /locus_tag="VW049_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9432334.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(6248..6595)
                     /locus_tag="VW049_001205"
     CDS             complement(6248..6595)
                     /locus_tag="VW049_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9432335.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(6600..7715)
                     /locus_tag="VW049_001206"
     CDS             complement(6600..7715)
                     /locus_tag="VW049_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9432336.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(7725..8684)
                     /locus_tag="VW049_001207"
     CDS             complement(7725..8684)
                     /locus_tag="VW049_001207"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9432337.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(8797..9366)
                     /locus_tag="VW049_001208"
     CDS             complement(8797..9366)
                     /locus_tag="VW049_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9432338.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            9494..10165
                     /locus_tag="VW049_001209"
     CDS             9494..10165
                     /locus_tag="VW049_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9432339.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            10149..10967
                     /locus_tag="VW049_001210"
     CDS             10149..10967
                     /locus_tag="VW049_001210"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9432340.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            10964..11860
                     /locus_tag="VW049_001211"
     CDS             10964..11860
                     /locus_tag="VW049_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9432341.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            11904..12878
                     /gene="pta"
                     /locus_tag="VW049_001212"
     CDS             11904..12878
                     /gene="pta"
                     /locus_tag="VW049_001212"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9432342.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3899 a         2510 c         2816 g         3937 t
ORIGIN      
        1 tagctgaaaa agtggtggtt gttcgattag aaaaaccagt aacttttcac aatatgatag
       61 ctcctgataa ggaagtagaa gtatccctac tcttctttat cattaacaat tcaagttcaa
      121 gtcaaacaaa tattctggct cagttgatgg actttttcac aggaaatgga catcttgaag
      181 atttatcaaa aatttctgaa ccagaagccc tttatgctta cattgctgaa gcaaccgctt
      241 aatcttgtct attaaaaaat aaaatcggag gaaatccaaa tgattaaaat tcttgctgcc
      301 tgcggtgagg tgttaactcg agttaccaaa ttaaaagtgc tctagaagaa gaactttcaa
      361 accgtggtta tgatgttcac tgtgatgcag tcatggtgaa agatgtaaac gaagacctta
      421 tgaaaggtta cgatatcttt acaccaatcg ctgcaacaga tcttggtttt gaaccaggta
      481 tcccagttat cgaagctggg ccaatcttat tccgtatacc agcaatgagc gctccagtat
      541 ttgacaatat tagactccct gcgaaacaaa acatggtata gtagttctat gaatgatgaa
      601 gcaagtaaac aactaactga tgcacgattt aagcgttttg ttggtgttca gcgcacgact
      661 tttgaagaga tgttagctgt attaaaaaca gcttatcaac ttaaacacgc aaaaggtgga
      721 cgaaaaccta aattaagcct agaagacctt cttatgccca ctcttcaata gtgcgagaat
      781 atcgaactta tgaagaaatt gcggctgatt ttggtattca cgaaagcaac tttatccgtc
      841 ggagccaatg ggttgaaata actcttgttc aaagtggttt tacggtttca agaactcctc
      901 tcagttctga ggacacggta atgattgatg cgacggaagt aaaaatcaat cgccctaaaa
      961 aaacaattag cgaatgattc tggtaaaaag aaatttcacg ctatgaaggc tcaagcgatt
     1021 gtcacaagtc aagggagaat tgtttctttg gatatcgctg tgaactatag tcatgatatg
     1081 aagttgttca aaatgagtcg tagaaatatc gaacaagctg gtaaaatctt ggctgacagt
     1141 ggttatcaag ggctcatgaa gatatatcct caagcacaaa ctccacgtaa atccagcaaa
     1201 ctcaagccgc taacagctga agataaagcc tataaccatg cgctatctaa ggaaagaagc
     1261 aaggttgaga acatctttgc caaagtaaaa acgtttaaaa tattttcaac aacctatcga
     1321 aatcatcgta aacgcttcgg attacgaatg aatttgagtg ctggtattat caatcatgaa
     1381 ctaggattct agttttgcag gaagtctatt gaggtattga gctagtttat gaaaaaattg
     1441 ggtgaaaagt cgagtgtttt agaaacccac agtgtagtat tctagtttca atccactata
     1501 ttttgctact ccccgtaaag tttctatttt ccctgatttc tgatataata gaaatattga
     1561 cttcaagagt aaggaagaga agatgaacgc attattaaat ggaatgaatg accgtcaggc
     1621 tgaggcggtg caaacgacag aaggtccctt gctaatcatg gcaggggctg gttctggaaa
     1681 gactcgtgtt ttgacccacc gtatcgctta tttgattgat gaaaagctgg tcaatccttg
     1741 gaatatcttg gccattacct ttaccaacaa ggctgcgcgt gagatgaaag agcgtgctta
     1801 tagcctcaat ccagcgactc aggactgtct gattgcgacc ttccactcca tgtgtgtgcg
     1861 tattttgcgt cgcgatgcgg accatattgg ctacaatcgt aattttacaa ttgtggatcc
     1921 tggtgaacag cgaacgctca tgaaacgtat tctcaaacag ttgaacttgg accctaaaaa
     1981 atggaatgaa cgaactattt tggggaccat ttccaatgct aagaatgatt tgattgatga
     2041 tgttgcttat gctgcccaag ctggcgatat gtatacgcaa attgtggccc agtgttatac
     2101 agcctatcaa aaagaacttc gtcagtctga atccgttgac tttgatgatt tgattatgct
     2161 gaccttgcgt ctctttgatc aaaatcctga tgttttgacc tactaccagc aaaaattcca
     2221 atacatccac gttgatgagt accaagatac caaccacgct cagtaccaat tggtcaaact
     2281 cttggcttcc cgttttaaaa atatctgtgt ggttggggat gcggaccagt ctatctacgg
     2341 ttggcgtggt gctgatatgc agaatatctt ggactttgaa aaggattacc ccaaagccaa
     2401 ggttgttttg ttggaggaaa attaccgctc aaccaaaacc attctccaag cggccaacga
     2461 ggttattaaa aataataaaa atcgccgtcc taaaaatctc tggactcaaa acgctgatgg
     2521 ggagcaaatc gtttactatc gtgccgatga tgagctggat gaggctgtat ttgtagccag
     2581 aaccatcgat gaacttagtc gcagtcaaaa cttccttcat aaggattttg cagttctcta
     2641 tcggactaat gcccagtccc gtacaattga ggaagccctg ctcaagtcta acattcctta
     2701 taccatggtt ggcggaacca aattctacag ccgtaaggaa attcgcgata ttattgctta
     2761 tctcaacctt attgctaatt tgagtgacaa tattagtttt gagcgtatta tcaacgagcc
     2821 taaacgtgga attggtctag gtacagttga gaaaatccgt gattttgcaa atttgcaaaa
     2881 tatgtctatg ctggatgctt ctgctaatat tatgttgtct ggtatcaagg gtaaggcagc
     2941 ccaatctatc tgggattttg ccaatatgat gcttgatttg cgggagcagc tagaccactt
     3001 aagcattaca gagttggttg agtccgtcct agaaaaaaca ggttatgtcg atattcttaa
     3061 cgcccaagcg actctagaaa gcaaggcacg ggttgaaaat atcgaagagt ttctttctgt
     3121 tacgaagaac tttgatgaca ccacggatgt gacagaagag gaaactggtc tggacaaact
     3181 gagtcgtttc ttaaatgact tggctttgat tgccgacaca gattcaggta gtcaggagac
     3241 atcagaagtg accttgatga ccctgcatgc tgccaaaggt ctcgaatttc cagttgtctt
     3301 tttgattggg atggaagaaa atgtctttcc acttagtcgt gcgactgaag attcagatga
     3361 attagaagaa gagcgccgtc tagcctatgt aggtatcacg cgtgcagaga aaattctcta
     3421 tctgaccaat gccaactcac gcttgctttt tggtcgtacc aattataacc gtccgactcg
     3481 ttttattaac gaaatcagtt cagacttgct tgagtatcaa ggtctggctc gtcctgcaaa
     3541 tacaagcttt aaggcatcat atagcagtgg tagtatttcc tttggtcaag gtatgagttt
     3601 ggctcaggct cttcaagacc gtaaacgcgg tgctgcccca aaatcaatcc agtcaagcgg
     3661 tcttccattt ggtcaattta cagctggcgc aaaaccagca tctagcgagg caaattggtc
     3721 cattggtgat attgctctcc acaagaaatg gggagaggga accgttctgg aagtttcagg
     3781 tagcggtgct aggcaggaat tgaaaatcaa tttcccagaa gtaggtttga aaaaactttt
     3841 agccagtgtg gctccaattg agaaaaaaat ctaattttcc atccttctca cgaataataa
     3901 agtgaggagg atttttatgt acagtatttc attccaagaa gattcactat taccaagaga
     3961 aaggctggcc aaggaaggag ttgaagcgct tagtaaccaa gagttgctag ctattttact
     4021 caggacagga acacgtcaag ctagcgtttt tgaaattgcc caaaaagtct tgaacaatct
     4081 ttcaagccta acggatttga aaaaaatgac cctgcaggaa ttgcagagtt tgtctggtat
     4141 tgggcgtgtt aaggccatag aattacaagc tatgattgaa ctggggcatc gtattcacaa
     4201 acacgagact cttgaaatgg aaagtattct cagcagtcaa aagttggcca agaagatgca
     4261 gcaggaatta ggggataaaa aacaagagca cctggtggca ctctatctca atactcaaaa
     4321 tcaaatcatc catcagcaga ccatttttat cgggtctgta actcgtagta tcgctgaacc
     4381 gcgagagatt cttcactatg caatcaagca tatggcgact tctcttatct tggtccacaa
     4441 tcatccttca ggagcggtag cgcctagcca aaatgatgat catgtcacta aacttgttaa
     4501 agaagcctgc gaattgatgg ggattgttct cttggaccat ttgattgtct ctcattctaa
     4561 ttactttagt tatcgtgaaa agacagattt aatctaaagt tcattaacga catagtcaaa
     4621 gagtttttta tctttgggac gattttcaaa aagaagttct ggatgccatt ggacaccgag
     4681 aaaggcgaca tcatccgtac tcatgacagc ctcaatgata ccatctttag gatcatgagc
     4741 cacaactttt aaatttggtg ctaagtcctt gatgctctgg tggtggaagg agttgatatg
     4801 agagatttct ccatagattt cttggagaac ggtatctggt tctgttacca agcgttgagt
     4861 tgtgtactca acagaagaat cctgccaatg gtcttcgata tcttggtaca aagttccacc
     4921 catggcaacg ttaaagagtt gggtaccacg gcagacagag aaaatgggct ttttctgttt
     4981 aatagcttcc ttgatgaggg ccagttcgaa gatatctctt tgaaggtgat agtcatcact
     5041 atcaatggtt ttgggttcgc cataaaattt tggatcgaca ttttgcccac ctgtcaagat
     5101 gagcttgtca atcaaactga tatagtggca ggccatttct tgatcaccaa tcggtaggat
     5161 gatgggaatc cctccagcat ctttaacgcc ttcaacaaag ccttttgctg cgtagctcat
     5221 catgatgtca tcatctggat gagttttttc gtttcctgta atcccaataa ctggtttttt
     5281 cataaaatga ttttcgcttt ctaatcctct tttcgcatga agtagaggag ggtttggagt
     5341 tcacttgtca aatcgacata ctgaacgacc acgtcttttg gtaaatgcag atggactggt
     5401 gaaaaactga gaattccttt cacaccagca tcaaccaaga gattagcaac ctcttgtgac
     5461 ttgacgctgg gaacagttag gatagcagtc ttcacatcag catccttgat tttatccttg
     5521 atctgagaaa tcccgtaaat gggaatcccg tcaggagttt gggtaccgac ttcaggatgg
     5581 tcgtctaggt caaaggccat gataatcttc atcttgttac gttcgtggaa gcggtagtgg
     5641 agaagggcat ggcccatatt tccaatacca accagcatga cattggtaat agagttgtca
     5701 ttgagcaaat cggcaaaaaa tgtcattagt tttttgacat catagccaaa accacgacga
     5761 ccaagttcac caaaatagga aaaatcacga cgtacggtcg ctgaatcaat accgatagcc
     5821 tctgcaattt gcttagagtt ggcacgttca atcttttctg catgaaatct cttaaaaatt
     5881 cgatagtaga gagagagtct ttttgctgta gcttttggaa tagcaaactg tttatctttc
     5941 acaaaatcac aacctttcta ttcttctatt ttatagaaac attgtgaaaa aatcaacaaa
     6001 aataagaaaa aactaagaaa aatcttagtt ttgatgtaaa aaatctgcat gagatagaaa
     6061 acggtagagg tctccgacca gcccctgata aacttttttg cccctaaaag tcagagaagt
     6121 cacataaagt gtatctggta aggttacaca tcctgacaaa gtcaacatga gagcctcatg
     6181 atcctcatac ttgagagtac gctctacatg atagcagtcc ttataggtca gttcaaacat
     6241 tttggctcta tctttccgat tttgtaaaga caccacgttc taccaagcta tccatgagga
     6301 agtagaattt ttcctgatga atatggtggt cttctgattt gaaaatatca actagacgaa
     6361 ggccaaactt gtcagtgata ttgattttag cccctgtaag ttccttgtta atgatgattt
     6421 tgagttggaa gccttcaccg ctgtttggca ctttttccaa aaggcgagtc agttcatagt
     6481 taccaacctt agtttcaaaa aaggtgttat ctttgagggt gaatttttta acagaagggc
     6541 taagagtgta atcgtaacga caatttttta actgaatgat tttttcaaat gccatatggc
     6601 taacctccga taatttcttt taaggttttt gcgagggttt gtaggtcttc aacggtattt
     6661 tgtggcgaca aactgatgcg aagggattcc ttcaagcgtt ctgaatttgc gccatacatg
     6721 gctttaagaa catggctgga ttggacaacg cctgcagtac aggctgagcc agtagagatt
     6781 gaaattccag ctaaatctag ccgaaggagt aagaggtcat ttttctgacc aggaaatcca
     6841 atattgagaa cataagggag atgatgtttt cctctattca agtaatactg aatgccctcc
     6901 agctctgcca gaaaggcagt ttctagattt tgtacatgtt gaaaatgttc ttcttgtttt
     6961 tctaggtctt cttttagggc tgcaaccatg cctacgatgg caggcagatt ttcagttcct
     7021 gcacgttttt tctgttcctg atctccgcca tgtagatagg aatcaaagtc catgctagat
     7081 gcgtagagaa aaccgattcc cttaggacca tggaatttgt gggcagaagc agtgagaaaa
     7141 tcaatgccca attcttctga atgaattggg attttaccaa tagcctgaac tgcatcaaca
     7201 tgataggcag cagggtgttg cttgagtatt tggccaattt cagcgatggg aagtaggttt
     7261 cctgtctcat tattggcaaa catagtagaa accaaaatcg tatcgtcacg taaagccttt
     7321 tgaatttgct gggctgtgat ttcttgattt tctggctgga taatggttgc ttcaaaccca
     7381 aagtgttgaa ccaagtaatc aattgtttca aggacagcat ggtgctcgat ggcagttgtg
     7441 atgatatgtt ttccttgttc ttggtgacga agacagtagc caatgatggt agtattattg
     7501 ccttcagtcc caccagaagt gaaaaagata tgttgaggtt ttgtccttag taactgggct
     7561 agttcctgac gggcttctcg caagagtttg ccagcttgac gaccatgacc atgaatacta
     7621 gaaggatttc cgtgggtttc ttgcataacc ttggtcatag ctgaaatagc aactgctgac
     7681 ataggagtcg ttgcagcatt gtccaaataa atcaaagaat caccttattt ctttttattg
     7741 taggcaaaga gtgggctgac tggttttctt tcgtgaatac ggacgatagc atcaccaatt
     7801 aactcactag cagtgatgta gcatacattt ttaggagttt tttcttttgt tgctactgaa
     7861 tcagtcacaa gaatttcttt aatattagta ttgtcaagaa gctcagcagc tccctcgacg
     7921 aagagaccgt ggctagaaac agcataaatt tctgtagctc cttcacgttc aacgatttta
     7981 gaagcttcag agaaggtacg tcctgtattt aaaatatcat caatcaagat agctttctta
     8041 ccttcaacat caccaataat ataaccttcg ttacgagttg catcgtcttg agggtagtcg
     8101 ataatggcga taggagcatc aagatattca gccaggctac gcgcacgttt gacacctgaa
     8161 tttttagggc taacgacaac aacatctgaa ccaagcaatc ctttatcgca gtaatgtttt
     8221 gcgaataggg gaacagtgaa aagattatcc actggaatat caaagaaacc ttgaacctga
     8281 acggcatgca aatcaagagt caggatacga tcaactccag ccttaaccag catattggca
     8341 actagttttg ctgtaagtgg ctcacgagga caagcaatgc ggtcttgacg tgcatagcca
     8401 aaatatggaa ggacaacgtt gatactgtgg gcacttgcac gcacacaagc atcgaccatg
     8461 attaacaatt ccattaggtg gttgttgaca gggaaacttg ttgattggat gatgtaaaca
     8521 tcataaccac ggacactttc ttcgatattt acttggattt ctccgtctga aaattgacgt
     8581 gatgatagtt ttccaagtgg gacaccaaca gcttgggcaa ttttttgtgc aatctcttgg
     8641 ttagagttga gtgcgaaaag tttcatgttt tttctatctg acattataga ccgtcctctg
     8701 taaactttat aaatcctagt tatatttacc ttacatatat gaactgggat ttgtgtattt
     8761 ttatcttttc tattttacca aaaaatggag attatttcag ctatttttca tacttttgac
     8821 aaatcgaacc aattttgaag gagctttttg gtaggaaatc tgatttttct ctaaaaattg
     8881 tcggaaatcc tgtttgcctt gctcatgatt ttccacttca agctccaatt cgtaatctgt
     8941 tatatcaaag tatcggctct gatccagtgc catgagacca atagctgttt tcatttcata
     9001 gcgaagcgtt gttagacaac caagaacctg ccagttctta ctttggatac catgtttcgc
     9061 caattcatcc agtactagcc cttgaggaag ttcttcctta ctcagatagt tctcagcatc
     9121 ttttagttgc aatttttggt tgtattccat gtttccaaca ctctgcggga ctttgagtgt
     9181 caactcagcc cagtcttcaa aggttcgaat gcgcatagcg actttctttt ctcgcagttc
     9241 aaaatcaggc gtgtcgatgt agtaatttgt ttgaagaaca ggagtgacac ctgtgaactg
     9301 gtcttttaga cgattgtatt catctttttt caatagtgtt ttcaattcaa tttctaaatg
     9361 tttcattttt cttacctttt tttatcgttg aaagcggatt tatggtataa taagcattgt
     9421 atttattgta tatgaatctg gagaaaaaat caaagatatt tttgacggat aatatgagaa
     9481 caagggagaa tatatgacct tagaatggga agaatttcta gatccttaca ttcaagctgt
     9541 tggtgagtta aagattaaac ttcgtggtat tcgtaagcaa tatcgtaagc aaaataagca
     9601 ttctccaatt gagtttgtga ccggtcgagt taagccaatt gagagcatca aagaaaaaat
     9661 ggctcgtcgt ggcattactt atgcgacctt ggaacacgat ttgcaggata ttgctggctt
     9721 acgtgtgatg gttcagtttg tagatgacgt caaggaagta gtggatattt tgcacaagcg
     9781 tcaggatatg cgaatcatac aggagcgaga ttacattact catagaaaag catcaggcta
     9841 tcgttcctat catgtggtag tagaatatac ggttgatacc atcaatggag ttaagactat
     9901 tttggcagaa attcaaattc gtactttggc catgaatttc tgggcaacga tagaacattc
     9961 tctcaactac aagtaccaag gggatttccc agatgagatt aagaagcgac tggaaattac
    10021 agctagaatc gcccatcagt tggatgaaga aatgggtgaa attcgtgatg atatccaaga
    10081 agctcaggca ctttttgatc ctttgagtag aaaattaaat gacggtgtag gaaacagtga
    10141 cgatacagat gaagaataca ggtaaacgaa ttgatctgat agccaataga aaaccgcaga
    10201 gtcaaagggt tttgtatgaa ttgcgagatc gtttgaagag aaatcagttt atactcaatg
    10261 ataccaatcc ggatattgtc atttccattg gcggggatgg tatgctcttg tcggcctttc
    10321 ataagtacga aaatcagctt gagaaggtcc gctttatcgg tcttcacact ggacatttgg
    10381 gcttctatac agattatcgt gattttgagt tggacaagct agtgactaat ttgcagctag
    10441 atactggggc aagggtttct taccctgttc tgaatgtgaa ggtctttctt gaaaatggtg
    10501 aagttaagat tttcagagca ctcaacgaag ccagcatccg caggtctgat cgaaccatgg
    10561 tggcagatat tgtaataaat ggtgttccct ttgaacgttt tcgtggagac gggctaacag
    10621 tttcgacacc gactggtagt actgcctata acaagtctct tggcggtgct gttttacacc
    10681 ctaccattga agctttgcaa ttaacggaaa ttgccagcct taataatcgt gtctatcgaa
    10741 cactgggctc ttccattatt gtgcctaaga aggataagat tgaacttatt ccaacaagaa
    10801 acgattatca tactatttcg gttgacaata gcgtttattc tttccgtaat attgagcgta
    10861 ttgagtatca aatcgcccat cataagattc actttgtcgc gactcctagc cataccagtt
    10921 tctggaaccg tgttaaggat gcctttatcg gtgaggtgga tgaatgaggt ttgaatttat
    10981 cgcagatgaa catgtcaagg ttaagacctt cttaaaaaag cacgaggttt ctaagggatt
    11041 gctggccaag attaagtttc gaggtggagc tattctggtc aataatcaac cgcaaaatgc
    11101 aacgtatcta ttggacgttg gagactacgt taccattgac attcccgctg agaaaggctt
    11161 tgaaaccttg gaggctattg agcttccatt agatattctc tatgaggatg accactttct
    11221 agtcttgaat aaaccctatg gagtggcttc tattcctagt gttaatcact ctaataccat
    11281 tgccaatttt atcaagggtt actatgtcaa gcaaaattat gaaaatcagc aggttcacat
    11341 tgttaccaga ctagataggg acacttctgg cttgatgctc tttgccaagc acggttatgc
    11401 ccatgcacga ttagacaagc agttgcagaa gaaatctatc gagaaacgct actttgcttt
    11461 ggttaaggga gatggacatt tggaaccaga aggggaaatt attgctccga ttgcgcgtga
    11521 tgaagattcc attattacca gacgagtggc taaaggcgga aagtatgccc atacttcata
    11581 caagattgta gcttcttatg gaaatattca cttggtctat attcacctgc acactggtcg
    11641 aacccatcaa atccgagtcc atttttctca tatcggtttt cctttgctgg gagatgattt
    11701 gtatggtggt agtctggaag atggtattca acgtcaggct ctgcattgcc attacctatc
    11761 cttttatcat ccatttttag agcaagactt gcagttagaa agtcccttgc cggatgattt
    11821 cagtaacctt attacccagt tatcaactaa tactctataa aaactgtctc agagtataat
    11881 tattatctta aaggagaaaa ctcatggaag tttttgaaag tctcaaagcc aaccttgttg
    11941 gtaaaaatgc tcgtatcgtt ctccctgaag gggaagagcc tcgtattctt caagcaacaa
    12001 aacgcttagt aaaagaaaca gaagtgattc ctgttttgct tggaaatcct gaaaaaatta
    12061 aaatttatct tgaaattgaa ggaatcatgg atggttatga ggtcatcgac cctcaacatt
    12121 atcctcaatt tgaagaaatg gtttctgcct tggtggagcg tcgcaagggc aaaatgactg
    12181 aagaagatgt acgcaaggtt ttggttgaag atgtcaacta ctttggtgtg atgttggttt
    12241 acttgggctt ggttgatgga atggtgtcag gagcgattca ctcaacagct tcaacagttc
    12301 gcccagctct acaaatcatc aaaactcgtc caaatgtaac tcgtacttca ggagccttcc
    12361 tcatggttcg tggtacggaa cgttacctat ttggagactg tgccattaat atcaatccag
    12421 atgcagaagc cttggctgaa attgccatca actcagcaat cacagctaag atgtttggca
    12481 tcgaacctaa aattgccatg ttgagctatt ctactaaagg ttcagggttt ggtgaaagcg
    12541 ttgataaggt cgttgaagca actaaaattg ctcacgactt gcgtcctgac cttgaaatcg
    12601 atggtgagtt gcaatttgat gcggcctttg ttcccgaaac tgcagctctg aaagctccgg
    12661 gaagtacagt agctggtcaa gcaaatgtct tcatcttccc aggtatcgag gcaggaaata
    12721 tcggttacaa gatggctgaa cgcctgggtg gctttgcggc tgtaggacct gttttgcaag
    12781 gtttaaacaa gccagttaat gatctttctc gtggatgtaa tgcagatgat gtttacaagt
    12841 tgaccctcat cacagcagct caagcagttc atcaatagtg aaaactataa agtgatatac
    12901 tatgctatac tgtagttatg aaactatgta cgaaaagcac tgccattaat tcctgagaac
    12961 taaattactg attggtgtca aaaaggaaaa cttccaagcg atgatatcct gtctatacac
    13021 gacctataga aatctgtaat atacatatcc gtaaaacgat aaattccctt tttgatttta
    13081 aatgagtatg aaaagagaat tttttggctc tttgtcaact gtagtgggtt gaagtcagct
    13141 aagctcgaga aaggacaaat tt
//
LOCUS       DAVKGC010000050        13116 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKGC010000050 DAVKGC010000000
VERSION     DAVKGC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.050"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(1..699)
                     /locus_tag="VW049_001213"
     CDS             complement(1..699)
                     /locus_tag="VW049_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432343.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            complement(709..1461)
                     /locus_tag="VW049_001214"
     CDS             complement(709..1461)
                     /locus_tag="VW049_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432344.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(1461..2225)
                     /locus_tag="VW049_001215"
     CDS             complement(1461..2225)
                     /locus_tag="VW049_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432345.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSAHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(2321..2724)
                     /locus_tag="VW049_001216"
                     /pseudo
     CDS             complement(2321..2724)
                     /locus_tag="VW049_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2904..3536)
                     /locus_tag="VW049_001217"
     CDS             complement(2904..3536)
                     /locus_tag="VW049_001217"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9432346.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPGTRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFTDAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(3570..4271)
                     /gene="pyrF"
                     /locus_tag="VW049_001218"
     CDS             complement(3570..4271)
                     /gene="pyrF"
                     /locus_tag="VW049_001218"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9432347.1"
                     /translation="MREHRSIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(4504..5703)
                     /gene="divIB"
                     /locus_tag="VW049_001219"
     CDS             complement(4504..5703)
                     /gene="divIB"
                     /locus_tag="VW049_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9432348.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEIEESES
                     KEKEEQDKKLAKKAIKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(5713..6771)
                     /locus_tag="VW049_001220"
     CDS             complement(5713..6771)
                     /locus_tag="VW049_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9432349.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(6775..8127)
                     /locus_tag="VW049_001221"
     CDS             complement(6775..8127)
                     /locus_tag="VW049_001221"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9432350.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(8231..8872)
                     /locus_tag="VW049_001222"
     CDS             complement(8231..8872)
                     /locus_tag="VW049_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432351.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(8874..10292)
                     /locus_tag="VW049_001223"
                     /pseudo
     CDS             complement(8874..10292)
                     /locus_tag="VW049_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            10459..11805
                     /locus_tag="VW049_001224"
     CDS             10459..11805
                     /locus_tag="VW049_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEU9432352.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADVYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDENLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFFKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPGRKASLMMH"
     gene            complement(11968..12603)
                     /locus_tag="VW049_001225"
     CDS             complement(11968..12603)
                     /locus_tag="VW049_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9432353.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGDSLSQIIHTSLN"
     gene            complement(12674..12973)
                     /locus_tag="VW049_001226"
                     /pseudo
     CDS             complement(12674..12973)
                     /locus_tag="VW049_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         4098 a         2820 c         2293 g         3905 t
ORIGIN      
        1 tcattctata aacctcctct tttgacgagt gaaatagaaa atacttttaa aaagaaagac
       61 taaaaatagc aactgaagaa ataaatctcg tcctatatct ccattccctc gattcaaaat
      121 ataaaataga taattagttc gattccctac aaatagacca ccaaacacaa tcatgagtaa
      181 aaagaaacta acgcaagcaa agttcatttg tacattcgct cctagtgcgt ataacataaa
      241 gcaaagcaag gatagcacac aagcattcaa agaatcaact ataaacaaaa ctcctatatc
      301 ccagtttggt aactgtaaca taaattgaat cactagaact tttaccatga ttaaaatgag
      361 tccataaaga aaacaggtcc aaatctgata cctaatccag agcttaatag aagaaaatcg
      421 aatcacttcc ataaagaaat cggatgctct tctacgatat acaactgcta gaaaaaatgt
      481 atgaataata aaccaatgaa gcaaccatat ggataagact aaaaaagagt tttgactcag
      541 attaggccct tttaaaattt ctataacaag tctatcattt ccttctaacc aataccataa
      601 tcctaaacaa atcaatatcc aaaaaagaac ataaccccaa aagtacttgg tatagattat
      661 ctgattcaac ctccacatgt gactacttaa ctttcccatt tccaactttt acactctcct
      721 ccagtttcta tgaacagaaa atacagtcaa tggaattatg atagatagta atgtaagcca
      781 ataaatcaat gcatacggat tggaatttac catatactga aaaattggca ataatccaat
      841 tatcccattg tccaactgat aaatccagaa taaggattct acaaataaaa aagtaaacag
      901 aaatactaag ctcatgtttg attttttaaa ccaagaaaaa ctgctaatag taagtaagga
      961 taaaaagaaa tagtatgcta tataagagaa aaaaaatcct ataaagaaac tagcattgtt
     1021 tgcaatactt ataccataaa attctcttaa aaaatcaacc tcctttatct ccaaagagaa
     1081 gctaaaacca ttactaaatg caattagaaa aatcaataaa aataaagtcg ccgtccaaat
     1141 ccccgtacta agagctgcta atttgaaact aaaactggta aagtgcttaa ttgatttcag
     1201 acgaatgcga cactccaacc tattaaaata gttattcatc aaataaaaaa agaacaatat
     1261 atatgtgaac ggaaagcaat atactccagt cgtcatatct tgaagtaaaa ctaagatcca
     1321 ttctaataca tttggatgga ttgaatattg gcgacagcgc aataaatata ctgtactaga
     1381 taaaacacag gatagcagta atataaaata aaccaatact gctaagaaat cttttttata
     1441 aaattgaaca aattgtttca ttatacatag tcctctgaat gtagaaaaaa tgtaccataa
     1501 acaaccaaac aactaacaaa taaaataaaa gcaagatgag cactaactaa ggaaagactg
     1561 atatctttct gatatcccaa agctagcgtt gtcacaggtt ctaagtaaga tagccctaaa
     1621 atagcccata aaataccacc aaccatcata taggcaactg ggatgaaaat agctcctatt
     1681 tttttcttca ctagcaaagc actagctagt ccaaaaatag agaacacagc gccccaaact
     1741 ccataccaga gagtcgtcac aagactatag agcaactgat tggaatcaaa taattctttt
     1801 aaggcaccac tataatctcc aatataaatt tcctgataag gagtcactaa aagattaatt
     1861 cctaataata ataaataggg gagaaaaaag actagaaaag aagaaataat tgcagtacta
     1921 cccacaatag ccagatactt ctttttagaa attcgacaca attgtgctgt tagaaaatga
     1981 ctctcagcat cctctattat ctgactagaa tagggcagtg tacagataag tgcagctact
     2041 aggctaatcg gtgaaaatcc tcgaatagaa gaagcggcaa aaaaaatcga taaaccttca
     2101 attttataaa taccattgaa agcaaggaaa tttcctaaac tcatgcaaag aagggtcaaa
     2161 aataaagaca tataaaatcg aggtgattga acgactcgta caagattaca aatgaaaaat
     2221 tccatcctta ctcctcctta taataaaaat agggtgtagc attctttttt catgctacac
     2281 ccacaatcaa ccatctctaa ggcttactct gacaagtaag ttaataagaa tctggattcc
     2341 aagaacctga agtatgaatt cttacatgat ttccaaattg tggcgccata gctaatctag
     2401 taccagaacc aatataattg tcaccacctc cattatagta catgacaatc ctagagccag
     2461 accccaatga atataccggg gtaatatctg acccactata ggcgctacga atagaggtat
     2521 ttaacctttt accgccacca gtgctgtcat tattattaat tccagcagag gcgttttctt
     2581 tctcaagctc actagttgta tccgaacccc aacgcggcga cgttaaatca taatctccaa
     2641 tctgtctagc taaaactgta gggagaaaca aactagatgc aattgtaact acgcaaaaaa
     2701 ggaatatatt tttcttcttt tcatgacatc gtcctcttaa gaagtgataa acaagatagt
     2761 tgattgatca atttgataca tattacattt tcattatatc aataaaagaa agcgttgtca
     2821 attattttat ttaaaatatt gtcatatttt aaataaaatg gtaaaagaga ctcggtagcc
     2881 tcgtctctct ttatcttact gacctaacct tcttgccaat tttcttggtc ttctttaaag
     2941 cgttttagaa gatcaaggcc ctctggcgtg atgtaacctt cttcttgggc tagatggata
     3001 agctcgctat agtttgaaag cgtcacaagt ttaacaccag catctgtaaa gttcttatct
     3061 gcttttggca attggtagct gaaaatcgct acaactccaa gtacatctgc tccttctcgc
     3121 ttggctgctg ctacagcttc aagaactgaa ccacccgttg aaataaggtc ttcaaccact
     3181 accatttttt gaccttgagc tacgcgacct tcgatttgat taccagctcc gtggtctttt
     3241 ggtttgctac ggatgtaggc aaaaggcaag tccatcttat cagcaataat ggctccgtgt
     3301 ggaatccctg ctgttgcagt tcctgcaatc acttctactt caggaaaggc ttctttgata
     3361 gcttccacaa aaccattttc aattagggta cgagttcctg gataggctag tgtcacacga
     3421 ttatcagtgt aaatcggtga cttgatacca gatgcccaag tgaagggttc ctctggtttg
     3481 aggtaaacgg cttggatttt caagaggtgg ctagcgatat ctttagcaag tgtcatggta
     3541 ttctcctttt atttttctaa tctagttctt taattccagt cctgtgtcca ttcatccttg
     3601 atggcatgat aagctgcaac aggatcctca gcttgggtaa tgggacgtcc tactacgata
     3661 tagtcactgc cgatttgata ggcatcagca ggtgtcatga ctcgtttttg atctccaact
     3721 gcaacaccag ctggacgaat ccctggtgtc agacagataa aatctggatt ggtagcctgc
     3781 ttgatgactt gtacttcctg agccgagcaa acaacaccat ccaagccagc ttcagctgtc
     3841 ttcttggcat agtgaatcac agactcttgc agactggttt ggatattttg aaactcctgc
     3901 atctgagctt ctgacgttga tgtgagctga gttacagcga tcaatttagc ttgacttcca
     3961 agaccttcac gcgcagcctt catcatctct acaccaccag ccgcatgaac attggtcata
     4021 tccacaccaa gctgagatag gatcttcatg gctgacttga ctgtattagg aatgtcatga
     4081 agtttgagat ccaaaaagac actatgaccc aaacctttta agtaggacac aatctcaggc
     4141 cccgctgcgt aataaagctc catccctacc ttgagataaa ggctttcttc tgctgggaaa
     4201 agagctaaaa attccttgac cgcctcaaaa ctaggaaaat caagagcaat gattgaacga
     4261 tgttctcgca taggtttctc cttttacctt tactagtgag agagtctggc caacagaagc
     4321 cacgaaaaaa ggaacctgcc aacagcaagg ttccacgaaa aatgggtagt ctccaccctt
     4381 tcaccgtcta accttagctg cctctctgga ctgctttaaa ggttttctat tattctatta
     4441 tttctactag cacttgtcaa gtaaaaacat ggtcactaaa gaactataag agaaaaggta
     4501 aacctagcga cgcgatgaac gctgggtcgt ttggtttcga ttgctctctt cctcttgttt
     4561 tttctgttct tcttcttgtt ttttctcagc ttccttggcc tcttgtttgg ctttttcctc
     4621 agcttccata attaatttat ccgccacagt gtaactgtaa attccagctt ccatgtcaat
     4681 cacactcggt tctgacaatt gtggcttaat cttactgtaa tatggcagtt tcttactcat
     4741 ttcagataga ggaaccaaga cttcgtccga atcattcatg gtcaatcgaa ttaaatcgga
     4801 tgtcaccttg cttggggcta attccacctt ttggatagcc gccttgagtt ctgggctaat
     4861 ttgagcaagt tctgagacaa aaaccttgat ttgttcacta tcattaaaga gaactgataa
     4921 ataagtttct ggtaaactgt tcagactcac agaactagtc tcaagctgac cactggaaag
     4981 aataggataa tgattttcac cagaaatata gtaggccaca atatcatatt ccttgacctt
     5041 aatagtgaac ttagttggaa attgatagac aagttgagct gattcaaccc aatagttaga
     5101 cttaatctgc ttttcatatt ttgccttgtc tagcagaagg ttaatcgtat aatccgaatc
     5161 ctgaatgcct gaagcctgtc gaatatcatc agctgtagtt tgcaccgttc cctcaacacg
     5221 aatatctttc atggtcgcat aaggactgag caagtaggca gagacaatca ataaaagcag
     5281 acttggaaat aaaatcgtga aggctcgcaa gatatggata ccaggaatct ttgctttggc
     5341 tggtttttcc tttatagcct ttttagcaag ctttttatcc tgttcctcct tctctttaga
     5401 ctctgattct tctatctctt ctttttcttt gtcagcctct gaggatgcca ctttttcttc
     5461 agactcttcc ttagctgatt ctgaatcttc ctggtctgtt tcactctcct ggtcctgttt
     5521 atcctctgac ttctcagatt cttctcccat tcgagcttgt ctttcctttt ccttctcctc
     5581 agctagagcc gcctcttctt cagccttctt ttttagatat tcttggtttc gtttctgcca
     5641 ttctgataac tctttcaatt cttcgagggt ttctttgtcc tcatttttct tatcttttga
     5701 catttacttt ccttatgata aatctttttt caacaattga taaaaatctg ctagagattt
     5761 caattcctta gaagctttca tcttagcttg gtaatcttcc ttgtgactta gtaagtgaga
     5821 aagcttctct tccaaactat ccaaggtcaa atcgctttct tgaaggtctt ctgcatagcc
     5881 tttttttaca aagtaagctg cattttcaat ctggtcacca cgactagctt cacgaccaag
     5941 cggcacaatg acatgcaatt ttgctatcgc caagagctca aaaatcgtat tggcaccacc
     6001 tcgtgtcaca acaatatcag ccaattccat caagggttga tagagatcgg tcacatagtc
     6061 aacacgaaaa agattttgcc tcaactcatt cagactagaa tctccagtta gattgataat
     6121 attgtagcgc tctgttagtt ctttcttatg gtctgtcacc aattggttaa agacacgagc
     6181 gcctgcagaa ccgccgacaa acaatacagt tggcaatttg ggattaaagt gggtttgaat
     6241 atccaccaat tcatctggtt ctggagtgtt tttgtccgaa accttggtca ccgctcccac
     6301 atgctcgacc ttagccaaac tcgaagcttg ttcaaaggtt gaatacatct tagtcgcaaa
     6361 tttataggcg attttattgg ccaagcccat agacaggtca gattcgtgaa taaagacagg
     6421 cactcctgac acacgcgcag cgataacagg cggtactgag acaaagcccc cctttgaaaa
     6481 aagggtctgt ggacgcagtc gcaacatgat aaagagcgat tggacgattc cccagccaac
     6541 tttgaagacg tccagcatat tttgccaaga gaaatagcga cgcaatttcc cagtcgcaat
     6601 agaatggaag gtgacatcca aacctgactt aaggatttct tggtgttcga taccacgctt
     6661 gtccccgata tagtggactt cccaaccatc ttcgatgaac ttgggcatta acaaaagatt
     6721 gagggtaacg tgtccaaccg tccccccacc tgtaaagaca atttttttca tattttattc
     6781 ttttaactcc gctactgtgt cgataaagag gtcgccacgt acttcaaagt tagcatacat
     6841 atcccagctg gcattggcag gactaagaag aaccacatct ccttgagtcg caagttcata
     6901 ggccttacgg gtcgcatctg caatatctgt cgcctccaca taagcgacac cagccttgtc
     6961 tgctgcccgt ttgacacgtt ctgcagattg acccaggatg accatcttct tgagtccagt
     7021 aatgtctggc accaattcgt caaactcatt gccacggtcc aaaccacctg caatcaagac
     7081 gaccttgctg ttgtcaaatc ctgataaggc tttttgagta gccaagatat tagttgattt
     7141 actgtcgtta tagaatttaa cacccttgat gtcatccaca aactggagac ggtgtttgac
     7201 accaccgaag gctgaaagag tttccttgat ggtttgattg tccacatcac gaagcttggc
     7261 tacagcaata gtcgcaaggg cattttccac attgtggcta cctggaacac cgatttcatt
     7321 cgctgccatg actacttcac cacggaagta gagttgacca tcttccagat aagctccatc
     7381 aaccttttca agtgttgaaa atggtacaac agtggcttct gtcttggaag tcaagtcttt
     7441 tgccaagtct tgattaaagt tcaagacaag gaaatcagct gctgtcatct tgttctggat
     7501 attccacttg gctgctacat attccgaaaa tgacccatgg tagtcgatat gagttggcat
     7561 gaggttggta ataaccgcaa tctctggatg gaattcttga acacccatga gttggaaaga
     7621 agaaagttcc ataacaagcg tgtccttatc tgatgctatt tgagcaacct gactagctgg
     7681 atagccgata ttccctgata aaagaccatg ttgcccagca gcagtcaaaa cttccccaat
     7741 catagtcgtt gtggttgtct taccgttcga tcctgtgata ccaataatcg gtgcttctga
     7801 aatcaaataa gccaattcca cctcagtcaa gactggaatt cccttggcca aagccttttc
     7861 aatcatggga ttgttgtagg ggatacctgg atttttcacc ataagggcaa actcttcatc
     7921 caagagttcc aaaggatggc cacctgtaat gaccttgatc ccttcttcca gcaaactttg
     7981 ggcagctgga ttgtcctcga aaggtttccc atcatttact gtcacaatgg cacctagctt
     8041 gtccaacaaa cgagctgcag attcaccaga cttggccaag cctaaaacaa ggactttctt
     8101 atttttaaat tgatctatta ctttcatgtc tcgaactcca tttctactcc tactatttta
     8161 ccatttttat ggaaataaaa aagccacaaa gtgtgtttgt gactctttct tctaactgaa
     8221 tcttaccata tcatctatgt gataaatcgg taactcgaat gacctgatcc acttgctccc
     8281 aaatcagagg attatgggtc gcaataataa tggtccgatt cggatttttt aaagattcta
     8341 ggatggaaag taattcctca gagtttttgg ggtctaagga agcggttggt tcatctgcga
     8401 ggatcaaagg tggatccttt aaaattatct tcgctagtgc aacacgttgt gcttctcctc
     8461 ctgataactc aaatataggt tgcttcaaat ccaaataaga gaggtttaca cggtttagag
     8521 cttgtttcat caaagagatt ttctcttttt ccttcaactt tttaccaact aaacccagat
     8581 tgagattctc tttgacggtt tggctttcaa ttaagccaaa atcttgaaat aagtatccta
     8641 agtaatctct aaagaaaaca gaaggcttaa tgtccttaag agaagtgcca tcatatatga
     8701 tttgcccttt gtcatatggc tctaatcgtc caatcatatt caagagtgtt gtcttaccac
     8761 agccacttgt accgattaag gcataaattt tcccaccttc aaaatgaaga ttcgtatctg
     8821 aaaatagctg acggcttcca aattttttag atatattctt tagttcaatc atcctatttt
     8881 cctttcataa ttgtcataga aacacgagat tctttctgcg cttgacggta aagcgtcaaa
     8941 actgcactaa ctagaaagac caataaagtg agcaagccaa tcaccaagtc tcgactgctt
     9001 aaaataaaga gactagcacc aaatacaaaa ctggcaaatt ggctaaccat atactgagca
     9061 tgtgtttcaa aaaatcgtaa acctgaaatt cgtttaatca agatatctcg gcggaattgc
     9121 tcgaaatata gaagattgac agaataaaag agtaacaagg aactggctat tccaacaata
     9181 gctcctaaga ttaaagttgc tgtttcagtt tgaacttcat tataacgagt tagataaaca
     9241 cttcttcctt ctttaagata ggatacttgc tcataaattc cagctttctt caagagttct
     9301 agcccactct catatccttt gataaagagt tgttttccag cattgataga ccaactagat
     9361 aaggatataa aactatcacc tgtagaagtc ggcgtgaata caactaaaat cggatcagtc
     9421 aaatactgag tagatacggg attctcaccg ttattataaa caaaccgctt ttctcccatt
     9481 gaaagataac taacgtgcgc tttcatctca taatccaaag gagcacttgc ctcctcacca
     9541 gattttccat aataactcaa tctttcttca aaaactttct taagttctgc ttctcgagag
     9601 cgcaaatgtt ctgggagcaa gaggataaac tcaccttttt ggagatgggc taacttctgt
     9661 ttggtctcag catctaccac gaccttttcc ttgtccaaat aactgggact aacatagagc
     9721 gtattagcat ctggactata ggtatccagt gtctctccct gttcattttt tccttgtgga
     9781 ttggcaaaat ggagcagatt atcctttaca taaagagctt gttcttcttc gattgcttcc
     9841 ttggcaaagg cataccactt gctctgattt tctgtatctt ttcctctatc acctaagcca
     9901 aaggaaatct ggtaatagtc tgctctgtcc tgccatgctt gttttgaaat ttcaagttct
     9961 ttcaatcgtt ggtaagacgt caaacctgtc ttaacagcgt agcctactgt aaaaacagct
    10021 actaactgac acaatagggt taaagccatc aagcgtttaa ggggtaatct tcccttaata
    10081 acgggaacta atgctttgta actcaaactc attaggtaaa ggagcattag taaaattgaa
    10141 atcgccaata aaaacaacag atagaaacta atcccaaaac cataggtggc taacaagata
    10201 ggataaaaca aaccttgact aaaaagaacg actcccccac ctaggaagga aaggagggct
    10261 gatagaagga gccacttgat atcagtagat aaaccccaat tattcacccc taatctaaaa
    10321 accatccaga atccttgcct tagcttagat cctggatggt ttcttttttc acccaatggg
    10381 tgttttttac tagacaaaaa agagtttccc ctttatggta taagtgtaga aaaaaacaca
    10441 aaaagaaagg aaactcatat gaacagttta ccaaatcatc acttccaaaa caagtctttt
    10501 taccaactat ctttcgatgg aggtcattta acccagtatg gtggtcttat cttttttcag
    10561 gaactttttt cccagttgaa actaaaagag cggatttcta agtatttagt aacgaatgac
    10621 caacgccgct actgtcgtta ttcggattca gatatccttg tccagttcct ctttcaactg
    10681 ttaacaggct atggaacgga ctatgcttgt aaagaattgt cagctgatgt ctactttcca
    10741 aaattgttgg aaggagggca gcttgcttca cagccaacct tatcccgttt tctttccaga
    10801 actgacgagg aaacagtcca tagtttgcga tgcctcaacc ttgaattggt cgaattcttt
    10861 ttacagtttc accagctaaa ccaactcatt gtagatatcg attctaccca tttcacaact
    10921 tatggcaagc aagaaggtgt tgcttataac gcccactatc gtgctcatgg ctatcatcct
    10981 ctttatgctt tcgaggggaa gacaggttat tgtttcaatg cccagcttcg tcctggtaat
    11041 cgttattgtt ctgaagaggc agacagcttt atcacacctg ttttagaacg gtttaatcaa
    11101 cttctctttc gaatggatag tggctttgcg accccaaaat tatacgattt aattgaaaaa
    11161 acagggcaat actacctcat aaaactcaag aaaaatactg ttctgagccg tcttggagac
    11221 ctttccctcc cttgcccaca ggatgagaac ttaaccatct tgccccactc cgcctactca
    11281 gaaactctct atcaagcagg atcttggtcg cacaagcgtc gtgtctgcca gttctctgaa
    11341 cgaaaagaag gaaacttgtt ctacgatgtt atttctctcg ttacaaatat gacgagtgga
    11401 acaagccaag accagtttca gctttatcgt ggacgtggtc aagccgagaa tttcatcaag
    11461 gagatgaagg agggattttt tggcgataaa acggatagtt caaccttaat caaaaacgaa
    11521 gttcgtatga tgatgagctg tatcgcctac aatctctatc tttttttcaa acatctagct
    11581 ggaggtgact tccaaacttt aacaatcaaa cgcttccgcc atctttttct tcacgtggtg
    11641 ggaaaatgtg ttcgaacagg acgcaagcag ctcctcaaat tgtctagtct ctatgcctat
    11701 tccgaattgt tttcagcact ttattctagg attagaaaag tcaacctgaa tcttcctgtt
    11761 ccttatgaac cacctggaag aaaagcgtcg ttaatgatgc attaaagaac agtcgagatg
    11821 aaaaaatcgt gtgacgcacc aagggaggag tctgcccttt tgaggaaatc tagcgaggaa
    11881 aaacgatact ggaacagcag aaagtaaaac tgacctcatg aggaggaaga aagtggctca
    11941 tgaggtcagg ggttttgtaa gttacatcta gttgagagag gtatgaatga tttgggataa
    12001 agaatccccc atgatggata agagagtctg accagaaaag agttttatac ctgctgctct
    12061 catttcctta atccgagtga taatcactaa agcaaagaaa gataagccaa atattgctaa
    12121 actaattaaa ataaggggat ttagtaatat tcgaaaagca agaaaatagg gcggtatctt
    12181 tcggtcagca cttgctttat aacccaaatc tcctaattta tcggcaagct tttctttcgt
    12241 caaggagcct gacaaaagga gataactatt tagcggatca ctacgttcac gactttcttg
    12301 gctagcttct tggaattctt ttggtaaagt tccctgacca taagttgcat aagtaaagtg
    12361 agtcgtccca tccttactcg gctctacaat tcttctagct attaaactct gttctgagtt
    12421 tgcaaaattc tccaattcct gttcaaatac ctcacgcgtc ggttcctgag tatctttttt
    12481 gacacgaagt aaagaaacgg aatcatagct tgcatataaa tattgtggcg cacgtaagac
    12541 aataatccaa gcaaggaaga agctgagaaa aaaagttgat aataatatga atagtttctt
    12601 catagtagac tccttgtaaa caaaattccc cctgtaattt cttacaaggg gaacgattta
    12661 aatcaatgaa cgattagtca taatcacagt aaaatgctac ttgttctccc catttagtcc
    12721 aaatccatgc ataggaggtt tttccagcag gagcttctcc tttatcagaa tgaccatccc
    12781 atctgctcac gatggatgaa taatgatatt ttttaccatg atagtaattt gaaaaagcct
    12841 aaccacctcc tgaaccttct ccatatgtcc atactcctcc atctggatat tatacagcag
    12901 ctgatgcagc tcccaataat gtaaaacttg aaataagagc tagagcaagt aatctatgtt
    12961 ttttcgtttt cattttattt tttctttcaa aaaaatcaca ccttgagcaa caatgcaaca
    13021 aaataaatcc tcctctctct tttattgaaa ccgctttctt gtgtgataag aataacattt
    13081 ttattatttg ttgtcaagga aaaaatcgaa tttttt
//
LOCUS       DAVKGC010000051        13113 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKGC010000051 DAVKGC010000000
VERSION     DAVKGC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.051"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            59..1318
                     /locus_tag="VW049_001227"
                     /pseudo
     CDS             59..1318
                     /locus_tag="VW049_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            1414..2688
                     /locus_tag="VW049_001228"
     CDS             1414..2688
                     /locus_tag="VW049_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9432354.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRQEKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            2698..3576
                     /gene="truB"
                     /locus_tag="VW049_001229"
     CDS             2698..3576
                     /gene="truB"
                     /locus_tag="VW049_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9432355.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLYEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALSLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            3817..4119
                     /locus_tag="VW049_001230"
     CDS             3817..4119
                     /locus_tag="VW049_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9432356.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            4227..4865
                     /gene="udk"
                     /locus_tag="VW049_001231"
     CDS             4227..4865
                     /gene="udk"
                     /locus_tag="VW049_001231"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9432357.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            4993..6333
                     /locus_tag="VW049_001232"
     CDS             4993..6333
                     /locus_tag="VW049_001232"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9432358.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEDLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            6311..6523
                     /locus_tag="VW049_001233"
     CDS             6311..6523
                     /locus_tag="VW049_001233"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9432359.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            6520..7395
                     /locus_tag="VW049_001234"
     CDS             6520..7395
                     /locus_tag="VW049_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9432360.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQIPFE
                     TESIVSVVESLRING"
     gene            7388..8203
                     /locus_tag="VW049_001235"
     CDS             7388..8203
                     /locus_tag="VW049_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9432361.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEV
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            8196..8627
                     /locus_tag="VW049_001236"
     CDS             8196..8627
                     /locus_tag="VW049_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9432362.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            8634..10301
                     /gene="recN"
                     /locus_tag="VW049_001237"
     CDS             8634..10301
                     /gene="recN"
                     /locus_tag="VW049_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9432363.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDKQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSYLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            10303..11031
                     /locus_tag="VW049_001238"
     CDS             10303..11031
                     /locus_tag="VW049_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9432364.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            11110..12933
                     /gene="lepA"
                     /locus_tag="VW049_001239"
     CDS             11110..12933
                     /gene="lepA"
                     /locus_tag="VW049_001239"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9432365.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         3900 a         2413 c         3150 g         3650 t
ORIGIN      
        1 gtaaactata actcaaggtg ctacaatatc cttaataaaa tgatatggag gtcaccttat
       61 gacttgtgat tttaaatctg aaactctaca actacatgct ggtcaagttg tggctccagc
      121 tactaagtct cgtgcagtgc cgatttatca aacaacattt tttgtttttg atgacacgta
      181 ggaaggtgcc gatctgtttg ccttgaggaa accagggaac atttatactc gtatcaccaa
      241 tcctacaaca gctgcccttg aaggtggtgt tgaagcgcta gcaacagcat caggtatgac
      301 tgcagtgact tatacgattt tggcgcttgc ccatgctggt gaccatgtag tggctgcttc
      361 gactatttac ggtggaacct tcaatctttt gaaagaaccc cttcctcgtt atggtatcac
      421 aacaaccttt gtcgatattg ataatttgga ggaagtagaa gcagctatca aagacaatac
      481 caagcttgtc ttgattgaaa ccttgggtaa ccccttgatt aatattccag acctggaaaa
      541 actggcagag attgctcata aacatcaaat cccacttgtg tcagacaata cttttgcaac
      601 accttatttg attaacgtct tctctcatgg cgttgacatt gccattcact ctgcgactaa
      661 gtttatcggt gggcatggta caactattgg agaaataatt gtcgatagtg gtcgttttga
      721 ctggacggct tcagggaaat tccctcaatt tgttgacgag ggtccaagct gccacaattt
      781 gagttatact cgtgatgtgg gtgcagcagc ctttattata gctgttcgag ttcaattgct
      841 tcgtgataca ggtgcagcct tgtcaccatt caatgctttc ctcttgctac aaagacttga
      901 aaccctttca cttcgtgtgg aacgccatgt acaaaatgct gagacaattg ttgattttct
      961 tgtcaaccat cctaaggtag aaaaggtaaa ttatccaaaa cttgcagata gtccttatta
     1021 tgccttggct gagaaatact tgccaaaagg tgtcggttca atctttacct tccacgtcaa
     1081 aggtgacgag gaagaagcac gcaaggtcat tgataattta gaaatctttt ctgaccttgc
     1141 aaacgcggca gatgctaaat cgcttgttgt ccatccagca acaatcactc acggtcaatt
     1201 gtcagaaaaa gacctagaag cagcaggtgt cacaccaaat caaatccact tgtctatcgg
     1261 tcttgaaaat gtagaagatt tgattgaaga cttacgcttg gccttggaaa aaatttaaag
     1321 taaaagaaga taaacagtgg gttttgactc attgtttttg attttccctc aggcatgata
     1381 taatggttac agaagtctag aaagaggaat gtaatgaacg aaatcaaatg tcccaactgt
     1441 ggggaagtct ttacagtaaa tgaaagccag tatgctgaac ttttgtccca agtgagaacg
     1501 gcagagtttg ataaggaact acacgatagg atgaggcagg aactggcctt ggctgagcaa
     1561 aaggctatga atgagcaaca gactaaactg gctcagaagg atcaagaaat tgcgcaattg
     1621 cagagtcaaa tccaaaactt tgatacagaa aaagaattgg ccaagaaaga agttgaacag
     1681 acaagccatc aggccttatt ggctaaggac aaggaagtac aggccttgga aaaccagttg
     1741 gcgaccttgc gtttggagca tgaaaatcaa ttgcaaaaga ccctttctga cctagaaaaa
     1801 gaacgcaatc aggtcaaaaa tcagctccta ctgcaagaaa aggaaaatga gttgtctttg
     1861 gcttctgtta agcaaaacta cgaagcccag ctcaaagcag ccagtgaaca agtcgagttt
     1921 tataagaatt ttaaggctca acaatctaca aaagcgattg gggaaagcct agaacagtat
     1981 gcagagagtg agtttaacaa ggttcgtagt ttcgcctttc caaatgctta ctttgagaag
     2041 gataacaagg tctcttcgcg tgggtctaaa ggggacttta tcttccgtga gtgtgatgaa
     2101 aatggagttg aaatcatttc tatcatgttt gagatgaaaa acgaagcgga cggaacagag
     2161 aagaagcaca agaatgcaga tttttacaag gaattggaca aggaccgtca ggagaagaac
     2221 tgtgagtatg ccgttttggt gaccatgctt gaggccgata atgactactt taacacaggg
     2281 attgttgacg tcagccacga gtatgaaaaa atgtatgttg ttcgtcctca attctttatc
     2341 cagttgattg gtctcttacg taatgcggcg ctaaattccc taaaatacaa gcaggagttg
     2401 gccttggttc gcgagcaaaa tattgacatt acgcattttg aggaagattt ggatgccttt
     2461 aagctagctt ttgctaagaa ctataattca gcttcgacta actttggaaa agctattgat
     2521 gaaatcgaca aggccatcaa acgcatggaa gaggttaaga aattcctgac tacatctgaa
     2581 aaccaactcc gtctagctaa caacaaattg gaagatgtct ctgttaaaaa attgacccgg
     2641 aaaaatccaa caatgaaagc gaagttcgaa gcactgaagg gggagtagaa agcaaaaatg
     2701 aacggtatta tcaacttaaa aaaagaagca ggaatgacct cgcatgatgc ggtttttaaa
     2761 ctgcgtaaga ttttgggaac caagaaaatt ggtcatggtg gaaccttgga tccggatgtg
     2821 gtgggtgttt tgccgattgc ggttggcaag gcgacacgca tggtcgagtt tatgcaggac
     2881 gagggtaaga tctatgaggg ggaaattact ctgggttatt ccacgacgac tgaggatgct
     2941 agtggggaag tgatcgcaga aacccctgtt ttgtctccct tgtatgaaaa gcttgttgat
     3001 gaagcgattg ctagcttgac tgggcctatt acccagattc cccctatgta ttcggcagtt
     3061 aaggttaatg gtcgcaagct ctatgagtat gcgcgtgctg gtcaggaagt ggagcgtcca
     3121 gaacgtcagg tgatcattta tcaatttgag cgaacaagtc cgatttctta tgatggccaa
     3181 cttgcccgat tcacttttcg tgtaaaatgc agtaaaggga cttacatccg tactttgtca
     3241 gttgatttgg gtgaaaagct tggttatgcg gctcatatgt cccatttgac tcgtactagt
     3301 gctgctggct tacaattaga agacgctctt tccttggagg aaattgctga aaaagtagag
     3361 gctgggcaat tagattttct ccatccttta gagattggga caggtgacct tgtcaaagtt
     3421 ttcctaactc cagaagaggc tacagaagtt cgctttggtc gttttattga gctagaccaa
     3481 acggacaaag aactggctgc ctttgaagat gataaattgt tagccattct agaaaaacgg
     3541 ggcaatctct ataagccaag gaaggttttt agctagatcg tttaggaata aaaatcgggt
     3601 gatagataac aattgcttga taaaacccca tactaatagt agaatggttt tgggaattat
     3661 aatattccaa ttgttgcgag ttgtaggtac tcaaataatc tatatagaaa tttagaggtg
     3721 tgaaatgaag caatttaaaa ttctttcaga taaatattta gagtccatta caggttctgg
     3781 tgggaactta ggcccaggat ttggtgtgat aattccatga tgcgaaatga gtttcgagaa
     3841 agggtggagc aacttcttca acaaaaagaa ataaatgaaa atagtgagtt gagtcacctg
     3901 tttcgtcttg ctatacaaaa tttagacaga aatgaaaaat accaatcggt catggccaat
     3961 ttgagtcaag ggttgtcact ttacctcatg acgcatcatt accaggcacc taagtctgtc
     4021 attgattttg gtttatggat tgccaaagct cctagccagg aaagagggag actggctttc
     4081 ttgcagatgc ttgctcaaac cctacagggc tttcgttaaa actgtcgaac atcagaggga
     4141 agactctggt gttttttgct gatgagacta taaattttgt aaaaaatgtg atagaataga
     4201 cgacggataa aataacggag gatagcatgc aaaatagacc aatcattatt ggagtgacag
     4261 gtggttctgg tggtggtaaa accagtgttt caagagccat tttatcgcat tttcctgatg
     4321 aaaagatttc catgattgag catgattcat actacaagga tcagtctcat ttgacctttg
     4381 aagagcgtgt caaaaccaac tatgaccatc cttttgcctt tgatacagac ttgatgatcg
     4441 agcagattaa ggaattgttg gcagggcgtc cggtggacat cccgacctac gactatacag
     4501 agcatacacg gagtagcaag acctatcgtc aggagcctca agatgtcttt atcgttgagg
     4561 gcattttggt cttggaggac aagcgtctgc gcgatttgat ggatatcaag atttttgtgg
     4621 atacggatga cgatgtgcgc attattcgtc gtatcaagcg tgatatggag gagcgtggcc
     4681 gtagccttga tagcgttatt aaccagtact taggtgtggt caaaccaatg taccaccagt
     4741 ttatcgagtc aactaagcgt tatgctgata tcgtcattcc tgaaggggtt agcaataccg
     4801 tggctatcga cctgttgacg accaagattg caaagatttt ggaagaagct cgaaacagca
     4861 aataatcaca tgtggaggcc ttgcctcctt tttctatttt tccctcataa tggtacataa
     4921 atggagattt ttaggcatat ttttggtata ataataccca tgaaagagca agtaaatgaa
     4981 tagtaggtgg agatggaaaa gtatttatcg gtaacaactt tgaccaagta tctgaaaatg
     5041 aaattcgata aagacccata cttggaacgg gtctatttaa ctggtcaagt ttccaacttt
     5101 cgtaaacgac ctactcacca atatttctcc ctaaaggatg accatgcagt tattcaagcg
     5161 accatctggt ctgggattta tcagaaatta gggtttgacc tggaagaagg aatgaagatc
     5221 aatgtgattg ggcgtgtaca ggtctatgaa ccaagtggta gctactccat catcattgaa
     5281 aaagctgagc ctgatggggt tggggcgctt gcgattcagt ttgaacaact taagaaaaaa
     5341 ttgacagaag aagacctgtt tcaagaacgc ttcaagcaag ctctgcccca attttctaag
     5401 agaattggtg tagtaaccag ccgtagtgga gccgttattc gagatattat cacgaccgtc
     5461 agcaggcgat tcccaggtgt tgacatcctt ctttatccga ccaaggttca aggtgaaggg
     5521 gctgcggagg aaattgctag aaatattgcg cgtgctaatc aacgggacga tttggatttg
     5581 ctcattattg gtcgtggagg tggttctatt gaggatctct gggcctttaa cgaagaaatt
     5641 gtggtacgag ctatttttga atctcgttta ccagttatct ctagtgtggg gcatgagacg
     5701 gatgtgacct tggcagattt tgtggcagat cgacgcgctg caacgccaac agcggcggct
     5761 gaactggcaa cacctgtgac caagttggat gtattagctc atttgcaaaa tcaggaaaaa
     5821 cggatggcaa cggcagtccg aaatgttcta tctaagaaac aagaggcttt gaaaaaatgc
     5881 agtcagtctg ttatctttag acaacctgag cgcttgtatg acggttattt gcaacgcttg
     5941 gaccaactgc aactgcgttt gaaacaaagt ttgcgaactc ggatttctga taacaaacaa
     6001 ttagttcaag caagaactca tcaattagta caattatcac ctgttaccaa aatccaacgc
     6061 tatcaagacc gtttaggaca gttggacaag ctcttaggta gccaaatggc gttagtttat
     6121 gacgccaagg ttgctgaggc caagcgactt tcggaagctt tgctcatgtt ggatactagc
     6181 cgaatcgtgg cgcgtggtta tgctattgtc aaaaaagaag agtccgttgt agattcggtt
     6241 gagagtttga agaaaaaaga ccaagtaacg cttttgatgc gagatggtca agtagaatta
     6301 gaggttaaag atgtcaaaac aaaagaaatt tgaggaaaat ctagcagaac tggaaaccat
     6361 tgtccaaagt ttggaaaatg gtgaaattgc tctggaagat gcgattactg cctttcaaaa
     6421 gggcatggtc ttgtcaaaag agctccaagc tacgctggac aaggctgaaa agaccttggt
     6481 caaggtcatg caagaagacg gaacagaaag tgattttgaa tgaaaaagca agaaaaatta
     6541 gctcttgtcg agtcggcttt ggaagatttt tatggagacc agcagtttgc ctctagttta
     6601 cgggagtctg ttctctattc tattcatgct ggtggcaagc gtattcggcc ttttctcttg
     6661 ttagaagttc tggaagcctt gcaggttgcc atcaaacctg ctcacgcgca ggtagctact
     6721 gccttggaga tgattcatac agggagcttg attcacgatg accttcctgc tatggatgat
     6781 gacgattatc gaagagggcg gttaaccaat cacaagaaat tcggtgaagc tatggccatt
     6841 ttggctggag atgccttatt cttagaccca tatgccttga ttgcgcaggc agatttgcca
     6901 agtcagatca aggtggactt gattgccaac ttatcccttg cttcaggtag tctgggtatg
     6961 gtggcagggc aagttttgga tatggagggc gaacaccagc acttgtctct ggaagaactt
     7021 cagactattc atgccaataa gactgggaag ttactagcct atcccttcca agcggcagct
     7081 attatagctg aattgtcacc tgaaatgcag gtgaagctga aaactgtggg tgaattgatt
     7141 ggacttgctt ttcaagtcag agatgatgta ctggatgtga cagctagttt tgaggaaatc
     7201 ggcaagacac ctcaaaagga tctgcaggca gaaaaatcaa cctatcctgc cttgttgggc
     7261 ttggaagagt ccattgcctt ttgtaaccaa accctggatc aagctaatga aaaattagaa
     7321 gaaattgccc agcagattcc ctttgaaaca gaatcgattg taagtgtagt agaaagtttg
     7381 agaatcaatg gctaaggaaa gagtggatgt actagcttat aaacaggggt tgtttgaaac
     7441 gagagagcag gccaagcgag gtgtgatggc tggcctagtc gtagcagtcc ttaatggaga
     7501 acggtttgac aagccaggag agaaaattcc agatgacacc gaattaaaac tcaaggggga
     7561 gaaactcaag tatgtcagcc gtggtggttt gaaactggaa aaggccttgc aggtctttga
     7621 tttgtcggtg gatggcgcga ctacgattga tatcggggcc tctactggag gttttaccga
     7681 tgtcatgcta cagaatagtg ccaagttggt ctttgcagtc gatgttggta ccaatcagtt
     7741 ggcttggaaa ttacgccaag acccacgagt tgtcagcatg gagcagttca atttccgcta
     7801 tgctgaaaag actgatttcg agcaggagcc gagctttgcc agtattgatg tgagtttcat
     7861 ttcccttagt ctgattttgc cagccttgca ccgtgtcttg gctgatcaag gtcaggtggt
     7921 agcacttgtc aaacctcagt ttgaggcagg acgtgagcag attgggaaaa atggaattat
     7981 tcgagatgct aaggttcatc agaatgtcct tgaatctgta acagctatgg cagtagaggt
     8041 aggtttttca gtccttggct tggacttttc tcccatccaa ggtggacatg gaaatattga
     8101 atttttagcg tatttgaaaa aagaaaagtc agcaagcaat cagattcttg ctgagattaa
     8161 agaagcagta gagagggcgc atagtcaatt taaaaatgaa taaaaaagag agacttgaaa
     8221 aaattagaag attggtgaca gattatcaaa tcggcacgca agaagaaatt gtagaacatt
     8281 tgaaagaagc aggtatcact gccactcagg cgacggtatc ccgagatatc aaagagttag
     8341 gtattgtcaa aattcctttg agagacaaca cctatgtcta cgaattacca aaatcaatcg
     8401 taaaaagtct gcaactggct gaagacaata tcgaatcggc tgaattgatg gataagatga
     8461 tcaatctcca agttattcca ggaaatacgg cttttgtaaa agctcagtta atcgagactt
     8521 ttgcagacaa gatttttagc tgtttgactg atgatagctc gattttagtc attgccagaa
     8581 gtggaagtct agcagaggaa atctttgaac aagtaaaaaa ttggtaggtc tatatgttac
     8641 ttgaaatttc gataaaaaac tttgccatta ttgaggctat ttccctcaat tttgaaaagg
     8701 ggatgactgt cctgactggt gaaacgggtg cagggaagtc gattatcatt gatgccatga
     8761 atatgatgtt gggagctcgt gcgacgacag atgttattcg tcatggtgca ccaaaggcag
     8821 agattgaggg gcttttctca gttgagaata gtcgtctttt acaggaaatt tttgataagc
     8881 aaggtttaga attaggtgat gaaattatca tccgtcggga aatcttgcaa aatggtcgta
     8941 gtatcagccg tgtaaatggt cagatggtta atctgtctgt tttgcgagct attgggcaac
     9001 atcttgtaga tattcatggt cagcatgacc atgaggagtt aatgcgtccc caactgcata
     9061 tccagatgtt ggatgaattt ggtgatgccg ctttttggga cttgaaagaa acctatcaaa
     9121 cgagttttga tgcctatcgg aaaatgcgca agcaggttct ggaagtcaag aaaaaccaac
     9181 aggaacacaa ggcacgtatc gaaatgttgg aatttcaaat ggcagagatt gaggcagcaa
     9241 acttgcaggc tggagaagac ttggctctca atcaagagcg agataaactc ctcaaccaca
     9301 aaaatattgc ggatacactg accaatgcct acagtatgtt ggacaatgaa gatttttcaa
     9361 gtctggccaa tgttcgttca gctatgaatg acatggaaag tgtcgaagag tatgatcctg
     9421 aataccgtga aatttcaagc tatctgtctg agacctacta tgttttagaa gacattagca
     9481 aacgtttgga agctatcatt gaggaccttg attttgatgg caatcgcctg atgcaggttg
     9541 agaatcgttt ggacctcctt catactatta ctcgtaagta tggtgggact gttgatgatg
     9601 ttttgcttta ttttgccaag attacggaag aatacaatct cttgacaggc aataatcttt
     9661 cgtctgagga catggaagca gagcttaaga agttggaagt caatcttgtc aatttggcag
     9721 gtcaacttgc ttctgctcgt cataatttgg ctcagcaact cgaagctgag attaaacaag
     9781 aactgcaaga tctttatatg gaaaaagccc agtttcaggt tcgttttagt aagggaaaat
     9841 tcagtcgtga gggaaataaa atggttgagt tttatatttc aaccaaccct ggagaagact
     9901 ttaaaccctt ggttaaggtt gcttctggag gggaattatc tcgtctcatg ttagccatta
     9961 agtctgcctt ttcacgtaaa gaaggcaaga ctagcattgt ctttgatgag gtggatacgg
    10021 gagtttcagg tcgtgttgct caagctattg ctcagaaaat tcataaaatt ggtcagcatg
    10081 gtcaggtttt ggctatctcc catttgccac aagtaattgc gattgcagat tatcaattct
    10141 ttattgagaa gattagtaat gaccattcaa cggtttcgac tgttcgtctc ttgacggtcg
    10201 aagagcgagt ggaggaagtt gccaagatgt tggcaggtga tgatgtgaca gaagcagccc
    10261 tgacgcaagc aagagaattg ttgagaaaca gggagaaata agatgacaga ttattatgta
    10321 attggagatg ttcacggaaa agctgggatg ctggaagacc ttctcaaaac ctgggatggt
    10381 cagccccagt tgctctttct aggtgatttg attgacagag gtgaggatag tcaccgtgtt
    10441 cttgaaatgg tcaaggactt ggtggacaat caaggggcta tctgtttgtc cggaaaccac
    10501 gagtatatgt ttttgacttg gctagatgat ccagaagaaa gttatgacca ttatagtcgc
    10561 aatggtggag atacaaccat taactctatc ctaggtcgtc ccttggatgc accagttgat
    10621 ggagtagaag atgccaagcg ggttgccgct gaagcgtcag acttggtcga attcattcgt
    10681 caaatgccat ttgtagtaga gacagacaag tatatatttg ttcacgcagg tattgatttg
    10741 actttggacg actggcatga aacaacagat tataaaaaag tatggcttag aaaaccattc
    10801 cacggagctg aaaatcatac tggaaaaacc attgtcttcg gtcatacacc ggtctatggt
    10861 ttgttacagc aagaacgcgg tacatctgag ctttggacta cagaagatgg caagattgga
    10921 atggatggag gagctgttta tggtggtgtc cttcacggga ttgtctttac agaccaagga
    10981 atgacagaac accactttat cgaaaatgac ggctttgtcg ctgaagatta gtactcctag
    11041 cagggtatgg tcttgtcaaa atatcaaaaa caatttataa taaatagata ccctgaaagg
    11101 aagagaatca tgaacttaga agaattgaaa aaacgacagg ggaagatccg taacttctct
    11161 attatcgccc atattgacca cgggaaatca actctagcag accgcatttt ggaaaaaaca
    11221 gagacggttt caagtcgtga aatgcaggcc cagcttttgg atagcatgga tctagagcgg
    11281 gaacgtggaa ttactattaa gttgaatgcc atcgagttga attacactgc aaaagatggg
    11341 gaaacttata ttttccactt gattgacaca ccagggcacg ttgactttac ctatgaagtt
    11401 tcacgttcgc tagctgcctg tgagggtgct attttggtgg tcgatgcggc tcaaggaatt
    11461 gaggctcaaa ctcttgccaa cgtttatctg gctttggata atgatttgga aatcatgcct
    11521 atcattaata aaattgacct gccggctgca gatccggagc gcgtgcgtac agagattgaa
    11581 gatgtgattg gtttggatgc cagtgaagca gttttggctt ctgccaaggc tggtattggg
    11641 attgaagaaa tcctcgagca aattgtagaa aaagtaccag caccaacggg tgatgtgacg
    11701 gcgccactta aggccttgat tttcgactct gtttacgatg cttaccgtgg ggttatcctc
    11761 caagtgcgtg tcatggacgg agtggtcaaa cctggtgata agattcagct catgagcaat
    11821 agtaagacct ttgatgtggc cgaagtcggt atttttacac caaaagcggt tggtcgtgat
    11881 ttccttgcga ctggtgatgt tggttacatt gcggcgtcta tcaagacggt tcaggatact
    11941 cgtgtgggtg ataccgttac cttggcaacc aatcctgcgg cagaaccatt acatggttat
    12001 aagcagatga atcctatggt ctttgcgggt ctctacccaa tcgaatcaaa caagtacaat
    12061 gacctgcgtg aagcgcttga aaaattgcaa ctgaatgatg ctagtcttca gtttgaacca
    12121 gaaacatctc aggcacttgg atttggtttc cgttgtggat ttcttggact tctccatatg
    12181 gatgttattc aggaacgttt agagcgtgag ttcaatattg acctcatcat gacagctccg
    12241 tctgttattt acaaagttaa tttgaccgac ggtgagtcta tggatgtgtc taacccatct
    12301 gagtttccag acccaactaa gattgcgacc attgaagagc cttatgtcaa ggcgcaaatc
    12361 atggtaccac aggagttcgt cggagcagta atggagctag ctcagcgtaa gcgtggggac
    12421 tttgtgacta tggactatat tgatgataac cgtgtcaatg ttatctatca aattcctctt
    12481 gctgaaattg tctttgactt ctttgataaa cttaagtctt cgacacgtgg ttatgcaagc
    12541 tttgactacg aattgtcaga atatcgccca tctaagctgg tgaaaatgga tatccttctc
    12601 aatggagata aggtggatgc cctcagcttt atcgttcaca aggactttgc ctacgaacgt
    12661 gggaaactca tcgttgataa actcaagaaa atcatccctc gccaacaatt tgaagttcca
    12721 atccaagcgg ctattggaca caagattgtc gctcgtactg atatcaaggc ccttcgtaag
    12781 aacgtgcttg ctaaatgtta tggtggtgac gtttctcgta agcgtaaact ccttgaaaaa
    12841 caaaaagctg gtaagaaacg catgaaatcc atcggatcag ttgaagttcc gcaagaagcc
    12901 ttcctcagcg tcttgagtat ggatgaagaa taagttatca gaaatgcctt tccttgttca
    12961 caggaaattt atataaaaat caatagtaaa gcaggcttag atggtctgct tttttagttt
    13021 ttatactctt cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgta tgtatggtta
    13081 ctgacttcgt cagtttcatc tacaacctca gaa
//
LOCUS       DAVKGC010000052        13096 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKGC010000052 DAVKGC010000000
VERSION     DAVKGC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.052"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            42..1388
                     /gene="celB"
                     /locus_tag="VW049_001240"
     CDS             42..1388
                     /gene="celB"
                     /locus_tag="VW049_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEU9432366.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            1767..1943
                     /locus_tag="VW049_001241"
     CDS             1767..1943
                     /locus_tag="VW049_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432367.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            2345..4558
                     /locus_tag="VW049_001242"
     CDS             2345..4558
                     /locus_tag="VW049_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9432368.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(4780..5256)
                     /locus_tag="VW049_001243"
     CDS             complement(4780..5256)
                     /locus_tag="VW049_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9432369.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLQDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            5479..8679
                     /locus_tag="VW049_001244"
     CDS             5479..8679
                     /locus_tag="VW049_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU9432370.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTIGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDNKIGIAKVRIIEESGKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEW
                     LHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVEQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKSPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKHGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYML
                     IPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQVAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            9059..9444
                     /gene="tnpA"
                     /locus_tag="VW049_001245"
                     /pseudo
     CDS             9059..9444
                     /gene="tnpA"
                     /locus_tag="VW049_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9823..10452)
                     /locus_tag="VW049_001246"
     CDS             complement(9823..10452)
                     /locus_tag="VW049_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9432371.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(10462..11463)
                     /locus_tag="VW049_001247"
     CDS             complement(10462..11463)
                     /locus_tag="VW049_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9432372.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(11494..12135)
                     /locus_tag="VW049_001248"
     CDS             complement(11494..12135)
                     /locus_tag="VW049_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9432373.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(12154..12969)
                     /locus_tag="VW049_001249"
     CDS             complement(12154..12969)
                     /locus_tag="VW049_001249"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9432374.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         4095 a         2239 c         2786 g         3976 t
ORIGIN      
        1 agaaagtaaa aattattatg ttttattaag gaggtctatt tatggatcaa caaaacgggt
       61 tgtttggttt tcttgaaaac catgttatgg gaccaatggg caaacttgct cagtttaaag
      121 tagtacgtgc tatcacggct gcaggtatgg ctgctgtacc atttactatt gtaggatcaa
      181 tgtttttggt attcagtatt ttgccacaag ctttctcatt ttggccaatt gtggcagata
      241 ttttctctgc ttcatttgat aaattcacat cactttacat ggttgcaaac tatgcgacta
      301 tgggttctct atctctttat ttcgttctat cacttgcata tgaattgaca aaaatttatg
      361 cagaggagga agaactcaat atgaatcctc ttaatggtgc cttgcttgcc ttgatggctt
      421 ttgtcatgac agtaccgcaa atcatttttg atggtggaat gatgaagact gtgacaagtc
      481 taaaagaagg agcagtaatt gcagatggat gggcaatggg aaatggagtc gcacgttttg
      541 ggacaacagg gatttttacc gcaatcatta tggcaattgt gactgttctt atttatcgta
      601 tgtgtgttaa acataattgg gttattaaaa tgcctgaagc tgttccagaa ggagtttctc
      661 gtggatttac cgctttggtt ccgggatttg ttgttgcatt tgttgttatc tttatcaacg
      721 gtcttcttgt agcaatggga acagatattt ttaaagtcat tgcaattcca tttggttttg
      781 tatccaatct gactaattcg tggattggtt taatgattat ttatctattg actcaactac
      841 tttggattgt aggtatccac ggtgcgaaca ttgtttttgc atttgttagt ccaattgctc
      901 ttgctaacat ggctgaaaat gctgctggcg ggcacttcgc tgttgcaggt gaattttcta
      961 atatgtttgt aattgcaggt ggttctggtg caactttagg actatgttta tatattgctt
     1021 ttgcctctaa atctgaacag cttaaagcaa taggacgagc atctgtagtt ccagccttat
     1081 ttaatattaa tgaaccatta atttttggat tacctattat ctataatcca gccttggcta
     1141 taccatttat tttagcacca atggttactg ctactattta ttacgtagcg aattctctaa
     1201 actttattaa gccaattatc gcacaggttc catggccaac tccagtaggg attggagctt
     1261 tcttagggac agcagatctt cgagctgtat tagttgctct agtatgtgca tttgcagcat
     1321 tcttagtcta tcttccattc atccgtgtat atgatcaaaa attggtgaaa gaagagcaag
     1381 gtatctaata agtaaaaaag tatactcgat gatagaatcc atatagttta aataatgagc
     1441 gttaacatta gcagtgaaga ggtcttttta agatattctt ctctgctttt tcatattttt
     1501 tcgctatgta gggtatgaag caaaatagaa agtagttcga atagttgata aacagggaat
     1561 tgctgttatt taaaaaaaca ataaagtaaa gtatgttatc gtagaatacg atacaatagt
     1621 aaaaaagcaa aaagaatgcg ttttatattt gggtttgagg tttataaaat aggataagcg
     1681 tgctgaaaac ttagatatga caaacaaaat aaaattttta aaagctacag agaaacatgg
     1741 tataatgtat ctaaaggagg tagcatatgt ccttagatat agataaagaa aagatgacaa
     1801 tcatgggaat agcttttgaa aaccgatccg tttttaagag tgtttggtat gctttaagta
     1861 caaatatgat tgaaggatgg cgacctacgg ttagtgatgt tgaaaaatta cgagatgaag
     1921 ctcttgctct ggggatgact taatgaagcg caactgttat gagtcttatg actatgttga
     1981 tccggataat ttatataccg atccgggttc ttcagttcta agaaataagc aggaaggaaa
     2041 aaagcacgcg agcttgaata tcgtatgatt gctagtaaga gtttgaaatt atttatcaat
     2101 cctatcttag ttaatagttt aaaagtagtt tgtttcacaa ctgcataagc ttagaaaagt
     2161 tgaaaagtga taactcttta ataaactctt ggtttgttaa aagatatttt gtccactaca
     2221 tttttttcaa ttgactatta tttgtcatag atataacaga tagaacattg cattcagaat
     2281 aatgaaaaac gcatcaaaaa tggtgcgttc tgtttttatg ctaccataaa aggaggaaaa
     2341 caaattgaaa atttttaagg gagagtttta tcgaatctct gtattaacag acaagctagt
     2401 aaggttagaa tactctcaaa ctggaagttt tgaggataga acgacacaac ttatctataa
     2461 tagagatttt ggccaaattt cgttagatta tatcgagaca tcaaacgtac tagatattat
     2521 gacggactat tttcatctgc actttaataa aggagaattt aacgccgaaa atttatttat
     2581 agaattaaaa ggaaattttg ccgtatatgg tagtcgctgg tattttggtg aatctattga
     2641 aacgttaaaa ggaacagctc ggactctgga taaggcagat ggagcaatct cgttagaaga
     2701 tggaattatt agccgaaatg gtatagcctt attggatgat tctcaaggat ttatttggga
     2761 tgaacaatct ggttatattg agagagaaaa tcaaattgac ctgtatttct ttgcctatgg
     2821 gcatgattat agaggagcaa tcagagattt ttaccatttg actggttcaa cacccttgtt
     2881 gccaagatat gctttaggca attggtggag tagatattgg ccttatacgt cggatgaata
     2941 cttggattta atagacagat ttgaaacaga gaaaattcca ttatctatcg gtgtgttaga
     3001 tatggattgg catataactg acattccagc tcgctttgga agtggctgga caggatatag
     3061 ttggaataga aacttaatac caaatccaga acagttattg caacaacttc atgatagaaa
     3121 gctaaaactc tccttaaatg tccatcctgc tgatgggata cgggcttatg aagaagctta
     3181 tcctcaaatc gcaaaacggt tggggttaaa tgtagaacta gaagaacctg ctatttttga
     3241 tttttttaat ccctctttta gggaagccta ctttaaagat gttcattatg aactagaaaa
     3301 gcagggagta gatttttggt ggattgactg gcaacaaggg acacaaggta tgctagatcc
     3361 actttggctt ttaaaccatt atcactatca ggatagttgt aaaaatgcag aaggtggttt
     3421 gattttatca agatatgcag gtcctggtag tcaccgctac cctgttggtt tttcagggga
     3481 tactattatt agttggaatt ccttaagatt tcaaccctat tttacagcga cagcatctaa
     3541 tatcggttat agttggtgga gtcatgatat cggtggacat atgttggggg attatgacga
     3601 agagctacaa actagatggc tacagtttgg tgtttttagt ccgattactc gattacatag
     3661 ttctagaagt ccttttaata gtaaagaacc ttggtttttt tcagaaacaa catctaagat
     3721 tatgaagaaa taccttcgtt tgagacatca gatgattccc tatctatata ccatgaatgt
     3781 acagacacat gaggaaggtg ccccattaat cagtccaatc tattatttct atccagagaa
     3841 tgatgagagc tataatgttc caaaccaata cttttttgga acagaactga tggtggctcc
     3901 cattgtagaa aagatggatt tgacattcca atctgcaaaa gtagatgtat ggttccctga
     3961 aggtgaatgg tatgacttct tttcagagaa aaaatacaca ggtggtgtga agttaagtgt
     4021 ttatagggac atctcgacta cgcctgtgtt tgcaaaaagt ggtgcaatca ttcccttggt
     4081 tggttctgag ataggtatgg gtgttgattt acctgaagtt gtagattggt atgtattccc
     4141 aggaaaacaa cattcttttg aaatgcttga agatcaaaat ggtcaaagat ataaaacaag
     4201 attatcaatc gattgggaaa tgggaatggt agagttagca ttacaaggag attctagtat
     4261 cgttccaagc aatagaaaac atagaattca ttttaaagga acgaatgtgt ctataattga
     4321 attgccaaat aagaatgata cagctaaatt tgaatggaaa gataataaaa ggacatcttt
     4381 aaatgacgaa gtttttagac tactaaagac agcttctctt ccatatgaat taaaagatag
     4441 attgttaaat caattcatca atgcgaaaaa ctctcatgac ttaatgaata tcttgcatca
     4501 tcaggataag gaattgagag ggcgtttgtt ggaaatgata tttactagcc aaaactaatt
     4561 gaaataatta gaaagtcatt tgtttagtgt atatgaagat tctgtatggt attcgttatc
     4621 actggtcaca ttgaaattgt gaaataagca cctacaacat agactttaca acacttcatg
     4681 tttcatatca tggggtgttt ttctatttaa gtcaaagccc atcaaaaaat ctcactattc
     4741 atcaggttaa aggaaaccta atcatagtga gattgtgaat tatagtagag tttgtatcgc
     4801 ctcttcaatt tgttttgggt ctgtttttga agaaaagcgt tcaaagactt gcccatctcg
     4861 accgatgaga aacttagcga aattccattc gactcgtttt cctagtgggc cggatttctg
     4921 gtcttgtaac cagacataga gagggtctgc ttccttaccg ttgaccttaa tcttggcaaa
     4981 acgtgggaag gtggtttgaa aatgtaggct acagaaggcg ttgatttcct ctgcgctgcc
     5041 gggtgcttgt cccataaact gattgcaagg gaaatccaat atttcgaagc cctgatcttg
     5101 atagcgttca tagagttctt gaagtccctg gtactggggc gttaaaccac atccagtagc
     5161 agtgttgaca atcaagagaa ctttgccacg atagctatcc aagggagttg cttggttgtt
     5221 ttggttcaaa acggaaaaat cataaagtga agtcatgagg gcctcttttc tttttctaca
     5281 ttgtaacagt ttttactttt ttcgtcgagt cggatggaag atattgttga agaataagcc
     5341 ttcaatatga tagaaaccgt ttacaaataa ctttaaaaat ggtatgattt aattaaaccg
     5401 gtttacttag ttgctataaa tctctaattt gagatttagt tcctattgat tttacaatat
     5461 gtttattgga gtgtatacat gcaaacaaaa acaaagaagc tcattgtgag tttgtcttca
     5521 cttgttttat caggattttt attaaaccat tatatgacaa ttggagcgga agaaacgact
     5581 acgaatacca ttcagcaaag ccagaaggaa gttcagtatc agcaaaggga tacaaaaaat
     5641 ttagttgaaa atggtgattt tggtcagacg gaggacggaa gcagtccgtg gacaggaagc
     5701 aaagctcagg ggtggtcagc ttgggtagac cagaagaata gtgcagatgc ctcaactcga
     5761 gtcattgagg ctaaggatgg ggctatcact atctcaagcc atgagaaatt aagggcagcg
     5821 cttcaccgta tggttcctat tgaagctaag aaaaagtata aactgcgttt caagattaaa
     5881 acagataata aaatcgggat tgccaaagtt cgtatcattg aggaaagtgg taaggacaag
     5941 cgattgtgga attctgcaac gacgtcagga acaaaggact ggcagaccat tgaagcagac
     6001 tatagcccga ctttagatgt tgataaaatc aagctggagt tattctatga aacaggaact
     6061 gggactgttt cctttaagga tattgagctg gtagaggtag cagaccagct ttctgaggat
     6121 tctcaaacag ataaacagct tgaggaaaag attgatttac caattggaaa aaaacatgtt
     6181 ttttctcttg cggactatac ttataaggta gaaaatcctg acgttgcttc agtcaaaaat
     6241 ggaattttag aacctcttaa ggaagggaca accaatgtca ttgtcagtaa agatggcaag
     6301 gaagtgaaaa agattccttt gaagattcta gcctctgtta aggatgcata cacagcccgt
     6361 ttggatgact ggaatggcat catcgctggg aatcaatact atgattctaa aaatgaacag
     6421 atggccaaat taaaccagga attggaagga aaggtagctg atagcctatc tagtatttca
     6481 agtcaggcgg accgcaccta tttgtgggaa aaattttcaa attataaaat gtctgcaaat
     6541 ctgactgcca cttatcggaa attggaggag atggccaagc aagtgaccaa tccttcttct
     6601 cgttattatc aagatgaaac tgtcgttcga acagtcaggg attccatgga atggctgcat
     6661 aaacatgtct acaatagtga aaagagcatt gttgggaact ggtgggatta tgaaatcggt
     6721 acacctcgtg ccatcaacaa taccttgtct ctgatgaaag aatacttctc tgatgaggaa
     6781 attaaaaaat atacagatgt gattgaaaaa tttgtaccag atcccgaaca tttccgaaag
     6841 acgactgata acccattcaa ggctctaggt ggaaacttag ttgatatggg aagggtaaaa
     6901 gtaatagctg gtttactgcg taaggatgat caagaaattt cttctaccat tcgctcgatt
     6961 gagcaagtgt tcaagttggt agagcaaggt gaaggttttt atcaagatgg atcctatatc
     7021 gaccacacca atgttgccta tacgggtgct tatgggaatg ttttgattga tggcctgtct
     7081 caactgttgc cagtcattca aaagaccaag agtccaatcg ataaagataa aatgcaaacc
     7141 atgtaccact ggattgataa atcgtttgct cctttgctgg tgaatggaga gttgatggat
     7201 atgagtcgtg gacgctcgat cagtcgtgca aatagcgagg ggcacgtggc cgcagtagaa
     7261 gtactaagag ggattcaccg aatagcggat atgtctgaag gagaaaccaa acaacgtttg
     7321 cagagtcttg tgaagaccat tgttcaatcg gatagttatt atgatgtctt taagaatttg
     7381 aagacttata aggatatcag tttgatgcaa tccttgttaa gtgatgcagg agtcgcaagt
     7441 gttccaagaa caagttacct atctgccttt aacaagatgg ataaaacagc catgtacaac
     7501 gcagagaaag ggtttggatt tggcttgtca ctcttttcca gtcgtacctt gaattacgaa
     7561 cacatgaaca aggaaaataa acatggttgg tatacgagtg atgggatgtt ctatctttac
     7621 aatggcgatt tgagtcacta tagcgatggc tactggccaa cagttaatcc atataagatg
     7681 cctggtacaa cagagacgga tgctaagaga gcggatagcg atacaggtaa agttttaccg
     7741 tctgctttcg ttggaacgag caaactagat gatgccaatg cgacagcaac catggatttc
     7801 accaactgga atcaaacatt gactgctcat aagagctggt ttatgctaaa ggataagatc
     7861 gcctttttag gaagcaatat ccaaaacact tcaacagata ctgctgcaac tacaattgac
     7921 cagagaaaac tggaatcaag taatccatat aaagtctatg tcaatgataa agaagcctcc
     7981 cttacagaac aagaaaagga ttatcctgaa acccaaagtg tctttttaga atcgtccgat
     8041 tcgaaaaaga atattggtta ctttttcttt aagaagagtt caatcagtat gagtaaggct
     8101 ttgcaaaagg gagcctggaa ggatatcaat gaaggacagt cagacaagga agttgaaaat
     8161 gaatttctta cgattagtca ggctcataag caaaatggag attcttatgg ctatatgctt
     8221 atccctaacg tggatcgtgc caccttcaat caaatgataa aagagttaga aagtagcctc
     8281 atcgaaaata acgaaaccct tcagtctgtt tatgatgcta aacaaggagt ttggggcatt
     8341 gtgaaatatg atgattctgt ctctactatt tccaaccaat tccaagtttt gaaacgtgga
     8401 gtctatacta ttcgaaaaga gggggatgaa tataagattg cctactataa tcctgaaacc
     8461 caggaatcag ctccagatca ggaagtcttt aaaaagctag agcaagtagc tcagccacaa
     8521 gtacagaatt caaaagaaaa ggaaaaatct gaagaggaaa agaaccattc ggatcaaaag
     8581 aatctccctc agacaggaga aggtcagtca atcttggcaa gtctagggtt cttgctactt
     8641 ggggcgtttt atctattccg tagaggaaag aacaactaat ttgttcatca tctagatgaa
     8701 ttacatgaaa ctgtggaaaa tagaaaaaat ccgaagaagg ccaaatatct tcggattttt
     8761 cttttaaaaa agataagttg atacttatcc ttttttactt cattatgtga ctatagcgtt
     8821 ttgaaactag aataggacgt cataactgct aaaagatttc tataaattca tttgattttc
     8881 ctaatcaatt tgttcgtatc ctatttcact ccactatatt agtccgtctc gctccgtgag
     8941 gtgcgagaca aaacaaccac ccgctatacg ggtgcgcgtc gaaggttata ccaaaaaaac
     9001 tccaaacgcg atacaataaa ggtgttcaat ccaattgtaa agcgaaagga gaaaaaatat
     9061 ggcacaaaag actcatagtt tatcatacac aaagtggatg tgttctatca tattgtgttc
     9121 acccctaagt atagacgaaa agtcatctat aatcaatata gaagtagttt aggcgaaata
     9181 tttcatcgct tgtgtagtta taaaggtgtt gaaattatcg agggtcactt aatgccagac
     9241 catgtacaca tgttagtcag tattccacta aggctaagtg tttcaagttt tatggggtat
     9301 ttaaaaggca aaagtgcact catgatgttt gacaaacacg ccaatctcaa gtacaagttt
     9361 gggaatcggc atttctgggc ggaaggttat tatgtaagta cagtagggct taataaagcc
     9421 acacttaaga aatatagtca agattaaaga aattatccag tggatgattt cttcacgagt
     9481 atgaaaattt gagaactagt aaagtatgat atatagtaaa atgaaataag aacaggacaa
     9541 aatcgatcag gacagtcaaa tcgattttta acaatgtttt agaagtagag gtgtactatt
     9601 ccagtttcaa tctactatag cactagataa attgagtgta aaagaatatg aggatccctt
     9661 tagggatagt ggtaagtaat accaaagtct cttaaagagg caagtgacga gtcaagagca
     9721 ataaggcttg aacaacgtga aagccagcgt ctttaggcgc tggctgatga tttgggctta
     9781 tagctctgag ataaaccacc cgttagacgg gtagttatga ttttatctga gtgtaacata
     9841 ctgttgggca atctcgctga tgcggtcaaa gttgccttgg gaagcgagtt tattgagttc
     9901 gccaccaatt ccaacggcat ctgcaccagc agcgaaccat tgagggatgt tgtttaggcc
     9961 gactcctccg gttaccatta cggaaacttg tgggatcggt gccttgactg cagagatata
    10021 tgctggactg agagtactac ctgggaagag tttgatgatt tcactaccgg cttcaagtgc
    10081 agtcgtgatc tctgtgaggg taatacagcc tggaatgtac ggtgtgctgt agagattgca
    10141 cattttcgca gtttcagcat ggaaagatgg agaaacaacg taatttgctc cagctagaat
    10201 ggcatctcta gcagttacgg catcaagcac agtacctgca ccgatacaaa cactctgatc
    10261 gtcctgatac aagtctacaa gttccttgat gatttgtcct gcatactgat tggtataggc
    10321 gatttcaata gctttgatac cgcccttgat acaagcaatc gaggcttgta gtccttcttc
    10381 ctttgtattt cctcgaatga cagcgacaat tttctgtttt tttagttcaa taatcgtatc
    10441 tgatttggtc atgtaattct cctaacgaat gatatcttgt gcatttgcca gtaaattttc
    10501 aatactagtt gcggaagtgg agagatggtc tcctggaaga gtgcatttga gagttgcgct
    10561 cgcaactgca aagtcaatgg tagtttttag ggaggaatga tggagtagtt ggtaaagggc
    10621 accagatata aaggcatccc cgctaccaat tcgttgatag actgcagttt ttagttggac
    10681 agactcttca aatcgttctt ccagagcata ggcttgatag acatttttgt cttgctcatc
    10741 actagatcgg agggtatgga aaatggcctt gaaaccatag gcttctttta aaagtcgcat
    10801 gcgattttcc acctcttcta ggctagcact gtctcttgga aacatctcta gattttggtc
    10861 atcaatcatg agaggatcaa taccgaagca atagtcagta aaacgtgcaa acttagaaaa
    10921 ttcatacttg gcttctagga ctgaaatcat ctttgttctc agattgagat ccattgaaac
    10981 gacaattcct cggcgcttgg cttcttccaa gagtaggaga aggatcgcac ggacctcttg
    11041 accgatagct atggtgattc cactaaaatg aaaatggcta atcccctgaa agagagaatc
    11101 catatctagc atgtttggcc gaatctggct gatactcgta tgcttacgat cgtagaaaac
    11161 ttcactttga cgacaaccaa agccgttctc caaatagtag aggccgattc gatcgccaag
    11221 ccgacagatt gaactggtat cgatttggtg ctgtttcaag aatgtgagaa aacgatctcc
    11281 aatctcgttg gcagggagtg cggtaaagac tttcgttgag atacccaggg cttgcaaatt
    11341 acaagcgatg ttaatttctg atccgccaaa ataagtcgaa ctggcaacat gatcgccgat
    11401 actggtggca tctaatggtg agattcgaat taatggttcg ccaaacagta atattttcgt
    11461 catccattag ttcctttctg gtttagaata gctttacttg gctaatactt ctttcaaata
    11521 agcagcgact tcatcatctt ggcagttttc aaagaagtat tcttggaagt atttgccaga
    11581 gatggtgtct ttgaggaagt cttggtcgat tgttttaagg atggtcatca aatcattgtg
    11641 ggtgatttgt ttcacctcgt ttaagatacg agcgttgcgt tgttcaggga ttacacgttc
    11701 tcttgggtag ccaccgccca tttcttcagc aaataagcgt tcaaacatca atttgagggt
    11761 cagttctgcc ccccagccaa atcctttggc ataagggata gacaaggcgt taccaccatt
    11821 gatttgagaa taaaggtaag cgtcagttgg gtccactgct agaccacaga caacaccagg
    11881 gaagctgttt aaagcaagca tagcccctac acccgtacca cagccggtaa caacaaagtc
    11941 aactgccttt gtatttaaaa ggatggcagc cattagtccg ttctgcacat aagttaattg
    12001 actttctcct tcttctccac gcataccata gttaaataat tggtagtctt ttttatctgt
    12061 cgcttctttt agactatcgt aaataatgtg attcttgcta gcttgactat tttcattgat
    12121 taatgcgatt ttcataagat cttctttcta tttttactca ggttgttttc cgatgtaggc
    12181 taagataccg ccatctacat ataggatgtg gccattgaca aaattgctgg catcactagc
    12241 gagaaagaca gcagggccca tcaaatcttc aggatttccc caacgtgcag caggtgtttt
    12301 tgcaatgatg aactggtcaa atgggtggcg agaaccatct tcttgcaact cacgaagagg
    12361 tgctgtttga ggagtggcaa tataacccgg tccaattccg ttacattgga tattggctcc
    12421 accgtattca gacgcaatgt tgcgggtcaa cattttcaag ccccctttag cagcagcata
    12481 agcgctaact gtttcacgtc ccagttcgct catcatcgaa caaatattga taatctttcc
    12541 atgccctttc tttatcatag aaggaataac tgcctttgaa acgataaatg gtgcgtttaa
    12601 gtcaatatcg atgaccttac ggaaatcagc ggcgctcatt tcgcacattg gaactcggcg
    12661 gataatacca gcgttattaa cgaggatgtc aatgacacca acctcttgtt cgatttgctt
    12721 gaccatggct tggataccgt cctcgtctgt cacgtcacag acatatccat gggcttcgat
    12781 gccaacttca cgataagctt caatcccttt attgaccaga tcttgattga tatcgttaaa
    12841 gacaatagta gcgccggctt cagcgtagga tttggcaata gcaaatccaa ttccataaga
    12901 agcgccggtg atgagagcaa tttttccttt taaagaaaac tgctcaattg agaatgatgt
    12961 atttgtcatt tgatacttcc tttcaaattt gttaagactt ctctataatc aaattatatt
    13021 ctagtaaacc ggttttgtca ataaataaat atttttattt ataaaaagat ttattttcct
    13081 taaaaatgga atattt
//
LOCUS       DAVKGC010000053        12544 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKGC010000053 DAVKGC010000000
VERSION     DAVKGC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12544
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.053"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..1074
                     /gene="dnaN"
                     /locus_tag="VW049_001250"
     CDS             <1..1074
                     /gene="dnaN"
                     /locus_tag="VW049_001250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9432375.1"
                     /translation="ISSKNAIPILSTVKIDVTNEGITLIGSNGQISIENFISQKNEDA
                     GLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQY
                     PRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATD
                     SHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENIS
                     FYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDG
                     VVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVR
                     PFTLVPADTDEDFMQLITPVRTN"
     gene            1139..1333
                     /locus_tag="VW049_001251"
     CDS             1139..1333
                     /locus_tag="VW049_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9432376.1"
                     /translation="MYQVGNFVEMKKPHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKINKIID"
     gene            1417..2532
                     /gene="ychF"
                     /locus_tag="VW049_001252"
     CDS             1417..2532
                     /gene="ychF"
                     /locus_tag="VW049_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9432377.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2603..3172
                     /locus_tag="VW049_001253"
     CDS             2603..3172
                     /locus_tag="VW049_001253"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9432378.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTHVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3173..6682
                     /gene="mfd"
                     /locus_tag="VW049_001254"
     CDS             3173..6682
                     /gene="mfd"
                     /locus_tag="VW049_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9432379.1"
                     /translation="MVTLLDLFSENDQIKKWHQSLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKEIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQGYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRIKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            6740..7006
                     /locus_tag="VW049_001255"
     CDS             6740..7006
                     /locus_tag="VW049_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9432380.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            6999..7367
                     /locus_tag="VW049_001256"
     CDS             6999..7367
                     /locus_tag="VW049_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9432381.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7487..8755
                     /locus_tag="VW049_001257"
     CDS             7487..8755
                     /locus_tag="VW049_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9432382.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8752..10029
                     /gene="tilS"
                     /locus_tag="VW049_001258"
     CDS             8752..10029
                     /gene="tilS"
                     /locus_tag="VW049_001258"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9432383.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10033..10575
                     /gene="hpt"
                     /locus_tag="VW049_001259"
     CDS             10033..10575
                     /gene="hpt"
                     /locus_tag="VW049_001259"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9432384.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10591..>12544
                     /gene="ftsH"
                     /locus_tag="VW049_001260"
     CDS             10591..>12544
                     /gene="ftsH"
                     /locus_tag="VW049_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9432385.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         4310 a         1891 c         2561 g         3782 t
ORIGIN      
        1 attagttcta aaaatgccat tcctatttta tcaacagtaa aaattgacgt gaccaatgaa
       61 ggtattactt taattggttc aaatggtcaa atttcaattg aaaattttat ttctcaaaaa
      121 aatgaagatg ctggtttgtt aattacttct ttaggttcga tccttcttga agcttctttc
      181 tttatcaatg tagtatctag tttacctgat gtaactcttg attttaaaga aattgaacaa
      241 aatcaaattg ttttaaccag tggcaaatca gaaattaccc taaaaggaaa agatagcgaa
      301 caatatccac gaatccaaga aatttcagca agcactcctt taatacttga aacaaaatta
      361 ctcaagaaaa ttattaatga aacagccttt gctgcaagta cacaagagag tcgtccgatt
      421 ttaacaggtg tccacttcgt attgagtcaa cacaaagagt taaaaacagt tgcaacagac
      481 tctcatcgcc taagccagaa aaaattgact cttgaaaaaa atagtgatga ttttgatgtc
      541 gtaattccta gccgttctct acgcgaattt tcagcggtat ttacagatga tatcgaaact
      601 gtagagattt tctttgccaa taaccaaatc ctctttagaa gcgaaaatat tagcttctat
      661 actcgtctcc tagaaggaaa ctatcctgat acagatcgct tgattccaac agactttaac
      721 actactatta cttttaatgt ggtaaactta cgccagtcaa tggagcgtgc ccgtctttta
      781 tcaagtgcga ctcaaaatgg tactgtgaaa cttgaaatta aggatggggt tgttagcgcc
      841 catgttcact ctccagaagt tggtaaagta aacgaagaaa tcgatactga tcaggttact
      901 ggtgaagatt tgaccattag tttcaaccca acttacttga ttgattctct taaagcttta
      961 aatagcgaaa aggtgactat tagctttatc tcagctgttc gtccatttac tcttgtgcca
     1021 gcagatactg acgaagactt catgcagctc attacaccag ttcgtacaaa ttaagtgaaa
     1081 gaggttgagc ctggctcgcc tcttttatga tataatcgaa aaagaaaagg agagtagtat
     1141 gtatcaagtt ggaaattttg ttgagatgaa aaaaccacac gcttgtacaa tcaagtcgac
     1201 tggtaaaaag gctaatcgtt gggaaattac acgtgtagga gcagatatca aaataaaatg
     1261 cagtaattgt gagcatgttg tcatgatggg gcgatatgat tttgagcgaa aaataaataa
     1321 aattattgac tgagaaccct tagttagagg gttagcactt tatctctttt tgtgttataa
     1381 tattagggat tgaaatgaaa acggagaatg agaaatatgg ctttgacagc aggtatcgtt
     1441 ggtttgccaa acgttggtaa atcaacacta tttaatgcaa ttacaaaagc aggagcagag
     1501 gcagcaaact acccatttgc gactattgat ccaaatgttg gaatggtgga agttccagat
     1561 gaacgcctac aaaaactaac tgaaatgata actcctaaaa agacagttcc cacaacattt
     1621 gaatttacgg atattgcagg gattgtaaaa ggagcttcaa aaggagaagg gctagggaat
     1681 aaattcttgg ccaatattcg tgaagtagat gcgattgttc acgtagttcg tgcttttgat
     1741 gatgaaaatg tgatgcgcga gcaaggacgt gaagacgcct ttgtagatcc acttgcagat
     1801 attgatacaa ttaatctgga attaattctt gctgacttag aatcagtgaa caaacgatat
     1861 gcgcgtgtag aaaagatggc acgtacgcaa aaagataaag aatcagtagc agaattcaat
     1921 gttcttcaaa agattaaacc agtcctagaa gacgggaaat cagctcgtac cattgaattt
     1981 acagatgagg aacaaaaggt tgtcaaaggt cttttccttt tgacgactaa accagttctt
     2041 tatgtagcta atgtggacga ggatgtggtt tcagaacctg actctatcga ctatgtcaaa
     2101 caaattcgtg aatttgcagc gacagaaaat gctgaagtag tcgttatttc tgcgcgtgct
     2161 gaggaagaaa tttctgaatt ggatgatgaa gataaaaaag agtttcttga agccattggt
     2221 ttgacagaat caggtgtaga taagttgacg cgtgcagctt accacttgct tggattggga
     2281 acttacttca cagctggtga aaaagaagtt cgcgcttgga ctttcaaacg tggtatgaag
     2341 gctcctcaag cagctggtat tatccactca gactttgaaa aaggctttat tcgtgcagta
     2401 accatgtcat atgaagatct agtgaaatac ggatctgaaa aggccgtaaa agaagctgga
     2461 cgcttgcgtg aagaaggaaa agaatatatc gttcaagatg gcgatatcat ggaattccgc
     2521 tttaatgtct aaaaattaat aaatggtgtc aattaggttg gaaaaaaatt ccaacccttt
     2581 tggcttttga aaggaaaaat aaatgaccaa attacttgta ggcttgggaa atccagggga
     2641 taaatatttt gaaacaaaac acaatgttgg ttttatgttg attgatcaac tagcgaagaa
     2701 acagaatgtc acttttacac acgataagat atttcaagct gacctagcat cctttttcct
     2761 aaatggagaa aaaatttatc tggttaaacc aacgaccttt atgaatgaaa gtggaaaagc
     2821 agttcatgct ttattaactt actatggttt ggatattgac gatttactta tcatttacga
     2881 tgatcttgac atggaagttg ggaaaattcg tttaagagca aaaggctcag caggtggtca
     2941 taatggtatc aagtctatta ttcaacatat aggaactcat gtctttaacc gtgttaagat
     3001 tggaattgga agacctaaaa atggtatgtc agttgttcat catgttttga gtaagtttga
     3061 cagggatgat tatatcggta ttttacagtc tgttgacaaa gttgacgatt ctgtaaacta
     3121 ctatttacaa gagaaaaatt ttgagaaaac aatgcagagg tataacggat aaatggtgac
     3181 cttattagat ttattctcag aaaatgatca gattaaaaaa tggcatcaaa gtttaacaga
     3241 taagaaaaga caactaatac ttggtttatc aacatctact aaggctcttg caattgcaag
     3301 cagtttagaa aaagaagata ggattgtgtt attgacgtca acttatggag aagcagaagg
     3361 acttgttagt gatcttatct ctatcttggg tgaggaactc gtctatccat ttttggtaga
     3421 tgatgctcct atggtggagt ttttgatgtc ttcacaggaa aaaattattt cacgggttga
     3481 agccttgcgt tttttgactg attcatctaa gaaagggatt ttagtttgta atatcgcagc
     3541 aagtcgattg attttaccgt ctcccaatgc attcaaagat agtattgtaa aaatctcagt
     3601 tggtgaagaa tatgatcaac acgcgtttat ccatcagtta aaggaaaatg gctatcgaaa
     3661 agttactcaa gtacaaactc agggcgaatt tagtcttcga ggagatattt tagatatttt
     3721 tgaaatatcc cagttagaac cttgtcgaat tgagtttttt ggtgatgaaa ttgatggtat
     3781 caggtcattt gaagtagaaa cacaattatc gaaagaaaat aagacagaac tcactatctt
     3841 tccagctagt gatatgcttt tgagagaaaa ggattatcaa cgaggacagt cagctttaga
     3901 aaaacaaatt tcaaaaactt tatcacctat tttgaaatca tacctagaag aaattctttc
     3961 aagttttcac caaaaacaaa gtcatgcaga ctctcggaag tttttatctt tgtgctatga
     4021 taagacatgg actgtctttg attatattga aaaagatact ccaatattct ttgatgatta
     4081 tcaaaaattg atgaatcagt atgaagtctt tgaaagagac ttagcgcagt actttacaga
     4141 agaattacag aatagtaaag cattttctga tatgcagtat ttttctgata ttgaacaaat
     4201 ctataaaaaa caaagtccag tgaccttttt ctctaatctt caaaagggtt taggaaatct
     4261 caaatttgac aaaatttatc aattcaatca atatcctatg caggaatttt tcaatcagtt
     4321 ttcttttcta aaagaagaaa ttgaacgata taaaaaaatg gattacacca ttattctgca
     4381 gtctagcaat tcaatgggaa gtaaaacatt ggaggatatg ttagaggaat atcagattaa
     4441 attggattct agagataaga caagtatctg taaagaatct gtaaatttaa tagagggtaa
     4501 tctcagacat ggttttcatt ttgtagatga aaagatttta ttgataactg aacatgagat
     4561 ttttcaaaag aaattaaagc gtcgttttcg aagacaacat gtttcaaatg cagagagatt
     4621 aaaagattac aatgaacttg aaaaagggga ctatgttgtc catcatatcc atgggattgg
     4681 tcaatatcta ggaattgaaa ccattgaaat caaggaaatt catcgtgatt atgtcagtgt
     4741 ccagtaccaa aatggtgatc aaatttctat tcccgtggaa cagattcatc tactgtccaa
     4801 atatatttca agtgatggta aagctccaaa actcaataaa ttaaatgacg gtcattttaa
     4861 aaaggccaag caaaaggtta agaaccaggt agaggatata gctgatgatt taatcaaact
     4921 ctactctgaa cgtagtcagt tgaagggttt tgctttctca gctgatgatg atgatcaaga
     4981 tgcctttgat gatgctttcc cttatgttga aacggatgat caacttcgta gtattgagga
     5041 aatcaagagg gatatgcagg cttctcagcc aatggatcga cttttagttg gggatgttgg
     5101 ttttggaaag actgaagttg ctatgcgtgc agcctttaaa gcagtcaatg atcacaaaca
     5161 ggttgtcatt ctagttccga cgacggtttt agcgcaacag cactatacga attttaagga
     5221 acgattccaa aattttgcag ttaatgttga tgtgttgagt cgctttagaa gtaaaaaaga
     5281 gcagactgca acacttgaaa aattgaaaaa cggtcaagtc gatattttga ttggaacaca
     5341 tcgtgttttg tcaaaagatg ttgtgtttgc tgatttgggc ttgatgatta ttgatgagga
     5401 acagcgattt ggtgtcaagc ataaggaaac tttgaaagaa ctgaagaaac aagtggatgt
     5461 cctaaccttg accgctacgc caatccctcg taccctccat atgtctatgc tgggaatcag
     5521 agatttgtct gttattgaaa ctccgccgac taatcgctac cctgttcaga cctatgtttt
     5581 ggaaaagaat gatagtgtca ttcgtgatgc tgtcttgcgt gaaatggagc gtggaggtca
     5641 aggttactat ctttacaaca aagttgacac aattgttcag aaggtttcag aattacagga
     5701 gttgattcca gaggcttcga ttggatatgt tcatggtcga atgagtgaag tccagttgga
     5761 aaatactcta ttagacttta ttgagggaca atacgatatc ttggtgacga ctactattat
     5821 tgagacaggg gtggacattc caaatgctaa tactttattt attgaaaatg cggaccatat
     5881 gggcttgtca accttatatc agttaagagg aagagtcggt cgtagtaatc gtattgctta
     5941 tgcttatctc atgtatcgtc cagaaaaatc aatcagtgaa gtctctgaaa agagattaga
     6001 agcgattaaa ggatttacag aattgggctc tggctttaag attgcaatgc gagatctttc
     6061 gattcgtgga gcaggaaatc ttttaggaaa atcccagtct ggtttcattg attctgttgg
     6121 ttttgaattg tattcgcagt tattagagga agctattgct aaacgaaacg gtaatgctaa
     6181 cgctaacaca agaatcaaag ggaatgctga gttgattttg caaattgatg cctatcttcc
     6241 tgatacttat atttctgatc aacgacataa gattgaaatt tacaagaaaa ttcgtcaaat
     6301 tgacaaccgt gtcaattatg aagagttaca agaggagttg atagaccgtt ttggagaata
     6361 cccagatgta gtagcctatc tgttagagat tggtttggtc aaatcatact tggacaaggt
     6421 ctttgttcaa cgtgtggaaa gaaaagataa taaaattaca attcaatttg aaaaagtcac
     6481 tcaacgactg tttttagctc aagattattt taaagcttta tccgtaacga acttaaaagc
     6541 aggcatcgct gagaataagg gattaatgga gcttgtattt gatgtccaaa ataagaaaga
     6601 ttatgaaatt ttagaaggtt tgctgatttt tggagaaagt ttattagaga taaaagagtc
     6661 taaggaagaa aattccattt gatatttttc ttctataaaa tagataaaaa tggtacaata
     6721 ataaattgag gtaataagga tgagattaga taaatattta aaagtatcgc gaattatcaa
     6781 gcgtcgtaca gtcgcaaagg aagtagcaga taaaggtaga atcaaggtta atggaatctt
     6841 ggccaaaagt tcaacggact tgaaagttaa tgaccaagtt gaaattcgct ttggcaataa
     6901 gttgctgctt gtaaaagtac tagagatgaa agatagtaca aaaaaagaag atgcagcagg
     6961 aatgtatgaa attatcagtg aaacacgggt agaagaaaat gtctaaaaat attgtacaat
     7021 tgaataattc ttttattcaa aatgaatacc aacgtcgtcg ctacctgatg aaagaacgac
     7081 aaaaacggaa tcgttttatg ggaggggtat tgattttgat tatgctatta tttatcttgc
     7141 caacttttaa tttagcgcag agttatcagc aattactcca aagacgtcag caattagcag
     7201 acttgcaaac tcagtatcaa actttgagtg atgaaaagga taaggagaca gcatttgcta
     7261 ccaagttgaa agatgaagat tatgctgcta aatatacacg agcgaagtac tattattcta
     7321 agtcgaggga aaaagtttat acgattcctg acttgcttca aaggtgataa aatggaaaat
     7381 ttattagacg taatagagca atttttgagt ttgtcagatg aaaagctgga agaattggct
     7441 gataaaaatc aattattgcg tttacaagaa gaaaaggaaa ggaagaatgc gtaaattctt
     7501 aattattttg ttgctaccaa gttttttgac catttcaaaa gtcgttagca cagaaaaaga
     7561 agtcgtctat acttcgaaag aaatttatta cctttcacaa tctgactttg gtatttattt
     7621 tagagaaaaa ttaagttctc ccatggttta tggagaggtt cctgtttatg cgaatgaaga
     7681 tttagtagtg gaatctggga aattgactcc caaaacaagt tttcaaataa ccgagtggcg
     7741 cttaaataaa caaggaattc cagtatttaa gctatcaaat catcaattta tagctgcgga
     7801 caaacgattt ttatatgatc aatcagaggt aactccaaca ataaaaaaag tatggttaga
     7861 atctgacttt aaactgtaca atagtcctta tgatttaaaa gaagtgaaat catccttatc
     7921 agcttattcg caagtatcaa tcgacaagac catgtttgta gaaggaagag aatttctaca
     7981 tattgatcag gctggatggg tagctaaaga atcaacttct gaagaagata atcggatgag
     8041 taaagttcaa gaaatgttat ctgaaaaata tcagaaagat tctttctcta tttatgttaa
     8101 gcaactgact actggaaaag aagctggtat caatcaagat gaaaagatgt atgcagccag
     8161 cgttttgaaa ctctcctatc tctattatac gcaagaaaaa ataaatgagg gtctttatca
     8221 gttagatacg actgtaaaat acgtatctgc agtcaatgat tttccaggtt cttataaacc
     8281 agagggaagt ggtagtcttc ctaaaaaaga agataataaa gaatattctt taaaggattt
     8341 aattacgaaa gtatcaaaag aatctgataa tgtagctcat aatctattgg gatattacat
     8401 ttcaaaccaa tctgatgcca cattcaaatc caagatgtct gccattatgg gagatgattg
     8461 ggatccaaaa gaaaaattga tttcttctaa gatggccggg aagtttatgg aagctattta
     8521 taatcaaaat ggatttgtgc tagagtcttt gactaaaaca gattttgata gtcagcgaat
     8581 tgccaaaggt gtttctgtta aagtagctca taaaattgga gatgcggatg aatttaagca
     8641 tgatacgggt gttgtctatg cagattctcc atttattctt tctattttca ctaagaattc
     8701 tgattatgat acgatttcta agatagccaa ggatgtttat gaggttctaa aatgagggaa
     8761 ccagattttt taaatcattt tctcaagaag ggatatttca aaaagcatgc taaggcggtt
     8821 ctagctcttt ctggtggatt agattctatg tttctattta aggtattgtc tacttatcaa
     8881 aaagagttag agattgaatt gattctggct catgtgaatc ataagcagag aattgaatca
     8941 gattgggaag aaaaggaatt aaggaagttg gctgctgaag cagagcttcc tatttatatc
     9001 agcaattttt caggagaatt ttcagaagcg cgtgcacgaa attttcgtta tgattttttt
     9061 caagaggtta tgaaaaagac aggtgcgaca gctttagtca ctgcccacca tgctgatgat
     9121 caggtggaaa cgattttgat gcgcttgatt cgaggaactc gcttgcgcta tctatcagga
     9181 attaaggaga agcaagtagt cggagagata gaaatcattc gtcccttctt gcattttcag
     9241 aaaaaagact ttccatcaat ttttcacttt gaagatacat caaatcagga gaatcattat
     9301 tttcgaaatc gtattcgaaa ctcttactta ccagaattgg aaaaagaaaa tcctcgattt
     9361 agggatgcaa tcttaggcat tggcaatgaa attttagatt atgatttggc aatagctgaa
     9421 ttatctaaca atattaatgt ggaagattta cagcagttat tttcttactc tgagtctaca
     9481 caaagagttt tacttcaaac ttatctgaat cgttttccag atttgaatct tacaaaagct
     9541 cagtttgctg aagttcagca gattttaaaa tctaaaagcc agtatcgtca tccgattaaa
     9601 aatggctatg aattgataaa agagtaccaa cagtttcaga tttgtaaaat cagtccgcag
     9661 gctgatgaaa aggaagatga acttgtgtta cactatcaaa atcaggtagc ttatcaagga
     9721 tatttatttt cctttggact tccattagaa ggtgaattaa ttcaacaaat acctgtttca
     9781 cgtgaaacat ccatacacat tcgtcatcga aaaacaggag atgttttgat tcaaaatggg
     9841 catagaaaaa aactcagacg tttatttatt gatttgaaaa tccctatgga aaagagaaac
     9901 tctgctctta ttattgagca atttggtgaa attgtctcaa ttttgggaat tgcgaccaat
     9961 aatttgagta aaaaaacgaa aaatgatata atgaacactg tactttatat agaaaaaata
    10021 gataggtaaa aaatgttaga aaacgatatt aaaaaagtcc tcgtttcaca cgatgaaatt
    10081 acagaagcag ctaaaaaact aggtgctcaa ttaactaaag actatgcagg aaaaaatcca
    10141 atcttagttg ggattttaaa aggatctatt ccttttatgg ctgaattggt caaacatatt
    10201 gatacacata ttgaaatgga cttcatgatg gtttctagct accatggtgg aacagcaagt
    10261 agtggtgtta tcaatattaa acaagatgtg actcaagata tcaaaggaag acatgttcta
    10321 tttgtagaag atatcattga tacaggtcaa actttgaaga atttgcgaga tatgtttaaa
    10381 gaaagagaag cagcttctgt taaaattgca accttgttgg ataaaccaga aggacgtgtt
    10441 gtagaaattg aggcagacta tacctgcttt actatcccaa atgagtttgt agtaggttat
    10501 ggtttagact acaaagaaaa ttatcgtaat cttccttata ttggagtatt gaaagaggaa
    10561 gtgtattcaa attagaaaga ataatcttta atgaaaaaac aaaataatgg tttaattaaa
    10621 aatccttttc tatggttatt atttatcttt ttccttgtga caggattcca gtatttctat
    10681 tctgggaata actcaggagg aagtcagcaa atcaactata ctgagttggt acaagaaatt
    10741 accgatggta atgtaaaaga attaacttac caaccaaatg gtagtgttat cgaagtttct
    10801 ggtgtctata aaaatcctaa aacaagtaaa gaaggaacag gtattcagtt tttcacgcca
    10861 tctgttacta aggtagagaa atttaccagc actattcttc ctgcagatac taccgtatca
    10921 gaattgcaaa aacttgctac tgaccataaa gcagaagtaa ctgttaagca tgaaagttca
    10981 agtggtatat ggattaatct actcgtatcc attgtgccat ttggaattct attcttcttc
    11041 ctattctcta tgatgggaaa tatgggagga ggcaatggcc gtaatccaat gagttttgga
    11101 cgtagtaagg ctaaagcagc aaataaagaa gatattaaag taagattttc agatgttgct
    11161 ggagctgaag aagaaaaaca agaactagtt gaagttgttg agttcttaaa agatccaaaa
    11221 cgattcacaa aacttggagc ccgtattcca gcaggtgttc ttttggaggg acctccgggg
    11281 acaggtaaaa ctttgcttgc taaggcagtc gctggagaag caggtgttcc attctttagt
    11341 atctcaggtt ctgactttgt agaaatgttt gtcggagttg gagctagtcg tgttcgctct
    11401 ctttttgagg atgccaaaaa agcagcacca gctatcatct ttatcgatga aattgatgct
    11461 gttggacgtc aacgtggagt cggtctcggc ggaggtaatg acgaacgtga acaaaccttg
    11521 aaccaacttt tgattgagat ggatggtttt gagggaaatg aagggattat cgtcatcgct
    11581 gcgacaaacc gttcagatgt acttgaccct gcccttttgc gtccaggacg ttttgataga
    11641 aaagtattgg ttggtcgtcc tgatgttaaa ggtcgtgaag caatcttgaa agttcacgct
    11701 aagaataagc ctttagcaga agatgttgat ttgaaattag tggctcaaca aactccaggc
    11761 tttgttggtg ctgatttaga gaatgtcttg aatgaagcag ctttagttgc tgctcgtcgc
    11821 aataaatcga taattgatgc ttcagatatt gatgaagcag aagatagagt tattgctgga
    11881 ccttctaaga aagataagac agtttcacaa aaagaacgag aattggttgc ttaccatgag
    11941 gcaggacata ccattgttgg tctagtcttg tcgaatgctc gcgttgtcca taaggttaca
    12001 attgtaccac gcggccgtgc aggcggatac atgattgcac ttcctaaaga ggatcaaatg
    12061 cttctatcta aagaagatat gaaagagcaa ttggctggct taatgggtgg acgtgtagct
    12121 gaagaaatta tctttaatgt ccaaaccaca ggagcttcaa acgactttga acaagcgaca
    12181 caaatggcac gtgcaatggt tacagagtac ggtatgagtg aaaaacttgg cccagtacaa
    12241 tatgaaggaa accatgctat gcttggtgca cagagtcctc aaaaatcaat ttcagaacaa
    12301 acagcttatg aaattgatga agaggttcgt tcattattaa atgaggcacg aaataaagct
    12361 gctgaaatta ttcagtcaaa tcgtgaaact cacaagttaa ttgcagaagc attattgaaa
    12421 tacgaaacat tggatagtac acaaattaaa gctctttacg aaacaggaaa gatgcctgaa
    12481 gcagtagaag aggaatctca tgcactatcc tatgatgaag taaagtcaaa aatgaatgac
    12541 gaaa
//
LOCUS       DAVKGC010000054        12356 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKGC010000054 DAVKGC010000000
VERSION     DAVKGC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12356
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.054"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(47..1714)
                     /locus_tag="VW049_001261"
     CDS             complement(47..1714)
                     /locus_tag="VW049_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9432386.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(1717..2082)
                     /locus_tag="VW049_001262"
     CDS             complement(1717..2082)
                     /locus_tag="VW049_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9432387.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(2239..2427)
                     /locus_tag="VW049_001263"
     CDS             complement(2239..2427)
                     /locus_tag="VW049_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9432388.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSCKVERV"
     gene            complement(2543..2719)
                     /locus_tag="VW049_001264"
     CDS             complement(2543..2719)
                     /locus_tag="VW049_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9432389.1"
                     /translation="MDLDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENVTQDLI"
     gene            complement(2830..3028)
                     /locus_tag="VW049_001265"
                     /pseudo
     CDS             complement(2830..3028)
                     /locus_tag="VW049_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
     gene            complement(3456..5000)
                     /locus_tag="VW049_001266"
     CDS             complement(3456..5000)
                     /locus_tag="VW049_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9432390.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            5213..5515
                     /gene="gatC"
                     /locus_tag="VW049_001267"
     CDS             5213..5515
                     /gene="gatC"
                     /locus_tag="VW049_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9432391.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDHLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            5515..6981
                     /gene="gatA"
                     /locus_tag="VW049_001268"
     CDS             5515..6981
                     /gene="gatA"
                     /locus_tag="VW049_001268"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9432392.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            6981..8423
                     /gene="gatB"
                     /locus_tag="VW049_001269"
     CDS             6981..8423
                     /gene="gatB"
                     /locus_tag="VW049_001269"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9432393.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            8547..9590
                     /locus_tag="VW049_001270"
     CDS             8547..9590
                     /locus_tag="VW049_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEU9432394.1"
                     /translation="MATMKAARWHAAKDVRIQEIEVPEVLPHQVKVAVKFTGICGTDL
                     HEFLDGPIFIPTDEHVYSGQKAPVTLGHEFSGEIIEVGSDVTRVKVGDRVAVEPILAK
                     NNLVGDYNLDPNLNFVGLAADGGFAKYCVLDGDLVHVIPDSLSYEQAALTEPAAVAVY
                     AVRQSSLKAGDTAVVFGLGPIGLLIVEALRAAGASKIYAVELSPERQAKAEELGAIVV
                     RPEEGETAVEAIHRLTNGGVDVSYEVTGVPVVLGQALAAVHKAGECMVVSIWEREANI
                     NPNEFAIQEKTLKGIIAYRHIFPKVLELMEQGYFSAEKLVTKKIKLENIVEEGFIELT
                     QDKSQIKILVEPE"
     gene            complement(9723..9800)
                     /locus_tag="VW049_001271"
                     /pseudo
     CDS             complement(9723..9800)
                     /locus_tag="VW049_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            9968..10528
                     /gene="efp"
                     /locus_tag="VW049_001272"
     CDS             9968..10528
                     /gene="efp"
                     /locus_tag="VW049_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9432395.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            10550..10939
                     /locus_tag="VW049_001273"
     CDS             10550..10939
                     /locus_tag="VW049_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9432396.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            10932..11354
                     /gene="nusB"
                     /locus_tag="VW049_001274"
     CDS             10932..11354
                     /gene="nusB"
                     /locus_tag="VW049_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9432397.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFVYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(11403..11630)
                     /locus_tag="VW049_001275"
     CDS             complement(11403..11630)
                     /locus_tag="VW049_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9432398.1"
                     /translation="MLSGLIFGLSHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRL
                     TILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(11652..12127)
                     /locus_tag="VW049_001276"
                     /pseudo
     CDS             complement(11652..12127)
                     /locus_tag="VW049_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12145..12330)
                     /gene="briC"
                     /locus_tag="VW049_001277"
     CDS             complement(12145..12330)
                     /gene="briC"
                     /locus_tag="VW049_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9432399.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
BASE COUNT         3667 a         2745 c         2373 g         3571 t
ORIGIN      
        1 tttactaaaa agaaaaaaga actgatttct cagtccttca ttaatcttat tccacactaa
       61 ataggtatgg gtaaacaggt tgttgacctt ggtgaatctc gacttcaacg tcttcgaatt
      121 cttctacgat ttcttgagcg atttcattgg caagttcttc gcttccgtct tcacctacat
      181 agaaggttac gatttcactg tcttcatcca acatatgttt caaggtttca gtcaatgttt
      241 ggtgcatatc agggtttgac acaagaattt ttccatccac catacctaaa ttatcgtttt
      301 catggatttc taagccatcg atcgttgtat cacgcacggc tgttgtgacg cttccgctaa
      361 cgacatcgct aagagcagct gtcatacgct cttggttttc ttcaatggac ttgcttggat
      421 caaaggcaag aagacttgtc ataccttgag gaagagtgcg agcctctacc actaccgctg
      481 gttgctccaa aacttctgcc gcagattgag ctgccatgaa gatgttcttg ttgtttggca
      541 agaagatgat gttacgggca ttaacctgtt caacagcctt gataaagtct tctgttgaag
      601 ggttcatggt ttgaccgcct tcgataacat aatccacgcc ttgagaacag aagatatctg
      661 ctagaccttt accagccacc acagcaatca aagcatactc tttttcttca gccgacttga
      721 taacttgagt agcttctttc tcaacctgtg cttcgtgttg gttacgcata ttgtcaactt
      781 ttaccttgac caagctacca tatttgagac cttcttgcat aacaagtcct ggatcttctg
      841 tatgaacatg gactttgaca atttcatcat cgttaacaac gaggagagaa tctccaagct
      901 catccaagta gttacggaat tcatcgtagt caaaatcttt agcataggtt ggaccttgct
      961 taagagctac catgatttca gtacagtaac caaacgtgat gtcctcagtc gctacgtgac
     1021 cagctacaga cttatgatgc tctacattga tcatctcact catgttggca ggagtcgcta
     1081 caaagtcctc agatgcaata tattcgccag taagggctga aaggaaacct tcgtagatga
     1141 agaccaatcc ttgaccacct gagtccacaa cgccaacttc tttcaatact ggaagcatgt
     1201 ctggtgtttt agctagagct gttttagcac cttccaaggc tgcgcgcatg acttcaacag
     1261 cgtcatctgt ttgctcagct tttttcttag caccgatagc agctccacga gaaactgtta
     1321 aaatcgttcc ttcaacaggt ttcatcactg ccttataggc aacttccaca cctgattgga
     1381 aggccagagc caagtcttga cctgttaact cgtctttatc cttgatagct tgggaaaatc
     1441 cacggaaaag ctgagacgta atcactcctg agttcccacg cgcacccatc aaaagccctt
     1501 tggcaagaat gctcgctact tctccaactg tagaagctgg cttgtctgca acttctttag
     1561 caccattttc aatggtcatt cccatatttg tcccagtatc tccatctgga actggaaaga
     1621 cgtttaatga attgacatat tcagcttgct tattcaagcg agttgatgca gcctgcacca
     1681 tttcttgaaa taagctagta gtaatttttg acacgattat tctcctacaa ctttgatatt
     1741 ttgaatgtag acatttacag tctgagcagt aattccaagc tggttttcca agctaaaggc
     1801 aacacgctct tgaatgtttt ttgacacttc actaatcttt gttccgtagc ttaacacggt
     1861 atatacatca actgcaatac tgccatcttc ggctgccttt acgacgacac ctttagaata
     1921 attttcctta cctagcaggg cttggaaatt atctttgagg gcatttttac tagccatacc
     1981 gaccacacca aaaatctcag ttgctgctcc acctacgacg gttgcaatca cttcatctgt
     2041 tagttcgatt tgaccatctt ttgtattaat ttttacagtc atccttttta cctcaactag
     2101 ttgatactct attttatcat atttcagccc aagtgtaaaa gcagaatgat gtatcagcgg
     2161 atatttactc tatttttcaa acgatgttat acccacaata aaagaaaaaa gaccctaagg
     2221 tctcctttgc ttttattatt aaacgcgttc aactttacat gatttcaaag cacgagctga
     2281 agcccaaact tttttaggtt taccatcgat aagaacagta actttttgaa ggtttggttt
     2341 tacggcacgt tttgtttggt tcatcgcgtg tgaacggttg tttcctgata cagtcttacg
     2401 acctgtaaag taacatactt tagccattgt gttttcctcc tattagatct aatatagcgg
     2461 atgtgctagc accacatacc gtactatgtt atcacacttt cttgtttttt gcaagggaat
     2521 tggaagattt tttatttgtg tcttaaatca ggtcttgcgt gacattttct gctctccaca
     2581 tgccattgtt gattaacaga acaccagaat taaaattatg tgtataaaaa tcatctctaa
     2641 ctgcagctaa gggtatagcc gtcaagtcca aatcccacag ctcatctatc gattttctta
     2701 caacaatatc taaatccaaa tacagtacac gagactcgct tacatacttt ggaataaaat
     2761 atctaaaaaa gccgcatatg aaagtccctc aaaggggaga cgataacctt tcagaatact
     2821 actgtcaatc taaacattca caatttcact attcaaagtc tctagtcttt tttccatcaa
     2881 ttggaaccat tctcgcggaa ggtcatcatt aaaaacataa aacttaagat tataatagat
     2941 gaacacaaag agattttatt gttgtttcaa ctttatccat ataagcatta tctgcaccta
     3001 agacaatcgc ttttttctct tctttcactt tttatctcat ttctttttat tcccatcata
     3061 tgtttctacg taaccattat tttcgcctat tcgttcgtaa aaccatacca gtggagattt
     3121 tagatgaagt cccattacgg tttacaattt ttacattacg acacggagtt ttacaaatcg
     3181 atttcatttg ccaaacgtag ttagtaaggc agttagctag gctacctact ttctgctacg
     3241 acggaaaaat ccacaaatca ataccattcg tggatttttc ctagtgaagc gtttaggtaa
     3301 gccgccatag cggttaccga ttattagatt acgacacgga gtttggcaaa tcgattctat
     3361 ttgccaaacg tagttagtga ggcagttagc tagttcgcca aatagcgact agcgtccaac
     3421 aatttggaac tttagttcca attgttggta ctgagtcaca tcttctcctc taactctacg
     3481 tctggatact tgtccgcaaa ccagcggagg gcaaagtcat tttcaaagag aaagactggt
     3541 tggtcaaaac ggtctttggc taagatattg cgacttgacg acatccgttc atccaagtcc
     3601 tcaggcttga tccaacgaac ggtcttttta cccattgggc tcatgaccac ttccgcattg
     3661 tattcgcctt ccatacggtg tttaaagact tcaaactgga gttgaccaac agctcccagc
     3721 atgtactcac ctgtttggta attcttataa agctgaacgg ctccttcttg caccaattgc
     3781 tcaatcccct tgtggaagga tttttgcttc ataacattct tagcagaaac tttcatgaaa
     3841 atttcaggag taaaggttgg cagtggttca aattcaaact tgttttttcc aaccgtcaag
     3901 gtatccccaa cctgataagt accggtatcg taaaccccga taatatcacc tgccacggca
     3961 ttgatcacat tctcacgact ttccgccata aactgggtaa cattagatag tttagccccc
     4021 ttaccagtac gagggagatt gacactcatg ccgcgctcaa attcgcctga tacgatacgg
     4081 acaaaggcaa tacggtcacg gtgacgagga tccatgttgg cttggatttt aaagacaaag
     4141 cctgagaaat ccttgtcata aggatccaca atttcaccgt ctgttttctt gtgaccatgt
     4201 ggttctggag caaacttgag gaagatttca aggaaggtct gcacaccaaa gtttgtcagg
     4261 gctgaaccga aaaagacagg cgtcaattct ccagccagaa tagcttcctc tgaaaactca
     4321 ttcccagctt catttaaaag ctcaatgtca tccttgactt gctcgtagaa aggattgcta
     4381 ccaaagagtt tgtccccgtc ttctagactg gcaaaacgct catccccttt gtaaagctct
     4441 aaacgttggt tatagaggtc atacaagccc tcaaaggctt tccccatccc gataggccag
     4501 ttcatagggt agctagcaat gcccaagatt tcttccaatt cttgcaagag atccaaaggc
     4561 tcacgaccgt cacggtccag cttgttcata aaggtaaaaa ctggaatgcc acgatgtttc
     4621 acaacctcaa acaatttctt ggtttgagcc tcgatcccct tggcagagtc cacgaccatg
     4681 accgcagcat ccaccgccat caaggtacga taggtatctt ctgagaagtc ctcgtgccct
     4741 ggcgtgtcta agatattcac gcgcttgccg tcgtagtcaa attgcataac agatgaagta
     4801 acagaaatcc cacgttgctt ctcgatatcc atccagtcag atttagcaaa agtccctgtt
     4861 ttcttccctt ttaccgtacc agcctcacga atctcacccc caaagtagag taactgctca
     4921 gtgatggttg ttttccccgc gtccgggtgg gagataatgg caaaggtacg acgtttctta
     4981 atttcttctt gaatattcat aagttctctt tctttgattc tctatttttc ttgtttcaat
     5041 agctgagaat gatttttaca ttggatttta ccattccttt caacactcca ttatatcaga
     5101 ttttagcatt tttttcaatt tctatttctt ttcacttccc cctcccttat ttataggaaa
     5161 atatggtaaa atagaacaga ctaaaaatca tcatttcacg aaaggatgca agatgaaaat
     5221 tacgcaagaa gaggtaacac acgttgccaa tctttcaaaa ttaagattct ctgaagaaga
     5281 aactgctgcc tttgcgacca ccttgtctaa gattgttgac atggttgaat tgctgggcga
     5341 agttgacaca actggtgtcg cacctactac gaccatggct gaccgcaaga ctgtactccg
     5401 ccctgatgtg gccgaagaag gaacagaccg tgatcacttg tttaaaaacg tacctgaaaa
     5461 agacaactac tatatcaagg tgccagctat cctagacgat ggaggagatg cctaatgact
     5521 tttaacaata aaactattga agagttgcac aatctccttg tctctaagga aatttctgca
     5581 acagaattga cccaagcaac acttgaaaat atcaagtctc gtgaggaagc cctcaattca
     5641 tttgtcacca tcgctgagga gcaagctctt gttcaagcta aagccattga tgaagctgga
     5701 attgatgctg acaatgtcct ttcaggaatt ccacttgctg ttaaggataa catctctaca
     5761 gacggtattc tcacaactgc tgcctcaaaa atgctctaca actatgagcc aatctttgat
     5821 gcgacagctg ttgccaatgc aaaaaccaag ggcatgattg tcgttggaaa gaccaacatg
     5881 gacgaatttg ctatgggtgg ttcaggtgaa acttcacact acggagcaac taaaaacgct
     5941 tgggaccaca gcaaggttcc tggtgggtca tcaagtggtt ctgccgcagc tgtagcctca
     6001 ggacaagttc gcttgtcact tggttctgat actggtggtt ccatccgcca acctgctgcc
     6061 ttcaacggaa tcgttggtct caaaccaacc tacggaacag tttcacgttt cggtctcatt
     6121 gcctttggta gctcattaga ccagattgga ccttttgctc ctactgttaa ggaaaatgcc
     6181 ctcttgctca acgctattgc cagcgaagat gctaaagact ctacttctgc tcctgtccgc
     6241 atcgccgact ttacttcaaa aatcggccaa gacatcaagg gtatgaaaat cgctttgcct
     6301 aaggaatacc tcggtgaagg aattaaccca gaggttaagg aaaccattct aaatgccgct
     6361 aaacactttg aaaaattggg tgctattgtc gaagaagtca gccttcctca ctctaaatac
     6421 ggagttgccg tatactacat catcgcttca tcagaagctt catcaaactt gcaacgcttc
     6481 gacggtatcc gttacggcta tcgcgcagaa gatgcaacca accttgatga aatctatgta
     6541 aacagccgaa gccaaggttt tggtgaagag gtaaaacgtc gtatcatgct gggtactttc
     6601 agtctttcat caggttacta tgatgcctac tacaaaaagg ctggtcaagt ccgtaccctc
     6661 atcattcaag atttcgaaaa agtcttcgcg gattacgatt tgattttggg tccaactgct
     6721 ccaagtgttg cctatgactt ggactcactc aaccatgacc cagttgccat gtacttggct
     6781 gacctcttga ccatacctgt aaacttagca ggtctccctg gaatttcgat tcctgctgga
     6841 ttctctcaag gtctacctgt cggtctgcaa ttgattggtc ccaagtactc tgaggaaacc
     6901 atttaccaag ctgctgctgc ttttgaagca acaacagact accacaaaca acaacccgtg
     6961 atttttggag gtgacaacta atgaactttg aaacagtcat cggacttgaa gtccacgtag
     7021 agctcaacac caattcaaaa atcttctcac ctacttctgc ccactttgga aatgaccaaa
     7081 atgccaacac taacgtgatt gactggtctt tcccaggagt tctaccagtt ctcaataaag
     7141 gggttgttga tgccggtatc aaggctgctc ttgccctcaa catggacatc cacaaaaaga
     7201 tgcactttga ccgcaagaac tacttctatc ctgataaccc caaagcctac caaatttctc
     7261 agtttgatga gccaatcggt tataacggct ggattgaagt cgagctagaa gacggtacga
     7321 ccaagaaaat cggtatcgaa cgtgcccacc tagaggaaga cgctggtaaa aacacccatg
     7381 gtacagatgg ctactcttat gttgacctca accgccaagg ggttcccttg attgagattg
     7441 tatctgaggc agatatgcgt tctcctgaag aagcctatgc ttatctgaca gccctcaagg
     7501 aagttatcca gtacgctggc atttctgacg ttaagatgga ggaaggttcg atgcgtgtgg
     7561 atgccaacat ctcccttcgt ccttatggtc aagagaaatt cggtaccaag actgaattga
     7621 agaacctcaa ctccttctca aacgttcgca aggggcttga atacgaagtc caacgccagg
     7681 ctgaaattct tcgctcaggt ggtcaaatcc gccaagaaac acgccgttac gatgaagcga
     7741 ataaagcaac catcctcatg cgtgtcaagg aaggggctgc tgactaccgc tacttcccag
     7801 aaccagacct acccctcttt gaaatttctg acgagtggat tgaggaaatg cggactgagt
     7861 tgccagagtt tccaaaagaa cgtcgtgcgc gttatgtatc tgaccttggt ttatcagact
     7921 acgatgctag tcagttgact gctaataaag tcacttctga cttctttgaa aaagctgttg
     7981 ccctaggtgg tgatgccaaa caagtctcta actggctcca aggggaagtc gctcagttct
     8041 tgaatgctga aggtaaaaca ctggaacaaa tcgaattgac accagaaaac ttggttgaaa
     8101 tgattaccat catcgaagac ggtactattt catctaagat tgccaagaaa gtctttgtcc
     8161 atctagctaa aaatggcggt ggcgcgcgtg aatacgtgga aaaagcaggt atggttcaaa
     8221 tttcagatcc agctatcttg atcccaatca tccaccaagt ctttgccgat aacgaagctg
     8281 ctgttgccga cttcaagtca ggcaaacgta atgcagacaa ggccttcaca ggattcctta
     8341 tgaaggcaac caaaggccaa gccaacccac aagttgccct taaactactt gcacaggaat
     8401 tggcgaagtt gaaagaaaac tagacagaac aaaaccagcc ctaaggttgg ttttttcttg
     8461 acaaatagaa agaaaacgtt tgcaatattg cgtgtaaagt ttccaaacaa gaaaactaaa
     8521 aatcgcaaca ataaggagtt atttatatgg ctacaatgaa ggcagctcgc tggcatgcag
     8581 cgaaagatgt tcgtattcaa gaaatagaag ttcctgaagt acttccgcac caagtaaaag
     8641 ttgcagttaa gttcacagga atttgtggta ctgacctcca tgaattttta gatggaccta
     8701 tctttatccc aacagatgag cacgtctatt ctggtcaaaa agcaccggta acactgggac
     8761 atgaattttc tggtgaaatc atagaagtcg gaagcgatgt tacccgtgtt aaagttggtg
     8821 atcgtgttgc tgtggaacca attctagcta aaaataactt agttggtgat tataatttgg
     8881 atccaaacct taattttgtc ggtttggctg cagacggtgg atttgccaaa tattgtgttt
     8941 tagacggtga cttagtacat gtcattccag atagtttgag ctatgagcaa gctgctctta
     9001 ctgaacccgc tgctgttgca gtctatgctg ttcgtcaatc ttctttgaag gctggcgata
     9061 cagctgttgt ctttggttta ggcccgattg gtctattaat tgtagaagct cttcgtgcag
     9121 ctggtgcttc taagatttat gctgttgaac tatcacctga acgtcaagca aaagctgaag
     9181 agttaggagc tatcgttgtc cgtccggaag aaggtgaaac agctgttgaa gctattcatc
     9241 gtttaacaaa cggtggtgta gatgtttctt atgaagtaac gggtgtgcca gtagttttag
     9301 gacaagcctt agctgccgta cataaagcag gtgagtgcat ggtggtctct atctgggaac
     9361 gagaagcaaa tattaatccg aatgaatttg caatccaaga aaagacacta aaaggaatca
     9421 ttgcttatcg tcatatcttc cctaaagtat tagaattgat ggaacaaggc tacttctctg
     9481 ctgaaaaatt agttactaag aaaattaaat tggaaaatat cgtcgaagaa ggctttatcg
     9541 aattaactca agataagtca caaattaaaa ttttggtaga accagaataa aaaatcaatc
     9601 tttttaagac aatactaaca ggtcggagta aacttacttc ggccttttga aatataatac
     9661 tcttcgaaaa tctcttcaga ccacgtcagc gtcacattac cgtatatatg gttactgact
     9721 tcgtcagttc tatccacaac ctcaaaacag tgttttgagc agcttgcggc tagcttccta
     9781 gtttgctctt tgatttccat tgagtatgag aagaaatata gtggatactc tttaacatta
     9841 accactccac ctgactagcc aatattcttt ttcgctccta ccaactccca ataactattt
     9901 tggctttatt tccagagtat tttatggtaa aatgaagagt aataatattt attaaagagg
     9961 taaaaacatg attgaagcaa gtaaattaaa agctggtatg acctttgaaa cagctgacgg
    10021 caaattgatt cgcgttttgg aagctagtca ccacaaacca ggtaaaggaa acacgatcat
    10081 gcgtatgaaa ttgcgtgatg tccgtactgg ttctacattt gacacaagct accgtccaga
    10141 ggaaaaattt gaacaagcta ttatcgagac tgtcccagct caatacttgt acaaaatgga
    10201 tgacacagca tacttcatga atacagaaac ttacgaccaa tacgaaatcc ctgtagtcaa
    10261 tgttgaaaac gaattacttt acatccttga aaactctgat gtgaaaatcc aattctacgg
    10321 aactgaagtg atcggtgtca ccgttcctac tactgttgag ttgacagttg ctgaaactca
    10381 accatctatc aaaggtgcta ctgttacagg ttctggtaaa ccagcaacga tggaaactgg
    10441 acttgtcgta aacgttccag acttcatcga agcaggacaa aaactcgtta tcaacactgc
    10501 agaaggaact tacgtttctc gtgcctaatc tctagaaaga ggtcattcta tgggaattga
    10561 agaacaactt ggcgaaatcg ttatcgcccc acgtgtactt gaaaaaatca ttgctatcgc
    10621 tactgcaaag gtagagggtg ttcactcttt ttcaaacaga tcagtgtctg ataccctttc
    10681 aaaactttca ctcggccgtg gcatttatct taaaaacgtg gacgaagaac tcacagcaga
    10741 tatctatctc taccttgagt acggagtaaa agttcctaag gtagcggttg ctatccagaa
    10801 agctgtcaaa gatgccgtcc gtaatatggc tgatgtagaa ctcgctgcta tcaatattca
    10861 cgttgcaggt atcgtcccag ataaaacacc aaaaccagaa ttgaaagatc tatttgacga
    10921 ggacttcctc aatgactagt ccactattag aatctagacg ccaactccgt aaatgcgctt
    10981 ttcaagctct catgagcctt gagttcggta cggatgtcga aactgcttgt cgtttcgtct
    11041 atactcatga tcgtgaagat acggatgtac aacttccagc ctttttgata gacctcgttt
    11101 ctggtgttca agctaaaaag gaagaactag ataagcaaat cactcagcat ttaaaagcag
    11161 gttggaccat tgaacgctta acgctcgtgg agagaaacct ccttcgcttg ggagtctttg
    11221 aaatcacttc atttgacact cctcagctgg ttgctgttaa tgaagctatc gagcttgcaa
    11281 aggacttctc cgatcaaaaa tctgcccgtt ttatcaatgg actgctcagc cagtttgtaa
    11341 cagaagaaca ataaaaagaa aaagtgtttg atatctatca aacacttttt ctttgcctcc
    11401 cactatctaa aaaaatgata atagatataa ttgtaaacaa aaatccagat aggttttgca
    11461 tgattgagaa agttaaaaaa actatggcag agaatcgtta atctcagatt gtcggtagaa
    11521 cgataaacaa gggcaaaaaa gaaaccaatc agactataat ataataaact aattggatct
    11581 ctgtgagata gtatcaaatg actaagccca aagataaggc ctgataagat gacatctaaa
    11641 taatacttgg actagggaaa gaaggtattc ataaaatacc ctctatcaag agtctcctca
    11701 aaaacaggac cgatgattac aggcaggaca aaagataaga tagtcgataa aaaggttggt
    11761 tgtccatttg aaagaaccac ggtaaaatac tcatcatgaa tattcctatg attaatcaaa
    11821 tgagcatagc gtgcccaaaa attaccgaga atctgataaa ccacataagt tgcaaataag
    11881 gagaagacaa atgaccagtt ccagctcttt ttctcaaaga taaagagcat ctttttcttt
    11941 tttaacctcc aaattaatag aaaaggaaac ttcccactaa tcccactgtt aaaataagag
    12001 aatagacatc agctcctaac cctaaaatga tcgtcacata caatccaatt gtttgtggta
    12061 aataggtaga tagtaaaata ataagcaaaa atattccaaa ttgtcttagt ttctttgtgt
    12121 ttctcatcgt acttttttga aagattaccc tgttcggaag ccgtacttcc aagcatctat
    12181 ataagaatta agtgcccctt gcctcatata aggagcaaat tctctataat ataaccatct
    12241 actatatcca tcttcccaaa cagcaagacc acctgaagtt tgctccaagt cctcagttga
    12301 aagaactgta aatgtatttg tacctgtcat tgcaagtacc ttcttaaaat atagtg
//
LOCUS       DAVKGC010000055        12178 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKGC010000055 DAVKGC010000000
VERSION     DAVKGC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.055"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            165..458
                     /locus_tag="VW049_001278"
                     /pseudo
     CDS             165..458
                     /locus_tag="VW049_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            565..916
                     /locus_tag="VW049_001279"
                     /pseudo
     CDS             565..916
                     /locus_tag="VW049_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1192..2498
                     /locus_tag="VW049_001280"
                     /pseudo
     CDS             1192..2498
                     /locus_tag="VW049_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2538..3548)
                     /gene="tsaD"
                     /locus_tag="VW049_001281"
     CDS             complement(2538..3548)
                     /gene="tsaD"
                     /locus_tag="VW049_001281"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9432400.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3538..3975)
                     /gene="rimI"
                     /locus_tag="VW049_001282"
     CDS             complement(3538..3975)
                     /gene="rimI"
                     /locus_tag="VW049_001282"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9432401.1"
                     /translation="MIEIKRIQQQPDLAKAIYAVMIAVYPVSPWTLEQIQADLFQDQT
                     WYALAYDGAEVIGFLAVQETLFEAEVLQIAVKGAYQGKGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIADRKAYYHDPVEDAIIMKREIDEG"
     gene            complement(3972..4655)
                     /gene="tsaB"
                     /locus_tag="VW049_001283"
     CDS             complement(3972..4655)
                     /gene="tsaB"
                     /locus_tag="VW049_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9432402.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPR
                     QKEGLVVPIMDARRNNVYAGFYENAKPVLPEAHLSFAEVLEQVKDAEQVTFVGEVGAF
                     VEQIQEQLPQASYQETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            4833..5018
                     /gene="cibA"
                     /locus_tag="VW049_001284"
     CDS             4833..5018
                     /gene="cibA"
                     /locus_tag="VW049_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9432403.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5020..5172
                     /gene="cibB"
                     /locus_tag="VW049_001285"
     CDS             5020..5172
                     /gene="cibB"
                     /locus_tag="VW049_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9432404.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            5990..6223
                     /locus_tag="VW049_001286"
     CDS             5990..6223
                     /locus_tag="VW049_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9432405.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6225..7904
                     /locus_tag="VW049_001287"
     CDS             6225..7904
                     /locus_tag="VW049_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9432406.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8005..8094)
                     /locus_tag="VW049_001288"
                     /pseudo
     CDS             complement(8005..8094)
                     /locus_tag="VW049_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8283..10196)
                     /gene="mnmG"
                     /locus_tag="VW049_001289"
     CDS             complement(8283..10196)
                     /gene="mnmG"
                     /locus_tag="VW049_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9432407.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNYFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10206..10661)
                     /locus_tag="VW049_001290"
     CDS             complement(10206..10661)
                     /locus_tag="VW049_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9432408.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKSQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWKGQLRHIHLEE"
     gene            complement(10802..11923)
                     /gene="mnmA"
                     /locus_tag="VW049_001291"
     CDS             complement(10802..11923)
                     /gene="mnmA"
                     /locus_tag="VW049_001291"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9432409.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3417 a         2879 c         2450 g         3432 t
ORIGIN      
        1 atcatgctta tatctcaaag atacctattt tctcttgtct cgaccttctc caaagaattg
       61 ctataatact attacaaata catctgcact acacttcaaa ttttagcact gtataaaaac
      121 gtttcaacac actaacttta agaaaacttc cactattaat tgaaaaagtt gatagagata
      181 aattaaagat ctatattgaa actcatcccg atgcttattt gactgaaata gctgctgaat
      241 tcaactgtcc tccaacaact attcattacg ctctaaaggc tatgggatat agtctaaaaa
      301 agagctgtac ctactgcgaa caagacccag aaaaagtaaa tcggttcctt aaagaattga
      361 atcacttaag ctacctgact cctatttata tttatgagac aggggttgag acctattttt
      421 atctcgaata tgatcgagcc ttgagcaggc agttagtctc tctggaagaa gatataatta
      481 tttgaattaa gatcgagaca acgcacacca gagattgcga tactgttata gaagtactaa
      541 tgcccttttt tgtttcaata tactatggct ccgatgacct ataaagatac gatgacgagt
      601 gactttttcg aagcttgctt ccaaaaattc ttactaccta ctttagatac accatccctt
      661 atcattatgg acaatgcaag gtttcacaga atgaacatgt gtaaggagca gggcatagac
      721 tgttaccact tcctacctat tcacccgagt ataatcccat tgagaaaata tgggcttaca
      781 tcaaaaacat ctcagaataa tattgtcaaa ttacgatgct tttcttgagg cacttttgtc
      841 ctattcttgt ttcagccgac tatactccgt tattgggcag ctacggaaca gtcgatggga
      901 cgatgggggg acataaaaaa atcctccagt tttgtttttt ataacagtat actggagaat
      961 tgacaatctc ggtagatacc tcgttatagc gcggttactt attaggcagt tacaaaacaa
     1021 ctgtgaacag aaaacattcc agagtcagac aagactttgg aatgttttgg ctctataatt
     1081 tctgtagtgg gtaatcccac cccaggaatt atagggtcgt ttcttgtaga aaaaaagccc
     1141 catatgacct ataatgaaaa gcgtctaacc aactcattag aaagggttca tatggaacaa
     1201 cttaagaata ccacagattt gctcggattg aaagacaaaa atatcaaaat cttgtctgtt
     1261 ctgaaatacc aaacccatct agtcgttcag gcaaagttgg attcccccgc tcctccttgt
     1321 cctcattgtc aagggaagat gatcaaatac gacttccaga aagcctctaa aattccgctt
     1381 ctcgactgtc agggtttacc cacggtacta catctcaaaa agcgccgctt tcagtgcaag
     1441 aattgcctta aggtggtcgt ttctcaaaca tccattgtca agaaaaattg ccagatttcc
     1501 aacatggtga gacaaaaaat cgctcagctc ctccttgaaa agcagtctat gactgagatt
     1561 gcccacagat tggcggtctc aacttccacc gtcatccgaa aactgaggga atttaagttt
     1621 gaaaccgatt ggaccaagtt gccaaaagtt atgagttggg atgagtatag cttcaaaaag
     1681 agcaaaatga gcttcattgc ccaagatttt gagtccaaat ccatcctcgc aattttagac
     1741 gggcgaactc atgcggtgat tcgaaaccat ttccaacgct atcagagaga ggttcgggag
     1801 ctggtcgagg tcatcaccat ggacatgtac agcccttatt atcggctcgc taagcaactc
     1861 tttccaaagg cgaagattgt tcttgaccgc ttccacattg tccaacatct gagccgagct
     1921 atgaaccgag tacgaatcca aatcatgaac caatttgacc gaaaatcctt ggagtatcgg
     1981 gcgctcaagc gcttttggaa ccctcgcttt ttcgtttcta ggctcgggct aaatcagtcc
     2041 actggactga tttactacac caggatagct tcaagctctg tcagaaacga ttctatcagc
     2101 ccacgtttcg aatgcactta acccatcggg aagtacgaga taagctgctt tcttactctg
     2161 agggattaca ggttcactac gaactctatc aactcctgct ctttcatttt caagagaaga
     2221 atgccgacca tttctttgga ttgattgagc aagaactgcc aacggttcat ccgctttttc
     2281 aaacggtctt ttggactttt ttaagggata gagataagat tatcaacgca cttaagctgc
     2341 cttattccaa cgctaaactt gaagcgacca ataatttgat taaggttatc aagcgcaaag
     2401 cctttggttt ccggaacttt aacaatttta aaaaacggat tttgatgact ttgaacatca
     2461 aaaaagagag tacgaatttc gtactctcca gattgtagct tttcgcctac ccactacact
     2521 tgacaaagag ccactcttta ttccatggta tcaaaggcaa gacttggttt ggcattgagg
     2581 tcccagcctg cgaagttttc tttgttccac tcgctgacgc tggcataggc aatcatacct
     2641 gcattgtctc cgcagagtcg cagagggggg atgataacct tgacatctgt gatttcggct
     2701 gctaggcgtt ctctgagacc tttattggct gccacaccac ctgccacaac tagggtttta
     2761 acaggatatt tctccaaagc cttcttggtt tttgccatga gaatgtccat aactgctgct
     2821 tggaaggaag cacacaaatc ttctgtagac aggctttctc ccttttgctc ggcattgtga
     2881 tgaagattga taaaggcaga tttcaaacct gagaaggaga actccagatt atcttcctta
     2941 atcatggcac gggggaaatc ataaatatcc tgcccctgat gagccagctc gtcaatctca
     3001 cgacctgcag gataggtcaa gcccatgaca cgaccgacct tgtcataagc ctccccaact
     3061 gcatcgtctc gtgtctcccc aacaatcttg taatctcctg cctccgaaac atagaccaac
     3121 tctgtgtgcc caccgctaac caagagggct agcaagggaa actccaaagg ctccacactc
     3181 tgagctgcca tgaggtgccc agccatgtga ttaacaggaa tcagtggaag tccgtgagcc
     3241 caagcaaagg ccttggcagc tgacaaacca actagcaagg ctccgaccaa gcctggtccg
     3301 taggtaaccg caacagctgt cacatcctct tcggtaatcc ctgcttctgc caatgcctcc
     3361 tcgatacagg ctgtaatgac ctcgacatgg tgacgactgg ctacttcggg cactacgcca
     3421 ccaaaacgtt tgtgactctc aatttgacta gcaatgacat tggacaagag ctcatcgtcg
     3481 tttttcaaga cggcgacact ggtctcatca caggatgtct caaatgctaa aatatatcta
     3541 tccttcatct atttctctct tcatgataat ggcgtcctcg actgggtcat ggtagtaggc
     3601 ctttcgatca gcgatgaccg ccattttttc tttcttgtaa aatgcttgcg ctcgttgatt
     3661 tgactttctg acttcgagga aaatctcctt atctgtcggc aattgagcaa acaaggctga
     3721 cgcaatgccc ttaccctgat aggctccttt gacagcgatt tgcaggactt ctgcttcaaa
     3781 gagagtctcc tgaacagcta gaaagccaat cacttctgcc ccatcataag ccagagcata
     3841 ccaagtctgg tcttggaaca ggtcagcttg gatttgttcc agcgtccaag gactgactgg
     3901 gtaaacagct atcataacag cgtagatggc tttagctaga tcaggctgct gttggattcg
     3961 tttgatttct atcataggcg tttaatgtaa gactcgccag actcggtatg gttcttgagc
     4021 cagttttcct cagcctcgac acgcttgagg taatttggca caaagtcgtg caaggagtct
     4081 gcttctttgt cccaagccca aagagctaga ttagctgcat ttggcaaggt ttcttggtag
     4141 ctggcttgtg gtagttgttc ttggatctgt tccacaaagg ctccaacttc tccgacaaag
     4201 gtcacctgct cagcatcctt gacctgctct agcacttccg caaaagacag atgcgcttct
     4261 ggcaagactg gcttggcatt ttcataaaag cctgcataga cattgttgcg acgcgcatcc
     4321 atgataggaa ccaccaagcc ttctttttgg cgtggcacca gagccaatag actagacatc
     4381 ccaactaact caatgttcag ggtatgagcc aaggtcttgg cagttgctac cgcaattcgc
     4441 aagcctgtat agctacccgg cccttcagct accacgattc ggtccaaatc cttgggtgtc
     4501 caatccaaac ttgccatcaa aaaatctatg gcaggcataa gagtaatact gtgatttttc
     4561 ttaatattaa tcgtcgtctc ggcaagaacc tgcttgtcct ctaaaatagc gagagaaaga
     4621 gccttgctgg acgtatcaaa agctaatact ttcataacac attcctatct ttttgtctgc
     4681 ttactattat actacaaaag ctggcacatg ggaattttct ttgcccccag acaagagtgc
     4741 cctcacttaa ctaaaaataa tttaaaaaaa tgctcacttt tccttttctt ttccgaatat
     4801 aaaagtgaac aagaaaaaag gaggaaagtt caatgacaaa ttttgacatt cttgacaatc
     4861 aatttttatc cttatctgaa aatgaattat cagatattga tggcggtctc gctcccttgg
     4921 ttatctttgg agtagcagta tcttggaagg ctattgcagg tggaacagca cttataggtt
     4981 ctggtttggc agctggttat tttttaggag gagattaata tgatgaaaaa tttgaacaac
     5041 tatcgtgaaa tttctaataa ggaattgcaa gaaatcaagg gtggctttgg tgtaggtgtt
     5101 ggtatcgctt tatttatggc aggttatacc attggaaaag accttcgtaa aaagtttggt
     5161 aagtcatgct agataagaaa cacattttta gaaggataaa ttttattgtc ttcatctctt
     5221 acagtttgct cagcattctc aatgatttga acattactac tatcccttta ccattcgatt
     5281 tatctgtttg tattgtttta tttttatgct tcaactctat ttttgagcag aacaatgact
     5341 cccataaaaa taataagctt tgaaaattcc attgtcatgt cattttagaa aaatgcaaag
     5401 accacctcat cttgatagat ggggtggaat tttcgtgtcg taaatctact atctctacat
     5461 ccccaaacaa aaaaccccag cataagcagg gcatctaagc atttaatcca aagtaaaata
     5521 caaaccaaac gacatagggc acgaggagga gaaaaagcga gtagagagtc acaaaggtaa
     5581 ttttccacaa gaacttggtt tgtcatcgtt ccagtttggc aaatggaaga tttcctccat
     5641 aaacgcaagc aacaaaaacg ataaaagcta acaaacgaac tccgatagca aaagcaaata
     5701 agttatacat agggcaacct ccttgactta aaatctatat ggaattatga caagcaataa
     5761 atttcacttc cgttatcaac ataatacatt ttctttattt ttgtaaacgc ttaccaaaga
     5821 aattgccccc taactttctc gtttccgtct tttactaatt tttcattttg tggtataatt
     5881 gaaataattg taacgaatca aggtcaatct agacacaaaa tggaatgaaa tcaagcaaat
     5941 atctgctaaa agtttggaat aagctgacct gtaaatagaa aggaactata tgatttacaa
     6001 agttttttat caagaaacaa aagaacgtag cccacgccgt gaaacaacac gcgcgcttta
     6061 cctagacatc gatgccagct cagaacttga gggccgtatc actgctcgcc aacttgtcga
     6121 agaaaatcgc ccagagtaca atatcgaata tatcgaactc ttgtctgaca aattgctcga
     6181 ttacgaaaaa gaaactggcg ccttcgaaat tacggagttc taatatggcc tacactctta
     6241 aacctgaaga agtcggcgtt tttgccatcg gtggtctagg agaaatcggg aaaaacactt
     6301 acggaattga ataccaagac gagattatca tcgtcgatgc tgggattaaa ttcccagaag
     6361 atgacttgct tggtatcgac tatgtcattc ctgactactc ttacatcgtg gacaatatcg
     6421 accgcgtcaa ggctgtttta atcacacacg gacacgagga ccacattggt gggattccgt
     6481 tcctactcaa gcaagcaaat gtccctatct acgctggacc gcttgccttg gctttgatcc
     6541 gtgggaaact cgaagaacac ggcctcttgc gcaacgccaa actttacgaa atcaaccaca
     6601 acaccgagtt gacctttaaa aatctcaagg caactttctt tagaacgact cactctattc
     6661 cagagccttt ggggattgtc attcatactc ctcaagggaa aatcgtctgt acgggggact
     6721 ttaagttcga ctttactcca gttggagaac ctgcggactt gcatcgtatg gctgcgcttg
     6781 gtgaagaagg tgtgctctgt ctcctgtctg actcgacaaa tgcggaagta ccaaccttta
     6841 ccaactctga aaaagtcgtt ggtcagtcca ttatgaagat tatccaaggt attgaaggac
     6901 gtatcatctt tgcatccttt gcctcaaata tcttccgtct ccagcaggca acagaagctg
     6961 ctgttaagac tggacgcaag attgcggtct ttggtcgttc tatggaaaag gccattgtca
     7021 acggaatcga tcttggctac atcaaagctc ctaagggaac ctttatcgag ccaaatgaaa
     7081 tcaaagatta tcctgcagga gaagttctta tcctctgtac aggtagtcag ggtgagccta
     7141 tggcagccct ctctcgtatc gccaacggaa cccaccgtca agtacaatta caaccaggtg
     7201 ataccgttat cttctcttct agtcccatcc ctggaaacac tactagcgtc aacaagctga
     7261 ttaacatcat ttctgaagct ggtgtcgaag ttatccacgg taaagtgaac aatatccata
     7321 catctggaca cggtggtcag caagagcaaa aactcatgct ctgcttgatt aagccaaaat
     7381 acttcatgcc tgtccacggt gaataccgca tgcaaaaagt ccacgctgga ctagcagtgg
     7441 atactggtgt tgagaaggac aatatcttta tcatgagcaa tggcgatgtg cttgccctta
     7501 ctgctgactc agctcgtatc gcaggtcatt tcaacgccca agatatctat gtcgatggaa
     7561 atcgtatcgg tgaaattggc gcagctgtcc tcaaagatcg tcgcgatcta tctgaagacg
     7621 gtgtcgttct ggcagtcgca actgttgact tcaaatcgca gatgattcta tctggtccag
     7681 acatcctcag ccgaggcttt gtctacatga gagagtctgg cgacttgatt cgccaaagcc
     7741 agcgtatcct cttcaatgcc attcgtatcg cactgaaaaa taaggatgct agcgtgcaat
     7801 ctgtcaatgg tgccattgtc aacgctattc gccccttcct ctatgaaaat accgaacgtg
     7861 aaccgatcat catcccgatg atcctcacac cagatgaaga ataatactct tcaaaccacg
     7921 tcagcttcgc cttgccgtat atatgttact gacttcgtca gttctatcca caacctcaaa
     7981 acggtgtttt gagctgactt cgtcagttct atctacaacc tcaaagcagt gctttgagca
     8041 acctacggct agcttcctag tttgctcttt gattttcatt gagtacaagt caacaaaaca
     8101 gccccgtctt cggagctgtt tttctctatg ctttcttttg agattaaaac tcaatgaaaa
     8161 tcagaccaga aggctaggaa aatgcataac ttgagttagc tcccatagtt cgggaaacta
     8221 tgggaggctg gagatgaatc aaagccaagc tttgaactca ttcgtaagaa gccgacgacg
     8281 tatcattttg atttttgaag agttttagaa atactacgat ttttaccttc cagatacacc
     8341 atcaaaatag aaatatctgc tgggtttacc cccgaaatac ggctggcttg gccgatggtt
     8401 tctggattga tgagtttgaa cttctgacga gcttccgtcg caatagaatc gatgtcatcc
     8461 cagtcgatat tggctggaat gcgtttttct tccatacgtt tcatcttggc aacctgatcc
     8521 atggctttgg aaatatagcc ttcgtacttg atttctgttt caatcaattc gataatcttg
     8581 tcatccaagt cttctgcagc tggtccgatg aaggccacca catcttggta agaaacttct
     8641 ggacggcgaa ggaattcctt ggctgtcact gcatcggtca acggcttgaa gcccatttcc
     8701 tcaaccttag cattggtttc cttgactggc ttgagtttga tactgtctag acgcttcatc
     8761 tcattatcaa attgattttt cttgatttca aaacgagccc agcgttcatc gtccacaagg
     8821 ccaatctcac ggcccatctc agtcaagcgc atatcagcat tgtcatggcg aagaatgaga
     8881 cgatattcag cacgactggt caagagacgg taaggttcaa tggttccctt ggtcaccaag
     8941 tcgtcgatca tcaccccgat ataaccgtca cttcgtttta gaatcaattc aggcttgcct
     9001 tggattttca gagccgcatt gatacccgcg ataatccctt ggcctgctgc ctcttcgtaa
     9061 cctgacgttc catttgtctg accagcagtg aagagacctg agattttctt ggtttccaaa
     9121 gtcgcacgca actgatgagg caagaccata tcatactcaa tagcataacc tgtccgcatc
     9181 atctctgcat tttccaaacc tttgatggaa tgcaccaagt cacgctggac atcctcaggc
     9241 agactggttg aaagtccttg cacatagact tcctcagtat tgcgcccttc tggctcaagg
     9301 aagagttggt gacgttcctt gtccgcaaag cgcacaatct tgtcttcaat cgacggacag
     9361 taacgaggcc ccactccctt gaccacacct gtaaacatag gcgcacggtg gaggttgttt
     9421 tggataatct catgactggt accattggtg taggtcaacc agcatggcac ttggtctttg
     9481 acataatcct catcacgtga agtgtatgag aagtaattag gtgcttcatc tcctggttgg
     9541 atttctgtca cgtcgtagtt gatagaagag gccttgacac gtggaggggt tcctgtcttg
     9601 aaacgaccga tttcgaggcc cagttccttg agattgtcag ctaggttaat agaagccaag
     9661 ctgtggttag gacctgatga gtacttgagg tctccgatga taatttcccc acggagagca
     9721 gtccctgtcg tcacaataac agccttagca gcatattctt gatgggtggc tgtacgcaca
     9781 ccgacaacct tgccatcttc caccaaaatc tcatcaatca tggtttgacg aagggtcaga
     9841 ttttcttgat tttcaactgt cttgcgcatc tccttagagt aaagttcctt gtcagcctgc
     9901 gcacgaagag cacggacagc tggccccttc cctgtgttga gcatcttcat ctggatgtaa
     9961 gtcttgtcaa tggtcttggc catctcgcca ccgagggcat cgacttcacg cacgacaatc
    10021 cccttggcag aaccaccgat agagggatta caaggcatga aagccagcat ttcaatattg
    10081 atggtcgcaa gcaggacctt acagcccata cggctagcgg ccaaggaagc ctcaacccca
    10141 gcgtgtcccg caccaattac aataatatcg tattcttcag taaaatgata agtcatgttt
    10201 ctctcctatt cctcaagatg aatgtgtctt agttggcctt tccaatctgg tagggccgtt
    10261 tttaaaaaag ctggaactag ctggatatcc tcaagcttat ccaagtcaat ccactcacag
    10321 ggctgccttt tctcatcttc ctgcatggtc aacggggcat cttcaagcaa atccaccaga
    10381 taatgaaact cgatattgtg ataggaaacg ccgtccactt caaaacgatt ttcaaccaca
    10441 aaagctagct gcccagcttg agatttaaca cccagttctt ccttcacttc acggactacc
    10501 gcgtcttccg ttttttcgtt aacttgaatc gcaccgccga taatgtaata cttgcccttg
    10561 tctttggtga ctagaagctt gcgattttga agaatcaaag ctgtcgcccg aacaccaaaa
    10621 accgtattgt ctacttttgt ccgaaagtct tgttgagtca ttcttgtcct ttcccttaaa
    10681 cgacacaaaa acagtcaaaa ctacaaagaa gtgcaggaca aaaaagcctg caacatccat
    10741 gagctttgac catcatttct attgctgtta ttattgtagc aaattgagaa aatttgtcaa
    10801 tctaaatgta ctgacaaact tgtccatcgc ggtaagcatt gtcaatcaaa ccgccaccta
    10861 gacactcttc gccatcgtaa aagacaactg cctgtcctgg tgtaatcgcg cgttgtggtt
    10921 ccgcaaagat aacttctgcc ttgtctcctt tgacatggac ggtcacctta gagtcaggct
    10981 gacggtaacg gaatttagcc gtacattcta gcgtaaactc ttctggcatt tcacgagtaa
    11041 agtggacttg actggcttct aggctagttg acatgagcga atcatggtag aatccttgtc
    11101 ctacatagag aatattcttg cttagatctt ttccgacaac gaaccaaggg gcattgtcac
    11161 cgccgtgttg cccaccgata ccgagtccgc cacgctgacc gattgtatag tacataagac
    11221 ctgcatgctc gcccatatcg cgaccatcca cagtcatcat gcgaccaggc tgagctggca
    11281 ggtagttgct gagaaagttt ttaaagttct tttctccgat aaagcaaatc cctgtcgagt
    11341 ctttcttctt agcagtcgaa aggcctgctt cttctgctag tctgcgtact tcaggctttt
    11401 ccaaatgtcc cagagggaac atggtttttt ggagttgttc ttgcgaaagt tggctgagga
    11461 aataggtctg gtccttgcca ttgtccacgc cacgaagcat gtgaacagtg ccatcctcat
    11521 cacgcgccac tcgagcatag tgcccagtcg ctacatagtc tgcccccaag gtcatggcat
    11581 agtccaaaaa ggccttgaac ttgatttcct tgttgcacat aacgtccgga tttggcgtgc
    11641 gccctgcacg gtattccgct aggaaatact caaaaacgcg gtcccagtac tctttttcaa
    11701 aattgacaga gtagtaggga atgccaatct ggtctgccac cgcaaccaca tccttgtaat
    11761 cttcggtcgc cgtacagacg ccgttttcat ctgtgtcatc ccagttcttc atgaagatac
    11821 cgatcacatc gtagccctgc tccttgagca aaagagccgt caccgacgaa tcaacaccac
    11881 cactcatccc cacaacaaca cgtgttttag agttatcact catggtaagt ctcccatcta
    11941 ttcgtttatt cacgattgaa ggtcgtgtgt gcttcacgat tgaaggtcgc ttgagcagta
    12001 ttcattataa cacgcttggt tagagaagac aaggaagagc ttaaaaattg ttttttaaaa
    12061 cttatagtca atatacagat tatgtgaatg acgtgagtat accttcttct tatcttcatt
    12121 caaataacta taataaataa aacaacccat tttctctaat ctatttttca agttagag
//
LOCUS       DAVKGC010000056        12089 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKGC010000056 DAVKGC010000000
VERSION     DAVKGC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12089
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.056"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(604..1797)
                     /locus_tag="VW049_001292"
     CDS             complement(604..1797)
                     /locus_tag="VW049_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9432410.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(1935..3377)
                     /gene="gtfA"
                     /locus_tag="VW049_001293"
     CDS             complement(1935..3377)
                     /gene="gtfA"
                     /locus_tag="VW049_001293"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9432411.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYALNEFYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            complement(3688..4521)
                     /locus_tag="VW049_001294"
     CDS             complement(3688..4521)
                     /locus_tag="VW049_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9432412.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAIMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(4535..5401)
                     /locus_tag="VW049_001295"
     CDS             complement(4535..5401)
                     /locus_tag="VW049_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9432413.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAAFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(5415..6674)
                     /locus_tag="VW049_001296"
     CDS             complement(5415..6674)
                     /locus_tag="VW049_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9432414.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISVTTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(6766..8928)
                     /locus_tag="VW049_001297"
     CDS             complement(6766..8928)
                     /locus_tag="VW049_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9432415.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            9036..9897
                     /locus_tag="VW049_001298"
                     /pseudo
     CDS             9036..9897
                     /locus_tag="VW049_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            9894..10829
                     /locus_tag="VW049_001299"
     CDS             9894..10829
                     /locus_tag="VW049_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9432416.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRTFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(10955..11042)
                     /locus_tag="VW049_001300"
     tRNA            complement(10955..11042)
                     /locus_tag="VW049_001300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11006..11008),aa:Ser,seq:gct)
     gene            complement(11049..11122)
                     /locus_tag="VW049_001301"
     tRNA            complement(11049..11122)
                     /locus_tag="VW049_001301"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11086..11088),aa:Ile,seq:gat)
     gene            complement(11158..11228)
                     /locus_tag="VW049_001302"
     tRNA            complement(11158..11228)
                     /locus_tag="VW049_001302"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11194..11196),aa:Gly,seq:tcc)
     gene            complement(11248..11320)
                     /locus_tag="VW049_001303"
     tRNA            complement(11248..11320)
                     /locus_tag="VW049_001303"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11285..11287),aa:Phe,seq:gaa)
     gene            complement(11324..11397)
                     /locus_tag="VW049_001304"
     tRNA            complement(11324..11397)
                     /locus_tag="VW049_001304"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11361..11363),aa:Met,seq:cat)
     gene            complement(11408..11497)
                     /locus_tag="VW049_001305"
     tRNA            complement(11408..11497)
                     /locus_tag="VW049_001305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11459..11461),aa:Ser,seq:tga)
     gene            complement(11508..11581)
                     /locus_tag="VW049_001306"
     tRNA            complement(11508..11581)
                     /locus_tag="VW049_001306"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11545..11547),aa:Ile,seq:cat)
     gene            complement(11595..11668)
                     /locus_tag="VW049_001307"
     tRNA            complement(11595..11668)
                     /locus_tag="VW049_001307"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11632..11634),aa:Met,seq:cat)
     gene            complement(11674..11747)
                     /locus_tag="VW049_001308"
     tRNA            complement(11674..11747)
                     /locus_tag="VW049_001308"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11711..11713),aa:Pro,seq:tgg)
     gene            complement(11763..11836)
                     /locus_tag="VW049_001309"
     tRNA            complement(11763..11836)
                     /locus_tag="VW049_001309"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11800..11802),aa:Arg,seq:acg)
     gene            complement(11846..11931)
                     /locus_tag="VW049_001310"
     tRNA            complement(11846..11931)
                     /locus_tag="VW049_001310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11895..11897),aa:Leu,seq:taa)
     gene            complement(11939..12010)
                     /locus_tag="VW049_001311"
     tRNA            complement(11939..12010)
                     /locus_tag="VW049_001311"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11976..11978),aa:Gly,seq:gcc)
BASE COUNT         3858 a         2633 c         2147 g         3451 t
ORIGIN      
        1 aatatttagg cttgttggca caaatttttc attttttttg aatatatgat tcaaattgtc
       61 gttcgaagtg tcaaagacta cagtgaaaat aggaaatttg acacagaaat tttggagctt
      121 aggaagacat acagtaaaat gaaatacgga cggaacaatg tgattttgga attcaaatta
      181 aattataaca atattgtaga agtatcattc tagtattcaa gattcagttt actatgtctt
      241 ttcacaccaa ccttattccg aattcaatta ctttcgtgat ttacacatat agattaagac
      301 tatcttttat actttaaaat ttctcgctac cttgtccact atatgctcct cgctatcacg
      361 tttctattca tagcctacga tttcactatt gctttctctg acaattctta tttcctgcgt
      421 cagacttaaa acgatctatc cccagaccat tttaatccgc tacctcacga tagtcaggct
      481 tggggagcgc tattgtattc accggtagtg gatccctaca gaggacttac acctcagatg
      541 cacgacatgc ccatcgtata aaaaatctcc tacccaaggt agaagatttc aaacttataa
      601 aacttaatcc gtcatgtccg ataccaacat tcgatgctcc aatggaatac tgcacataac
      661 tagcaagaaa ataaagcctg actgaatcca gaagagagcc aagtcaaaaa ttccgtgcac
      721 agcaaccact gtaaggaaag atagataaag gccgataatc ggacgtttcc ccgactcctg
      781 actcatatcc atcatcaaac gaacaggagc aacagaagac agaactaata aaatggtacc
      841 cacaattccg taactcagaa tcgtatcaat ataaagactg tgggcatgtt catgataagg
      901 agcatgtatc cgaggataag agtgcatata ggtcaatggc ccttcacccc aaaaaggatt
      961 ttgcttaaac aaggccatcc cagcatccca gatagaaatg cgttcttcca tagaagagtc
     1021 taaagtaccc attcgaactc ccaaatcact agaaaagagg aaactcaaac caatcgcgaa
     1081 gaccccaata ctaagccaaa aagccttcca gtttttaata gtcgtaaaga gatagataat
     1141 tgctccagcg ataatagcag gaaaggcagt tcgattttga gtaaagttca aaccaaagag
     1201 attaacaaag cctgcaatca cacagaatac tttcaaccaa ttcaacttgg tcgttgtaaa
     1261 cagatagaaa gcaatcataa tacagaaaca acaaataatt ccataataat taggattaaa
     1321 gaaggtcact tctgcccggt tctgatgcca cacctgcata ttgggtgaaa gaaaagcata
     1381 gttaaatttc ttcacaattt ggaaatgttc taaactggca aaagcagctg acaagacact
     1441 accaaacaag acgaactgca aaatcaatcg aaagaattta tgggataaaa tcgactgata
     1501 gtgcaaaaag aaaatagtaa atagaaacat tcctactgaa gccacaagac ccatccaatt
     1561 ttgtgcaaga atggatataa cagtactata gctaagaaaa agaagcagca tcggatgctc
     1621 ccccattttc tgaagaatac ttttcatgtc tcctgtaaaa atcaaactga taatatataa
     1681 acagagtaca actacaaaaa gataaaaggg taaaaagata ctcaggataa ttcccaataa
     1741 aatcagctct ttactagaca accccttcag cttttcaata aagcctattg atttcaaaat
     1801 gaatcctttc tctccaaatc agctgattca gataatagta agctatccta tattgtacca
     1861 cttttttagc aatttgaaaa caaaggaaac gttttccaaa ataaaaaccc tattttatcc
     1921 accatatcaa ggcttcaaaa tgttacttca actccattct caattacccg ataagtctga
     1981 ttttgcaaat caatttctgc tactgctgtt acggacttat ctttattttg acgtttgatt
     2041 acaatgctgt gagctgttgg tgtctctatc tcagtagtcc cttctagatc aaaggcttct
     2101 gaacggttac ggaaagaaaa tagattgaga agggccttca caacaggtcg ttgcacttct
     2161 tttgctattt cctcgttgct atagtaatga cgattaatat ttcgaccttc tttagtttct
     2221 tctaataatt tcaagtcatt cttgcctgct aatagaccca catagtaaac ctgaggaata
     2281 cctggggcaa aagcttgaat tagacgagcg agaaaatact tgacatcatc atctccaagc
     2341 gctgaatagt aggttgaatt gatttggtag atatctaagt tgttatactc ggcactagag
     2401 tacttacgtt tgacattggc tccaacctta tagaattcat ttaaagcata gtcaatctcc
     2461 tcatcggtca ggatatcctt gacatctact actccaatcc catcatgggt atctagcgtc
     2521 gtaaattgct tcatcgggct catctttaac cacttagcca aacgctctgt tctggaactg
     2581 taaagagtat aaagtgtcac cattggaaga gcaaaatcat aaacatagta atcatggtct
     2641 gctattttaa actgaatcga atagtgttca tgaatctcag gtaaaagctc tgtcccatac
     2701 tcagcagcga tatctcgaac tttgtccaat aaatcccaaa tatctggttc cacaaagaaa
     2761 tcattagtat ccaatttctt cactgcataa gcaaaggcat ctagacgaat caaatcacac
     2821 ccattacttg ccaagtgctg aatggtctta cggataaatt ccatagttac ttctttggtc
     2881 acatcaagat caatctgctc ctcaccaaag gtattccaca aatgttccac tgaaccatct
     2941 tcaaacacaa tctcttgctt tggtgcacga tccttacgct tgtaaattaa atctacatca
     3001 gactgtgtcg gacggttttc tggccaaaac ttatcccagt ttaaaaagag atctttaaat
     3061 tcactggctt catgtttttc ttgatagtcc ttataatact tggattgacg agaaatatga
     3121 ttaatcataa aatcaaacat aagataatat ctctcaccta aacgcttaac atcctcccaa
     3181 tcaccaaaag ctgagtccac ttcgtcgtag tcaactggcg caaatccacg atcacctgtt
     3241 gatgggaaaa atggtaaaag gtgaactcct ccaatagcat ctccaaaatg ctcttccaaa
     3301 ttatcatata agtctttaag attatttcca aggctatcag aataggtaat caacatggtt
     3361 ttattttgaa ttggcatcat tactctcctt tttctaattg aagccaagtc tcatatgatc
     3421 tggcttcata aataaaattc attttaaatc tctatttatc atcaaactcg tactaatata
     3481 gactgtgata aacaaagtac tactttcttg ttttctacat agaattatca acaagctgaa
     3541 ctcttcctct gtgtcaaaga ctatagattc catgagctct tcttatactc ttcgaaaatc
     3601 tcttcaaacc acgtcagcgt cgccttaccg tactcaagta cagcctgcgg ctagcttcct
     3661 agtttgctct ttgattttca ttgagtatta cttcactgcc ccgttgctca ttcctgaaat
     3721 gatatggcgt tggaagaaga gatagacaat ggtgatactg ataatgccga ccacgtaaga
     3781 ggcaaagctt ggtccgtagt cgttgaaata ttggcctgcg tagttgtatt ggaacaaagg
     3841 cagagtccac attttggaat cccggttcaa gacaaggagt ggcaacatga agtcattcca
     3901 gaaccaaagg gcattgatga tcatggttgt cgcatgcatc ggtttcatca ttgggaagat
     3961 gatgcggaaa taggttgtaa attgattagc cccatcgatc tctgctgctt catccagact
     4021 ttctggaatc gagattttga tatagccaac atagagaaag agggtctgtg gaatcgcata
     4081 ggtcaagtag agcaagatca aaccaaaggt attagccaaa ccgagtttac tcatcataac
     4141 cgtaatcgga atcatgatga cttggaaagg tacgaagatt ccgaggatta agagggtata
     4201 catgatggta aaggcttttc ttttactcat attgcgagcg atggagtagg ctgccatagg
     4261 gataaagatc attattgcaa gtaaagacaa gacagtgatg acgacagagt tccaataata
     4321 gcctccaatc ccatcagcta agagacggct aaagttgtcc catgtgaagt tggttggaaa
     4381 gccaaagaaa ttatctacaa tatccttagt gggtttgaag gaactaaaga gggtagcaag
     4441 gagcggcact aaaatcagaa ccgatcctag aatcaataga atgtatttgc caatcagggc
     4501 ttttctttca tcttgtttca tcatgcttct cctcttaaat ttcaaatttc ttagatactc
     4561 tcaattggat gatcgaaatc actacaatta agaggaacaa gattacggca atggcattgg
     4621 cataaccgaa ttggttgttt ttaaaggcat agttataaac caagagccca agtgaggttg
     4681 tggcattgtt tggaccacca ccggtcatgg caaagacttg gtcaaaggca gtcagcccac
     4741 cttttagggc taggataaag accatagaga cacttggtag caagtaaggc aattcaatgt
     4801 tccagaaaac ttgcttgcta gtcgcaccat caatccttgc tgcctctgta atctcagttg
     4861 gaatagattg caaaccagct aggaagatga tgatgggcat agccacccct tgccaaagaa
     4921 ggacaaaggc agccgcaaag attgctcccc acttagtccc taaaagactg gtttggagaa
     4981 attcaatatg aagggcattc ccaatcgctg gaagaccgta gttgaagact tgcttgaaga
     5041 tcaaagccac tgtcaaacca gataaaacag ctgggaagaa gaaccaagca cggaagaagg
     5101 tttggccttt gattttagag ttcaagacac gcgcaatgaa gatcccgagt gcaatctcac
     5161 caaccaccat ggcaatcgta atgattgcgg taaagccaat cgcattcatg aattttggat
     5221 ccatgaagag gagcttaaag ttgtttaagc cgacaaattt gtagttataa gtcaatcctg
     5281 tccagttggt aaaactgtaa aaggctcctt gaaacatcgg cacatagaag aaaattgctt
     5341 gtaacaagag ggggatgacc acaaaagccc atgcccaata tttttgtaat acttttttca
     5401 tagtctctct actcctaatc cacatccgct ttcatcgggt taaagaaggc attcaaatca
     5461 ttgaccatgc cttgtttatc accggtcaag acatagttca tggtcaaggt atggaagtct
     5521 gcttcactgg tccagtattg ttgcaaccag accaagtgac gatccgtaaa ggcatattcg
     5581 gtcataccag caagcggtga atcttctcct gcttgtttga ccccttcgat cgctgttgga
     5641 gatccgtcca catcgtagta tttttgcatg acttctggac gggtcatata ttccacaaag
     5701 gcattggctt cttttggatg tttggtggtg actgagatag accatgccaa gtctcccgca
     5761 ccaacggtta agctttgtcc tttttctttt cctggaatca tgaaggtccc aatcttaaag
     5821 ttcggttttt gttcattaat cgctgtgatc gcccaagacc catttggtgt catgaggaca
     5881 tccccacgtg cgaaggctcc gataacatcg gtatagccag caccttccca gttcttttgc
     5941 ttagagccat tgatgcgaag gatgtccatg accttgatat catctttcat aatcggatcc
     6001 gacaatttaa tggcatttgg ttgagaataa cgaaggtatt gatttgcttc ttttcctcca
     6061 cctgttgctg tcgcaaaggc taattgattg taaccattga gtgtccaagc atctgcacct
     6121 gcaattccaa atggtgtttg tcctttagca acgatatctt tgactaactg ttcaaattca
     6181 tcccaggttt caggaacctt caagcccagt tcttcgaatt tatctttgtt gtagtaaatt
     6241 ccataagcat tagctgtaaa aggaacgttg taaacttttt cgtttacagc atatttttca
     6301 gcgtagccat ttttcacgcg tttcaggtag tctttgttgc tcaaatcttc aaaaacacct
     6361 gcttttgccc attcttgcag ttcgatggac tgtgggtaaa tattgaccac atcaggcaca
     6421 tctcctgcga gaacgcgtgt cttcaatact tcaccagcat ttggtacatt gacgactttg
     6481 accttgatct tagggttttc cttctcaaaa tcacgagtga tttcttccaa ggttttggtc
     6541 atttcttttt tctggttgaa atactcgatg gtcactgtgc catccgcaga tttaccatag
     6601 ttggagcaag cgccgagccc aaacaaagct aaacctgtag ttgcaagaag tccgattttt
     6661 ttataccatt ccattagaaa gcctccttta taaatttata cacccttatt gaactgcacc
     6721 ccaaaagtta gacagaataa atctaacttt tggggtcagt acatatcata gttttctaaa
     6781 aatatactgt ctactcaaaa aatctccttg ggataagata acagttaagc ccgcatacat
     6841 tagttctgca cctgagtaaa cttcgccatt ttcctgtaat ttatatagtc cctcttcatc
     6901 caaatctttt aattttaaag ttgtttccat ggtctctaca acagataaaa cgcgaacgta
     6961 ggttacaatc gtttgatttc cgtaattaaa ttgtacagct gcttcattgg atgcagtatc
     7021 aggattaatt agtctatact gctgtcctaa ctgaactact ggtcgtaatt ctttatacaa
     7081 gttcacctga ttagcaatcg tagctttctc ttcatctgat aaatttgtca aatcaagctc
     7141 atagcccaaa tttcccatca ttgctacatg gccacgtgtt tctaatggtg tcattcgtcc
     7201 catctgatga ttcggtactg ctgacacatg agcccccata gaaatggttg gatagagata
     7261 ggatgaaccg tattgaattg gtaaacgtgc aatggcatca gtattatcac tagcccagac
     7321 ttgtgggaaa tagcgtatca taccaagatc atttcgtcca ccaccaccag agcaggactc
     7381 aaagagaata tggctgtgct tctctgtcag ataagaaacg agttcgtaaa gtcccagcat
     7441 gtactgatga gattgcatct gtgtctctag ataagttaat ccattcccta gcttagtgat
     7501 attgcggttc atatcccatt taatgtaatc aatctcatga taagatagga gttgatctaa
     7561 gacacttttc aagtattcta ctacctgagg attggcaaga ttaagtacta attgattccg
     7621 agaataagta tgctcataat caggaacctg aatagcccag tcaggatgtt gacgatacaa
     7681 atcactatct acagaaatca tttcgggttc taaccaaagt ccaaactgca aacctctttc
     7741 atggatagct gaaatcagac tttctagact tccacccagt ttttcctcat taacaaccca
     7801 atcacctaaa gcacgattat catcaaaacg attgccaaac caaccatcat ctaatacaaa
     7861 aagttcaatg ccaactttct tagcttcatc tgctaactct aacagttttt ctctctgaaa
     7921 gtcaaagtaa gtagcttccc agttattgat tagaattgga cgttcttttt tagaaaattc
     7981 acttagcata atgtgcttca gtacaaaatt ctgactttca tgactaatac cagttaatcc
     8041 ctgatctgaa tgagtcacta aagctaccgg tgtttcaaag tattcctcag gggctaactt
     8101 ccaagaaaag ttttctggat taatgccaat agccacccga acttcattca attgattttt
     8161 ttgaacaaaa gcttcaaagt tgccactata cattagttga atagcaaaca cattcccagc
     8221 atcctctgtg actccttgtt cgcatagtag aagagctggt gtttgagcat gaccagaagc
     8281 acctcggttt gagctaatcg aaaagattcc ttgttctacc tgttgacgtc taacagtctt
     8341 ttcacgagca taagcaccct gcagagttac tatttcgtaa tctgcagctg gaaaatcagc
     8401 cataaaagaa aaatctttat ggatgacaac ttcctgatta ctattattat ctaatttact
     8461 gtagctagca atagtcgcat cattattaaa agtagtataa tacaaagtca gactaagttg
     8521 agccttagaa tcttctaaca ttaagacaag agtctctgta tcgtccatgc tatgtggaga
     8581 aggtaagccc tgtggaccat tctgaccttt taaaatcttt gcttctacaa atcgaaagtc
     8641 tgttacttca gttacactat gctgaacctg tatggttggt ttcctaaaat ctcctaagcc
     8701 atgttgtcca aaaatctgtc gctgagtatc taaactaaag gttcgattag tagccgttgg
     8761 atttcctgaa aaggcatggt ctcgttcata aacactattg gaacctttat agttcttaat
     8821 agtctttcct aaatgtttca aaagtaagta gccatttcga ttttcaataa tcaaacttag
     8881 atttttactc tcaacataaa atagattatt ctctatccta actcccattt acttcacctc
     8941 atcactttat tgattatatt ttatcacctg aaatcgcttt ccaaaataga aaaatgtctc
     9001 aagaatatgg taaaatgtta ggtaggaggt agcacatgtt agttttttca gaataccaga
     9061 ctggaacaat cgaccttgcc ctaagctttt atggatatga ggaatgcaca cctaattact
     9121 cttttggtcc agccattcgt gatacatacg ttctacatta cattactaaa ggacaaggaa
     9181 aatttcatta caagggtaaa attgttgatt taaaagaagg agatttcttt ctattaaaac
     9241 cagaggaact aaccttttat caagcagata gtaaagaacc ttgggcctac tactggttag
     9301 gaatcactgg agggaaagcc cctgattatt ttgctctttc ccaaatttct gatcaatcct
     9361 atctcatcca atctgaaact tgtcataccc agactactgc aaaactcatc tcagacattg
     9421 tccgcttcgc tcagattaca aaatcaagtg aattagctca actccatatc atgggacaac
     9481 ttcatgaact gatgtttcat ctgggaacta ttgctcccaa tcagaaaaaa aaagaatatt
     9541 tcatcaaccc accaactcta tcttgaatgc aaacgattaa ttgatagcca ctatcctcaa
     9601 tcacttacaa ttcaagattt agcaaaagaa ctatccgttc acagaagcta cttatcaagc
     9661 gtattcaaag aatttaatac cttatcaccc aaagaatacc tactctacgt tcgaatgcac
     9721 cgagctagac aacttctcga aaatacccaa gagtccatca aggtaattgc atactcggta
     9781 ggtttttcag atccactcca tttttcgaaa gcttataaac aatactttaa tcagactcca
     9841 agtcatacaa gaaaagaata ctctcaatac caactagtaa gaaaggcaac attatgaaat
     9901 cctaccaagc tgtctaccaa atcctatcta aagaaaccga ctatatcagc ggagaaaaaa
     9961 tcgcagaaaa actatcccta agccgaacag caatttggaa agctatcaag cgactagaac
    10021 aagaaggcat tgaaattgat agtatcaaaa atagaggata taaactgatg aatggtgacc
    10081 ttattcttcc agagattcta gaagaaaatc ttccaattaa agtcagcttt aaacccgaaa
    10141 caaaatcaac acaactagat gcaaaagaag caattgattt aggccatgaa gcaaataccc
    10201 tctatctagc ttcctatcaa acagcaggcc gaggccgttt tcaacgttcc ttctactcac
    10261 cacaaggtgg tatttatatg acactccatc ttaaaccaaa tctcccctat gacaaattac
    10321 catcctacac actacttgta gctggagctg tctacaaagc cattaagaac ctaactttaa
    10381 tagatgtcga cataaaatgg gtcaatgata tctatctaaa caatcataaa attggaggaa
    10441 tccttactga agcaatgacc tctgtagaaa ctggcttagt cacagatatc attattggag
    10501 taggtatcaa tttcactatt aaagacttcc ctcaggaatt aaaagaaaaa gctgccagct
    10561 tatttaaagc tacagctcct ataacaagga atgaattgat catagaaatc tggcgtactt
    10621 tcttcgaaac accagcagaa gagctattat acctatacaa aaaacagtca ttcattctag
    10681 gaaaagaagt cactttcaca ctagagcaaa aagactacaa gggacttgct aaagacatct
    10741 cagaaaatgg aaaactttta gttcaatgtg ataacggaaa agaaatctgg ctaaatagtg
    10801 gcgaaatttc tctcaatagt tggaagtaaa ataacacaat tataatataa acgatataaa
    10861 aataacttca gattagtaat tcaattaagt tttacggatc tgaagtttta ttgtctctaa
    10921 aaataaaaaa gagagttaca aactctcatt aaaacggaga ataagggatt cgaacccttg
    10981 cgccagttac ccgacctaac gatttagcaa accgtcctct tcagcctctt gagtaattct
    11041 ccaattaatg ggcacgagtg gactcgaacc accgacctca cgcttatcag gcgtgcgctc
    11101 taaccacctg agctacgcgc ccaagttaaa aaacttggta atttgaacaa agttcaaagc
    11161 gggtgacgag aatcgaactc gcgacaacag cttggaaggc tgtagtttta ccactaaact
    11221 acacccgcat aaatactatc aataaaatgg cgcgagacgg aatcgaaccg ccgacacatg
    11281 gagcttcaat ccattgctct accaactgag ctaccgagcc ttattgcggg agcaggattt
    11341 gaacctacga ccttcgggtt atgagcccga cgagctaccg agctgctcca tcccgcgtta
    11401 atataaaagg aggatgtggg attcgaaccc acgcacgctt ttacacgcct gacggttttc
    11461 aagaccgttc ccttcagccg gacttgggta atcctccaat attcaaatgg accttgtagg
    11521 acttgaacct acgaccactc ggttatgagc cgagagctct aaccagctga gctaaaggtc
    11581 cgacaagatc attatagcgg cgaaggggat cgaacccccg acctcccggg tatgaaccgg
    11641 acgctctagc cagctgagct acaccgccat gaatcgggaa gacaggattc gaacctgcga
    11701 caccttggtc ccaaaccaag tactctacca agctgagcta cttcccgagt taaatagaaa
    11761 aatgcaccct agaggagtcg aacctctaac cgcctgattc gtagtcaggt actctatcca
    11821 gttgagctaa gggtgctcca tattatgccg aggaccggaa tcgaaccggt acgatcgtta
    11881 ccaatcgcag gattttaagt cctgtgcgtc tgccagttcc gccaccccgg cctctctaag
    11941 cgaacgacgg gattcgaacc cgcgaccccc accttggcaa ggtggtgttc taccactgaa
    12001 ctacgttcgc actgttttct tctatctaaa aatgccggct acatgacttg aacacgcgac
    12061 cctctgatta caaatcagat gctctacca
//
LOCUS       DAVKGC010000057        11908 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKGC010000057 DAVKGC010000000
VERSION     DAVKGC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.057"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            62..283
                     /locus_tag="VW049_001312"
     CDS             62..283
                     /locus_tag="VW049_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU9432417.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            337..1008
                     /locus_tag="VW049_001313"
     CDS             337..1008
                     /locus_tag="VW049_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9432418.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVIMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(1060..1257)
                     /locus_tag="VW049_001314"
                     /pseudo
     CDS             complement(1060..1257)
                     /locus_tag="VW049_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1250..2158
                     /locus_tag="VW049_001315"
     CDS             1250..2158
                     /locus_tag="VW049_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9432419.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2155..2616
                     /locus_tag="VW049_001316"
     CDS             2155..2616
                     /locus_tag="VW049_001316"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9432420.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2606..3493
                     /locus_tag="VW049_001317"
     CDS             2606..3493
                     /locus_tag="VW049_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9432421.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3496..5379
                     /gene="cbpE"
                     /locus_tag="VW049_001318"
                     /gene_synonym="pce"
     CDS             3496..5379
                     /gene="cbpE"
                     /locus_tag="VW049_001318"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9432422.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPILS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIAGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5459..6589
                     /locus_tag="VW049_001319"
     CDS             5459..6589
                     /locus_tag="VW049_001319"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9432423.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRKI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6599..7861
                     /locus_tag="VW049_001320"
     CDS             6599..7861
                     /locus_tag="VW049_001320"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9432424.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7865..8662
                     /gene="proC"
                     /locus_tag="VW049_001321"
     CDS             7865..8662
                     /gene="proC"
                     /locus_tag="VW049_001321"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9432425.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFYQLLAKAGLLVELGES
                     LIDAATGLAGCGPAFVYLFIEALADAGVQTGLPREIALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            8882..9520
                     /locus_tag="VW049_001322"
     CDS             8882..9520
                     /locus_tag="VW049_001322"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9432426.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            9517..10407
                     /locus_tag="VW049_001323"
     CDS             9517..10407
                     /locus_tag="VW049_001323"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9432427.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10447..10764
                     /gene="yabA"
                     /locus_tag="VW049_001324"
     CDS             10447..10764
                     /gene="yabA"
                     /locus_tag="VW049_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9432428.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10767..11636
                     /gene="rsmI"
                     /locus_tag="VW049_001325"
     CDS             10767..11636
                     /gene="rsmI"
                     /locus_tag="VW049_001325"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9432429.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
BASE COUNT         3538 a         2053 c         2849 g         3468 t
ORIGIN      
        1 gtaagtaggt atgagtatca gattactcaa ctaatctgaa gaataatgga ggaaatatat
       61 catgatttta atgacaaaaa atataaatct aacaaatgaa gaattagagc tgatacaagg
      121 tggagcagat tcatatggta aaaatcctaa tggtaggtac gattgggaaa tagaaccagt
      181 attaactctg ccggttcatg gattttgtcc cagaggcacc tatgatttag gatatattgg
      241 aggaggcaat catctttgca aaggaagtgc tgcgagattt taagtaaaat ttattagtaa
      301 tatgaagaaa caagtggaga aagcagagaa tttaatatga aaaaacgggc tattcaaatt
      361 ttactagcat tgtccttaat tttttacaaa tcaacttggt tttggagagt tttcaatcac
      421 ctcgcaaagc cctatctacc agcaagtcgt gaattttttc agattctgct tttgatggag
      481 agcggagttc ttttcttagc ggtcatctat ctactggttt ttgcaggaaa gaaaattttt
      541 catttcaagt ggcagctgag gtacttcatc taccttttac tgggctacat catttcatat
      601 atgtctgact tcctcttttc gtatttcata tccctgtctt caaatcagat ttctttgaat
      661 gaaacggtag aaatgatggg gagacaggag ttcccttatt tcttgctcat cgtttgcttc
      721 atcgccccta ttgctgagga attgatttat cgcggtgtga ttatgacaac ctttttcaaa
      781 aactcgcctt ggtacggaga tgttttgctt tctgctatta ttttcggtta tattcatatc
      841 aattttgctt taactcctct tgcttttttc atttatgcta gtggaggtct tattttagct
      901 ctattgtatc gcatgactaa aaatctctac tatccaatac tagttcatat tctcattaat
      961 atcactgcct tctgggatgt gtggttgctc ctattttcag gaagttagct tactaaaata
     1021 atgtcggaac tttccggcat tttctttttt cacaaatagt caacgttttt cttttcgata
     1081 ttgtagtggt gtgtagccag ttattttttt gaattgattt tgaaaataag gttggcttga
     1141 gaaaggcaga tagtgaagat agttaagaag aataggatgt tcttttttcc tttttggaaa
     1201 acttctaaaa tatggtataa tgaaaagata aagaagttag gggtagaaga tgaacattca
     1261 acaattacgc tatgttgtgg ctattgccaa tagtggtact tttcgtgaag ctgctgaaaa
     1321 gatgtatgtt agtcagccga gtctgtctat ttctgttcgg gatttggaaa aagagttggg
     1381 ttttaagatt ttccgtcgga ccagctcagg gactttcttg acccgtcgtg ggatggaatt
     1441 ttatgaaaaa gcgcaagaat tggttaaagg atttgatatt tttcaaaatc agtatgccaa
     1501 tcctgaagaa gaaaaagatg aattttccgt tgctagccag cactatgact tcttaccacc
     1561 aactattacg gccttttcag agcgctatcc tgactataag aacttccgta tttttgaatc
     1621 aactactgtt caaatattag atgaagtggc gcaagggcat agtgagattg ggattatcta
     1681 cctcaacaat caaaataaaa aggggattat gcaacgggtt gaaaagttag gtctggaggt
     1741 catcgaattg attcctttcc atacccatat ttatctctgt gagggtcatc ctttagccca
     1801 gaaagaggaa ttagtcatgg aggatttagc ggatttacca acggttcgtt tcactcaaga
     1861 gaaagacgag tacctttatt attcagagaa ctttgttgat accagcgcta gctcacagat
     1921 gtttaatgtg acagaccgtg ccaccttgaa tggtattttg gagcggacgg acgcctatgc
     1981 gacaggttct ggatttttag atagtgacag tgttaatggc attacagtta ttcgtctcaa
     2041 ggataaccta gataaccgca tggtctatgt taaacgtgaa gaagtggagc ttagtcaagc
     2101 tgggactctc ttcgtagaag tcatgcaaga atattttgat caaaagagga aatcatgaaa
     2161 aaaagagcaa tagtggcagt cattgtactg cttttaattg ggctggatca gttggtcaaa
     2221 tcctatatcg tccagcagat tccactgggt gaagtgcgct cctggattcc caatttcgtt
     2281 agcttgacct acctgcaaaa tcgaggtgca gccttttcta tcttacaaga tcagcagctg
     2341 ttattcgctg tcattactct ggttgtcgtg ataggtgcca tttggtattt acataaacac
     2401 atggaggact cattctggat ggtcttgggt ttgactctaa taatcgcggg tggtcttgga
     2461 aactttattg acagggtcag tcagggcttt gttgtggata tgttccacct tgactttatc
     2521 aactttgcaa ttttcaatgt ggcagatagc tatctgacgg ttggagtgat tattttattg
     2581 attgcaatgc taaaagagga aataaatgga aattaaaatt gaaactggtg gtctgcgttt
     2641 ggataaggct ttgtcagatt tgtcagaatt atcacgtagt ctcgcgaatg aacaaattaa
     2701 atcaggccag gtcttggtca atggtcaagt caagaaagct aaatacacag tccaagaggg
     2761 tgatgtcgtc acttaccatg tgccagaacc agaggtatta gagtatgtgg ctgaggatct
     2821 tccgctagaa atagtctacc aagatgagga tgtggctgtc gttaacaaac ctcagggaat
     2881 ggttgtgcac ccgagtgctg gtcataccag tggaacccta gtaaatgccc tcatgtatca
     2941 tattaaggac ttgtcgggta tcaatggggt tctgcgtcca gggattgttc accgtattga
     3001 taaggatacg tcaggtcttc tcatgattgc taaaaacgat gatgcgcatc tagtacttgc
     3061 ccaagaactc aaagataaaa agtctctccg caaatattgg gcgattgttc atggaaatct
     3121 gcctaatgat cgtggtgtaa ttgaagcgcc gattggccgg agtgaaaaag accgtaagaa
     3181 acaggctgta actgctaaag ggaagcctgc agtgacgcgt tttcacgtct tggaacgctt
     3241 tggcgattat agcttagtag agttgcaact ggagacaggg cgcactcatc aaatccgtgt
     3301 ccacatggct tatatcggcc atccagtcgc tggtgatgag gtctatggtc ctcgcaagac
     3361 tttgaaagga catggacaat ttcttcatgc caagacttta ggttttactc atccgagaac
     3421 aggtaagacc ttggaattta aagcagatat cccagagatt tttaaggaaa ccttggagag
     3481 attgagaaag taagaatgaa aaagaaatta actagtttag cacttgtagg cgctttttta
     3541 ggtttgtcat ggtatgggaa tgttcaggct caagaaagtt caggaaataa aatccacttt
     3601 atcaatgttc aagaaggtgg cagtgatgcg attattcttg aaagcaatgg acattttgcc
     3661 atggtggata caggagaaga ttatgatttc ccagatggaa gtgattctcg ttatccatgg
     3721 agagaaggaa ttgaaacgtc ttataagcat gttctaacag accgtgtctt tcgtcgtttg
     3781 aaggaattgg gtgtccaaaa acttgatttt attttggtga cccataccca cagtgatcat
     3841 attggaaatg ttgatgaatt actgtctacc tatccagttg accgagtcta tcttaagaaa
     3901 tatagtgata gtcgtattac taattctgaa cgtctatggg ataatctgta tggctatgat
     3961 aaggttttac agactgctgc agaaaaaggt gtttcagtta ttcaaaatat cacacaaggg
     4021 gatgctcatt ttcagtttgg ggacatggat attcagctct ataattatga aaatgaaact
     4081 gattcatcgg gtgaattaaa gaaaatttgg gatgacaatt ccaattcctt gattagcgtg
     4141 gtgaaagtca atggcaagaa aatttacctt gggggcgatt tagataatgt tcatggagca
     4201 gaagacaagt atggtcctct cattggaaaa gttgatttga tgaagtttaa tcatcaccat
     4261 gataccaaca aatcaaatac caaggatttc attaaaaatt tgagtccgag tttgattgtt
     4321 caaacttcgg atagtctacc ttggaaaaat ggtgttgata gtgagtatgt taattggctc
     4381 aaagaacgag gaattgagag aatcaacgca gccagcaaag actatgatgc aacagttttt
     4441 gatattcgaa aagacggttt tgtcaatatt tcaacatcct acaagccgat tctaagtttt
     4501 caagctggtt ggcataagag tgcatatggg aactggtggt atcaagcgcc tgattctaca
     4561 ggagagtatg ctgtcggttg gaatgaaatc gaaggtgaat ggtattactt taaccaaacg
     4621 ggtatcttgt tacagaatca atggaaaaaa tggaacaatc attggttcta tttgacagac
     4681 tctggtgctt ctgctaaaaa ttggaagaaa atcgctggaa tctggtatta ttttaacaaa
     4741 gaaaatcaga tggaaattgg ttgggttcaa gataaagagc agtggtatta tttggatgtt
     4801 gatggttcta tgaagacagg atggcttcaa tatatggggc aatggtatta ctttgctcca
     4861 tcaggggaaa tgaaaatggg ctgggtaaaa gataaagaaa cctggtacta tatggattct
     4921 actggtgtca tgaagacagg tgagatagaa gttgctggtc aacattatta tctggaagat
     4981 tcaggagcta tgaagcaagg ctggcataaa aaggcaaatg attggtattt ctacaagaca
     5041 gacggttcac gagctgtggg ttggatcaag gacaaggata aatggtactt cttgaaagaa
     5101 aatggtcaat tacttgtgaa cggtaagaca ccagaaggtt atactgtgga ttcaagtggt
     5161 gcctggttag tggatgtttc gatcgagaaa tctgctacaa ttaaaactac aagtcattca
     5221 gaaataaaag aatccaaaga agtagtgaaa aaggatcttg aaaataaaga aacgagtcaa
     5281 catgaaagtg ttacaaattt ttcaactagt caagatttga catcctcaac ttcacaaagc
     5341 tctgaaacga gtgtaaacaa atcggaatca gaacagtagt agaaaagaag gttttagggc
     5401 cttctttttc ctatcaactc ttttctattt cctgttattc atgttataat ggataaatat
     5461 gaataatcgg agtgagacta tgaaatacaa acggattgtc tttaaggtgg gtacttcttc
     5521 tctgacaaat gaggatggaa gtttatcacg tagtaaggta aaggatatta cccagcagtt
     5581 ggctatgctg cacgaggctg gtcatgagtt gattttggtg tcttcaggtg ccattgcggc
     5641 tggttttgga gccttaggat ttaaaaagcg tccgactaaa attgctgata aacaggcttc
     5701 agcagcggta gggcagggac ttttgttgga agaatatacg accaatcttc tcttgcgtaa
     5761 aatcgtttct gcacaaatct tgctgaccca agatgacttt gtggataagc gtcgttataa
     5821 aaatgcccat caggctttgt cggttttgct caaccgtggg gcaattccta tcatcaatga
     5881 gaatgatagt gtcgttattg atgagctcaa ggttggggac aatgacactc taagtgctca
     5941 agtagcggcg atggtccaag cagacctttt agttttcttg acagatgtgg acggtctcta
     6001 tactggaaat cctaattcag atccaagagc caaacgcttg gagagaatcg agaccatcaa
     6061 tcgtgagatt attgatatgg ctggtggagc tggttcgtca aacggaactg ggggtatgtt
     6121 aaccaaaatc aaggctgcaa ctatcgcgac ggaatcagga gttcctgttt atatctgctc
     6181 atccttgaaa tcagattcca tgattgaggc ggcagaggag accgaggatg gttcttactt
     6241 tgttgctcaa gagaaggggc ttcgtaccca gaaacaatgg cttgccttct atgctcagag
     6301 tcaaggttct atttgggttg ataaaggggc tgcggaagct ctctctcaac atggaaagag
     6361 tcttctctta tctggtatcg ttgaagcaga aggagccttt tcttacggtg atatcgtgac
     6421 agtatttgac aaggaaagtg gaaaatcact tggaaaagga cgcgtgcaat ttggagcatc
     6481 tgctttggag gatatgttgc gttctcaaaa agccaagggt gtcttgattt accgtgacga
     6541 ctggatttcc attactcctg aaatccaact actttttaca gaattttaga ggtaaactat
     6601 ggtgagtaga caagaacaat ttgaacaggt acaggctgtt aaaaaatcga ttaacacagc
     6661 tagtgaagaa gtgaaaaacc aagccttgct agccatggct gatcacttag tggctgctac
     6721 tgaggaaatt ttagcggcta atgccctcga tatggcagcg gctaagggga aaatctcaga
     6781 tgtgatgttg gatcgtcttt atttggatgc agatcgtata gaagcgatgg caagaggaat
     6841 tcgtgaagtg gttgccttac cagatccaat cggtgaagtt ttagaaacaa gtcagcttga
     6901 aaatggtttg gttatcacaa aaaaacgtgt agctatgggt gtcatcggta ttatctatga
     6961 aagccgtcca aatgtgacgt ctgatgcggc tgctttgact cttaagagtg gaaatgcggt
     7021 tgttcttcgt agtggtaagg atgcctatca aacaacccat gccattgtca cagccttgaa
     7081 gaagggcttg gagacgacta ctattcatcc aaatgtgatt caactggtgg aggatactag
     7141 ccgtgaaagt agttatgcta tgatgaaggc caagggctat ctagaccttc tcattcctcg
     7201 tggaggagct ggcttgatca atgcagtggt tgagaatgcg attgtacctg ttatcgagac
     7261 agggactggg attgtccatg tctatgtgga taaggatgca gacgaagaca aggcgctgtc
     7321 tatcatcaac aatgctaaaa ccagtcgtcc ttctgtttgt aatgccatgg aggttctgct
     7381 ggttcatgaa aacaaggcag caagcatcct tcctcgcttg gatcaaatgc tggttgcaga
     7441 gcgtaaggaa gccggactgg aaccaattca attccgcttg gatagcaaag caagccagtt
     7501 tgtttcaggt caagcagctg agacccaaga ctttgacacc gagtttttag actatgtcct
     7561 tgctgttaag gttgtgagca gtttagaaga agcggttgcg cacattgaat cccacagcac
     7621 ccatcattcg gatgctattg tgacggaaaa tgctgaagct gcagcatact ttacagatca
     7681 agtggactct gcagcggtgt atgttaatgc ctcaactcgt ttcacagatg gaggacaatt
     7741 tggtcttggt tgtgaaatgg ggatttctac tcagaaattg cacgcgcgtg gtcccatggg
     7801 cttgaaagag ttgaccagct acaagtatgt ggttgccggt gatgggcaga taagggagta
     7861 agagatgaag attggattta tcggtttggg gaatatgggt gctagcttgg caaaatctgt
     7921 cttgcagact aggacgtcag atgagattct ccttgccaat cgtagtcaag ctaaggtaga
     7981 tgctttcatt gcagactttg gtggtcaggc ttccagcaat gaagaaatgt ttgcagaagc
     8041 agatgtgatt tttctaggag ttaagcctgc tcagttttct gaactgcttt ctcaatacca
     8101 gaccatcctt gaaaaaagag aaagtcttct tttgatttcg atggcagctg gattgacctt
     8161 agaaaaacta gcaagtctta tcccaagtca acaccgaatt attcgtatga tgcctaatac
     8221 ccctgcttct atcgggcaag gagtgattag ttatgccttg tctcctaatt gcagggctga
     8281 ggacagtgag ctcttttatc agcttttagc caaggctggt ctcttggttg aactaggaga
     8341 aagtttaatc gatgcagcga caggtcttgc aggttgtgga ccagcctttg tctatctttt
     8401 tatcgaggcc ttggcagatg caggtgttca gacaggatta ccacgagaaa tagcattgaa
     8461 aatggcagca caaactgtgg taggagctgg gcaattggtc cttgaaagtc agcaacatcc
     8521 tggagttttg aaagatcaag tttgtagtcc aggcggttcg actattgctg gcgtagcaag
     8581 cctagaagcg catgcttttc gaggcacagt catggaggca gttcatcaag cctataaacg
     8641 aacacaagaa ctaggtaaat aagaggtagc tttgactgcc tcttttatgg tggctgatac
     8701 tcttcgaaaa tcaaattcaa actgcgtcag cttcaccttg ccgtactcaa gtacagcctg
     8761 cggctagctt cctagtttgc tctttgattt tcattgagta tgaaatgaga agacaaaaat
     8821 attgtcacaa acccctattt ttttgataga atagaagtag taaaaaagaa atgagttaga
     8881 catgtcaaaa ggatttttag tctctcttga gggaccagag ggagcaggca agaccagtgt
     8941 tttagaggct ctgctaccaa ttttagagga aaaaggagta gaggtgttga cgacccgtga
     9001 acctggcgga gtcttgattg gggagaagat tcgggaagtg attttggatc caagtcatac
     9061 tcagatggat gctaaaacag agctacttct ctatattgcc agtcgcagac agcatttggt
     9121 ggaaaaagtt cttccagcac ttgaagctgg caagttggtc atcatggatc gttttatcga
     9181 tagttctgtt gcctatcagg gatttggtcg tggcttagat attgaagcca ttgactggct
     9241 caatcagttt gcgacagatg gcctcaaacc cgatttgaca ctctattttg acatcgaggt
     9301 ggaagaaggg ctggctcgta ttgctgctaa tagtgaccgc gaggttaatc gtttggattt
     9361 ggaagggttg gacttgcata aaaaagttcg tcaaggctac ctttctcttc tggataaaga
     9421 gggaaatcgc attgtcaaga ttgatgctag tctccctttg gagcaagttg tggaaactac
     9481 caaggctgtc ttgtttgacg gaatgggctt ggccaaatga aacaagatca actaaaggct
     9541 tggcaaccag ctcagtttga ccgttttgtc cgtatcttag aacaagacca gctcaatcac
     9601 gcctatctct tttcaggttt ctttggaagc ttggaaatag cgcaattttt agctaagagc
     9661 ctcttttgta cggataaagt tggcgtctta ccatgtgaga aatgccgaag ttgcaagctg
     9721 attgaacagg aagagtttcc agatgtcacc ttgattaagc cagtcaatca ggtcatcaag
     9781 acagaacgca ttcgggaatt ggtgggacag ttttctcaag cagggattga aagccagcaa
     9841 caggtcttta tcatcgagca agcggataaa atgcatccca acgcagccaa ttctctgctc
     9901 aaggtcatcg aagaacccca gagtgaagtt tatattttct tcttgactag cgatgaggaa
     9961 aagatgttac cgacaatccg aagtcggact cagatcttcc actttaaaaa gcaagaagaa
    10021 aaacttatct tactcttaga acaaatggga cttgttaaga aaaaagcgac tcttttagct
    10081 aagtttagtc aatcgcgagc tgaagcagaa aagttggcta atcaggcaag tttttggacc
    10141 ttggtcgatg aaagtgaacg cctgctgact tggttagtag ctaagaaaaa agaaagttat
    10201 ctacaggttg ccaaattagc caacttggca gatgataagg aaaaacagga tcaggtttta
    10261 cggattcttg aagttctctg tgggcaggac ctcttgcagg taagagtaag agtgattcta
    10321 caagatttac tagaagctag aaaaatgtgg caagctaatg tcagctttca aaatgccatg
    10381 gaatatctgg tcttgaaaga aatataaact caaaaatgaa tgataaagaa aggaaagggc
    10441 tgttttatgg acaaaaaaga attatttgac gcgctggatg atttttccca acaattattg
    10501 gtaaccttag ccgatgtgga agccatcaag aaaaatctca agagcctggt agaggaaaat
    10561 acagctcttc gcttggaaaa tagtaagttg cgagaacgct tgggtgaggt ggaagcagat
    10621 gctcctgtca aggccaagca tgttcgtgaa agtgtccgtc gcatttaccg tgatggattt
    10681 cacgtatgta atgattttta tggacaacgt cgagagcagg acgaggaatg tatgttttgt
    10741 gacgagttgc tatacaggga gtaggcatgc agattcaaaa aagttttaag gggcagtctc
    10801 cctatggcaa gctgtatcta gtggcaacgc cgattggcaa tctagatgat atgacctttc
    10861 gagctatcca gaccttgaaa gaagtagatt ggattgctgc tgaggatacg cgcaatacag
    10921 gtcttttgct caagcatttt gacatttcca ctaagcagat cagttttcat gagcacaatg
    10981 ccaaggaaaa aattcctgat ttgattggtt tcttgaaagc agggcaaagt attgctcagg
    11041 tctctgatgc cggtttgcct agcatttcag accctggtca tgatttagtt aaggcagcta
    11101 ttgaggaaga aattgcagtt gtgacagttc caggtgcctc tgcaggaatt tctgccttga
    11161 ttgccagtgg tttagcgcca cagccacata tcttttacgg ttttttaccg agaaaatcag
    11221 gtcagcagaa gcaatttttt ggcttgaaaa aagattatcc tgaaacacag attttttatg
    11281 aatcacctca tcgtgtagca gacacgttgg aaaatatgtt agaagtctac ggtgaccgct
    11341 cagttgtctt ggtcagggaa ttgaccaaaa tctatgaaga ataccaacga ggtactatct
    11401 ctgagttatt agaaagcatt gctgaaacgc cactcaaggg cgaatgtctt ctcattgttg
    11461 agggtgccag tcagggtgtg gaggaaaagg acgaggaaga cttgttcgta gaaattcaaa
    11521 cccgcatcca gcaaggtgtg aagaaaaacc aagctatcaa ggaagtcgct aagatttacc
    11581 agtggaataa aagtcagctc tacgctgcct accacgactg ggaagaaaaa caataaaggg
    11641 agacaggatg taataattct gtctgtttct gtttaactta attagtgatg ataatataaa
    11701 gatgtatcac ttggtataga agctttggta ttaagttttt tattaagccc atacggaata
    11761 ccgatggttg gagcagcagt tatagtgttc ctagaaggta taaatagaaa aataaggtca
    11821 ttttaaatca aaggattgat aaatcagaaa gaaggtgatt ttttgcgaac atacgaaaat
    11881 aaagaagaac taaaagctga gatagaga
//
LOCUS       DAVKGC010000058        11514 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKGC010000058 DAVKGC010000000
VERSION     DAVKGC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.058"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            101..754
                     /locus_tag="VW049_001326"
     CDS             101..754
                     /locus_tag="VW049_001326"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9432430.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGTIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            764..1228
                     /locus_tag="VW049_001327"
     CDS             764..1228
                     /locus_tag="VW049_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9432431.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAVREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLGDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1241..2509
                     /locus_tag="VW049_001328"
     CDS             1241..2509
                     /locus_tag="VW049_001328"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9432432.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2523..2783
                     /locus_tag="VW049_001329"
     CDS             2523..2783
                     /locus_tag="VW049_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432433.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2794..4155
                     /gene="pdrM"
                     /locus_tag="VW049_001330"
     CDS             2794..4155
                     /gene="pdrM"
                     /locus_tag="VW049_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9432434.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4309..5277
                     /locus_tag="VW049_001331"
     CDS             4309..5277
                     /locus_tag="VW049_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9432435.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5293..6285
                     /locus_tag="VW049_001332"
     CDS             5293..6285
                     /locus_tag="VW049_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9432436.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNSVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6503..7546
                     /locus_tag="VW049_001333"
     CDS             6503..7546
                     /locus_tag="VW049_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9432437.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     RVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPCINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7610..9295
                     /gene="lpdA"
                     /locus_tag="VW049_001334"
     CDS             7610..9295
                     /gene="lpdA"
                     /locus_tag="VW049_001334"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9432438.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENISHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9359..10348
                     /locus_tag="VW049_001335"
     CDS             9359..10348
                     /locus_tag="VW049_001335"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9432439.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFVGISREEIAEAVVG"
     gene            complement(10421..11491)
                     /gene="xerS"
                     /locus_tag="VW049_001336"
     CDS             complement(10421..11491)
                     /gene="xerS"
                     /locus_tag="VW049_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9432440.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETAGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3385 a         2174 c         2612 g         3343 t
ORIGIN      
        1 atatatagtc gatttattaa atctttagat aaactgtaaa aagtggtttc catagccact
       61 ttttgctata atagaggcag taaaaatgaa ggagttggct atggaacact cgtcttggca
      121 cgctttgatt aaggcgcaat tacctgaggg ttatttcggg aaaatcaatc agtttatgga
      181 gcaggtctat tctcagggga ctatttatcc acccaaggaa aaggtttttc aggctctctt
      241 gacaacactg cttgaagaag ttaaggtggt aattctaggg caagacccct accacggacc
      301 aggtcaagcg cagggcttga gtttttctgt acctgactct atcccagctc caccatcctt
      361 gcaaaatatc ttgaaagaat tgtcagatga tatcggagtt aagaaatctc atgatttgac
      421 agcttgggct gagcaaggag tcttgcttct taatgcttgt ttgactgttc ctgctggaca
      481 ggccaatggt catgctggtc aaatctggga gccttttact gatgctgtga ttcaggtggt
      541 caatcatcta gatagaccag tcgtttttgt actctgggga gcttatgcac gtaagaagaa
      601 ggcattagtt accaatcctc atcacttgat tatcgaatca gcccatccaa gtcctttgtc
      661 ggtttataga ggattttggg gttccaagcc cttttccaag gccaatgcat tcttaaaaga
      721 gacaggacaa gagccaatcg attggcttag ataaggagaa aatatgcctc agttagcgac
      781 gatttgctac attgataatg ggaaagaact gctcatgctc catcgtaata agaaacccaa
      841 tgatgtccat gaagggaaat ggattggtgt gggtggtaag ctagagagag gagaaacgcc
      901 ccaggaatgc gcggtgcgtg aaatccttga agaaacaggg ctcaaagcca agccagttct
      961 aaaaggtgtc atcacttttc ctgaatttac accagattta gactggtaca cctatgtttt
     1021 taaggtgacg gagtttgagg gcgacttgat tgactgcaat gaggggacgc tagaatgggt
     1081 tccctatgat gaggttttga gcaagccgac ttgggaaggt gaccacacct ttgttgagtg
     1141 gcttttaggg gataaaccct tcttttcagc caagtttgtt tatgatgggg ataaattgtt
     1201 ggatacccaa gttgatttct atgaataaag gagaaagcag atgctactaa tcaaaaatgg
     1261 tcgtgtaatg gatcctaagt ctggtttgga tcaagtttgt gatgtcttag ttcaagatgg
     1321 gaaaattatc aaaattgcgc ctgagatcac ggaagaagga gcagaaacga ttgatgctac
     1381 tggtcttgtg gttgctcctg gtttggtcga tattcatgtg catttccgtg aacctggtca
     1441 aacacataaa gaagacattc atactggtgc cctggcagcc gctgcaggtg gttttactac
     1501 tgtcgtcatg atggctaata ccagtccaac catttcagac gtggagactt tgcaagcagt
     1561 tctccagtca gctgccaaag agaagattaa tgtcaagaca gttgcgacca ttactaaaaa
     1621 ctttaatggt aaaaacttga ctgactttaa ggcgctctta gaagctggtg cggttggttt
     1681 ctctgatgac ggtattccgc ttgagagcag taagattgtc aaggaagcca tggaggaagc
     1741 caaaaaactc aataccttta tcagccttca tgaggaagat ccaggtttga acggtgttct
     1801 tggctttaat gaaaatattg ctagagaaca tttccatatc tgcggtgcta ctggggtggc
     1861 tgagtacgct atgatggcgc gtgatgtcat gattgcctat gcaactaaag cccatgttca
     1921 catccagcat ttgtctaagg aagaaagtgt taaagtagtg gagtttgctc aggggttagg
     1981 tgcagaagtc acagcagaag tagcgccaca gcatttctct aagacagaag cacttctttt
     2041 aacacaaggt agcaatgcta agatgaatcc accgcttcgt ttggaatcag accgtcgtgc
     2101 cgttatcgaa ggtctcaaat caggtgtcat cacagttatt gcgactgacc acgcgcctca
     2161 tcatgtagat gaaaaaaatg ttgaggatat taccaaagcg ccatctggta tgactggctt
     2221 agaaacatcc ctgtctctcg gcttgaccta tttagtagaa gctggtgagt tgagcttgat
     2281 ggaattactt gaaaaaatga catacaaccc agccaagctt tacaactttg aagcaggtta
     2341 cttggctgag aatggtccag cagatatcac tatttttgat gccaaggctg accgccttgt
     2401 ggactcccat tttgcttcca aagcagctaa ttcaccattc atcggtgaaa ccttaaaagg
     2461 gcaggttaaa tataccatct gtaagggaca aatcgtctat caagcttgat aggagttaga
     2521 atatgaatta ttttcgaaaa cgtagggaga gacaagcgaa atctaatagt ggaatttact
     2581 gtcccgcagc taatcgttcc aaaattcaat atgaaactaa agaaaaagct gaccatgcgg
     2641 ttcaatatga taacggaggt ttagttagaa gctattattg tcgtacatgt gcgtgttggc
     2701 atactacttc aaaatctaat aaacctccgc taagtttgaa aaactatggt ctgaaactct
     2761 ttggtttaga taaatcagaa tagaaagaga agtatgtata agacaaagtg tttacgagag
     2821 aagttagtat tatttttaaa aattttcttc ccaatcctga tctaccaatt tgccaattat
     2881 tctgcctctt ttgttgatac tgcaatgaca ggtcaataca acactatgga cttggctggt
     2941 gtatctatgg caaccagtat ctggaatcct ttctttacat ttctaacagg gattgtgtca
     3001 gccttggtgc ctatcattgg tcaccatctt ggtcgaggca aaaaggaaga agttgcgtct
     3061 gatttttacc aatttattta tttggccttg ggcctatctg tggtcttgct ggggatggta
     3121 cttttcttgg caccaacaat cttgaatcat attgggttag aagcagcagt agcggcagta
     3181 gcggttcgct atctttggtt tttatctatc gggattatcc ccttgttgct ctttagcgtc
     3241 attcgttcct tgctggattc gctgggcttg accaaactgt ccatgtacct catgcttttg
     3301 ttactccctc tcaatagcgg atttaactat ctcttgattt acggtgcctt tggtgttcca
     3361 gaactgggag gggctggtgc tggtttagga acatccttgg cctactgggt cttgcttggg
     3421 atttctgttc tggttttatt taaacaggag aagctcaaag ccttacacct tgagaaacga
     3481 attccactta atatggataa cattaaggaa ggagttcgtt taggtctgcc tattggggga
     3541 actgttttcg cggaagtggc tatcttttca gtggttggct tgattatggc taagttttcg
     3601 tccttgatta tagctagtca ccagtcagct atgaactttt caagtcttat gtacgccttt
     3661 cctatgagta tctcatcggc tatggctatt gtcgtttcct atgaagtggg agccaagcga
     3721 tttgatgatg cgaaaaccta tattggtcta ggaagatgga ctgccctcat ttttgcggcc
     3781 ttcaccttaa ccttccttta catttttagg ggaaatgtgg ccagtcttta tggtaacgac
     3841 ccaaaattta tcgatttgac agtgcgtttt ttaacttata gtcttttctt ccagttagca
     3901 gatacctttg cggcgccgct tcagggaatt ttgcgggggt ataaggatac agttattcct
     3961 ttttaccttg gtttgcttgg ttattggggc gtagcaatcc ctgtggctac gctatttgat
     4021 tctctaacag attttggagc ttattcttac tggatcggct tgattattag tttgattgtg
     4081 agtggggcgc tctaccgttg gcgtttaact gtgattatga agagatttga atctttagca
     4141 aaatccaaac gctaaaaagt ttgtgaaaaa atattcttga taagaaaatc ccttgttttc
     4201 acggggtttt cttttttatt ttgtgaaatt tcttttagaa aaactttgac agtatttgcc
     4261 aaaaacggta aaatagtaag gtaagaagaa agttagaaag gcaagaagat gtcaacttta
     4321 gataaaaatc ttttgctaga aatgttccgt aagatggaag aaatccgtcg catggactta
     4381 aaaattgcac aattagtaaa gaaagggaaa gtgccaggaa tgacgcactt ttctgttggt
     4441 gaagaggcag ctaacgtggg ggctatgtta gctctcaatc cagatgatct gattacctca
     4501 aaccaccgtg gacacgggca agctattgct aaagggattg acctcaacgg aatgatggct
     4561 gaaatccttg gtaaatacac tggaacctgt aaagggaaag gtggatctat gcatatcgct
     4621 gaccttgatg ctgggaacct tggtgccaat ggtatcgtag gtggtggtat ggggatcgct
     4681 gtcggtgcag ccctcagtca gcaaatgcaa aataccggta aaatcgttgt ttgcttcttt
     4741 ggagatggtg cgaccaatga aggtgttttc cacgaagcag tgaacatggc ttctatctgg
     4801 aacctgccag tcattttcta ttgcattaac aacggttacg gtatctctgc ggatatcaag
     4861 aaaatgacca atatagaaca tatccatcaa cgtagcgccg cttatggaat tcctggaatg
     4921 ttcatcgaag acggtaacaa tgtcatcgat gtctatgaag gatttcagaa agctgtagac
     4981 catgttcgca gtggcaatgg tccagtcttg attgaaagtg taacttatcg ctggctcggt
     5041 cactcatcat ctgaccctgg taaatatcgt acgcgtgaag aagtggaatt gtggaaacaa
     5101 aaagatccaa tcgaaaacct ccgcaattac cttattgaaa ataacattgc aagtgccgaa
     5161 gaattggaag aaatccaagc gcaagtaaag gaagcagtag aagcttctgt taaatttgca
     5221 gaggaaagcc cattcccacc gcttgaatca gcctttgaag atatttacgc agactaagga
     5281 gaaagagata aaatggaaac aaaaacaatg tccttccgtg acaccattat ccttgctatg
     5341 tctgaggaaa tgcgtcgcga tgaaaatgtg ttcttgatgg gagaagacgt cggtgtcttc
     5401 ggaggagact tcggtacttc tgttggaatg cttgaagaat ttggtccaga acgcgtccgt
     5461 gactgtccga tttctgaagc tgccatctct ggagcagcag caggagcagc catgacagga
     5521 cttcgtccaa tcgtcgatat gaccttcatg gacttctctg ttattgccat ggacaatatc
     5581 gtcaaccaag ctgctaaaac acgttacatg tttggtggta aaggtcaggt tccaatgact
     5641 gttcgttgtg cagctggtaa cggagttggc tctgccgccc agcactcaca atctctagag
     5701 tcttggttta ctcacattcc aggacttaag gttgtggcac ctggaacacc tgcggacatg
     5761 aaaggcctgc ttaagtcttc tatccgtgat aacaactcag ttattatact tgagtacaag
     5821 tcagaattta accaaaaagg ggaagtgcca gttgatccag actacacaat tccacttggg
     5881 gttggtgaaa tcaaacgcca aggaacggat gtaacagttg ttacttatgg aaaaatgctt
     5941 cgccgtgtgg ttcaagctgc tgaagaatta gcagaagaag gaatttcagt tgaaattgtt
     6001 gacccacgta cccttgttcc gcttgataag gatatcatca taaactcagt gaagaaaact
     6061 ggtaaggttg ttctggtcaa cgatgcccac aaaacaagcg gctatatcgg tgaaatttca
     6121 gctattattt cagaatcaga agcatttgac tatctagatg caccaatccg ccgttgtgca
     6181 ggagaagatg tgccgatgcc ttatgcacaa aacctagaaa atgcaatgat tccaacagtt
     6241 gaaagtatca aagatgcaat ccgaaaaact tataacaaag aatagagttg cataagataa
     6301 gttatgtaaa taaaataaag ctgacgagaa actttgtatc ttttaggtgc agagctcttc
     6361 gtcattaata tcttagatta gagcacgggc taaaagctcg gaaaaaagat aaatctcctt
     6421 ggcttcatcg aagccatcgt cgatttccta tttttcagtc gcttttgacg ccctttgtat
     6481 cataaataga attggagagg tcatggctga tgacaagcta agagcgactc ctgcagctag
     6541 aaagttagca gatgatttag ggataaacct ctacgacgtt tctggctcag gtgcaaacgg
     6601 tcgtgtccac aaagaagacg tggaaactta taaagacaca aacgtggttc gcatttcgcc
     6661 acttgcaaaa cgaattgccc tcgaacataa cattgcttgg caggaaatcc aaggaaccgg
     6721 tcatcgtggt aaaatcatga agaaggatgt tttggccctg cttcctgaaa atatcgaaaa
     6781 cgatagcatc aaatctcctg ctcagattga aaaagtggaa gaagtccctg ataacgtaac
     6841 accatatggt gaaatcgagc gtattccaat gacaccaatg cgtaaggtta ttgcccaacg
     6901 catggttgaa tcttacttga ctgcgccaac cttcaccctc aactatgaag ttgatatgac
     6961 tgaaatgttg gctcttcgta agagggttct tgagccaatc atggaagcaa ctgggaagaa
     7021 gactactgta acagaccttc tttcacttgc agttgttaag actcttatga aacatccatg
     7081 catcaacgct tcattgacag aagatggcaa gactattatc actcacaact atgtcaatct
     7141 tgccatggca gttgggatgg ataatggatt gatgacacct gttgtttaca atgctgagaa
     7201 gatgagtctt tcagaactgg ttgtagcctt taaggatgtt attggccgta ccttggatgg
     7261 taaattggct ccaagtgagt tacaaaattc aacattcaca atcagtaatt tgggaatgtt
     7321 tggtgttcag tcctttggtc cgattattaa ccaacccaac tcagctatcc ttggtgtcag
     7381 ttcgacaatc gagaagccag ttgtcgtcaa tggtgaaatt gtgattcgcc caatcatgag
     7441 tttaggatta accattgacc accgtgtcgt agatggtatg gctggtgcta agtttatgaa
     7501 ggacttgaaa gagttgattg aaacgccaat ctcaatgtta atttaagagc aagagtattc
     7561 atttttaaga tatagataaa ggaaggaaga catggcctta gaagtaatta tgccaaaagc
     7621 cggcgtggat atgacagaag gacaaatcgt ccaatggaat aaaaaagtcg gagaatttgt
     7681 aaaagaagga gaaatccttt tggaaatcat gactgataaa gtcagcatgg aattggaagc
     7741 cgaagaagat gggtacttga ttgccattct caaaggagat ggtgaaactg tccctgtaac
     7801 ggaagttatc ggttaccttg gtgaagaaag ggaaaacatc ccaacagctg gagcagcatc
     7861 gccagaagct agccctgtac ctgtagcaag tacctcaaac gatgatggta agagcgatga
     7921 tgcttttgat atcgttgtga ttggtggagg tcctgctggt tatgttgcag ccattaaagc
     7981 tgcccaactc ggcggtaagg ttgcccttgt tgagaaatct gaacttggtg gaacctgctt
     8041 aaaccgtgga tgtattccaa ccaagaccta ccttcataac gctgaaatta ttgaaaatat
     8101 cagtcatgct gcaaatcgtg gtatcgtgat tgaaaatcct aacttcactg ttgatatgga
     8161 aaaactttta gaaactaaat ctaaagttgt taatactctt gttggtggtg ttgcaggact
     8221 tcttcgtagc tacggagtta ctgttcataa aggaattggt acaatcacta aagacaagaa
     8281 cgtcttggta aatggttctg aattgcttga aaccaagaaa atcattcttg ctggtggttc
     8341 aaaagtcagc aagatcaacg tccctggtat ggaatctcca cttgtcatga ctagtgatga
     8401 cattcttgaa atgaacgaag tgccagaaag ccttgttatc atcggtggtg gagttgtcgg
     8461 tatcgaactc ggtcaggcct tcatgacatt tggttcaaaa gtgactgtta tcgaaatgat
     8521 ggaccgtata gttccagcta tggatgcgga agtttctaag aaccttcgct tgatccttga
     8581 gcgtaaagga atgaccatct tgactggtac taaactgcaa gaaatcattg aggaaaatgg
     8641 tcaacttcgt atcaaggttg aaggaaaaga cgatatcatc gcaagcaaag ctcttctttc
     8701 aattggtcgt atgccagacc ttgaaggtat tggagaggtt gagtttgaat tggatcgtgg
     8761 ttgtatcaag gtcaacgaat acatggaaac ttcagttcca ggcatttatg caccaggtga
     8821 catcaacggt actaagatgt tggctcacgc agctttccgc atgggtgaag tttccgctga
     8881 aaatgccctt aaaggaaatc atgcagttgc caaattgaat ttgactcctg cagccatcta
     8941 cactctccct gaagtagcag cagtaggttt gacagaagaa caagcccgtg agaaatacga
     9001 tgttgccatc ggtaagttta actttgctgc taacggtcgt gctattgcat ctgacgcagc
     9061 tcaaggtttc gtaaaagtta tcgctgataa gaaatatgga gaaatccttg gtgtacacat
     9121 tattggtcct gcagccgcag aattaataaa cgaagcatca agcatcatcg aaatggaaat
     9181 cactgttgag gaaatgctga agaccatcca cgggcaccca acttactctg aagtgatgta
     9241 cgaagcgttt gcagatgttc taggaatggc catccattca cctaagaaaa aataaaacga
     9301 agatagatta gactggaagg ttatttccag tctctatcat ataaagtaaa gggatatcat
     9361 gaaatacatt atcaatcatt caaatgacac tgcttttaat attgccttgg aagaatacgc
     9421 ctttaaacac cttttggatg aggatcaaat cttcctactt tggattaaca agccatctat
     9481 cattgttggt cgtcaccaga acactatcga agaaatcaac cgtgattatg ttcgcgaaaa
     9541 tggtattgag gtagttcgcc gtattagtgg tggtggagct gtttaccacg atttaaataa
     9601 cctcaactac acgatcatct ccaaagaaga tgaaaataag gcatttgact tcaagagctt
     9661 ctcaactccg gttatcaata ccctggctca actcggggtt aaagctgagt tcacaggtcg
     9721 taatgacctt gagattgatg gtaagaagtt ctgtggcaat gcccaagcct atatcaacgg
     9781 ccgtatcatg caccacggtt gcttgctctt tgacgttgat ttgtcagtcc tcgcaaatgc
     9841 ccttaaggtt tcaaaagata aatttgaatc aaaaggtgtg aaatccgtcc gtgcccgtgt
     9901 aactaatatt atcaatgaat taccaaaaaa aatcacagtc gaaaaattcc gtgatttact
     9961 cttggaatac atgaaaaaag agtacccaga gatgactgaa tacgtctttt cagaagaaga
    10021 attggccgaa atcaatcaca tcaaggatac taagtttgga acttgggact ggaactatgg
    10081 taaatcacct gaatttaacg tccgtcgtgg aaccaaattc actagtggta aggttgaagt
    10141 ctttgctaac gttactgaat caaaaatcca agacatcaag atttatggtg acttctttgg
    10201 tattgaagac gttgctgcag tagaagatgt ccttcgtggg gtgaaatacg aacgcgaaga
    10261 tgttcttaag gcgctaaaaa ccattgatat cacacgctac ttcgttggta ttagccgtga
    10321 agaaattgct gaagcggtag ttggataaat caaaaagaag ttggccaact aaccaacttc
    10381 ttttttcgat atttatttac ataatttaaa atacgtaaaa tcataaacta tccagagcat
    10441 tcttttgttc atcactaaca atatgggtat agaggtcagt gacttgtgtg ctagcatgtc
    10501 ctagttggtg actgactaaa acttgtgatt tagtcgcatc atagagccta gttgctagtg
    10561 tatggcgcag tttatggggt gttacacgca ctttaaaatc ctctgagtat ttagcaacca
    10621 ttttctcaac gctagaagca tcgatacgat taggaacacc tctgtagaga gttaaaaaaa
    10681 gggctgtatc tgttttttcc gttttatagc gttgattccg aatggccaga taattctcta
    10741 aataaggttt agcaaaagca gcgacattga ctgagtcacg tttgccacct tttcgagtaa
    10801 catcaataac catcattttt agattgagat ctcttagatc tagattaaca gcttcagata
    10861 agcgaacacc agatgccaag agaagggcaa taatggctaa atctcgttct ttatttttgt
    10921 tgaatgatga gagagctcga tttgaaagct gttgtgggtg ctcttgatcg atataagtta
    10981 gaaaacctgc tgtttcatca cctagaaaga gtttttgctt gatattttca gctctggcag
    11041 caagggtttc tttctttttc ttggttgaaa cttttttcat tacattacga tagaaataag
    11101 gttccccctg atcgttttca acctcctcgg ttagatactt gtaaagacta gaaagtgctg
    11161 ataaggttcg attgatagtt gtctgtgaaa caccttgttt tgttgtatta gcattcagca
    11221 agggacgttc acgtagataa aggataaagg attccatgtc tttcttagac atattttcca
    11281 aaactgataa aggaatatcg gatattttat cagcgtttga aataccagac tccaaaaccc
    11341 agctgaaaaa tcggtcatat tccttaagat attcgtacag ggttgtaaaa ctgtagggca
    11401 cagccagctt agattggtag tattccagaa cataccaggg catgagttgt tttagtttgt
    11461 cgattcgttc cagtaaaatc tcacgtttca tgtattttct ccataattaa gtat
//
LOCUS       DAVKGC010000059        11473 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKGC010000059 DAVKGC010000000
VERSION     DAVKGC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.059"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(56..286)
                     /locus_tag="VW049_001337"
     CDS             complement(56..286)
                     /locus_tag="VW049_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9432441.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VW049_001338"
     CDS             complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VW049_001338"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9432442.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(1113..2036)
                     /locus_tag="VW049_001339"
     CDS             complement(1113..2036)
                     /locus_tag="VW049_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9432443.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(2033..2722)
                     /locus_tag="VW049_001340"
     CDS             complement(2033..2722)
                     /locus_tag="VW049_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9432444.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(2787..3491)
                     /locus_tag="VW049_001341"
     CDS             complement(2787..3491)
                     /locus_tag="VW049_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9432445.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(3547..4569)
                     /locus_tag="VW049_001342"
     CDS             complement(3547..4569)
                     /locus_tag="VW049_001342"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9432446.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(4699..7170)
                     /gene="parC"
                     /locus_tag="VW049_001343"
     CDS             complement(4699..7170)
                     /gene="parC"
                     /locus_tag="VW049_001343"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9432447.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVSFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEAILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(7590..9533)
                     /gene="parE"
                     /locus_tag="VW049_001344"
     CDS             complement(7590..9533)
                     /gene="parE"
                     /locus_tag="VW049_001344"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9432448.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            9669..10310
                     /gene="plsY"
                     /locus_tag="VW049_001345"
     CDS             9669..10310
                     /gene="plsY"
                     /locus_tag="VW049_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9432449.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDFLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(10353..11473)
                     /locus_tag="VW049_001346"
                     /pseudo
     CDS             complement(10353..11473)
                     /locus_tag="VW049_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3248 a         2561 c         2035 g         3629 t
ORIGIN      
        1 ctctcctaca atcaacatgg actattatat catgaaaatt tcaaaaaaac aagacttatt
       61 ttgctaaatt ataattttca atcaaaattt ccacagcttt ttccaatttt ttataaaaac
      121 tatcgacatt cccattcttt ataatggcaa attgtccgtc taattcggca atttctccga
      181 taactttttt accgatagtc aatgtataac cttcaaaact gtctttacca acattaactt
      241 tggcatccgc tacttgaatt tcgatttttt tatctttctt gctcatttca tacctctctt
      301 cactttttct attttacaag aaaattctaa aactagcaag aaaaaactct atatcaggct
      361 tctctgatat agagttttat gtaaacaacc tcaattgatt tagcatagtt aatagttagt
      421 aaacaagcat aattgactat cctttttagg ttatcagtga gtctcaccgc ctactaactg
      481 caaatcctta tctttgtagt ctacaatagc ttcaatagct acctctatgc ctcttacaat
      541 atcacataga ctcattcctg cagtattcgg cttatttact acttgttcca tcatatatgg
      601 aatatgcatg aaacctgctc tcatattagg gaatttttta tccactaaat aaagagcttg
      661 gtacatcaaa tgattgcaaa caaaggttcc tgcactattg gatacaactg ccggaagtcc
      721 ctgttttttg atagcttgta ccatcgcttt gataggtaaa ctactaaaat aggccgatgc
      781 tccatcaata cgaatcggtg tatcaattgg ttgattgcct tcgttatcag gtatgcgagc
      841 atcatcttga ttaatagcca ctcgttcagg tgttaagccg gtcctgccgc ctgcttgtcc
      901 aatacaaagt acagcatctg gttgatatcg taatatttct gcctctaaaa cttctgacga
      961 cttataaaaa accgttggaa tttctaccca gcgaacttca gccccattaa tctcagatgg
     1021 taataatttt acagcctcca aagctggatt aatcttttca cctccaaaag gattaaaacc
     1081 tgtaaccaat attttcattt tattttcctt tactaaaatg cgagaaagta cattaagaat
     1141 atgtgaataa caatcattac tagagcaaca cctgcttgag cctttataac gccattctga
     1201 tctttcatat ccatcaatgc tgctggtaga gcgttaaaat tagcagccat tggggtcaat
     1261 aaggtcccac aataacctgc tgtcatggca agagcaccag ccacaattgg attagctccc
     1321 agagcaaata caaagggaac tccaacacct gctgtaataa cggtgaatgc tgcaaaagca
     1381 tttcccataa tcattgtgaa tagaaccatt ccaagaacat aggccaaaac tcctataaag
     1441 cgactatctg aaggaacaat accgctaatc agatgagaga taacatcacc aacacctgct
     1501 acagtaaaaa tagcccccaa agcccctaat aattgaggaa caatcccact tgttgaaact
     1561 tgctgagtca ttcgattatt ttctgataac agactcttag ggtgactatt ggtaatcaca
     1621 agaacagaaa ttgtagcaaa caaggcggca aggctaatcg aaatcttgct aaattctgga
     1681 atcatttgcg ctaagaccaa cgcaagtatt gccatcagca taactggaat aaaaatttta
     1741 tttttcaacc tgttagattc aatattggct ttcatttcat ctaaggatgg caaggttccg
     1801 atacggactt gcttaaacaa tgttaacagc gataatagga ttacaataat accaatactc
     1861 atatttggca tataggaacc acctataaac gtaatagaca atagagtcca aaatgcagat
     1921 gtcccaagtc gaactgggtt tgttttatct ttataactac aataggctgt atggagaaat
     1981 tgacaaccaa tcacaatata ggtcaactct aatagttgct ttgccaactc tgtcattttt
     2041 gttctcctcc cctagtcttt tttgatatca attttttatc aaataaataa ttgtaaatcc
     2101 ccactacaat aagtgttata acagcaacaa taatagatgt agaagcaatc cctgcataat
     2161 tgctttcata gcctaactga tctaatgttc cccctatcaa gaggactccc ccagcaccta
     2221 caaacgtatt ttgagcaaag aaatttccaa aattttcatt cgctgccgca cgcgctttta
     2281 ttgtctcatc ttcaacctct gttaactttc tacctaattg agactctgca gctgcttctc
     2341 ccataggctg aaccaaaggt ctgacaaact gagggtgtcc tcctagacga attgaaaaga
     2401 aaccagctaa ctctcgaata aagaaataaa ctgtatagaa gtttccaact gtcagacctt
     2461 taatctttcg aatcaaatcg attgatcgtt gcttgagtcc aaaggtttct gacagcccca
     2521 caagaggcaa ggtaaccata aaaatcgtga gcactcgctg attgctaaat tcttttccca
     2581 aaatctccaa aaattcaacg agagaaacac ctgaaactaa agctgtaacc aaaccagcta
     2641 agactactgt tgcaattgta tcaaatttta aaataaaacc cacaacaatg attgctattc
     2701 ctattaatct aatccactcc atatcaaact cctttatatt caaaatgaca gtattttaaa
     2761 aattttatca agatcaatac cattccttat ttaatgtatt tttctagttc tttttggtat
     2821 ttgctgttgg attccaattt ttctttttgc cattttttaa aaacctcgtt atattctttt
     2881 gttgtaacaa tatctttttg caatttcatt cctttaaaga tatatggttc ccccttaata
     2941 ccaacttgtg agtatggttt tgagaatggt actacgttac ttacaactgg aaaaccacca
     3001 gatgaagctg ttggcatcaa taatgaacta tctgtcgacc aagcttgagc tttggcatat
     3061 ttttcatatc tcttctctag gtcagtggtc tcagaaacag catcttctaa caatttctta
     3121 tatttatcca aaccaggttt agctacaaca tccttatctt ttcctttcgt aatgccaagg
     3181 tgtttcatgg cagaaccaga ttttggatcc ataatattca agtaagacgc tggatcttga
     3241 tagcttggag cccatcctgt actgttcaaa tcatagtctt tttgagaagg agcaacattg
     3301 ccgtatttat cattttccat caaaccatca ataacatttc caataacgtc tgtcctcgat
     3361 gttcgagtcg ctatactgta gcccaatgat gctggatcta ctgcatagac ataagaaaat
     3421 gttgtcggtg catctgcttc tttatcagtt tttccacaag ccactaaaat agctgacgtg
     3481 ctcaggacca ctcctgctgt taagagccac tttttctatt tcataaaaaa tctcctttgg
     3541 tttattttaa tctactttta caatccaacc ttctggcgct tcaatatcgc caaactgaat
     3601 acccgtcaat tcattatata atttacgcgt cacaggacct acttctgttt cactatagaa
     3661 tacatggaaa tcatcaccat gttgaatacc tccaattgga gaaataaccg ctgctgtacc
     3721 acaggcacct gcctctacaa aacggtcaag attatcaatt ggaacatcac cctcaatagg
     3781 agttaatccc aagcgatgtt ctgccaaata aagcaaggaa tacttggtaa tagatggcaa
     3841 gatagatgga ctcaatggtg ttacaaattc attatcagct gtaattccaa agaaattagc
     3901 tgatccgact tcttcaatct ttgtatgagt tgatgggtcc agatagataa catctgagaa
     3961 atggcgtgac ttggccattt ttcctggtaa gagacttgca gcatagtttc caccaacctt
     4021 agccgcacct gtaccatttg gtgctgcacg gtcgtactca tcctgaatca agaagttggt
     4081 tgggaccaaa ccacctttaa agtaatttcc aactggcata gcaaagatgg tgaaaatgta
     4141 ctcttctgcc ggttttaccc cgataatatc tccgacacca atcaaaagag ggcgaagata
     4201 taaggttcca cctattccgt atggtggtac gtattcttca ttcgcacgga caactgcttt
     4261 acaagcttct acaaacatgt ctgtcggaac ttgtggcatc aagagacggt cacatgtacg
     4321 ttgcaggcgt ttagcatttt catcaggacg gaacagttga acactgccat ccttagtacg
     4381 ataagctttc aaaccttcaa atgcttgttg tccatagtga agacttggag aagactctga
     4441 aatatgcaaa gttgcatcct ctgtaagctc tccttgatcc cattgtccat ttttgaaatg
     4501 agcaagatag cgataaggta atttcatata ggaaaaaccg aggttttccc aatcaatcgt
     4561 tactgtcata ttccactcct tattctaaaa acctatttct tatattctac actatttttc
     4621 taaaatagca agtatatttt gtaattttca gaaaatttct ccaataaaaa ccatcttaga
     4681 actgattctt catttcactt atttatcttc agtaactact tcctgaagat aagcgtcaaa
     4741 aacttcttca tctgaaatcg tgtcagaaat gaagcttcca ttgctagtgc gttctgacaa
     4801 gttcaagtct tgcaatcggc tttcatagat tgttccttta ttggattgga caagcagagt
     4861 ttggtcgttc acatccactt ccgtactgaa gaaatcgcca acaaatcctt gctctgcaac
     4921 tgctcctgcc aagaagacac gatgcggttt gtttttcaac tcacgcaaga cttgtaatcc
     4981 tcgtttggca cggctggttg ctagaatttc ctcaatggaa acacgtttca agcttccacg
     5041 ctgggtcaag aggtagaagg acgaagtatt acagataaag ccagattgga ggacatcatc
     5101 ttctttcaaa ttcatagcct tgacacctgc tgccttagca ccgacaaccg gaacctcttc
     5161 gatattgaaa cgcagggcat aaccattttg actaaccaag acaacatcat ctagtttaat
     5221 cggagccact gctacaatct gatctgtatc gtctttgagc ttagcatact tgacagactt
     5281 agatctatag gtccgccatg gagtgaattc ttttcgctct acccgtttga tttgaccaag
     5341 gcgagtcgct gcaaagtagg ttgtcgcatc gtcaaactga tccagtactt ccacataaag
     5401 gattgcttca ttcgtttcaa agtttgtgat ggtttggctc agatgctctc cgatgtcctt
     5461 ccaacgaata tctgccaact catggattgg tctgtagatg acatttccaa gacttgtgaa
     5521 catcaagagg tgctgggttg tcttggcaga ttgaacaaaa atcaaacggt catcatcacg
     5581 cttgccaatt tcttccaagg tggaagccgc aaaggaacgt ggactggtac gcttgatgta
     5641 acctgccttg gtcacgctga cgtaggtatc ttcctcagcg ataagactag ctgtatcaat
     5701 ctcaattgct ttcgcagtgt cttctaaaga actcaaacga ggagttgcaa atttcttctt
     5761 gacctcacga agttctttct tcatgagatt gtacatagtc ctttcatcac caataatagc
     5821 cgccagcata gcaatcttct cacgaagctc tgcttcttct tcctgcaaga caaccacatc
     5881 ggtattggtc aaacggtaca gttgcaaagt tacgatagcc tcagcctgtt cttccgtaaa
     5941 atcatagcta actttgaggt tttccttggc gtccgcctta ttctcagaag cacggataag
     6001 agcaatgact tcatccaaaa tcgaaatcac acgaatcaaa ccttcgacga tatggagacg
     6061 tttctcagcc ttttctttgt caaagcgtga acgcgccaaa atcacttctc gacggtgagc
     6121 gatgtagcta gacaggattg gaacaatccc aacctgacga ggtgtgaaat tgtcaatcgc
     6181 caccatatta aagttgtagt tgatttgtag gtcggtgtac ttaaataagt agttgagaac
     6241 aagctcagta ttagcgtctt tcttaagttc gatagcgata cgaagaccat cacggtcaga
     6301 ctcatcacga acctcagcaa tcccagctac cttgttatta acacgaacat catcgatttt
     6361 cttgactaga ttggccttat tgatttcata aggaatctca gtaataacga tttgttcctt
     6421 accacctttt agcttttcaa tttcagtctt ggaacgaaca accacgcgcc ctttcccagt
     6481 ctcataagct ttcttgattt catcacgacc ctgaataata gcccctgtag ggaagtctgg
     6541 tccaggtaag aattccatga gtttatcaat ctttgcagtt gggtggtcaa tcatgtaaac
     6601 tgcagcatct atgacctcag ctaaattatg gggaggaatg tctgtggcat aaccagccga
     6661 aatcccagtc gaaccattga ccaagaggtt tggaaaggct gctggcaaga ccgttggttc
     6721 tttctccgta tcgtcaaagt tccatgcaaa agaaactgtc tttttctcga tatcctgaag
     6781 aaggtagcct gcaatttcag acaaacgtgc ctcagtataa cgcatagccg caggaggatc
     6841 tccgtccata gaaccgttat taccgtgcat ttcaactaga atctcacgat ttttccagtt
     6901 ctgtgacata cgaaccatgg catcatagat agaagaatcc ccgtgtgggt ggaaattccc
     6961 catgatgttc ccgactgact tggccgactt acggtagctc ttgtcaaaag tattgctatc
     7021 cttattcata gaataaagaa tacggcgctg aaccggcttc aacccatcac gaatatctgg
     7081 caaagcccgg tcttgaataa tgtacttgga gtagcgacca aagcgctctc ccatgatgtc
     7141 ctccagggac atgttttgaa tgttagacat aagatacaaa gcccataaaa taccaagtga
     7201 aaatagaaaa ttcttgaagt aagcaaactc acaagagaat ttatcttttt cacacagtat
     7261 ctagggcgtg ttcaactcct ttcaaagaat gtagagtagg tttttatgca gtaaaagata
     7321 ttttacggga attcctcccg tgttcagtta cgataagtaa ccaaactatc ctgtttgtat
     7381 ttttcaatat gaaaatctgg ttttccaaaa ttagtcttag tttgtgtctt agccgctccc
     7441 ttaagcgcct ctttgagata agcactcata gcagattctt cattaataat cctgcaattt
     7501 tttcaaacca agattttcaa actgcttttt cacatagtca ttcacatccg actctaattt
     7561 ccagtttact aacatattat tttctttcat taaaacactg tcgcttcttc tagcgtaaac
     7621 ttgacattat cttcaatcca tttacggcgt ggttctacct tatctcccat gagaacattg
     7681 acgcggcgtt cggcgcgcgc taaatcttca attgtgacac ggatgagggt acgtgtttct
     7741 gggttcatgg ttgtttccca gagctggtcc gcattcatct caccaagtcc tttgtatcgt
     7801 tggagggtag cgcctttacc gaactgttta cggagttctt ctagttctcc gtccgtccaa
     7861 gcgtaggcca cttcttcttt ctttccttta cctttggaca tcttgtaaag aggtgggagg
     7921 gcaatataga catgacctgc ctcgactagc ggacgcatgt aacggtagaa aaatgtcaag
     7981 agcaaggtct ggatatgggc accgtcggta tccgcatcgg tcatgataat gatcttatca
     8041 tagttggcat cttcaataga gaagtctgct ccaacacccg caccaatggt ataaatcatg
     8101 gtattgatct cttcattttt gaggatatcc gccatcttgg ccttggctgt attgataacc
     8161 ttaccacgaa gaggtagaat agcctggaac ttgcggtcac gaccttgttt ggcagaacca
     8221 ccggcagagt ccccctcaac tagatagagt tcattcttag caggattctt agattgggct
     8281 ggggtcaatt tcccagacaa caagccctta tctttcttgt ttttcttccc atttcggctc
     8341 tcatcacgcg ccttacgtgc tgcttcacga gcatcacggg ccttgatagc cttgcggatg
     8401 aggttagaag ctaattcccc attttccata aggaaaaagg tcaacttatc agccactatt
     8461 ccatccacaa ctgggcgagc tagggggctt cctagtttat ccttggtctg tccttcaaac
     8521 tgcaagtgtt cttcaggaac taagatagaa agaacggccg ctagtccctc acgatagtct
     8581 gaaccttcaa ggtttttatc tttttccttg agaagacctg ttttacgtgc atagtcattc
     8641 atgaccttgg taatggcaga cttgagtcct gtctcgtgcg ttccaccgtc cttggtgcga
     8701 acgttattga caaaggatag aatgttatct gagaatccgt cattgtactg gagggctact
     8761 tccacttgaa aaccattgtc ttccccttca aagtaaagaa ctggcgtcaa gatttcctta
     8821 tcttcgttga gataagaaac aaaatcttgt actccattct catagtggaa ctcaatcgct
     8881 tcatctgttc gcttgtccgt taaagacaag gtcacatttt tcaagagaaa ggctgattca
     8941 ttaaggcgct ctgaaatggt attgtacttg aaatctgtcg tagaaaatat agtcgcgtca
     9001 ggcataaaag taactttggt gcctgtttta gacttgggtg ctgtaccgat tttcttcaaa
     9061 gtcgtgacag gttttccacc attttcgaaa cgttgcttgt aaactgcgcc atcacgggta
     9121 atttcaactt ctaaccagct agaaagggcg ttaacaacgg aagaacccac tccgtgaagt
     9181 ccacctgatg tcttatagcc accttgaccg aatttccctc cggcatgaag aatggtaaag
     9241 ataacctcaa cagttggaat tcccatagcg tgcatacctg tcggcatccc acgtccatgg
     9301 tcttgaaccg ttagactacc gtctttattg atagttacat caatacgatc accaaaccca
     9361 gacaaggctt catcgactgc attatcaacg atttcccaaa ctaggtgatg aagaccagcg
     9421 ccatcggtcg atccaatata catccccgga cgttttcgga ccgcatccaa cccttctagc
     9481 acctgaatag catcatcatt ataattgtta atattgattt ccttttttga cacaaggaac
     9541 ctcctattcg ttcatcttta ctattctaca ggttttccaa ggattttgca aaatttttct
     9601 ttctccgatg tgacaatttc agcagagatt ctctgctttt ctttcccaat tcatgatata
     9661 ataggagtat gattacaata gttttattaa tcctagccta tctgctgggt tcgattccat
     9721 ctggtctctg gattggacaa gtattctttc aaatcaatct acgcgagcat ggttctggta
     9781 acactggaac gaccaacacc ttccgcattt taggtaagaa agctggtatg gcaacctttg
     9841 tgattgactt tttcaaagga accctagcaa cgctgcttcc gattattttt catctacaag
     9901 gcgtttctcc tctcatcttt ggacttttgg ctgttatcgg ccataccttc cctatctttg
     9961 caggatttaa aggtggtaag gctgtcgcaa ccagtgctgg agtgattttc ggatttgcgc
    10021 ctatcttctg tctctacctt gcgattatct tctttggagc tctctatctt ggcagtatga
    10081 tttcactgtc tagtgtcaca gcatcgattg cggctgttat cggggttctg ctctttccac
    10141 tttttggttt tatcctgagt aactatgact ttctcttcat cgctattatc ttagcacttg
    10201 ctagtttgat tatcattcgt cataaggaca atatagctcg tatcaaaaat aaaactgaaa
    10261 atttggtccc ttggggattg aacctaaccc atcaagatcc taaaaaataa aacgccagtt
    10321 ctgtactgac cccaaacagt tagacaatta atttatccga aggatttagt tctgtattgc
    10381 acagggctaa gtccttttag ttttatctta attcgtttat tgttgtagta atcaatatag
    10441 tctacaatgg cttgttccaa ttgcttaagc gactgaaacg acttctcata accgtaaaac
    10501 atttccgatt tcagaatccc aaagaaggac tccatcatac cattgtctgg gctgttaccc
    10561 ttacgtgaca tggatgcttg aattccctta ccctcaagaa actgatgata agaatcgtgt
    10621 tgatattgcc agccttggtc actatggaga atcgtattct cgtagtgctt ctctgtgaat
    10681 gcctgttcca acattgtttg tacttgttct aagttgggtg aagttgaaag attataggcg
    10741 ataatttcgc tgttaaaacc atctaaaact ggtgataagt aaagcttttg agtacttact
    10801 ggaatggcaa attcagtcac atctgtgtag cactgttcca ttgttttaga gccttcaaat
    10861 tggccttgaa tgagattctc tgctttctta ccaacgtctc ctttatgaga agaatatttt
    10921 cgtttctgtc gcattttagc ttgtaaattg agtactttca tcaagccttg aactctttta
    10981 tgatttacca gataagcacg atttcttagt tctaaataaa tccgacgata accataattt
    11041 cctttgtgtt cgataaagat ggattgaatt tcagctttaa gctcttggtc cttatctggt
    11101 ttgtctagct gtttcaagtg atagtagtag gtcgaacgag ctagtttaat ggcttttaga
    11161 agaatatcta acgaaaactc agtcattaat tcttgaacaa tttctgtctt tcttctttct
    11221 ctttttcctc cttcaatcgg agttctctta acttttttag gatggcattc tccgctctca
    11281 ggtactcatt ttctgcttga agatgttcta attctgtcct ctcttcaggt ctctttttgg
    11341 gcttacgtcc cattttaggt actctccctc ttgttttctc aacaatagta tacccgtttt
    11401 tttctgtatt gtgctagcca gttaagaagt atcgtacgac ttgggagacc gtattcaaga
    11461 gaaactctgt ctt
//
LOCUS       DAVKGC010000060        11116 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKGC010000060 DAVKGC010000000
VERSION     DAVKGC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.060"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            1..1012
                     /locus_tag="VW049_001347"
                     /pseudo
     CDS             1..1012
                     /locus_tag="VW049_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1140..2162)
                     /gene="cbpF"
                     /locus_tag="VW049_001348"
     CDS             complement(1140..2162)
                     /gene="cbpF"
                     /locus_tag="VW049_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HEU9432450.1"
                     /translation="MKLLKKMMQVVLATFFFGLLGTSTVFADDSVGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEISLRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSPAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLDPATGI
                     MQTGWQYLGNKWYYLHSSGAMATGWYKEGSTWYYLDAENGDMRTGWQNLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(2181..3002)
                     /gene="cbpG"
                     /locus_tag="VW049_001349"
     CDS             complement(2181..3002)
                     /gene="cbpG"
                     /locus_tag="VW049_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEU9432451.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKNAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSAVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNRAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(3083..3322)
                     /locus_tag="VW049_001350"
     CDS             complement(3083..3322)
                     /locus_tag="VW049_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432452.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3472..3738)
                     /locus_tag="VW049_001351"
                     /pseudo
     CDS             complement(3472..3738)
                     /locus_tag="VW049_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3763..4074)
                     /locus_tag="VW049_001352"
     CDS             complement(3763..4074)
                     /locus_tag="VW049_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9432453.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLQLA
                     S"
     gene            complement(3999..4240)
                     /locus_tag="VW049_001353"
                     /pseudo
     CDS             complement(3999..4240)
                     /locus_tag="VW049_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(4241..4873)
                     /locus_tag="VW049_001354"
     CDS             complement(4241..4873)
                     /locus_tag="VW049_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9432454.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(4887..5882)
                     /locus_tag="VW049_001355"
     CDS             complement(4887..5882)
                     /locus_tag="VW049_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9432455.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKIQANLKRLLAGQEVVQVVDQDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5879..6577)
                     /locus_tag="VW049_001356"
     CDS             complement(5879..6577)
                     /locus_tag="VW049_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9432456.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAVTILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6655..7665)
                     /locus_tag="VW049_001357"
     CDS             complement(6655..7665)
                     /locus_tag="VW049_001357"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9432457.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SSQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(7649..8656)
                     /locus_tag="VW049_001358"
     CDS             complement(7649..8656)
                     /locus_tag="VW049_001358"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9432458.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8643..9596)
                     /gene="mvaD"
                     /locus_tag="VW049_001359"
     CDS             complement(8643..9596)
                     /gene="mvaD"
                     /locus_tag="VW049_001359"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9432459.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(9578..10456)
                     /gene="mvk"
                     /locus_tag="VW049_001360"
     CDS             complement(9578..10456)
                     /gene="mvk"
                     /locus_tag="VW049_001360"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9432460.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10578..10907)
                     /locus_tag="VW049_001361"
     CDS             complement(10578..10907)
                     /locus_tag="VW049_001361"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9432461.1"
                     /translation="MTRKVYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         3322 a         2535 c         1972 g         3287 t
ORIGIN      
        1 ggaatagagt tcgtcaaaaa aggaaaaaat cgttactatt ctcctgattt aaaacaagaa
       61 atgattcata aagtctgaca tgaaggctgg actaaagata gagtttctct tgaatactgt
      121 ctcccaagtc gtacgatact tcttaactgg ctagcacaat acaggaaaaa cgggtatact
      181 attgttgaga aaacaagagg gagagtacct gagagcggag aatgccatcc taaaaaagtt
      241 aagagaactc cgattgaagg aggaaaaaga gaaagaagaa agacagaaat tattcaagaa
      301 ttaatgactg agttttcgtt agatattctt ctaaaagcca ttaaactagc tcgttcgacc
      361 tactactatc acttgaaaca gctagacaaa ccagataagg accaagagct taaagctgaa
      421 attcaatcca tttttatcaa acacaaggga aattatgctt atcgccgggt tcatttagaa
      481 ctaagaaatc gtggttatct ggtaaatcat aaaagagttc aaggcttgat gaaagtactc
      541 aatttacaag ctaaaatgcg acagaaacga aaatattctt ctcataaagg agacgttggc
      601 aagaaggcag agaatctcat tcaaggccaa tttgaaggct ctaaaacaat ggaacagtgc
      661 tacacagatg tgactgaatt ttccattcca gcaagtactc aaaagcttta cttatcacca
      721 gttttagatg gctttaacag cgaaattatc gcctataatc tttcaacttc acccaactta
      781 gaacaagtaa aaacaatgtt ggaacaggca ttcacagaga agcactatgg tggagtcctt
      841 ctttgggatt ctgaaatcgg aaatgtttta tggctatgag aaaacattta aatcacttaa
      901 tcaattggaa caagccatta tagactatat tgattattac aacaacaaga gaattaaggt
      961 aaaactaaaa ggacttagtc ctgtgcagta cagaactaaa tcctttggat aaattatttg
     1021 tctaactttt ggggggcagt acagaaaaac cttgatacta tgcgtttttt gattgtgaag
     1081 acttttatcc acctattata caaagttaag tatccatcac agtttacaat tagccttcat
     1141 tacttaaccc attcaccatt atagtttaag tagtaaccac ctactgtggt attaacagct
     1201 aaagcacctg aatcataggc atagtaccag ttaccattga cttggaacca gcctgttttc
     1261 atatctccat tacttgcatt tagatagtac caagtcgaac cttcctgata ccaaccagtt
     1321 gccatagctc ctgatgaacg gagatagtac catttgttcc caaggttttg ccagccagtt
     1381 ctcatatcac cattttcagc atctagatag taccaagttg agccttcctt ataccagcca
     1441 gttgccatag ctcctgacga atggaggtag taccacttat tacctagata ttgccaacct
     1501 gtttgcataa tgccagttgc tggatctagg tagtaccatg gagctgcttt tagcttctct
     1561 tcatcatagg taagagggta atccttaacc caaccacgtg ctagctctcc caccgtcaat
     1621 ctgttgatgc gcgaatcaaa gccaccatcc ttctgtaaat aataccaatg accctcttca
     1681 taaatccaac cagtttttaa agtatgataa ctacgctgat cttcaaaata atagactcgt
     1741 ttctctgctg ggctatcata ttcttcccca tgatgtttgt tggtattcgt agcagttttt
     1801 gcttctaaaa cttgcttgcc aacaaattct tgtaatacac catcttgacc aaaataaaac
     1861 caatctggtc taagagaaat ctctattctt ggagaaggac caatcgtaac ccccttgtgt
     1921 ggagcaggta tatattgcca gccgacaacc atctctccgg ataaaggatc aaaataatag
     1981 tacttaccat caatcactcg ccagtaggtt tcttttagat cccccttttt gtagtaggtt
     2041 ctaccatttt cttggacaaa ctgccatcct acagaatcat ctgcaaatac tgtactggtc
     2101 cctagcaaac cgaagaaaaa tgtggctagt acgacttgca tcattttttt caaaagcttc
     2161 attttatata tcctccaaca ttaaatccac tcaccagatg aagcgaagtt ataaacttta
     2221 ccatcgatag tttggctacc tgtaaccatt gctctatttg aattgagata ataccatttt
     2281 ccatttactt tttgccaatc tgtaagcatc ttaccggaac tgtctaaata ataccaagta
     2341 tcatttatct cctgccaacc cgtttgttta tcatgttgtc tataatagta ccaactacca
     2401 tttattttct tccatccttc aagagagtaa cctttaagaa ccgaataaat aaatggcaaa
     2461 tttcgttcat ttaatttaac tgcactgtta atttgattag ctccatctcc taaagtatgc
     2521 actcctacta cgcggtgact agcatcataa actgcagatc cactagaccc ctctaaagta
     2581 tcaacttggt aatccaagaa catgccatca tcacttaaaa cttgtttttt atctgtatac
     2641 atttgatgaa ttttaaaatt atatgataga tagcctgtga tagtcacagt tattcctgtc
     2701 aaattttttt gactagtagg aagacccaaa gtccctaatt ttgcaccaat gggctcttct
     2761 agaattaata aagccaagtc atattccctt gcattcttag aatttaaatt tctaaattcc
     2821 ttcaaataac gaacttcctt tactttgatc tttccaaata gttcttgact tggactaaca
     2881 gccggaagaa cataaatatc atccgcttct ttcccatagt catgtctgta gtagttgtga
     2941 gccgctgtaa ttaacacatt atctttaata aaattcgcac ttcccctccc ttctacattc
     3001 attccatcgg ctactccata atacttagat aaaaccatag ctgaagtcga atacggatac
     3061 tgtaaagtat tatcaatttt aatcaaatca tcattaccga taatacttct gattgctttt
     3121 ggtagtatga accatacgtt ggtgaaatct cagataatga agaatcatta gactctggac
     3181 ctttttctag tgtctcactt acctcatatt cttcacctgt actagaaata acactcaaag
     3241 cagatactgt cgataactgg ctagccaata aagtactcgc aataattgaa atacccaatt
     3301 ttttataaac agttttcttc attattgtat cctcctaatg taattatagc gtactattct
     3361 aaatttctta atctactata gaatcaagaa atctaccacc ttctttaaat accctccatt
     3421 atcacataaa caggtaaact tttcaattaa tgactgcgct tttcaatcac gctagaggta
     3481 cttacttgct tctttgatac taagttcagc cattctttcc ttgtttttct caataaagca
     3541 tgttacccaa gtgggattcg ttttggagta gtctcgcaga gtccagccaa tggctttatt
     3601 gataaaaaat tctgtttggt tcaagttatg aagtagaatc ttttccatta attgagtatt
     3661 ggtcttctct tttattaaca actggtggtc aatagcgaca cgtctcagcc agatattatc
     3721 tgataggctc cattttatac tcaatgaaaa tcaaagagca aactaggaag ctagctgcag
     3781 gttgctcaaa acactgtttt gaggttgcag atagagctga cgtggtttga agagattttc
     3841 gaagagtatt aagattattt cttctagttc agggtgttca tacaccaaac tccctactac
     3901 tcgatctagg atatctaccg tgtcccacca ggattttgtc acgactaact gctctagctt
     3961 aggcaaatcg gtttccttta gataagactg cattgctttc aaatagttag cagccacata
     4021 ttggtatttt ctaggctcct tttcccagca agtgtctgca aaatcccaat cgataatctt
     4081 tgtttttttc gcttctggta aatattttta tagagtttat ttctttcagg caccgcaata
     4141 cctagaaaag aaaattgatg gcgcatatag gcttccatgg gccttgcttt ttagagtctt
     4201 ttgctgcttc tagctcctaa agtaaatctg ctaaactcat ctaaaactcc tcatgcccca
     4261 ccaaatggtg ctgaaaggcg tagacagccg cctgggtacg atcgctgact tcaagtttgg
     4321 caagaatgtt ggacacgtgg gtcttgaccg tcttgagaga gataaagagg tcatctgcga
     4381 tgcgctgatt ttcgtagccc ttggcgatga gttggagaac atctcgctca cgcgcagtca
     4441 attcttcatg aagttccata tgattgcggt ggtattcaac cttcttgcta acctcttgct
     4501 caatggccag ctcgccagca gctaccttac tgacggcatg aagcaattca tctgcactag
     4561 aagtcttgag catatagcct ttggcaccag catctaagac tggcatgatt ttttcattgt
     4621 ccaaataaga ggtcacaatc aaaatcttgg cttcaggcca ttctttaagg attgctaagg
     4681 tcgcgtcaat tccattcatc tcaggcatga caatatccat gacaatgaca tctggacgca
     4741 gttccaaggc caagtcaatc ccttgagacc cgttggacgc ctcacccaca acttctacat
     4801 cgtcttggag gtcaaagtag cttttcaaac ccaaacggac catttcatgg tcatctacta
     4861 gtaaaatttt catctttact cctttatcat tccttatcta acaggggaat acggatatca
     4921 actgccagcc cttgcttggg agctgttaat aactgaaccg tccctgccat atcttcaacc
     4981 cgctccttga tatttcgcag tccataactc aagtcgtcta agctccctaa ctggaaacca
     5041 atcccattgt ccaccacctt cagttgcaat tcaacatctg tctgatagag gtagacatct
     5101 aggcaagatg cctgggcatg gcggagcgta ttgctaatca actcttgcag gatacggaag
     5161 atatgctcct cgattttctt aggcaatttc gtcatattct gcttgagact aaccctaaga
     5221 tcactcttgt cctcaagctc ttttaagaga atttgaatcc cttctatcaa gctcttctgc
     5281 tccagttcaa ctggtcgcaa atgcaagagc aaaacccgca aatccttctg ggctgtttct
     5341 aaaatagctg tgacactctg caactgggtc tgcatctttt ctctatccaa tttcaaagcc
     5401 tgctgactga tacccgataa aatcatgtgg gccgcaaaca actcctgact gactgtatcg
     5461 tgcaaatccc gagcaattcg cttccgttcc ttctcgatga tttcctcttc ctgagcaagg
     5521 ctatgatttt cagctttttg aagagcctct gtcaaaaggt taagtttacc tgataaggac
     5581 ttgaaactgg catccaaatc ttgatctaca acctgaacta cttcttgccc tgccaataaa
     5641 cgcttgagat tagcctgtat ttttcttaga gaaagctctt cgatccctcg ccaaaacagg
     5701 gctaagagac aggtcatgga catgctgaaa accaacaata aaaagacaaa tttttctgtt
     5761 ttttcgacat cgtgcaaaaa gatagaccag tcaaaatcaa gtatttccag caagctgtgg
     5821 gagaaaaaaa agacaaatag gaaggaggtg agagcaataa tgacataggc ttgttttttc
     5881 atcctctaac cacctccaca tcaccaatca tagtggtcaa gaaaatcttg acactcttgt
     5941 tactcttgag atagtctttt gtttcttgat gatagtgttc attgcggagg gctcgcttgg
     6001 gctggttgaa aaaagtcaaa tccccataga gacagttaac gctgagactg acttccacat
     6061 ctacaggtac gatgattttg gtcgttccta ccatctttct gaggataatg acattgtcat
     6121 gattggttaa gatgaccctc tccagatgaa tagtgtcctt gcccatgaag cgaaagagat
     6181 tgatatcatc gaattggcaa gtctggtagc ttgaaaaatg atgaagattt ccaaaccaac
     6241 gatttttctc cttcttaacc gtcacaacct cttcaaaaac caaattggtc tgctcttttt
     6301 cctggttcat catcgggtaa agaagaaaga ggctatagat aaccgcaaca aaaatagcta
     6361 gaatcacaaa aggattgagc ataacgatga aaaagaagag aatggttacc gctactaaaa
     6421 gaagattatt tccctcttta ccagtgtagt agcgaatcaa aagcaaaaag aggaatagta
     6481 tcagcagaaa acgcgaaaaa tgctctgata ccatcaaaat cagagctcct gtcagaagac
     6541 aggcttcgat aaataaaaag attttaaatt ttctcatagg ttcatcctct cccttctatt
     6601 ttatcacaat tcaaaaaagt cacctcagtc tgaggatgga aaaaaaggcg ctggttacgc
     6661 ctttttcatc tgatcatttg cttcttttaa ttttccataa agaagatagt ctactttttg
     6721 tagatctgct atggtggcac agttaaggga acacataatc aagcgtagat ctgctttcca
     6781 gccttggaca atgccaatca cttcttcaac tgtgtaggtt tcaaccaatt ccagaacggt
     6841 tcgtgacaat cccacagcct tagcaccaaa aaccaagcac ttaatcatat ccagcggatt
     6901 ccgaacccct ccactaacca agagttcgac cttatctttc cattcttggg cattgagaag
     6961 ggcctgcatg gtagactgac cccattgatt gaggtaatca cgctggctac tacgacggtt
     7021 ttcgatatag gcaaagctgg tgccaccacg acccgatagg tccactgtac gaacaccgaa
     7081 ttcataggct ctttcgattg tcttggcatc cattccaaag cccacttcct tgaggacaat
     7141 aggaacggga atttgcttgc tataatctgc tagatgcgat tgccagcttc taaacttcct
     7201 ttctccctcg ggcatgagta attcctgcat gacattgaca tgcacttgca atagcacagg
     7261 attcatctct tctacagtct gaagtcctaa ctcgacaggc ttgtccaatc caatattggt
     7321 tccaaggagg agattgggat gactagactt gacagaaaaa gaatcatccg ttggattttt
     7381 gagggctgcg ctataagaac ccgttacaaa taaaatacca caggattccg ccacctgagc
     7441 cagcttttga ttgatttctc ttcccttatt acttccacca gtcatggcat tgatataaaa
     7501 aggaaagtcc cactttcgac cagcaaactc tgtcgaaaga tcgatttcat ccagattgta
     7561 aagaggcaag gaagaatgaa tcagctccac ctcatcaaag ctattatagg aacttttctg
     7621 ctcaagggca tagagaatat gctcgtcctt acgatttgtc gtcatgtcct atcctttctt
     7681 gatataagag ctcaatcccc agatcggccc aacgattttt taaggttttg gttgattgcg
     7741 catcaaaact cagggcgatg ccacagtcac caccaccagc accactactc ttggcaacgg
     7801 cctgcaaatc ttgactggct tctttcaact gtctaagcaa aggcgtgtaa atatctgtac
     7861 tcaagccttc taaaagcttg ctggctactt ctacttgctc gataactttt tcggctttcc
     7921 cctgctccaa ggcttcgacc aaagaaacca ccgtttcttt tgaggaactt aaaaaatttt
     7981 gattgatatt ttgcttgatt tgctggacca tgtgactcga tacagccact tccttggtcc
     8041 atcccactaa gaaatcacat tctaaagttg gtttcacttg tgagataaaa aatccccaat
     8101 cacgctccag aactgtcgcc aagttttctt cttctaacca agcagccgcc ttctggcgat
     8161 caaatgactg gtagagaacc aaatcctctg ccacaataca ggcaaggtcg cccatggaac
     8221 cattgtctcc tcgcttgagc aagacagcgc tagtcagctt gaacaagaga ttctgatcaa
     8281 ccgaaagatt atagagagcc aataaagcct tgacaaccaa gacaacgacg ctgccactag
     8341 aacctagacc aaactttttc ccttctcgtt ccattttgcc acagattttt agggaaaaag
     8401 gtcttaaatt ctgaccgcga acagcgagga agtctcccat caaagcaatc gtttcttgaa
     8461 tcaagctgta gtcaggatta ggccttaagt ccactgcgaa atcaaacata tctgaataga
     8521 tacggtagct gtcagaaaaa gcaatctcag ccctcatata gatgggaata tcctttatca
     8581 aagctaactg ccctggctct aaaatagcat attcacctgc ccaatagagt tttccgcaag
     8641 ttttaacagc aatcatcttg actcaaatcc tttgtttttg acacaatcaa gcgataacga
     8701 tgaccgaaaa tttctgataa atgctccaag tctttctcct gacagaagac cttaacattg
     8761 ggaccagcat ccatggtaaa gtagcaggcc tctcctttct cacgaagctg gcgaacaaag
     8821 tccatagcct cataagaggc atccgtcaga taagaaaagg ctggactagc agtctttgtc
     8881 gtagcatgca tagccagggc atttttctcc gttaattctc caatcttggc aaaatcattt
     8941 tccttgagat aaatcagcat atcctgatag tccttctcag actgacgaac ccagtcgtca
     9001 aaagtcgtcg aggtttccac acaaagtttc atcccgtcac ggctagagat tggttttttc
     9061 ttgtcctcta gcaccaacat aatcatagct agtttcaagt ctgtctctac agggtaaatt
     9121 tctccactat ccttatccca ggctcctagt ggtccataaa aactccgaga agaagaacct
     9181 gaggcaaatt tggcttcctg tgccaactga cttctatcca atccaagctt gaaataagca
     9241 ttacaagcct tgaccagggc ggacaaacca ctagaacttg aggacagacc cgctgccgta
     9301 ggcatattgt tttgagtatc gatacggaca aagccctcac cagctggacg ataacggtca
     9361 ataatcttac tcatcttggc atgctcgacc tcattttgta gctgaccatt gatgtaaaat
     9421 tcgtcagctg ttacattggc tggtaaaggc gacaaggtcg tctctgtata catattttcc
     9481 aaagttagag aaatactgct agtagcaggc accatctctt tttctttttt ctttccccaa
     9541 tatttgataa tagcaatatt tgcgtaggaa cgtactgtta caggctctct atccatgtct
     9601 gaacagctcc tttctcttct aatctttctg ctagttcttg tgcgtgtgtc aaattggtta
     9661 ccaaggctat gatacaacct cctagcccac caccgctcat cttggcaccc agagcaccat
     9721 ggctaagagt cgtttcaacc aaaaagtctg cctcagggct actgactcca atttctttta
     9781 aatgtaaatg cgcttgactg aggatttgtc ccagtccttc agcatctttt tgtgaaatcg
     9841 caacttctgc ttgctgggtt aattctccca aggcatgcaa aaacggtagg gcatccttgc
     9901 ccttattttg aaccacttgg atggcttcac gagtatgacc ataaacaccc gtatcggcaa
     9961 tcaccaaata ggcggataaa tccatctcaa gttctgtaaa tcctacgttc ttgataaagc
    10021 gaataggttg gtcactaaga caggtcttag catccaaacc actaggattc atatgggcaa
    10081 tcatttcagc tcgattgacc aagatttcta gtacatcatg aggcagatca gcctgatagt
    10141 agtcaaatac tgcacgaatg gccgctatgc tgatagccgc tgacgaaccc atcccccgtt
    10201 tctcagggat agccgagtca atctcacaac gaatgcaggc ttctgtgata ttcaaatact
    10261 ccagtgaggc ataaaccgcc atggacaagg tatcctcctc ataaaggcgc caaggactct
    10321 ctgcaggaac taccttacag gtcacctcca cctccaaaag aggcagggaa atggcaggat
    10381 aaccgtaaac gaccgcatgt tcccctatta aaattatctt actatgtgcc tgaccgacac
    10441 caactttttt tgtcattttt tccttttact agacgaaaaa acgtcttatt tttcatacaa
    10501 gtattaattc tttcctatct attttattat attttcacaa aaaaagcgat tgtttccatt
    10561 cacaatcgct tctttcatta ttgaacccat tcgccattat agttgacaga atagccatct
    10621 acggtcgtat tcactgccaa ggcacctgag ctataagcgt agtaccatct gccattgacc
    10681 tggaaccaac ctgtcgtcat agaacgacga aagaaactcc ataccattaa gtaaagagga
    10741 aagtcgtgag ggagcatgcg ccattgacaa cctgttttag tgacgtacaa agtctcatta
    10801 acaagtactc gtttcggcca tttataggtg cggtgtttgg agaaataggg ttcaatctta
    10861 gcccattctt gatcgtttaa atcagtatca tatactttgc gtgtcataac tctagtttaa
    10921 cattttttgt gaatacaggt tctaaataat cgaccacgaa aatttcttaa gtggaaaacg
    10981 ccttatgaag tatgctacgg gaaagttatg cacttaattt gacaattcaa gatgtaaaaa
    11041 tatatactat agtagattga aactagaata gtacacctct acttctaaaa tattgttaga
    11101 aatcgatttg actgtc
//
LOCUS       DAVKGC010000061        10975 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKGC010000061 DAVKGC010000000
VERSION     DAVKGC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.061"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            11..733
                     /locus_tag="VW049_001362"
     CDS             11..733
                     /locus_tag="VW049_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9432462.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VW049_001363"
     CDS             730..1578
                     /locus_tag="VW049_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9432463.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VW049_001364"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VW049_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9432464.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2662..3153
                     /gene="tpx"
                     /locus_tag="VW049_001365"
     CDS             2662..3153
                     /gene="tpx"
                     /locus_tag="VW049_001365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9432465.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEDLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3249..5957)
                     /locus_tag="VW049_001366"
     CDS             complement(3249..5957)
                     /locus_tag="VW049_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9432466.1"
                     /translation="MIKRKTYWKDLVQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAS
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5959..6660)
                     /locus_tag="VW049_001367"
     CDS             complement(5959..6660)
                     /locus_tag="VW049_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432467.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANCDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6910..9321)
                     /locus_tag="VW049_001368"
     CDS             complement(6910..9321)
                     /locus_tag="VW049_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9432468.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASNGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAALKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLQNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9374..10066)
                     /locus_tag="VW049_001369"
     CDS             complement(9374..10066)
                     /locus_tag="VW049_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9432469.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10601..10957)
                     /locus_tag="VW049_001370"
     CDS             complement(10601..10957)
                     /locus_tag="VW049_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432470.1"
                     /translation="MSGIVGLVGGILLLIGPFVLLGTAVNTAATTLNGGATAGAFSGV
                     ALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGWVGGILAII
                     GGSLFLATLKKFKSEE"
BASE COUNT         3202 a         2517 c         2058 g         3198 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tacaaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattacc ggcatcattg gtccaaacgg
      121 cgctgggaaa tcaacactat taaaaggtat gctgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa ggctaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgactctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ctgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagcc
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat atcctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaatct ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gtatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattggt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtt ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 tgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctctgaaaa acgtcattct catgtgagct ggcgtttttt
     2581 ctatgcccac atttccggtc aaatcattgg aaaattctga ctgtttcaga tacaatggaa
     2641 gaaaaaagat tggagtatcc tatggtaact tttctcggaa atcctgtgag ctttacaggt
     2701 aaacaactac aagtcggcga caaggcgctt gatttttctc ttactacaac agatctttct
     2761 aaaaaatctc tggctgattt tgatggcaag aaaaaagtct tgagtgtcgt tccttctatc
     2821 gatacaggca tctgctcaac tcaaacacgt cgttttaatg aagaattggc tggactggac
     2881 aacacggtcg tattgactgt ttcaatggac ctaccttttg ctcaaaaacg ttggtgcggt
     2941 gctgaagacc ttgacaatgc cattatgctt tcagactact ttgaccattc tttcgggcgc
     3001 gattatgccc tcttgatcaa cgaatggcac ctattagcac gcgcagtctt tgtcctcgat
     3061 actgacaata cgattcgcta cgttgaatac gtggataata tcaattctga gccaaacttc
     3121 gaagccgcaa ttgcagctgc taaagcccta tagaaaaaat cctctgtcac aaagagttcc
     3181 ctgctctttg aaacggagga tttttgaata ttagtaaata gaagttcaat cagctaaaaa
     3241 taactacctt actctacaga tttcagggct tctaacatat caacctttct cagataataa
     3301 ttgacgaaga aaccaagcaa ggtcaaaatg atgcttactg ctgccactgg gattacatag
     3361 acttcccagc ctacctgcgg ataaaagaga atagtcgcag gcgaaatcat ttgaatcaaa
     3421 aattggtgta aatagaaacc agctaccaga ccaagtacga ttcccacaag ggatagcaca
     3481 atcgtctcac ggtaaatgta gagtgtgact tcattattat gaaaaccgag aaccttggta
     3541 gtggagagtt cacggattct ctcagctacg ttgatattgg tcagattgta aaggatgaca
     3601 atagctaata gaaccgatac gatgaccaag atggtcatgg tctgattgag tgagctagcg
     3661 atagagtcga agagtcgaat ggctgaagca ttttggacaa cgctggacac cgcagattga
     3721 ttcataagca agcccgcctg actttcgata ctagttgcac tggtatccct taatgagacc
     3781 agataagtgt tggcttgggg tagctgtccg taaagttgct catagctagc ctgactcata
     3841 taaataaagt gaccaacgta gttctcagta atagcagcga cctttagttc cttaccttca
     3901 atttctaaag tctgcccaac cttgacacct gccagctggg cgagtttagc tgtaataacg
     3961 atgccatctt ttaatgtcag ctcctgctga tgatgttgaa gatggataaa gggagtcaaa
     4021 tcttccttct ctatcatcat aagagtaatg ttttgaagac cagccttgcc tttgaaatcc
     4081 ttgtctagcg ttttagaata gattttctgg taggctagta tctcctgccc tttcaatact
     4141 tctgctagct ctaccttgtc ctgattggtc gcactaggat tttcagagac aagcatctga
     4201 tactgttgga tttgttgaaa ctgtttagac ggaactcctg ctacagaaga ttggattccc
     4261 aaacctgcaa agagcagagc tacagaacct gccacaccaa agattgtcat caacattctc
     4321 tgcttataac gaaagatgtt gcgggctgtt accttatgag taaaactgag acgacgccag
     4381 ataaaaccga tacgctccaa taagatttta gctcctttga caggaggttt aggaagtaga
     4441 agctgggctg cttcggcatg aagttccctc caagccacca gataggctgg taacacactc
     4501 gccaacaagc tcaagacaag agctagtaag ctataggtcc aatagaactg aatctgggtt
     4561 tctcccacca ccatgccttt tgtaatgaca cttgaaatta cactggctag caaataatga
     4621 ccaagtatac tacctagagc cgttccgaca gtcccagcta ctagtccata aaggagaaac
     4681 ttggtgataa tatccttact acggtaaccc aaggccttaa aaatccctgc atgagttcgc
     4741 tcttcgtcta caaagcgagt catggtcgta aaggtcacca tggatgctac ggcataaagt
     4801 accacaggaa agatattgcc cactgctcga atactggatg aagcattgct atacataaga
     4861 tagccctgac cacctggcat ggtctgacga ttatagacct gataccttgg ctccgccaaa
     4921 tcgtccaaga cttgctgatg tttttctaat ttttcctttt cttgggctag attttgttca
     4981 gcttgcttta gtttatcctc ttccttgccc aattcctgct tggcttgggt aagttgagca
     5041 ctagcctgct ctctctgaac ttgaggaaac aaggctagtt gactttcttg agcctgtata
     5101 cgagcttgag cagctgctaa acgacgcttg ccttcctgca aattagtctg agccttgtca
     5161 agggtctctt gccccttgtc tagagactct tgtccttctt ttttcaaaag ttgcagacgt
     5221 accttgccat tatctgataa agtttgttca aggtcttcct gatgttgctt ggatttttct
     5281 tcataagctg atgaaaaggc atttaaacct gctaaatctt gatatttcaa acgagctata
     5341 ttgtagactt tctgatcaaa ctgactaggt aaaatcaccc cataggctgt cagagtccca
     5401 cttccacttc ctgcgtagcc catatctcgc tgggagagga tttcagccga atccacaaaa
     5461 ccagtaatgg tataagtatg gtcttttaaa gaggaatgac cctcttcttt ttctttaaaa
     5521 ctaatctcct gtcccacgct gtattggcct tgcaaatgag tggccaaagc gatttccttg
     5581 tctgactgag gaagtcgtcc ctttcttagc tgaaaggttg aaattcgctc tggtttggag
     5641 tacagccgaa tggcatcctg cccattatcc atagtcacat ctgtcaaata gccaaactcg
     5701 acctctgcgc cctccgtctg ttttagttct tcttggtctg cttgatccaa gccatagtta
     5761 gacatgactg ccaaatccaa ggtttgagca gttgttaaat aagcattagc tgtcgcctcc
     5821 atgttgggac tggttacttt gaggcctact aaggctagag atcccaacat catcaggatc
     5881 aagatggata aaaaacgccc cttggagcct gtgaaggact gaactaagtc cttccaataa
     5941 gtttttcgct tgatcatgct agtactccaa actgtcaata tcctgaggat gctggttgag
     6001 caccacatcc ttgacactgg catcgtgcat ttgaatcacg cgatcagcaa tgggcgccaa
     6061 agctccatta tgagtcacga tgatcaccgt cgctcccttt tgacgagaca tgtcttggag
     6121 aattttcaaa acctgcttgc ccgtctgata atccaaggct ccagtcggtt catcacaaag
     6181 gagaatttta ggatttttgg ctaccgcgcg tgcaatggag actcgctgtt gctcccctcc
     6241 agaaagctgg gctggaaagt tattgagacg atgagccaga cctacatctg tcaagacttg
     6301 atcaggattc aaggcatctg tcacaatttc agaagccagt tccacatttt ccttagctgt
     6361 cagattagaa actagattat aaaactgaaa aacaaacccc acatcatttc tacggtaatt
     6421 ggtgcgctgg tgggaactat aatccgcaat attaacacca tcaatccaga tttccccttc
     6481 atcattggta tccattcccc caagaaggtt aagaactgtt gacttgcctg cacctgaagc
     6541 accaaggata ataaccagct cccccttttc aatctcaaaa ttcacatcac aattggccac
     6601 aatctccgtg tccccaacct gataacgctt gtaacagtgt ttcatctcaa tataagccat
     6661 cagacccacc ctctatcaaa caaattatac tcctcgaaaa tctctcttca aaccacgtca
     6721 gcttccatct gcaacctcaa aacactgttt tgagcaacct gcggctagct tcctagtttg
     6781 tgctctttga ttttcattga gtattacttc ctattatcat tataacgttt tttcaactag
     6841 ttgaaaactg cttatattct taaatcctta ttaaaaggca gtttcaaatt actgccttat
     6901 aacatcttct taaaaataaa attgaacaag atcaccttct gctgttgcca ccgaaataca
     6961 atctttcccc atgcatttcg cttttatttt ttcttgattc attttaatca cactcttctc
     7021 aatatctgga taagaaactc gcaaatctcc tccactcctt gataaaatgg tcaactgtaa
     7081 gagtttttta tcttcccagc tcatgctcac ttcaaaatgt ccacgtgcca ttaagcctga
     7141 aacagaacct gttgaccaag catcaggtag ggcagctaga ggtaccagat aagctgcatg
     7201 agactggagt aacatttctg ccatgccact agtagcacca aaattaccat ctatctgaaa
     7261 aggaggatgg ctacaccaaa gattttgcaa ggtggatgtc tttaactgct ctgccaataa
     7321 tttatgggct cgattgccat ctcccaaacg cgcccagaga ttgatcttat tagccttgga
     7381 ccagcctgtg ccgccatctc cacgatcatt gaggctagca cgcgccgctt caatatactc
     7441 ttgtcccttg tagctaaaga gattgccagg atagagtccc actagatggg aagcgtgccg
     7501 atgctgggcc tccactttct cattttgaaa atactgctct tcctcctcat accactccct
     7561 gattcgacca gattgagtga tttgcaaagg atttagtaaa tcagacttct ccttaacctc
     7621 agtcaacaag tcctcatcca gtcccaattc ctgagcagcc tgaataaaat catgaaataa
     7681 ctgccaaatc agagattggt cataggtatt gccaatcgaa atcggcccat gttctgggga
     7741 ataagacgga gaagacaccc aacgctgcgc ctgctgatcc ttatgtaaaa aggcattcca
     7801 aaaacgaacc gtttccctca acatgggata aattttctcc ctaagatagt cttggtccct
     7861 ataaaatgaa taggcttcat aaacggtttg catcatccac gcattggcag ctggtgacca
     7921 accccaatag taatcccaac caggtgccgt ccaaccaaag ggagtcgctt gagtatgaac
     7981 caaccaacca ttttcctcac ctttctgaga gacgattcct gcatacttta aagccgctag
     8041 acgaccatag acacgcaaat catctacata gttgatgact ggaaagaccg tctctagaag
     8101 attggtaaca taggctggcc aataattcat ctgcagattg acatttaagt gatagtccga
     8161 attccaagga ggattgtcga ccgcattcca gactccctgt aggttagctg gtagagcatc
     8221 tgggcagtct ctggacgaac taatcaataa ataccgtcca tactggaaga acagctcctc
     8281 caaagcctgc ccttcttgtg gcttataatt ttttaacaaa tcatctgtag tggatgcgtc
     8341 aacatcagct tccaaatcca attgaacacg ctggaataag gcttggtagt cctcgatatg
     8401 ccttgatttc aattgggtat agcccttttc tttagctgtg tccaccaagt ctatcacctg
     8461 ttgctctaaa tctagtttct tgcgataatt gctagcagga ttttgggcaa aatccgtctt
     8521 agcggccaag aagagattgg cataactggc tcctgatatc tgaaccctat ctgaccaaac
     8581 tctaatatct ccatccgttt cccaagctag ataactagca aaccgcagat cattatcctt
     8641 aactcttccc ttcatcaaga tatgagaatc agtaatatcc aacttacact ccttgtaatc
     8701 agatttttcc tgctcatact ttccattaga agccaaatca caggtcaagg atagttctat
     8761 agtaaaatct agagtttcca acccttcctt agtaaaacat tgaaccaaga gatcatctgg
     8821 aaaactcgca aaagcttcac gttcaaatcg cgttccctta tagacataag aagtcgtcgc
     8881 aagtgcctta ctaatattca actgtctctg atagtccgtc acctgagaca aagtcgtacc
     8941 ttgctggctg aactcaatgt gaatatcccc aaaagacaga taggtcccat attgactcgt
     9001 ttttggccca attaggtgct gctcagccag ttccttagcc agattgtaat ctctcttctc
     9061 caaagcctgc cgaatctcag ctaaaaaaac atactgatcc tgaagatttc caccctgata
     9121 atctgaacta tcaggaagtg gacctccaga ccagagactt ttttcattaa attgaatccg
     9181 ttcagcccct ataaggccaa atacttttgc tcctaaagaa ccattaccta tcggtaaagc
     9241 ttcttcttcc caacccatgt aagtggttga agctggttgc ttgtaggcca gtacataatc
     9301 ttgtttttta ttccttatca tacttccata ctaacataaa aagcctagaa atcaatccta
     9361 ggcttacaat tttttatttt ccaaggtagt attcagaaac aacgttcaat ttttcgtcga
     9421 attcgaatac caatggtggg aagttaggga tttccacgtc catgatctca tcatctgaca
     9481 aacctttgat gtgttttaca agggcacgga ttgagttacc gtgagctcct acgaatacgt
     9541 ttttaccatc tttaagagct ggagcgattt tatcttccca gaatggaaga gcacgttcca
     9601 aagtcacttt caagttttca gcatctggga taactgagtc gtcaagtgaa gcgtaacgac
     9661 ggtctgtgtg agctgagtgc tcatcatcac ggtccatgtt tggaggcaat acatcgtatg
     9721 aacgacgcca gatgtgaact tgctcatcac caaattgttc agcagcttca gctttgtttt
     9781 taccagtcaa accaccgtag tgacgttcgt tcaagcgcca tgatttttca actggaaccc
     9841 acaattggtc agaagcttca agagccaagt tagttgtttt gatagcacgt ttcaatactg
     9901 aagtgtaagc ttggtcaaat tcgataccag cttctttgat caatttacca gcgtcaatcg
     9961 cttgttgtgt acctttttca gacaaatcaa catcagccca accagtgaaa aggttagctt
    10021 tgttccattc agactcaccg tggcgagcaa aaaccaattt taccattaga tggattctcc
    10081 tttaaatttt ccgaggtttc ccctcgttta tctattctat tttacacaat ttttcacaaa
    10141 aaagctagtc aaaaccaaaa aggaaaggac tgtttgacaa tgtttggcaa tcacattgtt
    10201 tttggttgtt tctattctat cttcattatt taaccttttt ttctgcgcat cgaataattt
    10261 ttgagttgac tcttttagtt tagtaaacga gtcattattt tccatagctg tgcctttgaa
    10321 accttcctca gctttagtaa aataatcatc aaaaatattt ttataaagat ctatataatc
    10381 tgcataagtg cgtattgatt caatcgcatt gtctgttaca accggtgtat cgaaagtgat
    10441 tgtttctttt gaactattgg atgttgctac tgtcttctca tcattacttg ttgaagtatt
    10501 ctttgaactt tatgttccac atgcaactag aaaagaaaga gataatagag ctactcctat
    10561 accgataaac ttttttgttc ttttcatgct aaaatacctt ttattcttct gatttgaatt
    10621 tcttcaatgt tgcaaggaat agagatcctc cgataatagc aagaatcccc ccaacccatc
    10681 ctaagaatgg aatgagacta actccaccag aaacaatcat tagtacagac ggagctgcac
    10741 ctacacgctt atctccttta tagtaaacaa tagcaatgat accaagaaca agatttgcaa
    10801 tcttcaaggc attcaagagt aaggcaacac ctgaaaaagc ccctgcagta gctcctccat
    10861 taagagttgt agcagctgtg tttaccgctg ttcccaacaa gacaaaagga ccaataagaa
    10921 gtaaaattcc tcccacaaga ccgacgatac cactaattaa agcaagtgtt tttaa
//
LOCUS       DAVKGC010000062        10924 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKGC010000062 DAVKGC010000000
VERSION     DAVKGC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.062"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            347..1510
                     /locus_tag="VW049_001371"
     CDS             347..1510
                     /locus_tag="VW049_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9432471.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASELENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSTYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVVAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1507..2184
                     /locus_tag="VW049_001372"
     CDS             1507..2184
                     /locus_tag="VW049_001372"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9432472.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLGFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAAMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTMSMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2174..3028
                     /locus_tag="VW049_001373"
     CDS             2174..3028
                     /locus_tag="VW049_001373"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9432473.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGTYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3047..4114
                     /locus_tag="VW049_001374"
     CDS             3047..4114
                     /locus_tag="VW049_001374"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9432474.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELENGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4124..5290
                     /gene="aroC"
                     /locus_tag="VW049_001375"
     CDS             4124..5290
                     /gene="aroC"
                     /locus_tag="VW049_001375"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9432475.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5300..6403
                     /locus_tag="VW049_001376"
     CDS             5300..6403
                     /locus_tag="VW049_001376"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9432476.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6414..6752
                     /locus_tag="VW049_001377"
     CDS             6414..6752
                     /locus_tag="VW049_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9432477.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6844..8127
                     /gene="aroA"
                     /locus_tag="VW049_001378"
     CDS             6844..8127
                     /gene="aroA"
                     /locus_tag="VW049_001378"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9432478.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRATGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8120..8596
                     /locus_tag="VW049_001379"
     CDS             8120..8596
                     /locus_tag="VW049_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9432479.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGESAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIVADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8593..9441
                     /gene="pheA"
                     /locus_tag="VW049_001380"
     CDS             8593..9441
                     /gene="pheA"
                     /locus_tag="VW049_001380"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9432480.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9438..10712
                     /gene="psr"
                     /locus_tag="VW049_001381"
     CDS             9438..10712
                     /gene="psr"
                     /locus_tag="VW049_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9432481.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSSPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQSFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLTQMGLR"
BASE COUNT         3171 a         2075 c         2620 g         3058 t
ORIGIN      
        1 ttttctataa aatcaaattt tccagatatc tactgaagca gatttatttc ttcatctttc
       61 ttctactacc tttgtcacta aatttgacag acacatattg ctgattcaac ctctctacct
      121 ataatctcac agttaacctg aggctagttt catagtttgc tatttgattt tcgttgataa
      181 aaagaaatgc tcctccaaga cggaagagca ttttttattg tgattcttac acatttactt
      241 cctagttttg caggaagtct agtataccat tttttaaaag aagagtaaaa aagcaagggt
      301 agtttctttt ttgagaattg tctaaaagtt tgatataatg gtagttatga atagaataag
      361 agtcagcaaa agggttgaaa agaagcttgc taaggggcta gttttactag aagccagtga
      421 acttgagaat gtcaatctta aggatcagga agtagaggtg cagggtcagg aaggaaactt
      481 tcttgggact gcctatcttt ctcagcaaaa caagggcttg ggctggtttg tcagcaaaga
      541 caaggtggcc ttcaatcaag ctttctttga aaccttgttt agaaaagcca aagaaaagag
      601 aagcacctac tatcaagatg atttgacaac tgcctttcgt ctctttaatc aagagggaga
      661 tggctttggg ggtctgacag tggaccttta tggcgactac gccgtttttt cttggtataa
      721 ctcttatgtt tatcagattc gtcagactat atcagaagcc tttagacagg ttttccctga
      781 ggttttaggg gcttatgaga aaatccgctt taaggggttg gactatgaat ctgcccatgt
      841 ttatggtcaa gaagcacctg actttttcac tgttttagaa aatggtgtcc tgtatcaagt
      901 ctttatgaat gatggcttga tgacaggaat tttcctagac cagcatgagg ttcgcggtag
      961 tttagttgac ggcttggcta tgggtaaatc cttgctaaat atgttttcct acacagcggc
     1021 tttttcagta gttgcggcca tgggaggagc tagccagaca acttctgttg atctagccaa
     1081 acgttcacga gaattgtctc aagcgcattt tcaggcaaat gggctcagca cagacgagca
     1141 tcgttttata gtcatggatg tctttgagta tttcaaatat gccaaacgca aagacttgac
     1201 ctacgatgtg attgtcctag atccgcctag ctttgctcgg aataaaaaac aaactttctc
     1261 tgtggccaag gattatcaca agttgatttc ccagagtctt gagattttaa atccgggagg
     1321 gattatcatt gccagtacca atgctgccaa tgtttcccgt cagaaattta cagaacaaat
     1381 tgataaaggc tttgcaggaa gaagttacca gattttaaac aaatatggtc ttccagcaga
     1441 ttttgcctat aataaaaaag atgaaagtag taattacctc aaggtgatta gtatgaaggt
     1501 tagtaaatga aattaatcgt ttcagtaatg ccaagaagtt tagaggaggc tcaggctctg
     1561 gatgccacga ggtacctgga tgccgacatc attgaatggc gtgccgacta tctgcctaaa
     1621 gaagcgattt tgcaggtagc tccagccatt tttgaaaaat tcgcaggccg tgagttggtt
     1681 ttcacgctac gaactcgttc cgaaggtgga gaaatcgacc tttctccaga agagtatatc
     1741 catctaatca aggaagttgc gcaactctat caaccagact atattgattt tgagtactat
     1801 agctacaagg atgtttttga ggaaatgctg ggcttcccaa atcttgtttt gagttaccat
     1861 aatttccaag aaacacctga gaacatgatg gaaatcttgt cagagttgac gatcctaaat
     1921 ccaaaacttg tcaaggttgc ggcgatggct cacacggagc aggatgtctt agacttgatg
     1981 aactatacac gaggctttaa aaccctcaat cctgaacagg aatatgtgac catgtctatg
     2041 ggtaaggtgg gcaaggtctc tcgtatcact gcggatgtga ctggttcgag ttggtctttt
     2101 gccagtctgg atgaggttag tgcccctggg cagatttctc tagctagcat gaaaaaaatc
     2161 agggagattt tggatgaagc ttgatggcta tacacgttta gctgcagttg ttgccaatcc
     2221 tattaagcat tctatttctc ccttcatcca caatagagcc tttgaggcga cagctaccaa
     2281 cggtacttac gtggcttggg agattgaagc gagtgacttg gcagaaacag tggccaatat
     2341 tcgtcgctac cagatgtttg gcatcaatct gtccatgccc tataaggagc aggtgattcc
     2401 ttatttggat gagctgagcg atgaagcgcg cttgattggt gcggttaata cggttgtcaa
     2461 tgagaatggc aatttaattg gatataatac agatggcaag ggatttttta agtgcttgcc
     2521 ttcttttaca atttcaggta aaaagatgac cctgctgggt gcaggtggtg cggctaaatc
     2581 aatcttggca caggctattt tggatggcgt cagtcagatt tcggtctttg ttcgttccgt
     2641 ttctatggaa aaaacaagac cttacctaga caagttacag gagcagacag gctttaaagt
     2701 ggatttgtgt gctttagaat atgtttctga actgcaagca aggattgccg agtcggattt
     2761 gctagttaat gccaccagtg tgggcatgga tggccaatcc tctcctgttc ctgaaaacat
     2821 agtcttacca gaaactctat tagttgcaga tatcatatac caaccctttg aaacaccgtt
     2881 tttgaaatgg gctagaagtc agggcaatcc agcagtcaat ggtctgggaa tgttgctcta
     2941 tcaagctgca gaagcttttc aactgtggac aggcaaggaa atgccgacag aagagatttg
     3001 gcagtcttta acagaaaaat atcaataaag aaagggagac tctcctatga aaatcagaat
     3061 cgatattcct catcatcctt atgatattca gattgaaaaa ggttgtatgg cccaggctgg
     3121 tcagtggttg cgagaactct ggcaaccgca aaaggtagtc attgtgacag ataaccatgt
     3181 agcctctctc tatgcagaga aggtcaagct cagcctagaa gatgctggct ttcaggtagc
     3241 tgtttttgat ttcttagaag gtgaagaaag aaagaattta actactgttc agaaagtcta
     3301 tgaatttcta gtcaagcaag gtctgactcg tagcgatgga atcgtggctc ttggtggtgg
     3361 cgttgttggg gacctggctg gctttgtagc ctctacctat atgcggggta ttcactttgt
     3421 tcagattccg actagtttga cagctcaggt tgattcttct atcggtggaa agacaggcgt
     3481 taacacacca tttgctaaaa atatggtggg gacctttgcc caaccagatg gggttctgat
     3541 tgatccactt gttcttgaaa ccctcggaaa aagagagttg attgaaggga tgggtgaagt
     3601 tatcaagtat ggcttgattg aggatccaga actgtgggct ctcttgacgg ggctgaatgg
     3661 ttctgttgag agcattttgg aacatgcaga gaccttgatt gaacattctt gtcaggtgaa
     3721 gcgcaagatg gtggttgaag atgagttgga gaatggtatt cgtctttacc tcaattttgg
     3781 ccacactatt ggccatgcca tcgaagcgac tgccggttat ggcaaggtca tgcatggaga
     3841 ggctgttgcc atgggaatgg tacagatttc caaggttgct gaggaaaaag gcctcatgcc
     3901 agctggcatt acccaatcta tcacagagat gtgtcagaaa ttcggcttgc ctgttgacta
     3961 tgaaaattgg aaagttgaca agctttatca ggctcttact catgacaaga aagcgcgtgg
     4021 taacaccttg aaattggtct tggtgccaga gcttggttca gcgaccattc acccagtttc
     4081 tctggaagag atgaaagact acttggtaaa ataaggagag tgtatgagat atttaactgc
     4141 aggagaatca cacggccccc gtctaacagc tattattgag ggaattccag ctggacttcc
     4201 attgacagct gaggatatca atgaggatct tagacgccgt cagggtggct acggtcgtgg
     4261 tggtcgtatg aagattgaga gtgaccaggt tgtctttact tcgggtgttc gccacgggaa
     4321 gacgacaggg gcgcctatta ctatggatgt catcaataag gaccaccaga aatggctgga
     4381 catcatgtct gcggaggaca ttgaagaccg ccttaaaagc aagcggaaaa ttactcatcc
     4441 tcgcccaggt catgccgatt tggttggggg gattaagtac cgttttgatg atttgcgaaa
     4501 ttctttggag cgttcatcag ctcgtgaaac caccatgcgg gtggcagttg gtgcagtagc
     4561 caaacgcctc ttggctgagc tggatatgga gattgccaac catgtcgtgg tctttggtgg
     4621 taaggaaata gatgttcctg aaaatctgac agtcgctgaa attaagcaaa gagctgccca
     4681 gtctgaagtt tctattgtca accaagaacg agaacaggaa atcaaggact atattgacca
     4741 aatcaaacgt gatggtgata ccatcggtgg ggttgtggag acagtcgtcg gaggagttcc
     4801 agttggtctt ggttcctatg tccaatggga tagaaaattg gatgcaagat tggctcaagc
     4861 tgttgtctct atcaatgcct ttaaaggggt ggaatttggt cttggctttg aggctggtta
     4921 tcgtaaaggc agccaagtta tggatgaaat tctctggtct aaagaagacg gttatactcg
     4981 ccgtaccaat aatctaggtg gttttgaagg tggtatgact aatgggcaac ccatcgttgt
     5041 tcgtggggtc atgaaaccca ttcctactct ttataaacct cttatgagtg tggatatcga
     5101 aacccacgaa ccttacaagg caaccgtgga gagaagtgat ccgactgctc ttccagctgc
     5161 aggaatggtc atggaagcag ttgtagcaac ggttctggcg caagaaatcc tcgaaaaatt
     5221 ctcatcagat aatcttgagg aactaaaaga agcggtagcc aaacaccgag actatacaaa
     5281 gaactattaa ggagttccta tggcaaaaac aatctatatc gcaggtcttg ggttgattgg
     5341 agcctctatg gcacttggta tcaaacgcga tcatccagat tatgaaattt taggttataa
     5401 tcgtagtcaa gcttcgagag atatcgcctt gaaagaaggc atgattgacc gtgcaacgga
     5461 tgattttgct agttttgctc ctttggcaga tgtcattatc ctcagcttgc caatcaaaca
     5521 aactattgct ttcattaagg agttggccaa tttggatttg cgagaaggcg ttattatttc
     5581 agatgctggt tcgaccaagt caaccattgt ggatgcggcg gagcagtatt tggctggcaa
     5641 gtctgttcgc tttgtcgggg cccatcccat ggctggtagt cacaagacag gggctgcttc
     5701 ggcagatgtc aatctttttg aaaatgccta ttatatcttt acaccttcaa gcctgacaag
     5761 tcaggacacg cttaaggaaa tgaaggatct gctttcaggt cttcatgctc gttttatcga
     5821 gattgatgcc aaggagcatg atcgtgtcac ttctcagatt agccattttc ctcatatttt
     5881 ggcttctagt ctcatggagc agactgcggt ctatgctcaa gagcatgaga tggcaaggcg
     5941 ctttgcggca ggtggttttc gagatatgac ccgaattgcg gaaagcgagc caggaatgtg
     6001 gacctccatt ctcttgtcca atagcgagac cattctggat agaattgagg atttcaagga
     6061 acgtttggaa gtgattggtc aggccattag taagggagat gaagagcaaa tttggaactt
     6121 ttttaaccaa gcgcgtgaac aacgccagac catggaaatc cataagcgtg gtggtgtgga
     6181 tagctcttac gacctctatg ttgacgttcc tgatgaagaa gatgtcatcc tgcggatttt
     6241 ggaactgcta cgtggaactt ccttggttaa catccacatc aacgaggaaa atcgtgagga
     6301 tattcacgga atcctacaaa tttcatttaa aaatgctcaa gacttggaaa gagctgagca
     6361 tctcataaca gaaaataccg actatacagt cgtcatcaaa taaggagaag aacatgtcaa
     6421 atatttacga tagtgcaaac gaactcagtc gcggtctacg cggattacca gaatacaagg
     6481 ctgttaaagc agctaaagat gcgattgcag cagatgctga ggcaagcaaa atctttacag
     6541 aatatcttgc ctttcaagag gaaattcaaa aactagcaca gacaggtcaa atgccagatg
     6601 cgtccttcca agctaagatg gaaggctttg gtaaacagat tcaaggaaat agcctcttgt
     6661 cagaattctt taccaagcaa caacaattgg ccatttacct ttctgacatt gaaaaaattg
     6721 ttttcgaacc agtttcagaa ttgctgaaat aagaaaagaa ctatcctaaa gtcaggaatt
     6781 tttaaggaat ttctggcttt ttatgataaa ataggtaaaa gtattgcaag tatgaggtcc
     6841 agtatgaaac taaaaacaaa tattcgccat ttacatggta gtatccgcgt cccaggtgac
     6901 aagtctatca gccaccgttc cattatcttt ggaagtttgg ctgagggtga gaccaaggtt
     6961 tatgatattc tgcgaggtga agacgttctt tcgaccatgc aggtttttcg tgaccttggt
     7021 gttgaaattg aggataaaga tggggttatt accattcaag gtgtaggcat ggctggctta
     7081 aaagcgccgc aaaatgccct taatatggga aattctggca cctcgattcg cctgatttca
     7141 ggtgtccttg ctggtgcaga tttcgaagta gagatgtttg gagatgatag tctttccaaa
     7201 cgtcctatgg accgtgtgac ccttccactg aaaaaaatgg gcgtcagcat ttcagggcaa
     7261 actgaacgag acttgcctcc ccttcgctta aaagggacga aaaacctaag acctattcat
     7321 tatgagttgc caattgcctc tgcccaagtc aagtcagcct tgatgtttgc agccttacaa
     7381 gctaaggggg agtcagttat tatcgaaaaa gaatacaccc gtaatcatac tgaagatatg
     7441 ttgcaacaat ttggtggtca tttaagtgtg gatggtaaga aaatcacagt ccaagggcca
     7501 caaaaattga caggacagaa ggtggtcgta ccaggagata tttccagtgc agccttttgg
     7561 ttagtcgcag gtttgattgc tccaaattct cgtctagtgc tgcagaatgt ggggatcaac
     7621 gaaactcgca ccggtattat tgatgtcatt cgtgccacgg gtggaaaatt ggaaataact
     7681 gaaatcgatc cagtcgctaa atctgcaacc ttgattgttg agtcttctga cttgaaagga
     7741 acagagattt gtggcgcttt gattccacgt ttgattgatg aattgcctat tattgcccta
     7801 cttgcgaccc aagcccaagg tgtaacagtt attaaagatg ctgaggagct caaggtcaag
     7861 gaaacagacc gtattcaggt tgtggcagac gccttaaata gtatgggagc agatattact
     7921 cctacggcag atgggatgat tatcaaagga aaatcagctc ttcacggtgc tagagtcaat
     7981 acgtttggtg accaccgtat cggcatgatg acagctatcg cagccctatt ggttgcagat
     8041 ggagaggtgg agcttgaccg tgcagaagcc atcaatacca gctatcctag tttctttgat
     8101 gatttggaga gcttgattca tggctaaggt gttattaggg tttatggggg ctggaaaatc
     8161 aactattgca agaggcttgg accctaatta ccttgatatg gatgctctga ttgagaagcg
     8221 cctaggtatg tccattgcga attttttcgc tgaaaaggga gaatcagcct ttcgtcaggt
     8281 agaatcagaa gtcctagctg atttactaca aacagaccaa gtcgtgtcaa ctggaggagg
     8341 agtggttatt tctcagagaa atcgtgactt actcaagact aatacagata acatctacct
     8401 gaaagcagat tttgaaaccc tctacctacg tatcgtagct gataaggaca atcagcgacc
     8461 gctttttcta aataatagca aggaagaact agtagctatt tttcaagaaa gacaggcttg
     8521 gtatgaggaa gtggctagtc gggttttgga tgtgaccaag ctaagcccag aggaaattat
     8581 agaggaacta agatgaaaat tgcttatcta ggtcccaaag gatcattttc acaccacgtt
     8641 gtgcagacag cttttcctca tgaggaattg caggcttttg ccaacattac agatgttatc
     8701 aaggcttatg agcaaggatt agtggactat tctgtggtgc cagttgaaaa ttctattgag
     8761 ggtagtgttc atgaaacctt ggactatctt tttcatcagg ctcacatcca agcagtagca
     8821 gaaatcgttc agcctattca tcagcagttg atggtggttc caggccatac taagattgaa
     8881 aagatttttt cacatccaca ggccttggct caaggaaaga aattcatcga tgaacaatat
     8941 ccagaggctc aaatcgaggt aacagctagt acagcttatg cggcccgttt tatttccgaa
     9001 catccagacc agccctttgc agcagttgca cctagaagtt ctgctgaaga atatggattg
     9061 gaactgattg ccgaggatat tcaggaaatg gaagccaatt tcacacgttt ctgggttcta
     9121 ggagctgaaa agcctagtat tcccttgcaa gcacaaactg aaaagatgag tttggccttg
     9181 acattacctg acaaccttcc aggtgcactt tataaggccc tgtcgacctt tgcttggcga
     9241 ggaattgact tgacaaaaat tgaaagtcgt ccactcaaga cagcactggg tgaatacttt
     9301 ttcattatcg atgtggatta taccgataag gacttggtcc actttgccca aaaagaatta
     9361 gaagcgattg gaatccagta taaaattctg ggtgcctatc ctatttatcc aatatcagac
     9421 catggaaagg agagaagatg agtaaagaaa atcccttaag tcatcatgag cagttgcgtt
     9481 atgattattt gctaaaaaat attcactatc tcaatgagag agaaaaaaat gagtttgcct
     9541 atttgcaaga aaagctaact cttgctaggg gaaatagtag ctctagcttg gaacaagaaa
     9601 gagaagagca ggttgactta ccaagctatg cgaaccggag tcgctcacaa tccaaatcac
     9661 aagcactctc ttcccctcca aaaaagaaaa gacggaagct ccgtcttaaa cgaattttta
     9721 tggtgatttt ttcacttttg gtctgtgtgg ctttggccat ggtattcatg tttttgcgtg
     9781 gttaccaaga tgctagcgca aagaaaactg ctgatgctcg ggcagctcaa gtagaagtct
     9841 ttaatggtca ggacactaga gatggagtta atattttaat catgggtact gatggtcgaa
     9901 tcggccagaa cagtgttgag acacgaactg actctattat ggtattaaat gtcgggggct
     9961 cagataagaa aatgaagcta gtcagtttca tgcgtgataa tttggtctat atagatggtt
    10021 atagtcaagt gattaacggt agaaaacaga cagataacaa gttaaacgta gcctacgagt
    10081 taggagaaca agaggggcaa aaaggggcag aaatggttcg ccaagtcttg aaagataatt
    10141 ttgacttgga cattaagtac tacgccttgg ttgatttcca gtcctttgcc acagcgattg
    10201 acacgctttt ccctgatggg gtgacgattg atgctcaatt ttcaacattg aatgggcgtc
    10261 ccctaacaga agctacagtc ggagatgatt tatacgctac cgagactgag tctccaaccc
    10321 aaaccattaa agtcggaaaa cagcagatga atggctcgac cctgctcaat tatgctcgtt
    10381 tccgtgatga tgatgaggca gattacggcc gtacaaaaag acagcaacaa gttttaacag
    10441 caattttaga gcaaattaaa gatcccacta aacttttcac tggttctgaa gctcttggaa
    10501 aggtttttgc tatgacctca accaacgtac cctatacttt cctcctaaca aacggtttat
    10561 ctgttttgga tggagcaaaa aatggtattg aaaaattgac gattccagaa ctgggtgact
    10621 gggtagatgc ctatgatgtt tatggaggct tgggcttgct ggttgatcaa aacaaatatc
    10681 aaaccaagct cactcaaatg ggcttgagat aagataatac tcaatgaaaa tcaaagtgca
    10741 aactaggaag ctagccgcag gctgctaaaa acacagtttt gaggttatag ataagactga
    10801 cgaagtcagt tacctatacc tacggcaagg tgaagctgac gtggtttgaa gagattttcg
    10861 aagagtataa gacagaaaaa tcaaagtttg atagctattt ggatagcagt caggctttga
    10921 tttt
//
LOCUS       DAVKGC010000063        10908 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKGC010000063 DAVKGC010000000
VERSION     DAVKGC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.063"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            114..278
                     /locus_tag="VW049_001382"
     CDS             114..278
                     /locus_tag="VW049_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9432482.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            362..928
                     /gene="amaP"
                     /locus_tag="VW049_001383"
     CDS             362..928
                     /gene="amaP"
                     /locus_tag="VW049_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9432483.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            940..1110
                     /locus_tag="VW049_001384"
     CDS             940..1110
                     /locus_tag="VW049_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9432484.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1149..1757
                     /locus_tag="VW049_001385"
     CDS             1149..1757
                     /locus_tag="VW049_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9432485.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1788..1991
                     /locus_tag="VW049_001386"
     CDS             1788..1991
                     /locus_tag="VW049_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9432486.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2102..2374)
                     /locus_tag="VW049_001387"
                     /pseudo
     CDS             complement(2102..2374)
                     /locus_tag="VW049_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2683..2970
                     /locus_tag="VW049_001388"
     CDS             2683..2970
                     /locus_tag="VW049_001388"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9432487.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3248..4018)
                     /locus_tag="VW049_001389"
     CDS             complement(3248..4018)
                     /locus_tag="VW049_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU9432488.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(4033..4881)
                     /locus_tag="VW049_001390"
     CDS             complement(4033..4881)
                     /locus_tag="VW049_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU9432489.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4878..6353)
                     /locus_tag="VW049_001391"
     CDS             complement(4878..6353)
                     /locus_tag="VW049_001391"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU9432490.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6677..8032)
                     /locus_tag="VW049_001392"
     CDS             complement(6677..8032)
                     /locus_tag="VW049_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9432491.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGCFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(8042..8359)
                     /locus_tag="VW049_001393"
     CDS             complement(8042..8359)
                     /locus_tag="VW049_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9432492.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8376..8693)
                     /locus_tag="VW049_001394"
     CDS             complement(8376..8693)
                     /locus_tag="VW049_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9432493.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8708..9604)
                     /locus_tag="VW049_001395"
     CDS             complement(8708..9604)
                     /locus_tag="VW049_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU9432494.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9607..10869)
                     /locus_tag="VW049_001396"
     CDS             complement(9607..10869)
                     /locus_tag="VW049_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9432495.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFI
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
BASE COUNT         3758 a         2198 c         1691 g         3261 t
ORIGIN      
        1 aaattgattt tttgatatta aaataaaaaa ggagggtagg cagtgttgtg atcaattatt
       61 gctggaggtc ttattggtct cttggcaggt aaaatcacta aaaaagtagt tctatgggaa
      121 tcatcgcaaa tgtattcgct ggtttagtcg gggcatatgc aggacaatct cttttaggta
      181 gttggggtcc atcaatcgct ggaatggctt tgctcccatc tattgtaggt gcagcgattg
      241 tgattactgt agtgtcattc tttacaggta gaaagtaaac ttttcgccag taaagttagc
      301 aaactatttt taaatcaatg acgggaaaaa tagtttaaat gttaaatcga aaggattgta
      361 tatgtcaaaa gcaaagaaaa tatgtttcat tattttctgt attttaatct tgacaatttt
      421 ccttcctgtt ttgatagatt atcatcaagt tagtgatcta ggtattcatc tacttagctg
      481 gagacagaac tccgtagttg aattctatct tgctagatat gtcttttggg ggacagtggt
      541 tctatcaact ttagttttat tatccatttt agttgtgatg ttttatccta aacgttactt
      601 ggaaatccaa cttgaaacta aaaacgatac attaaaatta aagaattcgg caatcgaagg
      661 ttttgttaga agtttggtga gtgatcatag attgatcaag aacccaactg ttcatgtaaa
      721 tttacgaaaa aataaatgtt tcgttcatgt agaaggtaaa attcttcctt cagacaacat
      781 cgctgacaga tgccaaataa ttcaaaatga aataactaat ggattgaagc agttttttgg
      841 tattgagcgt caagtaaaac ttgaagttgc agtaaaaaat taccaaccaa aacctcaaaa
      901 caaaaagact gttagtcgtg tgaagtaagg aagtaaaaaa tggaatggct taaacaatat
      961 cgatatccaa ttatcgctgg tctcataggc gtatttctgg cttgtttgat tgtctccttt
     1021 ggcttcttca aaacaatatt tgtattgatt ttaggagcac tgggagttgc agctggatta
     1081 tatatcgaaa aaaactatat agataaataa aaaaataaaa attactaatt taattaaagg
     1141 agtttcatat gtcaaacgaa aaaaacacaa acactaacgt agaaaagaaa gatgctactg
     1201 ttgtagctca cgaaatcaaa ggggaactta cttacgaaga taaagttatc caaaaaatca
     1261 ttggtctttc actagaaaac gtttcaggtc ttttgggaat cgatggtggt ttcttctcaa
     1321 atcttaaaga aaaaatcgtt aacagcgatg acgtaacaag tggtgttaac gtagaagttg
     1381 gtaaaacaca agttgcagtt gacttaaacg ttattgttga gtaccaaaaa aatgttccag
     1441 ctttatattc agaaatcaga gaaatcgtat cttcagaagt tgctaaaatg actgacttgg
     1501 aaattgttga aatcaacgta aacgttgtcg acatcaaaac taaagaacag catgaagcag
     1561 actcagtaag ccttcaagat cgcgtatctg acgttgctga atcaacagga gaattcactt
     1621 cagaacaatt cgaaaaagct aaatctggtc ttggatctgg tttctcaact gttcaagaaa
     1681 aagttagcga aggtgtagaa gctgttaaag gtgcagcaaa tggtgtagta tctcacgaaa
     1741 acactcgtgt aaactaagat aaaataaata taacaggaga aattatcatg tcagtagaag
     1801 aaaaattaaa tcaagctaaa ggttctatta aagaaggtgt tgggaaagcc atcggtgatg
     1861 aaaaaatgga aaaagaagga gcagctgaaa aagttgtttc taaagtaaaa gaagttgccg
     1921 aagacgctaa agacgctgta gaaggtgctg tagaaggtgt taaaaacatg ttgagtggcg
     1981 acgataaata aggttaaaag ttactttatc ttttttggta acattagtca aaagagtctg
     2041 agtcaagatg attctcagaa aacaaaaagc tagagattcc caattgcgga actctagctt
     2101 tttaattttg cctctttctc ttattatatt tcagcaggtt gttggccatg agtatgaatc
     2161 ccatgtcaat tctcacttga cgcttacctc tcagatgaca tctcttataa cccaaacaaa
     2221 cctttatctg cccaaagaca gatttcatat caatcttacg tttagcgaaa atttgtctac
     2281 ccttggaaga taaaagtgcc tgatattctt tagtttttaa acactggtaa cgttcattca
     2341 tatacagtcc cttttgaggg gctgattcag gttcataatc gcagtcaaca ttgatttcaa
     2401 ggctgtttgc tttctatctc ccctttttca taatgtatag taaaatgaaa taataacagg
     2461 acgaatcgat cgggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtgtact
     2521 attctagttt caatctacta tattttcgta caggtgcttc aatagtttct atagtaaaat
     2581 gaaccaaaaa tagtacacgt tgcggtataa tgttcttgtg gcatattcat tagattttcg
     2641 taaaaagttc tctcctattt gtgagcgaac aggtatagtc aattgattgt cagtttgaag
     2701 accaagagtc gtaaggcaaa ggatatggca gaatccatcc aagggtggct ggcacaattt
     2761 ctagtgaacc tctttaaatc tattacgttt gactgtggaa aagagttttc aaaatggaaa
     2821 gatatttcca atcatcacga tagtgagagc ttttttgcga atctaggatg tccaagacag
     2881 cggtgtctca acgaacattc aaatagactt ttaaggtgtc atgacttgcc aaagcagaca
     2941 gatttcaacg aagtcagtca agaattttag gctgggtaaa aactcaattt caggagaaaa
     3001 tgaaataaat attcccacaa taaaacgcat agtatcaaag tttttccaac acctgatatt
     3061 atacattttt taattttaaa gactttttcc cagcctcaag aaatcacatc ccacctaaat
     3121 cctttggata ccaaatacct catcaggtat tttctgagtt ggtaagactg tctagcttaa
     3181 tttgacaaat ggagggcgtt aaatccagtt tttacaggaa gtctatttaa atattttcac
     3241 actgacctta caaaatatga aataaattat ataaagtcat tcccatgaca aagaataaca
     3301 tccctttaaa acagtatctt acccaatttt caggtaagac ttttgataca accgtcccca
     3361 atacccctcc gcatatagaa gcaagaaaaa taactggaac aggtgctaaa tgatactgat
     3421 ttaaacctgt aaccacaaca attgtagcca aatgagacaa ctgggaaaag aaaataattg
     3481 caagagaata catcgttgct tcttttatcg aaatagaaaa aaacaccatc aataaagata
     3541 cattgagcgg accaccacca atccctaaaa aactagaaat caagcccaat agaagaccac
     3601 aaatcatata gaatcctatc ttatcaaatt gataagtttt tttaaaatct gttaaaacaa
     3661 atgatacaag tagcatcaca aatagtaaaa tcatttgaac aatggatact agccgtactg
     3721 ataattgaga taacaaaact tgaaaaataa gatgacctag aaaccctcct aaaacagaag
     3781 aaaaggatac cgtaaaaatc aatctccatt ttatctcttt actttgtgaa aatcttttgg
     3841 ttgttgaaat aatcgccatt ataaaaacag agaaactaga ataaaaagca atatcactga
     3901 ccgaatggta tccaaaacta tccataagtg gcttaattat aattccacca cccatgcctg
     3961 atatagaacc caatccacta gctattataa tgattagaaa ataaataatc tcaaccactt
     4021 tttaagtcca tcctattctt tcaaacatct aaaacctaga tggctactag tggaagtaga
     4081 aatacatcca tttctagcag ctactcggta tcgattacaa taagaacaat gacataaaaa
     4141 ggaacctcct ctgatagcat actcctcatc ttgtatccca tattttggca actcatagtc
     4201 atcttcatta aaactagcta ataaggtata tctcggattg cgacaccatt cccaaacatt
     4261 cccaatcatt tgatatagac caaaatcatt aggtaaaaat tcatagacag gagccgttcc
     4321 tataaatcca tctaaggcag tattttcata aggaaatctt ccttgccaag tattggcatg
     4381 ataatatcca ccttctaaaa gagtatctcc ccaaggatat tcagattgtc ttcctcctct
     4441 agcagcatat tcccactgtg cttcagtcgg taaagacatt ccgctccaat tacagaaagc
     4501 caatgcatct tctaaagcaa catgaactac tggatggtct tctaaaccca ctagattact
     4561 attctcaccg tagggatgtt tccagcaagc gttcggaact tgtaaccacc acggagcccc
     4621 agcaggatga ggatatccct ccctttctgc ctctggaacg aacaatataa aaacaaaaga
     4681 ccactcttgt cgctcagcta gtgttttata gcctgtttct ttcacaaatt gagcaaattc
     4741 ttgattcgta actggagtat ccgcaatgga aaaatccttt acactaacag cgacttgagg
     4801 accttcaaaa tcatctaaaa agccctcttc agaatttgtt cccatcataa atgtcccacc
     4861 cttgattttt ttcattttca tgattgacta tcccccgttt tcgttagagt tggcgttatt
     4921 ttagaaagtt caacaggaac taactcttca tccacaacaa aaccttcttc tctatatcta
     4981 agaaagtcta ctaatttttt cttcatctga cgaacaatag gttggtattt ttcacttgga
     5041 tacaaatcat tcatttcatg cggatctttt ttcatatcaa ataactgata atgatttaaa
     5101 acaggaaacc agataaattt ccactggtct gtcaagatat attgactaga atcttttcct
     5161 aaagcatgtt caccatgaaa ttctgttcgc cacccctcgt actgtccaaa taataaattc
     5221 ttaacactcc gtccatctag ttcatctgta gtagttcctc ctgccaaatc cacgagtgaa
     5281 ggaaaaatat cttgaatttt cactaattgc ttgattgttc ccctattacc agctatcaat
     5341 ccagccggat cgtaaataaa cgaaggaatg tggatacttc cctgataagg atatcccttt
     5401 ctaaacagat aatgttcacc aagctgatct ccatgatccg aaacaaacca gataatcgta
     5461 tctttatcat gacgaaattc ttttagggca gtcaaaaagc gactaatttg atggtctata
     5521 tgagtaatca atccaaaata agcagcaacc attcttcttt gatcatcttc ctttaatttc
     5581 cctcttaaag cataaataga cggaatttgc ttttctaata cttcccaatt cccgatatgc
     5641 aaatcgagaa attgaggcaa gcgttccata tacatatcaa aatagtattt tgggggattt
     5701 aatggagcat gtggtttttc aaaagacatt ttgagaaaaa aaggaaccgt tggatctctt
     5761 ctttgtaaaa atgaaatgga ctcactcaca acccagtttg ttggatgtaa cttctcatct
     5821 ttatcccaag gtctagcttc ccaagaatta caatccattc cgtcatcaat caaatcaaca
     5881 tcataaccaa ctttaccctt taaaaaggct agatagtcac tagcataatc aaattgactt
     5941 ccataagcct tatcatactt tctatcaaca tgtagatacc catcgtgaag taagacatga
     6001 tcaaacccaa gtctctgtct ggaaggaaaa acatgcatct taccaataca ctctgtttga
     6061 taacccatat ctttaaagac ttttggaaga gtattagtaa aattccaagg cacctcatct
     6121 tgataaccaa ctcgtccact tttatcttgg tctaagccag tcaataaagc tgccctagca
     6181 ggaacgcaag aaggaactgg agaataggca ttttcaaaat tataacctac actagccatc
     6241 atatctaatg ttggagtgct aactagctta tccttactat tcaaggataa ggcgtctgct
     6301 ctcatttgat ctacaacaat caaaataata tttggctgtt ttgtctgaac cataaaatct
     6361 cctttctaat atggcaaaag aggcacaaga agatatctac ctttactgca cccctttcta
     6421 tatcaatctc tctatataaa acaataacat tcttgttatg ttttatagaa aaatgaacta
     6481 aaatatgact aaatcgatta ggaaattcaa atcattttct agtactattt tagaagttac
     6541 agtgtactat tccaacttca ataaactata aacctttgtc taataacaat tttagtggag
     6601 ataagaaatc ctacacctaa ctcaatctta cacgtaatct atttctattt tatcacaaaa
     6661 aacgcaagta agaccattaa ctcaattcag ttttatctgc cattttcaca aatgggaaat
     6721 aagtcaagac actaataatc aaacaaacaa ctgataagat gatggcacgc caatcaaatg
     6781 ctgtagagaa gcaaccatat aaaattggag gcattaccca agtaacattt tgtgtaacag
     6841 gtgaaacaag accccagctt gttgcccagt aagctaccgt tgccatgaaa accgggctaa
     6901 gtacaaatgg tataaatagc aaaggattca agacaactgg taaaccataa tttaccggct
     6961 caccaatatt aaacagaact ggtgctagac caagtttagc aacttttcga taatgactgt
     7021 ttcttgaaaa aattaaaata gcaagtacca atcctaatcc tccaaaccag acaaacgccc
     7081 caaaagaccc acttgtccat atataaggaa tcggttcacc tttttggaaa gcatccagat
     7141 tcgctaacat agcaactcca aacagccctt ccatgatggg agccaataca tttcctccat
     7201 ggagaccaaa aaaccagaat aacttattta aaaagatcat cagaataact gcaaagaaac
     7261 tttgagacaa acctagtaat ggcgtttgta acaccttgta aacccaatca atcaataagt
     7321 cattgctaag taaatggaaa acataagtca agatggctac tatatacatc gccataaatc
     7381 ctggaatgat agaagtgaac ggcttagcaa tcgcaggggg aactgaatct ggtaacttga
     7441 ttacccagtt ctttttcatt actttacaga aaataataga ggctaaaaat ccaatcatca
     7501 tggctgtaaa gtagcctctg gcattaatat ggtttcctgg aatcacattc ccaatagtta
     7561 ccatcagatt tttaccatca aatgctagat tatcaattcc ttgtaagatc tgatctaatt
     7621 tcacatctcc tacatttgcc aaagggaaac tctttgtaac tgtacttcca atcgaaatga
     7681 caaacgaagc aagtgatacc aaaccagcag aaactgtatc aaccttgtaa atcttagcga
     7741 tattcactcc caagcaatag atgaacaaca aggaaacaat tggtatactt cccttgaata
     7801 ccaaattatt gatgtcaaca agccactgaa aggttttcgt aatactttct aggtgaaatt
     7861 gttgtggtaa atccactaga aaagcattta ataacaaagc aatggaacct gtcataataa
     7921 caggcatagt ccccacaaat gaatcacgta aagcaactaa aaacttctga ttcccaactt
     7981 tcattgcaat tggagaaagt ttttcttgca ttttatccaa cattgtattc atactaaact
     8041 cctaattttt ttcaaatcgt tcgtatactt ctaccacttc tttcgccaag tcaacaaaag
     8101 tcagactcgt cattaaatga tcttgcgcgt gtaccatcaa gaggctaatt gatgtttctt
     8161 ccccctgtgc ctctcttgtc aacatttctg tttgagcctt atgcgcctgt aataaggcag
     8221 cattcgcatc agctaatctt cgattggctt tagagaaatc cccttttttt gctgcctgaa
     8281 tagcctccat agcattactt ttggcttccc caccatacat aattagcccc attgcagatt
     8341 ctaaattact ttcattcata gcattctcct caatcttagt ctaataaact ttcagctaaa
     8401 tctaagactt tagaaccatt catcattcca taatctgtca tcggaataac cgccacagga
     8461 atctgtctat cttttagttt ttcttgaaag tcccctagta aatagcgaac ttgagggcct
     8521 aaaagcaaca catttacttc ctttgttgct acaatttctt ctgcttcagg agctggaact
     8581 gcaaaaattt ctgcatccaa ccccttatct tctgctgcct tttgcatctt tgtcactagc
     8641 agacttgtac tcatacctgc tgcacatgct aacataattg ttactttagc catagcttac
     8701 tcctttatca cctatttact gacctagcca agcgagatac tgtatgtctc actcctctaa
     8761 ttgccttgct caatgcataa atattatcga ctgttgccag tcccccatat cctgcgtcac
     8821 ctatatgttg aatatcaact ccacaaattt tatttctaag tgcaatttcc ttgatagtat
     8881 ctgtatcaga tgtttcttgg ctagtaccaa tagcactcaa tacaagacca cccttactat
     8941 gaacgagatc aacgacttca cgcaactctt ggtcatgaaa ggctggaacg gttccaactg
     9001 ctggaacaag tatcacatcc gcacctgctt ccaacaattg ctctgcgaca gaaagctctg
     9061 ccacaggctc attcactcct gccccgtgca ttttaccagc tataatcaaa cctgagaaat
     9121 tctctttagc agattgaact gctttgataa tctcttgatt gctaactcct gttccaggat
     9181 ttccagtcaa acagacaaag tcaaaaccta actcctctat tcgcttcaat gtttcaacac
     9241 tagcaacacg acctgcaaca atttcctgtg tttcctctag catctttgca gatgggtcaa
     9301 tcggttccaa atttacacca attggacaag caacaagctg gtgtaagcga tgaataacat
     9361 catcacctga actatccaaa gcataaattt taggctcaaa aacatcaaca caattcaata
     9421 aaatcatatc agctccaaaa gctctagcaa tttcagaatt agttatatcc ccaacaaatg
     9481 tctcacgagt aactacattt tcagatagaa caactctacc ttcacttgct aaaatacttt
     9541 gttttaattc ttcagcattc atctgtaata tctcagatgg atttgcacta attaaacgtt
     9601 tcattctcaa tcctccaata acttaaaccg tttttcaata gctattacaa atggtaaata
     9661 aagtaagaca tccaaaacaa tcaatacaat ttgtaataat gaaccagaaa tacttcctgt
     9721 agcaagtaaa ccacttataa caggtggcat agtccaacct ggatctgtat aagttagcgg
     9781 aaccaaacct gatgccattg ctccccacgc tactagtaag ttaaacattg gagctaagat
     9841 aaatggaact agtaacaaaa tgttcaaaac aataggaacg ccaaacattg ctggttcatt
     9901 tatattaaat aatccaggta taacagttat aggtgccaaa gtttttagtt gtttactagc
     9961 tttcttctta tgtgctaaat atcctaacgc aagaactaaa cctatggttg caccaccgcc
    10021 acctatgaat acaaaattat cgataaatac gctagtaaaa atatttggaa gagtttctcc
    10081 agtttgataa gctactttat ttgcatctag attagctaac cataacggtt tcataactgc
    10141 attaacaaca tttcctccgt gtaatcctac aaaccaaaaa ctactattaa ggaaaattaa
    10201 gataagaaga ccaataacat tatttcctaa aagtccaagt ggacctccca aagctacttg
    10261 agcaatttca tgaagattag gtaaacttaa tttatctaaa gttgcataaa caatcaacca
    10321 accaacaata gtcactgcac caggaataat ggcactaaag cttttagata ctgctggtgg
    10381 aacaccatcc ggcattttta tctgtatatt atgatttata aaccattggt agatatagcc
    10441 attaatcaat cctaaaatca tagcaacaaa taaacctttt gatgccatga aagcggtagg
    10501 cattcctgct ccagcctctc ctgtaatgaa aggcgtaact gtaataaaag atgataatga
    10561 cacaatacca gcaggaatac catcaacttt gtattgtcta gccataaagt aagcgatacc
    10621 aaaactagct acaagaccaa gtaatccaaa actactatca acacccttcc ataaataatc
    10681 agcaacacca atatcaccca gatatttttc ccatcctgga ataggaaaac ttgcaataac
    10741 cattagtaat gaacctatca acaataaagg tatagttaga gtaataccgt ctcgaataga
    10801 aaccaacgct ttattattac ccaatctagc cgctactgga agtaactttt cttgtaataa
    10861 tttttccata accttttcct cttaaatata ttttattatc aaaagtta
//
LOCUS       DAVKGC010000064        10773 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKGC010000064 DAVKGC010000000
VERSION     DAVKGC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.064"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(31..1839)
                     /gene="glmS"
                     /locus_tag="VW049_001397"
     CDS             complement(31..1839)
                     /gene="glmS"
                     /locus_tag="VW049_001397"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9432496.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEKYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKRALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(2031..3410)
                     /gene="bglA"
                     /locus_tag="VW049_001398"
     CDS             complement(2031..3410)
                     /gene="bglA"
                     /locus_tag="VW049_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9432497.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYTRFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(3509..5362)
                     /locus_tag="VW049_001399"
     CDS             complement(3509..5362)
                     /locus_tag="VW049_001399"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9432498.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(5375..6634)
                     /gene="rseP"
                     /locus_tag="VW049_001400"
     CDS             complement(5375..6634)
                     /gene="rseP"
                     /locus_tag="VW049_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9432499.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(6656..7459)
                     /locus_tag="VW049_001401"
     CDS             complement(6656..7459)
                     /locus_tag="VW049_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9432500.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(7468..8226)
                     /locus_tag="VW049_001402"
     CDS             complement(7468..8226)
                     /locus_tag="VW049_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9432501.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKYLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEVALQEAILAYNRRHRRFGGV"
     gene            complement(8457..9029)
                     /locus_tag="VW049_001403"
     CDS             complement(8457..9029)
                     /locus_tag="VW049_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9432502.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(9010..9981)
                     /gene="ruvB"
                     /locus_tag="VW049_001404"
     CDS             complement(9010..9981)
                     /gene="ruvB"
                     /locus_tag="VW049_001404"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9432503.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDVITDKALTMLDVDHEGLDYVDQKILRTMIEMYGGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            10142..10234
                     /locus_tag="VW049_001405"
     CDS             10142..10234
                     /locus_tag="VW049_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9432504.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         3166 a         2506 c         2045 g         3056 t
ORIGIN      
        1 aaaaagccta gataactaga cttttttagc ttattctacc gttactgatt tagcaaggtt
       61 acgtggttta tccacatcga ggccacggtg gagggttgca aagtaagcga ctaattgcgt
      121 tggtacgacc attgaaattg gtgagaggta tggatgtacg gtcgtaagga cgatatcgtc
      181 ggtatctttg gctacattct cttctgcgat agtgaggact ttggcaccac gggctgcgac
      241 ctcttggata tttccacgag tatggttggc aagaactgga tctgacaaga gagccaaaac
      301 aggcgttcct tcttcaatca aggcaatggt tccgtgcttg agttctcctg ccgcaaaacc
      361 ttcacactgg atataagaaa tctctttgag tttgagactt gcttccatgg ctacgtagta
      421 atcttgacca cgtccgatgt aaaaggcgtt acgagttgtt tcaagaagtt cacgaacctt
      481 ggcttcaatg gtttctttct ctgaaagagt tgattcgata gactgagcta cgattgacaa
      541 ttcatgaacc aggtcaaagg cttgcgcttt agcattacca tttgcttctc cgactgcttt
      601 tgcaaggaag gcaagggctg cgatttgcgc tgtataggct ttagttgatg ccacggcaat
      661 ttcaggacct gcgtgaagga gcatggtata gttggcttca cgtgagaggg ttgaacctgg
      721 aacgtttgtc actgttaagc ttggaattcc catttcatta gccttgacca aaacttgacg
      781 actatccgct gtttcaccag attggctgat aaagatgaag agtggtttct tgctgagaag
      841 tggcataccg tagccccact cagatgagat tccaagttca actggtgtat ctgtcaattc
      901 ttccaacatt ttcttagaag caaatcctgc atggtaagat gttccagctg caaggatgta
      961 gatgcggtct gcgtcttgaa cagccttaat gatatctggg tctacgacaa cttgaccagc
     1021 ctcatctgtg taggcttgga tgagtttccg cataacagtt ggttgctcgt caatttcctt
     1081 gagcatgtag taagggtaag ttcccttacc gatatctgac aagtcaagtt cagcagtgta
     1141 gctagcacgc tcacgacgat ttccatcata gtcttgaact tccacactat cagccttgac
     1201 gattaccaac tcttggtcat ggatttccat gtattggtta gtttcacgaa tcatagccat
     1261 ggcgtctgag cagaccatat tgtagccttc cccaagacca atcaaaagtg gagatttgtt
     1321 tttcgctaca tagatgacat ctggattttc agagtcaatc aaggcaaagg catatgaacc
     1381 acggataata tgaagagctc ttttaaaggc ttcaagaact gagagaccgt cttcttccgc
     1441 aaattttcca atcaaatgta cggcgatttc cgtatctgtt tgccctttga agtggtgccc
     1501 agcaaggtat ttttctttaa tttcaaggta gttttcaatt accccattgt gaaccaagac
     1561 aaaacgttct gtctcagatc ggtgtgggtg agcattgtct tccgttggtt tcccatgagt
     1621 tgcccaacga gtatgtccga taccagttgt tccctcaaca ccagctgtct tggcagacaa
     1681 ttctgcgata cgaccaacag ccttcaccaa atggttatca gcaccatcta ggacaaaaat
     1741 tcccgcagaa tcatagccac ggtattcaag cttttcaagc ccttgaatca aaatatcagt
     1801 tgcatttgtg tttccaacaa caccaacaat tccacacata gtatatacga cacaggcaag
     1861 ctgtgctttc tccttaaaat tggtatagtc taattcatct tttatagaat cagcaaaaac
     1921 agtatatact tgtttctttc acttgtcaag agtaaaaatt ggtatagttc aaattaagct
     1981 cctgtaagca aaaaactctg accgattggg ataatcagtc agagtccttt ttaaaatcca
     2041 ttattatcgc ttaattcttt gaaccagtgg cctgatttct tcagacgacg ttcttgcgtt
     2101 tccaagtcta attcgaccaa accatagcga tttttatagc tgttgagcca tgaccagcag
     2161 tcaataaagg tccaaatcaa gtagccctta cagttggcac catcttcaat ggcacggtga
     2221 agttcacgaa gatgaccttt tacaaagtca atacggtaat catcttgaat cattccatct
     2281 tgacggaatt tttcttcccc ttcaacaccc ataccattct cagtcaacat ccactcaata
     2341 ttgccataat tttccttgat attttgggcg atgtcataaa tcccttgctc ataaatctcc
     2401 caaccacggt gagaattgat tttacgtcca ggcatcacat aaggctcgta aaaatgttct
     2461 ggtaagagtg gactctctgg atgcttagca aatcgaggag ccataacacg caaaggttga
     2521 tagtagttca caccaaggaa gtccaccgta ttatcacgaa tgagttccaa ctcttcctct
     2581 gtagcatcag gtaaaagacc gtgttcatgc aagatttcta ctaactcctg tggataagtt
     2641 cccaagacag atggatctaa gaaagattgg gcctgaaaaa gggccgcaat acgagctgcc
     2701 ttgacatcag caggatgctg gctacgtgga taagccggtg tcaagttgag gacaatccca
     2761 atcttggaat caggcaaaag ttcatggcaa gccttaacag cccggctgct ggccaattgt
     2821 gtatgatagg ctaccttaac agctgcctct gcatccacct tatgtggata atgggcatca
     2881 taaaaataac caaattctac aggaacgatg ggctcgttaa aggtaatcca ttgatccact
     2941 aaatctccat aagtctcaaa acaaaaacga gtatagtctt cataggctga gactgtcgcc
     3001 ttattttccc aaccatcacc atcctcttga agggcaaaag gtaaatcaaa atgatagaga
     3061 ttgactaaca gacgaattcc tttagcctta atagcctcaa agaccttacg ataaaaatcc
     3121 acaccttgag ggttgacttt tccacagcct tgtggaaaaa tccgtgacca ctgaatagaa
     3181 gtccgaaagg ctgtgtgacc agtctctaac aaaagctcaa tatcccgctc ccaattttca
     3241 taaaaagtcg atgtcttatc tgaaccaatc ccattatagt aacgatttgg ctccacttgg
     3301 aaccagtaat cccagagatt gtctccctta ccgtcaccag ctacacgtcc ttctgtttgc
     3361 ggtccagaag tagaggatcc ccagacaaaa tcctttggaa atcttagcat acatttacct
     3421 ctttatctac tcatttctcc cattatacag aaaaaacaag gtaaaaacta gttacatttt
     3481 ttccttgttt ttcttctgac tatagttttt atttcttgct taggatttca agcgtttcaa
     3541 gcacgttatc tgcatgaacc tcaatggtgt caccagttgc cttgatctta acttctacaa
     3601 tgccatcggc cgctttttta ccaacagtga tacggattgg aagaccaatc aagtcactat
     3661 cgctaaattt aacaccgaca cgttcgttac ggtcatctgt caagacttca taaccagctc
     3721 ccatcaagct tgcttcaagt ttttctgtca aggcttgcgc ttcttcatcc ttgacattga
     3781 cagtaatcaa atgcacatca aatggtgcca attctttagg gaaattgatt ccccaagcgt
     3841 aacggtattc accttttggc gttttgttaa caaagaggcg agcgtgttgc tccatcactg
     3901 ctgaaagaag acggctgaca ccgataccgt aacatcccat gatgattggc acagcacgac
     3961 cattttcatc caagacatct gctcccatgc ttgctgaata gcgagttccg agtttgaaaa
     4021 tatgaccgat ctcaatacca cgcgcaaagt taaggacacc ttgtccatct ggggaaattt
     4081 caccctcacg aacttcacgg atatccacat attctgcagt aaaatcacgg cctgggttca
     4141 caccagtcaa gtggtagcca tcttcgttag caccgacaac tgcattgcga acatcttgca
     4201 ccttacgatc tgcaataatt ttaatattct ctggcaaacc aactggtcca agtgaaccaa
     4261 atcctgcttg aacaacattc gccacttctt cttcgctagc aacgtcaaag aaatctgctc
     4321 ctaagtggtt tttcaacttg acttcgttga gttggtcatt tccaactaga agggctgcaa
     4381 caagctcacc atctgcaatg tagaagaggg ttttaatcgt ttgttcttct ggaacattga
     4441 ggaaggctgc aacttcatca attgatttaa catctggcgt tgcaacacga gtaacttctt
     4501 cttcagcgac aacacggttg cttggtttgt actcgtttgt tgccatttct aagttagctg
     4561 catagctaga ctcacttgag taagcaatgg tatcttcacc agagactatc catttgagca
     4621 attctgcctt gatttcttct tgcacttctg caggaatttc gtcaaatgag gcaactgact
     4681 tgtccaagac aacccagcgg tcaaggtctg tacgagcaga tgtaatggcc ataaattctt
     4741 ggctatcctt accacccatg gctccaccgt caccaataat agccttgaag tctaaaccac
     4801 tacgagtgaa aatacgctca taagctgctt tgtactcatc ataaacacta tccaaactat
     4861 catagttagc gtggaaacta taagcatcct tcatgataaa ctcacgtgta cgaagaagtc
     4921 cattacgcgg gcgtttttca tcacgatact tgggctgaat ttgataaagg ttgagtggca
     4981 attgcttgta agatttaaca gaatcacgga caatagctgt aaaggtttct tcgtgagttg
     5041 gacctaagat aaagtctgat ttttcacggt tttttagttt gtaaaggtct tcaccatagg
     5101 tttcgtaacg acctgattca cgccacaatt ctgcactaag aagggctgga gccaacatct
     5161 caacagcacc aatcttttcg aattcttggc gcatgatgtt tttagctttt tcaatcacac
     5221 ggttggcaag tggtagataa gaataaacac ctgctgaaac ttggcgaaca taaccagcac
     5281 gcaacataag agcatggctg ataacttgag catcgcttgg catttcgcga agcgttggga
     5341 taggcatttt actttgtttc ataatattcc tcgattatct aaaaaagagt cgcataatgt
     5401 cattccaagt cacagcaatc atcaagacaa ccatgatgac cactccggcc aaggtgacat
     5461 aggtttcaat ttcttgtttc aatggtttgc ggcggatggc ttctaggata ttgagcacaa
     5521 tcttaccacc atccaaggct ggaatcggaa taagattaaa aatcccaata ttgatggaaa
     5581 tcattgccaa gaagtacaag atattctcaa ttccattttt agcagcatca ctacttgcct
     5641 taaagatagc aacaggtcca cccaacttgt tcaaatccgg ttggaaaatc agatttttca
     5701 gagctgagag aattcggaga gctgagtcag cagcagttgt aaaaccacct acaaacatgg
     5761 atagaaaatc tgacttaacc cccggttgaa cacctagaag gtaacgacct tgactatctt
     5821 cgggtgtaac agtgacttgt ttgtcactcc ccttttcaga aatagtcaca tccaaagtcg
     5881 gtgccgtctt atctttggtt tctgtttcca cagcttggat caagctttcc cagttgctaa
     5941 cctcatgtga gccaatcttg gtaatttgtg ccgtttctgg tactcctacc ttggccaagg
     6001 caccttgggg catgatatgg aactgattgg tatcaacatc tctgacacca ccctgcataa
     6061 agattaaaac ccaaaaaaca acgacaccta agataaaatt gttcatagga cctgcaaaat
     6121 tggtaatcag tttgccccag atagtcgcat tttgatattg aacatctaaa ggtgcaatcc
     6181 gaacctcagt accatctgct tccacaaccg ttgcatcgtg atccactgca aatgtttttt
     6241 cttcttccag aaccaatcct ttgataaaga gcttgtcttc aaaatcaaac tgggtcacct
     6301 gcatagggag ggctgtttga tccaattttt tacctgagag attgatgcgt ttaaccttac
     6361 catcatcagc aagtgtcaaa ctaacaggcg ttcctgtctt gatttcagtt gtatcatcac
     6421 cccaaccggc catgcggaca tagccaccca gaggcaagat tcgaatggta taggccgttc
     6481 catccttgcc aatgtgagca aaaattttag gtcccatacc gatggcaaat tcacgtacta
     6541 aaatccctga tttcttggca aagtagaagt gcccgaactc gtgcaccact acaataatcc
     6601 cgaaaaccag aataaaggtt aaaattccga gcatagcgtt tcctccgtct tttgattaaa
     6661 agagtccaaa taagtgcatg attggaaata caagcaacat actatcgaaa cgatccaaaa
     6721 caccaccatg tccagggata aatttcccag aatccttaac accaaaatga cgtttgatcg
     6781 aactttctag taaatcacca aattgtccag caatgctaaa gaaaatagca aagactgaca
     6841 tcttgtaaat tccatatgga agagcaactg tactgtcaac tatcataaag ataatggtta
     6901 ctaaaattgc tcctaaaata ccacccaagg caccctcaag ggttttatta ggcgataccc
     6961 ttggtgctaa ctttcgtttc ccatagttca tcccaacaag ataggcacca ctgtctgtcg
     7021 cccagacgat acacaaggct aagagagcct tgtccaaacc tgcaacacga gcatctagta
     7081 aagcattaaa tccaaagccc acgtagaagc tcatagcaag agggaaaacc gcatcctcaa
     7141 tcgtataaga cttgctaaaa acggtcgttc ctaacatgat tgaaatcaaa acactatagg
     7201 caaccacatt cccatcaact ggcaaaaaag tcaggtaatt ctccaaggga atggtcaatg
     7261 caaaggttgc aaagagggtc aagaggccct ccatcgtcat ggtctctaga cctctcatct
     7321 tcaaaagttc atgcatggct agcatggcta tgattccgat tgctatctga agcaagaggc
     7381 ccccaatcat taaaattggt aggaaaatag ccagggcaat ccctgcaaac aaggttcttt
     7441 tctgtaaatc ctgggtcata tttcctccta aactcctcca aatcggcgat gacgacgatt
     7501 ataggcaaga atagcttcct gcaaggccac ttcgtcaaaa tcaggccata aggtgtccgt
     7561 aaaataaagc tcactatagg ctccctgcca tggaaggaaa ttgctcaaac gtaattctcc
     7621 actagtacgg ataatcaagt ctgggtctcg taagtactta ggcaaatgct gggtaaagag
     7681 atagttgcca atcaattcct ctgtgatgtc acctgggttg attttggcat ctaaaacatc
     7741 ctgggaaatc aacttaagcg cctgtgtaat ctcagcacgt ccaccatagt taagagcaaa
     7801 attaagaatc aatcctgtgt tgttcttagt caattcctca gccttggtta aagcttcaaa
     7861 ggtttgctta ggcaggcggt ctgtctcccc aatcatttga atcttaacat tattcgcatg
     7921 tagttccggg acataattat cataaaactc tactggcaag ttcatgataa acttgacttc
     7981 ctgatctgga cgggtccagt tttccgtaga aaaagcatag accgtaataa ccttgacgcc
     8041 cagtttgttg gctgccttgg tcacggtttg caatgcttcc atgcccgcct tatgtccaaa
     8101 aactcgcggt tgcatacgtt ttttagccca acggccattg ccatccatga tgatgccgat
     8161 atgagcagga acctgtgtcg gaacctctac ttccacagcc ttatctttct taaaaaatcc
     8221 aaacatgatc ttattcctat tcaaaaatct atcgtttcat tataccatat ttccccattt
     8281 tcttctatca ctaagctatt tattctcagg caccaagccc atttttcaaa aaaataagcc
     8341 gcctgattgg gcgactttat ttttataggg agattattat gaaaaagttt taggagttta
     8401 agttaaggtc ttcttaactt atgaacttag tgtacactcc ctagcttaaa gtttccttaa
     8461 gtatttttaa aaatcaaatt tttccatttc tcctgccaat ttttcttgga taaacgtgtt
     8521 tgatagagtt ccattcggtc ttcattttct aagaaatgag gagttggacg aacttgaaaa
     8581 ttcaaaatat cctccaaacc ataaggtgca tagagttcaa aatctgattc ttcattcaag
     8641 cgcagtccaa ctgtcgtaca ccgttctgga tacttactca tagcatcacg agaactggta
     8701 taggaagcag tgtgaggact gtgctgatgc atatagacct gatttttcaa ttcccactgg
     8761 tactgaggaa aatcctctcg cagctttttc tccagtaata aggtttcctc ataagaaaaa
     8821 tctggatcaa agaaaatcac atctatatct gtttcatgat caaaagggga tttgtctgac
     8881 aaaagattcc aaatgaaatt tctgacagaa cctgctgcca accacgagtc tttcagacca
     8941 aggtctcgga tgatcgtcag aatggccatc atatctggat tttctctaaa agcctctaag
     9001 atttcttgct tatttttcac tgtattcata acctaagtgc tcatatgcct tagcagtcgc
     9061 cacccgtcca gaccgtgtcc gcatgataaa acctttttga atcaagtaag gctcatacat
     9121 gtcttctaca gtctcacgct cctcggcaat attcacagaa agagttccta atccgacagg
     9181 gccaccaccg tacatctcaa tcatggtgcg aaggattttt tgatccacat agtccaaacc
     9241 ttcatggtca acatccagca tagtcaaagc cttatcggta ataacatcat cgataacccc
     9301 attccccatt atctgggcaa aatcgcgcac gcgcttgagg agacgattgg caatacgagg
     9361 ggtcccacga ctacgtaggg ccaactcaga tgctgcctca tgggtgattt ccatctcaaa
     9421 aatatctgcc gtccgctcga caatttctgt caaatcagca tgagcataat actccatatg
     9481 acctgtaatc ccaaaacgtg cccgtagcgg attggagagc ataccagccc gagtcgtcgc
     9541 accaatcaag gtaaaaggtg gtaactccaa atgaacacta cgactaccct caccagcccc
     9601 aatcataata tcgatgtaga agtcctccat agcactataa agcacctctt ccactgacat
     9661 tggcaaacga tggatctcat caataaaaag cacatcccca ggctctaact cattcaaaat
     9721 agctaccaga tctccggctt tttcaatgac tggacccgac gtctgcttaa gattgactcc
     9781 cagttcgttg gcaataacaa aggccatggt cgttttcccg agacctggag gcccaaataa
     9841 gagcacatga tccagcgctt catcccgcat tttggcagct tcaataaaga tttgtagctg
     9901 gtccttgacc ttatcctgtc cgatatattc acgtaaatac tgaggacgga gcgtgcgttc
     9961 tactaactcc tcatccccca ttatctcatt atctaaaatt ctactcatgg ctctattata
    10021 tcaaaaaacc aagccacaaa caaaaaagcc acctgattgg gtgactccta agtttagcac
    10081 ttatgtggta taatattcta cgacactgct acaccgccta cgaaaggagg tgagatagcc
    10141 catgatggaa ttagtactca aaactatcat cggaccaatt gtggtcggtg tcgttcttcg
    10201 tatagtcgat aaatggctaa acaaggacaa atagtgtcaa aaaagacctc aagcttattt
    10261 ggtcgtgagc ttggggtctt ttctagccta tgatatagaa ctagtactca attccttttt
    10321 attatcccat agttcacgaa ttttgtcaaa ggtttacatt ttcttcaacc gctgtacgac
    10381 aagacggtta agattaagag aacgttaggg attctatcaa tttcatagaa attttgattt
    10441 cgtaaacgaa gagacaatct tacatgtcac ttctcattta atacgccact actagacaag
    10501 caaaatcatt attacagtag ttccagttct tcaattaaca gtcacttaca atcaaattga
    10561 gtttgaacta gctgaagcga ccacagacct atttcttagt tatattcgct aaaaaagtcc
    10621 ccgccaattc cccgaccaaa atccgaaaaa taccgaaaaa tatcgaaaaa ttatttttag
    10681 aatagtcccc aaaagcctga aatagagcta aaaaactcca cctgattggg tggagttaag
    10741 ggagattatt atgaaaaaga aaagtttagg att
//
LOCUS       DAVKGC010000065        10713 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKGC010000065 DAVKGC010000000
VERSION     DAVKGC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.065"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(58..1380)
                     /locus_tag="VW049_001406"
     CDS             complement(58..1380)
                     /locus_tag="VW049_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9432505.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMVLPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(1471..1559)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1723..4059)
                     /locus_tag="VW049_001407"
     CDS             complement(1723..4059)
                     /locus_tag="VW049_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9432506.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYTNLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4163..4711)
                     /locus_tag="VW049_001408"
     CDS             complement(4163..4711)
                     /locus_tag="VW049_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9432507.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4708..5010)
                     /gene="zapA"
                     /locus_tag="VW049_001409"
     CDS             complement(4708..5010)
                     /gene="zapA"
                     /locus_tag="VW049_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9432508.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5097..5978
                     /locus_tag="VW049_001410"
     CDS             5097..5978
                     /locus_tag="VW049_001410"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9432509.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANCFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5982..6596
                     /gene="lepB"
                     /locus_tag="VW049_001411"
     CDS             5982..6596
                     /gene="lepB"
                     /locus_tag="VW049_001411"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9432510.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            6676..6798
                     /locus_tag="VW049_001412"
     CDS             6676..6798
                     /locus_tag="VW049_001412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HEU9432511.1"
                     /translation="MKIKEQTRKLAVGCLKQCFEVVDRTDEVSSKYCFEVADGS"
     gene            6875..9241
                     /locus_tag="VW049_001413"
     CDS             6875..9241
                     /locus_tag="VW049_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9432512.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(9289..10572)
                     /locus_tag="VW049_001414"
     CDS             complement(9289..10572)
                     /locus_tag="VW049_001414"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9432513.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQSLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         3054 a         2435 c         2193 g         3031 t
ORIGIN      
        1 atcaggctga taaaaattat ttataggcaa aaaaatcaca cgagctgtgt gattccatta
       61 tttgtcaaaa tactttttag tttcagcaat aacgactggc gacaagacca agagggcaat
      121 caagtttggc agaaccatca aggcattaac gatatctgcg ataatccaga ccatatccaa
      181 ctcgataaat cctcctaaca agaccatgag cacaaaaacc acacggtaga gccagataaa
      241 gcgaacccca aagaggaact caaaacagcg ttctccgtaa tagttccaac ctagaatcgt
      301 tgtaaaggca aaaagtacaa ggaagatggt caagagggca ggcccaaagt gtgaaaagac
      361 tgttgagaaa gctgactgag tcaaggcaac cccattcaag tcaccactcc aaactccagt
      421 taccaagatg gtcaaaccag ttagagtaca aatgatgagg gtatcaataa aggttcctgt
      481 catggaaatc aaaccttgct ctactggttc atttgtcttg gcagctgcag ctgcaatagg
      541 agcagaaccc agaccagatt cgtttgagaa cacaccacgc gccacaccat tttgaatagc
      601 catccgaacg ctagcaccag caaatccacc taccgcagca aggggactaa aagctgaggt
      661 aaagactaaa gcgattgtgc cagggatttt tccgatatta aagaaaataa ctgtaagagt
      721 tcctaagata taaatgatgg ccataaaagg aacaacagta gttgaaacct tagaaataga
      781 cttgagtcca ccaaagactg caatcgctac aaagacagac aagacgagag ctgtgatggc
      841 tggcgaaatc gtcgttgtat tttggataga ttctgcaatc gagttgactt gggtgaaggt
      901 tccgattccc aagagagcaa ccaatactcc tgctactgca aacaaaacag caagtggtcg
      961 ccacttttct cccatcccta gaaggatata gtgcatggga cctcccgcta ctgcaccatg
     1021 gtcgtccttg gtgcggtatt tgatggccaa gagtccttcc gcatacttgg tagccattcc
     1081 aaagaaagcc gccatccaca tccaaaatag agctcctggt ccaccaacct tgatagccgt
     1141 cgcaactcct atgatatttc ctgttccaac agttgatgcc aaggctgtac acagagctgc
     1201 aaaactggat acatcaccat gtcccttatc ctggataaaa ataagctgaa aggccttggg
     1261 cagacgcaaa acctgcaaga gtcctagccg catggttagg taaatccctg ttccgaccaa
     1321 taaaatcaag aggggcggtc cccaagcaaa agcatcgatt gatttaagca attctaacat
     1381 tttcttctcc tatcgtttca accccaaaag aaagagcaca tgcaagatac atgtactctg
     1441 gaatgcttag ataaatgcta aaaagcggtc tatcctagct ctgtcctttt acctgagagt
     1501 ttgagcagtt gcctgccttg ccccttcggt gcctttacgg tctctccaga gttccgtcca
     1561 tttacagtca tggaaaatca aacgattccc cacttctatt aaacttcatt cggtgttggt
     1621 atttaattga ttctaatttt acaaaaaatg ttggcttttg tcaatgtgtt tattagtaaa
     1681 aattagttca acagttttta ctttataaag tccagaatac tgctatcctt taaaagtgac
     1741 aatagtcgca ccactgcctc cagcattttg tggggcatag ccgaaactct tgacatgttt
     1801 gtttctttgc aagtatttgg taactccttc acggatgact cctgttccga taccatggat
     1861 gatatcaact tgagccatat tgttaagcaa ggcttggtcg atgaaggtat ctagctcatt
     1921 catggcttct tcatagcgct tgcctcgaag atccagtcta gcttgaggtc ctcgcccaga
     1981 agttcgtttc acaacattga cctgtttctt cttgactggt ttttcttgct gggcttgaac
     2041 aagatcaaac tctttctctt ccaaggtcat cttaatcaag ccaacttggg cttcccagcg
     2101 accgtccttg agttgactgg tcaaggtacc acgctgacca taactgagaa ccacgatatc
     2161 atctcccacc tttggagctc gttttttctt ggccttttga aggaccttat ttttagacaa
     2221 gtccactttt tcaggagcca attttttcaa cttggccttg gcttcaatga tttcgtgggg
     2281 cttgagttgg gatttactgt ggagattttt gagaatctgg tcactttcac ttagggccat
     2341 atccacaatc tcagcagcct gttcacgcgc cttgttaagc tcggtttcct tttcacgatt
     2401 aagctcgttg tagagttttt ttagcgcacg gttcatcttg agattttctt gctccacctc
     2461 acggatattg tccaagcgtt tgcggctttc cagcgtctgc tcttctaatt gctcaatgat
     2521 acgattgacg tcattgtcct gatcgatctg ctgactggca tctcctacga taacttcaga
     2581 taggcctaga cgtttggcaa tttcaaaggc attacttcgg ccaggaacac cctgcataaa
     2641 gcgataggtc gggcgaagag ttgcagtatc aaactccata ctggcatttt gcacaaaggc
     2701 tgtctcaata ccgtaggcct tgagttctgg atagtgggtc gtcgccatgg tcttgatttg
     2761 acgcaggcga aggtcctcca gaatagccat ggcaagggct gctccctctt ggggatcagt
     2821 accagccccc aactcatcca aaagtaagag tgaatgttgg ttgaccttgc caagaatatc
     2881 cacgatattg gtcatatggc tagagaaggt agacaagctc tgctcaatag actgctcatc
     2941 tccaatatca gcaaagattt cttcaaaaat accaacacga cttcccttgt ctgctaaaat
     3001 cggcaatcct gactgggcca tgacctgtgt caagcccaga gttttgagca tgatggtctt
     3061 cccacctgta ttgggacctg taatgacaat agctgttaaa tcttgaccaa aatagacatc
     3121 atttgcgacg gcatttttga ccaaaggatg gcagacatgg agcagttgaa tctcttgatt
     3181 ttctgacagc tgaggcacga ctgcttgtct ttcttggata aatcgaacct tggcacgaat
     3241 caagtccaga tgaccgataa tccaagcgtc attagcaatc tcagccgcat gagggcggac
     3301 acgctcagaa atttcttgga gaatgcgaag catttcatag cgctcatctg ctcgcagact
     3361 agcaatttct tcgctcagtt tgactacctc acgtggttcg atatagacgg tgtttccact
     3421 agcagaaata tcatgaacga cacctgcaat cttattgcgg taggtgtttt tgactggtaa
     3481 aacctgacgg ccatttctgc tagcaacaat tccttccgtc aacatctgcg ctttttgctt
     3541 gagcaagtct tgtaaaacat cgcgtacctg actctcgcta tcatgtattt ttcgacggat
     3601 tcgcgccaat tcttcactgg caaaattttc aatgaaaccc gcatcattaa aggcctgaag
     3661 atttccttgt aattgcggaa aatcatgtaa tttctcaaac caaagggcta attcttccaa
     3721 gctgacattt tccagattgg tgtaaaaatt ttgaagttct cggctggcaa gaagcacgcg
     3781 tttcaagagt aggaactcct cgatattgag atccgctccc atctccaacc tcttgcagac
     3841 tcctgcaatt tccttagttg agagaatagt aaaatgcggt tgctcgacga aaagagcctg
     3901 catttccttc atctcagcaa aagcctgttt gattttatct gctttggcag tcggagccag
     3961 ttgtctcaat tgctccaagc cctgctcggt caacaaatga ggctcaaaca aggccttgac
     4021 cttatcgaac tctaatgttt ctaatatttt cttattcatc tttatttcct tgtctttgaa
     4081 tgtctaggtg tggtagcttt ttacattctg atagattcga gtcaaatctg taaacaaagg
     4141 gctaggaaaa ctcctgccct ttttatccga ttaaatttgt cacccagatt tgtttgagcc
     4201 aactggttgt tatcggtatg ctctggatga tgtgttttgc gacgatactc ttttcaagag
     4261 gattttgtat aactgccatg gggatggtcg ccaagattgt caaggccatt tgcaagacaa
     4321 ataaggtcac caacatggac aagatacctg ctgaaacttg gaacaactta ccacccagtt
     4381 ttttgctagg aatcaagtgt aagagaagac caagcaaacg accaatgcta tagacaatcc
     4441 caaatacaag caagtagccg atacctgcat aaaagacctt atccagctga aagagttgat
     4501 ccgatgggaa aaagaaagtc ccctgacctt cctgcgaatt tgcataaggg agcaataaat
     4561 ggaattgctc tccaagcccc ttataaaact ggccagccat aaaagccgat gccatggctg
     4621 aaatcaggta ataaacctgt aagagcaggc ctctccgata gccgatataa aatccccaaa
     4681 ccaagaccaa tagaagaagg aatgaaatca taaggaatcc tcaatcttgc tctgttcttg
     4741 cttacaagtc acaagcttgt gacggagttc ttctagctct tgctccttat cgtcaaattc
     4801 aatctcacgg ctgagctgag ttgataaaca gttgactgcc aacaaaagag cgattgtttc
     4861 atcatctgcg ctaggcattt gttctttaat tgcttggtat ttttctgtcg caaccttagc
     4921 gatttcctcc ataaaaaggt tatcatgttc gcttgtcaag gttaacgatt ttttcccgaa
     4981 tgtaaatttg aatcgattta gatttgccat aaaattcacc tcacgatatt ataccaaaat
     5041 tcgctaattt tgtcagtttt tacaaatttt actgcttttg tggtacaata gaaactatgg
     5101 caagtatcac actcacacca agcgaaaagg atattcaggc ttttcttgaa cactatcaaa
     5161 ccagtctggc tcccagtaag aatccctata tccgctactt tttgaaacta cctcaagcaa
     5221 cggtttctat ctatacttct ggaaaaatct tgcttcaggg tgaaggggct gagaaatacg
     5281 ctagtttctt tggctatcaa cttgtagagg aaaacagggg gcaaaatctt cctttgattg
     5341 ggacagatga ggtagggaat ggttcctact ttggtgggct tgcagttgtg gcttcctttg
     5401 tcacacctga ccagcatgcc ttcttacgaa aacttggtgt gggagattct aagactctga
     5461 ccgaccaaaa gattcgtcag attgctccta ttctcaagga aaaaatccag caccaggcac
     5521 tccttctctc acccagcaag tacaacgagg tcatcggaga ccgctacaat gctgtttcgg
     5581 ttaaggttgc cctccataat caggctatct atctcctcct tcaaaaaggt gttcagcctg
     5641 agaaaattgt gattgatgcc tttaccagtg ctaaaaatta tgacaagtac ttggcacaag
     5701 aagccaattg tttcagcaat cctatcagct tagaagaaaa ggctgagggc aaatacttgg
     5761 ctgtcgcagt ttcttctgtc attgcgcgtg atctctttct ggaaaatctt gaaaatttgg
     5821 gacgagaact gggttatcag ctcccaagtg gagctggaac ggcttctgac aaggtggcta
     5881 gccagatttt gcaagcctat ggtatgcagg gactcagctt ctgcgccaaa ttgcacttta
     5941 aaaacactga aaaagcgaaa aaacgcttag aaaggtaagt tatgaattca tttaaaaatt
     6001 tcttaaaaga gtggggattg ttcctcctaa ttctgtcatt actagcttta agtcgtatct
     6061 ttttttggag caatgttcgc gtagaaggac attccatgga tccgacccta gcggatggcg
     6121 aaattctctt cgttgtaaaa caccttccta ttgaccgttt tgatatcgtg gttgcccatg
     6181 aggaagatgg caataaggac atcgtcaagc gcgtgattgg aatgcctggc gacaccattc
     6241 gttacgaaaa tgataaactt tacatcaatg acaaagaaac ggacgagcct tatctagcag
     6301 actatatcaa acgcttcaag gatgacaaac tccaaagcac ttactcaggc aagggctttg
     6361 aaggaaataa aggaactttc tttagaagta tcgctcaaaa agcccaagcc ttcacagttg
     6421 atgtcaacta caacaccaac tttagcttta ctgttccaga aggagaatac cttctcctcg
     6481 gagatgaccg cttggtttcg agcgacagcc gccacgtagg taccttcaaa gcaaaagata
     6541 tcacagggga agctaaattc cgcttctggc caatcacccg tatcggaaca ttttaagaga
     6601 cctaagaggc cgagaatcac caatctcagc ctcttcttct atcgtgagaa gtgattgttt
     6661 attgacaaat actcaatgaa aatcaaagaa caaactagga agctagccgt aggctgctta
     6721 aaacagtgtt ttgaggttgt ggatagaact gacgaagtca gctcaaaata ctgttttgag
     6781 gttgcagatg gaagctgacg tggtttgaag atattttcga agagtataaa ataaaaacaa
     6841 tcccaactct cacctattca tgcaaaggaa ttttatggaa gtttattttt caggaaccat
     6901 tgaacggatt atttttgaaa atcccagcaa tttttatcgc atcctcctcc tagaaatcga
     6961 cgatacggac gcagaggatt ttgatgactt tgaaatcatt gtcacaggaa ccatggctga
     7021 tgtaattgag ggcgaagact atactttttg ggggcaaatt gtccagcact ccaagtatgg
     7081 agaacaactg caaatcagtc gttatgatcg cgcaaaacca actagtaagg gcttggtcaa
     7141 gtacttttca agtagccatt tcaaggggat tggtctcaag acagctcaga aaatcgtgga
     7201 tacctatggc gaaaatacca ttgacgaaat tttgcaacac ccagaaaagt tagaaggcat
     7261 cgcaggactc tctgccaaaa atcgcgaggc tttcgtctcc actctccgtc tcaactacgg
     7321 aacggagatg gttttggcca aactagccaa ctacggcatt cccaacaaac tagcctttca
     7381 gattcaagac ttttacaagg aagaaaccct tgatgtggtt gaaaattatc cctaccagct
     7441 ggttgaggat atcaagggtt tgggctttac cattgctgac caactagctg aggaactagg
     7501 catcgaaagt caggctcctg aacgcttccg cgccggtcta gttcacagtc tttttcaggc
     7561 ctgtatggaa acaggggaca cctatgttga agcacgggat ttgctggaac aaacccttac
     7621 tctccttgag tcttcccgtc ccgtagaact agaccccagc caagttgccc aagagctctc
     7681 ctacctgatc gaagaagaca aggttcagca gattgatacc aagatctttg acaacagcct
     7741 ctttttcgct gaggaaggca tccgcagtca cttggttcgt atccttgaaa aaggaaaaca
     7801 gaagagtcat gatttagaaa ccattcaaaa acatatcact actatcgagg aagagctggg
     7861 aattgagtat gataacattc aaaaacaggc tatctgtgat gctatccaga ataaggtctt
     7921 tatcctgaca ggtggacctg gtactggtaa gacaactgtt atcaatggga tcatcgctgt
     7981 ttacgccctg ttagagggac ttgacctcag gaagaaaagc aatctgccca ttcttcttgc
     8041 tgctccaact ggacgagccg ctcgccgcat gaatgaattg acaggtttgc ctagcgcgac
     8101 catacaccgc cacttgggaa tgacaggtga cgatgatacc agtcatctgg aagattacct
     8161 agatgctgac tttatcatcg tggatgaatt ctctatggtg gatacttggc tggccaatca
     8221 actcttctcc aatatctctt ctaacagtaa gatcctcatc gtaggtgaca gtgatcagct
     8281 accttctgtc agtcctggac aggttctagc ggatctgctt catattcctt tgattcctca
     8341 gactcgcttg gaaaaaattt atcgacaaag cgaagaatca accatcgtca ccctagctag
     8401 tcagattcga cagggcatct tgccagctga tttcacccaa aaaaaagctg accgttccta
     8461 ctttgaaatt gctagtggcc atattcctgc caccattgaa aaaatcttag gtgccgccct
     8521 cagaaatggt attcctgccc gtgatatcca agttctggct cctatgtacc gagggacggc
     8581 agggattgat gctatcaatc agctcatgca agacctgctc aatcccccac aaaaagatca
     8641 actcagtttt gaagctcctc agtgccacta tcgtaaggga gacaaggtca ttcatttggt
     8701 taacgatgct gaaatcaatg tctttaatgg ggatttagga gctatcacag acctgattcc
     8761 tggtaaatac accgaatcga aacaagacga gattgtcatt gattttgatg gtaatgaagt
     8821 ctcttacccc cgtaacgaat ggtacaagat tcgcttggcc tatgccatga gtattcataa
     8881 gtctcaggga agtgagttcc cagttgtcat cctaccgatt actagtgcta gccgacgtat
     8941 gctggagcga aatctcatct acacagccat tacacgtgcc aaaagcaaac tcatcttact
     9001 aggcgaatta caagccttcg actatgctac ccaacacatc ggaactgccc gaaaaaccta
     9061 tctgattgaa cgcttcagtg atctactgga gaatgttgaa gaaaagcaac aagctgtctc
     9121 tgaaacagtc acatcaagtg cctctgaaca atcctacatc ctaaccgaag aaaactggga
     9181 cagcattcca gccatgattg ggattacaga cacagacctc aaagagattt ttggaaaata
     9241 gtacatcaga aaacccacca attcaggtgg gttttctgtt tattaagatt attttactgt
     9301 tgctgtgctt gtaatcaatt caacagcttt tttgattgtg atatcatgtt tcaacatgtc
     9361 agctgaaagc aagctttgta cttgagcaac ttccatgttg tagtctgctg ccaattgctc
     9421 aacttctttt tggatttctt cttctgaagc atcaaatcct tcagctttgg caactgcttc
     9481 gataacaagg ttagtcttag tacgtgactc agcttctgct tggtattggt tgtgaaggtc
     9541 ttcttgagta gttccagtga tttggaagta catgtcaggg ttgatccctt gacgttgcaa
     9601 gttcccaagg aattcattta ctgaacggtg aacttcttca tggatcattt cttctggaag
     9661 ttctacgatt tcagcgtttt ctacagctgt atcaattgct gcaccttcaa ctgcatcttt
     9721 gtaagcttct tctttagcag cagccaattc tttgcggtat ttttctttca agtcagcaag
     9781 tgtttcaact tcttcatcaa tgtcttttgc aagttcatcg tcaagagctg gaacttcttt
     9841 agcttttact tcgtggatag ttgtcacgaa tttagcttct ttacctgcaa ggtcttctgc
     9901 ttggtagtct tctgggaatg ttacgataac atcaacggtt tcgccagctg agtgacctac
     9961 caattggtct tcgaaaccag ggatgaattg acctgaacca agtccaagtg agaagttttc
    10021 accttttcca ccgtcaaatt caacaccgtc gatagaacca acgaagtcga tcacaacagt
    10081 gtcgccgttt tcagcagcag cttccttgat aaccaattca gccaggttgt tgcgttcgcg
    10141 ttcgatacgc tcttcaacat cagcgtcagt tacttctttt tctacatcaa ctgatacttc
    10201 aaggtttttg tagtcaccca attttacttc aggttttgta acgacttcag cagcgataac
    10261 ccagtcttga cctttttcca ttgaagttac gtcaattttt ggttgggcaa ccacttcaag
    10321 accagcttct tttacagctg cttcataagc gtttggcaaa agagcgttca ttgcatcttg
    10381 ataaagagct tcttcaccaa atttttggtc gaagataggg cgtggaaggt gacctttacg
    10441 gaaacctgga acattaagag atttcttcac tgacttgaag acacggtcca attctggttt
    10501 gatttggtct tgagagatag tgaaagtcaa gacaccacgg tttgtttctt tgttttcaaa
    10561 tgatacagac attctgtcat ttctccttaa aattttttaa atacagtcta ttataacatt
    10621 ttctataaat aaaatctaga ctaatgcagc caattataga caacaagcct ttttcctaac
    10681 taaaacactc ataaagtata gacaatttta gac
//
LOCUS       DAVKGC010000066        10570 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKGC010000066 DAVKGC010000000
VERSION     DAVKGC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.066"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(78..1502)
                     /locus_tag="VW049_001415"
     CDS             complement(78..1502)
                     /locus_tag="VW049_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9432514.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHTAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2047..4326)
                     /gene="pulA"
                     /locus_tag="VW049_001416"
     CDS             complement(2047..4326)
                     /gene="pulA"
                     /locus_tag="VW049_001416"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9432515.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAAFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4438..6396)
                     /gene="ligA"
                     /locus_tag="VW049_001417"
     CDS             complement(4438..6396)
                     /gene="ligA"
                     /locus_tag="VW049_001417"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9432516.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6489..7667)
                     /locus_tag="VW049_001418"
     CDS             complement(6489..7667)
                     /locus_tag="VW049_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9432517.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7747..8610)
                     /locus_tag="VW049_001419"
     CDS             complement(7747..8610)
                     /locus_tag="VW049_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9432518.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8613..10514)
                     /locus_tag="VW049_001420"
     CDS             complement(8613..10514)
                     /locus_tag="VW049_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9432519.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3172 a         2336 c         1892 g         3170 t
ORIGIN      
        1 ttcgtatttt ttctataatt attattattt tatagcaaaa aaataaaacc aggaaaacaa
       61 tttcctggtt ttacacgtta tttcacatca aaaacaatgg atttgacatt tgtcatcgct
      121 tcaatgctat atttaattcc ttgcactcca gctccagaac ctttgacacc aaggaatggg
      181 aaattatctg gaccacgctg ggttttatta ttaatgtgga ctgtacctac ttcaagtttt
      241 tcagcaattt caaaggcttt tttgaaatca tttgtaaaga ctgatgattg aaggccgaat
      301 tcagattcgt tggcaaaggc aatagcttcc tctacactag ccacacgaat gattggtaaa
      361 acaggaccaa atggctcttc ccatgccact ttcatatctt ttgtaacttg gtcaaaaagc
      421 actggccaga ggagattgcc ctcacgtttg attggtgtaa gagcctgagc ctctttttct
      481 tgtgcatcct caatcaagcc ccaaatgaag tcggctgaag cattgtcaat aacaggtgta
      541 atatcagcat tgtcaaatgg atcaccaact gttaatttag aaacttcttc ctgaagcaaa
      601 gtagctaatt tatctgctac actttcgaga acaatgacac gtttaatggc cgtgcaacgt
      661 tgtcctgagt agctaaaggc tcccgcaaca atttgcttgg cagtatgttc caaatctgca
      721 tcttctagta caagagctgc atctttccca ccaagttcca acatgatagg acgcatacca
      781 gctaaacgac caatacgttc tccaatagga gttgaacctg taaagttgat gaaattgact
      841 tctttgtgct caatgatata atccccaatt tctgaaccac gacctgtaat ggtgttgaaa
      901 acacctgccg gaatccctgc ttcttcaaat gctttagcca acaagagtcc agaaatggaa
      961 ccttgtgttg gtggcttaaa catgaccaca ttccctgcaa tcaaggcagg tgcaatttta
     1021 gaagcagata aattaactgg ataattaaaa ggagcaatcg ctagcacgat accaactggt
     1081 tcacgacgga caacagccag tttgttttta cttgctgcct caaaaccacc accttccatt
     1141 gcttgtccag tgatacggag accttcctca gcagcataac gaatcaagtc tgctgtacgc
     1201 actacttctc caattgctgc tttaatccct tttgctactt ctttggcaag gatagtacca
     1261 attttttcct tatcgcgttc taaaatagct gctgttttat gcaaataagc cgcacgttca
     1321 attgctgata aagctcgcca tgctggcagg gctgcacgcg cagcttgcat agcctcatca
     1381 gcttcagtct gagtcatggc tggaactgta cccaattctt cttgattgat tggtgaataa
     1441 atcgtaattt cttgttcaga tgatttccat tttccattta ctaaattctg atatcttgtc
     1501 aaattcttgt cctcctgaaa atgattaaga tatattttac caaattttca gaaaatcaca
     1561 accctttttg caaaattttt gagattattt tcacaatctt gatttttcaa agtatactca
     1621 ataaaaatta aaaaaatcca ctacgtcaag gcgaggctaa tgtggtttga agaaattttc
     1681 gaagagcgtg aatgagtatc atctatagta aaataaaaaa actgaacaat ttggttgggg
     1741 acagccaaac caatttctca caatgtttca gaaacaaggg tgtgctattc caatttcagc
     1801 ctactataac tgtcatagat tgctgaaaca aagtctaggt aaaagtcttc ataataaaaa
     1861 gacctcctat caagtgttca aaaactttga taggaggtct tgttttgtga aaatatttat
     1921 caaattttct atacaagtga gctgttagcc aggttctttc tattctttca atttcaatga
     1981 atggattttt tactaatact cataactggg aatttgtctg tgtaaaaata gcgagataga
     2041 tggtatttat aaaacactca agacagctag actaatatca tttaaaacat tatcttcttt
     2101 tgagcgactg ttggttacca acatagctaa atttcctgca ttttcaaatt gatagggttc
     2161 tgatttagca ttcacaacca ccaagaggtg ttctttgccg tgaacttcat agataaggta
     2221 gccgctatgt tcaatcgcag aatgcacaaa gacatgatgg taaatttcat catagctaga
     2281 gtaagaaaag gcaccagttt ttgtcttcaa tcggatgact tgacggataa actcaatact
     2341 gtcttgacgc tcattaatca agttccagtt cacttggttc acactgtcag gagcattata
     2401 gctattcatc gcacgctctc tatcatcatg ggtcaactca ccattttcac cagtcgcaac
     2461 cagtttggta cgaccaaatt cttgaccgat ttccataaag gccatcccct gcatgagcag
     2521 attcatggct gtggcagttt cgaccttgcg catgatttgc tctgaacttt ggtctggatg
     2581 aagggttgcc aataaatcgt gaagattgta attgtcatgg gcttctacat agttaagcac
     2641 ctgatttgga tgtgtatagc ttcctaattc acgacttcct aggattgctt tagctagaat
     2701 tggctctgtc gcagcaccac tgacaaaacc tgacttgata gcaccataaa cttctccccc
     2761 tttgacagca tcgcgctgat tgtcattaaa gaaaccaata tttggcatct ggtaggcatt
     2821 gtccttcttg gccttatcat aaggggcaag acctgttccc atatcccatc cttctccata
     2881 gaggataatg ttggagtcga tttcatccaa gctttgacga atcatctgca tggtcttgac
     2941 atcatgaatc cccatcaagt caaaacggaa gccgtcaata ttatattcct gcacccagta
     3001 tagaagagaa tcaatcatat acttgcgaaa catttcgtgt tcactggctg tttcatttcc
     3061 aacacccgtt ccattctgga aggtaccatc tggattcata cgataatagt aatcagggac
     3121 tgttgtttgg aatgctgcat caacaactga gaaggtatgg ttatagacta catccataat
     3181 gactccaata cccgcatcgt gataagcttg aaccatcacc ttcaaatcac gaatgacctg
     3241 agctggatca tctggattag ttgaaaaact agtttctggc gcgttatagt tttgtggatc
     3301 ataaccccag ttgtaggtta catttccatc ctcatcgtat tctttatgac ggtctgcaat
     3361 tggttgcaat tgaacataat tgtagcccag cttcttgatg taatcaaaag cagttgactg
     3421 gccgtattgg ttaactgttc cagcctgagc agcacccaag aaagttcctc gaagatgttc
     3481 atctacaccc gatgtaggtg atttagtcaa atcacgaata tgcatttcac agataactgc
     3541 cttacatgga ttttccaagc gccaagtagc ctccgaaccg tgcttaacct cgaagttttc
     3601 aacttgcttt tctacatggc tcagaatagc tgaacgtttg ccatcagggc tggtcgcgat
     3661 tgtataagga tcacgtgtca gtgtttggtg atgagggaat tggacttgat actgataagt
     3721 cttacctacc aaatcttctt caacatccaa actccagaca ccgattgtat tgtccttatg
     3781 attataagag tagctattgc ctcttttcat ctcaaaagtc ttccaaacgg gtgcatcatt
     3841 agcagctgat tcataaacga caacttgcac ttctgtcgct gtaggtgacc agagggaaaa
     3901 atgagcctga ttgtcctcta cacggcaacc caattctcct tggtaacccc aatgatgatc
     3961 aaaactagca ctgttaatgg ccttatcaaa ggcaaaagga ttttgatttt tatagaaagg
     4021 actggcaata gcaggatttt cagagtaata aatcctatca tcgccttcca aaatccagac
     4081 ctctgttaat aggggatagt gattaaaacg gatagaatat tctttactag tttgacctgt
     4141 atgaaccaca aaattcaagc tttctataac atgtgaactt gggtgttcaa agctaaataa
     4201 agctccaaaa taatcttctt tgtaggttag caaatcaatt cgttgatcct gactttttac
     4261 aaaggagcaa gtgtcatatt ctccattctt acgatggtaa tgaatgcgca tagggtagtt
     4321 atacattttt tatttttcct ttttactttg tttctatttc actaataaat ttttgtcaat
     4381 ctcgtctcaa ttaacagaca tagtcatatt ctctaaactc tgtttttaaa cgatccatta
     4441 caaactttct agccatgcct catctctgac ctggatacca agttcttgtg ctttttgcag
     4501 tttacttcca gcgtctgcac ctaccacgac gaggtcggtc tttttagaaa cactacctgt
     4561 cactttggca cccagacttt cgagtttact tttagcttct gagcgcttga gtcgttccaa
     4621 ttttcctgtc aataccacgg tcaaacctga caaggccgca tccgctacta ccgtctgtcc
     4681 tttatagtcc agattgaccc cagtttcttt caattctctg agcagaattt cagagccttc
     4741 tgtcgcaaaa taagtctgaa gacttttggc aatcacgcca cctagacttt caatactagc
     4801 cacttcctct gaatctgcct gagacagatt ttcaattgaa tggaaatatt gaagtaaaag
     4861 ctgactaacc ttgcttccga catgacgaat tcccaaacca aataagagct tctcggcaga
     4921 attttccttt gatgcttgga tagcctgata cagtttagca gcggactttt ccttaacccc
     4981 ctctaaaagg aggaaatcct cttcttgcaa acgataaata tccgccacat ccttgactaa
     5041 attagcagca aaaagcttct caacaataga tggaccaagg cctgtaatat tcatagcatc
     5101 acgagaagca aagtgaatca agccttccat gatttgagca gggcaacgcg gattgataca
     5161 acgtagggcc acttcatctt caaagtgcaa caagtcagag ttacaacttg gacagtttgt
     5221 agggatatct agtttttctt cagaaacccg tttggactct accacacgta aaacggcagg
     5281 gatgatgtca ccagccttat atacaatgac cgtatcgtct tttcggatat ctttttcagc
     5341 aatataatct acattgtgca gggtcgcacg gctaacagtc gtaccggcaa gttgtactgg
     5401 tgttagatta gcagttggag ttacaacacc ggtacggcca actgtccagt caactgataa
     5461 gagttgagct tctttttctt cggcagggaa cttgtaggct actgcccact ttggagcctt
     5521 aactgtaaaa ccaagttctt cttgacttgc taggtcgttg accttgatta ccactccatc
     5581 aatatcgtaa ggcagatttt cccgttcctg tcctacttct tggataaaat tccagatttc
     5641 atctatgttt tcagccaaga ttcgcttagg attgaccaca aaacctagtt gttctaggta
     5701 cttcaaaccc ttttcttggc tatcacgagt tgaagggctg gcttcttgat agagaaacgt
     5761 tgcaagatta cgcttggcaa ctactgctgt atccaactga cgcagagttc ctgctgccgc
     5821 attacgagga ttagcaaatt caggctctcc attttcttgg cgcacttggt taacttggtc
     5881 aaaggaagcg cgtggcatgt aacattcccc acgaactgtg atatctagtt cttctggcaa
     5941 agtcaaaggg atgtccttaa cacgcttgag gttttctgtg atattttcac caattgaacc
     6001 atctccacgt gttaccccag caaccaaaat ccccttttca taagtcagcg agatagataa
     6061 gccatcgatt ttcagctcac aaatataggt cggatgagcc acttccttac gaacacgcgc
     6121 atcaaaagca tctagctcct cacgtgaaaa agcatcctgc aaactataaa gaggatactg
     6181 atgactgtat ttttcaaaac catctaaaac cttgccacca acacgatgag tcggactgtc
     6241 tgctagcact tgctctggat aagcagtttc taactcgacc aactcacggt aaaggcggtc
     6301 atactcactg tctgcaaccg agggattatc gctggtatag tactcagtcg catagcgatt
     6361 gagcaaagcg actaactcat tcattctttt attcataaga ccattttacc ataaaacaag
     6421 ccctcctcac aaacgagaag ggcggaaaaa acacttagtt tgaaattatt tttgaaactc
     6481 aagcaacctt atatcaattt ttcaaaatga gttcgaacat aaataaacga tatacaagac
     6541 aagatgataa caccacttcc aattatcagg aaagaagaga gatgtacact tggcaagact
     6601 gtcataaatc cttttgcaat aggcataaat agaatagcta aggtaaaaat tgtactcagt
     6661 actcttccaa gaaattcgct ctcaaccttg gtttgtactt gagtaaaaaa gtgaatatta
     6721 aaaatcgtca taaacaattc acaaactaaa tttccagaaa aggaaagaaa agttggaagt
     6781 ggtaatccca tcataaaaac tccgacacct gtcaaagcca gtaaaatcaa aagattataa
     6841 atattagctt taattttact agctagaaga gccccaatga tggaaccaat agcccccata
     6901 gttaaaatac ttgcataggc tccttctgac ccgtaaagct gattcgaaaa gggaagtaga
     6961 aattcaaaag ctgcaaaaaa gaaattaacg ctggaagcta ccagcaaaag gaagaaaatt
     7021 tcttgctgat gccagatata gtgtaaccca tccttgatat ctacaaaaat atctctccca
     7081 gtaaaagcct ttttctcttg aacttttgct tcctcttttg gaaggaaagc cactagaaca
     7141 aaagcaatga aaaaagtcag cgagtctagc agtagcgtca tattgagact tgcaaactgt
     7201 aaaacaagga aggaaagaac aggagagcta acacctacaa cctgcaaaac cagctctaag
     7261 cgagaattat agatcacaat ctcatctttc tccaccactt cagttatgat agctttattg
     7321 gctgtgcgag aaaaggcaaa agcaatagcc tgcacaatgt tagcaacaat caaagcgcca
     7381 atcatccagc tatcattcct tatgaaagaa atagccagac aaagaatccc acaaacaaga
     7441 tctgccgtca ttaaaatctt acgacgagaa aaacggtctg aaataactcc gccaaaggga
     7501 ttgacgagaa tagatgtgac gagctcagaa atctgataca ttcctaaaac tgtctgtcct
     7561 atagtcccca tagaagccaa ccagacacta tttccataat catagagcat atttcccatt
     7621 ttattgatag ccccacggct aatcaactgc actgcatagc gattcatatt aaagctcctc
     7681 tcaaattttg aaactattgt atcaaaaccg aaaggagctt tttatttttt cccttatttg
     7741 ggaaaattaa cttttgacaa atttttcgta gtgttcctga taataggcta cttgctctgg
     7801 aagacctaac acatcaaaaa tatgcatggc ctcttgcatc tgcttacagc cttctttaca
     7861 ctgtcctttt tgatataagg caaaaccttt taaataatgg aaaacattac gctcataaag
     7921 cttaatacct ttgtcaataa tcttctctgt ataagcctca aaatagttgg cattataaaa
     7981 agaagaatgc tctaaacaat gctggtaaca attgagggcc aaaatcaaaa ctaatctctt
     8041 atggcgacta atctcttggt aaaattcctc cctctccata acttctctac caatccgagt
     8101 gacatagtct acatcgtaga aactatagag gttaccgaaa agaatcaact catacatggt
     8161 ccattcttct gttttgaaga gataatctgc taccttatcc aaatcatcct gcttcatatc
     8221 ataagtcgca tctctttgac aaatcagacc ttgtagcaaa atccagttca gctcaaaata
     8281 aagggaagtc gtcgaactct tagacttttc aagttgttct ctttgaagct tttgaaaacc
     8341 tgcaatatcg tttgaatagt aaagtgggat aatctgtgcc atcatagaca catgttcatg
     8401 attatgaaaa ttccttgcct tatccatgaa attttcgatt gttacatgaa tgttatccaa
     8461 aatctcaaag aaacgggaga ctgccaggtc agactcccca agctcaaagc gagataactg
     8521 agaggtagag caggattcgc ctgctgcttc ctttaaagaa taatttccac ttgttcgaaa
     8581 ttcacgaaat acttttccaa gatgttccat ctttacacct gatctgataa ttcttcccac
     8641 tcaagcatag cttcttcctg acgatggctg attttgtcca gctcagcctg taattccatg
     8701 agtttgtcgg catcgtttgt ttccaacatt tgttcagaaa tggcttggct ttgactttct
     8761 agctcttcaa tttcagcttc tagactttcg atttgtcgca tgagtttgcg aacttctttt
     8821 tgactttctt tctgggcctg atagtcattg actggacttg cttcctttgc ttgattgcta
     8881 gttgaagctt cctcagtctg actcatttct gctgttgctt tcttctcaac atagtagtcg
     8941 taatctccaa ggtagagagt tgaaccattc tcagacaatt ccaaaacatg agttgccaca
     9001 cgattgataa agtaacgatc atgactgaca aaaagcaagg taccatcaaa gtcaatcaag
     9061 gcattttcta gcacttcctt actatcaata tccaagtggt tggtcggctc atccagaatc
     9121 aaaaagttat tgttttccat agacaattta gctaaaagca aacgagcttt ttcgccacca
     9181 gatagcatgc cgactgattt tttaacatca tctcctgaga aaaggaaggc cccaagacgg
     9241 ttgcggattt caacttctgg tgtcagtttg aaatcattcc agagttcatc cagcaccgta
     9301 ttacttggtg tcagcttgct ttgggtttgg tcatagtaac caacctcaac attagcgcca
     9361 aagcgctttt cacctttgat aaaaggaatc tggtccacaa tagacttgat aaaggttgac
     9421 ttgccgatac catttggacc aacgatagcg acagcattca tcttacgaag atctaggttg
     9481 ataggttgtg acaaaacttc cccgtcataa ccaatagcag tattttcaac agtcaaaaca
     9541 acattgcccg acgttttttc agactggaag gtcatgttgg ctgctttctt gccagcttca
     9601 ggcttgtcca aacgctccat tttttctagt tgtttacggc gagattgagc acgtttggtc
     9661 gttgaagcac gaactagatt gcgattgaca aagtcttcca gagcagcgat ttccttctgt
     9721 tgcttttcat agttttttgc ctcagtagct agcttttgct ccttcaactc aacaaaacga
     9781 gagtaattcc ccacatagcg atccaaggaa tgcttggtca aatctagcgt aattgtcgca
     9841 accttgtcca agaaataacg gtcgtggctg acgataatga gggcaccgct atagtttacc
     9901 aagtaattct ctagccagga gatggtttca atatccaagt ggttagttgg ctcgtccaag
     9961 accaagagat tgggcttttc aaggagcatt ttagctagag ccaaacgagt attttgacca
    10021 ccagaaagct cagcaatttt catctgccac atagactcgt caaacttgaa tccattcaaa
    10081 atcgctcgaa tatcagcttc ataggtaaag ccacctgctt ggcgaaaatt ttcagataag
    10141 cggtcataat ctgacatcag tttatccaaa tcctcaccag acttttcacc catctccagc
    10201 tccatctgac gcagttgttt atctgtccga cgtaagtcat caaagacatg aagcatctca
    10261 tcatagatgg tattttcaga ctcaaaacgg ctatcttgag ctaggtaaga cagagaaata
    10321 tcttttttct tattgatttc tccactagtt ggctcctctt ctccaactaa aatcttcaaa
    10381 agagtagact tacctgcacc atttttccca acaagggcaa tccgatctcg ttcatcaacc
    10441 tgcaggttga tattatcgaa aagaacctct cctgcaaaag aacgttcaat tttattagct
    10501 tgtaaaataa tcatacaagt agtatagcat gtttccctaa ggcattcaag ataatcgtaa
    10561 gtcttttagt
//
LOCUS       DAVKGC010000067        10381 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKGC010000067 DAVKGC010000000
VERSION     DAVKGC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.067"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(385..777)
                     /gene="rpsI"
                     /locus_tag="VW049_001421"
     CDS             complement(385..777)
                     /gene="rpsI"
                     /locus_tag="VW049_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9432520.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(796..1242)
                     /gene="rplM"
                     /locus_tag="VW049_001422"
     CDS             complement(796..1242)
                     /gene="rplM"
                     /locus_tag="VW049_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9432521.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVVNAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1599..1916
                     /locus_tag="VW049_001423"
     CDS             1599..1916
                     /locus_tag="VW049_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9432522.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(1961..2773)
                     /gene="folB"
                     /locus_tag="VW049_001424"
     CDS             complement(1961..2773)
                     /gene="folB"
                     /locus_tag="VW049_001424"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEU9432523.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIEKMRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2816..3370)
                     /gene="folE"
                     /locus_tag="VW049_001425"
     CDS             complement(2816..3370)
                     /gene="folE"
                     /locus_tag="VW049_001425"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9432524.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3351..4673)
                     /locus_tag="VW049_001426"
     CDS             complement(3351..4673)
                     /locus_tag="VW049_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9432525.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFNSPYLIHYTDQISINGESISEARLEALMADY
                     QSLMGGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(4675..5649)
                     /gene="folP"
                     /locus_tag="VW049_001427"
     CDS             complement(4675..5649)
                     /gene="folP"
                     /locus_tag="VW049_001427"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9432526.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFNQTFTVEELADFEILPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRNLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5744..6451)
                     /locus_tag="VW049_001428"
     CDS             complement(5744..6451)
                     /locus_tag="VW049_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9432527.1"
                     /translation="MKEKNMWKELLNRAGWIMVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIIGARKTQLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(6504..7922)
                     /locus_tag="VW049_001429"
     CDS             complement(6504..7922)
                     /locus_tag="VW049_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9432528.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            8112..8924
                     /locus_tag="VW049_001430"
     CDS             8112..8924
                     /locus_tag="VW049_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9432529.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKSFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9263..10282
                     /gene="adhP"
                     /locus_tag="VW049_001431"
     CDS             9263..10282
                     /gene="adhP"
                     /locus_tag="VW049_001431"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9432530.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3070 a         2345 c         2000 g         2966 t
ORIGIN      
        1 cttccccatt ctatggaatc ttgcattatc cataatatag tagattgaaa ctagaatagt
       61 acacctctac ttctaaaaca ttgttagaat tcgatttgac tatcctcgat ttgtcctatt
      121 cttatttcac attactataa aaataaggta aacttttcaa aaaagcacga aaaaagcacc
      181 cataaggtaa acttttgagt gctttgaaac attgatataa tcgtattgat taacgttttg
      241 aggcaactga ccttgtcagg ttgcagccac atttgtaagc gactaaagtc gcaacaactg
      301 tgtcaattgc accaatttag taagaaagta taaaaaaaga acaccccgaa aggtgctctg
      361 tataagtata gtaattcttt cgaattaacg tttactaaat tgtgatgctt tacgagcttt
      421 cttaagacct ggtttcttac gttcaacttt acgtgagtca cgtgtaagaa gtcctgcgcg
      481 tttcaatgaa tcgcggaagt ctgggtctac ttgaagaagg gcacgagcga taccgtgacg
      541 gatagctcct gattgaccag cgtatccacc acctacaacg ttaacgaaaa cgtcgtatga
      601 acctacagtt gaagtaactg cgaatggttg gttgatgaca agacgaaggt cagcgtgtgg
      661 gatgtactct tcaacatctt ttttgttaac agtgatttta ccagttcctg gaacaaggcg
      721 aacgcgtgca acagcgtttt tacgacgtcc agtacctgca tattgtgctt gtgacatact
      781 ttattgttcc tttccttaga taagtcctga aatgtcaaga acttctggtt gttgtgcagc
      841 gtgagtgtgc tcagctccaa caaatacttt caacttcata ccttgagcgc gtccaagtgt
      901 attgtgtgga agcatacctt taactgattt ctcaatcaaa cgtactgcat ttttagaacg
      961 aagttcacct gcagagattt gtttcaatcc acctgggtgg tttgagtgag tgtagtagat
     1021 tttatcagtt gcttttttac cagtcaattt aactttttca gcattgacaa caatcacgaa
     1081 gtcacctgta tcagtgtgtg gtgtaaatgt tggtttgttt tttccgcgaa gtacgctagc
     1141 aactactgca gaaagacgtc caagtggtac atcagttgcg tcaactacgt accatttacg
     1201 ttcaacttgg cctggtttag ccataaatgt tgttttgttc atgatttctc ctatatatag
     1261 ttcgattttt gtttacggtg atgggtgttc cttcccatcg aaaagtattt ggaaggttcc
     1321 ggggcctttc aaatggggta aacaataccg cctactatca taccaaattt cacccctaaa
     1381 agtcaataga tatgaaaaat atttttcggt aacttctcaa agtaactgaa agttatataa
     1441 tcaagttaat ttgggaattt acctattaac ttcggtagat ttttgggaat ttttttcctt
     1501 ctttttcctt gtagttaaaa tccttttcaa aaaatcacct tttcttgatt tttaatccta
     1561 tttgctgtat gataagggaa aagaaagggg acagagatat ggcttttacc aatacccaca
     1621 tgcggtctgc tagttttggc attgttacca gcttgcccga tgacatcatt gactcttttt
     1681 ggtatatcat cgatcatttc ttaaaaaatg tctttgaatt ggaagaagaa ctcgagtttc
     1741 aattgcttaa taaccaagga aagattacct tccacttttc aagtcaacac ctccctacag
     1801 ccattgattt tgactttaac caccctttcg accctcgtta tcccccaaga gtactggttt
     1861 tagacatgga cggtagagaa actatcctcc tcccagaaga aaatgaccta ttttaaaaac
     1921 tctagccttc agttgcaagt aactgaaaac tagagttttt ctattttttc aaagcatcat
     1981 acaagttgcg gatcggttgt tttaatatcg gatggataaa atgaggcgca atttcctgta
     2041 aggactcaag gacaaaaagg cgttccgcta tgtaaggatg aggcaatatg aggtcgtctg
     2101 tataaaggat ctggtcctcc acaaagagca agtccaaatc aatcaaacga ggtccccaat
     2161 gcacttctct cacccgtccc agctctgact caatggctaa caaggtttct aacaagtctt
     2221 gtgctggtag ccaggtttcc acctcaacca cttgattggc aaagctatcc tgctccactc
     2281 caccccaagg ctccgtcgct aagacactgg actctttgag aatatagatg ccacgagctc
     2341 gcattttctc aatggcttgt ttcaagtttg cttgtttatc tcccatattg cttcctaggg
     2401 cgataaaggc tcgttgcttg cggcgatgaa tggttaccga gcaagtatct agtgacaaat
     2461 gcaccggtgc ccaaggtttt ttcagttcca acttcatttc ttggacaaga ggataagact
     2521 caaaggtacg ttccaccagt ttataggcta ccgtttcaat caaatcttca ctcgtttcct
     2581 gaaaccaagt cgtccactgc tgacacaatt ctccgtaatg gacagaggct gttaaatcca
     2641 agtctgtagc tgccttggtc atatcatagg ataggatggc tgaaacgaca aatttctgcc
     2701 ccaattcttt ctcactagga aaaagaccat gataggcaaa catttccaaa tccttaatct
     2761 gcagttgatc cataatgatt cctttctaga aaaatccgct taaagcggat tctttttata
     2821 gccccattaa acgataagct tgatcgcgga ggtccttatc tgtttcaaat agaccacgag
     2881 ctactgtcgt caaggttgca gtacctggtt ttctaacacc acgcatactc atacacatat
     2941 gttccgcctc aatgacaaca aaggctcctt tagcacctag atagtccatc aaggcatcgg
     3001 ccacttcgat attcaaacgt tcttgaattt gtggtttttt cgaataaact tcaaccgtac
     3061 gggctagctt agacaagcct gccacacgac catctggaat gtaggcaatg tgcgctctac
     3121 cataaaatgg caagaagtgg tgttcacaca tggtatggaa aaagatatcc ttttctacca
     3181 ccatattatc gtcaataatt tcaaaggatt ttgacaaatg ttcctctgct gtttgaccaa
     3241 gacctgaaaa aatctcttga tacatacggg ctacacgagc aggtgtttcc tgcaagccct
     3301 cgcgattagc gttctctcct acagcctcga taatcatttt tacagctgct tcaatctttt
     3361 gtgtatccat tctcgttctt cctctccatc agataggccc tcacttggct caagaaatac
     3421 aaggaacctg tgacaatcct aactgtttgt ttctcttctt ttttatctgt caaattctgc
     3481 tctagaaaat catgccaatc ttggtagctg agatttctag acttagctgc ctctttcagc
     3541 acgctttcat ccgtcgcccg actatccgca aaatgtgtta gagtaagctc ggtatctggc
     3601 atggctccca gcaagtccaa catatcctcc aaggctttgg ttttgataca agtgaagagg
     3661 atttccttat gataatccgc aaaacgttct tgcaaggtta ccaacaaggc cttgatagca
     3721 tggggattat gggctccatc caaaatcatc aagggatctc ttgacacgat ttccaaacgc
     3781 cctggccaac ttgtttcttc caaggcttga ccaagaaagt cattgcttgc tagctctcga
     3841 ccatcttctt gacaaaaagt atcaagtaaa gctatggcca tcccagcatt ctctatttgg
     3901 tacaaaccaa gcaggctagt ctggaagcga ccttgtctga cagcacttgt atagtcaaag
     3961 acttcccctg tcaccacact ttcttgatga cgaacctgat aatctgtccc gtaggcaagt
     4021 ctcggcgcat ctttcccttc cgcaatgcgg tcaatcacag ccaaggcttc tggagcaata
     4081 cgccctgtta ccaagggcat cccttgtttg ataatacctg ccttctgctc tgctatggcc
     4141 tccaaggtgt caccaagtag ggccacatga tccaagccaa tagttgtaat tcctgtcaaa
     4201 atgggctgac agacattggt actatccaaa agtccaccca tgccaacttc catgatggcc
     4261 acatctactt gctctgaggc aaagtagtca taggccaggg ctgtgataat ctcaaactcg
     4321 gttgtgccct gtaaattggc gaccgcttct ccccccatca aagactgata gtctgccatg
     4381 agagcttcta gcctcgcttc tgagatcgat tccccattga tgctaatctg gtctgtgtaa
     4441 tgaatgagat agggcgagtt aaacacgcca actctcagcc ctagcttttc tagcatcttt
     4501 ttcaaaaaag caatagtcga gcccttgccg ttagtccctc cgatatggag gaccttgagt
     4561 ttgagatggg gattgccacg caaagctaac agttccacca ttcgttccaa accaaaatgc
     4621 ggttgatccg tccggtagtt agcaatccac tgattgtttt caatttcttt catcttattt
     4681 atattgtttt aaatctaaat tttccgcttc atcagccaga cgaatggcag aggcaatttc
     4741 aactgccatc ctgtgactag ctacgtcatg cacgcgcacc acttctacac cctgtctcgc
     4801 agcgatacta gttacatgag ccgaagccgt gtcccgattt cggaaaccaa gctctgtctc
     4861 aggattgact tcaaaaccat tctcctctag gatattgatg acaaatcgct tgcgcgaaac
     4921 tccgagaaag attggatagc ctttctgatg tagtttatcc aggttccgta aaagaagcag
     4981 attttctttc ttggtcagac caaagccaat tcctggatcc aacaggatat tttctggtgc
     5041 aataccagct tctgccgctc tcgctagtgc tcgttcaaag aaagccacca tcaagtcttc
     5101 gattggcaat atttcaaagt cagctaactc ttctactgta aaagtttgat taaatccaaa
     5161 atgagggaat atgagcgagc tagggtgttg aggtcgcgcc ataactggat taaacatgat
     5221 gaccacttgc gctctcgctt cagctaccac atgagccatt ttttcatcgc ccatgagacc
     5281 agtgatatca ttgactagat cggcaccagc agccaaagca gcctctgcta cttgactctt
     5341 ccaagtatca atagagatga ggacatcact ttccttgcga atcgctttga tcactggaac
     5401 aacacgctgg atttcctctt ctatctcaac atagctactg cccggccgag tcgattctcc
     5461 gccgatatcg agcatactgg ctccttctgc tatcaattta cgagcctgct ggagtgcctg
     5521 ctcaagagca aaaaattgac caccgtccga aaaggagtct ggggttacat tgataattcc
     5581 gcaaataact gtctttgcat gattggcttt acttgacata tcggtcactc cctcaaggat
     5641 tttcatcata ttatttttct attttaccat aaaaagaaaa agatggacac gattgcattc
     5701 atctttccct agtagaaaga ggtcggttcc tacataaaaa cttttacaca gaaattccta
     5761 atgtccgact cataatcacc acaagagcca acaaacagaa agcaatccca ttaacaatca
     5821 tgtgaagcaa gatcgacatt tccaaacgtt gggtcttgta ggccgtccaa gatagaactg
     5881 tcgacatacc tccataaatc aataaagaag gtaaattact tggttgatgc aataaagcaa
     5941 acacaatcgt accgactaca aatcccaagt tctccttgcc tcggaaaatc tttttaggaa
     6001 caatcccacg gcacaagatt tcctcacaaa tcggagcaag caaggctagc aagaagaaac
     6061 tggaaatcaa cgaactattt tgaaccatat cgttaatctg agactggtta gctgttgtcg
     6121 tctcatttga cagttgcaac aagatagaac caagtatatt tgacccgaca ataactaaat
     6181 aactcaagcc caaacgtgcc aaatctttag ctctaaaaaa agaaaaatta aaacttgcca
     6241 attgcgtttt acgagctcca ataataaata gagccagaac cacaattgaa aggccagcaa
     6301 ctatcagccc tgactgtagc agggctactt cttttaaagt caaaatagag gtaaccacta
     6361 ggggaacctg atataaaagg acggcaagta aaaagaccat aatccagcct gcacgattca
     6421 acaattcttt ccacatattt ttctctttca tcacctatct cctttgtatt aaaaaagggg
     6481 ggaaatcccc ctgggtcatt ctattataag gccatgctga tgtagttaag gataaacaag
     6541 gcatccaaaa tccaaatcat ggcatgaaca tctttaactt gacctttaac aagcttagtc
     6601 aaagtgtaag tcaagaaacc aactgcaatc ccttgagtga tagagtagct gaatcccata
     6661 aagatagatg tgaagaaggc aggaactgct tcagacatat catcccaatg gatatttttc
     6721 aagctaccaa gcatcataat cccaacgata atcaagattg gagctgtagc cgctgttggt
     6781 acgatcgcta gaagtgggct aaagaagctt gaaatcgcaa aacagatagc tacaaccaag
     6841 gctgtcaaac cagtacgtcc acctgcaccg ataccagcag cagactcaac ataagtcgtt
     6901 acgtttgaag tacctgcaat agcaccgact gtcgttccaa tcaaatcaga gtaaagagcc
     6961 ttatccaatt tggctgattg gtgattttca ccatttgtcg ctacgatacc aactttttca
     7021 cctgtaccga tcaaggtacc aattgtgtca aaaatatctg tcaatgagaa ggcaagaatg
     7081 gccatcagag tttcaggcaa gcgagctgta tctgaaacca aagctcccaa accttctgaa
     7141 ccaagagctg caccaaagat tgtcttcaaa tcttcaaaag ctgcaccaac atggttatta
     7201 gcaaaatcga tactagacaa atcaaccaaa ccaactgcaa tagcaagaac agttgttgtc
     7261 aagatagaga gaataattcc ccctttaatc cctttgatga caaagaagat agtaatggca
     7321 agtcctgcaa gagccactaa aacagctgga ttattaaagc tgaccaatcc tggaactgct
     7381 gaagagtttg ctgcaatcgt tgcttgagct ttgtcagccc cttctcctac aacagtatag
     7441 ttgcctggat caatcgtgaa tttcaaaagt ccagcattct taatccctac ataggcaagg
     7501 aagacaccga taccagctga aatagctgag cgaagagcat tgggaatcga ttcaatgatc
     7561 attttacgaa catttgtcaa ggtaataatc aatgaaataa tcccacagat gaagaccata
     7621 gctagggctt cttgccaaga ataaccaagc ccgaatacaa ctgtaaaggt aaagaaggca
     7681 ttgagtccca tacctggcgc ttgggcataa ggtaagttag cataaaaagc catcatcaag
     7741 gtacccgcta ctgcaccaat aatcgtcgct aggaagacgc cctgagcagg cattcctgtt
     7801 tgtgaaagta tttgtgggtt tacaaagaga atatagctca ttgcaaagaa agttgttaaa
     7861 ccagcgagaa cctctgtacg aacgtctgta ccgttctctt ttagtttaaa taatttgtcc
     7921 atcatcaatc tccttttaat ttttacgaac aataatagaa ttatatcatt tatcattcac
     7981 tttttcaatg tctttgtttg atttgtttaa gaaattaatc caatttcatt ttttgtttgg
     8041 aatctgcttt tctgcctgct ttctgttata atagaagaca tcttatctga aaacacacta
     8101 gaaaggtcca tatgacgaaa aaaattattg cagttgacct ggatggaacc ctgctcaact
     8161 cagacagtca aatttctgac tttaccaaaa gaaccatcaa aaaagttgct gaaaaaggcc
     8221 atcaggttat tattacgaca ggtcgccctt accgtatgtc aaaagatttt taccgtgaac
     8281 tgggcttaga cactcctatg attaacttca acggatccct tactcattta ccagaccaag
     8341 tttgggattt tgaaaagtgt ttgactgtag acaaaaaata tctgctagat atggttcaac
     8401 gttcagagga cattcaagcc gattttatcg ctggagaata tcgtaaaaaa ttctacatta
     8461 caaatcccaa tgaagaaatt gccaatccca aactatttgg tgtagaagct ttccagcctg
     8521 aagatcaatt ccagcctgaa ttggtgacca aggaccctaa ctgtatcctc ttgcagacca
     8581 gagccagtga caaatatgcc ttggcaaaag aaatgaacgc cttctaccag catcaacttt
     8641 ctatcaatac ctggggaggt ccgctcaata tccttgaatg taccccaaaa ggtgtcaaca
     8701 agtcctttgc tttggactac ttgctcaaga taatgaatcg tgacaaaaaa gatttgattg
     8761 cctttggaga tgaacacaat gataccgaaa tgctcgcttt tgctgggaag ggttatgcca
     8821 tgaaaaatgc caatccagag ctactccctt atgcagatga gcaaatttcc cttaccaacg
     8881 accaagatgg ggttgccaaa accctacaag acttattctt ataacctata ctgatactca
     8941 atgagaatca aagagcaaac taggaagcta gccgtaggtt gctcaaagca ctgctttgag
     9001 gttgtagatg aagctgacgt ggtttgaaga gattttcgaa gagtatgacc agcttgcaag
     9061 ctggtctttg tgctcttttc acagtctcat caaccagtta atcgattgtt ctactttttg
     9121 tctccaaaac cttggaaaag gctttctttt gtgatatact tcacatataa atacaagaga
     9181 gctcgtcaca ctcgactctg ttgacaaaaa atcaatccag tcccgttgtc cctgttctcg
     9241 gtcaatatca aggaggatag ctatgaaagc tgttgttgta aatccagaaa gcactggtgt
     9301 tgctattgaa gaaaaagtac tccgtccact tgaaactggg gaagcacttg tagaagttga
     9361 atactgtggt gtttgccaca ccgacctcca cgttgctcat ggtgactttg gtcaagtccc
     9421 aggacgtgtt cttgggcacg aaggtatcgg tatcgttaaa gagattgctc cagatgtgaa
     9481 aagccttaaa gtcggtgacc gcgtcagcgt tgcttggttc tttgaaggat gtggcacttg
     9541 cgaatactgt acaactggtc gcgaaaccct ttgccgtaca gtgaaaaatg ctggctactc
     9601 agtagacggt ggtatggctg aacaatgtat cgtaactgct gactatgctg tcaaagttcc
     9661 tgacggactt gatccagccc aagcttcttc tatcacatgt gctggagtaa caacctataa
     9721 agctatcaaa gaagcaaaag ttgaaccagg ccaatgggtt gttctttacg gtgctggtgg
     9781 acttggtaac ctcgctgttc aatacgctaa aaaagtattc aatgctcatg ttatcgcagt
     9841 cgatatcaac aatgacaaac ttgcccttgc aaaagaagta ggcgctgaca ttgtgattaa
     9901 cggcctcgaa gttgaagatg tagctggact cattaaagaa aaaactgatg gaggagctca
     9961 ttcagctgtc gtaactgctg tgtctaaagt tgccttcaac caggctgttg actccattcg
    10021 tgctggtggt cgcgtcgtcg ctgttggtct tccttctgaa atgatggaac tcagcatcgt
    10081 taaaacagtc ctcgatggaa tccaagtcat cggttctctt gtcggaactc gtaaagactt
    10141 agaagaagcc ttccaatttg gtgcagaagg tctggtagtc ccagttgttc aaaaacgtcc
    10201 agtagaagat gctgttgcca ttttcgacga aatggaaaaa ggccaaatcc aaggacgtat
    10261 ggtactcgac ttcacccact aatctaatag aaacctattt taaaagttgg aagcttcttc
    10321 caactttttt atattaaatt ttaaataaga attcaagatt actcctcaaa aactcaacat
    10381 a
//
LOCUS       DAVKGC010000068        10308 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKGC010000068 DAVKGC010000000
VERSION     DAVKGC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.068"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..170
                     /locus_tag="VW049_001432"
     CDS             <1..170
                     /locus_tag="VW049_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9432531.1"
                     /translation="TVSFAISSKGEKMKVENISYRVDHRILFDNISFATSSSGVTLIT
                     GKNGTGKSTLL"
     gene            complement(521..1831)
                     /locus_tag="VW049_001433"
     CDS             complement(521..1831)
                     /locus_tag="VW049_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9432532.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2068..2940
                     /locus_tag="VW049_001434"
     CDS             2068..2940
                     /locus_tag="VW049_001434"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9432533.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3071..3900
                     /locus_tag="VW049_001435"
                     /pseudo
     CDS             3071..3900
                     /locus_tag="VW049_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3961..4923
                     /locus_tag="VW049_001436"
     CDS             3961..4923
                     /locus_tag="VW049_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9432534.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAEASKIAQ
                     FQTKIVCVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQKVLDIVIIFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVEPLRYE"
     gene            5100..6107
                     /gene="gap"
                     /locus_tag="VW049_001437"
     CDS             5100..6107
                     /gene="gap"
                     /locus_tag="VW049_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9432535.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(6174..7010)
                     /locus_tag="VW049_001438"
     CDS             complement(6174..7010)
                     /locus_tag="VW049_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9432536.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            7136..9331
                     /locus_tag="VW049_001439"
     CDS             7136..9331
                     /locus_tag="VW049_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9432537.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            9384..9536
                     /gene="rpmG"
                     /locus_tag="VW049_001440"
     CDS             9384..9536
                     /gene="rpmG"
                     /locus_tag="VW049_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9432538.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            9546..9722
                     /gene="secE"
                     /locus_tag="VW049_001441"
     CDS             9546..9722
                     /gene="secE"
                     /locus_tag="VW049_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9432539.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            9777..>10308
                     /gene="nusG"
                     /locus_tag="VW049_001442"
     CDS             9777..>10308
                     /gene="nusG"
                     /locus_tag="VW049_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9432540.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         3216 a         1779 c         2249 g         3064 t
ORIGIN      
        1 gaactgtttc ttttgctatt agttcaaaag gagaaaaaat gaaagtagaa aatatttcgt
       61 atagggtgga tcatcgtata ttgtttgata atatttcttt tgctacttcg agttcaggcg
      121 tgacattaat tactggtaaa aatggtacag gaaagtcaac tttactatag tagattgaaa
      181 ctagaatagt acacctctac ttctaaaatg ttgttagaaa tcgatttgac tgtcctgttc
      241 ttatttcatt ttactacatc tcaaattgag tatgacgaag tgcgctccca tgtcatggga
      301 acgcacttcg tcatattttt catattcttg aatccatcga taaagactat tgggatgaat
      361 ttttaaagtt gaactaatca tttttacagg atgaggttta cagcagagag tttgaaggct
      421 ttatcaaagg tttttcttgg cataatgact tttcctcgtt tccacttaat tttgtgtcta
      481 ctttattata ccaagtccac ccttaagtta gataataaat ctaacttaag gaagctagaa
      541 ggatgagaat ccaggtggtc aagagtccca aacttaagct gatggggaca cccagaataa
      601 tttgcttttt gaaggcaagg ccacgttcct ctatattggg aagtgagagt tgaatgagag
      661 aaccagctga tgaaaagggt gagatattag tagatagagc gccaataacg gtggctgttg
      721 tgagtaagtg aatatcaatc tgaggatttt gagcactgat gatagcaatg atgggaaaga
      781 gggctggagc tacaacggat agggtggaac taaagagtga catcactccg gctatcacac
      841 aaaagaacag aggtaaccag aaatgaggaa tggttgttgt catgaggtgc cctatcagtg
      901 tgactaaacc tgacttgacc gctagagaca ttagtaagct catgccgcag agcatgataa
      961 ttgtagccca gggaacctta gccaaaatgg cttcttgctt ccctaatttg agccttaagg
     1021 cgaggcagac catgagtatt gagacaaagc caatatcaaa tgtttttcga taagtagcta
     1081 tccaggcgat gtttgggaaa atgagatgca acaagggaaa aagccagacc aaaaccatgc
     1141 tgctgatcat gagcaaggtg gtttgtcttt gaaccttgct gaggagtggt ggttggtcaa
     1201 tagtcaagga tgagtttgtt cttcccttac tatagtggct gtaacaggat aataaaagca
     1261 agacgatgag tgggtagata atgctgacga taaagatatg attgccaagt gaaaaagctt
     1321 gctcttccca tcccatttgc ttaaacaggc cttgaaagac aatgcctgag ctactggtta
     1381 tcaaattagc ccctcctgaa gctccccaat tgacggcttg agctccaatc aaagggtgtt
     1441 tgtccgcttt ttgacagagg gtaatcgcta gaggacagca aacggccata gtagtgaaaa
     1501 atccagcacc taaagcagac aaaagggttg ccatcaggta taaaatcatg tagagggcgt
     1561 tagggtgggt gcgtgtgcgg tagagaatgt gttgagccaa aacatcaaga gtaccgttag
     1621 ttgttgcaac gttataaaag agagagacgc taaaaatggt aaaaaagagt gaggttggcc
     1681 aaaaatgaag aagttctttg gggcttaatc ccatgagagt ggttgcgatg aggtaagaaa
     1741 aagcaatagc cagcaggcca atattgattt tggtgcggta accaattcca atggctagag
     1801 caatggcgct aatcattatt aaatgaatca ttgaattgtc ctttcagtta gaatatggaa
     1861 atagattggc aattttggcg ctcaaaagac cacctgtttc ctattggtac ttgactatta
     1921 tacacaaaaa aaatacaatt gtcttgacaa ttgtattgac acttgttaga attaaaaatg
     1981 aggtattcag gcttgtgatt tcctatggaa gttaatagta attgcctcta gtgcttacaa
     2041 gtgatattaa aaatagagga cttagtgatg tcaatcattt caactgattt aacccctttt
     2101 caaatagatg atacattgaa agcagccttg cgagaagatg ttcattccga agattacagt
     2161 accaatgcca tttttgatca tcatggccaa gccaaggtgt cgctttttgc caaggaagct
     2221 ggtgttttag cgggactaac cgtttttcaa agggttttta ccctatttga tgccgaggtg
     2281 accttccaga atcctcatca atttaaggat ggggatcgtt tgactagtgg cgatttggtt
     2341 ttagaaatca taggctcggt gagaagtctc ttaacatgtg aacgcgttgc cttgaatttt
     2401 ttacaacatt tatcagggat tgcttcgatg acagctgctt atgtagaagc cttaggcgat
     2461 gattgcatta aggtatttga tactcgaaaa actactccta atttacgtct ttttgagaaa
     2521 tatgccgtga gagttggcgg tggctataat catcgcttta atttatcaga tgctatcatg
     2581 ctaaaagaca atcacattgc ggcagtaggt agtgttcaaa aggcaattgc tcaagcgcgt
     2641 gcctatgccc cttttgtgaa aatggtcgag gtggaagtgg aaagccttgc tgctgccgaa
     2701 gaagctgcgg cggcgggtgc tgatattatc atgttggata atatgtcatt ggaacagatt
     2761 gaacaggcta ttaccctaat tgcaggacgt tctcggattg aatgttctgg aaatattgat
     2821 atgaccacta ttagccgttt tcgtggttta gcgattgatt acgtctccag tggtagttta
     2881 acccatagtg ctaagagtct tgatttttcc atgaaggggt taacctacct tgatgtctaa
     2941 gttgtaaaat aaactaactt tttaaaggat gtttttcctc tagaacgagt tttatgtcag
     3001 atagtttaaa cgcctcttca aatatagtaa aatgaaccaa aaatagtaca caatgtggta
     3061 taatcttttt atggcatatt caatagattt tcgtaaaaaa gttctctctt attgtgagca
     3121 aacaggtagt gtaacagaag cataacacgt tttccaaatc tcacgtaata ccatttatgg
     3181 ctggttaaag ctaaaagaga aaacaggaga gctaaaccac caagtaaaag gaacaaaacc
     3241 aagaaaagtt gatagagata gacttaaaaa ctatcttact gacaatccag atgcttattt
     3301 gattgaaata gcttctgaat ttggctgtca tccaactacc atccactatg cgctcaaagc
     3361 tatgggctac actcgaaaaa aaagaaccac acctactatg aacaagagcc agaaaaagta
     3421 gccttatttc ttaagaattt taatagttta aagcacctag cacctgttta gattgatgaa
     3481 acaggattcg atacttattt ttatcgagaa tatggtcgct ctttaaaagg tcagttaaga
     3541 agaagatatt agaggatttc tttggttgca ggtctaacca atggtgagtt aatcgctcca
     3601 atgacttacg aagagacgat gacgagcgac ttttttgaag cttggtttca gaagtttttc
     3661 ttaccaacat taaacacacc atcggttatt attatggata atgtaagatt ccatagaatg
     3721 gggaagctag aatttttatg cgaggagttt gggcataaac ttttacctct tcctccctac
     3781 tcgcctgagt acaatcctat tgagaaaaca tgggctcata tcaaaaagca cctcaaaaag
     3841 gtattaccaa gttgcaatac cttttacgag gctcttttgt cttgttcttg tttcaattga
     3901 ctatatatca cggatacagt tgggaaagaa gctattaatt atttggagag tccttttggg
     3961 atgaacatgt caaatattac tatagatggg caagaaatgg atctgagtca aacgatgcct
     4021 tcttatgctc aagtagtgag tctttatgat gatacaagta tctctgttag taataatgag
     4081 acggacaaag tgttggctgg atccctctat actgatacaa atgaacaggg attaacgatt
     4141 ccaagcagtt tactaaaaaa ttggaatgaa cagacaggaa aaaatttgac agctaatgat
     4201 cttattggca aatcagtctc agccagcatt gtagaaagtg ctgccgaagc tagtaagatt
     4261 gctcaatttc aaacgaagat tgtatgtgta atcaatgatg aagatgacat ggaggacagc
     4321 aacagtttca tgctgtctca tcaaatggaa acgattttga aagaggctgg atttacgaaa
     4381 gctgtatctt attttatctt ggaactcaaa gatccatcac agacaaaagt agtaacagaa
     4441 gaattacaga aaaataagaa gtatactgtg ctttctcaac agaaagttct tgatattgtg
     4501 attatcttta ttcgtgttat tcagggatta ttgattgtgc tttcatcaca agctattgtg
     4561 gtagcagcgg ttatgattgg tatcattatt tacatcaata tcatgcagcg ttccaaggaa
     4621 ataggtgtca tgaaggcagt tggttatcag aatcgtgatg tcaaaggaat ttttatttac
     4681 gaggctatct ggattgtagg catcgccttg ctgctggcat ttttggttgc acaaggggtg
     4741 ggaagtttgg cgaatgcgat tgtaagtcac ttttacccat ccatcactaa agtttttgaa
     4801 ttaaatcttt tatctgtttt aggaactcta gttttcgctc tattacttgg ttatgtctca
     4861 gcctacttcc cggcgcgtaa gattagtaaa atggatcctg tagaaccgtt acgctatgaa
     4921 tagtaaggga ttccttgatt tacagtctta aaaagaaact aacatagaaa aacaatggcc
     4981 atacttgcaa ttcaactcaa atagttgtat aataggtttg ttgaaaggtg atttgtagtg
     5041 attcaagtta ctcttttcac aataaaaatt tcatgatttt cataaggagg aaatcactaa
     5101 tggtagttaa agttggtatt aacggtttcg gacgtatcgg tcgtcttgct ttccgtcgta
     5161 tccaaaacgt agaaggtgtt gaagttacac gcatcaacga ccttacagat ccagttatgc
     5221 ttgcacactt gttgaaatac gacacaactc aaggtcgttt cgacggtact gttgaagtta
     5281 aagaaggtgg atttgaagtt aacggtaaat tcatcaaagt ttctgctgaa cgtgatccag
     5341 aacaaatcga ctgggctact gacggtgtag aaatcgttct tgaagctact ggtttctttg
     5401 ctaagaaaga agcagctgaa aaacacctta aaggtggagc taaaaaagtt gttatcactg
     5461 ctcctggtgg aaacgacgtt aaaacagttg tattcaacac taaccacgac gttcttgacg
     5521 gtactgaaac agttatctca ggtgcttcat gtactacaaa ctgcttggct ccaatggcta
     5581 aagctcttca agacaacttt ggtgttgttg aaggattgat gactactatc cacgcttaca
     5641 ctggtgacca aatgatcctt gacggaccac accgtggtgg tgaccttcgc cgtgctcgcg
     5701 ctggtgctgc aaacatcgtt cctaactcaa ctggtgctgc aaaagctatc ggtcttgtaa
     5761 tcccagaatt gaatggtaaa cttgatggat ctgcacaacg cgttccaact ccaactggat
     5821 cagttactga attggtagca gttcttgaaa agaacgttac tgttgatgaa gtgaacgcag
     5881 ctatgaaagc agcttcaaac gaatcatacg gttacacaga agatccaatc gtatcttcag
     5941 atatcgtagg tatgtcttac ggttcattgt ttgacgcaac tcaaactaaa gttcttgacg
     6001 ttgacggtaa acaattggtt aaagttgtat catggtacga caacgaaatg tcatacactg
     6061 cacaacttgt tcgtactctt gaatacttcg caaaaattgc taaataattc ttgagttgat
     6121 agaaagcaag gctttgtggt cttgcttttt atatggaaaa atggatgaca cgatcatcca
     6181 ttctttttta attctttttc aaatgtattt gaaagggtag tgaaagttag cttctctaaa
     6241 gtaagtgggt gggtaaagga aagtcggaag gcatgaagca taagccgact tgtctttgat
     6301 ttactattat agagagggtc gcccaggata ggaagattat gatgcgaaag gtgcacacga
     6361 atctgatggg ttcgccctgt ctttagcttg caacgagcca aggaagtctt gtttgagaat
     6421 tgctttaatc tgcttacatg cgtttcagca tattgcccat tttttgcatc aactattctt
     6481 tttctacgat catggcgatc acgtccaatt ttgtctctga aaacaagttc ttttctgttg
     6541 atatttccat caactagagc ccaatattct ctagaaatct cttttttctc caataagcga
     6601 ttgagaatgg gcaggataaa aggatttttg gcaaagagaa ctaagccact ggtttccatg
     6661 tccagacgat gaacgacata gcaggtttgg ccaacatagg tactgacatg gttaagaagg
     6721 gcaatttcgt ttggttgatt accatgcgtt ttcatcccct ctggtttgtt tacaataatc
     6781 aagtgttgat cttgataaac ttcctgcact aagtctgggt tgccccaagg gatcgtcttt
     6841 tgggaataat cttcctcgtc aaaagtcaac tggcaaacat ctccaggatt tacgatttcg
     6901 ttccagtgga cttcttcttg atttatcaaa atatgtttct tgattctcaa aaaatgacgg
     6961 atttttctag ggatgaggag ttgttcctca agtaattgct ttactgtcat ttgaggtaga
     7021 gaggcgggta atgtaaatgt gaattgcata cagatattgt aacaaaaaaa gccctatttg
     7081 gataggaaat agctaaattc ttgtcttcct atgatgaaga tgataaaata aacgcatgaa
     7141 attagataaa ttatttgaga aatttctttc tctttttaaa aaagaaacaa gtgaactaga
     7201 ggactctgat tctactatct tacgtcgctc tcgtagtgat cgaaaaaaat tagcccaagt
     7261 aggtccgatt cgaaaattct ggcgtcgtta tcatctaaca aagattatcc ttatactagg
     7321 tttgagtgca ggcttgctag ttggaatcta tttgtttgct gtagccaagt cgaccaatgt
     7381 caatgatttg caaaatgcct tgaaaactcg gactcttatt tttgaccgtg aagaaaaaga
     7441 ggctggtgcc ttgtctggtc aaaagggaac ctatgttgag ctgactgaca tcagtaaaaa
     7501 cttgcagaat gctgttattg cgacagaaga ccgttctttc tataaaaatg acgggattaa
     7561 ctatggccgt tttttcttgg ctattgtcac tgctggacgt tcaggtggtg gctctaccat
     7621 tacccaacag ctggctaaaa acgcctattt atcgcaggat caaactgttg agagaaaagc
     7681 gaaagaattt ttccttgcct tagaattaag caaaaaatat agtaaggagc aaattctaac
     7741 catgtacctt aacaacgctt attttggaaa tggtgtgtgg ggtgtagaag atgcgagtaa
     7801 gaaatacttt ggagtttctg catcagaagt gagtctggat caagctgcga ctctggcagg
     7861 gatgctcaag gggccggaac tgtataatcc cttgaattcc gtagaagatt ctactaatcg
     7921 gcgcgatact gtcttgcaga atatggttgc agcaggatat attgataaaa accaagaaac
     7981 cgaagctgct gaagttgata tgacttcgca attgcacgat aagtatgaag gaaaaatctc
     8041 agattaccgt tacccctctt attttgatgc ggtggttaat gaagctgttt ccaagtataa
     8101 tctaacagag gaagagattg tcaataatgg ctaccgcatt tacacagagc tggaccaaaa
     8161 ctaccaagca aatatgcaga ttgtttatga aaacacatcg ctatttccga gggcagagga
     8221 tggaacgttt gctcaatcag gaagtgtagc tctcgaaccg aaaacagggg gagttcgtgg
     8281 agttgtcggt caagttgctg acaatgataa aactggattc cggaatttca actatgcaac
     8341 ccaatcaaag cgtagtcctg gttctacaat taagccttta gttgtttata cgccagcagt
     8401 tgaagcaggc tgggctttga ataagcagtt ggataaccat accatgcagt atgacagcta
     8461 taaggttgat aactatgcag ggatcaaaac gagtcgagaa gttcctatgt atcaagcctt
     8521 ggcagaatcg cttaatctac ctgctgttgc cactgttaat gatttgggtg tcgacaaggc
     8581 ttttgaggca ggcgaaaaat tcggactcaa catggaaaag gtcgaccgtg ttcttggtgt
     8641 cgccttggga agcggtgttg aaaccaaccc tcttcaaatg gctcaagcat acgctgcctt
     8701 tgcaaatgaa ggtttaatgc ctgaagctca ttttattagt agaattgaaa atgctagtgg
     8761 acaagttatt gcgagtcata aaaattcaca aaaacgggtg attgataagt ctgtagctga
     8821 caagatgacc agtatgatgt tggggacttt caccaacggt accggtatta gttcatcgcc
     8881 tgcagactat attatggcag ggaaaactgg aacaactgaa gcagttttta atccggagta
     8941 cacaagtgac cagtgggtaa ttggttatac tccggatgta gtgattagcc actggcttgg
     9001 ctttcctacc actgatgaaa atcactatct agctggctct acttcaaacg gtgcagctca
     9061 tgtctttaga aacattgcca ataccatttt accttatacg ccaggaagta cctttacggt
     9121 tgaaaatgct tataagcaaa atggaattgc accagccaat acaaaaagac aagtacaaac
     9181 caatgataat agccagacag atgataattt gtctgatatt cgagggcgtg cgcaaagtct
     9241 agtagatgaa gctagccggg ctatctcaga tgcgaagatt aaggaaaagg ctcaaacaat
     9301 atgggattcg atagtcaatc tatttcgcta agatgcttgt caaagcctag ctttcttgtt
     9361 ataatggata agatggaggc gttatggcac taaaaaaagc aagcctagct tgtgcggttt
     9421 gtggttcgag aaactattca atcaagatca gcggaaaccc caagcctaca cgactagaag
     9481 taaataaatt ttgtaagcat tgtggcaagt acactacaca cagagaaacg agataggaga
     9541 gagcgatgcg ttttattgga gatattttta gacttcttaa agacacaacg tggccaactc
     9601 gcaaggaaag ctggagagat tttcgttcta tcatggaata cacagctttc tttgtagtaa
     9661 ttatttacat ttttgaccag ttgattgttt caggtttgat tcgatttatt aacatttttt
     9721 agaagattag tggagttaat tacactagaa atcttctatt tatgaaagga aatatcatgg
     9781 atagttttga taaaggatgg tttgttttac aaacttattc tggttatgaa aataaggtga
     9841 aagaaaatct attacaacgt gcacaaacct acaatatgtt ggataatatt ctacgcgttg
     9901 aaattccaac acaaacagtg caagttgaaa aaaatggaaa gagaaaagaa gtagaagaaa
     9961 atcgctttcc aggttatgtt cttgtagaaa tggtcatgac agatgaagct tggtttgttg
    10021 ttcgaaacac accaaatgtt acaggatttg tcggatcaca cgggaacaga tcaaaaccaa
    10081 ctccattatt ggaacaagaa attcgtgaca ttttggtatc tatgggacaa actgttcaag
    10141 aatttgattt cgatgttgag attggtcaaa ctgtacgtat tattgatggt gcttttgcag
    10201 actacactgg taagattaca gaaattgata ataacaaagt gaaaatgatt atctctatgt
    10261 ttggtaatga cacagttgca gaagtaaacc taaaccaaat cgcagaat
//
LOCUS       DAVKGC010000069        10212 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKGC010000069 DAVKGC010000000
VERSION     DAVKGC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.069"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            85..1260
                     /gene="mutY"
                     /locus_tag="VW049_001443"
     CDS             85..1260
                     /gene="mutY"
                     /locus_tag="VW049_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9432541.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1316..2020
                     /locus_tag="VW049_001444"
     CDS             1316..2020
                     /locus_tag="VW049_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9432542.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2013..3362
                     /gene="vicK"
                     /locus_tag="VW049_001445"
     CDS             2013..3362
                     /gene="vicK"
                     /locus_tag="VW049_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9432543.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3364..4173
                     /locus_tag="VW049_001446"
     CDS             3364..4173
                     /locus_tag="VW049_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9432544.1"
                     /translation="MSKIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4264..4506
                     /locus_tag="VW049_001447"
     CDS             4264..4506
                     /locus_tag="VW049_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9432545.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4508..4762
                     /locus_tag="VW049_001448"
     CDS             4508..4762
                     /locus_tag="VW049_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9432546.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4809..5552
                     /locus_tag="VW049_001449"
     CDS             4809..5552
                     /locus_tag="VW049_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEU9432547.1"
                     /translation="MNKKWLFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     NSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILLEKQSEKIEELH"
     gene            complement(5853..6839)
                     /locus_tag="VW049_001450"
     CDS             complement(5853..6839)
                     /locus_tag="VW049_001450"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9432548.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7033..9501
                     /gene="gyrA"
                     /locus_tag="VW049_001451"
     CDS             7033..9501
                     /gene="gyrA"
                     /locus_tag="VW049_001451"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9432549.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLVITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            9501..>10212
                     /gene="srtA"
                     /locus_tag="VW049_001452"
     CDS             9501..>10212
                     /gene="srtA"
                     /locus_tag="VW049_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9432550.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILT"
BASE COUNT         3158 a         1737 c         2288 g         3029 t
ORIGIN      
        1 gatagaaaag ttatatacta tctattgaaa atattataaa tcagatacaa agacaggtga
       61 aaccttctgt gttatactag agatatgtta gatttgaaag aatacggtat cgtcatgtgg
      121 ccggaggaga aagtcatttc tttccgtgag aaacttctcg cttggtatga tgaaaacaaa
      181 agagatttgc cttggaggag aagtaaaaat ccttatcaca tctgggtatc tgaaatcatg
      241 cttcagcaga ccagggtgga tacagttatc ccttactacg aaagattttt ggactggttt
      301 ccaactgtcg aaagtctggc aactgcgcct gaggagagtt tactgaaagc ttgggagggc
      361 ttgggctatt attctcgagt tcgcaatatg caggctgcag cccagcagat tatgactgac
      421 tttggtggcc aatttccaaa tacctatgaa ggaatttcca gcttgaaagg gattggacct
      481 tacacagcag gagccatttc cagtattgct tttaacttgc ctgagccagc tgtagatggt
      541 aatgtcatgc gggtcttggc gcgtctgttt gaagtcaacc acgatattgg gattccaagt
      601 aatcgaaaaa tttttcaggc aatgatggag atcttgatta atccggatcg tccgggtgat
      661 tttaatcaag cattgatgga cttagggtct gatattgagt ctcctgtaaa tcccagacct
      721 gaagaaagcc cagtcaagga ctttagtgcg gcatatcaga atggaaccat ggaccgttat
      781 ccaattaaat ctcctaagaa aaagccggtt cctatttatc ttaaagcctt agttgtgaaa
      841 aattctcaag gacaattttt acttgaaaaa aatgaaagcg aaaagctctt ggcaggtttt
      901 tggcatttcc cctttataga agttgataac ttttcgcaag aagagcagtt tgacctcttt
      961 catcaggttg cggaagaaag tgtgaacttt ggtcccagtc cagaagagag tttccagcag
     1021 gactatgacc tagatgttga ttggcttgat gtttgttttg atacagtcca gcatgttttt
     1081 agtcatcgaa aatggcatgt acagattgta gcaggtcagg tgagtgactt ccatgatttt
     1141 tcagataggg aagttcgttg gctttcacca gaagaattca agaattaccc acttgccaaa
     1201 ccccaacaaa aaatctggca ggcttatgca caagccaact tagacagtag ccaagactag
     1261 ctattgtgtc ttctgactat ttttttggta taatagtaga gaaaaaggtg aacatatgaa
     1321 aaaaatacta attgtagatg atgagaaacc aatctcggat attatcaagt ttaatatgac
     1381 caaggaaggt tacgaagttg taactgcttt taatggtcgt gaagcgctag agcaatttga
     1441 agcagagcaa ccagatatta ttattctgga tttgatgctt ccagaaattg atggtttaga
     1501 agttgctaag accattcgta agacaagcag tgtgcccatt cttatgcttt cagccaaaga
     1561 tagtgaattt gataaggtta tcggtttgga acttggggca gatgactatg taacgaaacc
     1621 cttctccaat cgtgagttgc aggcgcgtgt taaagctctt ctgcgtcgtt ctcaacctat
     1681 gccagtagat ggtcaggaag cagatagtaa acctcaacct atccaaattg gggatttaga
     1741 aattgttcca gacgcctacg tggctaaaaa atatggcgaa gaactagact taacccatcg
     1801 tgaatttgag cttttgtatc atttagcatc gcatacaggt caagtcatca cgcgcgaaca
     1861 cttgcttgag actgtctggg gttatgacta ttttggtgat gtccgtacag ttgatgtgac
     1921 tgtacgacgt ctgcgtgaga agattgaaga tacgcccagc cgaccagagt atatcttgac
     1981 gcgccgtggt gtagggtatt acatgagaaa taatgcttga tttactgaaa caaaccattt
     2041 ttaccagaga ttttatcttt atcctgattt tgttaggttt catccttgtt gtgaccctct
     2101 tattactgga aaatagacgt gataatattc agttgaagca aatcaatcaa aaggttaaag
     2161 atttgattgc aggagattat tccaaggttc ttgatatgca aggtgggtct gaaatcacca
     2221 atattaccaa taatttgaat gacttgtcgg aggttattcg tctcactcag gaaaatctag
     2281 aacaagagag taagaggcta aatagtattc tgttttatat gacagatggg gttcttgcga
     2341 ccaaccgtcg gggtcagatt atcatgatta acgatacagc caagaagcaa ctggggttgg
     2401 ttaaggaaga tgttctgaat agaagcattt tggaattgct caagatagaa gaaaactatg
     2461 aattgcgtga tttgattacc caaagtccag aattgttgct agattcccaa gatatcaatg
     2521 gtgaatattt gaaccttcga gttcgctttg ccttgatacg tcgagagtct ggctttattt
     2581 caggtttggt ggctgttttg catgatacga cggagcagga gaaggaagaa cgcgaacgaa
     2641 gactctttgt ttccaatgtt agccatgagt tacggactcc tctgactagc gtaaaatcct
     2701 atcttgaagc cttggatgag ggggctttgt gtgaaactgt agcaccagac tttatcaagg
     2761 tttctcttga tgagaccaac cgtatgatgc gcatggtgac ggatctcctc catctttcac
     2821 gtattgataa tgctaccagt cacctagatg tggaactgat taacttcact gcttttatta
     2881 cctttatcct caatcgtttt gacaagatga aaggacagga aaaggagaaa aaatatgagt
     2941 tggtgagaga ttatcccatc aattctatct ggatggaaat tgatacagat aagatgacgc
     3001 aggttgtcga caatatttta aataatgcta ttaagtattc gccagatggg ggtaaaatca
     3061 ctgtcagaat gaagacaact gaagaccaga tgattttatc catttctgac cacggtttgg
     3121 ggattcctaa gcaggattta ccacgtatct ttgaccgttt ctatcgtgtg gatcgtgcta
     3181 gaagtcgtgc acaaggtggt acaggtctag gactgtctat cgctaaagaa attattaaac
     3241 aacataaggg ctttatttgg gccaagagtg aatacggcaa gggttcaacc tttaccattg
     3301 tgctccctta tgataaggat gcagtgaaag aagaagtatg ggaggatgaa gtagaagact
     3361 agaatgagta aaataggctt taaatacagt attttagcgt cgggttccag tggaaattct
     3421 ttttatctgg aaacctcaaa aaagaagctt ttagtggatg caggcttgtc tggcaagaaa
     3481 attaccagtc tgctagctga aattaaccgc aagccagaag acctggatgc catcttgatt
     3541 acccatgagc attcagatca tatccatgga gtaggcgttt tggctcgcaa gtatggtatg
     3601 gatctttatg ccaatgaaaa gacctggcaa gctatggaaa atagtaaata tcttggcaag
     3661 gtggattctt cgcaaaagca tatttttgaa atgggtaaaa ccaaaacttt tggagatatc
     3721 gacatcgaga gttttggtgt aagccatgat gcagtcgcac cgcagttcta tcgctttatg
     3781 aaggatgaca agagttttgt ccttttgaca gatacaggtt atgtcagtga ccgtatggcg
     3841 ggcattgtcg aaaatgcgga tggctatctt atcgaggcca accatgatgt agagattttg
     3901 cgatcaggtt cttacgcttg gcgactcaaa caacgaatcc tatctgacct tggtcacctt
     3961 tctaacgagg acggtgctga agctatgatt cggacgctag gaaatcgtac taagaagatt
     4021 taccttgggc atttatctaa ggaaaacaat atcaaggaac tggctcatat gaccatggtc
     4081 aatcagctgg ctcaagctga tctgggagtc ggagtagact ttaaggttta tgatacctca
     4141 ccagataccg caacaccatt gacagagata tagaaagaac gctgagaagg tgttcttttt
     4201 atattgactg aacacctaaa aagtaataca atggtgttac cattaaaaaa gggagcacaa
     4261 aagatgacta ctattacatt aaaagtttct gaagctgata aaacatttat gaaagcaatg
     4321 gctaagtttg aaggagtttc cctttcggaa cttattcgca ccaaaactct tgaagcccta
     4381 gaagatgaat acgatgctcg tgtggcagat ttagcctatc aagagtattt agaagacttg
     4441 gaaaaaggag ttgaacccat tacttgggaa gaaatgatgc atgatttagg cttgaaggat
     4501 gaataatttg tataaattag ttccaacaag acgtttcatc aagcaattga aaaaattgga
     4561 ccgttatacg cagaagctaa ttacaaacta tttacaaacc aatgttttgg aagacccaag
     4621 acgacacgga aaggctttgg ttggtaatcg cgttggtcaa tggcgctata gaattggtaa
     4681 ttatcgagtt atcgtacaaa ttgtagatga tgaattagtc gttgctactc tagaagttgg
     4741 tcatcggaga gatatttatt gaattaaagc tgagtaataa cagtatagaa agtaaggata
     4801 gtaaaaattt gaataaaaaa tggttatttg cagattatta tgatacaaca attattttac
     4861 ttgctttaat ctctgttatt ctggtattac ttggatttgc tgagatgatt gatttggaca
     4921 atccgcctta tagtattatt gatttagtaa tctggggtgt ttttgtaatt gattatagtt
     4981 ggcgcttttt tacgactaaa agaaaatggc gctttattct tgaaaatatt tttgatttat
     5041 tggctattct ccctttaaat gctattttta cagtgtttcg attagggcgt atctttcgtt
     5101 tagctaaact aacaaaatta ctgaaactga ctcgtttact tagaataatt ggacttacag
     5161 gtaaattaga aagaaaaatt agtagattct tacggacaaa tggcctgatt tatatcttgt
     5221 atgttaatat ctttattgtt ttagtaggga gttcgatttt atccgttgtt gaagaaaaat
     5281 ccttctcaaa tagtctttgg tgggctcttg tgacagtaac tactgttgga tatggggata
     5341 ttgttcctgc ttccattttt gggaaatggc tagcagtttt actgatgctt gtcggtattg
     5401 gaacaatagg gatgttaaca agtgctttga cgaacttttt tgtaaaggat aatccagatg
     5461 aacagataaa acttgataaa ctcaaagatg aattatcgag ccaaagaata ttactagaga
     5521 aacaatctga aaaaatcgaa gaactacatt gaatgataaa agatttagtt gaaaaaatat
     5581 cataaaggtg agacaaaaca aaacttgatt cgaggagaaa tcaagtaagt cttgacacaa
     5641 tagaacgcat attatcaggt ataaaaactt gatactatgc gttttataga gaaataaaat
     5701 aaaatctgaa caaatcaatt aggaatttaa agcattttat aacaacgttt tagagtgata
     5761 gggtgctatt tcaacttcaa cctactataa tacagaaaaa aatacaggaa aaaacaactc
     5821 cctgatgatt caaggagttg tctatagtta aattagtttt tagaagcttc ttggaattct
     5881 ggatttttcc atgcttcgtc aataatagct tgcaattctt tagcagatgc ttgcattttt
     5941 tgagtttctg catcgttcaa tggaatgttt actggacgaa cgataccatg tgcaccaaca
     6001 acagctggtt gaccgataaa gacattctca actccgtatt gaccttcttg gaatactgaa
     6061 agtggaagta ctgcgttttc atcgtcaagg attgctttag tgatacgagc aagggctact
     6121 gcgataccgt agtatgttgc accttttttg ttgatgattg tgtaggctgc atcacgaaca
     6181 ccttcgaaca attcaatcaa ttcagcttct tgaacatttt gagtgtcttt aagaaattct
     6241 tcaaggttta caccagcgat gttagcgtgt gaccaaacag cgaactcaga gtcaccgtgt
     6301 tcacccatga tgtaggcgtg cactgaacga gcatccacat ccaatttttc agcaagtgct
     6361 tgacggaaac gagctgagtc aagtgaagta cctgaaccga taacgcgttc tttagggaaa
     6421 ccagagaatt tccaagttga gtaagtcaaa acgtcaactg ggttagcagc aacaaggaag
     6481 atacctttga aaccagattc aacaacttga gttacgattg atttgttgat agcaaggttt
     6541 ttacctacaa ggtcaagacg agtttcacct ggtttttgag gtgcacctgc agtgatcaca
     6601 acaaggtcag cgtctgcaca gtcagagtat tgagctgcat agattttttt aggtgaagtg
     6661 aaggcaaggg cgtgactaag gtcaagcgca tcaccaacag ctttttcatg caattgtgga
     6721 atttcgataa ttccaagctc ttgtgcaatt ccttggttaa caagtgcaaa agcgtaagat
     6781 gaacctacag caccatcacc gacaaggata acttttttgt gttgtttagt tgaagtcatt
     6841 gttttaaaca tctccttaat tttattaggg gattttccct agacaacttc attctatcac
     6901 ttttaaaaaa ctttgtcacg aatatgcctt atagttctcg atgtaaacgt tttagtggtt
     6961 tagaggctga aatagatggg aatttatggt ataatgttgt tacttactaa ttgtgaaatg
     7021 aggcatttat taatgcagga taaaaattta gtgaatgtca atctgacaaa ggagatgaag
     7081 gcaagtttta tcgactacgc catgagtgtt atcgtagcgc gagctcttcc tgatgttcga
     7141 gatggcttaa aacctgttca ccgtcgcatt ctctacggaa tgaatgaatt gggtgtgacc
     7201 ccagacaaac cccataaaaa atctgctcgt attacagggg atgtcatggg taaatatcac
     7261 ccacacgggg attcctctat ttatgaagcc atggtccgta tggctcaatg gtggagctac
     7321 cgttacatgc ttgtagatgg tcatgggaat tttggttcca tggatggaga tagtgctgcc
     7381 gctcaacgtt ataccgaggc acgtatgagc aagattgctc tggaaatgct tcgtgatatc
     7441 aacaaaaata cagttgattt cgttgataac tatgatgcca atgaacggga acccttggtc
     7501 ttgccagcgc gttttccaaa ccttttggtt aatggagcaa ctggtatcgc ggttgggatg
     7561 gcaaccaata ttccacctca taatctgggt gaaaccattg atgcagtgaa gttggtcatg
     7621 gataatcctg aagtgactac caaggacttg atggaagtct tgcctggacc agattttcca
     7681 actggtgctc ttgtcatggg gaaatcaggt atccataagg cttatgaaac aggtaaaggt
     7741 tcgattgtcc tacgttctcg tacagagatt gaaacgacta agactggtcg tgagcgtatc
     7801 gttgtaacag aatttcctta catggtcaat aaaaccaagg tgcatgagca tattgttcgc
     7861 ttggttcagg aaaaacgcat tgagggtatc acagcagtac gtgatgagtc aaaccgtgaa
     7921 ggtgttcgat ttgttattga agtcaagcgc gacgcctcag ccaatgttat tctcaataac
     7981 ctcttcaaaa tgacccaaat gcaaaccaat tttggtttca atatgctcgc tatccaaaat
     8041 ggtataccga aaattttgtc tcttcgtcag attttggatg cttatatcga gcaccaaaaa
     8101 gaagtggttg ttcgtcgtac acgttttgat aaggaaaaag cggaagcgcg cgctcatatc
     8161 ttagaaggtc tcttgattgc gctagaccat atcgacgaag tgattcgtat catccgtgct
     8221 agtgaaacgg atgcggaagc tcaagctgag ttgatgagca agtttaagct ttctgaacgt
     8281 caaagtcaag ctatccttga tatgcgtctt cgtcgtttga caggtttgga acgcgataag
     8341 attcaatctg agtatgatga cctcttggct ctgattgcgg atttagcaga tattcttgct
     8401 aagcctgaac gtgtttctca aattatcaaa gacgaattgg atgaagttaa acgtaaattt
     8461 tctgataaac gccgtacaga gttgatggtt ggacaggtct tgagtctcga ggatgaggac
     8521 ttgattgaag aatcggatgt cttgattacc ctttctaaca gaggctacat taagcgtttg
     8581 gatcaggacg agttcactgc tcaaaaacgt gggggtcgtg gtgtccaagg aacgggagtg
     8641 aaagatgatg actttgttcg tgagttagtg tcaactagca cccatgatca tctgctcttc
     8701 ttcacaaaca agggacgtgt ctatcgtctt aaaggttatg aaattcctga gtatggtcgg
     8761 actgccaaag ggctaccagt agtcaatctc ttgaaattgg atgaagacga aagtattcag
     8821 acggttatca atgttgagtc tgatcgcagt gatgatgctt atctcttctt tacaacccgt
     8881 cacggtattg tgaagagaac cagtgttaag gagtttgcca atattcgtca aaatggtctc
     8941 aaagcgctga atttaaagga tgaagatgag ttaatcaatg tcttgttggc agaaggagat
     9001 atggatatta tcattggtac caagtttggt tatgcagttc gctttaatca atcagccgtt
     9061 cgtggtatga gccgtatcgc cactggtgtg aaaggtgtta accttcgtga aggagacaca
     9121 gttgttggtg ccagcttgat tactgatcaa gatgaggttc ttgttatcac agaaaaagga
     9181 tatggtaagc gtacagtcgc tactgaatac ccaacaaaag gtcgtggtgg taagggaatg
     9241 cagacagcta aaattaccga aaaaaatggc ttgctggccg gtcttatgac tgttcaaggg
     9301 gatgaggatt tgatgattat cactgataca ggtgtcatga ttcgaaccaa tcttgccaat
     9361 atttcacaaa caggacgtgc aactatggga gttaaagtaa tgcgcctgga tcaagatgct
     9421 cagatagtga ctttcacaac ggttgcggtg gcagaaaaag aagaagttgg gacagaaaac
     9481 gaaacagaag gtgaagcata atgtctcaaa aaaataataa aaagaaaaac aagcgaaaaa
     9541 atttgctgac aaatatccta gcaggatttc tgatattact gtcactggct ttgattttta
     9601 atactcaaat tcgaaatatt ttcatagtct ggaataccaa taagtatcaa gttagccagg
     9661 tatcaaaaga aaaattagaa gaaaatcagg atacagaagg caattttgac tttgattctg
     9721 tcaaagctat ctcttcggaa gctgttctaa cttctcaatg ggatgctcaa aaattaccag
     9781 ttattggggg aattgcaatt cctgaattgg aaatgaattt gccgattttt aaaggacttg
     9841 ataatgttaa tctcttctac ggagctggta caatgaaacg cgagcaagta atgggagaag
     9901 gaaattatag tctagctagt caccatatct ttggtgttga taatgctaat aaaatgttat
     9961 tttctccttt agataatgct aaaaatggca tgaagattta tctaaccgat aaaaataaag
    10021 tttatactta tgaaatacgt gaagtcaaac gtgtgacacc ggatcgtgtt gatgaagttg
    10081 atgatagaga tggggtcaat gaaatcacat tagtaacctg tgaagacctt gctgctacag
    10141 aacgtattat tgtcaaaggt gatttgaaag aaacaaaaga ttattcacaa acatctaatg
    10201 aaatcctaac ag
//
LOCUS       DAVKGC010000070        10075 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKGC010000070 DAVKGC010000000
VERSION     DAVKGC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.070"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            14..397
                     /locus_tag="VW049_001453"
     CDS             14..397
                     /locus_tag="VW049_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9432551.1"
                     /translation="MFVSYFNLLFSIDYINCLSETTDKNWHKSDRIFVTNTGKPVHSS
                     ILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSENKIPLKTITDRVGHSDSEVTTS
                     IYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(535..1839)
                     /gene="eno"
                     /locus_tag="VW049_001454"
     CDS             complement(535..1839)
                     /gene="eno"
                     /locus_tag="VW049_001454"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9432552.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2003..2449
                     /locus_tag="VW049_001455"
     CDS             2003..2449
                     /locus_tag="VW049_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9432553.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2446..3561)
                     /locus_tag="VW049_001456"
     CDS             complement(2446..3561)
                     /locus_tag="VW049_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU9432554.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVEYLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTYGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLDLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3570..4218)
                     /gene="serB"
                     /locus_tag="VW049_001457"
                     /pseudo
     CDS             complement(3570..4218)
                     /gene="serB"
                     /locus_tag="VW049_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
     gene            complement(4277..5710)
                     /gene="glgA"
                     /locus_tag="VW049_001458"
     CDS             complement(4277..5710)
                     /gene="glgA"
                     /locus_tag="VW049_001458"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9432555.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5707..6846)
                     /gene="glgD"
                     /locus_tag="VW049_001459"
     CDS             complement(5707..6846)
                     /gene="glgD"
                     /locus_tag="VW049_001459"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9432556.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     FRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEHGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6836..7978)
                     /locus_tag="VW049_001460"
     CDS             complement(6836..7978)
                     /locus_tag="VW049_001460"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9432557.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(7968..9896)
                     /gene="glgB"
                     /locus_tag="VW049_001461"
     CDS             complement(7968..9896)
                     /gene="glgB"
                     /locus_tag="VW049_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9432558.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFASLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3049 a         2231 c         1771 g         3024 t
ORIGIN      
        1 ttccctaatc aagctgttcg tatcttattt caatctacta ttttcaattg actatataaa
       61 ttgtctaagc gaaacaaccg ataagaattg gcacaaaagc gaccgtattt ttgttaccaa
      121 tacaggaaaa ccagttcata gttctatctt gagcaagtct ctccagcgag caaacgaacg
      181 ccttaaaaaa ccaattccca aacatctgtc ccctcacatc ttcagacaca ccactattag
      241 catcttatca gaaaataaaa ttcctttaaa aacaatcacg gacagggttg gtcattccga
      301 ctctgaagtc actacttcca tctacaccca cgtcacaaag aacatgaaag atgaagcaat
      361 caatgtactg gataaagtta tgaaaaagat tttttaaaaa gttttgcccc ttttttgtcc
      421 tctaaataca aaaatagccc ttcggataaa atccgagggg ctagaaacgt tgttaaatca
      481 acggccgaac ttttgaattt catggttcgg gataaaatag ttcactgaac tattttattt
      541 tttaaggttg tagaatgatt tcaatccacg atattcagct acttcaccaa gttggtcttc
      601 gatacgaagc aattggttgt atttagcgat gcggtctgta cgtgaaagtg aaccagtctt
      661 gatttgtcct gcgttagttg caactgcgat atcagcgatt gttgaatctt cagtttcacc
      721 tgaacggtgt gatacaacag cagtgtaacc agcttcttta gccatttcga tagcttcaaa
      781 agtttcagta agagtaccga tttggttaac tttgataagg attgagttag cagcaccttc
      841 ttggatacca cgtgcaaggt agtcagtgtt tgttacgaag aagtcgtcac caacaagttg
      901 tactttctta ccaagacgtt cagtaagagc tttccaacca tcccagtcgt tttcatccat
      961 accatcttca atagtgatga ttgggtattt gttaaccaat tcttcaaggt agtcgatttg
     1021 ttctgcagat gtacgaacag cagcaccttc accttcaaat ttagtgtagt cgtaaacttt
     1081 acgttcttta tcgtagaatt ctgatgaagc acagtcaaat ccaataaata cgtctttacc
     1141 tggtacatat ccagcagctt caatcgcagc aaggatagtt tcaacaccat cttcagttcc
     1201 ttcgaaacga ggagcgaatc caccttcgtc acctacggca gtttccaaac cacgtgattt
     1261 aaggattttc ttaagagcgt ggaagatttc agcaccgtaa cgaagggctt ctttaaatgt
     1321 tggcgcacca actggcaaga tcatgaactc ttggaaagcg attggagcgt cagagtgaga
     1381 accaccgttg atgatgttca tcattggagt tggaagaact ttagtgttga atccaccaag
     1441 gtagctgtaa agtgggattt caaggtagtc agcagcagca cgagctacag cgatagacac
     1501 accgaggatt gcattcgcac ccaatttacc tttgttagga gtaccgtcaa gtgcgatcat
     1561 agcacggtca atagcttgtt gatcacgtac atcgtagcca atgatagctt cagcaatgat
     1621 gttgtttacg ttgtcaacag ctttttgtgt accaagacca ccgtaacgag atttgtcacc
     1681 gtcgcgaagt tcaactgctt cgtgttcacc agtagaagct cctgatggaa ccataccacg
     1741 tccgaaagca cctgattcag tgtaaacttc tacttcaagt gttgggttac cgcgtgagtc
     1801 taggacttcg cgagcgtaaa catcagtaat aattgacatt ttttactctc cttatgagtt
     1861 aaatttttta cacctctata ataccttaaa acccctcctt tttcaagaaa aaacgttatc
     1921 tttgtgcaac ttttccttaa ctttataaag taatcgcttt cttttgtctg ttttattcta
     1981 acttttatga tatactgttt tcatgacaga tttatcaaaa caattacttg aaaaagctca
     2041 tggtgggtta aaaataaatc cggatgagca aagacgctat cttggtactt ttgaggaaag
     2101 agttcttgga tatgtagata ttgacacagc aaatagccct cagttagaaa aaggcttttt
     2161 atttatttta gaaaaccttc aggaaaaagc agagccacta tttgtgaaga tttcaccaac
     2221 tatcgaattt gataagcaag ttttctactt aaaagaagca aaagaaactg atagtcaagc
     2281 caccatagta tctgaagagc atattacttc tccttttggc ctggttattc atagcaatgc
     2341 accagttcaa gtagaagaaa aagaccttcg acttgctttt ccaaaacttt gggaagttaa
     2401 aaaggaagaa ccagccaaaa catccttatg gaagaaatgg tttagctaaa tcttgcacat
     2461 atttaataag tgcccaatat tggcagccgt gcgctccaga tagagactgg catttttcaa
     2521 actatcttct aaaggttcac ttttctccaa aatagaaaag acagcttgga tattttcaaa
     2581 tggtagggga ggtaaatctt cagcaagact accgcaaata gcaataacag gaactccaac
     2641 aggggttctt tttgcaacac ctataggcgc tttcccagca aagctttgac tatcaagtct
     2701 tccttctcca acaacaacca agtcagcatc tgaaactttc ttatcaaagt tgattaagtc
     2761 caagcaggta tcaattccag acacgatact tgcctgagca aaggcacaca aaccaccagc
     2821 aaggcctcca cctgctcctg ctcctttaat ttctaatgtt gcaggtgaga atttttcata
     2881 aaaatcttgg atcgcctgat ctacgactgc aaacatagtc ggatgtagac ctttttgatt
     2941 gccaaaagtg taagtcgcac cttgatgacc acataaggga ctcacgacat ctgctagaat
     3001 atgaatttga acaccttcag gaattttata gcaattttct gttgaaacag aagctaagtc
     3061 taataaggat tgaccggaag caggcaagac atttccatcc ctatcataaa attgataacc
     3121 taaaccagca gcaatcccca gtcctccatc attactggcc gtgccaccaa caccgatata
     3181 aatatcttta atccctttag aaatgagatg gagaatcaac tctccaatac cataagtttg
     3241 gatttgaagt ggatttcgtt tctctagcgg aatttttcca agaccaacca agtcagctac
     3301 ttcaaatagt gccagttccc ctttttgaaa atagcgcatg gcttcttttt gtccaaaagg
     3361 gtctgtcact tggatccatt tttcttttag gtcaagagaa tgtcggatag catctacagt
     3421 accttctccc ccatcaccaa cagggcagag gagatattct acatctgcta tcgattgttg
     3481 gaagcctctt tttattgctt cagctacctg ttgagctgtc aaactttcct taaacgaatc
     3541 tggtgcaatt acaatcttca tattttccct cattctaaac agtcaatcaa aggaagaact
     3601 tctaaaaaat ccctcttgtc aacatgatgt ggtatttctt ttttgagcac ttctttggca
     3661 caaaaggcga ttcctaactc cgccgacttc aacattaata gattattgac cccatcaccg
     3721 attgccaccg ttctttcttt agaaagtttt agtttctttc tccatttttc cagagtctct
     3781 tttttgacct ggggacttat aatttgttca actaattttc ctgttaaaag accttctttg
     3841 acttcaagct ggttggcagt gaaataggca ataccaaggg attttgctaa tctctccaac
     3901 tattggtatc caccagacac cacacaccag accaactagg atgccattct tttggagaat
     3961 agagatgaac tctgggacat ttagcgatag atgaattgag ttgaagacgt tatcaaagac
     4021 caaaatagga agaccttcca acaaggacac tctttttctt aaactgcttt caaagaccaa
     4081 ctctcctcgc attgcccgac ttgtaatctg cgaaatttcc gcctcatgac ctgcctctct
     4141 ccctaaaaga tcaatcactt cttctaggat taaggttcca tctacatcca aaacacacaa
     4201 gccttttact tgagacatca gttctcctct ctaaacagcc taaaaatcgt atgaagtcat
     4261 catacgattt tatctattaa ttaactaaac tatggtacaa gtcaaggtat gacttgcagg
     4321 ctgtatccca tgagaagtca ctctccatag cttgtttttg taggtttctc caaatgtctg
     4381 gatggtttct atacaagtcc aatgctgttt ggaaagtcca atttaaccaa taaggagata
     4441 gattgtcaaa gctaaagcca gtaccgcttc cttcgattgg attgaaagcg cgaactgtat
     4501 ctcgcaagcc tccaacttca tggaccaatg gcaaggttcc ataacgcata gccatcattt
     4561 gagacaagcc acacggttca aaacgacttg gcatgaggaa gaggtcacaa gcagcgtaga
     4621 tttcctgagc aagtttgaca tcaaaagtga tatttgttga tagcttgtct gggtaaatct
     4681 gagcaaacca tgagaaagct ccttcaaagg ctggatcgcc agttcccaaa agaacaatct
     4741 gaacatcttc ttgcaagata tggtgaagac tttcgaccac cacatcaaaa cctttttgac
     4801 gtgtcaaacg agaaacaatt cccaccagtg gaacgtctgc tctaacaggc aagccaactc
     4861 tttcttgcaa ttttgcctta tttttggctt tcccagacaa atcttcctga ttgaaatgat
     4921 agtctaaaag agcatccgtc tgaggattat aaagatcagc atcaatccca ttcacgatac
     4981 cagatacttt accagactcc attttaagaa tctgatccaa attacatcca aactgactag
     5041 tcataatttc atgagcatag ctaggtgaaa cggttgaaac acggttcgca tagagaatac
     5101 ctgccttcat ccagttcaga cagttgttcc atcgaagggt gccatcagcg taacgttcaa
     5161 agccaactcc aaacaaatca cccaacattc cttctgaaaa ttgtccttgg aattctaaat
     5221 tatgaatggt taaaactgtt tcaatgtcct cataggcttg aatccaacgg tatttttcct
     5281 tcaacaagaa aggaatcata gctgtatggt agtcatgaac atggagaaga tcaggaataa
     5341 agtcaatcct ttccatagcc tcaatggcag ccagttggaa aaaggcaaag cgttctccgt
     5401 catcaaaatc accgtaaaca tgaccacgga agaaataata ttgattgtca ataaagtaga
     5461 aggttacacc atttaatact gttttcttaa ttccacaata ctgtctgcgc caaccaacgc
     5521 tcacctcaaa atgaagtaca tcttcaatct gatttccaaa tttagcctct accatatcat
     5581 agtagggtaa aatcactgca acttcgtgcc cagcttttac cagtgatttt ggaagagcgc
     5641 caatgacgtc tcccaaacca cctgtttttg aaaagggtgc accctctgct gctacaaata
     5701 aaattttcat gaatgaatat cctctgttac tttagcacct ttcttaacca caactggatg
     5761 ttctgcagtt cctcgaatca caacaccatg ctcaacttca acccctttgt ccaagatagc
     5821 atattcgacc tgagcccctt ctccaataac aacacgaggg aagagcaggc tatctttaac
     5881 caagctatcc ttatggacat gaatattacg tgatagaaca gaattagcta cttgaccttc
     5941 aataatacta ccagaggcaa actgagaagt gcttacctta gatgtattag catagtaagt
     6001 tggctcttcg tttttgacct ttgtataaat cttttggttt ggtgagaaaa gagaatagaa
     6061 tttttgtgat tcaagcatat cgatattcgc ttgataataa gatttaacag agtgaatatt
     6121 ggctagatag cccgtgtact cgtaggcgaa agctccctct tttacagcca aatcccgtaa
     6181 aacatagcga aatttctctg gatgttcttt tttagcttct tcttccaagt gttcaatcaa
     6241 ccaaggtgta tcaacgacaa agatatctgt agacatattg aacgtttcag ctgttgactt
     6301 gctatcaaag agtttatgag aaagaacatg gtctgtttca tctacatcca agattgcatt
     6361 tacttctgaa atatctttct tagctagttt tttataaact acagtgatag gctcttttgt
     6421 tgtactatgt aggtggaaaa cttggttcaa atcaatgtta ataagaacat cgcagttgag
     6481 ggcaaccgtt tggtttgagc cagaacgttt caaataagta agaagctgtt ggtagtattc
     6541 ttttccaact gtactacttt ctacacgggt attgtaaatt cctagatagt aatgactaag
     6601 aagggttgat aagccccact cgcgtcctga acgaatatgg tcaaatactg agctgatatt
     6661 atcctgctgg aaaataccaa agacactacg aacacctgca ttagcaaggc ttgaaagtgg
     6721 gaagtcaatc aaacgatatt tcccaccaaa tggcaaactt gctactggac gatggtctgt
     6781 caatgtcgac atattgtgaa aaccaactgt atttcctaaa atggcagaat atttatcaat
     6841 cttcatctgt tgctaccccc actacttcat tatatcctac aacttgtact tcatctgttc
     6901 catcaatttc gacaccgtca gaaataatcg caccttcacc aataatggca cgtttaatct
     6961 tagctccttg accaatgata gctccactca tgataactga atcaaggact tccgctcctt
     7021 cgcgaacttg cgcgcctgtt gaaaggatag aatgtttaac agttccatca acgaaacatc
     7081 cgtctacaac taatgagtct tccacatgag catttgcccc gaggaagttt ggtggtgaaa
     7141 tcaagtttct tgagtaaatc ttccattgac ggttacgact atccaaggca ttttctggag
     7201 aaatatactc catgttcgct tcccaaagtg actcaatagt accaacatct ttccaataac
     7261 cactaaattc gtaagcataa acactttcac ctgactcaag gtaatttgga atgacatttt
     7321 taccaaagtc tgacatgcca accttgctct tttcagcagc gactaacata ttacgaaggc
     7381 gttgccaatc aaaaatgtag attcccatag aagcttttgt agatttaggt tgagctggtt
     7441 tttcttcaaa ttcaacaata cgattgttag catctgtgtt catgatacca aaacggcttg
     7501 cttctttaag agggacgtct aaaactgcta ctgtcaagct ggcattatta tccttatgag
     7561 actggagcat atcatcatag tccattttgt agatgtggtc cccagacaaa atcaagacat
     7621 actcaggatt gacactgtcg atatagtcga tattttggta aatagcgtga ctagttccct
     7681 caaaccaacg atttccttca cttgcagaat aaggttgaag aatagagacg cctgaattaa
     7741 taccgtctag tccccagctt gaaccattcc caatatggtt gttgagagca agtggttgat
     7801 actgtgtaac gaccccaaca ttatgaatcc ctgagttggc acagtttgat agggcaaagt
     7861 caatgatacg gtagcgccca ccaaattgca cagctggttt tgcgatgctt tgagtgagtt
     7921 taccgagacg agttccttgc ccaccagcaa gaatcaaagc taacatttca tttttcattt
     7981 tctactcctt tttggttttt atttgtgacg gttttagtag atttcaagcg acgtttgatt
     8041 ttccatacac ttgctcccat agccggtaga gtaaaggtta aggtctgctc ataatctttc
     8101 cataggcctt cttgcgtttg aacagtttga ttatgttctt tccaaacgcc tccccactct
     8161 tccaactcag tattccatac ttcttcgtaa attcctgcaa cgggtagtcc gattgtaaaa
     8221 tctttccgct caacaggtac catgttaaag atacagacta acatttctcc ctttttaccc
     8281 ttacgaataa aggaaagaac actctggtct cgattatccg catcaatgat ttcaatacca
     8341 tcatagctgg tatcgatttc ccacagacag cgatgatctt tgtaaaactg gtttagctga
     8401 gaagtgaaat acttcatctt agcattcatt gggtcttcta ggttagacca ttccaactgt
     8461 tcttcagatt tccattctag gaattgaccg tattcgctac ccatgaagag caatttctta
     8521 ccagggtgac aaatttggta cgtatagaga ttgcgcaagc ttgcgaattg attgtaacga
     8581 tctccccaca tcttatgcat catactcttc ttgccatgaa ccacttcatc gtgcgagaat
     8641 ggcaagagat aattctcctt gaaaacatac ataaagctga aagtcaccag gttaaagtca
     8701 tatttacgat aaatcggatc ttcttcgtag aaacggagga tatcattcat ccagcccatg
     8761 ttccatttgt agtcaaatcc tagaccacca atctctttca ttcccgtaat cttggtcgca
     8821 gacgaacttt cttctgcaat catcatcaca tctggatatt ctaacttaat aacttcattc
     8881 aagcgttgaa gaaaataata accttcatag ttgagatttc cgccatcttt attaggtgtc
     8941 catggagcat tatcatagtc caaatagagc atgttgctaa cagcatccac acgaatacca
     9001 tccaaatgat agacatcaat ccaatgctta atgcaagaaa ttaagaagga ctggacttca
     9061 ttttttccaa ggtcaaaatt aagggcaccc caaccatggt tatgagcctt attatggtct
     9121 tggtattcaa aagtcggtgt cccatcataa taggctaagg catcatcgtt gatggtaaag
     9181 tgacctggta cccagtccac aataacccca atattatggg tatgacactc ctcgacaaaa
     9241 tcttgaaact cctctggtct gccataagca tgctctaaag cgaagtaacc cataagctga
     9301 tacccccaac tcaagcccaa aggatgggac atcaagggca taaactcaat atgagtatag
     9361 ttcatttcaa cgagataagg aatgagttca tccttgagct gggcaaaact ataaggactg
     9421 ccatcagaat ttcttttcca tgatccagcg tgaacttcat aaatattgac aggacgctct
     9481 tcaaagcccc aacgttttct tcgtgccagc caaagtccat ccttccattt cttctcagga
     9541 agctctgtta agattgcccc tgttcctgga cgagcctcat acctgacagc aaaagggtca
     9601 atcttcatca gttgatgacc attttgacgt gtgacatgat atttgtaaat atgcccttct
     9661 tgagccatat tggtaaagac ttcccagacc ccaaaatcat ttcttaccat tggaatctga
     9721 ttttcaatcc agttggtaaa atcaccaacc aagtgaacag cctgagcatt aggtgcccaa
     9781 acacggaagg tatagccatg ctctccattt agttcttccc tatgtgctcc tagataatgt
     9841 tggagataaa aattttcacc cgtcataaag gtttttaatg cttctctatt atccatatac
     9901 tccccttctc ctgtaagcgt tttctatgtt tttattatac taccttttta gagaagattc
     9961 aagtaaatta ctatacttct ttaattattt tgaaaatcta caacaagttc acttactcgt
    10021 tcaattgtaa atcaatattt tttcaaaaaa ttgcgaaaac gcctttcttt ttcta
//
LOCUS       DAVKGC010000071        10019 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKGC010000071 DAVKGC010000000
VERSION     DAVKGC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10019
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.071"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            230..577
                     /gene="ssrA"
                     /locus_tag="VW049_001462"
     tmRNA           230..577
                     /gene="ssrA"
                     /locus_tag="VW049_001462"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(750..837)
                     /locus_tag="VW049_001463"
     tRNA            complement(750..837)
                     /locus_tag="VW049_001463"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Ser,seq:gga)
     gene            1000..1740
                     /locus_tag="VW049_001464"
     CDS             1000..1740
                     /locus_tag="VW049_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9432559.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1737..2648
                     /gene="pfkB"
                     /locus_tag="VW049_001465"
     CDS             1737..2648
                     /gene="pfkB"
                     /locus_tag="VW049_001465"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9432560.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTVEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2645..4597
                     /locus_tag="VW049_001466"
     CDS             2645..4597
                     /locus_tag="VW049_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9432561.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALSVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGSLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4761..6986
                     /locus_tag="VW049_001467"
     CDS             4761..6986
                     /locus_tag="VW049_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9432562.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILSQAELKFPEQEDDSDDEDVQVDFSAKEALKYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7029..7460)
                     /locus_tag="VW049_001468"
     CDS             complement(7029..7460)
                     /locus_tag="VW049_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432563.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENKSNQ"
     gene            7598..8740
                     /locus_tag="VW049_001469"
     CDS             7598..8740
                     /locus_tag="VW049_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9432564.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKIDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8749..9963
                     /gene="thiI"
                     /locus_tag="VW049_001470"
     CDS             8749..9963
                     /gene="thiI"
                     /locus_tag="VW049_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9432565.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
BASE COUNT         2903 a         1903 c         2285 g         2928 t
ORIGIN      
        1 ctatattttg gtgcaataag tgaaaagtag accgaataat ataaggattg attttatagt
       61 ttttaaactc aaatgaattg aaataaagag agtacgaaaa ttctcatctg aaagtatttt
      121 agaataattc tcttcgtgaa tttcttcaaa acagatagct tcatcttagg tatgtgattt
      181 ctttttgcat ttttgagtta gataaggtat aatggtttta ttgtcttttg gggtcgttac
      241 ggattcgaca ggcattatga ggcatatttt gcgactcgtg tggcgacgta aacgctcagt
      301 taaatataac tgcaaaaaat aacacttctt acgctctagc tgcctaaaaa ccagcaggcg
      361 tgacccgatt tggattgctc gtgttcaatg acaggtctta ttattagcga gatacgatta
      421 agccttgtct agcggtttga taagagattg atagactcgc agtttctaga cttgagttat
      481 gtgtcgaggg gctgttaaaa taatacataa cctatggttg tagacaaata tgttggcagg
      541 tgtttggacg tgggttcgac tcccaccggc tccattattc ctttgcattc cttggtaaaa
      601 cgttgttaaa tcaacgtttt ttatttttat ctttggtatt cctttgcatt cttttgctaa
      661 aaagggagtc acaaacagac cctattttaa aaaaaggata gaaaaaagga tacaacattt
      721 gtcgcatcct aaaaataatc ttttttcgac ggaagacatg ggattcgaac ccacgcacgc
      781 tattacacgc ctaccgcgtt tccaacacgg cctcttaagc ctcttgagta atcttccaat
      841 acttactcaa atagtctacc ataaaggctc ttatcttgca ataaaaattc tagaaataag
      901 aaaaaatgat agattttgaa agaaaatgat aaaaaatgct tgacttcgaa agaaagtatg
      961 atagaatgaa tagtgtaaac gataacagga ggtgattcag tgttaaaaac agaacgtaaa
     1021 caactaattt tagaggagtt aaatcagcat catgtagttt ctctagaaaa attagttagt
     1081 ttgctagaaa cgtcagaatc aacggttcga agagacttgg atgagttgga agcggaaaac
     1141 aagcttcgtc gtgtgcatgg tggagcagaa ctcccccact ccttacagga agaagaaacc
     1201 attcaagaaa aatctgtcaa aaaccttcaa gaaaagaagt tgctggctca gaaagcagcc
     1261 tctctcatta aagaaaaaga tgtcatcttt atcgatgctg gaacaacaac tgcttttttg
     1321 attcatgaat tggtcaataa gaatgttaca gttgtgacca actccattca ccatgccgct
     1381 cagttggttg aaaagcagat tccaactgtc atggttggag gaaacgtcaa gacggcgaca
     1441 gatgctagta tcgggggcgt tgctcttaac cagattaacc aattgcactt tgaccgtgcc
     1501 tttatcggaa tgaatggtgt tgacgatggc tattatacga ctcctgatat ggaggaggga
     1561 gctgtgaaaa gagctatttt ggagaatgcc aagcagacct acgtcttggt ggattcgtca
     1621 aaaattggac aaacttgctt tgccaaggta gcaccactca aacgcgctat cgttatcact
     1681 agtcaagggc atgagctctt gcaggttatt aaggagaaaa cggaggtaat agaagtatga
     1741 tttatacagt cacactcaat ccatccattg actatatcgt tcgtttggac caagtcaaag
     1801 ttggtagtgt caatcgtatg gacagtgatg ataagtttgc tggtgggaaa ggaatcaatg
     1861 tcagccgtgt cttgaaacgt ttgaatatac caaatacagc gacgggattt atcggtggct
     1921 ttactggtaa atttatcaca gatactctag cagaggaaga aatcgagaca cgttttgtcc
     1981 aagtggcaga agatactcgt atcaatgtta aaatcaaagc agaccaagaa acagaaatca
     2041 acggaacggg tccaactgtt gaaccggttc agctagaaga attgaaagct attttatcta
     2101 gtctgacagt agaagataca gttgtctttg caggttcaag tgctaaaaat ctaggcaatg
     2161 ttatctataa ggatttgatt tccttgacgc gccagactgg tgcgcaagtg gtctgtgact
     2221 ttgaaggaca gaccttaatt gatagtttgg actaccagcc tcttcttgta aaaccaaaca
     2281 atcatgaact tggagcgatt tttggggtta aactcgaaag tttagatgaa attgagaaat
     2341 acgctcgtga gttactggct aagggtgctc aaaatgttat tatctctatg gctggtgatg
     2401 gcgcccttct tgtcacatct gagggagctt acttcgctaa accaatcaaa ggaacagtca
     2461 aaaattcagt tggagctggt gattctatgg ttgctggatt cacaggtgaa tttgtcaaat
     2521 caaaagacgc agtagaagcc ttcaaatggg gagtggcttg cggaacggca actaccttct
     2581 cagatgactt ggcaacggcg gaatttatta aagaaacata tggaaaagtt gaggtagaaa
     2641 aaagatgaaa attcaagacc tattgagaaa agatgtcatg ttgctagatt tgcaggcaac
     2701 tgaaaaaaca gctgtcatcg acgagatgat taaaaatttg acagagcacg gttatgtaac
     2761 agattttgaa acatttaaag aaggaatttt ggcgcgtgaa gctttgactt ctactggttt
     2821 gggtgatgga atcgcaatgc ctcacagcaa aaacgctgct gtcaaagaag cgacagttct
     2881 atttgctaag tcaaataagg gtgttgacta cgagagcttg gatggacaag caactgacct
     2941 cttcttcatg attgcagctc cagaaggtgc caatgatact cacttggcag ccttggcaga
     3001 attgtctcaa tacttgatga aagacggttt tgcagacaaa cttcgtcaag caacatctgc
     3061 agaccaagtt atcgaacttt ttgaccaagc ttcagaaaaa actgaggaac ttgttcaagc
     3121 acctgctaat gactctggtg actttatcgt agctgttaca gcttgtacaa caggtattgc
     3181 ccacacttac atggcccaag aagcccttca aaaagtagct gctgaaatgg gggttggtat
     3241 caaggtcgaa accaacggtg ctagcggtgt tggaaatcaa ctaactgcag aagatatccg
     3301 taaggctaaa gctattatca ttgcagcaga caaggccgtt gaaatggatc gatttgatgg
     3361 aaaaccattg atcaatcgtc cagttgctga cggtatccgt aagacagaag agctaattaa
     3421 cttggctctt tcaggagata ctgaagtcta ccgtgccgct aatggtgcaa aagctgcaac
     3481 agcctctaac gaaaaacaaa gccttggtgg tgccttctac aaacacttga tgagtggtgt
     3541 atctcaaatg ttaccattcg ttatcggtgg tggtatcatg attgcccttg ccttcttgat
     3601 tgacggtgct ttgagtgttc caaatgaaaa ccttggcaat cttggttctt accatgagtt
     3661 agcttctatg ttcatgaaaa ttggtggagc tgcctttggt ttgatgcttc cagtctttgc
     3721 gggttatgtt gcctactcta ttgctgaaaa accaggtttg gtagcaggtt tcgtggctgg
     3781 tgctattgcc aaagaaggtt ttgcctttgg taaaattcct tatgccgcag gtggtgaagc
     3841 aacttcaact cttgcaggtg tctcatctgg tttcctaggt gcccttgttg gtggatttat
     3901 cgcaggtgcc ttggttcttg ccatcaagaa atacgttaaa gttcctcgtt cactcgaagg
     3961 tgctaaatca atccttctat tgccacttct tggaacaatc ttgacaggat ttgttatgtt
     4021 agctgtgaat atcccaatgg ctgcaatcaa cactgctatg aatgacttcc taggcagtct
     4081 tggaggaggt tcagctgtcc ttcttggtat cgtccttggt ggaatgatgg ctgttgacat
     4141 gggtggacca gttaataaag cagcttatgt ctttggtaca ggtacgcttg cagcaactgt
     4201 ttcttcaggt ggttctgtag ccatggcagc agttatggct ggaggaatgg tgccaccact
     4261 tgcaatattt gtcgcaactc ttcttttcaa agataaattt actaaggaag aacgtaactc
     4321 tggtttgaca aacatcatca tgggcttgtc atttatcact gagggagcga ttccatttgg
     4381 tgccgctgac ccagctcgtg cgattccaag cttcatcctt ggttcagcag tagcaggtgg
     4441 actcgttggt cttactggta tcaaactcat ggcgccacac ggaggaatct tcgttatcgc
     4501 ccttacttca aatgctctcc tttacctcgt ttctgtcttg gtaggagcaa tcgtaagtgg
     4561 tgtggtttat ggttacctac gcaaaccaca agcataaaaa atagaaaaat gaaaagattg
     4621 gaccgtttgg tgcagtcttt ttctcttccc gaaatgcctg tgaaatatgg tataatagaa
     4681 gaatggcaaa caagaataca agtacaacaa gacggagacc gtctaaagca gaactggaaa
     4741 gaaaagaagc gattcaacga atgttgattt cgttaggaat tgcgatttta ttgattttcg
     4801 cagccttcaa attaggggct gcaggtataa ccctttataa tttaattcgc ttgctagtgg
     4861 gtagcctagc ttatctggcg atattcggcc tattaatcta tctcttcttt ttcaagtgga
     4921 tacgaaaaca ggaaggactc ttatctggct ttttcaccat atttgctggc ttactcttga
     4981 tttttgaggc ctacttggtt tggaaatatg gtttggacaa gtccgttcta aaagggacca
     5041 tggctcaggt tgtgacagat ctgactggtt ttcgaacgac tagctttgct ggagggggct
     5101 tgatcggggt cgctctttat attccaacag cctttctctt ttcaaatatc ggaacttact
     5161 ttattggttc tatcttgatt ttagtgggtt ctctcctagt cagcccttgg tctgtttacg
     5221 atattgctga atttttcagt agaggctttg ccaaatggtg ggaagggcac gagcgtcgaa
     5281 aagaggaacg ctttgtcaaa caagaagaaa aagctcgcca aaaggctgag aaagaggcta
     5341 gattagaaca agaagagact gaaaaagcct tactcgattt gcctcctgtt gatatggaaa
     5401 cgggtgaaat tctgacagag gaagctgttc aaaatcttcc acctattcca gaagaaaagt
     5461 gggtggaacc agaaatcatc ctgtctcaag ctgaacttaa attccctgaa caggaagatg
     5521 actcagatga cgaagatgtt caggtcgatt tttcagccaa agaagccctt aaatacaaac
     5581 ttccaagctt acaactcttt gcaccagata aaccaaaaga tcagtctaaa gagaagaaaa
     5641 ttgtcagaga aaatatcaaa atcttagaag caacctttgc tagctttggt attaaggtaa
     5701 cagttgaacg ggccgaaatt gggccatcag tgaccaagta tgaagtcaag ccggctgttg
     5761 gtgtaagggt caaccgcatt tccaatctat cagatgacct cgctctagcc ttggctgcca
     5821 aagatgtccg gattgaagca ccaatccctg ggaaatccct aatcggaatt gaagtgccca
     5881 actccgatat tgccactgta tctttccgag aactatggga acaatcgcaa acgaaagcag
     5941 aaaatttctt ggaaattcct ttagggaagg ctgttaatgg aaccgcaaga gcttttgacc
     6001 tttctaaaat gccccacttg ctagttgcag gttcaacggg ttcagggaag tcagtagcag
     6061 ttaacggcat tattgctagc attctcatga aggcgagacc agatcaagtt aaatttatga
     6121 tggtcgatcc caagatggtt gagttatctg tttacaatga tattccccac ctcttgattc
     6181 cagtcgtgac caatccacgc aaagccagca aggctctgca aaaggttgtg gatgaaatgg
     6241 aaaaccgtta tgaactcttt gccaaggtgg gagttcggaa tattgcaggt tttaatgcca
     6301 aggtagaaga gttcaattcc cagtctgagt acaagcaaat tccgctacca ttcattgtcg
     6361 tgattgtgga tgagttggct gacctcatga tggtggccag caaggaagtg gaagatgcta
     6421 tcatccgtct tgggcagaag gcgcgtgctg caggtatcca catgattctt gcaactcagc
     6481 gtccatctgt tgatgtcatc tctggtttga ttaaggccaa tgttccatct cgtgtagcat
     6541 ttgcggtttc atcaggaaca gactcccgta cgattttgga tgaaaatgga gcagaaaaac
     6601 ttcttggtcg aggagacatg ctctttaaac cgattgatga aaatcatcca gttcgtctcc
     6661 aaggctcctt tatctcggat gacgatgttg agcgcattgt gaacttcatc aagactcagg
     6721 cagatgcaga ctacgatgag agttttgatc caggtgaggt ttctgaaaat gaaggagaat
     6781 tttcggatgg agatgctggt ggtgatccgc tttttgaaga agctaagtct ttggttatcg
     6841 aaacacagaa agccagtgca tccatgattc agcgtcgttt gtcagttgga tttaaccgtg
     6901 cgacccgtct catggaagag cttgagatgg caggtgtcat tggtccagct gaaggtacca
     6961 agccaagaaa agtattgcag caataaaaaa tagcttcttt ccaaggttgg aaggagctat
     7021 tttactggct attgattact tttattttct gaagttggag tattgggctg tttttcattt
     7081 tcagtggcag gtttacttgt agcaggtgta gtagagtcct gagttgctgt tttctgatct
     7141 tcttttttct cttccttgac gctagatttt ggcgtttctt cttgttgtgt tttttcttga
     7201 gaagtgttat tttccttatt tggagtagta ttttcctgag gggattcttt ttgaatttct
     7261 ttgacaaagg ttgtcgtctg gcttgtcgta ggttcttttt tagtattttt attattatta
     7321 tccaaggcgt tcatgatcgt gatacccgct gtaatcaaac caaggatact accgatagta
     7381 gtaatggttt tttgaaaaac agtaaagcct tttttgatat gttctgtttt cggttttgaa
     7441 gttctacgat agttagacat acattctctc ctttgattat gatttattat actctgtttc
     7501 tttaaaaaaa tcttaaaaaa agagaaattg ccctttcttg tcgcaagctc cgtttcatga
     7561 tacaatagag ggagtgattt tagaaagcgt gagaaacatg atttactttg ataattcagc
     7621 gacgaccaag ccttatcctg aagcccttga aacctatatg caggtcgctt caaaaatttt
     7681 aggaaaccca tctagtctcc atcgtttggg agaccaagca acacgaatct tagatgcttc
     7741 tcgccaacag attgcagatt taatcggtaa gaaaatcgat gaaatcttct ttacctctgg
     7801 tggaacagaa ggggataact gggttatcaa gggtgtggcc tttgaaaaag ctcagtttgg
     7861 caagcacatc attgtttcag ccattgaaca tccagcagtc aaagagtcag ccctctggtt
     7921 gaaaagtcaa ggatttgaag tggattttgc tccagttgat aagaaaggct tggtcgatgt
     7981 tgaggcgtta gcaggtttga tacggcctga tacaatcctc gtttccatca tggctgtgaa
     8041 caatgaaatc ggctctatcc aacctattga ggctatttca gaattcttgg cagacaagcc
     8101 gactatttcc ttccacgttg atgcggttca ggcgcttgcc aaaattccga ctgaaaagta
     8161 tctgacagaa cgggtggatt gcgcgacctt ctcgagtcat aaatttcatg gtgtccgtgg
     8221 tgttggcttt gtctatatca aatctggcaa gaagattaca cctcttctta caggtggtgg
     8281 ccaggagcga gattatcgtt cgacaactga aaatgtggca gggattgcag cgacagccaa
     8341 ggctctccgt ttatctatgg aaaagctaga tatctttagg agcaagactg ggcagatgaa
     8401 ggcagtgatt caccaagctc ttctgaacta tccggatatt tttgtctttt cagatgagga
     8461 agactttgca cctcatattc tgacttttgg aatcaaaggt gttcgaggtg aagtcatcgt
     8521 tcacgccttt gaagactatg atattttcat ctcaacaacc tcagcttgtt catctaaggc
     8581 aggaaaacca gccggtacct tgattgccat gggagtggac aaagataagg ccaagtcagc
     8641 tgtgcgtctt agcctagact tggaaaatga tatgagtcag gtcgagcagt ttttgaccaa
     8701 gttaaaattg atttacaatc aaactagaaa agtaagatag gagcattcat gcagtattca
     8761 gaaattatga ttcgctacgg agagttgtca accaagggta aaaaccgtat gcgtttcatc
     8821 aataaacttc gtaataatat ttcggacgtt ttgtctatct atacccaagt taaggtaaca
     8881 gcagatcgcg accgtgccca cgcttacctc aatggagctg attacacagc agttgcagaa
     8941 tctctcaaac aagtttttgg aattcaaaac ttttctcctg tttataaggt tgaaaaatct
     9001 gtagaagttt tgaagtcttc tgtccaagag attatgcggg acatctacaa ggaaggtatg
     9061 acctttaaga tttctagcaa gcgtagcgac cacaactttg aacttgatag tcgtgaactc
     9121 aaccaaacac ttggaggggc tgtattcgaa gccattccaa atgtgcaagt tcaaatgaaa
     9181 agtcctgaca tcaatcttca ggtggagatt cgtgaagaag cagcctatct ttcttatgaa
     9241 accattcgtg gggctggtgg tttgccagtt ggaacttcag gtaaagggat gctcatgttg
     9301 tcaggaggga ttgactcacc tgtagcaggt tatcttgctc ttaagcgtgg ggtggatatc
     9361 gaggcagttc actttgctag tccaccatat actagtcctg gtgccctcaa gaaagcgcag
     9421 gacttgaccc gtaaattgac caagtttggc ggaaatatcc agtttataga ggtgcctttc
     9481 acagagattc aagaggaaat caaagccaaa gcgccagaag cttatttgat gactctaact
     9541 cgtcgcttta tgatgcggat tactgaccgt attcgtgagg tacgaaatgg tttggttatc
     9601 atcaatgggg aaagtctagg tcaagtagcc agccaaaccc ttgaaagtat gaaggctatc
     9661 aatgctgtta ccaacactcc catcattcgt cctgtggtta ccatggacaa gttggaaatc
     9721 attgacatcg cccaggaaat cgataccttt gacatttcaa tccaaccgtt tgaagactgt
     9781 tgtaccattt ttgcaccaga tcgtccaaaa acaaatccta aaattaagaa tgcggagcag
     9841 tacgaagcgc gtatggatgt tgaaggcttg gttgagcgag cagtggctgg aatcatgatt
     9901 actgaaatca cacctcaagc cgaaaaagat gaagttgatg acttgattga taatctactc
     9961 taattcagaa aatccaaaag aatagcgaaa atcagtaaaa aaagttagtt ttttctcta
//
LOCUS       DAVKGC010000072         9972 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKGC010000072 DAVKGC010000000
VERSION     DAVKGC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.072"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            1..184
                     /locus_tag="VW049_001471"
                     /pseudo
     CDS             1..184
                     /locus_tag="VW049_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            267..650
                     /locus_tag="VW049_001472"
     CDS             267..650
                     /locus_tag="VW049_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9432566.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            702..1391
                     /locus_tag="VW049_001473"
     CDS             702..1391
                     /locus_tag="VW049_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9432567.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1433..1666
                     /locus_tag="VW049_001474"
     CDS             1433..1666
                     /locus_tag="VW049_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432568.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            1817..2428
                     /locus_tag="VW049_001475"
     CDS             1817..2428
                     /locus_tag="VW049_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9432569.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            2589..3383
                     /locus_tag="VW049_001476"
     CDS             2589..3383
                     /locus_tag="VW049_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9432570.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3380..4015
                     /gene="trmB"
                     /locus_tag="VW049_001477"
     CDS             3380..4015
                     /gene="trmB"
                     /locus_tag="VW049_001477"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9432571.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4141..4620
                     /gene="rimP"
                     /locus_tag="VW049_001478"
     CDS             4141..4620
                     /gene="rimP"
                     /locus_tag="VW049_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9432572.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVRIPYSLVSKARLAVKL"
     gene            4664..5800
                     /gene="nusA"
                     /locus_tag="VW049_001479"
     CDS             4664..5800
                     /gene="nusA"
                     /locus_tag="VW049_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9432573.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5822..6115
                     /locus_tag="VW049_001480"
     CDS             5822..6115
                     /locus_tag="VW049_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9432574.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6108..6407
                     /locus_tag="VW049_001481"
     CDS             6108..6407
                     /locus_tag="VW049_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9432575.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLAKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6424..9216
                     /gene="infB"
                     /locus_tag="VW049_001482"
     CDS             6424..9216
                     /gene="infB"
                     /locus_tag="VW049_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9432576.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKTEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9467..9817
                     /gene="rbfA"
                     /locus_tag="VW049_001483"
     CDS             9467..9817
                     /gene="rbfA"
                     /locus_tag="VW049_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9432577.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3027 a         1837 c         2283 g         2825 t
ORIGIN      
        1 tggttagtcc tagaaccaat caacttatgt ttatcggctt ggcagatttt atgtttgtca
       61 tttgtttgta tagaggtatt tcagaaacag aattctatca acaactaatc gcttatatag
      121 gtgtattctc tgcttgttta tcacgcttct gctcatgtgg ggcttgaagt attataaaaa
      181 gtagctttct atccttttct gacaggatat ttgagtagaa ggctctatta aattgttagt
      241 gaaataaatg aatggaggta tttaatatga aatacagatt attttttgtt attttcttga
      301 gtagtgtgtt ggatattctt ttagggacat ttttacaaat ctctatcgta tctattgggt
      361 ggcttgttct ttacagtggt ttgtttgaag caggagtttt ccttcttgct aataaagggg
      421 tggcggtaaa aatcaaggaa gtagatattc gaaatcgctt taaatttatt tttggaaaaa
      481 ccttatggtt tcaaattctt ttgctcatct ttttgataat caaactttat cttggtttgg
      541 atgcgaggtt gattttattc tatggacata ttttcattgt ctttaatgcc ttaatgtatc
      601 tattatctag tagtcaggtt agccttaaaa aaaacaaact gtcttcttaa tcttacagca
      661 gtagagcctg acaaggtgag gtcatagtag ggaggatttc tatgaaaaag tatcaacttc
      721 tattcaaaat aagtgcagtt ttctcttact tatttttcgt atttggtctt tctcagatga
      781 cgcttattat tcaaaattat tggcaatttt cttcccagat tggcaatttc gtctggattc
      841 aaaatttctt gagtttgcta tttagcggag tcatgatttg gattctggtt aagacaggtc
      901 atggttatct ctttcacatt ccaagaaaaa aatggctttg gtattcgatt ttgacagtat
      961 tagtggtagt gctccagatc tcttttaacg ttcagacagc taaacatgtt cagtcaactg
     1021 ctgaaggttg ggctgtattg atcggttata gtgggactaa ctttgcagag ctaggtattt
     1081 atatagccct gttctttctg gttccactga tggaagaatt gatttataga ggattactgc
     1141 aacatgcttt ctttaagcat tcgcgatttg gtcttgattt gcttcttcct tctattttat
     1201 ttgctctccc tcatttttca agcctgccta gtctgttaga tatcttcgtc tttgcaacag
     1261 ttggaatcat ctttgctggt ttgacccgct ataccaagag catttatcca tcctatgcgg
     1321 tgcatgtgat caataatatt gtagcgacct tcccgttttt gctcactttt ctacataggg
     1381 tcttggggta aaaaaacaaa aggcttgctt ttcagccata gaggaggtca tcatgtataa
     1441 acacttattt ttcctagatt ccaaaacttt agatcggttg acaccctata ttctagtctt
     1501 ggcttctgac accattgctt ttaatgtttt tgtgctaacc tttgtatctg cggtggtttt
     1561 taatttccta aattccatgc tagctttaat ggctatattc ataggggctg gctatgtggt
     1621 cggattttgg ttactaatac tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag
     1681 gctgtacttg agtacggcaa ggcgacgttg acgtggtttg aatttgattt tcgaagagta
     1741 taaaatggtt tgttttggaa aggctagaac taaagaatga cgtgtaggaa gtctgtttgt
     1801 tgaggaggat aattttatgg agttttttga taaatttcat gccttgtgtt ttggattttt
     1861 agtactaata attgtcatta cagttcctta tacgattaac catgggggtt tttttcaaaa
     1921 tgaatctgca ttgattcttg taagtcttct tgtaacctcg ctgagtgttg cttatgctag
     1981 aaagtttgaa atgatttctt ttgggatgtt aagcaagaaa caacttttgc ttttcattgc
     2041 aatctttctt ctaagtgtac ttgagacgct ggtttatatt catttcttcg ctgtttcttc
     2101 tggctcaggg gtccaacact tggcggaagt cagcagagga atttccctgt ctttgatttt
     2161 aactacctca gtttttggcc ccatccagga ggaactcatt ttcagaggac ttcttcaagg
     2221 tgcggttttt gacaattctt ggttagggct tgtgctaact tcctctctct tttctttcat
     2281 gcatggacct tctaatgtcc cttcgtttat tttttatcta cttgggggtt tgttgctggg
     2341 ctttgcttat aaaaagagtc aaaacctatg ggtttctact ctagtccata tgctttacaa
     2401 cagttggcca ctcttatatt atttataaaa attatgaaaa ggtaagcaga agacggtgct
     2461 tacctttttc aagctataat gatacccaag ccaatccaga ggcttttctt tgagaatcgg
     2521 gttagagtgg ttgacgaata ggccaaaaac tagtagaata gtaaggaaac tttatacgga
     2581 ggaaagaaat ggatttgggt gataatgagc taacactgac tcccatacct gggaaaagtg
     2641 gcaaggctta tatgggtagc tatcctgatg ggaagcgcat ctttgtaaaa atgaacacct
     2701 ctccaatcct acctggtcta gctagagaac aaattgctcc acaattatta tggagtcgtc
     2761 gtttggcaga tgggcgtgat atgtgtgctc aagaatggtt gacaggcaag atattgaccc
     2821 cctatgatat gaatcgtaag caaatcgtca atattttaac ccgtcttcat cgctcacgtc
     2881 cgttgatgac acaattgagt cgtttgggct atgccatgga aacacctgta gatttactac
     2941 agtcttggca ggaaacggct ccagatgctt tgcgtaaaaa tcattttatc agtgaagtga
     3001 tggctgattt acgtcagact attccaggat ttagagagga ccatgcgacc attgtccatg
     3061 gagatgtacg acatagtaat tggattgaga cagatagtgg cttgatttat ttagtagatt
     3121 gggattcggt tcgcttgacc gatcgcatgt ttgatgtggc ccatatgctc tgccattata
     3181 tttcagaaca tcagtggaag gaatggttga cctactacgg ttacaagtac aatcaaacgg
     3241 tattaagtaa attgtattgg tatggtcaat tgtcttattt gagtcagatt tccaagtatt
     3301 atatgaacca agatttagaa aatgtcaatc gggagattca tggtttgcgt catttccgag
     3361 acaagtatgg aaagagaaga tgagagttag aaatcgtaaa ggggcaacag aattactaga
     3421 ggcaaatccc cagtatgtgg tcctcaatcc cttggaagcc aaggcaaaat ggcgggactt
     3481 gtttggcaat gataatccca ttcatgtgga agttggaagt ggaaagggtg cctttgtttc
     3541 aggtatggcc aagcaaaacc ctgacatcaa ctatatcggg attgatattc aaaagtctgt
     3601 tttgagctac gctttggaca aggtgcttga agttggagtg cctaacatca agctcttgtg
     3661 ggtagatggt tctgacttaa ctgactactt tgaagacggt gagattgatc gcttgtatct
     3721 gaacttttca gatccatggc cgaaaaaacg ccatgaaaag cgtcgtttga cctacaagac
     3781 ctttttggac accttcaaac gtatcttgcc tgaaaatgga gaaattcatt tcaagacgga
     3841 taaccgtggc ttgtttgagt acagtttagt gagcttttct caatatggca tgaaactcaa
     3901 tggtgtctgg ctagatttgc atgccagtga ttttgaaggc aatgtcatga cagaatacga
     3961 gcaaaaattc tcaaacaagg ggcaagttat ctaccgagtt gaggcagaat tttaagagat
     4021 agcctaaaat taggctgtac aagtgctttt gctttacata agttggcaaa cgtgctatac
     4081 tgatagtaag aatatgaaaa gtgaggcggg gaaatatctt cgcctcttgc ttatgaggag
     4141 gtggacgcaa tcgcaacaat cgtagaatta gtcagagaag ttgtagaacc tgtcatagaa
     4201 gctccttttg aactcgtgga tatcgagtat ggaaagattg gcagtgacat gattctcagt
     4261 atttttgtag ataaacccga aggaattacc ttgaacgaca cggcagactt gacagaaatt
     4321 atcagtcctg tcctagacac catcaagcca gatcccttcc cagaacaata tttcctagaa
     4381 attaccagtc caggcttgga acgtcctttg aaaaccaagg atgccgtcgc tggagcggtt
     4441 ggaaaataca tccatgtcgg actctaccaa gccatcgata agcaaaaggt ctttgaagga
     4501 actttgttgg ccttcgaaga ggacgagttg actatggaat atatggacaa gacgcgtaag
     4561 aaaaccgtcc gaattccata cagtttagta tcaaaagcac gtttagcagt taaattatag
     4621 aaaaagaaag gatagctttt gaggattcaa aagtgaagaa aacatgagta aagaaatgct
     4681 agaggccttc cgcattttgg aagaagacaa gggaatcaaa aaagaagata tcatcgacgc
     4741 agtagtagag tcgcttcgtt ccgcttatcg cagacgctat ggtcagtcag acagcgtagc
     4801 tattgacttc aacgaaaaaa caggtgactt tacagtttat actgtccgtg aagttgttga
     4861 tgaagtattt gatagccgtt tggaaatcag cttgaaagat gctcttgcca ttaattcagc
     4921 ttatgaactt ggagacaaaa tcaagtttga agaagcacca gctgagtttg gtcgtgtagc
     4981 agcccaatct gccaaacaaa ccatcatgga aaaaatgcgc aagcaaacac gtgccatcac
     5041 ttacaatact tacaaagaac atgagcaaga aatcatgtct ggtacagtag aacgctttga
     5101 caaccgcttt atctatgtca accttggtag catcgaagcc caattgtcaa aacaagacca
     5161 aattcctgga gaagtttttg cttctcatga tcgtatcgaa gtttatgttt acaaggttga
     5221 agacaaccct cgtggtgtga acgtctttgt tagccgtagc catccagaaa tgatcaaacg
     5281 tttgatggag caagaaattc cagaagttta tgatggaact gttgaaatca tgagcgtggc
     5341 tcgtgaagca ggtgaccgta cgaaggttgc tgttcgtagc cacaatccaa acgtggatgc
     5401 tatcggtaca atcgttggac gtggtggtgc taatatcaag aagattacta gcaaattcca
     5461 cccagctcgt tacgatgcta aaaatgaccg catggtacca atcgaagaaa atatcgatgt
     5521 tatcgagtgg gtagcagatc cagctgaatt tatctacaat gccatcgctc ctgctgaggt
     5581 tgaccaagtt atctttgatg aaaacgacag caaacgtgcc ttggtggttg ttccagataa
     5641 caagctttct cttgccattg gtcgtcgtgg acaaaacgtg cgcttggcgg ctcacttgac
     5701 tggttaccgt atcgatatca agtctgctag cgaatttgaa gccatggaag acgctgcttc
     5761 agtagagttg gaagtagaaa acgatactgt agaagaataa aagctgctag aggagggaaa
     5821 gatgaaaaca agaaaaatcc ctttgcgcaa gtctgttgtg tctaacgaag tgattgataa
     5881 gcgtgatttg ctccgcattg tcaagaacaa ggaaggacaa gtctttattg atcctacggg
     5941 caaggccaat ggccgcggcg cttatatcaa actagacaat gcagaagccc tagaggcgaa
     6001 aaagaagaag gtctttaacc gcagctttag catggaagtg gaagaaagct tttatgacga
     6061 gttgatcgct tatgtggatc acaaagtgaa aagaagagag ttgggacttg aataagcaaa
     6121 agataagtaa tctcttgggg cttgctcagc gagcagggcg catcatatcg ggtgaagaat
     6181 tggtggtcaa ggccattcaa gacggcaagg ccaagttggt ctttctagct catgatgctg
     6241 gacccaatct ggccaagaag attcaagata aaagtcatta ttatcaagta gaaattgtaa
     6301 ccgtgttttc aacactggaa ttaagcatag cagtcgggaa atcgagaaag gttttggctg
     6361 taacagatgc tggatttaca aagaaaatga ggtctcttat ggaatagaag aggaggacat
     6421 gatttgtcta agaaaagatt gtacgaaatc gcaaaagaac ttggaaaaga aagtaaagaa
     6481 gttgtagcgc gtgcaaaaga gttgggcttg gatgtgaaaa gccactcatc aagtgtggaa
     6541 gaagctgtcg ctgcaaaaat tgctgccagc tttaagcctg cagctgctcc gaaagtagaa
     6601 gcaaaacctg cagcaccaaa agtaagtgca gaaaagaaaa ccgaaaaatc tgagccagct
     6661 aaaccagctg tagctaagga agaggcaaaa ccggctgagc cagttgctcc gaaaacagaa
     6721 aaagtagcag cgaaaccgca aagccgtaat ttcaaggctg agcgtgaagc ccgtgctaaa
     6781 gagcaggcag aacgacgcaa gcaaaataag ggcaataacc gtgaccaaca acaaaacgga
     6841 aaccgtcaga aaaacgatgg ccgtaatggt ggaaaacaag gtcaaagcaa ccgcgacaat
     6901 cgtcgcttta atgaccaagc taagaagcag caaggtcagc aaaaacgtag aaatgagcgc
     6961 cgtcagcaag aggacaaacg ttcaaatcaa gcggctccac gtattgactt taaagcccgt
     7021 gcagcagccc taaaagcaga gcaaaatgca gagtacgctc gttcaagtga ggaacgcttc
     7081 aagcagtatc aggctgctaa agaagccttg gctcaagcta acaaacgcaa ggaaccagag
     7141 gaaatctttg aagaagcggc taagttagct gaacaagcac agcaagttca agcagtggtt
     7201 gaagtcgtcc ctgagaaaaa agaacctgca gtggatacac gtcgtaaaaa acaagctcga
     7261 ccagacaaaa atcgtgacga ttatgatcat gaagaagatg gtcctagaaa acaacaaaag
     7321 aatcgaagta gtcaaaatca agtgagaaat caaaagaata gtaactggaa taacaacaaa
     7381 aagaacaaaa aaggcaataa caagaacaac cgtaatcaga ctccaaaacc tgttacggag
     7441 cgtaaattcc atgaattgcc aacagaattt gaatatacag atggtatgac cgttgcggaa
     7501 atcgcaaaac gtatcaaacg tgaaccagct gaaatcgtta agaaactttt catgatgggt
     7561 gtcatggcca cacaaaacca atccttagat ggggaaacaa ttgaactcct catggtggat
     7621 tacggtatcg aagccaaaca aaaggttgaa gtggataatg ctgacatcga acgtttcttt
     7681 gtcgaagatg gttatctcaa tgaagatgaa ttggttgagc gtccaccagt tgttactatc
     7741 atgggacatg ttgaccacgg taaaacaacc cttttggata ctcttcgtaa ctcacgtgtt
     7801 gcgacaggtg aagcaggtgg tattactcag catatcggtg cctaccaaat cgtggaaaat
     7861 ggtaagaaga ttaccttcct tgatacacca ggacacgcgg cctttacatc aatgcgtgcg
     7921 cgtggtgctt ctgttaccga tattacgatc ttggtcgtag cggcagatga cggggttatg
     7981 cctcagacta ttgaagccat caaccactca aaagcagcta acgttccaat catcgtagcc
     8041 attaacaaga ttgataaacc aggtgctaac ccagaacgcg ttatcggtga attggcagag
     8101 catggtgtga tgtcaaccgc ttggggtgga gattctgaat ttgttgaaat ctcagctaaa
     8161 ttcaaccaaa atatcgaaga attgttggaa acagtccttc ttgtagctga aatccaagaa
     8221 ctcaaggcag acccaacagt gcgtgcaatc ggtacggtta tcgaagcgcg cttggataaa
     8281 ggaaaaggtg cggtcgcaac ccttcttgta caacaaggta ccttgaatgt tcaagaccca
     8341 atcgttgtcg gaaatacctt cggtcgtgtc cgtgctatga ccaacgacct tggtcgtcgt
     8401 gttaaagttg ctggaccatc aacaccagtc tctatcacag gtttgaacga agcaccgatg
     8461 gcgggtgacc actttgccgt ttacgaggat gaaaaatctg cgcgtgcagc aggtgaagag
     8521 cgtgccaaac gtgccctcat gaaacaacgt caagctaccc aacgtgttag ccttgaaaac
     8581 ctctttgata cccttaaagc tggggaactc aaatctgtta atgttatcat caaggctgat
     8641 gtacaaggtt ctgttgaagc cctttctgcc tcacttcaaa agattgacgt ggaaggtgtc
     8701 aaagtgacta tcgtccactc agcggtcggt gctatcaacg aatcagacgt gacccttgcc
     8761 gaagcttcaa atgcctttat cgttggtttc aacgtacgcc ctacaccaca agctcgtcaa
     8821 caagcagaag ctgacgatgt ggaaatccgt cttcacagca ttatctacaa ggttatcgaa
     8881 gagatggaag aagctatgaa agggatgctt gatccagaat ttgaagaaaa agttattggt
     8941 gaagcggtta tccgtgaaac cttcaaggtg tctaaagtgg gaactatcgg tggatttatg
     9001 gttatcaacg gtaaggttgc ccgtgactct aaagtccgtg ttatccgtga tggtgtcgtt
     9061 atctatgatg gtgaactcgc aagcttgaaa cactataaag atgacgttaa ggaagttaca
     9121 aacggccgtg aaggtggatt gatgatcgac ggctacaatg atattaagat ggatgatgtg
     9181 attgaggcgt atgtcatgga agaaatcaag agataagatt ttttgctcct ttcttaggtg
     9241 gtgagggacg caagcaaacc gatggtttca ttgcttattt ttgagcctag ggtctcaaaa
     9301 atcccctgtg atgggactga taaatcagtt ccatcacttt caccacggcg aaagaagcag
     9361 atgacttcaa attgaacttc gtttcaattt aaactgaaaa tcaagaagtt taaaatagct
     9421 aggtctgctg gcctagcttt tggttcaaag tagagaaagg aatatcatgg caaatcattt
     9481 ccgtacagat cgtgtgggca tggaaatcaa gcgtgaagtc aatgagattt tgcaaaagaa
     9541 agttcgtgat ccacgtgtcc aaggtgtgac catcacagat gttcagatgt tgggtgactt
     9601 gtctgttgcc aaggtttatt acaccatttt gagtaacctt gcttcggata accaaaaagc
     9661 ccaaatcggg cttgaaaaag caactggtac catcaaacgt gaacttggtc gcaatttgaa
     9721 attgtacaaa atcccagatt tgaccttcgt caaagacgag tccatcgagt atggaaacaa
     9781 gattgacgag atgctacgca atctggataa gaactaaaga agaggggttg cccctctttt
     9841 ttggtggagg aaaataggtt gaatttgaaa tggaaaaata ttcttttaaa atatattgaa
     9901 actagaatag tacgcctcta cttctaaaac attgttagaa atcgatttga ctgtcctgat
     9961 cgatttgtcc tg
//
LOCUS       DAVKGC010000073         9921 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKGC010000073 DAVKGC010000000
VERSION     DAVKGC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.073"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            10..1011
                     /locus_tag="VW049_001484"
     CDS             10..1011
                     /locus_tag="VW049_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9432578.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1613..1693
                     /locus_tag="VW049_001485"
                     /pseudo
     CDS             1613..1693
                     /locus_tag="VW049_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            1999..2154
                     /locus_tag="VW049_001486"
     CDS             1999..2154
                     /locus_tag="VW049_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432579.1"
                     /translation="MEKLQFFLSQRACGCWKQAEKAVKLGSSETREENIILKGVPFIA
                     QPVTGDI"
     gene            2289..3650
                     /gene="trpE"
                     /locus_tag="VW049_001487"
     CDS             2289..3650
                     /gene="trpE"
                     /locus_tag="VW049_001487"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9432580.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3647..4213
                     /locus_tag="VW049_001488"
     CDS             3647..4213
                     /locus_tag="VW049_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9432581.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4224..5228
                     /gene="trpD"
                     /locus_tag="VW049_001489"
     CDS             4224..5228
                     /gene="trpD"
                     /locus_tag="VW049_001489"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9432582.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5225..5992
                     /gene="trpC"
                     /locus_tag="VW049_001490"
     CDS             5225..5992
                     /gene="trpC"
                     /locus_tag="VW049_001490"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9432583.1"
                     /translation="MSQEFLARILEQKVREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINHDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5979..6578
                     /locus_tag="VW049_001491"
     CDS             5979..6578
                     /locus_tag="VW049_001491"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9432584.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRAELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGEGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            6556..7779
                     /gene="trpB"
                     /locus_tag="VW049_001492"
     CDS             6556..7779
                     /gene="trpB"
                     /locus_tag="VW049_001492"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9432585.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLHQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGSPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7772..8548
                     /locus_tag="VW049_001493"
     CDS             7772..8548
                     /locus_tag="VW049_001493"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9432586.1"
                     /translation="MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(8520..8621)
                     /locus_tag="VW049_001494"
                     /pseudo
     CDS             complement(8520..8621)
                     /locus_tag="VW049_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            8850..9581
                     /locus_tag="VW049_001495"
     CDS             8850..9581
                     /locus_tag="VW049_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432587.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDENSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            9596..>9921
                     /locus_tag="VW049_001496"
     CDS             9596..>9921
                     /locus_tag="VW049_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9432588.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFV"
BASE COUNT         3039 a         1782 c         2318 g         2782 t
ORIGIN      
        1 aggactgaaa tgaaaaatat aaatgggaaa aaggtaacta tttatgatat tgcacgttta
       61 tcaggttttt ctccaaaaac agtttcacga gttattaatg gtggagtaaa tgttaaagaa
      121 gaaacttatc aagcgattca aaaagttata gaggaattgt cgtatattcc aaatgcgtat
      181 gcaaaaaatt taactaaaaa agaagctata aatatattga tttctgtaaa aaaaatcgac
      241 tcttttcctt taatttggtt tcatacgctg ttagataaag tgttacggac gtgtaaagaa
      301 tttggtgtta atgctatagt tgagtacttt ggagaggagg atacaattag taattcaatt
      361 atatcaagca caggtagcct agtagatggt gttattgttt tttatgaaag tgtagatgat
      421 atcagaattc agtatttaaa gaagaaccat atgccttttc ttgttttcgg tgaatctcaa
      481 acgtctggag tagtctatgt atctaataat aattttcaag ctacttatga tatgatgaaa
      541 gctgtaactg aagaaaaatt taaaaatatg tggttgctta tgggaggaga atcccatgtt
      601 aataaggatc gtgaaagagg tgtgcgttct tttctgaatg ataaaaatta ctttatggat
      661 ttaaaagtca tttatggctt atctacaatt gattcagttt attcttatgc aatgcagcac
      721 ttgactaccc aaaattatcc ggacattatt tttgtttcag gagatgaaaa ggttcagggc
      781 ttgattcgtg cgtgctatga gaaaggaatt cttattccag atgatatttc tatcattgga
      841 tttgataata ttcctatttc tcagtattat actccagctt tgtctacaat tgctcccaat
      901 tatgttaaat tggctaaaga aatgatagaa ggtgttttag caattattaa aggggaaagt
      961 gtcacatctg ttgaagtttc tcctaaattt gttaggagac agagtttcta gtattgttta
     1021 attcttgaaa atcctagtgg aaaagatgga gtgttctaaa ataagttctg tttagcctcc
     1081 attcagtttc tattgagttg gatccaattt ctaacgagtg ggacatgttc atgttcgatc
     1141 taaggaagac atggtcacat tcgaatgata ttttcaaaaa aatcgtaaaa aagtcttgac
     1201 aaagaaaaaa caaagtatta aaataagttc aacaaatcag aaaagtaact atttttgatc
     1261 ttcagggagc ctgtggtgat tgtgaatagg tggttggaag tagtgaaagt gggctgattt
     1321 tgaaaatgaa tttgaaacaa tgaaaattcg gtgcgcacac cttacagtgc agcttgttgt
     1381 taaacaaggc agagatgtaa agggatagtc cctttataat tgaggtggca ccgcgttacc
     1441 aacgccctca cacggaagta tattctgtgt gtgggctttt ttctatccgt cgtttggttt
     1501 atcttttatt agggccttaa gtcgctttga tgaacttgag ttctatctac agctccttgc
     1561 ctactactaa aagcaaacaa aaaggctgga ctaatacgaa aaaagctagt aatactcaat
     1621 gaaaatcaaa gagcaaacta ggaaactagc cgcaggctgc tcaaaacact gttttgaggt
     1681 tgtggataga actgacgaag tcagctcaaa acactgtttt gaggttgcag atagaactga
     1741 cgaagtcagc tcaaaacact gttttgaggt tgcagataga actgacgaag tcagctcaaa
     1801 acactgtttt gaggttgcag atagaactga cgaagtcagt aaccaatacc tacggcaagg
     1861 cgacgttgac gcggtttgaa gagattttcg aagagtataa aggtgtaccg agatgaacat
     1921 ctcttcatca atgtctctgg tcgtggaggc aaggacctag ctgcgattgc agactaccta
     1981 gaagctaaaa aataatagat ggaaaaatta cagttctttc tctcacagag agcttgtggt
     2041 tgctggaaac aagcagagaa agctgtaaag ttgggttcat ctgaaacgag agaagaaaac
     2101 atcattctca agggagtgcc ctttatcgca cagcctgtta caggagatat ctgagacagg
     2161 aatgagagat aggagaaatc ctataattga ggtggcaccg cgaatttcgt cctcacgcaa
     2221 gttattttgc gtggggattt ttcatacagt cgctacgaac tagaattaga actgaaaggg
     2281 aaattacaat ggaacgaatc attcatggag atgtcttatc accaatcttg gcttatatgc
     2341 gcttaaaggg gcaacacaag gttatcttag agagtattcc gagagacaag gaaacagctc
     2401 gtttttctat cctagcctat aatcctgttt ttgagattaa gtttgaaaat ggagttcttt
     2461 atcaaaatgg tcaagtgatt gatcgggatc ccttggattt cctttatgaa gtgattcata
     2521 agagtcagca ccattcagag ctaccttttg gtggtggagc cattggtttt gtgggttacg
     2581 atatgatttc gctttatgaa gaaattggtc aaatccctga ggatacaatt gggacgccag
     2641 acatgcattt ctttgtctat gagagctata tggtctttga ccacaagaag gagaagatac
     2701 atgtcataga ggatgcactt tatagtgagc gcagtcaaga ggccttggaa aaatccttga
     2761 accaagtgct tgaggaatta cgcattcctg ctccaaatga atttgaagat ttggatctat
     2821 ctccgttaga cttcaaaccg catatcgctc cacataagtt tgagggaatg gtagaaacag
     2881 ctcgtgactt gattcgtaac ggagatatgt tccaatgtgt actcagccag cgtttttcag
     2941 cagaagttac tggaaatcca tttgacttct acagaaatct ccgcgtgact aatccttcta
     3001 attaccttta tttctatgat tttggggatt atcaaatcat cggagctagt ccagaaagtt
     3061 tagtttctgt caaaaatggc atcgtgacaa ccaatccgat tgcagggacg cgaccaagag
     3121 gggctacgga tgaagaagac aagaccttgg cgactgacct cctctctgat gagaaggaaa
     3181 cagcagaaca tcgaatgttg gtagacttgg ggcgtaacga tattggccgc atctctgaaa
     3241 cggccagtgt ccaagttact aagtatatga aggtggagct cttccgctac gtcatgcatt
     3301 tgaccagcgt ggtcaagggg cgtttgcttc cagaactcac ggccatggat gccttgaaag
     3361 ctacacttcc tgctggaacc gtttctggag caccaaagat tcgggcgatg agacgcatct
     3421 atgaactgga aacggaaaaa cggggcgtat atgcaggagc aatcggctac ttgtctgcga
     3481 cgggtgatat ggatttggcc attgccatcc gaaccatgat tctcaaaaat caaagagcct
     3541 atgtgcaggc tggggcaggg attgtctacg actctatcgc ccaaaacgaa taccaagaaa
     3601 ccattaacaa agcaaaatct atgactagaa ttggagaact aagaccatga ttttattgat
     3661 tgacaactat gattctttta cctataactt ggcccaatac attgggaatt ttgcagaagt
     3721 tcaggttctg agaaatgatg attccaagct gtatgaagaa gctgaaaaag cagatggtct
     3781 ggtcttttct cctggtcctg gttggccagt tgatgctgga aagatggaag acatgattcg
     3841 tgattttgca ggcaagaagc cgattcttgg gatttgtttg ggccaccaag ccattgcaga
     3901 agtctttggt ggtaagctag gtttggctcc aaaagtcatg catgggaaac agagcaatat
     3961 caactttgaa gcgccatctg ttttgtatca aggtattgag gaaggacgtg cggtcatgcg
     4021 ttatcacagt attttgattg aggaaatgcc agaagacttt gaagtgacag ctcgttcgac
     4081 tgatgaccaa gccatcatgg ggattcaaca taaaaaccta ccgatttatg gcttccagta
     4141 ccatccagag agcattggaa cgccagacgg cttgtcttct attcggaatt ttatcgagaa
     4201 ggttgtaaag tgaggaaact aggatgaaag agattattga aaaactagca aaatttgaaa
     4261 atttatcagg tgtggaaatg acggatgtca ttgagcgtat cgtaactggg cgtgtaactg
     4321 aagcgcagat tgcttctctc ctcttagctc ttaagatgaa gggggaaaca cctgaagaac
     4381 gcacagccat tgcccaagtc atgagaggac atgcccagca tattccaact gagattcatg
     4441 atgccatgga caactgtggt acaggtgggg acaagtcttt cagttttaat atttccacaa
     4501 ctgcagcctt tgtcttggct ggtggcggta ttcacatggc caagcatggt aaccgctcga
     4561 tttcttctaa atctggttcc gcagatgtcc tcgaagcctt gggaatcaat cttgacctca
     4621 aaccagctga actaggtaag gtctttgata aaactggaat cgtctttctc ttcgctaaaa
     4681 atatgcaccc agctatgaaa tacatcatgc cagctcgttt ggaattggga attccaacga
     4741 ttatgaactt gactggtcca ctgattcatc caatggcttt agaaacacag cttcttggaa
     4801 ttagtcgtcc agaactccta gaaagtacag ctcaggtttt gaaaaatatg ggtcgcaaac
     4861 gtgccatcgt ggttgctgga ccagaagggt tggatgaagc tggcttgaac ggaacaacca
     4921 agattgcact tcttgaaaat ggcgaaatca gcttgtcaag ctttactcca gaggatttgg
     4981 gaatggaagg ctatgctatg gaagatattc gtggtgggaa tgctcaggaa aatgcagaaa
     5041 ttttgcttag cgttctgaaa aacgaagcaa gtccattctt ggaaacgaca gtcttgaatg
     5101 ctggtcttgg tttctatgct aatggtaaga ttgatagcat caaggaagga gttgccttgg
     5161 cccgtcaagt gattgctaga ggcaaggccc ttgaaaaact cagactgtta caggagtacc
     5221 aaaaatgagt caggaatttt tagcacgaat cttagagcag aaggtgcgtg aggtggagca
     5281 gatgaagctg gagcaaatcc agcctctgcg ccagacctat cgcttggcag agtttttgaa
     5341 gaatcatcag gaccgcttgc aggtaatcgc tgaggtcaag aaagctagcc ctagtttggg
     5401 agatatcaat cacgatgtgg atattgtgca acaggcccag acttatgaag aaaacggagc
     5461 agtgatgatt tcggtgttga cagatgaggt tttctttaaa gggcatttgg attatctacg
     5521 ggaaatttcc agtcaggtag agattccgac gctcaacaaa gactttatca tcgatgaaaa
     5581 gcaaatcatc cgcgctcgca atgcaggtgc gacagttatc ttgctcattg tggcagcctt
     5641 gtccgaagaa cgcctcaagg aactgtatga ctacgcgaca gagcttggtc tggaagtctt
     5701 agtggagact cacaatctag ctgaactaga ggtagcccac agacttggtg ctgagattat
     5761 cggggtcaac aaccgcaact tgactacctt tgaggtcgac ttgcagacca gtgtagattt
     5821 agccccttac tttgaggaag gtcgctatta catttctgaa tctgccattt tcacagggca
     5881 ggatgcggaa cgactagccc catactttaa cggaattttg gtagggacag ctcttatgca
     5941 ggcagaaaat gtggcccaga gaatcaagga gttgcagatt gacaaaggtt aaaatttgtg
     6001 gactatcgac caaagaagcg gtggaaacag ccgtttcagc aggagccgac tatatcggtt
     6061 ttgtctttgc acctagtaaa agacaggtga ctttagaaga ggcagctgag ttggcaaagc
     6121 ttattcctgc agatgtcaaa aaggttggtg tatttgtttc accaagtcgg gcagaactgc
     6181 tggaagcgat tgacaaagtt ggcttggact tggttcaagt tcacggtcag gtggcagatg
     6241 atttatttga gaatttgcct tgtgctagca ttcaggctgt gcaggtagat ggagagggtc
     6301 atgtgcccaa ttctcaggca gactatctac tctttgatgc ccctgtggct gggagtggcc
     6361 agccctttga ttggggtcaa ctggatacga ctggactagc acagcccttc tttatcgcag
     6421 gtggccttaa tgaagataat gtagtaaaag caattcaaca ctttactccc tatgcagtag
     6481 atgtatcaag cggagtggag acagatggac aaaaagatca tgaaaagatt agaagattta
     6541 tagagagggt aaagaatggc atatcaagaa ccaaataaag atggatttta cggaaaattt
     6601 ggcggacgtt ttgtcccaga aacattgatg acagcagttt tggagttgga gaaggcctac
     6661 cgtgaaagtc aggcagaccc aagtttccaa gaggaattaa accagctttt gcaccagtat
     6721 gtgggacgtg aaactcccct ttactacgca aaaaacttga cccagcatat cggcggagcc
     6781 aagatttatc tcaaacggga agaccttaac catacaggag cccacaagat taacaatgcc
     6841 ttaggacaag tttggcttgc caaacgcatg ggtaaaaaga aaattatcgc agaaactggt
     6901 gctggtcagc acggtgtggc aactgcaact gctgcggccc tctttaacat ggaatgtacc
     6961 atctacatgg gtgaggaaga tgtcaaacgc caagccctca atgtcttccg tatggagctt
     7021 ttgggagcta aggtcgaggc cgtgacagat ggttcgcgcg tgctcaagga tgcggtcaat
     7081 gcagcccttc gttcatgggt ggctaatatc gacgataccc actatatcct tggttctgcc
     7141 ttggggcctc atccatttcc agaaatcgtt cgtgacttcc aaagtgtcat cggtcgagag
     7201 gctaaacaac agtaccgtga cttgacaggt cgagatctgc cagatgccct agtagcctgt
     7261 gttggtggtg ggtcgaatgc tatcgggctc ttccatccct ttgtagaaga tgagtcagta
     7321 gctatgtatg gaactgaagc agcagggctt ggtgtggata cagagcacca cgctgctacc
     7381 ttgaccaagg gtagtccggg tgttcttcac ggttctctca tggatgtgct ccaagatgcc
     7441 catggtcaaa ttcttgaagc cttctctatc tcagcaggtt tggactatcc tggtatcggt
     7501 ccagagcatt ctcactacca cgatatcaaa cgtgccagct atgtccctgt gacagacgaa
     7561 gaagctttgg aaggatttca actcttgtct cgtgtggaag ggattatccc agccttggaa
     7621 tctagccatg ctatcgcttt tgcggtgaaa ttggccaaag aacttggacc agaaaagtct
     7681 atgattgtat gcctatcagg tcgtggggac aaggatgtgg ttcaagtcaa agaccgcttg
     7741 gaagcagatg cagcaaagaa gggagaagct catgcctaag acactaacag aaaaattgaa
     7801 tgctataaaa gcaactggaa aaggaatttt tgttccctat atcatggctg gtgaccacga
     7861 gaaaggtttg gacggtcttg ctgaaacaat tcacttttta gaagatttgg gtgtctctgc
     7921 aattgaagtg ggtattccct tttcagaccc tgttgcagat ggacctgtta tcgaagaagc
     7981 tggcttgcgc agtttagccc acgggacctc tacccaggct ttagttgaaa ccttgaaaac
     8041 cattgaaaca gagattccac tggtcatcat gacctacttc aaccccctct ttcagtacgg
     8101 tgtggagaac tttgtcaaag atctggctga tacagctgtg aagggcttga ttatcccaga
     8161 cctgcctcat gaacatgcca actttgtaga accatttttg gcagatacag atatcgcctt
     8221 gattccttta gtaagcttga ccacaggaat tgagcgtcag aaagagttga ttgaaggggc
     8281 ggagggattc atctatgccg ttgccatcaa tggagtgaca gggaaatcag gcaattaccg
     8341 tgcagatttg gacaagcact tggcgcaact tcatcaagtg gccgacatcc cagtcttgac
     8401 aggttttggt gtatctagtc aagccgatct ggaacgcttc aatgcggtgt cagatggtgt
     8461 tatcgtcggt tcaaaaatag taaaagctct ccaccaagga gagccgattc aggattttat
     8521 cagacaagca gtagcttacc aaaaataatc aaacaagtag caagtaagag gaaaggcacg
     8581 aaaggaagtc tttccttttt cttttgcagg agaaaggtca gttttatagt gaattgaatc
     8641 tggaatatta cactacggtt cctaaaatat tttcagaaag cgatttgaat ttcccaataa
     8701 atttgttcag tttttaattg attttactat agaagatata attttctaaa ttactctgac
     8761 acagaacttt aagaaaaaga taattattgt agtaatttct ttaagaaaat ggtataattc
     8821 attaaaaagt atttggagga ggaagaatta tgttgaatca ggatctcttt gattcgcttg
     8881 aggcacaaaa aattgtagat actttgatga aagggcaaaa agattatgta gatgaacgtc
     8941 tagaaaaaag agagacaatg atagtgtcga atggttatgc atggacgcga cctaatcata
     9001 ttgatactgc atttgcatca gcagatttgt ttgagtataa attacaatta gcaggacaga
     9061 cttggggata tttagaattt gaaacaaata cagaaaaata tgggaaagta ttgttaatta
     9121 taaagggtaa gaagcgactt acgaaccaat ttcctttggt acaaaaaaat aagagtggct
     9181 acttatttga atatgctcag atgaatacac tttatcttaa tcaacattct tcctacaaaa
     9241 atgatgaaaa tagtcattcc tttccaattc agatggagtt agtttctgat gaaatgattc
     9301 aagaaattga acaagctact aaaaattcga atatcgaaaa atttatgatt ttaacttatg
     9361 aggcggactc agaaaacaat attatatctg tagatgttgt tatgcctgat gcacgaactg
     9421 gtcagttaca cttgattcaa gatttgtctg agtatattca atcaagttcg taccatttcg
     9481 aagaagctaa atatcaagat attcctaatt tttcagaatt atcggaaaca gaagattttg
     9541 aaattattcc aagaatagaa aaacaagaag gtcaaaagta gtaaggagta tgtaaatgtt
     9601 taatggtcgg gtattgaaag aattacggct gttaaatggt ttaagtagag cagaattagc
     9661 tcagagaatt aatttaacgg aacaagccat ttggcagttt gagtccaacg aaacgaaacc
     9721 taaattatca accaaaatgc atttggccaa ccaatttcat gttgatttaa cttattttga
     9781 acaggaagaa gagagcattc gatttgattc ttctgtaatt gcctttagaa atgcagacct
     9841 agcaacacgg aaaacaatag atattcaaac tatgtattta cataaggtag atagtttgat
     9901 tgattatttt gaaagttttg t
//
LOCUS       DAVKGC010000074         9920 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKGC010000074 DAVKGC010000000
VERSION     DAVKGC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.074"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            480..1523
                     /locus_tag="VW049_001497"
     CDS             480..1523
                     /locus_tag="VW049_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9432589.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1690..3777
                     /gene="topA"
                     /locus_tag="VW049_001498"
     CDS             1690..3777
                     /gene="topA"
                     /locus_tag="VW049_001498"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9432590.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3998..4357
                     /locus_tag="VW049_001499"
     CDS             3998..4357
                     /locus_tag="VW049_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9432591.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4450..5082
                     /locus_tag="VW049_001500"
     CDS             4450..5082
                     /locus_tag="VW049_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9432592.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILKYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            5126..5782
                     /locus_tag="VW049_001501"
     CDS             5126..5782
                     /locus_tag="VW049_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9432593.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5801..6433
                     /locus_tag="VW049_001502"
     CDS             5801..6433
                     /locus_tag="VW049_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU9432594.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6461..6895
                     /locus_tag="VW049_001503"
     CDS             6461..6895
                     /locus_tag="VW049_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU9432595.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6907..7944
                     /gene="leuB"
                     /locus_tag="VW049_001504"
     CDS             6907..7944
                     /gene="leuB"
                     /locus_tag="VW049_001504"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU9432596.1"
                     /translation="MAKKIVALAGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAVVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDYILEERNARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7941..8210
                     /locus_tag="VW049_001505"
     CDS             7941..8210
                     /locus_tag="VW049_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9432597.1"
                     /translation="MKLDEKIALVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALICGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8214..8573
                     /locus_tag="VW049_001506"
     CDS             8214..8573
                     /locus_tag="VW049_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9432598.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPIDQATVDLEQQKIISPVEEFTFEIDSEWKHKLLNTLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8735..9305
                     /locus_tag="VW049_001507"
                     /pseudo
     CDS             8735..9305
                     /locus_tag="VW049_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019771122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9399..9464
                     /locus_tag="VW049_001508"
                     /pseudo
     CDS             9399..9464
                     /locus_tag="VW049_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9627..>9920
                     /locus_tag="VW049_001509"
     CDS             9627..>9920
                     /locus_tag="VW049_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432599.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNK"
BASE COUNT         3107 a         1707 c         2200 g         2906 t
ORIGIN      
        1 actgttatag attcaataca caataattta gtgtaattga gaaaggagaa atgattgtga
       61 ttgatgttgg ctaggttatg ttcaatgatt cctaccgtct caaatcttgt cagtaaggaa
      121 aaataaattc ttcaaaagta gagattacaa ggcttgttta agaaagaatt caaagacctt
      181 gacaaataaa aataaaatgg ttattataaa aaatggtctg aaatagatga tgatactttt
      241 cgaaaatctt tttacgtcag ctcagctttg ccttgctgtg ttttgagcaa gctacggtta
      301 gcttccgagt ttgattttca tttactagaa atgaaactga tgagagatat cagtagacat
      361 ctgagttagg atattatgga aaatgataaa aagagctcgt gagattggca tatcagacta
      421 ctaaagtatt gagtttgtta ggattttagc gactagttag ctgggaaagg aagatatttg
      481 tgacaaataa taaactgtat tcgttgatag aatttagaaa taaaatatat gaagaattag
      541 aactttccag aagtgattta gcgattttac tatgtgccat gcttatcgcc tctatcggat
      601 taaatatgga ttcgactccc gtgattattg gagccatgtt aatctctcct ttgatgacac
      661 ctattctggg agtggggctc tctctagcta tatttgattt taaattgtta agaaaatctt
      721 ttaaaatatt agctattcaa attcttgcca gtctaatagc ttcaacactt tatttttatc
      781 tttctcccat ttcgtatgct agttcggaga ttgttgctag aacctctccg actatttggg
      841 atgttctcat tgcttttgta ggagggatag caggtattat tggtgctagg aaaaaagaga
      901 ccaataatat tgttcctggt gttgctattg caaccgcctt gatgcctcct ctttgtacag
      961 taggttatgc tattgcttct gctaatctaa aatttatcat aggctcctct tacctattcc
     1021 tcatcaattg tagctttatt gtcattgcga cttatatagg tgttaggttg atgatggtta
     1081 agaaacatta ttttaaagat aatgaagaag actctaaaat gcgtaggatt ttgcttctag
     1141 ttgctgtttt gctgatgatt ccgagtttca tctctgcaac gactttagtg agagaaacgt
     1201 tgaaaaaaga gtcccttaag aaatttatat cagagcagtt tcaggggtat aatattttga
     1261 aaaaaaccta ttctaaaaag actcataccc taaagctaac catttcagga aattatttga
     1321 cagaagaaga actcgatatg atttcaagta agagaggtga ctatggttta agtgatgttt
     1381 ctgttcaagt ttcacaattg tctgattcag aacaacttag taaggaagag ctggtggagt
     1441 atttcttcca gtatatcaag gataaggaag caaaagaaaa ggagaaagct aataaatttg
     1501 atacagagtc tgaggagcaa taatttcttg agaatagctg gtttctctcg tgagtcttct
     1561 atgtatatca aaggaagact gaggttttaa gtataaaact tttcttctat tatagtagct
     1621 aagtcttgac agggttcaaa aaatggttta cactttataa agtttattac tttgaaaagg
     1681 tgtgatactg tggctacggc aacaaaaaag aaaaaatcaa cagttaaaaa aaatctagtc
     1741 atcgtggagt cgcctgctaa ggccaagacg attgaaaaat atctaggcag aaactacaag
     1801 gttttagcca gtgtcgggca tatccgtgat ttgaagaaat ccagtatgtc cgtcgatatt
     1861 gaaaataatt atgaaccgca atatatcaat atccgaggaa aaggccctct tatcaatgac
     1921 ttgaaaaaag aagctaaaaa agctaataaa gtttttctcg cgagtgaccc ggaccgtgaa
     1981 ggagaagcga tttcttggca tttggcccat attctcaact tggatgaaaa tgatgccaac
     2041 cgtgtggtct tcaatgaaat caccaaggat gcagtcaaaa atgcttttaa agaacctcgt
     2101 aagatcgata tggacttggt cgatgcccaa caagctcgtc ggatcttgga tcgcttggta
     2161 gggtattcga tttcgcctat tttgtggaag aaggtcaaga agggcttgtc agcaggtcgc
     2221 gttcagtcca ttgcccttaa actcatcatt gaccgtgaaa atgaaatcaa tgccttccag
     2281 ccagaagaat actggacagt tgatgctgtc tttaaaaagg gaaccaaaca atttcatgct
     2341 tccttctatg gagtagatgg taaaaagatg aaactgacca gcaatgacga agtcaaggaa
     2401 gtcttgtctc gtctgacgag taaagacttt tcagtagatc aggtggataa gaaagagcgc
     2461 aagcgcaatg ctcctttacc ctataccact tcatctatgc agatggatgc tgccaataaa
     2521 atcaatttcc gtactcgaaa aaccatgatg gttgcccaac agctctatga aggaattaat
     2581 atcggttctg gtgttcaagg tttgattacc tatatgcgta ccgattcgac tcgtatcagt
     2641 cctgtagcgc aaaatgaggc ggcaagcttc attacggatc gttttggtag taagtattct
     2701 aagcacggta gcaaggtcaa aaatgcatca ggtgctcagg atgcccatga ggctattcgt
     2761 ccgtcaagtg tctttaatac accagaaagc atcgctaagt atctggacaa ggatcagctc
     2821 aagctatata cccttatctg gaatcgtttt gtggctagcc agatgacagc ggccgttttt
     2881 gataccatgg ctgttaaatt gtctcaaaat ggagttcaat ttgctgccaa tggtagtcag
     2941 gttaagtttg atggttatct tgccatttat aatgattctg acaagaataa gatgttacct
     3001 gacatggttg ttggagatgt ggtcaaacag gtcaatagca aaccagagca acatttcacc
     3061 caaccgcctg cccgttattc tgaagcaaca ctgattaaaa ccttagagga aaatggggtt
     3121 ggacgtccat caacctatgc gccaaccatt gaaaccattc agaaacgtta ttatgttcgc
     3181 ctggcagcca aacgttttga accgacagag ttgggagaaa ttgtcaataa gctcatcgtt
     3241 gaatatttcc cagatatcgt aaacgtgacc ttcacagctg aaatggaagg taaactggat
     3301 gatgtcgaag ttggaaaaga gcagtggcga cgggtcattg atgcctttta caaaccattc
     3361 tctaaagaag ttgccaaggc tgaagaagaa atggaaaaaa tccagattaa ggatgaacca
     3421 gctggatttg actgtgaagt gtgtggcagt ccaatggtca ttaaacttgg tcgttttggt
     3481 aaattctacg cttgtagcaa tttcccagat tgccgtcata cccaagcaat cgtgaaagag
     3541 attggtgttg agtgtccaag ctgtcatcag ggacaaatta ttgagcgaaa aaccaagcgt
     3601 aatcgcctct tctatggttg caatcgctat ccagaatgtg aatttacctc ttgggacaag
     3661 cctgttggtc gtgactgtcc aaaatgtggc aacttcctca tggagaaaaa agtccgtggt
     3721 ggtggcaagc aggttgtttg tagcaaaggc gactacgagg aagaaaagat taaataagag
     3781 gaggggcccg aaagtaaatt tcaggctcct tttggttaga acttgacaaa atacatcatt
     3841 ttatgcgaaa ctagaagaag attattttat actcaatgag aatcaaagag caaattagaa
     3901 agctagtcgc aggttgctca aagcactact ttgaggttga agatggaagc tgacctggtt
     3961 tgaagagatt ttcgaagagt attaactagg agaagttatg cgtcttatct atctgataat
     4021 tggtttttta tcactggcct tggctattgt tggggttgtt ttacccttgt tgccgacaac
     4081 acctttcctt ttgttgtcta ttgcttgttt ctccagaagt tccaagcgat tcgaagattg
     4141 gctttatcat accaagctct atcaagcata tgtagctgat tttcgtgaga ccaagtctat
     4201 tgcgcgtgaa cgaaagaaaa aaatcatcgt ctctatctac gtcttgatgg gaatttctat
     4261 ttattttgca cctcttttac cagtcaaaat cggtctgggt gctttgacca tctttattac
     4321 ttattatctc ttcaaggtca ttccagacaa agaatagtta aaacagtagt tatttgcctt
     4381 gataaaattg aaagcatatt cataacaata tgatataata aaattgaagt aatattcaag
     4441 gagaatcaaa tgatttacga attttgtgct gaaaatgtga ctttacttga aaaagcgatg
     4501 caggctggag ctcgtcggat tgaactctgt gataatctag cagttggtgg gacaacaccc
     4561 agctatggag tgactaaggc agcggttgaa ctggcagcta actacgatac aaccatcatg
     4621 accatgattc ggccacgtgg tggtgacttt gtctacaatg acctagaaat tgctatcatg
     4681 ctagaagaca ttcgtttgac tgctcaggct ggaagtcaag gggttgtatt tggagcttta
     4741 actgctgata aaaagttgga taagcctaat ctggaaaagt taattgctgc atcaaaagga
     4801 atggaaattg tctttcacat ggcctttgat gaactaagtg atgaagatca accggaagct
     4861 attgactggc tcagtcaagc cggtgtcact cgtatcctaa ctcgtgctgg tgtgtctggc
     4921 gactccttag aaaaacgttt tgttcactat cacagaattt tgaagtacgc taaaggtaaa
     4981 attgaaattc taccaggtgg ggggattgac cttgaaaacc gtcaaacctt tatcgaccag
     5041 gtgggggtaa cacaattgca tggtactaag gttgtttttt aaaaaataga aaggaactgc
     5101 tagctttggg tagcagtttt cacttatgtt tgaaattttt aaatcctatc aatttaatca
     5161 agaaaaggct catgattatg gttttataga aaatagcgaa gtctggacat atagttgcca
     5221 gattttgcaa ggtgactttg tcatgactgt gtccatcact gctgataatg tgaactttca
     5281 agtctttgac caagagactg gtgacctcta tcctcacgtt tatatggaaa gcatgagggg
     5341 aagttttgtc ggaaatgtcc gtgaggcttg tctggagatt ctttaccaga ttcggaaggc
     5401 ttgttttgat gtgcaagatt ttatctgtca tcagactaag cgtatcatga ctcaagttca
     5461 ggaaaagtat ggaaaccagt tggagtatct gtgggaaaaa tcgcctgata cagctgtatt
     5521 gcgccatgaa ggcaatcaaa agtggtatgc cgtcttgatg aaaatctctt ggaataagct
     5581 ggaaaagggc agagaaggac aagtggaagc agtcaacctc aagcatgacc aagtagctaa
     5641 tttgctttca caaaagggga tttatccagc cttccatatg agcaagcgct actggattag
     5701 tgtgtccctt gatgatactt tatcagatga agaagtactg gaattgatag aaaaaagttg
     5761 gaacttaacc tctaaaaaat gaaatatttt aataattttc atgaactttc aattagctaa
     5821 atattcttta ctgaagaaat tttcagaaaa tataggattt accacaccag aggaatgtgg
     5881 tgccatcttc aaatacctga ttgagaatgt caagacggat cgtcagatca tctattcgcc
     5941 tcactgtcat gatgacctcg gaatggcagt ggcaaatagc cttgctgctg tcaagaatgg
     6001 tgcaggacgt gttgaaggga ctatcaatgg tattagggag cgagctgaaa atgctgcttt
     6061 ggaagaaatt gcagtggctc tcaatattcg ccaagattac taccaagtag aaaccagtat
     6121 tgtcctaaat gagaccatca atacgtcaga aatggtttct cgcttctctg gtattccagt
     6181 tcctaaaaac aaagccgtcg ttggtggcaa taccttctcc cacgaatctg gtattcacca
     6241 agatggagtc cttaaaaatc ctctcactta tgagatcatc acacctgaat tggttggtgt
     6301 taagattccg cttggaaaat tatctggtcg ccatgctttt gttgagaaac tgagagaatt
     6361 ggccctagat tttacagaag aggatatcaa accactcttt gctaagttca aggcactggc
     6421 cgataagaag taagaaatca cagatgcaga tactcgagct ttggtagctg gaaccatggt
     6481 tgaaaatcca gaaggcttcc actttgatga tttacaactt caaactcatg cagataatga
     6541 cattgaagcg ctcgttagcc tagccaatat ggatggtgag aaagtcgaat ttaatgcgac
     6601 agggcaaggt tccgttgaag caatctttaa cgctatcgat aagttcttta accaatctgt
     6661 tcgtttggtg tcctacacta tcaatgcggt aacagatgga atcgatgccc aggatcgggt
     6721 tttggtcact gttgaaaaca gagatacaga aaccatcttt aatgcagcag ggcttgattt
     6781 tgatgtgttg aaggcttctg ctattgtcta tataaacgct aatacctttg ttcaaaaaga
     6841 gaatgcaggt gagatgggac gcagtgtttc ttaccacgat atgcctagtg tgtaaaggag
     6901 aaggctatgg caaagaaaat agtagctcta gcaggagacg gaattggccc agaaatcatg
     6961 gaggctggtt tagaagttct ggaggctcta gctgaaaaaa caggttttga ctatgagatt
     7021 gacagacgac cgttcggagg tgcagatatt gatgcagcag ggcctccctt acctgatgaa
     7081 acccttaagg caagtaggga agcagatgct atcctactag cagctatcgg tagtcctcag
     7141 tatgatggag cagtggttcg ccctgaacaa ggcctgatgg ctctccgtaa ggaactcaat
     7201 ctttacgcta atattcgtcc tgtaaaaatc tttgacagtc tcaagcattt gtcaccactc
     7261 aaactggaac gaattgctgg tgtagacttt gtcgtggtgc gtgaattgac aggcgggatt
     7321 tactttggag attatattct tgaagagcgc aatgcgcgtg atatcaacga ctatagctat
     7381 gaggaagtgg agcggattat tcgcaaagcc tttgaaattg caagaaatcg cagaaaaatc
     7441 gttactagta tcgataagca aaatgttcta gcgacctcaa aactctggcg gaaagtagct
     7501 gaggaagtcg cacaggattt cccagatgta accttggaac accagctggt agactcagct
     7561 gctatgctta tgattaccaa tcctgctaag tttgatgtta ttgtaacgga gaatcttttt
     7621 ggagatattt tatctgatga atcaagcgtc ttatctggta cacttgaggt tatgccatca
     7681 gccagtcatt ctgaaaatgg accaagtctc tatgaaccta ttcacggttc agcacctgat
     7741 attgcaggtc aaggaattgc caatcctact tccatgattt tatcagttgc catgatgttg
     7801 agagatagtt tcggacgtta tgaggatgca gagcgtatca aacgtgctgt tgagacaagt
     7861 ctggcggcag gaattttaac gagagatata ggaggtcagg cttcaacaaa ggaaatgacg
     7921 gaagctatta ttgcaaggtt atgaagttag acgaaaaaat tgctctagtc cttttgattt
     7981 ggaatgtcat cattttcttg atttatggca ttgacaaatt caaggcaagg agaagagctt
     8041 ggcgcatcca agagaaaatc ttactcattt tagcccttat ttgtggtggt tttggggcct
     8101 ggttagctgg aatcactttt caccacaaga ctcgaaaatg gtactttaaa acagtttggt
     8161 ttcttgggat ggtgaccaca ctagtagcct tatattttat ttggaggtaa tggatggcag
     8221 ggtcttcgag ggaacacgct gcttgggttc tagcggacta tggttttaag gtcgtgattg
     8281 caggatcttt cggtgacatt cattacaata atgaactcaa taatggcctg ttgccaatcg
     8341 ttcagcctag agaggttaga gagaaattag cccagctaaa accaatcgac caggcaactg
     8401 tggacttgga acaacaaaaa atcatctcac cagttgaaga attcaccttc gagatagata
     8461 gcgagtggaa acataaactc ctaaatactt tggatgatat cggtattacc ttgcagtatg
     8521 aagagttgat tgctgcttat gaaaaacaac gaccagccta ctggcaggat tagaaaaaat
     8581 agaaaaggaa atatagtaaa ctgaaataag atgtaaacaa atgaattaga gcttaacatc
     8641 catttccagc aattttttag aaactacagt ggactattct ggattcaaca cattataaaa
     8701 ttatgacaaa acacattcac aagaaggcta cgacatttta aaaggtgagg gcggatgtat
     8761 cgtttgccct actaaagttg gttacattat catgaccagt gacaaggcag gacttgagcg
     8821 taagttcgca gccaaagaac gtaagcgtaa caaaccaggt gttgttctct gcggtagcat
     8881 ggatgaactt cgcgctttag cgcaactcaa cccagaaatt gaagcattct actaaaaaca
     8941 ttgggatgaa gatattcttc ttggttgtat ccttccttgg aaaccagaag cctctgaaaa
     9001 actcaaagca tacggggatg gccgtgaaga acttatgact gatgtacgtg gtactagctg
     9061 ttttgttatc aagtttggaa aagcaggtga acaattggct gccaagcttt gggaagaagg
     9121 taaaatggtc tacgcctcat ctgcttcaat gacaaaacga ttgaaactcg ctatgagagc
     9181 aaggtgtaat ggtgtctatg gtcgataagg acggcaaact catcccagaa caaggaggag
     9241 cacgttcaac ttcaccagca ccagttgtga tccgtaaagg gcttgacatt gatatagtaa
     9301 aatgaagtaa gagcaggaca aattaatcag gacagtcaaa tcgatttcta acaatgtttt
     9361 agaagtagag gtgtactatt ctagtttcaa tccactataa aatcatgatg cacctgtcag
     9421 atacttttaa ctcatgggac taccgtcagg ttgagtatta ttaggataga gaagaagtct
     9481 agtgttaagg acattacagc tcataatact aggctttttt atagaaattc ttttcttttt
     9541 ctataggata tggtattcta tgtagaaaat atatgttaat aagtaatgcc aatatttaaa
     9601 catcattagt aaaaggagtt agattgatga ataaaagaaa agttagttta gaagattttt
     9661 ataaatggta tagtctaaat aaagaagagt tattaaataa ggcaactgtt ggtgaaaagt
     9721 ttaatgataa attaaaagaa gagtttctcc aggaatggcc tttggatagg attttaacaa
     9781 tgtcaatcga tgaatatgta ataggaaagg gacagcaaaa taagtcttta tgctacgctc
     9841 ttgagaaggg aaaatacaaa aatctatttc ttggaatttc tggtggctca gcttcaaaat
     9901 ttggtattta ttggaataaa
//
LOCUS       DAVKGC010000075         9786 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKGC010000075 DAVKGC010000000
VERSION     DAVKGC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.075"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            68..931
                     /locus_tag="VW049_001510"
     CDS             68..931
                     /locus_tag="VW049_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9432600.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1314..1934
                     /locus_tag="VW049_001511"
     CDS             1314..1934
                     /locus_tag="VW049_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432601.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            2020..2463
                     /gene="tsaE"
                     /locus_tag="VW049_001512"
     CDS             2020..2463
                     /gene="tsaE"
                     /locus_tag="VW049_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9432602.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2453..2971
                     /locus_tag="VW049_001513"
     CDS             2453..2971
                     /locus_tag="VW049_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9432603.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2979..3995
                     /locus_tag="VW049_001514"
     CDS             2979..3995
                     /locus_tag="VW049_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9432604.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4080..5336
                     /locus_tag="VW049_001515"
     CDS             4080..5336
                     /locus_tag="VW049_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9432605.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5391..6557
                     /gene="recA"
                     /locus_tag="VW049_001516"
     CDS             5391..6557
                     /gene="recA"
                     /locus_tag="VW049_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9432606.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            6863..8233
                     /locus_tag="VW049_001517"
     CDS             6863..8233
                     /locus_tag="VW049_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9432607.1"
                     /translation="MNKKRTVDLIHGPILLSLLSFAFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVFFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8609..9565
                     /gene="lytA"
                     /locus_tag="VW049_001518"
     CDS             8609..9565
                     /gene="lytA"
                     /locus_tag="VW049_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9432608.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
BASE COUNT         2993 a         1667 c         2174 g         2952 t
ORIGIN      
        1 taacttgcta ttttgaacta aaaattttat aatgcaatta agcaaatagg aaggtttatt
       61 atctttaatg aatacactcg cagagaaatt cagattgaaa agaaaagagt tgagactctc
      121 ccaacaaact cttgcagaag gaatttgtga acaaagtcag attagtaaaa ttgagagagg
      181 gcatttcatt ccctccgcag accttttgtt caaactctct caacgacttg aagtaccatt
      241 agattatttt tttaatgaac aaattgaaat taaatctaac ctctctaatt tcaagcaatt
      301 gtctgctcga ctattagacg atagaaatta tgacgatttg gaatatattt atagaataga
      361 gattgaacga agtacttttc taacactaga agaccgaact taccttgaat ggattaaagc
      421 tattattgac ttctatcaat atgacagtaa gtgtgaggca atttcttcat tggaaaatat
      481 attattaaaa gtctcttcaa atactctgat ttatttaaag gcgttgaata ctctatctaa
      541 tttctattcc ttagtgggtc gtgaacaaga atatgaggca aactactctc atttaataga
      601 gttgtatcag acaaaaaatt tggaccatca agagttttta tttggctaca tcagagttcg
      661 ttacaactac gctcactacc tagtatcaaa ggaaaaatat aatgaagcca tccaagaagc
      721 tcttgaaacg attgaactct gtaaacaaag acagacaagc taccaattgg ctcctctact
      781 tattcttgta ggaaatgctg gagccaaatt tctagacaga gaacaagtca aaaattatta
      841 tatagaagca agagagttat gtaagattta taacaatcct ttaatgttga tgaagataga
      901 aaattatttg aaggaactag atactgttta gttaatcttg acattatagt ttttctgaaa
      961 cgttaaataa cctgtaaggc tgatagttag attaatacta tcagccttat aattatgtga
     1021 ctctgagtcc gtctcgctcc gtgaggtgcg agacaaaaca accacccgtt tgacggattt
     1081 tcatgatttc aaatttatat tcttcgtaaa tctcttcaaa ccacgtcagc tctatctgca
     1141 atctcaaaac agtgttttga gcaacctgtg gctagctacc tagtttgctc tttgattttc
     1201 attgagtatt agtctctagt gtttgtttag cgtcttaaag agacatttga gaagattaga
     1261 aacagttctc tcttttctcc tagtataatg attggtagga agggagagag aagatgaaat
     1321 caatgagaat cttatttttg ttagctttaa ttcaaatcag tttgagtagc tgtttcctat
     1381 ggaaggaatg catcttgtcc tttaaacaaa gtacagcttt tttcatcgga agcatggttt
     1441 tcgtttcagg aatctgtgct ggagtaaatt atctttatac tcgtaagcaa gaagtccata
     1501 gtgtcctagc cagtaagaag tcggtgaagc ttttttacag tatgttactc ttaattaatt
     1561 tgttaggagc tgttcttgtt ttgtcagata acttgttcat caaaaatacg ctgcagcaag
     1621 aattagttga ctttttattg ccatccttct ttttcctatt tgggctagat ttgctgattt
     1681 ttttaccctt gaaaaaatac gtgcgcgatt ttcttgctat gctggacaga aaaaagacag
     1741 tgttggtgac tattttagca acacttcttt tcttaagaaa tccaatgacc attgtctcac
     1801 ttctgattta tattggactg ggcttgtttt ttgcagccta tcttgtccca aattcggtta
     1861 agaaggaagt ttccttttat ggtcatattt tccgagatct tgtattggtc attgttacgc
     1921 tcattttctt ttagagaaag cctgtttttt atggatgtta tgatgataag tttagtagga
     1981 aaatagacac aaaagaaaag tttttggtat aataagagta tgtacacaaa aaatgaagaa
     2041 gagttgcaag ccttagggga gcgtttgggc catctattag caaagaatga tgttttaatc
     2101 ttaactggag aactgggtgc aggtaaaacg acctttacta aaggacttgc aaaaggatta
     2161 cagatttctc aaatgattaa aagtcccacc tatactatcg tgagagagta tgaaggtcga
     2221 cttccacttt atcacctaga tgtttatcgt attgaaggag atgctgattc tatcgacttg
     2281 gatgagttta tctttggtgg cggcgtgact gttattgagt ggggaaatct cttaggagat
     2341 gccttgccag atgcttattt agaattggaa attctaaaag aagcagatgg acgccgttta
     2401 aattttcagg caaagggttt gcgtgctgag aaattgttag aggagcttca atatggagta
     2461 tgaattgctc attagggaag cagagcccaa agatgcagct gaattagtgg cctttttaaa
     2521 tcgtgtgagt ttggagacag actttaccag cctagacgga gatggtattc tcttgactag
     2581 tgaggagatg gaaatattcc tcaacaagca agctagttcg gacaatcaga taaccttact
     2641 tgcattctta aatggcaaaa ttgctggtat tgtaaatatt acagctgatc agcgtaagag
     2701 agtccgtcat attggagatc tcttcattgt gattggaaaa agatattgga ataatggctt
     2761 gggaagtttg ttgctagaag aagcgataga gtgggcacaa gcaagtggca ttctgcgtcg
     2821 tctccaactg actgtccaaa ctcgtaatca agcagcagtc catctttatc aaaagcatgg
     2881 ctttgtcatt gaaggtagcc aagagcgtgg ggcatatata gaagaaggga aatttatcga
     2941 tgtttacttg atgggtaaac tgataggtta gtagaaatat ggttaaaaaa attattggaa
     3001 tggtgctagc tttactttct gtaactgtag taggagtagg tgtttttgct tatactattt
     3061 atcaacaagg gacagaaacc ttagctaaaa cctataaaaa aatcggcgaa gaaaccaagg
     3121 ttattgaagc gactgaacct ctaaccattc tgttaatggg agtggacacc ggaaatgctg
     3181 aacgaactga aacttgggtc ggtagaagtg atagcatgat cttgatgaca gtgaatccta
     3241 aaacgaaaaa aacaacaatg atgagtttag agcgggatat tctgacgcgc attgaatcag
     3301 ggaatggtca ggctcatgaa gcgaaactga actcagcata tgcagatggt ggagcagagc
     3361 ttgctataga aaccattcaa aaaatgatga atatccatat tgatcgctat gtgatggtca
     3421 atatgagagg gttgcaaaaa ctagtggatg cagtaggagg tattacagtc aataatatcc
     3481 taggtttccc aatttctatc agtgaccaag aagaatttaa tactatttct atcggtgttg
     3541 gggagcaaca tattggggga gaagaagccc tagtctatgc acgaatgcgt taccaagatc
     3601 ctgaggggga ttatggtcgt caaaaacgtc aacgtgaagt tattcaaaaa gtcatggaaa
     3661 aagctctcag tttaaatagc gttggtcatt atcaagagat tctaaaagct ttgagtgaca
     3721 atatgcagac caatattgat ttgtctgcaa aaagtatccc taacttgcta ggctataaag
     3781 attcatttaa aaccattgaa actcagcagt tgcagggtga aggagagata cttcaaggtg
     3841 tttcttacca gattgtttcg agagcacata tgttggaaat gcaaaatata ctccgacgtt
     3901 ctttgggaca agaagaagtt actcagcttg aaaccaatgc ggttttattt gaagatttat
     3961 ttggcagagc acctgttggt gatgaagata attaactttt gatacaaata aaaaaatcaa
     4021 tcgtaggaaa attcctgcga ttttttcaaa aaaatacgaa tagataggta ggaggaaaca
     4081 tgaaagcaga aatcattgct gttggaacag agattttgac aggacagatt gtcaacacca
     4141 atgcccagtt tttgtcggaa aaactagctg agattggggt agatgtatat tttcagacgg
     4201 ctgtaggaga caatgaagtt cgtctcttgt ctttgcttga gattgccagt caacgtagca
     4261 gtctggtgat tttgactggc ggtttggggc caactgagga cgacctaacc aaacaaaccc
     4321 tagctaaatt tttagggaaa gcattagtct ttgatcctca ggctcaggag aagttggata
     4381 tcttttttgc cctgcgacca gactatgccc gaacaccgaa taacgaaaga caagctcaaa
     4441 ttgtagaagg agcgattcca ctgccaaacg aaacaggact ggctgttgga ggaaaattag
     4501 aagtagacgg agtgacctat gtcgtccttc caggtccgcc aagtgaattg aaacccatgg
     4561 tcttaaacca acttctaccc aagttgatga cagggagcaa gctgtattcc cgagttcttc
     4621 gtttctttgg gattggcgag agccagttgg ttacgatttt ggctgattta attgataatc
     4681 agatcgatcc taccttggcc ccttatgcca agacaggaga agtcactcta cgtctgtcaa
     4741 caaaggctag cagtcaagaa gaggcgaatc aagcgctgga tatcttggaa aatcaaatct
     4801 tggactgcca gactttcgaa ggaatttctt tacgagactt ttgctatggt tatggggaag
     4861 aaactagttt agccagcatt gtggtagaag aactgaaaag gcaagggaaa accatcgcgg
     4921 ctgcagagag tttgacggca ggtcttttcc aagctaccgt ggcgaatttt tctggagttt
     4981 caagtatatt taagggtggt tttgtgacct atagcttgga ggaaaaatca aggatgttgg
     5041 atattcctgc caagaatttg gaagaacatg gtgtggtgtc tgaatttaca gctcagaaga
     5101 tggctgagca ggcacgaagc aagacccagt ctgattttgg aattagtttg actggagtgg
     5161 caggaccaga tagcctagaa ggacacccag ttgggacagt cttcataggc ttggcacaag
     5221 atcaaggaac tgaggttatc aaggttaata ttggaggcag aagccgagca gatgtacgtc
     5281 acattgcggt tatgcatgcc tttaacctag ttcgcaaggc tttattaagt gactaacttt
     5341 tgatataata gtagataggt ctgaggatca ttagaatgta ggagaataga atggcgaaaa
     5401 aaccaaaaaa attagaagaa atttcaaaaa aatttggggc agaacgtgaa aaggccttga
     5461 atgacgctct taaattgatt gagaaagact ttggtaaagg atcaatcatg cgtttgggtg
     5521 aacgtgcgga gcaaaaggtg caagtgatga gctcaggttc tttagctctt gacattgccc
     5581 ttggctcagg tggttatcct aagggacgta tcatcgaaat ctatggccca gagtcatctg
     5641 gtaagacaac ggttgccctt catgcagttg cacaagcgca aaaagaaggt gggattgctg
     5701 cctttatcga tgcggaacat gcccttgatc cagcttatgc tgcggccctt ggtgtcaata
     5761 ttgacgaatt gctcttgtct caaccagact caggagagca aggtcttgaa attgcgggaa
     5821 aattgattga ctcaggtgca gttgatcttg tcgtagtcga ctcagttgct gcccttgttc
     5881 ctcgtgcgga aattgatgga gatatcggag atagccatgt tggtttgcag gctcgtatga
     5941 tgagccaggc catgcgtaaa cttggcgcct ctatcaataa aaccaaaaca attgccattt
     6001 ttatcaacca attgcgtgaa aaagttggag tgatgtttgg aaatccagaa acaacaccgg
     6061 gcggacgtgc tttgaaattc tatgcttcag tccgcttgga tgttcgtggt aatacacaaa
     6121 ttaagggaac tggtgatcaa aaagaaacca atgtcggtaa agaaactaag attaaggttg
     6181 taaaaaataa ggtagctcca ccgtttaagg aagccgtagt tgaaattatg tacggagaag
     6241 gaatttctaa gactggtgag cttttgaaga ttgcaagcga tttggatatt atcaaaaaag
     6301 caggggcttg gtattcttac aaagatgaaa aaattgggca aggttctgag aatgctaaga
     6361 aatacttggc agagcaccca gaaatctttg atgaaattga taagcaagtc cgttctaaat
     6421 ttggcttgat tgatggagaa gaagtttcag aacaagatac tgaaaacaaa aaagatgagc
     6481 caaagaaaga agaagcagtg aatgaagaag ttacgcttga cttaggcgat gaacttgaaa
     6541 tcgaaattga agaataagct gttaaagcag tggagaaatc cgctactttt tcgatttttg
     6601 attcaagttt ttagattata tatagtagct tgaaataaga tatgaacaac tttattagga
     6661 aagtcaaatt aatttctaga aatgttttag cagctacagc gtactattcc aaactcaacc
     6721 aattataata gatcgaaact agaatagtac acatctactt ctaaaacatt gttaaaaatc
     6781 gatttgactg tccttatttc attccgctat atatagtttg ctgtttcttg tcgctcctct
     6841 ggaaagctga tataatagct ttatgaataa aaaacgaaca gtggacctga tacatggtcc
     6901 gattcttctc tcgctcttaa gcttcgcctt cccaatcttg ctatcaaata tttttcaaca
     6961 gctctataac actgctgatg tcttgattgt tggacgattt cttggtcaag aatccttggc
     7021 tgcagtagga gcgacgacag cgatttttga cctgattgta ggttttacac ttggtgttgg
     7081 caatggcatg gggattgtca ttgctcgtta ttatggggct cggaatttca ctaaaatcaa
     7141 ggaagcagta gcagccacct ggattttagg tgctcttttg agcattctag ttatgttgct
     7201 gggctttctt ggcttgtatc ctctcttgca atacttagat actcctgcag aaattcttcc
     7261 tcaatcttat caatatattt ctatgattgt gacctgtgta ggtgtcagct ttgcttataa
     7321 tctttttgca ggcttgttgc ggtctattgg tgacagtcta gcagccctgg gatttctgat
     7381 tttctctgcc ttggttaatg tggttctgga tctctatttt attacgcaat tgcatctggg
     7441 agttcaatcc gcaggacttg ctaccattat ttcgcaaggt ttatcagcgg ttctctgctt
     7501 ttattatatt cgtaaaagtg tgccagaact cttgccacag tttaaacatt tcaaatggga
     7561 caaaagcttg tacgcggatc tcttggagca aggtttggct atgggcttga tgagttcaat
     7621 tgtatctatc ggcagtgtga ttttacagtt ttctgttaat acatttggtg cagtgattat
     7681 tagtgcccag acggcagctc gacgcattat gacctttgcc cttcttccta tgaccgctat
     7741 ttctgcatca atgacgacct ttgcttctca gaatctagga gctaagcgac ctgaccgcat
     7801 tgttcaaggt cttcgaatcg gcagtcgttt aagtatatcc tgggcagttt ttgtttgtat
     7861 tttcctcttt tttgccagtc cagctttggt tttcttcttg gctagttcga cagatggtta
     7921 cttgatagaa aatggaagtc tctatctgca aatcagttca gccttttatc ccattttgag
     7981 cctcttgttg atttatcgca attgcttgca gggcttgggg cagaagatcc ttcctctagt
     8041 ttctagcttt attgaactaa tcggaaaaat cgtttttgtg gttttgatta ttccttgggc
     8101 aggatataag ggtgttatcc tttgtgaacc tcttatctgg gttgccatga cagttcaact
     8161 gtacttctca ctattccgtc atcccttgat aaaagaagga aaggcaattt tggcaaccaa
     8221 agtgcaatcc tagttggatt tactgaataa aatccatttc ctctagtgaa catcgaaaaa
     8281 acttgtgttc tcttcttcag tttggtgttg aaaatagttt aacagacttt tgacttcttt
     8341 tatatgatat aataaagtat agtatttatg aaaaggacat atagagactg taaaaatata
     8401 cttttgaaaa gctttttagt ctggggtgtt attgtagata gaatgcagac cttgtcagtc
     8461 ctatttacag tgtcaaaata gtgcgttttg aggttctatc tacaagccta atcgtgacta
     8521 agattgtctt ctttgtaagg tagaaataaa ggagtttctg gttctggatt gtaaaaaatg
     8581 agttgtttta attgataagg agtagaatat ggaaattaat gtgagtaaat taagaacaga
     8641 tttgcctcaa gtcggcgtgc aaccatatag gcaagtacac gcacactcaa ctgggaatcc
     8701 gcattcaacc gtacagaatg aagcggatta tcactggcgg aaagacccag aattaggttt
     8761 tttctcgcac attgttggga acggttgcat catgcaggta ggacctgttg ataatggtgc
     8821 ctgggacgtt gggggcggtt ggaatgctga gacctatgca gcggttgaac tgattgaaag
     8881 ccattcaact aaagaagagt tcatgacgga ctaccgtctt tatatcgaac tcttacgcaa
     8941 tctagcagat gaagcaggtt tgccgaaaac gcttgataca gggagtttag ctggaattaa
     9001 aacgcacgag tattgcacga ataaccaacc aaacaaccac tcagaccatg tggatccata
     9061 cccttacttg gcaaaatggg gcattagccg tgagcagttt aagtatgata ttgagaacgg
     9121 cttgacgatt gaaacaggct ggcagaagaa tgacactggc tactggtacg tacattcaga
     9181 cggctcttat ccaaaagaca agtttgagaa aatcaatggc acttggtact actttgacag
     9241 ttcaggctat atgcttgcag accgctggag gaagcacaca gacggcaact ggtactggtt
     9301 cgacaactca ggtgaaatgg ctacaggctg gaagaaaatc gctgataagt ggtactattt
     9361 caacgaagaa ggtgccatga agacaggctg ggtcaagtac aaggacactt ggtactactt
     9421 agacgctaaa gaaggcgcca tggtatcaaa cgcctttatc cagtcagcgg acggaacagg
     9481 ctggtactac ctcaaaccag acggaacact ggcagacaag ccagaattca cagtagagcc
     9541 agatggcttg attacagtaa aataataatg gaatgtcttt caaatcagaa cagcgcatat
     9601 tattaggtct tgaaaaagct taatagtatg cgttttcttg tggagatatt tccttcaatt
     9661 tttgctacta tattaaacaa aaatcaaaaa gcaaactaga aagttatgct caaataaaat
     9721 ctaaatttga caatgtaaac cgagtcggat agctttaagt actgttttga ggttgaagat
     9781 acgatt
//
LOCUS       DAVKGC010000076         9763 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKGC010000076 DAVKGC010000000
VERSION     DAVKGC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.076"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            7..6021
                     /locus_tag="VW049_001519"
     CDS             7..6021
                     /locus_tag="VW049_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9432609.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAAMGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSESEVSNQKSSVATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSKNTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEPSNGN
                     STEDVSTESNTSNSNGNEEIKQENELDPDKKVEEPEKTLELRNVSDLELYSLSNGTYK
                     QHISLEQVPSNPNSYFVKVKSSSFKDVYLPVASISEERKNDKILYKITAKVEKLQQEI
                     ESRYKDNFTFYLAKKGTEETTNFTSFSNLVKAINQNPSGTYHLAASLNANEVELGPDE
                     RSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEKLSLKNVAISGKDDIGSL
                     ANEAQNNTKIKQVHVDGVLAGERGIGGLLAKAEQSSITESSFKGRIINTYETTAAYNI
                     GGMVGHLTGDKALLTKSKATVAISSNTNTSDQTVGGLAGLVDRDAQIQDSYAEGDINN
                     VKHFGRVAGVAGNLWDRTSGDVRHAGSLTNVLSDVNVTNGNAITGYHYNEMKVKDTFS
                     SKANRVYNVTLVKDEVVSKESFEERGTMLDASQIASKKAEINPLILPTVEPLSTSGKK
                     DSDFSKVAYYQAKRNLTYKNIEKLLPFYNKATIVKYGNLVNENSLLYQKELLSAVMMK
                     DNQVITDIVSNKQTANKLLLHYKDDLSEKLDLKYQNDFAKLAEYSLGNTGLLYTPNQF
                     LYDQTSIIKQVLPDLQKVDYHSEAIRKTLGISPNVKQTELYLEDQFAKTKQQLEDSLK
                     KLLSADAGLASANPVTEGYLVDKIKRNKEALLLGLTYLERWYNFSYGQVNVKDLVLYH
                     LDFFGKGNASPLDTLIELGKSGFNNLLAKNNVDTYGISLASQHGTTDLFSTLEHYRKV
                     FLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYSIGVYDRITSATWKYRNM
                     VLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALNDFVEENARETAKRQRDH
                     YDYWYRILDDNAREKLYRNILLYDAYKFGDDNTVGKATEVADFDNPNPAMQHFFGPVG
                     NKVGHNQHGAYATGDAVYYMGYRMLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGP
                     EFFAKGLLQAPDHPDDATITINSILKHSKSDSTESRRLQVLDPTTRFNNADDLRQYVH
                     NMFDVVYMLEYLEGNSILKLDTNQKQQLLRKVTNEYHPDPDGNKVYATNVVRNLTVEE
                     VERLRSFNDLIDNNILSSREYASGKYERNGYFTIKLFAPIYAALSNDIGTPGDLMGRR
                     IAYELLAAKGFKDGMVPYISNQYEEEAKQKGKTINLYGKTRGLVTDDLVLEKVFNNQY
                     HTWSEFKKAMYQERQDQFDRLNKVTFNDTTQPWQTFAKKTTSSVDELQKLMDVAVRKD
                     AEHNYYHWNNYNPDIDSEVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            6073..6249
                     /locus_tag="VW049_001520"
     CDS             6073..6249
                     /locus_tag="VW049_001520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9432610.1"
                     /translation="MQAGIPTDNEVSYDLIYQQETLPATGSSTSELTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            6480..7331
                     /gene="ylqF"
                     /locus_tag="VW049_001521"
     CDS             6480..7331
                     /gene="ylqF"
                     /locus_tag="VW049_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9432611.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            7318..8097
                     /locus_tag="VW049_001522"
     CDS             7318..8097
                     /locus_tag="VW049_001522"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9432612.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            8113..9663
                     /locus_tag="VW049_001523"
     CDS             8113..9663
                     /locus_tag="VW049_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9432613.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3345 a         1800 c         1990 g         2628 t
ORIGIN      
        1 ggaataatgg aaaagtattt tggtgaaaaa caagagcgtt tttcatttag aaaattatca
       61 gtaggacttg tatctgcaac gatttcaagt ttatttttta tgtctgtatt agctagttca
      121 tctgtggatg ctcaagaaac tgcgggagtt cactataaat atgtggcaga ttcagagcta
      181 tcatcagaag aaaagaagca gcttgtctat gatattccga catacgtgga gaatgatgat
      241 gaaacttatt atcttgttta taagttaaat tctcaaaatc aactggcgga attgccaaat
      301 actggaagca agaatgagag gcaagcccta gttgctggtg ctagcttagc tgctatggga
      361 attttaattt ttgctgtttc caagaaaaag gttaagaata aaacggtatt acatttagta
      421 ttggttgcag ggataggaaa tggtgtctta gtttcagtcc atgctttaga aaatcatctt
      481 ttgctaaatt acaatacgga ctatgaattg acctctggag aaaaattacc tcttcctaaa
      541 gagatttcag gttacactta tattggatat atcaaagagg gaaaaacgac ttctgagtct
      601 gaagtaagta atcaaaagag ttcagttgcc actcctacaa aacaacaaaa ggtggattat
      661 aatgttacac cgaattttgt agaccatcca tcaacagtac aagctattca ggaacaaaca
      721 cctgtttctt caactaagcc gacagaagtt caagtagttg aaaaaccttt ctctactgaa
      781 ttaatcaatc caagaaaaga agagaaacaa tcttcagatt ctcaagaaca attagccgaa
      841 cataagaatc tagaaacgaa gaaagaggag aagatttctc caaaagaaaa gactggggta
      901 aatacattaa atccacagga tgaagtttta tcaggtcaat tgaacaaacc tgaactctta
      961 tatcgtgagg aaactataga gacaaaaata gattttcaag aagaaattca agaaaatcct
     1021 gatttagctg aaggaactgt aagagtaaaa caagaaggta aattaggtaa gaaagttgaa
     1081 atcgtcagaa tattctctgt aaacaaggaa gaagtttcgc gagaaattgt ttcaacttca
     1141 acgactgcgc ctagtccaag aatagtcgaa aaaggtacta aaaaaactca agttataaag
     1201 gaacaacctg agactggtgt agaacataag gacgtacagt ctggagctat tgttgaaccc
     1261 gcaattcagc ctgagttgcc cgaagctgta gtaagtgaca aaggcgaacc agaagttcaa
     1321 cctacattac ccgaagcagt tgtgaccgac aaaggtgaga ctgaggttca accagagtcg
     1381 ccagatactg tggtaagtga taaaggtgaa ccagagcagg tagcaccgct tccagaatat
     1441 aagggtaata ttgagcaagt aaaacctgaa actccggttg agaagaccaa agaacaaggt
     1501 ccagaaaaaa ctgaagaagt tccagtaaaa ccaacagaag aaacaccagt aaatccaaat
     1561 gaaggtacta cagaaggaac ctcaattcaa gaagcagaaa atccagttca acctgcagaa
     1621 gaatcaacaa cgaattcaga gaaagtatca ccagatacat ctagcaaaaa tactggggaa
     1681 gtgtccagta atcctagtga ttcgacaacc tcagttggag aatcaaataa accagaacat
     1741 aatgactcta aaaatgaaaa ttcagaaaaa actgtagaag aagttccagt aaatccaaat
     1801 gaaggcacag tagaaggtac ctcaaatcaa gaaacagaaa aaccagttca acctgcagaa
     1861 gaaacacaaa caaactctgg gaaaatagct aacgaaaata ctggagaagt atccaataaa
     1921 cctagtgatt caaaaccacc agttgaagaa tcaaatcaac cagaaaaaaa cggaactgca
     1981 acaaaaccag aaaattcagg taatacaaca tcagagaatg gacaaacaga accagaacca
     2041 tcaaacggaa attcaactga ggatgtttca accgaatcaa acacatccaa ttcaaatgga
     2101 aacgaagaaa ttaaacaaga aaatgaacta gaccctgata aaaaggtaga agaaccagag
     2161 aaaacacttg aattaagaaa tgtttccgac ctagagttat acagtttgtc aaatggtact
     2221 tataaacaac acatttcgtt agagcaagtt ccaagcaatc caaatagcta ctttgttaaa
     2281 gtgaaatctt cttcattcaa agatgtatac ctaccagtag catcaatatc agaggaaaga
     2341 aaaaatgata aaatccttta taaaatcaca gcaaaagtag agaagcttca gcaggagata
     2401 gaaagcagat ataaagataa ttttaccttc tatctagcta agaagggaac agaagaaaca
     2461 acaaacttta cttcctttag taatctggtc aaagctataa accaaaatcc ctctggaacc
     2521 tatcatttag cggccagcct gaatgctaac gaagtggagc ttggtcctga tgaaagatcc
     2581 tatatcaagg acacctttac tggtcgttta atcggtgaaa aagatggcaa gaattatgct
     2641 atctataatt tgaaaaaacc tctgtttgaa aacttgagtg gtgctacagt agaaaaactg
     2701 agtctaaaaa atgttgctat ttcagggaaa gatgatatcg gttcactggc aaatgaagct
     2761 cagaataaca caaaaattaa gcaagttcac gtcgatggtg ttctggctgg tgaacgtggt
     2821 atcggtggtt tgctggctaa ggctgagcaa tcaagcatca cagagagcag tttcaaggga
     2881 agaattatca acacttatga aacgactgct gcctacaata tcggtggtat ggtcggtcat
     2941 ttgacaggtg acaaggcttt acttactaag tcaaaagcga cagtagccat ttcatctaac
     3001 acaaatactt cagatcagac tgtgggtgga cttgcaggcc tagtagaccg agatgcacag
     3061 atccaagata gctatgctga aggtgatatc aacaatgtca agcactttgg tagagtcgct
     3121 ggagtggcag gcaatttgtg ggatcgaact tctggtgatg ttaggcatgc tggaagtttg
     3181 accaatgttc tcagcgatgt taatgtaacc aacggaaatg ccatcactgg ttaccactat
     3241 aacgaaatga aggtaaagga cacattcagc agcaaggcca acagagtcta caatgtcacc
     3301 ttggtcaagg atgaggtcgt cagcaaggaa tcctttgaag aaagaggaac aatgctagat
     3361 gcttctcaaa ttgcaagcaa aaaagcagaa atcaatcctc tcattctacc aacagtggag
     3421 ccactttcaa caagtggcaa aaaagacagt gatttttcta aggtggccta ttatcaagct
     3481 aagcgcaact tgacttataa aaacattgaa aaattgctac ctttctacaa caaggcaacc
     3541 atcgtcaaat acggaaacct ggtcaatgag aacagtcttt tatatcaaaa agaactcttg
     3601 tcagcagtca tgatgaagga caaccaagtc atcacagaca ttgtttctaa caaacagact
     3661 gcaaacaaac tcttgcttca ctacaaggat gatttatctg agaagctgga tctcaaatac
     3721 cagaatgatt tcgccaaatt agcagaatat agtctgggca atactggact tctctatacg
     3781 ccaaaccaat tcctgtatga ccaaacctct atcatcaagc aagtcttacc tgacttacaa
     3841 aaggttgact atcattcaga agccatcaga aagacgctgg gtatttctcc aaacgtcaag
     3901 caaactgagc tctatctaga agaccagttc gccaaaacaa aacaacaact ggaagacagt
     3961 ttgaaaaaac tcttgtcagc ggatgctgga ctggctagtg ctaaccccgt cactgaaggt
     4021 tatcttgtag ataaaatcaa acgcaacaag gaagccttgc tacttggctt gacctatctg
     4081 gaacggtggt ataactttag ctatggtcag gtgaatgtca aagacctagt tctgtaccat
     4141 ttggacttct ttggtaaggg gaatgcttca ccattagata ctctgattga gttgggtaaa
     4201 tctggcttta acaatcttct agctaagaat aatgtcgata cttatggtat cagtcttgcc
     4261 agtcaacatg gaacgacaga tttgtttagc acgctggaac attaccgaaa agtcttttta
     4321 ccaaatacaa gcaataatga ctggtttaaa tcagagacta aggcttacat tgtcgaagaa
     4381 aaatccacta tcgaagaggt gaaaacgaag caagggttag ctggcaccaa gtattctatc
     4441 ggtgtttatg atcgtatcac gagtgccaca tggaaatacc gcaatatggt cttgcctctc
     4501 ctgaccttgc cagagagatc cgtatttgtc atctcgacca tgtctagtct aggatttgga
     4561 gcttatgatc gctaccgcag tagtgaccat aaagcgggca aggctctcaa tgattttgtt
     4621 gaagaaaatg cgcgtgaaac agccaaacgt cagcgagatc actacgatta ttggtatcgt
     4681 attttagacg acaatgcacg tgaaaaactt tatagaaata ttttgcttta cgatgcttat
     4741 aaatttggcg atgataatac cgtagggaaa gctacagaag tggcagattt tgataatcca
     4801 aatcctgcaa tgcaacattt ctttggacct gttggaaata aagttgggca taatcaacac
     4861 ggtgcttatg ctacaggtga tgcagtttat tatatgggtt atcgaatgtt ggataaggat
     4921 ggagctatta cttatacgca tgagatgaca catgactcag atcaggacat ttatcttgga
     4981 ggatatggtc gaagaagtgg cttgggacca gagttctttg ctaaaggatt attacaagca
     5041 ccagaccatc cagatgatgc gaccattacc atcaactcca tcttgaaaca ttcaaaatct
     5101 gatagtacag aaagtcgacg attacaagta cttgatccaa ctacaagatt taataatgca
     5161 gatgatttga ggcaatatgt ccacaacatg tttgacgttg tttatatgtt ggaatatctc
     5221 gaaggaaatt caattcttaa attggatacg aatcaaaaac aacaacttct tagaaaagtt
     5281 acaaatgagt accatcctga tcctgatgga aataaggtct atgcaacaaa tgttgtcaga
     5341 aatctaacag tagaagaagt tgaaagacta cgttcattca atgatttgat tgataataat
     5401 attctttcgt ctagggaata tgcctcaggt aaatacgaaa gaaatggcta cttcactatt
     5461 aagttatttg caccgattta tgctgcatta agtaatgata taggaacacc aggtgacctg
     5521 atgggacgtc gtatagccta tgaactacta gctgctaaag gctttaaaga tggtatggta
     5581 ccatatatct caaaccaata cgaagaagaa gccaaacaaa agggcaagac aatcaatctc
     5641 tacggtaaaa caagaggttt ggttacagat gacttggttt tggaaaaggt atttaataac
     5701 caatatcata cttggagtga gtttaagaaa gctatgtatc aagaacgaca agatcagttt
     5761 gatagattga acaaagttac ttttaatgat acaacacagc cttggcaaac atttgccaag
     5821 aaaactacaa gcagtgtaga tgaattacag aaattaatgg acgttgctgt tcgtaaggat
     5881 gcagaacaca attactacca ttggaataac tacaatccag acatagatag tgaagtccac
     5941 aagctcaaga gagcaatctt taaagcctat cttgaccaaa caaatgattt tagaagttca
     6001 atttttgaga ataaaaaata gtgtctacta ttaggaaata aagtttaaaa aggtgatgaa
     6061 gaacaaacta agattcaagc aggaattcct actgataatg aagtaagtta tgatcttatt
     6121 tatcagcagg aaactcttcc tgcaacaggt tcatcaactt ctgagcttac agctttaggc
     6181 ctattagctg ttggtagttt agttcttttg gttcataata tgacgggaac agttttttgc
     6241 tccctctgaa aagtcatcat ttgatggctt ttttctatat agggtaaaag atagggtaaa
     6301 agatagggta aaaggctatc atcggacaaa ataaagaagg catgatataa tataaagtag
     6361 atttctatgt cataaaacaa gaactgtttg gacatcattc atttgaaaac tctctatgtt
     6421 caaacaatag taaaataaaa taggggatct aaatccttgc tatgaaagga aaaaactcaa
     6481 tggctactat tcaatggttt cctggtcaca tgtctaaagc tcgtcgacag gtgcaggaga
     6541 atttaaaatt tgttgatttt gtgacgattt tagtagatgc acgcttgcct ctatctagtc
     6601 aaaatcctat gttgaccaag attgttggtg ataaaccaaa actcttgatt ttaaacaagg
     6661 ccgacttggc tgatccagca atgaccaagg aatggcgtca gtattttgaa tcacaaggaa
     6721 tccagacgct agctatcaac tccaaagagc aagtgactgt aaaagttgta acagatgcgg
     6781 ccaagaagct catggctgat aagattgctc gccagaaaga acgtgggatt cagattgaaa
     6841 ccttgcgtac catgattatt gggattccaa acgctggtaa atcaactctg atgaaccgtt
     6901 tggctggtaa aaagattgct gttgttggaa acaagccagg ggtcacaaaa ggtcaacaat
     6961 ggcttaaaac caataaagat ctggaaatct tggatacacc ggggattctc tggcctaagt
     7021 ttgaggatga aactgttgca cttaagttgg cattgactgg agctatcaag gatcagttgc
     7081 ttcctatgga tgaggttacc atttttggta tcaattattt caaagaacat tatccagaaa
     7141 agctggctga acgcttcaaa caaatgaaaa ttgaagaaga agcgcctgtg attattatgg
     7201 atatgacccg cgccctcggt ttccgtgatg actatgaccg tttttacagt ctcttcgtga
     7261 aggaagtccg tgatggcaaa ctcggtaact ataccttaga tacattggaa gacctcgatg
     7321 gcaacgatta aagaaatcaa agaactcctt gtgacagtca aggagttaga aagccctatt
     7381 tttttagagc ttgaaaagga taatcgctca ggagttcaaa aggaaatcag caagcgtaaa
     7441 agagccattc aagctgaatt agatgaaaat ttgcgcttgg aatccatgct ttcttatgaa
     7501 aaagaacttt ataagcaagg attgacctta attgcaggta ttgatgaggt tggtcgtggt
     7561 cctcttgctg gtcctgtagt cgctgcggcc gttattttac ctaaaaattg taagattaaa
     7621 ggtctcaacg acagcaagaa aattcctaaa aagaaacatc tggagatttt ccaagccgtt
     7681 caagaccaag ccttgtcgat tggaattggt atcatagata atcaggtcat cgaccaagtc
     7741 aacatctatg aagcaaccaa actagccatg caagaagcaa tctcccagct cagccctcaa
     7801 ccagagcacc ttttgattga tgccatgaaa ctggacttgc ccatttcaca aacctccatt
     7861 atcaaaggag atgccaactc cctctctatc gcagcagcat ctatagtagc caaggtaaca
     7921 cgtgatgaat tgatgaaaga atacgatcag cagttctctg gctatgattt cgctactaat
     7981 gcaggatatg gcacagctaa acatctggaa ggcctcacaa aactaggagt taccccaatt
     8041 caccgaacca gctttgaacc cgttaaatca ctggttttag gtaaaaaaga aagttaattg
     8101 aaaggaaata ccatggagga acagtcggaa atagtccgtt ctaagaaaga attcgccttt
     8161 gcatccagca ctatactatc ccaagttggt cgaggaatca ttgtcggcct catcgttgga
     8221 attatcgtcg gatcctttcg tttcttaatt gaaaagggct tccacctgat acaaggagtt
     8281 tatcaagatc aagggtactt agtgcgcaat ctttttgtac tggttttgtt ttatatactc
     8341 atctgttggc tcagtgccaa actaacacgg tcagaaaaag atattaaagg ctcaggaatt
     8401 cctcaagtcg aagccgaact gaaaggcctc atgtccctca actggtgggg cattctttgg
     8461 aaaaaatatg tgctaggtat tcttgctatt gccagtggac tcatgctggg tcgagaggga
     8521 cccagcattc aacttggagc agttggtggt aaaggaattg ccaagtggct caaatccagt
     8581 ccagtagagg aacgttcctt gattgccagt ggagctgcag caggtttagc cgcagccttt
     8641 aatgctccta ttgcagcact tctctttgtt gtagaagaag tctatcacca tttttcgcgc
     8701 tttttctggg tctcaactct agcagccagc atcgtagcaa actttgtgtc tctactcatg
     8761 ttcggtttga caccagtatt ggatatgcca gataacattc ctcccatgac cctagaccag
     8821 tattggatat atctcgtcat gggaattttc cttggatttt caggttttct ctatgagaaa
     8881 gctgtattaa acgttggaag agtttatgac ttgattggtc aaaaaatcca tttggatagg
     8941 gcttattatc ccatcttggc ttttatcctt atcataccag tcggaatctt cttacctcaa
     9001 atcattggtg gcggaaatca gcttgtcctt tctttaactg aacaaaattt tagtttccaa
     9061 gttttattag cttacttttt aatccgcttt atttggagta tgattagcta tggaagtgga
     9121 ctgccaggag gaattttcct ccccatttta gctcttggtt ctttgcttgg tgccttagtt
     9181 ggtgttatct gtgtcaatct tggacttgtc agtcaagagc aattccctat atttgtcatt
     9241 ctaggaatga gtggctattt tggagccata tcaaaagctc ccttaaccgc tatgatcctc
     9301 gtaactgaga tggtaggaga tattcgcaac cttatgccac ttggtcttgt cactcttgtt
     9361 tcttatatta tcatggattt gctcaaaggt acgccagtct atgaagccat gctggaaaaa
     9421 atgcttccag aagaagtatc tagcgaagga gaagttacac ttatcgaaat accagtttct
     9481 gataaaattg ctgggaaaca agttcatgaa ctcaacttac cacacaacgt cctcatcaca
     9541 actcaagtcc ataatggcaa gagccaaaca gttaacggct caaccagaat gtatctgggt
     9601 gatatgattc acctggttat tccaaaaagt gaaattggaa aagtcaaaga tttgttgttg
     9661 tagtatgatc atttacataa tttatgttat gtaaatgatc agtttgattt atttagaaaa
     9721 ccgattctca gaaatgagat cggttatttt ttactgatga gga
//
LOCUS       DAVKGC010000077         9540 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKGC010000077 DAVKGC010000000
VERSION     DAVKGC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9540
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.077"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            40..366
                     /locus_tag="VW049_001524"
     CDS             40..366
                     /locus_tag="VW049_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9432614.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            353..946
                     /locus_tag="VW049_001525"
     CDS             353..946
                     /locus_tag="VW049_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9432615.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            939..1850
                     /locus_tag="VW049_001526"
     CDS             939..1850
                     /locus_tag="VW049_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9432616.1"
                     /translation="MRKWTKGFLIFGVVTTIIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            1913..2977
                     /locus_tag="VW049_001527"
     CDS             1913..2977
                     /locus_tag="VW049_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432617.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTKIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPYQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(3049..3909)
                     /locus_tag="VW049_001528"
     CDS             complement(3049..3909)
                     /locus_tag="VW049_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9432618.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIYLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQLEIQPILG
                     QDFKTLAQEK"
     gene            complement(4031..5017)
                     /locus_tag="VW049_001529"
     CDS             complement(4031..5017)
                     /locus_tag="VW049_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9432619.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMENLWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(5289..6764)
                     /locus_tag="VW049_001530"
     CDS             complement(5289..6764)
                     /locus_tag="VW049_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9432620.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEVLNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(6848..7771)
                     /locus_tag="VW049_001531"
     CDS             complement(6848..7771)
                     /locus_tag="VW049_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9432621.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(7785..8714)
                     /locus_tag="VW049_001532"
     CDS             complement(7785..8714)
                     /locus_tag="VW049_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9432622.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(9033..9254)
                     /locus_tag="VW049_001533"
     CDS             complement(9033..9254)
                     /locus_tag="VW049_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432623.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(9208..9468)
                     /locus_tag="VW049_001534"
     CDS             complement(9208..9468)
                     /locus_tag="VW049_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432624.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         2845 a         2007 c         1818 g         2870 t
ORIGIN      
        1 atatataaaa atcttacaca ttagaaaagg aggtttccca tgtactttcc aacatcctct
       61 gccttgattg aatttctcat cttggctgta ctggagcagg gtgattctta tggttatgag
      121 attagccaaa ccattaagct gatcgctaat atcaaagaat ccacactcta tcccattctc
      181 aaaaaattgg aaggcaatag ctttctgaca acctattcta gagagttcca aggtcgcatg
      241 cgcaaatact actccttgac aaacggtggt atagagcagc tcttgaccct aaaagatgaa
      301 tggacactct atacagacac catcaatggc atcatagaag ggagtatccg ccatgacaag
      361 aactgaatac ctgactcagc tagaactcta tctcaagaaa ctacctgaag ctgaccgtat
      421 cgaagccatg gactatttca gagagctctt tgacgatgct ggagtcgaag gagaagaaga
      481 actcatcgct agtttgggaa ctcccaaaga agcggcccac gaagttctat ccaatcttct
      541 cgataaaaaa atcaatgaag cacccgctca aaaaaataac cgacaaattt tacatatcgc
      601 cttgttagcc ctccttgcag cacctatcgg cattcctctg ggaatcgcca tcctcgtgac
      661 cctgttcgca atccttgtag ccgctttgac tgtcattctg gctttctttg cagtttccat
      721 actgggtatc atcggcggat tcctattttt agttgaaagt ttcactatcc tcgcccaagc
      781 caaatcagcc tttatcttga tttttggttc tggtttactg gctatcggtg cttcttcgct
      841 agttttactt ggcatttcct atgtagctcg cttcttcggt ctactcattg ttcgtctggt
      901 acaatttgtt cttaaaaaag gaaagagagg taatcagcat gcgtaaatgg acaaaaggat
      961 ttctcatctt tggtgtggtg actaccatta tcggctttat cctgcttttt gtaggtatcc
     1021 aatctgacgg gattaagagc ctactttcca tgtccaaaga acctgtctat gatagccgta
     1081 cggaaaagct aacctttggc aaggaagtcg aaaacctaga aattactctc caccaacacg
     1141 cgctcaccat cacagactct ttcgatgatc aaatccacat ttcttaccat ccatctcttt
     1201 ctgctcacca tgatcttatc accaatcaga acgatagaac tctgagtctc actgataaga
     1261 aactgtctga aactccgttt ctctcttctg gaattggtgg gattcttcat atcgcaagta
     1321 gctactctag tcgttttgaa gaagttattc tccagctacc aaaagggaga actctaaaag
     1381 ggatcaacat ctcagccaat cgcggacaaa ccaccatcat aaatgctagc cttgaaaatg
     1441 cgaccctcaa tacaaacagc tatatcctcc gaattgaagg aagtcgtatc aaaaacagta
     1501 aactcacaac gcccaatatc gttaatatct ttgatacagt tcttacagat agtcagctag
     1561 agtcaacaga gaatcacttc cacgctgaaa atatccaagt ccatggcaag gttgaactga
     1621 ctgccaaaga ttatctcaga atcatcctag accagaaaga aagccaacga attaactggg
     1681 acatctcaag caactatggt tctatcttcc aattcacaag agaaaagcct gaatcaagag
     1741 gtacggaatt aagcaaccct tacaaaactg aaaaaaccga tgtcaaggat caactcattg
     1801 cgagatctga tgataatatt gatctaatat ccacaccaag cagacgttga caaaaacgct
     1861 tacatctgct actatgaaag catatttatc taactaaaaa ggaggttcta ccatgaaaca
     1921 agaatggttt gaaagtaatg attttgtaaa aacaacaagc aagaacaagc ctgaagagca
     1981 agctcaagag gttgcagaca aggctgaaga aacgatagcc gatctcgata caccaattga
     2041 aaaaaatact cagttagagg aggaagtctc tcaagctgaa gtcgaattgg aaagccagca
     2101 agaagagaaa attgaagctc ctgaagacag tgaagcgaga acaaaaatag aagaaaagaa
     2161 ggcatctaat tctactgaag aagagccaga cctttctaaa gaaacagaaa aagtcactat
     2221 agctgaagag agccaagaag ctcttcctca gcaaaaagca actacgaaag agccacttct
     2281 tatcagtaaa tctttagaaa gtccttatat cccctaccaa gctccaaaat ctagggataa
     2341 atggaaagag caagtgcttg atttttggtc ttggctagtg gaagcgatca aatctcctac
     2401 aagtaagttg gaaacaagta tcacacacag ttacacagcc tttctcttgc tcattctgtt
     2461 ttctgcatct tcctttttct ttagtatcta tcacatcaaa catgcttact atggacatat
     2521 agcaagcatt aacagtcgct tccctgagca gctagctcct ttaactcttt tttctatcat
     2581 ctctatccta gtagcgacaa cactcttctt cttttcattc ctcttgggta gtttcgttgt
     2641 gagacgattt atccaccagg aaaaggactg gacgctagac aaggttctcc aacaatatag
     2701 tcaactcttg gcaattccaa tcttcctcac tgctattgct agtttctttg ctttctttga
     2761 tagcctacga tttacagctc tcttgtgtgt aattagcatt ggaatcattc tgcttgccag
     2821 tctccatatc attacaagac caagtcaagc aagtgaaacc gactccttct atcaattatt
     2881 cttgtctgtc cttgtgaacg gagttattat cctcctcttc tttgtagctg aagtggcact
     2941 gattggagat tatcttcgta tcttggcctt tctttaaaat tcaatcctgt catccttggc
     3001 aggatttttt ctatgtcaaa aaagcaagtc gattgacctg cttctgcttt acttttcttg
     3061 tgctaatgtt ttaaaatctt gtcctaggat gggttgtatt tctaattgat tggcgtctag
     3121 ctggtggtaa aaagatgggg gtaacacctc taagaatttt gcatagtcca agcgagctat
     3181 ggattcatag tcttctggtt tagagccgtc tcctgtgatt tgaaccaagt aaatctccca
     3241 ctggacttcc ttatccacca attgctcaat ataggctgca ggaacaaacg gttctctcgg
     3301 taaaactgtc ttgactcctt gagctaaatg gtaaacgcca tgcacttttc cttctccatc
     3361 ctgatagaag gaaactcttg caaagtaggc atctgtctct tgaaccgcac gcacacgcgc
     3421 ttcaaactga gccaagccat ctgcctctaa aaagcttttc aagttctcac gactaaagaa
     3481 aataggatta aaaattcctt ggcaaatcgt aaaacgagct acagggtctg ctagataggc
     3541 ttgaatactt gcttggaaat aagcttcaaa atcaaaacca tctagccctt caatatcagt
     3601 ccctgtataa gcaagcaaag catctaaaaa tgacttgata atctgccagt ctgtctttgt
     3661 tagtaggtca ggaagatcga cacggtaagc cttttgacca tcagcataac tgaccttaaa
     3721 gagaaattgt gattgcccca ctatcgcaca ctcaatatac tcgagacgat taagaggctg
     3781 acgaagatag actacatcat agctatgtga ctccaaaccg tctagcaagg ccaagatcga
     3841 cttggcattc aaaatctctt gttctcctaa aatgctctgt ttatttggaa taaaaaatgt
     3901 ttttgccata actggactcc tttgaaatcg tttacataca gtatacctta ttttcctgaa
     3961 agatacaaaa acaaacttta gatttttgag tacaaagcat agaaaaacag cccctgagga
     4021 ctgtttgatc ttatacagta gctgcctgat ccaagctttc accgatagcg gctaggcgct
     4081 caatcacttc agcttgtgtc aattcatttt ttgaaacata gcggttacgt gggtgaacac
     4141 gacactcgtg tgagcatcca cgaaggtact tgtcttcatt ttcttctgat gtcaagatac
     4201 gacggttaca gaagggattt ccacagttga cataacgttc acatggtgtt ccatcaaacc
     4261 agtctttccc tacgatggtt gggttgacat ggttgacatc aacggcaata cgctcgtcaa
     4321 agacgtacat tttcccatcc caaagctcac cttgaacttc tgggtcttta ccgtaagttg
     4381 cgattcctcc gtgcaattgg ccgacatctt tgtagccttc acggaccatc cagcctgaga
     4441 atttctcaca gcgaacgcca cctgtacagt aaaccacgac acgcttgtcc atgaattttt
     4501 ccttgttatc acggacccat tgtggtaact cacggaagtt gcgaatatct gggcgaatag
     4561 ccccacggaa atgtcctagg tcgtactcat aatcgttacg tgtgtcaagg acaacggtat
     4621 ctttatcaag aagcgcttct ttgaactctt ttggagacaa gtaagcacct gttgtttcaa
     4681 gtgggttgat gtcattgtca aagtcgttgt cttccaaacc aaggtggaca atttctttct
     4741 tgtagcgaac aaacatcttc ttgaaggctt gttcactttc ttcgtcaatc ttgaaccaga
     4801 gattttccat tcctggaagg ctgtgaacgt agtccatgta tttttgagtt gtttcatagt
     4861 cacctgaaac tgttccgtta attccctcgt cagcgactag gatacggcct ttaaggccga
     4921 ttgatttaca gaaagccaag tggtctgcag caaattgctc tgcattttca attggagtat
     4981 agaggtagta aagtaagaca cgaatatctt ttgccataag atttgttctc ttttctattc
     5041 ttaaattttc taaatttttt atttatccat actagtatac ctgcttgaga aaacgaatgc
     5101 aatttatttg actggaaatt gtagagggtt ctcgaaattt cctgaatggt taaaataagg
     5161 acaagagaaa acatggatat ctatatcctt gtgccaaaaa aaccactgcc ctccccagac
     5221 caacctgagg aaagcggtga ttcttatttt aggagttagg aatgaataca cgaaatcaat
     5281 ttagctgatt attttttgtt tttcaagaat tcatcgtatt gtttttgcat ttcgttcaat
     5341 actttttcgt aggcaccttc agatttcaat ttttccatca attctggaat agctttatct
     5401 gggtctacag taccagtgtt gatagctgta tcaaattgtt gcattgtgtt agcaatagct
     5461 gagatttcag atttcacatt gtcagtattg aagataaatc caagcgctgg agattcttta
     5521 gcttctgcca attctttctt agaattttcg atttgttggt ctgtaacgtt ttcgttgatg
     5581 taaaggatcc agttgttacc agtgttccat ccacccatgt gagtgtttcc tttgtagcca
     5641 tcaagaacgc gaacacgatt ttctttacct tcaatttttt cccagttctt gccttctgga
     5701 ccgtaaacaa gaccgttcaa gagttctggg ttcgtattca agaggttcaa cacttccatt
     5761 gatttttctt tgttcttaga gttgtttgag atgacaaagt tagcaacttg tgttgtttgg
     5821 tttttcttga tgaagttagt aattggtttg atttggatat ctttgttggc aacacgtgaa
     5881 agcaagctgt taccgtagtc agctggtcct actgtttctt cacgaacgaa ccaagtatct
     5941 tgttgaaggt caaatgaagt atcgcttgtt gcgacatctt ttggaatgta gccagcttca
     6001 tagaatttgt gaagagtctt caagtgttct ttgaaacgag gcacttcgta acggtttaca
     6061 actttagtag tatcgccttc aagatcaata acgaatggaa gcccgtttgc tactgggtag
     6121 tcaaagttat cagatgggat gaaaacttta ccaatagcaa atggtactac gtctggagct
     6181 ttttctttaa tttgtttcaa gactggctca agagtttcgt aagaagtaac acctgaaata
     6241 tcgataccat atttggcaag gagagttccg ttgaaggcaa agttttgaga tgatgcaacg
     6301 ttggctgcaa ctggaacagc gtaaatttta ccgtttacag tattaccctt gatgtaagct
     6361 gggtcaagtg ccttgtaaag gtctttacct tcttttttgt acaattctgt caagtcagca
     6421 taagcacctt tttgagcatt tacaatatag ttatctgcaa aggcgatatc atagttttca
     6481 ccagatgatg tgataactga cattttctta ccatagtcac cccagccaag gtattggata
     6541 tccaatttgg caccaacttt ttcttcgatg attttgttgg catttgctaa caattcatcc
     6601 aagttatctg gtttgtcacc gatttggtac attttgataa caggtttgtc acctgaatca
     6661 gcagcttttt tgctgttacc tgtcaaattt ccacaagcag caagacctgc agccaaagcg
     6721 actacactag cagatgcaaa agcatatttt ttccagtttt tcatgataaa aactcctttt
     6781 tttattttta aacttataaa caatgtaatg atcttaactt cacgcaaggg aagtttggaa
     6841 acaagtgcta ttctttcaca ccaccgatag tcaaaccttt tacaaagtag cgttggaaaa
     6901 atggatacaa aatcgcgatt ggaagggttg caaccacaac catggccata cgacctgttt
     6961 ctttcggtag agcaactccc agttgaccag tcaagccgac cgctttggca atgtagtcca
     7021 tattttgttg gatttgcatg agcaaatatt gcaatggata caagttgtca ctcttgatgt
     7081 aaagaagggc gttgaaccag tcattccaga aaccaagagc tgttaagagc gtgatggttg
     7141 cgatacctgg tagtgacaat ggcaaacaga tttggaagaa aatccgggct tcactggcac
     7201 catcgatacg agccgattct agaatggctt ctggaatggt cttcttgaag aaggaacgca
     7261 tcaagatgat gttaaatggt gagagaagca ttggaacaat caaggcccaa acagtatccc
     7321 caagctgaag tacacgggtc accatgatat aacctggtac caaaccagcg ttgaacaaca
     7381 tactgagaag gacgaagatg gtaaaaaatc tgcgatactt aaaggttgtc cgtgaaatag
     7441 cgtaggcata ggttgttgtg ataaagacat ttgtcaatgt cccaactacg gttacaaaga
     7501 cagagatgaa gagggcttgt aggattttat ccttaaactg tgccaaaaac tcaaaaccgt
     7561 ctaagccaaa ttgggatggg aagaagctat agccgtattg gaggaggctt ttctcgtctg
     7621 tcactgaaat aatgataacg aatacaaaag gtaggataca agagagggca atcaaacccg
     7681 aaatgatact gaagaagata tctgctttct tactgaagga gtgaatgccg acattatcaa
     7741 ttttttcttt tttaattttc ttttttgcca tattctcctc ctttctagaa caaagctgag
     7801 tttggatcga ctcgtcttgc aagcaagttt gataggataa ccagaatcaa accaacaacg
     7861 gattggtaaa gaccggctgc tgcagccata ccgatatctg ctgtctgagt caaaccatta
     7921 aagacatata cgtccaaaac gttggttaca ttgtaaagct gaccagcatt gtgtgggatt
     7981 tgatagaaga gaccgaagtc tgcgcggaag atatttccga ctgcaaggat ggtcaataca
     8041 gttacaagcg gagtcaactg aggaatggtt acgttgcgaa tacgttgcca cttgctagct
     8101 ccgtccactg tcgctgcttc gtagtaggtt ggatcaattc ccatgatcgt cgcatagtac
     8161 atgacactgc tatatccaaa gcctttccaa atacctagga aaagtaggag atagggccag
     8221 atgcccaggt cagcgtaaaa attgacttct ttgagaccaa gactttccaa tagatgattg
     8281 aacacccctt tatcaatatt taggaaggca tctgtaaaga aactgatgat aacccaagac
     8341 aagaagtaag ggaacaacat agaagtttgg aaaatcttca ccattctctt agaacggagc
     8401 tcgctgagga taatggcaat ccctacagat acaactaaac ctagaaagat aaagccaaga
     8461 ttgtagagga cagtatttcg tgtgataata aaggcgtctc ttgaactaaa taagaatctg
     8521 aaattatcga gtccgaccca tttactattt atgatactat ctatgaaacc attactggtc
     8581 atgtggtagt ctttgaaggc aaccacgttc ccaaatactg gaatgtaaaa gaatagaatc
     8641 aaccagagtg cccctggcaa aaccatcaag agaaagatcc agttgtctct caatgttttt
     8701 gaaaactttt tcataatttc ctcccttttt attttgatat ccatctaaaa attctttttt
     8761 agacttttga taacgattac attattagta tactcctatt tgtaggttag gttaaactcc
     8821 taattataga aaaaactcca caaattatgt agcagattta aaactttatc accactatca
     8881 aacaaatgtc ctaaatcaat tgtttatttt atctctatta gcccagtgat ggcgtcactc
     8941 tgttataaac atccaacaac gggggatact gaaaaatctc cagactaggg aactcagcga
     9001 tagttcctaa tctggagatt tttaatatgt tattaggcgt ttgctttcaa cttagcaata
     9061 acctctttaa gattatcaat cacctctgct tcagtatgct cagagccttt ttcatctgcc
     9121 aagaacaaaa ctgctttttg aagttctttt tgagagtttt caaggacatc cttatctact
     9181 gtttcaaggt ttgagtcttt aagaagttta cttaattcct tggctaattt cttgagtttg
     9241 atttgcagac tcatcttctc ctgctgtttc tttgcccgct gtttgtcctc catccttagt
     9301 tgctgactgg ctttccttaa tggactctag ggaagcaatg gcatctttga ctgtttgcaa
     9361 gatatcacgt aaaccttgct ctgtcaaact atcatctgca aaagctttct tagcctctgc
     9421 caaaaccaga cgtgctgaat ctgtggtagg attcgataca cctgtcaatg atctcaaaag
     9481 attttctaag gtttgagtct gcttactaat actagactaa aatcaaaaag tattatataa
//
LOCUS       DAVKGC010000078         9334 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKGC010000078 DAVKGC010000000
VERSION     DAVKGC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.078"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(193..813)
                     /locus_tag="VW049_001535"
     CDS             complement(193..813)
                     /locus_tag="VW049_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9432625.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPGSIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(813..1295)
                     /locus_tag="VW049_001536"
     CDS             complement(813..1295)
                     /locus_tag="VW049_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9432626.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKMSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1295..2137)
                     /locus_tag="VW049_001537"
     CDS             complement(1295..2137)
                     /locus_tag="VW049_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9432627.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2232..3032)
                     /locus_tag="VW049_001538"
     CDS             complement(2232..3032)
                     /locus_tag="VW049_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9432628.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPFYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3176..4555
                     /gene="glmU"
                     /locus_tag="VW049_001539"
     CDS             3176..4555
                     /gene="glmU"
                     /locus_tag="VW049_001539"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9432629.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVILKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVIVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGNNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4565..5110
                     /locus_tag="VW049_001540"
     CDS             4565..5110
                     /locus_tag="VW049_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9432630.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5122..5436
                     /locus_tag="VW049_001541"
     CDS             5122..5436
                     /locus_tag="VW049_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9432631.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5453..6145
                     /locus_tag="VW049_001542"
     CDS             5453..6145
                     /locus_tag="VW049_001542"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9432632.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6222..6713
                     /gene="rocS"
                     /locus_tag="VW049_001543"
     CDS             6222..6713
                     /gene="rocS"
                     /locus_tag="VW049_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9432633.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            6811..7401
                     /locus_tag="VW049_001544"
     CDS             6811..7401
                     /locus_tag="VW049_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9432634.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7410..9173
                     /locus_tag="VW049_001545"
     CDS             7410..9173
                     /locus_tag="VW049_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9432635.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINFLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFEFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         2870 a         1628 c         2001 g         2835 t
ORIGIN      
        1 attgatttaa attctcaaat catattattc agttcttatt tcattttgct ctacaatcct
       61 gttgagaaga cacgtgttca tatcaaaaag gtattggcaa gttgcaatac ctttttacaa
      121 ggttcttttg tcttattttt gtttcaactg actatatctc ctatggttct agttcagaag
      181 gctaggctat aattatgatt gataagaagt atcattccaa gtattgagag tgaatgtttc
      241 aaaatcatgg gtttctataa tggtcaggct ggcatttgct agaccgccat ctttacgaag
      301 aagtggttct ttatagccta ggagagtacg aagactggca gtaagattgg cgccgtgtcc
      361 gacaattaga atacgctcag ctggactatc ttttaatgat ttgataaatt ggatggtccg
      421 ctgagttgta ctatagaggg attcggctcc gaacattcga gtgtcaaatt gagcaagatt
      481 tgaacgaaaa gcctggattt gttgcgggta aatagcttcc agggttgcaa ttttcaaacc
      541 ttctaacttc ccaagttgcc attcacggag attaggaacg atttctaaag aacagggggt
      601 atggagttga ctttggataa tctcagcaga tttgaccgct cgaggtaaat cacttgaata
      661 aatctgatca aaaggaattt ccttgagata ctggccaagt cgttttaggg tttcaatgga
      721 tccaggaaga agaggagaat caccgctagc accttgaaaa cgaccttctt ggttccagag
      781 ggtacgaccg tggcggacaa agtagagttt cattacttga tgtcctccaa aatatctaca
      841 aagtctgcct ttacaaagct agccaagtct tgtggcgcga cgataatgct gtgtccgact
      901 tcgcctgcag agacaatcat tcgatccaaa tctagagcaa ttttatcgat aaaaatggga
      961 taattgtgtt tctgacgaat tccgacagga ttattggctc catgaatgta accagttgtt
     1021 ttttctaagt ccttttgtgg aatcatgctc atttttttat tgccagaaat tttagctagt
     1081 ttcttttcag acaagtgctg agtgataggg acaattccga taatcggtcc ggtcttgtct
     1141 cccaaaagcg ccaaggtttt gaaaatctga tctcgttcat aaccttgagg aagctctcct
     1201 tctagggcat tgatttgaat cccctgatga gggatagctg ctttagatag gatttgttcc
     1261 accaatgttt ttttgatttt aacttttttt gccattattt atatttatcc tccaattgac
     1321 tcatccaaat accaagccag attcccagcg caaagaagaa ggcgatgatg acataaccga
     1381 caagtgaaag tcctgtgtat tggatacttt cagcgtttcc tgcatttgga attaagatca
     1441 aaagggtact tgataggacg ataccgatga tgaaatgata gacgcgtgag tggtagttgt
     1501 ttaaggcata atccatcaat tttgaaaaaa cgatgagagt tgcacctgca ccaattccaa
     1561 tcggaaagaa ggttcccaag agatcaaaag ttttaaaacc agtcaacata ggagcataga
     1621 gtcccaaaat caaaagtaaa ttcgatgggc tgaggccagg aaccaagacg ccaagggcca
     1681 atagtgcgcc tgctaggacg aagttaagaa agctggcgct taaggttcca acgacaaaat
     1741 ttaaggcata gagtcctaat ccagaaatga taaaggttgt ccataaccaa gctaaatcaa
     1801 tcttgtctcg gtcagattct cgagttgatt ctttgaggag gctaggaact gtaccgataa
     1861 tagctcccgc aaagctccat aatacaaaga cctgataatt ttcaagcagg tattcaatcg
     1921 ggtaggaaaa taagccgatt cccagaagca taccgatggc aactggaata aagtacaaaa
     1981 cattttcttt aaagtcttta aagggatggg ccagaaagcc aatcattcgt tcatagattc
     2041 ctaagattgc tgctagaacc cctccggaaa ttcccggtag gataaatcca agagcaatta
     2101 caatcccttt aataacgcgt gctaaccatg agagcatata tttcctccaa ctatttctat
     2161 ttcaaaaaat ccttactata ttgtatcaca atcagacaca aaaagaaaaa gcaaatgata
     2221 aacaaatgct tttaaggttt taaaaaaagt tttcgaaagg tttcttcttt attttttaag
     2281 ggagagataa cgttgatatc taaatcgtgg tcaaagccgg caatttttcc tttagatgtg
     2341 tattggtgaa tatcataatc taaatcagtt ttaggactgc tttccaaaaa tcctgagtct
     2401 gagccgtaga acggaatcca aacagaggta aacttgcctg tatcaatact gtgttcttcc
     2461 atgaagtaga caccaacgta gatgccgatg tttttagcac ccagtgatgc tagttttgct
     2521 cgaaagtttt cgacaccttc gttcatatta gacatggttt tgtcttccac gtcaagccaa
     2581 tagtaactag ggctgtaagg agaggcagca ttgtagaaaa cttcggcagc cttttccatt
     2641 tcttggacac tttttccagc tacataagcg tagacagcaa ctgggacatt ccgcttttga
     2701 agttcagtga tatgactctt ataggccttg tctattccat tgataaatga agcatcattt
     2761 tcttttgtcg tttgagcacc actgtgaaca cgaacaatag cacctgaaat attttgtgag
     2821 agggcatcgt agttgatttc ctcaggacgc tgccagccag agaggtcaat aatcggtttg
     2881 tctaagtgtt tcaaagcctg tgcttcaatc tgtgctatat tggattttgt tttaaacgat
     2941 tggctgtcat taagtgggcg attgatgatt aaaatgaaca tcataatccc aaaaaaacta
     3001 aataaaataa gtggatgaat ttgttttctc atatcttata attctaccct aaaaatcaaa
     3061 aaaaatcaaa aaaatgggtt aaggaagaga ctttagagca ttttttcatt caagagtgcg
     3121 gaatgatttg aaatatggta taataaaagg gaatttctac agaaaagaga agattatgtc
     3181 aaattttgcc attattttag cagcgggtaa agggactcgc atgaaatctg atttgccaaa
     3241 agttttgcac aaggttgcgg gtatttctat gttggaacat gttttccgta gtgtgggagc
     3301 tatccaacct gaaaagacag taacagttgt aggacacaag gcagaattgg ttgaggaggt
     3361 cttggctgga cagacagaat ttgtgactca atctgaacag ttgggaactg gtcatgcagt
     3421 tatgatgaca gagcctatct tagaaggttt gtcaggacac accttggtca ttgcaggaga
     3481 tactccttta atcactggtg aaagcttgaa aaacttgatt gatttccata tcaatcataa
     3541 aaatgtggcc actatcttga ctgctgaaac ggataatcct tttggttatg gacgaattgt
     3601 tcgtaatgac aatgctgagg ttcttcgtat tgttgagcag aaggatgcta cagattttga
     3661 aaagcaaatc aaggaaatca acactggaac atacgtcttt gacaacgagc gtttgtttga
     3721 ggctttgaaa aatatcaata ccaataacgc tcaaggcgaa tactatatta cagacgtcat
     3781 tggtattttc cgtgaaactg gtgaaaaagt tggcgcttat actttgaaag attttgatga
     3841 aagtcttggg gtaaatgacc gtgtggcgct tgcgacagct gagtcagtta tgcgtcgtcg
     3901 catcaatcat aaacacatgg tcaacggtgt tagctttgtc aatccagaag caacttatat
     3961 cgatattgat gttgagattg cttcggaagt tcaaatcgaa gccaatgtta tcttgaaagg
     4021 gcaaacgaaa attggtgctg agactgtttt gacaaacggt acttatgtag tggacagcac
     4081 tatcggagca ggagcggtca ttaccaattc tatgattgag gaaagtagtg ttgcagacgg
     4141 tgtgatagtc ggtccttatg ctcacattcg tccaaattca agtctgggtg cccaagttca
     4201 tattggtaac tttgttgagg tgaaaggatc ttcaattggt gagaatacca aggctggtca
     4261 tttgacttat atcggaaact gtgaagtggg aagcaacgtt aatttcggtg ctggaactat
     4321 tacagtcaac tatgacggca aaaacaaata caagacagtc attggaaaca atgtctttgt
     4381 tggttcaaat tcaaccatta ttgcaccagt agaacttggt gacaattccc tcgttggtgc
     4441 tggttcaact attactaaag acgtgccagc agatgctatt gctattggtc gcggtcgtca
     4501 gatcaataaa gacgaatatg caacacgtct tcctcatcat cctaagaacc agtaggagcc
     4561 tatcatggag tttgaagaaa aaacgcttag ccgaaaagaa atctatcaag gaccaatatt
     4621 taaactggtc caagatcagg ttgaattacc agaaggcaag ggaactgccc aacgggattt
     4681 gattttccac aatggggctg tctgtgtttt agcagtaacg gatgaacaaa aacttatctt
     4741 ggtcaagcag taccgcaaag ctatcgaggc tgtctcttac gaaattccag ccggaaaatt
     4801 ggaagtagga gaaaacacag cccctgtggc agctgccctt cgtgaattag aggaagaaac
     4861 agcctataca gggaaattag aactcttgta cgatttttat tcagctattg gcttttgtaa
     4921 tgagaagtta aaactatatt tagcaagcga tttgacaaaa gtggaaaatc cgcgtccgca
     4981 ggatgaggat gaaaccttgg aagtccttga agtgagctta gaagaagcga aagaattaat
     5041 ccaatcaggt catatttgtg atgccaagac aattatggct gttcagtatt gggagttgca
     5101 gaaaaaatag aggaggtcag tatgggtaaa tctttattaa cggatgaaat gattgaaaga
     5161 gctaatagag gcgaaaaaat ttcaggtcct cctttgctag atgataatga ggaaactaag
     5221 attttaccaa cctcttcttc ccgttttggt tatgccaatc ctaaggatca tggttttagc
     5281 caggaaacct tgaagattca ggtcgaacca tctattcata aaagccgtcg tattgaaaat
     5341 accaagagaa atgtcttcaa ttctaagttg aataaaatct tatttgcggt catctttctc
     5401 ttgattttgc ttgttttagc aatgaaactt ttgtaataga aaaggaattg aaatgaaaat
     5461 aggaattatt gctgctatgc cagaagaact ggcttatctg gtccagcatt tagataatgc
     5521 ccaggagcaa gttgttttgg ggaataccta tcatacagga accattgctt ctcatgaagt
     5581 cgttcttgta gaaagtggaa ttggtaaggt catgtctgct atgagtgtgg cgattttggc
     5641 tgatcatttc caggtggatg cccttattaa tacgggttca gctggggcag tagcagaagg
     5701 tatcgctgtt ggggatgtcg tgattgctga caaattagcc tatcatgacg tggatgtcac
     5761 agcttttggc tatgcttatg gacaaatggc gcaacaaccg ctttatttcg aatcagacaa
     5821 aacctttgtt gctcaaatcc aagagagttt atctcaattg gaccaaaact ggcatcttgg
     5881 tttgattgct acaggagata gttttgttgc aggaaatgac aagatagaag cgattaagtc
     5941 ccatttccca gaagttttag ccgtggagat ggagggggca gctattgctc aagcagcgca
     6001 tgccctcaat ctcccagtct tagtcatccg agctatgagt gacaatgcca accatgaagc
     6061 aaacatcttt tttgatgagt ttattatcga agctggacgt cgctctgccc aagtcttgtt
     6121 gacctttttg aaggctttag attaagcgga aatttgacag tttttctagc ttatgataag
     6181 atttaagtaa agaaaagcta gaaaacgttt cagaggatat tatgagtatt gaaatgaccg
     6241 tcagtgagat tgcagaggtc ttaggattat ctcgccaagc aatcaataac cgtgtcaaag
     6301 aattaccaga agaagacaca gataaaaatg acaaaggggt aacagttgtt accagaagtg
     6361 gcttgattaa gctagaagaa atctataaaa aaacgatttt tgaagatgag cctgtcagtg
     6421 aagatgtcaa acaacgtgaa ctgatggaga ttctagtgga tgagaagaat gcagaaattc
     6481 ttcgtcttta tgaacaatta aaagccaagg atcgtcagtt atcagaaaaa gacgagcaga
     6541 tgcgtatcaa agaccgtcag attgctgaga aagacaaaca attagatcag caacaacaat
     6601 tgacccttca agccatgaag gatcaagaaa atcttaagct agagctggac caagcaaaag
     6661 aagaagtcca atccactaag aaaggctttt ttgctcgttt atttggagga taatcaagga
     6721 gctgtttagg ttaaatgcta acctgatggc ttcttttgtt tgtgaatagt atagttgatt
     6781 agaacagaga cagtagagga gaggtacaga atggaacgat tagaagatta cattgctttt
     6841 gacttagaat ttaatcagca cgaaggacaa acacacttga ttcaagtatc ggcagtgcgt
     6901 tttagagatg gtcgggaaat cgatgcctat gattcctatg ttcataccag cgtccctcta
     6961 aaaagcttta tcaatggttt gaccgggatt acagctgaga ccttaaaaga tgctcctcca
     7021 gttgagaaag tcttagaaga attccaagag tttgtgggtt ctttgctgat gataggctac
     7081 aatgctgcta aaagtgattt acctatttta gcagagcatg gtttagacta tagccaacag
     7141 tacaaggtgg atttgtatga tgaagctttt gaacgtcgga gttcagattt acatggtatt
     7201 gccaatctca aattgcaaac tgttgcctca tttcttggat ttcaaggtca gtctcataat
     7261 agtttagagg atgctcggat gacggcgcgt gtttacgaat ccttcttaga gtcggatcaa
     7321 gctagggaat tgttagagac agaaagtagc ttatcaaata atccttttgg tggcttggac
     7381 ttgtctcaat tatttgacta ggagtgccta tgaaacctga aaaacctaaa aaattgggtc
     7441 ttaaacagat ttacctcaat cttgataaaa tcctctttct atttttcttg atttttatga
     7501 tagtggagta tttgtgggta ccattcaatt catggttggc tagcttctta ttagatcaga
     7561 caggttatct ttttatttcc tataacaatg tcttagccat ccttactcat tctccattga
     7621 ttagtctagc ttttttagcc ttgattgtaa ttaactttct ggttgcttat tttcaaatag
     7681 gactcctctt tattggtgct cgtcatctcc tctataatga aaagcgaagg ctaattgagt
     7741 atatccgcaa ggttttccat gaaagttttg aatttatgaa aaagctaact ggcgctaaag
     7801 ccttgtttat ctttttctat atagctatgc tgtttccttt tatacggaaa atgctgaaga
     7861 tttattatct taataaaatc gtcattccag agtttatcca agcttattta gaagataaat
     7921 attggatgtg gtggctgagc atcctacttc tatctcttat ctttctctat gtctctgtca
     7981 gattgatgtt tgcccttccg aagattgtat atgatcagct gactgttcga gaagcagtga
     8041 tgtttagctt ggagaaaacc aagaagagag ttacttttta tgcttggaat ttattttaca
     8101 tcttgctcaa agcacattta ttattttatc ttcctttgat tcctttatta ttggctcaaa
     8161 ctttggtaga tgatatcact cagagagagt ctctgattct tggtattttg aattttgttg
     8221 tgattaaaaa tctctattat atggctctga cttatttttt ggttaaattt gtttcatttt
     8281 tgacaggaaa ggagctggat atgcttccta ggagagaaaa agatcacatc gtgcgatggg
     8341 gtgtcatgac ttgtgccagt cttttctttg ccttagaagg ttatatttat ctggaggctc
     8401 ccatggttca tctacctcaa cttatttctc accgaggggt ttccaatgca aatgggattc
     8461 aaaatacagt agagtccttg gaaactacag cacaactcaa accagacttg gtggagacgg
     8521 acgtgcagga aacaaaagat gggcagtttg tcatgatgca tgatgctaac ttgaaaaatc
     8581 tagcaggtat caataaaagt cctcaagact taaacttgga ggagcttaaa gggattgata
     8641 tttttgaaaa tggctaccag actaaaattt caagctttga agattatctc agtcgagcca
     8701 acgaacttgg tcaaaaatta ctaattgaaa ttaaaaccag taaaaaagat agtccagaca
     8761 tgatgaaccg ctttttagcc cgttatgctg caaagctcaa gatttatgga catcaaatcc
     8821 agtctttaga ctaccatgtt gtcgaaaaag taagacagta tgatgccgaa ctgccagttt
     8881 atttcatcat gccctacaat tctgtctttc ctaaaacaag agcgacagga tatactatgg
     8941 agtactcaac cttggatgaa tattttgtaa gcaaactatg gacaacggat cagaaacttt
     9001 atgtctggac tgtcaatgat tcagaagcta tcagcaaatc tcttcactta ggagtagatg
     9061 gagtgattac agatgatttg gaaaaagttc agcgggaaat agaagtagcc caagaagatc
     9121 cagaatatac ggatttgctc ttgaaaaaag cattggaatt ctttgaattt tagtaataaa
     9181 aaagaggaac ggttgttcct cccatgatag gtaagtattc ttgtgaatgc ttgctttttt
     9241 gtggttgcaa tccgtttacg agaattgttt gtattgttct tgtttcaatt tactttatag
     9301 tgctttgaag ttggaatgct acactataac ttct
//
LOCUS       DAVKGC010000079         9279 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKGC010000079 DAVKGC010000000
VERSION     DAVKGC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.079"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            14..1234
                     /locus_tag="VW049_001546"
     CDS             14..1234
                     /locus_tag="VW049_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9432636.1"
                     /translation="MLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILYEKDATQP
                     VEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPMEARNYTVE
                     ELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTTGLNNETL
                     GDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTITSTNYML
                     EGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPYARFTATS
                     SLMDYISSTFTLRKIVQQGDAYQDSKTPVQDGKEDTVTAVAPEDIYLIERVGNQSSQS
                     VQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKIEKAFFLK
                     VWWNQFVRFVNEKL"
     gene            complement(1465..2877)
                     /gene="sufB"
                     /locus_tag="VW049_001547"
     CDS             complement(1465..2877)
                     /gene="sufB"
                     /locus_tag="VW049_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9432637.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(2931..3371)
                     /locus_tag="VW049_001548"
     CDS             complement(2931..3371)
                     /locus_tag="VW049_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9432638.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(3358..4584)
                     /locus_tag="VW049_001549"
     CDS             complement(3358..4584)
                     /locus_tag="VW049_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9432639.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVINPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(4595..5857)
                     /gene="sufD"
                     /locus_tag="VW049_001550"
     CDS             complement(4595..5857)
                     /gene="sufD"
                     /locus_tag="VW049_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9432640.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(5885..6655)
                     /gene="sufC"
                     /locus_tag="VW049_001551"
     CDS             complement(5885..6655)
                     /gene="sufC"
                     /locus_tag="VW049_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9432641.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(6805..6999)
                     /locus_tag="VW049_001552"
     CDS             complement(6805..6999)
                     /locus_tag="VW049_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9432642.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(7027..8682)
                     /gene="dnaX"
                     /locus_tag="VW049_001553"
     CDS             complement(7027..8682)
                     /gene="dnaX"
                     /locus_tag="VW049_001553"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9432643.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(8682..9179)
                     /locus_tag="VW049_001554"
     CDS             complement(8682..9179)
                     /locus_tag="VW049_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9432644.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
BASE COUNT         2709 a         2076 c         1747 g         2747 t
ORIGIN      
        1 aatattttta actttgttaa ctgtctctct tttagggggt gcttctactg ctgttgctca
       61 agattttacc attgccgcta aacatgcgat tgctgttgag gcaaatactg gtaaaattct
      121 ctatgagaag gatgcaacgc aacctgtcga aattgcttcc ataacaaaat tgattactgt
      181 ttatctggtc tatgaagctt tggaaaacgg aagtattacc ctctccactc ctgtagatat
      241 ttctgattat ccttatcaat tgacgacaaa ttctgaagcc agtaatattc ctatggaggc
      301 ccgtaattat actgtcgaag agttgcttga agcaactctg gtatctagtg ccaacagcgc
      361 cgctattgcc ctagctgaga aaattgctgg ctcagaaaaa gatttcgtcg atatgatgcg
      421 ggcaaaactc ttggaatggg gaattcagga tgccactgtt gtcaatacga caggtcttaa
      481 caatgaaact ctaggggata acatttaccc aggttctaaa aaagatgagg aaaataagct
      541 tagtgcttat gatgtcgcta tcgttgctcg caacctcatc aaaaaatacc cacaagtctt
      601 agaaatcacc aaaaaacctt cttctacttt tgctgggatg acaatcactt caaccaacta
      661 catgttagaa ggtatgcctg cttaccgtgg tggttttgat gggctaaaaa caggaacaac
      721 agataaggct ggagagtctt ttgttggtac tactgtcgaa aaaggcatga gagtcatcac
      781 agttgtttta aatgcagatc atcaagacaa taatccttac gctcgattta cagctacatc
      841 ttccctaatg gattatattt cttctacatt tacacttcgc aaaatcgttc aacaaggcga
      901 tgcctatcaa gatagcaaaa cccctgtaca agatggaaaa gaagatacag taactgcagt
      961 ggctccagag gatatctatc taatcgaacg tgttgggaat caatcttccc aatctgttca
     1021 attcacacct gattccaaag caatcccagc accacttgaa gctggaacag tggttggcca
     1081 tttgacttat gaagacaagg acttgattgg tcaaggttac atcaccacag agcgccctag
     1141 tttcgaaatg gtagcagaca agaaaattga aaaagccttc ttcttaaaag tttggtggaa
     1201 tcagtttgtc cgctttgtta acgagaaatt ataaaaaatc gcgattttaa accgcgattt
     1261 tttgatacaa gttcatcttc aaaatccttg attttgagta aatctaatac tcaaaatcaa
     1321 agaacaaact aggaagctag ccgcagcttg ctcaaaacac tgttttgagg ttgtagatga
     1381 aactgacgaa gtcagtaacc atacatacgg taaggcgatg ctgacgtggt ttgaagagat
     1441 tttcgaagag tataaaatca aattttatcc aactgatccc tccatttcat agctaatcaa
     1501 gcggttcagc tcaactgcgt attccattgg aagttctttt gtaaagggtt ctacaaatcc
     1561 catgacaatc atttcagttg cctcagattc tgacaatcca cggctcatga gataatacaa
     1621 ttgctcttct gaaatcttag atactttggc ttcgtgttcc aaagccactt gcgagttgtg
     1681 aatttcatta aatggaatag tatctgatgc tgacaagtca tccatgataa tggtatcaca
     1741 ttcaatgtgg gaaacagatt tcttagagtt cttgttaaag gtgacttgtc cacggtagtc
     1801 aacctttcct ccacctttag cgatggattt agacacaata gacgagctgg tatgtggagc
     1861 attgtgaatc atcttagcac ccgtgtcttg gtgttgccct gcattagcaa aggcgataga
     1921 gagcatggta ccacgcgctc cttctccatc aaggtaaaca gatggatatt tcatagtcgt
     1981 tttggcaccc aagtttccat caatccactc aacagtggca tccttttgag ccttagcacg
     2041 ctttgttacc aagttataga cgttatcaga ccagttttgg atagttgtat aacgcatata
     2101 agctccgtcc aaagcaaaaa tttctacaat ggcagcgtgt aagctattgc ttgaatatgt
     2161 tggtgctgta catccttcta cgtagtggac gcttgctccc tcatcaacga taatcaaggt
     2221 acgttcgaac tgacctatat tttcgttatt gatacggaaa taagtttgaa gtggaatatc
     2281 taccttgaca ccttttggca cgtagataaa agttccaccc gaccatactg ctgagttgag
     2341 ggctgccaac ttgttatctg tcggcggtac caacttcgca aagtattgtt taaataagtc
     2401 tgggtattcc ttgagtgcgg aatctgtatc tgtaaagata atacctaatt tttggaactc
     2461 ttccttcatg ttgtggtaaa ccacttctga ctcgtactgg gcagaagccc ctgctaaata
     2521 agcacgttca gcttctggaa tcccgatacg ttcaaaggtt tctttaatct tttcaggtac
     2581 atcatcccaa gaacgggctg gtttgtcaga tggtttttgg tagtagatta agtcatcaaa
     2641 gtcaatctct gacaagtctg ctccccaagt ttgcatgggc atttttttga aggtttcata
     2701 agacttcaaa cggaactcca acatccactc aggctcaccc ttagcagcag ataattcacg
     2761 aataacacct tcgttgagtc cttttcctgt cgataagaca ggctctacat cgtcatggaa
     2821 accaaattta tattcaccaa ggtcaattgg ttttggttct actctttctt cagccatagt
     2881 atcctttctt tcttcatttc taataattca taagacaaaa gaaacttgtc ttactgtttt
     2941 tcttgatttt caattgtttt cttaagggca ttccaagcta gggttgcaca cttgattctt
     3001 tgagggaatt tggcaacacc tgacaagaat gccgcgtctc caagttggcc ttgacgctca
     3061 tctttttgcc cttgaaccat ttcagaaaaa atagtcgcca gttctaaaat ttcttgtttg
     3121 gtttttccta aaacggcatc tgtcatcata ctagcagaag cagttgaaat cgtgcatcct
     3181 gaatttagaa aagcaatatc ttccaaacgg tcctctgcat caaacttgac agagaggttg
     3241 atgacatccc cacaagtcgg attgttgaga ctgatttgct cagcatcttc taacttccct
     3301 tggtgatgtg gatttttcga atggtctgct accactgcca tataaaggct atctagttta
     3361 gaaagtgcca ttgaaaaact cctttgtctt ttgtagggca tcgactagtt tgtcgcaatc
     3421 tgccttggta ttgtagatat aaaaacttgc acgagctgtt gctgggactt ccaaatactg
     3481 aagcaagggt tgcgcacaat ggtgaccagc acgaacagcc actccttcat aatccagagc
     3541 cgtcgcaaga tcgtgaggat ggagatcacc taggttaaag gcaataacac ccgaacgttg
     3601 agccaaatcc tgagaaccgt aaatggtcaa tccctcaatt gcctgcagtt ttggatagac
     3661 gtacgcaatc aattcctgtt catgagcttc aacggcatcc ataccaatct tttccaaata
     3721 atcaactgca gtcgcaagtc caatagctcc tgccatattt ggcgttccag cctcaaattt
     3781 ccaaggcaat tccttccaac tagcaaattg ctcgtagaca aaatcaatca tctcgccgcc
     3841 aaattctact ggagacattt gctcaagata cttttctttg ccgtaaagga caccgatacc
     3901 agtcggacca gccatcttgt gacccgaaaa ggcgaaaaag tccagatcca agtcctggac
     3961 atcaatcttc atatgaggtg tagattgagc accatccact accatgattg ccccaacttg
     4021 gtgggctaat tgagtgattt ccttgatcgg attgatcaca ccaagaacat tggaggcatg
     4081 agctagggaa acaaatttaa ccttatcagt caatttagct cgcaaatcct ccatatccaa
     4141 ggcaccgtct ttaagataga cataaacaag ctctgctcca gtctttcgac aagcttcctg
     4201 ccatggaatg atattagaat ggtgttccat tactgaaatc aagacctggt ctccctcagt
     4261 gagaatttcc tcagcaaagc gtgccaccca gttaaggctg gttgtcgttc ctctggtaaa
     4321 gagaacttcc tttgtagagc ctgcattaat aaacttacga atggtttcac gagcagcttc
     4381 ataagaagct gtcgctcgtt ccgctaaggt atggacacca cggtgaacat tggcattgtc
     4441 ctgctcatag taactgttaa tagctttcag aactactagg ggtttttgtg tcgtcgcagc
     4501 attgtccaga tagaccagag gttcatcatt gacaatctga tctaaaattg gaaaatcctt
     4561 gcgaatcgct tctacatcta acataggctt ccccttagcg ttttgacaat ttctcttcga
     4621 tagttgcaat catttcatca cgaacttcct tgactggaat ctccacgata acagatccaa
     4681 ggaaaccacg aacaaccaaa cgctctgcag ttgccttatc caatccacga ctcatgaggt
     4741 aatacatgtc ttctggatca acttgtccga tagacgctgc gtgtcctgca gtgacatcat
     4801 tttcatcaat caaaagaatt gggttagcat ctgaacgcgc ttggtctgaa agcatgagaa
     4861 cacggctctc ttgttgcgca tctgctccct tagcaccctt gatgatgtgg ccgataccat
     4921 tgaaagtcaa agttgctttt tcaaggataa ccccatgttg taggatattt ccgatagagt
     4981 tgcagccata gttagttaca cgagtatcaa tcccttgtac ctgacgacca cttgaaagag
     5041 ctacaacctt gaggtcagca tggctaccat taccaatcaa gtcactatca aaatcagcaa
     5101 cgacatttcc ttcgttcatg acaccgatag cccagtcaat acttgcatcg ttgcctaatt
     5161 taccacgacg gctaatgtag gcagtgacgt tttcacctag acggtcgata gcagcaaact
     5221 tgacttgcgc accagaacgt gcaatcactt ccactgtgat attggcagtt actttgtcac
     5281 ttccttcacc gcgtgactct aaacgctcca gataactaat cttagaattt ttaccagcga
     5341 taatcataat atgcttgtta aacggcacat tgctatcgct atcttggtag aaaattcctt
     5401 caattggctc tgtgatttct acgttatctg gaatatagag tacagcacca ctgttaaagt
     5461 aagctgtgtg gtaagccgcc aacttgtcat catcatactt aacagatgac atgaagaatt
     5521 cttcgatcag ctctggaatt tcttctaaag ctgagtgaaa gtctgtgaag acaacaccct
     5581 gttcagctaa ctcaactgga gtttgttcga aaacagtttg agttcctact tgcaccaact
     5641 tcaagtgatg atctaaagct gtgaaatctg gaacatttgc tgatggctca ttttctgtaa
     5701 tcgttccatc acccagattc caacggtgga atttgacacg ctcaataact ggtaattcca
     5761 aagtctcaat cttgtcaaaa gctttttgac ggagatcagc caaccagctt ggttcagcgt
     5821 gcatttctga aaaaagttta atattttctc tagtcattta cttctcctaa aagatacgag
     5881 gaaattacaa ttcttccttg tagtcgtagc caagttcttc agctaatttt gcgtatcctt
     5941 cacgttccaa acgcgcagcc aattctggac caccagaaag gacaacacga ccttccatca
     6001 tcacgtgtac cacatcaggt gtgatatagt tcaaaagacg ttggtagtga gtgatgatca
     6061 tagcaccaaa accttcacca cgcatggcat tgacaccttt agacacaact ttaagagcgt
     6121 caatatcaag acctgagtca atctcgtcca aaagagcaaa tgttggctcc aacatcaaaa
     6181 gttgaagaat ttcattgcgt tttttctcac caccagagaa gccttcgttg aggtaacgct
     6241 ctgccatttc ttctttcatg ttgagcaatt ccattttttc atctagctta gtaataaact
     6301 cacgaactga aatcttctca tcatcttctt tacccgcatt catagcggca cgaagaaact
     6361 cagcattggt aattccaggg atttctgatg ggtattgcat agcaaggaaa agtcccatac
     6421 gcgcacgctc atccacttcc aactcaagga tgtttacgcc atcaaacaaa acttcacctt
     6481 tagttacttc atagtttgga tttcccatga tagcggcaga aagagtcgat ttacctgtac
     6541 catttggtcc catgatagcg gcaatttctc ctgttttcag ggtcaggtta acccctttta
     6601 aaatttcttt tccttcaatc tcaacgtgaa gatctttaat ctctaatact gacatgatag
     6661 ttcctttctt tttcaaaact tttacagtac ttactagaaa aacgtctgat ttccccagaa
     6721 atagagcaaa aattctataa atatactttt ctagtataac aaaaaaaagc ctaaaaggct
     6781 ttgattttgt ctggaagctg agggctagtc tttcctatta aaatgctgat cgatgacatc
     6841 aacgattttg cccatcacat aactgactcg gaaatttcta aagagtaaaa ttgcccaaaa
     6901 ggctgccata atatagcggc cagtcatcca ggcttcattg aaaaaatagg tctcagcagc
     6961 tgtaaacagc gccataataa taaattgttg tttattcata aacttattgt atcatgaaat
     7021 ctttctttag tcttcctcta ccttcacttt atcagccaaa aattcaaatc cttctggaat
     7081 caggctttct tctacttctt tttcagtttg agaagatttg gctttggctg aaaaggctgc
     7141 gcgaacttct ttccattcct ccatggaaat agctaaaatc tcaggtgaaa aacctgccgc
     7201 ctgactgagg atattaccaa acatggtatt gagattgtct cgtttcatag tttgaccagc
     7261 attgaagtta gactcaaaag caagaatagc atggtgttca ttggcagcaa ccggttgaga
     7321 accaactagc agagccttgt ccggcccacc tagactttca attacctctc cccaggcatt
     7381 ctgcaaacga attagatttt gacgtgctaa atcaggattt tcgacggcct cttgtaagat
     7441 agattgcact ttattgcgat cgacacgata gactgttttg cccgtagctg gtcgactagg
     7501 agctggtgca acttgtttag gaaccgcacc tacattagaa agctcttgtt tgagacgggc
     7561 aacttcctgt ctcagcgtag caatttcatt ttcaaccgct cctgatagag ctggttcggg
     7621 cttgatttcc gccaaacgga cggtcatcat ttcagcataa atcttgggct gcaaactaga
     7681 cttaatatct gctaaactca ctgttgctaa gcgaatcatt tcaaacagat ttttttgagg
     7741 aagtgccaaa ttttctacaa agactgaact atgatgagta ttttctcccc ctgtttgaac
     7801 aattaacaag tctcttaaat agtgcaaaag atcggtcaca aaacgagtca tgctcttacc
     7861 attgtcaaaa agaagattca agcaagacaa agctttggga acatcctgtt gagacaaggc
     7921 cgccacataa tcatccaagg ctgataggct aatggtgcca gtaatttctt cagagatagc
     7981 agtcgtcagc tcatttccct gtgtcaaact cagggcttga tccaaaatag acaaggcgtc
     8041 ccgcattcca ccttccgccc gtctggcaat gatttccaca gcctctggtt cagaactgat
     8101 attttctttt tctaagatat agtgaatatg ttccttaata tcctgtgtct taattgattt
     8161 aaactcaaaa cgttgcacac gggatagaat agtagcagga atcttgtgca attcagtagt
     8221 ggccaaaata aagactacat tctgtgttgg ttcttccagc gtctttagga gggcattaaa
     8281 agcccctgta gacagcatgt gaacctcatc tatgatataa accttataac gagcaaggct
     8341 aggcgcatag gtagatttat cacgaatttc gcgaatttca tctaccccat tattagaagc
     8401 tgcatccatt tcaatgacat cttctaaact accgtccgtc actgcttgac aaatatagca
     8461 gttattgcaa ggttcgccac ccacttgatt gggacagttc atagccttgg caaagatttt
     8521 agcaacactg gtttttcccg ttccacgagg accagaaaaa agataagcgt gacttatttt
     8581 ctcttgctcc accgcttgtt taagagtctt agccacaact tcttgaccaa ctaactggga
     8641 gaagttttga cttctatatt ttcgataaag tgcttgatac attaagattt ttcctcaaac
     8701 atcgtaaagt cccatgttgt cttttcaagc aaaatagcga caaattgttc caaataatct
     8761 cgatccatag catcgtaatc ctcaatctct gaagaatcca gatccagaac tccaagtaac
     8821 tgaccattct tcatcatcgg caccacaatt tcacttttag ctagactatc acaagaaata
     8881 tagttgagat aggtcgtcac atctccaaca ataacagttt cctgaaagtg agctgcctca
     8941 ccacaaaccc ccttgcctag tgcaatacgg atgcaggaaa cacctccttg gaaggggcct
     9001 aaaaccaatt cctttccatc gaacaaataa aagcctgcaa atacggtatt aggaaaacgt
     9061 gattttatga gagcactggc gttggaaaga ttagccaaaa cattggtttc gccttccaat
     9121 aggaaggaca attcttcatt taacatttga taacgtgatt gtttttctga ttttaacata
     9181 gaactattat atcaaaaaag gagcagagac aaaagaaaaa tcccttgttt agtaaacaaa
     9241 ggattttgga ctctataata tttgtagtgg gtaaatcca
//
LOCUS       DAVKGC010000080         9275 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKGC010000080 DAVKGC010000000
VERSION     DAVKGC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9275
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.080"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(73..312)
                     /locus_tag="VW049_001555"
     CDS             complement(73..312)
                     /locus_tag="VW049_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9432645.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(332..604)
                     /gene="rpsP"
                     /locus_tag="VW049_001556"
     CDS             complement(332..604)
                     /gene="rpsP"
                     /locus_tag="VW049_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9432646.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            774..971
                     /locus_tag="VW049_001557"
     CDS             774..971
                     /locus_tag="VW049_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432647.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKRNMDLLADD
                     SLAGDIILLWRIQFGTFTTET"
     gene            1450..1698
                     /locus_tag="VW049_001558"
     CDS             1450..1698
                     /locus_tag="VW049_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432648.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEEVEEVEEVEDFQDDDEIPTII"
     gene            1781..2584
                     /locus_tag="VW049_001559"
     CDS             1781..2584
                     /locus_tag="VW049_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9432649.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(2640..4181)
                     /locus_tag="VW049_001560"
     CDS             complement(2640..4181)
                     /locus_tag="VW049_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9432650.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(4183..4665)
                     /locus_tag="VW049_001561"
     CDS             complement(4183..4665)
                     /locus_tag="VW049_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9432651.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            complement(4665..5750)
                     /gene="rlmN"
                     /locus_tag="VW049_001562"
     CDS             complement(4665..5750)
                     /gene="rlmN"
                     /locus_tag="VW049_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU9432652.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(5771..6301)
                     /locus_tag="VW049_001563"
     CDS             complement(5771..6301)
                     /locus_tag="VW049_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9432653.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(6457..7062)
                     /locus_tag="VW049_001564"
     CDS             complement(6457..7062)
                     /locus_tag="VW049_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9432654.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(7235..8272)
                     /locus_tag="VW049_001565"
     CDS             complement(7235..8272)
                     /locus_tag="VW049_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9432655.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(8306..9241)
                     /locus_tag="VW049_001566"
     CDS             complement(8306..9241)
                     /locus_tag="VW049_001566"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9432656.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
BASE COUNT         2754 a         1991 c         1729 g         2801 t
ORIGIN      
        1 tgtcctgtta ttatttcatt ttactatatc ttctattcca cacaaaaaag cgagacatcc
       61 gtcccgccct tcttattttt cgtcaataac gattcttact tttttgtatt cagttgggac
      121 agagtagaca atcgttctta tcgcagaaat agtgcgaccc ttacgaccga ttacacgacc
      181 cacatcgctt tgatcaagat tcaaatgata ttccaaaaat tctggtgtat cctcaatctt
      241 gatagttaag gcatctggtt gtgaaattaa gggtttcaca atcgcaataa tgagattttc
      301 aatcgtatcc atctgtcaac ctactttaaa cttattttga gaatttagaa tcgtggaatt
      361 ttttcaatac gccttctttt gaaaggatgt tacgtactgt gtctgaaggt tgagctccat
      421 tagccaacca tgcaagaacg cggtcttctt tcaaagttac ttggttttca gcaacaagtg
      481 ggttgtaagt tccaactgtt tcgatgaaac gtccgtcacg tggtgaacgt gaatctgcta
      541 cgttgatacg gtagaaaggt tttttcttag aacccatacg agtcaaacgg attttaactg
      601 ccatttttaa agtctcattt ctttaatttt ttatttcggt gaaatagctg agctatttag
      661 cacatgttct attatagcag atttctggca ggtgtcaaga aaaaaacttg acagactata
      721 aatttcttaa agcatttaga aatttgttag aataggaaat aaatgtttga aagatgctat
      781 ccgtgaatac tattttagaa aaattttata aagaacacca agtcaaaccc ttcatatcgc
      841 ctgaacgaga gttggatact tggcttctaa gtcccaagcg aaatatggac ttattagcag
      901 atgattcact agcaggagat atcattttac tatggagaat ccagtttggt acttttacca
      961 ctgaaacatg attttaaaaa ctcccattcg aatatgattc aaaattcgct tagaatcaat
     1021 gttttcccac tttttcaaaa aataaatttt catctaattt aaataacttt caattaagtg
     1081 tgaattttgc ttaaaatcat taaaaaacac cccatggtgt gaaccatgaa gtgttgtaaa
     1141 tgctgtgttg tagctagttc ttagcgttta gagaactggc tagctttacg agctttctta
     1201 agatctggtt tccaaaatac tttgagtaca cttatggtat tgatgcccct aaacatctaa
     1261 aaactctggt agaaaaaggg tatgctcttg tcgaaacttc ttttgactca cttgaccatc
     1321 taaataatac catgaaaaag aatatcctga agaagaaggg agttactgga ctttctaaaa
     1381 tgaaatcttg cgacctggat caagcccttc atgagcattt ttcagaagaa gaattagctg
     1441 gtcactttca tgtccttcta tggacttttt tacaatggca ttgctatcac acccaatacc
     1501 tatctaagcg cctggttcgt aaactttatt gcagctcttc ctctaaattt cctaattgtt
     1561 gaaccaattg cccgttttat actaagttct tttcagaaac catttactgg ggaagaagtt
     1621 gaagaagttg aagaagttga agaagttgaa gaagttgaag attttcaaga tgatgatgaa
     1681 atcccaacta ttatctaagc cagttctgta aactactaat atttgaaatc cacttccttt
     1741 tagggtacaa tggtataaat gaatttttga gaggatcaga atgaaaaaac tagcaaccct
     1801 tcttttactg tctactgtag ccctagctgg gtgtagcagc gtccaacgca gtctgcgtgg
     1861 tgatgattat gttgattcca gtcttgctgc tgaagaaagt tccaaagtag ctgcccaatc
     1921 tgccaaggag ttaaacgatg ctttaacaaa cgaaaacgcc aatttcccac aactatctaa
     1981 ggaagttgct gaagatgaag ccgaagtgat tctccacaca agccaaggtg atattcgcat
     2041 taaactcttc cctaaactcg ctcctctagc ggttgaaaat ttcctcactc acgccaaaga
     2101 aggctactat aacggtatta ccttccaccg tgtcatcgat ggctttatgg tccaaactgg
     2161 agatccaaaa ggggacggta caggtggtca gtccatatgg catgacaagg ataagactaa
     2221 agacaaagga actggtttca agaacgagat tactccttat ttgtataaca tccgtggtgc
     2281 tcttgctatg gctaatactg gtcaaccaaa caccaatggc agccagttct tcatcaacca
     2341 aaactctaca gatacctctt ctaaactccc tacaagcaag tatccacaga aaattatcga
     2401 agcctacaaa gaaggtggaa accctagtct agatggcaaa cacccagtct ttggtcaagt
     2461 gattgacggt atggatgttg tggataagat tgctaaagcc gaaaaagatg aaaaagacaa
     2521 gccaactact gctatcacaa tcgacagcat cgaagtggtg aaagactacg attttaaatc
     2581 ttaaaaacca aaaaaataca gtatccacat tcggtactgt atttctttta ctctcattct
     2641 taagttaaat tattaaaatc ccatatttgg tctatccaac cttcataaaa gtctggctcg
     2701 tggcagacca taaggataga tcccctgtat tctttgagag cgcgtttgag ctcatccttt
     2761 gcatccacat ccaaatggtt ggtcggctcg tccagcacta aaacgttgtt ttcacgattc
     2821 atcaagagac agaaacgaac cttggcttgc tctccccctg ataatacttg aatctggctt
     2881 tcaatatgtt tggttgtcaa accacaacgg gcaagggctg cacggacttc tgcttgatta
     2941 agggcaggaa aggcattcca gacagcttca agaggagttt ggcgattacc gccttctact
     3001 tcctgctcaa aataaccaag ttctaaataa tctccacgct ccacttcccc agcgattggc
     3061 gagataatgc ctaagagact cttcaagaga gttgtttttc caataccatt agcaccaata
     3121 atcgcaacct tttgattgcg ttcgaaggta agatttaaag gcttagtaag aggacggtcg
     3181 taaccaattt gcaagttctt ggcttggaag ataaagcgcc ctggtgtacg agctggtttg
     3241 aaatcaaagg atggttttgg tttctcactt tggagttcga taatatccat cttatccaat
     3301 ttcttttgac gagacatagc catattacga gttgcaacac gggctttatt acgagccaca
     3361 aagtccttga ggtctgcaat ctctttctgc tggcgttcgt aggctgcctc tagctgagat
     3421 ttcttcatag cataaacttc ttggaactgg tagtagtcac cagagtaacg cgtcagctgt
     3481 tgattttcca catgatagac aatattaata acgtcattga ggaatggaat atcgtgcgaa
     3541 ataagaacaa aggcattctc atagttttgg agatagcgct tgagccaatc aatatgctca
     3601 gcatccaagt agttggtcgg ctcgtccaac agcaaaatat caggcttttc aaggagaagt
     3661 tttgccaaaa gcaccttggt tctttgccca cctgacaaag aagttacatc cgtatccatc
     3721 ccaaagtcca taacaccaag agcacgcgct acttcgtcaa tcttagcatc caaggtatag
     3781 aaatcacgac tctccagacg gtcttgaagt tctcctactt cttccatgag agcatcaaca
     3841 tccgcgccgt cttcagccat tttcatatag aggtcattga tacgagcttc agctttgaaa
     3901 agctcatcaa aagccgtacg gagaacatca cgcaccgact gtctttcagc aaggacagag
     3961 tgctgatcca agtaaccagc cgtcacatat ttggaccact caacctttcc ttcatctggc
     4021 agcattttac cagtcacgat actcataaag gttgattttc cttcaccatt ggcaccgacc
     4081 aggccgatat gttctccctt gaggagacgg aaggacacat cttcaaaaat tgcacggtca
     4141 ccaaaaccgt gactcagatt tttaacttct aaaatactca ttttaattcc ttaccttgtt
     4201 tttatgtaat cgtttataaa ggagccaagc cagatagcca cccaaagtgt tggtccacaa
     4261 atcatcaatc tcaaagacgc gattgaaatc aaagaaaaag tccaagatta attgcgtaca
     4321 ctcgattcca agactcacaa gaaaactaaa aagaaggacc ttttttgttt tccgcaaatt
     4381 tggaaataga taaaggagtt ggaaaatcag aggaaaaaac aagaagacat tgaggatatt
     4441 ttgtaaaaaa atccaacata attgtccaat gtcactcact tcgcccagtt tccagagaga
     4501 attgaaagga gtcaaaagaa aaaccaggcg tccaagatgc tgaatacctg gagttcccac
     4561 tcccacggta gattgttctt gaggagtaaa gcaaaaacag acaatgcaaa tgctatagaa
     4621 aatgactccc cagaccaaaa tatgattata agtcttcttc atcattaagg atttaccgct
     4681 gcgactgcct tctggcggtc acgtttcatt gtgttagagc gcaattgtcc acaagctgcg
     4741 tcaatatctg taccatgctc ttgacgaacc acacagttga cccctttttt cttaagcgta
     4801 tcatagaaag ccaacacgcg ctctttggga ctacggctat attggtcatg ctcactaact
     4861 gggttataag gaatcaagtt tacataagac aatttcttga tgttcttgag caattcagcc
     4921 aattccaagg cttgttctac accgtcgttg acttcattaa gcatgatata ttcaaaggtt
     4981 acacgacggt ttgttgtctc aatatagtat tcaatagcag caaagagttt ttcaatcgga
     5041 aaggcacggt taattttcat gatacttgaa cgaagttcat tgttgggtgc gtgaagagac
     5101 acggcaagat tgacctgaac cccttcatca gcaaagtcac gaattttatg agccaaacct
     5161 gaggttgaaa ccgtgatgtg acgagcaccg atagccattc ctttatcatc attgatagta
     5221 cgaaagaaat tcaagacatt gttgtaatta tcaaagggct caccgattcc catgacaacg
     5281 atatggctga tgcgttcatc ctgaccacgc tcatcaaagt atttctgaac cagcatgatt
     5341 tgcgctacga tttcaccgtt attgaggtca cgttgcttct taatcaaacc agaggcacag
     5401 aaggtacaac cgatattaca gccgacctga gtggtcacac agacagataa accatagtgt
     5461 tgacgcatga gtacagtctc aattaacata ccgtcgggca attcaaagag atatttgact
     5521 gtaccatcag cagactcttg cacaatacgt tgtttcaagg gattgaccac aaactggtca
     5581 ttgagcttag caatcaaatc cttggaaagg ttggtcattt cttcaaatga ctgcacacgt
     5641 ttacggtaga gccattccca gatttgatct gcacggaatt tcttttctcc ctgctccaat
     5701 acccattcct gcatggtttg atgtaccaaa ctatgaattg agggtttcat ttcttctcct
     5761 tattctctac tcacttctga cgaatgacaa aatgacgttg tcccttgtcc tctttctgag
     5821 aatgctggtc tttctgacga cgtctatttt tcttatctgc attcgacttt cgtttagttt
     5881 gagtcggttt ctttcctttt ctagaaggtg tttcttcttc cgtcttacgc attttcttgt
     5941 caaatgatgc tcgcttaggg gcttcatttt ctaagacaaa ataggcacaa ccataactac
     6001 aatactctaa aaggtagtct tgtaaacgac tgattttttc aagtttttct tctgttcggt
     6061 catccttgta aaaacctcgt aggcgaagct gttcgttgct ccagtccccc acgatataat
     6121 caaacttggt taagacttct gaaaaacgct gattaaaagt cgtcacatca aaggcatcct
     6181 tgatattttc aaccaaggaa aaagctatcc cttccgtttc gaccttgtcc ccgtgtaaat
     6241 ggaactccgg accaggaaac ttgttatagt tgtataattc aggtgcaatt tcttttcgca
     6301 tagatatcct ttttccacga ttacttaaca ctttattcta ccataatttc tagcagttag
     6361 cacgtttctc ataaaaatga aaaaagtctg acgattttgt cagaccagaa tcttataacc
     6421 taaaaagaga agaacaattc ttccctccaa ctatcattat ttagcagctg cgtacaattc
     6481 atctacttta ttccagttga ttactgaaaa gaaagctttg atgtagtcag gacgcacgtt
     6541 gcggtatttc acgtagtaag catgttccca aacgtccaag cccaagattg gttttttacc
     6601 ttctgagatt ggtgtgtctt ggtttgctgt tgaagtcact tcaagtttcc cttctttgtt
     6661 gacaaccaac catgcccaac ctgaaccaaa acgagttgtt gctgctgcag tgaaggctgc
     6721 ttggaattct tcaaatgaac caaatgttgc atcgattgct gctgccagtt ctgctgaagg
     6781 agctgttttc tcgggagtca tcaattccca gaaaagagcg tggttcaagt gtccgccacc
     6841 attgttgata agtgcttgac ggatatcagc tgggatagat tctacatcag caagcaaggc
     6901 ttcaaggtct tcaccgattt cagggtgttt ttctaaagct gcattggcat tgttgacata
     6961 agtttgatgg tgtttgtcat ggtgtaaatg cattgtttcc gcatcgatgt atggttccaa
     7021 agcgtcgtat gcatatggaa gttctggtaa gataatagcc atctgtaata cctctttttc
     7081 tttctatatg aaaatgataa cgcaaacatt cacttttttc aagttttttg cttatagata
     7141 aggaaatcac tgacatactt tctaataaaa taagcaaatc agcaagaaac ccacgacaag
     7201 ataatcctga cgcgggttgt agtttcaaca aatgtcaatt gacctgacta gcaatctgta
     7261 agagtgcctt ttcaaaaagg aaaccttttt cataaagacc tgtcttaatc tgatagtctg
     7321 tctcaatcaa ataggaaata gcttgcttca gaaagctcaa agaaagtcct ctcgaatctc
     7381 ttaatgcaaa cttgatttga taaggatttg ggttacgtcc cagataacta cctaaactac
     7441 ttgcaatctg cgattctgtt tggccagact ccgccaaaat cttcacctga gtaaaagtcc
     7501 gaaattgtcc cagcatgact gcaatcagtt tgatttcatc ttccccttgc aaggtcaagt
     7561 ctctcaccaa atcgcgcgcc tgatccatct ttttagtcag aataaactga gttaaatcaa
     7621 aaatattgtc ctgcaaggtc ttgggaattg cgttaacaat atcctcttcc tcgataacag
     7681 aatccgcctt ataggactgt aaaaagagaa gatttttctg gatttcgcta aattgaaacc
     7741 ccgacttgat gaggagattt tcaaaagaat gattggtaaa ctgcagacct tgtttctgac
     7801 tccacttttg gaaatactgg cgcaattctt gttcttttac ttctactgca tcgaagacct
     7861 tggcatcacg cttaagtaat ttgactaacc gtcttttgct atccagcttt ccttctgcaa
     7921 agattatcaa cttggttgtt ggagaaggat tgtcaaggta ttcctcaaat gacttaagct
     7981 catcatctgt caaaaagcgt ttcttagcag tcgtgatatc cataaaataa tctaatatca
     8041 ctattttctc atccgcaaag aaaggaaggc tgaccaactc cagttccaca tccttgtaaa
     8101 ctacttcttt catatcaaag taggcaaagt tgaggtcagc agaatcatac ccaatctgtt
     8161 tcaacatttg actcttcatc acttcaaact gaccctgatc tgtccctgta aataggctca
     8221 ggctcggtaa atttgataaa gtcaacttct gactttcttc aatggctagc atcgactctc
     8281 ctttcttcag atttttcgat ttaatttagt caatatagcg caatttccca cggaaatctt
     8341 ctaagctctc gtagcctttt tccaccatga ttgctttcag ttcattggta atgcggtcaa
     8401 aagcactgac gccttctttg tgaagggtcg ttcccacctg caccatactt gctccacaga
     8461 ggatgtgttc aaaggcatct cgaccagtca gaacgccacc tgttccgata atttggattt
     8521 gaggatttaa acgttgataa aaggcgtgaa cattggctag agcagtcggt ttgatgtatt
     8581 ctccaccaat tccaccaaaa ccattcttag gccgaataac gacagattcg tcttctatat
     8641 agaggccgtt tccgatagag ttaacgcagt tgacaaactt gagcggatat ttgttgaaaa
     8701 tagctgccgc ttggtcaaag tgaacaatat caaaataagg tggcaattta attccaagag
     8761 gtttggtgaa gtaagcaaac acttctgcca aaatccggtc tgttgtctca aaatcatagg
     8821 caatctgagg tttacctgga acatttggac aggaaagatt tagctcagtc agaccacgaa
     8881 aatcactctc ttggactttt ttcaaaatag tatgggtttc ctctggagac atgccgacca
     8941 gagataagaa gaaagttcgg ttcgactctt tttcctgcaa atctaaaaga taatccaaat
     9001 aatagtctaa gccattattt ggcaagccca tagagttgat ggaaccaagt ggaacatctt
     9061 ggtagcgtgg ctcaggattc ccctgacgga agtccaaggt cgctgtctta gtaacaaagg
     9121 ttcctgccgc tgagtttttg acctcttcta actcctctat cgtcatacaa gccacacctg
     9181 ctgcattcat caagcaattg tcaaactcaa aaccagcaat ttgtgttttc gttgatacca
     9241 tgattctaat cctccagatt tcttttattg ctaat
//
LOCUS       DAVKGC010000081         8973 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKGC010000081 DAVKGC010000000
VERSION     DAVKGC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8973
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.081"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(286..1134)
                     /gene="dprA"
                     /locus_tag="VW049_001567"
     CDS             complement(286..1134)
                     /gene="dprA"
                     /locus_tag="VW049_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9432657.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPASILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1230..1919)
                     /locus_tag="VW049_001568"
     CDS             complement(1230..1919)
                     /locus_tag="VW049_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9432658.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1931..2809)
                     /locus_tag="VW049_001569"
     CDS             complement(1931..2809)
                     /locus_tag="VW049_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9432659.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2799..3668)
                     /gene="licA"
                     /locus_tag="VW049_001570"
     CDS             complement(2799..3668)
                     /gene="licA"
                     /locus_tag="VW049_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9432660.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDATSI
                     KTKFDKIVPILQTIHASGKELRGEFAPFEEIKKYESLIEEKIPYANYEAVREEVFSLE
                     KRLADLGVYRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGYYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3685..4707)
                     /locus_tag="VW049_001571"
     CDS             complement(3685..4707)
                     /locus_tag="VW049_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9432661.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4712..5419)
                     /locus_tag="VW049_001572"
     CDS             complement(4712..5419)
                     /locus_tag="VW049_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9432662.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIENIIEAIDAYRPL
                     TSEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITGIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5755..7242
                     /gene="tacF"
                     /locus_tag="VW049_001573"
     CDS             5755..7242
                     /gene="tacF"
                     /locus_tag="VW049_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9432663.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYSYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7252..8055
                     /locus_tag="VW049_001574"
     CDS             7252..8055
                     /locus_tag="VW049_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9432664.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8057..8866
                     /locus_tag="VW049_001575"
     CDS             8057..8866
                     /locus_tag="VW049_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9432665.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYITPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2821 a         1816 c         1511 g         2825 t
ORIGIN      
        1 tcactatact ttcccttata ctcaatgaaa atcaaagcgc aaactaggaa gcgagccaca
       61 ggttgctcaa aatactgttt tgaggttgca gatggaagct gacgtagttt gaagagattt
      121 tcgaagagta ttatatgaga aaaatcctct actctactaa tccacttaga aaaaacgcat
      181 attatcaagt gttaaaaact tgctaatatg cgttttatta tggaaatgtt gggttcattt
      241 tctcttaaaa ttgatttttt cttagaattc tagcttaggt catttttaaa attcaaattc
      301 cgcaagaaca tcttgcccac tggtgaccaa ttttgctcct tcttgaatca aatgatggca
      361 accgtctgat agtccatcta aaatgctagc aggaatagca aagacatcgc gtccttcttc
      421 cattgctcgc tcacacgtaa tgagactacc tgaacgcatc ttagcctctg ctacaatcac
      481 accacgacaa agtccagcaa tgatgcgatt acgggcagga aaatgaaatt tcagaggttg
      541 ttcgccaggt ccatattcac ttaaaaccag atggtcattg ccgatgtagt cttgcaagcg
      601 tttattggct ttaggataaa acacatccag tcctgttcca atcactgcaa tggtttttcc
      661 gccattctga agagctgcca tatgagctgc tgtgtcaatg cccttggcca gaccactgac
      721 aataaccagt tcattttcca agccttgaat gactttttca actgatttag ctccctgttt
      781 gctacaagca cgactgccca cgaccgctac cttcgggaat ttcaggaggt caagatttcc
      841 cttgtaaaat aaaagtacag gcgcatcata tatttcactc aaatcccaag gataacagtc
      901 atctaaaata gagaaagatg gaaatttttg aaactctttc gacaaatgcg catcgtctat
      961 ctgaaaataa cgttccataa aaacggctgg attacggcaa cctgatatat ctgcaatatc
     1021 acccaacaaa agctcctgat caacattttc accgtattct agcactttca aaatctgttg
     1081 attggtcaaa cctgattttt ttaacttata gatttcatag tttgtgattt tcataaataa
     1141 ctccatttct ttttctactc atctatctat tcgtaaaagt tcgtaaaaaa tcaaaaaaca
     1201 tccgactatt ttgtcagatg ttggaatctt taattttcgt ttttaagaat ttcttctaat
     1261 ttacgatagt cttggacact atcgatctca taaatgctat tgccttctaa ttcttcaaca
     1321 tagacatcta gctctttgat attatcctta accatattgt cccagtagag atcaacaaat
     1381 tcgccacttg cataagcctt atcgataaag ctgacaatct tttctgcagt tggagcatcc
     1441 cagaaggata caccactaag gatgcgacct gccttgctat caacaataat gtcttgaacc
     1501 ttgtagtcat ctccatagac caagaaccat tcgttggtac aatcttcacg ataaacacta
     1561 aaataagtcg aacgagtcaa atcattgcgg aacatatttt taaagagata gttatctgca
     1621 tcaataacat agctgttggc caattcttct tttacaagat agagagagta aaagttattg
     1681 tagtcagcgt atttatcatt gaaaacgaga cgaacaccgt atttctcttt caagtaatcg
     1741 aattgttctt taagataacc aacaatgatg atgatgtcat tgattccttt ttctttgaga
     1801 aactcaattt ggtactcaat taagggtttt tgattaacct gaaccaaggc tttaggggta
     1861 ttttcagtca taggacgcaa gcgagttccc aatcccgctg ctaagataat ggctttcaca
     1921 cgaatctcct ttattcttta ataataatat aaactccagc aatgacgaca agtgacgtca
     1981 taatggtcag catatctagc ggtgcaccca agaaaacaac tgcaaacaag accgtccata
     2041 ctacatagct cacgttcaag cctgtagcct tggctggttg caagcgattg atagcgatat
     2101 aataagccaa gtaggaaatc atatcaaagg ctgcaaaaac aatcatgaga cctagcaatt
     2161 gtccattggc tactgcagta aatgactgat gagagaagag cacaatcaca agataggaca
     2221 agaacgaagt tacttgacgg attaagaggg cttcgatttc actcaattca ctttccatgg
     2281 caaaagagct aagaacactc tcacttcccc atgcaatagc acaaaccaaa gcacaaagaa
     2341 tcccaatgta gaaagaatta acctgttcaa ccttataggt ctgggcaata atccctccaa
     2401 taatcaagac aatcccaaat acagtatttt tcgaaatctt gtgcttcaaa aagaagaaag
     2461 ccaatagaac tgaaatcgca gggtaaatag ccgatacaga tgaagctaaa gaacttccga
     2521 tatacttaac tgcataaaga ttggcctgca taccgatagg gcctgctagc aaggctccga
     2581 tgataacact gacattgcga atatttaaga aaattgagag gcgaactttc ccttctttta
     2641 ccaagagaaa agctagtaag ataaagatgc tcaaaaaatc atgagctgca gccaccacaa
     2701 agggtgacaa atctgtaaaa atcgaaaaga tataagcact aaccgttaga cccaagcccc
     2761 agaaaatacc tgagagaagg ccaaaaggaa ctccgttttt acttttcatc tgaacctcca
     2821 taagaagcca aacctttaac agctctttgg taacgattca caccatagta accaaaatct
     2881 gcaccttgct cttccttata gacagtccat agactccaaa tagtatcttg taaaatttta
     2941 taaatagcaa tcttttcatg agaaaccggt gtttggtcac tctcatagtg agataagaaa
     3001 gtttcttcct cttgggaagt gaattcagac tctaaaaaga gggcagccaa atcccacatt
     3061 ggatcattca ttgatgaata ttcccagtca atcaaataaa gtcgtccttg aggtgattcg
     3121 ataaagtttt caggcaccaa atcgatatga caagattttc tgtaaacacc taagtcagcc
     3181 agtcttttct ctaaggagaa gacttcttct cgaacagctt catagttggc ataagggatt
     3241 ttctcttcaa ttaaggattc atattttttg atttcttcaa aaggagcaaa ttctcctctt
     3301 aattccttgc cagaagcatg aatagtttgt aatattggaa caattttatc aaatttggtc
     3361 ttaattgaag tcgcatcaag cgtaatagca gattcgatat actcatttac tttgatacca
     3421 gcttcaatat caaaaagata attttttaca tctaagccta aatcctttag tagttcaaga
     3481 ttgtactttt catcttgtcg attgataagc ttttctgtcc ctttaccaaa gaatttaaca
     3541 atgtattgct tatttgttgt tttggccaaa tagttttgat tggtcattcc acccagttgt
     3601 tcaacactga ggacttcctc ttcttgacta agtaaggaag aaattttttc tttaatgatt
     3661 ttctccacaa ttaacctcca gtacttatac ttcccactta aacactgttt taaaggctgt
     3721 gtttaaatcg gttgcaaaga cacgatgaat atctttaatt tctcttacag gttcttctag
     3781 ataaaggata tttttaagac gattggcaaa tttcttgact tccatcattt ggatagcatt
     3841 ttcaaaatca atgcgaccag aacgagatga cccaaccaaa atcaagccct tttctaaggc
     3901 atcgcgagta ttgagattga ctttatattc gctaactccc atcatgagaa tcgttccctg
     3961 aggacgaatg tagcgaatca agtcattaat agctggtcca gtaccatcac caccacaaca
     4021 ttcaaaagca tggtcaaagg ccaaatcttc aggaatatta tccgtaatat agcattcttt
     4081 ggcaaatgag aagagttcca acttttccca atgacgacca ataaccacaa tctctgattc
     4141 tggcaaagta tagttgataa tattggcaac cacaaaagct aaacttccat ctccaataac
     4201 ggcgatccgc tcccgcttgc tatgagcaag agtcaataga cgattcatag cgtgcatgcc
     4261 cacactgaca aactctgtaa tggctgcaac cgtatcttca atagcatcat aagccaccac
     4321 acgatcttta gggagagaaa caaactctct cataaagcca tcaaatccac tagacaagaa
     4381 atgggtccct gtcatgtagt tttcatagaa ttcttcatca ctctgcatag gagactgatt
     4441 gggaatcatg acaacttttt gaccaacctc gtaggttccg gtcgggtcag aaatgacggt
     4501 tccacatgac tcgtgaatca ttgccattgg aagcttttta ttcaaaatct tgggatcacg
     4561 ttttccctga tagtaacgct gatccgcatg acagacagcc atgtagttgg gacggataag
     4621 gatatgattc tcttggtcaa tagcctcttc ctgatatttg acattgataa acttaggctt
     4681 agttagttga taaatttgat taatcatttt actagtcttt ctcaatcata ctttttgcaa
     4741 tcttcaaatc tgttacggtt gtaatcttca gatttgagta ttcacctttg gccaaggcca
     4801 catcttttcc tttgatcaca aagattttac atgcatctgt caagatttcc ttctcttcat
     4861 cagaaagaga tccataaagg tccatgaagt ccttgcaacg gaatgtttga ggtgtttgtc
     4921 cctgatagag gtgagcacga tttggaatac ctgtaatgaa ttgcccattg gtactttcaa
     4981 cgatagtatc cactgcttct accactgtgt ccactgcgtc atgattttgg gcaagttgga
     5041 tattgtcctg aatcatgcga agtgtaataa atggacgaac agaatcgtgg gtaacaacga
     5101 tatcctctga agtaagcgga cgataagcat caatggcttc aatgatgttc tcaatacttg
     5161 tattgcggtc agcaccaccc tttgtaatga tgatacgttc cttataaaga ggaagatatt
     5221 tatctacaag atcttctgca tgagaaaccc agtctccatg aacaccaact acaatttttt
     5281 caatacttgg ctccaagaca aatttttcaa ttgtatgaat caaaataggt cgatcaccta
     5341 gctctaaaaa ttgttttggc aagttactga tccccatgcg tgtgccagtt ccaccggcaa
     5401 gaattcctgc ataaatcatc tattttctcc tttgtcttac taaaaaaact tattctctct
     5461 attataccac aatctagtcc tatcataaaa gaaatactgg tcctcccctt ctagaatagg
     5521 agaattatgc agttctatta ttcaaagaaa ctagtccatt tctagtaatg actaggaatt
     5581 ctgtagcaat tactaataaa tgactgcgaa attttagagt ccttcagaat aatatacttt
     5641 ccttaaagaa aacttaaaat ttaagatttg aaacttcaaa gtgattgctt ggcttactgc
     5701 ccttcatttc ttattagcta actttatgat ataatgaaaa acgaggtaaa ttgaatgaaa
     5761 agtataaaat taaatgctct atcttacatg ggaattcgtg tcttgaatat tatttttccc
     5821 atcctaactg gaacctatgt cgcgcgtgtc ttggaccgaa ctgactatgg ttacttcaac
     5881 tcagtcgaca ctattttgtc atttttcttg ccctttgcaa cttatggtgt ctatagctac
     5941 ggtttaaggg ctatcagtaa tgtcaaggat aacaaaaaag atcttaacag aaccttttct
     6001 agtctttttt atttgtgcat cgcttgtacg attttgacca ctgctgtcta tatcctagcc
     6061 tatcctctct tctttactga taatccaatc gtcaaaaagg tctaccttgt tatggggatt
     6121 caactcattg cccagatttt ttcaatcgaa tgggtcaatg aagctctgga aaattacagt
     6181 tttctctttt acaaaactgc cttcatccgt atcctgatgc tggtctctat tttcttattt
     6241 gttaaaaatg aacacgatat tgttgtctat acacttgtga tgagtttatc gacgctgatt
     6301 aactacctga ttagttattt ttggattaaa agagacatca aacttgttaa gattcaccta
     6361 agtgatttta aaccactctt tctccctctg acagccatgt tagtctttgc caatgccaat
     6421 atgctcttca cttttttaga tcgcctcttc ctcgttaaaa cagggattga tgtcaacgtt
     6481 agttactata ccatagctca gcgaattgtg accgttatag ctggggttgt aacaggtgca
     6541 attggagtga gtgtgcctcg tctcagttac tatctgggga aaggagacaa agaagcctat
     6601 gtttctctgg ttaatagagg tagtcgaatc tttaacttct ttatcattcc actgagtttt
     6661 ggactcatgg ttttaggacc aaatgccatc ctactttacg gtagtgaaaa atatatcgga
     6721 ggcggcatct tgacctctct cttcgctttt cgtacgatta tcctggcctt agataccatt
     6781 cttggttccc aaattctctt taccaatggc tatgaaaaac gtatcacagt ttatacagtc
     6841 tttgctgggc tactcaattt gggcttgaat agtctcctct ttttcaaccg tatcgtggct
     6901 cccgaatact acttactgac aactatgtta tcagaggctt ctctacttgt tttctatatc
     6961 attttcatcc atagaaaaca actcatccac ttgggacata tctttagcta tactgttcga
     7021 tactctctct tttcactttc ctttgtagca atttatttcc tgattaattt cgtgtatcct
     7081 gtggatatgg tcattaattt gccatttttg attaatactg gtttgattat cttgctatca
     7141 gctatctctt atattagtct acttgtcttc acaaaagata gcattttcta tgaattttta
     7201 aaccatgttc tagccttaaa aaataaattt aaaaaatcat aggagtttaa aatgaaacaa
     7261 ctaaccgttg aagatgccaa acaaattgaa ttagaaattt tggattatat tgatactctc
     7321 tgtaaaaagc acaatatcaa ctatattatt aactacggta ctctgattgg ggcggttcga
     7381 catgagggct ttatcccttg ggacgacgat attgatttat ccatgcctag agaagactac
     7441 caacgattta ttaacatttt tcaaaaggaa aaaagcaagt ataagctcct atccttagaa
     7501 actgataaga actactttaa caactttatc aagataaccg acagtacgac taaaattatt
     7561 gatactcgaa atacaaaaac ctatgagtct ggtatcttta tcgatatttt ccctatagat
     7621 cgctttgatg atcctaaggt cattgatact tgttataaac tggaaagctt caaactgctg
     7681 tctttcagta aacataaaaa tattgtctat aaggatagcc ttttaaaaga ttggatacga
     7741 acagcctttt ggttgctcct tcgaccggtt tctcctcgtt attttgcaaa taaaatcgag
     7801 aaagaaattc aaaaatatag tcgtgaaaat gggcagtata tggcttttat tccttcaaaa
     7861 tttaaggaaa aggaagtctt cccaagtggt acctttgata aaacaatcga tttacccttt
     7921 gagaatttaa gccttcctgc acctgaaaaa tttgatacta ttttgacaca attttatgga
     7981 gattatatga ccctaccacc agaagaaaaa cgcttctaca gtcatgaatt tcacgcttat
     8041 aaattggagg attaggatgc aatatttaga aaaaaaagaa attaaagaaa ttcaactagc
     8101 cctgctggac tatattgatg agacttgtaa gaaacatgat attccttatt ttctcagtta
     8161 tggaaccatg cttggagcca tccgccacaa aggtatgatt ccttgggatg atgatattga
     8221 tatttccctt tatcgtgagg attatgagcg tttactgaag attattgaag aagaaaatca
     8281 ccctcgctac aaggttcttt cctacgatac atcttcttgg tacttccata atttcgcatc
     8341 gattttggac acttctactg ttatagaaga ccatgttaag tacaagcgtc atgataccag
     8401 ccttttcatc gatgtcttcc caattgatcg atttacagac ttgagcattg tcgacaagag
     8461 ctataagtat gtagcccttc gtcaactagc ttatatcaaa aaatcacgag cagttcacgg
     8521 tgatagcaaa ctaaaagatt ttcttagatt atgtagctgg tacgctctcc ggtttgtcaa
     8581 tcctcgctac ttttacaaga aaattgatca actagtcaaa aatgctgtaa ccaacactcc
     8641 tcaatatgaa ggaggagttg ggatcggtaa ggaagggatg aaagaaatct tcccagttga
     8701 tacctttaaa gaactgattt taactgagtt tgagggccgt atgttgcctg ttcccaaaaa
     8761 atatgaccaa tttttaaccc agatgtatgg cgattatata acaccaccat caaaagaaat
     8821 gcaagagtgg tatagtcata gcattaaagc ttatcgcaaa aactgattga gggggattat
     8881 acaaactact aagatagagg ttattcaaaa acataatttt agtagaaaat gaaatacata
     8941 ttcccacaat aaaacgcatc atatcaaggt ttt
//
LOCUS       DAVKGC010000082         8906 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKGC010000082 DAVKGC010000000
VERSION     DAVKGC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.082"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            265..1071
                     /locus_tag="VW049_001576"
     CDS             265..1071
                     /locus_tag="VW049_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9432666.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(1237..1773)
                     /locus_tag="VW049_001577"
     CDS             complement(1237..1773)
                     /locus_tag="VW049_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9432667.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKDYETTTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            1906..2796
                     /locus_tag="VW049_001578"
     CDS             1906..2796
                     /locus_tag="VW049_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9432668.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            3040..3393
                     /locus_tag="VW049_001579"
     CDS             3040..3393
                     /locus_tag="VW049_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9432669.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            3394..4431
                     /locus_tag="VW049_001580"
     CDS             3394..4431
                     /locus_tag="VW049_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9432670.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(4477..5484)
                     /gene="galR"
                     /locus_tag="VW049_001581"
     CDS             complement(4477..5484)
                     /gene="galR"
                     /locus_tag="VW049_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9432671.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAI
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            5648..6826
                     /locus_tag="VW049_001582"
     CDS             5648..6826
                     /locus_tag="VW049_001582"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9432672.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGSGLSSSASLEILTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            6845..8326
                     /locus_tag="VW049_001583"
     CDS             6845..8326
                     /locus_tag="VW049_001583"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9432673.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLSRVGEGVLEVETNL
                     DKLIDLKDQLVEETVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIARNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVDQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKAFRFAGFEQVKAGIVKWPMSVLRLTSDSKEDLINLADKILQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            8396..8800
                     /locus_tag="VW049_001584"
     CDS             8396..8800
                     /locus_tag="VW049_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9432674.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
BASE COUNT         2508 a         1577 c         2159 g         2662 t
ORIGIN      
        1 cttttttgat gtgagaatga ctgaatgtca cttaaaatgg aaagaaagag aaaattttca
       61 tgattttcta aaattttact gtaaagacag ttgactattc ataaaatagg tgtataatat
      121 tttgtgaact acttaacaaa taagaggttt cagctcatgt cgactaaggt tagaaatctt
      181 gtgtataggc agttcattat ctaatagagc gttgcgtccg aaagtctatc cagacacggc
      241 tctttaaaaa caaaaggaga aatgatgcat acttatttgc aaaagaaaat tgaaaatatc
      301 aaaacaaccc taggtgaaat gtcaggtggt taccgtcgta tggttgcggc tatggctgat
      361 ttaggatttt caggaactat gaaggctatc tgggatgacc tctttgccca tcgtagtttt
      421 gcccagtgga tttatttgct ggttttagga agttttcctc tctggctgga gttggtttac
      481 gaacatcgta ttgttgactg gattgggatg atttgtagct tgacagggat tatctgtgta
      541 atctttgtat cggaaggtcg agcaagtaat tatctttttg gcttgattaa ctctgttatt
      601 taccttattt tggccctaca gaaaggcttt tatggtgagg tgctgacgac actttacttc
      661 acagtcatgc agccaattgg acttctagtt tggatttatc aggcacagtt taagaaggaa
      721 aagcaggagt ttgtcgcgcg taaactggac ggaaagggct ggacaaagta tctttccatt
      781 agtgtgcttt ggtggttggc ctttggcttc atttatcagt ctattggtgc caatcgtccc
      841 tatcgtgatt caatcacaga tgcaaccaat ggggtagggc aaatcctcat gacagctgtt
      901 taccgtgaac agtggatatt ctgggcggct accaatgttt tttcaatcta tctctggtgg
      961 ggagaaagcc tgcaaattca agggaaatat ctaatttatc tcattaacag tctagttggt
     1021 tggtatcaat ggagcaaggc agctaagcag aatactgatt tacttaacta ggaaaagatg
     1081 tttgaaagtg ctgttttgag atttcgatta aaacagatat agttgataat caaggattta
     1141 tagtatgaaa aagaggatcg gcgggtcctc ttttgttgtt gaaaagataa aaaactcagt
     1201 aacctagaaa taagacaact gagctttact ctatattcaa tttttaggaa tgagaaggtc
     1261 tagataaaat tggacaactt cctggtctgt gaaatcttga ccttttttga gccaccaggt
     1321 caatgtctcg ataaagttgg acatgaccaa gtgttggagg taagaagtag gcagattagg
     1381 gtgggcttct tttaaattat cagctagcac ggaatagacg tggtgctcaa gctctctctg
     1441 gagttgacgg aggaagtagt catttttgga aaatagcaga ctggtgatat ggtcttggtt
     1501 tttatgaaaa tggagaaaga ggtgggcgag gtagtcctca gttgaaatgg cttgctctct
     1561 ttcaaaaaga tgatggaaga ggtagcggca gagctgatcc agaagaagct ctttactctc
     1621 atagtgacag taaaaggtgg atcgtcccac atctgcgaga tcaatgatat cctgaacagt
     1681 agtggtctcg tagtccttag cattcaaaag ttgtataaaa gcttgataga tggctttttt
     1741 ggttttgctg atacggcggt caatgttagt catatggaca cttgaggcaa attgttcaga
     1801 actgaataaa gctgacgttt tgcttctatc ctttctttga gttttagtgg ataatgataa
     1861 tgaacaaggt gttcataaat ctattataac aaaggaatga gaaatatgaa ggcaaaatat
     1921 gctgtttggg tggctttttt cttaaatttg acttatgcca ttgttgagtt tattgcaggt
     1981 ggagtatttg gttctagcgc tgttcttgct gactctgtgc atgacttggg agatgcgatt
     2041 gcaattggaa tatcagcttt tctagaaaca atctccaatc gtgaagaaga caatcagtac
     2101 accttgggct ataagcggtt tagcctgcta ggagccttgg taacagctgt gattctcgta
     2161 acgggctctg ttctagtcat tttggaaaat gtcacgaaga ttttgcatcc gcaaccagtc
     2221 aatgatgagg ggattctctg gttaggaatt attgcgatta ctatcaatct gttagcgagt
     2281 ctggtggttg gtaagggaaa gacaaagaat gagtctattc tgagtttgca ttttctggaa
     2341 gatacgctag ggtgggtagc tgttatcctg atggcgattg ttcttcgatt tacggactgg
     2401 tatatcctag atcctctttt gtcccttgtc atttctttct ttattctttc aaaagccctt
     2461 ccacgttttt ggtctacact caagattttc ttggatgctg tgccagaagg tcttgatatc
     2521 aagcaagtaa agagtggcct ggagcgattg gacaatgtgg ccagccttaa tcagcttaat
     2581 ctctggacta tggatgcttt ggaaaaaaat gccattgtac atgtttgtct aaaagaaatg
     2641 gaacatatgg aaacttgtaa agagtctatt cgaattttcc taaaagattg tggttttcaa
     2701 aatattacca ttgaaattga tgctgaccta gaaactcacc aaacccataa gcgaaaggtg
     2761 tgtgacttgg aacggagtta tgagcatcaa cattagaaaa aagtgaaaaa tacttgggta
     2821 ctatcttatt tggaatagag taatttcttt attatttaaa tatttcaaaa attggtaaga
     2881 gaagagcatt gtataaactc cagatatatg attgttaatg ataaaaattt ttcgattaga
     2941 tacaaaatgc ttgacttgga gtcaactcaa agttatataa taagataagt gagttagaat
     3001 agcgtgaatt cagtgaatga aatgagagga ggttagcgtg tgaatattaa atctgccagt
     3061 gatttgttgg gaatttcagc ggatacgatt cggtattatg aacgggttgg tcttgtgcca
     3121 ccgattactc gtactgctac tgggattcgt gattttcaag atcaggatat cgaagcgctg
     3181 gaatttatta agtgttttcg ttcggcgggt gtctctgtag atagtttagt tgactatatg
     3241 tcgctctacc aaaagggaga tgaaacgaga gaggagaggc ttggtatttt agaagaggaa
     3301 aagcaaaaat tagaggagcg cttgtctcag ctacagacag ctttaaatcg tttaaatctc
     3361 aaaattaaac tttataagga aggaaaattt taaatgaaat cagcagtata tacaaaggca
     3421 ggtcaggttg gacttgctag cattgaacgt ccgcaaataa tagaagcgga tgatgtgatt
     3481 attcgtgtgg ttcgtgcgtg cgtttgtggt tcagatttat ggaggtaccg taatccagaa
     3541 acgaaagctg gacacaaaaa tagtggacac gaagcgattg ggattgttga agaagctggg
     3601 gaagccatta cgacggtgaa accaggtgat tttgtgattg tcccttttac acatggatgt
     3661 ggtgagtgtg atgcctgtct tgctggattt gacggttctt gcgacaatca tattggcaat
     3721 aatttggggg gtgattttca ggcagaatat attcgcttcc actatgcaaa ctgggcgctg
     3781 gttaaaatcc ctggtcaacc ttctgactat acagaaggga tgctcaagtc ccttttgact
     3841 cttgcagatg tcatgccgac aggctatcat gcggcgcgtg ttgcaaatgt tcaaaaaggg
     3901 gacaaggttg ttgttatcgg tgatggggct gttggtcaat gtgctgtcat cgcggctaag
     3961 atgcgtggag catcacaaat tatccttatg agccgtcatg aagaccgtca aaagatggct
     4021 atggagtcag gtgcgacagc tgttgttgca gaacgtggtc aagaaggaat taccaaggtg
     4081 cgtgaaatcc tcggtggagg agcagatgca gcacttgaat gtgttggtac ggaggctgct
     4141 atagaacagg cgctaggtgt tcttcataat ggagggcgta tgggctttgt aggagtccca
     4201 cactataata atcgtgctct tggttcgaca tttatgcaaa atatctctgt agcaggtggg
     4261 gcagcttctg ctacaacata cgataagcaa tttttactaa aagccgtcct tgatggtgat
     4321 atcaatccag gtcgcgtctt tacttcaagt tataaactgg aagatatcga ccaagcctat
     4381 aaagatatgg atgaacgtaa gacaattaag tctatgattg taatcgaata aaaaacgaat
     4441 aggagtttta gaactctatt cgttttttat gttatcctat tcttgattta gggtactttc
     4501 tcttaatgtc agtctggttc ccagcatggt caggctaggg attttccgac cgtggaggac
     4561 ttccttgtta agaatatcca tacctgctcg ccccatttct tcagtataaa ctgtgatgct
     4621 ggagagggga ggataaacct gcttggtcag gctggtgtcg ttaaaggaaa tgaggctgac
     4681 acggtctggt aggctgattc cagcttcttg gagcgcacga agggcaccga tggctaaact
     4741 atcgctggct gcgaaaaatg ctggtgggag ttggtctccc aagttctgaa tggcttcctt
     4801 catcaagtca tagccagact gggcagtaaa gtttccttga aagatcagtt catcatgata
     4861 gattcctttt gcttgactgt agtttctgaa attttccagc cgcttatcct caatgatttc
     4921 ttcttggtca gttgtttctt caaggcctgt tagaatcccg atacggtcca ttccttggct
     4981 gaggaaataa tcgacaacct gttttatggc agtgtaaaag tccgtaataa tgcaggtatg
     5041 tcccagggaa agtgtatcac tgtctagaaa gaccaggggt ttttggtatt cttcaaaggc
     5101 agaaatctga gctcggctaa attttccgat gcagagaatc ccaatcactt cctcgcttaa
     5161 ggtaaaagga tggtcattaa aatagcgcaa gatatcatag tccaactctt gggctctttt
     5221 ttctattcct aggcgaatct ggtagtagta gaggtcgtcc agctcccctt gttcgctgac
     5281 ccattgaata atggcaatct tttgcttggg tttatgggac tcgcctgtct tgaggtgctt
     5341 ggtgtagccc agctcttcag caacggttaa aatacggtgt ctggtttctt ctgtaacaga
     5401 taggctctgg tcgcggttga ggacgcggga tacggtcgcg atagagacag aggctagctg
     5461 tgcaatgtct tttaaggtag ccataaatcc tccttgatta ggttagtata tcatattttt
     5521 ctgcttttta ctgatagttt agtaaaattt tagtaaaaag gattgacctt gggaaatccc
     5581 ttggatacaa tagaagaaaa cgattacacg ttaaggtggc ttaacggaca gtcaaaggag
     5641 aaatcatatg acacaacatc ttactgctga aactcttcgc aaagactttc ttgctgtttt
     5701 tggtcaagaa gcagaccaaa ctttcttttc accaggccgt attaatttga ttggtgaaca
     5761 cacagactac aacggtgggc acgtttttcc agctgctatt tccttaggaa cttatggtgc
     5821 agctcgcaag cgtgatgacc aagtcttgcg tttctactca gctaactttg aggacaaggg
     5881 cataattgaa gtgcctctcg ctgacctcaa gtttgaaaaa gagcacaact ggaccaacta
     5941 tccaaaaggg gttcttcatt tcttgcaaga agctgggcac gtgattgaca aaggttttga
     6001 tttttatgtt tatgggaata tcccaaatgg ttctggcttg tcttcttcag catccttgga
     6061 gatcttgaca ggagtcgtgg cagagcatct ctttgattta aaactagagc gtctcgattt
     6121 ggttaaaatc ggaaaacaaa cagaaaataa ctttatcgga gtcaactctg gtatcatgga
     6181 ccagtttgct attggtatgg gggctgatca acgtgctatt taccttgata ccaacacctt
     6241 agagtatgat ttggtgccac ttgatttgaa ggacaatgtc gttgttatca tgaacaccaa
     6301 caaacgccgt gaattggcgg actctaaata caatgaacgt cgtgctgagt gtgaaaaagc
     6361 agtggaagaa ttgcaagttg ccttggatat tcagaccctg ggtgaattgg acgagtgggc
     6421 cgttgaccaa tatagctatc tgattaaaga tgaaaatcgt ttgaaacgtg ctcgccatgc
     6481 tgtgcttgaa aaccaacgta cccttaaagc tcaagcagcc cttcaagcag gtgatttgga
     6541 aacatttggt cgtttgatga atgcgtcaca cgtttctctg gagcatgatt atgaagtaac
     6601 tggtttggaa ttagataccc ttgttcacac agcttgggca caagaaggag ttctcggtgc
     6661 tcgtatgaca ggggctggtt ttggtggctg tgccattgcc ttggttcaaa aagatactgt
     6721 tgaggccttt aaggaagctg taggcaaaca ctacgaggaa gtagttggat acgctccaag
     6781 cttctatatc gctgaagttg caggtggcac tcgcgtcctt gactagtcaa aaggaggctc
     6841 tatagtgacc ttagtagata aatttgtaac acatgtcatt tctgaaagtt catttgagga
     6901 aatggatcga atctacctga ccaaccgtgt tttgtcacga gtgggagaag gtgttttgga
     6961 agttgagacc aatctggata aattgattga cctcaaggac cagctagttg aggaaaccgt
     7021 tcgattagag acgattgagg atagtcagac tgcgcgtgaa atccttggta ctgaattgat
     7081 ggacttggtg actccttgtc cgagtcaggt caatcgtgac ttctgggaag cctatgccta
     7141 ctctcctgag caagcgatag aggattttta tcaactcagt cagaaaaatg actacatcaa
     7201 actcaaggcc attgctagaa atatcgctta tcgtgttcca tctgactacg gagaacttga
     7261 aattaccatc aatctctcta agcctgaaaa agatcccaaa gagattgtgg cagccaagtt
     7321 ggtgcaagct agtaattatc ctcagtgtca gctttgtcta gagaatgagg gctaccatgg
     7381 tcgagttaac cacccagctc gtagcaatca ccgtattatc cgttttgaaa tggttggtca
     7441 ggaatggggt ttccagtatt cgccctatgc ttactttaat gagcattgta tctttttaga
     7501 tggccagcat cgtcccatgg ccattagtcg tcagagtttt gaacgtctgt tggctatcgt
     7561 agaccagttt ccaggatatt ttgctggatc taatgccgac ctgccgattg tggggggctc
     7621 tattctaact catgatcatt atcagggagg ccgtcacgta tttcctatgg aattggctcc
     7681 cttgcaaaag gccttccgat ttgctggttt tgagcaggtc aaggctggaa ttgtcaagtg
     7741 gcccatgtct gtcctacgtt tgacttcgga ttccaaagag gatttgatca atttggctga
     7801 taagattttg caggaatggc gtcagtattc agattctagt gtgcagattt tagcagagac
     7861 agacgggaca ccgcatcaca ccatcacacc aatagcgcgt aagcgtgatg gacagtttga
     7921 gttggacttg gtcttgcgag acaatcagac ttcagcagag catcctgatg gcatctatca
     7981 tccccacaag gatgtccaac atatcaagaa ggaaaatatc ggcttgattg aggtcatggg
     8041 cttggcaatc ttgccaccac gtctgaaaga agaagtagag caagtcgcta gctatcttgt
     8101 aggagaagct gttacagttg ccgattatca tcaggagtgg gcagaccaac tcaaatccca
     8161 acatccagac ctaacggata aagaaaaagc ccttgcaatc gtcaaggact ctgtgggtgc
     8221 tatctttgtg cgtgtacttg aggatgcagg agtctacaag cagacagaac aagggcagac
     8281 agcctttatg cgctttgtgg aacaggtcgg aattttacta gactaggagc tttctccttg
     8341 cacagtccta taaaaagtag tatcatagtg tcgtttgaaa tataaggagg aaacgatgaa
     8401 agactttcat tttgacgcta tatctgcctt tgaaaattac gaaattgaaa aaatgagaga
     8461 tggtcacgtt gtggtgacga ccaaagtagt gaactcatcg ctcaactact atggcaatgc
     8521 ccatggtggc tatctattca cgctatgtga ccaaatcagt ggtttggtgg ttatctcgct
     8581 gggacttgat ggggtgacac tccaatcctc tatcaactac cttaaggcag gaaaactcga
     8641 cgatgtattg accattaaag gagaatgtgt ccatcaaggt cgtacaacct gtgtgatgga
     8701 tgtggatatc accaatcaag agggcagaaa tgtctgtaag gcaaccttta ccatgtttgt
     8761 cacaggccaa aggtctgagg aaagacgggt aagtatataa agaaaggtga gtgggataaa
     8821 tttatctcac tcatttttaa tttatgagat atgttagaag gaaatataaa agagagtgat
     8881 gaaatacaaa tgtaataggt acatat
//
LOCUS       DAVKGC010000083         8871 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKGC010000083 DAVKGC010000000
VERSION     DAVKGC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.083"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..551
                     /locus_tag="VW049_001585"
     CDS             <1..551
                     /locus_tag="VW049_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9432675.1"
                     /translation="ICNNILNSSYLDSDNHYFWLDGDQNTNVSESNNLMNYLENGVVI
                     SDKIPESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKYVSYL
                     PFPTPEFFLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQEQRRA
                     VSKIQPESSMFQCIKEKLEALF"
     gene            931..1734
                     /locus_tag="VW049_001586"
     CDS             931..1734
                     /locus_tag="VW049_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9432676.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATVYHHTVDASTEEADLVSLRIVELLSRRGFRFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            2006..4788
                     /locus_tag="VW049_001587"
                     /pseudo
     CDS             2006..4788
                     /locus_tag="VW049_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            5088..5927
                     /locus_tag="VW049_001588"
     CDS             5088..5927
                     /locus_tag="VW049_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9432677.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            5945..7783
                     /locus_tag="VW049_001589"
     CDS             5945..7783
                     /locus_tag="VW049_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9432678.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFVVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            7796..>8871
                     /locus_tag="VW049_001590"
     CDS             7796..>8871
                     /locus_tag="VW049_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9432679.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDITLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIP"
BASE COUNT         2874 a         1263 c         1809 g         2925 t
ORIGIN      
        1 taatttgtaa taatatttta aactcatcgt atttagattc cgataaccat tatttttggc
       61 ttgatggaga tcaaaacact aatgttagtg aatcaaataa tttaatgaac tatcttgaaa
      121 atggtgttgt tatatcagat aaaattcctg aatcagataa taaaaatctt gatgatatta
      181 taaaattgat aacgggatgt ccaattaaat ttaatgtttc aggtaataaa gggcaaaaaa
      241 ataatattga attaattgcg aaacaaagaa gctttataga ttattgggct aaatacgttt
      301 cttatcttcc tttcccaacc ccagagtttt ttttagctaa tttgtgtaac agcgtagata
      361 gggaaggtta tgatttttct aaagatggca acggaaaaga atattttaga aaaaaaactc
      421 aagtagcgtt aggaatagag aatataactt ctgaagatat tttccaggag caaagaagag
      481 ctgtttcaaa aatacagcca gaatcatcaa tgtttcagtg tattaaagaa aaattagagg
      541 cattgttcta attccttaga ttaggtacta cataacaaat tttttaaatt caaacccgca
      601 ccaaaaacaa cattgcaact ttcttctcag gtaatgtgtt gggatttgaa cagaccaacg
      661 agttcatagt ggtgtatgcc aatgactttg ataagtctac gcgtcagact tattagttca
      721 tttatccaga tatatctcga taatcatatt atttagtcaa agaacatccc atatgagggc
      781 atgaaatgct aagtttagct tgctcactat taagatgaag tatatatatt tcatagaacc
      841 tattgtgaag gatttttata attttcgcca attccatacc tatttcacaa tttaccgatg
      901 acacagaaaa acaattgaaa ggatatttct atgcaatcaa cttacaacat tgacaatcca
      961 aacttgtctt atgaagcgaa gcgtgattta tggcggatag gttttggtct gcagaaagtt
     1021 gacaatctag tgccatcagc gtatatggaa tctttggctg agaaacagtc ccggggtgaa
     1081 ctgacttatg agcaggttta tgaggatgca acggtttatc atcataccgt tgatgcgagt
     1141 acggaggagg cagacttggt ttctttgcgt atcgtagaac tcttgtctcg aagaggtttt
     1201 agattcagtc ctgcgacttt acttgctatt cataaggagt tgtttcaaga tatatttgaa
     1261 ccctctattc cggtggggca atttcgtcag accaatatca caaagaatga acctgttttg
     1321 aatggtgaaa gtgttgtgta ctctgattac tccatgattc aaatgacctt agattatgat
     1381 tttaatcagg aaaaacaagt tgcatatgcg acactaactc aggcggatgt ggttaaacaa
     1441 atccagcatt ttatttcagg aatctggcag attcatccat ttcgcgaagg aaacactcgg
     1501 acagtaacgg tctttttgat tcagtatctt cgtgagtttg gttttgatat tgataataca
     1561 ccatttcagc aacattccaa atattttcgt gatgccttgg tattagataa tgcaaagatt
     1621 ttacagcgac gttctgagtt tttaacagct ttttttgaaa atctcttgct cggtggtcaa
     1681 aatgatttgt cttcagaaaa aatgtatcta gatttagacc tcgatttttc ataatccaaa
     1741 tactgagtaa acattgaatt ttaggcaaaa atgaagtaaa tattctcaca agaaaacgta
     1801 tatcatcaaa gttttaggtc gcttgataat atgcgttttt tgaattttat agactgctcg
     1861 tttaaactct atttacctcg tacattccgg agcgagatgg actattggtc gcatacaaaa
     1921 tgagtgttat tcggatttta ttttcgtgta caatttcagc cattcttgtt ataatcagcc
     1981 tataggaatc aaggaggtga ttgttatggc tgtttttgtg tctttggatg gaattgtggt
     2041 agaagtactt gatgtctttt cttcttttaa tggggatagt gagtttttct tgtgtaaacg
     2101 tcttaaagac aagagtcagt ttgtgatgga acgctctcgg ttcgaggaga tgttccaact
     2161 tcaaagtagt cacttgacga cgcaagaaaa attacaattg tttacctccg tgtttgctgg
     2221 acgttatgat gtttatgcta agaattttat caatgaacaa gggaaaattc agtattttcc
     2281 ttcctatgat tatggttgga agcagttgcc acctgaaaaa cggagtttcc agacattgac
     2341 gaactccgtt ttgaaatctc attttcgtgg ggaggcagct atcggtatct ttcctatgca
     2401 cttagatgat agctgttatt ttttggtact ggatttggat gaaggagatt ggaaagaagc
     2461 tggtttaacc attcgaagaa tagccaggga acgccagatg gaagcccatt tagagatttc
     2521 tcgttcgggt tacggactcc atatttggtt cttctttgag gaagcgattc tgagtcgaaa
     2581 ggctcgcctg tttggaaaga aactattaga attggctatg caggaaagta tgcaactgtc
     2641 ctttgattct tttgatcgca tgtttccaaa tcaggatgtt cttcctaaag gtggatttga
     2701 taatttaatt tcccttcctt ttcaaggaga agcttatcat caagggcgaa cggtctttgt
     2761 ggatgaacag tttcagcctt atgaagatca atggaggtat ctacaagaaa ttcagaggat
     2821 ttcaactgct aaagtggcac tgttaatcca agaggagtta ggcaagcaag aattggataa
     2881 ggagttgaag gtcgttttat ccaatatgat ccaacttgaa aaatcgtctg tgacatccaa
     2941 ggcacttttt ttcttgaaaa atatggcttc cttttctaat cccgaatttt atagtagatt
     3001 gaaactagaa tagtacacct ctgcttctaa aacattgtta gaaatcgatt tgactttcct
     3061 gatcgatttg tcctgttatt atttcatttt actatattta aagcaggcta tgcgacagcc
     3121 aatctatcaa attcctgagc gaatgtattt atttggagaa tccgattatt atttatggct
     3181 gccaagaggc ttgttagatc cattgcaaga taaatttaag caggtagttg tggaggatag
     3241 gagaaaagta caaaggtcta ttagagtagc atttaaggga gagtttactt tggagcaaga
     3301 attagccctg tcagatatga attctaaaga aaatggttta cttcatgcgg gacaggtttt
     3361 gggaagaccg ttttaggtgc tgctcttatc tctgaacgga aaacaaaaac aattattcta
     3421 gtccataata agcaactctt agaccaatgg ctagatcgct taaactgctt tttgactttc
     3481 gaagaggagg aggctatccg ttatacggca tcaggtcgtg aaaaggtaat cggctatgtt
     3541 ggacagtacg gtgggactaa gaaatggctg agtaaactgg ttgatgtcgt tatgattcaa
     3601 tctctattta agttggaaaa tagtcaaagt cttttggatg agtatgagat gatgattgtg
     3661 gatgagtgtc atcatgtctc tgccttgatg tttgaaaaag ttgttgctca gtttagaggg
     3721 aagtatcttt acggtttgac ggctacgcct gagcgtaaga atggtcatga gcctattgtt
     3781 tttcagagaa ttggtgagat actccatact gctgataaga gggaaacgga ttttaaacgg
     3841 caattgcaat taagattcac ttcttttgct catttggaaa ttgaaaagac caaagcaagt
     3901 aattttatac agctcagtga ttggattgct acagactcag cgaggaatca gttgattctc
     3961 aaggatattc tagcccaagt ggcagaaggt cggaatatct tggttctagt taatcgaatt
     4021 caacagatag atgtttttga aaagttattg aaagagaaag aggttgatgg ctgttacatt
     4081 attagcggaa aaaccaaagt ccgagagaga acaagtttac tggagatatt agaacagcta
     4141 gataaagggt ttgtattgtt gtcaactgga aaatacattg gcgaaggttt tgacttacct
     4201 cagttggaca cgctgatctt ggcagcgccc ttttcttgga aaaataattt gattcagtac
     4261 gccggtcgga ttcatagaaa ctacaaagat aagtctttgg tacgtatttt cgattatgtg
     4321 gatattcatg ttccttattt agaaaagatg tttcagaaac gacaagtagc ttatcgaaag
     4381 atggattatc gtgccattga gggtgaggag aaacaattcg tttatgttga tagtagatat
     4441 gagaatgtgt tgaggaagga cttagcaggg gaaagacagg aatgtctgct tgttttacct
     4501 tatgtgcacc agacaaaact gatgaatttt ctaaaagaat ttaggattag tcaaattgag
     4561 atatgtatac cagagacggt tgcaaataaa gcatggctag accagttgaa gagcaagaaa
     4621 attaaagtgt cttttactca atcaaaaata gtaacgccta ttcttttggt gaataagact
     4681 attgtttggt atggtgcaat gccattatta gggaaggtag atgagatgac catattacgt
     4741 ttggaatcag ctagtatagt ttctgaacta gtggcaggtt tacgatagag aaaattttta
     4801 aaaatttcta tgtatgattt tcattttttt agtgagactg ttgccattat cacattcgaa
     4861 tcacacaaaa taaaaaaatt tctacaagta cttgacaaat agattgaaat atcttaaaat
     4921 gaaaatggtt acagagttat taattattta agcttcatgt caccattaaa aattgaaata
     4981 aaaggatgtt atcactaata caagtgagca ggaacctatt taatcacatc ggaagaagtt
     5041 tcttgatgtt tttaagtagg ttccttttat tttaaaaggg aaattttatg atcataaaac
     5101 gaatactaaa ccacaatgcc gtaattgcgc aaagtaaaaa agatatcgat attcttcttt
     5161 ttggaagggg aatagctttt ggaagaaaaa ctggagataa agtaaatcca attgatattg
     5221 agaaaagttt ttttctcaaa aatagagata atatgacccg ttttacagag atgtttatta
     5281 acgttccttt ggagttggtg tacatcaccg aaaaaataat taacctaggt aaaataacat
     5341 tgggtaataa ttttgatgaa attatctata ttaatttaac ggatcatatt tcttcgagca
     5401 tagaacgtta taaagaaggg attattattt cgaatcccct acgctgggaa atatcgaaat
     5461 attataaaga agaatttgaa cttgggaaaa gggctttaca aataataaaa aaagagttag
     5521 gtattgaact tccaattgat gaagctgcat tcatagcgct acattttgtt aatgctaatt
     5581 tagaaaataa ttttcaagag tcgtataaaa tcactgaaat aattatggga attgagaaaa
     5641 tcattcaaga tttctattgt actgagttta accaagattc tattgattat tatagattca
     5701 taactcatat aaaattattt gcccatcgtt tggttgaaaa tacaacttat tgtgacgatg
     5761 atgatgaaga cttgttagca ttaatgaaaa ataaatatcc tagagaatat gaatgtggtg
     5821 aacaggtggc aatgtttata caaacggagt acaattattt gctgacttct agtgaattag
     5881 tttatttgat ggctcatatt cgtcgcttaa caaaaaattt agattaatag aataggagaa
     5941 taccatgagt tataaagata cggtacaaaa aatcctcgat gtaattggag gcgaaaaaaa
     6001 tgtcaataga gttacccatt gtgtaacacg tttaagatta gaattaaaag atgaaaattt
     6061 agtcaatgat gatgatgtga agaagatacc aggtgtaata ggtattatga aaaagaatgg
     6121 acaatatcaa attatacttg gtaatgatgt agctaattat tataaagaat tcgttaaact
     6181 tggcaatttt gaatccgatt cagttgttca agggcacaaa gggaatattt tagaaagaat
     6241 cattgagtat atcgctggtt ccatgactcc aatcattcca gcaatgttag ggggaggtat
     6301 gctgaaagtc ttggtaatta ttttaccaat gcttggtata ttgcaatcag attctcagac
     6361 tattgctttt ttgacatttt ttggggatgc tccatattat ttcttaccgc tgttattagc
     6421 ttattctgca tcacaaaaat taaaagtaac atctacatta gctatgtctg tagcaggtgt
     6481 acttctccat ccaaattttg ttcaaatggt gcaatcaggg aatcctctta gtttatttgt
     6541 tgtacctgtg acaccagcta gttatggttc gtcagtcgtt ccaattctta ttatggtttg
     6601 gttgatgaaa tatattgaaa aaatgattgc taaattaaca ccagctgtta ctaagagttt
     6661 tttgcaacct acgctagtat tattagtatc aagctgtatt gccttagttg tagtcggacc
     6721 tattggagta attgttggtg aaggattatc aaatctagtt gggcaaatgt atggtgtagc
     6781 tggatggctt acattagcta ttcttggcgc tattatgcca tttattgtta tgactggaat
     6841 gcattgggct tttgcaccta tttttttggc ggcatctatt gctactccag acgtattaat
     6901 tcttccagca atgttagggt caaacttagc tcaaggggct gcttcgatgg ctgttgcatt
     6961 aaagagtaaa aataataata caaaacaaat tgcttttgca gcaggtttct cagccttact
     7021 tgcagggatt accgaacctg cattatatgg tgtgacttta aaatataaaa aaccgcttta
     7081 tgcagctatg attggtggtg gattagcggg attatttgca ggtcttacta gtgttaaagc
     7141 gtatctattt gctgtcccat ctttgatagc gttgcctcaa tttatttatt ctgatgtgcc
     7201 atcaaatatt gtaaatgctt taattgtggc ggtcatttcg gttgttatta cctttgtatt
     7261 agcttatata tttggaatcg atgaagaaga gagttctagc aatttagaag ttaaagctgg
     7321 agtttcaaat aaaaaaatga tattttctcc tatatcagga gaaatcattc cgttaagcga
     7381 tgtccaggat aaaacatttt cagataaact aattggagac ggagtagcga ttatcccaag
     7441 tgaaggtaag gtttatgcac catttgatgg gaaaattaca aatatttttc cgactaagca
     7501 cgcaattgga ttgaagagtg atgagggtgt tgagttacta attcatattg gattagatac
     7561 tgttgagcta aaaggtcaag gttttattag tcatgtagaa gaaggagaca gagttttcaa
     7621 aaatcagttg atttttgaaa tggacttgaa tttaatcaag actaaaggct acgaaacagt
     7681 tacaccggta attgtaacga ataccaatga ttttctagat gtattagtat tacctaataa
     7741 tcagacaatc gagcattcta aggaattact ggtaatatta taatggagga aagttatgac
     7801 catttttcca gatgattttc tttggggtgg agctgttgca gctaatcaag tagagggagc
     7861 atataatgaa gatggtaagg gcttatcagt tcaggatgtg ttacccaaag gtggattagg
     7921 agaagcaaca gaaaatccta cagaagataa cttaaaattg ataggtattg atttttatca
     7981 taaatataag gaagatataa ccttgttttc tgaaatgggc tttaatgttt ttcgtacttc
     8041 tattgcatgg agtagaattt ttccaaaagg agatgaggaa gaacctaacg aagctgggtt
     8101 gaaatattat gatgaattgt ttgatgaact acatgctcac gggatagaac cacttgtaac
     8161 tctttcacac tatgagactc cattatattt agcaagaaaa tatcatggat gggttgatag
     8221 gagaatgatt catttttatg aaaaatttgc tcgaacagtt ttagaaaggt ataaagataa
     8281 agttaaatat tggcttacat ttaacgaagt aaactctgtt ttggaattac catttactag
     8341 tggaggaata gatataccta aggagaatct ttcgaaacaa gaattatatc aagctataca
     8401 tcatgaatta gtcgcctcga gtttggttac aaaaattgct cgtgagatta attcagagtt
     8461 taaggtgggg tgtatggtat tagctatgcc agcttatcca atgactccaa atccaaaaga
     8521 tgtatgggcg actcatgagt atgagaatct aaattatcta ttttcagatg tgcatgttag
     8581 aggttattat ccgaattatg caaaaagata ttttaaggaa catgacatta acatagagtt
     8641 tgcagctgaa gatgcagagt tattaaaaaa ttatactgta gattttttat cctttagcta
     8701 ttacatgagt gtgactcaat ctgctcttcc aacacagtat aattcaggag aagggaatat
     8761 tattggtggt ttagtaaatc cttatttaga gtcttctgag tggggatggc aaattgatcc
     8821 aattggacta cgtataattt taaatagata ttatgaccgt tatcaaatcc c
//
LOCUS       DAVKGC010000084         8708 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKGC010000084 DAVKGC010000000
VERSION     DAVKGC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.084"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..139
                     /locus_tag="VW049_001591"
     CDS             <1..139
                     /locus_tag="VW049_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9432680.1"
                     /translation="ARNIKTSSDIELESDVDGNKSDNLPVDIKTLGQHIEVYSVPKKQ
                     S"
     gene            339..3614
                     /gene="rexB"
                     /locus_tag="VW049_001592"
     CDS             339..3614
                     /gene="rexB"
                     /locus_tag="VW049_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9432681.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKVSRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQTEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKRLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKNLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLKKLDLADSKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3611..7261
                     /gene="addA"
                     /locus_tag="VW049_001593"
     CDS             3611..7261
                     /gene="addA"
                     /locus_tag="VW049_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9432682.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVELKQHLSRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIDSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADTYNEERKTQ
                     FAKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEKNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7431..8609
                     /locus_tag="VW049_001594"
     CDS             7431..8609
                     /locus_tag="VW049_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432683.1"
                     /translation="MTLPVRKSLHNAVLQASEADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNVIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2692 a         1831 c         1856 g         2329 t
ORIGIN      
        1 ggcacgtaat attaaaactt cttctgatat cgaattagag tccgatgttg atggcaataa
       61 atcagacaat ctacccgtcg acattaagac actgggacaa cacattgaag tgtattccgt
      121 acctaagaaa caatcataaa actcaaagcc ttgttaccga ggctttttta ttatatgatt
      181 ttcagtggcc agacttattc tatctaatac aagtaaagct aagtatttaa gatttagcag
      241 atgatgaaga aaagaaagga tggcctaaat attttaacag atttacgagg ctaatcatgg
      301 tataatatag ggtgatgaaa aattccaacg aggctgagat gaaattactt tatactgata
      361 ttcgaacttc tttgacagaa attctaacaa gagaggcaga agagctagtt gcagctggca
      421 agcgggtctt ctacattgcc cccaactctc tttcttttga aaaggaacgc gccgtgctgg
      481 aatgcttgtc ccagcaggct tctttttcga ttaccgtcac gcgctttgct caaatggctc
      541 gttatctggt cttgaatgat ttaccagtta aaactactct tgatgatatc ggtcttgggt
      601 tggcctttta caaatgcctt gccgaactcg atcccaagga cttgcgtgtt tatggcgcta
      661 ttaagcagga tcctcaattg atccagcagt taattgagct ttaccatgag atgaccaaat
      721 ctcagatgag ttttttggac ttggagaatt taacagatga ggataagagg acggatttac
      781 tcttgatttt tgagaaagta acagcctatc ttaatcaagg tcagttagcc cagggaagtc
      841 agttgtccca tttgattgag gctattgaga atgacaaggt aagtagtgat tttaatcaaa
      901 tcaccttggt cattgacggc tttactcgtt tttctgctga ggaagagcgg gttgtggact
      961 tacttcacgg caaaggtgtt gagattgtta tcggggctta tgctagtaag aaagcctata
     1021 ccagtccttt tagcgagggc aatctctacc aagccagcgt aaaatttctc catcatctgg
     1081 cttctaaata ccaaacgcct gctcaggact gttctcaaac tcatgagaag atggatagtt
     1141 ttgacaaggt ctctcgtttg ttggagtctt cttatgactt ttcagaactc gctttggatg
     1201 tcgatgagaa agaccgtgaa aatttacaaa tctggtcttg tttgacgcaa aaggaggagt
     1261 tggagctagt agcccgtagt attcgtcaga aattacatga gaactcagac ctgagctaca
     1321 agcattttcg tattctcttg ggggatgtag cttcttacca gttatctctc aaaactattt
     1381 ttgaccagta tcagattcct ttttatcttg gtagaagcga agccatggct catcatccct
     1441 tgactcagtt tgtcgagtct attttagctt taaaacgtta ccgttttcgt caggaggatt
     1501 tgattaatct tcttagaact gatttgtata ctgacctcag tcagtctgat attgatgctt
     1561 ttgagcaata tatccgctat cttggtatca atggcttgcc agcctttcag caaaccttca
     1621 ccaaatccca ccatggaaaa tttaatcttg agcgtttgaa tgtcctccgc ctgagaattt
     1681 tagcacctct tgaaaccctc tttgccagcc gaaaacaaaa ggctgaaaac ctcctacaaa
     1741 aatggagtgt ctttctaaaa gaaggagctg tgactaagca gttacaagat ttgacaacca
     1801 ctttggaagc tgtagaacag gaaagacaaa ccgaagtttg gaaggctttc tgccatgttt
     1861 tagaacaatt tgcgactgtt tttgctggtt cacaggttag tctggaagac ttcctagcct
     1921 tgctccattc tggaatgagt ttgtcccaat accgtaccat tccagcaaca gtggacactg
     1981 ttctggtgca gagttacgat ttgattgcac cattgactgc tgactttgtc tatgctattg
     2041 gactaactca ggacaattta ccaaaaattt ctcaaaacac cagtcttctg acagatgaag
     2101 aaaggcaaaa cctaaaccaa gcgaccgaag aaggcgttca attactgatt gccagcagtg
     2161 aaaatctcaa gaaaaatcgc tacactatgc tttccttggt caattctgct cgtaagcagt
     2221 tgttcttgtc ggctccaagc ctttttaacg aaagtgaaag taaggaatct gcctatcttc
     2281 aagagttgat ccattttgga tttaggcgga gagagaagag gatgaatcac aaaagactgt
     2341 ctaaggagga tatggggtcc tatcacagtc ttttgtctag tctggttgcc tatcaccagc
     2401 agggtgagat gagcgatact gagcaagatt tgacttttgt caaggttctg tcgcgtgtca
     2461 taggtaaaaa actagatcag caaggtctgg aaaatccagc tatcccaacc agtccaagca
     2521 gcaagacctt agccaaggac accttgcaag ctctctatcc tgccaaacag gagttttacc
     2581 tgtctacgtc gggtttgaca gagttttatc gcaatgaata cagttatttc ctacgctacg
     2641 ttttaggctt gcaggaggaa ttacgtttgc atcctgatgc ccgtagtcac gggaatttct
     2701 tgcatcgtat ctttgaacgc gccttacagt tgcctaatga agattccttt gaccaacgtc
     2761 tagaacaagc tattcaagaa accagtcaag aacgcgaatt tgaagctatt tatcaagaaa
     2821 gtttggaagc ccagtttacc aaggaagttt tgcttgatgt tgcacggacg actggacata
     2881 ttctccgaca caatccagcc atcgaaacca tcaaagaaga agcaaatttt ggtggaaaag
     2941 accaagcctt tattcaatta gacaatggac gcagtgtctt tgtacgaggc aaggtggacc
     3001 ggattgaccg tttgaaagct aatggagcga taggagtagt agactacaaa tccagtctga
     3061 ctcagttcca gtttcctcat ttctttaatg ggctcaattc tcagttacca acctatcttg
     3121 ctgccctaaa aagagaaggg gagcagaact ttttcggcgc catgtacttg gaaatggctg
     3181 aacctgtcca atctctgatg gcggtaaaaa atcttgcagg agcagtggta gaagccagca
     3241 aatctatgaa ataccaaggg ctcttcttgg aaaaagaaag cagttattta ggcgaatttt
     3301 ataacaaaaa caaggctaat caactgacag atgaggaatt tcagctccta ctggactaca
     3361 atgcctatct ttacaagaaa gctgctgaga agattttagc aggccggttc gccatcaatc
     3421 cttatactga aaatggcaga agcattgccc catacgtcca gcaacatcag gctattacag
     3481 gctttgaagc caattaccat ctgggccaag cccgtttcct aaaaaagttg gacctagctg
     3541 atagcaagcg tctggtcgga gaaaaactca agcaagcttg gcttgaaaaa ataagagagg
     3601 agttgaatcg atgaagctta ttcccttttt aagtgaggag gagattcaaa aactgcaaga
     3661 agcagaagca aattcgagca aggaacagaa gaaaactgcc gagcaaatcg aagctatcta
     3721 cacttctggc cagaatatcc tggtctcagc atcggctggt tctggaaaga cctttgtcat
     3781 ggcagagcgc attctggacc aattggcgcg tggtgtcgaa atttctcaac tctttatctc
     3841 aacctttacc gtcaaggctg caactgaact taaagaacgt ttagagaaaa aaatcagcaa
     3901 gaaaatccaa gaaacagatg atgtcgagct caaacaacac ttgagtcgcc agttggcaga
     3961 cctacccaac gctgccatcg gaaccatgga ctctttcaca caaaaattcc ttggcaaaca
     4021 tggttatctg cttgatattg cacctaattt ccgtatttta caaaaccaaa gcgagcaact
     4081 tcttctcgaa aacgaagtct ttcatgaggt ctttgaagcg cattaccaag gtaaacagaa
     4141 agagaccttt agtcatttgc tgaaaaactt tgctgggcgt ggcaaggacg aacggggtct
     4201 gcgccagcag gtctataaaa tctatgactt cctccaatcc accagtaatc ctcaaaagtg
     4261 gctgagtgaa tctttcctca aaggatttga gaaagctgat tttaccagtg aaaaagaaaa
     4321 actgactgag caaatcaaac aagccctttg ggatttggaa agctttttcc gttaccatct
     4381 ggataacgat gccaaggagt ttgcaaaggc tgcctattta gaaaatgttc agttaattct
     4441 ggatgaaatt gactccctaa atcaggagtc cgatagtcag gcttatcagg cagtgcttgc
     4501 gcgtgttgtc gccatctcta aggagaaaaa cggtcgagct ctgactaatg ccagccgtaa
     4561 ggctgatttg aagcccctgg ctgataccta caacgaagag agaaagaccc agtttgctaa
     4621 actaggacaa ttatcagacc agatagcgat tctcgactat caagaacgtt atcatggaga
     4681 cacttggaaa ctagctaaaa ccttccaatc tttcatgagc gattttgtag aggcttatcg
     4741 tcagagaaaa cgacaggaaa atgcctttga attcgctgat atcagccatt acaccattga
     4801 gattttagag aatttcccac aagttcgtga gtcttatcag gagcgcttcc atgaagtcat
     4861 ggtcgatgag tatcaggata ccaaccatat tcaagaacgg atgctggaat tgttgtctaa
     4921 tggccacaat cgctttatgg tgggagatat caagcaatcc atctatcgtt tccgtcaggc
     4981 agacccgcag attttcaatg agaaattcca acgctatgcg caaaatcctc aagaaggcaa
     5041 gctcattctc ctcaaggaaa atttccgtag tagttcagaa gtgctgtcag caaccaatga
     5101 tgtctttgaa cgtctcatgg accaagaggt cggcgaaatc aactatgata acatgcacca
     5161 gcttgttttt gccaatacca aactgactcc caatccagac aacaaggccg aatttctcct
     5221 ctacgataag gacgatacag gtgaggaaga agagagtcaa acagaaacga aactaacagg
     5281 cgaaatgcgc ttagttatca aggagattct gaaacttcat caagaaaaag gtgttgcctt
     5341 taaggaaatt gcccttctga cctccagccg cagtcgtaat gaccagattc tcctcgccct
     5401 gtctgagtac ggaattcctg tcaaaactga cggagagaaa aacaattatc tccaatccct
     5461 agaagtgcaa gtcatgctag acactcttcg tgtcattcac aatcccctgc aagactacgc
     5521 cttggttgcc cttatgaagt ctccaatgtt tggttttgat gaggatgagc tagcacgttt
     5581 gtcccttcag aaagcagagg ataaagtcca cgaaaatctc tatgagaaac tggtcaatgc
     5641 acaaaaaatg gcaagtagtc aaaaaggttt gattcacaca gctctagctg aaaaactaaa
     5701 gcaattcatg gatatcctag cttcttggcg cttgtatgcc aaaacccact ctctctatga
     5761 cttgatttgg aagatttaca acgaccgttt ttattatgac tatgttgggg ctttgccgaa
     5821 tggtcctgct aggcaggcca atctctatgc cctagcactg cgtgctgatc aatttgaaaa
     5881 gagcaatttc aaaggtttat cgcgttttat tcgtatgatt gaccaagtct tagaagccca
     5941 gcacgatttg gcaagcgtgg ccgtcgcacc gccaaaagat gcagtagagc tcatgaccat
     6001 ccacaagagt aaggggctgg agtttcctta cgtctttatc ctcaatatgg atcaagattt
     6061 caacaagcaa gactctatgt cagaagtcat tctcagtcgt cagaatggtc ttggtgtcaa
     6121 atatattgcc aagatggaga caggggcagt agaagaccac tatcctaaaa ccatcaaact
     6181 ctccattcct agcctgacct ataggcagaa tgaagaggaa ttacagctag caagctattc
     6241 tgagcagatg cgtttgctgt atgttgctat gacgcgggct gagaaaaagc tctatcttgt
     6301 cggcaagggt tctcgtgaaa agctggaagc caagcaatac ccagcagcca aaaatgggaa
     6361 actaaatagc aatactagac tgcaagcacg gaatttccaa gattggcttt gggctatcag
     6421 taaagtgttt actaaggaca agctcaactt tagttatcgt tttattggcg aagatcagtt
     6481 gaccagagaa gctatcggag agttggaaac caagagtcct ctccaagata gctcccaagc
     6541 agacaatcgt cagtcagata ccatcaaaga agctctggaa atgctgaagg aggtggaagt
     6601 ttataatact cttcaccgcg cagctattga acttcctagt gttcaaaccc caagtcaaat
     6661 caagaaattc tacgaaccag ttatggatat ggaaggtgtc gagattgctg gtcaaggtca
     6721 gtcagtaggc aagaaaatca gcttcgattt gccagatttt tcaacaaaag aaaaggtaac
     6781 tggagctgag attggtagtg ctactcacga actcatgcag agaattgacc tcagccagca
     6841 actaaccctt gctagcctaa cagaaacact caaacaagtt caaactagcc aagctgtcag
     6901 agacaagatc aatcttgata aaattcttgc tttctttgac acagtactcg gtcaggaaat
     6961 tcttgctaat accgaccatc tctatcgcga gcaacctttc tccatgctca aacgagacca
     7021 aaagagtcag gaagactttg ttgtccgtgg tatccttgat ggctatctgc tttacgaaaa
     7081 caaaattgtt ctgttcgact acaagacaga ccgctatgat gaaccaagtc aactcgtaga
     7141 ccgctatcgt ggtcagttag ctctatacga agaggcttta tcacgagcct attcgattga
     7201 aaatattgaa aaatacttga ttttactcgg taaagacgag gttcaagttg taaaagtata
     7261 acttatactc ttcgaaaatc tcttcaaacc acgtcagctt cgccttgctg tatatatggt
     7321 tactgacttc gtcagttcta tccacaacct caaaacagtg ttttgagctg actgcggcta
     7381 gctttctagt ttgctctttg atttttattg agtattagaa aggagacctc atgacacttc
     7441 cagttagaaa atccctacac aatgcggttt tacaggcttc agaagcggat acttgggaac
     7501 aagctaccaa ggaatggaat gaagtttcct tgatttttaa cggtatcggc cgtagcaatt
     7561 gtgtctgtgg aaatgtcatc aaatacgctt atgaactctt taacggtgtc acaggccaac
     7621 gcctctttcc aattggtagc gactgtgttc gtcattttca tcgattgtcc cttgaccagc
     7681 aattggaaga ggaagaaaaa ctgctcagaa aggttgaaaa tctaaccaga aaagctcaga
     7741 aaaaggaaaa aatcaaggtc aataaaagtg actttgacga gcgacttcta aaatggctct
     7801 gggaaaaagg tgttttcaaa cccaatcgtg gcaatcaatt tgcgcctgag agagactacc
     7861 agcttttcct agaagtcttt cagggaggaa gttggaccaa ggcggaacca aagaaaaagg
     7921 ctcggatgga agaagtcctt gaaaagtgta tcaaaccctt tttacttggg aaatcggatg
     7981 accaactcta ccttgtcaag ctaggcaagg agaaaattga ctacgaacaa gaactccgta
     8041 tccaggcaga gaaagaacgc aagaagaggg ataaaatcgc caagcaatat gcagacaatc
     8101 tggttctagc catgggaccc gcagagcgtg cttatcaaga ttactttggc tttacagaga
     8161 ccctgactca agaagaacgc aagtgggaaa aaattctttt tggtaaaaat agagcagaac
     8221 gggctatcaa ggccaaacaa taccaaaaag agctggaaaa agaccaacga attgccagtc
     8281 aggatccaat tgaaagaaaa cagaagcaga cttggctcct caattcctat tttcgtgagc
     8341 ttcctgaaga aaaagctcga ttttctagac tcttattaga atatagaaaa agtggtgaag
     8401 tacccttttc aactgaatac ctgtcagatc atctcatcga ctttttctat aaaatgaaag
     8461 cctttgaatt tgaaatcgca ccagaacaag tccgagattt tctaaaaaaa agcctgcagg
     8521 aagaccatcg ctcatcagca caaggaagct ggatagaagg aattctcctc aactgcctca
     8581 aaccattttt agaacgatta gtgatatgag taacacctac cgtgtaaatt tcatggtagg
     8641 tgttattgta cctccaaata atataaaaca aagtatgaaa gcgcatctca attttaagac
     8701 ttttttgt
//
LOCUS       DAVKGC010000085         8638 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKGC010000085 DAVKGC010000000
VERSION     DAVKGC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.085"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(120..1316)
                     /gene="tuf"
                     /locus_tag="VW049_001595"
     CDS             complement(120..1316)
                     /gene="tuf"
                     /locus_tag="VW049_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9432684.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2227..3096)
                     /locus_tag="VW049_001596"
     CDS             complement(2227..3096)
                     /locus_tag="VW049_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9432685.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3320..3841)
                     /locus_tag="VW049_001597"
                     /pseudo
     CDS             complement(3320..3841)
                     /locus_tag="VW049_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(3799..4905)
                     /locus_tag="VW049_001598"
     CDS             complement(3799..4905)
                     /locus_tag="VW049_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9432686.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEVDTNSASLTGNIVSEGRTVTLVYRESEAPTTVTVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5016..5270)
                     /locus_tag="VW049_001599"
     CDS             complement(5016..5270)
                     /locus_tag="VW049_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432687.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(5610..6056)
                     /locus_tag="VW049_001600"
     CDS             complement(5610..6056)
                     /locus_tag="VW049_001600"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9432688.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLECFFKTR"
     gene            complement(6129..6454)
                     /locus_tag="VW049_001601"
                     /pseudo
     CDS             complement(6129..6454)
                     /locus_tag="VW049_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6688..8406
                     /locus_tag="VW049_001602"
     CDS             6688..8406
                     /locus_tag="VW049_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9432689.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            complement(8456..>8638)
                     /locus_tag="VW049_001603"
     CDS             complement(8456..>8638)
                     /locus_tag="VW049_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9432690.1"
                     /translation="KIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFI
                     ALNIKKERTKFVLSQA"
BASE COUNT         2456 a         1766 c         1641 g         2775 t
ORIGIN      
        1 tatagtttgc tctttgattt tcattgagta ttagttcaat atctaaaaaa agaaccttgc
       61 caagcaagat tcttttagtg tctgacttaa ataattgttc ttctgggaac taaatcgaat
      121 taagcttcga tttctgtaac catacctgaa ccaacagtac gtccaccctc acggatagag
      181 aatgtagtac cttgttctac ggcgattggg tgaatcaact caacgtcgat tgtcacgtta
      241 tcaccaggca ttaccatttc agtacctgct ggaagttcga ttgaacctgt aacgtcagta
      301 gtacggaagt agaattgtgg acggtagttg ttgaagaatg gagtgtgacg tccaccttct
      361 tctttagtaa ggatgtagac ttcacctttg aatttagtgt gtgggttgat tgaacctggt
      421 ttagcgataa cttgtccacg ttcgatttca tcacgttgaa caccacgaag aaggacacct
      481 acgttatctc cagcaagacc ttcgtcaagt tgtttacgga acatttcaac accagtaaca
      541 actgcttttt gagtttcttc tttgatacca acgatttcga tttcgtcgtt gactttaacg
      601 ataccacggt cgatacgtcc tgaagcaact gtaccacgtc cagtgattga gaatacgtcc
      661 tcgactggaa gaagcaatgg tttgtcagtg tcacgttctg gttctgggat atactcatca
      721 actgtgttca tcaattccat aacgatgtct tcgtatttag agtcaccttc aagagcttta
      781 agtgctgaac cttggataac tggaagatcg tcacctggga agtcgtattc tgacaatagg
      841 tcacggattt ccatttcaac caattcaagc aattcttcgt cgtcaaccaa gtcaactttg
      901 ttcatgaaga cgataaggtg tttaacacca acctgacgtg aaagaaggat gtgctcacga
      961 gtttgtggca ttggtccgtc agttgaagct actacaagga tagctccgtc catttgagca
     1021 gcaccagtga tcatgttttt aacgtagtcc gcgtgtcctg gagcgtcgat gtgagcgtag
     1081 tgacgttttt cagtttcgta ctcaacgtgc gcagtgttga tagtgatacc gcgttcgcgt
     1141 tcttctggag cagcatcgat agacgcatag tctttaggtt ggttaactga tgaaggcaag
     1201 cgacgtgcca aaacagttgt gatagctgca gttagggtag ttttaccgtg gtcaacgtgt
     1261 ccgatagtac caatgttaac gtgtggttta ctacgatcgt atttttcttt tgccatttga
     1321 gtaaaagcct ccaataaaat atattttata gatagacagt aggcaataca gtctaacttt
     1381 ccttactatt ttatcaaatt tcagctaaat tgcaagtgtt ttacaacgtt tttgtgaatt
     1441 attcttggta aaaatttaac taattaatag aaagtactaa atagacttat ccttcaagga
     1501 gttttcagca ttatccatcc actcacttgc tgaaaatgta aacagtctct ggtttatata
     1561 gtaaaatgaa acaagaatag ggcaaatcga tcaggacagt caaatcgatt tctaacaatg
     1621 ttttagaagt agatgtgtac tattctagtt tcaatctact atacaacctc aaaactgtgt
     1681 tttgaacaac ctgcggctag cttcttagtt tgccctttga ttttcattga gtataaaatt
     1741 gcagataaaa cgataacaat cataggactc agcagagtca atgtaggtcg aagaatagcc
     1801 cccctaatcg cttattttat agcaaaatga aatagaaact gaacattttg ttgaggaagg
     1861 taaatcaatt cctcacaatg tttgagaaag aagggaggac tattccaact tcaacccact
     1921 ataaagcaga cttacaagat agggaaccca tctccgataa tatatagtag aatgaaaact
     1981 gcacaatttg attggagaat tcaaaccaat ttctaacaat gttttagaaa caagggtcta
     2041 ttatttcaac tgcaacctac tatagcccat tgaagctgga atagcccact gagacttcta
     2101 aaccactgct ggagaggagt ttgtttagag tttctttcat ttccctaaaa aatcaaaaag
     2161 agagaaccag tctgatcctc tcttacttat agatggtgct cactcaaatg ttaaaggagc
     2221 tatttcttag agataaagga atttgaatac agctactgcc gcaattgctg ctgcgatagg
     2281 tgctactact ggtacccaag aataccacca ttttgaatcg cctttatgct caccaagaac
     2341 tgattttgga aggaaagcat gaaggagacg tggtcccaag tcacgggctg ggttcaaggc
     2401 aggtcctgta ggtcctccaa gtgatgttac caaagccata acgaggaatc caagtgccaa
     2461 gtgagccaca gaaagtcctg aagcagtgtg tggagccacc tgtgctttaa tagccaagtc
     2521 agaaaaatca actgtttgtc ctgcttctat tgccttttgt ttcatgaatt gaagcacttc
     2581 agcaccaaag aagtttttag tcaaaccaag agctgcaaag aacaaaacaa atgaaccaac
     2641 aaactcatta atcaaaccat tgacagttgc tgcatagcga ctttcttttg taccatggtc
     2701 aatacttgaa atagttgaga aagttcccaa gatgttattt gggttttcag ttttcaagta
     2761 gaatggacgg tatgttgcca caactaaggc ttggccaaag atagccccca agacttgcgc
     2821 gataatgtaa ggtaccactt gtgcccaagg gaaaagaccg ctaactgcaa gccctagagt
     2881 gaaagcaggg ttgatgtgat tcccagatac gttaccaaac atcaaggctg ggatcataac
     2941 ccccatacca taaccaacag cgatgacgat ccagccactt tggtgacctt tcgtaccttt
     3001 aagttcaacg ttggcaactg caccattccc aagaatgatc aaaatggcag ttcccaaaaa
     3061 ttcagtggca tacttcagtg cccatgtgaa atccatttgc tagattctcc ttaaaatttt
     3121 ttagatattc cttattttat caatttttac cctatttagc aactgatttc tggaaatatt
     3181 tttaaaaaaa taggattcat agtttcccag ttcaacggac ttgtaagcgt ttttattctt
     3241 cacgaaagac ctcgttctca aaaccaggaa aaaagcccac atctgtgagc ttttccttgt
     3301 tcctagattc taaaatctat tatttttcat cttctttttt cttgaagaat cctaatccta
     3361 gaccagctaa caaggtcatg ataccagcac tagccaaggt agcattagct tctgtacctg
     3421 tgtttggcaa ttcctctctc ttacctgtct cataagtcgg aacttctggg tctggattca
     3481 ctggagtttc agtttttgga gtacctggtt ctggagttgg tttatctggt gttgataaac
     3541 ggtcatacct taccgttatt tctttatcac tagagtctga cgtaacttct tgtgattaaa
     3601 ctgttggaat atctggagct ttgtacttgt caatcttacc agatataacc tcgtcccagt
     3661 ttcctgttgt ccattcaccg taggttacaa ctcccgtgac cttgttctca gtttttgtac
     3721 ggcttaaggt tacaggttga acaacatctt cttttacatt ttggttcgta actttatcaa
     3781 cgtaatgaat gatacgcgtt atagtcttcg tctcagtaga ggttgctgtt ttgggaacca
     3841 ctgtttcctc aacattctca cggtagtaat agtcaactgt tgcaccgtct tctggtacgc
     3901 atttgcagga gttgctacca aggtgtatgt tgttttcctt gtgataactc ggtcttcttt
     3961 gtcctcagtt gttgttttcc cttcaatagt ttttgattct gtggtatact cagaacctat
     4021 cgctaaatca gcttttataa cagactctgc caacttctct tggctacctt ctttatagta
     4081 attggctgtt actgtaacag tggttggcgc ttcgctttct ctataaacta aggtcactgt
     4141 tctaccttcg cttacaatat tcccagttaa acttgcagaa tttgtatcta cttctttaaa
     4201 agtataatat tttccgtcag tagtagtcat gctactgagt tttttatctg tgacattgta
     4261 gctggtacca atcagttgtt ttttattggt aatgtaggtt ccgtcacttt ctttttctcc
     4321 aattccagta tcatttttca atgatagcaa acgcccttgt tcatcaacat agcgaacttt
     4381 cacattttct gagattagtt ctgccaattc tgaagttttt ttctttttct tgatttcttc
     4441 ggttattttc cctttctctt cttcgggaat atttagtttt ggaatgattt tttcaacaac
     4501 ggttcgtgat ggttccacag tatcttggat gactgaaaag tcagctagaa ttgggagatt
     4561 atactgaaca cggtgacttt gagtgtttac tcctactctt tcattattct ctgaaaatac
     4621 ttgtacggta taagaaacaa catcttttcc taatagaaca tccccagtag agaaatagcc
     4681 gccttttcct agtttgctat ctccagagcc cacttctttc ctaatcttat cagatagttt
     4741 tttaccagtc agtacattcg ttcgcacaat ccctttgtct acccctacaa agtgggagaa
     4801 ctttttgaac tcttcagaac cagatctagc ccaaccatta ttaagggcat ttgcttttgt
     4861 atttgtattc tctctcaaaa gtttggcgat tagaattata ttcatcggca cttagagttg
     4921 ctgctatatc tgactcttga ataccaactt ccttactacc atttctagcg gcagtatatg
     4981 tgtatttatc tgtttctact tctagctgat tatccttaac atgataggta attttctcct
     5041 ggtataagag tcttcagtag tttatatgct ctgtcatcat ctttagcttt agaagcagct
     5101 gagtttagcc aagttggggt attaggagta tctttatcag cctgtttcaa gacattattc
     5161 aagtcatttg ctagcctatt ggttgtcctc actgtgaaag ttttcttcca gtctgaacca
     5221 cttttattag acttagatat gctcatgaaa tcctggtcaa cattcaacac aatatcgcct
     5281 tgttttttag catcttccgt tgcttctgct tcaaattgat catggagttt ttgttgctct
     5341 ggtgtatagt ttttttattt acacgattta atgtactatt ttttatttta gtattaagaa
     5401 ctccattaat acgattttta aactctgctt caaaaaatcc cttaatttgt tggtgcgcaa
     5461 gttcaacatt caaacctttt ttatacaagt cttcattctg aacgatttcg gctacacggt
     5521 gaatggtttg attgtatacc gtcttctctt aaccagcagt atccttagtt ttaatgacta
     5581 ctcgataggt tcctttagaa ccgttatagt catctagttt taaaaaagca ttccaaacat
     5641 tcaaaaatag ttggaacttc ccttaataaa tccgtcactt tctttttcaa gatagcccaa
     5701 gcttgctcaa taggattcaa atccggtgaa taaggtggta gaggtaagaa aaagtgttta
     5761 tcttggatgc agagttcatc caaaatgttc ttgggatgaa aaccagcatt gcccatgaca
     5821 ataacatgag gtgtcttcaa agcaggtagg agttgcgttt tgaaccactc cacaaagaaa
     5881 tcgcttgtca tgcttttctt gtaaatcatg ggagctataa actctccgtc tacttgtcct
     5941 gcaacaattg aagttcgctc aaaacgacgt ccgctaatct tttcatagac tttctcccct
     6001 ctaggagccc ctgcataagg acgatagaga tagcggtcga ttcctgtttc atcaatataa
     6061 acaggtgcta ggtgctttaa actattaaaa ttcttaagaa ataaggctac ttttctgggt
     6121 cttgttcctt aaagctcgtc atcttttttt aaagtgacct taatctgctt taaagctgcc
     6181 catactgaag gaacagcaca atcaaaatgt gccgcaattt cccgtaaaaa agcatctgga
     6241 tgagcctcta caaaggcttt caattcctct aaaggaatct ttcggttttt gacgactcgc
     6301 tttttcctct ctatgtgttc ttgttcacgt ctttcttttc ccacgtgaag agagttctga
     6361 cgccaacacc aaaaaacttg gctgcctcga catggctgtg cccctctttg atgtaatcta
     6421 atgctcgctg tttaaaatca gtactatatg ccatagcttt attataatat gcttgtttaa
     6481 aactaagtaa ctatatcaat gttttgttat gagaagactt acttttttta ctgaaattga
     6541 atttttccta gcctcaacat catataaccc caaaattcaa tctgacctgg tttgaagaga
     6601 ttttcgaagc agataaggtt ttactaaaac taaaaaaaat gatataatca aactgattaa
     6661 atgttttaaa aataaaggag aatttgaatg tcttaccaag aaaattacca gaaatgggtt
     6721 gattttgtgg agcttcctga ctaccttcgt caagatttgg aaaatatgga cgaaaaaact
     6781 aaggaagatg ccttctatac aaatcttgaa tttggtactg caggtatgcg tggcttggtt
     6841 ggtgctggta caaaccgtat caacatctac gttgttcgcc aagctactga aggattggct
     6901 cgtttgattg agtcaaaagg tggaaacgag aaagaacgcg gtgtagcaat tgcctacgat
     6961 agccgtcact tctcacctga gtttgccttt gaatctgcgg cagttcttgc taaacacggc
     7021 atcaaatctt acgtatttga aagccttcgt ccaactccag aactatcatt tgcagttcgt
     7081 catctcaact gtttcgcagg tatcatggtc acagccagcc acaaccctgc tccatttaac
     7141 ggttacaagg tttacggtga agacggtgga caaatgcctc cacacgatgc ggacgctttg
     7201 actacttata tccgtgcaat cgaaaaccca tttgcagttg aagttgctga tgtggaaact
     7261 gaaaaagctt ctggcttgat tgaagttatc ggcgaagctg ttgacataga ataccttaaa
     7321 gaggttaagg acataaacat caacccagcc ttgattgaag aatttggtaa agacatgaag
     7381 attgtctaca caccacttca tggtactggt gaaatgttgg ctcgtcgtgc tcttgcccaa
     7441 gcaggatttg actctgttca agttgttgaa gcgcaagcaa ctgctgaccc agacttctca
     7501 actgtaacat ctccaaaccc agaaagccaa gcagcctttg cccttgctga agaacttggt
     7561 cgtcaagttg gtgcagatgt tcttgtcgca actgacccag acgctgaccg tgttggtgtt
     7621 gaagttcttc aaaaagatgg tagctacctc aacctttcag gtaaccaaat cggtgctatc
     7681 atggctaaat acatcttgga agctcacaaa aacgctggaa ctcttcctga aaatgccgct
     7741 ctctgcaaat ctatcgtttc aactgacttg gtaacgaaga ttgctgaaag ctacggcgca
     7801 actatgttca acgtcttgac aggtttcaaa tttatcgctg aaaaaatcca agaattcgaa
     7861 gaaaaacaca accacactta catgatggga tttgaagaaa gcttcggtta cttgattaaa
     7921 ccatttgtac gtgataaaga tgccatccaa gccgttcttg tcgttgctga acttgctgcc
     7981 tactaccgtt ctcgtggttt gacacttgct gacggtatcg aagaaatcta taaagaatat
     8041 ggctactacg cagaaaaaac aatctctgtt actctttcag gtgtcgatgg tgctgaacaa
     8101 atcaaagcga ttatggctaa attccgcaac aatgctccaa cagaatggaa cgcaacagct
     8161 atcactgtcg tagaagactt caaggcacaa actgctactg ttgctgacgg tactgttaca
     8221 aacttgacaa ctcctccaag tgatgtgttg aaatacacac ttgctgacgg ttcatggatt
     8281 gccgttcgcc cttcaggtac agaaccaaaa atcaagttct acattgcagt tgtaggggaa
     8341 accaacgaag aatcacaagc taagattgct aacatcgaag cagaaatcaa tgcatttgta
     8401 aaataaagac ctataaaaga tggctttttg tcaactgtag tgggttgaaa aaaagctaag
     8461 cttgagaaag gacaaatttc gtcctttctt ttttgatatt cagggcgata aaaatccgtt
     8521 ttttgaagtt ttcaaagttt cgaaaaccaa aggcgtttcg tttgataagt ttgatgagat
     8581 tattggtcgc ttccaatttt gcgttggaat aaggtaattg aagagcgttg acgatttt
//
LOCUS       DAVKGC010000086         8448 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKGC010000086 DAVKGC010000000
VERSION     DAVKGC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.086"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            143..1333
                     /locus_tag="VW049_001604"
     CDS             143..1333
                     /locus_tag="VW049_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9432691.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKTSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(1654..2535)
                     /locus_tag="VW049_001605"
     CDS             complement(1654..2535)
                     /locus_tag="VW049_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9432692.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(2676..4004)
                     /gene="vncS"
                     /locus_tag="VW049_001606"
     CDS             complement(2676..4004)
                     /gene="vncS"
                     /locus_tag="VW049_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9432693.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENSLTFFIDFPKVVQD"
     gene            complement(4001..4657)
                     /gene="vncR"
                     /locus_tag="VW049_001607"
     CDS             complement(4001..4657)
                     /gene="vncR"
                     /locus_tag="VW049_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9432694.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(4706..4789)
                     /gene="pep27"
                     /locus_tag="VW049_001608"
     CDS             complement(4706..4789)
                     /gene="pep27"
                     /locus_tag="VW049_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9432695.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(4969..6348)
                     /gene="vex3"
                     /locus_tag="VW049_001609"
     CDS             complement(4969..6348)
                     /gene="vex3"
                     /locus_tag="VW049_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9432696.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(6400..7047)
                     /gene="vex2"
                     /locus_tag="VW049_001610"
     CDS             complement(6400..7047)
                     /gene="vex2"
                     /locus_tag="VW049_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9432697.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(7060..8337)
                     /locus_tag="VW049_001611"
     CDS             complement(7060..8337)
                     /locus_tag="VW049_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9432698.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         2398 a         1846 c         1601 g         2603 t
ORIGIN      
        1 cagccactct ccagttcaat tggagagttt tttgatgaga gtagatattt atagacctca
       61 atttgacacc atacattttc tatgataaaa tgtaacaaat ttatttacaa caaatcgaaa
      121 tctaaatgaa atggaatttt ctatgaaatc agtcaaagga ctactcttta tcatagctag
      181 ttttatcttg actcttttga cttggatgaa cacttctccc caattcatga ttccaggact
      241 agctttaaca agcctatctc tgacttttat cctagccact cgtctcccac tactagaaag
      301 ctggtttcac agtttggaga aggtctacac cgtccacaaa ttcacagcct ttctctcaat
      361 catcctacta atctttcata actttagtat gggcggtttg tggggctctc gcttagctgc
      421 tcagtttggc aatcttgcca tctatatctt tgccagcatc atccttgtcg cctatttagg
      481 caaatacatc caatacgaag cttggcgatg gattcaccgc ctggtttacc tagcctatat
      541 tttaggactc tttcacatct acatgataat gggcaatcgt ctccttacat ttaatcttct
      601 aagttttctt gttggtagct atgccctttt aggcttacta gctggttttt atatcatttt
      661 tctatatcaa aagatttcct tcccctatct agggaaaatt acccatctca aacgcttaaa
      721 tcacgatact agagaaattc aaatccatct tagcagacct ttcaactatc aatcaggaca
      781 atttgccttt ctaaagattt tccaagaagg ctttgaaagt gctccgcatc ccttttctat
      841 ctcaggaggt catggtcaaa ctctttactt tactgttaaa acttcaggcg accataccaa
      901 gaatatctat gataatcttc aagccggcag caaagtaacc ctagacagag cttacggaca
      961 catgatcata gaagaaggac gagaaaatca ggtttggatt gctggaggta ttgggatcac
     1021 ccccttcatc tcttacatcc gtgaacatcc tattttagat aaacaggttc acttctacta
     1081 tagcttccgt ggagatgaaa atgcagtcta cctagattta ctccgtaact atgctcagaa
     1141 aaatcctaat tttgaactcc atctaatcga cagtacgaaa gacggctatc ttaattttga
     1201 acaaaaagaa gtgcccgaac atgcaaccgt ctatatgtgt ggtcctattt ctatgatgaa
     1261 ggcacttgcc aaacagatta agaaacaaaa tccaaaaaca gagcttattt acgaaggatt
     1321 caagttcaaa taagtaatca tactctcctg ggatttccca ggagagtttt ttatagccaa
     1381 atgaaataag acaggaacga tggaattttg gcattcaaat caattgataa gactatttta
     1441 tagtggagtg aaataggata cgaacaaatt gattaggaaa atcaaatgaa tttatagaaa
     1501 tcttttagca gttatgttat cctattctag tttcaaaacg ctatagaagc agcattgtgc
     1561 tagtctagat tcagtttact atactaaaac gagtagcttg aaatcaaaaa acccaccctc
     1621 acaggcaggt tttatctgta ttattcagct agattatgct ttaccttctg aaccgaatac
     1681 gtcgatacgt tcttcaaccg atgcttggat agcttttaca ccgtcagcca agaatttacg
     1741 tgggtcgaag agttttttct tgtcgtattc tgcttcgttt gcttcgtagt cacgagcaaa
     1801 tttacgagtt gcgttagcga atgcgatttg gcattctgtg ttaacgttaa ctttggcaac
     1861 accaagtttg atagctgctt ggatttgctc atcaggaata cctgatccac cgtgcaatac
     1921 gattgggaat cctggaagag cttctgtcaa tttttgcaag tggtcaaggt caagaccttc
     1981 ccagtttact gggtaaggac cgtggatgtt accgatacca gctgccaaga agtcgatacc
     2041 agtttcaacc attgctttag cgtcttcgat tggagccaat tcacctttac cgatgattcc
     2101 atcttcttca ccaccgatag taccaacttc agcttctact gagatacctt tagcgtgtgc
     2161 tttttcaaca acttctttag ccaatttaag gttttcttca actggaaggt gtgaaccgtc
     2221 aaacatgatt gaagtataac caacttcgat acactcaagt gcatcttcgt agtgaccgtg
     2281 gtcaaggtgg atagctactg gtacagtgat acccattgat tcaacaaggt tagcgatcaa
     2341 gttgcgagca actttgtaac cacccatgta tttagcagca cccattgaag tttggatcaa
     2401 aactggagct tttttagctt ctgctgcgcg caagatagct tgagtccact caaggttgtt
     2461 tgtgttaaat ccaccaactg cataaccgtt gtcacgggct gcttggacaa atttttctgc
     2521 tgaaacgatt gccattttat caggcctcct gtatattttt atgggtcatc ccatttacat
     2581 tgttcatttt atcacttttt gccaaaaaaa tctagttttt cccgcagttt cgattgattt
     2641 tcttctaact ccatctatgt aaaccctttc tctccctagt cttggacgac ttttggaaaa
     2701 tctataaaga aggttaaact attctcctcc atctcgaaac gataagctaa tttttcatgt
     2761 tctaatagac tcttaaccac aaagagcccc ataccagacc ccttgacctt gcgactggca
     2821 ttgtcagaaa aagactgggc tagtttttct tgttcctctg agctacagct attttcgata
     2881 aaaagttctc cttctctttc tccaattcga actaagccac ctggaacaga gtgcttaatg
     2941 gcattgctga tgagattaga aagaatcaac ttcataactg atgggtttag ataagcctgc
     3001 tgatgggtca aactattgtc tatctggagc tctctttcct tggctagcaa ggcataatct
     3061 ttgaccagat tttgcgtcat ctggaggagg tcaattgttt ccctatcatc tcgcaattcc
     3121 tgcacagaag agagggaaag tatctgcaga acatggtgat tgagttcatc cacaatcccc
     3181 aaggcaactc ccagatactg gtctctatcc ttataacgac cgatattctc tctcatattt
     3241 tcgattagga ttttcaaact agccagcggt gttttcaatt catgagaagc tcctcgtagg
     3301 aattcgacct tcatcttctc cagctggaga atggcttcat tcttttcatg caagtccgca
     3361 ataacagtca agagatgctg gtagaggcta ttgatttgtt ccttgagatt acctatctca
     3421 tccttagaat ccacgcgcaa tcgcacttgg gaatccaggt ccatcatccg acgggtcacc
     3481 cgcttgattt ccaaaatcgg tgcaacaata gtccgagcgt agatgtaggc caccaaaagg
     3541 gaaatcagaa aggaggccag caaggtatag ggaagaaact ggagactgat ttgctccgct
     3601 tccttttgta aatccatgga agctagaaac tggagaatca tagtaccacc gtcttgcgtt
     3661 ttcacctcgc gctcctcaat aaagagagag gttgtctggc ggtctgtgtc cagaggaaga
     3721 ctgtccttga cttctaactt gtcctcggtc atctcacctt tgacggtccc cttgatatca
     3781 ctagtctggg aatacaagtc taacacttgc tcgatactct gcctatcttt cccttctagg
     3841 gactgggcaa tggctgttgc cttttgacca atggtttcct gacgatgact cagataagtc
     3901 gaaggaaaaa gaaaataaat agctaaatga aggcagataa ccagaacact aaatatcgag
     3961 aaggtataga taaatatctt tgcaaataaa cctgttcgtt tcattttcgc tccaatttat
     4021 aaccaacatt gcgcacagtg aggatacaat ccaagtctag ctttttccgc aattccttga
     4081 tataaacatc aataacacgg tcaaagggaa cctcatctgt cgctttccag acggcatcga
     4141 taatctgaga tcgagtcaag gcccggcctt catttttcac tagatagtcc agaatttcca
     4201 actctttggc attgataggc acttcttgac ctgcgaggct tgcactgtag ctttcaaagt
     4261 ccaccttggt atccttgtaa gaaaagattc gtcctgtatc gtagtagcgc ttgaaaatcg
     4321 cgtccaccct cacttttaaa agggagaggg agaaaggttt ttccagatag ccatctgcca
     4381 aagaggcaaa ggcactcatc ttgtattcct catcttgaaa agctgtcaac atcaagacag
     4441 gaacctgact ggttttacga atctcagcta ggacttctaa gccgttgagc ttgggcatct
     4501 ggatatccag taaaaccagg gccacctcat agctagaaaa ttgctccaga gcttcctgac
     4561 cgtccgctgc ctcaatagtt tcatagccac aatccgtcaa ataatcactg accccctcac
     4621 ggatcatctc ttcatcttct acaattaaaa ttttcatact ttaactgctc tctatttttt
     4681 atttttctta gaataaatac ctaccctatt ttctattata gtctcttgct ggccttttgt
     4741 ctgcaagcaa ctgaccacta gataaaacgt tgtgaaattc ctttctcata aattccataa
     4801 ctttagtata ttatatttaa gcactaaagt acaaagaaag caactgaaag caatgatttt
     4861 caccactgct ttcggattta ttttgaattg ttaaatagcc attcctatcc actattcttg
     4921 aatagaaaca caagatgcaa tctttattct agactcattt tttcaaattt attcaccatc
     4981 cagcaagagc tcttttggtt gttttctaag gagattgctt gaagcaagcg ccataacgag
     5041 aaccactaga accaaggcaa ggacaaaaat gatgataaag tctgatgtct gaatggaaat
     5101 gtctaggctc gacaaggtct tgctaaagcc atctacttct gcaccaccac caaggttaga
     5161 ggcttgagcc gccttactag cctgtttggc aacacctgaa gtcacattgg caaggacagt
     5221 gtttccaatt gcacgggcag tgtaattagc taggaagtaa gcagaaacta gagcagggat
     5281 agcaatcaag atagattcgg tgatgaattg acccaagata cttgcctgct tgaggccgat
     5341 agagaggaga attcccactt ccttgcgacg ggcgttgatc caaaggctga gcaagagggc
     5401 aaggaggaga actgagaagc tcaagctacc ccagaagagg aggttggcca tcttgtacat
     5461 accagagata gattgctcaa gagctgggta gttagaggag ctcttgacga gtgtgtagct
     5521 cttccagttg ataccactga tgccattcaa ctctttcata acatcatcca agttcttgtc
     5581 tgctgttaca aagaaggttg cgtccccata aatggctgtg tcttctgtgt atccataaag
     5641 ttttgcagca gtgtgaatgt ctgtaatagc tgtgttttcg taaagttctt gtgagtaggt
     5701 tactgctgac ttattatgac catcaaagag tcccttgatt gtcacttcaa ctgtttcctt
     5761 ggctcctttt tcattatctg catcgtagat attagagtcc agtttaacct tgtcccctac
     5821 tttccagccg tgtttggctg ccaagtcctt gtgcaagagg attttatcct tgtcgtcgtt
     5881 ggttaagtgc tctccttcga ctagtttata agaaccagag acaaacttgt cttctttaga
     5941 ggagtcattg acacctgtaa tcatcaagct acttccaaaa cgcttggcac gatcagcagt
     6001 gagattcttc ttggtttctg gcgtttcaat caggtcatat ccagtcaaat ctccgatagc
     6061 gttgatacgt ttgacataag actcaatggc cttgttttcg gtgatttttt tgatgtcttc
     6121 acccttgata ttcccagcac cacgaggcgt tccttggttg acgcgacgat tgatttgcat
     6181 ggagaagcta ttggtgatat ttttaaaggt ctcctgagaa gccttggcag tagctccctt
     6241 gattgacaag ccgaccaaac tcaagctcgc catgaggaga ataatcagga agatgacaat
     6301 cgatttgaaa aacttccttg taacataggc aaatgcgttg tgtaacatag attccctttc
     6361 tagattttgt tttaatcatt ctattaaaat aagctcaaat tatttactag tattgcgcgt
     6421 ttcagtcagt ttcttatcct ttaattcaag tgtaatatct gacgcttgtg ccacttcttt
     6481 actgtgagtt acgacaatca cacatttacc tgttttctgg gcaagtgatt tgagtagttc
     6541 gacaatatct ccagcagttt taggatccag atttcctgtt ggctcatcag ctagaataac
     6601 tggagcttct gagaccaaac tgcgagcaat ggcaacacgt tgctgttgac cacctgataa
     6661 ctggagaaca ttccgcttga tctggctttc atccaaacca agctcaagaa gtgtattctt
     6721 gcttgccttt ttgttgacca atcggatatt ttccagcgga gaaagataat ctatcaagtt
     6781 ataattttga aagaccaggg aaatatggtg catgcgatgg taagaatagc ccttcttacg
     6841 aatatcctct ccttgaaaaa ggatagaacc ttcaacagga ctatctagac cagcaagtag
     6901 ggacaagagt gtggattttc ctgctcctga ctccccaata atactgtaaa attttccggg
     6961 ttcaaaatta taattgatct gatataggac tgcttcagca gtattcttat aacggtaggt
     7021 aacatcttgt aattgtaata aagtcatgat ttctccttct taactaatag atgataaaat
     7081 ttctttcggt gattttctaa ataagaatag gaaacaaagg gctacagata agcaactaag
     7141 cagaactaga aaaacatagg attctgcaaa agataagatg ctagttgata aactgcttgc
     7201 tttggctagt gtatcttgta agcttgcctg atctccactt gctagtagag tttggagtag
     7261 gtaagttgtg attgcgtttc ctgcaacaaa tgctggaagc aaagctccaa gagataccaa
     7321 aactacctct aaacagaatt gtaggaagat cgagctcttg ccttttccaa gtgcaagtaa
     7381 aatccccact tcatagaccc gttctctcaa ccagagagac aaaaccagaa ttaaggctcc
     7441 agctcctgct atcaacatcc cataaaggaa gatggtcagg aaggtttgga aagttgcaac
     7501 tgagtctttg atttgttcaa aagccttgtt ttccttttcg acttggtagc cttgattttc
     7561 caaggccaag ttttctacct gcttcatgag tccgtccatt tccttaggat tttctacata
     7621 gaagcgtgct gcactgactt gagcttcact attgcccaaa agggtttggc tactttcata
     7681 gtctgtaaag acttgatttt cactgaagtc agaagacaag cctgtgaatt tctcttgttt
     7741 tttaccagaa aagatgccga taatctcaaa ctctactgtt tgtccttttc cagattcaga
     7801 ctgaccagca tccaagccaa tcttgtcatg aagcgaaaga ccgttcttct tagccaattc
     7861 ttcgtggata aggattttct tggaatcccc tttttgaagg tgtcgccctt cttttagatt
     7921 gaaagccgaa ctggtaaagg ttacatcctt ggatgaatcc tcaagagccg ttaagctaac
     7981 caagttattg tctgcagctg ataaatcatc acgctccacg ctctgctcgc cagtcactgc
     8041 ttccttgtct tttagttttg cgaccgtctc aagttcagga gagacatttt ccagcccctt
     8101 aatcttgctt acagatgcta ggtctgacaa cttgaatgtc tgaccattct ctatcttctt
     8161 aatagaaaaa gatgtattga gtgatttata aagattgctt tctactgttt tgttggactt
     8221 catcagagtc aaacaggctg aaattccggc caataagacc aataaaatca gaaataaaat
     8281 aaaacttctc agtcgctttc tgctgacata agcccaagat ctttggattg gattcatttg
     8341 tcacctccat atttgtaaga ctattataaa acccaaatat gaaatgttta tgaaatacga
     8401 aaaaaaaata tcgagtaggg gataatctct agcccctctc acaccacc
//
LOCUS       DAVKGC010000087         8371 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKGC010000087 DAVKGC010000000
VERSION     DAVKGC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8371
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.087"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            136..1422
                     /locus_tag="VW049_001612"
     CDS             136..1422
                     /locus_tag="VW049_001612"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9432699.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGARNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            1623..2090
                     /locus_tag="VW049_001613"
     CDS             1623..2090
                     /locus_tag="VW049_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9432700.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVETGILEDECAAIMQDFFRNRRKK"
     gene            2123..2220
                     /gene="ffs"
                     /locus_tag="VW049_001614"
     ncRNA           2123..2220
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW049_001614"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2277..2720
                     /locus_tag="VW049_001615"
     CDS             2277..2720
                     /locus_tag="VW049_001615"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9432701.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            2722..3237
                     /locus_tag="VW049_001616"
     CDS             2722..3237
                     /locus_tag="VW049_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9432702.1"
                     /translation="MKIIFVRHGEPDYSELEERSYIGFGINLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            3251..4612
                     /gene="radA"
                     /locus_tag="VW049_001617"
     CDS             3251..4612
                     /gene="radA"
                     /locus_tag="VW049_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9432703.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            4685..5182
                     /locus_tag="VW049_001618"
     CDS             4685..5182
                     /locus_tag="VW049_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9432704.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            5207..5876
                     /locus_tag="VW049_001619"
                     /pseudo
     CDS             5207..5876
                     /locus_tag="VW049_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            6021..6989
                     /locus_tag="VW049_001620"
     CDS             6021..6989
                     /locus_tag="VW049_001620"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9432705.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            7107..7459
                     /locus_tag="VW049_001621"
                     /pseudo
     CDS             7107..7459
                     /locus_tag="VW049_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            7674..7790
                     /locus_tag="VW049_001622"
     CDS             7674..7790
                     /locus_tag="VW049_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9432706.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRRGQENV"
     gene            7783..8052
                     /locus_tag="VW049_001623"
     CDS             7783..8052
                     /locus_tag="VW049_001623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432707.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGIERGRAEGR
                     EQGREEGLEEGLKVGLVNLVRQGLLTPEVASEQLGMTVAEFESLL"
BASE COUNT         2345 a         1412 c         2049 g         2565 t
ORIGIN      
        1 aaatagcaaa tctttctatt gtttcttctt gataaaaagg cgatttttta ttataataaa
       61 ttgtaagata taattgcagg tgagagtcct gccatgtatg tgagaaagga agagcctgag
      121 ggctcagaca aaattatgac ttcagttgtt gttgtaggta cccaatgggg tgatgaaggt
      181 aaagggaaga ttacagactt cctttcagcg aatgcagaag taattgcacg ttaccaaggt
      241 ggtgataatg ctggtcacac gattgtgatt gacggtaaga aatttaagtt gcacttgatt
      301 ccatctggga ttttcttccc tgaaaaaata tctgtcattg ggaatggtat ggttgtaaat
      361 cctaaatctc ttgtaaaaga gttgagctat cttcatgagg aaggtgtaac aactgataac
      421 ttgcgtattt ctgatcgtgc gcatgttatt ttgccttatc atatcgagtt ggatcgcttg
      481 caagaagaag ctaagggcga caataagatt ggtacgacaa ttaagggaat tggtccagct
      541 tatatggaca aggctgctcg tgttggaatt cgtattgcag atcttttaga taaagatatt
      601 ttccgtgagc gtttagaacg taaccttgct gaaaagaatc gtctttttga aaaattgtat
      661 gacagtaaag cgattgtttt cgatgatatt tttgaagaat attacgaata tggtcaacaa
      721 atcaagaaat acgtgataga tacatctgtt atcttgaatg atgcgcttga taatggcaaa
      781 cgtgtgcttt ttgaaggtgc acaaggtgtt atgctagata tcgatcaagg tacttatcca
      841 tttgttacgt catcaaaccc tgtagctggt ggtgtgacaa ttggttctgg tatcggtcca
      901 agcaagattg acaaggttgt aggtgtatgt aaagcttata cgagtcgtgt aggagatggt
      961 cctttcccaa ctgagttgtt tgatgaagtg ggagaacgta tccgtgaagt gggtcatgaa
     1021 tatggtacaa caactggtcg tccacgtcgt gtaggttggt ttgactcagt tgtgatgcgt
     1081 catagccgtc gtgtttctgg tattactaac ctttctttga actctattga tgttttgagc
     1141 ggcttggata ctgtgaaaat ttgtgtggcc tatgatcttg acggtcaacg tattgactac
     1201 tatccagcta gtcttgagca attgaaacgt tgcaagccta tctatgaaga gttgccaggt
     1261 tggtcagaag atattaccgg agctcgcaat ttggaagatc ttcctgagaa tgcgcgtaac
     1321 tatgttcgtc gtgtgagtga attggttggc gttcgtattt ctactttctc agtaggtcct
     1381 ggtcgtgaac aaacaaatat tttagaaagt gtttggtcct aagagatttt taagatttgt
     1441 ttaagatagg tcgggtatac tatagacagt tacaagaaga cctcctaact tgttgtaaca
     1501 aatatcctaa acttttcttt ttcataataa tctccctata gagtcaccgc attcggtggc
     1561 tttttttgtg ttgggattca tgatataata ataaaatcga taagtaggaa aagagaaaag
     1621 agatgtatta tacgcttgaa gaaaaagaag tctttatgag ggaggctttg agagaggctg
     1681 agattgctct tgaacacgat gaaattccaa ttggttgtgt gattgtcaaa gatggggaaa
     1741 tcattggtcg tgggcataat gcgcgtgagg aattacagcg agcggttatg catgcggaaa
     1801 ttatggctat agaggatgcg aacttgagtg aggagagctg gcgcttgctg gattgcacac
     1861 tttttgtgac cattgaacct tgtgtcatgt gtagtggggc gattgggctt gcccgcattc
     1921 caaatgtggt ctatggggct aaaaaccaga aatttggcgc tgctggaagt ttgtacgata
     1981 tcttgacaga tgagcgtctt aaccatcgtg tagaggttga aacgggaatt ttggaagatg
     2041 aatgtgcagc tatcatgcag gactttttta gaaatagacg gaaaaaataa ttttgctttt
     2101 aaaatgaata ggaatgtgat ataataaata gtggagcaac agttctgcgt gaagcgggtc
     2161 aggggaggaa tccagcagcc ctaagcgatt tgaattgtgt gctctttttt tcgtgctttt
     2221 tccgaataaa taagatagaa taatctagaa taaatgataa tagaaaagag aaaattatga
     2281 aaattcgtgg ttttgaattg gtttcgagtt ttacagatga aaatttattg cccaagcgtg
     2341 agacagcgca tgcggctggt tacgacttaa aggttgctgt gcgtacagtt gttgcgccag
     2401 gagagattgt cttggttccg acaggggtta aggcttatat gcagccgact gaggttctct
     2461 acctctatga tcgttcttca aatcctcgta agaagggctt ggttttaatt aactcagttg
     2521 gggtcattga tggggattat tatggaaatc ctggaaatga agggcatatt tttgcgcaga
     2581 tgaagaatat cacagaccaa gaggttgttc ttgaagttgg ggagcgtatt gtccaggctg
     2641 tttttgctac tttcttaatt gcagatggag atgcagctga tggcgttcga actggtggat
     2701 ttggatcgac agggcactag aatgaagatt atctttgtac gtcatgggga gccggattac
     2761 agtgagttag aggagcgttc ttatatagga tttgggataa atttggcacc cttgtctgag
     2821 atgggacggc agcaagtcca gaaattgagc aaaaatcctt tactctcgtc agctgaaata
     2881 atcgtatctt ctgcagtcac aagagcttta gaaacggctt cgtatgtggt ctgtgctacg
     2941 ggtcttcctt taagagtaga gcctttatta catgaatggc aggtctataa aacaggaata
     3001 gaaaactttg aaacagctag aagactgttt ttagaaaaca agggggagtt gcttcctaat
     3061 agtcctattc aatatgagac agctacggaa atgaagtctc ggtttctaga atgtatgtct
     3121 aagtatcgag aacatcagac tgtggtagtt gttgctcatc gaatgctcat gcgccagttt
     3181 gtgccaaatg agaagattga tttttgccaa gtgattgagt gtgagttaga gatatagaaa
     3241 gaggtttgtc atcgcaaaga aaaaagcgac atttgtatgt caaaattgtg ggtataattc
     3301 ccctaaatat ctgggacgtt gccccaactg tgggtcttgg tcttcttttg tggaagaggt
     3361 tgaggttgcc gaggtcaaga atgcgcgtgt gtccttgaca ggtgagaaaa ccaagcccat
     3421 gaaactagct gaggtgactt ccatcaatgt caatcgaacc aagacggaga tggaggaatt
     3481 caaccgtgtg cttggaggcg gagtggtacc aggaagtctc gtcctcatcg gtggggatcc
     3541 tgggattggg aaatcaactc ttctcctaca agtctcaacc cagttgtccc aagtggggac
     3601 agttctctat gtcagtgggg aggagtctgc ccagcagatt aaactacgtg cagagcgctt
     3661 aggtgatatt gatagtgagt tttatctcta tgcagagacc aatatgcaga gtgttcgtgc
     3721 agaagtggag cgtatccagc cagactttct cattattgat tccatccaga ccatcatgtc
     3781 tcctgagatt tcaggggtgc aggggtctgt ttctcaggtg cgtgaagtga ccgctgaact
     3841 catgcagttg gccaagacca ataacattgc catctttatc gtaggtcatg tgaccaaaga
     3901 aggaaccttg gctgggcctc gtatgttgga gcatatggtg gatacggtgc tttactttga
     3961 aggggagcgt caccacacct ttcgtatttt gagagcggtc aaaaatcgtt ttggttccac
     4021 taatgagatt gggatttttg agatgcagtc gggcggcttg gttgaggtac tcaatccgag
     4081 tcaagttttc ctagaagagc gtttggatgg ggcgactggt tcctccatcg ttgtaaccat
     4141 ggaagggacg cgtccgattt tggcggaggt tcaggctttg gtaacaccga ccatgtttgg
     4201 aaatgccaag cgtactacga caggacttga ttttaaccgt gctagcttga ttatggctgt
     4261 tttggaaaaa cgggcagggc ttctcttgca aaatcaggat gcctatctca aatctgctgg
     4321 tggtgttaaa ttggatgaac ctgcgattga cttggctgtt gcagttgcta ttgcttcgag
     4381 ctacaaagac aagccaacta atcctcagga atgttttgtc ggagaactgg gcttgacagg
     4441 agagattcgg cgcgtgaatc gtattgagca acgcatcaac gaagctgcta aactgggctt
     4501 tactaagatt tatgtaccta agaattcctt gacaggaatc actctgccta aggaaattca
     4561 ggtcattggc gtgacaacga ttcaggaagt cttgaaaaag gtctttgcat aatccgtgac
     4621 aaattctctt aaaaatgata agataggaga aatatttgac tatcaaattt tcaaggaggg
     4681 aatcgtgtcg tattttgaac agtttatgca agccaatcag gcttatgttg ccctacatgg
     4741 gcagttaaat ctgccactta aacccaaaac aagagtagct attgtgacct gtatggactc
     4801 tcgtctgcac gttgcgcaag ctctgggctt ggcacttggg gatgctcata tcttgcggaa
     4861 tgcaggtggt cgagtgactg aagacatgat tcgttcgcta gttatttccc agcaacaaat
     4921 ggggacaaga gagattgtgg tattgcacca tacagactgt ggtgctcaga cctttgaaaa
     4981 tgaacctttt caggagtatt taaaagagga attaggtgta gatgtgtcag accaggactt
     5041 cttgcccttc caagatatag aagagagtgt acgcgaggat atgcaactgc ttatcgagtc
     5101 tcccctaata ccagacgatg tcattatctc tggtgccatt tacaatgttg atacaggaag
     5161 tatgacagtc gtagaattat aaatacttca tttagaaaga aagtgtatga agaaaagcag
     5221 tattttattt atttttattt tattgctatg tattggttta cagtatgaaa ccatctacta
     5281 tacggacggt tcgaggtcag gtgcggaata tggactaatg ggagtttcta tctttctagc
     5341 tctcttttac atgattccgg ctctttattt tctcttccgt attgggaaaa aatgggaatt
     5401 gccaaagaag gttttgattc tgtctttatt gggagggatg ttcctttcag gctggttgtc
     5461 tagttttgct aatacttata tccatgattt actgggggtt cttttcccag atagtacatt
     5521 tttaaatgcc tttgaaagtg ctattgtggc tcctttggta gaagaaccct tgaaattatt
     5581 gccacttgtt tttgttttgg ctttgattcc tgtgcgaaaa ttaaaatttt tgtttttact
     5641 tggaattgcc tctcactgga ctttttcagg tctagctgta gtaggtgttt acttgcttta
     5701 cagagcctat aaaggacaga aggttggcaa gaaacagggt cttattgttt aactctcctt
     5761 ttgtggagtt ggaaacagag ttgcctttag cgattccagt ggttacggct attgctctct
     5821 atggttatta tcatgcttat tgctttgttg agaaacacaa tgagttgatg acctagaata
     5881 tttttcaaaa gaatgatgca agggtacaaa tatggtgccc ttcttttatt tttgattgaa
     5941 aaatagtgca aaaagcgcta caatggtaga tggaaaatct tgtgaaaagc acaagcgata
     6001 catatatacc ggaggaaatc atgtcttttt ctgatttaaa gctgtttgcc ctttcttcta
     6061 ataaagaatt ggcagaacgt gtggcgcagg agattgggat agagttgggg aaatcaagtg
     6121 ttcgccaatt ttcagatgga gagattcagg tcaacattga agaatcaatc cgtgggaaac
     6181 acgtctttat cctacaatca actagttcgc ctgtaaatga caatctgctt gaaattttga
     6241 ttatggtaga tgctttgaag cgtgcgagtg cagaatctgt caatgttgtc atgccttact
     6301 atgggtatgc acgtcaggat agaaaggcga gagcgcgtga gccaatcact tcaaaacttg
     6361 tcgcaaatat gcttgaagta gctggagtgg atcgtttatt gaccatcgac ttgcatgctg
     6421 cgcaaattca aggattcttc gatattcctg tggatcattt gatgggtgct cctctaattg
     6481 cagattattt tgagcgtcgt ggtatggttg gttctgacta tgtggttgtc agcccagacc
     6541 atggaggggt gactcgtgct cgtaagttgg cagaattttt gaaaacatct atcgctatta
     6601 ttgataaacg tcgtagcgtt gataagatga atactagtga agttatgaac atcatcggta
     6661 aggttgaagg caagacttgt atcttgattg atgatatgat tgataccgct ggaacgattt
     6721 gtcatgcggc agatgctctt gcggaagctg gtgctgttga agtctatgca agctgtacgc
     6781 acccagttct ttctggtcct gctacggaca atatccaaaa atcagctatt aagaaattgg
     6841 ttgttttgga taccatctat ctgccagaag agcgtttgat tgataagatt gagcagattt
     6901 caatcgctca tctcctaggg gatgctatcg tacgtattca tgaaaaacga ccactttctc
     6961 cacttttcga tattgagaaa aaaatttaat gaccaagcct gagatgattc tcaggttttt
     7021 tcgtcttttt tccgaataaa tagatagtag cggtgaatct agtaaagcta gatttaaaac
     7081 tgtgatataa taaaaagaga agaaatatga ctgtacgtca tccgggcatt agcccaacca
     7141 atgacttggt agctaagaaa atctttagca atccagaaat cacttgtcaa tttattcgcg
     7201 atatgctgga tttgccagcc aaaaatgtga ccattttgga gggtagcaat attcacgtat
     7261 tactctccat gccttactcg gtgcaggatt tttataccag tatagacgtc ttgggggagt
     7321 tggataacag tactcaagta attattgaga ttcaagtcca tcatcagaat ttttcatcaa
     7381 tcgcttgtgg gcttacctgt gcagtcaggt taatcaaaat cttgaaaaaa ttcgtcagcg
     7441 agaaggtgat actcactaga gctacaaaca catcgctcct gtttacgcca ttgctatcgt
     7501 ggatagtaat tatttctcag atgacctggc ttttcatagc tttagtatgc gcgaagacac
     7561 aacaggtgag gtattggcga ttaccaacaa tggacaggaa aaccatctgg ttaagatggc
     7621 attcttggaa ttaaaaaata cagagaaacc agcaaagaca aggttcgcaa gccatggttg
     7681 gagtttttcg gcaacaagcc ctttacccag caaccgcaac gagccattac ccaagcaaat
     7741 caactgctgg actacaagag ctggtccgaa gaggacagga aaatgtttag tcaactacat
     7801 atgcgagaag aacaagtctt gttagcacag gactatgcct tggaaactgc tagggctgaa
     7861 ggccttgaac aaggactgga gcgtggtatt gaacgtggtc gtgcagaggg tcgtgaacaa
     7921 ggacgagaag aaggccttga agagggatta aaagtaggtt tagtaaatct agtacgtcaa
     7981 ggtcttctaa cacctgaggt tgcgagtgag caattaggca tgaccgtggc tgagtttgag
     8041 tccttgctat gagttcatac tttatatagt agaaagttga tgggatggca agtttattat
     8101 caaaatactt gtcatttttg cttgacattt ctatcttttt ataagatagt attagcgaag
     8161 taagttatgt ctcgctattc atacagtttg gatagtcgca agattgtctt tgagatttcc
     8221 tgttttaaag agaaaaaagc ctctttgacg ctattttttc atctttttga aagttctata
     8281 atgaagttag ccacccagcc ctccttttcc tccttctata gtgagttgaa ataagatgtg
     8341 aataactcta tcaggaaagt caaattaatt t
//
LOCUS       DAVKGC010000088         8370 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKGC010000088 DAVKGC010000000
VERSION     DAVKGC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.088"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(149..226)
                     /locus_tag="VW049_001624"
                     /pseudo
     CDS             complement(149..226)
                     /locus_tag="VW049_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            257..490
                     /locus_tag="VW049_001625"
     CDS             257..490
                     /locus_tag="VW049_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9432708.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            490..1824
                     /locus_tag="VW049_001626"
     CDS             490..1824
                     /locus_tag="VW049_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9432709.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQIFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            1834..2088
                     /locus_tag="VW049_001627"
     CDS             1834..2088
                     /locus_tag="VW049_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9432710.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            2184..2579
                     /locus_tag="VW049_001628"
     CDS             2184..2579
                     /locus_tag="VW049_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9432711.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            2862..3439
                     /locus_tag="VW049_001629"
                     /pseudo
     CDS             2862..3439
                     /locus_tag="VW049_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            3426..3986
                     /locus_tag="VW049_001630"
     CDS             3426..3986
                     /locus_tag="VW049_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9432712.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIHAYASLPEVAYPAGFL
                     PVKTLEDEIYYLVHILPNRNQKENFPAGYGIVVKGTDKVIGSVDFNHRHEDDVLEIDY
                     TLHPDYWGRGYVPEAARTLIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            3986..4564
                     /locus_tag="VW049_001631"
     CDS             3986..4564
                     /locus_tag="VW049_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9432713.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQHQPNELNH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            4557..7208
                     /locus_tag="VW049_001632"
     CDS             4557..7208
                     /locus_tag="VW049_001632"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9432714.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAQSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVVNAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            7289..>8370
                     /locus_tag="VW049_001633"
     CDS             7289..>8370
                     /locus_tag="VW049_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9432715.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDKDINVVSGDEVKEYLKIDTDDKLMLVACPPCQGFSTIGTNNENDERNQLV
                     FQFLRLINELNPDFLLMENVVGMTRTRNEKIFKKFLTSIDNNYSVIYDILNTADYGVP
                     QSRKRLVLHGIRKEILDNTNLTLNLPSKTHSNIPELDLLSWINADIIMDLPPIEGGEE
                     YIGEGIYNHVANKLSNLNIERITHIRNNGGSRTSLPKSLELNCHKNRLGHSDVYGILD
                     INKPSITITGGCMSYSKGRFGHPTQNRALSAREAARLQSFDDDYIFVGNRGQLAKQIG
                     NAVPVKLAQASGNYFMSVYREIANGQS"
BASE COUNT         2534 a         1627 c         1882 g         2327 t
ORIGIN      
        1 ttgagctgac ttcgtcagtt ctatccacaa cctcaaaacg gtgttttgag ctgacttcgt
       61 cagttctatc tacaacctca aaacggtgtt ttgagctgac ttcgtcagtt ctatctacaa
      121 cctcaaaacg gtgttttgag ctgacttcgt cagttctatc tacaacctca aaacactgtt
      181 ttgagcaacc tgcggctagc ttcctagttt gctctttgat tttcattgag tattagaaca
      241 tacaatggag gtcgtcatgg acaatatcat cgatgtgtca attcctgttg cagaagtggt
      301 ggacaagcat ccagaagtct tggaaattct agtggagttg ggttttaaac cccttgccaa
      361 tcccttaatg cgcaatacag ttggtcgtaa agtatcactt aaacagggtt ctaagctagc
      421 aggaactcct atggacaaga ttgtacgcac actggaagcg aatggctacg aagtgattgg
      481 attagactaa tgacagatga acggattcat atcctacggg atattttgtt agaattgcac
      541 aatggcgcct ctcctgagtc ggttcaagat cgctttgatg cgacctttac gggcgtgtca
      601 gccatcgaga tttcccttat ggagcacgag ctgatgaact cggattcggg cgtcactttt
      661 gaagatgtta tggaactctg tgatgtccat gccaatcttt ttaaaaatgc tatcaaaggt
      721 gtcgaagttt cagatactga gcatccaggt cacccagttc gtgtcttcaa agaagaaaat
      781 ctggctctcc gtgcggcctt gattcgcatt cgtagattgt tagataccta tgagtctatg
      841 gaagacgagg aaatgctggc ggagatgcgt aagggtttgg tgcgtcagat gggacttgtg
      901 ggtcaatttg acatccatta ccaacgtaag gaagaactct tctttcctat catggagcgc
      961 tatggacacg attcacctcc caaagttatg tggggagtgg atgatcagat tagggaactc
     1021 tttcaaacag ctctaacgac agccaagtca ctaccagaag tgtcaattag cagtgtaaag
     1081 gaagcttttg aagcttttgc gacagagttt gaaagtatga ttttcaagga agagtccatc
     1141 ctgctcatga ttctccttga gtcttttact caggatgact ggcttcagat tgcggaggag
     1201 agtgatgcct atggctatgc catcatccgt ccgtcagaga aatgggtgcc agaacgacag
     1261 atctttattg aggaaaagat tgcagaggag cctgtacaac tagatacggc agaaggtcaa
     1321 gttcaacaag tcatagatac gccagaaggc cattttacca ttacctttac ccctaaggaa
     1381 aaggaagctg tgctggaccg ccatagtcaa caggcttttg gtaatggcta tctttcagtc
     1441 gagcaggcca atctcatcct caatcatctc cctatggaga ttacctttgt caataaagaa
     1501 gatattttcc agtattacaa tgacaatacg ccagctgatg agatgatttt caaacggacg
     1561 ccgtcccaag tcgggcgcaa tgtcgaactc tgtcatccgc ctaagtattt ggacaaggtc
     1621 aaggctatca tgaagggact tcgtgagggg accaaggaca agtatgaaat gtggttcaag
     1681 tctgagtcgc gaggaaagtt tgtccacatc acctatgctg cagtacacga tgaaaacgga
     1741 gaattccaag gtgtactgga atacgttcag gatatccagc cctaccgtga gattgatacg
     1801 gactatttcc gtggattaga ataaggagaa aaaatgagtt acgaacaaga atttatgaag
     1861 gaatttgaag cttgggtcaa tacccaaatc atgattaacg acatggcgca caaggaaagt
     1921 caaaaagttt acgaagaaga ccaggacgag cgtgccaaag atgccatgat tcgctacgag
     1981 agtcgtttgg atgcttatca gttcttgctt ggtaagtttg aaaacttcaa agtaggcaag
     2041 ggattccatg atttgccaga aggcttgttt ggtgagcgaa attattaaac gagaaagatt
     2101 cttgattttt cactaaaatc ttgatagaat gtttatgtta aatccttgtc agagcaggga
     2161 ttttttattg aaaggatttt atcatgtcaa agaaactcaa tcgtaaaaaa caattacgaa
     2221 atggcctccg tcgcgcaggt gccttttcaa gtactgtgac taaggttgta gatgagacaa
     2281 aaaaagtcgt gaagcgtgca gaacagtcag caagcgcagc tggtaaggct gtttctaaaa
     2341 aagttgaaca agcagtagaa gctaccaaag agcaagctca aaaagtagct aattctgtag
     2401 aagattttgc agcaaacttg ggtggacttc cacttgatcg tgccaagact ttctatgatg
     2461 aaggaatcaa gtctgcttca gatttcaaaa actggactga aaaagaactc cttgacttga
     2521 aaggaatcgg cccagctacc atcaagaaat tgaaagaaaa tggcatcaag ttcaagtaat
     2581 ttttcttgag ccttgcattt ccgagaaaaa cttgctacaa tagagccatt agaggtgttt
     2641 tgaatcccac attttacaga aagtggcggc gctgagaagt ccacaaatgt gtcaaaactg
     2701 gttgctaatg actgaaaaaa tgaaatattt ctgtcttttt attttaaaga ctaaagatgc
     2761 gggcttggcc cgaaattggg tggtaccgcg gataaacaca ttcgtccctg tcatgtagat
     2821 ggcagggctt ttcttttgtg tcttagtcaa aggaggttgt tataaaacaa tcatttaaaa
     2881 ctaataaact atattatggt tttcctgttt ttattttagg atatcaagat cagaatttta
     2941 gctataacat catgacctgt agttcctctt atagtctagg agattgggtt gtgattggag
     3001 tcgttgcgag agagaatgcc gcagagcaga tcaaacaata tcaaaaattt actgtgaata
     3061 tttctgatga aacttctatg cttgcgatgg agcaggctgg ttttatcagt catcaggaga
     3121 aattggaacg tttgggagtg cattatgaaa tttctgaacg aactcagact cctattttag
     3181 acgcctgtcc acttgtttta gattgtcggg tagataggat tgttgaggaa gacggtattt
     3241 gccacatctt tgccaagatt cttgagcgac ttgttgcccc agaactcctg gatgaaaagg
     3301 gacattttaa aaatcaactg tttgcccaac ctatttcatg ggagatggat atcagcgcgt
     3361 ttattgctat ctggacaagc gtgtagatat gaagggcagt ttcatcaaaa aagcgagaaa
     3421 gaaggatggc aagaactgag ctgccagata aaatcgaaac agaacgtctc gttttacgag
     3481 tccgcacagt ggcggatgct gaggatatcc atgcctatgc tagtttgcca gaggtcgcct
     3541 acccagcagg ttttctgccc gtcaagacct tggaagatga gatttactat ctggtgcata
     3601 tccttcccaa tcgcaatcaa aaggaaaatt tcccggctgg ctacggaatt gtcgtcaaag
     3661 gaaccgataa agtcattggt tctgttgatt tcaaccatcg ccatgaagat gatgtgctgg
     3721 aaatcgacta taccttacac ccagactatt gggggcgagg ttatgtgcca gaagctgcgc
     3781 gtaccttgat tgacttagcc tttaaagatt tgggtcttca caagattgaa ctaacttgct
     3841 ttggatataa ccttcaaagt aaacgagtcg cggaaaagct tggctttacc ctcgaagctc
     3901 gcataagaga ccgcaaagat gctcaaggca actgctgtga tgatttaaga tatgccttgc
     3961 tgaagagtga atgggaggtg atttgatgca tcttttcatc attggtgctc cagcatcagg
     4021 aaaaatgacc attggtcaag aattatctcg actgacggat gctaccctat tttataacca
     4081 tcaagccatc gattttgcac tagaaatcta tcaggactat acagaggaaa tgtgggaatt
     4141 tgttcgtgga atcacttttt cattccttgg agcgagtgca agaaatcagc gatcagtaat
     4201 tttaacagat gtaattgatt tttcaaatca gtatcacctg atatatttaa agcaaattca
     4261 gagtttgttg gatgattatc atcaagagat tctttttgtt gagttggaaa caagtcttga
     4321 agagtgcata cgtcgaaatc gaacggagaa tcgattaaag tacaaaccct taaaacgaca
     4381 tattgaggta tctgaaagag aaattataga gaccgctgaa acacttcaat taaattccca
     4441 acatcaaccg aatgagttga accactactt taaaataaat aatacgaatt tgtctgcaga
     4501 agaagctgct aagcagattc aaaataaaat aaacaaaata gagaaaggac acacacatgt
     4561 ctaaagaact ttcacctaaa tacaatccag ccgaggttga ggctggtcgt taccaaaaat
     4621 ggcttgatgc cgatgttttc aagccttcag gcgatcaaaa ggctaagcct tattcaatcg
     4681 tgattccacc accaaacgta actgggaaac tccaccttgg tcacgcttgg gatacaactt
     4741 tgcaagatat tatcatccgt caaaaacgca tgcaaggttt tgataccctt tggcttcctg
     4801 ggatggacca cgcagggatt gccactcagg ctaaggttga ggagcgcttg cgtggtgagg
     4861 gcatttcccg ctatgacctt ggtcgtgagt ctttcttgac gaaagtctgg gaatggaaag
     4921 acgaatatgc cactaccatc aaggaacaat ggggcaagat ggggctctct gtagactatt
     4981 ctcgtgagcg tttcactctt gacgaaggtt tgtcaaaagc tgttcgtaag gtctttgtga
     5041 acctttacaa gaaaggctgg atctaccgtg gtgagtttat catcaactgg gacccagcag
     5101 ctcgcacagc cctttctgat attgaggtga ttcacaagga tgtggaaggt gccttctacc
     5161 acatgaatta catgctggaa gatggttcac gcgcccttga agttgctaca actcgtcctg
     5221 agactatgtt tggggacgtt gcggttgcgg ttaatccaga agacccgcgc tacaaggact
     5281 tgattggtaa aaacgtcatc cttccaatcg ctaataaact catcccaatc gttggagatg
     5341 agcacgcaga tcctgagttt ggtactggtg tcgtgaaaat cacacctgcc cacgatccaa
     5401 atgacttctt ggttggccaa cgtcataact tgccacaagt caacgtcatg aacgacgacg
     5461 gaactatgaa tgagcttgcc tttgaatttt caggcatgga tcgttttgaa gctcgtaagg
     5521 cagtcgttgc taagttggaa gaaatcggtg ccctcgtcaa aatcgaaaaa cgtgtccaca
     5581 gtgttggtca ctcagagcgt acaggtgttg tggttgaacc tcgcttgtct actcaatggt
     5641 tcgtcaagat ggaccaattg gctaagaacg ccattgccaa ccaagacaca gaggacaagg
     5701 tcgaattcta cccacctcgt ttcaacgata ccttccttca atggatggaa aatgtccacg
     5761 actgggttat ctctcgtcag ctctggtggg gtcaccaaat ccctgcctgg tacaatgctg
     5821 atggtgaaat gtatgtcggc gaagaagctc cagaaggtga cggatggact caggacgaag
     5881 acgtcttgga tacttggttc agttctgccc tctggccatt ttcaaccatg ggctggcctg
     5941 aagtcgactc agaagacttt aaacgttatt tcccaacttc aaccttggta acaggttacg
     6001 acatcatctt cttctgggtg tctcgtatga tcttccagtc cttggaattt actggtcgtc
     6061 agccattcca aaacgtgctt atccacggtc tcatccgtga cgagcaagga cgcaagatgt
     6121 ctaaatctct cggtaacggg attgacccaa tggatgtcat cgagaaatac ggtgctgatg
     6181 cccttcgttg gttcctttca aacggttccg caccaggtca agacgttcgt ttctcttacg
     6241 agaaaatgga tgcttcatgg aactttatta acaaaatttg gaacatttct cgctatatcc
     6301 tcatgaacaa tgaaggtttg acgctggatg tggcgcatga caatgtcaca aaagttgcaa
     6361 caggtgaggc tggtaatgtg acggaccgct ggattctcca caatctcaac gaaaccattg
     6421 caaaagttac tgaaaacttt gataagttcg agtttggtgt ggctggtcat atcctttaca
     6481 acttcatctg ggaagaattt gccaactggt atgttgagtt gaccaaggaa gttctataca
     6541 gcgacaatga agacgataag gtcattactc gatctgttct cctctatacg ctggacaaga
     6601 tccttcgtct ccttcaccca atcatgccat tcgtgacaga ggaaatcttt ggacaaatct
     6661 cagaaggttc tatcgtgaca gctgcatacc caactgttaa tctagccttt gaagaccttg
     6721 cggctcacac tggtgtggaa agtctcaaag acttgattcg tgctgttcgg aatgcgcgtg
     6781 cggaagtaaa cgtagcacaa agcaaaccta tcaccatcct tgttaagaca agcgatagcg
     6841 acttggaagc cttctttaac agcaatgtca actatatcaa acgctttaca aatccagaac
     6901 acttggaaat cgcatcaacc atccctgcac ctgaactggc tatgtcaagt gtcatcacag
     6961 gagcagaaat ctacctgcca ctggccgatc tcctcaatgt cgaagaagaa ttggcacgtc
     7021 tggaaaaaga acttgccaaa tggcagaaag aactggacat ggttggtaag aaactctcta
     7081 acgaacgctt cgtagtcaac gccaaaccag aagttgtcca aaaagaacgc gacaaacaag
     7141 ctgactacca agccaagtat gatgcgaccg tagcacgtat tgatgagatg aagaagttgg
     7201 tgaaatgatg ttaaaaacct aaccctatcg ggttgggttt tacatttgat aatgttataa
     7261 taaaaaggag aaactttagg agaatactat ggggaaaatt gttgctattg atttatttag
     7321 cggtgcaggt ggcactacaa gtggccttaa aaaatcaggc atagatgttc aagttgctgt
     7381 tgaaattgat tccgtagctg ttaaaactta taagttaaat aatcctgagg tgtctgtaat
     7441 agataaagat ataaatgtag tatcaggaga tgaagtaaaa gagtatctta aaattgatac
     7501 agacgataaa ctaatgctcg tggcatgccc accatgtcaa ggtttttcaa ctattggtac
     7561 taataatgaa aatgacgaga ggaatcaact tgtttttcaa tttcttcgtt tgattaatga
     7621 attgaatcct gattttctat taatggaaaa tgttgtgggt atgactagaa caagaaatga
     7681 aaaaatcttt aaaaaatttt tgacatcaat cgataataat tatagtgtta tttatgacat
     7741 tttaaataca gctgattatg gagttcctca atctagaaaa agattggtgt tacacggaat
     7801 tagaaaagaa attcttgata acacgaattt aactttgaat ttgccttcca aaactcattc
     7861 aaatatacca gaattggacc ttctttcatg gattaatgcg gatataataa tggacttgcc
     7921 tcctattgaa ggtggtgaag aatatattgg agaaggtatc tacaatcatg ttgcaaacaa
     7981 actatctaat ttaaatattg agagaattac tcatattaga aataatgggg gaagtagaac
     8041 ctctttgcca aaatctttag aattaaattg tcataaaaat cgtttaggtc atagtgacgt
     8101 atatggaata ttagatatca acaaaccttc cattactatt acaggagggt gtatgtcata
     8161 ttcaaaaggt agatttgggc acccaactca aaatagagct ttaagtgcaa gagaagctgc
     8221 aagattacag tcttttgatg atgactatat ttttgttgga aatagaggtc aattagctaa
     8281 gcaaattggt aatgcagttc cagtaaaatt agctcaagct agtggaaatt attttatgtc
     8341 agtttatagg gagatagcaa atggacaatc
//
LOCUS       DAVKGC010000089         8006 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKGC010000089 DAVKGC010000000
VERSION     DAVKGC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.089"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            19..1446
                     /gene="galT"
                     /locus_tag="VW049_001634"
     CDS             19..1446
                     /gene="galT"
                     /locus_tag="VW049_001634"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9432716.1"
                     /translation="MYNEIDKIYVKNRVLALIGEEGTDRISDENDLKQIKDYLVEIAL
                     KNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISPQEAVEEFYKLSKDSDY
                     IKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKLVKATNYPKCLLCMENE
                     GYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIFLNSQHVPMAITSKTFE
                     QLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPMEKAKFESEFRFKDFED
                     VNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEVDIIAMTEDVPHHTVTP
                     IARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKENIGLIEVMGLAILPPRL
                     KPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVNSVVQHEAGQVFARVLE
                     DAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1459..2439
                     /gene="galE"
                     /locus_tag="VW049_001635"
     CDS             1459..2439
                     /gene="galE"
                     /locus_tag="VW049_001635"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9432717.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKPALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWN"
     gene            2649..3308
                     /locus_tag="VW049_001636"
     CDS             2649..3308
                     /locus_tag="VW049_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9432718.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3333..4400
                     /locus_tag="VW049_001637"
     CDS             3333..4400
                     /locus_tag="VW049_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9432719.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKVLDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4504..5514
                     /locus_tag="VW049_001638"
     CDS             4504..5514
                     /locus_tag="VW049_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432720.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5526..7217
                     /locus_tag="VW049_001639"
     CDS             5526..7217
                     /locus_tag="VW049_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9432721.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7220..7930
                     /locus_tag="VW049_001640"
     CDS             7220..7930
                     /locus_tag="VW049_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9432722.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2694 a         1128 c         1581 g         2603 t
ORIGIN      
        1 agagtaattg aatacggaat gtataatgaa attgacaaaa tatatgtgaa aaatagagta
       61 ctagcgttaa taggtgagga gggaactgat cgcatttccg atgaaaatga cctgaaacaa
      121 ataaaagatt acttagtgga aatcgcctta aaaaacggta aaattaaaga tttaatcgag
      181 gaaaaggaat gtttaggagc agagttaatg aatttcatag ttcctttacc tagtcgatta
      241 aatgatattt tttggagttc atatgatata tcacctcaag aagcagttga agagttttat
      301 aagttaagta aagatagtga ttatattaaa acttctgcta ttgctaagaa tattgagttt
      361 cgagcatcaa ctaaatacgg agagctagag attacgataa atttgtcaaa acccgaaaaa
      421 gatccaaaaa caattgctgc tgaaaaatta gtaaaagcca ctaattatcc caaatgttta
      481 ctatgtatgg aaaatgaagg atatcagggg cgaataaatt atccagcacg ttcgaatcat
      541 agaattattc gattgaaact tggtgacgaa gtatggggat tccaatattc tccctactta
      601 tactttaatg agcatgcgat atttttaaat agtcaacatg taccaatggc tattacctct
      661 aaaacatttg aacaattatt ggagattgtt gatattttgc caggatattt tgctggttca
      721 aattctgatc ttcctatctc aggaggctcc attcttagtc ataaccacta ccagggaggg
      781 aaacatattt tcccaatgga aaaagcaaaa tttgagagtg aatttcgttt taaagacttt
      841 gaggatgtta atgctggtat tgtaaaatgg ccaatgtcag taattaggtt acaaagtgaa
      901 aataaaaatc gcttgttaga tttagctact aaaattctaa ataagtggag agaatattca
      961 gatttagaag tagacatcat tgctatgact gaagatgtcc cacaccatac ggtgactcct
     1021 attgctcgta aagtggacgg gagatatgaa ttagatattg ttctaagaga taatcatact
     1081 actgaacagt acccagatgg tgttttccat cctcatcaag atgtacaaca tattaaaaag
     1141 gaaaatatcg ggttaatcga agtgatggga cttgcgatat taccacctcg tttgaaacca
     1201 gagttagaag aagttggaaa atatctttta ggagaggata acgcaattgc ggattatcat
     1261 ttagaatggg ccgaccaatt gaaagaaaaa tatcctcgca ttaataaaga agaggttaat
     1321 agcgtagttc aacacgaagc aggacaagtg tttgcgagag ttctggaaga tgcaggagtt
     1381 tataagaata caccgtctgg gcatgaagcg tttatgagat ttgttaagtc ggttggaatt
     1441 aattaggagg aaacaaaaat ggcaatattg gtaacaggcg gagctggtta tattggtagc
     1501 cataccgtag tagaattact aaatttagga aaggaagtca tcattgtcga caacctttcg
     1561 aactctagca tcttggtgct agaccgtatt gaagcaatta caggaatacg tcccgtgttt
     1621 tacgaattag atgtttgtga taaaccagca ttgagaaagg tatttgaaca agaatcgatt
     1681 gatgctgcaa ttcattttgc aggttataaa gctgtcggag aatccgtgca aaagcctgtg
     1741 atgtactaca aaaataatat tatgagtaca ttggcacttg ttgaagtgat gtcagaattt
     1801 aatgttaaaa agattgtctt ttcttcaagt gcgactgtat atggaattaa caatcagtca
     1861 cctctaattg agactatgca aacaagtgcg acaaatcctt atgggtatac gaaagtgatg
     1921 cttgagcaaa ttttaaaaga tgttcatgtg gcagattcag aatggagtat tgcgttgctt
     1981 cgttatttca atccaattgg tgctcatgag tctggtttga ttggggaaga tccctcagga
     2041 attcctaaca acttgatgcc ctatattgca caagtagcgg taggtaagct gtcagaacta
     2101 agtgtgttcg gaaatgatta tgatacgctt gacggtactg gtgtgcgcga ttatatccat
     2161 gtagtagatt tagcaatagg acatataaaa gctttagaaa aagtatctga aaaaacagat
     2221 gtttatatct ataacctagg ctcgggagaa ggcactagtg tattacaact tgtaaataca
     2281 tttgaaagtg ttaataagat ccctattcct tataaaatag ttccaagacg ttcaggagac
     2341 gttgcaactt gttatgcaaa tgcagacaaa gcatataagg aattaaattg gaggacaaca
     2401 aaatcgattg aagacatgtg tagagataca tggaattagc aatcaaaaaa tcccaatggc
     2461 tataattgat gatactgtct atatgagtgc tgtattttgg aaaaattact taacctttta
     2521 atgaaatgca tatttaatga aagataaatt aaaaaaaatg tttgacagcg cttgacaaag
     2581 gaaacggttt cattgtataa tgtaaacata gtgacaccga tgtcaaatat aaaaaagaag
     2641 gtctttatat gaaaaactta acaaaaatta agttcaaaga gaatggggaa tttaatcatt
     2701 tccctgggaa tacagttgta gcaaatcttt atactaaaca agatttgatg gaagttgttg
     2761 atattattca atcacgttat agagaattac catttatcga taagtttact ttaactccaa
     2821 ggaattctat tcacatgact gtaattgaat tgttgtgcca tgaaaatcgt gaaacggagt
     2881 tttggagcag taatcttcct ctagatacac ctttacagga aatacatgat tactttgcta
     2941 aacaacttga aatttttcca ttgttggatg aagaaattca tatgcgtata actgaaatgg
     3001 gaaaacaaaa catactagtt gaacctgcag atgaagcttc tgcaaagaga ttagaagaaa
     3061 ttcgtactta tgtctcagaa aaagcaggtg tttgtttccc taatcatgat agatatcaat
     3121 tccatatttc aattgggtat cttcggattc ctctaaccga agaggaggaa gaagagttta
     3181 ctaaagtcag agcagaatta actgaaattt tattagagaa gattccaact attactgtaa
     3241 accgtattga ctatactgta tttgaagata tgagacaatt tgttccatat cacgaaaaat
     3301 ttaaataaaa taataaaaaa ggagaactta tcatgaaaaa aatgaaagtt tggtctactg
     3361 tacttgcaac gggagttgct cttactacac ttgctgcttg ctctggaggt tcaaattcta
     3421 cgactgcttc ttcatctgaa gaaaaagctg ataaaagtca agaattagta atctattcga
     3481 actcagtctc aaatggtcgt ggtgattggt taactgctaa agcaaaagaa gctggtttta
     3541 atataaaaat ggttgatatt gctggcgctc aattagcaga ccgtgttatt gctgagaaga
     3601 ataatgcagt tgcagatatg gtatttggaa ttggtgctgt tgactcaaat aaaattagag
     3661 atcaaaaatt actagtacag tacaagccta aatggttaga taaaattgat caatctttat
     3721 cagataaaga taattattat aatcctgtga ttgttcaacc attagtttta attggggcgc
     3781 ctgatgtaaa agaaatgcct aaagattgga ctgaattagg tagtaagtat aaaggtaaat
     3841 attcaattta tggtcttcaa ggtggtacag gacgggcaat tctagcaagt atcttagttc
     3901 gttaccttga tgaaaaagga gatttaggcg tatcagaaaa aggatgggaa gttgcaaaag
     3961 aatactttgc aaatgcctat actcttcaaa aaggtgaaag ctcagttgtt aaagtgttag
     4021 ataaagaaag cccaattcaa tacggtatga tctggggttc tggagcgtta atcggacaaa
     4081 aagaacaaaa tgttgaattc aaagttatga ctcctgagat tggtgtacca tttgtaactg
     4141 aacaaactat ggttttaagc actagtaaaa aacaagcgtt agctaaagaa tttattgatt
     4201 ggtttggtca atcagaaatt caagtagaat atagtaaaaa atttggatct attcctgcca
     4261 ataaagatgc cctaaaagag ttacctgaag atacgaaaaa atttgttgat caagtgaaac
     4321 cacaaaatat tgactgggaa gctgttggaa agcatttgga tgaatgggta gaaaaagctg
     4381 aattagaata cgtaaaataa gaaataagag ttatttttta tgtccaagtc tctctattat
     4441 ttctaaattt aaggaagaaa taaagagagg cttttggttt tagtaataga gggagttagt
     4501 gagatgatta aatttgataa tattcaaatt aaatatggtg attttgttgc aattgataat
     4561 ctgaatttag atatacatga aggggaattc tttacatttc ttgggccttc aggatgtggt
     4621 aaatcaacta ctttgagagc gttggtaggt tttttagatc catcatcagg aagtattgaa
     4681 gttaatggaa cagatgtcac tcatttggaa cctgaaaagc gtggaattgg tattgtattt
     4741 caatcttatg cgctatttcc aactatgact gtttttgata atattgcatt tggtttaaaa
     4801 gttaagaagg tagctccaga tgttattaaa gctaaagtat cagcagtggc agcaaaaatt
     4861 aagatctctg atcaacagtt acagcgtaat gtatcagaat tatctggggg tcaacaacaa
     4921 agggtagcat tggctcgtgc tctggttctt gaacctaaaa ttctttgtct agatgaacca
     4981 ttgtcaaacc ttgacgcaaa attacgtgta gatttgagaa aagagttgaa aagacttcaa
     5041 aaagagttag gtattactac tttatatgtt actcatgatc aagaggaagc cttgacttta
     5101 tctgatagaa ttgcagtctt taacaatgga tacatcgaac aggtcggtac accagtagag
     5161 atttatcata attctcaaac tgaatttgta tgtgatttta ttggagatat taatgttttg
     5221 accgatgaaa cagtccacga agtattattg aaaaatacaa gcgttttttt agaggataaa
     5281 aaaggataca ttcgattaga gaaagttcga ttcaatcgtg aaactgaaca agattttatt
     5341 ctaaaaggga caattattga tgttgagttt tctggagtta caattcacta tacaataaaa
     5401 gtttctgaaa gtcagattct taatgtaaca agtattgata gtcaggctgc tattagatct
     5461 gtcggagaaa gtgtggaatt atttatcaca ccatcagacg ttctgcaatt ttaaggaggt
     5521 gcagtatgcg tcataaatta aatttaaaag attggcttat tcgtttaggg ttaatctggt
     5581 tcttagtaac atttattatt tatccaaact ttgatctagt agtgaatgta tttgtaaaag
     5641 gaggagaatt ttcccttgat gctgtacatc gtgttctaaa atctcagagg gcacttcaga
     5701 gtattatgaa cagttttaag ttagcatttt cactcattat tacagttaat gtcgtaggta
     5761 ttctttgtgt tctattcaca gagtactttg atattaaagg tgctaaaatt ttaaaattag
     5821 gttatatgac ctctttaatt tatggaggag tggttttagc gactggatat aaatttgtct
     5881 atggtcctta tggattgatt acaaaatttt tacaaaatgt tatcccttct ttagactcta
     5941 actggtttat tgggtatggt gcagtcttat tcattatgac attttcagga actgctaatc
     6001 atacattgtt tttaacaaat acaattcgaa gcgttgacta tcacactatt gaggctgctc
     6061 gaaatatggg agcaaaacca tttactgttt tccgaaaagt agtgttacca accttaattc
     6121 caactctatt tgcacttact attatggttt ttcttagtgg tttatctgca gtagcagcac
     6181 ccatgattgt tggtggtaaa gaatttcaaa ctataaatcc aatgattatt acatttgcag
     6241 ggatggggaa ttctcgtgat ttagctgcct tacttgcaat tattttaggt attgcaacta
     6301 caattttgct tactatcatg aataagatag aaaaaggtgg aaattatatt tctatctcta
     6361 agactaaagc gcctcttaaa aaacaaaaaa ttgcgtctaa gccttggaat atcattgctc
     6421 acattgtagc atatggattg ttcacagttt tcatgcttcc actaattttt atagtattat
     6481 actcatttac agatccagtt gcaattcaaa caggtaactt aacattatca aactttactt
     6541 tagaaaatta tcgcttattc tttagtaata gtgcggcatt ctctccattc ttggtcagct
     6601 ttatttattc tattattgct gcgacaacag caacaattct cgcagttgta tttgctcgtg
     6661 ttgtcagaaa acataaatct cgttttgatt tcttatttga atatggtgct ctacttcctt
     6721 ggttactacc aagtacactt ttagcagtaa gtttattatt tacttttaat cagccacaat
     6781 ttcttgtctt gaatcagatt ttggtaggta gtttggtaat tctacttatt gcatatatag
     6841 ttgtaaaaat cccattttct tatagaatgg tacgtgctat tttatttagt gttgatgatg
     6901 agatggaaga tgcagcaaga agtatgggtg cttcaccttt ttatactatg atgaaggtta
     6961 tcattccatt tattttaccg gttgttctct ctgttattgc tttaaacttt aactctttat
     7021 taactgactt cgacttatct gtattccttt accatcccct agctcaacca ttaggtatta
     7081 cgattcgatc tgcaggtgat gaaacagcaa catctaatgc acaagctctg gtatttgttt
     7141 atacaattgt tctgatgatt atttctggaa cggtattata cttcacacaa agaccggggc
     7201 gtaaagtaag gaaataatca tgacagccac tagtcttggg ttatcaaata ttgaaatagt
     7261 tgtcaggatt gttttatcag tagtcattgg tagtataatt ggtttagaga gagggagcaa
     7321 atcccagcct gcaggcatcc gaacttatag tattgtttgt ctagctgcat gtttgattat
     7381 gatgacgaat gaatacgtat cttataaatt tgggacagga gatcctacac gattaggagc
     7441 tcaagttata tcaggtgtgg gttttctagg cgctggaacg attcttatta cagataaaaa
     7501 gaaaattaca ggtctgacaa ctgcagcagg catttgggct tcggcaggaa ttggattagc
     7561 tattggagta ggtttttatg agggagctct tttagtagcc atttctgttt ggggtgtgat
     7621 atccatgttc caaccactaa aaaaatatct gcaaaatcgt tctaaaatga ttgaattgta
     7681 tatagtagtt aaatctacag aagcctataa tcgtgtattg gtttattgtg cagaaaatgg
     7741 tatcagaatg accgattcaa gaacagcgtt tggagatgtt aattctgaca gaattgaata
     7801 ctttgatgtt ccggacaaaa aaatagcgtc atttattact ctggaattat caggtagatt
     7861 tgaacatctt cgactgatgg aagaaattgc taatattgta ggtgttattt atgtcgaaga
     7921 aattagctga aatttagtaa acaaagggac agatgttttt gtccttttgt tttttagatg
     7981 ttttaatcat ctaatttttg agtaga
//
LOCUS       DAVKGC010000090         8006 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKGC010000090 DAVKGC010000000
VERSION     DAVKGC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.090"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            90..1151
                     /gene="dinB"
                     /locus_tag="VW049_001641"
     CDS             90..1151
                     /gene="dinB"
                     /locus_tag="VW049_001641"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9432723.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            complement(1298..2143)
                     /locus_tag="VW049_001642"
     CDS             complement(1298..2143)
                     /locus_tag="VW049_001642"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9432724.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            2398..3368
                     /locus_tag="VW049_001643"
                     /pseudo
     CDS             2398..3368
                     /locus_tag="VW049_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3567..5456)
                     /locus_tag="VW049_001644"
     CDS             complement(3567..5456)
                     /locus_tag="VW049_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9432725.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            5594..7159
                     /locus_tag="VW049_001645"
     CDS             5594..7159
                     /locus_tag="VW049_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9432726.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQITGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMVQVEPGF
                     KTGEEDTAIAIGLRKDDTRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            7159..7923
                     /locus_tag="VW049_001646"
     CDS             7159..7923
                     /locus_tag="VW049_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9432727.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
BASE COUNT         2532 a         1763 c         1525 g         2186 t
ORIGIN      
        1 cgcagattgc tcaaaacact gttttgaggt tgcagataga gctgacgtgg tttgaagaga
       61 ttttcgaaga gtataaaaag gtgctaggca tgttgatttt tcctttgtta aatgatttgt
      121 caagaaaaat catccatatt gacatggatg ccttttttgc tgcggtggaa atcaaggata
      181 atcctaaact cagaggaaaa cctgtcatta ttggaagcga ccctcggcaa acaggtggac
      241 ggggagtcgt ttctacctgt agctatgagg ctcgagcttt tggtgtccat tctgccatga
      301 gttccaagga agcttatgaa cgttgtcccc aggccgtctt tatctcagga aattatgaga
      361 aatacaaagc tgtgggactc cagattcgag ctatttttaa gcgctataca gatttgattg
      421 aacccatgag cattgacgaa gcctatttgg atgtgacaga aaataaactc ggtatcaagt
      481 cagcggtcaa aattgctcgc ctcattcaaa aagatatctg gcaagaactc catctaactg
      541 cttctgccgg tatttcctac aacaagttct tagctaaaat ggcgagtgat tatcaaaaac
      601 ctcatggttt gacagtgatt ctacctgaac aggctgagaa ttttctcaaa caaatggata
      661 tttccaaatt tcatggagta ggaaaaaaga cagtagaacg tcttcatcaa atgggcgttt
      721 ttactggtgc tgatttactt gaagttcctg aggtgaccct aatagaccgt tttggtagac
      781 taggctatga tctgtatcga aaggctcgtg gcattcacaa ctctccagtc aaatccaatc
      841 gcatccgtaa atcaatcggc aaggagaaaa cctacgggaa gattctccgt gctgaggaag
      901 atatcaaaaa agagctgact cttctatcag aaaaagtcgc tctcaatcta catcaacaag
      961 aaaaagctgg aaaaattgtc attttgaaaa tccgctacga ggacttttca actcttacca
     1021 aacgaaaaag tcttgctcaa aaaacacaag atgctagtca gataagccaa atagccctgc
     1081 aactctatga agaattaagc gagaaagaaa gaggtgtccg cctattgggg attaccctga
     1141 ctggatttta aagctcaatg aaaatcaaag agcaaactag gaagctagcc gcaggctgct
     1201 caaaacactg ttttgaggtt gcagataaag ctgacgcggt ttgaagagat tttctaagag
     1261 tataaaacct tgaagagttg ccccttcaag gtttttctta tacaaataaa cggacaaaac
     1321 tgtaaagtag caaaacacta ccaagtacga tacggtattt accaaaaagg gtgaagtcgt
     1381 gttttttcac atagctggtc aagaagcgaa tagccaccat gctgaccgca aaagctactc
     1441 ccatcgcgac caagagcaaa aacaattgcc caaagctcaa gagttctccg gctttcacaa
     1501 atttgaaaat ctttaaggca ctagctccaa acataacggg aatcccaaga tagaaggtaa
     1561 attctgtcac aactgaacga ctggttccat ttaacaaacc accgacaatc gttgcacctg
     1621 aacggctagt ccctggtaaa agagcaagaa cttggaagag tccgatatag aaagcggtcg
     1681 tataaggaag cttgtccaac tctgttacac ttggctcgat agcacgcgct ttattgcgct
     1741 tttccaaata gatgaaggca accccgtaga taatcaacat gagagcaact gaaaccatgt
     1801 tatggaagtg ggtatcaaac caatcatcaa atttaaagac accaagtaaa ggtaaagtag
     1861 caatcaagac cttcaaccat agtctccaag tcttacgaac ttcctgtttg tccttagtcg
     1921 gtttaaaagg attgagcttg ttaaaataaa tcaccataac tgctaaaata gcaccaagct
     1981 gaatcacgac attaaacatg gacataaagg cttcattttg attttggtat tggatgaatt
     2041 cctctgctaa aatcaagtga cctgtactgg aaatcggcaa ccattccgta attccttcaa
     2101 caatcccgaa gaagatagat tttaaaattt caataagata catagattac tccctatatt
     2161 ataattagcg cagttagcta ctgccaaagc ccaagtggta tacttggaat aagccactgt
     2221 ggattagttc atttcctcca atcgcttgac gatttccgaa aggctctaac cacagctttt
     2281 gtttcaattg gtaatgagtt tgataacgca tagacgtttt atctcgtcta aggtaacaag
     2341 gcaaaaagtt cgtggataac caagtagact tgaaatacta tatctggagg tactcccatg
     2401 ctttatgtgg gcattgatat cgctaaaaat aaacacgatg ttacagcctt gaatgttcca
     2461 ggaaaaactg ttcttaaacc actcactttt tcaaataata aagctggttt tgaactctta
     2521 gatctgtctc ttcgacagct caaccaagac tgtctcatcg ctcttaaact tctttctgac
     2581 cccaatcgtg aacaatttca acacgataat cggcaagtag aactaaaaat actggctaga
     2641 tatattcatc gtctcaagaa aaaacagtct gattggaaag tacaatacac tcgttgtctt
     2701 gatatcatct ttcctgagtt ggataaaatc gttggaaagc attcagaata tacctaccaa
     2761 ctcttgacac gctaccctaa tcctcagaaa aggattgagg caggatttga taagctgata
     2821 gaaattaagc attgaccgct tctaaaattc aggatatcct ctcagtcgca cctcgttcta
     2881 tcggaacaac atctcctgct cgtgaattcg aaatcatcga aatcatcaaa cattacaaga
     2941 gactcattga caaggcggaa acatgtgtca atgacttgat ggctgagttc aactcggtca
     3001 tcacgacggt tactgggatt gggaatcgtt taggggcggt cattttagcc gagattcgaa
     3061 atattcatgc ctttgataat cctgctcaat tacaagcttt cgctggactg gattcttcta
     3121 tttatcagtc aggtcagatt gatttagctg gaagaatgat caaacggggt tcccctcatc
     3181 tgcggtgggc actcatacaa gctgccaaag catgcgctcg cttttcacct gcttttaagg
     3241 cctatcttaa gactaagtta gaacaaggaa aacattacaa tgtagccatc atccaccttg
     3301 caaaaaaact tatccgaacc ctgttttata tcctaaaaaa gagctgccat ttgacgaaca
     3361 aaaagtgatg taatttctga aattaacaca ggattacctt tgaaaaaatg gactttttca
     3421 agggcttttt gccgtgctct aaaatcatcg ccaaatcatc aaattgaaat aagaaatagt
     3481 ttagataaaa ctattgactt ttcttatagt ttgtctttct tctatcttcc attatatcat
     3541 actttttcag tctatggtag ctctctttaa aaggctccga tactgccgcc acctccgcct
     3601 ccagagaagc caccgccaga acttccactt ccagaagata cggagtaggt acttgctgta
     3661 tttgcgacac tagcataatg gctcatttgc gcgcttgaat gataaaacat actgtgccag
     3721 ccataagcta catagagatt gatatctgga ttttcaactt gaatctgatg aaccttcatc
     3781 aaatgactaa ccttgtccgc atagccaaat aaggttgcat agaccaagag acgattccaa
     3841 acaacaatac tttccaattc agccttatcc agtcgtgcaa tctcacgtaa catattttca
     3901 aaactggtcc agagatagta gacctcagct cccgcttcat ttagaacacc atcacgatta
     3961 tctagtcgaa gcttccaata atagaaaaca gacaaaacta accctagaaa accaagtatt
     4021 ggcaaaggga ggtaaagata gccatgaacg tctaaactgt acaagaacaa accaaatccg
     4081 ataaataggg gcaagatagt caagacaccc atacccactt gcaaagtctt ttccaaacca
     4141 gttaaaggac gatagtaatc tgggagcccc cagaaggaaa ctcgttttct cactccttct
     4201 tgcatctggt tcaatacctc ttcaaaagaa gatttgagtt gaagccctct tgcttgaatc
     4261 cgtttttcat cagaaacttt ggctctacga taaagactat cagatacctt gtaatccgca
     4321 aacaaattgg aaagagtggc ttcttttttg cctgaaaagg ctagatttag acagtctctc
     4381 tcaaatcttg acaaaccatc ttctttcacc aatctcaagc caactgcatc tccttctgaa
     4441 ataatagaga cattcccacg gtctatcaca tctagcaagg tagcttgaat aagttgatca
     4501 aaggtgaatt ttccagctcc cttgaccaag ggactcactt cctccaagga ggtcgagtag
     4561 actgcttctg ataaaaccat aggctctaat tccattggtg gttcatagag acgatgattt
     4621 ttggcatatt tgactgaagg agtggtcttt cttctataaa taaaatagaa gcagacactc
     4681 aataacaagg agatagaaag gatcgaaggg aggacccaag taacgagttg tttactctga
     4741 tctttttctc taacaatcga gtcttctatc ttattaaact cttctaaacg attccctttc
     4801 aagccctgat ccctagcgct agcaaaatcg gtccgagacc aataggcatg caactcaact
     4861 ccacgcttag ccggaagatt gtctaaacgg atagtataat caaggttact cttttcaatc
     4921 gttccctctc taaaaagttt ccctgtatgg aaaaagagtt tttcagcccc cttgtctccc
     4981 cttacatgaa attcaaactt ttcaatagac tctgaactat ctgtcagagg ttgccaattt
     5041 aattcagcga tatcatcata aaggaaaagt aaatttttta agttccagac gaggtcaact
     5101 tcaactatgt cgccctcctg acctggatta tagactctca cagtataacc atccgcttct
     5161 tctgttactt cgctagtcac atctgctagt tctgcaccgt ttttcgcagc ctgaaccttt
     5221 ggatgagggt caatgtcaaa cccgctaggc atcttaccag cacgtccaag tcccacgatt
     5281 tggcccttaa agtcctcctc aaactggtaa actatcttct gtctaaactc tgccgtatta
     5341 tctgcatgaa tatacaaatc cccttgataa gagtttatct tgaaatcaat ggcaaaaaca
     5401 gagagtggaa gaaggcaaaa caagcctaac accagtaaga aaaaagtttt tttcatcaac
     5461 taagccccct ttttatcttt cttatcatta tagcattttt tcgtaagtaa aggcttactt
     5521 atgttgaaaa attacgcatt tttcgataaa ataggagaca gttatttttt aatagatcag
     5581 aataggagaa atcatgagaa aaatatactt atctattttc acaagtctct tgctgatgct
     5641 aggacttgtc aatgttgctc aagccgatga atatttacgc atcgggatgg aagcagcata
     5701 tgctcccttt aactggaccc aggatgatga tagcaacgga gctgtcaaaa tcgatgggac
     5761 caatcagtat gccaacggat acgatgttca aatcgccaag aaaatcgcta aggacttagg
     5821 taaagaacct ttggttgtta aaaccaagtg ggaaggtcta gtccctgccc ttacttctgg
     5881 taagattgac atgattatcg caggtatgag tccaactgca gaacgcaaac aagaaattgc
     5941 cttttcgagc agttactaca ctagcgaacc agttctactg gtcaaaaaag attctgccta
     6001 tgcaagtgct aaatctttgg atgactttaa tggtgcgaaa atcacttctc aacaaggggt
     6061 ctacctttat gacttgattg cacaaatcac aggtgctaaa aaagaaacag ccatgggaga
     6121 cttcgctcaa atgcgccaag ctcttgaggc tggtgtcatc gacgcctatg tttctgaacg
     6181 cccagaagct ctgactgccg aagccgctaa ctctaagttc aagatggtcc aagtagaacc
     6241 aggtttcaaa actggggaag aagatacagc tatcgccatt ggacttcgta aagatgacac
     6301 tcgtattagc caaatcaatg ccagcattga aaccatttca aaggacgacc aagttgccct
     6361 gatggatcgt atgatcaagg aacaacctgc cgaagctaca acaactgaag agactagcag
     6421 tagtttcttt agccaagtcg ctaaaattct ttctgaaaac tggcaacaac tcttgcgtgg
     6481 tgctggtatc actcttttaa tctctatcgt cggaaccatc ataggtctca ttattggact
     6541 tgccattggt gttttccgta ctgctcctct ctctgaaaac aaagccattt atggccttca
     6601 aaaactagtc ggctgggttc tcaatgtcta cattgaaatt ttccgtggta ccccaatgat
     6661 cgttcaatcg atggttatct actatggaac tgcccaagct ttcgggatca accttgaccg
     6721 tacactggct gctatcttca tcgtttcaat caataccggt gcctacatga ctgaaatcgt
     6781 ccgtggtggt atcctagcag ttgacaaggg acaatttgaa gctgcgactg ctcttggtat
     6841 gacccataac cagaccatgc gtaagattgt cctacctcag gtagtccgta acatcctacc
     6901 tgcaactggt aatgaatttg tcatcaatat caaagataca tctgtattga acgttatctc
     6961 tgttgtcgaa ctttatttct caggaaatac cgtggcaaca caaacctatc aatacttcca
     7021 gacatttaca atcatcgccg tgatttactt tgtcctcacc ttcaccgtaa cacgtatcct
     7081 acgatttatc gagcgccgaa tggacatgga tacctatact acaggtgcta accaaatgca
     7141 aacggaggat ttgaaataat gacacaagca atccttgaaa ttaaacacct caaaaaatcc
     7201 tatggacaaa acgaagtgct aaaagacatt tcactcactg tccacaaggg agaggtcatc
     7261 tctatcatcg gaagctctgg aagcggaaaa tcgaccttcc tacgctccat taacctactt
     7321 gaaacaccaa ctgatggaca aatcctttat catggacaaa acgtcctcga aaaaggctat
     7381 gacctcacgc aataccgtga aaagttgggg atggttttcc aatcctttaa cctctttgaa
     7441 aatctcaatg ttcttgaaaa cacaatcgtc gctcagacaa ctgtcctaaa acgcgaacgc
     7501 acagaagctg aagaaattgc caaagaaaac ctggaaaagg tcggcatggg agaacgctac
     7561 tggcaagcga aacccaaaca actctcaggt ggtcaaaaac aacgtgtggc cattgctcgt
     7621 gccctttcaa tgaatcctga cgccattctc tttgatgaac caacatcagc tctcgatcca
     7681 gaaatggttg gagaggtcct caaaatcatg caagatctgg ctcaggaagg cttgaccatg
     7741 attgtcgtaa cccatgaaat ggaatttgcc cgtgatgtct ctcaccgtgt tatctttatg
     7801 gataagggtg tgattgctga agaaggtaaa ccagaagacc tcttcactaa tcctaaagaa
     7861 gaccgtacaa aagaatttct tcaacgttat ctcatactca atgaaaatca aagagcaaac
     7921 taggcagcta gccgcaggtt gctcaaaaca ctgttttgag gttgcagata gagctgacgt
     7981 ggtttgaaga gattttcgaa gagtat
//
LOCUS       DAVKGC010000091         7989 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKGC010000091 DAVKGC010000000
VERSION     DAVKGC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.091"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            108..2741
                     /gene="polA"
                     /locus_tag="VW049_001647"
     CDS             108..2741
                     /gene="polA"
                     /locus_tag="VW049_001647"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9432728.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDY
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2826..3263
                     /locus_tag="VW049_001648"
     CDS             2826..3263
                     /locus_tag="VW049_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9432729.1"
                     /translation="MSQEVINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(3589..4599)
                     /locus_tag="VW049_001649"
     CDS             complement(3589..4599)
                     /locus_tag="VW049_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU9432730.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            4748..5917
                     /locus_tag="VW049_001650"
     CDS             4748..5917
                     /locus_tag="VW049_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9432731.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            5914..6684
                     /gene="recO"
                     /locus_tag="VW049_001651"
     CDS             5914..6684
                     /gene="recO"
                     /locus_tag="VW049_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9432732.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            6681..7673
                     /gene="plsX"
                     /locus_tag="VW049_001652"
     CDS             6681..7673
                     /gene="plsX"
                     /locus_tag="VW049_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9432733.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLVADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            7679..7912
                     /locus_tag="VW049_001653"
     CDS             7679..7912
                     /locus_tag="VW049_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9432734.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
BASE COUNT         2261 a         1468 c         2057 g         2203 t
ORIGIN      
        1 tttactaaat tctctggctc tatctattta ttcggaaaaa gtttgaaaaa tacttgctct
       61 agctcttccc aatggtattt tttgattctt tcctttataa tgggtgtatg gataagaaaa
      121 aattattatt gattgatggg tcttctgtag cttttcgggc gttttttgcg ctgtatcagc
      181 agttggaccg ttttaagaat gcggctggtt tgcataccaa tgcgatttat ggttttcagt
      241 tgatgttgag tcatttgttg gagcgggttg agccgagtca tattttggtg gcttttgatg
      301 cgggaaagac gaccttccgg acagagatgt atgcggacta taagggtggt cgggccaaga
      361 ctcctgatga gtttcgtgag caatttcctt tcattcgtga gttgctggat tatatgggga
      421 ttcgtcacta tgagctggct cagtatgagg cggatgacat cattgggacg ctggataagc
      481 tagcagagca ggatggtttt gatattacca ttgtcagtgg ggacaaggat ttgattcagc
      541 tgacggatga gcatacggtg gttgaaattt ccaagaaagg tgtggctgag tttgaggcct
      601 ttacgccaga ttacctcatg gaagaaatgg gcctcacacc agctcagttt atcgatctca
      661 aggcgctcat gggtgataag tcggataata tccctggggt gaccaaagtc ggtgaaaaga
      721 cgggtattaa gctcttgctg gagcatggtt cgcttgaggg gatttatgaa aatattgatg
      781 gaatgaagac ttctaagatg aaggaaaatc tcatcaatga caaggaacag gcctttttgt
      841 ctaaaacact agcgaccatt gataccaagg caccgattgc gattggttta gaggacttgg
      901 tctatagtgg tccagatgtt gaaaatcttg ggaaatttta cgatgagatg ggcttcaaac
      961 agctcaagca ggctttaaat gtgtcgtcag ctgatgtgtc tgagagtttg gattttacta
     1021 ttgttgacca aatcagtcaa gatatgctga gtgaagagtc tatcttccac tttgagcttt
     1081 ttggtgagaa ttaccatacg gataatttgg ttggatttgc ctggtcttgt ggggataagc
     1141 tctatgccac agacaagctt gagctgttgc aagacccgat tttcaaggat ttcttagaaa
     1201 aaacatctct gagagtttat gactttaaga aggttaaagt tcttttgcaa cgttttggtg
     1261 tggatttgca ggcgcctgct tttgacatcc gtttggctaa atacctcctt tcgactgtgg
     1321 aggacaatga aattgcgacc atcgctagtc tttatggtca gacttacttg gttgatgatg
     1381 aaactttcta cggtaagggt gttaaaaagg ccattcctga acgtgagaaa ttcttggaac
     1441 atttagcttg taaacttgct gttttggtag aaacagagcc tattttactt gaaaaactca
     1501 gcgaaaatgg gcaattagag cttctttatg atatggagca acctctggct tttgttcttg
     1561 ccaagatgga aattgctggg attgtggtca agaaagagac cttgcttgag atgcaggctg
     1621 aaaatgagct tgtcattgaa aaactgactc aagagattta cgagctggct ggtgaggagt
     1681 ttaatgtcaa ctcgcctaag cagttgggcg tgcttctctt tgagaaattg ggacttcctc
     1741 tagaatacac taagaaaacc aagacaggtt attcgacagc agtggatgtg ttggagcgtc
     1801 tcgctcctat tgctccgatt gttaagaaaa tcctggatta ccgtcaaatt gctaagattc
     1861 aatctactta tgtaattggc ttgcaggact ggattttggc tgatggaaag attcatactc
     1921 gctatatgca ggatttgacc cagaccgggc gtttgtctag tgtggatcca aacttgcaaa
     1981 atattcctgc ccgattggaa caagggcgct tgattcggaa ggcttttgtg ccagagtggg
     2041 aggatagtgt gctactcagc tctgactatt cacagattga attgcgcgtt ttggcgcata
     2101 tttctaagga tgagcacttg attaaggcct tccaagaggg ggcagatatc catacttcga
     2161 cagccatgcg ggtctttggc attgagcgtc ctgataatgt gactgcaaat gaccgtcgca
     2221 atgccaaggc agttaacttt ggagtggttt atgggatttc agactttggc ttgtctaata
     2281 atttgggaat tagtcgtaag gaagccaaag cctacattga tacctacttt gaacgttttc
     2341 caggtattaa aaactacatg gatgaagtgg tgcgggaggc gcgtgataag ggctatgtag
     2401 agaccctctt taagcgtcgc cgtgagttgc cagatatcaa ttcgcgcaac ttcaatattc
     2461 gtggttttgc ggagcgaact gctatcaact cacctatcca gggttcggca gcagatattc
     2521 tcaagattgc catgattcag ctggataaag ccttggttgc aggtggttat cagactaaga
     2581 tgctgttaca agtgcacgat gaaatcgtcc ttgaagtgcc taaatctgaa ttggtagaga
     2641 tgaaaaaatt ggtgaaacaa accatggaag aagccattca actcagtgtt cctcttatcg
     2701 cagatgagaa tgaaggggca acctggtacg aggctaaata aaaagggggc tagtcctcct
     2761 tttttgtagt agaattctgc aaaccttttc aaaatgtgct atactgatga aaaaggagga
     2821 tttctatgag tcaagaagtt atcaatccaa gtgatggcgt gattcgtcag tatctcgcaa
     2881 cgagtaaaac ccttgctgtg gtgggtttgt ctgaccgtga agaaacaacc agcaatcgag
     2941 tgacaaagga aatgcaggct cggggctata aaatcatccc agtcaatccc aaggcggcag
     3001 gtggcgaaat cttgggtgaa aaggcttatg ctagcctcgc tgaaattcct tttcctgtag
     3061 atattgtcaa tgtttatcgt cgcagtgaat ttctgcccga tgtggcgcgt gattttctca
     3121 aagctgatgc caagattttt tgggcacagc taggacttga aagtttagaa gcgaaagaga
     3181 ttttgcgtga tggtggatgt gatgatatcg tgatgaatcg ctgtatcaag agagaacata
     3241 ctcgcttgat tgaggaagca taagaaaaag gtagctgatg ggctaccttt tgtgttatac
     3301 tcaatgaaaa tcaaagagca cactagcaaa ctaggaaact agccgcgggc tgctcaaaac
     3361 accgttttga ggttgtagat acgactgacg aagtcagctc aaaacaccgt tttgaggttg
     3421 cagatagaac tgacgaagtc agctcaaaac accgttttga ggttgcagat agaactgacg
     3481 aagtcagctc aaaacaccgt tttgaggttg cagatagaac tgacgaagtc agtaacatat
     3541 atacggcaag gcgacgttga cgtggtttga agagattttc gaagagtgtt agaaaatgcc
     3601 gataagggtc tgcataccaa gagtggtgag gatgatggca atccagcaga tggctccaag
     3661 aagaatggat tttccactgg atttgaccat agcgaccaga ttggttttga gaccgatagc
     3721 actcatggcc atgacaataa ggaatttaga gagttgtttg agaggggtaa agaaactact
     3781 ggacacacct agagaggtga gtagtgtagt gaggagagag gcaaggataa agtaaaggat
     3841 aaaaagtggg aagacttttt tcagttgtag gctttgcttg ttttcttgtt ggcgactttg
     3901 ccagtaggat agaaagagcg tgatagggat aatggccaaa gtacgtgtga gtttaacaat
     3961 ggttgcagac tcgagggtat tgctttggta aagactgtcc caagcgctgg cggcagccgt
     4021 tacagaggaa gtgtcgttga ccgcagtccc tgcaaagagg gcgaagcctt cattggatag
     4081 atgaagccag gtgccgaggg ttggaaagat gagcgcagcc aagacattga agaaaaagat
     4141 aacggaaatg gcttgggcta cttccttttc cttagcatgg ataacaggcg ctgtcgctgc
     4201 aatggcagaa cccccacaga tagaagaacc tactccaacc aaggtagcca gttttgtatc
     4261 cagtgcaaag aaacgctgga agaggtaggc aataatcaga gctattgaga tagtggacag
     4321 gatgacaggg agtgaagatt ggccaactgc gaagacctgc gagatattga gaccaaaacc
     4381 aagcaagaca acggcatatt ggagcaactt cttggaacta aaggtcaaac cagcatccag
     4441 ttgtttatag gacgagagaa agggatgtag gagcatgcct atgaaaatgg caaaaacggg
     4501 cgcgccaatc acagggaaga atcctcctaa gtaccaagat aggatggaaa tgagaaggca
     4561 ggccaagata cctgctccat tttttgatag aaatgacata aaaacctcca aataaaatct
     4621 attaccatta tagacctata aacaggaaaa gtaaaataga aagtggaaag ctattctcta
     4681 atgtattttt gcggtcgggg ggcttttgta gtataataga gatacgtttt gaaagtagga
     4741 ggtatctatg gacttaacta agcgctttaa taaacagtta gataaaattc aagtttcgtt
     4801 gattcgtcag tttgaccagg ttatttcgga gattcctggg gtcttgcgtt tgaccttggg
     4861 ggaacctgat tttacaacgc cagaccatgt caaggaggcg gccaagcgag cgattgatca
     4921 gaaccaatcc tactatacag ggatgagtgg tctgctgact ctacgtcagg cagccagtga
     4981 ctttgttaag gaaaagtacc aactggacta tgctcctgaa aatgaaatct tggttacaat
     5041 tggggcgaca gaggctttat ctgcgacttt gacggctatt ttggaagagg gagacaaggt
     5101 acttttgcca gctcctgctt atccaggcta tgaaccgatt gttaacttag ttggggcaga
     5161 aattgttgag attgatacga ctgaaaatgg ttttgtcttg actcctgaga tgttggagaa
     5221 ggccattttg gagcagggtg ataagctcaa ggcggttatt ctcaactatc cagccaatcc
     5281 gacaggaatt acctacagtc gagagcagtt agaggccttg gcagctgttt tacgcaagta
     5341 cgaaattttt gttgtctgtg atgaggttta ctcagaattg acctacacag gcgaagccca
     5401 tgtgtctcta ggaacgatgt tgagagacca ggctattatt atcaatggtt tgtctaaatc
     5461 gcatgccatg acaggttggc gtttggggct gattttcgct cctgcgacct tcacagccca
     5521 gttaatcaag agtcaccagt acttggtcac tgccgcaaat accatggcgc aacatgctgc
     5581 ggtagaagcc ttgacggctg gtaaaaacga tgcggagccc atgaagaagg aatatatcca
     5641 acgtcgggac tatatcatcg aaaaaatgac tgctcttggt tttgagatta tcaaaccaga
     5701 cggtgccttc tatatttttg ctaaaattcc agcgggctac aatcaagact cctttgcttt
     5761 tctgaaggat tttgctcaga agaaggccgt tgcctttatc cctggtgcag cctttggacg
     5821 ttacggggaa ggctacgtcc gcctatctta tgcagccagc atggagacta tcaaagaagc
     5881 catgaaacga cttgaggagt acatgagaga agcatgattc agtctatcac gagtcaaggc
     5941 ttggtgcttt acaatcgcaa ttttcgtgag gatgacaagc tcgtcaaaat ttttacagag
     6001 caggttggca aacgcatgtt ttttgtcaaa cacgctggtc agtctaagct ggcgcctgtt
     6061 attcagccct tggtgctggc acgatttctc ttgcgaatca atgatgacgg actcagttac
     6121 atcgaagact atcatgaggt catgactttt cccaagatta atagtgacct ctttgtcatg
     6181 gcctatgcga cctatgtggc agctcttgca gatgctagtt tgcaggacaa tcagcaggat
     6241 gctcccttgt ttgctttttt gcaaaagact ttggagttga tggaagcagg cttggattat
     6301 caggttttga ccaatatttt tgaaattcaa attttgactc gatttggaat cagcctcaat
     6361 tttaatgagt gtgtcttctg ccatcgggtt ggtcaggctt ttgacttttc tttcaaatat
     6421 ggagcctgcc tctgtccaga gcattatcat gaggataaga gacgttgtca tctcaatccc
     6481 aatatcccct atctgctcaa tcaatttcaa gctattgatt ttgagacttt ggagaccatt
     6541 tcgctcaagc ctggaatcaa gcaagagcta cgccaattta tggatcaatt atatgaagag
     6601 tacgttggga ttcacctaaa atcaaagaaa tttattgatt ccctagcaga ctggggacaa
     6661 ttactaaaag aggaaaagaa atgaaaaaaa tcgcagtaga tgccatgggg ggcgattacg
     6721 cacctcaggc cattgttgag ggtgtcaatc aagccctatc tgacttttca gatatcgagg
     6781 ttcaacttta cggagatgaa gctaaaatca agcaatatct gacagcgaca gagcgcgtca
     6841 gcattatcca tacggatgag aagattgatt cggatgatga acctacgaga gctattagga
     6901 ataagaaaaa tgccagtatg gtattggcag ccaaggctgt caaagatggt gaagcagacg
     6961 ctgtcctttc ggctgggaat acaggtgcct tgttggctgc tggattcttc atcgtgggtc
     7021 gtatcaagaa tatcgaccgt cctgggctta tgtcgacttt gccgactgta gatgggaagg
     7081 gatttgacat gctagacctc ggtgccaatg cggaaaatac agcccagcac ctccatcaat
     7141 acgcggttct aggatccttc tatgctaaaa atgttcgtgg cattgcgcaa ccacgtgttg
     7201 gcttgctcaa taacggaaca gagagtagca agggcgatcc gcttcgtaag gaaacttatg
     7261 aattactagt ggctgatgaa agtttgaact ttatcggaaa tgtggaagcg cgtgatttga
     7321 tgaatggcgt tgcagatgtt gttgtggcag atggtttcac gggaaacgct gtgctcaaat
     7381 ccatcgaagg gacagctatg ggaatcatgg gcttgctcaa gacagctatt acaggtggtg
     7441 gtcttcgagc gaaactaggt gccctccttc tcaaggacag cctcagaggt ttgaaaaaac
     7501 agctcaatta ttcagatgtt ggtggagcgg tcttgtttgg tgttaaggca cctgttgtca
     7561 agactcatgg ctcaagcgat gccaaggctg tttatagtac gattcgccag attcgtacca
     7621 tgctagaaac agacgtagtt gcccagactg cgcgtgaatt ttcaggagaa taaaagagat
     7681 gacagaaaaa gaaatttttg accgtattgt gaccattatc caagagcgac agggagagga
     7741 ctttgtcgtg acagaatcct tgagtctgaa agacgatttg gatgcggatt ctgttgactt
     7801 gatggagttt atcttgactc tggaagatga atttagtatc gaaatcagcg atgaagaaat
     7861 tgaccaactc caaaacgtag gagatgtggt taaaatcatt caaggaaaat agcaatcgga
     7921 gttccaagtc aacggaagta gatggttttt agaaatgaga aatatcggac aagctggtaa
     7981 aatcttggc
//
LOCUS       DAVKGC010000092         7955 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKGC010000092 DAVKGC010000000
VERSION     DAVKGC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.092"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..1474
                     /locus_tag="VW049_001654"
     CDS             <1..1474
                     /locus_tag="VW049_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9432735.1"
                     /translation="LLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKDDLS
                     HVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAES
                     ICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYY
                     FLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDK
                     DSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESLFMK
                     CVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEFILF
                     DQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHLVIN
                     LLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSPYDI
                     IISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1836..2837
                     /locus_tag="VW049_001655"
     CDS             1836..2837
                     /locus_tag="VW049_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9432736.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            2988..3905
                     /locus_tag="VW049_001656"
     CDS             2988..3905
                     /locus_tag="VW049_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9432737.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            3916..4806
                     /locus_tag="VW049_001657"
     CDS             3916..4806
                     /locus_tag="VW049_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9432738.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4835..6451
                     /locus_tag="VW049_001658"
     CDS             4835..6451
                     /locus_tag="VW049_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9432739.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVENLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6461..7780
                     /locus_tag="VW049_001659"
     CDS             6461..7780
                     /locus_tag="VW049_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9432740.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
BASE COUNT         2671 a         1256 c         1511 g         2517 t
ORIGIN      
        1 tttattatct aaaaaaagtc ataggcaatt agaattatta gaattattat ttgaacataa
       61 acgttggttt catcgttctg aactagcaga gttattaaat tgtacagaac gtgcagtcaa
      121 agatgatcta tcccatgtta aatctgcttt tcctgacttg atttttcatt cttctactaa
      181 tggtatacgc attattaata ccgatgatag tgatattgaa atggtttacc atcatttctt
      241 taaacattca actcattttt cgattttaga attcatcttc tttaatgaag gttgtcaagc
      301 tgagagtatt tgtaaagaat tttatatcag ttcatcttcg ctctatcgta ttattagcca
      361 aatcaataaa gtgattaaaa ggcaatttca atttgaagtc agtctgaccc ctgttcaaat
      421 cattggaaat gagagagaca ttcgttactt ttttgcacaa tatttttcag aaaaatatta
      481 tttcctagaa tggccatttg aaaatttttc atcagagcca ctatctcaat tgttagaatt
      541 ggtttataag gaaacaagct ttccaatgaa tttgtcaacg catagaatgc taaagttgct
      601 cctagttacg aacctatata gaataaagtt tggtcatttt atggaagtag ataaagattc
      661 ttttaacgac caaagtttgg attttttaat gcaggcagaa ggaatagaag gtgttgctca
      721 gagttttgaa tcagaataca atatctcttt agatgaagaa gttgtttgcc aattatttgt
      781 gtcttacttt caaaaaatgt ttttcataga tgaaagtctc tttatgaaat gcgtaaaaaa
      841 ggatagttat gttgaaaaat cttaccatct attgagtgat tttattgatc agatttcagt
      901 caagtatcag attgaaatgg agaataagga taatctgatt tggcatctgc ataataccgc
      961 acatctgtat cgtcaagagt tgtttactga gtttatttta tttgatcaaa aagggaatac
     1021 aatcaggaac tttcaaaata tttttccaaa atttgtttca gatataaaaa aagagctttc
     1081 tcactattta gagactcttg aggtttgttc tagttcaatg atggtaaatc atctatctta
     1141 taccttcatc actcatacca aacatttggt gattaatttg ctacaaaatc agcctaaact
     1201 aaaggtttta gttatgagta attttgatca gtatcatgca aaattcgttg cagagacact
     1261 ttcttattac tgtagcaata attttgaact ggaagtttgg actgaattag aattatcaaa
     1321 ggaatcttta gaagattcac cttatgatat cattatttct aattttatta ttcctcctat
     1381 tgaaaataag agactcatct attcaaataa tataaacacg gtctcactca tatacttgtt
     1441 aaatgccatg atgtttattc gattagatga gtaaccaaaa ataacaaaaa aacgctattt
     1501 cgcgtttgaa ctgtacctca aaagttagac agaaaaaatc taatttttgg ggtgtttttg
     1561 ttaagaaaag accttcaatc atttaagcaa aatgtacatc tagatttata gccctctaaa
     1621 actcttgctt tttttgtaga agatacgaaa aaagccataa aaccaaggct tttcctgttt
     1681 tatgtagatg tttacacatg aaaattgaag tattttcaaa agctaattgg agtgatttta
     1741 ttcatgatgg gataaatgat ctggccaaaa atgattgaga aatagttgct taaaaagcat
     1801 ttgcatgata ctatatattg aaaggaggtg ataatatggt agaacaaaga aaatcaatta
     1861 ccatgaaaga tgttgcttta gaagcaggag ttagtgttgg aactgtttca cgtgtaatta
     1921 ataaagaaaa aggcattaaa gaagtaactt tgaaaaaagt ggaacaagcg attaaaactt
     1981 tgaattacat tccagattac tatgctagag gaatgaaaaa aaatcgaaca gaaacgattg
     2041 caatcattgt accaagtatc tggcatccct tcttttcaga atttgctatg catgtggaaa
     2101 atgaagtcta taagagaaat aacaaattac tcttatgttc tataaatggt acaaatagag
     2161 agcaagacta tctggagatg ttgcgtcata ataaagttga tggagtggtt gccattacct
     2221 atagtccaat tgaacattac ttgacgtcag gaattccctt tgttagtatt gaccgcacat
     2281 actcagatat tgccattcct tgtgtttcat cagataatga tgcaggtgga agagaagcag
     2341 ctaaacaatt aattagtaaa ggatgtcagc atttagcctt tgtgggcgga cataatacaa
     2401 ccattaatga aaccaaacgt cgtagaattt catttgaaaa gtatgtccaa gagcataaaa
     2461 tacctcttag tatttttgat ttggatgaga cagttactga ctattatgga aagctagaga
     2521 gtttcttgga agaaaattct tctatagatg gaatttttac aatcaacgat tttacagctt
     2581 tggatgtgat tgaaatacta gaaaaaaatg gcaaacgtat tcctcatgat gtccaaatta
     2641 ttggatacga tggaattaaa atggctggag atagagatta tctactttca actattgaac
     2701 aaccattgga agaaatggca aaagaagcgg ttcgtatttt gtttgatata attgatggaa
     2761 agactgttaa tttgcagaca atcctaccag taaaatttgt tgaaggaaaa acaacaaaaa
     2821 atgaaaataa gtcttgacag aaagcgctat caatgataga atgaattcag ataaaaagat
     2881 ttatttttaa aacaaaaatg aaacgtttca aaaaaagaaa taaaaagaca gcgccaagcg
     2941 ctatctttta tagaaaaaat gaaacgtttc aaaaaaagga ggttgctatg aatagcaaag
     3001 cgaagcaagt ttctctttgg gaaagaatca agaaacaaaa actcttgtta ttgatgactg
     3061 tccccggttt agttttaacc tttatcttta aatacatccc tatgtatggg gttttaatcg
     3121 catttaaaga ttacaatcct ttaaaaggaa ttttagggag tgattggatt ggtttttctg
     3181 agtttacaaa attcatatcc tctcccaact ttggtatctt gttagccaac acattaaaat
     3241 taagtatcta tggtttattg cttggctttt taccaccaat cattcttgcg attatgctca
     3301 atcaactctt gagtgaaaaa gtcaaaaaac gaattcagct cattttatac gcaccaaact
     3361 ttatctcagt cgttgttatt gtcggtatga ttttcctctt cttttcagtg ggaggaccaa
     3421 tcaacaattt tctttctatg tttggaatga aggctgactt cttgacaaat ccagacttct
     3481 ttagaccctt atacatcttt agtggtatct ggcaaggaat gggctgggct tcaacgctct
     3541 acacggcaac attggtaaat gtagatccag ccttagtaga agcagctcga ctggatggag
     3601 ccaatatctt ccaacgaatc tggcacattg atattccagc tcttaagcct attatggtta
     3661 tccaatttgt tttagctgca ggtggaatta tgaatgtcgg atatgaaaaa gcattcttga
     3721 tgcagacatc gttaaatttg ccaacttctg aaattatctc gacatatgtc tataaagttg
     3781 gtcttgtatc aggagactat tcttactcaa cagcggttgg tttgtttaat gcagtgatta
     3841 acgtagtatt gcttgttgca gttaaccaaa tcgttaaacg catgaataat ggtgaaggaa
     3901 tttaaggagg aaagtatgaa aaattcgatt atggatacaa aatttgatag acgtatctta
     3961 ctcttaaata aaatcattat tgtctttatc gttttgatga ctttgcttcc tttactttat
     4021 atcgtcgtag catcctttat ggatcctaag gttctggtta gtagagggat tagctttaat
     4081 ccagccgatt ggactgtaga aggttaccag cgtgtattca gtgaccaatc tattctaaga
     4141 ggttttatca attctctact atactctttt ggatttgcag ctttaacagt attgctatct
     4201 gtgtttacag cttatcctct ttctaagaaa gacttggttg gacgtcgttg gattaactac
     4261 ttcttgattg tgactatgtt ctttggtggt ggtttagtcc caacttactt gctcgtaaaa
     4321 gaattgggaa tgctcaatac tccatgggct atcattgttc caggtgctgt caatgtttgg
     4381 aatattattc ttgctagggc ctatttccaa ggattgcctg aagaattagt tgaagctgct
     4441 gtcattgatg gagcgaatga cttgcagatt ttctttaaaa tcatgcttcc tcttgcaaaa
     4501 ccaattatgt ttgttctctt cctctatgct tttgtaggac agtggaactc atactttgat
     4561 gcaatgattt atatcaagga tccaaacttg gaaccattgc aacttgtact tcgtaaaatt
     4621 ctcattcaga gccaaccagg tcaagacatg attggagcac aagcggctat gaatgaaatg
     4681 aaacgtttag ctgaattgat taaatacgca actattgtca tttccagctt gccattgatt
     4741 gttatgtatc cattcttcca aaaatacttt gataaaggaa ttatggctgg ttcacttaaa
     4801 ggataaaaaa agaaaaaata aaaggagttt tctcatgaaa ttcaaaacat tctcaaaatc
     4861 agcagttttg ttgacagcta gtttagcagt acttgcagcc tgtggctcaa aaaatacagc
     4921 ttcaagtcca gattataagt tggaaggtgt aacattcccg cttcaagaaa agaaaacatt
     4981 gaagtttatg acagccagtt caccgttatc tcctaaagac ccaaatgaaa agttaatttt
     5041 gcaacgtttg gagaaggaaa ctggcgttca tattgactgg accaactacc aatccgactt
     5101 tgcagaaaaa cgtaacttgg atatttctag tggtgattta ccagatgcta tccacaacga
     5161 cggagcttca gatgtggact tgatgaactg ggctaaaaaa ggtgttatta ttccagttga
     5221 aaatttgatt gataaataca tgccaaatct taagaaaatt ttggatgaga aaccagagta
     5281 caaggccttg atgacagcac ctgatgggca catttactca ttcccatgga ttgaagagct
     5341 tggagatggt aaagagtcta ttcacagcgt caacgatatg gcttggatta acaaagattg
     5401 gcttaagaaa cttggtcttg aaatgccaaa aactactgat gatttgatta aagtcctaga
     5461 agctttcaaa aacggggatc caaatggaaa tggagaggct gatgaaattc cattttcatt
     5521 tattagtggt aacggaaacg aagattttaa attcctattt gctgcatttg gtatagggga
     5581 taacgatgat catttagtag taggaaatga tggcaaagtt gatttcacag cagataacga
     5641 taactataaa gaaggtgtca aatttatccg tcaattgcaa gaaaaaggct tgattgataa
     5701 agaagctttc gaacatgatt ggaatagtta cattgctaaa ggtcatgatc agaaatttgg
     5761 tgtttacttt acatgggata agaataatgt tactggaagt aacgaaagtt atgatgtttt
     5821 accagtactt gctggaccaa gtggtcaaaa acacgtagct cgtacaaacg gtatgggatt
     5881 tgcacgtgac aagatggtta ttaccagtgt aaacaaaaac ctagaattga cagctaaatg
     5941 gattgatgca caatacgctc cactccaatc tgtgcaaaat aactggggaa cttacggaga
     6001 tgacaaacaa caaaacattt ttgaattgga tcaagcgtca aatagtctaa aacacttacc
     6061 actaaacgga actgcaccag cagaacttcg tcaaaagact gaagtaggag gaccactagc
     6121 tattctagat tcatactatg gtaaagtaac aaccatgcct gatgatgcca aatggcgttt
     6181 ggatcttatc aaagaatatt atgttcctta catgagcaat gacaataact atccaagagt
     6241 ctttatgaca caggaagatt tggacaagat tgcccatatc gaagcagata tgaatgacta
     6301 tatctaccgt aaacgtgctg aatggattgt aaatggcaat attgatactg agtgggatga
     6361 ttacaagaaa gaacttgaaa aatacggact ttctgattac ctcgctatta aacaaaaata
     6421 ctacgatcaa taccaagcaa acaaaaacta gaggttgatt atgggagata agaaatacac
     6481 agtagaaaaa gccaatcgtt ttatagcaga aaataaacat ctcgttaata ctcaatataa
     6541 gcctgaagaa catttttcag ctgagattgg ttggatcaat gatccaaatg gatttgtcta
     6601 ttttcgtgga gaataccatc tcttttatca attctatcca tatgatagtg tttgggggcc
     6661 tatgcactgg ggacatgcta aaagtaagga cttggtgact tgggagcact tgccagtggc
     6721 acttgctcct gaccaagatt atgaccgaaa tggttgtttc tcaggctctg ccattgtcaa
     6781 ggatgatcgc ctctggctca tgtacactgg acatatcgaa gaagaaaccg gtgtccgcca
     6841 agtgcaaaat atggcatttt cagatgacgg gattcacttt gaaaagattg aacaaaatcc
     6901 agttgcaact ggatcagact tgccagatga gttgattgct gctgattttc gtgatccaaa
     6961 actctttgaa aaagatggac gctattactc cgtagtagct gccaaacaca agaataatgt
     7021 gggctgtatc gttctactag ggtccgataa cctagtagaa tggcagttcg aatccatctt
     7081 tttaaaaggg gtagaacacc aaggctttat gtgggaatgc ccagattact tcgagttaga
     7141 tgggaaagat tgccttatta tgtcacccat gcgttatcag cgtgagggag actcatatca
     7201 taacatcaac tcatcgcttt tgttcacggg taaggtagat tggggagaaa aatgttttat
     7261 cccagaatca gttcaagaaa ttgatcatgg ccaagacttc tatgcgcctc aaacattgtt
     7321 ggacgatcaa aatcgtcgta tcctgattgc ttggatgcag acatgggggc gtacccttcc
     7381 aacccatgac caagaacaca aatgggcatg tgccatgact ttacctagaa ttttaagatt
     7441 ggaagatggc aaactaagac aattccctgt taaaaaaggc caatatcaaa tccaaataga
     7501 taaagattgt cattaccact taggaaatga tatagattat cttgaatttg gttatgatag
     7561 taatgcgcag caagtttaca ttgatcgtag ccatcttatt caaaaaattc taggtgaaga
     7621 agaacaggac actagtcgac ggtatgtaga tattgaagct aaagaattgg aagttgttct
     7681 agataaaaat tccatcgaga tttttgtcaa tcaaggtgaa gcaagcttga ctgcaactta
     7741 ttacttaacg gtgccagctg agctatcacg aattgattaa aaattaagtt atttctccta
     7801 aagaaaaagt tctctttcta aaatagtgga aagaggactt tttgtgtttt gggtatataa
     7861 gcttagttta tggtatttgt aaaattggtg ttggattatg atttaaacta gttttctaaa
     7921 gaatttgaaa aaaattttat ttaagcaaaa aaagc
//
LOCUS       DAVKGC010000093         7935 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKGC010000093 DAVKGC010000000
VERSION     DAVKGC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.093"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(75..1241)
                     /locus_tag="VW049_001660"
     CDS             complement(75..1241)
                     /locus_tag="VW049_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9432741.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            1299..2459
                     /locus_tag="VW049_001661"
     CDS             1299..2459
                     /locus_tag="VW049_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9432742.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRIVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIIEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNMLQ"
     gene            2498..4348
                     /locus_tag="VW049_001662"
     CDS             2498..4348
                     /locus_tag="VW049_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9432743.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(4606..5238)
                     /locus_tag="VW049_001663"
     CDS             complement(4606..5238)
                     /locus_tag="VW049_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9432744.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGINEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(5260..6132)
                     /gene="sdaAA"
                     /locus_tag="VW049_001664"
     CDS             complement(5260..6132)
                     /gene="sdaAA"
                     /locus_tag="VW049_001664"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9432745.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(6141..6812)
                     /gene="sdaAB"
                     /locus_tag="VW049_001665"
     CDS             complement(6141..6812)
                     /gene="sdaAB"
                     /locus_tag="VW049_001665"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9432746.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            7054..7641
                     /locus_tag="VW049_001666"
     CDS             7054..7641
                     /locus_tag="VW049_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9432747.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(7693..>7935)
                     /locus_tag="VW049_001667"
     CDS             complement(7693..>7935)
                     /locus_tag="VW049_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9432748.1"
                     /translation="LYLYNQDKNMCKQICYTLLEDAKNKKSYDRLAICYVPIGICTDD
                     SKLIQKGFSLLELTEETSMLSHLKKEVEIYYQAKER"
BASE COUNT         2378 a         1461 c         1765 g         2331 t
ORIGIN      
        1 tctaaaagaa agccctgtca agagaaatgt gtaaaatttt tatatataaa aaacttctag
       61 ctaaaactag aagtttaaag gatcttatcc gctctgtcca ctgtaaagag ggccacagtc
      121 atcaggatat agatgagcaa gagggcagct acagctggta cccaagagtg gaacaggtca
      181 aaactgtaac caaagagggt tggtccaaag gctgctagga tatagcctcc tgtttgagat
      241 aggccggaca attgggctgt cttttcaggg gcgcttgtct tgagtgaaaa gttgaccatg
      301 agataaggga agagggcgct ggttgcggtt ccgatgagga gatggatggt aagccagtaa
      361 atgaaattat tgattgggaa aaagagcatg gaaatgccga ccacaccagc tagtgaaacc
      421 agagtgagca tgagctgacg gttgcgagta gataaactgg ttgtcaggct tgggatggtc
      481 attgaaaaag gaatgctaat cagagataag atagaagtca gcaagccagc ttcgtgactg
      541 gatagacctg catggataga catggtaggt aaccaggtca tgacggtgta aaagatcaag
      601 gattgaaaac ctgaaaagat aataattgcc caaacctgtt tattacgcat gacctttatt
      661 tgactttttt gtttggtttg tggagctagt ctatgattat agcggtgatt tgggagccag
      721 accaaaaaag ttgctagaca gagtaacgtg aggaggagga taagtccttt ccaagaactg
      781 gcttgtgtaa tgggcacagc tagataggaa gccagagccg ttgcaatccc catagacgtt
      841 acatataagg tggtcagaaa accaattttc tttggttgat tggcttggat aagactagga
      901 agcaggacat tgatgactgc gacacttgcc ccaaccatca aggttcctag atagagcagg
      961 ggcagattga ttagtcgaat gagtgagccg atggtcaaga agaagaggct gtaggtgaag
     1021 agatgctcca agccgatttt ctgagccagt cgggtagaaa atggtgaaaa gagggtaaac
     1081 ataaggagag gcaggctggt caagacacca agcgaactaa cttctacctc cagaccttgc
     1141 gaaatatttc ccaaaataat gggtaaaaca gtaaaaggag ttcgcaagga aacaccaatc
     1201 aggataatac ctggaacaaa aaagagtgat tgctttttca tataatacct cttctagctg
     1261 attttaataa cagcttattt taaggctttt tcactataat gtcaagtaag gtttctggct
     1321 ttcaagataa aaatgggaaa gtcttgaaaa ttatgataga atggtggaag gaaaaattca
     1381 ggagaatagt agtgactcaa aatgttgaaa gtcttctcgt atccattgta atcagtgcat
     1441 acaatgaaga aaaatatctg cctggtctaa ttgaagactt aaaaaatcaa acttatccta
     1501 aagagaatat tgaaattcta tttataaatg ctatgtccac agatgggacc acagctatca
     1561 ttcagcaatt tataaaggaa gatacagagt ttaactcaat tagattgtat aacaatccta
     1621 agaaaaatca agctagtggt tttaacctgg gagttaaaca ttctgtaggg gaccttattt
     1681 taaaaattga tgctcattca aaagttactg agagttttgt aatgaacaat gtggctatta
     1741 ttcaacaagg tgaatttgtc tgtggggggc ctagaccgac gattatcgaa ggaaaaggaa
     1801 aatgggcaga gaccttgcat cttgttgagg aaaatatgtt tggcagtagc attgccaatt
     1861 atcgaaatag ttccgaggat agatatgttt cttctatttt tcatgggatg tataaacgag
     1921 aggttttcca gaaggttggt ttagtaaatg agcaacttgg ccgaactgaa gataatgata
     1981 ttcattatag aattcgagaa catggttata aaatccgcta tagcccaagt attctatctt
     2041 atcagtatat tcgaccaaca ttcaagaaaa tgctgcatca aaagtattca aatggtttgt
     2101 ggattggctt gacaagtcat gttcagttta agtgtttatc attatttcac tatgttcctt
     2161 gtttatttgt tttgagtctt gtgtttagtc tagcattgtt accgatcaca ttcgtattca
     2221 taactttact attaggtgcc tattttctac ttttgtcact actcactttg ctgactttat
     2281 taaaacataa aaatggattt ctaattgtga tgccctttct tttattttcc attcactttg
     2341 cttatggctt tgggacgatt gtaggtttaa ttagaggatt taaatggaag aaggagtaca
     2401 agggaacaat aatttatttg gataaaataa gccaaataaa tcaaaatatg ctacaataac
     2461 aatatagtaa aactctttta aggaggagta gatttctatg aataaaaaac taacagatta
     2521 tgtgattgat ctggtggaaa ttttaaataa acaacaaaag caggttttct ggggaatatt
     2581 tgatattttc agtatggtgg tttccatcat tgtatcttat attttatttt atgggctgat
     2641 taatccagca cctgttgact acattatcta tacgagtttg gccttcttgt tctatcaatt
     2701 gatgattggt ttttgggggt tgaacgcgag cattagtcgt tacagcaaga ttacggattt
     2761 catgaaaatc ttttttggtg tgactgctag cagtgtcttg tcatatagta tctgttatgc
     2821 cttcttgcca ctcttctcca tccgtttcat cattctcttt atcttgttga gtaccttctt
     2881 gattttattg ccacggatta cttggcagtt aatctactcc agacgcaaaa aaggtagtgg
     2941 tgatggagaa caccgtcgga ccttcttgat tggtgccggt gatggtgggg ctctctttat
     3001 ggatagttac caacatccaa ccagtgaatt agaactggtc ggtattttgg ataaggattc
     3061 taagaaaaag ggtcaaaaac ttggtggtat tcctgttttg ggctcttatg acaatctgcc
     3121 tgaattagcc aaacgccatc aaatcgagcg tgtcatcgtt gcgattccgt cgctggatcc
     3181 gtcagaatat gagcgtatct tgcagatgtg taataagctg ggtgtcaaat gttacaagat
     3241 gcctaaggtt gaaactgttg ttcagggcct tcaccaagca ggtactggct tccaaaaaat
     3301 tgatattacg gaccttttgg gtcgtcagga aatccgtctt gacgaatcgc gtctgggtgc
     3361 agaactgaca ggtaagacca tcttagtcac aggagctgga ggttcaatcg gttctgaaat
     3421 ctgtcgtcaa gttagtcgct tcaatcctga acgcattgtc ttgctcggtc atggggaaaa
     3481 ctcaatctac cttgtttatc atgaattgat tcgtaagttc caagggattg attatgtacc
     3541 tgtgattgcg gacattcaag actatgatcg tttgttgcaa gtctttgagc agtacaaacc
     3601 tgctattgtt tatcatgcgg ctgcccacaa gcatgttcct atgatggagc gcaatccaaa
     3661 agaagccttc aaaaacaata tccgtggaac ttacaatgtt gctaaggctg ttgatgaagc
     3721 taaagtgtct aagatggtta tgatttcgac agataaggca gtcaatccac caaatgttat
     3781 gggagcaacc aagcgcgtgg cggagttgat tgtcactggc tttaaccaac gtagccaatc
     3841 aacctactgt gcagttcgtt ttgggaatgt tcttggtagc cgtggtagtg tcattccagt
     3901 ctttgaacgt caaattgctg aaggtgggcc tgtaacggtg acagacttcc gcatgacccg
     3961 ttactttatg accattccag aagctagccg tctggttatc catgctggtg cttatgccaa
     4021 agatggggaa gtctttatcc ttgatatggg taaaccagtc aagatttatg acttggccaa
     4081 gaagatggtg cttctaagtg gccacactga aagtgaaatt ccaatcgttg aagttggaat
     4141 ccgcccaggt gaaaaactct acgaagaact cttggtatca accgaactcg ttgacaatca
     4201 agttatggat aagattttcg ttggtaaggt taatgtcatg cctttagaat ccatcaatca
     4261 aaagattgga gagttccgca ctctcagtgg agatgagttg aagcaagcta ttatcgcctt
     4321 tgctaatcaa acaacccaca ttgaataaaa aagaaaaacg catattatca agttaaacaa
     4381 ccttggtaat atgcgtttta ttatgtagag acttatactc ttcgaaaatc tcttcaaatc
     4441 acgtcaacgt cgccttgccg tatatggtta ctgacttcgt cagttctatc cacaacctta
     4501 aaacagtgtt tagagcagcc cgcagctagt ttactagttt gctctttgat tttcattgag
     4561 tattacttca ttttcttctg aaatagagtt gtcgcccagt ctatgctatt gaaaatacgc
     4621 caaaacttct aagggtttgt gagcgatata atcaggttga tagtttagta gatctgcttg
     4681 ctctccaaat ccccaagtga cggccaattt ctgaatacct gtttctcgag ctcccagcat
     4741 atcaaacttg gtatctccga tgatgatggc ttgttctggt gctagttgat gtgtctgtaa
     4801 ggcttggtga atgacatctg ccttatgggg tgcttcaggg ctagaaccat aaataccatc
     4861 aaagaaatga tggatttcca agtttttggc catgtcttga gcagtagatg tattctttgt
     4921 cgtggtgatg tagagtggat aactgctcga taactcctca agcaagtcta taatctgagg
     4981 aaagagttga gcttcattga tgccttttgc cttatagtaa gaacgatata tctgcatggc
     5041 ttcagaaatt tggtctttgg acaggcaggt cgcaaaacta ctttcgagag gtggtcccat
     5101 aaaaccacga atagttttgg catcagggct aggcaccccc agctctttaa aggtataggt
     5161 aaaggcattg tgaatcccga tagaactatc aacgagggtt ccatccaaat cgaaaaaaat
     5221 cgctgtgata gaggtcatgg tttctcctat ttgataagct tattctccga aaatctcttt
     5281 ttggaggcga cgaccagtag gggtggtagc gagtccacct tcagctgttt cacgaaaggc
     5341 agttggcatg cttgctccta cttggtacat ggcatcgatc acttcatcca cagggatttt
     5401 agattcgata cctgccaagg ccatgtctgc tgcgatgaaa gcaaagctag ctcccatggc
     5461 attacgtttg acacagggaa cttcgaccaa acctgcaaca gggtcacaga tgaggcctag
     5521 catattttta atgacaaagg caatagcttg actggcctga taaggtgttc cacctgcagc
     5581 cagagtcaag gcggcagcac tcatagcaga ggctgaacca acttcagctt gacacccacc
     5641 ctcagcacct gagatggagg cattgtttgc gatgactagt ccaaaggcac cagcagcaaa
     5701 gaggaaatcc aattgttgct cgtggctgag gtctaatttt tcaatagcag cagtgagaac
     5761 ggaagggaga caaccggcac ttcctgcggt aggagtagcg cagaccaagc ccattttagc
     5821 attgtgttca ttgactgcga tagcatttcg ggcagcagag agaatcgtgt aatctgataa
     5881 agttttttcg tttttgatgt agtggtccaa tttggcagca tctccacctg tcaggccact
     5941 acgagattta ttttcattga ggccaagttg gacagaggct ttcataactt ccagattgcg
     6001 ttccatgaga aggaagactt cttcacgttc gcgaccggtc aattcaaact ctgttgtaat
     6061 catgagttct gcgacatttc cttgaaagtc cagatctgct tgctcgacca attctttgat
     6121 agaataaaac atgcttcctc ctatttaaag aaattgacat tgtggagatg agggattttt
     6181 cgaatttctt cgatagcctc atcacagttg cgactgtcaa cttcgataat cataatggct
     6241 ttttcaccag ctttttcacg agtgacattc atctgggcga tattgatacc atagcgggaa
     6301 agcgcctctg taacaagggc aatcatacct ggaatatctt gatgaacgat gatgatagtc
     6361 ggtgtattca tattgagaga gacggcaaaa ccattgagtt cggttacttg aatatttcct
     6421 ccaccgatag aaataccagt cacgctgatg gtcttgtggg catttttaac agtaatttta
     6481 gtggtgttag ggtgaggggc attgctgtct ttctgaatgg tccagacaat cttgatacca
     6541 cgcttgtggg caatttccag actatttgga atttcaggat catctgtatc cattcctaaa
     6601 atacctgcaa caagggctag gtctgttccg tgaccacgat aggtcttggc aaatgagtta
     6661 aaaagttgga attcaacttc tgtcggagta tcatcaaaaa tggaagagac aatcttccca
     6721 atacgaacag caccagcggt atggctacta gatgggccaa tcataactgg tccgatgata
     6781 tcaaagacag attgaaaacg aagtgatttc atcagtttcc ccttataaaa attcttatct
     6841 ctattatatc aaagaatgag gggcttggct ttaattgtgg atgaaaacct ttctaatacc
     6901 tcaaatagca taaaaatagt atcttttatg acaaaaaaca ccttatttag ggaaataaaa
     6961 aataattttg taatatttct acataaaagt gtcaagaaac ggtaatattt aaagggtatg
     7021 atagaactat agaaagaagg agaattttcg aatatgaaat caataactaa aaagattaaa
     7081 gcaactcttg caggagtagc tgccttgttt gcagtatttg ctccatcatt tgtatctgct
     7141 caagaatcat caacttacac tgttaaagaa ggtgatacac tttcagaaat cgctgaaact
     7201 cacaacacaa cagttgaaaa attggcagaa aacaaccaca ttgataacat tcatttgatt
     7261 tatgttgatc aagagttggt tatcgatggc cctgtagcgc ctgttgcaac accagcgcca
     7321 gctacttatg cggcaccagc cgctcaagat gaaactgttt cagctccagt agcagaaact
     7381 ccagtagtaa gtgaaacagt tgtttcaact gtaagcggat ctgaagcaga agccaaagaa
     7441 tggatcgctc aaaaagaatc aggtggtagc tatacagcta caaatggacg ttatatcgga
     7501 cgttaccaat taacagattc atacctgaac ggtgactact cagctgaaaa ccaagaacgt
     7561 gtagcagatg cctacgttgc aggacgttac ggttcatgga ctgctgctaa aaacttctgg
     7621 cttaacaatg gctggtatta agaactaacg aacaaataat atgtaaagag tcgaggaaat
     7681 ccctcggctt ttttatcttt ccttcgcttg gtaatagatc tctacttctt ttttgagatg
     7741 agacagcata gaagtttcct cggttagctc cagaagggag aacccttttt ggataagttt
     7801 agaatcgtct gtacaaatcc caatagggac atagcagata gccagcctat cgtagctttt
     7861 cttattcttg gcgtcctcta gtaaagtgta gcagatttgc ttacacatgt ttttatcttg
     7921 attgtataga tagag
//
LOCUS       DAVKGC010000094         7840 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKGC010000094 DAVKGC010000000
VERSION     DAVKGC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.094"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(556..1404)
                     /locus_tag="VW049_001668"
     CDS             complement(556..1404)
                     /locus_tag="VW049_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9432749.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTESHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1556..1834
                     /locus_tag="VW049_001669"
     CDS             1556..1834
                     /locus_tag="VW049_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432750.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1836..3899
                     /locus_tag="VW049_001670"
     CDS             1836..3899
                     /locus_tag="VW049_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9432751.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREIVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(3977..5233)
                     /locus_tag="VW049_001671"
     CDS             complement(3977..5233)
                     /locus_tag="VW049_001671"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9432752.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5375..7840
                     /locus_tag="VW049_001672"
     CDS             5375..7840
                     /locus_tag="VW049_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9432753.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
BASE COUNT         2323 a         1343 c         1763 g         2411 t
ORIGIN      
        1 atatgctatt atgttaaaaa aatattttta aaaatataaa tggacggatt tattttggat
       61 tttatttgtt attttgacct gcctctatat tggtaaccat gatttgttta ctttcaatca
      121 tcaagaattc tcttttcgtg gtagcgtttg gggtctggta ctggccttat atcacttatt
      181 gttcattgat aagtttgtaa tttcgaatcg aaaataaaga ttagaactat gattgactgg
      241 ctacttttaa tattgatttt ggaggaagat tttgtctcta ttatttatta ttttaaattt
      301 atctattttg tacaagatct attctttgag acgggagaaa tcgaaatact tgatttatac
      361 ggcctatatc atatttgggg taaatgtagt atatggtatt caatggttat taagagaact
      421 gatttcaact atttcccctt aatgtgtatg gaaactataa caaaacgcat aagattaagg
      481 tttgtgtacc tgttcttatg cgtttttgta atattgaaga ttttcggtaa gttttatacc
      541 tttgggaagt ctagtttaga atgctttccc aaccaaaatc tctgcttcga tggtaatctc
      601 tgtcagttgg ctccagtcaa tcttgctctg gtcaacgtga aagaggagag gagtcatact
      661 gagtagggct tggagttgtt ctgctgtgat agtcttagtc agagaggcag tttgactaga
      721 taggatggta aagtgttcct ggaaatgctc cttgatatct tggttagaat actccttgtt
      781 tgtcagctgg tcctgtactc tttgacggat ttctttgagg tgactttcag ttgggataac
      841 ctttatcaag ataccgtctt tggataaaac gcgacgaaat tctccatagt tggcaggtga
      901 gaagatatca agcagaatat ccatgttagc gtcttttata ggaagtcgtg ccaagtcgcc
      961 aacgaaccaa ttgactgccc agttgggttc acttttagca gcgatttgga ctgaatcttt
     1021 ggagatgtca aaggcataga aagttttttc agagtgactt tcttgtagtt tgcgagaata
     1081 gaatccttca ccacaaccga tatccaaaat tgttgtggta gtttttgagc ttgcaagcaa
     1141 gtcagataca gcatctaaga tagcttggta aaagccggct tctaggattt gttgacggtt
     1201 ttgaaaattt tctttgtcat agttagcaga ttgcttgatt tgaggtgcta gattgacata
     1261 gccaaatttc gccaagtcaa aagaatgacg gttgcagcac ttgaaattag tctctaacag
     1321 agtcagattt tcttgacaga taggacaggc aaaggcagtc gcagaagcaa aacgctgaag
     1381 tttgggcttg agatttgtat tcatagttta agtgtatcaa aagaggcaaa tgaaagcaac
     1441 tttaggaata agtagatggg agattcagta gaaataaaac taattctcca atttatagaa
     1501 tgaaattgct tttatatcta aattgctata taataatgat aaggaggaaa taagtatgtt
     1561 aaaagaagta ttaaccgttg caaaagttgc gaaaaaatct tcattatttt tgggtggtgt
     1621 cgcatttggt acccttggtt tgaaaatctt agcaagtaag gaagctaaaa aaggttattc
     1681 taaagctttg gctaaggctt acaagttgaa agacgagcta gatgcatctg tttctgttgt
     1741 gaagcaacat ggagacgatg tcttgcaaga tgccaaatat ttgtacgagc aagagaaaaa
     1801 agaagagcaa ttagatagcc ttataggtga ataatatgtc ttttaaagtg ctacatagag
     1861 gataccaaca tatccgacta tcatcttctt tttcacttac cttggatatt caagactatc
     1921 ttcgttcctt ggcgagagat gaaaagggga ttgagtctat ccagttttac atggatcaac
     1981 agcactttac tctacgcata aaagaaggct tttctgtatt agataatgca gaagcctttt
     2041 taaaaagaat tgataaaggg aaagtttctg agttgatgac tcttcccatt cgtagagaag
     2101 agagtgctta ttctattgtt tcaggtgcag cggttaagcg tgtacttttt cgtagttttg
     2161 tgccgtatcc tattcgctat atatggactt gttatcaggc tttgggttat attagagaag
     2221 cctatcaaac actagcgcgt aaggaactaa cgatggaagt cttggactgt tcggctattt
     2281 tattgtcctt gtttatgaac caatccaaga cagctagcaa tatcatgttt atgcttgatt
     2341 tggggaatca tttagatcag tggtccttga aaaaaactgc aacagattta gaacagagtc
     2401 ttcttgcaaa agagagcgat gtattcctag tacagggcga tacggttgtt agtataaaga
     2461 gttccgatgt tcaaatagga gatgtcttga tcttatctca aggaaatgaa attctgtttg
     2521 atgggcaagt agtttcaggt ttaggtatgg tcaacgaaag ttccttgaca ggagagagtt
     2581 ttccagttga aaaaagagag tctgatttgg tttgtgcaaa tacagtatta gaaactggag
     2641 agttacgcat tcgtgtaaca gataatcaga tgaacagccg tattttacag ctgattgagt
     2701 tgatgaagaa atctgaagaa aacaagaaaa cgaaacaacg ctatttcatc aagatggcgg
     2761 ataaagtcgt caaatataat ttcttggggg ctgggctgac ttacctattg acaggttctt
     2821 tttctaaggc tatttctttc ctattggtcg atttctcctg cgctttgaaa atctctactc
     2881 ctgtagctta tttgacagtt atcaaggaag ggttgaaccg tgaaatagtg attaaggatg
     2941 gagatgttct ggagaaatat ctggaagttg atactttctt gtttgataag acaggaacaa
     3001 tcacaactag ttatcctata gttgaaaagg tgttaccttt tggagactat agtgaggaag
     3061 atattctcag aatcagtgcc tgtcttgagg aacacattta tcatcctatt gctaatgcca
     3121 tcgtcaagca agctgagata gagggaattg aacatgagga aatgcatggg aaactccaat
     3181 atatcgcaag caaggggatc aaatctcata tagatggcca accagttctt attgggaatt
     3241 atgtcttgat gcaggatgag cagattcata tcagttcaga acaaaatgct ttaattgaag
     3301 agtacaagag tcactacaat ctcttattct tggcttatca aaatgaattg attggaatgt
     3361 tctgcattca tactcctttg agaaaagaag caaaaacagc cttggataaa cttaaggcac
     3421 aggggaaaaa attaattctg gcaacaggag acaccttgat taggacagag gaattagtca
     3481 aagatttgcc ctttgatcag gtctatacag acttgaaacc tgatgggaaa tttgagttag
     3541 tagagaaact gcagaaagca ggtcacacta ttttgatggt tggagatgga ttgaatgact
     3601 cagcggcttt aactctatca gatatcggtg tggtgatgaa tgagagtgca gatatttcta
     3661 agcagatgag tgatatctta ttgttagata atcgtttgga tttcttccaa gagttggatt
     3721 cgttatcatc atctttgcaa acactcatca agaaaaatat tcaagatacc gttgtcgtaa
     3781 atagtagttt gattggcttt ggcttattta attggttcag tccttcaaat ctctctatct
     3841 tacataatct aacaaccttg cgcattgtct tgcgtagctt gtctattaaa aatagataga
     3901 tgagagttat taacagcaaa aaggagttac ctttctcgag gttaactcct ttgtttatag
     3961 ataaatgttg aatagtttag taagtcaata caaagtattt tttcttccca cgacggataa
     4021 cagtcagttc attttctaac ttatcagcgt cactcaagac atagtcaagg tcttggatgc
     4081 ggtcgccgtt tacgtagatg gctccgtttt ggacgtcttc acgggcttgg cgttttgagt
     4141 taactatacc agatgagacg agcagttcca cgatattgtt atgttcgtct gcctgtactt
     4201 ggtagtttgg cacaccacga agtccttgtt tgagctcttt gacagaaagg tttttgatgt
     4261 ttcctgcaaa gagttgctca gtgatgttaa gtgcttcttt gtaggcttct tctccgtgaa
     4321 caagtgtaac aacttcacga gccaagactt tttgagccaa gcgttcgtgt ggcgctgctt
     4381 caaattgttt acgaatatct tcaatctcat caagtgacaa gaaagtaaag attttcaaga
     4441 agcgaacagc gtcagcgtcc atcacgttca tccagaattg gtacatttcg tatggagaag
     4501 tcttttcggg attgagccat acggcatttc cttctgattt accaaatttc ttaccagttg
     4561 catctgtgat tagtggaaca gtgataacgt gaccagtctt gtccgcctta cgacgaagca
     4621 attcggtacc agctgtcata tttccccact ggtcagaacc accgatttga agagtgacat
     4681 tatggtcttg gttaaggacg aagaagtcgt acccttgcat gatttggtaa gcgaactcag
     4741 tgtaagaaat tcctgtttcg atccgttttt taacagattc cttactcatc atgtagttga
     4801 ccgtgaagta ttttccaata tcacggagga agtcaatgaa gctgatgctg ccaaaccagt
     4861 cgtagttgtt gaccatgaca gccttgtttt cgccattttc aaagtcaaga aaacgagaaa
     4921 gttgtccttg gatagacttg acccagccat ctactgtgtc ttttgtttgg agactacgtt
     4981 cagcatcttt gaaggacgga tctccgatga gacctgtagc accgccaacg agcgcataag
     5041 gtttgtgacc tgctagttgc aagcgacgac ttgtcaagat tgcgacaagg tggcctaggt
     5101 gaaggctgtc agcagttgga tcgtagccag tataataaga aacttgacct tcttctaggg
     5161 ctttacgcaa agcttcttca tcagtcgttt gaaatatcaa accacgctct tttagctcat
     5221 caaaaatgtg catgtgtctt ttctcctttt taaaattatt gtttctacct attgtatcac
     5281 aaacttgggc aatagcctag cagaataggg aagaaagtgg ctattttatt gaaaatttcc
     5341 cttttgtggt ataatagata gagtgaggaa atccatgcaa aatcaattaa atgaattaaa
     5401 acgaaaaatg ctggaatttt tccagcaaaa acaaaaaaat aaaaaatcag ctagacctgg
     5461 caagaaaggt tcaagtacca aaaaatctaa aaccttagat aagtcagcca ttttcccagc
     5521 tattttactg agtataaaag ccttatttaa cttactcttt gtactcggtt ttctaggagg
     5581 aatgttggga gttgggattg ctttggggta cggagtggcc ttatttgaca aggttcgggt
     5641 gcctcagaca gaagaattgg tgaatcaggt caaggacatc tcttctattt cagagattac
     5701 ctattcggac gggacggtga ttgcttccat agagagtgat ttgttgcgca cttctatctc
     5761 atctgagcaa atttcggaaa atctgaagaa ggctatcatt gcgacagaag atgaacactt
     5821 taaagaacat aagggtgtag tacccaaggc ggtgattcgt gcgaccttgg ggaaatttgt
     5881 aggtttgggt tcctctagtg ggggttcaac cttgacccag caactaatta aacagcaggt
     5941 ggttggggat gcgccgacct tggctcgtaa ggcggcagag attgtggatg ctcttgcctt
     6001 ggaacgcgcc atgaataaag atgagatttt aacgacctat ctcaatgtgg ctccctttgg
     6061 ccgaaataat aagggacaga atattgcagg ggctcggcaa gcagctgagg gaattttcgg
     6121 tgtagatgcc agtcagttga ctgttcctca agcagcattt ttagcaggac ttccacagag
     6181 tcccattact tactctcctt atgaaaatac tggggagttg aagagtgatg aagacctaga
     6241 aattggctta agacgggcta aggcagttct ttacagtatg tatcgtacag gtgcattaag
     6301 caaagacgag tattctcagt acaaggatta tgaccttaaa caggactttt taccatcggg
     6361 cacggttaca ggaatttcac gagactattt atactttaca actttggcag aagctcaaga
     6421 acgtatgtat gactatctag ctcagagaga caatgtctcc gctaaggagt tgaaaaatga
     6481 ggcaactcag aagttttatc gagatttggc agccaaggaa attgaaaatg gtggttataa
     6541 gattactact accatagatc agaaaattca ttctgccatg caaagtgcgg ttgctgatta
     6601 tggctatctt ttagacgatg gaacaggtcg tgtagaagta gggaatgtct tgatggataa
     6661 ccaaacaggt gctattctag gctttgtagg tggtcgtaat tatcaagaaa atcaaaataa
     6721 tcatgccttt gataccaaac gttcgccagc ttctactacc aagcccttgc tggcctacgg
     6781 tattgctatt gaccagggct tgatgggaag tgaaacgatt ctatctaact atccaacaaa
     6841 ctttgctaat ggcaatccga ttatgtatgc taatagcaag ggaacaggaa tgatgacctt
     6901 gggagaagct ctgaactatt catggaatat ccctgcttac tggacctatc gtatgctccg
     6961 tgaaaagggt gttgatgtca agggttatat ggaaaagatg ggttacgaga ttcctgagta
     7021 cggtattgag agcttgccaa tgggtggtgg tattgaagtc acagttgccc agcataccaa
     7081 tggctatcag accttagcta ataatggagt ttatcatcag aagcatgtga tttcaaagat
     7141 tgaagcagca gatggtagag tggtgtatga gtatcaggat aaaccggttc aagtctattc
     7201 aaaagctact gcgacgatta tgcagggatt gctacgagaa gttctatcct ctcgtgtgac
     7261 aacaaccttc aagtctaacc tgacttcttt aaatcctact ctggctaatg cagattggat
     7321 tgggaagact ggtacaacca accaagacga aaatatgtgg ctcatgcttt cgacacctag
     7381 attaacccta ggtggctgga ttgggcatga tgataatcat tcattgtcac gtagagcagg
     7441 ttattctaat aactctaatt acatggctca tctggtaaat gcgattcagc aagcttcccc
     7501 aagcatttgg gggaacgagc gctttgcttt agatcctagt gtagtgaaat cggaagtctt
     7561 gaaatcaaca ggtcaaaaac cagggaaggt ttctgttgaa ggaaaagaag tagaggtcac
     7621 aggttcgact gttaccagct attgggctaa taagtcagga gcgccagcga caagttatcg
     7681 ctttgctatt ggcggaagtg atgcggatta tcagaatgct tggtctagta ttgtggggag
     7741 tctaccaact ccatccagct ccagcagttc aagtagtagt tctagcgata gcagtaactc
     7801 aagtactaca cgaccttctt cttcaagggc gagacgataa
//
LOCUS       DAVKGC010000095         7471 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKGC010000095 DAVKGC010000000
VERSION     DAVKGC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.095"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(59..1762)
                     /gene="ilvD"
                     /locus_tag="VW049_001673"
     CDS             complement(59..1762)
                     /gene="ilvD"
                     /locus_tag="VW049_001673"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9432754.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(1995..2927)
                     /locus_tag="VW049_001674"
     CDS             complement(1995..2927)
                     /locus_tag="VW049_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9432755.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(2924..3781)
                     /locus_tag="VW049_001675"
     CDS             complement(2924..3781)
                     /locus_tag="VW049_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9432756.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(3785..5131)
                     /locus_tag="VW049_001676"
     CDS             complement(3785..5131)
                     /locus_tag="VW049_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9432757.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(5144..5428)
                     /locus_tag="VW049_001677"
     CDS             complement(5144..5428)
                     /locus_tag="VW049_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9432758.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(5432..7462)
                     /locus_tag="VW049_001678"
     CDS             complement(5432..7462)
                     /locus_tag="VW049_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9432759.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEYYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
BASE COUNT         2403 a         1643 c         1157 g         2268 t
ORIGIN      
        1 aaccatacct acggcaaggc gacgttgacg tgatttgaag agattttcga gtatgagttt
       61 attttttacc tgacttgtcc atattccaga agtctgtcac ggctccgcgt gaagcagatg
      121 atacgatgtg ggcatattta ccgaggacac cacggctgta aagtggtggc aaggttgttt
      181 ctgccttgcg tttttcaagt tcttcttcgg atacggccat ggaaatttct ttggtatctt
      241 ggtcaaccgt aacgatatcg ccggtacgga gataggcaat tggtccacca tcctgagctt
      301 caggagcgat atgtccaaca accagaccat aagtaccacc agagaaacgt ccgtccgtca
      361 agagggccac cttatctccc tgacctttac caacaatcat tgaagaaagt gacagcatct
      421 caggcatacc aggaccacct ttaggtccaa caaaacgaac aacgactaca tcgccatcaa
      481 cgatttcatc tgtcagaacg gcctgaatcg catcttcttc tgagtcaaag accttagctg
      541 gcccaacgtg acgacgcact ttaacacctg ataccttggc aactgcaccg tcaggagcaa
      601 ggttcccgtt caagatgata agcggaccat ccgcacgttt tggattttca agtggcatga
      661 taactttttg gcctggagtc aagtctgcaa agtcagccaa gttttcagct acagtcttac
      721 cagtacatgt gatgcgatct ccgtgaagga aaccatttgc caacaagtac ttcataaccg
      781 cagggacacc accgacttcg tagaggtctt ggaagacata ctgaccagat ggtttcaagt
      841 cggccaagtg aggcacacgt tcttgaatcg tattgaagtc ctcaagtgac aagtcaacat
      901 ttgcggcatg ggcaatggcg agcaagtgaa gagtggcgtt tgtagaacca ccgagagcca
      961 tcgttacagt aatagcatct tcaaaggctt cacgagtcaa gatatctgat ggtttgagac
     1021 caagttccaa catcttaaca acagcacgtc ctgctgcttc gatatcttct ttcttatcag
     1081 ctgattcagc tgggtgagag gatgaccctg gcaaactcat ccctagaact tcgatagcag
     1141 ttgccatggt attagcagta tacataccac cacagccacc agggccaggg caggcattac
     1201 attcaagacg tttcacgtcc tcagctgtca tgtcaccgtg gttccatttt ccgatacctt
     1261 caaagacaga aaccaagtcg atatctttac catcaagatt tcccggtgca atagttccac
     1321 cataggcgaa aatagctggg atatccatat tagcaatagc aatcatagat ccaggcatgt
     1381 tcttgtcaca gccaccgata gcgacaaagg catccacgtt gtgaccactc atagccgcct
     1441 cgatggagtc cgcgatgatg tcacgagatg ttagagagaa acgcatacca ggcgttccca
     1501 tagcgatccc gtctgctacg gtaatggttc caaactgtac aggccaagcg cctgcagatt
     1561 tgacaccttc tttagccagt ttcccgaaat catgcaagtg aatgttacat ggtgtatttt
     1621 ccgcccaagt cgaaatcact cccacaatcg gtgtttcaaa gtccttatct gtcataccag
     1681 tcgcacgaag catagcacgg ttaggtgatt taaccatgct gtcataaatg ctactgcggt
     1741 gacgtttatc taattcagtc atcttatccc tcccatttca gtttttacta ttatagcaca
     1801 attttcgcat gaagaacaga ataaaattct tgaattttca gaaaattcta tacacatgtg
     1861 aaatatttaa aattaaaaac aacaaagcgg attagtgcac tttctgatga ccagaatatg
     1921 ctttttaatc cgctttcttt aaataacgta ctgtaatttt tacagaaatt ctttcaaata
     1981 agtgtattta acatctatct tgcattataa atttctagaa ccttctcttt tatattcgat
     2041 tcactcaaac catactcatt aagaagataa tccattttcc ctacttgacc gaatctttct
     2101 tgaacaccca tccgatgaat ttttgttatt ccatcatcag agaataattc acataaagca
     2161 ctgccaattc cacctatctg attgtggttt tctacagtaa atatagtttt tccacttaac
     2221 attgttttta tctgttctgg tatcggtttg attctaaata aatctatcac acctactgaa
     2281 taacctaatt tagacagttc atctgcaact cgaatacttg gagcaaccat tataccagaa
     2341 gcaacgatta caagatcttc cccatgtctt aactcaatgt agcctttaga aaaatcttct
     2401 ccaccttgat acacaggaac tggagctttt ctaattgttc gaatatattt tagtcctttt
     2461 aagtctaatg tctggttcaa tatttcacga aattggatat catcagttgc ttcaaaaatg
     2521 atcgatttag gaattaaacg taacaatcca atttcttcaa atggcatatg tgtcccacca
     2581 ttcatttctg cagttactcc tgcatctgat ccaatcacag tggcatccaa ttgtgcgtat
     2641 ccaagagaaa taaataattg atcaaatacc cttcgtgaag aaaaaggacc aaatgtatga
     2701 agataaggtc taaacccctg aatagacaag cctgctgcaa ggccgaccat ttctgcttcc
     2761 ataatcccaa cattcacata acggtctcca aagtcctttt caagattatg agtagccatc
     2821 gaacttgaca gatcggcttc taagactact atatcagaat aactttgatt agcctctaaa
     2881 aggaagtctc tatatacatg ccgtaattct ttcgtacttc tcatcattct gtttcctcca
     2941 attcctgact taatctttct acaactgaag ttaacatttg tctctcctct acagtagggc
     3001 gaagatgatg attggatttc atttcttcca gctcttgaac cccttgacct ttaatagtat
     3061 ctaatacaat gcacttaggt gatgaattat ttgactgttt taattggaca atcccttcat
     3121 aaatttctct aatatctgaa cccttgaccc taatggattc aaatccaaat gctgaaaatt
     3181 tttctacgaa atcacctgga ttacaaatat cctttgtaaa accatctaat tgttttttgt
     3241 tatcatcaac aaatacaatt aagttggata actgttgatg agaagcaaac tgtatagcct
     3301 cccaacattg tccctcattt aactcaccat ctccaacaat agcataagta taaaagggac
     3361 tctttcttat tctctgacca tatgcaagtc cagttgcaac actaattcct tgtcctaaag
     3421 agcccgttgt catatctatg cctggcgtta gatttctatc aggatgagac ggtaatttgg
     3481 ttccatttgt atttaaagaa tataagaatt ctttgtcaaa gaaaccattc aaatagagtg
     3541 tactgtatag agctggtcct ccatgacctt ttgataatat gaaataatct ctatctcgtg
     3601 ctgcaaatat ttctggagtc attggcatta tttcaccata aagcactgct aaaacttcta
     3661 cgatagacag acttcctccg taatgtccga atccaagatg attcaatgtt ctaagagtat
     3721 ttaatcggat gttagtcgca aattttctta acccatcttc tctattttta cttaaaatca
     3781 tcccttattc ctcctttgca gatggctttt taataaagga tactccaaac ataactgcta
     3841 gaataagaac aagaccaatc acaatgcctg cttgtgagcc aaattgattt aacattccta
     3901 aaataattcc tgatagacca aaatctgcat ctgagaaagt tgatccttgg aaaccaagtc
     3961 ctcccaaaac tggcattaaa aagactggaa gaaaactgat taaaatacct tgtaaaaatg
     4021 ctccaatagt ggctccacga acaccaccag atgcattccc aatgacacct gcagtcgctc
     4081 cacagaagaa atgaggcaca acacctggta agataacaac cgttcctgaa gcaatcataa
     4141 ttgccatact tactaaacca ccaataaaac tagagataaa tccaattaga actgcattgg
     4201 gtgcataagt ataaacaatc ggacaatcca aagcaggttt tgaattaggt acaagacgct
     4261 ctgaaatacc tttaaaggct ggaacaattt cgcccaaaat aaggcgaaca cctgctaaaa
     4321 taacaaatac ccctgctgca aattgacctg ctaattgtaa agcataaact agaccacttg
     4381 taccactact gatttctttt tctatatatt ctgaccctgc aaagatagct acaataatgt
     4441 aaataactgc catggataaa gtaatactaa cagtactatc acgtaaaaaa gctaaactct
     4501 ttggaaattt aatgtcctct gttgattttg atttgtcacc gataaggcta ccagtaaaac
     4561 cactcaacca atatcccaaa gaactgaaat gacctaaagc caccttgtca tttccagtta
     4621 attgaaccat atatttttga acaaatgctg gggaaatact cataataata ccgagtgcta
     4681 atcctcctag taagataaga ggcaagctag taaagccagc aactgataaa atgaccgcaa
     4741 tcatacatgc catatataga gtgtggtgcc ctgttaaaaa aatatattta aatcgagtaa
     4801 aacgagcgat taagatattg aacaccatgc ctgcaaacat aatcagtgca gtagctgagc
     4861 catatgttgt taaagctaca gctacaattg cttcattatt cggcacaacg ccagataaat
     4921 gaaaagcatg ctcaaacatg gtaccaaatg gatttaaaga attttgtaca attcctgcac
     4981 caccagatac aactaagaaa ccaacaaagg tcttaattcc acctttaata atatcaggta
     5041 atttcttctt ctgaagaact aatcctaaaa ttgcaattaa agctactaaa atagctggtg
     5101 tactaacaat atccaatatg aacttcatca tgacgctagc ctcctatata agtccttttt
     5161 cttcacaaag tttagtaatt aattctcgta gttcatccat atcaataata ctatttaaga
     5221 tacgaacatc tccaagatga ctagctgaat cagctagatc acgaccaaca atccaaatat
     5281 cagctgcatt tggatctgct ccacctaaat cataatgttc aacttctaca tctgaaacat
     5341 tcaaatcact caatacagat tcaatattca tctgtaccat aaaacttgaa cctaatcctg
     5401 aaccacaagc tgtaccaatt tttaacatta tctaatcctc ctgtttaatt atcattttaa
     5461 tgtcatcata gttttttgat gatattaaag tttgaacatg atttttatct cttaaaattg
     5521 ttgttaaatg tgacaaagcc tttaaatgac tctcattatc aatggctgca atacaaatca
     5581 acaatcttac ctcttgttct ggattatcca ataaataaat cggttcttcc aaaactaaca
     5641 ttgacattcc tatttcattc acaccttcat ctggccgagc gtgaggaatt gctactccct
     5701 tccctaaatt aataaaaggc ccaaactctt ctactttttg aatcattgcc tcagggtagt
     5761 tctcagttat cttatcttga tccaaaagcg gtttagctgc taaacgaatc gcctccttcc
     5821 atcctaattt ttgcgaacta acctgatagg tttctttggt aatgagttgt tctagcactg
     5881 gtacaatttc ctttctatca tttttttggt aaagataatt ctttaacgcc aatcttaatt
     5941 ccaattcttg tgtaataatt ccatatcttt tgacaatatt cagaatttgt tcaatctcaa
     6001 aatccccata ctctaaattc ggaaaatctt ttaacactag ttctactagt tgtattgctt
     6061 gctcttcagt catcataacc gaaactagat aatttggctt ttctgtctcc acctttatgg
     6121 tagaaaaaac catatcatag tcactactag ctttcacctg taaatcatca atctttgagg
     6181 ttcctataaa ctcaatttga ggaaataatg ctaatagatt ctcttttaac atcaatgaag
     6241 aactaacacc attaggacaa atgattgctg ctttatacca tttttgaggc aaagtatctg
     6301 ctttctttaa ataacctccg aaatggataa caaaatatgc tgtttcacta tcaggtatgg
     6361 gattgtcaat agcgtccatc aagggcatca aagaatcttt gactaattca aataaatcag
     6421 gataatattc tttaacatgc aatacatatt catttgaact aggtaggccg aactttaatc
     6481 tatagtaagc cggtataagg tggcggcgaa gattttctct caatccttcc ctttgtttaa
     6541 aatgtaacaa agaaatatct tccattctac ttataatagc ctctgttaat tgattaaagt
     6601 aaaccggagc aacatctact tcaccttcaa agcaacttga taataaaacg gtgatatagc
     6661 gataatcatc ctcagaaaac accgtatcta taattcccaa atcaaccact gtatccaata
     6721 aaatagtggt tatatcttga ataacaggag atactaatgt ctctgaaaga catactcttt
     6781 caacatccct ttgataccta cacagaatga atactaaacc gaaaaggtaa acttttaatt
     6841 gattaacaat aggtactagc tgtagcttct cataataatc tttaactacc tgatttatca
     6901 aatcataagt taatgaatac ccccaactgg ataaaacata atccaaaccc caaatcccta
     6961 tggaggattc cagcaactca ctaaccattt gaaaagctaa gcggtgctta ttccactctg
     7021 aaccgtgtaa agtataacct tttgctctac tgtaccctag ctccaaatca ttatctaaca
     7081 taatctttct taatgattga atatcagata aggttgtatt cttacttact ttcaaaaagt
     7141 cttggtaatg attattcgat ataaaatcta atcggcaaaa agtgtaaaga tagattaaag
     7201 ctaagcgagt cgactttggt aaaaccaatt catccgactt aataatatct gtcaaagact
     7261 gcttcgtacg atttgataaa ctatagcgac cttgtttttt atccagcact atccctttat
     7321 tagctagata aggcactaaa taatctattc cttctttgac ttcctttata ggtaagctca
     7381 ccttaacaga taattcatat aatgatagct cacaatgatc catcaaagtc atcaaaataa
     7441 ctagtgctct ataatcaaac atttctcttc c
//
LOCUS       DAVKGC010000096         7245 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKGC010000096 DAVKGC010000000
VERSION     DAVKGC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.096"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..607)
                     /locus_tag="VW049_001679"
     CDS             complement(<1..607)
                     /locus_tag="VW049_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9432760.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALENTGHYAFNLLNFLHEQGYKVYTYNPLLIKKFAKSLSLRKTKTDKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVGKHSEYTYQLLTRYPNPQKRLEAGFDKLIEIKRLTAS"
     gene            863..1723
                     /gene="speE"
                     /locus_tag="VW049_001680"
     CDS             863..1723
                     /gene="speE"
                     /locus_tag="VW049_001680"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9432761.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            1720..2979
                     /locus_tag="VW049_001681"
     CDS             1720..2979
                     /locus_tag="VW049_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9432762.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            2979..4106
                     /gene="nspC"
                     /locus_tag="VW049_001682"
     CDS             2979..4106
                     /gene="nspC"
                     /locus_tag="VW049_001682"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9432763.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLEIMDHIVF
                     NSERQLRKHGPRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            4103..5188
                     /gene="aguA"
                     /locus_tag="VW049_001683"
     CDS             4103..5188
                     /gene="aguA"
                     /locus_tag="VW049_001683"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9432764.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            5198..6073
                     /gene="aguB"
                     /locus_tag="VW049_001684"
     CDS             5198..6073
                     /gene="aguB"
                     /locus_tag="VW049_001684"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9432765.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(6254..7063)
                     /locus_tag="VW049_001685"
     CDS             complement(6254..7063)
                     /locus_tag="VW049_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9432766.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHIAAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLTEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         2105 a         1415 c         1630 g         2095 t
ORIGIN      
        1 tagaagcggt caatcgctta atttctatca gcttatcaaa tcctgcctca agccttttct
       61 gaggattagg gtagcgtgtc aagagttggt aggtatattc tgaatgcttt ccaacgattt
      121 tatccaactc aggaaagatg atatcaagac aacgagtgta ttgtactttc caatcagact
      181 gttttttctt gagacgatga atatgtctag ccagtatttt tagttctact tgccgattat
      241 cgtgttgaaa ttgttcacga ttggggtcag aaagaagttt aagagcgatg ccatgagcgt
      301 ctttcttatc cgttttagtt ttgcgaagtg ataatgattt ggcaaatttc ttgatgagca
      361 aaggattgta ggtgtaaact ttatatcctt gttcatgcag gaagttcagt agattaaagg
      421 cataatgtcc ggtattttca agagcgatga gacagtcttg gttgagctgt cgaagagaca
      481 gatctaagag ttcaaaacca gctttattat ttgaaaaagt gagtggttta agaacagttt
      541 ttcctggaac attcaaggct gtaacatcgt gtttattttt agcgacatca atgcccacat
      601 aaagcatggg agtatctcca gatatagtat ttcaagtcta ctgggttatc cacgaacttt
      661 ttgccttgtt accttagacg agataaaacg tctatgcgtt atcaaactca ttaccaattg
      721 aaacaaaaaa ctgtggttag agcctttcgg aaatcgtcaa gcgattggag gaaatgaact
      781 aatccacagt ggcttattcc aagtatacca cttgggcttt ggcagtagct aactgcgcta
      841 aatataatat aaggagaaat agatggattt atggttttct gaagttcata ctccagatgt
      901 caaattgtct ctgagaacag ccaagcaact ttacgctgga aaaagtgaat ggcaggatat
      961 cgaagtcttg gatacgccag cttttgggaa aatactgatt ttaaatggcc atgtcttgtt
     1021 ctcagatgcg gatgatttcg tctacaatga aatgaccgtt cacgttccca tggctgtcca
     1081 cccaaatcca aagaaagtat tggttattgg gggtggtgac ggcggtgttg cccaagtatt
     1141 aaccctctat cctgaactgg agcaaattga tattgtggaa ccggatgaga tgttggtcga
     1201 ggtctgtcgt gagtatttcc cagactttgc tgcagggcta gatgatcctc gtgttaccat
     1261 ttactaccaa aatgggctac gctttttgcg aaactgcgaa gatgattacg atattatcat
     1321 caacgatgcg acagatccat ttggccatac ggaaggactc tttaccaagg aattctacgg
     1381 caatagttat cgagctctga aggaagacgg catcatgatt taccagcatg ggagtccctt
     1441 ctttgacgag gatgagtcgg cctgccgaag catgcaccgc aaggtcaatc aagcctttcc
     1501 aatcagtcgg gtttatcagg cccatattcc aactagccca gctggctatt ggttgtttgg
     1561 atttgcatcg aaaaaatacc accctgtcaa agattttgac aaggaaggct ggaaaaaacg
     1621 ccagcttttc acagaatact acactgcaaa cttacacgtg ggagccttta tgttgcccaa
     1681 gtatgttgag gacattttag aagaagagga aggaaaaaaa tgagtcgttt actagttatt
     1741 ggttgtgggg gcgttgccca agttgctatt tcaaagattt gtcaagatag cgaaacattt
     1801 acagagatta tgattgctag ccgtaccaag tcaaaatgcg atgacttgaa agcgaagcta
     1861 gaaggcaaaa caagtactaa aattgaaact gcagcacttg atgctgacaa ggttgaagaa
     1921 gtgattgccc tgattgaaag ctacaaacca gaagctgttt tgaatgtagc tctgccttat
     1981 caagatttaa ccattatgga tgcttgtttg gcaacaggtg ttcactatat cgatacagcc
     2041 aactacgaag cagaagacac agaagaccct gagtggcgtg ctatctacga aaaacgttgt
     2101 aaggaacttg gttttacagc ctactttgac tactcatggc agtgggctta tcaagagaaa
     2161 ttcaaagaag caggcttgac tgctcttctt ggttctggtt ttgacccagg tgtaactagt
     2221 gtcttttcag cttatgccct caaacactat tttgatgaaa tccattatat cgacatttta
     2281 gactgtaatg gcggtgacca cggttatcca tttgcaacca actttaatcc agaaattaat
     2341 ctccgtgagg tttctgcgcc aggttcttac tgggaagatg ggaaatgggt cgaagtcgaa
     2401 gctatgtcta tcaagcgtga gtatgatttc cctcaagttg gacaaaaaga tatgtatctc
     2461 cttcaccatg aagaaatcga atcattggcc aagaacattc caggtgtcaa acgcattcgt
     2521 ttctttatga cttttggtca atcttacttg acgcacatga aatgtcttga aaatgttgga
     2581 ctccttcgta cggataccat taactttaac ggccaagaaa ttgttccaat tcaatttttg
     2641 aaagccttgc ttccagatcc tgccagtctt gggccacgta cagtcggaaa aaccaatatt
     2701 ggatgtatct ttacaggtgt caaagacggt gtcaaaaaga ctatctatat ctacaatgtc
     2761 tgcgaccatc aggaatgtta cgcagaggtt ggttcgcaag ctatttctta tacgacagga
     2821 gttccagcca tgattgggac aaaattagtc atgaacggaa cttggaaaca agctggagtg
     2881 tataaccttg aggagttaga tccagatcca ttcatggaag ctttgaatga gtatggtttg
     2941 ccatgggttg tggttgaaaa tccacaaatg gtggactaat gaagttagaa caagtaccaa
     3001 caccagccta tgttattgac ttggccaagt tagaagctaa ttgccgcatt ctacaatatg
     3061 tacaagaaga ggccggttgc aaggtcttgc ttgcccagaa ggcatattcc ctctacaaaa
     3121 cttatccctt gattagccag tatctatcag gtacgacagc tagtggactc tatgaggcca
     3181 aattggcaag ggaagaattt cctggtgaag tccatgtatt tgcgcctgct ttcaaggatg
     3241 cagacttgga ggaattgcta gagataatgg accatatcgt ctttaactca gagagacagt
     3301 tgcgtaaaca cggtccgcgt tgtcgagagg ctggtgtcag tgttggtttg cgcctcaacc
     3361 ctcagtgttc aactcaagga gatcacgcgc tctatgaccc ttgtgcacca ggttctcgct
     3421 ttggagttac tatagacaag attccgagtg atttgctaga tttggttgac ggacttcatt
     3481 ttcataccct ttgcgagcag ggagcagatg atttacaaac aactttgaaa gcagtagaag
     3541 aacagtttgg tccctactta catgaggtaa aatggctcaa tatgggtggt ggtcatcata
     3601 ttacaagaga aggttacgat gtggatttgc tgatttcaga aatcaagcgt atccgaaaaa
     3661 cttacaatct tgaaatctat atcgagcctg gtgaagccat tgcgcttaat gcgggttatt
     3721 tagcaactga ggtattagat attgtagaaa acggtatgga aatcttggtt ttagacgcct
     3781 ctgcgacctg ccatatgcct gatgtacttg agatgcccta tcgtccacct ttgagaaatg
     3841 gctttgagtc acaggaaaaa gcccatacct acagactttc ttctaatacc tgtctgacgg
     3901 gcgatgtgat tggtgattat agttttgaaa atccagtcca aatcggagac agactttatt
     3961 ttcaagacat ggccatttat tcttttgtca aaaataatac ctttaatggt attggattgc
     4021 caagtctcta tctcatggac gaacagggag actgtagctt actcaaagct tttggctatc
     4081 aagactttaa agggagatta tcatgatgga cagtccaaaa aaattaggct atcacatgcc
     4141 agcagagtac gaaccccatc atggtaccct catgatatgg ccgactcgac caggatcatg
     4201 gccttttcaa ggaaaggctg ctaaaagagc atttactcag atcatcgaga ccatagcaga
     4261 aggggaaaga gtctatcttt tggtggagca ggcctatcta tctgaagccc aatcctatct
     4321 tggagacaag gttgtttatt tagacattcc caccaatgat gcctgggcgc gtgatactgg
     4381 cccaaccatt ctcgtcaatg ataaaggtaa gaaattagcc gtggattggg cctttaatgc
     4441 ttggggaggc acctatgatg gtctttatca agattatgaa gaggatgacc aagtagccag
     4501 tcgttttgct gaggccttgg aaaggcctgt ctatgatgct aaaccttttg tactggaagg
     4561 aggcgcaatc catagcgatg gtcaaggaac tattctcgta actgaaagtt gcttgcttag
     4621 tcctggtcgc aatcctaacc tgactaaaga ggagattgaa aacacattat tagaaagtct
     4681 tggtgctgaa aaagttattt ggcttcctta tggtatttat caggatgaaa ccaatgaaca
     4741 cgtcgataat gttgctgcct ttgttggtcc tgctgagctt gttttggctt ggacagatga
     4801 cgaaaatgat ccccagtatg ccatgtcaaa agcagatctc gaactcttag aacaggaaac
     4861 agatgcaaaa ggttgtcact tcaccattca taaattgcct atccctgcag ttcgacaagt
     4921 tgtgacagaa gaagatttgc caggctacat ctatgaagaa ggagaagaaa agcgatacgc
     4981 aggtgaacga ctagcagctt cctacgtaaa cttttatatc gccaacaagg ctgtcttggt
     5041 tccacagttt gaggatgtaa acgaccaagt ggccttagat atcctcagca agtgtttccc
     5101 agaccgtaaa gttgtcggaa taccagccag agatattctc ttaggtggtg gcaatatcca
     5161 ctgtatcacc caacaaattc cagaatagga gaaaaagatg agaaatgtaa gagttgcaac
     5221 cattcagatg caatgcgcta aggatgtggc aacaaatatc caaaccgcag agcgtttagt
     5281 acgtcaggct gctgagcaag gagcccaaat tattctcttg cccgagttgt ttgaacatcc
     5341 ctatttctgt caggaacgtc agtatgacta ctaccagtat gcccaatctg tagcggaaaa
     5401 tactgccatt cagcatttta aggtgattgc taaggaacta caagttgttt taccaatcag
     5461 tttctatgaa aaagatggta atgtcttgta taactctatt gccgtcattg atgcagatgg
     5521 ggaagtgctg ggcgtttatc gaaagaccca cataccagat gaccattatt atcaagaaaa
     5581 attctatttc acgcctggta acactggttt caaggtctgg aatactcgct atgctaagat
     5641 tggtatcggt atctgttggg atcaatggtt ccctgaaaca gcgcgctgtc ttgcattgaa
     5701 tggtgctgaa ttgctctttt atccaacagc catcggttca gagccgattt tggatacaga
     5761 tagttgtggt cactggcaac gtactatgca agggcacgca gcagcgaata ttgttccagt
     5821 catcgcagcc aatcgttatg gtttagagga ggttactcct agtgaggaaa atggcggaca
     5881 gagttccagt cttgacttct acggttcctc ctttatgacg gatgaaacag gagctattct
     5941 agaacgagct gaaagacaag aagaagctgt tctgttagct acttatgacc tagacaaggg
     6001 agcgagtgaa cgcctaaact ggggcttgtt tcgagataga agaccagaaa tgtatagaca
     6061 aattacagat tagtgtggga gaaatgagag attcattctg ctagactaac ttcttattag
     6121 caactataag atactatggc atctagtaaa tcgattttta tgattcgcta ttcttgtcta
     6181 ttgattagtc cgtattttaa aatattagca aaaaagcaaa tagcagtaac ttctgtctat
     6241 ttgcttttct tttttataga atatatttct caatagcacg cgcaacgccg tcttcttcgt
     6301 tgcttgaggt aacggcatcc gcaagagatt tgacataatc gctggcattt cccattgcaa
     6361 tcccaagccc tgcaaactgg agcatttcga tatcgttatt agcatcgccc atggccataa
     6421 tctctgagga atcaatcttc aaaatctcag ctagtcgtga aagagcagta gcctttgtcg
     6481 ttccaagtgg cattgcttca taaatgacag gctgcgaacg aactccactg aatcgttggc
     6541 aaagctcttc agcaaaacgc tgctcaaaat cgtctgtttg ttcttttgtt cctaaaaaca
     6601 taccttggaa catccggaac tttccactag tcgcttcctc aagagaaatt tcagtcaggt
     6661 ctgaaaatac tagtttagca tcattttcaa taacttgatt gggcttgcca ccgagaacaa
     6721 aataatgtga ctcgtcaaaa agtgtcaact gaacatcact cttttcagta aggtcataga
     6781 ggtattcgat gtcagctgga ctcagttctt gccagtcaac caagccccag tcgctagtct
     6841 ggtgagttga acaaccgttg ttaacaatca catattcgtt ctggaggtcg agtcccagtt
     6901 ttttgtagta ggggaggaca ccgaaaaggg ggcgacccgt acagaggacc agtttgacac
     6961 ctttttcaat ggcttggtga atagcagcga tgtgagcctg tgggatttcc ttggcttcat
     7021 tgaggagggt tccatccata tccaaggcta gtagtttaat cataggtctt ccttctttat
     7081 cttttggtat tattatagca tattttggag aagaaattga tagaaagctt gagactaatt
     7141 gattttatag tttaagatgt tttgatgaca attcatgatt tgaagaggat atttcgcaaa
     7201 gatatgctat actatgtttg tcaatgttgc aactagacaa attaa
//
LOCUS       DAVKGC010000097         7181 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKGC010000097 DAVKGC010000000
VERSION     DAVKGC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.097"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..89)
                     /locus_tag="VW049_001686"
     CDS             complement(<1..89)
                     /locus_tag="VW049_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9432767.1"
                     /translation="MCYNLFMAYSIDFRKKVLSYCERTGSITEA"
     gene            324..1769
                     /gene="cps4A"
                     /locus_tag="VW049_001687"
     CDS             324..1769
                     /gene="cps4A"
                     /locus_tag="VW049_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU9432768.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVAAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGIDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1771..2502
                     /locus_tag="VW049_001688"
     CDS             1771..2502
                     /locus_tag="VW049_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEU9432769.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAEAYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYNALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            2511..3203
                     /gene="cpsC"
                     /locus_tag="VW049_001689"
     CDS             2511..3203
                     /gene="cpsC"
                     /locus_tag="VW049_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU9432770.1"
                     /translation="MKEQNTLEIDVLQLFRVLWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKSGLTNQDLQAGSYLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKGLANKIKVTVPADTRIVSVSVNDRVPEEASRIANSLREVAAKKIISITRVSDVT
                     TLEEARPAISPSSPNLKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIEDTLQMT
                     LLGVVPNLNKLK"
     gene            3213..3896
                     /gene="cpsD"
                     /locus_tag="VW049_001690"
     CDS             3213..3896
                     /gene="cpsD"
                     /locus_tag="VW049_001690"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEU9432771.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVS
                     PGEGKTTTSVNIAWSFARAGYKTLLIDGDIRNSVISGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTATGEANKRDVQKAQQQLKQTGKLFLGVVFNKLDISVDKYGVY
                     GFYGNYGKK"
     gene            3912..5279
                     /locus_tag="VW049_001691"
     CDS             3912..5279
                     /locus_tag="VW049_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9432772.1"
                     /translation="MDEKGLKIFLAVLQSIIVILLVYFLSFVRETELERSSMVILYLL
                     HFFVFYFSSYGNNFFKRGYLVEFNSTIRYIFFFAIAISVLNFFIAERFSISRRGMVYF
                     LTLEGISLYLLNFLVKKYWKHVFFNLKNSKKILLLTVTKNMEKVLDKLLESDELSWKL
                     VAVSVLDKSDFQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFEKMG
                     MDVTVNLKAFDKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVISKRILDICGATIGLIL
                     FAIVSLVLVPLIRKDGGPAIFAQTRIGKNGRHFTFYKFRSMQIDAEAIKEQLMDQNTM
                     QGGMFKIDNDPRVTKIGRFIRKTSLDELPQFWNVFIGDMSLVGTRPPTVDEYDQYTPE
                     QKRRLSFKPGITGLWQVSGRSKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFM
                     RDGAK"
     gene            5310..6482
                     /locus_tag="VW049_001692"
     CDS             5310..6482
                     /locus_tag="VW049_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU9432773.1"
                     /translation="MKKSVYIIGSKGIPAKYGGFETFVEKLTAFQQDKTIQYYVACMR
                     ENSAKSGTTEDVFEHNGAICYNVDVPNIGPARAIVYDIAAINRAIKIAKENKDEDPIF
                     YILACRIGPFIHGIKKKIQEIGGTLLVNPDGHEWLRAKWSAPVRRYWKISEGLMVKHA
                     DLLVCDSKNIEKYIQEDYIQYQPKTTYIAYGTDTTRSTLNSSDEKVRAWFKEKNVSEN
                     EYYLVVGRFVPENNYESMIRGFLASSSKKDFVLITNVEQNKFYNQLLASTGFDKDPRV
                     KFVGTVYDQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     QSAIYWKKDELSQVIEKVEQFDAKIIDELDRQSNQRIVDFFTWEKIVTDYEKLFKK"
     gene            6479..>7181
                     /locus_tag="VW049_001693"
     CDS             6479..>7181
                     /locus_tag="VW049_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9432774.1"
                     /translation="MKILHYTLGFQPQRTGGLVKYAEDLMLEQIAQGYQVAALYPGRI
                     KFFSKKIEIIKETSRQFECYELLNSLPMALFGGISDPTAFLTPCDKNVYRTFLEKVQP
                     DIIHIHSFMGLHKEFLEIAKSLNIRVVFTSHDYYGLAPVPHFYFNGVDYSDKSTNLTW
                     NIMSSNALSVKKLRLFQVSFYPTIRKLLKLLGKNPKSKKNLVIRDVIEEQDYSELRYY
                     YNEMFHLIDGYLFNSR"
BASE COUNT         2351 a         1092 c         1465 g         2273 t
ORIGIN      
        1 gcttctgtta tactacctgt tcgctcacaa taagagagaa cttttttacg aaaatctatt
       61 gaatatgcca taaaaagatt atagcacatt gtgtactata gtagattgaa actagaatag
      121 tacacctctg cttctaaaat attgttagaa atcgatttga ctgtcctgat cgatttgtca
      181 tgttcttatt tcattttact atatttttgg ttcgcgggaa gtctactaag atacttaaag
      241 atgcagatag tgaaaataaa ggtgtagaca ttaccgtaaa aaagtgatat aatcgtatga
      301 tgttcaatgt ataggtgtta atcatgagta gacgttttaa aaaatcaggt tcacagaaag
      361 tgaagcgaag tgttaatata gttttgttga ctatttattt attgttagtt tgttttttat
      421 tgttcttaat ctttaagtac aatatccttg cttttagata tcttaatcta gtggtaactg
      481 cgttagtcct actagttgcc ttggtagggc tactcttgat tatctataaa aaagctgaaa
      541 aatttactat ttttctgttg gtgttctcta tccttgtcag ctctgtgtcg ctctttgcag
      601 tacagcagtt tgttggactg accaatcgtt taaatgcgac ttctaattac tcagaatatt
      661 caatcagtgt cgctgtttta gcagatagtg agatcgaaaa tgttacgcaa ctgacgagtg
      721 tggcagcacc gactgggact gataatgaaa atattcaaaa actactagct gatatcaagt
      781 caagtcagaa taccgatttg acggtcaacc agagttcgtc ttacttggca gcttacaaga
      841 gtttgattgc aggggagact aaggccattg tcctaaatag tgtctttgaa aatatcatcg
      901 agtcagagta tccagactac gcatcgaaga taaagaagat ttataccaag ggattcacta
      961 aaaaagtaga agctcctaag acgtctaaga atcagtcttt caatatctat gttagtggaa
     1021 ttgacaccta tggtcctatt agttcggtgt cgcgatcaga tgtcaatatc ctgatgactg
     1081 tcaatcgaga taccaagaaa atcctcttga ccacaacacc acgtgatgcc tatgtaccaa
     1141 tcgcagatgg tggaaataat caaaaagata aattaaccca tgcgggcatt tatggagttg
     1201 attcgtccat tcacacctta gaaaatctct atggagtgga tatcaattac tatgtgcgat
     1261 tgaacttcac ttctttcttg aaaatgattg acttattggg agggatagat gttcataatg
     1321 atcaagagtt ttcagctcta catgggaagt tccatttccc agtagggaat gtccatctag
     1381 actctgagca agctttaggt tttgttcgtg agcgctactc cctagcagat ggcgatcgtg
     1441 accgtggtcg caaccaacaa aaggtgattg tggctatcct tcaaaaatta acgtcaaccg
     1501 aagcactgaa aaattatagt acgatcattg atagcttgca agattctatc caaacaaata
     1561 tgccacttga gaccatgata aatttggtca atgctcagtt agaaagtggt ggaacttaca
     1621 aagtgaattc gcaagacttg aaaggtacag gtcggatgga tcttccttct tatgcaatgc
     1681 cagacagtaa cctctatgtg atggaaatag atgatagtag tttagctgta gttaaagcag
     1741 ctatacagga tgtgatggag ggtagatgaa atgatagaca tccattcgca tatcgttttt
     1801 gatgtagatg acggtcccaa atcaagagag gaaagcaagg ctctcttggc agaagcctac
     1861 agacaggggg tgcgaaccat tgtctctacc tctcaccgtc gcaagggcat gtttgaaact
     1921 ccggaagaga agatagcaga aaactttctt caggttcggg aaattgcaaa agaagtagca
     1981 gatgatttag tcattgctta tggcgcagag atatactata ctctggatgc tctagaaaag
     2041 ctagaaaaaa aagaaattcc tacccttaat gatagtcgtt atgccttgat tgagtttagc
     2101 atgcatactt cctatcgtca gattcatacg ggattgagca atattttgat gttgggaatc
     2161 acgccagtaa ttgctcatat tgaacgttat aatgctttag agaataacga aaaacgtgtt
     2221 cgtgaactga ttgatatggg gtgctatact cagataaata gttatcatgt ttcaaaacct
     2281 aagttctttg gtgaaaaata taaattcatg aaaaagagag ctcggtattt tttggaacgt
     2341 gatttagttc atgtagttgc aagtgacatg cacaatttag acagtagacc tccatatatg
     2401 caacaggcat atgatatcat tgctaagaaa tatggagcga aaaaagcgaa agaacttttt
     2461 gtagataatc ccagaaaaat tataatggat caattaattt aggagaaaat atgaaggaac
     2521 aaaacacttt ggaaatcgat gtattgcaac tattcagagt tttatggaaa aggaagttgg
     2581 tcattttatt agtggcaatt ataacttctt cagttgcttt tgcctacagt acttttgtta
     2641 tcaaacctga gtttactagt acgactcgga tttatgtagt taaccgtaat cagggagaga
     2701 agtctggttt aaccaatcaa gacttgcagg caggatcgta cttggttaaa gactatcgtg
     2761 aaattatcct atcgcaggat gttttggagg aagttgtttc tgatttgaaa ctagatttga
     2821 cgccaaaagg tttggctaat aaaatcaaag taacagtacc agctgatacc cgtattgtct
     2881 ctgtttcagt taatgatcga gttcctgaag aggcaagccg tatcgctaac tctttgagag
     2941 aagtagctgc taaaaaaatt atcagtatta ctcgtgtttc tgatgtgaca acactggagg
     3001 aggcaagacc ggcgatatca ccgtcttcgc caaatcttaa acgcaataca ctaattggtt
     3061 ttttggcagg ggtgattgga actagtgtta tagttcttct tcttgaactt ttggacactc
     3121 gtgtgaaacg tccggaagat atcgaagata cactacagat gacacttttg ggagttgtac
     3181 caaacttgaa taagttgaaa taggagagag agatgccgac attagaaata gcacaaaaaa
     3241 aactggagtt cattaagaag gcagaagaat attacaatgc cttgtgtaca aatatacagt
     3301 tgagcggaga taaactaaaa gtaatttccg ttacttctgt tagcccgggg gaaggaaaaa
     3361 caactacttc cgtaaatata gcatggtcgt ttgcgcgtgc aggctataaa actcttttga
     3421 tcgatggcga tattcgaaat tcagtcatat caggattttt taaatctcgt gaaaaaatta
     3481 cagggctaac agaattttta tctgggacag ctgatttatc tcacggttta tgtgatacaa
     3541 atattgaaaa tttatttgta gttcaatcgg gctctgtatc accaaaccct acagccttgt
     3601 tacaaagtaa aaattttaat gatatgattg aaacattgcg taaatatttt gattatatca
     3661 ttgttgatac agcacctatt ggaattgtta ttgatgcggc aattatcact caaaagtgtg
     3721 atgcgtccat cttggtaaca gcaacaggtg aggcgaataa acgtgatgtc caaaaagcgc
     3781 aacaacaatt aaaacaaaca gggaaactgt tcctaggagt tgtttttaat aaattagata
     3841 tctcggttga taagtatgga gtttacggtt tctatggaaa ttatggtaaa aaataactta
     3901 ggaaagattt tatggatgaa aaaggattga aaatttttct ggcagtatta cagagtatta
     3961 ttgtcatttt attggtttat tttcttagct ttgttagaga gacagaactt gaacgttctt
     4021 cgatggttat actatacctt ctccactttt ttgtattcta ttttagttcc tatggtaaca
     4081 atttttttaa aagagggtac ctagttgagt ttaatagtac tataagatat atttttttct
     4141 ttgcaatagc tataagtgta ttaaactttt ttatagcgga acggtttagc atctctagaa
     4201 gaggaatggt atacttctta actttagaag gaatatcctt atacttgtta aatttcttag
     4261 taaagaaata ttggaagcat gtgtttttta atctaaaaaa tagcaagaaa attttactgt
     4321 taacagtaac gaaaaatatg gaaaaagttc ttgataaatt gctagaatct gatgaacttt
     4381 catggaaatt ggtagcagta agtgttttgg ataaatctga ttttcaacat gataaaatac
     4441 ctgtaattga aaaggaaaaa attattgaat ttgcaacgca tgaagttgtg gatgaggtgt
     4501 ttgtcaatct tccaggagag agctacgata ttggagaaat tatctctagg tttgagaaaa
     4561 tggggatgga tgtaactgta aatcttaaag catttgataa gaatttgggt cgcaataaac
     4621 aaattcatga gatggtagga ttgaatgtag tcactttctc tacaaatttt tataaaacta
     4681 gtcatgtgat ttcaaagaga attctcgata tttgtggtgc cactattggc cttattcttt
     4741 ttgctatagt tagtctagtt ttagttccat tgattcgtaa agatggcgga ccagctattt
     4801 ttgctcaaac tcgtataggg aaaaatggtc gacattttac cttttataaa ttccgttcga
     4861 tgcagatcga tgctgaagct atcaaagaac agttgatgga tcaaaatacg atgcaaggtg
     4921 gtatgtttaa gatagacaat gatcctcgtg ttacaaaaat tggtcgcttt attcgtaaaa
     4981 ccagtttaga tgagttaccc cagttttgga atgtctttat aggagatatg agtttggtgg
     5041 gaacacgtcc acctacagta gacgagtatg atcagtatac tccagaacag aaacgtcgac
     5101 tcagctttaa acccggtatt acaggtttat ggcaggttag cggccgtagt aaaataaccg
     5161 attttgacga tgttgtaaaa ttagatgtgg cttatattga taattggaca atctggaaag
     5221 atattgaaat tttgcttaaa actgttaaag ttgtatttat gagagatgga gcgaagtaat
     5281 ttctgtatat ccatcatatt aggagagaaa tgaaaaagtc agtttatatc attggttcaa
     5341 aaggaattcc tgctaagtat ggaggatttg aaacttttgt tgaaaaatta acagccttcc
     5401 aacaagataa gactatccaa tattatgtgg cttgtatgcg tgaaaactct gcaaaatcag
     5461 ggactactga ggatgttttt gaacataatg gtgctatctg ttataacgtc gatgttccta
     5521 atattggtcc agcgcgagcc atagtatatg atatcgcggc aattaacaga gctattaaaa
     5581 ttgccaaaga aaataaggat gaagatccaa tcttctatat tctagcttgt cgaatcggtc
     5641 cgttcatcca tggaattaag aaaaaaatcc aggagatagg tgggactctt ctagttaatc
     5701 cagatggtca cgagtggtta cgagctaagt ggagcgctcc agttcgtcgt tattggaaaa
     5761 tttccgaagg tcttatggtc aaacatgcag atcttttagt gtgtgatagt aagaatattg
     5821 aaaaatatat ccaagaagat tatatacagt atcaacctaa gacaacctat atcgcttatg
     5881 ggacagatac aacacgctcc acattaaata gcagtgatga aaaagtgcgt gcttggttca
     5941 aggagaaaaa tgtttctgaa aacgagtatt acctagttgt aggacgtttt gttccagaaa
     6001 ataattatga gtctatgata cgtggtttct tggcgtctag ctctaagaag gattttgtcc
     6061 taatcacaaa tgtagaacag aataaatttt ataatcagct attagcaagt actggatttg
     6121 ataaagatcc acgagtgaaa tttgttggaa cagtctacga ccaagaactt ctgaagtata
     6181 ttcgagaaaa tgcttttgct tacttccacg gacatgaagt tggtggaaca aatccttcgt
     6241 tacttgaagc attagcatcc acaaagttaa acttactact cgatgttggt tttaaccgcg
     6301 aagttgggga gcaaagtgcg atctattgga aaaaagatga gctttcccaa gtaatcgaga
     6361 aagttgaaca atttgatgca aaaattattg atgagttaga tagacaatca aatcagagaa
     6421 ttgtggattt tttcacttgg gaaaagattg tcacagacta cgagaaatta tttaaaaaat
     6481 gaaaatatta cattatactt taggatttca accccagcga acaggtggtt tggttaaata
     6541 tgctgaggat ttaatgctag agcaaattgc tcaaggttat caagtggctg ccctatatcc
     6601 aggaagaatt aagttctttt caaaaaaaat agaaataatt aaagagacgt caagacagtt
     6661 tgagtgttat gagctgctta atagcttacc tatggcttta tttggaggga tatccgatcc
     6721 aactgctttc ctgacacctt gtgacaaaaa tgtttatcgt acttttttag aaaaagtaca
     6781 acccgacatt atacatattc actcgtttat gggattgcat aaagaattcc tcgaaattgc
     6841 taaaagcttg aatattagag tggtttttac gagccatgat tattatggat tagcacccgt
     6901 acctcatttc tattttaatg gggtggatta tagtgataaa agtacaaact taacatggaa
     6961 tattatgtct tccaatgctt taagtgtgaa aaaactaaga ctttttcagg tttcatttta
     7021 tcctactatt cgtaaacttt tgaaactatt agggaaaaat ccaaaatcta aaaagaattt
     7081 agttattcga gatgttattg aggagcaaga ttatagtgag cttcgatact attataatga
     7141 gatgtttcac ttaatagatg gttatttgtt taatagtaga c
//
LOCUS       DAVKGC010000098         7151 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKGC010000098 DAVKGC010000000
VERSION     DAVKGC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.098"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(58..384)
                     /locus_tag="VW049_001694"
     CDS             complement(58..384)
                     /locus_tag="VW049_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9432775.1"
                     /translation="MVEYISRKYFLTEEEFQAEKICNQSLVELFQKKYSWHSLNDFGS
                     STYDKNYASIFYEYWRSFLTVDKLVENLGSVQAVLDSYHLWANTEKTFPLLDWFVQQK
                     LIEKEI"
     gene            complement(351..635)
                     /locus_tag="VW049_001695"
     CDS             complement(351..635)
                     /locus_tag="VW049_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9432776.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMTLISGSKRGWLNILVASIS"
     gene            complement(908..1750)
                     /locus_tag="VW049_001696"
     CDS             complement(908..1750)
                     /locus_tag="VW049_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9432777.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYHAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(1864..3255)
                     /locus_tag="VW049_001697"
     CDS             complement(1864..3255)
                     /locus_tag="VW049_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9432778.1"
                     /translation="MDKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIELLKKEKDDQLSEGNQKEYFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKSFSDQDLSAWNFDYKDS
                     QIILYPSPVLENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(3490..3867)
                     /locus_tag="VW049_001698"
                     /pseudo
     CDS             complement(3490..3867)
                     /locus_tag="VW049_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            4082..5059
                     /locus_tag="VW049_001699"
     CDS             4082..5059
                     /locus_tag="VW049_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9432779.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQIPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            5056..6138
                     /locus_tag="VW049_001700"
     CDS             5056..6138
                     /locus_tag="VW049_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9432780.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(6202..>7151)
                     /locus_tag="VW049_001701"
     CDS             complement(join(6202..6754,6753..>7151))
                     /locus_tag="VW049_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9432781.1"
                     /translation="LMGKSYKEIIEFLDCNQTTIWQNVKKYEEFGLDSLLQETRGGRN
                     HAYMTVEEEKAFLARHLKAAEAGEFVTIDALFQAYKKELGRSYTRDAFYQLLKRHGWR
                     NITPRPEHPRKADAQTIVASKNKISIQEEKKKRFKTSRPFHKVRLMYQDEAGFGRISK
                     LGSCWSPIGVGPHVHSHYIREFRYCYGAVDAHTGESFFLRAGGCNTEWMNVFLEELSQ
                     AYPDDYFLLVMDNAIWHKSSTLKIPTNIGFAFIPPYTPEMNPIEQVWKEIRKRGFKNK
                     AFRTLEDVMNQLQDVIQGLEKEVIKSIVNQRWTRMLFESR"
BASE COUNT         2139 a         1573 c         1217 g         2222 t
ORIGIN      
        1 tctgccactg atgcagtgaa atgtcaattt cttagacatt gtttactcct ttagttttta
       61 tatttctttt tcaattaatt tctgctgaac aaaccaatct aacaagggaa aagttttttc
      121 tgtatttgcc cataaatgat aagaatctaa gaccgcttgt acactaccta aattttctac
      181 caacttatct actgtcaaaa agctgcgcca gtattcataa aaaatacttg catagttctt
      241 atcataagtc gaagaaccaa aatcattcaa tgaatgccaa ctatacttct tctgaaaaag
      301 ttctacgaga gattgattac aaattttttc cgcttgaaat tcctcttctg tcaagaaata
      361 cttgcgacta atatattcaa ccatcccctc ttcgaaccag atataagagt cataaccatt
      421 cgtctattat ttgtataggc tggaacagcg ctttccctaa taatctgtgt agcagcatta
      481 aaatctgacc aaataacggc ttgcggtaaa tcacagactg caagttcatt ctttaaaaaa
      541 gattgataat ctttcaactt ttcagtattt cttactacaa aatcacgaaa tgtagctagt
      601 tgactatcgt cttttacaag ataaaggttg tccacggctc tcctttcttt cacacaatag
      661 actgaataag caaaagaacc gcgtccgaca atcctaggtt gatttaacct aggggcgtca
      721 gaaacgcggt tccaccctaa tttattactt cattgttttt gaaaatacta cagaaagcgc
      781 cagctcatta ttttgcccta cttggctccc actatcccaa gctcgcttga agaacgccat
      841 aagactttct ttcctgctga ctattatatc aaaaattaga ataatgtaca attcttttta
      901 tgattttcta gaaatccaca tcatcaactc gtggagcacc cgattgaaca gcaatcgttt
      961 ttaaggtttc tctctcctca tgactcagtt caattccaaa gcaatcaaga ttagcttgaa
     1021 tacgagaggt tgtgacagat tttggaagtg gtaaaaatcc ttctgccaag ctccaggcca
     1081 aggctatctg agcaatcgat tttccgtgat ttgctgcgat ttcttgcact tgcttgctat
     1141 caaatagttc tccctgacca aaaggccccc aagcttccaa taaaattccc ttttcacgac
     1201 agtaagctac gacttgatct tgatacacac ctggcgccaa gcgaacttga ttgaccgcag
     1261 gaacgatagt tgcagtttca agcaaggcat ccaaatgatg gggaagaaaa ttgctaacgc
     1321 cgatagcacg gattttccct tcttgataga ggtcttccat cgctctccaa acttccgcat
     1381 tgcgagtttt ccatgcgtca ttttctctga gcggttttgg gttcggccaa tgaatcaaat
     1441 acaaatccaa ataatccaag cccagttttt ctatagattt ttccaaagct tgacgagttt
     1501 gctcataggt ttgctgacta ttccaaagct tggtagttac gaacatttct tcacgtggaa
     1561 ctccgctatc tttgattgct tgaccaacac tttcttcatt ctgataaatc gccgccgtat
     1621 caatatgacg ataaccagcc ttcaaggctt ctaacactgc atgataggct tcttctccat
     1681 ccttagcctt aaaagttcca aatcccaata ctggaatttc tactccatta tttaattgat
     1741 aggtattcat atgcttctcc ttctatttta ttcatcaatt atagcataag acatttttta
     1801 agtagtacta tcttctgtaa aaaataagtc tgtcaaatat ctaacagacc tattttttct
     1861 tgcttattca tcacgactat agtacagctc atgagctttt aggcgagtat tgagcatctc
     1921 tggtatggtc acaaaggtat aaccttgatt tttcaaatac tcaatgaccc ttggcaaggc
     1981 attgactgtc ggactgtgaa tatcatgcat caaaacgata gagccgttag ctacttgatg
     2041 ctgaatttct gtcaaaatag atgcttcatt tttactcttc cagtccagac tatccacatc
     2101 ccacatgata aagctcaaat ccaagctatt gcgaatatca tctgtaatag caccataagg
     2161 tggacgcatg agtttagaac tagaacccag cactttagtt agcacatcct cagtatcagt
     2221 aatctgcttt ttagcttcat caagagagag ttgcgagaga atcggatggc tccaactatg
     2281 gtttccaaca acatgacctt cagattttat cctcttcacc aagtcctcat tcccagaaac
     2341 atttttccca agcacaaaga aagtcgcttt aatatcatat ttagctaggg tctctaatac
     2401 ctgcggggtc gttgctggat ttggaccatc atcaaaggtt agagcgacaa ctttttgata
     2461 tttcttatca aagtaagatt gatacaaggc cgcatctttt tcgagtaagt acgaagattg
     2521 gataacatca aagaaagcag atactggcaa ggctatctct tctaaatttt caagcacagg
     2581 acttggataa aggataatct gactatcctt gtaatcaaaa ttccatgcag acaagtcttg
     2641 gtcagagaag ctttttacaa tctgctcact ctggtcttgc tctatttttt tatcctctat
     2701 gaaggaggtc aactctttta tcagctgttc cttagcctta ctagcatctg aaaacagttg
     2761 gtcaagtgta aaaggttgcc catcttctgt taagtgaacc tttcctagac tggtcttttc
     2821 agtctcttca atcttaaaag caactaaatc atagatttgt ttggtcacat tacgattaac
     2881 gactcccttt aaacctgact cttcttgctc tgtatagtag aaaacaagat tgtccttact
     2941 ttctagcttg tccttaacat cttgatttat cagctcccta acagaggaaa tcactttctc
     3001 cccttggaga gggtaatagg caatcacttc ggcttgcccc tgacgaaaat actccttctg
     3061 atttccctca ctcaattgat catctttctc ttttttgagc aattcaatct tttgctcaaa
     3121 acttttttgc tggtagatct taaaagcaat aaatccgcct aataggcaaa tagaaattgc
     3181 taaaatacca atcaaagcca ataatacatg tctcgttttc ccgtgtctgc cacgtcccag
     3241 tctactttta tccatattta tatcataaca aatttgagca ataatttcaa tgatacaatt
     3301 tggcaatcca caattcctct agcaagccaa agcatcttac atcaactcca acatccgtat
     3361 ctgtagagat tatgtagttt tctatctatg gaatttctag tagattgaag ctagaatagt
     3421 agacctatac ttctaaaaca ttgttagaaa tcgatttgat tgtcctgatc gatttgtcct
     3481 gttctcattt cattttacta taaaactatg aaaagctagg tcatctgaga agtaattact
     3541 atcgacaatt gcgatagcgt atactagtgc gatttgtttg tagctctggt gggtatcacc
     3601 ttcacgttgg cgaatttttt ctaggttttg attaacctga ctgcacagat aaggccatag
     3661 gcgattgatg aaaaaattct gatgatgatc ctgaatttcg atgataactt ggattccatt
     3721 atctaactcc gccaagacgt ctatactagt atagaaatct tacgctgagt agggcatgga
     3781 aggcaagacg tgaatgttac ttccctccaa aatcgtcaca ttttttgctg gcaagtccag
     3841 catatcgcgg ataaattgac aaatcattag ttgggctgat acctggatgt ctgagaatcc
     3901 tccttttcct ccttctatta tagcacattt ttaaatctag gtttgctaga ttctatgctt
     3961 ctatctattt attcggaaaa gaaagctaat agcttatttg aaattttctc ctctctgttg
     4021 gatagtcccg tctatctatg ttataatgaa agaaggattt aaaatcggaa aaggagtatt
     4081 tatgctaaaa ttaggtgtca tcggaactgg cgctatcagc catcatttca tagaagcagc
     4141 ccataccagt ggagaatacc aactggtcgc aatctattct agaaaactag aaactgcagc
     4201 aacctttgct tctcgctatc agaatatcca actattcgat caattagaag tcttcttcaa
     4261 atcttctttt gatttagtct atatcgccag tccaaactcc ttacattttg ctcaggcaaa
     4321 agctgccttg tctgctggta aacatgttat tctcgaaaag ccagctgtca ctcaaccaca
     4381 agaatggttt gatttgattc aaacagctga aaaaaataac tgttttatct ttgaagcagc
     4441 tcgtaattac cacgagaaag cctttactac tatcaaaaac tttttagcag ataagcaagt
     4501 gttaggagca gatttcaact atgccaaata ctcttccaag atgcctgact tgttggctgg
     4561 acaaacacca aatgtctttt cagaccgttt tgctggtgga gccctcatgg atttggggat
     4621 ttatcccctc tatgctgctg ttcgtctttt tgaaaaagct aatgacgcga cttaccatgc
     4681 tcaacagctt gacaatagca ttgacctaaa tggagatggt atcctcttct acccagacta
     4741 tcaagttcac atcaaggctg gaaaaaacat cacttccaat cttccttgcg agatttatac
     4801 aacagatgga accttgactc tcaacacgat tgagcatatt cgctcagcta tttttaccga
     4861 ccaccaagga aatcaagtcc agattcctat ccaacaggct cctcatacga tgactgagga
     4921 agtcgctgca tttgcacaca tgatccagca accagatctg aatctctacc aaacttggct
     4981 gtatgatgca ggctctgttc atgagctact atataccatg cgccagactg ctggcattag
     5041 atttgaggca gaaaaatgaa aaccaaacta ccgactgaat ggcaagaact gagtgaccaa
     5101 ctcggtttcc aagaattcac ccccattcaa actcaactat ttgagcccct tcttgctgga
     5161 gaaaacctcc taggagtgag ccaaaccgga actggtaaga ccctagcgta tctcctacca
     5221 agtcttctca gactacaaaa gaaaaaagcc caacaactct tgattctagc accaaacacg
     5281 gaacttgctg gacagatttt tgatgtatgt aaaacgtggg cagaagctat cggattgact
     5341 gctcaactct tcctttcagg ttcaagtcag aaacgacaaa ttgaacgtct taaaaagggg
     5401 ccagaaattc tgattggaac tcctggccgt atctttgaac tcattaaact taaaaaaatc
     5461 aagatgatga atgtggaaac catcatcctg gatgaatttg accaattact agatgattct
     5521 cagattcact ttgtagaaaa aatcactcac tacgcacctc gtgaccacca actggtctac
     5581 atgagtgcca cgaccaagtt tgatcaagaa aagattgtgc cgaatacacg cactattgat
     5641 ctctctgatc aaaaattgga caatatccag catttctaca tgcaggtaga ccaacgtcat
     5701 cgagtggata tactacgaaa actggctcat gtagaggatt tccgtggtct agtcttcttc
     5761 aatagcctgt cagaccttgg gaatgccgag gaaaaactac agtatcgtga tatattggct
     5821 gtttccctcg ctagtgatgt taatgttaaa tttagaaaag tcatcttaga aaagtttaaa
     5881 gataaccaac taaccctgct tcttgcaact gaccttttgg ctcgtggaat tgatatcgat
     5941 agcctagaat gtgtcgtaaa ctttgatatt cctagagata gcgaaaccta cacacaccgt
     6001 gctggccgta ctggccgtat gggcaaggaa ggatatgtta ttactctcgt cactcatcca
     6061 gaagaaatca aaaaacttaa aaagtttgca agtatccgag aaattgtcct aaaaaaccaa
     6121 gaactctata tcaaataaag ccccattgct ctatacacat ccagtgtatg gagctttttt
     6181 ataagcaatt caattttata ctcatctgct ttcaaaaagc attctagtcc atctctgatt
     6241 aacgatggac tttatcacct ccttctccag tccttgtatg acatcttgaa gttgattcat
     6301 gacatcttcc aaagttcgaa aggctttatt cttaaatcca cgtttacgaa tctctttcca
     6361 cacttgttca atggggttca tctctggtgt gtatggagga ataaatgcaa agccaatatt
     6421 agtcggaatc tttaaggtac ttgatttatg ccatatagca ttgtccataa cgagtaaaaa
     6481 ataatcatct ggataagctt gtgaaagctc ttctaaaaag acgttcatcc actcagtatt
     6541 acatccacca gctcttaaga aaaatgattc gcctgtatgg gcatcaacag ctccataaca
     6601 atagcgaaat tctcgtatat agtgactatg gacatgtgga cctactccta ttggagacca
     6661 acaagatccc agtttactga ttctaccgaa accagcctca tcttggtaca tcaagcgaac
     6721 cttatgaaaa ggtctactgg ttttaaagcg ctttcttttc ttcttgaatt gagattttat
     6781 ttttagacgc gacaatggtt tgagcatctg ctttcctagg atgttctgga cgtggcgtaa
     6841 tatttcgcca gccatggcgc ttcaacagtt gatagaaggc atcacgtgtg taggaacgac
     6901 ctaactcctt tttataagcc tgaaataagg catcaattgt aacaaattct cctgcctctg
     6961 cagccttcaa atgacgggca agaaaggctt tctcttcctc aactgtcata tatgcatggt
     7021 tacgaccacc acgtgtttct tgaaggagag agtcgagtcc gaactcctca tattttttta
     7081 cattttgcca aatcgttgtt tgattacagt ctaaaaactc tataatctct ttataagatt
     7141 tgcccatcag a
//
LOCUS       DAVKGC010000099         6841 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKGC010000099 DAVKGC010000000
VERSION     DAVKGC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.099"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            87..3434
                     /locus_tag="VW049_001702"
     CDS             87..3434
                     /locus_tag="VW049_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9432782.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIKE
                     REAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSLP
                     ELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLAG
                     WIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVKE
                     YAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLED
                     ISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRHN
                     WVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDQEGAQSSRMVFSTYPTMIGAI
                     SGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKNT
                     YGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFDS
                     KFEDNSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHDH
                     AEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGID
                     VPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRAD
                     PRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLNE
                     LDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWLL
                     HIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLSD
                     LEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHYL
                     KTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREIIG
                     LDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQL
                     LFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(3939..4409)
                     /locus_tag="VW049_001703"
     CDS             complement(3939..4409)
                     /locus_tag="VW049_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9432783.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4426..6699)
                     /locus_tag="VW049_001704"
     CDS             complement(4426..6699)
                     /locus_tag="VW049_001704"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9432784.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEVELEVKLPHKIELEEPQLNLVQPQGQAELVAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCKTFSLGQEEQ
                     AIQNQYPDKDFERYSKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2100 a         1150 c         1479 g         2112 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatt
      301 ttagggatat cgtagattct gaactccaca agcagatagt tctgttgatt cggtggggaa
      361 accatgctgc tcatggtggt gaaattaagg aacgagaagc gattttagct ttgcatcatt
      421 tgtatcagtt tgttaatttt atcgattatt gttacagcaa tgagtttgtg gagcgttatt
      481 ttgatgagaa gtgcttacca ctttcagcaa acatcaaata ccgagaaact ccacaatcta
      541 tgataaagtt acaagacagt ttaccagaac tgcctgattt tcatgaacag atggctgctc
      601 agtccgtaga agttcaagag acttatactg aaaaacgtga gactgcagcg caacggcaag
      661 atgtgccttt ccatattgat caattatctg aggcagagac aagaaagctc tttattgata
      721 tcgatctccg tttagcagga tggatatttg aagaaaactg tcgtgttgag atagccgttg
      781 atggtctcaa gcacggttca ggaattggtt actgtgacta tgtactttat ggtaaaaatg
      841 ggaaaatttt agcgattgtg gaggctaaaa aagcctctgt caatccagaa gtaggggaag
      901 tacaggtcaa agaatatgct gaagctttgg agaaacatat cggctatcag ccaatttgct
      961 ttattacaaa tgggttgaag cactatatac ttgatggtcc gaaccgccgc cagattgcag
     1021 gcttttactc tcaagaagaa ttgcaattag tgatggatag acgtcatctt caaaaaccgc
     1081 ttgaggatat ttctagtaaa attagggacg atatttccgg gcgtcactac caaaaacatg
     1141 ccattgcaag cgtttgtgaa gctttctcta atcatcgtag acaggcactt ttggttatgg
     1201 caactggggc ggggaaaact cgtacagcag tttctctagt tgatatctta tcacgtcata
     1261 actgggtaaa aaacgttctc ttcttagccg atagaacttc cttggttaag caagcctatg
     1321 attcgtttag aaaattactc ccagatcttt ccgtttgtaa cttcttagaa gatcaagaag
     1381 gagctcaatc aagtcgtatg gtcttttcaa cttatccgac catgattgga gcgattagtg
     1441 gtcaagaaga agtaaatcaa cgccctttta ctgttgggca ttttgacctt atcataattg
     1501 acgaatctca ccgttctatt tatcagaaat acaagtccat ttttgattat tttgatgcaa
     1561 gaattgtagg cttaacagct actccgcgtc aagatttaga taaaaacacc tatggattct
     1621 ttaatttgga gaatggggtt ccaacatatg catatgattt ggaagaggct gttaaagacg
     1681 gatatttagt agcctatcat tctatcgaaa ccaaactgaa actacctacg gatggtctac
     1741 attatgatga tttgtccgaa gaagaaaagg aacattttga tagcaaattt gaagacaata
     1801 gctgtgaaaa agatattgat gggagtgtat ttaattcctt tattttcaat aaaagtacag
     1861 tagaaattgt tttaaatgaa ctcatgacaa gaggaattca gacagcctcg ggtgatgaaa
     1921 ttggtaaaac tattattttt gctaaaaatc atgaccatgc ggaatatatc agaggtattt
     1981 ttaacaaccg ctatcctgaa aaagggagcg actatgctca ggtgattgat tatagtatta
     2041 agcattatca gaccttgatt gatgatttta aaattaagga gaagtatcct caaattgcga
     2101 tttctgtcga tatgttagat acaggtattg atgtaccaga ggttgttaat ttagtcttct
     2161 tcaagaaagt acgctctaaa actaagtttt ggcagatgat tggtcgagga acccgtctat
     2221 gtaaagattt atttggacct gagcaggata aggaaaactt cttggtattt gattatgggg
     2281 acaattttga ttattttcgt gcagatccaa gagatggaga gggtcgtcac attgtttcgc
     2341 tgactcagcg tttatttaat atcaaagtgg acttgattcg agaacttcag ggactccaat
     2401 accaagaaga tcagtttgcg agagcatacc gtcagcagct tgtctcggaa cttcaaggtc
     2461 gtatagagag cttaaatgag ttggacttca gggttcgtat ggttttagat acagtttata
     2521 gctataggaa attggaaagt tggcagaatc taactgctgt tacaagtgaa accattcaaa
     2581 aaaatctctc tccgctttta tttgatgaag ataaagaaga tgagatggcg aggagatttg
     2641 atttgtggtt gcttcatatt cagttggggc aactgacagc taaatcttcc actgttcata
     2701 tttcccaagt gatgaagacg gctagagctc tttctgctat tggcaatatc ccgcaggttt
     2761 ttgagcaggc tgaaattatc aggaaagtac aggagcctga attttggaaa gaagttaact
     2821 tgtctgattt ggaaaaaatt cgtcttgcta ttcgagattt attacagttt ttggataaaa
     2881 cagaccgtaa accctactat gttaactttg aagatcgtat actctccact gttcacgaga
     2941 ccacagcatt tttgcaggtc aacgatcttc ggtcttacaa tgaaaaagtt gagcattatt
     3001 tgaaaactca tctggatgag gagtccattt ctaagctata ccataataaa aagttgacat
     3061 ctgatgatat gcttgcactt gaaaaattgc tctgggaaaa attaggtagt aaagcagact
     3121 accaaagtca ttatgaaaat aaggcaattc cgagattggt tcgtgagatt attggcttag
     3181 atagagagtc tgccaatcgt attttttcta aatttttgtc ggatgagaat cttaatgcca
     3241 ggcagatttc atttgtaaaa ttgattgtag actacattgt agaaaatggt tttttagaga
     3301 cgaaagtgtt aacgcaagag ccgtttaaat cttatggttc tgttcaacta ctcttccaac
     3361 accaactacc agtacttcgt aatattgttc aaatcattga acttatcaat aatcgagctg
     3421 gagaagcggc ttaaattcta aagtgattgc catgctgaga ctcatttaaa attaaaaaga
     3481 gtagaaattt atgctatata tgagaagttt tattaggaag aatgtcatcg ttttcctaga
     3541 atacagtatc agttgttaag tggttgataa atttcaaagt agatacttgt accacgatgt
     3601 ttgttgatcg agttattaac aaaagagcta ctttgatttt aaagaaatag aaaacaaaaa
     3661 gctgagcaag aattcaattg caggagaaaa tgaaataata ctcaatgaaa atcaaagagc
     3721 aaactaggaa actagctgca ggctgctcaa aacactgttt tgaggttgca gatggaagct
     3781 gacgcggatt gaagagattt tcgaagagta taaatcttcc taggataaag caaaacgcat
     3841 agtatcaagg gttttcaaca cttgatacta tgcgttttct gatgttaaag actttctacc
     3901 agtttttttt aagcataatt gttagttgta gtcatttatt attcttcaaa gaaaaatggt
     3961 ggggcgaatt ttttcagttc ttcaaagcac ttttgagcag tatctgcatc ttcacagatg
     4021 ataagacaga catcattacc acaaagggta gcgatagcgt cagggaagct caaagtatca
     4081 atgatagaac caaaggattg agccagtcca ggaagggttt ttagtaggac ttggtgttga
     4141 actgggcgca tccagacaag ggcgtcttcc atgtagagtt cgagacgttt ttcccatttt
     4201 gagatggaac cattgttaag aacataataa gcgctatctt cttcgcggac ttttgatagg
     4261 ttcatatttt tgatgtcgcg tgagagggtt gcctgggtta cttgaatgtc gttcttagca
     4321 agaagggctt gcaactcagc ctgtgtatga atcttgtttt ttgtgataag agcgcgtata
     4381 agttggtggc ggtgttctga tttattcata ataatgtaac tccttttagc aaggtaaggt
     4441 aagcatggac tgagcgaggt cgacagtcaa gtggtagtct gtattgtcac ggatggtgat
     4501 ttcaaagtca gtagtataga ggactaaacg gagagtgtct ccttctttta gcttgtaaat
     4561 agttggctgc agttcaaatt gaacatccat ccattcatct gcagtaatat cctctactaa
     4621 cagtaaatca tttctatttt gtaaattaag gtaacctttt gtcacgactc gttgtgcctc
     4681 tggtctaaat ggcaattcac agagattttc caacatgtga tagcgaccgt tgtcaatggt
     4741 tctagcactt aaaatagctg gataaggttg taggtatttc ttttgcccaa attctagcag
     4801 ttgggcagat aagagcccct tgtttgtact ggatttgata cgaagattga gctgagcgcg
     4861 accgtttagg tggagatctt tagtcacagg aaggttaata gtaatctgat tagctttccc
     4921 ttgatagagc tctgtattga aggtttggta tgtcttacta tagcgctcaa aatccttatc
     4981 tgggtactgg ttttgaatag cttgctcttc ttgaccaagt gagaaggttt tacagttttc
     5041 ttgcccaccg aagttatcaa gtgataacca agtctgtgga gctgtattgt cctgccagat
     5101 aacagtagga agttgaaagt ctgtttcctg tcctagtaat ttcttggtta ataaggcatt
     5161 tatggactca cggaagtcaa ttgattgcca attgttcatg taaacatggg caccattatg
     5221 gaaaaagaga tgcttgtgta tatgagtagg aagagcatgg aacatctggt aaacatgaag
     5281 tggtttgaca ttccaatcct gagaaccatg agtaaagaca acctctgcct ttactttatg
     5341 ggcattgagc agataattgc ggtcatgcca aaactgattg tagtccccag tttttcggtc
     5401 tagctgagct ttcacttttt ctaagtcagc ttggtgagct tcattgccac ggatatagtc
     5461 gccagctaag agattacgag aataggttaa ctcagcaagg gagtcaaagt cctcacctgg
     5521 ataaccacct gggctagtca ccagaccgtt ttcacggtag tagttgtacc atgatgaaat
     5581 tcctgcctcg gcaatgataa cttctaaacc atcgactcct gtagtcgcaa gaccattgga
     5641 catggtacct agataggaaa gtcccgttgt agcaactttt ccgtttgacc aatcagcctt
     5701 gacttgacgc tggcgcgtgt gatcagtaaa ggcacggcaa cggccgttaa gccaatcgat
     5761 gacattttta taagcctcga tttgctggta gtctccatta gtcatgaaac ctgtcgagtc
     5821 tttggtacca acacctgaga catagagatt ggcaaagcct ctcggaagga agtagtcgtt
     5881 tagtgtatag ctagagttga tgtgagttag cttttcctca gcctctgcta caagctcagc
     5941 ttgaccttgg ggttggacga gatttagttg aggttcctct agctcaatct tgtgaggaag
     6001 cttaacctca agctcaacct ccatcttgta gagagccttg tcactagcct tgtcattggt
     6061 tccctgatga taagggctgg ctgtcatgat ggcagggatt tttccatcaa aacgagggcg
     6121 aataatgcta acctttacta ggtctgatag ccctttttgg tcagtatcga cacgagactc
     6181 aacgtaaacg acttcacgaa tgacatcctg gttagaaaaa gtagccaaac tcttgccgtt
     6241 aaagtagtgg tagtcattat cctccggaat aagaccatca ctaacaagtt ggtcgataag
     6301 agtatttcct tttttggtgc gagtattgag taactgatag agattttcaa tcaagtcacc
     6361 atatataatg ggaaatccag tttctttacg aaaaacgtca ctatcttcga agtcaaccaa
     6421 ataagaaaag cctaaaagtt gaaaagcaac agtataaaaa atatctgctg tcagttcatc
     6481 ttctgattga aaaaatgtca gcaggtctgt ttttttatca gctgctagga tagaaagtgg
     6541 gtagttggtg tcttgataag tgaaaaagaa acgacgtaaa aaggtttcaa gtgagtcttt
     6601 gtgattggct gtattttgta aatcaaagcc acattttttt agctcagata agacattttc
     6661 ttttggaaaa ttgatataac tatattgatt aaaacgcata gaacctccat atagaatgac
     6721 agttaaggtt attatatcaa aaaaaaagca gaaagggaat tgttaacttc aaaaggaaat
     6781 aatccaataa aaatgaataa agtactaaat tcaatataga gaacagagta acaataagaa
     6841 t
//
LOCUS       DAVKGC010000100         6722 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKGC010000100 DAVKGC010000000
VERSION     DAVKGC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.100"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(61..474)
                     /locus_tag="VW049_001705"
                     /pseudo
     CDS             complement(61..474)
                     /locus_tag="VW049_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(550..984)
                     /locus_tag="VW049_001706"
     CDS             complement(550..984)
                     /locus_tag="VW049_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9432785.1"
                     /translation="MLLVLVSLAHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYKLLREKLLEQPLLPADETSYRVLESDSHLTYYWTF
                     LSGKAENQAITLYHHGQCRSGLVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(1060..1911)
                     /gene="rfbD"
                     /locus_tag="VW049_001707"
     CDS             complement(1060..1911)
                     /gene="rfbD"
                     /locus_tag="VW049_001707"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU9432786.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            complement(1977..3026)
                     /gene="rfbB"
                     /locus_tag="VW049_001708"
     CDS             complement(1977..3026)
                     /gene="rfbB"
                     /locus_tag="VW049_001708"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU9432787.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(3036..3632)
                     /locus_tag="VW049_001709"
     CDS             complement(3036..3632)
                     /locus_tag="VW049_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU9432788.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(3633..4502)
                     /gene="rfbA"
                     /locus_tag="VW049_001710"
     CDS             complement(3633..4502)
                     /gene="rfbA"
                     /locus_tag="VW049_001710"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU9432789.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGLKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            complement(4659..5051)
                     /gene="tagD"
                     /locus_tag="VW049_001711"
     CDS             complement(4659..5051)
                     /gene="tagD"
                     /locus_tag="VW049_001711"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HEU9432790.1"
                     /translation="MKRVITYGTFDLLHHGHINLLRRAKELGDYLVVVVSSDEFNLIE
                     KNKVCYFNYEHRKSLVEAIRYVDLVIPETSWEQKRSDVKEYHIDTFVMGDDWIGEFDY
                     LKEEGVEVVYLPRTKEISTTKIKKDLSM"
     gene            complement(5079..5894)
                     /locus_tag="VW049_001712"
     CDS             complement(5079..5894)
                     /locus_tag="VW049_001712"
                     /inference="COORDINATES: protein motif:HMM:NF016852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU9432791.1"
                     /translation="MTETNKQLHETLIEILDFVKEICEKHELTYFLVFGTALGAKRHC
                     GFIPWDDDVDIALPREHYNIFIDALSETDQSIFSLQNEDNEPNYFLPFAKLRKNNTIF
                     IEKILDVEYENNGIYIDIFPLDFVENPDSFNFKIRRTTFNYIKHILKFSSCRSFYKNK
                     YSNVRYLIENIMSIPTLFFSNRRLLFLANSLISSTTKADFIGQYDQKSNKRAIMPSNY
                     YFPPRSAVFEGKTYSVPAKLEDYLKCFYGSDYMELPPIEKRVTHQPITLRFEK"
     gene            complement(5904..>6722)
                     /locus_tag="VW049_001713"
     CDS             complement(5904..>6722)
                     /locus_tag="VW049_001713"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014051.1,HMM:NF026018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEU9432792.1"
                     /translation="HFFCKKYWLYALNFNLPLIPHYLSQSVLNQSDRIMINSMVGMGE
                     AAIYSVAYSISILMLLVTSAINSSFIPYTYKCIRDKKYTELGKSANLLITLVGIGSIL
                     TISLGPEIIQLFAPKQYYEAIWIIPPVALSVYFMFLYPIFGNIEFYFEANHFVMWASI
                     GGAIMNIFLNFIFLKHFGYIAAGYTTLFCYILFALGHYIFMRRVLYQNLSGIQIYNSR
                     YLLFFSILLIILMLLIVIIYPFILIRYLTISLILGGCFLKRKKIMSSIAIIKQQ"
BASE COUNT         2051 a         1432 c         1113 g         2126 t
ORIGIN      
        1 aaccaccctc gcttttttaa actgagctta ggctaaaaga gtccactgga ctcttttact
       61 ccgtcccata accaatgatt taatggagca ttcagctaga ttattggaaa ggaccagatg
      121 tccgtctttc aaaatagtct taaaggtttc ttcatacttg aggctgtatt caattgccct
      181 tcctagtttt gaacctgcta aaactgactg acggcggcac caagcaaaga agtcttcctt
      241 taagggttgg agctcttctt gacgtttctg tagtcgttca tcagctggca aagcctccca
      301 gtctctttcc aaggaaaata actgattaca ataagctaaa cctttagctc ctaaggatga
      361 tttatccgct tgcttggggg tcgcttcaaa aaacttcctt ctcacatgtg cccaacatcc
      421 aacaagtgca gcttcttcca gttgacgata agcttctagc ggttcgcagt ttaccaagct
      481 tagcgtcgcc ttgctcctaa accttggact gctatcgcat aggcagaact agaggactaa
      541 agtcctaagt tactgccgca acatatcaca atgcacatag ccagaataat ctcctaggaa
      601 ttcttgtact actaaaccac ttcgacactg accatgatgg tacagcgtga ttgcttgatt
      661 ctcagctttc ccagacaaaa aggtccaata gtaggtcaga tggctatcac tctctagaac
      721 ccgataagag gtttcatccg caggaagaag aggttgttct aacaactttt ctcgtaaaag
      781 cttataaagg gactctaaat agtattgact tgccttgata tgccaattag agatttcctt
      841 acgtgtgatt ggtaaaccca tcctagccca atcttcttct tggcgataat tgggtacctt
      901 cagattaaac ttctgatgga tggtgtgagc gataatagaa actgagccaa agctatgcgc
      961 caaagagacc agaaccaaca acaagataag cgtacatctt aagctccttt ggagttcatt
     1021 atctgcctct tttattagac tagaaaatca ttctactact tatctcactt cttgtttgta
     1081 aaattcttgc aatgcatctt gccaagttgg aataacaaat ccagtagctt tggctttggc
     1141 caggctcatc gttgagttta gcggacgttt agctttggct ggaaattgac tggaatctac
     1201 tggcttgact tcgacatctg tatctttcaa aatttcaact gcaaaatcat accatgttgt
     1261 gtcttctgtc gcatcatttg acaaatgata ataaccaaat tccttacgat tttcagctag
     1321 gtaggtcatg aactcagcca aggtacgagt ccaagtcgga cgaccgtact ggtcatttac
     1381 aactgttaaa gtcttatgag ttttcgcaag attttgcatg gtaaaaacga agtttttgcc
     1441 ataatttcca aatacccagg cagtacggat aatatagaaa ttagacacat gcttctcaac
     1501 taactcttcc cccatacgct tagtgcgtcc atattctgtc tgtggatctg gtcggtcatc
     1561 aacttcccac tcttgtccaa ctggtttctt accgtcaaag acatagtccg tagaaatata
     1621 aactagagtt gcaccatgct tttcagatgc ttttgcgaca ttttttgtcc ccgtcacatt
     1681 gatggcgaag tccaactctt ttccttcatc ctctgctgca tcaacagcgg tgtaggctgc
     1741 acaatggtag actaaagtcg gtttcacctc ttcaaaaact ttctcaacca tttcttcatt
     1801 ggtaatgtcc atctcagcca catctactgc cacgtattct tcattacgtt catccaataa
     1861 atagcgaagt tccgttccta attggccatt tgcccctgta attaaaatca tctctagacc
     1921 tttctaatat gtaactcttc ccaatatagc aataaaaagc agctatttcc tgctttttat
     1981 actgtaataa tctcctgagt cttagcataa ttggcttcaa cagcttcttt ttctgctttc
     2041 caccattctt ggttatctgt ataccacttg attgttgcct tgagcccagc ttcaaagttg
     2101 gtaaattcag gtttccaccc caactcatca cggagcttgc tggcatcaat cgcatagcga
     2161 aggtcatgtc ctgcacggtc agtcacatga tcataggcat ccgtagcttg tcccatttcc
     2221 ttaaggataa gttccaaaac ttccttattg ttcttctcac catcagcccc aatcaagtag
     2281 gtttcaccga tttgcccttt tgtcaagatt gtccaaactc ctgaagaatg gtcattggta
     2341 tgaatccagt cacgaacatt cttaccttca ccgtaaagtt ttggcttaat tccacttagg
     2401 atgttagtaa tctgacgtgg gatgaatttt tcgatatgtt gataaggacc gtagttattt
     2461 gaacagttgg aaatcgttgc cttgactcca aaagaacgca cccaggcttt gacaatcaaa
     2521 tctgaggctg ccttggttga tgagtacgga gagcttgggt tgtattttgt ttcagcagta
     2581 aatttctcac ctggtccttc accatgacct ggcaaatctt cgcgtaaagg gagatcccca
     2641 taaacttcat ctgtcgatac atggtggaag cgaatatcat acttacgagc agcttctaaa
     2701 agagtatagg ttccgatgaa gttagtatga ataaatggcg atggatcatt gagcgaatta
     2761 tcattgtggc tttccgctgc ataatgaacg atagcatctg cttgagcagc caacttgtct
     2821 accaactccg catcagcaat gtcaccaaca actaactcaa cacgattacc taaaatttcc
     2881 tcaatattcg cgcggtttcc agcataagtc aacttatcta ggactgtcac atgaacatct
     2941 ggaaagttct cgtaaacata atggacaaag ttagaaccga taaagccagc tccacctgtc
     3001 acgataatat ttttgtattc agtcatattc tttccttatt ccaaatcttc ttttttcaaa
     3061 ggttttacat ccttaagtag tggatgatgt ttgtctgctt ctgaaacctc tgcttctgca
     3121 atattttccc attcaatacc aaggcttgga tcagcgtagt tcacaaaggc atacttgggt
     3181 ttgagttcaa gagcccagta atcattgacc agatagctat atgacactgt atctgataaa
     3241 acttggaagc cattagctac gcctcgagga acaaagattc ccttactcgc atcaatcact
     3301 gtctgatagg tattcccaaa ggtttcaccc tcgcgtagat caacccaaga acccagaact
     3361 ttccctccat ctgctacaga gatgtactta tcccaaggct ctgcgtggag acctcgaagg
     3421 acatttttac gtgagaagga tacattgttt tgcaattttc cttctgcaaa gaaagactct
     3481 ggaaatccaa gtggaagcat tttttccttt tggaaatttt ctttaaacca gccacgatta
     3541 tctccatgaa cggggatatc aaactccaac atgcctggaa tagcttcaac cttgcgtgcc
     3601 gcaagcgtct taccgaaaaa attatctgtc atctatgctt ctccaatcaa acggagcaga
     3661 tactgtccgt attcattttt cttaagtggt tgggctaagg ccaatacatc ttctcgactg
     3721 atatagccca tacggtaagc aatttcttct aagtttgcta cctgaacatt ttgcatccgt
     3781 tgcactgttt cgatgtactg tgaagcctct agtaaacttt catgagttcc agtatccagc
     3841 caagcaaagc cacgtcccat aagctcaaca gacaaatcac cacgatctag gtaagccttg
     3901 tttacatctg taatttccag ttcaccacga ggacttggtt taatactctt ggcaatctct
     3961 actacatcat tatcatagaa atagagacct gtaactgcat agtttgaacg aggttgctct
     4021 ggcttttctt caatagaaat agccttcata tcctgatcaa actcaaccac accaaagcgc
     4081 tctggatcct tgacatggta gccaaaaaca gttgctcccg actccttact cgctgccttt
     4141 tgtagcatct tggaaagccc agaaccgtga tagatattgt cacctaagat cagcgcaacg
     4201 ctatcatcag aaataaactc ttccccaatg ataaaggctt gtgccaaacc atctggactt
     4261 ggttgctctg cataagaaag tttgagccca aactcagatc cgtcttgaag aagctcttgg
     4321 aatcgatgta aatcctgtgg agtggaaata atcaaaatat ccctaatccc agccaacatc
     4381 aatgttgaaa gtgggtagta aatcatcggt ttatcataaa ccggcataag ttgttttgat
     4441 gcagcgcgag tcaaaggata taaacgtgtc cccgaaccac ctgctagaat aatacctttc
     4501 atagtagggt acctttctta tttatctaat tacgatattt tttacctata ttggtagtgt
     4561 cctatcattt ttctcttttc tatcgatttt agcatttcta cttaaaaata cagtggtcta
     4621 ttcgagaaca ataacagaac aatgcttatt tacgaatatt acattgataa atcttttttt
     4681 attttagttg tcgaaatttc tttcgtccga ggtaagtaaa cgacttcaac tccttcttct
     4741 tttagataat caaattctcc aatccaatca tcccccatca caaaagtatc aatatgatac
     4801 tctttgacat cactgcgttt ttgttcccaa ctcgtttcag gaataacaag gtcaacatat
     4861 ctaatagctt caactagact ttttctatgc tcataattga aataacaaac tttatttttt
     4921 tcaattaggt tgaattcatc acttgaaaca actaccacta gatagtcacc taattctttt
     4981 gcacgtcgca ataaattgat atgtccatga tgaagtaaat caaacgttcc atatgtaatt
     5041 actcttttca ttatattctc cttctacaga atagcaaatt atttttcgaa tctaagtgtt
     5101 attggttgat gagtgactct tttttctatt ggtggtaatt ccatataatc cgagccataa
     5161 aaacatttta aataatcttc taatttcgca ggaacactat aggtctttcc ttcaaacaca
     5221 gcggacctag gtggaaaata atagtttgaa ggcataatag ctcttttgtt acttttctga
     5281 tcatattgac caataaaatc agctttagtc gttgatgaaa ttaaactatt cgctaagaat
     5341 aataatctcc tatttgaaaa aaacagggta ggaatactca taatattttc aatcaaataa
     5401 cgcacattac tgtatttatt tttataaaaa cttctgcaac ttgaaaattt taaaatatgc
     5461 tttatataat taaatgtagt tctcctaatc ttaaaattga aagagtccgg attctctacg
     5521 aaatctagtg gaaaaatatc aatgtaaatg ccgttattct catattcaac atcaagaatc
     5581 ttctcaataa aaatcgtatt gttttttctt aatttagcaa atggtaaaaa ataattcggc
     5641 tcgttatctt cattttgaag actaaatata gattgatccg tctcagataa tgcatcgata
     5701 aatatattgt agtgctctcg tggcaaagca atatcaacat catcatccca tggaataaat
     5761 ccacaatggc gttttgctcc aagagctgtc ccaaagacca aaaaataagt taattcatgt
     5821 ttttcacaaa tttcttttac aaagtctaaa atttcgatta atgtttcatg taactgttta
     5881 tttgtttctg tcattcttgt acctcactgt tgtttaatga tagctatcga actcattatc
     5941 tttttcctct ttaaaaaaca tcctcctagg atgagtgaga tagtaagata acgaatcagt
     6001 ataaatggat aaataatcac gattagtagc attagaataa taagaagaat cgaaaaaaat
     6061 aaaagatatc tggaattata aatttggata cccgaaagat tctgatacag tactctcctc
     6121 ataaaaatgt aatgtcccaa cgcaaataaa atataacaga ataaagttgt atagcctgcc
     6181 gcaatatatc caaagtgttt taaaaaaata aaattcaaaa aaatattcat aattgctcca
     6241 ccgattgaag cccacattac aaaatgattt gcttcaaagt aaaactcaat gttaccaaaa
     6301 atgggatata agaacatgaa atatactgac agagcaacag gaggaataat ccatattgct
     6361 tcgtaatatt gttttggtgc aaataattga atgatttctg gtccaagaga aatagttaaa
     6421 atcgaaccaa tgcctaccag agtaattaaa agatttgcac ttttccctag ttcagtatat
     6481 ttcttatctc gaatacactt atatgtgtaa ggaataaaag agctgtttat agcacttgtc
     6541 actaaaagca tcaaaattga tatgctgtaa gcaacactat atattgctgc ttctcccatt
     6601 cctaccattg aattaatcat tattctatcc gattggttta aaactgattg cgataaataa
     6661 tgaggaataa gaggtaaatt aaaattcaaa gcatagagcc aatatttttt acaaaaaaaa
     6721 tg
//
LOCUS       DAVKGC010000101         6623 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKGC010000101 DAVKGC010000000
VERSION     DAVKGC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.101"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     regulatory      74..172
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            256..1047
                     /gene="thiD"
                     /locus_tag="VW049_001714"
     CDS             256..1047
                     /gene="thiD"
                     /locus_tag="VW049_001714"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9432793.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGVAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(1257..1889)
                     /locus_tag="VW049_001715"
     CDS             complement(1257..1889)
                     /locus_tag="VW049_001715"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9432794.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(1882..2685)
                     /locus_tag="VW049_001716"
     CDS             complement(1882..2685)
                     /locus_tag="VW049_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9432795.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(2685..3209)
                     /gene="thiW"
                     /locus_tag="VW049_001717"
     CDS             complement(2685..3209)
                     /gene="thiW"
                     /locus_tag="VW049_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9432796.1"
                     /translation="MRKHQLQVHKLTILSMMTALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(3214..3906)
                     /gene="tenA"
                     /locus_tag="VW049_001718"
     CDS             complement(3214..3906)
                     /gene="tenA"
                     /locus_tag="VW049_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9432797.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(3917..4567)
                     /locus_tag="VW049_001719"
     CDS             complement(3917..4567)
                     /locus_tag="VW049_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9432798.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(4569..5954)
                     /locus_tag="VW049_001720"
     CDS             complement(4569..5954)
                     /locus_tag="VW049_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432799.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(5955..6515)
                     /locus_tag="VW049_001721"
     CDS             complement(5955..6515)
                     /locus_tag="VW049_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9432800.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         2056 a         1442 c         1314 g         1811 t
ORIGIN      
        1 catttgaaaa acgatttgca gtcaaatatt actatataaa caataaaaat atgctatact
       61 aaagaaaaaa gaaaacaacc actaggggtg cgtaaagctg agattaacga ctgttagatc
      121 cctctgactc aatctaggta atgctagctg atggaagtgg aaatgataat ggggactagc
      181 agtcttctat tgcctttcta aaacagacta gcttgttctt aagaatacaa acttcagttg
      241 gttgggaggt tttagatgac ttatttaccc gttgctttga ccattgcagg gactgaccct
      301 agtggtggtg ctggcattat ggcagattta aagtcattcc aagcgagaga tgtctatgga
      361 gtggctgttg taaccagtct tgtcgctcaa aataccagag gtgttcagct aatcgagcac
      421 gtttctcctc aaatgttgaa agcccaattg gagagtgtct tttctgatat tccacctcag
      481 gctgtaaaaa ctggaatgtt ggctactact gaaatcatgg aaatcatcca accctatctt
      541 aaaaaactgg attgtcccta tgtccttgat cctgttatgg ttgctacaag tggagatgcc
      601 ttgattgact caagtgctag agactatctc aaaacaaact tactacctct agcaactatt
      661 attacgccaa atcttcctga agcagaagag attgttggtt tttcaatcca tgaccccgaa
      721 gacatgcagc gtgctggtcg cctgatttta aaagaatttg gtcctcagtc tgtggttatc
      781 aaaggcggac atctcaaagg tggtgctaaa gatttcctct ttaccaagaa tgaacaattt
      841 gtctgggaaa gcccacgaat tcaaacctgt cacacccatg gtactggatg tacctttgct
      901 gcagtgatta ctgctgaact agccaagggc aagagtcttt accaggcagt tgataaggcc
      961 aaggccttta tcacaaaagc tattcaagat gcccctcaac tcggtcatgg ttctggtcca
     1021 gtcaaccata caacttttaa agattaagaa aaaaaactct ctagttccca ctttaaggga
     1081 attagagagt ttttatactc ttcgaaaatc tcttcaaact acgtcagctt ccatctgcaa
     1141 cttcaaaaca ctgttttgag ctgacttcgt cagtcttatc taaaacctca aggcagtact
     1201 ttgagcaacc tgcgactagc tttctagttt actctttgat tttcattgag tattaattag
     1261 gaaagaatgt catgcaactt tgttaaaaag gcttgcgttt ttgcctcaat atcttctgct
     1321 tgcatcaaat cacgtacaac agctacacca gctatgccag tgcccataag ctgatcaata
     1381 ttctccgaag tcaagccacc aatagcaact attggaatgg caaccgtttg gcaaattgtt
     1441 ttcaaggttg aaatcagggt gatgggtgca ttttccttgg tggttgtcgg aaaaatggct
     1501 cctgtcccca agtaatctgc acctgatttc tccgcttcca gagctctttt aaccgtttta
     1561 gcggtgacac cgaggatttt ttcaggaccc aagacttgtc gggcaaccga aactgggagt
     1621 tcgtcatctc caatatgcag accagcagca tcaaccgcaa gacaaacatc caaccgatca
     1681 tcgattatca agggtacctg ataagcatct gttatttcct tgacttgttt tgccagttga
     1741 taatattgat tggttgtgag atttttttct cgcaattgga ctatggtaac ccctgaacgg
     1801 caggccgtct caacttttgc aagaaagctt tccacggaat cttgatagcg attggttacc
     1861 agatatagtc taagtgcttc tctattcata aacctctcct ttgatggtat ctagccaatt
     1921 ttcatctctt cttaggagcg aaagctgatt gagtacttgg taacgaaatt cttccaatcc
     1981 cattccttga acaactattt tctcagcagc gatattgaga taagagactg ctaagcaaga
     2041 agcttcaaaa ccagtctttc cttggctgag aaaaacggct gttaaggctc caaccaaatc
     2101 tcctgtccct gttatccagt ctaattctgc acagccattt cccagtacag cgacctgatt
     2161 tttcgaaacg acgaggtcct tgggacctgt gactaagaaa gacataccat gataggtctg
     2221 acaccagtct ttcaagactt gaagcaaatc ctccgtttct tgatctttag cactcgcatc
     2281 gaccccaacg ccgtggtgct ttaatccaac aagacttcga atttctgaca tgtttccttt
     2341 aaggaccgta ggtctatagt ctaaaaggtc tctgactaag ctcttacgaa tggatgaagc
     2401 tgttacgcca accgcatcta ctaccattgg gagacaaact tgagctgcat aagaagctgc
     2461 tatacggatt gctttctcct tctcagctga caaatgcccc aaattgatga agagagcctg
     2521 actttgctta gtaaaatcaa gaacctcacg gggatcatct gccatgacag gtttgcatcc
     2581 cagagccaaa atcccatttg ccagcatctc acaagaaatc tcattggtca tacagtgaat
     2641 gagggaacta gagcctatag gaaagggatt tgtaaattcc tgcatcagtc tatcctttca
     2701 ctaaagaaat atccctgcac ttttttaaag aattcctgct tgattaaaaa tcgaaaggca
     2761 ataaaggaaa tcgctgtacc aatcaaggtt gctccgaaaa atcgaggcgt gtagataaac
     2821 cagctaagct tagcagctga tcctgtaaag agtaccataa caggatagga aacaatagaa
     2881 ccaataatac ctgttcccac aatttctccc aaggcagaaa agtaaaattt tcgaccgtac
     2941 ttataaaaga gacctgctag aagggctcca aaagtcgctc ctgtgagagc taaaggcgga
     3001 atcccttgag tcgtcatacg gataaaggct gtcactgtag ccatggccaa ggcataaaca
     3061 ggtcccatca tgattcccgc tagaatattg actacactgg acatcggtgc cattccctca
     3121 atccgaaaga taggtgtaag gactacatca agggcagtca tcatagataa aatggtcaat
     3181 ttgtgaactt gtagttggtg ctttctcatg tttctattct tctccttttt ctaaagactg
     3241 taaatcgctc ttccatgtct ggtgttggta agccatctcc caaaacttgg cttccatatg
     3301 aacactgatg tggaaggcat ctagcatttt ttgcttatct gtctcatcac tttctcgata
     3361 gagctgattg accagtgctc cctcctctct gatctgttgc tctaactcat ccgtaatata
     3421 agtttcaatc cattgttgat agagaggatt tggtgatggt ttaagattaa gtgatttgcc
     3481 tatatcatgg tataaccaag gacaaggaag caagcttgca aaagctatgg ctaagttcgg
     3541 ttctgcaaat tgcctataaa tatgagaaat gtaatgataa caggttggag cgattggatg
     3601 ttgctccatt tcctggtcgt tgatttccaa ttccttgaaa aattgttggc gaataaataa
     3661 ctcaccctcc actaaaccct gagcattttg tttcaagagt cttttcatct cttggtttga
     3721 agtcttatca gccaaaagat gatagatttc tgagaaagcc ttcagatagt aggcatcctg
     3781 aatcaggtaa tagcggaaaa tggcaggttc taaattcccc tcttgtaatt gtaaaataaa
     3841 gggatgatga aaggaagcct gccaagcttt cttggataat tccatcgcaa tatctgtaaa
     3901 ttccataata actcctttat aaaaatagac tggtttgaag caataaaaag aaaagcaggt
     3961 agattaattt tgttttttta ggaatataaa aagtccgata gctattcttc aactgtgcat
     4021 gttcgtcata tccgtgagca gatagagctc tcaggtaaag atggcgccac ctaaagactg
     4081 tcatcagaac cttactgtaa atcaagggcg accaaaaatg tagtttttga ccacgtaata
     4141 ggcaagcttc tttgagggac ttgatttctt gctgaatgag aggaaaagaa ttgaatacca
     4201 caatcaaggc ataggaccaa gagcgtgata gccccttttg agccaagtac aagagaagct
     4261 cttttagtga aatagaggaa acaaagacaa ggccgataca aactgtcaca aaggccctcg
     4321 ttccaagcat gactgcctgt gaagcatctc cgtgtaactg aactgcccag tagttggcaa
     4381 aagatggtaa aatggcaagt atgatcatcc aagctaacat tttaaatcga cggtaataga
     4441 gcataaagag aatacaaaat gcgactaccg aaagattcag agcaatcgaa ggaatgaaag
     4501 atgtttccaa ggataaaatc agcaagaaga gactgataat cggtgtctgg gttgctactt
     4561 tgaccatact atctcacctc cccttgggta ttgctactct gagatgtaag tggtttggta
     4621 atggtcactt ctttcacatg ccgaagaccc tgactagtca tctcaatcca ataatcaacc
     4681 acagaaatca aagggtctaa acgatgacta atgagcagaa aacttcttcc ttgattcctc
     4741 tcctccacaa tccacttgca aaaataatgg caggctctat catccaaacc tgcaaaaggt
     4801 tcatctagca agatcacgga agccttactg gtcaagatgg tcaggagctg aagaattttt
     4861 tgctgaccac cacttaattg atagggactc ttatcgactg cctgctccaa atcaaaatat
     4921 cgtaaagctt gaaaaatccg ctgatttctt tcagaatcag gtccatctaa ttgaagctcc
     4981 tctcgcagac tgactcggat aaactgcttc tcagcttcct gaacaacacc agtcagatca
     5041 cgatacaaac tctttttctt tttcaggacc gaacccttcc atgcaatgcg ccccttatac
     5101 ttttgaaatt gaagaataga ccgaaagagg gttgatttcc cgacaccatt gtcacccagg
     5161 atacaggaaa tcccttgata gaatgtgaaa tcagcaattg aaaagagggg gcgatttcca
     5221 aactcacaag tcgttctatc catatggaat agttccgggc tagaagcaac ttcctttgaa
     5281 gcaacctgtg tcatctcata ggaagggatt tgaaacactt cccttagttt tccgtctctt
     5341 agctcaacca tatggtcgat ataggcttta tagtcagata aatcatggtc gcacaaaata
     5401 actgtctttc catcaagagc caactctttt agaatctcca atatctcgat tctgctcttg
     5461 cggtcaatgg aagcaaaagg ttcatccaag agatagaccc taggattcat ggcaaagagg
     5521 acagccagcg ctgctttttg cttttcccca cctgataggt gatgaatggg acggtgtaag
     5581 attgcctcgc aacgacattg ctggacaacc tctgctattt tagaatcaat ttcctgaagg
     5641 tgatagccga tattctccaa ggtgaaaatc agctcctcaa acaagctctc catggtaaat
     5701 tgatgattgg gattttgaaa gagaatgcca accttctgga cacgtgcgat gatagaaagt
     5761 tgactgacct cgctcccatc tatcaggact tgaccactat agggaagaga actgacttga
     5821 gcaatgactt gaaaaaggct ggattttcct gaaccactgc tcccaactaa caaggtaaag
     5881 gcttgcgcat gaaaagtaaa atcaaacggc tcagagaaga ttggggactg aatcgctcgt
     5941 agttccagac ccatctatgc ttttcctcca gttgcaaact gatgatagag tttgacaatg
     6001 gcacgaacca agatggtaca gaagaaataa acagaaataa aacgtaccac aagtaaggaa
     6061 aggacaaacg gaagggaaaa ggcgtagtaa cctaacttaa tgtattcata gacaaagcta
     6121 acaagcgtaa tcccaatact attagcagtt agagagagcc aactttcata gcgattctta
     6181 gttacgataa aaccaaattc acttcccaaa ccttgaacca agccagacaa aagagctcct
     6241 agaccaaatt ggctaccata aaggacttca gcaagcgcag ctagcacttc tccaatcgtt
     6301 gcacttccga ctctcggaac aaagatggca gcaatgggcg cagccataca ccagagaccg
     6361 aagaggattt cattggcaaa ggcctgcaaa ccaagaggtg ttaagagtag actgagaata
     6421 ttatacacat atcctgaacc aacgaaaacc ccaccaaaaa agatagacaa gaaagcaagc
     6481 aagataacat cttttaactg ccattttttc aacataaaaa actccttttt ttaaagaaaa
     6541 gtggggcact caagaagacc gacctaaata ctttgtatag cagactgaat ttagaacagt
     6601 acacaagaat gctaaaatat ttc
//
LOCUS       DAVKGC010000102         6621 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKGC010000102 DAVKGC010000000
VERSION     DAVKGC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.102"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            37..2016
                     /locus_tag="VW049_001722"
     CDS             37..2016
                     /locus_tag="VW049_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9432801.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            2083..3579
                     /locus_tag="VW049_001723"
     CDS             2083..3579
                     /locus_tag="VW049_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9432802.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            3579..4505
                     /locus_tag="VW049_001724"
     CDS             3579..4505
                     /locus_tag="VW049_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9432803.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            4514..5581
                     /locus_tag="VW049_001725"
     CDS             4514..5581
                     /locus_tag="VW049_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432804.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIVQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            5592..6518
                     /locus_tag="VW049_001726"
     CDS             5592..6518
                     /locus_tag="VW049_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432805.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         1963 a         1244 c         1380 g         2034 t
ORIGIN      
        1 gtcaatctat aattcacata aacagaggag tagaaaatga aaaaaaatcg tgtatttgct
       61 acagcaggtc ttgttttatt agcagcaggt gtacttgcag catgcagttc ttcaaaatca
      121 tctgattcat cagcccctaa agcttatggc tatgtttata cagcagaccc agaaaccttg
      181 gactacctga tttcaagtaa aaatagtaca acagtagtga cttcaaatgg gattgatggt
      241 ttattcacta acgataatta cggtaatctt gctcctgcag ttgcagagga ttgggaagtc
      301 tctaaggatg gtttgaccta cacttataag attcgtaaag gggttaaatg gtttacctct
      361 gatggagaag aatatgcaga ggtgacggct aaagatttcg tgaacggttt aaaacacgca
      421 gcagataaaa aatcagaagc tatgtattta gctgaaaatt cggttaaagg cttggcagat
      481 tatctatcag gaacttcaac agatttttca acagttggtg tcaaggcggt tgatgattat
      541 acgttacaat acactttgaa ccagcctgaa ccgttctgga actctaagtt gacctattct
      601 attttctggc ctctgaatga agaattcgaa acatcaaaag gaagcgattt tgctaaacca
      661 acagatccga catccttgct ttataatggt ccattcttgt tgaaagggtt gactgcaaaa
      721 tcttctgtag agtttgtaaa aaatgagcaa tattgggata aagaaaatgt ccacctagat
      781 actatcaatc tagcttacta tgatggatca gatcaggagt cgctagagcg taacttcact
      841 agtggagctt atagttatgc ccgtctttac cctaccagct ccaactattc taaggttgca
      901 gaagaataca aggacaatat ctattacaca caatcaggct ctgggattgc tggtctgggt
      961 gtgaatattg atcgccaaag ttacaactat acttctaaaa ctacagattc agagaaagta
     1021 gctactaaga aggcattgct taacaaagat ttccgtcaag ccttgaattt tgctcttgat
     1081 cgctcagctt actcagctca aatcaatggt aaagatggag cagctttagc agttcgtaat
     1141 ttatttgtaa aaccagactt tgtttcagct ggtgagaaga cctttggtga tttagtcgct
     1201 gctcaacttc ctgcttatgg tgatgagtgg aaaggtgtga atttagctga tgggcaggat
     1261 ggtttattca atgctgacaa ggccaaggca gagtttgcga aagctaagaa agctttagaa
     1321 gcagacggcg ttcagtttcc tattcatctg gacgttccag tagaccaagc atcaaaaaac
     1381 tatatatctc gtattcagtc ctttaaacaa tctgtagaaa cagttcttgg tgttgaaaat
     1441 gtcgttgttg atattcaaca aatgacaagt gatgaattcc ttaatattac ttactatgct
     1501 gccaatgctt catctgagga ttgggatgta tcgggaggag tttcatgggg gccagactat
     1561 caagacccat ctacttacct ggatatttta aaaacaacta gcagtgaaac tacaaaaaca
     1621 tatttaggat ttgataatcc aaatagccct tcagtagttc aagttggttt gaaagaatac
     1681 gataaattag ttgatgaagc tgccaaagag acaagcgact tgaatgtccg ttatgaaaaa
     1741 tatgcagcgg ctcaagcatg gttgacagat agttcactct ttattcctgc tatggcttct
     1801 tctggtgcag caccagtgct ttcacgaatt gttccattta ctggagcttc tgcgcaaaca
     1861 ggctctaagg ggtcagatgt ttacttcaaa tatttgaaat tacaagataa agcggtgact
     1921 aaggaagagt atgaaaaagc tcgtgaaaaa tggttgaaag aaaaagctga atcaaatgag
     1981 aaagctcaaa aagaattggc aagtcatgtg aagtaaataa tttctaatag aactttctct
     2041 ccatgaggag aaggttcttt tgggattttt aaaggaaata atatgaaaaa atatattttt
     2101 atgcgtgttt tgcggtcatt ggtttcgatt ttcttagtaa cgactttgac ctacacgatt
     2161 atctatacct tggttcctcg aaaattgatt ttcaagcagg atcctaacta taataaaatt
     2221 gcgacaacgg ctgataaacg tgataactat gaaaatactg tgtttgagcg tatgggctac
     2281 attgagtatt acgatactaa agagttgcaa gaaaaggcaa gtagcatgga ttcttctgta
     2341 acagtagaag caaatgcgac caataaagct atttatgaaa agtacatcaa tcaattaggt
     2401 catggttgga ctttgggaga atttactgaa agtggtcaat tctatgctac tcgtgaaatt
     2461 ccaatttttg aacgtgtttt tcacttctat gctaacttga ttgacattga ccatacaaat
     2521 aaaatccaag accctgaaaa tccagacttg aaacgctacc ttcgttttga aaatgatcca
     2581 gctatcggat ggtcattggt cggttcagga actaaacata aatatctctt gtactttaac
     2641 agtcagttcc catttgttca tcaaaacttt gtgaacttga atttaggtga ctcttaccca
     2701 acctatgcta atacaccagt tcttcaggtt attactcaag gtcaaggaca aaccaaaact
     2761 gcccaagttc agttcccaac aggtaagaaa acgtcttctg taaatattta ctcaagaacc
     2821 tacaagtcac ctagtcaggc tgactctcgt gaagtagcta gctatgggaa agatgatcct
     2881 tatacagcga ctgaaagtaa ttaccaatat ccatctatga ttgtcagctc tgctattact
     2941 ggtttgattg gtttggttct tgcctatgct cttgccgtgc cacttggttc agccatggct
     3001 cgtttcaaga acacttggat tgatagcctc tcaacagggg ctttgacctt cttgcttgct
     3061 cttccaacga ttgccttggt ttacatcgtt cgattgattg gatcatctat tgcccttcca
     3121 gattcattcc ctatcttggg agctggagat tggcgttctt acgttttacc agcagtcatc
     3181 cttggtttgt tgggtgctcc tggtacagcc atttggattc gtcgttacat gattgacttg
     3241 caatctcaag actttgttcg tttcgctcgt gcaaaaggtt tgtctgaaaa agaaatttca
     3301 aacaaacaca tctttaaaaa tgccatggtt ccgctggttt caggaattcc tggtgccatc
     3361 attggggtta tcggtggtgc aacccttact gaaacagtct tcgccttccc aggtatgggt
     3421 aaaatgttga ttgactctgt aaaagcatct aataactcta tggtcgttgg tcttgtcttc
     3481 atctttacat gtatttctat cttctcacgt cttttgggag atatttggat gactattatt
     3541 gacccacgta ttaaattgac tgagaaagga ggcaaataat gtctacaatc gataaagaaa
     3601 aatttcagtt tgtaaaacgt gacgattttg cctctgaaac tattgatgcg ccagcatatt
     3661 cttactggaa atcagtgttt aaacaattta tgaagaaaaa atcaactgta gtcatgttgg
     3721 gaatcttggt agccatcatt ttgataagtt tcatctaccc aatgttttct aagtttgatt
     3781 tcaatgatgt cagcaaggta aacgacttta gtattcgtta tatcaagcca aatgcggagc
     3841 attggttcgg tactgacagt aacggtaaat cgctctttga cggtgtctgg ttcggagctc
     3901 gtaactccat cctcatttct gtgattgcga cagtgattaa cttggttatc ggtgtttttg
     3961 tcggtggtat ttggggtatt tcaaaatcag ttgaccgtgt catgatggaa gtttacaacg
     4021 tcatctcaaa catcccacct cttttgattg ttattgtctt gacttactca atcggagctg
     4081 gattctggaa tctgattttt gccatgagcg taacaacatg gattggtatc gccttcatga
     4141 tccgtgtgca aatcttgcgc tatcgtgact tggaatacaa cttggcgtca cgtactttgg
     4201 gaacaccaac cttgaagatt gttgccaaaa atatcatgcc tcaattggta tctgttattg
     4261 tgacaaccat gactcaaatg cttccaagct ttatctcata cgaagccttc ttgtctttct
     4321 tcggtcttgg attaccgatt acagtgccaa gtttgggtcg tttgatttcg gattattcac
     4381 aaaacgtaac aaccaatgct tacttgttct ggattccatt gacaaccctt gtcttggtat
     4441 ccttgtccct tttcgtagtt ggtcaaaact tagcggatgc tagtgatcca cgtacacata
     4501 gataggagta gaaatgacaa aagaaaaaaa tgtaattttg actgctcgcg atattgtcgt
     4561 ggaatttgac gttcgtgaca aagtattgac agccattcgc ggcgtttccc ttgaactagt
     4621 tgaaggagaa gtattagcct tggtaggtga gtcaggatca ggtaaatctg ttttgacaaa
     4681 gaccttcaca ggtatgctcg aagaaaatgg tcgcattgtc caaggtagta ttgactaccg
     4741 tggtcaggat ttgacagctt tatcttctca caaggattgg gaacaaattc gtggtgctaa
     4801 gattgcgact atcttccagg acccaatgac tagtttggac cccattaaaa caattggtag
     4861 tcagattaca gaagttattg taaaacacca aggaaaaaca gctaaagaag cgaaagaatt
     4921 ggccattgac tacatgaata aggttggcat tccagacgca gatagacgtt ttaatgaata
     4981 cccattccaa tattctggag gaatgcgtca acgtatcgtt attgctattg cccttgcctg
     5041 ccgacctgat gtcttgatct gtgatgagcc aacaactgcc ttggatgtaa ctattcaagc
     5101 tcagattatt gatttgctaa aatctttaca aaacgagtat catttcacaa caatctttat
     5161 tacccacgac cttggtgtgg tggcaagtat tgcggataag gtagcggtta tgtatgcagg
     5221 agaaatcgtt gagtatggaa cggttgagga agtcttctat gaccctcgcc atccatatac
     5281 atggagtctc ttgtctagct tgcctcagct tgctgatgat aaaggggatc tttactcaat
     5341 cccaggaaca cctccgtcac tttatactga cctgaaaggg gatgcttttg ccttgcgttc
     5401 tgactacgca atgcagattg acttcgaaca aaaagctcct caattctcag tatcagagac
     5461 acattgggct aaaacttggc ttcttcatga ggatgctcca aaagtagaaa aaccagctgt
     5521 gattgcaaat ctccatgata agatccgtga aaaaatggga tttgcccatc tggctgacta
     5581 ggaggaagga aatgtctgaa aaattagtag aaatcaaaga tttagaaatt tccttcggtg
     5641 aaggaagtaa gaagtttgtc gcggttaaaa atgctaactt ctttatcaac aagggagaaa
     5701 ctttctcgct tgtaggtgag tccggtagtg ggaaaacaac tattggtcgt gctatcatcg
     5761 gtctaaatga tacaagtaat ggagatatca tttttgatgg tcaaaagatt aatggtaaga
     5821 aatcgcgtga acaagctgcg gaattgattc gtcgaatcca gatgattttc caagaccctg
     5881 ccgcaagttt gaatgaacgt gcgactgttg attatattat ttctgaaggt ctttacaatc
     5941 accgtttatt taaggatgaa gaagaacgta aagagaaagt tcaaaatatt atccgtgaag
     6001 taggtcttct tgctgagcac ttgactcgtt accctcatga attctcaggc ggtcaacgtc
     6061 aacgtatcgg tattgcccgt gccttggtca tgcaaccaga ctttgttatt gcagatgagc
     6121 caatttcagc cttggacgtt tctgtacgtg cccaagtctt gaacttgctc aaaaaattcc
     6181 aaaaagagct cggcctgacc tatctcttca tcgcccatga cttgtcggtt gttcgcttta
     6241 tttcagatcg tatcgcagtt atttacaagg gtgttattgt agaggttgca gaaacagaag
     6301 aattgtttaa caatccaatt cacccatata ctcaagcctt gctttcagcg gtaccaatcc
     6361 cagatccaat cttggaacgt aagaaggtct tgaaggttta cgatccaagt caacacgact
     6421 atgagactga taagccatct atggtagaaa tccgtccagg tcactatgtt tgggcgaacc
     6481 aagccgaatt ggcacgttat caaaaaggac taaactagta atggttttat aatttccatg
     6541 tcaattttta tggaaattat aaaacttttt ttgttggact gatttgaaaa actttgaaag
     6601 cttcctgaaa gaatttttga a
//
LOCUS       DAVKGC010000103         6502 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKGC010000103 DAVKGC010000000
VERSION     DAVKGC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.103"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..372
                     /locus_tag="VW049_001727"
     CDS             <1..372
                     /locus_tag="VW049_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9432806.1"
                     /translation="LDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHA
                     TGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGF
                     LGLALAGLLGGLGLKNKKEEN"
     gene            539..1237
                     /locus_tag="VW049_001728"
     CDS             539..1237
                     /locus_tag="VW049_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9432807.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELCKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            1234..2286
                     /locus_tag="VW049_001729"
     CDS             1234..2286
                     /locus_tag="VW049_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9432808.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            2481..3092
                     /gene="rpsD"
                     /locus_tag="VW049_001730"
     CDS             2481..3092
                     /gene="rpsD"
                     /locus_tag="VW049_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9432809.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(3186..4352)
                     /locus_tag="VW049_001731"
     CDS             complement(3186..4352)
                     /locus_tag="VW049_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9432810.1"
                     /translation="MQIKQITKKDGSIVYRANVYLGVDKVTGKDVKTSITGRTKKEVK
                     QKTKEAEIAFLQNGSTRFQASNITTYKELASLWWESYKHTVKPNTQLNVRRLLDNHIL
                     PLFGSYKLDKLTTPLIQNIVNKLADKTNKGEEGAFLYYDSLHALNKRILQYGVVMQAI
                     PFNSAREVILPRNTQKAKREKVKHFDNDELKSFLKYLDTLDLSRYRYYYEITLYKFLL
                     ATGCRINEALALSWSDIDLDNAIVHITKTLNYNQELNSPKSKSSYRDIDIDQATVSML
                     KQYKHRQVQEAWQLGRSETVVFSDFINEYPNNRTLQTRLRTHFKRADVPNIDFHDFRH
                     THASLLLNSGIPYKELQHRLGHSQLSMTMDIYSHLSKENAKKAVSFYETALKSL"
     gene            4621..4803
                     /locus_tag="VW049_001732"
     CDS             4621..4803
                     /locus_tag="VW049_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432811.1"
                     /translation="MDPDKRQKIFSQCKKEGDSLEQRKQEILEKYANKQDKPKSRKND
                     SKGSESHKRKTKSKEF"
     gene            4853..5713
                     /locus_tag="VW049_001733"
     CDS             4853..5713
                     /locus_tag="VW049_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEU9432812.1"
                     /translation="MTTNLVKQKENLEAYIRSTGYNTRGMNVENNHVLIEKPILDSYE
                     DEHQRKELVDLVNVIETRTRGGKYEVTDFESDSLQEISENFVERTESDKKKTISVDYL
                     VKLFSGKLDFSQEQLDDGQYNLTDFLGKKIIKLKRRTRNREIGKILQTAKVQTATSLD
                     DLKSIVSLINPERNVSIVISQSLFSVLEKMKDTSGNYLLKVDKETGTSETFFVDNFLI
                     VDDTTLGNEGDKKGFIGDLENFVTLFDRKKDTLSWVNANDYFRKRLILHTRFDVKKVE
                     EDCGYFIQWN"
     gene            5723..6271
                     /locus_tag="VW049_001734"
     CDS             5723..6271
                     /locus_tag="VW049_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432813.1"
                     /translation="MDINQVFDTLDDLDNKKSKIYSAREQLSEKRKSLLGIQTVSFEN
                     INSFLSNNLESLEKLEKMEKAINSLQEKYNSDFSEAKAVIFEYIFKETKQRMETKKIY
                     KQYRKKLRRILDAYDEIQELKKDVEEIHTGVVREISQKHSLSLYRTEVSPLTVLPFLN
                     PDISGWMDFSKEYRDIKEYLEK"
BASE COUNT         2150 a         1084 c         1353 g         1915 t
ORIGIN      
        1 ttggacaaca tcgtagcaac taaaaaagtc actattaaga taaatgttaa agaaacatca
       61 gacacagcaa atggttcatt atcaccttct aactctggtt ctggcgtgac tccgatgaat
      121 cacaatcatg ctacaggtac tacagatagc atgcctgctg acaccatgac aagttctacc
      181 aacacgatgg caggtgaaaa catggctgct tctgctaaca agatgtctga tacgatgatg
      241 tcagaggata aagctatgct accaaatact ggtgagactc aaacatcaat ggcaagtatt
      301 ggtttccttg ggcttgcgct tgcaggttta ctcggtggtc taggtttgaa aaacaaaaaa
      361 gaagaaaact aatcagctaa ggaaataaat gatggatagt gggctgacta agattagttt
      421 aacaactcaa tcagcaatca ggactttctt tcaatagcag attaaaatca tcgtaaaaca
      481 ataaaaatag tgttatactt aaagcagtat agcactgttt ttatcaaagg agagacagat
      541 gggaaagaca attttactcg ttgacgacga ggtagaaatc acagatattc atcagagata
      601 cttaattcag gcaggttatc aggtcttggt agcccatgat ggactggaag cgctagagct
      661 gtgcaagaaa aaaccgattg atttgattat cacagatgtc atgatgcctc ggatggatgg
      721 ttatgattta atcagtgagg ttcaatactt atcaccagag cagcctttcc tatttattac
      781 tgctaagacc agtgaacagg acaagattta cggcctgagc ttgggagcag atgattttat
      841 tgctaagcct tttagcccac gtgagctggt tttgcgtgtc cacaatattt tgcgccgcct
      901 tcatcgtggg ggcgaaacag agctgatttc ccttggcaat ctaaaaatga atcatagtag
      961 tcatgaagtt caaataggag aagaaatgct ggatttaact gttaaatcat ttgaattgct
     1021 gtggatttta gctagcaatc cagagcgagt tttctccaag acagacctct atgaaaagat
     1081 ctggaaagaa gactacgtgg atgacaccaa taccttgaat gtgcatatcc atgctcttcg
     1141 acaggagctg gcaaaatata gtagtgacca aactcccact attaagacag tttgggggtt
     1201 gggatataag atagagaaac cgagaggaca aacatgaaac taaaaagtta tattttggtt
     1261 ggatatatta tttcaaccct cttaaccatt ttggttgttt tttgggctgt tcaaaaaatg
     1321 ctgattgcga aaggcgagat ttactttttg cttgggatga ccatcgttgc cagccttgtc
     1381 ggtgctggga ttagtctctt tctcctattg ccagtcttta cgtcgttggg caaactcaag
     1441 gagcatgcca agcgggtagc ggccaaggat tttccttcaa atttggaggt tcaaggtcct
     1501 gtagaatttc agcaattagg gcaaactttt aatgagatgt cccatgattt gcaggtaagc
     1561 tttgattcct tggaagaaag cgaacgagaa aagggcttga tgattgccca gttgtcgcat
     1621 gatattaaga ctcctatcac ttcgatccaa gcgacggtag aagggatttt ggatgggatt
     1681 atcaaggagt cggagcaagc tcattatcta gcaaccattg gacgccagac ggagaggctc
     1741 aataaactgg ttgaggagtt gaattttttg accctaaaca cagctagaaa tcaggtggaa
     1801 actaccagta aagacagtat ttttctggac aagctcttaa ttgagtgcat gagtgaattt
     1861 cagtttttga ttgagcagga gagaagagat gtccacttgc aggtaatccc agagtctgcc
     1921 cggattgagg gagattatgc taagctttct cgtatcttgg tgaatctggt cgataacgct
     1981 tttaaatatt ctgctccagg aaccaagctg gaagtggtgg ctaagctgga gaaggaccag
     2041 ctttcaatca gtgtgaccga tgaagggcag ggtattgccc cagaggattt ggaaaatatt
     2101 ttcaaacgcc tttatcgtgt cgaaacttcg cgtaacatga agacaggtgg tcatggatta
     2161 ggacttgcga ttgcgcgtga attggcccat caattgggtg gggaaatcac agtcagcagc
     2221 cagtacggtc taggaagtac ctttaccctc gttctcaacc tctctggtag tgaaaataaa
     2281 gcctaaaacc cctttacaaa tccagctatt catggtagaa tagattttgt gtgaaatatc
     2341 agcaggaaag catgaagctc gtcaacaggt gtcttatgac aagtaacctt ggctgtttag
     2401 gcgaagggca tctgcacgaa tcagggcttt ctaagtgact atttccaccg aaatattatt
     2461 tatatcagga ggacattcat atgtcacgtt atacaggacc atcttggaaa caagctcgtc
     2521 gccttggcct ttcacttaca ggtacaggta aagaattggc acgtcgtaac tacgtaccag
     2581 gacaacacgg accaaacaac cgttctaaat tgtcagaata cggtttgcaa ttggctgaaa
     2641 aacaaaaact tcgtttcact tacggtgtag gtgaaaaaca attccgtaac ttgttcgtac
     2701 aagctacaaa aatcaaaggc ggaatcctag gtttcaactt tatgcttctt ttggaacgtc
     2761 gtttggataa cgttgtttac cgtcttggtc tcgcgactac tcgtcgtcaa gctcgtcaat
     2821 tcgtaaacca cggtcacatc cttgttgacg ggaaacgcgt tgatatccca tcataccgcg
     2881 taactccagg tcaagtgatc tcagttcgtg aaaaatcatt gaaagttcca gcaatccttg
     2941 aagcagtaga agctactctt ggacgtccag cattcgtatc attcgacgct gaaaaattgg
     3001 aaggttcatt gactcgcttg ccagaacgcg acgaaatcaa cccagaaatc aacgaagcac
     3061 ttgtcgttga attctacaac aaaatgcttt aattttaaga aatatcttac agaaagccta
     3121 caacagtggg ctttttgctt tgtcttaaaa tgttgatttc tgggtttgtt cagttatttg
     3181 ttcagttata aactttttag tgcagtttca tagaacgaaa ctgctttttt tgcattctct
     3241 tttgagaggt gactgtatat atccatggtc atagataatt gggaatgtcc tagacggtgc
     3301 tgaagttcct tgtaaggtat tccagagtta agcaagagac tagcgtgagt atggcgaaaa
     3361 tcatgaaaat caatgttagg tacgtcagca cgtttaaagt gagttcttaa cctggtttgt
     3421 agagttcgat tattggggta ctcatttatg aagtcagaaa agactacagt ttcagagcgc
     3481 ccaagttgcc aagcttcttg tacttgtcta tgtttgtact gcttcagcat actaacagtc
     3541 gcttgatcta tatcgatatc acgatagcta gattttgatt taggactatt gagttcttga
     3601 ttatagttta gtgtttttgt gatatgcaca atagcattat ctaagtcaat atcagaccat
     3661 gataaggcta aagcttcatt gatacgacaa ccagtagcaa gtagaaactt ataaagtgta
     3721 atctcataat aatagcgata cctacttaga tccagagtat ctaagtattt aagaaagctt
     3781 tttaattcgt cattatcaaa gtgctttact ttttctcgct ttgctttttg tgtgttgcga
     3841 ggtaatatta cctcacgagc agaattaaac ggtatagctt gcataactac accatactgt
     3901 aaaatacgct tatttaaagc gtgtaagcta tcgtagtata gaaaagcgcc ttcttcccct
     3961 ttattggttt tgtcagcaag tttattgact atattttgaa taagtggagt agttagttta
     4021 tccaacttat aagagccaaa taaaggtaag atatggttat ctagcagtct tctaacgttt
     4081 aattgtgtgt taggttttac tgtatgcttg taactttccc accataagga agctaattcc
     4141 ttgtaagtag taatatttga agcctggaat ctagtagatc cattttgtaa aaaagcaatt
     4201 tcagcttctt ttgtcttttg cttaacttct ttttttgtcc ttcctgttat gcttgtcttt
     4261 acatcttttc cagtgacttt atcaacacca agatagacat tagcacgata tactattgaa
     4321 ccgtcttttt ttgtgatttg ttttatttgc atagtattct tttccatcag caggcaagca
     4381 attagaaaag gttttgagtt tataccatgc taggagctac gagaacccct ctattttcga
     4441 ttttaagaga acgaacggta aaatgtacca ggtaagaaaa tgaagcgatt atggaactta
     4501 taaagggtat taattagata atcttctcaa ataccattgt ggcttgaatg cggtcaccac
     4561 cgcctaaacc tttgcttcca ccattggcgg ttgtgatggt atgcaggtga taagttttct
     4621 atggatccag ataaaagaca gaaaatcttt tctcagtgta aaaaagaagg tgatagctta
     4681 gaacaacgga aacaagaaat actagagaaa tatgctaaca aacaagacaa accaaaatct
     4741 agaaaaaatg attctaaagg ctcggagagt cataaaagaa aaactaagag caaagaattt
     4801 tagaaaaaat tataaacaaa aatcagatat taaaagatga aggagcaaaa aaatgacaac
     4861 taacttagtt aaacaaaaag aaaatttaga agcttatatc cgaagtacag gttataacac
     4921 tagagggatg aatgtagaaa ataatcatgt actcattgaa aaaccaatcc ttgatagtta
     4981 cgaagatgaa catcaacgta aagaactggt tgatctagta aatgttattg agactcgtac
     5041 ccgtggcggg aagtatgagg taactgactt tgaatctgat tcattacaag aaattagtga
     5101 aaattttgtt gagagaacag aatcagataa aaagaaaact atcagcgttg attacttagt
     5161 taaattattc agtggaaaac ttgatttttc acaggaacaa ttagatgatg gccaatataa
     5221 tttaacggat tttcttggta agaagattat taaattaaaa cgtagaacac gaaatagaga
     5281 gattgggaaa attctccaaa ctgcgaaagt acagaccgct acaagtctgg acgacttgaa
     5341 atctattgtt tctttaatca atccagagcg caatgtatct atagttatta gtcaatcact
     5401 atttagtgtc ttagaaaaaa tgaaagacac ttcaggaaat tatcttctta aagttgataa
     5461 agagacagga acgagtgaaa cattctttgt agataacttt ttaattgtag atgatacgac
     5521 attagggaac gaaggcgaca aaaaaggatt tatcggagat ctagaaaact ttgttacttt
     5581 gtttgatcga aagaaagata cacttagttg ggtgaatgcg aatgactatt ttagaaaacg
     5641 gttgatttta catacccgat ttgatgtaaa aaaagttgaa gaagattgtg gttactttat
     5701 tcaatggaac taggaggaag aaatggatat taatcaagta tttgatacac tggatgattt
     5761 agataataaa aaaagtaaga tttattcagc acgagaacag ttaagcgaaa aaaggaagag
     5821 tcttttaggc attcaaacag tttcatttga gaatataaat tcttttttgt caaataactt
     5881 agaatcttta gaaaagctgg aaaagatgga aaaagctatt aattctcttc aggaaaaata
     5941 taatagtgat ttttcagaag ctaaagcagt catctttgaa tacattttta aagaaactaa
     6001 gcaacggatg gaaactaaga agatctataa acaataccga aagaaactta gacgaattct
     6061 ggacgcatat gatgaaattc aagaactaaa gaaggatgta gaagaaattc atacaggtgt
     6121 agtcagggaa ataagtcaga aacattctct atcgctatat cgaacggaag taagtccgct
     6181 tactgtccta ccattcttaa accctgatat tagcgggtgg atggattttt ctaaggaata
     6241 tcgggacatt aaagagtatt tagaaaaata gggaacaaat taagtaaggc tagtgatata
     6301 tggctgaaac agaagagata tcgctagtcc tacttttatg ctttactaag tttcacataa
     6361 caaagtaagc ataaactaaa atagagtata ggcttggaag tcatatatat cagtaagtta
     6421 cagaatggag tgagtttcac agaatgtaag atatgagaaa cttaggggat aaattaaaga
     6481 aatttccctt gaacttgtca ta
//
LOCUS       DAVKGC010000104         6457 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKGC010000104 DAVKGC010000000
VERSION     DAVKGC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.104"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(76..1164)
                     /locus_tag="VW049_001735"
     CDS             complement(76..1164)
                     /locus_tag="VW049_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9432814.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(1182..1850)
                     /locus_tag="VW049_001736"
     CDS             complement(1182..1850)
                     /locus_tag="VW049_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9432815.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(1869..4307)
                     /locus_tag="VW049_001737"
     CDS             complement(1869..4307)
                     /locus_tag="VW049_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9432816.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(4458..5756)
                     /locus_tag="VW049_001738"
     CDS             complement(4458..5756)
                     /locus_tag="VW049_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9432817.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILLLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            complement(5773..6081)
                     /locus_tag="VW049_001739"
     CDS             complement(5773..6081)
                     /locus_tag="VW049_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9432818.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(6127..6447)
                     /locus_tag="VW049_001740"
     CDS             complement(6127..6447)
                     /locus_tag="VW049_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9432819.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
BASE COUNT         2102 a         1353 c          951 g         2051 t
ORIGIN      
        1 tatttcaatc ttctatagaa ttcaatacag ggatcaataa aagactacca tttggaaaac
       61 agtagtcctt attgtttatt ttgaatttac ataggcatct acagcgataa tagcttgagc
      121 aacatctgaa ggcgaaatct taaacggcat ctgatgaatt gtctcaccct ccatagttgc
      181 ttgtttacca acttttatta aatcatcata tccaacttga tccaaatgca tttcttttag
      241 agttgttggc ataccaattt ttttgtaaaa ctcaatatac ttatcaagtt cttctttagg
      301 tctattttcc aataatagtt gtactaaagt tccataagct actttttcac catgtgttaa
      361 atgatgaatg tcacctgtca atgcagtaaa accattatga attgcatgcg ccgcagctaa
      421 tcctccactt tcaaaaccta gaccactcaa taaagtatta gcttcaacaa tattttctaa
      481 tgctggtgtc accactttag cttcacaagc tgccatagcc tgtaaaccat ctgcaaacag
      541 cgtttcttca catttcttcg caattgcaac tccagccaat gtttgctgtt gtcccaacat
      601 agtttttcca tttgcctgca taaccgcacg cgcctcaacc caagttgcta aaccatctgc
      661 aataccagac gctaataaac gcttaggggc ttgtgaaata acttttgtat caaccaaaac
      721 taaatctgga tttttagaat aaaatagata atgatcaaat gcaccttcat ctgtataaat
      781 aacagataaa gcagatacag gtgcgtcggt cgatgcaatt gttggagcaa taataacagg
      841 cttttcaatc aaatctgcaa tagcttttgc gctatcaatc gtctttcccc caccaagacc
      901 gataatacta tcacaatttt ctttctcagc caaggcaaca actcgattga tttcattgtc
      961 agaagcttca ccattaaata gcgccagaac aatatggaaa ccatacctat gtaggtaatc
     1021 ttcaaatcgt tttccaacaa tatcataaac caactgatcg cataatagaa tagggcaatt
     1081 tcccaaatcc aaaattgatt tggcattttc aaacaaggca ttttccccct gaatatatct
     1141 agaaggacta gcaaaaattc tcatatgtat ttcctctcta tctaaatgga acgactattt
     1201 tgaataacaa accaatcgtc agaaaaatca tcaaccgcct tttggataga tggcatggcg
     1261 aaagctgatt caaaaacatc cgctcctgct gtaacagcat gcgcacctgc agctaaagca
     1321 ttatttactt gtgctacatt tttaaaggat gcagctaaaa tcttactagg agagttctgt
     1381 ctatcaatag caagagctaa ttgacgaatg acagaatttg aatcaatgtt cagattttcc
     1441 attctattat aatatggagc taggtaatcc gctcctactt cgatagctaa taatccctga
     1501 ataactgtat aaatagctgt tgcagtgata tggtagccct ctttttttag cacctttatt
     1561 gcacgtaatc cagctggagt aacaggtact ttgataaata tatcatctcc tgcttgtctt
     1621 cgaatttcat gagcatcctt taagatgcct tcaaaatctt gagaaatcac ctgaacatga
     1681 atagagggtg tagagccaat caattctctt acatctttga ttcgttcaaa aaaattaata
     1741 gaaccctctc tttttgcaat agtgggattt gaagttaccc cagctagcgg caaaatttca
     1801 gaccactttt taatctcatc taaatttaat gtgtcaagca taaattccat aaaaagaacc
     1861 tctttatttt acaaagtatg ctcagtacgt cctataatgt catcttgggt tgccttagaa
     1921 agaacattga agaatgcaga gtatcctgca acacgaacaa ttaagtctct gtgtttttca
     1981 ggatgtttct gagcgtcaat caacgtctct ctggaaacaa cattgtattg aatatggtac
     2041 ccatgtaaac gattaaagaa tgttcgtaac aaagcaatta gttttaattt atcttcttct
     2101 ttggctaacg tttgaggatt tactttctga tttaagagaa ccccacctac gatttcatct
     2161 gttggtaatt ttgaaacaga ttttaaaaca gatgtagggc cgtgttgatc catattatgt
     2221 gatggtgaac aaccctctgc taacggtgta cccgcgttgc gtccatctgg agttgctaat
     2281 gttccacgtc cctgccctac gttggctgat atagaagatg ttcctgaata acgaattcct
     2341 ccaataggcc ctcttccata acgtgtatta ggatatttag caatttcatc aacataaatg
     2401 tcataagcag cagtaaccaa tttgtcagca taatcatcat cattaccata cttaggtgca
     2461 tcatgaatca acatttcttg aatgacctta ccttcttctc cggcataatc tgtttccagt
     2521 gcatgccaaa gttgacttgg gcttatacgt tcttcctcaa acaccaattt tttaattgca
     2581 gctaacgaat ccgacaaatt tgcaattcca acttgcaatc ctgatatata atcatatact
     2641 gctccacctt ctttaaggtg ttttccacga ccaatacaat catcagtcaa tgctgaacat
     2701 agaatatcag gaacttctcg ttccaatgat aaatcaatag aattttcaac aataacactc
     2761 attcgtgtca aatatcttag tgttttatcc caagcatttt ctaattcaga aaagttcttc
     2821 atatccttaa aatgaccaaa gcttggtgca aaccgtttac ccgaagccgg atcaattcca
     2881 tcattcatcg tgataagtag aaccttaggg aagttcatat aactcatacc tgtgcaacga
     2941 tagccccatt tccctggaac tgccgtttca acacatccaa tggcactgta atcataagca
     3001 tcatcttcca atactccttt tgcaataaaa gaaggaataa taatctcatc attattaaat
     3061 gcaggcatac caaaaccaag tttcatcact tcaatacact cattcatgaa acgagcatct
     3121 aaacctgcat ggtaacgtac agttagatta ggttgcggta gatgggtttg tgcaactgat
     3181 tttaatacca aataagataa tgggttaaca gcatccttct tatctcgagt ctgtccacca
     3241 attgtaacat tttgatataa aggacttcct gctgaagaaa atgtatgtga ttgactgcga
     3301 accttattaa ttgtaattgt cttaatccaa agatttgtca gacgttcaac aatgctatct
     3361 tctgtttctt taccactttc taaatcagcc ttcatatatg gatacatata ttgatcaaaa
     3421 cggccatatg aaagagagtg gccattagat tcaatttgta aaatacattg aataaaccaa
     3481 actgattgaa tagcttctgc aaaagtagtt gccggttcat atgggactct agagcaaata
     3541 tctgcaatct caagtaattc tttcttacgt ttaggatttg cattttcggc taaactttta
     3601 gcaagagcaa caaagcgctt tgcatatact ttaatagcat cgattacgat aaatatagag
     3661 tcgtaaaaat gatatttatc aatacttgct ggatctgtta aatctagagc tacttttgct
     3721 ttacgagccc gctcttcaaa acctcttaaa ccaaattgca aaagtttctg atagttaact
     3781 gctaagtgag catctccaga attcatctta ccttccatac cgaagaatcc tgtttccata
     3841 taaacagaca cttcttcagg taataaggca ccagctctag cacgtaaatt attattttcc
     3901 caaaacggag caatacttct aagttgttct tttgtttctt ctgtaatata gaaaacatct
     3961 ccatcacgct tttcaaaaag atccaactca ttgagaacaa attctagcgt atattccgga
     4021 aaaataggag catctttatt ggaagaagct tgatttcccg caatcataga ttcttcttca
     4081 atatagatag acatattttc caaaatttct ttcagcatat atgcacgttt taggacatta
     4141 ggttgttcct tgtatcgttc ataggcgcgt gttgctagaa ctgctctctc agcatcaata
     4201 taaggttttt tatttaaaac atcttctcga tatttgttca tcctttcagt aaggctgcca
     4261 aaatattctg tcttgattgt tgttcttgct acttctgtat ttaccatcgt aagcaccttt
     4321 ctttcgaaaa tatttttatt tctttcgaaa atataatacc gatttttata gaaattttca
     4381 agcttttaac tagaaaaatt ttaactctct aatttttagc acaaatgtgt gagtttaaca
     4441 gtaacacaca aatacctcta agattgtttg atttcatttt ggtaagctaa acgatcttgt
     4501 atcttaaaga atggaaagta aaccactgta gatatcacca atactaataa ctgagtcaca
     4561 acaccttgcc atccacccac caaaaatcct gatagaatag ctggtgtact ccaaggcaat
     4621 gttacccctg agaatggctg catgaaacct gttgcaatag ctccatatac tatcacagct
     4681 gcaagtacag gaacaagaat gaaaggtaca aacataactg gattcatgac aatcggaaat
     4741 ccaaatacaa ctggctcatt tacgttaaat attgctggaa aagctgcaac ttttcctaag
     4801 gcttggtatt gttttgattt tgctgcaaaa agcatggcaa ctacaagacc aaacgtaatc
     4861 cctgaacctg atagaattaa aaatgaatct aaaaattgtt gagtaacaat atgtgcacca
     4921 ttttctaatg atagattacc agaggctaac atagctttat tagcatcaag attagataaa
     4981 agcagagctg tcactactcc atttactacc gattgcccat gaacaccaaa ccaccacaaa
     5041 aatgatataa agaatgcaat tccaatagca ccatacaaag atccagttaa accttgcaac
     5101 ggaacttgaa tagcagaata aatcatttct atgaatgttc cgccattagt caatgacttc
     5161 gctaaaatat atacaatcat agaagataag aaaattacaa atgctggaat cattgcttca
     5221 aactgtttcg caatagcttg tggaacttgt tctggcatct taataacaat ttttctcttt
     5281 ataaagaagg tataaatact tcctactacc aaacctataa tgatagcacc gataattcct
     5341 tggcctccaa accaaacttt actaatagcg tccccaatcg cctcaccttg tttagggata
     5401 taagatgatc ttagcaaaat aaagaatgca gatacagata gaactccagc tggtaaagcc
     5461 tctactccgc tattcttagc ataagaatag gcaattgaaa aacaagaaat tagacccata
     5521 atagcaaaag ttcctgaata tacttgcata aacggctctg tccaattagc tccaaaaaca
     5581 ctagcaatgc tcttatttaa tccttcgaac ggcaattgtc ccataatcaa gaacaaacta
     5641 ccaactactg tcaatagcaa aattgctaac atcccatctt ttagagctat aatgccacgc
     5701 atattcacaa acttcatcat cggtgcaatg attttctgaa catccatctt tgacataata
     5761 aatctccttt tcttacccac taatcaaaga tagggccaaa tctaatactt ttttcccatc
     5821 taacatacca tagtccatca tcggaataac agctatcgga acatcacact tatcacaaat
     5881 ttcttttgat ttatctaatg tataagcaac ttgtggaccc aatagtgcaa catctatatt
     5941 tggcgcataa tccgctaatt tagactgaga aaacgcctct atttctgcct caactccact
     6001 agattgcgct gcaattttca tattatttac aagcatacca gtagaaaaac ctgctgcaca
     6061 aaacaaacca atcttcacca ttatgttttc ctcctctatg ttaataacaa tgataatact
     6121 ctagtattat tttttatgaa gttcttttct caaactaata atttctttga ttaaattaat
     6181 ctccgtcata ctagtcatga gatgatcttg tgaatgaaca aagagagctg taatttctgt
     6241 ctttgtacca gacgcttcct gtgccaagaa ttttgtttgt agattatgag cttctaataa
     6301 ggctgcatca gctaactgga tttcttcttc acatcgttca catgtaccgt tctttatgta
     6361 atctaatgct ttataaatat gttgtttcgc atcgccagca tataaaatta aacccatgat
     6421 aatttgatct ggaacaataa tttccataaa ttcctcc
//
LOCUS       DAVKGC010000105         6398 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKGC010000105 DAVKGC010000000
VERSION     DAVKGC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.105"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            1..100
                     /locus_tag="VW049_001741"
                     /pseudo
     CDS             1..100
                     /locus_tag="VW049_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            253..453
                     /locus_tag="VW049_001742"
     CDS             253..453
                     /locus_tag="VW049_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432820.1"
                     /translation="MVVSASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            478..895
                     /locus_tag="VW049_001743"
                     /pseudo
     CDS             478..895
                     /locus_tag="VW049_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1126..1833
                     /gene="ccdA2"
                     /locus_tag="VW049_001744"
     CDS             1126..1833
                     /gene="ccdA2"
                     /locus_tag="VW049_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9432821.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQERAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1844..2401
                     /gene="sdbB"
                     /locus_tag="VW049_001745"
     CDS             1844..2401
                     /gene="sdbB"
                     /locus_tag="VW049_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9432822.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDVPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2775..4166
                     /locus_tag="VW049_001746"
     CDS             2775..4166
                     /locus_tag="VW049_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9432823.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4309..5226
                     /gene="adcAII"
                     /locus_tag="VW049_001747"
     CDS             4309..5226
                     /gene="adcAII"
                     /locus_tag="VW049_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9432824.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5234..>6398
                     /gene="phtB"
                     /locus_tag="VW049_001748"
     CDS             5234..>6398
                     /gene="phtB"
                     /locus_tag="VW049_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEU9432825.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANTAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEEPSPQLTPEPSPSPQPAPNPQPAPSNPIDEKLVKE"
BASE COUNT         1960 a         1182 c         1338 g         1918 t
ORIGIN      
        1 tccaatgact tacgaagaga cgatgacgag cgactttttt gaagcttggt ttcagaagtt
       61 tttcttacca acattaaaca caccatcggt tattatagta aaatgaaata agaacaggac
      121 aatcaaatca atttctagca atgttttaga agtccatatg tactattcta gtttcaatat
      181 accataactg ctcctagtca acctgcaagt cctgtcccag ctccaagctc agctccgact
      241 caagatccta gagtggtagt aagcgcttcg gctagctcaa cgagcacaca agctcaagag
      301 caagttgaca agtctgaact tcgtgccttg agtcaagagt tagaccaacg cttgaaagct
      361 ttggcaacag tatctgatcc gaaaatcgat gcaaccaagg ctgtcctcct agatgctcaa
      421 aaagctccgg aagatagtgc cttgacagag taaggtctgc atactgcagt agagtctgtg
      481 aagactgcct tggactctct caaagatgtg atggcaaatg ctagcgacag caagcctaca
      541 caggacaaga aggatgcaaa acaaggaacg gaagatagta aggattcaga taagatgact
      601 gaaacaaact cagttccagc aggagtgatt gtggtcagtc tacttgccct cctaggcgtg
      661 attgccttct ggctaattcg ccgtaagaaa gagtcagaaa tccagcaatt aagcacggaa
      721 ttgaccaagg ttctaggaca gctagatgca gaaaaaagcg gataaaaaag tccttgccaa
      781 agcccaaaac cttctccaag aaacccttga tttcgtgaaa taagaaaatg gctcagcaga
      841 gacagaaact aaactagtag aggagcttaa agcaatcctt gacaaactca agtaaaacat
      901 gatttatgaa atgtcagacc taaaagacat tttcgttcac aaaagcagcg acgagtaatc
      961 cttgtcgttg ctttttggct attttggggg ataagatccg ccttgactag caaaagaaaa
     1021 gattggtcca atagtgcccg atacgatttt gcgtagcaga aagcttacaa ataaaattga
     1081 atatagtaaa ataggattag aattattaag aaagttggtt ctttattgga aacgatagta
     1141 tttttaatct ctgtttttct agcaggtgtt ttatcctttt tttctccttg tatttttcct
     1201 cttctgccag tctatgctgg gattttattg gatgatcaag aaagggcaaa aagcttttct
     1261 ttgtttggga gaaaggttct ctggtcaggc ttgattcgaa cactttgctt tatcgctggt
     1321 atctctctca ttttctttat tctaggcttt ggtgctggtt actttggtca tattctctat
     1381 gcaaattggt ttcgatatgt catgggagct attattatca ttttgggtct tcaccagatg
     1441 gaaatttttc atttgaagaa attagaagtt caaaaaagtt ttacctttaa aaaatcagat
     1501 tctaatcgtt attggtcagc ttttttactt ggtattacct ttagctttgg ttggacacct
     1561 tgtattggtc cagttttaag ttctgtttta gcacttgcgg cttctggagg caatggtgct
     1621 tggcaaggag cgatttatac tctcatttac actctgggca tggcccttcc tttcttggtc
     1681 ttggcactag cttcaggtct agtcatgcca tattttagta aaatcaagcg tcatatgatg
     1741 ctactaaaga aaattggtgg tttcctcatt gttttaatgg gaattttgtt actattagga
     1801 caagtaaatg ttctagctgg aatttttgaa taaaggagga caaatgaaaa aagtaatgtt
     1861 tgctggctta agtctcttgt cattagttgt attgatggcc tgtggtgagg aagaaactaa
     1921 aaagactcaa gcagcacaac agccaaaaca acaaacgact gtacaacaaa tttctgttgg
     1981 aaaagatgtt ccagacttta cattgcaatc catggatggc aaagaagtta agttatctga
     2041 ttttaagggt aaaaaggttt acttgaagtt ttgggcttca tggtgtggtc catgcaagaa
     2101 aagtatgcca gagttgatgg aactagcggc gaaaccagat cgtgatttcg aaattcttac
     2161 tgtcattgca ccaggaattc aaggtgaaaa aactgttgag caattcccac aatggttcca
     2221 agaacaagga tataaggata tcccagttct ttatgatacc aaagcaacca ccttccaagc
     2281 ttatcaaatt cgaagcattc ctacagaata tttaattgat agccaaggaa agattggaaa
     2341 gattcaattt ggtgctatca gtaatgcgga tgcagaagca gcatttaaag aaatgaacta
     2401 gaatcgataa aatctgatta caagatgtag tcagattttg gtaacttcta aaactaactt
     2461 ccagtttccc acaacctagc ttttagtatg agaaaaacgc gtgacatcag tggaaatccg
     2521 tcttagacta acttccactg gttttctttt tcttgatata taagggttca aaagcgaaaa
     2581 gactcagaaa aagtgcacga caaatagccc taaaacagag tcgtcttaga gtaacttcca
     2641 gttttgggag agagatggaa gttactttga gaagttacgg tagtcagatt tttttagaaa
     2701 aacattttat aaatattcac atatctccta tatttatggt aaaatagaat tatcagttta
     2761 ttttggagtc aaagatgaat atatttagaa caaagaatgt tagtttagat aaaacagaga
     2821 tgcataggca tttgaagtta tgggatttga ttttgctggg tatcggagcc atggtaggga
     2881 caggcgtctt tacaatcaca ggtactgcag ctgcaacact tgctggccca gccctagtga
     2941 tttcaatcgt tatttctgcc ttgtgtgtgg gattatcagc cctctttttt gcagaatttg
     3001 cctcgcgagt acccgctaca ggaggtgcct atagttacct ctatgctatc ttaggagaat
     3061 tccctgcctg gttggctggt tggttaacca tgatggagtt catgacagcc atatcaggcg
     3121 tagcttcggg ttgggcagct tattttaaag gcttgctctc tcaatacggg atagcctttc
     3181 ctcaggcttt aaatggtacc tttaatcctc aagcagggac atttgttgat ttattgccta
     3241 ttcttgtctt ggtcttggtg acttcgcttg ttttactaaa tgccaaggca gccttacgct
     3301 ttaattcgat tctagttatt ttgaaatttt ccgctttagc tctctttgtt ttagtaggaa
     3361 tttggaatat taagtttgat aattggagca attttgctcc ttacggtttt ggacaaatct
     3421 atggagctag tactggtatt atggctggtg catccttgat gttcttcggt tttttgggat
     3481 ttgaatccat ctctatggct gtagatgaag tcaagactcc tcaaaaaaat attcctagag
     3541 ggattgtctt atcgctttct atcgtaacca ttctctatgc cttggtgacc cttgttttga
     3601 ctggtgtggt tcactatagt catttaaatg tcgatgatgc cgttgccttt gcccttcgta
     3661 gtgttgggat tagttgggca gccaactatg tgtcattagt ggctatcttg accttgatta
     3721 cagtttgtat ttcgatgacc tatgccctat cgcgtatgat ttacagttta gcacgtgacg
     3781 gcttagtgcc tgctgccttt aaggaactga cgaagactag caagatacca aagaatgcta
     3841 ctatcttgac aggtctagct tcagcagtag cagcaggaat gttcccacta gccagtattg
     3901 cagccttttt aaatatttgt accttagcct acttgatcat gttggcttat ggtctgattc
     3961 gcttacggaa agaaaaagga atgcccaaag ctggggaatt taaaacacca ttggtacctc
     4021 tgttaccaat tttatcgatc atcatttgtt tatccttcat gttacagtat aatatgaata
     4081 cctggattgc tttcctagtt gcgttgttag taggaagtat tatttacttc acttatggct
     4141 ataagcattc taccatagaa gaataaagtg aaagtctatt gagttgttga cactcagcag
     4201 atttttatat gtgaaagaaa tttcaaattt tttctaaaaa tatcttgaca gattaaattt
     4261 tcaggagtag aatatttact agttaattaa atggttaagg agttgttcat gaagaaacaa
     4321 aatttatttt tagtcctgtt aagtgtcttt cttttgtgct tgggggcttg tggtcaaaag
     4381 gaaagtcaga caggaaaggg gatgaaaatt gtgaccagtt tttatcctat ctacgctatg
     4441 gttaaggaag tatctggtga cttgaatgat gttcggatga ttcagtcaag tagtggtatt
     4501 cactcctttg aaccttcggc aaatgatatc gcagccatct atgatgcaga tgtctttgtt
     4561 taccattctc atacactcga atcttgggca ggaagtctgg atccaaatct aaaaaaatcc
     4621 aaagtgaagg tcttagaggc ttctgaggga atgaccttgg aacgtgtccc tggactagag
     4681 gatgtggaag caggggatgg agttgatgaa aaaacgctct atgaccctca cacatggcta
     4741 gatcctgaaa aagctggaga agaagcccaa attatcgctg ataaactttc agaggtggat
     4801 agtgagcata aagagactta tcaaaaaaat gcgcaagcct ttatcaaaaa agctcaggaa
     4861 ttgactaaga aattccaacc aaaatttgaa aaagcgactc agaaaacatt tgtaacacaa
     4921 catacagcct tttcttatct agcgaagaga tttgggctta atcaacttgg tattgcaggt
     4981 atctctcctg aacaagaacc aagtccacga caactaacag aaattcagga atttgttaag
     5041 acctataagg ttaaaacgat ttttacagaa agtaacgctt cttcaaaagt agctgaaact
     5101 cttgtcaaat caacaggtgt gggtcttaaa actctgaatc ctttagagtc agacccacaa
     5161 aatgacaaga cctatttaga aaatcttgaa gaaaatatga gtattctagc agaagaatta
     5221 aagtgaggaa agaatgaaaa tcaataaaaa atatctagca ggttcagtgg cagtccttgc
     5281 cctaagtgtt tgttcctatg aacttggtcg tcaccaagct ggtcaggtta agaaagagtc
     5341 taatcgagtt tcttatatag atggtgatca ggctggtcaa aaggcagaaa atttgacacc
     5401 agatgaagtc agtaagaggg aggggatcaa cgccgaacaa atcgtcatca agattacgga
     5461 tcaaggttat gtgacctctc atggagacca ttatcattac tataatggca aggtccctta
     5521 tgatgccatc atcagtgaag agctcctcat gaaagatccg aattatcagt tgaaggattc
     5581 agacattgtc aatgaaatca agggtggtta tgtcattaag gtaaacggta aatactatgt
     5641 ttaccttaag gatgcagctc atgcggataa tattcggaca aaagaagaga ttaaacgtca
     5701 gaagcaggaa cgcagtcata atcataactc aagagcagat aatgctgttg ctgcagccag
     5761 agcccaagga cgttatacaa cggatgatgg gtatatcttc aatgcatctg atatcattga
     5821 ggacacgggt gatgcttata tcgttcctca cggcgaccat taccattaca ttcctaagaa
     5881 tgagttatca gctagcgagt tagctgctgc agaagcctat tggaatggga agcagggatc
     5941 tcgtccttct tcaagttcta gttataatgc aaatacagct caaccaagat tgtcagagaa
     6001 ccacaatctg actgtcactc caacttatca tcaaaatcaa ggggaaaaca tttcaagcct
     6061 tttacgtgaa ttgtatgcta aacccttatc agaacgccat gtggaatctg atggccttat
     6121 tttcgaccca gcgcaaatca caagtcgaac cgccagaggt gtagctgtcc ctcatggtaa
     6181 ccattaccac tttatccctt atgaacaaat gtctgaattg gaaaaacgaa ttgctcgtat
     6241 tattcccctt cgttatcgtt caaaccattg ggtaccagat tcaagaccag aagaaccaag
     6301 tccacaactg actccagaac ctagtccaag tccgcaacct gcaccaaatc ctcaaccagc
     6361 tccaagcaat ccaattgatg agaaattggt caaagaag
//
LOCUS       DAVKGC010000106         6264 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKGC010000106 DAVKGC010000000
VERSION     DAVKGC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.106"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            55..450
                     /locus_tag="VW049_001749"
     CDS             55..450
                     /locus_tag="VW049_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9432826.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            461..832
                     /locus_tag="VW049_001750"
     CDS             461..832
                     /locus_tag="VW049_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9432827.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKNPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            842..3085
                     /locus_tag="VW049_001751"
     CDS             842..3085
                     /locus_tag="VW049_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9432828.1"
                     /translation="MTEIVKASLENGIQKIRIRSEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFFCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            3292..5067
                     /gene="spxB"
                     /locus_tag="VW049_001752"
     CDS             3292..5067
                     /gene="spxB"
                     /locus_tag="VW049_001752"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9432829.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            5178..5525
                     /locus_tag="VW049_001753"
     CDS             5178..5525
                     /locus_tag="VW049_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9432830.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAALVLDFYRMK"
     gene            complement(5642..>6264)
                     /locus_tag="VW049_001754"
     CDS             complement(5642..>6264)
                     /locus_tag="VW049_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9432831.1"
                     /translation="ILRKQTLKPLSRLKIKSQFKKTRKRFKTSRPFHKVRLMYQDEAG
                     FGRISKLGSCWAPIGVGPHVHSHYIREFRYCYGAVDAYTGESFFLRAGGCNTEWMNVF
                     LEELSQAYPDDYFLLVMDNAIWHKSSILKIPTNIGFAFIPPYTPEMNPIEQVWKEIRK
                     RGFKNKAFRTSEDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLFESR"
BASE COUNT         1849 a         1191 c         1410 g         1814 t
ORIGIN      
        1 ttgggagtat actgttgagt gtaattgaca aatgtagatt ttggaggtat ggtaatgcag
       61 atttcagatg cagaatggca ggtcatgaag attatttgga tgcaggggga gcagaccagt
      121 acagatttga ttagggtttt ggcagagcgg tttgactggt ccaagtccac ggttcagaca
      181 cttttggctc gtctagttga gaaagagtgt ttgactcgga aaaaagaagg caagtccttt
      241 gtttattcag cgcttttaac tctagaccaa agtcgggatt tacttgtcca agatatcaaa
      301 gacaaggttt gttcccgtag gattaggaat ttgttggctg acttgattgt agaatgtgaa
      361 tttactcaga ctgacttgga agacttggaa gctgtgattt cagagaagaa atcaagtgct
      421 gtaacagaag taagatgtaa ttgtatgtaa aggagacgtc atgttaaata gtattgtaac
      481 cattatttgt attgccctta tcgcgtttat cttgttttgg tttttcaaaa atcctgaaaa
      541 atctggacaa aaagcccagc aaaaaaacgg ataccaagag attcgagtgg aagtcatggg
      601 aggctatact cctgagttga ttgtcctcaa gaaatcagtg ccagcccgca ttgtctttga
      661 ccgcaaggat ccttcaccat gtctggatca aattgttttt ccagattttg gtgtacatac
      721 gaacctgcca atgggggaag agtatgtagt ggaaatcacg cctgaacagg ctggagaatt
      781 tggctttgct tgtggtatga acatgatgca cggcaagatg attgtagagt aggtggagac
      841 tatgacagaa attgtgaaag caagcttaga aaatggcatt caaaaaatcc gtatccgatc
      901 tgaaaaaggc tatcatccag cccatatcca gcttcaaaag ggaattccag ctgagattac
      961 ctttcatcgt gctactcctt caaactgtta taaggaaatt ctgtttgaag aagaaggtat
     1021 cttggaacca atcggcgtag atgaggagaa agtcattcgt tttacacctc aagaattagg
     1081 gcgacatgaa tttttttgtg gcatgaagat gcaaaaggga agctatacag tcgttgagaa
     1141 gactcgaaaa tctctatctc tcctgcaacg tttttggatt actagtatct ttactgtgcc
     1201 tcttgtgatt ctcatgattg ggatgttgac aggtagcatt agtcatcaag tcatgcattg
     1261 gggaaccttt ctagcaacaa cgcctattat gttagttgca ggtaagccat atatccagag
     1321 tgcttgggcc agttttaaaa agcacaatgc caacatggat accttggttg cgctgggaac
     1381 tctagtggct tatttctata gcctagttgc tctctttgct ggtctccctg tttacttcga
     1441 aagtgctgga tttatcctct ttttcgttct tttgggagca gtttttgagg aaaaaatgag
     1501 aaaaaatacg tcccaagctg tggagaaatt actggacttg caagctaaaa ccgcagaagt
     1561 cttgagtgat gatagttatg tccaagttcc tttggaacaa gtcaaggtag gcgacctgat
     1621 tcgagtgcgt cccggtgaaa agattgctgt tgatggtgtc gtagtagaag gtgtctctag
     1681 tattgacgaa tccatggtga caggtgagag tctgcctgtg gacaagacag ttggagatac
     1741 tgtcattggc tcaaccatca atcatagtgg aacgcttgtc tttagagcag aaaaagttgg
     1801 ctcagagact gttttggctc agattgtgga ttttgtgaag aaagctcaga caagtcgtgc
     1861 gccgattcag gacttgacgg ataagatttc agggattttt gtcccagtag ttgtcatttt
     1921 aggaatcatg accttttggg tttggttcgt cttgctcagg gatagtgtgg ttgtgcttgg
     1981 agctagcttt gtgtcctctc ttctctacgg agtggcggtt ttgattatcg cctgtccttg
     2041 tgccttggga cttgcaacac cgacagccct tatggtgggg acaggacgta gtgccaagat
     2101 gggggttctc ctcaaaaatg gaactgtctt acaggaaatc cagaaagttc aaacccttgt
     2161 ctttgataag accgggactt tgacggaagg gaaacctgtg gtaacagatg tcatcggcga
     2221 cgaagtagaa gtgtttggat tggcagcctc cttggaagat gcttctcaac acccactggc
     2281 tgaggctatc gttaagcgag cgagtgaagc tggacttgag tttcaaactg ttgaaaattt
     2341 tcaggccttg cacgggaaag gtgtttcagg gcgaatcaat ggaaaacaag ttttacttgg
     2401 aaatgctaaa atgctggatg gcatggatat ttctaatact tatcaagata aactagaaga
     2461 actagaaaaa gaagctaaga cagttgtgtt cttagctgtt gacaatgaaa tcaaaggctt
     2521 gcttgctttg caagatattc ctaagaaaaa tgctaagcta gccatcagtc agctaaaaaa
     2581 acgtggtctc gggacagtca tgctgacagg agacaatgct ggtgtggcgc gtgctattgc
     2641 agatcaaatc ggaattgaag aggtcattgc aggcgtcttg ccagaagaaa aagcccatga
     2701 aatccataaa ctgcaacagt caggtaaagt agcctttgtt ggtgatggga tcaatgacgc
     2761 tcctgccctt agtgtagcag atgtggggat tgctatggga gctggaacag atatcgccat
     2821 cgagtcagca gatttggtgt tgacaaccaa taatctttta ggagtggtgc gtgccttcga
     2881 catgagtaag aaaacctttc atcgtattct gctcaatctt ttctgggctt ttatctacaa
     2941 tgttgtcgga attccgattg cagcaggagt cttttcaggt gttggactgg ctctcaaccc
     3001 agaattggca ggtctagcca tggcctttag ttctgtatct gttctgacca gttcccttct
     3061 gttaaatttt agtaaaatag actaaaagta atgactagat ttttttgtta taaaacagaa
     3121 atgacgacac tttcaaaact gtattagaat agagaataga gagttttgag cagattttta
     3181 gaaaagtcag cataaatatg atacagtgga atagtaaaaa tttggagaac gtttccaatt
     3241 ctatgtaatc gtattctcca agtctaaaaa aattgaagga gagttatcat tatgactcaa
     3301 gggaaaatta ctgcatctgc agcaatgctt aacgtattga aaacatgggg cgtagataca
     3361 atctacggta tcccatcagg aacactcagc tcattgatgg acgctttggc tgaagacaaa
     3421 gatatccgct tcttacaagt tcgccacgaa gagacaggtg ctcttgcagc ggttatgcaa
     3481 gctaaattcg gcggctcaat cggggttgca gttggttcag gtggtccagg tgcgactcac
     3541 ttgattaacg gtgtttacga tgcagctatg gataacactc cattcctagc aatccttgga
     3601 tcacgtccag ttaacgaatt gaacatggat gctttccaag agcttaacca aaacccaatg
     3661 tacaacggta tcgctgttta caacaaacgt gtagcttacg ctgagcaatt gccaaaagta
     3721 attgacgaag cctgccgtgc tgcagtttct aaaaaaggtc cagctgttgt tgaaattcca
     3781 gtaaacttcg gtttccaaga aatcgacgaa aactcatact acggttcagg ttcatacgaa
     3841 cgctcattca tcgctcctgc tttgaacgaa gttgaaatcg acaaagctgt tgaaatcttg
     3901 aacaatgctg aacgcccagt tatctatgct ggatttggtg gtgttaaagc tggtgaagtg
     3961 attactgaat tgtcacgtaa aatcaaagca ccaatcatca caactggtaa aaactttgaa
     4021 gctttcgaat ggaactatga aggtttgaca ggttctgctt accgtgttgg ttggaaacca
     4081 gccaacgaag tggtctttga agcagacaca gttcttttcc ttggttcaaa cttcccattt
     4141 gctgaagttt acgaagcatt caagaacact gaaaaattta tccaagtcga tatcgaccct
     4201 tacaaacttg gtaaacgtca tgcccttgac gcttcaatcc ttggtgatgc tggtcaagca
     4261 gctaaagcta tccttgacaa agtaaaccca gttgaatcaa ctccatggtg gcgtgcaaac
     4321 gttaagaaca accaaaactg gcgtgattac atgaacaaac tcgaaggtaa aactgagggt
     4381 gaattgcaat tgtatcaagt ttacaatgca atcaacaaac atgctgatca agacgctatc
     4441 tactcaatcg acgtaggtaa cactactcaa acatctactc gtcaccttca catgacacct
     4501 aagaacatgt ggcgtacatc tccactcttt gcgacaatgg gtattgccct tcctggtggt
     4561 atcgctgcta agaaagacaa tccagatcgc caagtatgga acatcatggg tgacggagca
     4621 ttcaacatgt gctacccaga cgttatcaca aacgttcaat acgaccttcc agttatcaac
     4681 cttgtcttct caaatgctga gtacggcttc atcaagaaca aatacgaaga tacaaacaaa
     4741 cacttgtttg gtgtagactt cacaaacgct gactacgcta aaattgcgga agctcaagga
     4801 gctgttggat tcacagttga ccgtatcgaa gacatcgatg cagttgttgc agaagctgtt
     4861 aaattgaaca aagaaggtaa aactgttgtc atcgatgctc gcatcactca acaccgtcca
     4921 cttccagtag aagtacttga attggatcca aaacttcact cagaagaagc tatcaaagcc
     4981 ttcaaggaaa aatacgaagc agaagaactc gtaccattcc gtctcttctt ggaagaagaa
     5041 ggattgcaat cacgcgcaat taaataattc ctctcgccga aaatcaaata tgaaacttgt
     5101 agactttatt ctttgatttt catagagcag aactttaaaa gatcggagca atccggtctt
     5161 tttatggagg agagtaaatg aatttaaatc aattagatat tatcgtttca aatgttcccc
     5221 aagtctgtgc tgacttggag catatcttgg ataaaaaggc agattatgct gatgatggtt
     5281 ttgctcagtt tacgattggt agtcactgcc ttatgttgtc acaaaatcat ttggttcctt
     5341 tggaaaactt tcagtcagga atcattattc atatcgaggt tgaggatgta gaccagaact
     5401 acaaacggtt gaacgagctt ggtataaagg ttttacacgg accaactgta accgattggg
     5461 gaacagagtc cttattagtt caaggacctg ctgctctagt gcttgatttt tatcgtatga
     5521 aataggtcgc gtagtcattt gacgttaacc ttagctattt ttaaaataga aattgagaat
     5581 atgaactatt gaaagatcgg agcaatccgg tctttttata taaagaaatt gcatataata
     5641 ctcatctgct ttcaaaaagc attctagtcc atctccgatt aacgatggac tttatcacct
     5701 ccttctccag tccttgtatg acatcttgaa gttgattcat gacatcttcc gaagttcgaa
     5761 aggctttatt cttaaatcca cgtttacgaa tctctttcca cacttgttca atggggttca
     5821 tctctggtgt gtatggagga ataaatgcaa agccaatatt agtcggaatc tttaagatac
     5881 ttgatttatg ccatatagca ttgtccataa cgagtaaaaa ataatcatct ggataagctt
     5941 gtgaaagctc ttctaaaaag acgttcatcc actcagtatt acatccacca gctcttaaga
     6001 aaaatgattc gcctgtatag gcatcaacag ctccataaca atagcgaaat tctcgtatat
     6061 agtgactatg gacatgtgga cctactccta ttggagccca acaagatccc agtttactga
     6121 ttctaccgaa accagcctca tcttggtaca tcaagcgaac cttatgaaaa ggtctactgg
     6181 ttttaaagcg ctttcttgtc ttcttgaatt gagattttat ttttagacgc gacaatggtt
     6241 tgagcgtctg ctttcttagg atgt
//
LOCUS       DAVKGC010000107         6221 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKGC010000107 DAVKGC010000000
VERSION     DAVKGC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.107"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            1..305
                     /locus_tag="VW049_001755"
                     /pseudo
     CDS             1..305
                     /locus_tag="VW049_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(460..783)
                     /gene="tnpB"
                     /locus_tag="VW049_001756"
     CDS             complement(460..783)
                     /gene="tnpB"
                     /locus_tag="VW049_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9432832.1"
                     /translation="MTIHLSSLGEVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLIWLSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(764..949)
                     /locus_tag="VW049_001757"
     CDS             complement(764..949)
                     /locus_tag="VW049_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432833.1"
                     /translation="MPQPIVPVEIPQSRRFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(1253..2815)
                     /gene="guaA"
                     /locus_tag="VW049_001758"
     CDS             complement(1253..2815)
                     /gene="guaA"
                     /locus_tag="VW049_001758"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9432834.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2957..3655
                     /locus_tag="VW049_001759"
     CDS             2957..3655
                     /locus_tag="VW049_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9432835.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKYGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3698..4594)
                     /locus_tag="VW049_001760"
     CDS             complement(3698..4594)
                     /locus_tag="VW049_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9432836.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(4603..5538)
                     /locus_tag="VW049_001761"
     CDS             complement(4603..5538)
                     /locus_tag="VW049_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9432837.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5535..>6221)
                     /locus_tag="VW049_001762"
     CDS             complement(5535..>6221)
                     /locus_tag="VW049_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteinase"
                     /protein_id="HEU9432838.1"
                     /translation="KANNRKLNETFYIETLGMKALLEESAFLSLGDQTGLEKLVLEEA
                     PSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQNGYAFEIFSPEDDL
                     ILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDIESFLESSEIGASL
                     DFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYFIPKSEKFFLGKDR
                     NNVELWFEEV"
BASE COUNT         1955 a         1320 c         1150 g         1796 t
ORIGIN      
        1 tttcacaagc ttatccagat gattatcttt tactcgttat ggacaatgct atatggcata
       61 aatcaagtac cttaaagatt ccgactaata ttggctttgc atttattcct ccatacacac
      121 cagagatgaa ccccattgaa caagtgtgga aagagattcg taaacgtgga tttaagaata
      181 aagcctttta aactttggaa gatgtcatga atcaactcca agatgttata caaggactgg
      241 agaaggaggt gataaagtcc atcgttaatc ggagatggac tagaatgctt tttgaaagca
      301 gatgagtata aaaagaaagt cctcatttca atagaaatca cgactttctg atggatttat
      361 agtaaaatga aataagaaca ggacaaatcg atcaggacag taaaatcgat ttctaacaat
      421 gttttagaag cagaggtgta ctattctagt ttcaatctac tatatttttg gagtgataga
      481 aaagcccttc ataagccagt ctacttgttc tggtgtgaga gctttgacat ccttttctgt
      541 acttagccaa atcaatctgc cgttctcaaa gcgtttatat agtagccaaa atccttgacc
      601 atcccagtaa aggactttaa agcggtcttt acgtccacca caaaagagaa agacttgacc
      661 ggagaaagga tccaattcaa agtgggtttt aaccagataa gccagtgaat cgattccttg
      721 tctcatatca gttttcccac acacgagata gacctcccct aggctagata gatgaattgt
      781 catagagcaa caccttatcc aaaagctgtt ctaccatttc gagattgaga gattgaaaaa
      841 aacttacttc aagcttgcca atacgaattt taagcagaat atcatttctc tttttagaat
      901 caaaacgacg agattgtgga atctctacag gaacaatagg ttgtggcatt aaaatatcct
      961 ccaatagttt tactttacta atagtataca ggaggagctc catgaatgat agataccttg
     1021 ttatgacgcg cttaccatat tagaaagttg gctctctaat taaattaatt ctcattatat
     1081 atatcaaaaa accgcagctt gaaaactacg gtctaatttt ttgtccaaat gtaaaccact
     1141 tggaactatt attcaattta ttctttttag caaatgttcc cgttttaatc ccagttacag
     1201 tttttaatcc tctgtaaccg ttgattttac aaagctttta atttttttgc gattattccc
     1261 actcaactgt tgcaggtggt ttacttgtaa tatcgtagac gatacggtta acatgatcca
     1321 cttcatttac gatacgtact gagatttttt gaagtacttc ccatggaatt ttggcaaaat
     1381 cagcagtcat accatcgata gaagtgatag cacggattgc aatcgtgtag tcatacgtac
     1441 gaccgtcacc cataacaccg actgagcgaa cgcctgtgtt aacagtgaag tattgccaaa
     1501 tatcgcggtc aagtccagct ttagcgattt cttcacgaag aatagcgtct gattcacgaa
     1561 cggtttcaag tttctcttca gtgatttcac ccatgacacg gatagcaagt cctggtcctg
     1621 ggaatggttg gcgccatacg atgtggtctg gcataccaag ctctgtacca agagcacgaa
     1681 cttcatcctt gtaaagagta ttgagtggtt caatcagttc aaactgcata tcttctggaa
     1741 gaccacccac gttgtggtgt gacttgatag tttgagctgt atccgtacca gactcgataa
     1801 catctgtata taaagtacct tgagcaagga atttcacatc tttgagcttg cttgcttcgt
     1861 catcgaatac atagacaaac tcgttaccga tgattttacg tttttgttca ggatcagaaa
     1921 cgccagcaag tttgtcaagg aaacgtttag cagcgtctgc tttgacgata ttcaaaccaa
     1981 acttaccacc gagcatgtcc ataacttgat cagcttcgcc tttacgaaga agaccgtggt
     2041 ctacgaagat acagatcaat tgatcgccaa tcgctttttg gagaagaacc ccaacgacag
     2101 atgagtcaac accacctgat agaccaagaa ggacacgttt atcaccgacg gtttcacgaa
     2161 tttttttgat ctgcatatca atgaaattat ccattgacca gtcaccttta gccttacaaa
     2221 tgttaagggc aaagttacga aggatatcat ttccgtatac agaatgacga acttctgggt
     2281 ggaattggat accgtaaatg tgtttatctg ggttttcgat ggctgcgtat gggcagtcag
     2341 ctgatgtacc tgtacgaaca aagtcagcag gaatctcagt aaccgcatca ccatggctca
     2401 tcaaaacagt ctgttcatca ggtgttgatt caaaaagcgc tgatggtgtg tgagttaggg
     2461 ttgattgacc gtattcacga tttccagcat cacctgcagg aacaactttt cctccaagtt
     2521 tatgggtcaa taactgcata ccataacaga ttcccaaaat tggaattcct agttcgaaga
     2581 tttctgggtc aatgtcaaat gaaccatctt catatacaga atttggacca cctgatagaa
     2641 taattcctac aggattgact tcacgaactt cagcagctga aattttatgg ctttttagtt
     2701 ctgaaaaaac accaatctca cggatacggc gtgaaatcag ctggttgtac tggctaccat
     2761 agtccaatac gatgattttt tctacatctt gcaaatcagt tgaaatgttg ctcatctttt
     2821 tcctttctca aaagaaatat cttttctcaa tattctatca caaataaggg cgaattacca
     2881 actaaacaag gcgaagtttg actttttcta gtttattggg gtacaataga gaaaagatag
     2941 aaatgaagtg aaaaatatgc tacctgctta tatgaaaatc catgatcaga taaaaaagga
     3001 tattgacgag caccgttggg ctattggtga gaggcttccc agtgaaagag atttagctga
     3061 gcagtttgcg gtcagtcgca tgaccctccg ccaagccgta tctctattag tcgaagaagg
     3121 cgtcttagag cgccgtgtag gaagcggcac ctttgtttcc agtactcgag tacaagaaaa
     3181 gatgcgaggg acaaccagtt ttactgaaat tgtcaaatcc caaggtaaag ttccctctag
     3241 ccagctcatt tcctacaaaa aaaccattcc caatgagcaa gaagttgcca agctaggaat
     3301 ttttccaacg gacaatatta tccgaatgga gcgggtccgc tatgccgacc aagttcccct
     3361 agtttatgaa gttgcttcta ttcctgaaaa attcattaag gactttaaaa aagaagaaat
     3421 caccagtcat ttcttccaaa ccttgcaaaa atatggctat cgtatcggca aatctcaaca
     3481 gaccatctat gctcgccttg ctaaagaaaa gattgcccac tatttggaag ttgaaaaagg
     3541 acatgctatt cttggattga cacaggtttc ctacctagaa gatggtactg cttttgaata
     3601 cgtaaaaagt caatatgtag gcgaacgctt tgaattttat cttgaaaata attagattac
     3661 cacgcaaaaa ctctctgtca cagacaaaga gtctttttta tttttctaag aataaatctt
     3721 tcaaagaaat caaagttaca gcaaacaata tcattgccat accaataaaa tcaatgggat
     3781 aaaattgttc attcattatt aagaaggcaa agaaaaccgc cgatattggc tcaattgaag
     3841 ccaacaagct tgacttgacc ggtcctatca gactggctcc tttaaggaaa gctgtatagg
     3901 caaagacagt cccgataagg ataatgcctg caaacgcaag gagaaaatca agactagtcg
     3961 ggatatcggc ctgtagaacc cctgtaaaag gaagggcgac caaacctgct atgaccattc
     4021 ccacaccaat gaccaagctg ctcccccact ttttaatcaa ggctatgggt aaaatgatat
     4081 acagagcata agtcaaggca gaaaagagac cccagaacag gccagcaggt gtcatggata
     4141 actggtccaa ctgcccatgt gttgcgatca ggaaggttcc tccgatggcg aatatgatgg
     4201 aaactatctc tcccagtgtc ggtgccaccc tatccttgat acagctataa attaaaattc
     4261 cgacaggaca aacatactga agcaccgtcg ctgttcccgc attggtctcc tgaatagcag
     4321 acagataggc gaattggttg agaaaaagac caatcagagc aaaaataaga agagacagca
     4381 aactctttct atcctttaaa aagaccagta ttttatcctt tgcagtagca taagccaaga
     4441 gcatgagaat tccaccagcg attaaaagac gcaagttggt taagaccaga gccgaaattc
     4501 cgtgtgccat caggtattgg ccactcgttc ctgacaaccc ccaagcaata ccagccacaa
     4561 ctgttagtaa agtccctttt aaactatttg acatgtctct ccttactctt ctttgcgaat
     4621 caagttcatc acttgattgc ggtctaatat ccattgagct ctctcgtcct tttcataggt
     4681 acgattcgat aggaaaatag tggcttcttg cttctgacga ttccacatga taaaggtacc
     4741 tgtatagccc gtatggtcta gccaatctcc ttccaaattc catgctaaag aacgttcctt
     4801 gtcatccaaa ggagaaaaat tttgatttaa gtctcttgca aaatcatctg ctaaatagtg
     4861 ttctaaaaag atttgtaaat cctttatagt cgaaaataaa ccagcactcc cagcatgtct
     4921 acccaggaga cgagccttgg gatcatgcac tatgcctgcc tctacacctc taactgttgg
     4981 aacagcaagc tcaactggcc caaacttggt ttccgtcatt ccccaaggtt tccagacttg
     5041 atcctttaaa atcacatcca aatcttgatt aaaaattctt tccaaaataa agcccaacag
     5101 caaaaaatgg acatccgaat aaagaaaggc tggctgactt cgtctgttga gatgaaacat
     5161 cgcttccttt aattcagggg ctgttaaaag atcacgatta ggaataaaag gatcaaggtc
     5221 tgttgcatga gtcaagagct ggcgaatagt gatgtctgga taatcactct caggtaaaaa
     5281 atctattacc agtctatcaa tatctaattg acctatttcc cacaagaagg tacaaactgt
     5341 gccaacccca acaaccttgc tgacactagc taggtcataa actagtcctg cctcagtctg
     5401 caagccatgc tctgggtcac tctggcctaa atagaactct gtccattgat tgtccttaaa
     5461 atacgcaaaa gaggctccgg gataaattcc tgcctcgatt tgttcttcta tttttttaat
     5521 aatcttggtc cacttcatac ttcttcaaac cacaattcaa cattatttct atctttacca
     5581 aggaagaatt tttcagactt aggaataaaa tattcagtag actcaaactt ctggcgaaga
     5641 cttgctatgt ctaattcatt gaccaagaac ttgagcatag ataagtccca ggtaaccgta
     5701 ttgtccacag tcaaatcctg cccctgagct ggaataaaat caagggatgc tccaatttca
     5761 gatgattcca agaaactttc gatatcagtt gggagatgta attccataga aatctcaaat
     5821 ttacttaaag aaattgatgc caaatctgtt tgaaattcag gcttttctcc tacttctact
     5881 agacttgctc tgtcatcttc cgcatgaatc aaaatcaaat catcttctgg tgagaaaatt
     5941 tcaaaagcgt agccattttg acctttatat aatcgatgaa tcgaatctgt tttagataag
     6001 attccttcaa tttctaaggg attttccacc ttgacaatca atctagctag tttttttctt
     6061 ccctctacct tacgagtacg catactggga gcttcttcta aaaccagctt ttcaagaccc
     6121 gtttggtcac ctagtgacag aaaggccgat tcttctaaca aggccttcat tccaagggtt
     6181 tcaatataaa atgtttcatt taattttcta ttattagctt t
//
LOCUS       DAVKGC010000108         6144 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKGC010000108 DAVKGC010000000
VERSION     DAVKGC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.108"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(102..377)
                     /locus_tag="VW049_001763"
     CDS             complement(102..377)
                     /locus_tag="VW049_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9432839.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(484..1323)
                     /locus_tag="VW049_001764"
     CDS             complement(484..1323)
                     /locus_tag="VW049_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9432840.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1465..2313
                     /locus_tag="VW049_001765"
     CDS             1465..2313
                     /locus_tag="VW049_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9432841.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQSYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2406..3323)
                     /locus_tag="VW049_001766"
     CDS             complement(2406..3323)
                     /locus_tag="VW049_001766"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9432842.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3760..3965
                     /locus_tag="VW049_001767"
                     /pseudo
     CDS             3760..3965
                     /locus_tag="VW049_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4276..4569)
                     /gene="rpmA"
                     /locus_tag="VW049_001768"
     CDS             complement(4276..4569)
                     /gene="rpmA"
                     /locus_tag="VW049_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9432843.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4586..4930)
                     /locus_tag="VW049_001769"
     CDS             complement(4586..4930)
                     /locus_tag="VW049_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9432844.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4946..5260)
                     /gene="rplU"
                     /locus_tag="VW049_001770"
     CDS             complement(4946..5260)
                     /gene="rplU"
                     /locus_tag="VW049_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9432845.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5296..5383)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5442..6134)
                     /locus_tag="VW049_001771"
     CDS             complement(5442..6134)
                     /locus_tag="VW049_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9432846.1"
                     /translation="MRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLVAGQT
                     GNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIPLAIL
                     SIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDY
                     FRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLSMLFA
                     SWQKKVKEKASF"
BASE COUNT         1887 a         1266 c         1031 g         1960 t
ORIGIN      
        1 gaacattcaa ctttttaact ctatttatta ctagatttca taattaaaaa gcctactgac
       61 caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac agcgtcttta
      121 agagctttac cagctttgaa tgctggcact ttagaagctg caattgtcat ttctttacca
      181 gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt accaaaaccg
      241 atcaactgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa tacagcttca
      301 actgctgctg ctgagtcttt cttagtcaat tctgtagctt ctgctacttt agcgatcaaa
      361 tcttgtttgt ttgccatgtt aatgattcct ccaaataatt tctaattaac aaatataatc
      421 atatcctaaa atccctttta ggtcaagtta aaaacgctat ttcttcccat tttatttatt
      481 tttttaggag tggtaacgta tcaaaatagc ccaagcgttc tcacccgtgt gagtttgaat
      541 aatggaaccc gtttccaaaa cagaaattgg cttttcaaca taagcttgta agctttcttt
      601 catctctttt gcccaatcat cactaccaga atatgaaatt ccaatctctg ctacagcacg
      661 ttcagaaagc gatgttatca actcatctaa ccatttttta aatgttttag ttccacgacc
      721 tttaaccatt ggctgcaatt catggtcttt catttgcatg acaacacgga tattgagaag
      781 agagctcaac aagccagtta cacggctaat tcgtccacct ttgacaagat tttccaaagt
      841 tgaaacacca atataaagct ctgtatggtt tttaacctct tctacatgag ataaaattgc
      901 ctccatatct ttaccttctt gagctaactt cgcagcctca acaacttgga atttcagggc
      961 ttggtcagtg aaggaactat caacaacagt cacatctgca gtagataggc tagcaccttg
     1021 gcgtgctgct tctaccgtac ccgaaagagc atgggacata tgaatagcaa gaatctggcc
     1081 accatctttg cataggtctt caaaaatctc agcaaagaca cctacaggtg gctgacttgt
     1141 tttcggaaga ttcttacttt cttgcatcaa ctgaagaaat ttaccttctt ctttcaaatc
     1201 cgcatcagaa taaacaacat tatcaatcat tacagataat ggaacaattg taatatctaa
     1261 ttgctttact agttcaggtt caatagtaac agatgaatcg gttacaatct taatttttgt
     1321 catagtatca atctttctat tttaggattc agattggttt ccttacttct aattatatca
     1381 aaaaaaagat taaaaatcct aatggagtca atcaaatttt ccgtaaaatt tgatataatc
     1441 aacttataag aaaagaggtg tcctatgatt aaaaaaattt accccatttt taccatttta
     1501 ctaggtgctg ctatttatgc ttttggactg acttattttg tagttcccca tcatctcttt
     1561 gaaggagggg cgacaggcat taccctcatc accttttatc tttttaaaat ccctgtttcc
     1621 ctcatgaacc tgctgattaa tattcccctt ttcatcctag cttggaagat ctttggagcc
     1681 aaatccctct attctagttt actaggaacc ttagctttgt ccgcctggtt agcttttttt
     1741 gagcatattc cccttcatat tgatcttcaa ggtgatttac taatcacagc ccttatagcg
     1801 ggaatcctat tgggaattgg ccttggaatt atttttaatg ctggaggtac aactggcgga
     1861 actgatattc tagctcgtat tctcaacaaa tacactcata tatccatagg aaaactgctc
     1921 tttatcttag atttttgtat tctcatgttg attctcctaa tcttcaagga tttgagattg
     1981 gtttcctaca cgcttttgtt tgattttatt gtttctcgtg ttattgattt gattggtgaa
     2041 ggaggatatg ccggcaaagg ctttatgatt atcacaaaac gtcctgacca acttgctaag
     2101 gcgattaatg atgacctcgg aagaggtgtt acttttattt ctggtcaaag ctactatagt
     2161 aaagaaaatt tgaaaatcat ctactgtatt gtcggaagaa atgaaattgt gaaaacgaag
     2221 gaaatgattc atcgaatcga tcctcaagcc tttataacta ttacagaagc ccatgaaatc
     2281 ctaggagaag gcttcacctt tgaaaaagaa taaaaagagg taatgtcgtg acctcaaaag
     2341 ttagactaaa tcatctatct tttgggttac agacaacctc ttttttgttt tatttactca
     2401 agctcttaag accaattccg agttacttct tcatcagcct ttaactgatc cactaattgg
     2461 tcaactgagt caaatttggt catatcacga atgcgatcaa gccaataaac catgacggtt
     2521 tccccataaa tatcttgatt aaaatcaaaa atattgactt caaaacgtgc ttcttctcca
     2581 tcaaaggtca catttttccc gacactagcc atagcacgat acttctgtct ttgaatctca
     2641 acatcaacga cataaacgcc atctgctggc atataagtac ggtctaaaag cactaaattc
     2701 gctgtcggat aaccaattgt acgaccacga gcattaccat gaaccaccat acctcttgat
     2761 ggaagcggtg cccccaaaag ttttcctgct tctttcacat ttccatctaa aatagcttga
     2821 cggatacgag ttgaactaat ctttcctttc tcatcttcta caggtggaac aatgataact
     2881 tctccatcaa agtaatcctt taaatcttct gctgtttttt tgtcagaacc aaatgtataa
     2941 tcaaaacctg caacaataat tttggcattc atagccttga tataagttgc aaagaattct
     3001 tgtgcagtga gactagcgaa ttgactacta aaatcaagga gatataattc ttctacacct
     3061 tcgcgcttta attttctttc acgttcagca gggttcaaaa tatgcaaaaa caaatctgga
     3121 tgataaggct ctaaagcgat ctttggagat tcattaaagg tcataacgac gataggcaat
     3181 aaatcctttc tcgcagcctt gttggcaaca cgaaataatt cttgatgccc cttatgtatg
     3241 ccatcaaaat agccgagaac aacgactgaa tcagatggtg tgccaatatc tttttggttt
     3301 tttataggaa tagtaataat cataaaataa ttatatcata gcgatagcta tttctggaac
     3361 agaaaatctg aaatgttgtt tttttcacat gaagtgtacc tgttttcaaa aagcacttta
     3421 ttctatcgtt gcttaactat gaactttgca atattcttct caaaaacttg taggacatct
     3481 tcaaaatttt gcaaggagtg attagacttg ttcggtaacc ataaagtgtc atactatgtt
     3541 atgtatgaaa aagcaatgca actaactcct gagaacttta aattactaat tggtgccgaa
     3601 aaggtagaat ttagaatcga ggtacaccta tggctgtaaa atttacaaaa tgagacaact
     3661 tgggcaagat gtttgaagaa tttcctaaac tccctgattt gaagcaagtc actttccctg
     3721 atgacaaaga aaaaagccaa aacagcaaag aaaaactaga tgactgcttt ccaacaactc
     3781 ccatctagtg tgcttcagac tgggctattt ttctctccat ctgttagctt ggattctcag
     3841 accgtttcag ctaaagaata tcttttccct tatcagaagg aacggctcaa accattcaga
     3901 caagtgaagg gacgacaagc caatatttga aaccagatag cagttcttat agtcaattga
     3961 aataaaatct gaagaaatcg agtaggaaac tcatatcaat ttttaacagt gttctattcc
     4021 agattcatac tcaatgaaaa ttaaagtgca aactaggaag ttagccgcag gctgtacttg
     4081 agtacggcaa ggtgaaattg acgtggtttg aatttgattt tcgaagagta tcaatccact
     4141 atacatatta aaaagtatgg gtatcaaaat aaaaaaacag gctctccgaa aactcggaaa
     4201 gccttatttt aagctgtttc tagcttcctc acctttacat ttcaaggttc gggataaaag
     4261 gttcaatgaa cctttttatt ttgcgattgg gtaaacagaa acttgttttt tatcgcgtcc
     4321 tttacgttca aagcgtacta cgccttcaac tttagcgaac aaagtatcgt ctccaccacg
     4381 accaacgttt acacctggat agatgtgtgt accacgttga cggtaaagga ttgatccacc
     4441 tgttacagtt tgtccgtcag ctgctttagc tccaagacgt tttgcttgtg aatcacgtcc
     4501 gtttgatgta gaacctccac cttttttgtg ggcgaaaagt tgcaagttgt taagagtcat
     4561 ttttaacata atgttttcct ccgtgttagt tttctgtgat aactctggtt tggacgaact
     4621 cagaagagtt ctccgataag tttgccatac ctaagaaaaa tgattcaaag aataactggg
     4681 tcatttctct ctggtgtgaa ggaagatctt ttggtatttc aaccatcaga tagccacctt
     4741 catcttcgtt taattctagg attggttcat agcctgcaaa tttctcaata gaattgataa
     4801 agttaatggc aagcgtagaa accgatgcac acacgacatc taagccgtat tcgccactct
     4861 cggcgtgtcc agtaatttcc gcactcctca gctcgccatc ttcggctctc tcaaagactg
     4921 cctgtatcat gtgttctcct taaaattaag cgttgattgc gttgatgaca acttttgtat
     4981 atggttgacg gtgaccttgt ttacggtggc tacctttttt aggtttgtac ttgtaagtaa
     5041 ccactttctt ttgttttcct tgtttttcaa cagttccaac tacagtagct ccagcaacaa
     5101 gtggagttcc gacaacagtg ttttcaccac caacaagaac aacttcgtta aaagtaactt
     5161 cttgaccagc ttcaacgttc aatttttcaa cgtaaactgc ttgaccaact tcaactttaa
     5221 cttgttttcc gccagttttg ataattgcgt atgtgctcat tatgcacctc ctatgatttt
     5281 tatgggtttc cccgattttt tgtgaagact cgcctagcat cgtgggacga accacttaaa
     5341 cttgaataat caagcaacga tgttcgtgcg gttgcacagg atcgtgcata gtcaactctt
     5401 caagtatagc atatctccta ttttcttaca agtaataaca cctaaaatga agctttttct
     5461 tttacttttt tctgccaaga ggcaaaaagc atgctgaggt aaaaaacgct catcataata
     5521 ggaacaccaa gaatggtctt ttcatgatag aaaatcgtca aataggctga aaagacaacg
     5581 ccaaggacaa aactactaag caggctaaca aatatgaatc cttcacgcaa aaaaggagtg
     5641 tgcttggttc ggaaataatc tccaaaagcc agcatggtcc gtttgatatt ccctgtcata
     5701 aaagcgttat tataggcaat acccgacact tctccaaaag cagttgtcac cagtcccata
     5761 cagaaggcca agggcggcac tagatagata ttatccacag tttgcggcac aaaagcaata
     5821 atgattgata agattgccaa gggaatcaag gacagaatag gttttttcac aattctcaat
     5881 ttttccttat aaatcgttaa taaaaagact cccatcataa acgctagcaa ggtgagaacc
     5941 ttgtccctaa catccgaaac attattttta attaattcta ctgaaagaaa gacaacattt
     6001 ccagtttgtc cagctacaag ggtattcccg cgaacaataa aagtgtaagc atccacatat
     6061 ccagcacaaa acgtcaaaaa aagtgctaac cttttagact gacgtgatat ttttcttata
     6121 ggtaataacc tcattttacc tccc
//
LOCUS       DAVKGC010000109         6072 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKGC010000109 DAVKGC010000000
VERSION     DAVKGC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.109"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            159..1928
                     /locus_tag="VW049_001772"
     CDS             159..1928
                     /locus_tag="VW049_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEU9432847.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLGSPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            1952..3907
                     /locus_tag="VW049_001773"
     CDS             1952..3907
                     /locus_tag="VW049_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9432848.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRAIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            3909..4346
                     /locus_tag="VW049_001774"
     CDS             3909..4346
                     /locus_tag="VW049_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9432849.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPIGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4408..5544
                     /locus_tag="VW049_001775"
     CDS             4408..5544
                     /locus_tag="VW049_001775"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEU9432850.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIADEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT         1859 a         1022 c         1244 g         1947 t
ORIGIN      
        1 agttcatgta cacctgatat gatgcgtttt ataattttta agcctttttg cccagcctcg
       61 tcaaaagtaa tgttttgaca caaaatctgt gacaaaactt tagttttaaa ggtttttaac
      121 tttgtatata ctagttttaa gaaaaggagg atgatctaat ggaagaaaaa gtatcattga
      181 aagtcagggt tcaaaaacta gggacatcgc tttcaaatat ggttatgccc aatattggag
      241 catttattgc ttggggagta ttgactgccc tctttatcgc tgatggctat ctgccaaatg
      301 aacagttagc tactgttgtt ggtcctatgt taacgtattt attgccaatc ctgattggtt
      361 acacaggtgg atatatgatc catggccaac gtggtgccgt tgtaggagct attgctactg
      421 ttggtgcaat tacaggttct agtgttccta tgtttatcgg agctatggta atgggcccac
      481 tgggaggatg gactatcaag aaatttgatg agaagttcca agaaaaaatt cgtcccggat
      541 ttgaaatgtt agttaataac ttctcagctg gtctcgttgg ttttgcatta ttgcttttgg
      601 ctttctacgc aatcggtcca gtcgtatcga ctcttactgg agctgttggg aatggtgttg
      661 aggctattgt caatgctcgc ctccttccta tggctaatat tatcatcgaa ccggctaaag
      721 tccttttcct caataatgcc ctcaatcatg gcatttttac tcctctggga gtagaacagg
      781 tagctcaagc tggtaagtca attctcttcc tattggaagc taatcctgga ccaggcctgg
      841 gaattctatt agcttatgct gtattcggta aaggttctgc taaatcttct tcttgggggg
      901 caatggttat tcatttcttc ggagggattc atgaaattta ctttccttat gttatgatga
      961 agcctactct atttttagct gctatggcag gaggtatctc tggaactttt acttttcaac
     1021 tcttagacgc tggtcttaaa tctccagctt caccaggttc tattattgcg attatggcta
     1081 cggcgccaaa aggtgtttgg ccccatctaa atgttctttt aggtgtttta gtggcagcag
     1141 ttgtttcttt ccttgtagca gcccttattc ttcatgcaga caagtcaact gaggattcgc
     1201 tcgaagctgc tcaggcggct acccaagcag ctaaggctca gtctaaaggt cagttagtat
     1261 caacttctgt tgatgcagtt gtttcgacag actcagtgga aaaaatcatt ttcgcctgcg
     1321 atgctggtat gggaagctct gctatgggag ctagtattct tcgagataag gttaaaaaag
     1381 caggtctaga gattccagta tctaatcagg caatctcaaa tttgcttgat acaccaaaaa
     1441 cattaattgt tactcaggaa gaactgacac caagagctaa agacaagagt ccaagtgcta
     1501 ttcatgtttc tgttgataat ttcttagggt cccctcgtta tgatgaaatt gtagcttcat
     1561 taacaggagc ttctccaata gcagaaattg aaggagatat accaacttca gcaccagtag
     1621 atagtcagga aattgacctt aaccatattg atgctgtagt agttgcttat ggtaaagcac
     1681 agggaactgc aactatgggc tgtgaaacga ttcgggctat ctttagaaac aagaatattc
     1741 gtattccagt ttctactgcc aaaatttcag aattaggtga atttaattct aaaaacataa
     1801 tgattgtaac aactatttct ttacaggcag aagtgcagca agcagcaccg aattctcaat
     1861 ttcttattgt ggatagttta gtaacaacac cagaatatga caaaatggct gctagaatgt
     1921 acaaatagaa ctagaggttt ctaaattacg aatgctatta accaaacgag aagaacaatt
     1981 attgaaggct ttcctacatg tagggaagct ttcaatgcaa gatatgactg aaatcttaca
     2041 ggtttcatct agaacaattt atcgaacttt atcagatttg acagatagca tggagcaata
     2101 tggaatcgaa ataacgaagc atgggaaata ctatattttg actggagagt tggatgattt
     2161 gccgacagaa cttgaagtgt tagttgagta tagtccccaa gaaagacaag agttgattac
     2221 ctatcgcctt ctgactgaga gtggttttgt caccaatgaa gcattgcaag agtgcacgaa
     2281 agtcagtaat gtaactatta ttcaggatat ttcagatatt gataagcgtc ttttagactt
     2341 tgatctgaaa attgaacgac aaaaaggtta tcggatttct ggtgattcag ttggtaagag
     2401 aagatttttg gctattttac tgacaaactg tatctcagta gcagattttt caaccggtaa
     2461 ttttgggagc tttgatattt tagaagcaga tagaactagg ctggccagtc agattgttaa
     2521 taagcaactg tcaggttttc cagatatgga tgctaggatg aagatgtttt ttgcgatctt
     2581 gttatctctt ataggtcagg agcaaaacat tgaaaattca cctaatacta gtaagcaggc
     2641 tttggaaatt tctcaaaaaa tttttcaagc ttactctaag cagactgcac aattttatag
     2701 tattcaggaa attatctatt ttgcgagcat cttggatgaa ttaatcatta aacgtcagga
     2761 caatccgctc tttacggaga aatttgatgg tgaatttttc tacaatattt caaatctgat
     2821 tgatacggtt tccatgtata ccaagattga cttttttaag gacaaggttt tattcaattt
     2881 tcttttccat catattcggc tcagtttagg cgtccctatc ctttttcagg gtgaaaattt
     2941 gccagaatct gtccagattt tagttgaaag gaataaattt ctttatacag tcatcagtct
     3001 tttagtgaat gatatttttc cgaaatatct tcatacagag tatgagtatg gcatgattgc
     3061 cctacatttt atctctagct taggccgtag tccagagatt tatccagtcc gtgttttgct
     3121 tttaacggat gaacgtcggg tcactagaga tttattagtc agtaaaatta agagtgttgc
     3181 tccttttgta gagttgatag atattcagtc tctagtagat taccacagta ttgatctcag
     3241 tcagtatgat tatattttat ctaccaagcc gctgactaat caggaaatcg atgtaatttc
     3301 tagttttcca accgtcaaag aattgcttga attacaggaa cgacttcagt atgtacaggc
     3361 acatcgtgca attgtcgcgc gtgatgctat cgctccagag aaaagttatg acttgcaaga
     3421 ttatttaata tctagtagtc agcttttgag tcaattcgag ttggttcaat tggagaataa
     3481 tcaatcattt gagcacacgg tagaacaaat catccaatat cagaagaatg tgagtgacag
     3541 agattaccta acaagaaaat tgttatctca cttccagaat agtcctatgg ctattcctaa
     3601 tactggtctg gtgcttttac atagtcagtc tagcaaagta acaacaaata gttttactat
     3661 gtttgaactc aaactaccta tctccgcatt gtcaatgaaa cgagaggaag aagaggtcaa
     3721 aaggtgtctg ctaatgctaa tgtctaaaga agctagcgag gaagcgagag atttaatgac
     3781 agctattagt cagtcgatta ttgaaaatca tctttataca gagatttaca agacgggaaa
     3841 tcaatccatt atttatcaga tgctaaatac tatttttaac gaaaaaatta agaaattgga
     3901 gaactaatat gaaacttgaa aaacatttga ttaagcttaa taaacaattt tctaacaagg
     3961 aggaagctat ttgttattgt gggcaagttc tttatgaggg tggatatgtt aatgaagact
     4021 atattgaagc catgattgag cgagataaag agctatctgt ttacatgggt aactttatcg
     4081 ccataccgca tggaacagat gcagcaaaaa aggatgtcct caagtctggt attacagtcg
     4141 ttcaagtccc tataggggtt gattttggga atgtatctaa ccctcaagtg gcaacggttc
     4201 tttttggtat tgctggtatt ggtaatgaac acttagaaat tattcagaaa atttctatct
     4261 tctgtgcaga tgtagataat gttcttaaac tagcagatgc tcagtcaaaa gaggaagtat
     4321 tgcgcttatt tgatgctgtt gaataattga atttcgtcat ttgtcatcta gtatatatgt
     4381 ccctcaaata ggaaaaggag aaattgaatg aaacattctg ttcattttgg tgccggtaat
     4441 atcggtcgtg gttttatagg tgaaattcta tttaaaaatg gtttccatat tgattttgtg
     4501 gatgtcaata atcagataat tcatgctcta aatgaaaagg gcaagtatga aattgaaatt
     4561 gcacagaaag gacagtctcg tatagaagta actaatgtgg ctggcattaa tagcaaagaa
     4621 catcctgagc aagtcattga agcgattcaa aagacggata ttattactac tgcaatcgga
     4681 cctaatatac tcccttttat cgccgaactt ctagccaaag gaatcgaagc tcgccgagtt
     4741 gcaggaaata cacaggcatt ggatgttatg gcctgtgaaa atatgattgg cgggtctcaa
     4801 tttctttatc aagaagtcaa gaaatattta agtccggaag gtttgacatt tgctgataac
     4861 tacataggtt ttccaaatgc tgcagtagac aggattgttc cagcacaaag tcacgaagat
     4921 tccctttttg ttgtggtcga gccctttaat gaatgggtcg tggaaaccaa gcgtcttaaa
     4981 aatccagatt tacgtctaaa agatgtgcat tatgaagaag atttagaacc ctttattgag
     5041 cgaaaacttt tttcagtcaa ttctggacat gcaacttcag cttacattgg tgcgcattat
     5101 ggtgccaaga caattttgga agctcttcaa aatcctaata tcaaatctcg gattgaatct
     5161 gtattagctg aaattcggag tctcttgatt gccaaatgga actttgataa aaaagaattg
     5221 gagaattatc acaaagtcat tatagaacgg tttgaaaacc ctttcatagc ggacgaggtt
     5281 agtcgcgtag ctcgcactcc aatccgaaaa ttaggctata atgaacgatt catccggccg
     5341 atacgtgaat tgaaagaact cagtttgtca tataaaaacc tacttaaaac agttggctat
     5401 gcctttgact atcgcgatgt aaatgatgaa gaaagtattc gattaggtga attgttggct
     5461 aaacaatcag tcaaagatgt tgttatacaa gttacaggtt tagacgacca agaattgatt
     5521 gagcaaattg tagagtatat ttaatctttt tcgaaaatct cttcaaatca ggttagcatc
     5581 gctttgtctt aggcatatgt tactgacttc gtcagttcta tctacaacct caaagcagtg
     5641 ctttgagctg actccgtcag tcttatctgc aacctcaaaa cactgtttga gttatctgcg
     5701 gtaatctttc tagcttgtct ttgatttttg ttgttattta taaggtaaaa gaagctggac
     5761 aaaaagtctt caaaatcgga aaaaggcagc ctatcgggtg ttcaaaaatc ttgataggat
     5821 gtcctttatt atggaaagcc ttattggatt ttctcctcag attgagtttt tgatcagctt
     5881 tatgagatag gttttgctag agatgtagcc catcatgtta tttttatgga cagtgggaaa
     5941 attgttgaaa aaaataatgc ccatcaattc tttagtcgtc caagagaaga acgaaccaag
     6001 caattttgga acgaattctt tcgaatgcga tctatatagt aaaatgaaac aagaacagga
     6061 caaatcgatc gg
//
LOCUS       DAVKGC010000110         6034 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKGC010000110 DAVKGC010000000
VERSION     DAVKGC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.110"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            142..909
                     /locus_tag="VW049_001776"
     CDS             142..909
                     /locus_tag="VW049_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9432851.1"
                     /translation="MIGISLIPALYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKPTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNAILTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            994..1674
                     /locus_tag="VW049_001777"
     CDS             994..1674
                     /locus_tag="VW049_001777"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9432852.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVLVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
     gene            complement(1775..4327)
                     /locus_tag="VW049_001778"
     CDS             complement(1775..4327)
                     /locus_tag="VW049_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9432853.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIRFHVKLIYIIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFFFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4389..6011)
                     /locus_tag="VW049_001779"
     CDS             complement(4389..6011)
                     /locus_tag="VW049_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9432854.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         2027 a          990 c         1108 g         1909 t
ORIGIN      
        1 aaatagattt tttatcttgt aatttagatt ttttaatgta taatctatta tatcaaaatt
       61 ttagacaata tgtttaaaaa aggagaaact aagtttaaag aatggaaagc aatttaaaaa
      121 aaaccaacct ttattattgt catgatcggg atttctctta ttccagctct atacaatatc
      181 atatttttgt catcaatgtg ggatccatat gggcaattgt ctgacttacc tgtggcagtt
      241 gtaaataatg ataaagaggc ttcctataat ggtaatacta tggcaatagg aaaagacatg
      301 gtgtccaatt taaaagaaaa taaaaccttg gattttcatt ttgtagatga agaggaagga
      361 aagaagggat tggaagatgg cgattactat atggtagtga ctttaccaag tgatttatct
      421 gaaaaaccaa ctacattatc caatattcaa tcgacagcag cttatcaatc attgacaagt
      481 gagcaacaaa ctgagataag tgattctgta tctcaaaatt caactgatag tattcaatcg
      541 gctcagtcaa ttgtagcttt agtacaagat ttacagggaa gtttagaaaa cttacaaaat
      601 caatcttcta atctttcgac tttaaaaaat caatctaatc aagtatcacc tattacttct
      661 acttctttga taggattgtc aagtggatta acagagatac aaggagatgt tactagcaaa
      721 ttagttcctg ccagtcagtc gattgcatca ggtgtaaacg catatactac aggtgttgat
      781 aaagtttctc agggcgcaag tcaactaagt gaaaaaaatg ccatcttgac aggtagtttg
      841 gataaactag tttcaggctc aaacaccttg acacaaaaat cttctagatt gacagcagga
      901 gttggttaat tacaatcagg atctgggtaa ttagcagaca aatccagtca gttactttca
      961 ggtgcttctc cattagagaa tagagctaat aaattggcag atggatctgg gaaactagca
     1021 gaaggtggaa caaagttaac ttctggattg gaagatttac agacaggact tgcttcttta
     1081 ggacaaggac taggtaatgc tagtgatcaa ctcaaatcag tatcaacaga atctaaaaat
     1141 gcagagattt tgtcaaatcc actcaatctt tcaaaaacag acaatgatca agttcttgta
     1201 aatggaatcg caatagctcc ttatatgata tcagttgctc tttttgttgc agcaatatca
     1261 acaaatatga tatttgcgaa attgccttca ggacgtcatc cagagagccg ttgggcttgg
     1321 ttgaaatctc gagctgaaat aaatggtatt atagctgttt tggcaggaat tttggtatat
     1381 ggaggagttc agcttattgg tttaactgct aatcatgaga tgagaatatt tattctcatc
     1441 atcctaacaa gtttagtatt catgtctatg gtgaccactt taacaacgtg gaatagccgt
     1501 ataggagctt ttttctcact tattttgctt ttactacagt tagcatcaag tgcaggtact
     1561 tatccacttg ctttgacaaa tgatttcttt agatctatta atccctggtt accaatgagc
     1621 tattcagttt cgggattacg acaaacaatc tccatcaaca agtcattttc ctagctgtca
     1681 tactagctct atttactggt ttaggtatgc tagcctatca acataagaaa atagaagaag
     1741 attaaaaaaa tcgaccgatt aactggtcga ttttttatgc cttagatgac tttcgtctgt
     1801 gattatagat tccaaatagt aagagagaag taaaggaaca gattgctcca gtaataaaac
     1861 cattgggaat gaaggaaagt gtaatagttc ctttcccctt gggaatgtca actttcataa
     1921 atccagtttg agcttgttta atttctattt tcttaccatc ttggtaggca gaccaacctt
     1981 tgtcataagg aatggtgaag aaaatagatg tatcttgttg gacatcatat gtagcaaaaa
     2041 ccttgttttt agaagttgat actgtgacag gttgttcttt aattttttga attgcctcgg
     2101 tgaaagtttt ggtatctaaa cgatagaagg taggagattc aaatgatact tgtgaatttc
     2161 cagggaaact aacattgata ttgaaagttt ttttctcttt agtatatcct agattaaaga
     2221 agaagaagac attatcagtt gtaaaagtct ttttttcacc atttacaagg atgtcaacct
     2281 tcttttgttt atcgttagaa aagtgaaggt ttatgaaaga gagataaact tggctgtttt
     2341 ctggaacttc aatttgatac tggattgctg catcttcatt tgaagaactt gtgacactaa
     2401 tcaaatcatt agtattttct attttttctg ttttttcata aggtattgga gaaaaataat
     2461 caaaattgac gttagcaagt tgatttaaaa atgaggcctg attatccaag gtatgttcat
     2521 tgaacttgac atcattgtaa acagattgac tagcaactgc aatcggaaga gagtattgat
     2581 tttcatatag ggtaagatta tctttttgat agatatcttt aaagccatac ttatcaatag
     2641 gactgtctga gatattgtac tggataccaa ataaactatc agccaaaata ctattatttg
     2701 catatcggag attgagatta gtcccagagg atttaaaacc aagtttatct aaagtagagc
     2761 ttgatgaacg atttcgaaca gatgaaaatt gagagattcc attgtagttg aatttcatac
     2821 tgtcatttcc tgtctgagtt tgtagttttt cagtacgagt aaattgattt ccaatatatg
     2881 ttgagaaaga ttccatagct gggatatctc gactataagc acttcgagaa gcaaatcccc
     2941 attccttagc aattccgtcc atttgagatg aagcatttaa actcatttca accagtataa
     3001 ataaagagat tagaatggca aatagattca cagatataaa ctttttgata actgcaagga
     3061 gtaaaagaga atagacaacc aaaaattcaa gagtaagcag aatattcaaa tctgttaaaa
     3121 aagaataatg cgattttaga tagatggtag ctaaaaatcc tgttactaca agaaaaagcg
     3181 aaactaaaaa attccagact ttaagttctt tcagacgctt taagacttct gctgctgtgt
     3241 aaattaacaa ggtagagaaa atccaagcat agcgatgtaa aaacatgttt ggagtatgca
     3301 tgccttgcca aaataagtca agagcttcta tgtaaaagct tgcaattaga aatgcaaaga
     3361 atattatata tatgagtttc acgtgaaacc taatagattt cagcgtaaaa aataaaatgg
     3421 tcaaaataaa gggaagtagt ccaacaaaaa tcattgggat ggccccatac tttgttgtgt
     3481 caaaggaacc aatgaattgc ttagcaaaga gatcaagata ccagctactt tcagtttgaa
     3541 actttgtaac ttcagtcaat ttttccccat gtgtctgtaa atcaaacaga gtgggaagag
     3601 tcataatcaa actagccata ccagctaaaa aggagataac tatgaaatca agaacagatg
     3661 attttcgagt cttaaagtcc cacgaaattt gacagagata ccagaaaata agaaacaata
     3721 ctgtcatata tccaaaataa taattttgaa taaataagat tgacagactt gtaaagtaca
     3781 ataggagttt cttttcagtt atcagtagat gtaaaccagt tataattaaa ggaatcaaga
     3841 taaaaacatc tagccaggtt tttatctcta attgactgac agtgaaactc atcagagcat
     3901 aggaagtaga taaggctagt tttaaagtct gagggataga ttgaaacaat ttattcaaac
     3961 taaaaaaggt tgacagacca atcaatccaa attttaagag agtggtcaga tagacagcat
     4021 ctggcatatt cgttagatca aaaaagtaaa ctagaggcgc gagaaaacta cccaagtaat
     4081 aactagatag ggcatagaag tttagcccta gaccacttgt aaaggtgtaa aacagactac
     4141 tatttccatg taggatattt cgtaaggcta catcaaaaat aacgtattga tgaaagccat
     4201 ctcctaatag aggagagttg tcgctattcc agtagatacc ttgagataga tatactccag
     4261 acataatcac tacaggaatg atgaaagaaa taaaataggt tcgatatgtt tttaaaaatg
     4321 atttcatgtt acctcgtaga atgatagaaa actcagttgg ttaacccaac tgagttttga
     4381 agttttattt agtctttcca aagttcttta acttttgctt gtacttctgc attttctagg
     4441 aattcatcgt aggtttcatc gatacggtca atgacgccat ttttagataa gacaatgata
     4501 tggttagcca aagtttgaat aaattcgtgg tcatgactag caaagatgat tgattcttta
     4561 aagtttttca atccatcatt caagcttgag atagattcca agtccaagtg atttgttgga
     4621 tcatcaagta caaggacatt tgattttaag agcatgagtt ttgaaagcat gacacgaact
     4681 ttttctcccc ctgacaagac atttacaggt ttgttaactt catctccaga gaagagcata
     4741 cggccgagga aaccacgtag gaaagtattg tcatcttctt ctttacttgc gaattgacgc
     4801 aaccagtcaa gaattgattc tcctcctgca aaatcagctg agttatcttt tggtaggtaa
     4861 gattgactag ttgtaactcc ccacttgaca gttccttcat agtcaatatc tcccatgatt
     4921 gcacgaatta atgcagtcgt ttgaatatca ttttgtccaa taagtgctgt cttatcatct
     4981 ggacgcaaga tgaaactaat attatccaag atagtttcac catcaatctt tacagttaga
     5041 ttttctactg tcaagagatc attaccaatc tcacgttccg ctttaaagtt gataaatgga
     5101 tatttacgac tagatggtac aatctcttct agctcaatct tatcaagcat tttcttacgt
     5161 gatgttgcct gccttgactt agaagcattg gcagagaaac gagcaacgaa ttcttgcaat
     5221 tgtttaattt tttcttctgc tttagcatta cggtctgcta gcaatttagc agcaagctca
     5281 gaagattcct tccagaagtc gtagtttccg acatagagtt tgatttttcc aaagtcaagg
     5341 tcggccatgt gagtacaaac tttgtttaag aagtgacggt cgtgggatac tacgataact
     5401 gtgttatcaa agtcaattaa gaagtcttct aaccaagtaa tcgattggat atccaaaccg
     5461 ttagtaggct cgtccaagag aagaacatct ggtttaccaa aaagtgcttt ggcgaggaga
     5521 acctttactt tttcaccgtt ggccaattcg ctcatgtttt ggtagtgtaa ttcttctgga
     5581 atgtttaggt tttgaagtag ttgagaggct tcactctctg cttcccaacc tccaagttcg
     5641 gcaaactctc cttcgagttc ggcagcacga accccgtcct cgtctgagaa atcttccttc
     5701 atgtagatag catctttctc tttcatgatg ctataaagtt tttcatttcc catgataacg
     5761 acatcaatgg cacgttcatc ttcgtagtca aagtgatttt gacgaagaac agagagacgt
     5821 tcatctggac caagagagat gtgaccagta gtaggttcga tatctccagc taaaattttt
     5881 aaaaaggttg attttccggc accattagca ccgattaatc cgtaagtatt tccttctgta
     5941 aatttgatat tgacatcatc aaaaagtttg cgatcactaa aacgtagtga aacatcagat
     6001 actgtaagca atatttttct cctatatgtg taat
//
LOCUS       DAVKGC010000111         5816 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKGC010000111 DAVKGC010000000
VERSION     DAVKGC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.111"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            30..1730
                     /locus_tag="VW049_001780"
     CDS             30..1730
                     /locus_tag="VW049_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9432855.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1723..2199
                     /gene="ilvN"
                     /locus_tag="VW049_001781"
     CDS             1723..2199
                     /gene="ilvN"
                     /locus_tag="VW049_001781"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9432856.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2265..3287
                     /gene="ilvC"
                     /locus_tag="VW049_001782"
     CDS             2265..3287
                     /gene="ilvC"
                     /locus_tag="VW049_001782"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9432857.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3394..3630
                     /locus_tag="VW049_001783"
     CDS             3394..3630
                     /locus_tag="VW049_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432858.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            3868..4224
                     /locus_tag="VW049_001784"
     CDS             3868..4224
                     /locus_tag="VW049_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9432859.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            4274..5524
                     /gene="ilvA"
                     /locus_tag="VW049_001785"
     CDS             4274..5524
                     /gene="ilvA"
                     /locus_tag="VW049_001785"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9432860.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1780 a          990 c         1342 g         1704 t
ORIGIN      
        1 ttatttttaa gaggaaagaa aggggaataa tggagaaaat cagtttagaa tctcctaaga
       61 cggggtcgga cctagttttg gaaacacttc gtgatttagg agttgatacc atctttggtt
      121 atcctggtgg tgcggttttg cctttttatg atgcgatata taattttaaa ggcattcgcc
      181 acattctagg gcgccatgag caaggttgtt tgcatgaagc tgaaggctat gccaaatcaa
      241 ctggaaagtt gggtgttgcc gtcgtcacta gtggaccagg agcaacaaat gccattacag
      301 ggattgcgga tgctatgagc gatagcgttc cccttttggt ctttacaggt caggtggcgc
      361 gagcagggat tgggaaggat gcctttcagg aggcagacat cgtaggaatt accatgccaa
      421 tcactaagta caattaccaa gttcgtgaga cagctgatat tccgcgtatc attacggaag
      481 ctgtccatat cgcaactaca ggccgtccag ggccagttgt aattgaccta ccaaaagaca
      541 tatctgcttt agaaacagac ttcatttatt caccagaagt gaatttacca agttatcagc
      601 cgactcttga gccgaatgat atgcaaatca agaaaatctt gaagcaattg tccaaggcta
      661 aaaagccagt cttgttagct ggtggtggaa ttagttatgc tgaggctgct acggaactaa
      721 atgaatttgc agaacgctat caaattccag tggtaaccag tcttttggga caaggaacga
      781 ttgcaacgag tcacccactc tttcttggaa tgggaggcat gcacgggtca ttcgcagcaa
      841 atattgccat gacggaagcg gactttatga ttagtattgg ttctcgtttc gatgaccgtt
      901 tgacggggaa tcctaagact ttcgctaaga atgctaaggt tgcccacatt gatattgacc
      961 cagctgagat tggcaagatt atcagtgcag acattcctgt agttggagat gctaagaagg
     1021 ccttgcaaat gttgctagca gaaccaacag ttcacaacaa tactgaaaag tggattgaga
     1081 aagtcactaa agacaagaat cgtgttcgtt cgtatgacaa gaaagagcgt gtggttcaac
     1141 cgcaagcagt tattgaacga attggtgaat tgacgaatgg agatgccatt gtggtaacag
     1201 acgttggtca acaccaaatg tggacagctc agtattatcc ctaccaaaat gaacgtcagt
     1261 tagtgacttc aggtggtttg ggaacaatgg gctttggaat tccagcagca atcggtgcta
     1321 aaattgctaa cccagataag gaagtagtct tgtttgttgg ggatggtggt ttccaaatga
     1381 ccaaccagga gttggctatt ttgaatattt acaaggtgcc aatcaaggtg gttatgctga
     1441 acaatcattc acttggaatg gttcgccagt ggcaggaatc cttctatgaa ggcagaacat
     1501 cagagtcggt ctttgatacc cttcctgatt tccaattgat ggcgcaggct tatggtatta
     1561 aaaactataa gtttgacaat cctgagacct tggctcaaga ccttgaagtc atcactgagg
     1621 atgttcctat gctaattgag gtagatattt ctcgtaagga acaggtgtta ccaatggtac
     1681 cggctggtaa gagtaatcat gagatgttgg gggtgcagtt ccatgcgtag aatgttaaca
     1741 gcaaaactac aaaatcgttc aggagtcctc aatcgcttta caggtgtcct atctcgtcgt
     1801 caggttaata ttgaaagcat ctctgttgga gcaacagaag atccgaatgt atcgcgtatc
     1861 actattatta ttgatgttgc ttctcatgat gaagtggagc aaatcatcaa acagctcaat
     1921 cgtcagattg atgtgattcg cattcgagat attacagaca agcctcattt ggagcgcgag
     1981 gtgattttgg ttaagatgtc agcgccagct gagaagagag ctgagatttt agcgattatt
     2041 caacctttcc gtgcaacagt agtagacgta gcgccaagct cgattaccat tcagatgacg
     2101 ggaaatgcag aaaagagcga agccctattg cgagtcattc gcccatacgg tattcgcaat
     2161 attgctcgaa cgggtgcaac tggatttacc cgcgattaaa aatccaactt aaatttatta
     2221 aaccagccta aaaggcaata aataatagaa aagagagaaa agctatgaca gttcaaatgg
     2281 aatatgaaaa agatgttaaa gtagcagcac ttgacggtaa aaaaatcgcc gttatcggtt
     2341 atggttcaca agggcatgcg catgctcaaa acttgcgtga ttcaggtcgt gacgttatta
     2401 tcggtgtacg tccaggtaaa tcttttgata aagcaaaaga agatggattt gatacttaca
     2461 cagtagcaga agctactaag ttggctgatg ttattatgat cttggcgcca gacgaaattc
     2521 aacaagaatt gtacgaagca gaaatcgctc caaacttgga agctggaaac gcagttggat
     2581 ttgcccatgg tttcaacatc cactttgaat ttatcaaagt tcctgcggat gtagatgtct
     2641 tcatgtgtgc tcctaaagga ccaggacact tggtacgtcg tacttacgaa gaaggatttg
     2701 gtgttccagc tctttatgca gtataccaag atgcaacagg aaatgctaaa aacattgcta
     2761 tggactggtg taaaggtgtt ggagcggctc gtgtaggtct tcttgaaaca acttacaaag
     2821 aagaaactga agaagatttg tttggtgaac aagctgtact ttgtggtggt ttgactgccc
     2881 ttatcgaagc aggtttcgaa gtcttgacag aagcaggtta cgctccagaa ttggcttact
     2941 ttgaagttct tcacgaaatg aaattgatcg ttgacttgat ctacgaaggt ggattcaaga
     3001 aaatgcgtca atctatttca aacactgctg aatacggtga ctatgtatca ggtccacgtg
     3061 taatcactga acaagttaaa gaaaatatga aggctgtctt ggcagacatc caaaatggta
     3121 aatttgcaaa tgactttgta aatgactata aagctggacg tccaaaattg actgcttacc
     3181 gtgaacaagc agctaacctt gaaattgaaa aagttggtgc agaattgcgt aaagcaatgc
     3241 cattcgttgg taaaaacgac gatgatgcat tcaaaatcta taactaatta gaaatatata
     3301 gcgctggaga tgattttatg aaaaagatta tgagaaaaat tgcatcgtta ttattggttc
     3361 tagttgtata atgtaattat accgtcggta atagtgctag cagaccaaaa taaagcagat
     3421 tggtcgtatg atgaaaatgc tgtaattaac atttatgatg atgctaattt tgaagatggt
     3481 aggttgcata tgacctttga acaattcttc aaattggcac aaatagctag agaagaaggt
     3541 cttgaaattc attctccgtt tgagagagct ggtgcgacta aatctgctcg ttatatagcg
     3601 aaatggattt tgagaaataa aaaatattaa caaatatagt tggtaaatca ttaggaccta
     3661 aatcagctgt tagattcgga gaagctttat cctatattga aggtcctctt cgcagaataa
     3721 atgagacgat agatggcggt ttatatcaaa tagagcaaat tattgcatct ggattgaaag
     3781 aatcgggttt aaatgactgg actgcgaaaa ctttagcttc agctattcgt gggatattag
     3841 atgtacttat ttaggggttg aaatcatatg aatattacca atttgttttc tatcaagaca
     3901 ggatgtgatg aaactgatag gcaactgcaa aaactatttt ttcagttgga tttacaattg
     3961 ggagaattga cagatcaact aagaaaatta gattctaatt ttgttcctcg tagtcaattt
     4021 gtagacacgt tggatttgaa tgatgtagaa tataaagaaa ttttaaacta ttttatcttc
     4081 catcgtaatg atagtgaaga aagtttggta gaatggttat atgattggat ttccacaaat
     4141 cgttatgaac ttcctaaaga gttttcgatt cgtatggctc ataaatacca tgaaagtgtt
     4201 actgaagttt tcggagatga ataactaaaa aacagtcatt agtgactgtt ttttatagaa
     4261 aaagaggttt tatatgttaa gttcaaaaga tataatcaag gctcacaagg tcttgaacgg
     4321 tgtggttgtg aatactccac tggattacga tcattattta tcggagaagt atggtgctaa
     4381 gatttatttg aaaaaagaaa atgcccagcg tgttcgctcc tttaaaattc gtggtgccta
     4441 ttatgccatt tcccagctca gcaaggaaga acgtgaacgt ggggtagtct gcgcttctgc
     4501 gggaaatcat gcgcagggag tagcctatac ttgtaatgaa atgaaaattc ctgctactat
     4561 ctttatgcct attactacgc cacaacaaaa gattggtcag gttcgctttt ttggtgggga
     4621 ttttgtaact attaaactag ttggagatac ctttgatgcc tcagccaaag cagctcaaga
     4681 atttacagtc tctgaaaatc gtacctttat tgatcctttt gatgatgctc atgttcaagc
     4741 aggtcaagga acagttgctt atgagatttt agaagaagct cgaaaagaat cgattgattt
     4801 tgatgctgtc ttggttcctg ttggtggtgg cggtctcatt gccggggttt ctacctatat
     4861 caaggaaaca agtccagaga ttgaggttat cggagtagag gcgaatggag cgcgttccat
     4921 gaaagctgcc tttgaggctg gaggtccagt caaactcaag gaaattgata aatttgcgga
     4981 tgggattgct gtgcaaaagg taggtcagtt gacctatgaa gcaactcgtc aacatattca
     5041 aactttggta ggtgtcgatg agggattgat ttctgaaacc ttgattgacc tttactctaa
     5101 gcaagggata gtcgcagagc ctgctggagc ggctagtatc gcctctttag aggttttagc
     5161 tgaatatatt aaggggaaaa ccatttgttg tatcatttct ggaggaaata atgatatcaa
     5221 ccgtatgcca gaaatggaag agcgtgcctt gatttatgat ggtatcaaac attactttgt
     5281 ggtcaatttt ccacagcgtc caggagcttt gcgtgagttt gtaaatgata tcctggggcc
     5341 aaatgatgat atcacacgtt ttgagtatat caaacgagct agcaagggaa caggcccagt
     5401 attgattggg atcgctttgg cagataagca tgattatgca ggtttgatcc gtagaatgga
     5461 aggttttgat ccagcttata ttaacttaaa tggtaatgaa acgctttata atatgcttgt
     5521 ctgaggacta ataaaaaaat atcatattat ttgtacaact gatgtatatg tgctataatg
     5581 aagatgaaga aaggggacga ttcctatggt tttagaaaac ttatcgtgca attcttactt
     5641 ctgttcatta tagcacttat gatgtttggc tcagatttcc atccttcatt ttgatttcct
     5701 atctattgac aagcatagtc acactgtctt taatactctt cgaaaatctc ttcaaaccac
     5761 gttagctcta tctgcaacct caaaacagtg ttttgagcaa cttgcggcta gcttcc
//
LOCUS       DAVKGC010000112         5534 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKGC010000112 DAVKGC010000000
VERSION     DAVKGC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.112"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            512..964
                     /locus_tag="VW049_001786"
     CDS             512..964
                     /locus_tag="VW049_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9432861.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGDFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            983..2320
                     /locus_tag="VW049_001787"
     CDS             983..2320
                     /locus_tag="VW049_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9432862.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2399..3283
                     /locus_tag="VW049_001788"
     CDS             2399..3283
                     /locus_tag="VW049_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9432863.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3283..4116
                     /locus_tag="VW049_001789"
     CDS             3283..4116
                     /locus_tag="VW049_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9432864.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4311..5414
                     /locus_tag="VW049_001790"
     CDS             4311..5414
                     /locus_tag="VW049_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9432865.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
BASE COUNT         1788 a          888 c         1144 g         1714 t
ORIGIN      
        1 gtttttggtg aaacgagatg tagaaaggag atttagccaa agagtctatt agtgctagaa
       61 taatagatta gaattatttt agaaaaacga agtgagcagc ttataaattc aagtccccaa
      121 atagactcat actagtatct tttgcaaaaa ataaagggcg acttccttca tgaatatcaa
      181 tttcatctat aaggaaggta gctaattgaa ctaacttatt tattctgttt gtcgctagaa
      241 aaatcagacc tccttgtgaa gattgaggag atacttaatg aaaatcaaag aagaaactag
      301 caagctagta gcagattgcc caaaacaccg ctttgaggtt gtagataaga ctgacctata
      361 taatccaagg tgaagcgact gtggtttgaa gagattttca aagagtatag actagagagt
      421 agtgttttta tgtccttcta gtagaaaatg ctagacagaa gaatggggaa cttggatagg
      481 aaaaatagat tgagaaagga ggttagaaga gatgattatt acaaaaatta gccgtttagg
      541 aacttatgtg ggagtcaatc cacattttgc aacattaata gattttctag aaaaaacagg
      601 actagaaaat ttaacagaag gttcgattgc tatcgatggt aatcgattgt ttgggaattg
      661 ctttacttat ctagcagatg gtcaagcagg ggatttcttt gaaacacacc aaaaatattt
      721 ggatattcat ttagttttgg aaaacgaaga agccatggct gttacatcgc cggaaaatgt
      781 aagcgttacc caagaatatg atgaagagaa agatattgaa ttatacacag ggaaagtgga
      841 acagttggtt catttgagag ctggtgaatg cctcatcact tttccagaag atttacatca
      901 acccaaggtt cgtataaatg atgaacctgt gaaaaaagtt gtctttaaag ttgcgatttc
      961 ttaatgtaga aagagaagaa cgatgaaaaa aatgagaaag tttttatgtc tagctggaat
     1021 tgcgctagcg gctgttgcct tggtagcttg ttcaggaaaa aaagaagcta caactagtac
     1081 tgaaccacca acagaattat ctggtgagat tacaatgtgg cactccttta ctcaaggacc
     1141 ccgtttagaa agtattcaaa aatcagcaga tgctttcatg caaaagcatc caaaaacgaa
     1201 aatcaagatt gaaacatttt cttggaatga cttctatact aaatggacta caggtttagc
     1261 aaatggaaat gtgccagata tcagtacagc tcttcctaac caagtaatgg aaatggtcaa
     1321 ctcagacgct ttggttccgc taaatgattc tatcaagcgt attggacaag ataaatttaa
     1381 cgaaactgcc ttaaatgaag caaaaatcgg agatgattac tactctgttc ctctttattc
     1441 acatgcacaa gtcatgtggg ttagaacaga tttgttaaaa gaacataata ttgaggttcc
     1501 taaaacttgg gatcaactct atgaagcttc taaaaaattg aaagaagctg gagtttatgg
     1561 cttgtctgtt ccgtttggaa caaatgactt aatggcaaca cgtttcttga acttctacgt
     1621 acgtagtggt ggaggaagcc tcttaacaaa agatcttaaa gcagacttga caagccaact
     1681 tgctcaagat ggtattaaat actgggttaa attgtataaa gaaatctcac ctcaagattc
     1741 tttgaacttt aatgtccttc aacaagctac cttgttctat caaggaaaaa cagcatttga
     1801 ctttaactct ggcttccata tcggaggaat taatgccaac agtcctcaat tgattgattc
     1861 gattgatgct tatcctattc caaaaatcaa agagtctgat aaagatcaag gaattgaaac
     1921 ctcaaacatt ccaatggttg tttggaaaaa ttcaaaacat ccagaagttg ctaaagcatt
     1981 cttagaagca ctttataatg aagaagacta cgttaaattc cttgattcaa ctccagtagg
     2041 tatgttgcca actattaagg ggattagcga ttctgcagcc tataaagaaa atgaaactcg
     2101 taagaaattt aaacatgctg aagaagtaat tactgaagct gttaaaaaag gtactgctat
     2161 tggttatgaa aatggaccaa gtgtacaagc tggtatgtta actaaccaac acattattga
     2221 acaaatgttc caagatatca ttacaaatgg aacagatcct atgaaagcag caaaagaagc
     2281 agaaaaacaa ttaaatgatt tatttgaggc tgttcagtag atgtaaaaga ctagaaaata
     2341 ggtgggctag tgagctgaaa agctctagcc caatcttgta aaagaaggga gaaggagaat
     2401 ggttaaagaa cgtaatttaa ctcgctggat atttgttttg ccagctatga ttatcgtagg
     2461 attactcttt gtttatccgt ttttctcgag tattttttat agctttacca ataagcattt
     2521 gattatgcct aattataaat ttgttggttt ggctaactat aaagctgtgc tatcagatcc
     2581 caacttcttt aatgcgttct ttaattcaat taagtggacc gttttctcat tagttggtca
     2641 agttttagta gggtttgtat tggctttagc tcttcacaga gtacgccact tcaagaaatt
     2701 atataggacc ttattgattg ttccttgggc atttcctacc atcgttattg ccttttcttg
     2761 gcagtggatt ctaaacgggg tttatggcta cttacctaat ctaatcgtaa aattaggttt
     2821 aatggaacat acacctgcat ttttgacaga tagtacatgg gcattcctat gtttggtgtt
     2881 tatcaacatt tggtttggag caccaatgat tatggttaat gtgctttcag ctttgcaaac
     2941 agtaccagaa gaacaatttg aggctgctaa gatagatggt gcttcaagtt ggcaggtgtt
     3001 caagtttatc gtctttccac atattaaagt ggttgtagga cttctagttg ttttgagaac
     3061 tgtatggatc tttaataact ttgacattat ctacctcatt actggtggtg gaccagccaa
     3121 tgctacaacg acgcttccaa tttttgctta caacctgggc tggggaacta aattgttggg
     3181 tcgtgcttca gcagttacag tattgctctt tatcttcttg gtggcgattt gctttatcta
     3241 ctttgctatc atcagtaagt gggaaaagga gggtagaaaa taatgaagaa gaaatccagt
     3301 atttatttag atattctctc acatgtactt ttagttggtg cgaccatcgt tgcagttttc
     3361 ccattggtat ggattatcat atcttctgtc aaagggaaag gggaattaac tcagtatcca
     3421 acacgatttt ggcctgaaca gtttacatta gattatttca ctcatgttat caacgatttg
     3481 cacttcattg ataacattcg aaacagttta atcattgcct tggctacaac ccttattgcg
     3541 attattattt ctgctatggc agcctatggt attgttcgat tctttcctaa attgggagca
     3601 atcatgtcga gactactcgt cattacctac attttcccac caattttgtt agcaattccc
     3661 tattcaattg ccattgctaa agttgggtta acaaatagtt tatttggctt gatgatggtt
     3721 tatctatctt ttagtgttcc atatgcagtt tggctcttag ttggattttt ccaaacagtt
     3781 ccaattggaa ttgaagaagc ggctagaatt gatggtgcaa ataaatttgt tacgttttat
     3841 aaagttgtgc taccgattgt agcaccaggt attgtagcaa cagctattta tacatttatc
     3901 aatgcttgga atgaattcct gtatgccttg attttgatta acaatacagg aaagatgaca
     3961 gtagcagtag cccttcgttc acttaatggt tcagaaatac tagactgggg agatatgatg
     4021 gcagcgtctg ttattgtagt tcttccatca attattttct tctctatcat ccaaaataag
     4081 attgcaagtg gattatcaga aggatctgtg aagtaatact caatgaaaat caaagagcaa
     4141 actaggaaac tagccgcagg ctgctcaaag tacagctttg aggttgcaga taaaactgac
     4201 gaagtcagta accatacgca cagtaaggcg acgttgacgc ggtttgaaga gattttcgaa
     4261 gagtataagt taaaattaga aagtttattc aataataaag gagaaaaaac atggttaaat
     4321 acggtgttgt tggagcaggg tattttggag ctgaattggc tcgctacatg caaaaaaatg
     4381 atggagcaga gattactctt ctctatgatc cagataatgc agaggcgatt gcagaagaat
     4441 tgggagcaaa agtagcaagt tccttagatg agttggtttc tagcgatgaa gtagattgtg
     4501 ttatcgtcgc aactccaaat aatcttcata aggaaccggt tattaaggct gcacagcatg
     4561 gtaaaaatgt tttctgtgaa aaaccaattg cgctttctta tcaagattgt cgcgagatgg
     4621 tagatgcgtg taaagaaaac aatgtaacct ttatggcagg acatattatg aatttcttta
     4681 atggtgttca tcatgcaaaa gaactcatca atcaaggagt tatcggagac gttctatatt
     4741 gtcatacagc tcgtaatggt tgggaagaac aacaaccgtc agtgtcatgg aaaaaaattc
     4801 gtgaaaaatc aggtggtcac ttgtatcacc acatccatga attggattgc gttcaattcc
     4861 ttatgggggg catgcctgaa actgtaacca tgacaggtgg aaatgtggcc catgaaggtg
     4921 aacatttcgg tgatgaagat gatatgattt ttgtcaatat ggaattttct aataagcgtt
     4981 ttgccttgtt agaatggggt tcagcttatc gttggggtga acattatgtc ttaatccaag
     5041 gaagcaaagg tgccatccgc ttagacttat tcaactgtaa aggaactctt aagctagatg
     5101 ggcaagaaag ctatttcttg attcacgaat cgcaagaaga agatgatgat cggactcgta
     5161 tctatcatag tacagagatg gatggagcaa ttgcttatgg taaaccaggt aaacgtactc
     5221 cattatggct atcatctgtc attgataaag aaatgcgcta tctgcatgag attatggaag
     5281 gagccccagt atcagaagaa tttgcaaaac ttttgacagg tgaagctgcc ctagaagcaa
     5341 ttgctactgc agatgcttgt acccagtcta tgtttgaaga tcgcaaagta aaattgtcag
     5401 aaattgtaaa ataaattttg gtattctcct atttataggt cgacttgctc ctctgaaagt
     5461 acttttagag gagctgtttg actttgctag tttttgaaac tgaaatctat tatactacaa
     5521 actattgaaa gcgt
//
LOCUS       DAVKGC010000113         5331 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKGC010000113 DAVKGC010000000
VERSION     DAVKGC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.113"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            205..657
                     /locus_tag="VW049_001791"
     CDS             205..657
                     /locus_tag="VW049_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432866.1"
                     /translation="MIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIV
                     AIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTIMMNTSNDLFI
                     KAIELLPFYSLPLFVVSNTMSISQYLVLITTIILLYFFTLKKSKKYSF"
     gene            1043..1252
                     /locus_tag="VW049_001792"
     CDS             1043..1252
                     /locus_tag="VW049_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9432867.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            1242..1730
                     /locus_tag="VW049_001793"
     CDS             1242..1730
                     /locus_tag="VW049_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432868.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            1846..3306
                     /locus_tag="VW049_001794"
     CDS             1846..3306
                     /locus_tag="VW049_001794"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9432869.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLCNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            3303..4127
                     /gene="nadE"
                     /locus_tag="VW049_001795"
     CDS             3303..4127
                     /gene="nadE"
                     /locus_tag="VW049_001795"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9432870.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKNFV
                     LGISGGQDSTLAGRLAQLAIEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            4425..4976
                     /locus_tag="VW049_001796"
     CDS             4425..4976
                     /locus_tag="VW049_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9432871.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKVYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
BASE COUNT         1585 a          952 c         1157 g         1637 t
ORIGIN      
        1 agtttgctta tctcgtttat accgccgata ttgcttacat tctttattct aaagactcaa
       61 gagacatcgc ttggtattaa gcatatttca aacttttatg ctatgctcgg tatgttaatg
      121 gctgttatac atgctaatcg agttattagt agagattttt cccacaatac ggtaagtttg
      181 ttttataatc aacagaagaa tcggatgatt tacgtcttgt ctaattttct atatgccatc
      241 tcagtttcca ttatttatgc tttgaatggc attgtgttac tagtcatagt aagtaagttg
      301 ggtattccag gtgatttagg attagatttt atagtagcta ttgtagtcaa tacaattttg
      361 ttagtcctgt tttattttct attatcttac attttctatt tatacaaatt gaaaagtggc
      421 ttggtatttg gtattttagt agctttacta ctctttatcc ctaatatatt aaatacgatt
      481 atgatgaata ctagtaatga tttgtttatc aaagcaattg aacttcttcc tttttattct
      541 ttacctctat ttgtggtttc aaatacgatg tctattagtc agtatcttgt gctaatcact
      601 acaatcattt tattgtactt tttcactctc aagaaaagca agaagtattc attttagttt
      661 catttagtat tattttgcat acctaaaata cagtaaaaaa atcagtcatc ttggtatgct
      721 cctgctttca ctattcaaca cgtttttgac ttatactagg ctcatttcca aaagcattat
      781 ataatagtga tatgaaacca actaaactaa acaagaaata taagcaataa aaactcgttt
      841 aaaagattct actaaagcta atactaaata aaaataaaag agtaaactag gaaatttatt
      901 tcaaacaacc taaaatactg attttcgtct gaagataata ctggagtgca aattaatggg
      961 gttataataa atagctgata gcttgtgttg gttttggatt ttttaagagt agatgagtat
     1021 taaaactata aggaggacga aggtggctaa aaatttaaaa ttaaaattag ctcgggtaga
     1081 gcgtgattta acacaaggtc aactggcaga ggctgtcggg gtgacacgcc agactattgg
     1141 tttgatagag gcgggaaaat acaatcccag tctctcgctc tgccagtcta tttgcagatg
     1201 tttagggaaa accctagacc aactattttg ggaggaagaa gatgaaaaat agattttatt
     1261 attatcaatt actagacgaa agagaagaac aactgttcaa taaagcggga tctgaaagtt
     1321 tctatatctg cattgctttg tcgctcctat cttatatcat ttcagtatta gcaccaagcc
     1381 tttttaattc taatatgctg ctaatcgtta tcatcatagg gacattttac tttttcaatc
     1441 gcgcccgtta tctgggagtg acctactata gtcgttttca ttttacgatt ttgggttgtt
     1501 ttttcctaac cttggctatt acggctcttt tgatgttgca gaattatcaa ttcaacatag
     1561 aaatttatca gcacaatcct ttgaatttta aatacctgtc tgcttgggtc attacttatg
     1621 tcatttacct tccgtgggtc tttattggca atcttggtct taagagctat ggcgaatggg
     1681 ctcagaaaaa atttgaacaa gatatggatg aattggagag tggagaatag cttgttactc
     1741 ttttctcaat ccagctaaaa tgtgatataa tagtactaat ttattggaat acatgaaagt
     1801 tcttgaaaat tttcatgggt ttctagctaa ggaagtagga aaagtatgta tccagatgat
     1861 agtttgacat tgcacacgga cttgtaccag atcaacatga tgcaggttta ctttgaccaa
     1921 gggattcaca ataagaaggc ggtctttgag gtgtatttcc gccaacagcc ttttaagaac
     1981 ggctatgcgg tttttgcagg tttggaaaga attgtgaact atcttgaaga cttgcgtttt
     2041 tcagatagtg atatagccta tttggagtcg cttggttatc atggggcgtt cttggattac
     2101 ctttgcaatt tcaagttgga gttgaccgtt cgttctgccc aagaagggga tttggttttt
     2161 gctaatgaac cgattgtgca ggtggaagga cctctagccc aatgtcagtt ggtcgaaacg
     2221 gctcttttga acatcgtcaa ctaccagacc ttggtggcga cgaaggcagc tcgtattcgt
     2281 tcggttatcg aagatgaacc cttgatggag tttgggacac gtcgggctca agaaatggat
     2341 gcggccatct ggggaacacg tgcagcggtg attggtggcg ccaatggaac cagcaacgtg
     2401 cgtgcgggta aactctttga cattcctgtt ttgggaaccc atgcccatgc cttggtgcag
     2461 gtttatggca atgactatga ggctttcaag gcttacgctg cgacccacaa aaattgtgtc
     2521 tttcttgtgg atacctatga tacccttcgc atcggtgtac cagctgccat tcaggtggcg
     2581 cgtgaactgg gtgatcagat taactttatg ggtgtgcgga ttgactctgg ggatattgct
     2641 tacatttcta agaaagtccg tcagcaactg gacgaggctg gatttacaga ggctaagatt
     2701 tatgcttcta atgatctaga tgaaaatacc atccttaacc tcaagatgca aaaggccaag
     2761 attgatgtct ggggtgtggg taccaagctg attacagcct atgaccagcc ggctcttggg
     2821 gcggtttaca agattgttgc aattgaagat gaaactggtc agatgcgcaa tacgattaag
     2881 ctgtctaata atgcggaaaa agtgtcgacg ccaggtaaga agcaggtgtg gcgcattacc
     2941 agtcgtgaaa aaggcaagtc agaaggtgac tacatcactt atgatggtgt ggatattagc
     3001 gacatgacag aaatcaagat gttccatccg acctatacat acatcaagaa gacggttcgt
     3061 aattttgatg ccgttcctct cttggtggat atcttcaaag aaggaatatt agtttacaac
     3121 ttgcctagtt tgactgacat tcaggattat gcccgtaaag aatttgacaa gttgtgggat
     3181 gagtataagc gtgtgctcaa tccgcagcac tatccagtgg atttggcacg tgatgtatgg
     3241 caagataaga tggacttgat tgataagatg cgcaaggaag cccttggtga aggagaagaa
     3301 gaatgagttt gcaagaaacg attatccaag agctgggtgt caaaccagtg attgatgccc
     3361 aggaagaaat ccgtcgttct attgatttct taaaaagata tctgaaaaaa catcccttcc
     3421 taaaaaactt tgtactaggg atttctgggg gacaagactc aaccttggca ggacgtttgg
     3481 cgcaattagc tatagaagaa ctgcgagctg aaacgggaga cgacagctac aaatttatcg
     3541 ctgtccgcct gccatacgga gtgcaagctg atgaagcaga tgctcaaaaa gccctagcct
     3601 tcatccagcc agatgtcagc ttggttgtga atatcaagga gtcagctgat gctatgacag
     3661 ctgcagttga agcgacaggt agtcctgttt cagacttcaa caaggggaat atcaaggcac
     3721 gttgccgtat gattgctcag tatgcccttg ctggttccca tagcggagcg gtcattggaa
     3781 cagaccacgc cgcggaaaat atcacaggtt tctttaccaa gtttggtgac ggcggtgcgg
     3841 atattctccc tctttaccgc ctcaataaac gccaaggaaa acagctcttg cagaaacttg
     3901 gcgcagagcc agccctttat gaaaaaatcc caacagcaga cctagaagaa gataaaccag
     3961 gcctagctga cgaagtcgca cttggagtca cctacgcaga gattgacgac tacctagaag
     4021 gcaaaacaat cagcccagaa gctcaagcga ccattgaaaa ctggtggcac aaaggccaac
     4081 acaaacgcca cttacccatc accgtatttg atgacttttg ggagtaaaaa cctccattgg
     4141 aggttttcag cctctcatct tgaaataaga aagtgagaga aggtctgggg gatcttgaac
     4201 cccgagttta gaaataagaa aatgaggcag attcggtaac tcgaagagtt cgatttcatc
     4261 gtcttacccc tgcaacgatg actaggtttg aaaaagcttg ctagagcgca tttcaaacca
     4321 ggcagcgact gcgtcaagaa attagaagac aaactcgttt tctagctgtt actgagttga
     4381 gcctttttac tacgagcata gaaataagga agtgaggtag catcatgaaa tctatcggta
     4441 cgcaaatatt acagacagaa cgtttgattt taagaagatt tgtggagagt gatgcagaag
     4501 ctatgtttca aaattgggct tcatccgctg agaatttgac ctatgttacc tgggatcccc
     4561 atcctgatgt cgaaatcact cgaaactcga ttcgcaattg ggttgcttcc tatactaatc
     4621 tcaactatta caaatgggcc atttgtctaa aagaaaaccc agagcaagtg ataggagata
     4681 tcagcatcgt taagatagac gaggctgatt taagctgtga aatcggctat gtgctaggca
     4741 aggtttactg gggacatggt atgatgacag aggccttgaa agctgtcttg gacttttgtt
     4801 ttactcaagc aggttttcaa aaggtcagag cacgttatgc cagtctcaac ccagcttcag
     4861 gtcgtgtcat ggaaaaggct ggaatgtcct atctacaaac cattgttaat ggtgtagaga
     4921 gaaaaggcta tcttgcggat cttatttatt atggtataag tagggaagaa tgttgaattc
     4981 tattttctgt ttctatcgaa gtcaactatt tattgtaaat ataataatta gcattccaag
     5041 tttatttgca attttaaaat agcatattga ttattacaag acagatgctc tagttccttc
     5101 tttaatctgg tttagtatta gttaaaaaat cgcttgaagc ttgtaactaa gagggagcta
     5161 atcgactagg ttctccagcc gaacaggtgg taatgtactt tttatagtgt aatcctagct
     5221 gttgttaaat ttaaaataga atcctctatc gagttaggga attaaattca accaatttta
     5281 tttatgtttt tctatcaaat tatctaatat taaaatagtc tcattctgat g
//
LOCUS       DAVKGC010000114         5327 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKGC010000114 DAVKGC010000000
VERSION     DAVKGC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.114"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..285
                     /locus_tag="VW049_001797"
     CDS             <1..285
                     /locus_tag="VW049_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9432872.1"
                     /translation="NKDPEKFFGLIEDNLKQVHPLFQTVFKTFLKNKEKIVNALQLHY
                     SNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERAKFVLSQA"
     gene            complement(359..2812)
                     /gene="phtA"
                     /locus_tag="VW049_001798"
     CDS             complement(359..2812)
                     /gene="phtA"
                     /locus_tag="VW049_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU9432873.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLAPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVTLARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNEDVDSLLKQLYALPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYH
                     FIPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQQTPEPSPGPQPAPNLKIDS
                     NSSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKK
                     ENVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDD
                     LLAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAY
                     VTPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRV
                     KGEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDL
                     FATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEV
                     PQVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEK
                     LLALLKGSNPSSVSKEKIN"
     gene            complement(3066..4799)
                     /gene="ptsP"
                     /locus_tag="VW049_001799"
     CDS             complement(3066..4799)
                     /gene="ptsP"
                     /locus_tag="VW049_001799"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9432874.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKEAGEAYAKQKAEWALLKDAKTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVAYNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(4805..5068)
                     /locus_tag="VW049_001800"
     CDS             complement(4805..5068)
                     /locus_tag="VW049_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9432875.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT         1439 a         1127 c          986 g         1775 t
ORIGIN      
        1 aacaaagacc ctgagaaatt tttcggactc attgaggaca atctgaagca ggttcatcct
       61 ctttttcaga ctgtctttaa aacctttcta aagaacaaag agaaaatcgt caacgccctt
      121 caactacact attctaatgc caaactggaa gcgaccaata atctcatcaa acttatcaag
      181 cgcaatgcct ttggttttcg gaactttgaa aacttcaaaa aacggatttt tatcgccctg
      241 aatatcaaaa aagaaagggc gaaatttgtc ctttctcaag cttagctttt tttcaaccca
      301 ctacagttga caaagagcct aaaaatgaaa gcctctttat cgagactttc atttttcatt
      361 agtttatttt ttccttactt acagatgaag gattacttcc ttttaacaac gcaagtaatt
      421 tttctgcttc tgccatgata ctattgttat ccataatttg aagagtcaaa ttatttcgta
      481 aaccagctag agtttctgtt gcattggctt tcagactaga atccgttact ttcgcaagca
      541 aaacttctgc ttctttgagt tgggcttcta ctttttcagt ctctacttga gggacttctg
      601 gctcagcagg tgtttcctct actggctctt catccgcttt gaagttctta tttggatctt
      661 cactgtggtc tttcttgcct aacacatgct cactggcatt gccccatcca tcattagaat
      721 gtggacgttc gtcagggtgt tctacgtagt acttaatcgt cgcaaacaaa tcttccaagg
      781 tatagccatt tggagctttg tatgtgtgat catcaaacca agcaaattta atgttatggt
      841 aatgatcctt atgaggaata atcaaattac cgtttttaac ctcaactgta tgctcaacca
      901 tatatggaag tcgaacgagt ggaattcgtt tttccccttt cacacgattg taaatagctg
      961 ctgcactatc tccagttgga tttgctttaa catctgcgtc tggagatgga ggtaggatac
     1021 ctttttcttt agtataggct tgagctgcaa ctttttcctt atcagaaagg ctgtcttttc
     1081 caatccagtg actatggccc atatgaggcg ttacatatgc atctccttca tcactgatta
     1141 tatcatgttc atcaaaaatg taaccatctg acgttgtata cttatcagct aattgagcaa
     1201 tacgaacttc gtcttcagta tactcaattt gagaatttgg cttgccaagt cgctctggat
     1261 gggtaattgg tgctaggaat gccaataaat catctaccaa tttttcttta ttagtcgatt
     1321 catcattcaa gcgttctaat aatttgtcta aggcttggaa atcagaatta cgacccttat
     1381 tttcaaacaa ggctttatga gcctcagtta acagattata tgctttatca taaaattctt
     1441 ggtcacgagg agcaacattt tcttttttag cagttaaagt gtgtgaaaca ctctcttgtt
     1501 ttgataactt gctttcaaga tttttaacag tttcagatgg taaatctttc gcaaaaacat
     1561 aacgagagat gcccttttct tcgaatacat atccttcccc aacttttcgt accagctgac
     1621 taaccaaaga agaatttgag tctattttaa gatttggtgc aggttgcggg cctggactag
     1681 gttccggagt ctgttgtgga cttggttgtt ctggccttga atccggtacc caatggtttg
     1741 aacgataacg aaggggaata atacgagcga ttcgttcttc caattcagac atttgagagt
     1801 aagggatgaa gtggtaatga tctccgtgtg gcactgcaac acctctagct gttcgacttg
     1861 tgatttgtgc tggatcaaag acaaggccat cagattctac gtgtcgttgg ctgagtggca
     1921 aggcataaag ttgtttcaga aggctatcaa catcctcatt actttgactt gcttgactgt
     1981 tagtgttgct gttgttactt gtgttagtat ttgtagttcc tggattgctt acagaaggta
     2041 cccagtttgt tcttgaagtg ttatcgctat tttgtcggcg ataggttctt gaatttgaca
     2101 gatttccccg accagatagg aaggcttctg cagcagctaa ctcgctagct gataactcat
     2161 tcttaggaat gtaatggtaa tgatctccat gaggaacgat ataagcatca ccagtatcct
     2221 ctatgatatc agaagcatta aagatataac catcatctgt agtatagcgt ccttgcgaac
     2281 gtgccaaggt aacagcacca tcgtttcttg gagtcccacc ttcacgatgt tgactatgtt
     2341 cttgtttttg tcgattgatt tcctcttttg tacggacatt atccgcgtgg gcagcatcct
     2401 taaggtaaac atagtatttt ccatctacct tgataacata tccacccttg acctcattaa
     2461 caatatcctc atcttttagc ttatagtttg gatctttcat gagtaattct tcactgaaaa
     2521 tcgcatcata aggaacctta ccattgtaat aatgatagtg gtcgccatgt gaagtgacat
     2581 agccttggtc tgttatcttg atgacgattt gctcagcatt gattccttca cgcttgctaa
     2641 cctcatcagg agccaaattc tccgtttttt gcgtcgcttg ttttccatct atataggaaa
     2701 cacgattatt ttccttaacc gttctagctt gatacagtcc caactcgtaa gaacaaacac
     2761 ttaaaatcaa agctgccgca gaaccaacaa ggtatttctt attaattttc atcgaaactc
     2821 tctttctatt tgatatttct agttgcaatt catttctaaa ttgcagaaac tcccctctat
     2881 tacttaactg gttaatagtt tactcctaaa tctccaactt gtcaagaatt ttatgaacca
     2941 tttaagtatt ttttgttctg accactactt tttcaaggtt ggactttcta taagaaagat
     3001 tttcttgaaa attcttttta aaatatcaaa aaaatccctg taactgagtt gcagggactt
     3061 ttcgattaat caaaattaac gtattctttt tgaagttcaa gaacttcttc cattgttgag
     3121 cattctgtaa gggcacggtt tgcgtactct tccatcttag ctgtgtcgag tttcttcatc
     3181 aagctgcgtg tacgaagtac agatgttgct gacatagaga actcatccaa gcccattccg
     3241 acaagaagtg gaacagcttg ttggtcacca gccatctcac cacacatacc agcccattta
     3301 ccttcagcgt gagctgcttt gatcacattg ttaatcaagc gtaggattga tgggttgtat
     3361 ggttggtaaa ggtatgaaac ttgttcgttc atacggtctg ctgccattgt atattggatc
     3421 aagtcatttg taccaattga gaagaagtca acttctttag caaattggtc tgcaagcata
     3481 gccgctgcag gaatctcgat catgatacca acttgaatgt tatacgcaac tgcaacacct
     3541 tcagcaagaa ggtttgcttt ttcttcatca aagactgctt tcgctgcacg gaattctttc
     3601 aagagcgcaa ccattgggaa catgatacgc aattgaccgt gaacagacgc acgaagaaga
     3661 gcacggattt gtgtgcggaa catagcatct ccagtctcag agatagagat acgaagagca
     3721 cggaatccaa ggaatgggtt catttcgtga ggcatatcga agtaaggaag ttccttatct
     3781 ccaccgatat ccattgtacg aacgacaaca ggtttaccgt tcattccttc aagaacagcc
     3841 ttgtatgctt catactgctc atcttcagtt gggaagtctt gagaatccat gtacaagaac
     3901 tctgtacggt aaagtccaac agcttctgca ccgttgttgt taacaccttc aacgtctttt
     3961 ggagtaccga tattagcagc caactcgaag tgtttaccgt cagcagtcac tgttttagcg
     4021 tctttcaaga gcgcccattc agctttttgt tttgcatagg cttcaccagc ttctttaaat
     4081 tctgccgctt gttcatcagt tgggttgata ataacctcac cagtgatccc gttaacagca
     4141 aggatgtcac cgtctttaac gatttcagtg atgttatttg tacctaatac agcagcaatt
     4201 tcaagtgtac gtgccatgat agctgagtgg cttgtacgtc caccgatgtt ggttacaaaa
     4261 gcttttacaa agtttttgtc caattgagct gtatctgaag gagtcaagtc atgcgcaatc
     4321 acaatcactt cttcattgat agaagctggg tttggcaatt ttttaccaag aaggtttgcc
     4381 aatacacgtt ttgtcacgtc gcggatatcc gctgcgcgtt cttgcatgta tgggttgtct
     4441 tccatgcctt caaagatagt gataaacata tctgtaactt ctttcagacc tgcttctgca
     4501 ttcactttct tcgcacggat agtttccttg atttggctga tcatttctgg gtcagcaaga
     4561 accattaagt gagcatcaaa aacttgagct gcttcttcac cgagcgtacc tactgctttc
     4621 tcgcgaataa cagaaagctc gtcttgtgat gcctgtagag cggcatcaag gcgagcttct
     4681 tctgcgtttg tatcttcgac tgtaatagtc tcaaatgaca aatccggctg aacgagtaga
     4741 tatgcttttg caactgcaac accgtcagat gctgcgattc ctttaagcat ttctgtcatt
     4801 tcccttatgc caatccttct ttttccattg tttctgagat tgcagcgata gcgtcatctg
     4861 catctgcacc ttcagctgag atagttacgt cagcaccttg accaacacca agactcataa
     4921 cacccataat tgatttaagg ttaactgatt tacctttgta ctcaagagtg atatctgaag
     4981 caaatttgct agcagtttgt accaacaatg ttgctggacg tgcgtgaata cctgtttctg
     5041 ccactacgtg gaaatcttta gaagccatag tttgactctc cttttgttct ttcttttttt
     5101 gagttatatg tgataaccct tacaatttag tattatacca tcttctagga tttttttcaa
     5161 gcattttctg gattttcctt caatttcctt tcagataact tgctgaaaat cttctatcct
     5221 agataacaaa acacaggata tagtttcttc aaaaacaatt tataggataa tcattgctat
     5281 ttcacaatac aaacactata tattgtgttt atatatgaaa acaacaa
//
LOCUS       DAVKGC010000115         5292 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKGC010000115 DAVKGC010000000
VERSION     DAVKGC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.115"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            26..831
                     /locus_tag="VW049_001801"
                     /pseudo
     CDS             26..831
                     /locus_tag="VW049_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            853..1592
                     /locus_tag="VW049_001802"
                     /pseudo
     CDS             853..1592
                     /locus_tag="VW049_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            1605..2771
                     /locus_tag="VW049_001803"
     CDS             1605..2771
                     /locus_tag="VW049_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9432876.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYV
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFLASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            3279..4772
                     /locus_tag="VW049_001804"
     CDS             3279..4772
                     /locus_tag="VW049_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9432877.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            4785..>5292
                     /locus_tag="VW049_001805"
     CDS             4785..>5292
                     /locus_tag="VW049_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9432878.1"
                     /translation="MKKVKLGEILSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLL"
BASE COUNT         1684 a          934 c         1124 g         1550 t
ORIGIN      
        1 tttgaatata gagagttttc tgacaatgaa tcaatcctac ttttatctaa aaatgaaaga
       61 acacaaactc aaggttcctt atacaggtaa ggagcgccgt gtacgtattc ttcttcctaa
      121 agattatgag aaagatacag accgttctta tcctgttgta tactttcatg acgggcaaaa
      181 tgtttttaat agcaaagagt ctttcattgg acattcatgg aagattatcc cagctatcaa
      241 acgaaatccg gatatcagtc gcatgattgt cgttgctatt gacaatgatg gtatggggcg
      301 gatgaatgag tatgcggctt ggaagttcca agaatctcct atcccagggc agcagtttgg
      361 tggtaagggt gtggagtatg ctgagtttgt catggaggtg gtcaagcctt ttatcgatga
      421 gacctatcgt acaaaagcag actgccagca tacggctatg attggttcct cactaggagg
      481 caatattacc cagtttatcg gtttggaata ccaagaccaa attggttgct tgggcgtttt
      541 ttcatctgca aactggctcc accaagaagc ttttaaccgc tatatcgagt gccagaaact
      601 atcgcctgac cagcgcatct tcatctatgt aggaacagaa gaagcagatg atacagacaa
      661 gaccttgatg gatggcaata tcaaacaagc ctatatcgac tcgtcgcttt gctattacca
      721 tgatttgata gcagggggag tacatctgga taatcttgtg ccatccatag tgaaatccct
      781 tggtcagaaa atctaccaga ttgtctgaga ttttttgcag aaaaatggta agttaagaaa
      841 ggaaaaaacg aaatgcatat tgaacatctt agccactgga gtggtcatcc taaccgtgaa
      901 atgtacctta accgttatgg acatggtggg attccagttg tggtctttgc ttcatcaggt
      961 ggtagtcaca acgaatacta tgattttggc atgattgatg cctgtgcttc ctttatcgag
     1021 gaaggccgtg tccagttctt taccctatct agtgtggata gtgagagctg gttggctact
     1081 tggaaaaatg ctcatgacca agcggaaatg caccgtgcct acgaacgtta tgtgattgag
     1141 gaggccattc ttttatcaag cacaagacag gttggtttga tggcatgatg acgacaggtt
     1201 gctctatggg agcctatcat gcacttaatt tcttcctcca gcatccagat gtctttacca
     1261 aagtgattgc tctcagtggt gtttacgacg cacgtttctt tgtcggtgat tactacaacg
     1321 atgatgctat ttaccaaaac tcgccagtag attatatttg gaaccaaaac gacggctggt
     1381 ttattgaccg ttaccgtcag gcagagattg tgctgtgtac ggggcttgga gcctgggaac
     1441 aagatggttt gccatccttt tacaagctca aagaagcctt tgacaagaaa caaattccag
     1501 cctggtttgc tgaatgggga catgatgtcg cccatgactg ggaatggtgg cgtaaacaaa
     1561 tgccttattt cctcggtaat ctctatttat aaaaggagtt acctatgaat taccttgtta
     1621 tttctcccta ctatccacaa aactttcaac agtttaccat cgaactagct aataaaggca
     1681 tcacagtctt gggaattggt caagagtctt acgagcaatt ggatgagccc ttgcgcaata
     1741 gcttgaccga gtattttcgt gttgataatc ttgagaacat agatgaagtc aaacgtgcag
     1801 ttgcttttct cttttataaa catggtccaa ttggccgcat cgagtctcac aatgaatact
     1861 ggcttgagct agacgcaaca ctcagagaac aattcaatgt ttttggtgcc aaaccagagg
     1921 atctcaaaaa gacgaaatat aagtctgaaa tgaagaaact tttcaaaaaa gcaggtgttc
     1981 ctgtggtacc tggagctgtt atcaagacgg aagcagatgt tgatcaagca gtgaaagaaa
     2041 tcggtcttcc aatgattgcc aaacctgata atggagtggg agcagccgca acctttaaac
     2101 ttgagacaga agacgatatc aatcacttca agcaagaatg ggaccattca accctttatt
     2161 tctttgaaaa atttgtcact tccagcgaaa tctgtacctt tgacgggctc gtggacaagg
     2221 atggaaagat tgtcttctca acaacctttg actacgtcta tacaccgctt gacctcatga
     2281 tttataagat ggacaattct tattatgtgc tcaaggatat ggatcctaaa ctgcgcaagt
     2341 atggggaagc aattgtcaaa gaatttggta tgaaagaacg gtttttccat attgagttct
     2401 tccgtgaggg ggacgattat attaccatcg agtacaataa ccgccctgca ggtggtttta
     2461 ccattgatgt ttataacttt gctcattcct tggaccttta tcgtggctat gcagctattg
     2521 tcgcaggaga ggagttcctg gcgtcagact ttgaaactca gtattgtttg gctacttctc
     2581 gccgtgcaaa tgctcactat gtttattcag aagaggattt gcttgccaaa tatagccagc
     2641 agttcaaggt taaaaaagtc atgccagctg ccttcgcgga acttcaagga gattacctgt
     2701 atatgctgac cactccgagt cgacaagaaa tggagcagat gattgcagat ttcggacaac
     2761 gtcaagaata agaactatcg gattaaggaa attcactccc ttaatccttt tgttttgtct
     2821 ggtaaaaaat aagagcatcc taacaaggta gctatcataa aacttgttcg ataactattt
     2881 gaagcaggat taggtggcta gaaattgaat tttaacattt caattgagtc atagtattgt
     2941 gttttcgtat ccttaaatca gctaaaagga tccatgacga cacctatacg atatagtttt
     3001 caggatacca aacaagtcta ttaatactca atgaaaatca aagagcaaac taggaagcta
     3061 gccgcaggtt gctcaaaaca ctgttttgag gttgtggata gaactgacag agtcagtatc
     3121 atatacctac ggcaaggtga agctgacgtg gtttgaagag attttcgaag agtataaaat
     3181 attcaggtga cgcatagata tagttaattg aagctttgtt tgaaatctga taaaataatg
     3241 atattactaa gttctaaaaa ctaaagaaaa gggaagatat gattacaggc gaattaaaaa
     3301 ataaaatcga tcagctgtgg gaaattcttt ggacagaagg aaacgcaaat cctttaacaa
     3361 atattgaaca gttgacttat ctcttattta tgaaagattt ggatagtgtc gagcttggac
     3421 gtgaaagtga tgctgaattt ctagggattc cttatgaggg agtttttcca aaagataaac
     3481 ctgaataccg ttggtcaact tttaaaaata taggagatgc tcaggaagtt tatcgtttaa
     3541 tgactcagga gatttttccg tttattaaaa atctcaaggg ggatacagat gatacagcct
     3601 tttcacgata tatgcgagaa gctatttttc aaataaataa acctgctacg cttcaaaagg
     3661 caatttctat cttagatgtt tttccaacta ggggattaga tgtagatttt gataatgaca
     3721 aacaaagtat tactgatatc ggagatatct atgaatatct gttatcaaaa ttgtcgaccg
     3781 caggtaaaaa tggacagttc cgtacacctc gtcacatcat cgatatgatg gttgagttga
     3841 tgcaaccgac tatcaaagat atcatctcag atcccgctat gggttctgct ggcttcttag
     3901 tatctgctag ccgttactta aagcgtaaga aagatgaatg ggaaaccaat acagataata
     3961 tcaatcattt tcataatcag atgtttcatg gaaatgatac ggatacgact atgttgagac
     4021 ttggggcgat gaacatgatg ctacatggag tagaaaatcc acaaatcagt taccttgact
     4081 cgctgtctca agataatgaa gaagccgata aatatacttt ggttttagca aatcctcctt
     4141 ttaagggctc acttgactac aattcaacct ctaatgacct tcttgcaacc gtaaaaacca
     4201 aaaaaacaga attactcttt ctttctcttt tcttgcgaac tttaaaacca ggtggacgag
     4261 cagcagttat cgtacctgat ggtgtccttt ttggttcgtc taaagctcat aaaggaattc
     4321 gtcaggaaat tgtagagaat cataagcttg atgctgtaat ctcaatgcct agtggtgtgt
     4381 tcaagcctta tgctggagtt tcaactgcta ttctcatctt tacaaaaact ggtaatggtg
     4441 gtactgacaa agtctggttt tacgatatga aagcggatgg tttaagtttg gatgataagc
     4501 gacaaccgat tagcgacaat gatattccag atattatcga acgctttcat catcttgaaa
     4561 aagaagcaga acgtcagaga acggatcaat ctttctttgt tccagttgct gagataaagg
     4621 aaaatgatta tgatttgtct atcaataaat ataaagagat tgagtatgaa aaagttgagt
     4681 atgaaccaac agaggtcatc ttaaagaaaa tcaatgattt agaaaaagaa attcaagctg
     4741 gcttggctga attagaaaaa ttactcaagt agggaggtgg ctgtatgaaa aaagtgaagt
     4801 tgggggaaat cttatctcta aaaaaaggaa agaaagccac tgtacttgct gaacaaacaa
     4861 ctctaagcca acgttatatt caaatagatg atttaagaaa taataataat ttaaaattca
     4921 ctgaaagttt aaatatgact gaagcactcc cagatgatat tctaatagca tgggatggtg
     4981 ctaatgcagg aacagttggt tatggattat cgggagctgt tggtagtaca attacggtct
     5041 taaaaaagaa tgagcgatac aaagaaaaaa ttatatcaga ttacttggga gtctttttgg
     5101 aaagtaaatc gcagtattta cgagatcatt caacaggtgc aacaattcct catttaaaca
     5161 agaatatatt acttgattta caattagaat tgctaggtat cgaagaacaa gagaacatta
     5221 tctgtattct taatacgatt aaaaggctta ttactaaaag aaaattgcag ttagatgaac
     5281 taaacttgct cg
//
LOCUS       DAVKGC010000116         5244 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKGC010000116 DAVKGC010000000
VERSION     DAVKGC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.116"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..186
                     /locus_tag="VW049_001806"
     CDS             <1..186
                     /locus_tag="VW049_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9432879.1"
                     /translation="EDAKNKKSYDRLAICYVRIGICRDNAKLIQKGFSLLELTEETSM
                     LSHLKKEVEIYYQAKER"
     gene            complement(475..945)
                     /locus_tag="VW049_001807"
     CDS             complement(475..945)
                     /locus_tag="VW049_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU9432880.1"
                     /translation="MRMPLKRPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVAVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQTGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(911..1717)
                     /locus_tag="VW049_001808"
     CDS             complement(911..1717)
                     /locus_tag="VW049_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9432881.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(1922..2563)
                     /locus_tag="VW049_001809"
     CDS             complement(1922..2563)
                     /locus_tag="VW049_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432882.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(2560..4668)
                     /locus_tag="VW049_001810"
     CDS             complement(2560..4668)
                     /locus_tag="VW049_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9432883.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(4720..5016)
                     /locus_tag="VW049_001811"
     CDS             complement(4720..5016)
                     /locus_tag="VW049_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9432884.1"
                     /translation="MIGEINHEKISVASLMVCSILASTSVLAVWVDGGQWNYGVGWSG
                     NFGYSDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGFE"
BASE COUNT         1620 a         1165 c          854 g         1605 t
ORIGIN      
        1 gaggacgcca agaataagaa aagctacgat aggctggcta tctgctatgt ccgcattggg
       61 atttgtaggg ataatgcgaa acttatccaa aaagggttct cactcctaga gctgaccgag
      121 gaaacttcta tgctgtctca tctcaaaaaa gaagtagaga tctattacca agcgaaggaa
      181 agataaaaaa gccgagggat ttcctcgact ctttacatat tatttgttcg ttagttctta
      241 ataccagcca ttgttaaacc agaagttttt agctctgcca agccacatag tgtgtttact
      301 actatgtggc catgtcacca tgaatcgccg aacaatttaa cgagaaagta tgacttttac
      361 gtttatccca actcaattat gacatttttt tcaaaagtca atatatctca ctttttcaac
      421 gacaagaaag aggctgccaa ttgaccaccc tcttattctg aacccatcac tccatcactt
      481 tttagcttca ttcgctttct tagcgactgc aatctggtat tcgacttggt cattcccctt
      541 acctgtacaa ccatgagcaa ttgtagtcgc tcctgtctga tgcgctattt caaccaattt
      601 tttagaaata agagggcggc tcaaggcaga taccaagggg tacttttgtt catagtaggc
      661 atgtgactgg agagctacta gcacataatc tgtagcaaat tcgtccttaa catcaatgac
      721 ataagattcg actgccccaa ccttaagagc cttatcatgg atgaaatcta agtctttccc
      781 ttcacccaca tccatacaaa ctgcaacaac atcatagtct ttcttcagcc aggtaatagc
      841 aactgatgtg tctaaaccgc cagaataggc caaaattact ttttctttac tcatgttctt
      901 ttccttcttt ctatttttca atggacgctt taaaggcatc ctcaatgagt ttgtctaatt
      961 ccccagactc cttcaacttt tgaatggttt tatcgactgc ctctttcaat tccttgctat
     1021 cttttttcat ggctaccgca taggaatcat cttgctcttt ttcaaaattg aggtctgcga
     1081 ttgctaaatc aggattattt tccacaaatc ccttggcaac gggttcttca aaaataacgg
     1141 catccacttg ccctgatttt aaatctgtga ttaaattccc atttttaggc agagatacga
     1201 gggaagaatt ttgtagcaaa tctttcgcca tcgtctcttg aatcgaacct ttctgcgctc
     1261 cgaccttttt ctgcgccaaa tcgttgacag actgataagt ggctaaatca gattttttga
     1321 caatgagttt attttttgca gtatagtagg gagttgaaaa atcaaacacc ttgctccgtt
     1381 catctgtctt agaaacacct gatatggcaa ggtcagcttt tcctgattga acgctagcca
     1441 gtacattgtc aaaactcata ggagatagtt ccaattcgac acctagttct gttgcgatag
     1501 ccttggctaa ttcgatatct gaacccacaa tctgattttt cccatcaacc actttttgat
     1561 attcaaatgg agcaaaatct ggattgaggg ctacaaccaa ttttcctttg gacttgatgg
     1621 cttcaattcc agccgattga ttgttactac aagcaaatag tagggggagc atcactaaac
     1681 caaacatcgt catcaacacc ttcttcatct tattcataac aaagcctcct ttatgtttat
     1741 tctttttatt gtataaataa tactcctata ctcatccttt gtcaagccta aattgaaaaa
     1801 attttcaatt tgaaacaaaa aaacctagaa atactacata aaatgtcatt cctaggcgta
     1861 tttatgccat ttttctctgg aaaacatgag tattcagcca gtcaaaagga gctgaatgaa
     1921 ctcattttcc ctcgcccaat tcaatgacac gatgacattg ttgggctaca taagcatcgt
     1981 gggtcacaat aatgactgtt ttcccttctc gattcatctc taagagaaac ttcaagacca
     2041 aatctctatt ttcaggatcc agcgaacctg taggttcatc ggctaaaatc agctggctgg
     2101 gttttaagat ggctctagca actgcaattc gttgttgttc gcccccagac aactcggaga
     2161 ccctttgatg caaagtagct gacaaaccta ctctctctaa aatctcttcc acctttttga
     2221 gcttgtcttt cttaggcaat ttcacatatt tcagcgccag catgagattg tactcgaccg
     2281 tttcatcatc aatcagggca aaattttgaa acagataaga gatatgttca cggattattg
     2341 tttgcgactt agcagaatta accgctagat ttgtctgacc aaaaatctca taccgtccgc
     2401 tataatcacc atctatcaaa cccaataaat ttaacaaggt cgacttccca ctaccactct
     2461 taccaacaat agctaccaaa tccccctgat caatcctgag agataagtta tccaaaatca
     2521 cttttccccc aatggttttg gtaatatttt tcaactcaat cataagatgc cccctttcaa
     2581 taactctact agacttcttt tctccatcct agaagctaag cctagcacaa atagtatatc
     2641 cagacatgta aaacctgcaa acagtagaag tggtaagaac gcatgggcaa agaaaatcaa
     2701 gactagaaga gggaaactat agcccagcaa gagcagaacg aggagaggac ggtagcgatc
     2761 gaccagtttc caccccataa acttcttggt aatgatatcc ctgcgcttca ataagaaagt
     2821 tgttactagt aagaagtagg aaatcatcat gctaaggaga ccaaacaaag caaagagtag
     2881 gttaaaattc cgaacagcat ctctataaga atccactttc tcttgttgaa tggcttgaat
     2941 agatgaaaat tttaaataat ttccatctga caatttctca actaactctg taatctcttt
     3001 ttgatgttga accgtatttt caattttaat cggattattt aagccagttg ttgacaggga
     3061 ggctttctca tcccacatca tatcagaatc attgaccaag ctaataattg gattggagag
     3121 attttccttt cgcttatcac tatatgggaa aaatgaccaa tctccttcat aataggcaat
     3181 ctcgacatcc atctcctcta tcgttcgttt ttgctgctct tcatacttca tcgaatgaaa
     3241 ggcaattaac ttccccaaga gctgattttt atcttcttca cctttcgtac ttgctggcat
     3301 caaaataact tttttaatac cggtatctgg tagcttgaat cccttgctct ttagaaaatt
     3361 gcgattggca tagtaaacat ccaccgtatc tgttaactga tattgctgaa tctgttctga
     3421 ttggacaaaa ttttttacag gaagactgct actctgcaca tagcccgcct gcgttttttc
     3481 taccaaatcc tgataaaatc gatagaaata atctgtagat ttccctgacc ctgctagctc
     3541 ttcttgccac agattatcat tgagtttgaa ggtttctaag gtcaggtaat taccttgact
     3601 tacccactgt tgctgataag caagttcttt gttttcttgt tctaaacttc tgcccacccc
     3661 aatcagtaag gccgtcagta aaatagttgt ccctattttc atcacataat tgaagataag
     3721 accaaatttg aaagatgaaa aacctttcag cagagagctg attgtcattt tttggattaa
     3781 gaggtaagtc aaccaactga taaagagata aagctgcaac agcaaaaaat gagacaacca
     3841 cagcatagga aacaaatctt ttggcttata atcaagcaag aaaaacacgc ctagattgat
     3901 cacaagagcc ccacctagga ggaggtaaag gttgcctttt acaacatcag ctaaaacagc
     3961 cctatcttga aaaccaagta atttttgtac cccaactctt ttcatctcca tcatcggttg
     4021 atacactgtc actaacacaa gaagcaaaat agccaagaca aaaacaatgg cagataaaag
     4081 caaatctcga tttatgactt ccactgcact tttgtaggtc ggctctagca aggtagcctg
     4141 gtctatcttg aaaaaatcgc tccatttctg tacaatccta tccttgtcca tctcttgtgt
     4201 agaagttatc gtatagcgac catttaaact acgagatgta tccttgatat aggtttgaaa
     4261 agtcataagc tgaataggtt tggcttttag aaaggtcgga atcgtaccaa gtttattgga
     4321 aatttcttta ttactataga ctccttcacc atctgtggta aaatcaagag aagaaatccc
     4381 aaactcttgg taggggaagg tatctttatc aaaaacacca gacttgacca cctcatcacc
     4441 actgtctgtt ttgatgatgg agactttata ctcctttgat acatcctcaa aaaatcgaag
     4501 aacagacgct gcaggttcgt taatatcttt caaatacaaa tccaaagaat ctacagtctt
     4561 tcccatttct tgaatccgaa ccacttctcg tgtatagttt acattaaaaa caaaaaaagt
     4621 ggtacacaga agcaggagta acatacagaa attactgatt tttttcataa aagtgcctcc
     4681 actacagttt tccctctcca aataggagag ggaagaattt tactcaaatc catagaaata
     4741 ttccattccc gttggtggga atttgaagat tgaagcccga gcccatgcct cagcttctgc
     4801 acgatcctta gagaatttat ttccatcttt aacagttgct gtatgagagc gagtagaatg
     4861 taaataatca gaataaccaa aatttcctga ccatcctact ccatagttcc attgaccacc
     4921 atcaacccat actgccaaaa cacttgtaga agcaagtata ctacatacca ttaaagatgc
     4981 aacactaatt ttctcatgat ttatttctcc tattatcgag agctattccg tagaaacagc
     5041 tatagtaatt tgtcaacaag tatcgtagtc cattggactc accttccttc tatctcattt
     5101 catttttaaa caacgaactt actcttaact aggagaatag ttctcttctg tatcaggtct
     5161 ttgaacaacc agagtagtat taatcacact ctaactatta ctcaagaatc agcccaataa
     5221 acaatatgga tagcgctttc aaat
//
LOCUS       DAVKGC010000117         5190 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKGC010000117 DAVKGC010000000
VERSION     DAVKGC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.117"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            99..476
                     /gene="mscL"
                     /locus_tag="VW049_001812"
     CDS             99..476
                     /gene="mscL"
                     /locus_tag="VW049_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9432885.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(518..1612)
                     /gene="hemH"
                     /locus_tag="VW049_001813"
     CDS             complement(518..1612)
                     /gene="hemH"
                     /locus_tag="VW049_001813"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9432886.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGMRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESQDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            1767..2990
                     /gene="pepT"
                     /locus_tag="VW049_001814"
     CDS             1767..2990
                     /gene="pepT"
                     /locus_tag="VW049_001814"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9432887.1"
                     /translation="MTYPNLLDHFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(3026..3655)
                     /locus_tag="VW049_001815"
     CDS             complement(3026..3655)
                     /locus_tag="VW049_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432888.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            3620..3841
                     /locus_tag="VW049_001816"
     CDS             3620..3841
                     /locus_tag="VW049_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432889.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNIL"
     gene            complement(3978..4187)
                     /locus_tag="VW049_001817"
                     /pseudo
     CDS             complement(3978..4187)
                     /locus_tag="VW049_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad protein"
     gene            complement(4308..4450)
                     /locus_tag="VW049_001818"
                     /pseudo
     CDS             complement(4308..4450)
                     /locus_tag="VW049_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262445.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(4484..4624)
                     /locus_tag="VW049_001819"
     CDS             complement(4484..4624)
                     /locus_tag="VW049_001819"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9432890.1"
                     /translation="MLEITLKSPYQFAHILFQSTIVPHGGHYHFIPESDLSAGELAAA
                     KV"
     gene            complement(4668..5190)
                     /locus_tag="VW049_001820"
                     /pseudo
     CDS             complement(4668..5190)
                     /locus_tag="VW049_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         1497 a         1019 c          950 g         1724 t
ORIGIN      
        1 atatttttag aaaataatgc tccatttatg agccttttaa ttgccttcta taatgattta
       61 ggatacaatg aaacacgaaa cattgaaagg aaaacagtat gttaaaaaat ctaaaatcgt
      121 tcttgcttcg aggaaatgtt attgaccttg ctgtcggtgt tgtaattgcc tctgcttttg
      181 gtgctatcgt tacttcactt gtaaacgaca ttatcactcc tcttatttta aatcccgctt
      241 tgaaagctgc taaagttgaa cgtatcgctc aactttcttg gcatggagtc ggctatggta
      301 acttcttaag tgctattatc aattttatct ttgtgggtac cgccctcttc tttattatca
      361 agggcattga aaaagcacag aaactgactg gcataaagga agaaaaaact gccgaaaaaa
      421 aaccaaccga attggaagtc cttcaagaaa taaaagctct ccttgagaaa aaataatcga
      481 ttaaaaggat ctagcgcaaa gctaaatcct tttttcttta ctctttgggt aacttgcctg
      541 ctttttcaag catttttttg ataagataag gcatcttaac attctcccgc ccttttttct
      601 caatcagttt tttcacaaat tctggcattt gtaaatcttg agattcggct aagatatttt
      661 tagtctcatc tgaaggcact aactcatcaa aggtttcttc ttctgggaga aattccttaa
      721 actcttgatt ttcattgact ctaactgtat tgaccaaggc atcaatcaaa cgagaatctg
      781 tatttggcat tggtggacga tggtagttta ccccaaattc ctgacataaa tcataacatt
      841 ccacatcatt gtcaaacaag acctcaatat gctcactgat gaaactaata ggtacaaaaa
      901 tataatggtc tggatgttct gtctgttctc tgagatactc caaaacatct ggtttaatcc
      961 atggaatacc aatatcactc tcactctgcc aagtgttggt atattgttct gaactcaagc
     1021 ccagtttttc agcgactagc ttgctatttt cgaaaatttg gtctatataa ggatctccaa
     1081 agtccaaggc aaaaataggc acactatggg ctgaaaagat gactttaaag ctgtcttgct
     1141 ttacttcttc ttttaaaatc ttagcaattt catctgccca atagtttaag agcgcttctt
     1201 cctgatacca gtccttaatg accaaaaatt gaatttgttt gctttctaaa aatttttcat
     1261 agcccatgac agagtagaaa gaataatggg gctccaaaat caagcaaata cactgttcaa
     1321 ttccgtctgc ttccatttga ccgatcacat ctggaataaa gggactggaa aacttattag
     1381 caaagtagac actgtattca ttccccagcc tagcttctac caaggcaacc tcttgacggg
     1441 taattttttg cagaggcgtg cctccaattc gtacataatt atcatagaga gtctgaatct
     1501 cgtggtcttg gggtctcatc ccacgacgaa tatttgtgaa aaaatcagct acaccttcaa
     1561 aggtaatctc ttctggtgaa ccgaaggtca tcattaaaat tgcttttttc atgactgctt
     1621 ccttgtgatg tttttatcaa gtttatttta tcattaattc atcgataagt aaacgctagc
     1681 atagggacat tctcaatctt ctgtcttttg tttttctctt ctttctatga tacaatggaa
     1741 aaaataaatt caaaaggagt ttttttatga cttatccaaa tctcttggac cacttcttaa
     1801 cctacgttaa ggtcaacacg cgctctgatg aacactctac tactactcca agtacacaga
     1861 gtcaggttga cttcgcaaca aatgtcctaa ttcctgaaat gaaacgtgtt ggactgcaaa
     1921 atgtttacta tctaccgaat ggttttgcta ttggaacctt gccagccaac gatccgtctt
     1981 taacacgtaa gattggtttt atatcgcaca tggatactgc tgattttaat gctgaaggag
     2041 tcaatccaca ggtaattgaa aactacgatg gtggtgtgat tgaactaggg aattctggtt
     2101 tcaaactcga tccagctgac ttcaagagtc ttgaaaaata tccaggacaa acgctcatca
     2161 caacagatgg aacaaccttg ctaggtgctg atgacaagtc aggaattgct gaaattatga
     2221 cagccattga atatctaact gctcatcctg aaattaagca ctgtgagatt cgtgttggtt
     2281 ttggtccaga tgaagaaatc ggtgttggtg ccaataaatt tgatgcagaa gattttgatg
     2341 tggattttgc ctacactgtt gacggtggtc cactaggtga acttcagtac gagactttct
     2401 cagccgctgg tgctgaattg catttccaag gccgtaatgt ccaccctggt actgccaaag
     2461 ggcagatggt caatgccctt cagctagcaa ttgattttca taatcaactt ccagaaaatg
     2521 accgacctga gttaactgaa ggttaccaag gtttttacca tctaatggat gtgacaggta
     2581 gtgttgagga ggtgcgtgca agctacatca ttcgtgattt tgaaaaagat gcctttgaag
     2641 cgcgtaaagc atccatgcaa tctatcgctg ataagatgaa tgaagaactt gggagcgacc
     2701 gtgtcactct caacttgaca gaccagtact acaatatgaa agaagtcatt gaaaaagata
     2761 tgactccaat taccattgct aaagccgtta tggaagatct aggtatcacg cctattatcg
     2821 aaccaatccg gggtggaaca gacggctcta agatttcctt tatgggaatc ccaactccga
     2881 atatctttgc aggtggcgaa aatatgcacg gacgttttga atacgttagc cttcagacta
     2941 tggaacgtgc agttgatacc atcattggca tcgtagctta taaaggctaa aaagacgagg
     3001 tagctcagct acttcgcctt tctttttatt ctactggttt ttcttgattt ccagtagttg
     3061 tagaagattc tgttgtttca ttttctgaag ttgattcagc aggtttagaa tctcttgtat
     3121 tgcttggttt gttttcgtcg ctagcagttt caatgttaga ttctgcagtt gcgtttggtt
     3181 ggttctcagc actggtgtta tcactatttg cttcagcatt tcttgctgga cttgtttctt
     3241 cacttgcgct agcttttgac tggatttgat gattcaaaac tagaatagct tttgtcgatt
     3301 caagtaaagc tgttttgtct ttactcttag cagaaagttg atctaataat gcatccacct
     3361 tatcaaagtc cgcatcagat ccattattac tttctaaata agagtgaagc gacatgagaa
     3421 tatcgtagag tttttgatag agtacaagtg tctgagaatc ttgctcagca ttttcctttt
     3481 cttgttgaag ggcgctagcg atacgagtca aaacatcttt tacctgactg tttacttcat
     3541 ccaagtctgc atcagccttg tttgtggcag cttttagatt ttctacttct tctgccaagg
     3601 attgtctgat tccttcttca tggatttgtt ccaagagttg atttgccttg ttcaaaaaac
     3661 tttctacttc ttccttgcta tctgtcgcag attattggtt gctatctacc atgtactcct
     3721 aaaacaggag agttataatc caagattaca aggccttaca gaaataagaa atccagataa
     3781 gacaatgttc gtccaagacg ctattcgctt cgcacagcag cacggattca atatacttta
     3841 attttaaagt ttaggtgtca agacctcttt ttagtgtgcc caaaatttag agaagtaatc
     3901 aatcaactaa cttttatttt tttcaaactt tcagtaaact gacctaaagc taactcaatc
     3961 tgtctttgtt cgataggctt gtctttgtag atgcttctgc tatcagctag aagttgatct
     4021 acttttgcca agactgcctt ctcatcaaaa gttccaggtt gatagttgga ttgcagggat
     4081 ggaatcttgt ttttcaaagc cgcttcatat cccttagttt gaaccttgat gtagtgattg
     4141 tggtcgccac gaggaatcac aaaaccttct gaatcttcac ttataattcg attggcatca
     4201 aaaccatgac catcttcttc ctcatggtgg acatgtagtg acggattact taatacagaa
     4261 ctagaagaac ttcctacctt ttccgtgtta gagtgtgatg ggggattgtt aagagatgac
     4321 ttaggaatat agtgatagtg accccatgtc ttactatata agcatcacct gtatctctga
     4381 caatatcatt agggttaaag acataaccat catctgctgc agaaacacca ttattcggtg
     4441 tcaccgacaa agattgactg agagctgtag tattctctga taattatact tttgcagctg
     4501 ctaattcacc tgccgacaag tcactctcag gaatgaaatg atagtgacca ccatgtggta
     4561 ctatagtaga ttgaaataga atatgagcaa attgataagg ggattttaaa gtaatttcta
     4621 acaatgattt agaaactatg atgtgctatt ctaaattcaa ctcactatat ataaccatca
     4681 tcggtagtat aacgtccctg taattttgct acagatactt ctgcactagc tcctttatcg
     4741 tctttaccat gttcttgttt ttggcgattg atttcatctt ttattcgtac attttctgca
     4801 tgagcttgat ctttaaggta aacataatac tttccatcta ccttaataat atatcctccc
     4861 ttaacctaac tgacgatatc ttgatctttc ggctgatagt tgggggcttt cattaatagc
     4921 tcttcactaa agagcgcata caaaggaact ttaccattat agcagtgata atgatcgcca
     4981 tgagaagtta cataaccttg atctgtaatc ttaataacaa tttgttttgc ttgaattcct
     5041 tctttttgac taacctagtc tggagtcaaa ttttcagtct tcttagtgtc tttattactg
     5101 tttacatatg aaacacgatt tttatctgta ttggcctgtt agctatgttg gttcagagca
     5161 taaacacaca gacttaagga aaggataaca
//
LOCUS       DAVKGC010000118         5022 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKGC010000118 DAVKGC010000000
VERSION     DAVKGC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.118"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(58..672)
                     /locus_tag="VW049_001821"
     CDS             complement(58..672)
                     /locus_tag="VW049_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU9432891.1"
                     /translation="MPTALGYVSIGLACGIIGVPYVTLVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVTAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFALILSSVVTGKVG
                     SLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAVLRLVF"
     gene            743..820
                     /locus_tag="VW049_001822"
                     /pseudo
     CDS             743..820
                     /locus_tag="VW049_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(1127..2353)
                     /locus_tag="VW049_001823"
     CDS             complement(1127..2353)
                     /locus_tag="VW049_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9432892.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(2425..3090)
                     /locus_tag="VW049_001824"
     CDS             complement(2425..3090)
                     /locus_tag="VW049_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9432893.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(3158..3355)
                     /locus_tag="VW049_001825"
     CDS             complement(3158..3355)
                     /locus_tag="VW049_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU9432894.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            complement(3646..4509)
                     /locus_tag="VW049_001826"
     CDS             complement(3646..4509)
                     /locus_tag="VW049_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9432895.1"
                     /translation="MIERMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTSLFE
                     LRFQQYKELID"
BASE COUNT         1724 a          995 c          909 g         1394 t
ORIGIN      
        1 atcatttaca ccattatatg atatatatat ctgtgatagg ttttctttat ggctgattta
       61 aaagaccaaa cgcaagacgg caatcaagac tactccaaag agaactgttc cgactagatt
      121 gcggtagcga aaggctaccc aagctgttgg aaagacggct aagaagtcta gccatttaat
      181 ttgaggaagg ctcccaacct tacctgtcac tacgcttgaa agaatcaagg caaagatttc
      241 tggatttggt agcaggccac ccagagccgt tccgactact gtccccacaa accaagccac
      301 atagctgtta agattgtttc cgtgcatcca cataggattt accttgtctg tatgggctaa
      361 ttcgcccatc aaaacgccat aggtctcatc tgtcaagata ctagacatac cgatattgta
      421 ccaaagactg gtatgacgga aataagtcga tgcgtgtaaa ctcaacaaaa agagacgcaa
      481 gttgattaga aaaaccgtca tagcaatagc tgtcacagga gcttgaacca caatcagtgc
      541 caacatggca aactgggcac tcccagcata aacaaagaga ctcatcaagc ccatctcaac
      601 aagtgtcaca tagggcacac cgataattcc acaggccagg ccgatactga catagccaag
      661 agccgttggc atggctgcct gcgccccctc ctaaaatcct ttttctttca tctttctcct
      721 catattgtct taataatact caatgaaaat caaagagcaa actaggaaac tagccgcagg
      781 ttgctcaaaa cattgttttg aggttgtgga tagaactgac gaagtcagta accataccta
      841 cggcaaggtg aagctgacgt ggtttgaaga gagtttcgaa gagtacaagt aggctgaaaa
      901 gaatccagcc acagcatgga ctattatata gcagattgaa ataagatgag aacaaatcga
      961 ttgggaaagt aaaattaatt tctataaatg ttttagcaat tgtttcgtac tattttagat
     1021 tcagtctact ataacacatt cagaaaagag aaaaaagtct gttgattttg accatcataa
     1081 aaagactggc aatccagtct caaacatatt ttatagaaat tctccactaa atactttcac
     1141 gaatattcag aagcatgaca aaggcaacta gaagaaatag caataaaaca aagctaactg
     1201 ccagagttcc aaagctggta gcaatggtta ccaaagctat tgtaaataag ctaggtaaga
     1261 caaccgtaat ggcaccgata gaagattgaa ctgctcccat tgactcctca ggtatttgtt
     1321 taaagacgag ttcttgcaat ctaggagaga gaatacccgc gaaaaaggca tccaaggtac
     1381 taaagatgag aatccagtca aaacgaactg tggcaaatcc tactagaaga agcaactgga
     1441 taacaagtga ggcatagaga gctgttttta tggaaatggt atgttgcaga tagccactca
     1501 taagacttcc caaaataagg gctgataatt ctagtgtggc taacaaggca agagattgac
     1561 cagtttgtaa attcaaaaag ggctggttcc ttaaaaatag agtggaaata ggaaccgtaa
     1621 catttatcac tgcttgacta gtagagataa taaacaaaac caagagcacc ttattcatat
     1681 tccatatcaa tttcgatgat tggagcaaat gctggcaaaa ggattttaca gagagtcctt
     1741 cttgatagct aatcgttttt tctactttca agaggtcagt ttttatgaag aggataccta
     1801 aaaatgcgat taaaaaggta agagcgttca gtaaggaaat aaactggatg gatagaatgc
     1861 ctagtaagac tcctcctagg atattactga ttgttttcac taaactaaca gttgactgtt
     1921 taaatccaat agcttctgcc agatggtctt gcccaataat tctaatgaaa atcggagtga
     1981 gcatggcgcc tgaaaaataa ctcaatgtgt cagacaagag gttaagcaga caaataaagg
     2041 ttactagcaa caaggaaaaa gactgccctg aaagcgatag ggacactata gagtaaagca
     2101 aaaattttgt aaaactaatg actgtgtatt tcaagacacg atgatgttga aaatccgcca
     2161 aaactcccag aaagatttgt agaacttggg gaagggtttc tgaaatcgtg ataagtaaaa
     2221 tcgccaaagg ggcaaaagac gcatctgcca cataattcag gaaggccaga taaaaaatcg
     2281 tatccccaag cgttgaaatc cactggttga tagttaattg cctaaaatct ctattttgaa
     2341 gaaatacttt catcacaact ccttcttaag ttcaaatggg aatctttccc cagaaataga
     2401 ccgcgatact actaacaacc aaaattacag taacatcaaa agctgaccaa tgccattgta
     2461 gactatatgc agtccaatag gccaataaat tgactttgtc attctaaata agactgcaaa
     2521 tataagacct ccacccatat agaagacaaa gtctgtcaag acccaaccgt gattactaat
     2581 gtgcgagacc ccaaataaaa cagcggaacc aagtacatct agcccccatt tctttccttt
     2641 ttccagagca gtcatcacta atccacgata aatcatgtct tcaaaaatgg gacctgcaat
     2701 cacaggataa aaaaaataca tcaaaaatgc cgtagctcct gtaaaagtag gagcagcatg
     2761 ttgataagaa atttcatttc gagtaggtgg gaaaagaaaa aaggtgacaa aattccaagc
     2821 aacaaaagca agcagagcta ggaaggaata gaaaagatag gatcctttaa actttctact
     2881 attgattttc tgccatttcc ccgaccaaat catagcaata agagcaaata aaaccacaag
     2941 aaaattcaac atcatatccg acagataata ggcaaagtca gatagcccag caacaaggtc
     3001 gctgcgtaaa actaaaacac tgaacttctg gtcagcaata actagtagaa aaactataat
     3061 aaagtagcgg tgtgagatta tctttttcat tctattttct cctatactat gaaataaaga
     3121 cagtccctac ccaagaaaaa ggcaaggact ctgaatatca ataatggcga cgattaacag
     3181 tggaagaatc atggttaaaa tatttcttcc agaaattagt gattgaccaa gacgttccta
     3241 aacctcccca agcaccggct gggataacag cattattccc aaattcccct ccataaactg
     3301 tctccaactc agcttctgtc aattccattg tttctgcaaa ttgtaaattt aacatctttt
     3361 acactccttc aattatcttc atttgtaaac cacttctgcg acctaggatt tgcttcaagt
     3421 gctttacaag tacagtataa cacgaaaatt ggcttatttt aaaaaatcgc atatttgata
     3481 ttttttctta tagaaatttc ttatttgcga ttttgataga tttgattatt tccctggtat
     3541 aataaagtta ttactaacaa ggaggaatat aatagatgaa gaaaagaaaa atccaactga
     3601 tactcctact tatttcggag tgggttattg tcattccatt tttaactaat ctataagttc
     3661 tttatattgc tgaaaacgca attcaaaaag ggatgttaat tgtggatttt ctaatacctg
     3721 cagagattgg ataaagcgtt caatctcttt ttgattgctt ccctttgttt gaagaaagac
     3781 actcatcttc tttaaaaatt gccacgatac tttttcaaaa acatcatacg gtcgtaacat
     3841 cctctccaac tcggcttcga agattgggat gtaggagaaa agttttcgct ccatgagttc
     3901 tgataagata tttaagagtc cttgcttcat atacaatcga ttgtgtacta actctttaaa
     3961 ttctttggat ttttcgagta aggaggttga taaaaaaatc agatcttgat tgctcaagaa
     4021 gggcatggta ttgcaaaaga gatagagttc aaaccaggtc caagactcga tagcatagag
     4081 ataggtggtc aaaaactcgc tatcctcctc tgctagtggg tagcttttat ttagtgaatg
     4141 gatggcatct ttaatcacga tggcattcaa acgacgatag gtctgcgcca tctgttcttg
     4201 atcgacttcc tccaatagct gctctaaagc agctatatcc tgatgggcaa agcgattcac
     4261 aacctttcga ccgattcgca tatgtggaga ttcttgatag ttgttgagct tgtgcccaaa
     4321 ctcatcaaag gtcacattta taccttggat agctagaatc aacttatccg cagacagcat
     4381 agactgccct agttcaaact tggacaactg agaagctgtt agaccctcac aagccacatc
     4441 tgtctgctta agctttctcg ccaaacgtaa ttccttgtaa aattccccca gttccattct
     4501 ctcaatcatc tgaccacctc ctagcttttg catattatat cattatttac aatcatttgt
     4561 caaaatattc tgatatttaa gagggcaaaa aagccagcct taagctgact ctaaaaggac
     4621 cgagccttgt acaatacaga actcaatcct cgttctaaat agaatgtcta acttttttgg
     4681 gcagtacacg agcttgggga acagccactg gaacgatgag gtgtgagctc aaaatatcct
     4741 ccagttatgt ttttcctaat agtataccgg aagagtgaaa ggattttata atggagcggt
     4801 tacaaagaac ctactttcta ttaaacagta tactatgaaa atgtgaaaat ttaacatttt
     4861 tttgtacaaa ttttataaat tattgccttt ttaatatcaa tagttaatct cttatccaga
     4921 tcccccttgt gtaaacttta tctttataag cttcaaggcc cctatcccat ctatttgcaa
     4981 caattagatc actttgtttt gtaaatagtt caaaattctt tt
//
LOCUS       DAVKGC010000119         4859 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKGC010000119 DAVKGC010000000
VERSION     DAVKGC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.119"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            63..665
                     /locus_tag="VW049_001827"
                     /pseudo
     CDS             63..665
                     /locus_tag="VW049_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            816..1184
                     /locus_tag="VW049_001828"
     CDS             816..1184
                     /locus_tag="VW049_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9432896.1"
                     /translation="MAVGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKAK
                     NMENDKKFIDFIISQEVQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIK
                     NKSDIVKKYNEVFTDIQSKQ"
     gene            1259..2287
                     /locus_tag="VW049_001829"
     CDS             1259..2287
                     /locus_tag="VW049_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432897.1"
                     /translation="MNITIPKGSLFTLLGPSGCGKTTLLRMIAGFNSIKDGEFYFDDT
                     KINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLKQKKVPKEELIQQTNKYLELMQI
                     AQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEPLSNLEAKLRLDMRQAIREIQH
                     EVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNI
                     IPANLEKRSDGAYIVFSDGYALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTISD
                     SVYLGLNTEYFIETGFASKIQVSEESTFEEELQKGNRIRLRINTQKLNIFSADGSQNL
                     IKGVNHGT"
     gene            2277..3929
                     /locus_tag="VW049_001830"
                     /pseudo
     CDS             2277..3929
                     /locus_tag="VW049_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4032..4724)
                     /locus_tag="VW049_001831"
     CDS             complement(4032..4724)
                     /locus_tag="VW049_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9432898.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1516 a         1046 c          841 g         1456 t
ORIGIN      
        1 tgttttaaaa acgcttacat tgttttaaaa taaacttaaa taacacaacg gaggtatcca
       61 tcatgaaaaa gaaatggatg tattatgctg cttgttcttc taatgaatct gccgatgaca
      121 gttcatctga taaaggagac ggcggttcgc tagtcgttta ttcaccaaac tcagagggct
      181 taattggagc aactattcct gcctttgaag aaaaatatgg tatcaaagta gaactgattc
      241 aagctggtac tggagaactt ttcaaaaaac tagagtcaga aaaagaagtt cctgtagctg
      301 atgttatctt tggtggttct tatacacaat atactaccca cggagaactc tttgaaaact
      361 atacttcaaa agaaaatgat aatgttatca aagaatatca aaacacaact ggcttctcta
      421 ctccttatac actagatggt agtgttttaa tcgtccaccc tgatttaact aaaggcatga
      481 acatcgaagg atataacgat cttctcaaac ctgaactaaa agaaaaaatc gcaactgctg
      541 acccagcaaa ctcttctagc gcctttgctc aattaacaaa tatgctacaa gctcaaggtg
      601 gttacaaaga tgataaggct tggtcttatg taaaagatct tttcacactt attgatggca
      661 tagtgaaatg aaataagaac aggacaaatt aatcaggaca gtcaaatcga tttctaacaa
      721 tgttttagaa gtagaggtgt actattctag tttcaataca ctataaaatc ggttcaagtt
      781 catctggtgt ctataaagca gtcgttgatg gagaaatggc tgttggtctc tcttatgagg
      841 atccagcagt taaactctta aatgacggag ctaacattaa agtagtctat ccaaaagaag
      901 gaaccgtctt cctacctgct agtgctgcta tcgttaaaaa agctaaaaat atggaaaatg
      961 acaagaaatt tatcgatttt attatctctc aagaagtaca agatacactt ggtacaacca
     1021 ctactaaccg tcctgttcgt aaaaatgcta aaacaagcga aaacatgaaa ccaattgaca
     1081 aaatcaaaac actcactgaa gattatgatt atgtcatcaa gaataaatca gatatcgtta
     1141 agaaatacaa cgaagtcttt acagatatcc aatctaaaca gtaaaaaagg ttcactatga
     1201 gtgagatcaa aattattaac gccaaaaaat ctaccacgat gtccctgtta ttgagaattt
     1261 gaacattaca attccaaaag gaagtctctt tacccttctt ggaccttcag gatgtgggaa
     1321 aacgaccctt cttcgtatga ttgcaggttt caacagtatc aaagatggag aattttactt
     1381 cgatgataca aaaatcaata atatggaacc cagcaaacgc aatatcggga tggttttcca
     1441 aaactacgct attttcccac atttgactgt ccgagacaac gttgcttttg gtcttaagca
     1501 aaagaaggtt ccaaaagaag aattgattca acagaccaac aagtatcttg aactcatgca
     1561 aattgctcaa tatgcggatc gaaagcccga taaactcagt ggtggacaac aacaacgtgt
     1621 caccttggca tgcgccttag cggttaatcc aagtgttctc ctcatggacg agccacttag
     1681 taatctggag gccaaacttc gcttggatat gcgtcaagcc atccgagaaa tccaacacga
     1741 agtgggaatt acaactgttt atgtaaccca cgaccaagaa gaagccatgg ctatttcaga
     1801 tcaaattgct gttatgaaag atggggtgat ccaacaaatc ggccgaccaa aagaactcta
     1861 tcataaacca gctaatgagt ttgtggcaac ctttatcgga cgcacaaata ttatccctgc
     1921 caatcttgaa aaacggagcg acggcgctta tatcgtcttt tcagatggct atgcccttcg
     1981 aatgccagct cttgatcagg ttgaggagca agctattcat gtaagcattc gtcccgaaga
     2041 gtttatcaaa gatgaatctg gagatattga aggaactatt agcgatagcg tctatcttgg
     2101 actaaatacg gagtatttca ttgagacagg ttttgcctca aaaattcaag ttagtgaaga
     2161 atcaactttt gaagaagaac tacaaaaagg caatcgtatt cgtctacgaa tcaatacgca
     2221 aaaattaaac atcttttctg cagatggttc ccaaaacctg ataaaaggag tcaaccatgg
     2281 aacgtaaaaa actaaatatt tggacagcct cctctttctt cctctttctt acctatcttg
     2341 tctttctcgt ttatcctatc attaccgtgc tcaagcaagc acttatacat gaaggacaat
     2401 tctcactagc taattttgtc actttcttta gtaaagccta ctactctgag acactagtca
     2461 acagtttcag ggtttccatt accgctactg tcacttcctt agttgtagga accctattag
     2521 cttatctctt ctctatgtat gacttcaagg ggaagaaatt tctacaaata ttgattatca
     2581 ttgcttccat gtcagctcct ttcgtaggag cctactcctg ggttctcttg ctgggacgaa
     2641 atggggtcat cactaaattt ttgacaaatg cccttcatct tccagctatc gatatttatg
     2701 gattcaaagg aattgtactt gtctttacac tgcaactatt cccactggta tttctatatg
     2761 ttgctggaac aatgaacagt attgacaatt ctctacttga agcagctgaa agcatggggt
     2821 ccttcggatt taaacctatc gtaacggttg ttttacctct tctagttcca accttactag
     2881 cagctccttg cttgtattta tgagagcatt ctcagacttt ggaacgccta tgttgattgg
     2941 cgaaggatat cggactttcc ctgtcctgat ttatacccaa tttattagcg aggttggagg
     3001 aaattctgct tttgcaatta tggcgattat cattgccttg gcaattttcc ttatccaaaa
     3061 acacattgca aaccgctaca gtttcagcat gaatctgctc catccaattg agcctaaaaa
     3121 aactacaaaa ggaaaaatgg ctgccattta tgcaacagtc tacggaatta tctttatctc
     3181 tgttttacct caaatctact taatttatac ctctttccta aaaacatcag gtatggtatt
     3241 ttttaaaggt tattctccaa acagttacaa gctagctttc aatcgtatgg gatctgctat
     3301 tttcaatacc attcgtatcc ctttgattgc cttagttcta gttgttctat ttgcgacatt
     3361 tatctcctac ctagccgtta gaaaacggaa tttgtttaca aacttaattg acagcctcag
     3421 tatggtacct tatattgtac caggaaccgt tctagggatt gccttcattt cttccttcaa
     3481 tactggtcta tttggaagtg gatttcttat gattacaggg actgctttca tcttgattat
     3541 gtctctatct gtcagaagat taccgtatac tattcgctca tctgttgcta gcttacaaca
     3601 aatagcacca agtattgaag aagctgctga aagcttagga agtagtcgtc tcaatacctt
     3661 tgctaagatt acaactccaa tgatgctatc gggtattatt tctggagcca tcctatcttg
     3721 ggtcacactg atttcagaac tctctacttc tatcctctat cctcctctac aatgtcaaaa
     3781 caagaacaat gactgtagct atttatacag aggttctcag aggaaattac ggtgtagccg
     3841 cagccttgtc aactatcctg actgttctaa cagtaggttc cttgctcttg tttatgaaaa
     3901 tctctaaaag caatagcatt acactttagt tttccacatc aaaaacagcc gaaaggagta
     3961 ccctcggctg ttttttctta tcttgatatt cttatcaaat ccctagctca tggcaagcaa
     4021 gtctagactg attaaataga gccttcttcc atcttctcac gtcctagctc tcggtaacta
     4081 cggtcaccga caacttcaac cctaaactga ccgtcctcat attcaagaat cgtcacgcta
     4141 ccattatcta gaccatgcgg atgcatgcca ttaatcagat aaacaatggt tccaatagtc
     4201 attccatggc tgacaacaag ggcgttacct ccaccttgat cttccatttc ttttgcaatt
     4261 atttcaaagc cttccttgat tcggccactg agtttttccc aaccttcagc ccaaccagct
     4321 gtatcgacct ctaccaagcc ctcagccagt tcagcataag acaattggtg aacgtggtcc
     4381 acattaaaga tacgaggaat aatgcccatg aaaagatcgc catcataggc tccatcaaaa
     4441 ctaccgaaac accattctct gatacgcttg tccatgcgat aagggatttt cccctgcaag
     4501 ccaagttctt caagtataat tcccatggtc tgaatggtac gaccagaatc actcgaataa
     4561 gcgcgctcaa actgtagatc agattctcgc aaaccgattc ctaactcttg aatccctcgt
     4621 tcaccttcag cagttaaggg agtatcgctc caaccttgcg cgcgaccaat cgtgttaaac
     4681 atggtcttgc catgacgtac caaatacaat cgtactttta ccattttccg tcctccgttt
     4741 gcttctatta tatcatgaat gataaaataa aagccaccca tacaggcgac ttttgaagga
     4801 gattattatg aaaaagttta ggagttcatt aaataaagat attagatgaa aatcaaatt
//
LOCUS       DAVKGC010000120         4816 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKGC010000120 DAVKGC010000000
VERSION     DAVKGC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.120"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            346..531
                     /locus_tag="VW049_001832"
                     /pseudo
     CDS             346..531
                     /locus_tag="VW049_001832"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1142..1445
                     /locus_tag="VW049_001833"
                     /pseudo
     CDS             1142..1445
                     /locus_tag="VW049_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1438..1686
                     /locus_tag="VW049_001834"
     CDS             1438..1686
                     /locus_tag="VW049_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432899.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            1782..2000
                     /locus_tag="VW049_001835"
     CDS             1782..2000
                     /locus_tag="VW049_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432900.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(2368..4692)
                     /gene="pflB"
                     /locus_tag="VW049_001836"
     CDS             complement(2368..4692)
                     /gene="pflB"
                     /locus_tag="VW049_001836"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9432901.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1547 a          876 c          885 g         1508 t
ORIGIN      
        1 gttttacata tagtatttag cgcgacataa tactgaagtc tctctccaaa aaacggttat
       61 gcttctttga ataggacgtt ctcacaagaa aagcatcttc acgtttcaac ttcatatggc
      121 tcaaaaacaa tcaattgatg ctaaaacctg tacctagatg tttcggttca taaaaccatg
      181 aaactgtaaa agtggatgaa attgatagcg atagtcaaat caagaggcat cataactcta
      241 aaaagtcaca atatataagt tcatcctcgg aaaaatatca ttctaattgt tgaaatggat
      301 atataaacag gggtattaac attaaatgct catgaaacaa cgaatacagg tatcaaaact
      361 atgacaaaac aagtccctaa atttactaaa gacactgctc aactttacac ctgtaaatgg
      421 ttgttgtata ataaagttac aaagatgtac gaccacactg ttgtaaatca tagtgttcgc
      481 gaatatatta ctgatagcat ttctacaaat acaattgaag aggctggata ggctttaaac
      541 gaaatatgtc agtgttttgg cattcctagc cttaatatca tttaaagaat tgtatagata
      601 aaattttttc caatacagag actcgtaaca actgcttcat ttttctacca acatatttag
      661 gaacaggata agatacaaga ggattaatcc ataaatcagt tctataccaa tctaagataa
      721 ataagctaaa aaacaattga taataagcaa atagattcca aattttctct atctgctcat
      781 tttaataaac aatactagtg taactatcct tccagtcaga agcttgtcaa atcacaccga
      841 aaattcttct aaaatttatc tcgttaggca atcaagcaaa aactcgacga tagtacaaac
      901 attatcatac aggattgact tcctaaatta tatactttag taaggttttc gtataagaaa
      961 aaagattcat tttacatttc taaacattct tttctaagat gaaaaacaga atttttcgat
     1021 tgtgatttaa agcaacaaga agattttcag tatcatccta tagatacgag ctaattaaga
     1081 aaaactacat tttcgaatat aaactacaat aatataaact aaattttata ggaggaagac
     1141 aatggattgg tacgattata tgatacaggc atccaaacaa tcacaattca acgcaagcca
     1201 ttggtttcgc tatttgcgaa aagttatttt tgaagactat tcttatttaa caaaccaaga
     1261 tgtatttaac aaaccaagat gtagaaaagt tgctagactc caaagaacta acccgttttc
     1321 aaaaaattag cttgaagtat gcctttcaag agcatactcc aactcataaa tatgtgattt
     1381 cattaaataa acctgctaag ttaaccaatg ttcaaaaatt gatggagaaa tacaaacatg
     1441 gataaaatga aaccggtctt ccaagcccta aataaggaat taattcagga aaatctgact
     1501 ttaacaatta tctgtgtcga tggttatgtc ttagaatatc atggtttacg tgccacacaa
     1561 gatgttgatg ctttttatga ccaaaatcag aaaatcaatg aaattattgc tcgtgtagga
     1621 aaacagttta atcttaacat ccacgaagag ttatggctca acaatcatgt tgcaaagcaa
     1681 atatgaacaa acaacctccc ttaagtttat gcgaatcctt gtactcattt gagaatctga
     1741 ccgttttagt agtccctatt gagtatgtgt taggaatgaa gatgatgagt atcagagagc
     1801 aggatttgca agatattggt gcaattatta aatacaaaaa ctttcattca ccattcgata
     1861 cctttaaata tttaaaagac atgggatttg atactattga cctctctgtt ctattagaag
     1921 gttttagtta tgcttatggt atggattggt tagaaaaatt ttttaaagaa aatcaagata
     1981 aacttcgcga attttattaa cagtatcaaa caagatgatt gtcaattagc cagtcgtctt
     2041 gttttttatg atgaaatgaa ataaaatctg aacaaatcgc ttagagaatt ccagtcaatt
     2101 tctagcgctg tttgagacgt ttcagtggac tatagtaaaa tgaaataaga ataggacaaa
     2161 tcgatcagga cagtcaaatc gatttctaag aatgttttag aaataaaggt gtactattct
     2221 agtttcaatc tattatattt cagattcggt ccactattcc ttcccaaaaa cattgcatat
     2281 tgcgccgaag gaattgctca tactatccca agcacaaaac cctcagtcgt ttgaccgagg
     2341 gttccttttt attattcaag aacttgatta gctcaatgca tccaaggcgt catccattga
     2401 aagaacttcg tggaagacac gttgtgtcaa ttcagttttt tgttctggag tgaggtattt
     2461 agtgtttaca cagtatccag agatacgtac gataacgtct tcgcctgaca tgattttttc
     2521 gtaaacatcg ttcaagtcca taacgttcaa gttaacgtgt tgtccaccgt tttcgaagta
     2581 accatcaagg attgttacca agttatcaac ttgttcatca cgagtcttac caagagcgcg
     2641 aggtgatact tgtgtagtca atgagatacc gtcagctgca taactaaagt caaggctaga
     2701 aagtgagttc aagttttgca accaaccacc tttagctttg ttagatgggt tagcacctgg
     2761 tgagaagaat tcaagtttag acaagttcac agaaccatct tcgttgaggt atacaccttt
     2821 gtgaactggt gagttaccag tttgtttaga gtaagcaacg ttagatgtga ttgtcaaaag
     2881 tgatactgta gcttctgcgt ctttgtatag tttgtggcta cgtagacgag ttgtgtaagc
     2941 ttcgatcaac cattctgcca attcgtttga acgtgggtca tcttcacccc agcgtgggta
     3001 gtcaccgatt gtttcgtaat cgtagatgta gccatcttcg tcacggattg gtttaactgt
     3061 agcgtatttg atagctgaca atgtatcaac agtgttagca aatccacaga taccgaatcc
     3121 catgttggca cgttgtttag ttggcaagaa ggccatttga acagcttcgt agttgtacct
     3181 atcagtcatg tagtggatga tgttcaaggc atctacgtaa gtgtcagtca accagtcaag
     3241 agatttttca aagttcgctt taactgattc gaattcaaga acttcgtcac ggattggttc
     3301 gatatcaaat actttgtagt ctttgtgaac atcgtcgtaa ccaccattca aaccagtaag
     3361 aagggcttta agaacgttta cacgagcacc gaagtactgg atgttgtggc gttgttcttc
     3421 attttctgga tcaagtggag acacacagca tgagatacag ctcatttcac catatccgtc
     3481 tttagccatt gttgttacac cttcgtattg gatagaagag tgtttgtggc tcatgtgcat
     3541 acagtagcga cggaagttgt atggcaattt gtcagtccaa agaactgtca agtttggttc
     3601 tggtgagtta ccgatgttgt caagagtgtt caagaaacgg tagtccatct tagtaacacg
     3661 gtgacgaccg tcgttaccca taccagccat agaagttgtg ataaaggttg ggtcacctga
     3721 gtacaattgg tcataagctt ttgtacgagc aaatttaact gtacgaagtt tcataacgaa
     3781 atcatcaacg aattcttgga tttctgattc agtaaatgta ccacgagcaa ggtcacgttc
     3841 tgcaaagatg tccaatacga ttggtacacg acctagagat gtagcagcac cgttaatcac
     3901 acggcagaca gccatgaaag caatgttaac ccattggatt gcttctttca cgttcatcgc
     3961 tggtttgcga acatcaaccc cgtaaaggtc acccaggcga acaacttgtt gcaatgcttg
     4021 gtattgaagg tttacttctt cacgaagacg gattgtttct tcatcgattt ctttgattgc
     4081 attccagtcg tttacttttt cttgcatcaa gtagtctgca ccgtaaagag caagacgtgc
     4141 gtaaacaccg atgatacgtc cgcgtgagta tgcatctgga agaccagtta cagtgtgtgc
     4201 atgacgagcg cgacgaatat ttgaagtgta ggcacggaaa ataccgtcgt taactgttgt
     4261 tacatattta gtgaagattt cgtgaacagc tgggtctggt tcgtatccat tttcttttaa
     4321 agtagtttca gccatacgga taccaccttt tggcatgaag ttcaatttga agagttcatc
     4381 attttggata ccaaagataa cttcattttc tttgtcgata aatccagcag ggatatcagc
     4441 gatagatgtt ggacgagtgt ccattgggaa acgagtttct tcgtagtgtg ctttagtttc
     4501 ttctacaatt ttcttgatgt gaagtgaacg ctctgttggt cctgcaagga agctttcgtc
     4561 tccatcataa ggtgtgtagt tagcttgtac aaagcgtgat acacttgctt tttctttcca
     4621 atctacgcct ttgaagcctt cccaagcttt gtcaaaaata tcttgtgctt caacaactgt
     4681 cttaacaacc atattaatgt cctctttttt ctagtaacag tcatctgtta cattcataag
     4741 acaagtatac cataaagtaa ccgatttcaa caagtgcaaa atccctattt ttacactttc
     4801 ttttctaaaa cagtct
//
LOCUS       DAVKGC010000121         4682 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKGC010000121 DAVKGC010000000
VERSION     DAVKGC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.121"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(181..252)
                     /locus_tag="VW049_001837"
     tRNA            complement(181..252)
                     /locus_tag="VW049_001837"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(217..219),aa:Arg,seq:cct)
     gene            complement(295..642)
                     /gene="rplS"
                     /locus_tag="VW049_001838"
     CDS             complement(295..642)
                     /gene="rplS"
                     /locus_tag="VW049_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9432902.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(761..1090)
                     /gene="crcB"
                     /locus_tag="VW049_001839"
     CDS             complement(761..1090)
                     /gene="crcB"
                     /locus_tag="VW049_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9432903.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1084..1458)
                     /gene="crcB"
                     /locus_tag="VW049_001840"
     CDS             complement(1084..1458)
                     /gene="crcB"
                     /locus_tag="VW049_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9432904.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(1455..1721)
                     /locus_tag="VW049_001841"
     CDS             complement(1455..1721)
                     /locus_tag="VW049_001841"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9432905.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKCSRDYQDQNIK"
     gene            complement(1837..2280)
                     /locus_tag="VW049_001842"
     CDS             complement(1837..2280)
                     /locus_tag="VW049_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9432906.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            2384..3319
                     /locus_tag="VW049_001843"
     CDS             2384..3319
                     /locus_tag="VW049_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9432907.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3373..3657
                     /locus_tag="VW049_001844"
     CDS             3373..3657
                     /locus_tag="VW049_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9432908.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3714..3932)
                     /locus_tag="VW049_001845"
     CDS             complement(3714..3932)
                     /locus_tag="VW049_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432909.1"
                     /translation="MSISPRFETLEQAIAFKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(3929..4682)
                     /locus_tag="VW049_001846"
                     /pseudo
     CDS             complement(3929..4682)
                     /locus_tag="VW049_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1455 a         1028 c          842 g         1357 t
ORIGIN      
        1 acgattttga ataaattgac tatacaaaca tttatatact cgatatagtc tcaattttat
       61 ctgatgattg cccaaatttt tcaataataa aacgcataat attatggaga caatccccta
      121 tattatgcgt tcttttaata tcaaagactt tttgacaaac ttctttgata tctaattaca
      181 tgccccctgc aggaatcgaa cctgcaacta ctccttagga gggagttgtt atatccattg
      241 aactaaggga gctagataaa aactctgctg aaaaagcaga gttttttagt cgaattaacg
      301 acggatttct ttgatacgag ctgccttacc ttgaagagca cgcaagtagt acaatttcgc
      361 acgacgtact ttaccgtaac gaacaacttc gattttttca acacgtggag tgtggattgg
      421 gaagatacgc tcaacaccta caccgttaga gattttacga actgtgtagt tttctgagat
      481 tccagcacct ttacgtgcga taacaacacc ttcaaaaatc tggatacgtt cacggttacc
      541 ttcgacaact ttcgcatgta cacgaacagt gtcaccagga cggaatgatg ggatatctgt
      601 acgaagttga ccttcagtca agctttggat taatggattc attttattct cctatctttg
      661 tcaatcttga ggaaacttcc tcagcggata aactgtattt ttgtgcgtcc atcacacaca
      721 agatacagtc taccaaattt tcacaaaaaa gtaaagaaat ttatagcaaa attcctaaaa
      781 aaatcgcaac caaaccacct atgtaagtca aagtcaggta agaataaaag accttcttat
      841 cacttagcag tctttgaagt tcgtcattca aggtcgaaaa agttgttaaa ccgccacaaa
      901 atcctgttgc tagaatagca tatacttcct tagactctac atgattgtag aatactccaa
      961 ttaaaaaaca acctaaaaga ttagctatga gcgttccgag tggcaattta gaagcttgat
     1021 tgtagcggga aaagaaatac cgtacgagtg ctcccaagcc acaggcgatt gcaagataga
     1081 cgattaccat ttcttcctcc ctagaaaata agctaagagt aggcctccac cgatgctcaa
     1141 aagcaaatac agtatcaaac taagataacg ccctgtatca agcagcttca cagtatcaag
     1201 cattaggctg gaaaaagttg ttaaacctcc gcaaaaaccc gtccccagtg ctaaaatcaa
     1261 gcccttacta gtccccttat agaccagata gcccttgaca agataaatca agcagaaaat
     1321 tcccagatag ttgacaaaga gagtgcccca agggaaatct ggactggctg gtaaccaagt
     1381 ggaaactagg tagcggacaa gtccgcccaa catagcagct agaaaaatcc ctagcggata
     1441 aaattgttct tttttcattt gatattttga tcctgataat cacgcgaaca tttgagtatg
     1501 tccgaaaaag tcgcgacaat agtctcctga tagcgcttgt cctctacacg acttctaacc
     1561 ttttcaaaaa taactgcttc tctcttagta tctaaaatcg gtttccctga agccttctta
     1621 taagcgacaa ctccttcaac taagtgcatt cgttcttcta acagtttgac aatttggtcg
     1681 tcgatttgat cgatttcttg acgaataata tctaaatcca tacagtctcc tcctttattt
     1741 gaattattgt atcaaaaagc catcaattag gctagtaaaa tcccaactct cgataagaaa
     1801 aatgcactga ttgattacat cagcgcactt ttatgcttat cctactttag caaccaattc
     1861 ttctgcgaat tgttccaagc gttcaatatc ttcttcctcg gcagaaagat caactttaac
     1921 acactctgaa cctttttctg ctcctgttga cacaaagaca cggtcaaagt catcaacagc
     1981 cttacagaat tcatcgtaga aggtatctcc tgagccgacc actccgtaga ttttgccatt
     2041 caagttgaga tctgctaggt cttcgtagaa gtccatcatc tcatctggca attcaccatc
     2101 accataagta taggtcgcaa cgatagcgat gtctgcttcc aagaagtctg aagcgtcaac
     2161 agttgtacat tcatcaacat caacatccaa gcccaagtca cgtaatttgt ctgctacaat
     2221 atctgcaatt tcttcggtat taccggtcat actggcaaat acaatttttg ctaatgccat
     2281 atcgtcctcc tcaatttatc tttctccatt atatcacatc tttttcattt taaaaataaa
     2341 aattcttgtg ctacaatgaa atgagaaaga attgaggtta tttatggaga tttgccaaca
     2401 aattttagaa aaaattaaag aatacgacac cattatcatt catcgtcata tgaaaccaga
     2461 ccctgatgcc ttgggaagtc aggtgggatt gaaagccttg ctagaacatc atttcccaga
     2521 aaaaaccatc aaagccgtcg gttttgatga accaactctt acttggatgg ctgagatgga
     2581 tcttgttgaa gatagagcct accaaggcgc acttgtcatc gtctgtgata cagctaatac
     2641 tgctcgtatc gatgataagc gctatagtca aggtgatttt ctcattaaga ttgaccacca
     2701 tccaaatgat gatgtatacg gtgacctgtc ttgggtcgat actagttcaa gtagcgctag
     2761 cgagatgatt accctatttg cccaaacaac ccaactagcc ttggcagatc gcgatgctga
     2821 gttgctcttt gcaggaattg tcggtgatac aggtcgcttc ctctaccctt ctaccactgc
     2881 acggactctt cgcctggctg cttatttgag agaacataac tttgactttg cagctctcac
     2941 tcgcaaaatg gacactatga gctacaaaat tgctaaactg caaggctaca tctacgacca
     3001 tctggaagtg gatgaaaatg gtgctgctcg cgttatcctg agtcaagaag tcttgaaaca
     3061 atacaatgta accgatgctg aaactgcagc cattgtcggt gcacctggac gcattgacag
     3121 agtgagtctc tggggaattt ttgtcgaaca ggctgatggc cactaccgag ttcgcttacg
     3181 cagtaaaatc catcctatca atgaaatcgc caaggagcat gatggtggag gccaccctct
     3241 agcaagtggt gctaattcct atagcctaga agaaaatgaa atcatctacc aaaagttaaa
     3301 aaacttgctt aaaaactgat aaaatacttg ccaaactttt cagaatctga tagactagta
     3361 tggtaacaat ctatggctcg caaagagacc atggcagaaa ggaaatattg caaaatgaaa
     3421 aaagatatcc atccagaata tcgcccagtt gtcttcatgg acacaactac tggttaccaa
     3481 ttccttagcg gttcaacaaa acgctctaac gaaacagttg agttcgaagg cgaaacttac
     3541 ccattgatcc gtgtggaaat ttcatcagac tcacacccat tctacactgg acgtcaaaag
     3601 ttcactcaag cagatggacg cgtggatcgt ttcaacaaaa aatacggtct caaataatga
     3661 taaaagaaca gtttcgactg ttcttttttg tttcttgaaa tcaactgctg ttttcatgtt
     3721 ccagactcat ctgtaggttc ggtttccatg ctactaggca ggaaggaaat agctgtttca
     3781 acacgtccat aatgagctgt actattgtca cgaaccacac tttcattgat ggtccaagtg
     3841 gaattcattt tcttaaaagc ttctcggact ttttccaaat ctttgaaggc aatggcctgc
     3901 tctaaggttt caaaacgagg acttatactc atctgctttc acaaagcatt ctagtccatc
     3961 tccgattaac gatggacttt atcacctcct tctccagtcc ttgtatgaca tcttgaagtt
     4021 gattcatgat atcttccaaa gttcgaaagg ctttattctt aaatccacgt ttacgaatct
     4081 ctttccacac ttgttcaatg gggttcatct ctggtgtgta tggaggaata aaggtaaaac
     4141 caatattagt cggaatcttt aaggtacttg atttatgcca tatagcattg tccataacga
     4201 ataaaagata atcatctgga taagcttgtg aaagctcttc taaaaaggcg ttcatccact
     4261 cagtattaca tctaccagct attaagaaaa atgattcgcc tgtatgggca tcaacagctc
     4321 cataacaata gcgaaattct cgtatatagt gactatggac atgtggacct actcctattg
     4381 gagaccaaca agatcccagt ttactgattc taccgaaacc agcctcatct tggtacatca
     4441 agcgaacctt atgaaaacgt ctactatatt taaaacgctt tcttgtcttc ttgaattgag
     4501 attttatttt tagacgcgac aatggtttga gcgtctgctt tcttaggatg ttctggacgt
     4561 ggcataatat ttcgccaacc atggcgcttc aacagttgat agaaggcatc acgtgtgtag
     4621 gaacgatata agcctgaaat aaggtattgt aacaaattct cctgcctctg tagccttcaa
     4681 at
//
LOCUS       DAVKGC010000122         4578 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKGC010000122 DAVKGC010000000
VERSION     DAVKGC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.122"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(131..204)
                     /locus_tag="VW049_001847"
     tRNA            complement(131..204)
                     /locus_tag="VW049_001847"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Arg,seq:tct)
     gene            complement(248..727)
                     /gene="rlmH"
                     /locus_tag="VW049_001848"
     CDS             complement(248..727)
                     /gene="rlmH"
                     /locus_tag="VW049_001848"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9432910.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            910..2091
                     /locus_tag="VW049_001849"
     CDS             910..2091
                     /locus_tag="VW049_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9432911.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            2149..2907
                     /locus_tag="VW049_001850"
     CDS             2149..2907
                     /locus_tag="VW049_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9432912.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            3120..4481
                     /gene="dnaA"
                     /locus_tag="VW049_001851"
     CDS             3120..4481
                     /gene="dnaA"
                     /locus_tag="VW049_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9432913.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         1608 a          807 c          780 g         1383 t
ORIGIN      
        1 taacttacaa aaaccattat acaaaatgga atttcgtttt agataaaatt ctctcaactg
       61 tcattttttt ctcccaaagt gtactttttt aagaaaaaag ccgggaaaat tcccagcttt
      121 gctattatat tgatcccagc aggattcgaa cctgcgaccg ttcgcttaga aggcgaatgc
      181 tctatccagc tgagctatga gacctaatac aattattcta ccaaaaattc aattaaaagt
      241 caattttcta tttatggtag gggaatccct gctgaatcgt aaaagcgcga tagatttgtt
      301 caacaagaac tagtctcatt aactgatggg gtaaggttag gcgaccaaaa ctgacagaaa
      361 gattggctct attttttaca gatgatgaca atcctaaact tcccccaata ataaaagtaa
      421 gagtagaaaa tccttttata gaagtttctt ctaactgctt actaaattct tctgagaaga
      481 aagttttccc ttcaatggct aacacaataa cgaaatcacg gtcagcaatt tttgataaaa
      541 ttctctgacc ttctatttct aaaatctttt gattttctga ttcactggcc ttatctggtg
      601 ttttttcatc tgataactca atcatttcaa acttagcaaa tctagaaatt cgttttgaat
      661 actctgcgat accatctttt aaatactttt ctttcagttt cccaactgtt acaactttaa
      721 ttttcatgac tctattctaa catattctct attttttcac atcttattca caaaataaaa
      781 aatagatttc aattaagaaa atcacagttt caaaagagtt atccacagtt tgtgtaaaac
      841 ttttgtgttt aagttataat taagctagtc agtttatact ttcagtaatt caaacatatg
      901 gaggcaaata tgaaacatct aaaaacattt tacaaaaaat ggtttcaatt attagtcgtt
      961 atcgtcatta gcttttttag tggagccttg ggtagttttt caataactca actaactcaa
     1021 aaaagtagtg taaacaactc taacaacaat agtactatta cacaaactgc ctataagaac
     1081 gaaaattcaa caacacaggc tgttaacaaa gtaaaagatg ctgttgtttc tgttattact
     1141 tattcggcaa acagacaaaa tagcgtattt ggcaatgatg atactgacac agattctcag
     1201 cgaatctcta gtgaaggatc tggagttatt tataaaaaga atgataaaga agcttacatc
     1261 gtcaccaaca atcacgttat aaatggcgcc agcaaagtag atattcgatt gtcagatggg
     1321 actaaagtac ctggagaaat tgtcggagct gacactttct ctgatattgc tgtcgtaaaa
     1381 atctcttcag aaaaagtgac aacagtagct gagtttggtg attctagtaa gttaactgta
     1441 ggagaaactg ctattgccat cggtagcccg ttaggttctg aatatgcaaa tacagtcact
     1501 caaggtatcg tatccagtct caatagaaat gtatccttaa aatcggaaga tggacaagct
     1561 atttctacaa aagccatcca aactgatact gctattaacc caggtaactc tggcggccca
     1621 ctgatcaata ttcaagggca ggttatcgga attacctcaa gtaaaattgc tacaaatgga
     1681 ggaacatctg tagaaggtct tggtttcgca attcctgcaa atgatgctat caatattatt
     1741 gaacagttag aaaaaaacgg aaaagtgacg cgtccagctt tgggaatcca gatggttaat
     1801 ttatctaatg tgagtacaag cgacatcaga agactcaata ttccaagtaa tgttacatct
     1861 ggtgtagttg ttcgttcggt acaaagtaat atgcctgcca atggtcacct tgaaaaatac
     1921 gatgtaatta caaaagtaga tgacaaagag attgcttcat caacagactt acaaagtgct
     1981 ctttacaacc attctatcgg agacaccatt aagataacct actatcgtaa cgggaaagaa
     2041 gaaactacct ctatcaaact taacaagagt tctggtgatt tagaatctta attgacatct
     2101 atgtaaagaa agctttacat aagagaaaag atgtgttagt gtagaatcat ggaaaaattt
     2161 gaaatgattt ctatcacaga tatacaaaaa aatccctatc aaccccgaaa agaatttgat
     2221 agagaaaaac tagatgaact agcacagtct atcaaagaaa atggggtcat tcaaccgatt
     2281 attgttcgtc aatctcctgt tattggttat gaaatccttg caggagagag acgctatcgg
     2341 gcttcacttt tagctggtct acggtctatc ccagctgttg ttaaacagat ttcagaccaa
     2401 gagatgatgg tccagtccat tattgaaaat ttacagagag aaaatttaaa cccaatagaa
     2461 gaagcacgcg cctatgaatc tctcgtagag aaaggattca cccatgctga aattgcagat
     2521 aagatgggca agtctcgtcc atatatcagc aactccattc gtttactttc cttgccagaa
     2581 cagattcttt cagaagtaga aaatggcaaa ctatcacaag cccatgcgcg ttccctagtt
     2641 gggttaaata aggaacaaca agactatttc tttcaacgga ttatagaaga agatatttct
     2701 gtaaggaaat tagaagctct tctgacagag aaaaaacaaa agaaacagca aaaaactaat
     2761 catttcatac aaaatgaaga aaaacagtta agaaaactac tcggattaga tgtagaaatt
     2821 aaactatcta aaaaagacag tggaaaaatc attatttctt tttcaaatca agaagaatat
     2881 agtagaatta tcaacagcct gaaataaggc tgttctttta tttttttatc tcacaaggtt
     2941 atccactatg tttttcaata aaaagcttaa taaatcaata atttcttctt ttatccccaa
     3001 cctgtggata aagtttggta acattgtgga ttatttttca cagcttgtgg aaaattcttg
     3061 ctatctatgg taaaatatct ctagtattaa acttttaaat agtaaaggag gagaaaggat
     3121 tgaaagaaaa acaattttgg aatcgtatat tagaatttgc acaagaaaga ctgactcgat
     3181 ccatgtatga tttctatgct attcaagctg aactcatcaa ggtagaggaa aatgttgcca
     3241 ctatatttct acctcgctct gaaatggaaa tggtctggga aaaacaacta aaagatatta
     3301 ttgtagtagc tggttttgaa atttatgacg ctgaaataac tccccactat attttcacca
     3361 aacctcaaga tacgactagc tcacaagttg aagaagctac aaatttaact ctttataact
     3421 atagtccaaa gttagtatct attccttatt cagatacggg attaaaagaa aagtatacct
     3481 ttgataactt tattcaaggg gatggaaatg tttgggctgt gtcagccgct ttagctgtct
     3541 ctgaagattt ggctctgacc tataaccctc tttttatcta tggaggacca ggccttggta
     3601 agactcactt attaaacgct attggaaatg aaattctaaa aaatattcct aatgcgcgtg
     3661 ttaaatatat ccctgccgaa agctttatta atgactttct tgatcaccta agacttgggg
     3721 aaatggaaaa gtttaaaaag acctatcgta gtcttgatct tttgttaatc gatgatatcc
     3781 agtcactcag cggaaaaaaa gtcgcaactc aggaagaatt tttcaatacc tttaacgccc
     3841 ttcatgacaa gcaaaaacag attgtcctaa cgagtgatcg tagtccaaaa catctagaag
     3901 ggctcgagga gaggcttgtc acgcgtttta gttggggatt gacacaaact atcacacccc
     3961 ctgactttga aacacgtatt gccattttac aaagtaaaac ggaacattta ggctacaatt
     4021 tccaaagtga tactctagaa tacctagctg ggcaatttga ttcaaatgtt cgagatcttg
     4081 agggagccat caacgacatc actttaattg ccagagtaaa aaaaatcaag gatatcacta
     4141 ttgatattgc tgcagaagcc attagagccc gcaaacaaga tgttagccaa atgctcgtca
     4201 tcccaattga taaaatccaa actgaagttg gtaactttta tggtgttagt atcaaagaaa
     4261 tgaagggaag tagacgcctt caaaatattg ttttggcccg tcaagtagcc atgtatttat
     4321 ctagagaact aacagataat agtcttccaa aaattgggaa ggaatttggg ggaaaagatc
     4381 ataccacagt cattcatgcc catgccaaaa taaaatcttt gattgatcaa gacgataatt
     4441 tacgtttaga aattgaatca atcaaaaaga aaatcaaata atttgtggat aacttttagt
     4501 tttttatctt ttttatccac attttttaaa caagctaaaa aacttgatat gacttgttta
     4561 aaggctgttt tccacaga
//
LOCUS       DAVKGC010000123         4572 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKGC010000123 DAVKGC010000000
VERSION     DAVKGC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.123"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            173..1564
                     /locus_tag="VW049_001852"
     CDS             173..1564
                     /locus_tag="VW049_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEU9432914.1"
                     /translation="MRMLNQTIRVKSKYPYLIRPRNIKIVRLFIQIFVFLLLISSWIA
                     IIPFIARLVFSMFFISLLFFVEKFFRSNFIAKLRNKLNIREALFYMLISLNLYDELDN
                     EVVDTAVLYFDVENNKVVVCAPLFGNRYLKTLKNLEEYLCPTLGLSLLSKKEEIDKIV
                     YVLGQKEEIEQYVFNSNTLTREFFKDIPSSIIKLSNTQKFSLKSNTNLGIYGRTGTGK
                     TIALQWYLFNALAKGCGIADNTYLGIVDGKAADLYRIGELLHEELGEQVAVGSSPQML
                     AQLSRKFIENMDARFKIIKQNSSLNADIYELDELGLVPNFLFVDELASIRDSCGSTKQ
                     GRETWNEILQNLGLIARKGRQAGCHLVLSTQDPNAENIPVELRNQISAVLYLGAPGAD
                     RLKMAFSMCELENVPTVSDRKGEALFYADGLNTVEPVLTIVPFVDLKTKKEFLNVVRN
                     ILPTNDGLPPLAK"
     gene            1820..2239
                     /locus_tag="VW049_001853"
     CDS             1820..2239
                     /locus_tag="VW049_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432915.1"
                     /translation="MVYNMTDTNIKIGLLDLEMSQFDIPKKVVILSVRVNNKYKEVNG
                     ENVKTDEVSKITSAVLDADKVKVLTEMGISTDDLKAINLEIIGNLDKIAKLAQNDALL
                     NVPVELIRPKVRLAWNMARNNWSGVKIVCEDIKVLGE"
     gene            2244..3263
                     /locus_tag="VW049_001854"
     CDS             2244..3263
                     /locus_tag="VW049_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEU9432916.1"
                     /translation="MNTVRIDYFAVTVKNISPEKVLTDILLIPLENFTLNDWGINKYK
                     QHYSCSEIKVYFNADRLSMGVFIELKGQGCRQYEEFLNSNENNWIALISRLYHHNANF
                     TRLDIAHDIFDGSLDVQRIYDYCKKGLCISKAKHFEYHEKSVLDSGERVGETVAIGSR
                     GNQQWCIYNKRMEQLGKQELVEVSSWTRAELRCWQEKANIIAEQLYLKRPLSSIYFEA
                     INGHYRFIRPNAIDSNRWRRKKVEWWVNYLGTENQTVLSITRAKTTLRESEVWTEKQV
                     AKTLAKVYIAKYQAYDVQKAEDYIQNLLKEGLSKLTDNDEKDIEQYIREQNSSQLWGT
                     DGRSD"
     gene            3267..3449
                     /locus_tag="VW049_001855"
     CDS             3267..3449
                     /locus_tag="VW049_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEU9432917.1"
                     /translation="MADTISNKDLISMGYRPSTANAIIHQARELLVSRGYTFYNRKRL
                     MVVPKSVVKELLGMEL"
     gene            3451..3714
                     /locus_tag="VW049_001856"
     CDS             3451..3714
                     /locus_tag="VW049_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEU9432918.1"
                     /translation="MAYIEYKQRGKKRLWSFSIRERSKSLLHKSGFKTKREAKIEAEK
                     VLHKLNTGSVLSSSMTLSELYNEWLDLKILPSNRSVVTKKNIS"
     gene            3864..>4572
                     /locus_tag="VW049_001857"
     CDS             3864..>4572
                     /locus_tag="VW049_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9432919.1"
                     /translation="MAIADKVIIEDFTAYVELFSSKSGQKVEEKYLHAESDYQKVLVY
                     LKNKFDYQKSIVPYVIYFLFKTGMRFSELIALTWDEVDELNEQLKTYRRYNTAIHKFT
                     PPKNNTSIRLVPITSDMLSLLKTLKILQLKTNKELNIDNENNLIFQHFGYVYDVPDIA
                     TVNKAIKVMLKELQIFPLITTKGARHTYGSYLWHNNIDLGVIAKILGHKDISMLIDVY
                     GHTLEEKISEEFMAVKSL"
BASE COUNT         1535 a          671 c          960 g         1406 t
ORIGIN      
        1 tgtcttatac tgctaattat catgagcttc tttgcaggct tcggagctaa attagagttt
       61 acatcttata acccgttttt atctgttaaa tttggtttgg tcgtagctct gacagtcttt
      121 gtagttcaat tgttagccat actgccagcc atggttaaaa gaggaaataa aaatgagaat
      181 gttgaatcaa acaattcgtg tgaagagtaa atacccttac ttgattcgtc cccgaaatat
      241 taaaattgta agattgttta tccaaatttt tgtcttttta ctcttaatat cttcgtggat
      301 tgctataatc ccatttattg ctcgtctagt cttttccatg ttttttataa gtcttctttt
      361 tttcgtagag aagttctttc gttctaattt tatagcaaaa ttgagaaaca agctcaatat
      421 cagagaagcc ttgttttaca tgttaattag tttaaattta tatgatgagc tcgataatga
      481 agtagtagat acagcagttt tatattttga cgttgagaat aataaagttg ttgtctgtgc
      541 cccactattt gggaaccgtt atctgaaaac cttgaaaaat ttagaggaat atctctgtcc
      601 cactttgggc ttgtccttgc tgtctaaaaa agaagaaatc gataaaatag tatacgtgtt
      661 agggcagaaa gaagaaatag aacaatatgt ttttaatagt aacacattaa caagagaatt
      721 ttttaaagat attcctagtt caataatcaa gctgtcaaat acacagaaat ttagtctaaa
      781 gtccaacact aacctaggta tatatggaag aacggggaca ggcaaaacaa tagctctgca
      841 atggtactta tttaacgcac tggctaaagg ttgtggtatt gctgataata cttatttagg
      901 catagtagat ggtaaggcag ctgacttgta tcgtattggt gaacttcttc acgaagaact
      961 gggagaacaa gtggcagtag gctcttcacc acaaatgctt gcccaattat cgagaaaatt
     1021 cattgagaat atggatgcaa gatttaaaat tatcaaacag aatagttcat tgaatgctga
     1081 catttatgag ctagatgagc tgggactggt acctaatttt ttgttcgttg atgaactcgc
     1141 ttctattcgt gatagttgtg gctctacgaa acaaggtaga gaaacatgga atgaaattct
     1201 acaaaattta ggcttgattg cacgcaaagg acggcaggca ggttgccacc tagtcctatc
     1261 tactcaagac cctaatgccg aaaacatccc cgttgaattg aggaatcaaa tttcagcagt
     1321 tttatacctt ggtgccccag gggctgatag attgaagatg gcttttagca tgtgcgaatt
     1381 agaaaacgtt cctacagttt cagatagaaa aggggaggct ctcttttatg ctgatgggct
     1441 taatacagta gagccagtgc taacaattgt gccatttgtt gatttaaaaa ctaagaaaga
     1501 gtttttaaat gtagttagaa acatactgcc aactaatgac ggtcttcctc ccctagcgaa
     1561 atagggcgat tgccggaggc agaatcgagc ggagcgctct cttttcgcta ggggagggag
     1621 agccaaccga ctgctttgca ggaggtcaga aaaaacatcc ccagtactaa tagggggtgc
     1681 acacatacat gaaatgtact ctaaaccctt gatacgtgag gggttacggc ttactagttg
     1741 caccaagggg tcgcaaactc ttgggagagt agggatgaga ggtgtttcca tttatgatac
     1801 ggaattaaag gagaaaaata tggtttacaa tatgacagat acaaacatta aaattggatt
     1861 gcttgacctt gagatgtcac agtttgatat tcctaagaaa gtagtgattt tatcagttag
     1921 ggtgaacaat aaatacaaag aagtcaacgg cgaaaacgtg aagacagatg aagtaagtaa
     1981 aattacttct gctgttcttg acgctgacaa agtgaaagtt ctaactgaaa tgggaatttc
     2041 tactgatgac ttaaaagcta ttaacctaga aattatcgga aatctagata agattgcaaa
     2101 acttgctcaa aatgatgctt tgttgaatgt tcctgttgaa ttgattcggc ctaaagttcg
     2161 actagcttgg aacatggcta ggaataattg gtctggtgta aaaattgtgt gtgaggatat
     2221 taaagttcta ggagaataat cctatgaata cagttagaat agattatttt gctgtgacag
     2281 ttaagaatat ttcgccagaa aaagttctga cagacatcct tttgattcct cttgaaaact
     2341 ttaccttgaa tgattggggc ataaataagt ataagcaaca ttattcttgt tcagaaatta
     2401 aggtgtattt taatgctgat aggctttcta tgggtgtatt tattgaatta aaagggcaag
     2461 gttgtcgtca atatgaagaa tttttaaaca gtaacgaaaa taattggatt gctttgataa
     2521 gtcgtttata tcatcataat gccaacttta ctagactaga tattgcccac gacatatttg
     2581 acggtagctt agatgttcaa cgaatatatg actattgcaa gaaaggatta tgtatctcga
     2641 aagcaaaaca ctttgaatat catgagaaat cagttctaga ttctggtgag cgtgtaggag
     2701 aaacagttgc aataggttca cgaggtaatc agcagtggtg catatataat aaacgcatgg
     2761 agcaacttgg taagcaagag cttgtagaag tttcatcttg gactcgcgca gagcttagat
     2821 gttggcagga gaaggctaat ataattgcag agcagttata tttaaaacgt cccttatcgt
     2881 caatatattt tgaggctatc aacggtcatt atcgctttat tcgtccaaat gctatagata
     2941 gtaatcgttg gagaagaaaa aaggttgagt ggtgggtaaa ctatctaggg acagaaaatc
     3001 agacagttct gagtattaca agggctaaga cgacgttacg ggagtctgaa gtttggactg
     3061 agaaacaggt tgcaaaaaca ttagcaaaag tctatattgc aaagtatcag gcctatgatg
     3121 ttcaaaaggc ggaagattat attcaaaact tgctcaaaga gggactttca aagctaactg
     3181 ataatgatga aaaggatata gaacaatata taagagaaca gaatagttcc caactttggg
     3241 gaaccgatgg aaggagtgac tgacatatgg cagatactat atcaaataag gatttgattt
     3301 ccatgggcta taggccatca acagctaatg ctattatcca tcaggcgaga gaattacttg
     3361 tatcacgagg ctatacattt tataatcgca aacgtttgat ggttgttcca aaaagtgttg
     3421 tgaaagagtt gttgggaatg gaattgtgaa atggcttata tcgagtataa acagcgtggt
     3481 aagaaaagac tttggtcctt ctctatacga gaaaggagca agagcctact ccataaaagc
     3541 ggatttaaaa caaaacgaga agctaaaata gaagcggaga aagttcttca taagttaaat
     3601 actgggagtg tcttaagctc tagtatgact ttatcggagc tttataatga atggcttgat
     3661 ttaaaaattt tacctagcaa tagaagtgta gttactaaaa aaaatatctc atgagaaaga
     3721 aggtcatcga aaggttattt ggaaataagc ctgtatcaca aattaagcct agcgagtatc
     3781 aaaaaattat gaatgagtat ggagagactg tatcgagaaa tttcttagga agattgaatt
     3841 cttgtattcg ggcaagtata cagatggcta ttgctgataa ggtgataata gaggatttta
     3901 ctgcttatgt tgagttgttc tcttctaaga gtggacaaaa ggttgaggaa aagtatttgc
     3961 atgctgagtc agattatcaa aaagttttag tatatttgaa aaataagttt gattatcaaa
     4021 aatctattgt accgtatgta atttatttcc tttttaaaac tggcatgcgt ttctccgaat
     4081 tgattgctct aacttgggat gaagttgacg agcttaatga gcaacttaaa acttatcgtc
     4141 gttacaacac cgctattcat aagtttactc cacctaaaaa caatacttca ataagattag
     4201 ttcctattac ttcagatatg ctttccttgt taaaaacatt gaaaatattg caattaaaaa
     4261 caaataaaga attaaatatt gataatgaaa ataatttgat ttttcagcat tttggctatg
     4321 tctacgatgt tccagatatt gcaacagtca ataaagctat aaaagtaatg ttgaaggaat
     4381 tgcaaatttt tccattgatt actaccaaag gagctagaca cacttatggt agttatttat
     4441 ggcataataa tattgatttg ggagttatag cgaaaatttt aggacataaa gatatctcca
     4501 tgttgattga cgtttatggg cataccttgg aggaaaaaat tagtgaggag tttatggctg
     4561 tgaaaagttt gg
//
LOCUS       DAVKGC010000124         4513 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKGC010000124 DAVKGC010000000
VERSION     DAVKGC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.124"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..656)
                     /locus_tag="VW049_001858"
     CDS             complement(<1..656)
                     /locus_tag="VW049_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9432920.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     "
     gene            complement(838..1800)
                     /gene="nrdF"
                     /locus_tag="VW049_001859"
     CDS             complement(838..1800)
                     /gene="nrdF"
                     /locus_tag="VW049_001859"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9432921.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(1988..4147)
                     /gene="nrdE"
                     /locus_tag="VW049_001860"
     CDS             complement(1988..4147)
                     /gene="nrdE"
                     /locus_tag="VW049_001860"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9432922.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(4228..4446)
                     /gene="nrdH"
                     /locus_tag="VW049_001861"
     CDS             complement(4228..4446)
                     /gene="nrdH"
                     /locus_tag="VW049_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9432923.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
BASE COUNT         1313 a          840 c          920 g         1440 t
ORIGIN      
        1 taaaaatcaa acttattgaa cttactatga tctgcgagta aatatttttt attagaatta
       61 tttaaagcga tgcgttgagc ctctccctct tcctcgctaa aagtagctag agctccgttt
      121 tgaataccat tacagctaac gaaagcttta gaaaattgga gattagagag attttgtagg
      181 gtcaatgtgc caacaaaagc acctgtaata tcgcgataat ttccacctat caaaatcaaa
      241 tctgttaatt ttcgttcgct taaaatcaga aaaacaggta gactgttggt tacgacgcgg
      301 atattgtcaa taggcaactc acgcgcaaaa aactctaatg ttgttcctgg tccaatgaaa
      361 atagtttctc tttcttctac tagactgcct gcaaaatggg ctatttcttg tttttctgcc
      421 gtttggaggg cttgtttttc aatatttgat cgctcattag tcaaaaggga gttggttcga
      481 agtttttcag ctccaccatg cacacgaatc agcaaatctt tatcagctaa ttcctgtaaa
      541 tagcgccttg cagtcatatc tgaaacggct atttcgtcca taatctgttt aactgttatg
      601 gttcctttac tatttactat ctctaaaatt ttggttaatt tttcttgttt gagcatatta
      661 tcacctcgtt tcctactact atcttaccat aaacaaactc atcattcaaa tacaaaaaca
      721 acaaaatgaa acaaaaacaa aaatatcgaa gtttgttttc aaaactttcg atatttttgt
      781 tgggttataa ctttgatgtt tctagtttac tttttgatga ttgagagtga tggagaatta
      841 gtctaaaccg tagttatagt catcgtcttg catggcttca acttcgccaa gaaggtaacc
      901 atttccgact tgagagaaga agtcatggtt ggaagttcct gttgaaatac cgttcataac
      961 gattgggttg acatcttcag ctgaatctgg gaaaagtgga tcttgtccca tgttcatgag
     1021 agctttattg gcattgtagc gaaggaaggt tttaacctct tcagtccaac caacaccgtc
     1081 ataaagactc tctgtgtagc cttcttcatt ttcataaaga gtatagagta ggtcgtacat
     1141 ccattctttg agtttttctt gctcttcttc aggcaattca ttgaaaccaa gttggaattt
     1201 gtaaccaatg taggttccgt gaacagactc gtcacgaata atcaatttaa tgatttctgc
     1261 aacgttggct agtttgttgt taccgagata gtagagggga gtgaagaaac cagagtagaa
     1321 gaggaaggtt tcgaggaaga cgctggcaac tttcttttca agtgggctgc cgtttaggta
     1381 gatttcgttg acaatctcag ccttcttttg taggtaagga ttggtattgg tccattcgaa
     1441 aatttcttca atctcagcct tagtattcaa ggtagaaaag attgatgagt aagatttagc
     1501 gtggacagat tccataaatt ggatgttatt gaaaacagct tcctcatgtg gtgtacggat
     1561 gtctgcgcga agggcttgaa ccccagtttc agattgcata gtgtcaagaa gggttaaacc
     1621 accaaaaact tttccgacca agtctttctc tttgttagat agctttctcc agtcatccaa
     1681 gtcgtttgat aagggaatac gtgtatcgag ccaaaattgc tccgtcagtt tttcccaagt
     1741 tgatttgtcg atgacatctt cgatggcatt ccagttaatg gctttgtagt aagtttccat
     1801 ttaaaatctc tttctgtgtt tagtattgcg aactcacaat tatttctact ttaccataat
     1861 tctataggag tatcgcacaa aaagtcggaa gcccgacttt taaaatgtta cataaattat
     1921 gttatgacat agtagatttg attttatcag tgctgcttag ggaaaaatag tgtttctatg
     1981 atagaaacta aatcacacag ctttcacatt ggttggcgcc gacttctcca ccgtcatctg
     2041 taaaggtacg gacgtagtag atagacttga ttcccttgtt aaaggcatag ttacgaagga
     2101 tggacaagtc acgtgtcgtt tgtttatttt ccctcttcca ttcgtaaagg ccttttggaa
     2161 tgtcactacg catgaagagg gtgagtgaaa gtccttgatc cacgtgttca gtcgcagcag
     2221 cgtaaacatc gatgacttta cgcatatcca tatcgtaggc agaagtgtag taaggaatgg
     2281 tttctgtaga caagccagca gcagggtagt agattttacc aattttcttc tcttggcgtt
     2341 cttcgatacg ttgcgtaatt gggtggatag aagcagaaac gtcgttgata tagctgatag
     2401 aaccatttgg cgctacagca aggcgatttt ggtggtaaag accatcttct tgaaccttgt
     2461 cgcgaagttc agcccaatca gcaacaccag ggataaagac atttttgaag agttctttaa
     2521 cacggtctga tgttggaaca aattcaccag ttacatactt gtcaaagtaa cttccgttag
     2581 catagtctga tttttcaaag ttgtggaagg taataccacg ttcacgcgca atattgtttg
     2641 actctaccaa ggtccagtag ttcataagca taaagtagat gcttgtaaat tcaacagact
     2701 caggtgaacc atattcaatg agttgttggg caaggtagct gtgcagtccc atggcaccga
     2761 gaccaaaggt gtgggcttgg ctatttccat ggtcaatcgt tggtacagct acgatatgtg
     2821 aactatctgt aacgaaagta agggcacgaa ccatagcacg gatagaacga ccaaaatcag
     2881 gtgaagtcat catgttaacc acgttggttg aacccaggtt acatgaaaca tctgttccca
     2941 tttgaaggaa ttcttgagca tcgttgatca agcttggttc ttgaacttga agaatctcag
     3001 aacacaagtt actcatgata atctttccat caacaggatt tgcacggtta gccgtatcga
     3061 tgttgactac ataaggatag ccagactctt gttgcaattt agagatttca gtttccaaat
     3121 cccgcgcctt gatttttgtc ttgcgaatat ttggatttgc gaccaattca tcgtattttt
     3181 cagtaatgtc gatgtaattg aatggcacac cgtattcttt ttctacagag taagggctga
     3241 agaggtacat ttcttcattt ttacgagcca attcgtagaa tttatcaggt actacaacac
     3301 caagtgatag agtcttgaca cgtacttttt catcagcgtt ttctttctta gttgaaagga
     3361 aagcgatgat atctgggtga aagacgttga ggtagacaac accggcacct tgacgttgcc
     3421 ccaattggtt ggagtaagag aagctgtctt caaaaagctt catgacagga acgacacctg
     3481 aagcagctcc ttcatagcct ttgataggtg caccagcttc acgaaggttg ctgagggtaa
     3541 ttcccacacc accaccaata cgtgaaagtt gaagagctga gttgatagaa cgtccgatag
     3601 agttcatatc atccgtcact tggattagga aacaagatac caactcccca cgacgagcac
     3661 gtccagcatt caagaaggaa ggagtagcag gttggtagcg ttggtggatg atttcattgg
     3721 caatatcgat tgcaacagct tcattcccat cagcgaaata aagggcgttg aagaagacac
     3781 ggtcttccat attttcaaga tagtattcac cgtcattagt ctttaaggca tattgattgt
     3841 aaaatttata agctgccatg aatgacttga attggaagtt ttggtctttg ataaattgag
     3901 ccaattcttc caagaactct ggacggtatt tctttataaa ggctgtttcg atgtagttgt
     3961 gttcaatgag gtaattgatt ttgtctttga ttgaatcaaa aaccatagtg tttggaacta
     4021 cattttcttt aaagaaagca tccaaggctt ccttatcttt atgaagcatg atttgtccat
     4081 taacaggacg gttaatttcg ttattaagac ggaagtaagt cacgtcttca agatgtttta
     4141 atcccataaa atttccctta tctaattaca aaagaaaggc ttctaagtta gccctaaaag
     4201 cagtttcttc tggatgatgt actaagatta tgctaattgt ttcagttttc ctggttggaa
     4261 acctgaaaag acttcagttg gtgtttggat aacaggagct gcgctaaaac cgagctcttt
     4321 aacttgatcg acgtactcag gttgctcatc aagattgatt tcacgataag agacattatt
     4381 actgtccaag aaacgcttgg tcattttaca ttggacacaa ttgtttttag aataaacggt
     4441 taccattgtg taactcctct tcaaaattta atactatctt agtatatcag aaaataaaat
     4501 tttgtcaatg att
//
LOCUS       DAVKGC010000125         4169 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKGC010000125 DAVKGC010000000
VERSION     DAVKGC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.125"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            70..258
                     /locus_tag="VW049_001862"
     CDS             70..258
                     /locus_tag="VW049_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9432924.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            320..1078
                     /locus_tag="VW049_001863"
     CDS             320..1078
                     /locus_tag="VW049_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9432925.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFYEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYVGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            1136..1990
                     /locus_tag="VW049_001864"
     CDS             1136..1990
                     /locus_tag="VW049_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9432926.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            2102..2986
                     /locus_tag="VW049_001865"
     CDS             2102..2986
                     /locus_tag="VW049_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9432927.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKTFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            3148..3852
                     /locus_tag="VW049_001866"
     CDS             3148..3852
                     /locus_tag="VW049_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEU9432928.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSDTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDSVAWVRQGIF"
     gene            3828..3986
                     /locus_tag="VW049_001867"
     CDS             3828..3986
                     /locus_tag="VW049_001867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432929.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAK"
BASE COUNT         1401 a          602 c          881 g         1285 t
ORIGIN      
        1 ctaatgtatt ataaaagcca gttaacacaa gtgttacacc tgtgatagaa ttaacttgag
       61 gtgatgaaaa tggcaaccga taaaaataga ataatgatta gcttggatga taaaaatcta
      121 gagaaacttg aaaatttagt agaagatgct agagatagaa ggggaatgcg actaacaaag
      181 tcccaggtta ttgaattact cctaaatacc gttgattatt tcgatgatat tatgggggct
      241 atttactcaa aaaaataaga gcaaaattgc tattattttt tacaaattgg tgtaacacta
      301 gtgatacagg agtgatgaaa tggttgttta tattagacaa agtaaattac caagtgaggt
      361 ttcaattaat aaatacaatg ctcaagtagg tgcctactta caaggagaag aagttatttt
      421 atatcaatct ttttatgaaa taaaagagtt aacaagtgaa gatatagttg tagattatat
      481 aatggagact agagcattac taaaaatgat gggcttaaac gttccggttt atgactatcc
      541 tattgagctt aaagagtttt atggtcgaaa gatttatgtt ggtattttag gagagattgt
      601 gaatatacct gataattggg gaaaatttat taagcctaaa gctggttcaa aagtctttac
      661 tggaagagtt gttaatggaa cccatgattt aataggtatt ggtctacctt tcgactatcc
      721 tatatggatt agtgaggttg tagaattcat agctgaatgg cgttgttttg tgttagatgg
      781 tcgcgtatta gatgttcgac cctatacagg tgattatcat gcacaatttg atgcaagtgt
      841 aattgatgaa gcgatatcat gttggaaaga tgcgccaata gcttatggac tagatatcgg
      901 tgttactcgc gatggcagaa cacttgttgt tgaagtaaat gatggttatg cattgggaaa
      961 ttatggccta tctcctttaa aatcaatcaa ttttcataga gctaggtgga aagaaatggt
     1021 aaaaccctat tttgaaaaaa atgaaatttt taaaatccaa caggatgtta ttttctaaaa
     1081 taaatatggt agataaccaa tataaaaagc atcaaaatga acaaggagga atctgatgct
     1141 tggagcaatt attggagata ttgtcggttc tgtttacgaa tggaacaata tcaaaacaaa
     1201 ggactttcct ttatttcgga aggactgctt ttttacagat gacacggtta tgacttgtgc
     1261 tgttgcagaa gcaattatga atggtggaca aaaagacgac ttcattgacg cgatgaagaa
     1321 atatggcaga atgtatccga atgctgatta cggtgctcgg tttaatgcat ggctaaacag
     1381 cgataaccgt gagccttata atagctttgg taatggatca gctatgcgta tttctccatg
     1441 tgcttggatc atggactgtg gtttttatgc gaaaactggt atatggccat catctagagg
     1501 acttacgagt ctttctgcag aggtaactca taatcatcca gaaggtgtca aaggcgctat
     1561 ggctacagct gatgctatct ttctatgtcg tttttacttt ggtggttatt gtagagagta
     1621 cgagcaatca attaacgaca atcctacaga gtgtaaaaga cgaattaagg attatataga
     1681 aaaagaatac gattacaatc tatctcaaac tttagatgaa atccgtccta actatcgttt
     1741 taacgaaaca tgtcaggaaa ctgtccctca agccattatc gcctttcttg aaagtagaga
     1801 cttcgaagat gcgataagaa atgccatctc acttggtggc gacagtgata ctctcgcagc
     1861 aatcacttgt aacatagcag aggcagctta cggtattcct gattggatca aggataaggc
     1921 ctattcttac ttggatgaac ccttaaagga tgtagttagg cgatgggaaa atagaataaa
     1981 agcgtattaa tctccagtac gtcagatttc tcttttctgt attttcattg tataatagga
     2041 ttacatttac ttgcatacga cgaaatgtaa acatttctgt ttaaaattaa ggataaaatc
     2101 aatgaaaaaa gcaatggtaa ttatcaaccc tacttctggt ggcgagaagg ctttggatta
     2161 caaagaaaag ctggagaata aagcaaaaga atactttgaa tatgttgaaa ccaaaattac
     2221 cgaaaaagcg ctggatgcaa cacattttgc tgaagaagct tctcgtgagc agtatgatgc
     2281 agtggttgtg tttggtggag atggaactgt caatgaagtc atttcaggta ttgatgagag
     2341 agactacatt cctaagttag ggattatccc aggcggtacg ggtaacctca ttacaaaact
     2401 tttggaaatc aatcaagaca tcgatggcgc aattgacgaa ctggattttg atttaaccaa
     2461 taagattgat atcggtaaag caaatgacaa ctattttggt tatatcttta gtatcggttc
     2521 tctgcctgag gcgattcaca atgttgaaat agaggacaaa acaaaattcg gtattttaac
     2581 ctatgctgta aataccatga agtctgtcat gacagatcag gtctttaata ttaaggttga
     2641 gacagaaaat ggaaattatg ttggtgaagc tagccatgtt ttggtccttt tgacaaatta
     2701 cttcgctgat aagaaaacct ttgaagaaaa caaggacggc tatgccaaca ttttgattct
     2761 gaaagatgcc tctatattct ccaaattatc cgtcattcct gatttattaa aaggggatgt
     2821 tgtcgcaaat gataatatcg agtatatcaa agcgcgtaat attaaaatct cttcagatag
     2881 tgaattggag tcagatgttg acggagataa atcagataac ctacctgtag aaatcaaagt
     2941 cctagctcag cgagtagaag tattttcaaa accgaaagag gattagtata tagagaaagc
     3001 ctttttaagg ctttttgtat actttaaaag atagttcctt taacaacgga cattccttgt
     3061 aaatagttta caaaaatagt atactggatt tattgagttt gaaaacgttt gcgtaaaatt
     3121 tgaatgaata ctttaggaga caaattgatg gaattgagtg ctatttacca taggcctgag
     3181 tcggagtatg cctatcttta taaggataag aaactccata ttcgaattcg aactaagaaa
     3241 ggggacattg aaagcattaa cttgcactat ggggaccctt ttatctttat ggaggagttt
     3301 tatcaggata caaaagaaat ggtcaagata acttctggta ccttatttga ccattggcag
     3361 gttgaagtgt cagttgactt tgcacgtatc cagtatctct ttgagctcag agatacagaa
     3421 ggtcaaaata ttttgtatgg cgataaaggg tgtgtggaaa attctctaga aaatcttcat
     3481 gcaattggga atggatttaa gttgccttat cttcatgaga ttgatgcctg caaggttcct
     3541 gactgggttt cagatacggt atggtatcag atatttcctg aaagatttgc caatggcaat
     3601 gctctattaa acccagaagg gactttagac tgggattcat ctgtcacacc taagagcgat
     3661 gatttctttg gtggtgattt acaggggatt attgatcata tggattactt gcaagacttg
     3721 ggtattactg gactatatct ttgtcccatc tttgaatcta caagcaatca caagtacaat
     3781 acgacagatt actttgaaat tgaccgtcat tttggagaca gtgttgcatg ggtgcggcaa
     3841 gggatttttt agaactaatt aatcacaaag ggctacctct ttttaatccg cttgtaggtg
     3901 attctcatgt aaataataga caagaatatg acaatacagg gagtggaaat ttacaacctg
     3961 aaaagtggaa tgaaactgca aagtagcttt ataatgctat aatgtggttg attattttat
     4021 ggaatgctaa gccggataca cctttattta attttaaaga cgaagtaatt aagtataaaa
     4081 catatgagcc ttttgaaagc agtataaaaa gagtaaatac tactataaag aatggtagta
     4141 aagggaaaac tctgactgag atgattaat
//
LOCUS       DAVKGC010000126         4129 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKGC010000126 DAVKGC010000000
VERSION     DAVKGC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.126"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            40..>4129
                     /locus_tag="VW049_001868"
     CDS             40..>4129
                     /locus_tag="VW049_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9432930.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     AENKEKHEDIHSAMETSQDTEEKKTTIIEEKEVVSKNPVIDTKTSNEEAKIKEENSNK
                     SQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKELSSLKNTKVLYTYDRIF
                     NGGAIETTQDNLNKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFG
                     KNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAF
                     NYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSY
                     KMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAG
                     IPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFD
                     KVNIGGQSFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKI
                     AVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQV
                     FSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKE
                     IDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKST
                     YGYMSGTSMATPIVAASTVLIRPKLKEMLEKPVLKNLKGDDKIDLTSLTKIALQNTAR
                     PMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLK
                     EIKGDKKYFTIKLHNTSNRPLTFKVSASAVTTDSLTDRLKLDETYKDEKSPDGKQIVP
                     EIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEME
                     ALSSNGKKTDFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSKSKTMEGYDDDGKPKIP
                     GTLNKGIGGEHGIDKFNPAGVIQNRKDKNRTSLDQDPDLFAFNNQGVHAESTSGSKIA
                     NIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQKDLKVVSREHF
                     IRGILNSKGNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQ
                     FEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTY
                     HKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQ
                     GRNRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFANQADEKGMISY
                     YLVDPDQDSSKYQKLGEIPESKFKNLKNVKDDSLNKETAEVEN"
BASE COUNT         1603 a          618 c          824 g         1084 t
ORIGIN      
        1 ttatctattg gaagttatat aaatatgtaa ggaattaaaa tgaaaaaaag tacagtattg
       61 tcattaacca cagctgcagt tattttagca gcctatgccc ctaatgaggt agtcttagca
      121 gacacatcta gctctgaaga tgctttaagc atctctgata aagaaaaagt agcagaaaat
      181 aaagagaaac atgaagatat ccatagtgct atggaaactt cacaggatac tgaagaaaag
      241 aaaacaacaa ttattgagga aaaagaagtt gttagtaaaa atcctgtgat agacactaaa
      301 actagcaatg aagaagcaaa aatcaaagaa gaaaattcca ataaatccca aggagatcat
      361 acggactcat ttgtgaataa aaacacagaa aatcccaaaa aagaagataa agttgtctat
      421 attgctgaat ttaaagataa agaatctgga gaaaaagcaa tcaaggagct atccagtctt
      481 aagaatacaa aagttttata tacttatgat agaattttta acggtggtgc tattgaaaca
      541 actcaagata atctgaataa aattaaacaa atagaaggta ttacatcggt tgaaagggca
      601 caaaaagtcc aacccatgat gaatcatgcc agaaaggaaa ttggagttga ggaagctatt
      661 gattacctaa agtctatcaa tgctccgttt gggaaaaatt ttgatggtag aggtatggtc
      721 atttcaaata tcgatactgg aacagattat aggcataagg ctatgagaat cgatgatgat
      781 gccaaagcct caatgagatt taaaaaagaa gacttaaaag gaactgataa aaattattgg
      841 ttgagtgata aaatccctca tgcgttcaat tattataatg gtggcaaaat cactgtagaa
      901 aaatatgatg atggaaggga ttattttgac ccacatggga tgcatattgc agggattctt
      961 gctggaaatg atactgaaca agatatcaaa aactttaacg gcatagatgg aattgcgcct
     1021 aatgcacaaa ttttctctta taaaatgtat tctgacgcag gatctgggtt tgcgggtgat
     1081 gaaacaatgt ttcatgctat tgaagattct atcaaacaca acgttgatgt tgtttcggta
     1141 tcatctggtt ttacaggaac aggtcttgta ggtgagaaat attggcaagc tattagggca
     1201 ttaagaaaag caggcattcc aatggttgtc gctacgggta actatgcgac ttctgcttca
     1261 agttcttcat gggatttagt agcaaataat catctgaaaa tgaccgacac tggaaatgta
     1321 acacgaactg cagcacatga agatgcgata gcggtcgctt ctgctaaaaa tcaaacagtt
     1381 gagtttgata aggttaacat tggaggacaa agttttaaat acagaaatat aggggccttt
     1441 ttcgataaga ataaaatcac aacaaatgaa gatggaacaa aagctcctag taaattaaaa
     1501 tttgtatata taggcaaggg gcaagaccaa gatttgatag gtttggatct taggggcaaa
     1561 attgcagtaa tggatagaat ttatacaaag gatttaaaaa atgcttttaa aaaagctatg
     1621 gataagggtg cacgcgccat tatggttgta aatactgtaa attactacaa tagagataat
     1681 tggacagagc ttccagctat gggatatgaa gcggatgaag gtactaaaag tcaagtgttt
     1741 tcaatttcag gagatgatgg tgtaaagcta tggaacatga ttaatcctga taaaaaaact
     1801 gaagtcaaaa gaaataataa agaagatttt aaagataaat tggagcaata ctatccaatt
     1861 gatatggaaa gttttaattc caacaaaccg aatgtaggtg acgaaaaaga gattgacttt
     1921 aagtttgcac ctgacacaga caaagaactc tataaagaag atatcatcgt tccagcagga
     1981 tccacatctt gggggccaag aatagattta cttttaaaac ccgatgtttc agcacctggt
     2041 aaaaatatta aatccacgct taatgttatt aatggcaaat caacttatgg ctatatgtcc
     2101 ggaactagta tggcgactcc aatcgtagca gcttctactg ttttgattcg accaaaattg
     2161 aaggaaatgc ttgaaaaacc cgtattaaaa aatcttaaag gtgatgataa aatagacctt
     2221 acaagtctta caaaaatagc cctacaaaat actgcaagac ctatgatgga tgcaacttct
     2281 tggaaagaaa aaagtcaata ctttgcatca cctagacagc agggggcggg gctaattaat
     2341 gtggccaacg ctttgagaaa tgaagttgta gcgactttca aaaacacgga ttctaaaggt
     2401 ttggtaaatt catatggttc catttctctt aaagaaataa aaggtgataa aaaatacttt
     2461 acaattaagc ttcacaatac atccaacagg cctttaacct ttaaagtttc agcatcagcg
     2521 gtgactacag attctctaac tgacagacta aaactggatg aaacatataa agatgaaaaa
     2581 tctccagatg gtaagcaaat tgttccagaa attcatccag aaaaagtcaa aggggcaaat
     2641 attacatttg agcatgacac tttcactata ggggcaaatt ctagctttga tttaaatgcg
     2701 gttataaatg ttggggaggc taaaaataaa aataaatttg tagaatcatt tattcatttt
     2761 gagtcagtag aagaaatgga agcattaagc tccaatggca agaaaaccga cttccaacca
     2821 tctttatcaa tgcccctaat gggatttgct gggaattgga accacgaacc aatccttgat
     2881 aaatgggctt gggaagaagg atcaaaatca aaaacaatgg aaggttatga tgatgacggt
     2941 aaaccaaaaa ttccaggaac cttaaataag gggattggtg gagaacatgg tatagataaa
     3001 tttaatccag caggagttat ccaaaataga aaagataaaa atagaacatc tctagatcaa
     3061 gatccagact tatttgcctt taacaaccaa ggtgttcatg cagaatccac tagtggttct
     3121 aagattgcta acatttatcc tttagattca aatggaaatc ctcaagatgc tcaactagaa
     3181 agaggactaa ctccatctcc acttgttcta agaagtgcag aagaaggact aatttcaatt
     3241 gtaaacacca acaaagaagg agaaaaccaa aaagacctta aagttgtatc aagagagcac
     3301 ttcataagag gaattctaaa ttctaaggga aatgacgcta agggtatcaa atcatccaaa
     3361 ctaaaagttt ggggtgactt gaaatgggat ggactcatct ataacccaag aggtagagaa
     3421 gaaaatgccc cagaaagtaa ggataaccaa gatccagcta ctaagatacg aggacaattt
     3481 gaaccgattg cggaaggtca atatttctat aaatttaaat atagattaac taaagattac
     3541 ccatggcagg tttcctatat tcctgtaaaa attgataaca cagcccctaa gattgtttcg
     3601 gttgattttt caaatcctga aaaaattaag ctaatcacaa aggatactta tcacaaggta
     3661 aaagatcaat acaagaatga aactttattt gctagagatc aaaaagaaca tcctgaaaaa
     3721 tttgatgaga ttgcaaatga ggtgtggtat gcaggagctg ctcttgttaa tgaagatgga
     3781 gaggttgaga aaaatcttga agtaacttat gcaggtgagg gtcaaggaag aaataggaag
     3841 cttgataaag atgggaatac tatctatgaa attagcggag caggagatct aagaggaaaa
     3901 atcatcgaag taatcgccct agatggagca agcaacttta ccaaaatcca taggattaaa
     3961 tttgctaatc aagctgatga aaaagggatg atttcctatt atctagtaga tcctgatcag
     4021 gattcatcta aataccaaaa gcttggcgaa attcctgaat ctaaatttaa gaatttaaaa
     4081 aatgttaaag atgatagcct aaacaaagag acagctgagg tggaaaata
//
LOCUS       DAVKGC010000127         4091 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKGC010000127 DAVKGC010000000
VERSION     DAVKGC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.127"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            640..1632
                     /locus_tag="VW049_001869"
     CDS             640..1632
                     /locus_tag="VW049_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9432931.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1634..2452
                     /locus_tag="VW049_001870"
     CDS             1634..2452
                     /locus_tag="VW049_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9432932.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2454..3239
                     /locus_tag="VW049_001871"
     CDS             2454..3239
                     /locus_tag="VW049_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9432933.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3278..3556
                     /locus_tag="VW049_001872"
     CDS             3278..3556
                     /locus_tag="VW049_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9432934.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTGFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3541..3738
                     /locus_tag="VW049_001873"
     CDS             3541..3738
                     /locus_tag="VW049_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9432935.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFLQLWNPQNPCQILIKKLE"
BASE COUNT         1139 a          692 c          883 g         1377 t
ORIGIN      
        1 ctttttatat cactattgaa aaatttatac gagatgatga aagccttaag tgttatttta
       61 taaaggttat ttcaagtcgt tccaaggtaa caagtctaga tcagattgaa gctgataaaa
      121 cgatacaaag aaaatattca agtgagctaa aaaaatttat tggattttat aatgagatta
      181 tttgtgagga aaatagtttc ctacatgtac gaaagaggtg gtcgagttgg tttaggtagt
      241 cgatgcgtga gttgataatt ctcagggtat ggacttcttt ttcatgaatg aggtaaaaga
      301 gcaggtattg tttagagaca atcattctga gcatattttc tggatagagg gagtatccga
      361 ttttatgatc aaagttaata ccgccctctg gtgagaagat aagtaggttg gtaatttaaa
      421 ctattaaaca gaatttttga ttaaaagtat tattttatga gagaaatcct aatttcacaa
      481 tccataggca aacgcttgca tttcgttttt tattggacta taataggttg gtataaagcc
      541 ttttgtagta ataaaatgta gaaggtgtag aaagcaagga tttagaatat ttgtagttaa
      601 aaacacaatg ttgctattcc ttacgatagg gagatagata tggcaatgat agaagtggaa
      661 catcttcaga aaaattttgt gaagactgtt aaggaaccgg gcttgaaggg ggctttgcgc
      721 tcctttattc atcctgaaaa gcagaccttt gaagcggtca aggatttgac ctttgaggtt
      781 ccaaaagggc agattttagg atttatcggg gcaaatggtg ctgggaagtc gacaaccatt
      841 aaaatgctga caggaatttt gaaaccaaca tctggttttt gtcggattaa cggcaagatt
      901 ccccaggaca atcggcaaga ttatgtcaaa gatattggcg tagtctttgg acaacgcacc
      961 cagctatggt gggatttggc tctgcaagag acctacactg tcttaaaaga gatttatgat
     1021 gtgccaaact cgctctttca taagcgcatg gactttttga atgaagtctt ggatttgaag
     1081 gactttatca aggatcccgt gcggactctt tcactgggac aacggatgcg ggcggatatt
     1141 gcggcctcct tgctccacaa tcccaaggtt ctttttttag atgagccgac cattggtttg
     1201 gacgtttcgg ttaaggataa tattcgtcgg gcaattactc agatcaatca agaggaagaa
     1261 actaccattc ttttgaccac tcacgatttg agtgatattg agcaactttg tgatcggatt
     1321 ttcatgattg acaaggggca agagattttt gatggaacgg tgagccaact caaggagacc
     1381 tttggtaaga tgaagactct ctcttttgaa ctgctaccag gtcaaagtca tctcgtctct
     1441 cactatgaag gtctgtctga tatgaccatt gatagacaag gaaacagcct caacattgaa
     1501 tttgatagtt ctcgctacca gtcagctgac attatcaagc aaaccctgtc tgattttgaa
     1561 atccgcgatt tgaagatggt ggatacggat attgaggata ttatccgtcg cttctaccga
     1621 aaggagctct aggatgatca aattgtggag acgttataaa ccctttatca atgcaggggt
     1681 tcaggagttg attacttacc gagtcaactt tattctctat cggattggcg atgtcatggg
     1741 ggcttttgtg gccttttatc tctggaaggc tgtctttgat tcttcgcaag agtctttgat
     1801 tcagggcttc agtatggcgg atatcaccct ctacatcatc atgagttttg tgaccaatct
     1861 tctgactaga tccgattcgt cctttatgat tggggaggag gtcaaggatg gctccattat
     1921 catgcgtttg ttgcgaccag tgcattttgc ggcctcctat cttttcaccg agcttggttc
     1981 caagtggttg atttttatca gcgttggcct tccattttta agtgtcattg tcttgatgaa
     2041 aatcatatcg ggtcaaggta ttgtagaggt gctaggatta actgtccttt atctttttag
     2101 cttaacgctc gcctatctga ttaacttttt ctttaatatt tgctttggat tttcagcctt
     2161 tgtgtttaaa aatctttggg gttccaacct acttaagact tccatagtgg cttttatgtc
     2221 ggggagtttg attcccttgg cattctttcc aaaggttgtt tcagatattc tttccttttt
     2281 gcctttttca tccttgattt atactccagt tatgatcatt gttggaaaat acgatgccag
     2341 tcagattctt caggcactcc ttttgcagtt cttctggctc ttagtgatgg tgggattgtc
     2401 tcagttgatt tggaaacggg tccagtcctt tatcaccatt caaggaggtt agtatgaaaa
     2461 aatatcaacg aatgcatctg atttttatca gacaatacat caaacaaatc atggaatata
     2521 aggtggattt tgtggttggt gtcttgggag tctttctggc tcaaggcttg aatctcttgt
     2581 ttctcaatgt catctttcaa catattccat tcctagaagg ctggaccttt caagagatag
     2641 ctttcattta tggattttcc ttgattccca agggaatgga ccatctcttt tttgacaatc
     2701 tctgggcact agggcaacgc ctagtccgaa aaggagagtt tgacaagtat ctgactcgtc
     2761 ccatcaatcc tctctttcac atcctagttg aaacctttca gattgatgcc ttgggtgaac
     2821 tcttagtcgg tggtatttta ttgggaacaa cagtgaccag cattgtttgg actcttccaa
     2881 aattcctgct tttcctagtt tgtattcctt ttgcgacctt gatttatact tctcttaaaa
     2941 tcgcaacagc cagtatcgcc ttttggacta agcagtcagg cgccatgatt tacatcttct
     3001 atatgttcaa tgactttgct aagtatccga tttctattta caattctctt cttcgttggt
     3061 tgattagctt tatcgtgcct ttcgccttta cagcctatta tccagctagc tatttcttac
     3121 aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt gatttctctg gttttctttg
     3181 ttatttccct taaactttgg gataagggct tagattccta cgaaagtgcg ggttcgtaaa
     3241 agctaaagta agactaaaat caagaaagaa acttatgatg tttgtaattg aagaagtcaa
     3301 ggatgaaaat caaaaaaagg cagttgtagc tgaggttttg aaggatttgc cagaatggtt
     3361 tggaatccca gaaagcacac aagcctatat agaaggaacc acgacactgc aagtttggac
     3421 cgcctatcag gagagtgatt tgactggatt tgtaagctta tcctattcga gtgaagattg
     3481 tgcagagatt gattgtctcg gcgtaaaaaa gcttatcaag gtagaaaaat tgggagccaa
     3541 ttgcttgcta ctttagagag tgaagctcgt aaaaaagttg gttatctgca ggtcaaaaca
     3601 gtggcagaag gttctaataa agattatgat cgaacaaatg acttttatcg aggtcttggc
     3661 tttaaaaagt tagagatttt tcttcaacta tggaatccgc aaaatccttg tcagattttg
     3721 attaaaaagc ttgaataata ttacttgaca tctattctca gagtgctata ctgtaagtgt
     3781 aatcgccgat ttagcttagt tggtagagca aggcactcgt aaagcctagg ttataggtag
     3841 ataaacgact gaggatttga aaaaatagat aggtagaaga taaccgttaa gccttattct
     3901 tagcggttat ttatattgtt taatagcgct gatatttcat caattatgcc tgttttcgtg
     3961 tttctggtag ttgttcaagt ttattgctac tatttttgat ggtatgaatg tgcttataat
     4021 gtatccctgt taagtacgaa acttttgcat tattttttaa catatattaa aaaataaatt
     4081 aatgtgtatt a
//
LOCUS       DAVKGC010000128         3867 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKGC010000128 DAVKGC010000000
VERSION     DAVKGC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.128"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            7..1782
                     /locus_tag="VW049_001874"
     CDS             7..1782
                     /locus_tag="VW049_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEU9432936.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            1783..3168
                     /locus_tag="VW049_001875"
     CDS             1783..3168
                     /locus_tag="VW049_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEU9432937.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            3191..3802
                     /locus_tag="VW049_001876"
     CDS             3191..3802
                     /locus_tag="VW049_001876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEU9432938.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         1247 a          574 c          855 g         1191 t
ORIGIN      
        1 ataactttga ctcaagggaa gattataaaa gtatcgggac ctctagttat tgcatcaggt
       61 atgcaggagg ctaatattca agatatttgc cgtgtaggta agctagggtt aatcggtgaa
      121 attattgaaa tgagaagaga tcaggcatct atccaagtct atgaagaaac atctggtctt
      181 ggtccgggag aacctgttgt tacaactgga gaacctctct cggttgaatt agggccagga
      241 ttgatttctc aaatgtttga tggcatacaa cgcccattag atcgatttaa attggctact
      301 cataatgatt ttctagttcg tggggtagaa gttccaagtt tggatagaga tattaagtgg
      361 cattttgatt ccactatagc aattggtcaa aaagtgagta cgggtgatat tcttggaact
      421 gtcaaggaaa ccgaggtagt taatcataaa attatggttc cttatggagt atctggagaa
      481 gtcgtttcta ttgcatctgg cgattttaca attgatgaag ttgtatatga aataaaaaaa
      541 ttggacggta gtttctataa aggaacgctt atgcaaaaat ggcctgtccg caaggcgcgt
      601 cctgtttcta aacgtttaat tccagaagaa ccattaatca caggtcaacg agttattgat
      661 gcattctttc cagtaaccaa agggggagct gcagcagttc ctggaccgtt tggagcagga
      721 aagacagttg tacaacacca agtagctaaa tttgccaatg ttgatattgt tatttatgtc
      781 ggttgtggag aacgtggaaa tgaaatgacg gatgtactga atgagtttcc tgagttgatt
      841 gaccctaata ccggacaatc aattatgcaa cggacagttc tgattgctaa tacttcaaat
      901 atgcctgttg ctgctcgtga ggcttcaatt tatacaggaa ttaccatggc tgagtatttt
      961 cgtgatatgg gctactctgt cgccattatg gctgattcaa cttcacgttg ggcagaagcg
     1021 ctacgtgaaa tgtcaggacg tctagaagaa atgcctggtg atgagggtta tcctgcttat
     1081 ctgggaagtc gtatcgctga atattatgaa agagcaggac gttctcaggt tctagggctt
     1141 ccagaacgtg aaggaacgat tactgctatt ggagctgtat cgccacctgg tggagatatt
     1201 tcagaaccag ttactcaaaa cactttacgg attgtgaaag ttttttgggg gcttgatgct
     1261 ccgttggcac agcgacgtca ttttcctgca attaactggc ttacatctta ttcactatat
     1321 aaagacagtg tgggcactta tatagatggt aaagagaaga cagattggaa tagtaaaata
     1381 actcgtgcga tgaactactt acaacgggaa tctagtttag aggaaattgt tcgtcttgtt
     1441 ggaattgatt ctctgtctga taatgaacga ctaacgatgg aaattgctaa acaaattcga
     1501 gaagattatt tgcaacagaa cgcttttgat tcggtagata cattcacttc gtttgcaaaa
     1561 caagaagcaa tgctaagtaa tattctcact tttgctgatc aggcaaatca tgctttagag
     1621 ttgggttctt actttacaga gattatggaa ggtaccgtgg cagttcgaga ccgtatggcg
     1681 agaagtaaat atgtttcaga agatagatta gatgaaatca aaattatatc aaatgagatt
     1741 acacatcaaa ttcatttgat attagaaaca ggaggtctat aaatgagtgt tataaaagaa
     1801 tacagaactg ctagtgaagt tgttgggcct cttatgattg ttgaacaagt aaataatgtg
     1861 tcttacaatg agttagttga aattcaactt cataatggag aaattcgtcg tggacaagtt
     1921 ttagagatcc acgaagataa agcaatggtt cagctttttg aaggatctag tggaataaat
     1981 ttagaaaagt ctaaaattcg ttttgctggt catgcattag aattggctgt atctgaggat
     2041 atggttggtc gtatttttaa tgggatggga aaaccaattg atggtggacc agatttaatt
     2101 ccagagaaat atttagatat tgatggtcaa gctattaatc ctgtatctag agattatcca
     2161 gatgaattta ttcagacagg gatctcctct attgatcatt tgaatactct tgtacgtggt
     2221 caaaaattac cagtattttc aggttcgggc ttacctcata atgaattagc tgctcagata
     2281 gcaagacaag cgactgtttt aaattctgat gaaaattttg cggttgtatt tgcagcaatg
     2341 ggtattactt ttgaagaagc tgagtttttt atggaagaac tcagaaaaac aggagcgatc
     2401 gatcgttcgg ttttatttat gaacttggca aatgatcctg caattgagcg tattgcaact
     2461 ccccgcattg ctttaactgc ggcagagtat ctagcttttg aaaaagatat gcacgttcta
     2521 gttatcatga cggatatgac taactattgt gaagcgttac gtgaagtctc ggcagctcgc
     2581 cgtgaagttc cagggagacg aggctatccg ggatatttat atacaaattt atcaactcta
     2641 tacgaaaggg ctggtcgctt agttggtaaa aaaggttcgg tgacacagat tcctatttta
     2701 acaatgccag aagatgacat aacacatcca attcctgatt taactggata cattactgaa
     2761 gggcaaatta ttttgtcgca tgagttgtat aatcaaggtt atcgtccacc aatcaatgtt
     2821 ttaccttctc tctctcgatt aaaagataag ggatctggag aaggtaaaac tcgtggagat
     2881 catgctccaa ctatgaatca actgtttgca gcctatgccc aagggaaaaa ggttgaagag
     2941 ttagcagtag tattaggaga atcggcttta tctgatgtag ataaattgta tgtgaggttt
     3001 acaaagcgtt ttgaagaaga gtacataaac caaggatttt ataaaaatcg aaatatagaa
     3061 gatacgttga atcttgggtg ggaattacta tcaattcttc ctagaacaga gttaaaacgt
     3121 atcaaagatg atttgcttga taaatactta cctttggtag aagtttaatc cggaaatgga
     3181 gtgattatct atggtacgtt tgaatgtaaa accaactcgt atggaattga ataacttaaa
     3241 ggaacgtttg acaacagctg aacgtggaca taagttatta aaggataaaa gagatgaatt
     3301 gatgaggcga tttatttctt tgattcgtga gaataatcaa cttcggaaag aagtggaaag
     3361 ttatctaatt gataatctaa aatcctttgc agttgctaaa tcattaaaga attctcaaat
     3421 ggtggaggaa ttattttcaa ttccatcgaa agaaattgaa ttatttgttg agaaagaaaa
     3481 tatcatgagt gtaacagttc ctagaatgca tatgaatatt acttctcaaa atgagaacag
     3541 tgaatacagc tatttatctt ctaatagtga aatggatgat gtatttgcta caatgaatag
     3601 tttaatttat aaattactaa gactggcaga agttgaaaaa acgtgtcagt taatggctga
     3661 tgaaatagaa aaaacacgta gacgtgtaaa tggtttagaa tactcgatta ttccaaactt
     3721 gtcggaaact attcattata tagaattgaa actagaggag gcagaaagag ccaatttagt
     3781 tcgtattatg aaagtgaagt agatccttta tttagattat taattagatg aacaaatatc
     3841 agcttggata aggctttaag cctttct
//
LOCUS       DAVKGC010000129         3807 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKGC010000129 DAVKGC010000000
VERSION     DAVKGC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.129"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            160..840
                     /locus_tag="VW049_001877"
     CDS             160..840
                     /locus_tag="VW049_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9432939.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLETISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRIRHAGFG
                     NPSTLSRK"
     gene            800..1477
                     /locus_tag="VW049_001878"
     CDS             800..1477
                     /locus_tag="VW049_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9432940.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSVIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTTSFWIYGTILILYFAVCYPISKLSTH
                     LEKHWRN"
     gene            1478..2236
                     /locus_tag="VW049_001879"
                     /pseudo
     CDS             1478..2236
                     /locus_tag="VW049_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            2248..3108
                     /locus_tag="VW049_001880"
     CDS             2248..3108
                     /locus_tag="VW049_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9432941.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAK
                     ADDLVVEGGH"
     gene            complement(3188..3802)
                     /locus_tag="VW049_001881"
     CDS             complement(3188..3802)
                     /locus_tag="VW049_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9432942.1"
                     /translation="MKLKKTCVFQNLNFSCKPGEIIGITGANGSGKSVLFKLIAGLYS
                     PSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLASIRGVVGNQEIN
                     DTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMNALDKSSVENMR
                     TLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
BASE COUNT         1104 a          838 c          697 g         1168 t
ORIGIN      
        1 ctcttttttc aaaacttttc aaatattttg tttggaaatt tcagaaattt tatgtctatg
       61 gtaaaaaatc cttataacgg caataaaaaa tagatattat ccaaagaaga ttttaagtgc
      121 tacaataact gtattatttc tagatgggag gttctatttt tggattggtc cattgttgaa
      181 caatatctac cactatatca aaaggcattc tttctgacct tgcatattgc agtttgggga
      241 attttgggat cctttctgct cggtttaatc gttagtatca tccgacatta tcgaatccct
      301 gttttggcgc aagtagcgac agcctacatt gaattgtcac gtaatacgcc ccttttgatt
      361 caactcttct ttctctactt cggtcttccc cgaatcggca ttgtcctatc ttcagaagtc
      421 tgtgcaacgc ttgggcttgt ctttttagga ggctcctata tggcagaatc tttccgaagt
      481 gggctggaaa ccatcagtca aacccagcag gagattggcc tcgctattgg tctgacacct
      541 ctacaggtct ttcgctatgt ggttcttccg caagcaacag cggtggcact cccctccttt
      601 agtgccaatg tcattttcct tatcaaggaa acctctgttt tctcagcagt ggctttggcc
      661 gacctcatgt acgtcgccaa ggatttgatt ggtctctact atgagacaga cattgcgcta
      721 gctatgttgg tagttgctta tctgatcatg ctgctaccca tctcactggt ctttagctgg
      781 atagaaagga ggatccgcca tgcaggattc gggaatccaa gtactctttc aaggaaataa
      841 cctcctgaga atcttacagg gattgggcgt tacgattggg atatccatcc tgtctgtcct
      901 cttatccatg atgttcggaa cagtcatggg aatcatcatg acctcccatt ctagaatcat
      961 acgattttta acacgattgt atctggaatt tatccgtatc atgccccagc tggtgctact
     1021 cttcatcgtt tactttggct tggctcgaaa ctttaatatc aatatctcag gtgagacttc
     1081 agttattatc gtttttaccc tctggggaac agctgaaatg ggagacttgg tacgtggagc
     1141 tatcacttct ctccctaaac atcagtttga aagtggacag gcactcggct tgactaatgt
     1201 tcaactttac taccacatca tcatcccaca agtcttgaga agactgctac cgcaggctat
     1261 caatcttgtc actcggatga ttaaaaccac ttcattagtt gttttgattg gggttgtgga
     1321 agtgaccaaa gttggacaac aaatcatcga tagcaatcgc ctgaccatcc caactacttc
     1381 attttggatt tatggaacca ttctaatctt atatttcgca gtttgctacc ctatttccaa
     1441 actatccact cacttagaaa aacattggag aaactaaatg tctgaaacta tcttagaaat
     1501 caaggaacta aaaaaatcct tcggagacaa tcccatcctc caaggacttt ctctagaaat
     1561 caaaaaaggg gaagttgttg tcatcctagg gccatctggt tgtgggaaaa gtaccctcct
     1621 tcgttgcctc aacggcttag aaagtattca aggtggagat attcttctgg atggtcagtc
     1681 tatcgttgaa aataaaaaag attttcacct agttcgccaa aagattggca tggtctttta
     1741 aagttatgaa ctctttcccc atctggatgt cttacaaaac ctcatcctag gccctatcaa
     1801 aactcaagga tgggacaaga aagaagtaac ggaagaagct ttgcaattac tagatcgtgt
     1861 cggtttgctg gataaacaac atagctttgc ccgtcaatta tctggtggac agaagcaacg
     1921 tgttgcaatt gtccgtgccc tcctaatgca tccagaaatc atcctttttg acgaggtgac
     1981 tgcttcgctg gatccagaaa tggtgcgtga ggtgctggaa cttatcaatg atttggccca
     2041 agaaggccgt accatgattt tagtaaccca cgaaatgcag tttgcccaag ccattgctga
     2101 ccggattatc ttcctcgacc aagggaaaat cgctgaagaa ggaacagctc aagccttctt
     2161 taccaatccg caaaccaaac gagcccagga atttttaaac gtctttgact ttagccaatt
     2221 cggctcatat ctataaagga gattcttatg aaactattca aaccactctt aactgtttta
     2281 gcacttgcct ttgcccttat ctttatcact gcttgtagct caggtggaaa cgctggttca
     2341 tcctctggaa aaacaactgc caaagctcgc actatcgatg aaatcaaaaa aagcggtgaa
     2401 ctgcgaatcg ccgtgtttgg agataaaaaa ccgtttggct acgttgacaa tgatggttct
     2461 taccaaggct acgatattga actagggaac caactagctc aagaccttgg tgtcaaggtt
     2521 aaatacattt cagtcgatgc tgccaaccgt gcggaatact tgatttcaaa caaggtagat
     2581 attactcttg ctaactttac agtaactgac gaacgtaaga aacaagttga ttttgccctt
     2641 ccatatatga aagtttctct gggtgtcgta tcacctaaga ctggtctcat tacagacgtc
     2701 aaacaacttg aaggtaaaac cttaattgtc acaaaaggaa cgactgctga gacttatttt
     2761 gaaaagaatc atccagaaat caaactccaa aaatacgacc aatacagtga ctcttaccaa
     2821 gctcttcttg acggacgtgg agatgccttt tcaactgaca atacggaagt tctagcttgg
     2881 gcgcttgaaa ataaaggatt tgaagtagga attacttccc tcggtgatcc cgataccatt
     2941 gcggcagcag ttcaaaaagg caaccaagaa ttgctagact tcatcaataa agatattgaa
     3001 aaattaggca aggaaaactt cttccacaag gcctatgaaa aaacacttca cccaacctac
     3061 ggtgacgctg ctaaagcaga tgacctggtt gttgaaggtg gccattaact cttaaaaggg
     3121 actggatttt aagctccaat ccctttttaa gattttacct ataacatcct gagtctatct
     3181 aagatgttca atctgaacac agtgtacata ctttatcttc tattgcatat actttatcac
     3241 ataagatacg aatatcctct tcactatgac tagcaatcaa aattgttgtc cctttttcac
     3301 tagagagctt tctaaacaat gttctcatat tttctacact tgatttatcc aaggcattca
     3361 taggttcatc tagtaaaaga atagagggat tctccataat tgcttgagca atccctagct
     3421 ttttcctcat acctagcgaa taagttttaa ctttctggtc tttttgctca tatagaccaa
     3481 ctattttcag tgtatcattg atttcctgat taccaactac tcctcgtatg cttgccaaat
     3541 attgtaaatt cttaaagcca ctataataat ttataaaacc aggttcttca atcaaagctc
     3601 ccaaattagc tggaattttt ctctcaggaa caatattttc cccattgatt aacacttctc
     3661 cataagacgg actatataaa ccagctatta atttaaacaa tacacttttc cctgagccat
     3721 tcgcaccagt gattcctatg atttcccctg gtttacaact aaagttaagg ttttgaaaaa
     3781 cacatgtctt ttttaatttc aactcaa
//
LOCUS       DAVKGC010000130         3725 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKGC010000130 DAVKGC010000000
VERSION     DAVKGC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.130"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..223)
                     /locus_tag="VW049_001882"
     CDS             complement(<1..223)
                     /locus_tag="VW049_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9432943.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELTEGICKQGQISRLENGEFTPG
                     ADFLYALSKKLKVSVDYFFNEQIVEEIDEL"
     gene            complement(291..2114)
                     /locus_tag="VW049_001883"
     CDS             complement(291..2114)
                     /locus_tag="VW049_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU9432944.1"
                     /translation="MVITKHFAVHGKCYRKKIIKYILNPEKTENLALVSHYGMRNFLD
                     FPSYEEMVQMYHENFISNDTLYDFRHDRHEENQRLIHAHHIIQSFSPEDHLTPKQINQ
                     IGYETMKELTGGKFRFIVATHVDKNHFHNHIIVNSVDNNSDKKLKWNYKVERNLRMIS
                     DRFSKIAGAKIIENRYSYHQYKVYRKTNHKYELKQRLYFLMENSTDFEDFKKNAPLLH
                     VKMDFSHKHATFFMTDSTMRQVVRGNKLNHKQAYTEEFFKNYFAKRKMEELMEFILPK
                     VDNLEDLIKKAKLFGLNINPKQKHVSFQFAGVEVKETELDKKNLYDVEFFKDYFEKRK
                     DLKAPEVQDLVQVYQEERFSKEKELPSEEKFWESYQEFRRNRDAIHEFEVELSLNQIE
                     KVVDDGIYIKVKFGIRQEGLIFLPNMELDMEEDKVKVFIRETSSYYVYYKDDAEKNCY
                     MKGRTLIRQFSSENQTIPFRRKTTVEMIKEKIEEVYTLLELDTDKHSYITIKDELVHE
                     LAASELRINAFQERVTTLNQVAEYLLASVENKQEMKMNLSKLNITDKIGIDVVEKELN
                     ELGIQIELESDRYENIVFKLNKFINRLSKKISKEEGIGFQK"
     gene            complement(2101..2466)
                     /locus_tag="VW049_001884"
     CDS             complement(2101..2466)
                     /locus_tag="VW049_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU9432945.1"
                     /translation="MENRYRTNLKKVFLSDSELGQLKHCIDQSGCQSFSEYARRTLLD
                     PGMNFITIDTTGYQDLVFELKRIGNNINQIARSVNQSQLISSEELQDLKKGIADLIRE
                     VEKEFSIQAKKLREFHGHH"
     gene            complement(2471..2827)
                     /locus_tag="VW049_001885"
     CDS             complement(2471..2827)
                     /locus_tag="VW049_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432946.1"
                     /translation="MPEQYRDIRKEVNLTANELEMIGVMMKNKGFEHFSSFARNKLLE
                     NELEMTAEKWFTFWRAQKLKQISRDVYEILILSRAEHQVTQEHVSILLTCVQELIQEI
                     GNSIPLSQDFRKKYMR"
BASE COUNT         1124 a          748 c          534 g         1319 t
ORIGIN      
        1 ataactcatc aatctcttct acaatctgct cattaaaaaa ataatctaca ctaactttta
       61 acttcttaga cagagcatat aaaaagtctg ctcctggagt aaattctcca ttctctaatc
      121 gactaatctg tccctgttta catatcccct cagtcaattc tcgctgagac atttttaatt
      181 cttttcgtct attttttaat ctggttgcta atagtgtgcc caagtttgca ttttcctttt
      241 ctattcgatt tcaaaagata caaattatat ataagcaatg actagatagg ttatttttga
      301 aaacctatcc cttcttcttt tgaaattttc ttactcaaac gattgataaa cttattcaat
      361 ttaaatacga tgttctcata tctatcgctc tctaattcta tttggatacc taactcattc
      421 aactccttct caacaacatc aatgccaatt ttatcagtta tattcaactt tgaaagattc
      481 attttcattt cttgcttatt ttcaactgaa gccagtaaat attctgctac ttgatttaag
      541 gttgtcactc gttcttgaaa agcattgatt ctcaattcag aagctgctaa ttcatgaact
      601 agttcatctt taattgtaat ataagagtgc ttatccgtat ccagctcaag taaagtatac
      661 acttcctcaa tcttctcctt tatcatctcc acagttgttt tcctgcggaa cggaattgtt
      721 tgattttcag agctgaattg gcgaattaat gttctacctt tcatatagca atttttctcg
      781 gcatcatctt tgtaatagac atagtaggag ctcgtttctc tgataaaaac cttcacctta
      841 tcctcctcca tatcgagctc catgttaggc aaaaagataa gcccttcctg acgaatacca
      901 aacttgacct tgatgtaaat accatcatcc actacttttt caatttgatt gagtgacaac
      961 tctacctcaa attcatgaat agcatctcta tttctcctga actcttgata ggactcccaa
     1021 aacttctcct cgcttgggag ttctttttct tttgagaatc tttcttcttg ataaacttga
     1081 actaaatcct gaacttctgg agctttcaaa tcttttctct tctcaaaata atctttgaaa
     1141 aactctacat catataagtt tttcttgtct agttctgttt ctttcacttc tactcctgca
     1201 aattgaaaag aaacatgctt ttgcttagga ttgatattta atccaaatag ttttgctttt
     1261 ttaatcagat cttctaagtt atcaaccttg ggtaaaataa attccataag ctcttccatt
     1321 ttcctttttg cgaagtaatt cttgaaaaat tcttctgtgt aagcttgctt gtgattgagt
     1381 ttattaccac gcaccacttg tctcatagtt gagtccgtca taaaaaaggt agcatgcttg
     1441 tgactgaaat ccatcttcac atgtagtaat ggagcgttct ttttgaaatc ctcaaaatcg
     1501 gtagaatttt ccatcaaaaa atagagtcgt tgcttaagtt catacttgtg attggtctta
     1561 cgataaactt tatactgatg gtaagaatag cgattctcaa taatttttgc acctgcaatt
     1621 ttagaaaatc tatctgaaat catgcgaaga tttcgttcga ctttataatt ccactttagt
     1681 tttttgtccg aattgttatc aaccgaatta acaatgatat gattatggaa atgattttta
     1741 tcaacgtgag tcgcaacgat aaaacgaaat ttaccaccag ttaactcctt cattgtctca
     1801 tagccaatct gattgatttg tttaggagtg agatgatctt cgggtgaaaa ggactgaatg
     1861 atatgatgag catgaattag tcgttgattt tcctcatgcc tgtcgtggcg aaaatcgtaa
     1921 agcgtatcat tactgataaa attttcatga tacatctgca ccatctcctc ataactagga
     1981 aaatccagaa aattcctcat gccatagtgc gataccaatg caagattttc ggtcttctca
     2041 ggattgagaa tatacttgat aatttttttg cgataacact ttccgtgaac ggcaaaatgc
     2101 ttagtgatga ccatgaaact ccctcaactt tttagcttga atactaaact ctttctctac
     2161 ctctcttatc aagtcagcga ttcccttttt caagtcttgc aattcttcac ttgaaatcaa
     2221 ctgagattga ttgacacttc gggcaatctg gttgatgtta ttaccaatcc tcttcaactc
     2281 aaatactaaa tcttgataac cagttgtatc aatggtgatg aaattcatac cagggtcaag
     2341 tagagttcgt cgagcatatt ctgaaaaaga ttgacaaccg ctttggtcga tacaatgttt
     2401 caactgaccc aattcgctat cagacaggaa aactttcttt agattggttc ggtaacgatt
     2461 ttccatgtgc ctacctcatg tattttttac ggaagtcttg actaagagga atggaattcc
     2521 ctatttcttg aatcaattcc tgaacgcagg ttaagagaat ggatacgtgt tcttgggtga
     2581 cttgatgttc tgcccttgat aagattaaaa tctcataaac atcacgactg atttgtttca
     2641 acttttgagc tcgccagaag gtgaaccact tttcagcagt catttctaac tcgttctcca
     2701 gtaacttgtt tctggcaaat gaagaaaagt gttcaaatcc tttatttttc atcattacgc
     2761 caatcatttc taattcattt gcggtcaaat taacctcttt gcgaatgtct ctgtattgtt
     2821 ctggcatact acttcctcac ttttatctat tgtcggataa ttcccgaccc cgtgtcaacc
     2881 acgggaaact tataaaaaca aaatctatca tcttgtaagg ctttgcgagc tcagatattg
     2941 tgtccacaat atcccaaaaa tcatatcgcc agctaatcaa aacctaccag ttttgacagc
     3001 taacgataag ataacttggt ggctccgccc ccaaaccccc agtgaagatt tgaggtttta
     3061 ttttcttaag aatgatataa gaatagcaga gaaaatttgg aaaagatatc acaggagaat
     3121 gagaggatga aaaaaagaga gcaaatgctc tcaatgaact catataaata tatatcaaaa
     3181 tactcttcaa aaacataagg ttctaaagat tatgtagaaa aagctttagc aagtcttaaa
     3241 cactctgttg ctctaactta tactggtcga tgctaaaata atttaattcc ttgaagtaat
     3301 tttccagctt ttaaatgata ctcttgattt ttcatctcct ctaaacgact atctacagct
     3361 aaggcaaata tattccatat tacattccat cttcgtatct tgttcttgtc atataatctt
     3421 aaatgtcata gagcatggat ttcatatttt gataattagg tattcctacc aatgctcgac
     3481 tagctctatt tatcccttca acataaccct ttgagtcatt caatatacca aaattccaga
     3541 accttttaga attattcggt gtctagttgt aaccgtaata ctcgtaaaat ggctaatgta
     3601 gtacaatcat cttcattttt tatttttcaa ctcctgcact attaaaatca atgtgccaat
     3661 taattgcaga acctaaaaca cctgtaatat caaattgcaa tctatcttga tttagactag
     3721 tcaaa
//
LOCUS       DAVKGC010000131         3709 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKGC010000131 DAVKGC010000000
VERSION     DAVKGC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.131"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..384
                     /locus_tag="VW049_001886"
     rRNA            <1..384
                     /locus_tag="VW049_001886"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            436..508
                     /locus_tag="VW049_001887"
     tRNA            436..508
                     /locus_tag="VW049_001887"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:469..471,aa:Ala,seq:tgc)
     gene            633..3534
                     /locus_tag="VW049_001888"
     rRNA            633..3534
                     /locus_tag="VW049_001888"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3611..>3709
                     /gene="rrf"
                     /locus_tag="VW049_001889"
     rRNA            3611..>3709
                     /gene="rrf"
                     /locus_tag="VW049_001889"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1054 a          759 c         1081 g          815 t
ORIGIN      
        1 tgccggtaat aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc
       61 tgggctacac acgtgctaca atggctggta caacgagtcg caagccggtg acggcaagct
      121 aatctcttaa agccagtctc agttcggatt gtaggctgca actcgcctac atgaagtcgg
      181 aatcgctagt aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca
      241 ccgcccgtca caccacgaga gtttgtaaca cccgaagtcg gtgaggtaac cgtaaggagc
      301 cagccgccta aggtgggata gatgattggg gtgaagtcgt aacaaggtag ccgtatcgga
      361 aggtgcggct ggatcacctc ctttctaagg ataaggaact gcgcattggt cttgtttagt
      421 cttgagaggt cttgtggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag
      481 gtcagcggtt cgatcccgct aggctccatt ggtgagagat caccaagtaa tgcacattga
      541 aaattgaata tctatatcaa atagtaacaa gaaaataaac cgaaaacgct gtagtattaa
      601 taagagttta tgactgaaag gtcagaaaat aaggttaagt taataagggc gcacggtgga
      661 tgccttggca ctaggagccg acgaaggacg tgacaaacga cgatatgcct tgggtagctg
      721 taagtaagcg atgatccagg gatttccgaa tgggggaacc caacaggtaa tacctgttac
      781 ccacatctgt taaggatgtg aggaggaaga cgcagtgaac tgaaacatct aagtagctgc
      841 aggaagagaa agcaaaagcg attgccttag tagcggcgag cgaaacggca gaagggcaaa
      901 ccgaagagtt tactcttcgg ggttgtagga ctgcaatgtg gactcaaaga ttatagaaga
      961 atgatttggg aagatcagcc aaagagagta atagcctcgt atttaaaata gtctttgtac
     1021 ctagcagtat cctgagtacg gcgggacacg tgaaatcccg tcggaatctg ggaggaccat
     1081 ctcccaaccc taaatactcc ctagtgaccg atagtgaacc agtaccgtga gggaaaggtg
     1141 aaaagcaccc cgggagggga gtgaaataga acctgaaacc gtgtgcctac aacaagttcg
     1201 agcccgttaa tgggtgagag cgtgcctttt gtagaatgaa ccggcgagtt acgttatgat
     1261 gcgaggttaa gttggagaga cggagccgta gggaaaccga gtctgaatag ggcgccttag
     1321 tatcatgacg tagacccgaa accatgtgac ctacccatga gcaggttgaa ggtgcggtaa
     1381 gacgcactgg aggaccgaac cagggcacgt tgaaaagtgc ttggatgact tgtgggtagc
     1441 ggagaaattc caaacgaact tggagatagc tggttctctc cgaaatagct ttagggctag
     1501 cgtcgacatt agagattctt ggaggtagag cactgtttgg gtgaggggtc catcccggat
     1561 taccaatctc agataaactc cgaatgccaa tgaattatgg tcggcagtca gactgcgagt
     1621 gctaagatcc gtagtcgaaa gggaaacagc ccagaccacc agctaaggtc ccaaaataat
     1681 tgttaagtgg aaaaggatgt ggggttgcac agacaactag gatgttagct tagaagcagc
     1741 tattcattca aagagtgcgt aatagctcac tagtcgagtg accctgcgcc gaaaatgtac
     1801 cggggctaaa acaatttacc gaagctgtgg atacctttat aggtatggta ggagagcgtt
     1861 ctatgtgtga tgaaggtata ccgtgaggag tgctggaacg catagaagtg agaatgccgg
     1921 tatgagtagc gaaagacagg tgagaatcct gtccaccgta agactaaggt ttccagggga
     1981 aggctcgtcc gccctgggtt agtcgggacc taaggagaga ccgaaaggtg tatccgatgg
     2041 acaacaggtt gatattcctg tactagagta tgtagtgatg gagggacgca gtaggctaac
     2101 taaagcagac gattggaaga gtctgtctaa gcagtgaggt gtgaattgag tcaaatgctt
     2161 aattctataa cattgagctg tgatggggag cgaagtttag tagcgaagtt agtgacgtca
     2221 cactgccaag aaaagcttct agcgtttaaa catactctac ccgtaccgca aaccgacaca
     2281 ggtagtcgag gcgagtagcc tcaggtgagc gagagaactc tcgttaagga actcggcaaa
     2341 atgaccccgt aacttcggga gaaggggtgc tgacttaaag tcagccgcag tgaataggcc
     2401 caagcaactg tttatcaaaa acacagctct ctgctaaatc gtaagatgat gtataggggg
     2461 tgacgcctgc ccggtgctgg aaggttaaga ggagtgctta gcgtaagcga aggtatgaat
     2521 tgaagcccca gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt
     2581 cgggtaagtt ccgacccgca cgaaaggcgt aatgatttgg gcactgtctc aacgagagac
     2641 tcggtgaaat tttagtacct gtgaagatgc aggttacccg cgacaggacg gaaagacccc
     2701 atggagcttt actgcagttt gatattgagt gtctgtacca catgtacagg ataggtagga
     2761 gtctaagaga tcgggacgcc agtttcgaag gagacgctgt tgggatacta cccttgtgtt
     2821 atggccactc taacccagat aggtgatccc tatcggagac agtgtctgac gggcagtttg
     2881 actggggcgg tcgcctccta aaaggtaacg gaggcgccca aaggttccct cagaatggtt
     2941 ggaaatcatt cgcagagtgt aaaggtataa gggagcttga ctgcgagagc tacaactcga
     3001 gcagggacga aagtcgggct tagtgatccg gtggttccgt atggaagggc catcgctcaa
     3061 cggataaaag ctaccctggg gataacaggc ttatctcccc caagagttca catcgacggg
     3121 gaggtttggc acctcgatgt cggctcgtcg catcctgggg ctgtagtcgg tcccaagggt
     3181 tgggctgttc gcccattaaa gcggcacgcg agctgggttc agaacgtcgt gagacagttc
     3241 ggtccctatc cgtcgcgggc gtaggaaatt tgagaggatc tgctcctagt acgagaggac
     3301 cagagtggac ttaccgctgg tgtaccagtt gtcttgccaa aggcatcgct gggtagctat
     3361 gtagggaagg gataaacgct gaaagcatct aagtgtgaaa cccacctcaa gatgagattt
     3421 cccatgatta tatatcagta agagccctga gagatgatca ggtagatagg ttagaagtgg
     3481 aagtgtggcg acacatgtag cggactaata ctaatagctc gaggacttat ccaaagtaac
     3541 tgagaatatg aaagcgaacg gttttcttaa attgaataga tattcaattt tgagtaggta
     3601 ttactcagag ttaagtgacg atagcctagg agatacacct gtacccatgc cgaacacaga
     3661 agttaagccc tagaacgccg gaagtagttg ggggttgccc cctgtgaga
//
LOCUS       DAVKGC010000132         3658 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKGC010000132 DAVKGC010000000
VERSION     DAVKGC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.132"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            158..1387
                     /gene="arcA"
                     /locus_tag="VW049_001890"
     CDS             158..1387
                     /gene="arcA"
                     /locus_tag="VW049_001890"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9432947.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1445..2461
                     /gene="argF"
                     /locus_tag="VW049_001891"
     CDS             1445..2461
                     /gene="argF"
                     /locus_tag="VW049_001891"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9432948.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2626..3573
                     /gene="arcC"
                     /locus_tag="VW049_001892"
     CDS             2626..3573
                     /gene="arcC"
                     /locus_tag="VW049_001892"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9432949.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIILTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
BASE COUNT         1161 a          712 c          761 g         1024 t
ORIGIN      
        1 tgttgtgtat aataacataa aattgtgcta aatatcataa tttatattat tttccgagaa
       61 attatagcca ttgacattac ctaggaattg cgttataaca tacatgtaag cggtacccaa
      121 aatggtgcca agtcaaaatt tttaaggagg aaaatacatg tcttcacatc caattcaggt
      181 cttctcagaa attgggaaac tgaaaaaagt tatgttgcac cgtccaggca aggagttaga
      241 aaacttgttg ccggactatc ttgaaaggct tctttttgat gatattcctt tcttggaaga
      301 tgctcaaaaa gaacatgatg catttgccca agctcttcgc gatgaaggaa ttgaggttct
      361 ctacctagaa caactcgctg ctgaatcatt gacctctcca gaaatccgcg atcaatttat
      421 cgaggaatac ttagacgaag ccaacatccg tgatcgtcaa accaaggttg ctattcgtga
      481 attgcttcac ggcatcaagg acaaccaaga attggttgaa aaaacaatgg ctgggattca
      541 aaaagttgaa ttgccagaaa ttcctgacga agctaaagat ctaactgact tagttgaatc
      601 agattatcca tttgcaattg acccgatgcc aaacctctat ttcactcgcg acccatttgc
      661 aacaattgga aacgccgtat cgcttaacca catgtttgca gacactcgta accgtgaaac
      721 actctacggt aagtatatct tcaaatacca cccaatctat ggcggaaaag tggatttggt
      781 ctacaaccgt gaagaagata cgcgtatcga aggtggagac gagctagttc tttctaaaga
      841 cgtccttgca gtaggtattt ctcaacgtac agacgcagct tctatcgaaa aacttttggt
      901 caacatcttc aagaaaaatg ttggcttcaa gaaagttttg gcctttgaat ttgctaacaa
      961 ccgtaaattc atgcacttgg atactgtctt cactatggta gactatgaca agttcactat
     1021 tcacccagaa atcgaaggcg accttcacgt ttactcagtt acttacgaaa acgaaaaact
     1081 taaaatcgtt gaagagaaag gtgacttagc tgaacttctt gctcaaaacc ttggtgtaga
     1141 aaaagttcat ttgattcgtt gcggtggtgg caatatcgta gcagctgcgc gtgaacaatg
     1201 gaacgacggt tctaacactt tgaccatcgc acctggtgtg gtagttgttt atgaccgcaa
     1261 taccgtgacc aataagattt tggaagaata cgggcttcgc ttgattaaga ttcgcggaag
     1321 tgaattggtt cggggccgtg gtggacctcg ttgtatgtct atgccatttg aacgtgaaga
     1381 agtgtaatcg ctgttcggca ttcgtcaata gaaaatgtaa aaaatagaaa gaggaaataa
     1441 taaaatgaca aattcagtat tccaaggacg cagcttctta gcagaaaaag actttacccg
     1501 tgcagagtta gaatacctta ttggtctttc agctcacttg aaagatttga aaaaacgcaa
     1561 tattcaacac cactaccttg ctggcaagaa tatcgctctc ctatttgaaa aaacatctac
     1621 tcgtactcgt gcagccttta caactgcggc tatcgacctt ggtgctcacc cagaatacct
     1681 cggagcaaat gatattcagt tgggtaaaaa agaatctact gaagatactg ctaaagtatt
     1741 gggacgtatg tttgacggga ttgaattccg cggattcagc caacgtatgg ttgaagaatt
     1801 ggcagaattc tcaggcgttc cagtatggaa cggtctaact gacgaatggc acccaactca
     1861 aatgctcgct gactacttga ctgttcaaga aaacttcggt cgcttggaag gcttgacatt
     1921 ggtatactgt ggtgatggac gtaacaacgt tgccaacagc ttgctcgtaa caggtgctat
     1981 ccttggtgtc aatgttcaca tcttctcacc aaaagaactc ttccccgaaa aagaaatcgt
     2041 tgaattggca gaaggatttg ctaaagaaag tggcgcacat gttctcatca ctgaagatgc
     2101 tgatgaagca gttaaagatg cagacgttct ttacacagac gtttgggtat caatgggtga
     2161 agaagacaaa ttcgcagaac gtgtagctct tcttaaacct taccaagtca atatggactt
     2221 agttaaaaaa gcaggcaatg aaaacttgat cttcctacac tgcttgccag cattccacga
     2281 tactcacact gtttatggta aagacgttgc tgaaaaattt ggtgtagaag aaatggaagt
     2341 aacagacgaa gtcttccgca gcaagtacgc tcgccacttc gatcaagcag aaaaccgtat
     2401 gcacactatc aaagctgtta tggctgcgac acttggtaac ctttatattc ctaaagtata
     2461 attttagata ataaaccgtc taccaacagc tatgagggct gcgactaata gctttagtcc
     2521 ggtcctcttt tatgtaatgg taatctatta tttcttataa aatatgtgaa aaatcattaa
     2581 attgaaatct aaacgcattc tattgagtgt gataaaggag aatttatggc aaatcgtaaa
     2641 attgtagtag ctttgggagg aaatgcgatt ctttcttctg acccatcagc aaaggctcaa
     2701 caagaagctt tagttgaaac agctaagcat cttgtaaaat tgattaaaaa tggagatgat
     2761 ttgattatca ctcacggtaa tggacctcaa gttgggaatc tcttgctcca acatttggca
     2821 tcagactctg aaaagaaccc tgccttccca ctcgactcac ttgtcgctat gacagaaggt
     2881 agcatcggtt tctggttgaa aaatgctttg caaaatgctc tcttggatga aggcatcgaa
     2941 aaaaatgttg cctctgttgt aacgcaagtt gtcgtagata aaaatgatcc agcttttgtt
     3001 aacttgagta aaccaatcgg tcctttctat tcagaagaag aagcaaaagc agaagccgaa
     3061 aaaagcggag cgactttcaa ggaagatgct ggccgtggct ggcgtaaggt cgttgcctca
     3121 ccaaaacctg ttgacatcaa agaaattgaa accatccgta ctcttttaaa taatggtcaa
     3181 gtcgtcgtag ctgcaggtgg tggcggtatt cccgtcgtca aagaaaacaa tggacatttg
     3241 actggtgtcg aagcggttat tgataaagac ttcgcttccc aacgtttggc agaattggtt
     3301 gatgcagacc tcttcatcat tttgacaggt gtagattatg tatttgttaa ctacaacaag
     3361 ccaaaccagg aaaaattgga acatgtgaat gttgcccagc tggaagaata tatcaaacaa
     3421 gatcagtttg caccaggtag catgcttcca aaagtagaag cagctatcgc ttttgtcaat
     3481 ggtcgtccag aaggaaaagc agttattact tcccttgaaa atctaggcgc cttgattgaa
     3541 tctgaaagtg gaacaattat tgaaaaagga taagttgttt tactaataag atgtattcta
     3601 tttctagtat ctttatatca aattagaaat tattcttgaa aacatgtaca atatttca
//
LOCUS       DAVKGC010000133         3650 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKGC010000133 DAVKGC010000000
VERSION     DAVKGC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.133"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            103..1512
                     /locus_tag="VW049_001893"
     CDS             103..1512
                     /locus_tag="VW049_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9432950.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKKEYGNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKRS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1530..2825
                     /locus_tag="VW049_001894"
     CDS             1530..2825
                     /locus_tag="VW049_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9432951.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2830..3138
                     /locus_tag="VW049_001895"
     CDS             2830..3138
                     /locus_tag="VW049_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9432952.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALEWIGEIR
                     "
     gene            3135..3443
                     /locus_tag="VW049_001896"
     CDS             3135..3443
                     /locus_tag="VW049_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9432953.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1238 a          612 c          707 g         1093 t
ORIGIN      
        1 gaggcaaaca caagatatgt atcaacaatt tttaaaaatg tctagacaaa ttttaaaatt
       61 atgctatact ttatgtatag aataatagaa aggaggttga gcatgaaaaa aaagttagta
      121 tttcctaatc tgttttggtg gggagctgct tctagcggac ctcagacaga aggtcaatat
      181 ggaaaagtac atgaaaatgt gatggactac tggttcaaaa cgcatccaga agattttttc
      241 gataatgtcg gacctcttgt agccagtaac ttttttcata cttacaccga agatttccac
      301 ttgatgaagg aaattggagt taattctttc cgcacttcca tccaatggag tcgactcatc
      361 aagaatttag agacaggtga gcctgatcca aaaggtattg ctttctacaa tgccatcatt
      421 gaagaagcta aaaagaacca gatggatctt gtgatgaatt tacatcattt tgatttacca
      481 gtggaacttc ttcaaaaata cggtggttgg gaaagcaaac atgtagtgga gttattcgtg
      541 aagtttgcca agactgcttt cacatgcttt ggagataagg ttcattactg gacaactttc
      601 aatgagccaa tggtcattcc agaagcagga tacttatatg ctttccatta tccaaatcta
      661 aaaggaaagg gaaaagaggc cgtacaagtc atctataatc taaaccttgc tagtgcaaaa
      721 gtgattcaac tatatcgctc attaggactt gatggaaaga ttgggattat tttaaacttg
      781 acacctgctt atccaagaag taattctcca gaagacttag aagcaagtcg atttacagat
      841 gacttcttta acaaagtctt cttgaatcca gctgttaaag gaactttccc agaaagattg
      901 gtaaaacagc tagagagaga tggcgtgtta tggagtcata ccgaaaaaga gcttcaactg
      961 atgaaatcaa atacggttga ttttcttgga gtaaactact accatccaaa acgtgttcaa
     1021 gcacaagcaa atcctgagga atatcagacg ccctggatgc cagaccaata cttcaaagag
     1081 tatgaatggc cggagcgtcg catgaatcca tatcgtggtt gggaaatttt tccgaaagcc
     1141 atttatgata ttgctatgat tgtgaagaaa gaatatggta atattccatg gtttatcagt
     1201 gaaaacggaa tgggtgttga aaacgaagca cggtttatcg gtgaaaatgg agttatcgat
     1261 gacgtgtatc gtattgaatt ttatgaagaa catttaagat ggctacataa agccattgaa
     1321 gagggaagtc actgttttgg ataccacgct tggaccgcat ttgattgctg gtcttggaat
     1381 aatgcatata agaatcgtta cggatttatc tccgttgatt tagaaacgca aaagagaacc
     1441 atcaagagat caggaagatg gtatcgcaaa gtaagtgaca ataacggttt tgaagtagaa
     1501 attgaggagt aaagagagga gatattcaga tgaaaaactc aaaatttata gatcaatttg
     1561 ccacctttgc tggtaaacta gggaaccaaa ttcatttaaa aaccctaaga gatgcattcg
     1621 taacagtaat gccattatat attttggcag gtttaatcgt tcttttgaac aacacggtat
     1681 ttaagtggat tttccaaggg gatacattaa caagattcca atattgggga ataacaattg
     1741 caaacggtac tttaagtatt tcaggtatga ttattgctgt aatggttggt tatttcttag
     1801 ctaaaaacag agatttcgaa aacccgttag cagcatcaat gctatcatta gtttctttaa
     1861 ttgtgatgat gccaaataca gtttctgtag ttcctgacgg agcaaaagat gcggtaaaca
     1921 tttcaggtgt tctttcattc aacaacacag gtacaggcgc aatgttcgcc ggggttatcg
     1981 tagcgattat tgcaacagaa ttattcattg aattatcaaa cgttaaagct ttacaaatga
     2041 accttggtga aaatattcca ccagctgtta gtagatcatt tagcgtatta cttccagtca
     2101 tgaccgtcat ctccttattt ggggttgttt cagcattatt attcaatata actggaatga
     2161 acttaatctc aatcattaca atctttattc aagaaccaat tcgtcatatt ggtacaagct
     2221 taatcggggt cattattatt tactctttag gaaatatgtt atggctattt ggtattcacc
     2281 aagcagttat ttacagtgcc atcctagaac cattactatt aattaacatt actgaaaaca
     2341 tcactgcagc aaataatgga caagccattc cacacatcat caacttatca caaatacaaa
     2401 cattcgcttt aatgggtggt agtggatcta cattatgttt attaatagca acattcttag
     2461 taagtcgcaa tgctgtttct aaaaacgtgg ctaaattatc ttttggacct ggtatcttca
     2521 atatcaatga accagtatta ttcggttacc caatcgttta taacatttca ttagctattc
     2581 catttatcac agttccagta cttggtattt taatcagcta cttagcaaca gttacaggat
     2641 tcatgagtcc tgcatttata caagttcctt ggactacacc agtattctta aatgcatggt
     2701 tagcaacagc aggggacgtg agagcagttc tagttcaatt catcatcttt gcacttggag
     2761 ttcttctata cattccattt atcaaagtta atgacaaagt tgttgaacaa gaaatggaag
     2821 gttaagatta tgaaacaaat tttattagta tgtaatgcgg gaatgtcgac aagtatgctt
     2881 gttaaaaaaa tgcaacaaag tgcgactgaa cgtgggattg aaatttctat ccaagcaaaa
     2941 tcaatgacag aagctaaaaa aaatatccat gaagcagatg tcattctaat tgggccacaa
     3001 attcgttatg agttacttgc tgtgaaagaa attgcaggaa atatccctgt agataccatt
     3061 gatatgcggg attacggaat gatgaatgga gcaaaagttc tggaacaagc acttgaatgg
     3121 ataggagaaa taagatgaca gaagaaatgg aatatatctg ttttcagctc attgccaata
     3181 gtggagcagc caaatcatcc tttattgaag ccatccaact agctaaggca ggaaatttaa
     3241 aagaagcgaa aattaaagtt gaagaagcgg aagactcctt agtagaagcg cacaagattc
     3301 actccaatct catacaaaaa gaagctactg gagaaaaaat aggattctcc ttgctattta
     3361 tgcatgcaga agaccaaatg gcttcaacag agattattca gttgctatct aaagaattca
     3421 tcgatctcta tcaaaataaa taggaacact gtcgttccca tctgtcttta ttaatgacga
     3481 ctacattgta ggaacctcca aaagttggac aaaatgattg aaaggattgg atcctgcatt
     3541 acacaagatc caatcctttt cttttactct agtttttata atatgttgaa tctagaatag
     3601 tccactgcag tttctaaaaa attgctggaa atagatgtta agctccaatt
//
LOCUS       DAVKGC010000134         3626 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKGC010000134 DAVKGC010000000
VERSION     DAVKGC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.134"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            78..305
                     /locus_tag="VW049_001897"
     CDS             78..305
                     /locus_tag="VW049_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432954.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            593..3475
                     /gene="nanA"
                     /locus_tag="VW049_001898"
     CDS             593..3475
                     /gene="nanA"
                     /locus_tag="VW049_001898"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU9432955.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNNQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGVIG
                     LEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKGSIESM
                     HNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLVTLTTK
                     EDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1231 a          611 c          820 g          964 t
ORIGIN      
        1 attgctgtaa aaatagtagt agctatgcga aataacagat agagagaagg gattgaagct
       61 tagaaaaggg gaataatatg atatttaagg cattcaagac aaaaaagcag agaaaaagac
      121 aagttgaact acttttgaca gtttttttcg acagttttct gattgattta tttcttcact
      181 tatttgggat tgtccccttt aagctggata agattctgat tgtgagcttg attatatttc
      241 ccattatttc tacaagtatt tatgcttatg aaaagctatt tgaaaaagtg ttcgataagg
      301 attgagcagg aagtatggtg taaatagcat aggctgatgt ccatcatttg cttataaaga
      361 gatattttag tttaattgca gcggtgtcct ggtagataaa ctagattggc aggagtctga
      421 ttggagaaag gagaggggaa aattggcacc aatttgagat agtttgttta gttcattttt
      481 gtcatttaaa tgaactgtag taaaagaaag ttaataaaag acaaactaag tgcattttct
      541 ggagtaaatg tcttatttca gaaatcggga tatagatata gagaggatca gtatgaatcg
      601 gagtgttcaa gaacgtaagt gtcgttatag cattaggaaa ctatcggtag gagcggtttc
      661 tatgattgta ggagcagtgg tatttggaac gtctcctgtt ttagctcaag aaggggcaag
      721 tgagcaacct ctggcaaatg aaactcaact ttcgggggag agctcaaccc taactgatac
      781 agaaaagagc cagccttctt cagagactga actttctggc aataatcaag aacaagaaag
      841 gaaagataag caagaagaaa aaattccaag agattactat gcacgagatt tggaaaatgt
      901 cgaaacagtg atagaaaaag aagatgttga aaccaatgct tcaaatggtc agagagttga
      961 tttatcaagt gaactagata aactaaagaa acttgaaaac gcaacagttc acatggagtt
     1021 taagccagat gccaaggctc cagcattcta taatctcttt tctgtgtcaa gtgctactaa
     1081 aaaagatgag tacttcacta tggcagttta caataatact gctactctag aggggcgtgg
     1141 ttcggatggg aaacagtttt acaataatta caacgatgca cccttaaaag ttaaaccagg
     1201 tcagtggaat tctgtgactt tcacagttga aaaaccgaca gcagaattgc caaatggtcg
     1261 agttcgcctg tatgttaatg gtgttttatc tcgaacaagt ctgaaatctg gcaatttcat
     1321 taaagatatg ccagatgtaa cgcatgtgca aatcggagca accaagcgtg ccaacaatac
     1381 ggtttggggg tcaaatctac agattcggaa tctcactgtg tataatcgtg ctttaacacc
     1441 agaagaggta caaaaacgta gtcaactttt taaacgctca gatttagaaa aaaaactacc
     1501 tgaaggagcg gctttaacag agaaaacgga catattcgaa agcgggcgta acggtaaacc
     1561 aaataaagat ggaatcaaga gttatcgtat tccagcactt ctcaagacag ataaaggaac
     1621 tttgatcgca ggtgcagatg aacgccgtct ccattcgagt gactggggtg atatcggtat
     1681 ggtcatcaga cgtagtgaag ataatggtaa aacttggggt gaccgagtaa ccattaccaa
     1741 cttacgtgac aatccaaaag cttctgaccc atcgatcggt tcaccagtga atatcgatat
     1801 ggtgttggtt caagatcctg aaaccaaacg aatcttttct atctatgaca tgttcccaga
     1861 agggaaggga atctttggaa tgtcttcaca aaaagaagaa gcctacaaaa aaatcgatgg
     1921 aaaaacctat caaatcctct atcgtgaagg agaaaaggga gcttatacca ttcgagaaaa
     1981 tggtactgtc tatacaccag atggtaaggc gacagactat cgcgttgttg tagatcctgt
     2041 taaaccagcc tatagcgaca agggtgatct atacaagggt aaccaattac taggcaatat
     2101 ctacttcaca acaaacaaaa cttctccatt tagaattgcc aaggatagct atctatggat
     2161 gtcctacagt gatgacgacg ggaagacatg gtcagctcct caagatatta ctccgatggt
     2221 caaagccgat tggatgaaat tcttgggtgt aggtcctgga acaggaattg tacttcggaa
     2281 tgggcctcac aagggacgga ttttgatacc ggtttatacg actaataatg tatctcactt
     2341 aaatggctcg caatcttctc gtgtcatcta ttcagatgat catggaaaaa cttggcatgc
     2401 tggagaagcg gtcaacgata accgtcaggt agacggtcaa aagatccact cttctacgat
     2461 gaacaataaa cgtgcgcaaa atacagaatc aacggtggta caactaaaca atggagatgt
     2521 taaactcttt atgcgtggtt tgactggaga tcttcaggtt gctacaagta aagacggagg
     2581 agtgacttgg gagaaggata tcaaacgtta tccacaggtt aaagatgtct atgttcaaat
     2641 gtctgctatc catacgatgc ataatggaaa agaatacatt atcctcagca atgcaggtgg
     2701 accgaatcgt gaaaatggga tggtccactt ggcacgtgtc gaagaaaatg gtgagttgac
     2761 ttggctcaaa cacaatccaa ttcaaaaagg agagtttgcc tataattcgc tccaagaatt
     2821 aggaaatggg gagtatggca tcttgtatga acatactgaa aaaggacaaa atgcctatac
     2881 cctatcattt agaaaattta attgggaatt tttgagcaaa gatctgattt ctcctaccga
     2941 agcgaaagtg aagcgaacta gagagatggg caaaggagtt attggcttgg agtttgactc
     3001 agaagtattg gtcaacaagg ctccaaccct tcaattggca aatggtaaaa cagcgacttt
     3061 cctaacccag tatgatagca aaaccttgtt gtttgcagta gataaggaag atatcggaca
     3121 ggaaattatt ggtatagcta aaggaagcat cgaaagtatg cataatcttc ctgtaaatct
     3181 agcaggtgcc agagttcctg gcggagtaaa tggtagcaaa gcagcggtgc atgaagttcc
     3241 agaatttaca gggggagtta atggtacaga gccagctgtt catgaaatcg cagagtataa
     3301 gggatctgat tcgcttgtaa ctcttactac aaaagaagat tatacttaca aagctcctct
     3361 tgctcagcag gcacttcctg aaacaggaaa caaggagagt gacctcctag cttcactagg
     3421 actaacagct ttcttccttg gtctgtttac gctagggaaa aagagagaac aataagagaa
     3481 gaattctaaa catttgattt tgtaaaaata gaaggagata gcaggttttc aagcctgcta
     3541 tctttttttg atgacattca agctgatacg aaatcataag aggtctgaaa ctactttcag
     3601 agtagtctgt tctataaaat atagta
//
LOCUS       DAVKGC010000135         3606 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKGC010000135 DAVKGC010000000
VERSION     DAVKGC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.135"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(132..1268)
                     /gene="lctO"
                     /locus_tag="VW049_001899"
     CDS             complement(132..1268)
                     /gene="lctO"
                     /locus_tag="VW049_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9432956.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKDEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1892..3382)
                     /gene="lysS"
                     /locus_tag="VW049_001900"
     CDS             complement(1892..3382)
                     /gene="lysS"
                     /locus_tag="VW049_001900"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9432957.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHFQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3510..>3606)
                     /locus_tag="VW049_001901"
     CDS             complement(3510..>3606)
                     /locus_tag="VW049_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9432958.1"
                     /translation="YDLEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
BASE COUNT         1039 a          773 c          700 g         1094 t
ORIGIN      
        1 ttagaaatcg atttaactgt cctgattaat ttgtcctatt cttatttcat tttattatag
       61 ttttcgatag cgatttattc ttccaataca cgaagaaaaa cctccacatt cagtggaggc
      121 aatctgtttt atcaatacaa ttttaagtca cgagggtcaa ctgggaaggt tgggttgtat
      181 ggattgtgac ggagcttaaa gtgtttgaca tcttcaatgg tctgagctcc agacaattgc
      241 ataactgtct tcaattccgc attcaagtgt tcaaagactt gacgcacacc gacactacca
      301 ccgagagcca agccataaat aacagggcga ccaatagcta ccaagtctgc tcctgaagcc
      361 aaggctttaa agacgtgttg accacgacga acaccagagt caaagacaat cggcacacgt
      421 ctatcaactg cttctgccac ttcttgaagc gagtcaaagg cagctggtcc accgtcgatt
      481 tgacgaccac cgtggttggt tacccagata ccagaagctc ctgcagcaag cgaacgttca
      541 acgtcctcac ggcattgtgg tcccttgaca tacacaggaa gaccagagta ttcagcgata
      601 aattctacat cgcgtggaga caagcgttgt ttagctgatt tgtaaacaaa gtccattgat
      661 ttaccagcac cttctggcag gtattcttca acaatcggca tgccaactgg gaagacaaaa
      721 ccattacgct tatccacttc acgattcccc cctacagtag catctgccgt caagacaatc
      781 gctttataac cttcatcctt cacacggtcc atgatgtggc ggttgatacc gtcatcctta
      841 ctaaagtaaa attgaaacca atgaggtgtc ccttgaaggg cttcagaaat ctctggaagg
      901 tcaacagtag agtaagaact ggttgtataa agagaaccaa actcatgcac accacgcgca
      961 gtcgccactt ccccctgttc atttgccaat ttatgagccg caacaggtgc cataatgatt
     1021 ggcgaagata gtttttcacc tgcaaattca atctctgtac ttggattttc aacatcgcaa
     1081 agtgtatgag gaacgatgag tttgtggtta aaggcgcgga tattctctct taaagtgaaa
     1141 gtatcttccg ccccactagc gatatagcca aatgctgctt taggaataac ttgttgcgcc
     1201 attggctcca aatcataggt attgatgaag tctacatgac cttctgcatt gcttgttttg
     1261 tatgacataa actgtcctcc tcgattaagt aagcgtttgc tttttactat acaactataa
     1321 taactctttt ttcaatcatt ttcaagagtt ttgcttaaaa accatcttat tttttcatac
     1381 aatatactag ccctactttc cttaaactct aaaccttagt ctatattaag tgaatcgttg
     1441 agattttttg ctgccaggca tttgaactac ttttttattt gctgattcta tctactaata
     1501 ctataaaaaa caagtgaata agacaccaga acatcctcta ataggaccta tggacttata
     1561 tcagtcccat atatactgtt taagcaagat actatcctag ttgacgctaa cttcctatcc
     1621 cacttattaa ttttttattt atagtgaatc tgaacaaatc aattggggaa tccaagtcaa
     1681 tttctagtac tttttgaaaa gtttcagcgt actatcgtaa aatgaaataa aacatgcaca
     1741 aattgattag ggaatttaaa ccaatttcta acaatgtctt agaaatcaaa gtgtactatt
     1801 tcaatttcaa tacactacac ttcattttct ccttaaattc agtctctttt tgaataaaaa
     1861 aatcctctgg taagagccag aggataagaa tttatttcat tgttgggaag agcaatacat
     1921 cacggatagt tgttgtatca gtgaggagca tgcagagacg gtcgataccg attcccaaac
     1981 cacctgttgg tggcataccg tattcaagag cttcaatgta gtcatagtcg attcctgtcg
     2041 cttcatcatc accaagttct ttggctttag cttgggcttc aaaacggcta agttggtcga
     2101 ttgggtcgtt gagctcagta aaggcattac cgtactcctt agtcatgata aagagctcga
     2161 aacggtcagt aaagcgttgg tcttcaggat ttttcttagc gagtggagat acagctactg
     2221 gatgtccata gacaaaggtt ggttggatta aagtttcttc aacaaactct tcaaagaagg
     2281 cattgatgat gtgaccaacc tcagtgtagt gtttctcaac tggaactttc ttctcagcag
     2341 cgatagcttt agcttcttcc aaagtcatgt cttgccagaa atcgacacca gtaatttctc
     2401 tgatagcatc caccatatga acacgcttaa atggttcgtt aatcttgatt tcagtacctt
     2461 gatagttgac tgggccatcg cccttaactg atttagcagc atgttggata atgccttccg
     2521 tcaagtccat gatatcttgg aagtctgcat aagcttggta aacttcgata gaagtgaact
     2581 cagggttatg agtagcatcc attccttcgt tacggaagat acgcccaatt tcatagacac
     2641 gttccatacc acccacgata aggcgtttta agtgaagctc agtcgcgata cgaagcacca
     2701 tgtcaatgtt ttgggcattg tggtgggtga taaatggacg ggcagcagca ccaccagctt
     2761 cgttgtgaag aacaggtgtt tccacttcaa ggaatccttt ttggtcaagg taacgacgga
     2821 tttcagagat gatttttgaa cgagtgacaa aacgttcaaa gctttcacga ttagaaatca
     2881 agtcaaggta acgtttacgg taaattgttt caacgtctgt caaaccatgg aatttctcag
     2941 gaagaggacg aagagcctta gacaagtgtg tgatgtgggt tgccttgata gagagttctc
     3001 ccatatccgt acgcatcact tcaccttcga caccaaggaa gtcaccaaga tctgcttttt
     3061 tgaagatttc gtagttttct tcaccgacag catccttacg aacgtagatc tgaatctggc
     3121 cttcgcggtc ttgaaagtgg gcaaaaccaa cttttccttt accacgtttg gttatcaagc
     3181 gtcctgcgat agtagctgtt tcgtttttat cgtgtaattg ttctttatcg aggttggcat
     3241 atttatcttt taattcttgt gaatttgcag tacgttcaaa acgttttccg aaaggatcga
     3301 ttccttgttc gcggagcgca gccatttttt cacggcgaac gatctgctgg tcatttagtt
     3361 cttccatatg ttctgtagac atgggatcct cctagtttta ctcaaaattg aatacgatga
     3421 gtcatttttt gcgatactcc cattttatca taaataagca agaaattcac ggataatctt
     3481 taaaaactaa aaagaacctg acgctaaaat cactggaaag aagtgaaagt atctcccgcc
     3541 ccactagcga tatagccaaa tgctgcttta ggaataactt gttgcgccat tggctccaaa
     3601 tcatag
//
LOCUS       DAVKGC010000136         3337 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKGC010000136 DAVKGC010000000
VERSION     DAVKGC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.136"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            7..657
                     /locus_tag="VW049_001902"
     CDS             7..657
                     /locus_tag="VW049_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9432959.1"
                     /translation="MVSCQALEGEPLYTPNGGVMPLLAKAAFQAGAKGIRANSVRDIS
                     EIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILALDCTNRSRPGYDNI
                     TDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYTPYSPKKDNPDFEL
                     VERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPKEIAQRFINVIK"
     gene            682..1599
                     /locus_tag="VW049_001903"
     CDS             682..1599
                     /locus_tag="VW049_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEU9432960.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            1627..3168
                     /locus_tag="VW049_001904"
     CDS             1627..3168
                     /locus_tag="VW049_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU9432961.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAIAIVYSYTGGLKSVLWTDFIQGVVLISGVVVALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLAIATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGVTGL
                     ILAAIYAAAQSTVSTGLNSVATSWTLDIQDVISKNMSEDRRTKIAQFVSLAVGLFSIV
                     VSIIMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWTYSLISISVSVVSGYIVSVLTENKVSAPKYTTIHDISEIKADSS
                     WEVRH"
     gene            3194..>3337
                     /locus_tag="VW049_001905"
     CDS             3194..>3337
                     /locus_tag="VW049_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9432962.1"
                     /translation="MIISEQSDFRRYASVNKHFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFA"
BASE COUNT         1045 a          507 c          664 g         1121 t
ORIGIN      
        1 caaataattg tttcctgcca agcgttagaa ggagagccac tttatactcc aaatggagga
       61 gtgatgccgt tattagcaaa agccgccttt caagcaggcg ctaaaggtat tcgtgctaat
      121 tctgtccgcg atatttcaga aataaaagaa gaagttgact taccaattat aggtatcata
      181 aaaagagatt acgatggatt tgaacctttt atttcagcaa ctatgaaaga gattgatgaa
      241 ttagttagtg aaggagtaga cattttagct ttggattgta ctaatcgttc aagaccaggt
      301 tatgataata ttactgactt tattcatgat attaaagtca agtatccaaa ccaattatta
      361 atggctgata tttctacttt tgaagaaggg aaagtagctg ctgaatcggg agtagatttt
      421 gtgggtacga cattatctgg ttatacacca tatagcccta aaaaagataa tcctgatttt
      481 gagttagttg aaagattggt aaaagagctt gacgtaccag ttattgctga aggaagaata
      541 agtactccgg aacaagctag aaaaatgtta gatttaggtg cttacgcagt ggttgttgga
      601 ggagctatca ctcgtccaaa agaaattgct cagcgattta ttaatgtcat caaataaaaa
      661 acaatataaa ggagaaatct gatgaaagat ttaactaaat acaaaggcgt tattcctgca
      721 ttttatgctt gctatgatga aaatggtgaa attagccaag aacgtgtaaa atctctggtg
      781 caatatttca ttgacaaagg tgtaaaaggt atctatgtaa atggttcttc aggtgaatgt
      841 atttaccaaa gtgtagaaga tcgtaaacaa attattgaag ctgttatgga agttgctaaa
      901 ggtaaattaa cagttatcaa ccatgttgca tgtaataaca cgaaagatag tattgaattg
      961 gcaaaacatt cagaaagtgt tggagtcgat gctattgctg ctatcccacc tatttacttc
     1021 aaattgccag aatattcaat tgcagcatat tggaatgcaa tgagtgaagc tgcgccaaat
     1081 acagatttta tcatttataa tattccacaa ttggcagggg ttgcgttgac tggtagtttg
     1141 tatgcaacaa tgcgtcaaaa tcctcgtgtg attggagtta aaaattcttc tatgcctgta
     1201 caagatattc aaatgtttgt agctgcaggt ggagaagatt acattgtatt caatggtcca
     1261 gatgaacaat atcttggtgg tcgcttgatg ggagcagaag ctggtattgg tggtacttat
     1321 ggcgttatgc cagatttgtt cttgaaattg gaaagtttga ttcaagaacg agatttagat
     1381 actgctaaaa aacttcaata tgctatcaat gaagttatct ataagatgat atcaggtaag
     1441 gcaaatatgt atgctgtagc aaaagaggtt ttgcgtctaa atgaaaaact tgatttaggt
     1501 tctgttcgtc aacccttaga agcattggca gaaggtgact tggaagttgc aaaacaagca
     1561 gcagaactta ttcaacaagc acgaaaagaa tttttataat aaataaaatc aatatggaga
     1621 tatgttatgg gcacaacagg atttacaata attgacttaa ttatcttgat tgtttattta
     1681 cttgcggtgt tggttgcagg tatctatttc tctaaaaaag agatgaaagg aaaagagttc
     1741 tttaaaggag atggttcggt tccttggtat gttacttcgg tatccatttt tgccacaatg
     1801 ctcagtccga tttccttctt gggactcgct ggtaactctt atgcaggtag ctggatttta
     1861 tggtttgctc aattagggat ggttgtagct attccactga caattcgttt tatcttacct
     1921 atctttgcac ggatagacat cgatacggca tatgattact tggataaacg ttttaattct
     1981 aaagcacttc gtattatttc agcactcttg tttattattt atcaattggg acgtatgtct
     2041 atcattatgt acctcccatc agctggttta tcagtattga caggaattga catcaatatt
     2101 ttgattattt tgatgggtgc aattgcaatt gtttattctt atactggtgg tctaaaatcc
     2161 gtattatgga cagactttat tcaaggtgtg gttctgatta gtggtgtcgt tgtagcttta
     2221 tttgtactga tggctaatat taaaggtggc tttggtgcag tagcagaaac attagcaaac
     2281 gggaaattcc ttgctgcaaa tgaaaaactt ttcgatccta acttgctttc aaactccatc
     2341 ttcttaattg tgatgggttc aggctttaca atcttgtctt cctatgcttc atctcaagat
     2401 ttggttcaac gttttactac aacacaaaat attaagaaac ttaataaaat gttgttcaca
     2461 aatggtgtct tgtcacttgc aattgcaaca gtcttttact tgatcggtac aggcttgtac
     2521 gtattctatc aagtacaaaa tgcaggtagt gcagctagca atatccctca agaccaaatc
     2581 tttatgtact ttattgcata ccagttacca gtaggtgtca caggtttgat cttggcagcg
     2641 atttatgcag cagctcaatc aactgtttca acaggtttga actctgttgc aacttcatgg
     2701 acattggata ttcaagatgt catttctaaa aatatgtcag aggatcgtcg tacgaaaatt
     2761 gcacaattcg tatctctagc agtaggttta ttctcaattg ttgtttccat tatcatggct
     2821 cattcagata ttaaatctgc atacgaatgg ttcaatagtt tcatgggact tgtacttggt
     2881 ctacttggtg gtgtattcat tcttggtttt gtttctaaaa aagcaaataa acaaggtgct
     2941 tatgcagcgc tgattgtatc aaccatcgtt atggtattta ttaaatactt ccttcctcca
     3001 acagctgtta gctactggac atattcattg atttcaattt ctgtatcagt agtttcaggt
     3061 tatattgtat ctgttcttac tgaaaataaa gtatctgcac ctaaatatac aacgattcat
     3121 gatatttcag aaattaaagc ggattcaagt tgggaagttc gtcactagtc actaatagaa
     3181 aagagaaaat aatatgatta tttctgaaca atctgatttt agacgatatg cttctgtcaa
     3241 taaacatttt tcaaaagtct gtgatttttt agaaaataca aatttgacag atttagttga
     3301 tggaaaaatt gatattgacg gggaaaatgt ttttgca
//
LOCUS       DAVKGC010000137         3281 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKGC010000137 DAVKGC010000000
VERSION     DAVKGC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.137"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..463)
                     /locus_tag="VW049_001906"
     CDS             complement(<1..463)
                     /locus_tag="VW049_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432963.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFHSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCG"
     gene            complement(453..650)
                     /locus_tag="VW049_001907"
     CDS             complement(453..650)
                     /locus_tag="VW049_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432964.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(654..1448)
                     /locus_tag="VW049_001908"
     CDS             complement(654..1448)
                     /locus_tag="VW049_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432965.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLTKGQKVVNVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(1856..2281)
                     /locus_tag="VW049_001909"
     CDS             complement(1856..2281)
                     /locus_tag="VW049_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432966.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2415..2792)
                     /locus_tag="VW049_001910"
     CDS             complement(2415..2792)
                     /locus_tag="VW049_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432967.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKKKCY"
     gene            complement(2814..3020)
                     /locus_tag="VW049_001911"
     CDS             complement(2814..3020)
                     /locus_tag="VW049_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432968.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(3007..3168)
                     /locus_tag="VW049_001912"
     CDS             complement(3007..3168)
                     /locus_tag="VW049_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9432969.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
BASE COUNT         1056 a          658 c          408 g         1159 t
ORIGIN      
        1 gaccacaatt actcaatacc gaatacccct gaatggtttc tttttcatgc accaatatat
       61 tataataatc ttcatccaat aataaggctg ataaactagc atcttggtca aaatataagg
      121 gaatttctcg accgtccttc tttaatggat attctggtaa aatactaaat agttcaatca
      181 tagaatgaaa ctcaggatta gttgttgtaa atcgaaaaag ctgcgctttc tcatctttct
      241 gacttccttg atactctccc aattctaaaa agtgattcaa ggtagtataa aattccttat
      301 tttttacttc atcaatgatg accatatcat aatcttttgt tgtgcgactt ttaaatcctt
      361 gcgaatccaa tacgatagag gtagcagttc ccccaatcag aacataatag ttctgaaaat
      421 ccgcaaacat ttcttgaaaa ataactttcg tattagctgg catcatcttc tcccagatac
      481 tgtaatatca tattttctag tgcttcactc tcttcctcta tacgtgggtc atcatcatct
      541 tttaaggtca aataaagaga aatcggatct acaaattgtt tatcatgatt atttttaaaa
      601 tcattccaaa actcagatac aaaaggacga tatttccata tctctagcat ctttcatttt
      661 aaaacatgct gagaaagtgg caaggataac tgattgaatt ttctctgcca tatgacatag
      721 ctagtatttt catccgtttc agctaaaaaa gttgaatgcg acaaagcata agcaccacca
      781 tatagaaggt tagaaacaga ttttatctgc tttatatcgc catctggcaa taaaatccgt
      841 tttttgatgg gattaaataa acatgacacg gattttaaga ataattcttt ctttgacacc
      901 gtatatgtgt aaagcttatt ttgcttgttt aaccaatata aagctttaaa agtcctcaaa
      961 cacctataaa ttgttgagtt tggaagtcca gtgacttgtg aaagcaaatc aacatttact
     1021 actttttgac cttttgtcaa taaaaaggca atccacgtta attgttcgct aggtgttaat
     1081 tccttaggga tttcagtatc attcgcattg agtactagtc ccaatggagg gaagaagagg
     1141 tttcccttaa agtctacaaa cggaactcta gcttgaagta attgcttttt ttcactgtct
     1201 gataacttcg aaaacaccaa aacaacatcc atattggctt tttcacccat agtgcgagcc
     1261 tgagtaacaa atgaactcaa actccccctt ctcttttctt taatcaataa aaatgattcc
     1321 ttatgaaaag tcatttgagt atactcaaac ctttttacaa aactgattgg taaagtcaaa
     1381 gtcagttcct caatactgac atcaacataa aaggattgaa aaattgacat tatctttttg
     1441 acattcatgt tttttcctta ttctatcatt tttattatat tttatcattc ggtataataa
     1501 aatataagtt ttttgataaa ataaccaaaa actactcgct atcccctctc acattaccct
     1561 acatatcgtt tgacatgcga ctgatagttc aggaaaactt caaggagaac ttttctctca
     1621 tccactatgc aggacttact atttcacttc tactcctata ggctcaattt gacacttttc
     1681 tttttcgaat tgctcatatg cctcctgaat agtgagttcg atttatccaa atttattttt
     1741 gtctattttg atgaatactt ccacgaagat agtctgaaaa tgaatttgca ccaattttcc
     1801 ttctcttttt aagcatatta ttccagatat gattgccctc ttattcagta ataaattacg
     1861 ctttaaacgc ttcaatcagt atatctcctg ttgtcatatg ttctaaagaa aattcttcta
     1921 catatggttt aacatctctt aggttattac ttcctaatat cccattatgc tttttcgcta
     1981 agaaaataga agtcgcttct cccttaccaa taatcttgtt actatcatga tttcttgtta
     2041 aatctctata taatgcgtat tcttcagttc caatgtctat gctcacaatc tcagctgaac
     2101 ccttagttac caactggtct atcctagatt ttaaatgggg aattgtaggt atattgattt
     2161 catcatacac ctcttgtgga ataacaattt tacccaaata gagcttttct aaaagatgtt
     2221 cagtgccaac ccataaaaat actgaaatac aatctgcatc aataaataca cgactagtca
     2281 aacgacaact cccccctctt catctagccc atatacaata tcatatctga aagcatctag
     2341 taacagttcc tcatacttcc cttgcgaaat tctgttattt tctaaaagtt gttcagttag
     2401 attaatatat tatcctaata acatttcttt ttactttctg acaaaggacg atataaactt
     2461 gtatcatagc ctaatcttga agctgtctct ataacactaa tatccatatt tttaatttct
     2521 tctgcatcaa ggtatccatc attcctcaat ctatataaca tagctttatg actgatacca
     2581 taaaactgac ccaattttat aatatcttct acttcaagat gagttctatt ggcattttct
     2641 ctgatttcct caaccatcct atacagtgaa gatgggaaaa ttaaaaaata agaagcaaac
     2701 tgatccgctt ttctttcagt ttcatctcct tcaccaatca agataagact gactgaactc
     2761 ttcttcacct catcataata aagatgatac agttcatgtg ctaaagaaaa tctttacctt
     2821 cctaatgaca tgtctgaatt gactgcaatg agactgaact gagttccttt ataacagact
     2881 ccgctaatat tctttccgag tccataaaat accagcgtca aattttctat cttttgtacc
     2941 aatttaaaaa tatctatcgg cgattcacca tcagctccca acttttttct aagatttgga
     3001 gctttattac ttaaatccat tcgtgatatt cctttcacct ttttccattt tggcaatcat
     3061 gctttgattc aaagacaaat actcagcaat cttatcttga gtaagatttg aagatattct
     3121 aagttctttt attctttttc caacatcaca tacatttatc acatacattt atcatattca
     3181 tacacctctc ataaaaagaa tagcacactc ttgtcataat tttttaaata aaaaaattat
     3241 gacaaaacaa ggaagcaatt tattgatgct acttaaaaat c
//
LOCUS       DAVKGC010000138         3219 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKGC010000138 DAVKGC010000000
VERSION     DAVKGC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.138"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            210..1055
                     /locus_tag="VW049_001913"
     CDS             210..1055
                     /locus_tag="VW049_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9432970.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKNFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            1045..2148
                     /locus_tag="VW049_001914"
     CDS             1045..2148
                     /locus_tag="VW049_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9432971.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            2150..3136
                     /locus_tag="VW049_001915"
     CDS             2150..3136
                     /locus_tag="VW049_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9432972.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
BASE COUNT         1009 a          547 c          677 g          986 t
ORIGIN      
        1 caatgccttt ggttttcgaa actttgaaaa cttcaaaaaa cggattttta tcgctctgaa
       61 catcaaaaaa gaaaggacga aatttgtcct ttctcaagct tagcttttct tcaacccact
      121 acagttgaca aagagcctat tttttacagt ctgcaataga ttttcacccc ttgtttgtgg
      181 tataatgaaa cgatacgata gaaagaataa tgaacaatat gactgattta aaagcaattc
      241 aggctcgtag tctggagatg gctgaatatt ttgtggcctt ttgtaaagaa catgatttgc
      301 tctgttatct ctgtggcgga ggcgctattg gtgcccttcg aaacaaggga tttattcctt
      361 gggacgacga cctagacttt tttatgcctc gtaaagatta tgagaaatta gcagaattat
      421 ggcctcgtta tgcagatgaa cgttatttct tgtcaaagag tcacaagaat tttgttgatc
      481 gtaatctttt tattaccatt cgtgacaagg aaaccacctg tatcaagcct tatcagcagg
      541 atttggattt gccacatggt ctggccttgg atgttttgcc tttggattat tatccgaaaa
      601 atccagctga gcggaaaaaa caggttcgtt gggccttgat ttattcactc ttttgtgcgc
      661 aaactattcc agaaaagcat ggtgctctca tgaaatgggg aagtcgcatt ttactgggtt
      721 tgactccaaa atctctccgt tatcgcatct ggaaaaaagc tgagaaagaa atgactaagt
      781 atgatttggc tgattgtgat ggcattacag aattatgctc aggtcctggc tacatgagaa
      841 acaagtaccc aatcacatct tttgaagaca atcttttctt gccatttgaa ggaacagaga
      901 tgcctattcc aatcggctat gatgtctatc tcagaactgc ttttggggat tatatgacgc
      961 ctccaccagc agacaagcag gtaccgcatc atgatgctgt catcgctgat atggataagt
     1021 cttatacaga atacaaggga gaatatggtg gctaagaaaa aaatcttatt ttttatgtgg
     1081 tctttttctc ttggaggtgg tgcagagaag attctatcaa ccattgtttc aaatctggat
     1141 ccagaaaagt atgatattga tattcttgaa atggagcact ttgacaaggg atatgaatct
     1201 gttccaaagc atgtacgcat tttaaaatcc cttcaagatt atcgccaaac cagatggtta
     1261 cgagcttttt tgtggagaat gagaatttat tttccaagac tggctcgtcg tttgcttgta
     1321 aaggatgatt atgatgttga agtttctttt accattatga atccaccact gttgttctct
     1381 aaaagaagag aagtcaagaa gatatcttgg attcatggaa gtattgaaga acttcttaag
     1441 gatagctcta aaagagaatc acatagaagc cagttggatg ctgcgaatac aattgtaggg
     1501 atttcaaaaa agaccagcaa ttctatcaag gaagtttatc cagattatac ttctaaatta
     1561 cagacaatct acaatggata tgattttcag actattctag aaaaatctca agagaagatc
     1621 gatatcgaga ttgctcctca aagtatctgt actatcggac ggattgagga aaataagggt
     1681 tctgaccgtg tagtggaagt gatacgatta ttacaccaag agggaaaaaa ctatcatctc
     1741 tattttatcg gggctggtga tatggaagag gaactgaaaa aacgagtcaa agagtatggg
     1801 attgaggact atgtacattt ccttggttat caaaaaaatc cttatcagta tctatctcag
     1861 acgaaagttc ttttgtctat gtctaaacaa gaaggttttc ctggagtgta tgtggaggcc
     1921 ttgagtctgg gactcccttt tatctctacg gacgttggag gggctgagga attatcccaa
     1981 gaaggacgat ttggacaaat cattgagagc aatcaagagg cagctcaggc gattactaat
     2041 tacatgactt ctgcctcaaa ctttgatgtc gatgaggcta gccaattcat tcaacaattt
     2101 acaattacaa aacaaatcga acaagtagaa aaactattag aggagtagca tggaaactgc
     2161 attaattagt gtgattgtgc cagtctataa tgtggcgcag tacctagaaa aatcgatagc
     2221 ttccattcag aagcagacct atcaaaatct ggaaattatt cttgttgatg atggtgcaac
     2281 agatgaaagt ggtcgcttgt gtgattcaat cgctgaacaa gatgacaggg tgtcagtgct
     2341 tcataaaaag aacgaaggat tgtcgcaagc acgaaatgat gggatgaagc aggctcacgg
     2401 ggattatctg atttttattg actcagatga ttatatccat ccagaaatga ttcagagctt
     2461 atatgagcaa ttagttcaag aagatgcgga tgtttcgagc tgtggtgtca tgaatgtcta
     2521 tgctaatgat gaaagcccac agtcagccaa tcaggatgac tattttgtct gtgattctca
     2581 aacatttcta aaggaatacc tcataggtga aaaaatacct gggacgattt gcaataagct
     2641 aatcaagaga cagattgcaa ctgccctatc ctttcctaag gggttgattt acgaagatgc
     2701 ctattaccat tttgatttaa tcaagttggc caagaagtat gtggttaata ctaaacccta
     2761 ttattactat ttccatagag gggatagtat tacgaccaaa ccctatgcag agaaggattt
     2821 agcctatatt gatatctacc aaaagtttta taatgaagtt gtgaaaaact atcctgactt
     2881 gaaagaggtc gcttttttca gattggccta tgcccacttc tttattctgg ataagatgtt
     2941 gctagatgat cagtataaac agtttgaagc ctattctcag attcatcgtt ttttaaaagg
     3001 ccatgccttt gctatttcta ggaatccaat tttccgtaag gggagaagaa ttagtgcttt
     3061 ggccctattc ataaatattt ccttatatcg attcttatta ctgaaaaata ttgaaaaatc
     3121 taaaaaatta cattagaacg ggggtgacca agtgattgat gggaaacgat tattatttag
     3181 tttgaccata gtcagttatg ccttgacgct agtaagtgg
//
LOCUS       DAVKGC010000139         3160 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKGC010000139 DAVKGC010000000
VERSION     DAVKGC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.139"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..3085
                     /gene="phtE"
                     /locus_tag="VW049_001916"
     CDS             <1..3085
                     /gene="phtE"
                     /locus_tag="VW049_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9432973.1"
                     /translation="AVIVSLSLCAYALNQHRSQENKDNNRVSYVDGSQSSQKSENLTP
                     DQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSEELLMKDPNYQLK
                     DADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEHVKDNEKVNSNVA
                     VARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLSASELAAAKAHLA
                     GKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELYDSPSAQRYSESD
                     GLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPISGTGSTVSTNAK
                     PNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAYIVRHGDHFHYIP
                     KSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRIIAEDESGFVMSHG
                     DHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSNAKEMKDLDKKIE
                     EKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVGIGHSHSNYELFK
                     PEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVSFSFPPELEKKLG
                     INMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFKYTIASKDYPEVS
                     YDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDALVRVFDEFHGNAY
                     LENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYIVEVPILEKENQT
                     DKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTSNSTLEEVPTVDP
                     VQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTGEAPQGNGENKPS
                     ENGKVSTGTVENQPTENKPADSLPEAPNKKPVKPENSTDNGMLNPEGNVGSDPMLDPA
                     LEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVIKKNLSDLIA"
BASE COUNT         1170 a          531 c          620 g          839 t
ORIGIN      
        1 agctgttatc gtatccttga gtctatgtgc ctatgcacta aaccagcatc gttcgcagga
       61 aaataaggac aataatcgtg tctcttatgt ggatggcagc cagtcaagtc agaaaagtga
      121 aaacttgaca ccagaccagg ttagccagaa agaaggaatt caggctgagc aaattgtaat
      181 caaaattaca gatcagggct atgtaacgtc acacggtgac cactatcatt actataatgg
      241 gaaagttcct tatgatgccc tctttagtga agaactcttg atgaaggatc caaactatca
      301 acttaaagac gctgatattg tcaatgaagt caagggtggt tatatcatca aggtcgatgg
      361 aaaatattat gtctacctga aagatgcagc tcatgctgat aatgttcgaa ctaaagatga
      421 aatcaatcgt caaaaacaag aacatgtcaa agataatgag aaggttaact ctaatgttgc
      481 tgtagcaagg tctcagggac gatatacgac aaatgatggt tatgtcttta atccagctga
      541 tattatcgaa gatacgggta atgcttatat cgttcctcat ggaggtcact atcactacat
      601 tcccaaaagc gatttatctg ctagtgaatt agcagcagct aaagcacatc tggctggaaa
      661 aaatatgcaa ccgagtcagt taagctattc ttcaacagct agtgacaata acacgcaatc
      721 tgtagcaaaa ggatcaacta gcaagccagc aaataaatct gaaaatctcc agagtctttt
      781 gaaggaactc tatgattcac ctagcgccca acgttacagt gaatcagatg gcctggtctt
      841 tgaccctgct aagattatca gtcgtacacc aaatggagtt gcgattccgc atggcgacca
      901 ttaccacttt attccttaca gcaagctttc tgccttagaa gaaaagattg ccagaatggt
      961 gcctatcagt ggaactggtt ctacagtttc tacaaatgca aaacctaatg aagtagtgtc
     1021 tagtctaggc agtctttcaa gcaatccttc ttctttaacg acaagtaagg agctctcttc
     1081 agcatctgat ggttatattt ttaatccaaa agatatcgtt gaagaaacgg ctacagctta
     1141 tattgtaaga catggtgatc atttccatta cattccaaaa tcaaatcaaa ttgggcaacc
     1201 gactcttcca aacaatagtc tagcaacacc ttctccatct cttccaatca atccaggaac
     1261 ttcacatgag aaacatgaag aagatggata cggatttgat gctaatcgta ttatcgctga
     1321 agatgaatca ggttttgtca tgagtcacgg agaccacaat cattatttct tcaagaagga
     1381 cttgacagaa gagcaaatta aggctgcgca aaaacattta gaggaagtta aaactagtca
     1441 taatggatta gattctttgt catctcatga acaggattat ccaagtaatg ccaaagaaat
     1501 gaaagattta gataaaaaaa tcgaagaaaa aattgctggc attatgaaac aatatggtgt
     1561 caaacgtgaa agtattgtcg tgaataaaga aaaaaatgcg attatttatc cgcatggaga
     1621 tcaccatcat gcagatccga ttgatgaaca taaaccggtt ggaattggtc attctcacag
     1681 taactatgaa ctgtttaaac ccgaagaagg agttgctaaa aaagaaggga ataaagttta
     1741 tactggagaa gaattaacga atgttgttaa tttgttaaaa aatagtacgt ttaataatca
     1801 aaactttact ctagccaatg gtcaaaaacg cgtttctttt agttttccgc ctgaattgga
     1861 gaaaaaatta ggtatcaata tgctagtaaa attaataaca ccagatggaa aagtattgga
     1921 gaaagtatct ggtaaagtat ttggagaagg agtagggaat attgcaaact ttgaattaga
     1981 tcaaccttat ttaccaggac aaacatttaa gtatactatc gcttcaaaag attatccaga
     2041 agtaagttat gatggtacat ttacagttcc aacctcttta gcttacaaaa tggccagtca
     2101 aacgattttc tatcctttcc atgcagggga tacttattta agagtgaacc ctcaatttgc
     2161 agtgcctaaa ggaactgatg ctttagtcag agtgtttgat gaatttcatg gaaatgctta
     2221 tttagaaaat aactataaag ttggtgaaat taaattaccg attccgaaat taaaccaagg
     2281 aacaaccaga acggccggaa ataaaattcc tgtaaccttc atggcaaatg cttatttgga
     2341 caatcaatcg acttatattg tggaagtacc tatcttggaa aaagaaaatc aaactgataa
     2401 accaagtatt ctaccacaat ttaaaaggaa taaagcacaa gaaaactcaa aacttgatga
     2461 aaaggtagaa gaaccaaaga ctagtgagaa ggtagaaaaa gaaaaacttt ctgaaactgg
     2521 gaatagtact agtaattcaa cgttagaaga agttcctaca gtggatcctg tacaagaaaa
     2581 agtagcaaaa tttgctgaaa gttatgggat gaagctagaa aatgtcttgt ttaatatgga
     2641 cggaacaatt gaattatatt taccatcggg agaagtcatt aaaaagaata tggcagattt
     2701 tacaggagaa gcacctcaag gaaatggtga aaataaacca tctgaaaatg gaaaagtatc
     2761 tactggaaca gttgagaacc aaccaacaga aaataaacca gcagattctt taccagaggc
     2821 accaaacaaa aaacctgtaa aaccagaaaa ctcaacggat aatggaatgt tgaatccaga
     2881 agggaatgtg gggagtgacc ctatgttaga tccagcatta gaggaagctc cagcagtaga
     2941 tcctgtacaa gaaaaattag aaaaatttac agctagttac ggattaggtt tagatagtgt
     3001 tatattcaat atggatggaa cgattgaatt aagattgcca agtggagaag tgataaaaaa
     3061 gaatttatct gatctcatag cgtaaggaat agcagtagaa aaagtctgaa tcaaaaatga
     3121 agttctctca aaagttagaa ataaaactct gactttggga
//
LOCUS       DAVKGC010000140         3143 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKGC010000140 DAVKGC010000000
VERSION     DAVKGC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.140"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..598)
                     /locus_tag="VW049_001917"
     CDS             complement(<1..598)
                     /locus_tag="VW049_001917"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEU9432974.1"
                     /translation="MAISQMKRISLLFTKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFSTIQISHTSNSHHGVIEGNDALNYLMNKQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERNIQSNNEEIERLIKW
                     EKLEIIPAHLEQFSFCRGKVGTIPRTVDNRLYNNFLENN"
     gene            complement(588..911)
                     /locus_tag="VW049_001918"
     CDS             complement(588..911)
                     /locus_tag="VW049_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432975.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELISIESNLEMKQQNLIDEVNKTIKENDAKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            complement(936..1826)
                     /locus_tag="VW049_001919"
     CDS             complement(936..1826)
                     /locus_tag="VW049_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9432976.1"
                     /translation="MKIIGIDIGGTTIKADLYDDFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYVLNYSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTSEIEKRF
                     GLSTFVENDVNCAALGELWEGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLSSGDKVAKETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNVVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            complement(1983..3092)
                     /locus_tag="VW049_001920"
     CDS             complement(1983..3092)
                     /locus_tag="VW049_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9432977.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
BASE COUNT          935 a          653 c          417 g         1138 t
ORIGIN      
        1 tattgttttc taaaaaatta ttgtataagc gattatctac agtccttgga attgttccga
       61 cttttcctct acagaaagaa aattgttcta aatgcgcagg aataatttct aatttttccc
      121 actttattaa tctctctatt tcctcattat tactttgaat gtttctttct aattcatgaa
      181 ctctgtttat ttgatgattc acttttttaa gaacaccctc agcaacatta gctttaccaa
      241 atttttctaa ttcttcataa gaggttgtta tcggaggttg ctgtaataac ttaaacgtgt
      301 tttctttcgg taggtattct tgtaatttct ctacagttgc ttccaaatgt tgttgtttat
      361 tcatcaaata attcaaggca tcatttcctt caataacccc atgatgggaa ttagaagtgt
      421 gagaaatttg aatagttgaa aatacaacct catctttttc tagcgcttct gaccagttat
      481 cctgaacctt taaatcacgg aactgcactg actctagttc ttgaatagtt tttaaaacat
      541 catcaagact actcttagta aatagtagag aaattctttt catctgacta atagccatat
      601 ttttctacca ccctaccaat aattttttct acaaattctc ctctcctact tgatgaaata
      661 tactgaatct ttgcatcatt ttccttgatt gttttattaa cctcatcaat gaggttctgt
      721 tgtttcattt ctaaattgct ttctattgaa attaattctt cgtgactttt ttcatcataa
      781 cttttttcta gtttttccaa ttcttcttgg gagacagctt tatacgtata aatctcatta
      841 tcatatgatt ttataatatc ggttgcaaca ttctcaatat cttgcattgt cgaaaataca
      901 gagttagtca ttgtgacacc tccttatctt tcacactatt tattagaaat tctatctaag
      961 aaatttttta cagctcctat acgaccagct tcatttccta atgtagctgc cacaacattt
     1021 ttaggcaaaa acctattatc cattgcattt ttagctaaag aactttgaat ttcaggtaac
     1081 aaaatatcct ttttagccag aatcccacct cctaatatga gaatttctgg attaagtaga
     1141 taagaaatag ttaataaacc ttttgttaga ttttctacaa aaatttcaaa agtttcttta
     1201 gcgactttat ctccagaact taaatcagta aagaaagtac gtccagtttg attagttttc
     1261 aagctcttct tttgatataa atgaatcaat gcggttgttg aggctttgct ttgccaatcc
     1321 gaattaccta caggaatata gcctacttca ccagcagtat agttaaatcc gttaacaatt
     1381 tgtccattga caataatact gcctcctata cctgttccaa tagtaaccat tactacattt
     1441 ttcttatctt tggcttgtcc ctcccacaat tcacctaatg cagcacaatt aacatcattt
     1501 tcaacaaaag tagacaaccc aaaacgtttt tctatttcgg aagtaaagtt tactccgata
     1561 taccctggta ttgtatagcc tgcatagatg atttctcctg tattagcatt aacaactcca
     1621 gcagtggaaa tcccaacacc atcaattgaa taatttaaaa catactcacc aattaaatca
     1681 cagacctgat ttaatatctg gttcgttccc aaatcatagt caataattgt ttctatctct
     1741 ttgaactgat ttaaactcgt tccaaaatca tcgtataaat ctgccttaat tgttgttccg
     1801 ccaatatcga tccctataat cttcatttca tcactcccac atttttgatc tcattacttt
     1861 ttgataaaaa atgggactgg gcattatccc agtcccatct ctatttcttt attcaatgag
     1921 ttgtatcaca gaactttaga aattatataa tgacaagaat ttactattct gttttacaat
     1981 agttaagcat ttgtgatttc tgaaagactt acttttcgat cttctttaac tgataaagta
     2041 catgcatcag cggtagcgat tgattctaaa gcagctacac cattgagaag tttttcatat
     2101 tcttctgtaa tttctccacc tttaatgatg tcatgtagat attccatttc tttatcaata
     2161 catgtttgta accataatgg gcaacgtact cctggtttac cgtacgcaat tgctccatcc
     2221 ataccacgac cggtatagat agctgtacgg tcatcatctt cctcttgagt ttcatgtact
     2281 aagaagtgtg attctccttc acctttaaca cgaagagtac ctccagtatt gaacaagtca
     2341 agtttgatag ctccttcagt tccttgaatc aagacgtagt gttcacccca acggaaagca
     2401 ttaccatatt ccaaaacagc ataacgatca tcagagtatt ctaggtttac aatgagcata
     2461 tcatcttcat caccaaagtt ttcaccttta tgatatacat tgcctcctac cattgtcgct
     2521 ttttcaggaa gtcctcccat gatgaactga atgcaatcta attcatgaat atggtggtac
     2581 aaatgtcctc cagattgaga acgaagtttc ttccatgata cagttggttg ttgttcttcc
     2641 caacctgtac gagcggcatg gcaataaaga actttaccga ttttaccttg agtaatcaat
     2701 tctttagcat ggtgtacacc attaaagaag ttcatgatgt gaccagccat aaagataaca
     2761 ttattttctt tacatgcgtc aaccatggct ttacaatctt cataagacaa tgcaattggc
     2821 ttttcacaaa atacgtgttt gccatgttga gcagctttca caacaggttc acggtgaagg
     2881 tagctaggtg aagctacgat cacacaatca atatcttcac gtgctacaag ttcatctaaa
     2941 cttgcacaaa catctgatcc caactcttga gcaacttctt ctccatgatt tgggtcaaat
     3001 accgcaacca cttttgcatc ttcaattttg ttcattgagc gagccaaatc agctccaaaa
     3061 tatccagctc caacaatacc gtaatttacc ataattttta tcctctttct atatattttt
     3121 aaatttcagc tttaattctt ttt
//
LOCUS       DAVKGC010000141         3137 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKGC010000141 DAVKGC010000000
VERSION     DAVKGC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.141"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            78..1589
                     /locus_tag="VW049_001921"
     CDS             78..1589
                     /locus_tag="VW049_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9432978.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            1611..2942
                     /locus_tag="VW049_001922"
     CDS             1611..2942
                     /locus_tag="VW049_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9432979.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT          855 a          658 c          679 g          945 t
ORIGIN      
        1 gaaagcgttt tcttaaatgt ttatttaaga aagtagttgg ttttttacac tttgttagac
       61 atcaggagga aaaacaaatg agtgaaaaag ctaaaaaagg gtttaagatg ccttcatctt
      121 acaccgtatt attgataatc attgctatta tggcagtgct aacttggttt atccctgcgg
      181 gggcctttat agaaggtatt tacgagactc agcctcaaaa tccacaaggg atttgggatg
      241 tcctcatggc accgattcgg gctatgctag gtactcatcc agaggaaggt tcgctcatta
      301 aagaaacgag cgcagcgatt gatgtagcct tcttcatcct tatggttggt ggtttccttg
      361 gcattgtcaa caaaactggt gctcttgacg tagggattgc ctctatcgtg aagaagtata
      421 agggccgcga aaaaatgtta attttggtac tgatgccttt gtttgccctc ggtggtacaa
      481 cttatggtat gggtgaagaa acaatggctt tctatccact ccttgtgcca gttatgatgg
      541 ccgttggttt tgatagcctg actggtgttg caattatttt gctcggttct caaatcggct
      601 gtttggcatc tactctgaat ccatttgcga caggtattgc ttcagcgact gcgggagttg
      661 gtacagggga cggtatcgta cttcgtctga tcttctgggt taccttgact gctcttagta
      721 cttggtttgt ttaccgttat gcggataaga ttcaaaaaga tccgactaag tcactggttt
      781 atagtactcg caaagaagat ttgaaacact ttaacgtaga agaatcttca tctgtagaat
      841 ctacacttag cagcaaacaa aaatcagttc tcttcttatt tgtgttgaca ttcatcttga
      901 tggtattgag cttcattcca tggacagacc ttggcgttac catttttgat gactttaata
      961 cttggttgac tggtcttcca gttattggta atattgtcgg ttcatctact tctgcactag
     1021 gtacttggta cttcccagaa ggcgcaatgc tctttgcctt tatgggtatc ctgattggtg
     1081 ttatttatgg tcttaaagaa gataagatta tctcttcctt catgaatggt gctgctgact
     1141 tgctcagtgt tgccttgatc gtagcgattg ctcgtggtat tcaagttatc atgaacgacg
     1201 gtatgattac cgatacaatc ctcaactggg gtaaagaagg cttgagcggt ctatcttcac
     1261 aagtctttat cgttgtaact tatatcttct atctacctat gtcattcttg atcccatctt
     1321 catctggtct tgccagcgca actatgggta tcatggctcc acttggagaa tttgtaaatg
     1381 tccgtcctag cttgattatc actgcttacc aatctgcttc aggtgtcttg aacttgattg
     1441 caccaacatc tggtattgtg atgggagctc ttgcacttgg acgtatcaac attggtactt
     1501 ggtggaaatt catgggcaaa ctcgtagtcg ctattattgt agtgaccatc gcccttcttc
     1561 tccttggaac cttccttcca ttcctataaa atagtgagtg aggtgattcc atgaaaatag
     1621 atataacaaa tcaagttaaa gatgaatttc ttatatcatt aaaaaccttg atttcctatc
     1681 cttcagtact caatgaagga gaaaatggaa caccttttgg acaagcaatc caagatgtcc
     1741 tagaaaaaac tttagagatt tgtcgagaca taggtttcac tacctatctt gaccctaaag
     1801 gttattacgg atatgcagaa atcggtcagg gagcagagct tctggccatt ctctgtcatt
     1861 tggatgttgt tccatcaggt gatgaagcag attggcagac accgccattt gaagcaacta
     1921 tcaaagacgg ctgggtattc ggacgtggtg tccaagatga taaaggccct tcgctcgcag
     1981 ctctctatgc agtaaaaagc ttgctggacc aaggtattca gttcaaaaag cgcgtacgct
     2041 ttatctttgg taccgatgag gaaaccctct ggcgctgcat ggcacgctac aataccatcg
     2101 aagaacaggc cagtatgggc tttgcacctg actcatcttt tcctctgacc tatgctgaaa
     2161 aagggcttct acaggtcaaa cttcatggcc ctggatcgga tcaactagag cttgaagtag
     2221 gaggcgcctt taacgttgta ccagacaagg ccaactacca aggtcccctc tatgaacagg
     2281 tttgtaacgg tctcaaagaa gctggttatg attaccaatc cactgaacaa accgtaacgg
     2341 ttctcggagt gccaaagcat gctaaggatg ctagtcaagg tatcaatgct gtcatccgac
     2401 tagctaccat tcttgctcct ctccaagaac accctgctct cagttttctt gcaacacaag
     2461 caggtcaaga cggcacagga agacaaatct ttggtgatat agcagatgaa ccttctggtc
     2521 acctatcctt taatgtcgca ggtctcatga tcaatcatga acgttctgaa atccgtattg
     2581 acattcggac tcctgtctta gctgacaagg aagaactagt agagttgctt acaagatgtg
     2641 cacaaaacta ccaactccgc tacgaagagt ttgactatct agcgcctcta tacgtcgcaa
     2701 aagacagtaa actcgttagc acactgatgc aaatctacca agaaaagact ggcgataaca
     2761 gtcctgctat ttcatccggt ggtgccactt ttgctcgcac catgccaaat tgtgtagcct
     2821 tcggcgcctt attcccagga gcgaagcaaa cagaacatca ggcaaatgaa tgtgccgttc
     2881 tagaagattt gtaccgtgct atggatattt atgccgaagc cgtctatcga cttgcaactt
     2941 aatcaggcaa ctgtttctac caaaaaaaat cgaccgatta atgaactgca ccccaaaagt
     3001 tagacagaat aaatctaact tttggggtgt tttattatga aattgagtta tgaagataaa
     3061 gttcagatct atgaactaag aaagcaagga caaagcttca aacagctttc aaaaagattt
     3121 ggtgtggatg tttctgg
//
LOCUS       DAVKGC010000142         3118 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKGC010000142 DAVKGC010000000
VERSION     DAVKGC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.142"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            288..2789
                     /locus_tag="VW049_001923"
     CDS             288..2789
                     /locus_tag="VW049_001923"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9432980.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLDVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
BASE COUNT          952 a          615 c          691 g          860 t
ORIGIN      
        1 ttcttcataa atcaaaaagc gacataggtt gtcggctatt tattgtgaat acattaatta
       61 gcattccagt tttatcttcg gtctaaaata agtattttgt gctatacgag ataagcttct
      121 tgacttactc cttgatttac tgcataacaa tgggataaaa agtgggagat agagcaattc
      181 atagtcatca aaattaatga gatacagtat acagtttttc ctttaaacac atttcaaatt
      241 ccctcaaaaa tggtataata gtaacatcac aaaattggag agagaccatg agtttttaca
      301 atcataaaga aattgagcct aagtggcagg gctactgggc agaacatcat acatttaaga
      361 caggaacaga tgcatcaaaa cctaagtttt atgcgcttga tatgttccct tatccgtctg
      421 gagctggtct gcacgtagga cacccagaag gttatactgc aaccgatatc ctcagtcgtt
      481 acaaacgtgc gcaaggctac aatgtccttc acccaatggg ttgggatgct tttggtttgc
      541 ctgcagagca atacgctatg gatactggta atgacccagc agaatttaca gcggaaaaca
      601 ttgccaactt caaacgtcaa attaatgcgc ttggattttc ttatgactgg gatcgtgaag
      661 tcaacacaac agatccaaac tactacaagt ggactcaatg gattttcacc aagctttacg
      721 aaaaaggctt ggcctatgaa gctgaagtgc cagtaaactg ggttgaggaa ttgggaactg
      781 ccattgccaa tgaagaagtg cttcctgacg gaacttctga gcgtggaggc tatccagttg
      841 tccgcaaacc aatgcgccaa tggatgctca aaatcacggc ttacgcagag cgcttgctca
      901 atgacttaga tgaactagat tggtcagagt ctatcaagga tatgcaacgc aactggattg
      961 gtaaatcaac tggtgccaat gtaactttca aagtaaaagg aacagacaag gaatttacag
     1021 tctttactac tcgtccggac acacttttcg gtgcgacttt cactgtcttg gctcctgaac
     1081 atgaattagt agacgctatc acaagttcag agcaagcaga agctgtagca gactataaac
     1141 accaagccag ccttaagtct gacttggctc gtacagacct tgctaaagaa aaaacagggg
     1201 tttggactgg tgcttatgcc atcaaccctg tcaatggtaa ggaaatgcca atctggattg
     1261 cagactatgt ccttgctagt tatggaacag gtgcggttat ggctgtgcct gcccacgacc
     1321 aacgtgactg ggaatttgcc aaacaatttg accttccaat cgtcgaagta cttgaaggtg
     1381 gaaatgtcga agaagctgcc tacacagagg atggcctgca tgtcaattca gacttcctag
     1441 atggactcaa caaagaagac gctattgcca agattgtggc ttggttggaa gaaaaaggct
     1501 gtggtcagga gaaggttacc taccgtctcc gcgactggct ctttagccgt caacgttact
     1561 ggggtgagcc aattccaatc attcattggg aagatggaac ttcaacagct gttcctgaaa
     1621 ctgaattgcc gcttgtcttg cctgtaacca aggatatccg tccttcaggt actggtgaaa
     1681 gtccactagc taacttgaca gattggcttg aagtgactcg tgaagatggt gtcaaaggtc
     1741 gtcgtgaaac caacactatg ccacaatggg ctggttcaag ctggtactac ctccgctata
     1801 ttgatccaca caatactgag aagttggctg atgaggacct cctcaaacaa tggttgccag
     1861 tagatatcta cgtgggtggt gcagagcatg ctgttcttca cttgctttac gctcgtttct
     1921 ggcacaaatt cctctatgac ctcgatgttg ttccgactaa ggaaccattc caaaaactct
     1981 ttaaccaagg gatgattttg ggaacaagct accgtgacca ccgtggtgct cttgtggcaa
     2041 ccgacaaggt tgaaaaacgt gatggttcct tcttccatgt agaaacaggg gaagagttgg
     2101 agcaagcgcc agccaagatg tctaaatcgc tcaagaacgt tgttaaccca gacgatgtgg
     2161 tggaacaata cggtgccgat acccttcgtg tttatgaaat gtttatggga ccactcgatg
     2221 cttcgattgc ttggtcagaa gaaggtttgg aaggaagccg taagttcctt gaccgagttt
     2281 accgtttgat tacaagtaaa gaaatccttg cggaaaacaa tggtgctctt gacaaggttt
     2341 acaacgaaac agtcaaagct gttactgagc aaattgagtc tctcaaattc aacacagcta
     2401 ttgcccaact tatggtcttt gtcaatgctg ctaacaagga agataagctt tatgttgact
     2461 atgccaaagg ctttattcaa ttgattgcac catttgcacc tcacttggca gaagaactct
     2521 ggcaaacagt cgcagaaaca ggtgagtcaa tctcttatgt agcttggcca acttgggacg
     2581 aaagcaaatt ggttgaagat gaaattgaaa ttgtcgtcca aatcaaagga aaagttcgtg
     2641 ccaaactcat ggttgctaaa gatctatcac gtgaagaatt acaagaaatc gctttagctg
     2701 atgaaaaagt caaagcagaa attgacggta aggaaatcgt gaaagtaatt gcggtaccga
     2761 ataaactcgt taatatcgtc gttaaataac gagtttatta gctctatctg ccaccttcaa
     2821 tagtccactg gactattgaa gccaactaaa ttagttaaca ttgttgtgaa ataaaaagaa
     2881 gtaactctag agtatgaact ttagagttac ttttagaata ggtcagaatg aatagattag
     2941 tgaacgatag caggaagtgt gaaattttat tatttaagat ttctattatt gtagtgtcag
     3001 ttatagtatt agtaatgtca atagtgggtg catattattt taaaaattgt ctttgggaca
     3061 aggaaagttt aaatttgaca ctcactttag acactctctt tgcagttttt attacttt
//
LOCUS       DAVKGC010000143         3108 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKGC010000143 DAVKGC010000000
VERSION     DAVKGC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.143"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     regulatory      complement(95..183)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(392..1021)
                     /locus_tag="VW049_001924"
     CDS             complement(392..1021)
                     /locus_tag="VW049_001924"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9432981.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALRGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGVKLMRKLL
                     PQMPLVAIGGIQTKHIKDIMKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(1023..1805)
                     /gene="thiM"
                     /locus_tag="VW049_001925"
     CDS             complement(1023..1805)
                     /gene="thiM"
                     /locus_tag="VW049_001925"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9432982.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPVVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            1889..1981
                     /locus_tag="VW049_001926"
                     /pseudo
     CDS             1889..1981
                     /locus_tag="VW049_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(2131..2799)
                     /locus_tag="VW049_001927"
     CDS             complement(2131..2799)
                     /locus_tag="VW049_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9432983.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYWAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(3014..3102)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          960 a          678 c          442 g         1028 t
ORIGIN      
        1 catcttcatc cagtttcgta aaagaaaaaa ctctaattac agatacgaat tagagttcag
       61 cttacaagat tagacagttc ttttcgacat acgaaaaaaa catttcacat ttcccttcgc
      121 cagtcttaac tgtatcaggt tcaatgggta tcatctcagc ctaaagcacc ccaaatgcct
      181 ttattattta attatgtgat tattataaca cacattttat actagttcaa gaaattgaac
      241 tggaaataca gccttgcact cacaaagaca gcagatcttt cttttgcaaa aaacaaatga
      301 cctgtttgat gaattagcta ttcaagctga atctagacat agctttttaa aaaaggaaaa
      361 tcctacttac ttagaatcca aggatagata tctattgttc actcatttcc cgaacagttt
      421 tttctatatt ttttgcatac gatattgccg aaatgattga aacgccgtca acattggtct
      481 tcataatgtc tttaatatgt ttcgtctgta tcccaccaat tgcaactaag ggcatttgtg
      541 gcaatagttt tctcatcaat ttaacacctt cataacctat agcaccacca gcatcatcct
      601 ttgactgggt atcaaataca ggaccaacac caacataatc tacatattca acttttgatt
      661 gttgaaattc ttcctcgttt tttatagaaa gaccaattat tttatctggc atcaattttc
      721 taatttcatc aacaccaata tcatcttgac ctacatgtac gccatcagcg tcaatttcca
      781 ttgccaaatc gatatcatca ttaacgataa acggcacatt gtatttttta caaagttctt
      841 taatttggat agctagctca agtttttcta agccttctaa agcaccctca cctttttctc
      901 gaaattgaaa taaggttata ccacctctta aggcttcctc aacgactgta tatagatttt
      961 ttccttggca agtagtcgtt ccacaaataa aatatagttt tagtaattct ttatgaaaca
     1021 tcttacttca ctcttttgaa ttcctttaca tcttcatctg taatctcgta taaggcattt
     1081 ataaattcaa ctttaaatgt cccaggaaga tgtccatttg gacgtttttc tgccatttct
     1141 ccagcgatat tgtaaaccaa cattgctgtt tctaatgatt tcaattcttg acctttttct
     1201 agtccgataa agcttgctac cactgcccct aataaacatc cagttccaat aacttttggc
     1261 atcatggcac taccattatg aatcgttacc acttctccat ttaccgcaat agcatccact
     1321 tcacctgtta ctactattgg aatattgaac ttctcatttg ctgctagagc aatctcgtca
     1381 atattatcta ctcccgcact atctactcct ttagatgcca catctattcc tactaaagag
     1441 gcaatctctc cagcattccc cctaatcgca gctagtttgt aattgttgat taaatcatct
     1501 gctacttttt ttctatattc tcctgctcca caggctacag gatctaaaac tactgggaca
     1561 ttatatttct ctgcaatttt cagagcagct tggtataatt tccaattttc atctgtcaat
     1621 gttcctatgt ttattaataa accaccagca tactttaaca aatcctctaa atctgctgga
     1681 aactcactca tggctggtga ggcgcccagt gctactaatc catttgctgt gaaatttttt
     1741 actacatcat tggtgataca aatgaccaat ggtgcttttt cttttaataa ttttaaactt
     1801 gtcatattga aatccttcct tttcacttta tacgatctac tgatttcgat ttatttctat
     1861 ctttcgttaa gaattttttt catttacatt gaatgattta tactcaatga aaatcaaaga
     1921 gcaaactagg aagctaaccg cagattgctc aaaacactgt tttgaggttg tggatagaac
     1981 tgacgaagtc agctcaaaac actgttttga ggttgtggat agaactgacg aagtcagtaa
     2041 ccatacctac ggcaaggtga aactgacgtg gtttgaagag attttcgaag agtcttacct
     2101 catcaaattt gtaaatatca tgagccttct ctagacatcg taaccaatat caaaaaaagc
     2161 taattctaaa gcgactgctt gattccatcg ttgctgaagt tctgtcaaat cttctcgatt
     2221 tttaccgaca cgattgagtt cgtcaaccag aaattgaacc cactctgcaa agaaaggacc
     2281 tctgtggaga ttgatccatt ccgaatgaat atagacttca ggtagagcca aatctttaga
     2341 accccagtct aaatagagac cttctgcaat gaccagcata accaaaagat gggcatagtc
     2401 tgatgaagcc actgcccaat acattagatc ctgaaaggct tttgttacag ggtgcaaagt
     2461 cacttctaga tagtcattct ctgctacttt taactcttta aaagcctttt ggaaataacc
     2521 atcttcatct gcttcaagaa agcctagttg cttggcaaaa cgaagcttgg attcaagttt
     2581 atctgcgtga gctacgcagg cacccagcat ggataagaag gcatcaaaga agtgataatc
     2641 ttgaatcaga tagtccttta agaccttatt ctcaattgtc cccgcaaaaa gttccttaac
     2701 aaaacgatga ttgattgcag cctgccaatc cttctgactg ctttttaata attctccaac
     2761 agtcaaacct ggctgaaatg catagtcttg tgtttccata tttacttctc ctctctttac
     2821 ttgttagtaa ttaataaaac accaagaaat atcaagcaaa atcgtaattc cacttgatcc
     2881 ttttaaaaca catcgagagt atttgcagag agctaactaa acaagcctat ccagtttata
     2941 taaacaaaaa actccaatta caatcaagaa ttagagttga cttacaagat tagaccgttc
     3001 atttcaccat acgaaaaaac tgttcacatt tcccttcgcc agtcttaact gtatcaggtt
     3061 caatgggtat tatctcagcc taaagcaccc caaatgtctc tattattt
//
LOCUS       DAVKGC010000144         2992 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKGC010000144 DAVKGC010000000
VERSION     DAVKGC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.144"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            14..556
                     /locus_tag="VW049_001928"
     CDS             14..556
                     /locus_tag="VW049_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9432984.1"
                     /translation="MQESNKRLKTKQTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(597..670)
                     /locus_tag="VW049_001929"
     tRNA            complement(597..670)
                     /locus_tag="VW049_001929"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(634..636),aa:Asn,seq:gtt)
     gene            complement(676..747)
                     /locus_tag="VW049_001930"
     tRNA            complement(676..747)
                     /locus_tag="VW049_001930"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:Glu,seq:ttc)
     gene            complement(799..1551)
                     /locus_tag="VW049_001931"
     CDS             complement(799..1551)
                     /locus_tag="VW049_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9432985.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1548..2873)
                     /locus_tag="VW049_001932"
     CDS             complement(1548..2873)
                     /locus_tag="VW049_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9432986.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2894..>2992)
                     /gene="comC"
                     /locus_tag="VW049_001933"
     CDS             complement(2894..>2992)
                     /gene="comC"
                     /locus_tag="VW049_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9432987.1"
                     /translation="FVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
BASE COUNT         1031 a          564 c          420 g          977 t
ORIGIN      
        1 acaaggagga aatatgcaag aaagtaacaa acgcttaaaa acaaagcaaa ctattgaaaa
       61 tgctatggta caattactga tggaacagcc atttgataaa atttctactg tcaaattagt
      121 agaaaaagcc ggaattagtc gttccagctt ctatactcac tataaggata agtatgatat
      181 gattgagcac tatcaaagca agctattcca tacatttgaa tatatttttc aaaaacatgc
      241 tcatcacaaa agagacgcta ttttagaagt ttttgaatat ctagagtcag aaccacttct
      301 ggctgccctt ctttctgaaa atgggactaa agaaatccaa aatttcttac gaaataaact
      361 tcatatcatg cttagtacag atttacaaaa gcgatttatg caactgaatc taaataccac
      421 cgaattagaa tatagtagca tctatctaac tcatgcactt tttggtgtct gccaaacttg
      481 gattgcacat ggaaaaaaag aaagttctca agaaataaca gacttcctta tgaaaatgct
      541 tggtgataca aattgataca aaaagaggaa cacagctgtg ttccctttta tctatactcc
      601 gccagtagga ctcgaaccta cgacatcatg attaacagtc atgcgctact accaactgag
      661 ctatggcgga taaaatagtc cgtacgggat tcgaacccgt gttaccgccg tgaaaaggcg
      721 gtgtcttaac cccttgacca acggaccttc tatctgtagc agatataacc attatatcaa
      781 tttcttgcta attgtcaatc acttttgaga ttttttctct aaaatatctt ttaattttct
      841 aatttttaat cttgaaatag gacaacgatg gtcttcatag aaaacaattt ctaagttttt
      901 tcgatcaatt tctctgatat tgcctatatt taccaaaaat gacttgtgag gagaataaaa
      961 tcgctgagta tgtttgtcct tttcctgaat atctgtcatg gtaccataaa actcttttgc
     1021 aaaattctta ccaataatgc gcaatttatg agagacccct gttgtttcaa tatacaaaat
     1081 atcatggtaa ggaattttta aatcatttcc cttgtaattg tagtcgaaat aatctacaac
     1141 atcttcattt tcaagtaaca tactcttcgt gtagaagata ttttgctcaa ttctcttctt
     1201 aaacatctca tcattgatat ccttatcaac aaaatctagg gctgatacct ggtatttata
     1261 ggttagagtc gcaaactctg atcgactagt gataaagacg ataatagcgt aaggattgta
     1321 atgacgaatg agctgagcca cttcaaatcc ctttttctca attccatgaa tatcgatatc
     1381 taggaaataa agctgattta cttcatcatt ttcaatgtat tcttcaaatt cacggacttt
     1441 tcccgttgtc ttgtatgata ttggaatatt cgattctttc gaaatttcat ccaatattct
     1501 ctctagtctc acttgatgtt caataacatc ttctaaaatt aaaactttca ttcaaattcc
     1561 ctcttaaatc taatgatttg tctaaatgta ctgccttcca tctctgtttc taaaataata
     1621 ttgttgtact tatctagtag ttctttcaca ttatttaatc cgactccgcg atttcttccc
     1681 ttagtggaga atcctaaggc aaatagatct cctgaaggag tcatcgtcat tttacatgaa
     1741 ttctgaatca caataactgt ttcagtttcc atcttaataa ctgctacttc catctgcttt
     1801 ttatagctat cagccgatcc ttcgacagca ttattcaata aaacgctcat gatacgaacc
     1861 aaatccaata gttcaattgg aagcttggta atcgtatctt ttacttccag tgtaaactct
     1921 acaccattat ttcgagcata gacaattgac tgagcaacca aacttcgtaa agctgagtct
     1981 tctatgttgt tcaaatcaaa gtaagtgtac ttatctgaac gcaatttatg atttgctttg
     2041 actaaaactt cattgtaaat tctgtcaatt tcctgtaaat taccactgtc aattgccatc
     2101 tgcatgctga caagcattcc agcataatca tgtcgaaaac cacggatttc attatacaga
     2161 ccaacaattt catctgtgta attctgtaaa tgtttctgtt caaatttctt ctgcttcaaa
     2221 gcaatctctt tctccatttg aactttatga gaattcattg caaagaaggt caaaaggaga
     2281 gagataaaga caatagatga caaaatactt ccaaaactat tcaaatgttt aatcgtactt
     2341 accatatctg aaacgaaaga tacaatatgt agcaatagta aagcaaaaaa tacttttttc
     2401 aagaaaggat aaaggtagtc ctcgtcaaaa taggctagtt ccaaatggaa atagtaaatg
     2461 atttttaatg taacaaaata ggttaacacc gtcacaacga aaaagaatgg gaaatgatat
     2521 tgtaaaacaa aattatctcc tgttatagag gagaaagtta cggacagaaa gttatgagtg
     2581 ctctcatata aaagagatag tagtaaactt aggaatagtc ctctatccct ctcatactgt
     2641 ttcatccatc gaaaatagga atataatccc aaaggaaata aaaatctttc aatccctaac
     2701 ccatctaaat atagaatata taagggaaaa tcaaatacta tttctgttag taatgtataa
     2761 gcaccaaaaa cgaataattc ttttctattt atttgacctt tacaaataaa atggtaactg
     2821 tgactaataa ttaaaaaatg aacaataacc gtcccaaatc caaataaatc cattactctt
     2881 tccccttatt tcattacttt tttctttgta aaataaaatc acggaagaat tttgacaacc
     2941 tcatctcccc acctttaatc ttttgtaagt ctttttcctt caaagctaca aa
//
LOCUS       DAVKGC010000145         2931 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVKGC010000145 DAVKGC010000000
VERSION     DAVKGC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.145"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(59..373)
                     /locus_tag="VW049_001934"
     CDS             complement(59..373)
                     /locus_tag="VW049_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEU9432988.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            complement(503..742)
                     /locus_tag="VW049_001935"
     CDS             complement(503..742)
                     /locus_tag="VW049_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEU9432989.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            complement(794..2230)
                     /locus_tag="VW049_001936"
     CDS             complement(794..2230)
                     /locus_tag="VW049_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9432990.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSLAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWKWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMIHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            complement(2420..2758)
                     /locus_tag="VW049_001937"
     CDS             complement(2420..2758)
                     /locus_tag="VW049_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU9432991.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQEALDEHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
BASE COUNT          977 a          609 c          358 g          987 t
ORIGIN      
        1 tatcaaactc aaaacaattt atcttaactt aaatatttat tgacatttca tgtgttcatc
       61 aaatattctc aagaatcaaa ttagccattt tttcaattcc cattggaata ggaatatagg
      121 cttgaggagg tatttgtaca actggttttc ctgctttaga accagcctct tcaaattgct
      181 taaagtacat ttttgtttga ggactgacaa gatacaaatc aaaagctgct gctgcgatag
      241 ctttccctcc ttcagtagca ctaatagcat caactacaat atctttccct tttcctttta
      301 gaaactctgt tgttttctgt gccataagtg atgaagacat tcctgctgca caaataatta
      361 aagcttttgc cataatattt tctccttttc ttaaatccaa tcaaagctgt gctaagttgg
      421 cttatttgtt atctattttt attataaaat aaagcgtttc caatgacaat tccctcattt
      481 tcctaaatga tatggaaaaa aattatttat acttcaattt ataaaataaa attattcctg
      541 agagtagaaa tgaaacacta tttgctaaaa tcaaaggcaa gtctcctata cgaataccat
      601 gagcaagcca caatgcaata ccaataactt gcataacata catacctaga gcaatagatc
      661 ctgtgtcctt tgtcttaact acacgaaaaa cttgtggtaa aaatgcaaat gttgttaaaa
      721 ttgctgcaat acttccaatc atatgtcacc ccaatatgct aaacaaactg agaataatct
      781 cagtttgttt atactattct actgattcac cgttagatga aataacttcc ttataccagc
      841 caaaagattt tttcggggaa cgattataac ttcccttccc attatcatct ttatctacat
      901 aaataaagcc ataacgtttc cgcatttcac cggtaccagc tgaaaccaaa tcaatacatc
      961 cccatggagt ataacccatt aaatcaacac catcttcaac tacagccttt ttcatttcac
     1021 gaatatgggc acctagatat tcaattctat aatcatcatg tatcatacca tctgctgcaa
     1081 cttgatctat agctccaaaa ccattttcaa caataaagag tggtaagtga tagtggtctg
     1141 taaaccaatt taacgcataa cgcaaacctt ctggatcaat ttgccacttc cattcagaag
     1201 ccttaacata attatttttc actaaatctt ctgtttcaag ataatcaaaa taaggattat
     1261 tttcacgatg agagtctata gcaaaggaca tatagtaact gaaaccaatg taatctacag
     1321 tcccaccaag taaatcttct ttatcctggt cagtaaaatc aactgaaata ccttttcgtt
     1381 cccaatactt gaaaatatgc tcaggatatt tacctaaaac atgcacatca gcaaaataat
     1441 aacgcttctg catagctttc attgccatta agatatcctt aggattgcaa gtaactggat
     1501 aaattggaca catcgcaatc atacaaccta tttgaaaatc tggattaatc tcatgaccaa
     1561 tttttacagc tcgtgcagaa gcaactaatt cgtaatgtgc tgcttgatac ataattgcct
     1621 ctctattatc accttcctca tatacaatac ctgagttagt aaatggtgca aaatcttcct
     1681 gataattcgc ttgattattg atttcattga aagtcatcca atatttaacc ttatctttgt
     1741 aacgtttaaa tacgacttct gcaaaacgag caaagaaatc aatcaatttc ctatttttcc
     1801 aaccaccata ttcggtcact aagtgataag gcatttcaaa atgagataga gtgatgacag
     1861 gttcaatacc attctttaag cattcatcaa aaagattatc ataaaactgt aatccttctt
     1921 cattcggctc taactcatca ccttttggaa agatacgtgt ccatgcaaga gaggtacgga
     1981 agcacttgaa tcccatttca gcaaaaagtg ctatgtcttc tttataacga tgataaaaat
     2041 ctatcgcctc atgatttgga taatatttac cctctaaaac tcccaaagta atttcacgag
     2101 ctactccatg acgaccagca gtcataacat cagcaacact aattcccttg ccaccttctt
     2161 gccatccacc ttcaagttga tgagcagcaa cagcaccacc ccataaaaat ccatctttaa
     2221 aagtagtcat cttttttcct cctgactttg atactcttat tataaacctc aaatcaaaag
     2281 atgaaaacgc attctttttc cttattgtta aggaaagaag taatttttaa tggaaataga
     2341 acaatatctt cttgtattct cgtaatgata tctttacgat tttcaatact ttcaaactac
     2401 aaaaactctc acaataattc taattccctg tgtctataaa cgacttatcg ctttctggca
     2461 tcccagaatc atcttctata taacgttcaa cttgcatctg caagtgatat ttttttctta
     2521 aatctaagat tttctgcatt gtctttgatt gatgatgttc atctaaagct tcttgattta
     2581 tccactgatc aataaggaga atagttccct ctttttcaat tggtaaaaaa tattcgtatt
     2641 tcaagttacc tttttgattt ctaatttctt caacaaggcc actatcaagc atttctcttg
     2701 caaactttat tgcactatct ccatcacctt tataatatac atgaatagtc aatgtcatct
     2761 tatatcctcc aaaatcatcc ttcaatttta aaaaaacaag tttagatgag gatctaaact
     2821 tgttttttat gaactaatta tctaacgttt cgccattact ttcaatcact tctttatacc
     2881 aataaaatga ttttttctta tagcgattta tagtcaattg aaacaagagc a
//
LOCUS       DAVKGC010000146         2886 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVKGC010000146 DAVKGC010000000
VERSION     DAVKGC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.146"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..1343)
                     /gene="rlmD"
                     /locus_tag="VW049_001938"
     CDS             complement(<1..1343)
                     /gene="rlmD"
                     /locus_tag="VW049_001938"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9432992.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLDYEFSL
                     SPRAFYQLNPEQTEILYGEAVKALDVSKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDIPYIQSVDMFPHTARTEAVVKL"
     gene            1381..2157
                     /gene="recX"
                     /locus_tag="VW049_001939"
     CDS             1381..2157
                     /gene="recX"
                     /locus_tag="VW049_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9432993.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2246..2779
                     /locus_tag="VW049_001940"
     CDS             2246..2779
                     /locus_tag="VW049_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9432994.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          920 a          534 c          531 g          901 t
ORIGIN      
        1 attaatttta caacagcttc agttcgagca gtgtgtggaa acatatcgac cgactgaata
       61 taagggatat catagacttc tactaacctt accaaatccc ttgccaaagt tgaaacattg
      121 caagaaatat agaccatttt ttctggtaca taggtaagaa ttgtatctaa taacttgtca
      181 tccagacctg tacgtggtgg gtcaacaatc aaagcatctg ctcggtagcc ttccttgtac
      241 caacgaggaa taatctcttc tgccgttcca gcttcataat gagtattgtc aaatcccatt
      301 cttttagcat ttcgcttggc atcttcaata gcttctggaa taatatccat acctctgagt
      361 gtttttactt tctttgcaaa ggcaaatcca atcgtaccaa caccacaata cgcatcaatc
      421 agatggtctt ctttgctaac atccagagcc ttgactgctt ctccatagag aatttctgtc
      481 tgctcaggat taagctgata gaaagctcga ggggagaggg aaaattcata gtcaagtact
      541 ccttcttgaa tactctcttg cccccagata atctctgtct tttcaccata tatctcactg
      601 gttttagctg tatttgtatt aacagctact gtcacaactt ctggaaaatc tttaaccaac
      661 tcttttacca gttgggttaa attaagctgg cggtttgtaa caataataat ctgaacctgt
      721 ccggtctttc tcgcgcgtcg gaccataata gtacggacac ctagaacttt tctctcatcc
      781 gtgattggaa tctggtgata agtaagtaat tctgctaagc gattagcaat cacttgggtt
      841 tccttatctt gtaccaggca gtctttcaac tctactaaat agtgagagtt ttgtgcatat
      901 aagcccgcct tgacctgatt tttaaatttt cgagtctgaa attgtaactt agctctgtaa
      961 tattttggtt cctgcattcc aatagttgga cgaatttcat aattttcata tcctgcagga
     1021 gcaaattttt tcagcgcttg atgaagtaag tccgtcttga actccagctg cttatcataa
     1081 tgcaggtgca tgatttggca gcctccgcat tcattataaa tagtacaaga tggcacaatt
     1141 cgaaatttag acttcttgtt gaccttcagt aattttgctt caacaaagtt gcgtctaata
     1201 gaagtaatct gacaatagat atcttcgcct ttgagagctc ctggtacaaa gactaatgtt
     1261 ttttggtaaa agccgattcc ctcaccgtta attcccatgc gcttgatttt taatggtatt
     1321 ttttgtttca ctttcagatt cataccccta tcttatcaca ttttgagtta taatagagct
     1381 atgaaaatca caaaacttga aaagaaaaaa agactctatc tgatggagct tgataatggc
     1441 gacaaatgct atatcaccga agatacaatt gttcgtttta tgttatcgag agataaggtg
     1501 ataagcgaag aggaattgaa agagattcaa gactttgctc aattttctta tggtaagaat
     1561 ctggccctct accacttatc ctttaaagca cgcactgaaa aagaagtcag agaatatctg
     1621 aaaaaatacg atattgataa aaacatcgtt tctcaagtta ttgctaatct taaagaagat
     1681 aagtggatta atgatggtca gtacgcttat gctatcatca ataccaatca actttcagga
     1741 gacaagggtc cttatgtgct gactcagaaa ctagctcaaa aagggatttc aaaatctact
     1801 atagaagaga acttgaaaga atttgatttt tctgaagttg ctcaacgtgt agctaataaa
     1861 ctattgaaaa aatatgaggg aaaacttcca gctcgtgcct tgcaagataa gattatccag
     1921 aacttgacta acaagggctt ctcttattct gatgctaaaa ttgcctttga cgacttagat
     1981 agccaagttg accaagaaac gactcaggaa ctcatcttca aggaacttga taagcaatat
     2041 actaagtatg ctcgaaagta tgaaggatac gaactgaagc agcgtttaac tcaagtttta
     2101 gcacgaaagg gctacgattt ttcggatata gcaagcgctc ttagagaata tctttaatat
     2161 ttccaagtga aattcacaga ttttaggtaa ttttatggta caatagtaaa tgataaactt
     2221 ataaattgta gaaagttggt tagttatgaa gcttccaaaa gaaggcgact ttattacaat
     2281 tcaaagttat aagcatgatg ggagtctcca ccgaacttgg cgggacacca tggtactaaa
     2341 aacaacagaa aacgccatta ttggtgtcaa cgatcataca cttgttaccg aaagtgatgg
     2401 tcgtcgttgg gtcactcgag aaccggctat tgtttacttt cacaagaaat attggtttaa
     2461 tatcattgcc atgattcgtg ataatggaac ttcttactat tgcaatatgg ctagccccta
     2521 ctatctggat gaagaagcac tgaagtatat tgattacgat ttggatgtta aaatttttac
     2581 agatggggaa aaacgtctct tggacgttga agagtacgag cgtcacaaac gcaaaatgaa
     2641 ttattctgat gacttggact atattttaaa agaacatgtc aaaattcttg ttgattggat
     2701 taacaatgga cgtggtcctt tctcagaagc ctatgtaaac atttggtaca agcgctatgt
     2761 agaactaaag aatcggtaaa gttgtcaaag gtcaggaaca aaatcctgac cttttttggt
     2821 agtataaaat aaattgtgta aacacaaaaa ggaataaatc tattatagca gagttacaac
     2881 acatta
//
LOCUS       DAVKGC010000147         2816 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVKGC010000147 DAVKGC010000000
VERSION     DAVKGC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.147"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            6..77
                     /locus_tag="VW049_001941"
     tRNA            6..77
                     /locus_tag="VW049_001941"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Gln,seq:ttg)
     gene            239..1441
                     /gene="rpsA"
                     /locus_tag="VW049_001942"
     CDS             239..1441
                     /gene="rpsA"
                     /locus_tag="VW049_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9432995.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1527..2816)
                     /locus_tag="VW049_001943"
                     /pseudo
     CDS             complement(1527..2816)
                     /locus_tag="VW049_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          854 a          464 c          636 g          862 t
ORIGIN      
        1 attaatgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
       61 gaatccagct accccagttc ttaggtaata atcaagataa aaagcaaaat atcttagggt
      121 attttatttt tataattgaa agacgtgaat gatatgaaca tgtccttgcg ggtgcttagg
      181 aaaaaaatta taagtatgtc aagtttaaga aaaacttgat tgttggagga ttttttagat
      241 gaacgaattt gaagatttgc taaatagcgt tagtcaagtt gagactggtg atgttgttag
      301 tgctgaagta ttgacagttg atgcgactca agctaacgtt gcaatctctg gaactggtgt
      361 tgaaggtgtc ttgactcttc gcgaattgac aaacgatcgt gatgcagata tcaatgactt
      421 tgttaaagta ggagaagtat tggatgttct tgtacttcgt caagtagttg gtaaagatac
      481 tgatacagtt acataccttg tatctaaaaa acgccttgaa gctcgcaaag catgggacaa
      541 acttgttggt cgcgaagaag aagttgttac tgttaaagga acgcgtgccg ttaaaggtgg
      601 actttcagta gaatttgaag gtgttcgtgg atttatccca gcttcaatgt tggatactcg
      661 tttcgtacgt aatgctgagc gttttgtagg tcaagaattt gatactaaaa tcaaagaagt
      721 taacgctaaa gaaaaccgct tcatcctttc acgtcgtgaa gttgttgaag cagctactgc
      781 agcagctcgc gctgaagtat tcggtaaatt ggctgttggt gatgttgtaa ctggtaaagt
      841 tgctcgtatc acaagcttcg gcgctttcgt cgaccttggt ggtgttgacg gattggttca
      901 cttgactgaa ttgtcacatg aacgtaacgt atcaccaaaa tcagttgtaa ctgttggtga
      961 agaaattgaa gtgaaaatcc ttgatcttaa cgaagaagaa ggacgtgtat cactttcact
     1021 taaagcaaca gtaccaggac catgggatgg cgttgagcaa aaattggcta aaggtgatgt
     1081 agtagaagga acagttaaac gtttgactga cttcggtgca tttgttgaag tattgccagg
     1141 tatcgatgga cttgttcacg tatcacaaat ttcacacaaa cggattgaaa atccaaaaga
     1201 agctcttaaa gttggtcaag aagttcaagt taaagttctt gaagttaacg cagatgcaga
     1261 acgcgtgtca ctttctatta aagctcttga agaacgtcca gcccaagaag aaggacaaaa
     1321 agaagaaaaa cgtgctgctc gtccacgtcg tccaagacgt caagaaaagc gtgatttcga
     1381 acttccagaa acacaaacag gattttcaat ggctgatttg tttggtgata tcgaacttta
     1441 atcaaattga aaattcacaa aatcctttgt ttactaaaca agggattttt ctggctcttt
     1501 gtcaactgta gtgggttgaa aaaaagctaa gctcgagaaa ggacaaattt cgtcctttct
     1561 tttttgatat tcagggcgat aaaaatccgt tttttgaagt tttcaaagtt tcgaaaacca
     1621 aaggcattgc gcttgataag tttgatgaga ttattggtcg cttccagttt ggcattagaa
     1681 tagtgtagtt gaagggcgtt gataatcttt tctttatctt tgaggaaggt tttaaagaca
     1741 gtctgaaaaa taggatgaac ctgcttaaga ttgtcctcaa taagtccgaa aaatttctct
     1801 ggttcctttt tctggaagtg aaaaagcaag agctgatagt ggtgtttcaa gtcttgtgaa
     1861 tagctcaaaa gcttgtctaa aatctcttta ttggttaagt gcatacgaaa agtaggacga
     1921 taaaaacgtt tatcactcag tttacggcta tcctgttgaa tgagtttcca gtagcgcttg
     1981 atagccttgt attcatggga ttttcgatcc aattgattca tgatttggac acgcacacga
     2041 ctcatagcac ggctaagatg ttgtacaatg tgaaagcgat caagaacgat tttagcattc
     2101 gggagtgaaa cagtctggga gactgtttca gcctgagcct agaaatttga aagcgaagct
     2161 gtttagccaa gtcatagtaa gggctaaaca tatccatagt aatgattttg acgcgacatc
     2221 ggacggctct atcatactta agaaaatgat ctcggatgac agcttgtatt ctaccctcaa
     2281 gaacagtgat gatattgaga ttgttaaaat cttgcgcaat aaagctcatc tccatctccc
     2341 gattgaaaca gtcactcccc ggactgtttc aacctcccaa gacataatct caggcagatt
     2401 tctaaaatta cactcaaagt ggaagtcatt gagcttgcga atgacagttg aagttgaaat
     2461 ggccagctga tgggcaatat cagtcataga aatcttttca attaactttt gagcaatctt
     2521 ttggttgata atacgaggga tttggtgatt cttcttgacg atagaagttt cagcgaccat
     2581 catttttgaa cagtgatagc acttgaatca acgctttcta aggagaattc tagtaggcat
     2641 accagttgtt tcaagataag gaattttaga aggtttttga aagtcatatt tcttcatttg
     2701 acttccgcac tcagggcaag atggggcgtc gtagtccagt ttggcgatga tttccttgtg
     2761 tgtatcttta ttgatgatgt ctaaaatctg gatattaggg tctttaatgt ctagta
//
LOCUS       DAVKGC010000148         2814 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVKGC010000148 DAVKGC010000000
VERSION     DAVKGC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.148"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            6..1979
                     /locus_tag="VW049_001944"
     CDS             6..1979
                     /locus_tag="VW049_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9432996.1"
                     /translation="MLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLKLI
                     EKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVFIL
                     NKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSGEE
                     RDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDFVL
                     QNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQEID
                     YITLHLLAKSQQCQKNQKNISEEVLKKSLLKTFQNLGLDDMYNFSSDFQLIEGLITHL
                     MTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVSLH
                     FLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQEKL
                     KGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINKFE
                     NNGFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSSVV
                     FSEKIAVPHPIQSFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLATVT
                     KKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            1989..2303
                     /locus_tag="VW049_001945"
     CDS             1989..2303
                     /locus_tag="VW049_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEU9432997.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            2334..>2814
                     /locus_tag="VW049_001946"
     CDS             2334..>2814
                     /locus_tag="VW049_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9432998.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LSQFISPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     "
BASE COUNT         1004 a          363 c          521 g          926 t
ORIGIN      
        1 tgaatgtgtt aaacattaaa gagaaagata tccttcaatt tttaataaaa aataaggaac
       61 gttttgttac aagcaaagaa ctggcagaat atttatcctg ttcagataga actgtccgta
      121 atgtactgaa attaattgag aagacaatga ttattcaagg agtccggttg atttcaaaac
      181 aaggtcaagg gtatcaaata ttttttgaga atcaaggcgc ttaccaggag tttagacaaa
      241 cttatgaact ggaagaagac tatacgaaaa ctgcggtttc aaaaggtgat gatcgtttag
      301 tatttatctt aaataaatta ttatttgagc aagttccagt tctttttgat gatttagctg
      361 atgaacttta cgttagccgt tcaacattat ctcatgattt cagaaagata cgagtgatgt
      421 tatcagagta taatttgtcc attgagagta gggcgaataa aggagtttat gtctcaggcg
      481 aggaacgtga taaacgacgt tttatcataa attatttcct agagaatcaa ttttttaaaa
      541 caatacattg ctatgtaaaa tttaactttt ttgatcaaac tgttccatta gaagaatttg
      601 ctagaattgt tttggatgaa tgccaagagg ccaacctaaa actatctgat tttgtactac
      661 agaatttagt agttcacata gcattgtctg tgattcgttt aaaatccgga tttgaaataa
      721 aaaatataga ctgtcagatg actgatgatg caatagagag aaaggttgcc caaagaattc
      781 tttctaaagt aagagaagta acaaatcagg aatttccagt tcaggaaata gactacatta
      841 ctttgcattt gttagctaag agtcaacaat gtcaaaaaaa tcagaagaat atttctgaag
      901 aagttttaaa aaaatcttta cttaaaacat ttcagaattt aggattagat gatatgtata
      961 acttttcttc agatttccaa ctaattgaag gattgataac tcatctaatg acactacaag
     1021 tacgtttaga aagccgaatt accttaaata accctttggt agatgaaatt aaacagaatt
     1081 atagtgatat attctttatg actagagaaa ttcttgcaaa tatggacaag ttcttggagt
     1141 ggacaatatc agatgatgaa gtagcatatg taagtcttca ctttttagct gctatggaac
     1201 gtagcaaaga gagtactaag tttaatattc ttgctatttg tgcaacagga tttggtgcag
     1261 cccaaatgtt aagaaatcgt ttggaaacgg agtttgggaa gcgagtagaa gttgttgatg
     1321 taataggcta ttatgaattg aatcaagaaa aacttaaagg aatagatttt atagtgtcag
     1381 ctgttgattt atcaaatcta tattttcaaa tcccagtttt taaagttagt gtgtttctta
     1441 agagtgatga gatggaaatg attagaaaag ctatggatca gatgcaagtt tctagttatg
     1501 tacaatcatc aaagattaac aaatttgaaa acaacggctt tagacagtat tttagtaaag
     1561 aaaacttttt aatatgtact gaatcagaca aggtaaattt acttgaaaag atggttgaga
     1621 gtttatctgt tggtgaatca aatgagtttg aacaatcttt actttatgga attaaacagc
     1681 gcgaagaatt aagttcagtt gttttttctg aaaaaattgc agttccgcat cctattcagt
     1741 cctttggtac agagggaaag gtttcggttg caatttgtaa agattcgtta ctctgggata
     1801 atcaaagttc acatgtccag ctagtatttc ttctatctcc atcaatatat gggaacgaag
     1861 gtttggcaac tgtgactaaa aagatagtct ctctgacaga gaatgatgaa ttgcaaaatc
     1921 agctaatatc ttgcaataac tttgaagatt ttattaacat atttgagaag ataaaatagg
     1981 aggttcatat gaatcaagag gaactgcagg ttgcagcatt tgaaattatc ctgcattcgg
     2041 gaaatgcaag gtcagaaata catgaagcat ttgcaaaaat gagagaaggt agttttgatg
     2101 atgcagaaag taaattaaat caatcaaatg agataattct tgaagcgcat catgcacaaa
     2161 ccaaactctt gcaagaatac gcaagtggag tggaaattaa aattgaaatt atcatggtac
     2221 atgctcaaga tcatttaatg accactatga cattattaga agtagcaaag gaaatgttag
     2281 ctctttataa gaaagttaac taaatatgtg ttgttatata aggaggaaat gatatggata
     2341 aaaaagaacg acaaaagatt gaacaacaac gtcgagagat ggctctaact aatacttttt
     2401 tcaatcgata tcttcttcta cgttattcaa tagcactctt cttttttggg aatatttatt
     2461 ggcttttgag tcagtttatt agtccctcgc ctattattat tttccccatt atgctcatag
     2521 tattttctat tttagcaact gttgaacaat ttaaactcta tggaaatagg aaagaaaagt
     2581 tgggaataac actaatgttt gttagaatac aaatgcttat ttcaatagga ttacttgttt
     2641 taacttggac aagttggttt aagaatcttt ttccgatttt tgaaaataac caagtagcac
     2701 gtttatttgt ttttgtagtt cttctattag gtttagttct gagtttgtta gacattagaa
     2761 gaattaaaaa aatttataaa cgaacagata aggcttatca acaatttgtc caat
//
LOCUS       DAVKGC010000149         2732 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVKGC010000149 DAVKGC010000000
VERSION     DAVKGC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.149"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            46..573
                     /locus_tag="VW049_001947"
     CDS             46..573
                     /locus_tag="VW049_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9432999.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYLLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKQGKENGMV
                     ALLRMDINLKTASCL"
     gene            564..1724
                     /locus_tag="VW049_001948"
     CDS             564..1724
                     /locus_tag="VW049_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9433000.1"
                     /translation="MFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQN
                     GKNPLFDAGLIRKILKNPVYNGKIVFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEA
                     IVSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCRICGVGMFGNKCIKKKK
                     DGTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQE
                     KINMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLY
                     RMYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISA
                     LISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            1992..2558
                     /locus_tag="VW049_001949"
     CDS             1992..2558
                     /locus_tag="VW049_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433001.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT         1021 a          337 c          606 g          768 t
ORIGIN      
        1 aaaaaattat atatgatata gaaaaattat attgaggtta atactatgtc aaaagaaaaa
       61 ataaaagtat acctctatac acgagtatct acgtcaatac agatagaggg gtatttgtta
      121 gaggcacaaa aatcacgaat gaaagctttt gctatttaca atgattatga aattgttgga
      181 gagtatgaag atgccgggaa gtctggcaag tctattgaag gtagaataca gttcaatcga
      241 atgatggaag atataaaatc tggaaaagat ggagtatctt ttgttcttgt gtttaagtta
      301 tcaagatttg caagaaatgc cgctgatgtt ctttctacac ttcagataat gcaggattat
      361 ggagttaatc ttatctgcgt tgaagatggc attgattcat ctaaagatgc tgggaaacta
      421 atgatttctg ttttatcagc tgtggctgaa atagaaagag aaaacatccg tattcaaaca
      481 atggaaggtc gcattcaaaa gcaagggaag gaaaatggaa tggtggcttt gctccgtatg
      541 gatataaact tgaagacggc aagctgttta taaatgagga agaggcagtt gcaataagaa
      601 cgattttcga ccagtatgta aatactacga taggagccaa tgggatatct aaatacttag
      661 agaatcatgg aattagaaaa atcccaagac agaatggtaa gaatcctttg tttgatgcag
      721 gtcttataag aaagatatta aagaatcctg tatataatgg gaaaatagta tttggaagaa
      781 gaactttaga aaaagttcat ggtacaagaa atgaatataa gcaggttgaa caagatgaat
      841 atctaatatc tgaagggata catgaagcta tagtttccga tgaagtttgg caagctgctc
      901 aggttaagct aaaatctcaa gcaaagaaat atgagcatgt gaataaagga aaagatacac
      961 gcacacactt gctttctgga attgtaaaat gccggatatg tggagtggga atgtttggga
     1021 acaagtgtat caagaaaaag aaagatggca caaagtataa agatttttat tactatggtt
     1081 gtaaacatag gcagatgata agaggtcata agtgtacatt cagtaagcaa attagagaag
     1141 aattgttaga tgatgctgtt gcagaggtga ttgtcaagat agtaagtaat ccgaaatttg
     1201 cttctatgat gcaagaaaaa atcaacatga aggtggatac ctctgaaata gaaaaagaaa
     1261 tagataatta ccaaaaggaa ttgagaaaga gtcattctac aaagtttaag ctaattgagg
     1321 aaatagataa tttagatgtt gaagataagc actataagag aaggaaacag gatttagatg
     1381 atagactata tcgtatgtat gacaaaatag atgaattaga atcatcacta attgatgcga
     1441 aagcaaagaa acagactatt gaagctgaaa agcttacagg agataacata tataaggttc
     1501 tgatctattt tgataagctc tataaagtca tgaatgatgt agagcgtagg cagttaattt
     1561 cagctttgat ttctgaaatt caagtttatg aagaaaaaca atctaacgga caatggctaa
     1621 aatccattac ttttaagcta ccaattatag aagaaaatct caacataggt ttggacaatg
     1681 atgaacaagt tgaatgtgta agtttattag agaaacgtag ttaatcctca aaaggttccc
     1741 cgaacctttt gagtcccact gacgcatcac gctgaggcgg tatcactgct tgtacgagtt
     1801 gaagcatcag cgaagtaaaa gtagatgttc agccaatgca acgaggtgag accgaaggtc
     1861 gataaggtgt gttctgggta taggacttgt aaagtaagta ggaaatatga tgagatgata
     1921 cagagagcct gaattggtca ggtgacgtgc ttatttacat tagcctgata atgaaaatgg
     1981 agagattatc gatggttata ttggaattgt accaaaatga ttattctaaa gatttagtag
     2041 catttgattc catagaagat gggaaagcct ttgtagctca aatcccggga tacactctgg
     2101 aaacagaaga tggttttgaa gtggagtatt ttaatcctaa aaacataccg gattatatgg
     2161 aaattatttt taacggaaat attgttcctt tatctaaatt tatgttcgat cctggggaaa
     2221 atgtaaacat tatttggaaa gagatttcaa atttatccct caaaaatgat agagtgatcg
     2281 aaggatattc aaaaattgat gcatatgtcg ttaacaacca tgaagttaaa gcctatgtcg
     2341 aaacaagaga aacgaactat cgtaaagcaa aagacttctt agaaagccgc ggatatgaaa
     2401 tcgacagaag ctttttcgga agtgaagacg gtgaagcgat tctttacaga aaaaaaggga
     2461 tcgaagtttg gcatttcttg tgtcacttag acccaatgtt tgtagagatt gaagatgtag
     2521 aaggatatgt taaagaagaa gttggagaga ttcaatagtc aaccaaagtt ccttgtgagt
     2581 atataccttt gctagtaaaa gagtcaaaac gttataacga aggggggggg aagaaaatcc
     2641 ttgagtgtaa gtctttttct tgtttagagg gaaagtatta gagtagccgc tcataattta
     2701 tactagattt gataactgat ttgagacaga tt
//
LOCUS       DAVKGC010000150         2700 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVKGC010000150 DAVKGC010000000
VERSION     DAVKGC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.150"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(1..610)
                     /locus_tag="VW049_001950"
                     /pseudo
     CDS             complement(1..610)
                     /locus_tag="VW049_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(856..1167)
                     /locus_tag="VW049_001951"
     CDS             complement(856..1167)
                     /locus_tag="VW049_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433002.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1182..2468)
                     /locus_tag="VW049_001952"
     CDS             complement(1182..2468)
                     /locus_tag="VW049_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9433003.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          803 a          598 c          476 g          823 t
ORIGIN      
        1 ccattggatt tggagcgcgg tcgattttat tgcctttggc atcatgcaaa tcttcaatat
       61 aggtttcaaa atgacggaaa cctggaccat aaaactcaac ttggtcccct tcgttaatga
      121 catttcgttg acgaatagtt gctgtttgtg ccgcatcatc ataagaaacc acttcagcga
      181 caaacttgta ctcaggaatt ttacgacgag caccaaacaa ctgctcattt tcagatggtg
      241 taccatagta aaatcctgta gccagttcac gttgggcaac cttccacatc tcgtccacca
      301 agtcttgttt gatagcttca aacttttcag gactttcaag ataggcatcc acagccgcct
      361 tgtagcagtt ggttactgtt aatacatagt gaatagactc catacgtcct tcgattttta
      421 gactgtccac accattttca atcatatctg gaatgtggtc aatcatagac atgtcaacgg
      481 ctgacattga aaattcttct ggaatctcac cctgcaaact cttacgttct ttcccaaatg
      541 gcatatcgta aaggtcgtac ttccaacggc atgactgaga acatccacca cggttggcat
      601 cacgcatact ctagacggat tgttttgaca attctaatct tttagacttt gcataataac
      661 tgttaaacag ttgaaaattg acccagtttt aacaagtagt caaaatacca gataaaggaa
      721 aaaagtcttt aaaatcttaa aaacgcatag tatcaggtat tgaacaatct tgatattatg
      781 cgttttattg tgggaagatt tacttcattt tctcttggaa ttgagttttc tcctacttca
      841 aaactagatg atttgctatt taaaatagcg ttccttatga ttttggcaac ctggattaaa
      901 gcgagaaaaa caaaaggggc aacttaagct ttctttatat tgcttatatg ttagtagttt
      961 tagacaaaca ccacataaaa taggcttatc ctgtatcaaa gataagggat acggctcaaa
     1021 agggtgattt tctaatgaat catgacaccg ataacaagca taatactttt tacaatcata
     1081 gcattgtaaa gcaataatat ccagcttgct atggtaatga atgcatcttg tttgcttatc
     1141 cactaagaaa ccctgtgcct ctatcatttc cttttccttt cttaagctcg aactgtgacg
     1201 ctggttccat cttccttata aagattgatg agtccttctt ttaatgcacg aaccatgtct
     1261 cctgattgaa cgggttgagg caccttaata gtcaatagtt ccattggatt tggagcgcgg
     1321 tcgattttat tgcctttggc atcatgcaaa tcttcaatat aggtttcaaa atgacggaaa
     1381 cctggaccat aaaactcaac ttggtcccct tcgttaatga catttcgttg acgaattgtt
     1441 gctgtttgtg ccgcatcatc ataagaaacc acttcagcga caaacttgta ctcagggatt
     1501 ttacggcgag caccaaacaa ctgctcattt tcagatggta taccgtagta gaaacctgtt
     1561 gccaattcac gttgggcaac cttccacatc tcgtccacta agtcttgttt gatagcttca
     1621 aacttttcag aactttcaag ataggcatcc acagccgcct tgtagcagtt ggttactgtt
     1681 gatacgtagt gaatagactt catacgtcct tcgattttta gactgtccac accattttca
     1741 atcatatctg gaatgtggtc aatcatagac atgtcaacgg ctgacattga aaattcttct
     1801 ggaatctcac cctgcaaact cttacgttct ttcccaaatg gcatatcgta aaggtcgtat
     1861 ttccaacggc atgactgaga acatccacca cggttggcat cacgcatact catgtggttt
     1921 gaaagagtac aacgtccaga gtatgaaata cacatagctc catggacaaa ggcttcaatt
     1981 tcaacatctg tacgtttgcg gatttcagct aattcttcca ttgaaacctc acgcgctaaa
     2041 acgacacgag tcaagcctag ctctttccag aactcaaggg tttcatagtt agtggcactg
     2101 gcttgggtag aaaggtggat ttcaaggcct ggtgcttcag tcgctgcaat cataatcaag
     2161 gctgggtcag atacgataac tgctgcaatc ccgatatcac gcagtttacg gaaccactca
     2221 ccagcaccag cttcatttcc ttcgtgcata accatattag ccgctacata gaccttggca
     2281 ccatacttgg ccgcaaactg cacgccttct tccatctgtt cgaaagtaaa gtttcccgca
     2341 cggctacgaa gaccataggc ctgaccaccg ataaagacag catctgctcc atactgaaca
     2401 gctaccttta gcttctctaa agtccctgca ggtgataaaa cctcaggacg ttttaatgtt
     2461 tttgtcataa tttctcctat ttgcaatata aaagtttgac atttttcctt ctcattttat
     2521 agtaaataaa ggataaaatc aagcctagac ggattgtttt gacaattcta atcttttaga
     2581 ctttgcataa taactattaa acagtcgaaa attaccccag ttttaacaag tagtcaaaaa
     2641 gtctaaaaaa gtaaaaacca gtatcccaag atactgactc gtaaaaatat ataaattagt
//
LOCUS       DAVKGC010000151         2602 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVKGC010000151 DAVKGC010000000
VERSION     DAVKGC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.151"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..640)
                     /locus_tag="VW049_001953"
     CDS             complement(<1..640)
                     /locus_tag="VW049_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9433004.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLID"
     gene            complement(628..1185)
                     /locus_tag="VW049_001954"
     CDS             complement(628..1185)
                     /locus_tag="VW049_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9433005.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            complement(1086..1715)
                     /locus_tag="VW049_001955"
     CDS             complement(1086..1715)
                     /locus_tag="VW049_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9433006.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            complement(1726..>2602)
                     /locus_tag="VW049_001956"
     CDS             complement(1726..>2602)
                     /locus_tag="VW049_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9433007.1"
                     /translation="MKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLK
                     KFDIDLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDK
                     GQILIDGKDIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKL
                     ANCTDFIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVN
                     NEKKIQESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIY
                     KNLIEKTKMAEEFIY"
BASE COUNT          820 a          513 c          324 g          945 t
ORIGIN      
        1 tatctattaa aaatacacgg tcaactatgt ccatcaaata ataaattcta tgctctgcaa
       61 caattatgct tatgcctttc tcttttaata tctttagcat ctttgccaaa acacttatgc
      121 ttttaatatc taaattcgat gaagactcat ccataactat aatctttgta cctgctatat
      181 aagaagctgc aatgcaaaga atttgttttt caccgccgga tagattaaat atatttctgt
      241 tcaaaagatt ttttatcggg aatatctcaa gcatatcctt caaacgcctg tccatctctt
      301 ctcttgcaag accgatattt tccaaataaa ataataattc taatgtcgta ttgacattaa
      361 aaaaatatgt cttaggattt tgaaaaacag ttgaaacaag cattgagatt tgataaagtt
      421 ccaaattttt tatattctta ccatcaataa ttatttcgcc ctttgtcttt gcgttatcat
      481 accttacagc tagtccattg attgaattta ttaaagatga cttaccactt ccactttttc
      541 ctgtaaatag cacgcactct ccctcggcta tggatagtga tatatcatct aatatatgct
      601 cttcaccata atcaagactt aaatttttta tttcaaccat attaagcctc ccacaataag
      661 tccagcaacc gctaaaacat aaacaaaatc aattagctgt atctttacgc gaatgtatct
      721 ggtcttggca attggatttt ctattgcctt tgtttctgcc gcttttgcaa tgtcatctga
      781 tatattagat gatataatga gtagtggcac agaaacatat tcaagatatt tgactgggtt
      841 tttaaaattt atccctctta ctctcatagc cattttgatg tttttcttct cctccttaaa
      901 agatgggaag aatctaaaca taaccgcaat aggtatggat acattctttg aaatcttaag
      961 cttatccatc gatgaaatta ttgcgcctac atccgaggtc tttatcataa agcttgctgc
     1021 cataaatggt aaaataaaca ttctaataac aataggtata ctcaaaaaca tcttaactat
     1081 agggtttaat tcagatagca ccataaaatt aggcaaggag taaagtatcc cacacaatat
     1141 aatccatttg aaaagtgttt tttcaataga gccttgccta tatatgctcc aagaattcct
     1201 cctaagaaag cacctaaggc tactaaagcc atatgaggat aagttattaa cttttctaac
     1261 acatcaacat aatcttttcc catcgtcatc aaagaccact ccatatattt ttcttttgct
     1321 aaaagcattt gcattaaaga gctacatatc catgtgctga agattgcata agaaagcata
     1381 ttgtatttaa atgaattata attaccaatc tttctaatta attctgctat tatcattact
     1441 ataagtgata aaaccacaac tacgtaggta tgaccagctg caaacatcac aagtggtgat
     1501 atcataccaa atataaatag tgcccatggc ttttgaacct tagccataaa taacataaca
     1561 acagttcctg caactatggc taatactgtc gggtatatta agaacaatat tggaatcaag
     1621 cccatcatac caactccaaa catgaaggca aaataaatta cgccaaaaac accgatgctt
     1681 actaaatctt ttacttttaa ttttttatta tccattgtag tcctcctaat aaataaattc
     1741 ttctgccatt tttgtctttt ctattaaatt tttgtaaatt tttgattttt gtaaaagctc
     1801 ttcatgctta ccttcgcttt ctactcttcc gttttgaaga actactatct tgtctgcatt
     1861 ttctatggat ttcattctat gtgaaatgat tacaacagtt ttatctttaa ctaaattatt
     1921 taaagactct tgaatctttt tctcgttgtt aacatcaagg cttgctgtta tctcatctaa
     1981 gatcaatatc ggcgcatctt ttaagaaggc tctggctatt gataatcttt gtctttctcc
     2041 tcctgatagc tcagctccgt tctcaccaat aactgtatcg aaacctttat ccattttttc
     2101 tataaaatct gtgcaatttg caagttttgc tgctctttta acctcttcgt cacttgcatc
     2161 ttgctttccg attctaatat tttccataac gctttgatta aagagaacca catcttggaa
     2221 aactatagag accttatcaa aaagagattc tgttgatatt tcttttatat ctttgccatc
     2281 gattaagatt tgtcccttgt cataatcata aagtcttgat ataagtttca agatagttgt
     2341 tttaccgcag ccacttgcac ctaccaaagc agtgacctct ccctgcttag ctttaaaact
     2401 tacaccattt aaaacttttg cgtctttatt gtaggcaaac tcaacatctt ttagatcaat
     2461 atcaaatttt tttaagctat agtcatcgcc ttcttgtaaa tcttgatttt gaatttcttt
     2521 taatctttct attttgggcg ataaataaaa tatttccatc aagccctctt tagatgcatc
     2581 taaagagtct tttatcttca ta
//
LOCUS       DAVKGC010000152         2465 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVKGC010000152 DAVKGC010000000
VERSION     DAVKGC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.152"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..1365
                     /locus_tag="VW049_001957"
     CDS             <1..1365
                     /locus_tag="VW049_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEU9433008.1"
                     /translation="REYGVVVFYQSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQEN
                     MQLITEKENIIASLQDSKKNLIDLQWQIDYILSIYARQISKNNLLSTPHLVALEGWIE
                     ETRILYFIKVMDERFGHSIYIYESETLSDNQDEIPIKLTNHSLIEPFELLTEMYALPK
                     YYEKDPTPVLAPFYFTFFGMMVADLGYGLLLFLGIMLALKIFHLPSVTKKFLKFFNIL
                     GVAVAIWGGIYGSFFGYELPFHLITTTSDVMTILVVSVVFGFITVFVGLLASGLQKVR
                     MKKYAEAYNSGFAWCVILLGLLFIAVGMLMPDIRPLFVLGKWVSIFNAVGILIASIIQ
                     AKSLSGIGSGLFNLYNISSYIGDLVSFTRLMALGLSGASIASAFNLIVGLFPGTLAKL
                     TIGLVLFILLHAINIFLSLLSGYVHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKV
                     ITKN"
     gene            1381..1857
                     /locus_tag="VW049_001958"
     CDS             1381..1857
                     /locus_tag="VW049_001958"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEU9433009.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            1891..>2465
                     /locus_tag="VW049_001959"
     CDS             1891..>2465
                     /locus_tag="VW049_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433010.1"
                     /translation="MSNLENLRESVIEQAHEKGSMKLLDSKKKIDDEFEMQKSVIVKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIF"
BASE COUNT          794 a          331 c          486 g          854 t
ORIGIN      
        1 agagagtacg gtgttgttgt tttctatcag tccagttgct ctattgattt tgatgaatac
       61 ttatttgaac catttgatta ttctagaaag gaattaccga agcagcgagt agtagatttg
      121 gatcaagaaa acatgcagtt aataactgaa aaagagaata ttatcgcatc gttgcaagat
      181 tcaaagaaaa atttgataga tttacaatgg caaattgact atattttatc tatctatgct
      241 cgtcaaatct ctaagaataa ccttttgagc actccgcatc tagttgcatt agaaggatgg
      301 atagaagaaa ctcgtatttt atattttata aaagttatgg atgagcgttt tggacattct
      361 atttatattt atgaatcgga aacattgtcg gataatcaag atgaaatacc tatcaaatta
      421 acgaatcact ctttaattga accatttgaa ttattgacag aaatgtatgc tctgcccaaa
      481 tattatgaga aagatcctac acctgtatta gcaccatttt actttacatt ttttggaatg
      541 atggttgctg atttaggcta tggtttacta ttgtttttag gaataatgtt agcattaaaa
      601 atttttcatc taccttcagt aactaagaaa tttttaaaat tctttaatat attaggggta
      661 gccgttgcaa tttggggtgg aatctatggc tcattttttg gatatgagtt gccatttcat
      721 ctgataacta caacctcgga tgtcatgact atattagtag tgtcagttgt gtttggattt
      781 attacagtat ttgtaggttt gttagcttca ggactacaaa aagtaagaat gaagaaatat
      841 gcagaagcat ataattcagg atttgcgtgg tgtgttattc tgcttggctt gttatttatt
      901 gctgtcggaa tgttgatgcc tgatattaga ccgttatttg tattagggaa atgggtatct
      961 atttttaatg ctgtggggat tttgattgct tctattattc aagccaaaag cttgtcaggt
     1021 attggctcag gattgtttaa tctatataac atttcatctt atataggtga tttagttagt
     1081 ttcactcgat tgatggcatt aggattatct ggagcaagta tagcatcagc tttcaattta
     1141 attgttggtt tgtttccggg gacattggct aaactgacaa ttggattagt attattcatt
     1201 cttttacatg cgatcaatat ttttctatcg ttactatcag gatatgttca tggagcgcgt
     1261 ctgatatttg ttgaattttt tggtaagttt tatgagggtg gaggaaaacc atttcaacct
     1321 ttgaaggctt ctgagaaata tattaaggtt attacaaaga attaatggag gatatatata
     1381 atggaacatt tagcaactta tttttcaacc tatggaggag ctttcttcgc tgcattggga
     1441 attgtattgg cggttggatt aagcggtatg gggtctgctt atggagttgg taaggctggg
     1501 caatctgccg cagctttact gaaagaacag ccagaaaaat ttgcctcagc tttgatattg
     1561 caactattgc ccggaacaca aggattatat ggttttgtta ttggaatttt gatttggttg
     1621 caattaactc cagaacttcc tttggaaaaa ggcgttgctt atttctttgt agctcttcca
     1681 attgctattg taggatactt ttcggctaag catcaaggaa atgtagcagt agcgggaatg
     1741 caaatcttgg ctaaaagacc aaaagaattc atgaagggag caattttggc tgccatggta
     1801 gaaacctatg caattcttgc ctttgtcgta tcattcattt tgacccttcg tgtataagaa
     1861 attaatttgc tattcaaagg aggtgtctaa atgagcaatt tagaaaactt acgagaatct
     1921 gttattgaac aagctcatga aaaagggagt atgaaattat tggattccaa aaagaagatt
     1981 gatgatgaat ttgaaatgca aaagtcggtc attgtaaaga aaaaagaagc tgaacatgaa
     2041 cgaaagttaa aagaattaca acagaaatat caaataattt ttcaacaatt aaaaaataag
     2101 gaacgccaat caacgttagt atcaaaacag aaaatattaa aagaactttt tcaatctgct
     2161 ttaatagaaa tggaatcttg gagtgcagat aaagaaatgg agttcatcta tcgaattctg
     2221 gaacgatatt cacaacaaga ggtcatagta acttttgggg agcggacttt agctaaattc
     2281 aatttggaac aattagagaa attgaaattc tcttttccaa atcatttatt tagtgaacag
     2341 cctatctcaa atgaatcagg cttacttatt tcaataggta aaattgatga taactatttg
     2401 tataaaacgt taattggatc gatttctaag gaagaaagtt caagtatcgc aaatcaaatt
     2461 tttat
//
LOCUS       DAVKGC010000153         2442 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVKGC010000153 DAVKGC010000000
VERSION     DAVKGC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.153"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..268
                     /locus_tag="VW049_001960"
     CDS             <1..268
                     /locus_tag="VW049_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9433011.1"
                     /translation="THKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDIAFYDSK
                     DYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            270..>2442
                     /locus_tag="VW049_001961"
     CDS             270..>2442
                     /locus_tag="VW049_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU9433012.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTSISEVQHKSGNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVTRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPAPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIREHGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTQVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQISIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMK"
BASE COUNT          846 a          380 c          478 g          738 t
ORIGIN      
        1 aactcataag aaatatttag atattcatat tgttgttgaa aatacagaaa aaatggcagt
       61 aacttcccca gttcgtgctc agtcacgtgt accttttagc gaagagaagg atattgcttt
      121 ttacgatagc aaagactatc aaattgttga attgcttcca gggaatatgt tggtgacttt
      181 tgaagaagat cttcaccaac caaagattca ttgcaatgat gaaactgtta aaaaacttgt
      241 tatcaaagtt ttaaacgagg aaaaataaaa tgaaaaaaaa tattaaacaa tatgtaacct
      301 taggtactgt agtggtatta tcagcatttg ttgctaactc agttgcagct caggagactg
      361 aaacttctga agtatcaaca ccagagttgg tgcaacctgt tgcaccaacg acttcgattt
      421 cggaagtaca acataaatcg ggtaactctt cggaagttac tgtacaacct cgaacagttg
      481 aaactactgt taaggatcca tcttctacag cggaagaaac tcctgtctta gaaaaaaata
      541 atgttacttt aacagggggc ggagaaaatg ttactaatga gttaaaggat aaatttacta
      601 gcggtgactt tactgtagtg attaagtaca atcagtctag tgagaaaggt ttacaagctc
      661 tgtttggaat atctaattct aaacccggtc aacaaaatag ttatgtagat gtgttcctta
      721 gagacaatgg tgaattggga atggaagcgc gtgatacttc ttccaataaa aatcacctag
      781 taaccagacc tgcttcagtt tggggtaagt acaaacaaga ggccgtgacg aacactgttg
      841 cagtagtagc agattcagtc aaaaaaacgt attctttata tgcaaatggt acaaaagtag
      901 tagaaaagaa agtggataac ttcctaaaca tcaaggatat taaaggtatt gattactata
      961 tgcttggggg agtgaaacgt gcaggaaaaa cggcgtttgg ttttaacgga acactagaaa
     1021 atatcaaatt ctttaatagt gcattggatg aagaaactgt taaaaagatg acaacaaacg
     1081 ctgttactgg acatttaatt tatacggcta atgatacaac aggttctaac tatttccgta
     1141 ttccagttct gtatactttt agcaatggtc gggttttttc aagcattgac gctcgttacg
     1201 gtggaactca tgatttcttg aataaaatta atattgctac aagttatagt gatgataatg
     1261 gtaagacatg gactaaacca aaattaacat tagcattcga tgattttgcg ccagcaccat
     1321 tagaatggcc tcgtgatgtt ggtggacgtg acttacaaat cagcggtggt gcaacctata
     1381 ttgactctgt tattgttgaa aaaaataaca aacaagtact catgtttgct gatgttatgc
     1441 ctgctggagt aagttttaga gaagctacta gaaaagattc aggttataaa caaattgatg
     1501 gtaattatta ccttaaatta aagaaacaag gtgatactga ttataattat actattcgtg
     1561 agcatggtac tgtatacgac gatcgtacca acagaccaac tgaattttca gtagataaaa
     1621 atttcggtat taaacaaaat ggtaattatt tgacggtaga gcaatattcg gtttcatttg
     1681 aaaataataa aaagactgaa tatcgtaatg ggactcaagt ccatatgaat atcttctaca
     1741 aagatgcatt attcaaagta gtgccaacga actatattgc atatatttct agcaatgatc
     1801 atggcgaatc ttggtctgca ccaactttat tacctcctat aatgggactt aatcggaatg
     1861 cgccttattt gggtcctgga cgtggaatca ttgaaagctc aactggacgt attcttattc
     1921 cgtcttacac tggtaaagag tctgcattca tttatagtga cgataatgga gcgtcttgga
     1981 aagttaaagt tgtgccactt ccttctagtt ggtcagcaga agcacaattt gtagaattga
     2041 gtccaggagt aattcaagca tacatgcgta caaataatgg taaaattgca tatttaacaa
     2101 gtaaagatgc aggtactact tggagtgcgc cggaatattt gaaatttgtt tcaaatccaa
     2161 gttatggaac acaattatca atcatcaatt atagtcaatt gattgatggt aaaaaggctg
     2221 tcattttaag tactccaaac tccacaaatg gtcgtaaaca cggacaaatt tcgattggtc
     2281 taattaatga tgataataca attgattggc gttatcatca cgatgttgat tatagtaact
     2341 atggatactc atattcaaca ttgacagagt taccaaatca tgaaattgga ttgatgtttg
     2401 aaaaatttga ttcatggtct cgtaatgaac ttcatatgaa aa
//
LOCUS       DAVKGC010000154         2425 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVKGC010000154 DAVKGC010000000
VERSION     DAVKGC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.154"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            115..1122
                     /locus_tag="VW049_001962"
     CDS             115..1122
                     /locus_tag="VW049_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9433013.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIVDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1125..1325
                     /locus_tag="VW049_001963"
     CDS             1125..1325
                     /locus_tag="VW049_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9433014.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1477..1659
                     /gene="rpmF"
                     /locus_tag="VW049_001964"
     CDS             1477..1659
                     /gene="rpmF"
                     /locus_tag="VW049_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9433015.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1675..1824
                     /gene="rpmG"
                     /locus_tag="VW049_001965"
     CDS             1675..1824
                     /gene="rpmG"
                     /locus_tag="VW049_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9433016.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(2316..>2425)
                     /locus_tag="VW049_001966"
     CDS             complement(2316..>2425)
                     /locus_tag="VW049_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9433017.1"
                     /translation="TVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT          800 a          461 c          446 g          718 t
ORIGIN      
        1 ccttttaaaa gaaattattg ggctgaaact ttttagtgat tattttcaac tttctattga
       61 cttttaaaaa caaagtgata tcatttagat atcaaataat gggaggtcat tcttatgact
      121 gaaaaactaa tcaattcaaa accaaatggt gtattcgcat tgattctcat tgagttgaca
      181 atcgtacttg gtatctttat atttataatg ggggttggtt cggaaaacat ttttggaatt
      241 attatcggac ctttactaat cgtaattgca ggtctagctc atgctggttt aaaagttgtc
      301 aaacctcagg aagctctggt tctgacactc tttggtaatt atacaggtac catcaaagaa
      361 cctggttttt actttgtcaa tcccttcagc gtagcagtca accctgcaaa ccacactcga
      421 cttggacaaa gtggtgatgt tagcacaaaa tctccttttt taggagctaa atcatcaaat
      481 gacaatgatg taaatcttga aattggcaag aaacacattt ccctcaaagt catgaccttg
      541 agcaattctc gtcaaaaaat caatgattgc ttaggaaacc ctgtagaaat cggtatcgcg
      601 gtaacttgga gagttgtcga taccgctaag gcagtcttca acgttgataa ctacaaagaa
      661 tatctttcat tgcaatgtga tagcgccctc cgtaatattg tccgcatcta tccttacgat
      721 gtgtctccta atgtggatac tacgggtgac gggcaagcag atgaaggtag tctccgtggc
      781 tctagcgaaa ttgttgctaa ccgtattcgt gaagaaatcc aaagtcgtgt tgaggatgct
      841 ggcttggaaa tccttgaagc acgtatcact tacctagctt atgctccaga aattgctgcc
      901 gttatgcttc aacgccaaca agcatctgcc attgttgatg cacggaagat gattgtagat
      961 ggtgctgtag gaatggttga aatggcacta gaacggctca atgaagggga attggtagaa
     1021 cttgacgaag aacgaaaagc tgccatggtt tcaaatctcc ttgtcgttct ttgtggcaat
     1081 catgatgcac aaccaattgt caacacagga agtctttact aaagatggct gaagctaaaa
     1141 aaaagcagat cccccttcga ctctcaacaa agttatacgc tgcactcgca tcatgggcag
     1201 aagacgactt tcgttcagtc aatgggcaaa tcgaatatct cctcacagag tgtgtcaagc
     1261 aacggaagaa agacggaaaa tacgtatcag aaaccattga cgaaccattt gagattgata
     1321 tttaacatct tctcctgtct gacaagtcgg acaggagaat tttttcatct ttttttgtca
     1381 agtaacttta ctttacaaaa aaaatgtgtt atcctagtat ggttgatgaa aatcagtaga
     1441 ttgaatcgaa tataaatacc taaaggagaa atcaaaatgg cagtacctgc acgtcgcact
     1501 tcaaaagcga agaaaaacaa acgtcgtaca cactacaaag taacagctcc atctgtaaac
     1561 tttgacgaaa ctactggaga ttactcacgt tctcaccgtg tatcacttaa aggatactac
     1621 aaaggacgta aaatcgctaa agctgcatca gctgaataat agaagggaga taccatgcgc
     1681 gtaaatatta cacttgaaca caaagaatct ggtgaacgct tgtaccttac ttctaaaaac
     1741 aaacgtaaca ctccagaccg ccttcaattg aagaaatact caccaaaact tcgcaaacac
     1801 gttgtgttta cagaagtgaa gtaagcattc aatacgaatc aatacagaag aaaaacgctt
     1861 gatcagcgtt ttttcttttt gttaatttca ctatattgca agggaaatca ttcgaatgac
     1921 tacctttttc actacctttt ttgaaataag aatacggatt cggacgatta aaagtaagag
     1981 aaactcaaat gtaagttggg gttccttttt tgtatatttt tcaaataacg cctctacact
     2041 atttgtagca aattcaccaa ctacagttgt atcttagtta aaataagtta gaatatgtaa
     2101 gtgagtacca gatataccaa gacatcgtca ccatctaagg tatattcaaa atacaaaagt
     2161 tgaccaacta gatttctgaa tatccttata tatccattct taaaattggt ttaaatagcg
     2221 tagtctttta aactagtttt gagaatccaa aaaatcttcc tacatatgta agaagatttt
     2281 ttagttcaga atgattagat ttagctaatg gatacctatc ctacccactc accgttagca
     2341 ttgactcgat agccatcggg tgtagtcgtg tttactaata aatctcctga attataggta
     2401 tagtaccatt taccagaaac tgtaa
//
LOCUS       DAVKGC010000155         2405 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVKGC010000155 DAVKGC010000000
VERSION     DAVKGC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.155"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..154
                     /locus_tag="VW049_001967"
     CDS             <1..154
                     /locus_tag="VW049_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9433018.1"
                     /translation="KKILLPLPPLALQNEFADFVALVDKSQLAIQKSLEELETLKKSL
                     MQEYFG"
     gene            214..450
                     /locus_tag="VW049_001968"
     CDS             214..450
                     /locus_tag="VW049_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9433019.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            447..860
                     /locus_tag="VW049_001969"
     CDS             447..860
                     /locus_tag="VW049_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9433020.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            978..1943
                     /locus_tag="VW049_001970"
     CDS             978..1943
                     /locus_tag="VW049_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9433021.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            1909..>2405
                     /locus_tag="VW049_001971"
     CDS             1909..>2405
                     /locus_tag="VW049_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9433022.1"
                     /translation="MSSIHTKNSSLKSKSRFNEMFGENKIFESIDNLFDIIDGDRGKN
                     YPKSDELFSEEYCLFLNTKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRG
                     TVGNVAYYDELIKYKHLRINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQ
                     LPITK"
BASE COUNT          885 a          362 c          454 g          704 t
ORIGIN      
        1 aaaaaaaata cttctccctc tcccccctct agccctccaa aatgagttcg cagactttgt
       61 agccctggtc gacaaatcac aattggcaat ccaaaaatct ctggaagaac ttgaaacttt
      121 gaagaaatct ctgatgcagg agtattttgg ctgatattct gccattgtaa ttacggtaat
      181 gatttgttat aatacttcaa aggaggaaat cagatggtag taaaaacaag aaaacaagga
      241 aattcaatca ccattacgat tccaagtgaa tttaatattc caagtggtgt taaatacgaa
      301 gcgaaattgt taccaagtgg tgagattatc tttactcctg aagaattggg gcagcaggtt
      361 tcttatgtat ctgatgatgc ctttgactta aatttagata aaatatttga cgaatacgac
      421 gatgttttca aagctttggt ggaaaaatga caatctatct gacagaaaag caaattgaaa
      481 aaataaatgc tttagcaatt caacggtatt ctccaaatga gaaaattcaa acagttagtt
      541 cttctgcctt aaatatgatt gtgaacttac cagaacaatt tgtctttggg aagtctcttt
      601 atccaacaat ttttgataaa gcaacgatac tatttgtcca attgataaag aagcatgttt
      661 ttgctaatgc taataaaaga actgctttct tcgttttggt caaattttta caattaaacg
      721 gctatcgttt ttctgtaacg gtagaagaag cagtaaaaat gtgtgtaacc atcgcagtag
      781 aagctttaac tgatgaaaaa atgacaagct actccaaatg ggtttctgaa cattctgtta
      841 gagaaaaggt caaaaagtaa cctagtatgc tggatttgaa tgagcacaag aaaataaatg
      901 aacagacaat attagaattc tgtaatgcag aaactgatat tgtctctttt tattgatgaa
      961 taagaaagtg agaaattatg gaatcaaaag ttacaattat catgcaagaa atgttacctc
     1021 ttttaaataa tgaacaatta ctagcgttga gagagagttt agaacatcat ctagtagacg
     1081 gaaaaaagca gcagaagtat tcgaataata acctgttgca actatttatt accgccaagc
     1141 aggtagaggg ctgtagctca aaaacaattc gttattacca gaggacgatt gaaaacttgt
     1201 ttaatgctat taaagagtct gtgacacaac tcacaacaga tgatttaagg agttatttag
     1261 caaattacca gtctgaaaag gattgtagta aggcaaattt agacaatatt aggcgtatat
     1321 tgtcttcttt ttttgcttgg cttgagcaag aggaatatat cattaaaaat cccattcgac
     1381 ggataaagaa aattaagact gagcaaaatg tgaaggaaac ttatactgat gaacatttgg
     1441 aaattatgcg tgataactgt gaaaatttga gagatttggc aataatagac ctactagcat
     1501 cgacaggtat gcgtgtaggg gagcttgtac agttgaatcg ttcagatatt gattttgaaa
     1561 acagagagtg tgttgtcttt ggtaaaggaa agaaggagag accagtatat tttgacgctc
     1621 ggacgaaaat tcatttaaga aattatctta acgacagaaa agatagtcac cctgctcttt
     1681 ttgtaacgct agttggaaaa gcccagagac ttggaattgc tggtgtagag attcgcttaa
     1741 gaaagttagg agacaaactc ggcatacaaa aggttcaccc acataagttc agaagaactt
     1801 tagcgactaa ggcaattgat aaaggtatgc ctatcgaaca agtccaaaaa ctgctaggtc
     1861 atagcaagat tgacacaacc ctggcctatg ccatggtcaa tcaaaataat gtcaagcatt
     1921 cacaccaaaa attcatctct taaaagcaaa tcccgattta acgagatgtt tggggaaaat
     1981 aaaatatttg aaagcattga taacttattt gatattatag atggtgatag gggcaaaaat
     2041 tatcctaaat cagatgagtt gtttagtgag gagtactgtt tatttttaaa tacaaagaat
     2101 gttactaaaa acggattttc attcgataca aagcaattta tcactaaaac aaaggataaa
     2161 ttacttcgaa aaggcaaact tgagcgttat gatatagtct tgacaacaag aggtactgtt
     2221 ggaaatgtag cgtactacga tgaattaata aaatataaac atttacgtat aaattcaggt
     2281 atggtaatat tacgtcccaa gacaccaaat ctaaatcaga aatttattat ccatgtttta
     2341 aggaataata attatagtcg agtgatatca ggaagtgctc agcctcagtt accaattaca
     2401 aaatt
//
LOCUS       DAVKGC010000156         2316 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVKGC010000156 DAVKGC010000000
VERSION     DAVKGC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.156"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            231..605
                     /locus_tag="VW049_001972"
     CDS             231..605
                     /locus_tag="VW049_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU9433023.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            813..1715
                     /locus_tag="VW049_001973"
     CDS             813..1715
                     /locus_tag="VW049_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433024.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPLDFVVSTSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTKTLKDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWSNKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            1771..2010
                     /locus_tag="VW049_001974"
     CDS             1771..2010
                     /locus_tag="VW049_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9433025.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAAAGVLATA
                     AGKIAYCIGYGVLNRGCDINGNPYPWDGFISATVR"
     gene            2007..>2316
                     /locus_tag="VW049_001975"
     CDS             2007..>2316
                     /locus_tag="VW049_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433026.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLISFPILINKFFPESFLTYTVLISVFILELAIFHL
                     I"
BASE COUNT          763 a          304 c          424 g          825 t
ORIGIN      
        1 aaaaaaaata aattttgaaa tagtattggt gagttcgcaa tcatttattg ctagtggttt
       61 agaactgtta agtttcaaac aataattgat tttaaacctt aggtaaccta taaaaacaaa
      121 taaggtagag aaaggatatc ttatctaggt agctaagtga taaaaacaca gtcgtctatc
      181 acttgttagg aaactatcct aactatgctt aataaaaaag gagaaaataa atggaactcg
      241 tattaccaaa taattatgtt gctcttgagc aagaagagat gatgtatctt gatgggggat
      301 tttctattct gagatggcct gttgcaacag ccattaatat agcttttaat ggtgttttag
      361 gtggaggagc aatcagtcta gttagaaatt atattcgtaa ttatggtttg cggcgagtta
      421 caagcgcaat tgctggagca gctgcaagat atgttggggt acgagttgca aatagagtgg
      481 caggatttgc actgtctgct attaatggat ttgcagcttg gatgtcaatt ggcgatgcta
      541 ttacaacaat ctgggccaac aatgatgtaa ataggagaga cccaaattta aacgccttgt
      601 ggtaactaga tatgggtatt aaaatggaaa aaatttttgt tattattttt tttgtatgtc
      661 tatttatatc atccataact tttttagcct atgattttgt tagcgaagaa ataaaaaaat
      721 tgattatttg gatgaatgtc gtttttttga ttttaatcat agcaatgata atttatccaa
      781 aattaagaaa atgataagga cactgtaaaa ttatgatacg taaactaatc atttttttat
      841 taatgctccc tctttttgtc ttgtggctag agttgcattt gttagtgagt aacttgcaat
      901 tgaatctaga aattcccctt gattttgtgg ttagtacgag tcttgctttc tgtagtttga
      961 ttttatcaaa aatagtttta gacatactat acgcattgaa agatttgtac aagaaagaag
     1021 ctcttattac gatttttcct tttattttta taggcaggaa gaaagtaaat gtgaggttct
     1081 cgccctattt ttcattccat cgtaaaagtt tgtcgcctga tgatttaaga tctaggatta
     1141 tatggagttt tattcttgaa attgctatta ttctagtatt tattttaaaa attccttttg
     1201 caatcatcat gcttactaca atatttttct ggactataat ggatatcaat catctcgttt
     1261 ttaataagac ggaatttctt tttaatcaaa ataaatggga aaaagaagat tcgtttgaaa
     1321 gcgatttgac gaaaacgtta aaggacaaaa tacaaaaatc agagttaagc tattctgact
     1381 taatgtccct tcttctctat gatgcgatga accaatccac ctttttaacg gatagtgaac
     1441 tttttgagga tattttgaaa aaaatcgaag attctcataa cacccttctg tgtacaggtc
     1501 ttgtcgagtt actgctgtat gagatttcta ttagtaacaa taacaactgg tccaacaagg
     1561 ttgataaaat tagaatacag ctcatcagaa tcaatcaatt agattttttc tattatacaa
     1621 gttggttaag gcaaaatttt gatttctgta tgaatagaga gtatcataaa atgaaatctc
     1681 ggaaattatt actgagtaat aaaaagatag tttaagttcg tcctatttgt aaaacaaatg
     1741 aaacaatttc aactaaggag gagaaaacaa atggaacttg tattaccaaa taattatgtt
     1801 gtgattgatg aagaagtaat gatgtatctt gatgggggag cttatttaag caagcgtgct
     1861 tgtcaaggaa tttgcgcagc agcaggtgta ttggctacag cagcagggaa aatagcttac
     1921 tgtattggat atggtgttct taatagaggt tgtgatatta acgggaaccc ttatccttgg
     1981 gatggattca tatctgcaac agtaagatga gtaatgtaga taagattcga aaaatacaca
     2041 tcatcgtgtg ttgggtgtat atatttttat cattcagggc gattataaat gatacggagt
     2101 attttttgct tattttttta gcttttatct actcaatagt atcactacct ctatattctg
     2161 tgaaaaataa aatagtatct atctgtctag ccataaattc gattctgtta atcagtttcc
     2221 ccattttaat caataagttt ttcccagaaa gttttttgac ttataccgta ttaataagtg
     2281 tttttatcct agagttagca atctttcatt taattg
//
LOCUS       DAVKGC010000157         2242 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVKGC010000157 DAVKGC010000000
VERSION     DAVKGC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.157"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            104..1627
                     /locus_tag="VW049_001976"
     CDS             104..1627
                     /locus_tag="VW049_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9433027.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGIILLALTIIGGLILGAMMGE
                     QEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFKSFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVHGNINNFKNYNIEFENVSFAYNDKAVIEN
                     LSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSIKIGGIAISEYSDEALIKAI
                     SFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGCDLILDKFPERENTIIGSKG
                     VYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEFELQKAFKNLMKDKTVIMIA
                     HRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSLQEMFNSANEWRVSNERVL"
     gene            1593..>2242
                     /locus_tag="VW049_001977"
     CDS             1593..>2242
                     /locus_tag="VW049_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9433028.1"
                     /translation="MNGGFQMKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAI
                     LLMIFAQEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTA
                     ENLSKLPLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAG
                     NVKMGLAVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKA"
BASE COUNT          798 a          306 c          419 g          719 t
ORIGIN      
        1 agataagtta ataattaatt caaatttatc cggtgcagag agtatagcat taaaatctgt
       61 tattacacta acaagtggag cgatatttta ttttgtctca ggaatgtttt cacacatctt
      121 gggatttagg cttgaaacaa atttaagaaa aaggggaatt gatggcttag agaaagcaag
      181 ttttaggttc tttgacttaa atccatctgg tcaaataaga aagatcatag atgataatgc
      241 agcacaaact catcaggtgg tagcacacat gattcccgat agttctcagg caataatcac
      301 acccgtactt gtacttgcac ttggctttat agtaagtata agagttggca taattttgct
      361 tgctcttact ataattggcg gcttaatttt aggggcaatg atgggcgagc aagaatttat
      421 gaagatatac caagaatccc tatctaaact aagtgctgaa actgttgagt acgtgagagg
      481 aatgcaagtt gtaaaaatat ttaaagcaaa tgtagagtct tttaaaagct tttataaggc
      541 gataaaagat tactcaaagt atgcttatga ttattcccta tcttgtaaaa ggccttatgt
      601 tttgtatcaa tggttatttt ttggactgat tgcaatttta attattccta tagtttattt
      661 tatgactagc ttagctagcg caaaggtgat tttacttgag cttatcatga ttttattttt
      721 atcaggagtt ctctttgttt cattcatgag aatgatgtgg tactccatgt atatttctca
      781 aggaaattat gcagtagata ctttagaggc gctttacgaa gatatgcaaa aagacaaatt
      841 agtgcatggt aatatcaata attttaaaaa ctataatata gaatttgaga atgttagctt
      901 tgcttataat gataaagctg tcattgaaaa tttatccttt aatttagaag aaggaaagtc
      961 ctacgcactt gtcggttcat ctggatcagg caaatcaaca gtagcaaaac ttatatcagg
     1021 tttttataat gttaataaag gaagcataaa gataggcggg atagcaataa gtgaatattc
     1081 tgacgaagcc ttaattaaag ccatttcctt tgtttttcaa gattcaaaat tattcaagaa
     1141 gagcatttat gataatgtag ctttagctaa taaagatgcg acgaaagatg acgttatgag
     1201 agccttaaaa ttagcaggat gcgatttaat attagacaaa ttcccagaaa gagaaaatac
     1261 aatcataggc tcaaaaggtg tttatttatc cggtggagaa aaacaaagaa ttgcaattgc
     1321 tagagcaatt ttaaaggatt ccaaaattat tattatggat gaagcatcag catctattga
     1381 cccagataac gagtttgaat tgcaaaaagc ttttaaaaat cttatgaagg ataaaacagt
     1441 tatcatgatt gcacacaggc tatctacaat taaagacctt gatgaaatta ttgtcatgga
     1501 tagtggaaaa attatagaaa gagggtctga caaagaatta atgtcaaaag atacaaggta
     1561 taagagcctg caagagatgt ttaacagtgc gaatgaatgg agggtttcaa atgaaagagt
     1621 tttataaaaa aagatttgct cttacagatg gaggagcaag aaatttaagt aaagcaacac
     1681 tggcttcatt tttcgtttat tgtataaaca tgcttcctgc catattactt atgatttttg
     1741 ctcaggaagt tttggaaaat atgggcaaaa gcaatggctt ttatatagta ttctcagttt
     1801 tgactttgat agcaatgtat attttgcttt ctatcgaata cgataaatta tataacacaa
     1861 cctatcaaga aagtgcagat ttaagaataa ggacagcgga gaatttatca aaattacctc
     1921 tatcttactt ttctaaacat gacatttccg acatttcaca aacaatcatg gctgatattg
     1981 aaggcataga gcatgcaatg agccactcaa taccaaaggt gggcggcatg gtactgtttt
     2041 tcccattaat atctgtaatg atgctagcgg gcaatgtcaa gatgggttta gctgtaatta
     2101 tcccatctat tttaagcttt atatttatac ctttatctaa aaaatatcag gttaatggac
     2161 agaatagata ttatgatgtc ttaagaaaaa actcagaaag ctttcaagaa aatatcgaaa
     2221 tgcaaatgga gattaaagca ta
//
LOCUS       DAVKGC010000158         2201 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVKGC010000158 DAVKGC010000000
VERSION     DAVKGC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.158"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(330..1223)
                     /locus_tag="VW049_001978"
     CDS             complement(330..1223)
                     /locus_tag="VW049_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9433029.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(1977..2192)
                     /gene="blpU"
                     /locus_tag="VW049_001979"
     CDS             complement(1977..2192)
                     /gene="blpU"
                     /locus_tag="VW049_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU9433030.1"
                     /translation="MSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQLGIKT
                     RTWQGAATGAVGGAILGGVAYAATCWW"
BASE COUNT          730 a          470 c          243 g          758 t
ORIGIN      
        1 tttaactttt atacgattac taccttcttt ttagtttaca cttcctacat cataaataaa
       61 catactcaac cctcacaatc tccaaataac tcttctatat cattgaaaat tgagtttcat
      121 cctctatact aatgtaggac attcctttaa tccaatataa gaataccttg ttccattaat
      181 ctctacccga tattatagat agaaccaaat tcctatactt aaaaggacaa ataacgttcc
      241 aaccataata aacctccttt ctttttgaga acgttttctt tataggttta gtatagcaaa
      301 aacatgaaaa aaacaacatg acttagaagt caggttgttg ggaaatctat ctgtttttct
      361 gctaaaattc tctgaactaa tacctgatcg tctagcaatt ctgctagaga aatcgccttt
      421 tcatagaaac tttctgcctc taaaatattc ttgtctacaa ataaagcata ttttgccttt
      481 agcaaaaata gatgaggtcg gtaaaaaaca tgtcttgttt tagcaataat tttctcaaat
      541 gtttgaatat atatcagact atcttctgtc tgaatttgga taaataggct cagaagaact
      601 ttttccattt gaactaggct agccttatct tcatattctg aacatagcaa tactttttga
      661 gctaactcct caaaatgttc tttatcttct aatcccatag cacaacaaaa gaaatacaat
      721 tcaataatca acaaatcatt tacagaaaaa tatttctttt ttaataactg ctggaaatat
      781 tcttccacta accctaaacc gtaggtcaca tctgaactac cataaatatc aaaacgagct
      841 tgtgtcacat ccacaattaa ctgctcttcc tcaggcaaat tctcataata gttttcatat
      901 atttcctcaa gtatagcttc ttttcgttcg attcttccct tatcagcgta ggtttgagaa
      961 tgaatcagct catctttgag ttttaggaat ccttttggaa tctctataac cttaacatcc
     1021 acaatattct gtaatgatac tccaagttta tccgcaatca ataataattt aggaagtgtt
     1081 gctaaagctt gcccattttc aatacgtgct agctgacgaa tcgtcaactc tgattcatca
     1141 ccacaaatat ctatctgagt ttttccacaa gatcgcctaa tagttttgat tttataacca
     1201 atttcacgtt tcaactcatc catagtaata ctccttaagt tgattacaaa gatttctttt
     1261 tcttcctaat tcttttgtca atcatatata aaacgataag cgaaaacagc ggtagaacaa
     1321 tataactctt ttcactcatt atcagctgaa atcccagggt tgctagtatc ttaaagatga
     1381 aaatattcat caaaaataga aaaacaaata ctaaaaaata ttttaaaacc ttcttcattt
     1441 cttttcttca actttcctaa tttctacacc tattttatcg aaaatatata taaaactcca
     1501 aataaacact tcaactgcac tttttttggc aaaaaataga cagaacaaac atcctaccta
     1561 ctaaaaatat gatatgaaag gaaaatgatt ttttactgac accctaggca agcttgggac
     1621 aaataaatca ttgatagcaa catttacgac atcctattta aagttccttc aatttctaat
     1681 gtccccgcta tttctaagcc acctctggta tgatctattc ctagtcagaa tagataacac
     1741 cgttctaaat tacgattcat agttactcct tttttgactc gttactataa gactcctatt
     1801 tacatgcgca aaataatttt gattcagatt catcatttat agttttgatt attttttaac
     1861 agatttttct gttttcttct ccgaagactt taaaaatttt tttctaatta aatcatccat
     1921 ataaggacca aatataccaa ctactaaacc aataataaaa cttttaaaat ccataattac
     1981 caccaacatg tcgctgcata ggccacacct ccaagtatag ctcctcccac agcaccagtt
     2041 gctgcacctt gccatgttct tgttttaatt cctagctgaa gacctcgtgc tgctcctcct
     2101 ccaacacctg ctttggcaaa atctccccaa ttgcatccgc caccttcaac gcaagcaagc
     2161 atctcagtat ccataatttc aaattgtgac atcgtttttg t
//
LOCUS       DAVKGC010000159         2057 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVKGC010000159 DAVKGC010000000
VERSION     DAVKGC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.159"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(187..465)
                     /locus_tag="VW049_001980"
     CDS             complement(187..465)
                     /locus_tag="VW049_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433031.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKLTSCCIVAKDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            complement(450..689)
                     /locus_tag="VW049_001981"
     CDS             complement(450..689)
                     /locus_tag="VW049_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433032.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTNLHHLF
                     GLHKITRDFASHTIPAIQAGVFILEDFRNILCTMM"
     gene            complement(1825..>2057)
                     /locus_tag="VW049_001982"
     CDS             complement(1825..>2057)
                     /locus_tag="VW049_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433033.1"
                     /translation="VDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQ
                     KAGYLFSVLLLFSNFSVPFIKAKEYELFKNSK"
BASE COUNT          727 a          382 c          268 g          680 t
ORIGIN      
        1 ttacaaattt ctatatcata agcttctact ccaaaaagat aaagacacta cttttgccct
       61 tttcgattct ctttttaaac atagcatcat cttccttcat tcctataaaa acatttccga
      121 tgagagagct tgttttttaa tttcgttaaa caacaaaaaa cgcaagctta aggctgcgga
      181 caatctttat agccatttca tttctttcac atctgtttta cgaactttag catatttctt
      241 agcgcttgta aaatgtagag taactggttt aaacactccg cttttgtctc ttcgtaaacc
      301 taaaactgcg gatcttttat ttttatcctc aaaaaatatg acgtctaatt tcatagtatt
      361 tttagataaa tccttagcta cgatgcaaca acttgttagc ttagaataga agatatcgcc
      421 gataaactca tataaagata tcctttcgat tacatcattg tacataggat atttctaaaa
      481 tcttctaaaa taaaaacacc agcttgaata gcaggtatcg tatgactagc aaaatctcgt
      541 gtgattttgt gtaaaccaaa taaatgatgt aaattagtca agcaaaaatt cactttgaaa
      601 gtctctaaat acttataatt cgttgatact tgacatgttt ttccattgaa tctttcttga
      661 taatccaaaa tacaatcaag taatttcaac gacaagctcc atattttctc aaaataaaaa
      721 agcacggggc agttctcact actgcccaaa gtggttgatt agagtcggct tctctaactc
      781 cctatgttgt gcttattagg cttatcgcaa gggaacagtt tttagctctg ccaagccaca
      841 tagtgtgctt actactatgt gtccatgtca ccatgaattg ccgaacggtt taacgaaaaa
      901 gtatagtaga ttgaaacaag atgtgaacaa atcgattagg aaagtcaaat taatttctag
      961 aaatatttta acagtcataa cggactattc cagattcaat ctactatatt acttttaggt
     1021 ttatcccaac tcaattatga catctttttt tcaaaagtca atatatctca ctcttttcaa
     1081 cgacaagaaa gaggctgata atcgaccaac ctctgttttt agatacagtt caagtccaaa
     1141 ctattcttta tagaaaagtc cgataaattc gagcaatagt taacatactt acaaatataa
     1201 ctcccaaaat agataacact attttcacaa aactcagttc cgaacctatg acatatatat
     1261 gaaaaagagg ggctagtcct acacatatat acaataagta gccaacaagt tttatctcac
     1321 caattcctgc aaaaaaaatg tttcatgtaa cgattctcca tttactccat taaatcagtt
     1381 ttattttaga ctatcataaa acaatcacct cttcaataaa aaccattggc gaaatactat
     1441 tctgaagcat tgatagctca gatttctcag aatagagctg tcctaactat cgagcagctc
     1501 aaatacatat atatgagcaa ttctaagcct agaattgcta taaaataaat gttttcaggc
     1561 tattctaagt ctttgactgc ttaaaatttt acgttctgaa ggattctaaa gctagaatag
     1621 ctcaaaatca cgagaactaa ggtattcgaa acttcagagt aatacaatcc ccataggaat
     1681 tctccattct tattaagcat gattaaaatg taatcctaac aagtgatcct cgattgtgtt
     1741 tgtagcactt agttacctag ataagactat ttccctttat catatctgat tttaagccgt
     1801 tctaagattt aatccatatt tctattactt gctattttta aataattcgt attcctttgc
     1861 tttgataaat ggaactgaaa aattagaaaa aagaagtaaa accgaaaaca aatagcctgc
     1921 tttttgaatc actaaaagat atcctaaaaa taagtaagct ataacatcca atgcgaaaag
     1981 aaaaaatatt ctcctcttca ttaaagtact tgtgtcaaat aatctgatga ataaaaattt
     2041 tatcgtagta tctacac
//
LOCUS       DAVKGC010000160         2033 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVKGC010000160 DAVKGC010000000
VERSION     DAVKGC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.160"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            447..1922
                     /locus_tag="VW049_001983"
     CDS             447..1922
                     /locus_tag="VW049_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9433034.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          606 a          375 c          454 g          598 t
ORIGIN      
        1 tgttttttat caaaaaatac tttacaagtt cttaaaaaca tgatatagta ataaagctta
       61 gaaaatgaga tgatgttttc tagcaaatat aaacccgagt aaaaaatgcc tacggacagg
      121 cagggttgaa tgccgaagcg tggttgaaaa gccacattat tgatagggtt aaaagcctac
      181 ttttataagt tgatgttagg acacttgtcc taattcataa atttttagtg tggtgaaagc
      241 acacgtcatc ttgtgaaacg atcaataaag tacgtaatat ttgctactag agagttagga
      301 aacatcggga acagacatac tcaacagaaa ccaaaataaa cacgtcagaa gattgcagag
      361 caggtgaaaa cctgctcttt tttcatgagt caacctttag ttccttagtt ttcataaggt
      421 cctaaaaata ttgaaaggag tatgttttga aagagttaga tcaaaaccaa gccccaattt
      481 atgaggcctt ggtgaagtta cgcaagaaaa ggattgttcc ctttgatgtt ccaggtcaca
      541 agcgtggacg gggaaatcca gaacttgtcg aactcttagg agaaaaatgt gtaggcattg
      601 atgtcaattc gatgaaacct ttggataatt taggccatcc tatttcgatt attcgtgatg
      661 cagaggagct ggctgcagat gcttttggag ctagccatgc ctttctaatg attggtggaa
      721 caacttcatc ggtgcagact atgattctgg caacctgcaa ggcaggagat aagattattc
      781 tgccacgaaa tgtccataaa tctgctatca atgcgttggt tctatgtggt gccattccca
      841 tctatatcga gatgagtgta gatcctaaga ttggtatcgc tttaggtctt gaaaatgacc
      901 gagtagcaca ggccataaag gaccatccag atgctaaggc tatcctaatc aacaatccta
      961 cttactacgg catctgttca gacctaaagg ggttgacaga aatggctcat gaagctggca
     1021 tgatggtttt agtagatgaa gcccacggag cgcatttgca tttcactgat aaacttccaa
     1081 tttctgctat ggatgcaggg gctgatatgg cagcagtttc catgcataag tctggtggga
     1141 gtttgaccca aagctccatt ttacttatcg gggagcagat gaattctgaa tacgttcgtc
     1201 agataattaa cctgacccag tctacatctg cctcttactt gttgatggct agtttggata
     1261 tttcacgtcg caacttggcc cttcgtggta aagagtcgtt tgagaaagtc attgagctat
     1321 ctgagtatgc ccgccgtgaa atcaatgcta tcggtggcta ctatgcctac tcaaaagagt
     1381 taatagacgg tgtttcggtt tgcgattttg acgtaactaa gctgtcagtt tacactcagg
     1441 gtattggctt aacaggtatc gaggtttatg acctcttgcg agacgaatac gacattcaga
     1501 tcgagtttgg tgatatcggc aatatcttgg cctatatttc catcggcgac cgcatccaag
     1561 acatcgagcg cttggttggt gctctggctg atattaagag actctattca agagatggaa
     1621 aagatttgat agcaggagaa tatattcagc ccgagttagt gctgtctccg caagaagcct
     1681 tctattcaga aagaaaaagt ttaactttgg atgattctgt tggacaggtc tgtggagaat
     1741 ttgttatgtg ttaccctcca ggtattccta tcttggctcc tggtgaacgc attacacgag
     1801 aaattgtcga ctatatccaa ttcgccaagg aacgtggttg ctccctccaa gggacggaag
     1861 atccagaggt caatcatatc aacgttatta agagaaagac aaactataag aaaagtcaat
     1921 agttttatct aaactatttc ttatttcaat ttgatgattt ggcgatgatt ttagagcacg
     1981 gcaaaaagcc cttgaaaaag tccatttttt caaaggtaat cctgtgttaa ttt
//
LOCUS       DAVKGC010000161         2029 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVKGC010000161 DAVKGC010000000
VERSION     DAVKGC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.161"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            40..1908
                     /gene="pspA"
                     /locus_tag="VW049_001984"
     CDS             40..1908
                     /gene="pspA"
                     /locus_tag="VW049_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEU9433035.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTFVRAEESPVASQSQAEK
                     DYDAAMKNAEDAKKALEDAKAAQKKYADDQKKTEEKAKKEKEASKEEQAANLKYQQEL
                     VKYASEKDSVKKAKILKEVEEAEKEHKKKRAEFEKVRSEVIPSAEELKETRRKAEEAK
                     AKEAELTKKVEEAEKKVTEAKQKVDAEHAEEVAPQAKIAELEHEVQKLEKALKEIGES
                     DSEDYVKEGLRAPLQSELDAKQAKLSKLEELSDKIDELDAEIAKLEKDVEDFKNSDGE
                     YSALYLEAAEKDLAAKKAELEKTEADLKKAVNEPEKPAEEPENPAPAPKPAPAPQPEK
                     PEKPAEQPNPEKPEKPAEQPKPEKPDDQQAEEDYARRSEEEYNRLPQQQPPKAEKPAP
                     APKPEQPVPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGAMATGWLQY
                     NDSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWLQYNDSWYYLNASGAMATGWA
                     KVNGSWYYLNANGAMVTGWLQYNGSWYYLNSNGAMATGWAKVNGSWYYLNANGSMATG
                     WVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV"
BASE COUNT          770 a          369 c          456 g          434 t
ORIGIN      
        1 atagtctaaa aatgactatc agaaaagagg taaatttaga tgaataagaa aaaaatgatt
       61 ttaacaagtc tagccagcgt cgctatctta ggggctggtt ttgttgcgtc tcagcctact
      121 tttgtaagag cagaagaatc tcccgtagct agtcagtctc aagctgagaa agactacgat
      181 gcagcaatga aaaatgctga ggatgcgaaa aaagctttag aggacgcaaa agctgcgcag
      241 aaaaaatatg ccgatgatca aaagaaaact gaggagaaag cgaaaaaaga gaaagaagct
      301 tctaaagagg aacaagctgc aaatctgaaa tatcaacaag agttggttaa atatgctagt
      361 gaaaaagatt cagtaaaaaa agctaaaatt ctgaaagaag tggaggaagc tgagaaagag
      421 cataagaaaa aacgagcaga atttgagaaa gttagatcag aggtaattcc tagcgcggaa
      481 gagttaaaag agactagacg aaaagcagaa gaggctaaag caaaagaagc agaacttact
      541 aaaaaagtag aagaagctga gaaaaaagtt actgaagcca aacaaaaagt ggatgcagaa
      601 catgctgaag aagtcgctcc tcaagctaaa atcgctgagt tggaacatga agttcagaaa
      661 ctagaaaaag ctctcaaaga gattggtgag tctgactcag aagattatgt taaagaaggt
      721 ctccgtgctc ctcttcaatc tgaattggat gctaagcaag ctaaactatc aaaacttgaa
      781 gagttgagtg ataagattga tgagttagac gctgaaattg caaaacttga aaaagatgta
      841 gaagatttca aaaactcaga cggtgaatat tctgcattat atcttgaagc tgcagaaaaa
      901 gatttagctg ctaaaaaagc tgaattagaa aaaactgaag ctgacctcaa gaaagcagtt
      961 aatgagccag aaaaaccagc tgaagagcct gagaatccag ctcctgcacc aaaaccagcg
     1021 ccggctcctc aaccagaaaa accagagaag ccagctgaac aaccaaaccc agaaaaacca
     1081 gagaagccag ctgaacaacc aaaaccagaa aaaccagatg atcaacaagc tgaagaagac
     1141 tatgctcgta gatcagaaga agaatataac cgcttgcccc aacagcaacc gccaaaagca
     1201 gaaaaaccag ctccagcacc aaaaccagag caaccagttc ctgcaccaaa aacaggttgg
     1261 aaacaagaaa acggtatgtg gtacttctac aatactgatg gttcaatggc tacaggatgg
     1321 ctccaaaaca acggttcatg gtactatcta aacgctaatg gtgctatggc gacaggatgg
     1381 ctccaataca acgattcatg gtattaccta aacgctaacg gcgctatggc aacaggttgg
     1441 gctaaagtca acggttcatg gtactaccta aacgctaatg gagctatggc gacaggatgg
     1501 ctccaataca acgattcatg gtattaccta aacgctagcg gcgctatggc aacaggttgg
     1561 gctaaagtca acggttcatg gtactacctc aacgctaacg gtgctatggt aacaggttgg
     1621 ctccaataca acggttcatg gtactaccta aacagcaatg gtgctatggc aacaggttgg
     1681 gctaaagtca acggttcatg gtactaccta aacgctaacg gttcaatggc aacaggttgg
     1741 gtgaaagatg gagatacctg gtactatctt gaagcatcag gtgctatgaa agcaagccaa
     1801 tggttcaaag tatcagataa atggtactat gtcaatggct taggtgccct tgcagtcaac
     1861 acaactgtag atggctataa agtcaatgcc aatggtgaat gggtttaagc cgattaaatt
     1921 aaatcatgtt aagaacattt gacattttaa ttttgaaaca aagataaggt tcgattgaat
     1981 agatttatgt tcgtattctt taggtaccta tcttatgatt tcaggaaat
//
LOCUS       DAVKGC010000162         1981 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVKGC010000162 DAVKGC010000000
VERSION     DAVKGC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.162"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..799
                     /locus_tag="VW049_001985"
     CDS             <1..799
                     /locus_tag="VW049_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU9433036.1"
                     /translation="LSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERSGI
                     YIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMNED
                     GDRLEQEANYFASCFLLPKKEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEYRA
                     FKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSLAT
                     IMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(851..1510)
                     /locus_tag="VW049_001986"
     CDS             complement(851..1510)
                     /locus_tag="VW049_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9433037.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLSIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSITELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCVIIFGKLLL
                     V"
     gene            1580..>1981
                     /locus_tag="VW049_001987"
     CDS             1580..>1981
                     /locus_tag="VW049_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9433038.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGSRIPFLLTLQ
                     ALEEVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQGQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQM"
BASE COUNT          711 a          314 c          434 g          522 t
ORIGIN      
        1 cctaagtaat gtagtgagtg aatcttatca taagggagaa tccattgagg aattggctct
       61 ttatgccagg gaaaaattag gtatttcaaa agataatcat gatttgcttt ataaattaga
      121 acgttcaggc atctatatcg tggaacgatt aattaatggc caagctgatg cttatagcgc
      181 atggtcaaaa ttgggaagac cttatattgt gttaggaacg aataaatcat ctgtacgtcg
      241 aaattttgac ttagctcatg agctaggaca tattctttta cataaatata aagatatgaa
      301 tgaagatggc gatcgtttgg agcaagaagc aaattatttt gcatcatgtt ttttattgcc
      361 aaaaaaagag tttttagtca aatttgaaga gagggttggc aagcgtgtca gcaatcctga
      421 tagttatatt ttattgaagt cggatttgaa tgtttcgata caggctttag agtatcgagc
      481 ttttaagtta ggattattga ctccaaagca acattcttac ttttatcgtc aaattgcgca
      541 aaaaggttac aaaatgattg aacctttgga tgatcaaatt tttgttaaaa aaccaagcaa
      601 agtaaagagt attctggacg tcgttttgag taatcatcta gtcagtctag cgactataat
      661 gtctaaacaa agtattcgtt tacagtttat aagcgaaata ttttcagtcg aaatgaaatt
      721 ttttgatcag tatcaagaag atagaagaac agatcgattt gataacatca tccctttgta
      781 caaaagaaat aatttataat ttaactgtga aataatttca tgaaggacat atggcagaaa
      841 ttggatttta tcagacaagc agtagcttac caaaaataat cacacaagta gcaagtaaga
      901 ggaaaggtac gaaaggaagt ctttcctttt tcttttgcag gagaaaggct aggatacccg
      961 tcgcagaagc aaactgaatc aagatcagta actccgttat gctaaagacg agagcacaag
     1021 aagctaaaaa gaggaaatcc cctgcgccca tgcggatatc gataaaatga gccaaaattc
     1081 caagagcaag gaaggagacc atgaccagat tccagccaga ggaagctatt aggattaggt
     1141 ggaaagtcat ccagaccagt aagggatatt cctgatggcg aaagtcatag atgctcaagg
     1201 tcaaaccagc ggtgattagg acgacttgcc ccaaggaaag caattcccaa gagtaaagca
     1261 gaaagaggag tcctaagcct agttcaaaga gggcatacca gacaggatag cggaccttgc
     1321 agtagcgaca gcgaaagcga ttgaagacct gtgagagaat cggaatcaaa tctaagggac
     1381 gcaagggagt ctgacaggaa tcgcagtgac tggctgaact gataatggat tgctctggaa
     1441 aacggtcaat gaccaaacca agaaaggaag cgagaatgct cccgacgaga aaaaaataaa
     1501 aatcaatcat acttatctat tcgtaaaaaa tgggagaaat agtataatgg agaaacatag
     1561 ataagatggt gaggaaaaga tgacaattcg ttttgaagaa aaggtgagta tagaaaacgc
     1621 tcaatttgta tgccaatggt ccaactccct aggcaaatcc tttcaagaac aatggatggg
     1681 atcaaggatt ccttttctac tgacccttca agccttggaa gaagtctttt caatctttga
     1741 tgaacaagag tttgtgggac ttatccagaa aatcaggcta gaagacagca atcttcatat
     1801 cgggagattt tttatcaacc cccagaaaca ggggcaaggc ttaggtagcc aggctttaag
     1861 gaaatttgtt agtttggcct ttgaaaatga agatatagat agtatttctc taaatgtctt
     1921 cgaggcaaat caaagagctc agaatcttta ccaaaaagaa ggatttgaaa tcgttcaaat
     1981 g
//
LOCUS       DAVKGC010000163         1963 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVKGC010000163 DAVKGC010000000
VERSION     DAVKGC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.163"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            28..621
                     /locus_tag="VW049_001988"
     CDS             28..621
                     /locus_tag="VW049_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9433039.1"
                     /translation="MGMTQQEIADEIGVTKRTYIYWEKGERQIKPEKAQQLADIFGVS
                     VGQLLGYEDYKTIQNDAFDSYRNMAKLLRTNPDFKNIISEYDETNRKNGKLDLSFFVQ
                     AESLPIIEQDIKELILEEWEKTQPEDDYEEIGGTLSDNISRIYIALGQLPILFKDFFG
                     SFLTLSTSDKKIVMQLVNSLYEKNKDIGIIKEYTDKK"
     gene            675..848
                     /locus_tag="VW049_001989"
     CDS             675..848
                     /locus_tag="VW049_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEU9433040.1"
                     /translation="MGFFDTESGNLTELEDVINKQASKGYRLHTITTANGGSKGLGGG
                     DRIQATMVFEKIL"
     gene            895..1434
                     /locus_tag="VW049_001990"
     CDS             895..1434
                     /locus_tag="VW049_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433041.1"
                     /translation="MKKLLTTSAILLTATVLVACSNNQSATKDSSEQPKTGQKNTTST
                     NTKAKVDNSKYDDLISEIKLKLDPESTGAISVKIQNNVIDSDSSEPHDTIMILLTGTA
                     KDSAKETMDAINSNSATTNQQNAITVFRMSISEFAKKLPDDNTTLSLGYEKYADQYDL
                     IAKSSKQKDFIPIGELIVN"
     gene            1605..>1963
                     /locus_tag="VW049_001991"
     CDS             1605..>1963
                     /locus_tag="VW049_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HEU9433042.1"
                     /translation="MRFNFDESGSITTSDKLNNRFFIVAGCQTNDPNKVKRVFRKAKV
                     KYLKHHPELELDIKKEIKGAQMPLDFKDYIFTELMKKTDIQFNFIVFDNHNAMEHLRK
                     KPNITFNYIMFLQVNKL"
BASE COUNT          789 a          323 c          314 g          537 t
ORIGIN      
        1 aggttaaata aagaatttaa cggtaaaatt ggaatgactc agcaagaaat agctgatgaa
       61 ataggagtta caaaaagaac ttatatttat tgggaaaaag gtgaacgtca aataaaacca
      121 gaaaaagccc agcaattagc ggatattttc ggggtaagtg ttgggcagct attggggtat
      181 gaagattata aaactattca aaatgatgca tttgatagct atagaaatat ggcaaaatta
      241 ttacgcacca acccagattt taaaaatata atttcagaat atgatgaaac taatcgaaaa
      301 aacggtaagc tggatttatc tttttttgta caagctgaaa gccttcccat aatcgaacaa
      361 gatattaagg aacttattct tgaagagtgg gaaaaaactc aacccgaaga tgattatgaa
      421 gaaatcggtg ggactctttc tgataatatt tcaagaatct atatagctct tggacaactg
      481 ccaatacttt ttaaagattt tttcggttct tttctaaccc tgtcaacatc tgataaaaaa
      541 atcgttatgc aactagtaaa tagtctatac gaaaaaaata aagatatagg tatcataaaa
      601 gagtatactg ataaaaaata atttagaatt tacttagaat tatctggtca aaaactaaaa
      661 aagagtaact ccccatagga ttttttgaca ctgaatcagg aaaccttaca gaattagaag
      721 atgttatcaa taaacaagct tcaaaaggtt atcgcctgca taccatcaca accgccaatg
      781 gtggaagcaa aggactaggc ggtggagacc gtattcaagc cacaatggta tttgagaaaa
      841 ttctataatc ccccatcttt tatcagaaaa acatcagcat ataggagact acttatgaaa
      901 aaactactaa ccacatcagc aatcttgctt actgctactg ttttagtagc ttgctctaac
      961 aatcaatcag ctaccaaaga tagctcggag caaccaaaaa cggggcaaaa aaacactact
     1021 tcaacaaata caaaagccaa agtagacaac agtaaatatg atgatttaat ctctgaaatt
     1081 aaattaaaat tagatcctga atcaactggc gcaataagcg taaaaattca aaataacgta
     1141 attgattcag attcatccga accgcatgat acaatcatga ttttgctaac tggaacggct
     1201 aaagatagcg caaaagagac tatggacgca atcaattcaa attctgctac aactaatcag
     1261 caaaatgcaa ttaccgtatt tcggatgtct atatctgagt ttgctaaaaa attaccagac
     1321 gacaatacta ctctttccct tgggtatgaa aaatatgctg atcaatacga cttaatcgca
     1381 aaatcttcaa aacagaaaga ttttatccct attggagaac tcatcgtaaa ttaaaaaatc
     1441 ccctatattc gccaatagca accatctttt catggtctat tgtgcgaaaa cagggtaaat
     1501 tgatgaatag aaagccgatt ttacagacta aagcacaaaa aagaaaataa ttattgacaa
     1561 aatagcgttt ttgtcaataa ttaaaaaatt aaaaggagca acaattgaga tttaactttg
     1621 atgaatctgg tagcattacc actagcgata agcttaataa tcgatttttt atagttgctg
     1681 gttgccaaac gaatgacccc aacaaagtaa aaagagtttt tagaaaagca aaggtaaagt
     1741 acttaaaaca ccatcccgaa ctagaattag atattaaaaa agaaattaaa ggggcacaaa
     1801 tgccacttga tttcaaagac tatattttta cagaattgat gaaaaaaaca gatatccaat
     1861 ttaacttcat tgtatttgat aatcacaatg ccatggaaca tctacggaaa aagccaaaca
     1921 taacgtttaa ttatattatg tttctgcaag tcaataaact gat
//
LOCUS       DAVKGC010000164         1958 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVKGC010000164 DAVKGC010000000
VERSION     DAVKGC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.164"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..1131)
                     /gene="ltrA"
                     /locus_tag="VW049_001992"
     CDS             complement(<1..1131)
                     /gene="ltrA"
                     /locus_tag="VW049_001992"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEU9433043.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAVAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVWRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVTSIDERLHTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIAD"
BASE COUNT          501 a          461 c          340 g          656 t
ORIGIN      
        1 atctgctatc catttaggaa cccctaactt aagcaatccc cataatcgtc tcgatttctt
       61 cttccattgc ttccagataa tcactcgtag gcgagtatgc aaacgctcat ctatgctggt
      121 gactatactt ttcatatttc ccaatgagaa atagtttatc catcctcgaa tagacaaatt
      181 cagttgctca atacgtcttg ttaggtctat actccatttc ctctgtgtta gtttcttcaa
      241 tttaagctta aatctccaaa cactatcttg atgtggacga cttttccaac catctgatga
      301 tttccagaac ccgaaaccta gatatttcaa ctctcttggt ctggtaatct tagtcttggt
      361 catgtttact ttcagaccta gccgtttctc aataaaacga ctgactgaat acatcacacg
      421 cttagcgact gcctcgcttc cgaccgtaat cacacaatcg tctgcgtagc gaacaaatcg
      481 aagccctctc ttttctaatt ccttgtccaa ttcattaagc atgatattgg ataagagagg
      541 agataaattt cctccctgtg gcgtgccaac tagcgtttta taacgttgac cgttaatgat
      601 aacacctgaa tgaagatatt tacgaatcaa ggattccgta tctccgtctt cgataatgtt
      661 atgtactaag gacatcaatc tatcttgagg aactgtatcg aaaaatttct ctaggtctat
      721 gtccactatc cactcatagc cgtcatttaa gtactctaag agcttcatga tggctttttc
      781 acatgaccta tttggtctga atccatagct cgtatctgag aaatggggtt cacaaatagg
      841 gctaataact tgaacaatgg cctgttgaat cattctatcc ataactgttg gaattcctag
      901 ttgacggatg cctccgtctg gtttaggtat ctcaacttta agaactggtt gaggcttata
      961 ttttctctgt tttatcagtt ccttagtcaa gcgccagttt tgtctgagat aattatccat
     1021 ctcttcgata gtcattccat caatcccagc tgagccttta ttggatttta cttgattgta
     1081 ggcttccagc atattttcgc gtgataatat cttatctagc agttttgaca tgtgcctatt
     1141 cctttcttgt tttggtgcct gagggacatc ttatattggt gaaccttcat ctagtcatta
     1201 cgtgtcaaag tccagttcta tcagaccact cattttacag acacaagtga agtaggatta
     1261 cttcgattag ttgttcaccc cttcgctcca cttccactac agaagtttcc tcactactat
     1321 gggctcggct gacttctcat gattcgttgt tactacgctt tcggcgctca tgagacctca
     1381 cgggataagc cgtacatctt tcctcgttta tacaagatat gaggacgtta aaaggttaca
     1441 atgaaaatag gaaatctgac gcagagtctt tagactctag gaagatttat ctttttcaca
     1501 tagaccttag tccgaattca attactctcg tgatttacgc atataggtta cgactacctt
     1561 tttggacttt agagttttaa gcccccttat ccgctgtata cgccttacta tcacgttcct
     1621 gttcgtagag cctcgatttc gctatccctt cctctgacga ttcttatttc ctgcgtcaga
     1681 cttaaaacgg tctatcccca gaccgtttta atccgctacc tcacgatagt caggcttggg
     1741 aatcgctatt gggttcaccg gtagcgggtg cccacggagg acttacacct cagatgcacg
     1801 acatgcccgt cgtaccttaa aaagccttgg aaaaaactgt ttccaaggct ttctctatat
     1861 ttgtccttat tgaccaaacc agttacattg ctttatcgta tctgtgattt tagtaatagc
     1921 ctcttcagta ttattctgca accatagata gcgacaaa
//
LOCUS       DAVKGC010000165         1958 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVKGC010000165 DAVKGC010000000
VERSION     DAVKGC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.165"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..1808)
                     /locus_tag="VW049_001993"
     CDS             complement(<1..1808)
                     /locus_tag="VW049_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEU9433044.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNDTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYAASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGKGVDSVYNVPIYYQLKVTNDGSKLTFTYTVTYVNPNTRALENLSRMSYG
                     YSIYNTGTSTQTMLTLGSGLGIPSGVTNSITDKNGRQVRYYNTSTMTKWGSGYTWANG
                     AQMNGWYAKKEYGLTSSWTVPITGTDTSFTFTPYAARTDRIGINYFNGGGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASASASTSASASASTSASESASTSASASASTSA
                     SASASTSASASASTSASASASTSASASASTSASASASTSASVSASTSASASASTSASV
                     SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASASASTSASESA
                     STSASASASTSASASASTSASASASTSASESASTSAS"
     gene            complement(1806..1919)
                     /locus_tag="VW049_001994"
                     /pseudo
     CDS             complement(1806..1919)
                     /locus_tag="VW049_001994"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
BASE COUNT          484 a          421 c          465 g          588 t
ORIGIN      
        1 gaggcgctgg ttgaggccga ttcagaagct gaggtgcttg ctgaggccga ggcgctggtt
       61 gatgctgaag ctgaagcact ggttgacgct gaagccgaag cactggttga cgctgattca
      121 gatgctgatg tgcttgctga agccgaggca cttgtcgatg ctgaggctga agcacttgtt
      181 gatgctgatt cagatgctga ggtacttgct gaagctgagg cactggttga tgcggattca
      241 gacgcgctgg tgcttgctga ggccgacgca ctggtgcttg ctgagactga tgcactcgtt
      301 gatgctgaag ccgaggcgct ggtgcttgct gagactgatg cactcgttga tgctgaagcc
      361 gaggcgctgg tgcttgctga agccgacgca ctggtacttg ctgaagctga cgcactggtt
      421 gatgctgaag ccgacgcact cgttgatgct gaagccgacg cactcgttga tgctgaggct
      481 gaggcagacg ttgatgcgga ttcagacgca cttgttgatg ctgaagctga ggcactcgtt
      541 gatgctgaag ccgacgcact tgttgatgct gaagctgagg cgctttgact agcacttact
      601 gagagtgact tagactgtga aagtgactga ctggtcgtgc tagattcaac tacctttcct
      661 ccaccattga agtagttaat tccaattcta tctgttctag cagcgtaagg ggtaaatgta
      721 aaggatgtat ccgttccagt aattggtaca gtccaagatg atgttaatcc atattctttc
      781 ttagcatacc aaccattcat ttgggcacca tttgcccaag tatacccact accccacttc
      841 gtcattgtag atgtattata gtatcgaacc tgtctaccat ttttgtcagt aatggagttc
      901 gttacacctg aagggatacc aagaccactg ccaagggtta acattgtttg tgttgaagta
      961 cctgtattat agatagaata cccataagac attcttgaaa gattttcaag agcccttgtg
     1021 ttaggattta cataggtgac tgtataggta aaggtcaatt ttgaaccatc atttgtcact
     1081 ttcaattgat agtaaattgg aacattatat accgaatcta cacctttacc tgtataaact
     1141 tttgtctcac ttttagcaat gtttagactt gcattaattg caggggcgcc atttacaatc
     1201 gtattgccag aaaaaacagc agcttttata gaagccagca attgctcgat ggaatccact
     1261 gaacgcttgc gtcgcttagc ataagattgg agattgacat ttgtaactga tgctgcataa
     1321 tcactagctg gtttcttacg atcttcttcg accttcttag cagttgcttc tgtagcggca
     1381 gctgtttgtg atcctaccac agtagatgat gcagagatac ttgtcgaagc cgatgtactt
     1441 gctgattctg atgcacttgt actcgctgag gtcgaagcgc tggttgaggc tgactcagat
     1501 gcagatgtac ttgccgactc tgaagctgac aaactagttg aactcgctga attacttgta
     1561 gatatcgtac ctagaactac tgtatcattt gttgccaaag catccgtttt ctctacagtt
     1621 ttttcaagta ctgccgactc atttgtaaat accttcgttt gagttgcaac ggttccactt
     1681 atgacagttc ccgcagcaac tattcccttg aggatatcaa gcccagtaat tgaatgactt
     1741 actttatctt ctaccgtttc ggtcatgacc tgagtagtat caacaccacc acgcatgacc
     1801 ttaaacaagc caaaaagaga agtcgaggcc cgtagccaat gcttccctga tttgattaat
     1861 ttgaaccgag ttactcggtc tgtttctcta tattcaccct tctgacgtct aaaaaatatt
     1921 ttgaaatttc ctttcgagta ttatttttat gtatggat
//
LOCUS       DAVKGC010000166         1944 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVKGC010000166 DAVKGC010000000
VERSION     DAVKGC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.166"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..116)
                     /locus_tag="VW049_001995"
     CDS             complement(<1..116)
                     /locus_tag="VW049_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU9433045.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEA"
     gene            complement(187..399)
                     /locus_tag="VW049_001996"
     CDS             complement(187..399)
                     /locus_tag="VW049_001996"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9433046.1"
                     /translation="MEDFFAWCRRQSVLAGSKPGRAIEYSLKYEETFKTILKDGHLVL
                     SNNLAERAIKSLVMGRSKRVHWTLLA"
     gene            complement(329..625)
                     /locus_tag="VW049_001997"
     CDS             complement(329..625)
                     /locus_tag="VW049_001997"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9433047.1"
                     /translation="MVNCEPLEAYRQLEEAELVGCWAHVRRKFFEATPKQADKSSLGA
                     KGLAYRDQLFALERDWEALPADERLQKRPALNGRLLCLVPPSVSFSRFKTRKGN"
     gene            complement(698..1138)
                     /locus_tag="VW049_001998"
     CDS             complement(698..1138)
                     /locus_tag="VW049_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9433048.1"
                     /translation="MKAPIPKAPLAHSLGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLREKLLEQALLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHDQRRSGSVVQEFLGNYSGYMHCDMLRQ"
     gene            1129..1436
                     /locus_tag="VW049_001999"
                     /pseudo
     CDS             1129..1436
                     /locus_tag="VW049_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1591..1914)
                     /gene="tnpB"
                     /locus_tag="VW049_002000"
     CDS             complement(1591..1914)
                     /gene="tnpB"
                     /locus_tag="VW049_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9433049.1"
                     /translation="MTIHLSSLGQVYLVCGKTGMRQGIDSLAYLVKTHFELNSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPKI"
BASE COUNT          587 a          412 c          374 g          571 t
ORIGIN      
        1 gcctctaaaa cctctttgtt tacgagagtt tcctcgtttg gaagacattc tagaagatag
       61 gatagatatt tctcactatt tagttgatga cgtttagctg tttccaacaa actcataata
      121 atagctcttg ctttagctcc ttcaaaactt tgagaaaaca accaccctcg cttttttaaa
      181 ctgagcttag gctaaaagag tccagtggac tcttttactc cgtcccataa ccaatgattt
      241 aatggcgcgt tcagctagat tattggaaag gaccagatgt ccgtctttta aaatggtctt
      301 aaaggtttct tcatacttga ggctgtattc aattgccctt cctggttttg aacctgctaa
      361 aactgactga cggcggcacc aagcaaagaa gtcttccatt aagggctgga cgtttctgta
      421 gtcgttcatc agctggcaaa gcctcccagt ctctttccaa ggcaaataac tggtcacgat
      481 aggctaaacc tttagctcct aaggatgatt tatctgcttg cttgggggtc gcttcaaaaa
      541 atttccttct cacatgcgcc caacatccaa caagttcagc ttcttccagt tgacgataag
      601 cttctagcgg ttcgcagttt accaagctta gcgtcgcctt gctcctaaac cttagactgc
      661 tatcgcatag gcagaactag aggactaaag ttctaagtta ctgccgcaac atatcacaat
      721 gcatatagcc agaataattt cctaggaatt cttgcactac cgaaccactc cgacgctgat
      781 catggtggta cagcgtaatc ccttgtttct cagctttccc agacaaaaaa gtccaatagt
      841 aagtcagctg actatcactt tctaagaccc gataagaggt ttcatctgca tgaagaagag
      901 cttgttctaa caacttttct cgtaaaaggt tataaagggg ctccaaatag tattgactcg
      961 tcttgatatg ccaattagag atttccttac gtgtgattgg taaacccatc ctagcccaat
     1021 cttcttcttg gcgataattg ggtaccttca gattaaactt ctgatggatg gtgtgagcga
     1081 taatagaagc tgagccaagg ctatgcgcca aaggggcttt aggaatagga gctttcacaa
     1141 gcttatccag atgattatct tttactcgtt atggacaatg ctatatggca taaatcaagt
     1201 accttaaaga ttccgactaa tattggcttt gcatttattc ctccatacac accagagatg
     1261 aacccattga acaagtgtgg aaagagattc gtaaacgtgg atttaagaat aaagcctttc
     1321 aaactttgga agatgtcatg aatcaacttc aagatgttat acaaggactg gagaaggagg
     1381 tgataaagtc catcgttaat cggagatgga ctagaatgct ttttgaaagc agatgagtat
     1441 aaaaagaaag tcctcatttc aatagaaatc acgactttct gatggattta tagtaaaatg
     1501 aaataataac aggacaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
     1561 gcaaaggtgt actattctag tttcaatcta ctatattttt ggagtgatag aaaagccttt
     1621 catcagccaa tctacttgtt caggtgcgag agctttgaca tccttttctg tactgggcca
     1681 agtcagtctg ccgttctcaa agcgtttata taatagccaa aatccttgac catcccagta
     1741 aaggacttta aagcggtctt tacgtccacc acaaaagaga aaaacttgac ctgagaaaga
     1801 attcaattca aagtgggttt taaccagata agccagtgaa tcgattcctt gtctcatacc
     1861 cgttttccca catacgagat agacctgccc taggctagat agatgaattg tcatagagca
     1921 acaccttatc caaaagctgt tcta
//
LOCUS       DAVKGC010000167         1924 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVKGC010000167 DAVKGC010000000
VERSION     DAVKGC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.167"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(47..283)
                     /locus_tag="VW049_002001"
     CDS             complement(47..283)
                     /locus_tag="VW049_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9433050.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            543..1358
                     /locus_tag="VW049_002002"
                     /pseudo
     CDS             543..1358
                     /locus_tag="VW049_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259226.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1393..1924)
                     /locus_tag="VW049_002003"
                     /pseudo
     CDS             complement(1393..1924)
                     /locus_tag="VW049_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          673 a          378 c          280 g          593 t
ORIGIN      
        1 ctttccataa aagagtaaat tagttcggaa atttactaaa attacctcaa caagaaataa
       61 aaccccgatt cattaccaat ttttcaagat acaaacgata agcaacacga taatggtaaa
      121 cgataaaatc cctacgacaa ccaatgccat atctcactaa ataataaatt aaaaatttaa
      181 aatgaacatg ttcccagtca aaattatcac caaatgtagg accatactct tcttcaatac
      241 tatcatagaa attagccatc tgctcataaa ttttttgtaa cataatcaaa tactcctttt
      301 cttttttata aacttattct aacaaaaaat tttacacatt cactatcaat tcctgaattg
      361 ttaaaatacc agcctcaaca agataaaaat aggaaaagtt gacagaatag aaacaaattt
      421 gctacctaaa tttcaaacga ttaaagttct aaaaagaccg ccttaaaact tttgggtaaa
      481 tcctattaga actataatat ttttatatag gtattatggt tctgattttc tagtataaaa
      541 aaattcaaac tagaattctt cttataaaag taaaaggccg ttttaagtgc tatcattgct
      601 caaaaatgga ggtcgctgag acttctatcg tcaagaagaa tcatcaaatt ccttgtatca
      661 tcaaccaaaa gattgctcag aagctaattg aaaagacttc tatgaccgac attgatcatc
      721 agttgtctat ttcaatttca acttcaactg tcattcgcaa gatcaatgat tttcactttg
      781 agcatgattt ttcgcgtctt cctgagatta tgtcctagga cgttgaaaca gtccggggag
      841 tgactgtttc aatcgggaga tggagatgag ctttattgct caagactttg ataagctcaa
      901 tatcataact gttcttgaga gcagaacaca agccatcatc cgaaatccca tgaatacaag
      961 gctatcaagc gatactgaaa gctcattcaa caagatagtc agaaactgag tgataaacga
     1021 ttttatcgcc ctacttttcg tatgcacttg actaataaag aaattctaga taaacttttg
     1081 agctattccg aagacttgaa acaccactat aatctctatc agctcttgct tttccacctc
     1141 tttttcagac tctgtttaaa accttcctca aagataaaga gaaaatcgtc aacgcccttc
     1201 aattacctta ttccaacgcc aaactggaag ccaccaataa tctcatcaaa cttatcaagc
     1261 acaatgcctt tggttttagg aactttgaaa acttcaaaaa agaaaggacg aaatttgtcc
     1321 tttctagatt ttcgctttct tcaacccact acagttgaca aagaaccgaa aaatgtctcg
     1381 cctctaatat ggtcaattga aacaagaaca agacaaaaga gcctcataaa aggtattgca
     1441 acttggtaat acctttttgg ggttcttttt gatatgaacc catgttttct caataggatt
     1501 gtactcaggt gagtagggag gaagaggtaa aagtttatgc ccaaactctt cacacaagag
     1561 ttctaactta cccattctat ggaatcttgc attgtccata ataataaccg atggtgtggt
     1621 taatgttggt aagagaaact tctgaaacca agcttcaaaa aagtcgctcg tcaccatctc
     1681 ttcgtaagtc attggagcga ttaactcacc atttgttaga cctgcaacca aagaaatcct
     1741 ctgatatctt cttccagata ctttacctct tattaactga ccttttaatg agcgaccata
     1801 ttctcgataa aaataagtat cgaatcctgt ttcatcaatc taaacaggtg ctaggtgctt
     1861 taaactatta aaatttttaa gaaataaggc tactttttct gggtcttgtt catagtaggt
     1921 gtgg
//
LOCUS       DAVKGC010000168         1799 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVKGC010000168 DAVKGC010000000
VERSION     DAVKGC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.168"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..>1799
                     /locus_tag="VW049_002004"
     CDS             <1..>1799
                     /locus_tag="VW049_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9433051.1"
                     /translation="TKEILSVTPMNNNNFVTDLGDANKIIVHYADGTKDYFNLSSSDE
                     GLSNVREYTITDLGIKYTPNIVQKDHSALINGIVNILSPIELQSNPIYQKLGRTGSNR
                     VNAIKDLYLEESFDHVKANLNILVTKLVENEDHQLNNSSAAQQMILDKVEKNKAALLL
                     GLTYLNRYYGVKFGDVNIKELMLFKPDFYGKNVSVLDRLIEIGSREDNIKGHRTYDAF
                     SEIISKTTLSENLVNFLNYNRRLFTADVNIYDWFKKATEGNVYIAERASEVDEIKAAK
                     YKVWDNLQSATHRKMILPLLNLNKSHVYLISNYSAMAVGTAEKLGHSSFDGIKNQIEK
                     AADSYRNYFDFWYRLSLDKVKNMLVKNTVTPLWEGYQAPGGWPDKYGKRNNKEDYAPL
                     RELFGPIGKYYGNNGTGAYAVIWDNPLDTRTEVNYIALSMIDEFGISVYTHETTHVND
                     RAIYLGGYGRRSGTHAEAYAQGMLQTPVPSTWFDEYGALGINMTFYRPNDGNQWYITD
                     PKTLKTREDIDNYMKGYIDTLSLLDYVEGDAVISKHDDKLNQLWFKKVDPIERTVGSK
                     DQYDKVRNMSSDDWKGITLNSVNDLVDNNLMTN"
BASE COUNT          651 a          246 c          344 g          558 t
ORIGIN      
        1 tactaaagag attctatcgg ttactcctat gaacaataat aactttgtaa ctgatttagg
       61 tgatgcaaac aaaattatcg ttcattatgc tgatggaaca aaagactact tcaacctatc
      121 ttctagtgat gaaggtctaa gtaatgttag agaatacact ataactgact taggtattaa
      181 atatacacct aatatcgttc aaaaagatca tagtgcttta attaacggta tcgtaaatat
      241 cttaagccct attgagcttc aatctaatcc aatttaccaa aaacttggca gaacagggtc
      301 taatagagta aatgcaatta aagaccttta tttagaagaa agttttgacc atgtaaaagc
      361 taatttaaac atattagtaa ctaaattagt tgaaaatgaa gatcatcaac tgaataattc
      421 atctgcagct caacaaatga ttcttgataa agttgagaaa aataaagctg ccctattatt
      481 aggtttaaca tacttaaacc gttactacgg tgttaaattc ggtgatgtta acattaaaga
      541 attaatgtta ttcaaaccag atttctatgg taaaaatgta agtgtattag atagattaat
      601 tgaaatcggt tcaagagaag ataatataaa aggtcatcgg acatatgatg ctttcagcga
      661 gattatttct aagacaacct tatcagaaaa tttggtaaac tttttaaatt ataatcgtcg
      721 actatttact gcagatgtaa atatttatga ttggtttaag aaagctacag aagggaatgt
      781 ctatatagcg gagcgtgctt cggaagtcga tgaaattaaa gcggcgaaat ataaagtttg
      841 ggataattta caatctgcta ctcatcggaa gatgatactc ccattattaa atctaaataa
      901 atcacatgtg tatttaatct cgaattatag tgcaatggct gttggaacag cagaaaagtt
      961 agggcatagc tccttcgatg gaataaaaaa tcagatcgaa aaagcagcgg acagttatcg
     1021 aaattacttt gatttttggt atagattatc tttagataag gttaagaata tgctagtcaa
     1081 aaatacagta actcctcttt gggaaggtta tcaagcacct ggtggatggc ctgataagta
     1141 tggtaagagg aataataaag aagactatgc cccattgaga gagttgtttg gacctattgg
     1201 taagtattat ggtaacaatg gaacaggtgc ttatgcagtt atttgggata atcctttgga
     1261 tacgcgaaca gaagtaaatt atattgcatt aagtatgatt gatgagtttg gaatctctgt
     1321 ttatacacat gaaactactc atgttaatga tcgtgcaatc tatttaggag gatatggacg
     1381 tcgctcaggg actcatgcag aagcctatgc acaaggaatg ttacaaacac cagtaccaag
     1441 tacatggttt gatgaatacg gtgctttggg aattaatatg actttctatc gtccaaatga
     1501 tggaaatcag tggtatatta ccgatccgaa gactcttaaa acaagagaag acattgataa
     1561 ttatatgaaa ggttatatag ataccttgtc tcttcttgat tatgttgaag gtgatgcagt
     1621 cattagtaaa catgatgata aattaaatca actgtggttt aagaaagttg atcctattga
     1681 acgcactgta gggtcaaaag atcaatatga caaggttcga aatatgagtt ctgatgattg
     1741 gaaaggaata acattaaaca gtgttaatga cttagttgat aataatctga tgactaatc
//
LOCUS       DAVKGC010000169         1794 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVKGC010000169 DAVKGC010000000
VERSION     DAVKGC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.169"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..618)
                     /locus_tag="VW049_002005"
     CDS             complement(<1..618)
                     /locus_tag="VW049_002005"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9433052.1"
                     /translation="MRKEIKAVYAILNYNTWEDTARLAQKVATFQHIQSVIIVDNLST
                     DDSYHYLKRLEGEKISVYQTQRNGGYSVGNNFAARKAYNMGVDILFISNPDVDIDEKD
                     SLMIAQNLYKNSSYALLSGIEYNAMKEIDLPIVWHENSYYDDLLDCLFLTRKWRRKKE
                     DINLSMIKSSIIDVDLVKGSFFAVRLSDFHDVGYFDESVFLFCEER"
     gene            complement(630..1316)
                     /locus_tag="VW049_002006"
     CDS             complement(630..1316)
                     /locus_tag="VW049_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU9433053.1"
                     /translation="MIVKSFIKKITGKTVDIHPDVPLSYILQRGINYSFGLFRGVVRG
                     IGFGQNDKRLFIGQGVSILAKRKLFVGKNVRIGKKVSIDALSKEGIHFADNVKIGDYS
                     QIIGTGSIKNMGIGLKIGKNSSFSEYSLFGSAGGITIGDNVIAGQNVRFHAENHNYSD
                     LDKLIVEQGVSRKGISVGDNCWIGAGSVFLDGSSIGSGCVVAANSVITKNFPDNVIVA
                     GVPAKIIKMK"
     gene            complement(1306..>1794)
                     /locus_tag="VW049_002007"
     CDS             complement(1306..>1794)
                     /locus_tag="VW049_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9433054.1"
                     /translation="HHQRLTTTNNKIRVAYIGPDEEYKGYFDFVDFVETLDRESYEVA
                     TYGHLPNEECPSFIEQKGYFTKEMIDSVYENIDILIVPSKWKETFGLITVEALSYGVN
                     VFVSENVGSKDLLPESHVFKNQNDLIVKFLKNDIENTKLKTLDEHSIEVIQYYERVIN
                     DS"
BASE COUNT          600 a          363 c          205 g          626 t
ORIGIN      
        1 tctctcttca caaaaaagaa atacagattc atcaaaataa ccaacatcat gaaaatcaga
       61 cagtcttaca gcaaaaaacg atcctttaac aagatctaca tctattatag atgatttaat
      121 cattgacaaa ttgatatctt ctttttttct tcgccatttt ctggttaaaa acaaacaatc
      181 aagtaagtca tcgtaatagg aattttcatg ccagacaatt ggcaaatcta tctccttcat
      241 agcattatat tctatacctg atagcagtgc atatgaacta tttttataca aattttgtgc
      301 aatcatcaag gaatcttttt catcaatatc aacgtctgga tttgaaatga aaagaatatc
      361 aactcccata ttataagctt ttcgtgctgc aaaattattt ccaacagaat atccaccatt
      421 tctttgtgtt tgataaactg aaattttctc tccctcaagt ctttttaaat aatggtatga
      481 atcatctgtt gatagattat ccactattat cacagattga atatgctgaa atgttgcaac
      541 tttctgtgct aaacgcgcag tatcttccca agtattgtaa tttaatatcg cataaacagc
      601 tttaatttct ttcctcattt aacatcctat catttcattt ttataatttt agctggtact
      661 ccagcaacaa taacgttatc tggaaagttt ttcgtaatta cagaatttgc agctacaaca
      721 caacctgaac caatgctgga cccatccaaa aaaacagatc cagcaccaat ccaacaatta
      781 tcacctactg aaattccctt tcgagatacc ccttgttcaa caatgagttt atctaaatca
      841 ctataattat gattttcagc atgaaaacga acattctgac ctgcgataac attatcacca
      901 attgtgatac ctccagcaga accaaataaa ctatattctg aaaaagatga gttcttaccg
      961 attttcaagc caatccccat attttttata gaacctgtgc caataatttg agaataatct
     1021 ccaatcttta cattatctgc aaaatggatt ccttcttttg acagtgcatc tatagacacc
     1081 tttttaccaa ttcgaacatt ttttccaaca aataatttac gctttgctaa gatagaaact
     1141 ccttgtccaa tgaataaacg tttgtcgttt tgaccaaatc cgattccacg tacaacccca
     1201 cgaaagagtc cgaaactata gtttatacct ctttgtagta tatacgacaa aggtacatct
     1261 ggatgaatgt ctactgtttt tccagtaatc ttttttatga aactcttaac tatcatttat
     1321 cactctttca taatactgaa tcacttctat cgagtgttca tctaaagttt tcaattttgt
     1381 attttctata tcatttttta aaaatttaac tattaaatca ttctgatttt taaaaacatg
     1441 cgattctgga agtaagtctt ttgacccaac attttcactc acgaaaacat ttaccccata
     1501 ggataatgct tctactgtta tcaatccaaa tgtttccttc cacttacttg gaacaataag
     1561 aatatcaata ttttcataga cactgtcaat catttcctta gtaaaatatc ctttttgttc
     1621 aatgaatgaa ggacactctt catttggtaa atggccataa gttgccactt cataggactc
     1681 tcgatctaaa gtttcaacaa agtcaacaaa atcaaaatat cctttatatt cttcatctgg
     1741 accaatataa gcaactctaa ttttattatt tgtagttgtc aatctctgat gatg
//
LOCUS       DAVKGC010000170         1785 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVKGC010000170 DAVKGC010000000
VERSION     DAVKGC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.170"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..959)
                     /locus_tag="VW049_002008"
     CDS             complement(<1..959)
                     /locus_tag="VW049_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9433055.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGS"
     gene            complement(959..1774)
                     /locus_tag="VW049_002009"
     CDS             complement(959..1774)
                     /locus_tag="VW049_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9433056.1"
                     /translation="MFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGASLSVVIG
                     LSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSMALSSFV
                     SFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLNALAMGD
                     EMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISKDIRTMT
                     ISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVNN"
BASE COUNT          656 a          322 c          266 g          541 t
ORIGIN      
        1 gaacctaaca agccagttac aacacctact ggatatctag ctggtaaaat attttgagag
       61 aatatgtctg ataacaaaac tagtaaaatt ccaaccaatc cagctaatat tgggcttctt
      121 ttcttgccaa tatttaaggc tatgggacca gctaaaaaag atatacaagc tattggtcct
      181 gtaattgaag tagaaaaagc agttaaagat acagcgcaaa aaattaaaac aagccttgaa
      241 agctcgggat ttgctccaag tccgattgct atttcttcac caagttcaat aatttctagt
      301 cttttattaa aaaataaaac taatatagta gcaataatac ttactattag aacaaggggt
      361 atgtcatcta actttgtaaa agataaagag ccactgagcc atctcataac ttcttgtaat
      421 tcatatcttg ctactttcaa caataaaaat gaggtgcctg ctcttgtgac agcttgaaaa
      481 ccaataccta atattatcag tcttgctgct gaaaaaccat cttttttagc tagtaaaaat
      541 aatattaaag atgatgttag tccacaagtt attgaaataa ttccagtagt taaactattt
      601 gtttttaata ccaatatgca aaagaccgct gcaatagatg aagaacttgt gacaccgatt
      661 atatcaggac ttgcaagagg atttcttaac atagtttgaa agataaatcc tgccaatcca
      721 aaagaccagc cagctataat tcctgctaat aattttggta atctaatttc cataatcgaa
      781 aaactagctc caggaacagt ttcactattt aagactttaa tcaaagttga aaaagaataa
      841 ctttcatctc cgataagtaa aatgaaaaat gatagactga ttattattaa taaaaatagt
      901 gaggaaaata gtgttattct attttttctt ttttgaatac ctataattaa attttgcatt
      961 agttattaac ccctctattt ttcatagtta cataaataag tactggaccc ccgattattg
     1021 cagtaattat ccctacttca atttcacctg gtttacctaa catacggccg attatatcac
     1081 atataagcaa gagctctgca cctataaaag atgaagaaat ggtcattgtg cgtatatctt
     1141 tgcttataaa taagccacaa aagtgaggaa ctataagacc tacgaagcca ataggtccac
     1201 caattgcagt aatacttgaa cataaaagca cacttgcaat tattgcaagt gatcttatcc
     1261 tattaacatt aactccaaga ccaacagcca tttcatcacc catagctaaa gcgtttaaat
     1321 ctgatgaaat aaatatagct atcaagtgac ctaaaattat aaaaggtagt agtgtagata
     1381 tagaagataa tgtagctgct ccaaggctac ctatttgcca aaatctaaat ttgtctaaga
     1441 cgttattatt cggtaaaatt aaaaaactta caaaactgct taaagccata ctaacacaag
     1501 ttcctgataa ggcaagtttt ataggggtaa ggcctgcttt tccgcttaca gcaatcgcgt
     1561 atacaaaaat tgcacttact aagccaccaa tgattgcaaa acttatatgg cttatgcttg
     1621 atgaaattcc taaaaaagaa agaccaatta ctacactaag acttgctcct gtgtttatac
     1681 cgagtatacc tggatcagct attgggtttc tagtaactga ttgcatcaat acaccgctta
     1741 tggcaagact agaacctgct aaaattgcaa aaatagttct aggta
//
LOCUS       DAVKGC010000171         1746 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVKGC010000171 DAVKGC010000000
VERSION     DAVKGC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.171"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            329..805
                     /locus_tag="VW049_002010"
     CDS             329..805
                     /locus_tag="VW049_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9433057.1"
                     /translation="MIGKNIKSLRKTHDLTQDEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            805..1581
                     /locus_tag="VW049_002011"
     CDS             805..1581
                     /locus_tag="VW049_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU9433058.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKFSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSSVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLRVVEERHKELEGKNSYGI"
     gene            1571..>1746
                     /locus_tag="VW049_002012"
     CDS             1571..>1746
                     /locus_tag="VW049_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9433059.1"
                     /translation="MEFKKLVIENFRNFSSIEVALSNKNVIFGMNDSGKTNLLFAIRY
                     LLDRTIRNKGFIKS"
BASE COUNT          644 a          225 c          341 g          536 t
ORIGIN      
        1 tttgctaata gcctaataat cacatgtgga gtgttgctac aacgaaaaag gtgataatcc
       61 ttgatttcaa gctattttat aagcattttg tctttgtaga taaaggcaat tttgacaata
      121 aaaatcctaa aaggtgaatc gttatagatg tatttgtaga tatcgtttgc gcatcgaaaa
      181 aattaataca agaataaata tttatggctc tttaggtgac ttttatagaa gtaaagttta
      241 ggatagaaaa acaagaaata atgcaccatt tttggtgcat tatgcttttt tatgctataa
      301 ttgaattata aaaataaagg agtttgccat gattggaaag aacataaaat ccttacgtaa
      361 aacacatgac ttaacacaag acgaatttgc acggattgta ggtatttcac gaaatagtct
      421 gagtcgttat gaaaatggaa cgagttcagt ctctaccgaa ttaatagaca tcatttgtca
      481 gaagtttaat gtatcttatg tcgatatcgt aggagaagat aaaatgctca atcctgttga
      541 agattatgaa ttgactttaa aaattgaaat tgtgaaagaa agaggtgcta atctattatc
      601 tcgactctat cgctatcaag atagtcaggg aattagcatt gatgatgaat ctaatccttg
      661 gattttaatg agtgatgatc tttctgattt gattcatacg aatatctatt tagtagaaac
      721 ttttgatgaa atagagagat atagtggcta tttggatgga attgaacgta tgttagagat
      781 atctgaaaag cggatggtag cttaatggaa atccaagatt atactgatag tgaattcaaa
      841 catgctttag caaggaatct tcgttcactg acaagaggaa aaaagtccag taagcaacct
      901 atagcgattt tgcttggagg tcaaagtggt gccggtaaga ctacaattca tcgtattaaa
      961 cagaaagaat ttcaaggaaa tattgttatc atagatggtg atagttttcg ttctcagcat
     1021 ccacactatt tagaactgca gcaagaatat ggcaaagaca gtgtagaata taccaaagat
     1081 tttgcaggaa aaatggtaga gtctttagta acaaaattta gtagtttgag atacaatctt
     1141 ttgatagagg gaactttacg aacagttgat gttccaaaga aaacagcaca actcttgaaa
     1201 aataagggat atgaagtaca attggcctta attgcgacaa agcctgaatt gtcgtatcta
     1261 agtactctta tccgttatga agaactgtac attatcaatc caaatcaagc acgcgcaact
     1321 ccaaaagaac atcatgattt cattgtaaat catctagttg ataatacacg acaattggaa
     1381 gaactggcta tctttgaaag aattcaaatt taccaacgag atagaagttc tgtatatgat
     1441 tcaaaagaaa atacaacttc agcagcagat gttcttcaag agttactctt tggggagtgg
     1501 agtcaggtag agaaagagat gttgagggtg gtggaagaaa gacataaaga gttagaagga
     1561 aaaaatagtt atggaattta aaaaactagt aattgaaaat tttcgtaatt ttagttctat
     1621 tgaggttgct ttatcaaata aaaatgttat atttggaatg aatgattcag ggaaaacaaa
     1681 ccttctattt gcaataagat acctcttgga cagaactata cgaaataaag gttttattaa
     1741 atcgga
//
LOCUS       DAVKGC010000172         1733 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVKGC010000172 DAVKGC010000000
VERSION     DAVKGC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.172"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            16..228
                     /locus_tag="VW049_002013"
     CDS             16..228
                     /locus_tag="VW049_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9433060.1"
                     /translation="MTQPLLHADETSYRVLESDSQLTYYWTFLSGKAEKQGITLYHHD
                     QCRSGSVVQEFLGDYSGYVHCDILRQ"
     gene            323..752
                     /locus_tag="VW049_002014"
                     /pseudo
     CDS             323..752
                     /locus_tag="VW049_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            823..981
                     /locus_tag="VW049_002015"
     CDS             823..981
                     /locus_tag="VW049_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU9433061.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            1074..1733
                     /locus_tag="VW049_002016"
                     /pseudo
     CDS             1074..1733
                     /locus_tag="VW049_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          551 a          316 c          383 g          483 t
ORIGIN      
        1 ttgcgagaga gactattgac tcagccctta cttcatgcgg atgaaacttc ttatagggtg
       61 ctagagagtg atagtcagct gacttactat tggacttttt tgtcaggtaa agcagagaaa
      121 caagggatta cgctttacca ccatgatcag tgtcgaagtg gttcagtagt acaagaattc
      181 ctaggagatt attctggcta tgtgcattgt gatattttgc ggcagtaact taggacttta
      241 gtcctctagt tctgcctatg cgatagcagt ccaaggttta ggagcaaggc gacgctaagc
      301 ttggtaaact tcgaaccgct cgtctgctta tcgtcaactg gaagaagctg aacttgttgg
      361 atgttgggcg catgtgagaa ggaagttttt tgaagcgccc ccccaagcaa gcggataaat
      421 catccttagg agctaaaggt ttagcttatt gtgatcagtt attttccttg gaaagagact
      481 gggaggcttt gccagctgat gagcgactac agaaacgtca agaacatctc cagcccttaa
      541 tggaagactt ctttgcttag tgccggcgtc agtcagtttt agcaggttca aaactaggaa
      601 gggcaattga atacagcctc aagtatgaag aaacctttaa gaccattttg aaagacggac
      661 atctggtcct ttccaataat ctagctgaac gcgccattaa atcattggtt atgggacgga
      721 gtaaaagagt ccagtggact cttttagcct aagctcagtt taaaaaagcg agggtggtta
      781 ttttctcaaa gttttgaagg agctaaagca agagctatta ttatgagttt gttggaaaca
      841 gctaaacgtc atcaattaaa tagcgagaaa tatctatcct atcttctaga atgtcttcca
      901 aacgaggaaa ctctcgtaaa caaagaggtt ttagaggctt atttaccatg gactaaagtt
      961 gtacaagaaa agtgcaaata agaaatctcc agattaggaa ctatccgtga gttctccagt
     1021 ctggagattt ttcaatagac ttcctgcgaa acaaaatatg gtataatagt tctatgaatg
     1081 atgaagcaag taaacaacta accgatgcac gatttaagcg tcttgttggt gttcaacgca
     1141 cgacttttga agagatgtta gctgtattaa aaacagctta tcaacttaaa cacgcaaaag
     1201 gtggacgaaa acctaaatta agtctagaag accttcttat ggccactctt caatatgtgc
     1261 gagaatatcg aacttatgaa caaattgcgg ctgtttttgg tattcacgaa agcaacttaa
     1321 tccgtcggag ccaatgggtt gaagtaactc ttgttcaaag tggtgttacg atttcaagaa
     1381 ctcctctcag ttctgaggac acggtaatga ttgatgcgac ggaagtaaaa atcaatcgcc
     1441 ctaaaaaaag aattagcgaa ttattctggt aaaaagaaat ttcacgctat gaaggctcaa
     1501 gcgattgtca caagtcaagg gagaattgtt tctttggata tcactgtgaa ctattgtcat
     1561 gatatgaagt tgttcaaaat gagtcgcaga aatatcagac aagctggtaa aatcttggct
     1621 gacagtggtt atcaagggct catgaagata tatcctcaag cacaaacttc acgtaaatcc
     1681 agcaaactca aaccgctaac aattgaagat aaagtctata accatgcgct atc
//
LOCUS       DAVKGC010000173         1700 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVKGC010000173 DAVKGC010000000
VERSION     DAVKGC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.173"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..603
                     /locus_tag="VW049_002017"
     CDS             <1..603
                     /locus_tag="VW049_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9433062.1"
                     /translation="EHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIE
                     SGSTCALLAEEICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPL
                     LKEMIQTFHVHQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDK
                     RGTVRRFALSQVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            616..1596
                     /locus_tag="VW049_002018"
     CDS             616..1596
                     /locus_tag="VW049_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9433063.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT          614 a          187 c          343 g          556 t
ORIGIN      
        1 gagcatggat atgctgtatt aaatagtgga gatgacttaa atgtacgttt gtcaattaat
       61 tatgaaatta agagaaaaat tgttcaggaa gcagtaaaat tggtgtcaga taatgaaaca
      121 ataatgatag aatctggatc gacctgtgct ttacttgctg aggaaatttg caagcaaaaa
      181 agaaatgtta cgattgtaac aaattcattt tttatagcaa attttgtgag agcttatgat
      241 tcatgtcgta ttattgttct tggtggagag tttcagaagg attcacaggt tactgtggga
      301 cctttattaa aagaaatgat acagactttt catgtgcatc aagcttttgt tgggacagat
      361 ggttacgata aagagatggg ctttacagga aaagatttaa tgcgcagtga ggtagttcaa
      421 tatatttcag cagcgtcgga taaagtcatt gtactaactg actcaagtaa atttgataaa
      481 aggggtacag taagaagatt tgctttaagt caagtctatg aagtaataac agacgaaaaa
      541 ctttctaaac aaaatatagc tacattagaa aatgctggga taatggttaa ggtagtttcg
      601 taagaggtta agtgtatgaa tcaagatagg aataaactgc tctctaaaat tgcttatctg
      661 tattatattg aaaacttaaa tcagtcacaa atagcagcaa aattaggaat ttatagaacc
      721 tctattagta gaatgttaac agaagcaagg aatgcaggaa ttgttaaaat tgaaatagag
      781 aattttgata ccaatatgtt taagttggaa aattatgtaa aagaaaaata tggtctggaa
      841 agtttagaaa ttattccaaa tgaatttgat gataatccaa caattttatc tgaaagaatt
      901 tctcaagttg cagcaggtgt ccttaggaat ctaattgatg ataatatgaa aattggcttt
      961 tcttggggga aaagtttaag taatttagta gatttaattc acagtaaaag tgtccgaaat
     1021 gttcacttct atcctctagc aggtggtcct agtcacatac acgctaaata ccatgtgaat
     1081 acactgattt atgaaatgtc tagaaaattt catggagagt gtacatttat gaatgcaacg
     1141 attgtgcaag aaaataaatt gttagcagat ggtattttgc aatcaagata ttttgaaaat
     1201 ttgaaaaata gttggaaaga tttagatata gctgtagtcg gaattggtga ttttagcaat
     1261 aaaggaaagc atcaatggtt agacatgctt acagaggatg actttaaaga attgaccaaa
     1321 gttaaaactg taggagaaat ttgttgtcga ttttttgatt caaaaggtaa agaagtctat
     1381 gaaaacttac aggaaagaac gatagcaatc tctttggaag atttaaaaaa tattcctcaa
     1441 agtttagctg ttgcttacgg tgatacgaaa gtatcttcga ttctttctgt cttgcgtgct
     1501 aatttagtaa atcatttgat tacagacaaa aatacaattt taaaagtttt ggaagaagat
     1561 ggggatttga cttttagaga gattctaggt gagtgaaaat gatagactga ttcagtttat
     1621 catttttctt tttagttgat tgcacatttg tgcttatata aacaaaaata gtttatctgt
     1681 tgtttttgga ttgacaactt
//
LOCUS       DAVKGC010000174         1650 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVKGC010000174 DAVKGC010000000
VERSION     DAVKGC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.174"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            123..506
                     /locus_tag="VW049_002019"
     CDS             123..506
                     /locus_tag="VW049_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9433064.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKPLENFN
                     HIAWFPIDEAIKNLKRGSHKWAIES"
     gene            695..994
                     /locus_tag="VW049_002020"
     CDS             695..994
                     /locus_tag="VW049_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9433065.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1255..1578
                     /gene="tnpB"
                     /locus_tag="VW049_002021"
     CDS             1255..1578
                     /gene="tnpB"
                     /locus_tag="VW049_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9433066.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          565 a          327 c          286 g          472 t
ORIGIN      
        1 aagattatac cacattgtgt actatttttg gttcatttca ctataataat aacgatacta
       61 gataatgagg aaaatgcaat ggcaaaccca actttcggag aaaaaaagcg aatacagact
      121 atattgctcg ctatggcgta tatgcagtta tccctaatcc tgaacaaaaa caaattgttc
      181 ttgttcaaga accaaatggt gcttggttcc taccatgtgg agaaattgaa gcaggtgaaa
      241 atcatcagga agccctaaag cgtgagttga ttgaagagct tggtttcaca gcagaaattg
      301 gtacctatta cggacaagct gacgaatatt tctattctcg tcatcgtgat acctactact
      361 acaatcctgc ctacctctat gaagcaactc ctttcaaaga agtacaaaag ccactagaaa
      421 actttaatca tattgcctgg ttccctattg acgaggctat caaaaacctt aaacgtggta
      481 gccataaatg ggccattgaa tcttgaaaaa aacagcataa gattgactaa tactcttcga
      541 aaataaaatt caaaccacat cagcgtcgcc ttaccatact caagtatagc ctgcggctag
      601 cttcctagtt tgctctttta ttttcattga gtataaatca agcgacttta ttcaaaaagt
      661 gctataatag aattagaaaa tcggaggaaa tattatgaag cttatcaata cgacaaactc
      721 acactcgcaa cttgtaaaaa gtcagctaga aagtaccgat gcaacccttg ttgaggttta
      781 ttctgcaggg aatacagatg ttatttttac ccaagctccg cttcactatg aaatcctcat
      841 ctcaaacaaa caccgtgcta ttcgtgagcc tgaaatcgaa accattcaag aatttttctt
      901 gaaacgtaaa attgataagg cctctgttga tgaagccaat atcaagacac tctactcaga
      961 gaaattaatt ggaatttcaa tcccaatcaa gtaagaaaat tgagtaaaaa ctcgatttga
     1021 gagaaagtcc aataagtctt tccataataa aacgcatagt agcaagagtt tctacaccca
     1081 ctattatacg tttttatcat tttgaaatct gctgctaatc aatgaagaag caaagatcaa
     1141 actaggcagc tagttacaag ttacgtaagc gcgtcataac aaggtatcta tcctaattcc
     1201 ccaacttcca gtatactaga taaaaaataa ttatcggagg aaaagtattg tactatgatg
     1261 attcaactaa gtgatttagg tcaagttcac cttgtttgtg gcaagacaga tatgatacaa
     1321 ggaatcgatt cactggctta tctcgttaaa acccactttg aattggatcc tttctccggt
     1381 caagtctttc tcttttgtgg tggacgtaaa gaccgcttta aagcccttta ctgggatggt
     1441 caaggatttt ggatactata taaacgcttt gagaacggaa aactgacttg gcccagtaca
     1501 gaaaaggatg tcaaagctct cgcacctgaa caagtagact ggcttatgaa gggcttttct
     1561 atcactcccc aaatatagtg gatttaaact agaatagtac acccctactt ctaaaacatt
     1621 gttagaaatc gatttgactg tcctgatcaa
//
LOCUS       DAVKGC010000175         1539 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVKGC010000175 DAVKGC010000000
VERSION     DAVKGC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.175"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..326
                     /locus_tag="VW049_002022"
     CDS             <1..326
                     /locus_tag="VW049_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433067.1"
                     /translation="IYFYSQVKVVDKLTDSESKKYGEYIDLYESCRQNNLSVLLNFLQ
                     NSDDKRGVIASNSVRIMFESLDKADQIAALNICRNYVARENVLNKEDAEKLLNEIDKK
                     LEISR"
     gene            382..1077
                     /locus_tag="VW049_002023"
     CDS             382..1077
                     /locus_tag="VW049_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9433068.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            1089..1505
                     /locus_tag="VW049_002024"
     CDS             1089..1505
                     /locus_tag="VW049_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9433069.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
BASE COUNT          476 a          237 c          373 g          453 t
ORIGIN      
        1 taatctattt ttattcgcag gtaaaagttg tagataaact tactgattct gaatcgaaaa
       61 aatacggtga atatattgat ttgtacgaat cgtgtcgtca gaacaattta agtgttctac
      121 ttaactttct tcaaaattcg gatgataaac gaggagttat tgctagtaac tcagtgagaa
      181 ttatgtttga atctttggat aaagcagacc aaattgcggc cctgaatatt tgtagaaact
      241 atgtagcacg agaaaatgtt ttaaataaag aagatgcaga aaaattgtta aatgaaattg
      301 ataaaaaact tgaaattagc agataggaca attcaaatag agttgagtaa aaagtttgaa
      361 aagtgaaaca aggagaaagc tatgccagta aatgaatatg gtcagatgat tggtgagtca
      421 atggaaggtt atacacccgg tgcactgcct tctattgatt tcttagaagg gcgttatgct
      481 aggatagagg ctctctcggt agaaaaacat gcggaggatt tgctagctgt ttatggcccg
      541 gatactcctc gggagatgtg gacctatctt tttcaggagc cagtagcaga tatgggggag
      601 ctggttagcc ttttaaatca gatgttggct cgtaaggacc gtttttacta tgcaatcata
      661 gacaaggcga ctggtaaggc tttgggaact ttttctctca tgcgcattga ccagaataac
      721 cgagtaatag aagtcggagc agtcactttt tctccagagc taaggggtac acggatagga
      781 actgaggctc agtatctcct agctcgctat atttttgagg agctaaacta tcgtcgctac
      841 gagtggaaat gcgatgcctt aaatctgcca tccagacgag ttgcggagcg tttgggattt
      901 gtctatgaag gaaccttccg ccaggcggtg gtttataagg ggcgtacgag agatacggat
      961 tggttgtcta tgattgatat ggactggcct caagtcaaag atcgtttgga aacatggctg
     1021 cgtcctgaaa attttgataa aaatggacag cagtacaaga gcttgagaga actttaagag
     1081 gtgttgagat gattactatt aaaaagcaag aaattgtcaa gctagaggat gttttgcatc
     1141 tctatcaggc tgtcggttgg acaaattata cccatcaacc agagatgctg gagcatgcct
     1201 tatcgcattc attagtaatt tatctggcac ttgatggtga tgctgtggtg ggcttgattc
     1261 gtttggttgg agatggattc tcatcagttt ttgtacagga tttgattgtt ttaccaatct
     1321 atcagcgtca agggattggt agtgccctaa tgaaagaggc cttaaaagat tacaaagatg
     1381 cttatcaagt ccagctagtg acagaagaga cagaaaaaaa cgtgggattt tatcgttcta
     1441 tgggctttga aatcttatcc acctataatt gtatagggat gacttgggcg aatcgagaaa
     1501 aataacaaaa cttgtttttt cttaagcaaa atttaagga
//
LOCUS       DAVKGC010000176         1509 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVKGC010000176 DAVKGC010000000
VERSION     DAVKGC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.176"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            27..1373
                     /gene="gdhA"
                     /locus_tag="VW049_002025"
     CDS             27..1373
                     /gene="gdhA"
                     /locus_tag="VW049_002025"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9433070.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          465 a          296 c          324 g          424 t
ORIGIN      
        1 aaatatgaca ataaaaggag aacgatatga catctgctaa agaatatatc caaagcgtgt
       61 ttgaaactgt aaaagctcgt aacgggcacg aggctgaatt cctccaagct gttgaagaat
      121 ttttcaacac tttggaacct gtatttgaaa aacaccctga gtatatcgaa gaaaatatct
      181 tggcacgtat tactgagcct gagcgcgtga tttctttccg tgttccttgg gttgaccgtg
      241 atggaaaagt tcaagtaaac cgcggctacc gtgttcaatt caactcagct gttggaccat
      301 ataaaggcgg acttcgtttc cacccaactg taaaccaagg gattttgaaa ttcctcggat
      361 ttgaacaaat ctttaaaaac gtcttgactg gacttcctat cggtggaggt aaaggtggat
      421 cagacttcga tcctaaaggt aaaacagatg ctgaagtgat gcgcttctgc caaagcttca
      481 tgactgaatt gcaaaaacac attggaccat cacttgacgt acctgctggt gatatcggtg
      541 ttggtggacg tgagattggt tacctttacg gtcaatacaa gcgtcttaac caatttgatg
      601 ctggtgtctt gactggtaaa cctcttggat ttggtggtag cttgattcgt ccagaagcaa
      661 ctggttacgg tttggtttac tatactgaag aaatgctcaa agctaacggt aacagctttg
      721 ctggtaagaa agtggttatt tcaggttctg gtaacgttgc tcaatacgct cttcaaaaag
      781 caaccgaact tggtgcaaca gttatctctg tttctgactc aaatggttat gtcatcgatg
      841 aaaatggtat cgacttcgat cttttggttg atgttaaaga aaaacgtcgt gctcgtttga
      901 ctgagtatgc agctgaaaaa gcaactgcaa cctaccacga aggtactgta tggacttacg
      961 ctggaaacta tgacattgct cttccatgtg ccactcaaaa cgaaatcaac ggtgaagcag
     1021 ctaaacgttt ggttgctcaa ggcgttatct gtgtatctga aggtgccaac atgccgagcg
     1081 accttgatgc catcaaagtt tacaaagaaa atggtatctt ctacggacct gcaaaagctg
     1141 ccaacgctgg tggtgtagcc gtttcagctc ttgaaatgag ccaaaacagt cttcgcctct
     1201 catggactcg tgaagaagtt gatggccgtc tcaaagacat catgacaaac atcttcaaca
     1261 cagctaaaac gacttcagaa acatacggtc ttgataaaga ctaccttgca ggagctaaca
     1321 ttgctgcctt tgaaaatgta gcaaatgcca tgattgcaca aggtattgtt taagatttat
     1381 ttactcaatc ctcaatcact acgattgggg atttttagct tgaattaaaa aattggaaaa
     1441 tctaattaaa taaaatgaaa tgaaataaaa actgaacaaa ttgattgcgg aattcaaacc
     1501 aatttctaa
//
LOCUS       DAVKGC010000177         1508 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVKGC010000177 DAVKGC010000000
VERSION     DAVKGC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.177"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..1484)
                     /gene="pfbA"
                     /locus_tag="VW049_002026"
     CDS             complement(<1..1484)
                     /gene="pfbA"
                     /locus_tag="VW049_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9433071.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNV"
BASE COUNT          458 a          295 c          204 g          551 t
ORIGIN      
        1 acattttttg ttacagtaat atctgatact tttcctaaat attctgcact atcttttgca
       61 actcgtatcg cttttgtttt aggatcggca atattaaata tattttcggc gataaccacc
      121 tgtttattta aatctaatag gtctttagtg tttttatagc tataagcatt tactaaagct
      181 gctccgcttt ctcgataatg tacactctct cctttaactt tcttatcaaa gcgatttcct
      241 ttgattaata catcagtgaa tcctgtaaaa cgtacacctg catacatcat gttatcaaaa
      301 tgattattta aaattttaat attagagggg ttctgtgtcg acaatgtttg atagtgtgtg
      361 ccaattgctg ttactaattc cccagattta tcacttttgc caaaatagga attttgaata
      421 gtcacatttt cagatttttt cccatcatca ttcaaggcat aaggaaaacc ttttctagtt
      481 aatggttcaa tctgaatgct ctccttactt atgatttgcc catccttcat cgttttgggt
      541 aaggcttgcc caagaaaacg agaattatca actaatacat ttttcgaacc tgcaatttga
      601 atagcatgcc cttgataact atccttgaat gttacatttt ttatagttac gttatttgaa
      661 ttcccaatag caaatgcacc tgaagaattt ataagtggta gattttttgc tttagttcct
      721 tcttcattca aagcaccgtt catatcaatc gtaccaccag aataactaat atcttctgtt
      781 gggccccatt ctacttgcgc accatcatcc gtaaataaac ctgtcataaa aacaatggaa
      841 gggtgattct taatatttat accatttaga attgtagctt tctcattcaa ttctaagtgt
      901 gtatgacttt ttaaaaaaac aatttctttt actaaataag ttccttcagg aaaatataca
      961 tttcctccac ctagcccttg agctgcagca tctattgcat cttgaattgc ttgtcgatca
     1021 tcatgaatcc catcacctac cgcaccataa tcttttacac ttaaagcagt atcttcaaat
     1081 tcttttttga taattctgtc acttataaca tcttccttat aactcttatt ttcattagta
     1141 gatgttactg tagattcaac tgttctgttg tctaattgtg aattggaacg aatagtacca
     1201 ttagagctga tactattaac acttaacgaa ttatctaaac cattagtggt tgaattagag
     1261 gcaatcattt cagcagaacg attctgatta acagttggtg aattatctaa atcattagta
     1321 atagcattag aagactcttt tgtagccatc ggtgaagaac tagtaacaac ttcatccgat
     1381 aaaatacctt ggcttcccaa aattgaaatt gccaatagta ccgagcaagt ccccaccttt
     1441 aatttacgaa tactgaatac ctcattagga acaaaatatt tcatcagaac ctccaatttt
     1501 ttactatt
//
LOCUS       DAVKGC010000178         1482 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVKGC010000178 DAVKGC010000000
VERSION     DAVKGC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.178"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            154..1332
                     /locus_tag="VW049_002027"
     CDS             154..1332
                     /locus_tag="VW049_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9433072.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NELIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          546 a          264 c          215 g          457 t
ORIGIN      
        1 gatttgtgta aatatttgat tattttttat tatcaatata taataaaaag agaccagtaa
       61 gcaatgaaaa gcttaaaaag attaacatct acatttatcc atagcaaaaa atcataggat
      121 tttaagaatc tgacaactag gaagaaaact aatatgacct tatctaagaa acaattacaa
      181 ctacgagcta aaatactgga aacagtttac acattaggtc ccatttcacg aattgaaatc
      241 gctactaaaa caggaataac acctgcaaca actagcagta tcacaaatga gctgattaaa
      301 gaaaacattc tcttagaact tggtgaagac gaacacgata caagcgtagg gagaaaaaaa
      361 atcctgctag acattcaagc taaacgattt tactacattg gttgtgaact atctgaaaaa
      421 catttcactt ttgctttagg agataatcta ggaaatatct taaaggaaga gaaagaaatt
      481 gttactaaac agttaatcca agaaaaggga aatcaactca ttaaccagac actaaagcaa
      541 ttcttaaata actgctctga ttatgaaata gaagcaattg gaatcgcgct acctggtcgc
      601 tatttagatg attataaaat aacaacaaac aatcctttat ggcaacatat cgacttagaa
      661 atgattcaat cccactttga taagcctttg tttttctcta acaatgttaa ctgtatggct
      721 ataggtaaac gcctatttag tcgccaacag aatgacccta attttgctta ttttcatttt
      781 gctagaggta tgcactgttc ttatatctac gacggaaaca tttatgggaa aggaaatcta
      841 atgattggag aaatagggca tacagttgtt tcatctgagg gagaagaatg tagctgcggg
      901 cgcaagggat gcttacaaac ttttgctgga gaatcttggt taatcaaaaa atcaaaaatt
      961 ctatatcatc aatctcccta ttctctactt cctagtttag tcaaaaatgc cgatgatatt
     1021 gatatccaag ttattttaac cgcttatcaa ttaggtgaca ctggaataat cacacttatc
     1081 catcaagcct tactctatct tagtcaaacc attttaaata tcagtatgat gattgactct
     1141 caaaaaatct acctacatag tcctttacta acaaatcaac acatcatcca aaaactatat
     1201 agtgaaatga actacaaacc caaactgctc tataatcgcc tacctgaagt cattattgaa
     1261 ccttacaatg attttacagc cgcacactca gctattgcac tttgtttgta tcatacaatt
     1321 ctacattctt aattttctat tacatttaat aaataggcta aaaagaggga gtatatcata
     1381 aaaagcgaat aaacctgttt tctgaaaatc agaattctgt tttgttcgct ttctttatat
     1441 ttggaattat actcctatct cccctttttc ataatgtata gt
//
LOCUS       DAVKGC010000179         1382 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVKGC010000179 DAVKGC010000000
VERSION     DAVKGC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.179"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(237..1346)
                     /locus_tag="VW049_002028"
     CDS             complement(237..1346)
                     /locus_tag="VW049_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9433073.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT          513 a          254 c          203 g          412 t
ORIGIN      
        1 tagaggtgta ctattctagt ttcaatctac tataaatagg tttcattcga actttaatag
       61 actatgaaaa ccagtggaac ttaccctgac acagaattcc actggttttt tcgattttta
      121 tcagattaac ttctactatt cttagctgtg aaaattagtt taagacttta acttattttc
      181 agtcttctaa tctaaaggcc acttccagta aagaaaaatc agggttctcc tgacttttac
      241 ttagctaatt ctctttctcg ttctttcatt attttatgat tttcatacaa acgggataag
      301 aacttagaaa tttctttcaa gatagaatag gttggcactg cgacaatcat tccaactaca
      361 ccatagatat tgcttgacaa caaaagtaaa actaaaatcg tgattggatg aaccttcata
      421 acacttccta cgattcgagg atataagata ttgccatcta cctgctgaac aacaagcata
      481 taaatcactg caatcagcag tctatgggga tcagtgaata tatttgcgat gatcatagga
      541 atcaaaccaa tacttggccc cacataagga attaaattgg ctacaccaga aaaaatggca
      601 aaaactaaag catattttaa accaataata ctatagccaa tataagccaa acaacctata
      661 atgattgcgt caatcgaaac tccactaata tagcgagcaa tcgtcgcatt taaattcttt
      721 aataagcctg caatatgcaa gcgatccctc tttagaatcg ttctttcaag catgggcaag
      781 aatttatgtc catctaataa gaaataaacc aaaaaaactg gagtcataat caaaatcaaa
      841 acagtactga taagagctga caagacgctc cccacactat ttgatacgct atttaggata
      901 ttttgaagaa tatcaacata ggataagttt aactgctgaa ttgtagcttc tacatccaaa
      961 ttctggagcg caggataatt agataagtct atgattaagt cttgtactcg actataaata
     1021 gtttgactag atataatcaa actagataac tgattaatca aaataggtaa gagatagaca
     1081 acacctatga ccattcccca gaccaaagta cacaaggtaa ttaaaatacc aagcaaacga
     1141 ttgagtttac agactttatt taagaaagta acaatagggt ttgtcaaata ataaagaaag
     1201 ccccctaata aaaatggaat cataattgta ttaagcacgc taacaaaagg gttaatcaaa
     1261 gatcccatct gtctccatag gtaaaagatg atggttaaga gtaaaatttc tgtggtccaa
     1321 aaaaataatt tatttctacg aaacatgagt tacctccctc actttatttt accatatttt
     1381 ca
//
LOCUS       DAVKGC010000180         1372 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVKGC010000180 DAVKGC010000000
VERSION     DAVKGC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.180"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(171..311)
                     /locus_tag="VW049_002029"
     CDS             complement(171..311)
                     /locus_tag="VW049_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433074.1"
                     /translation="MKFFWGLLAIIFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(308..490)
                     /locus_tag="VW049_002030"
     CDS             complement(308..490)
                     /locus_tag="VW049_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433075.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDNAVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(492..1271)
                     /locus_tag="VW049_002031"
     CDS             complement(492..1271)
                     /locus_tag="VW049_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433076.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT          448 a          299 c          207 g          418 t
ORIGIN      
        1 atagaactga cgaagtcagt aacatatata ctgcaaggtg aagctgacgt ggtttgaaga
       61 gattttcgaa gagtataaaa tgattttact gcttatatta taccatatgg agagagatta
      121 aaaaactagc agtggagttc ctgctagttt tctgcgactt ggattaccat ctagataagt
      181 ttaaagaacc aatccaatat cttgtaaaac agcaattgaa ctgcaaaaga aatgatcatc
      241 caaaagaatt tcacaatccc aataatcggt ttgataaaaa taatggcaag aagtccccag
      301 aaaaatttca ttctctctcc tctggcttaa tgagccaccg agccgtatcc attaacttgt
      361 cggcaataaa aagtttcccc agaccgacaa cggcattatc atctttcttt atcgttttca
      421 ttacttttac accttgcacg gtcaaaaagt aattaccata aattttagct agagttttta
      481 tgagtcccat attactctcc ttcgatgatt tcagcctctt ttcggattgt ttcaacatct
      541 tcttgatatt gaacttcact atagttaact agcttggaca attctttctg caagctttca
      601 cccatcggtt tcatagttgc aaaggttaaa ccacctgaaa tgataccacc caagatagga
      661 ataaatttcc ccattccttt ggccagccct cccttggtaa gattcacacc aaatattctt
      721 aagacttttt tcaaaatagg ataccaaagc gtctttgtta aagctttatt aggtactgtt
      781 ttcattacct gtttggcaat tgttatacca ccagcacgta gcaaagcagc agttccattc
      841 acccccaaca tgacgcctag ataaagcaaa agggtatttt gagcatcttc actcaactca
      901 tcgcgtgaag cccaaagatc ctcataacca taaatataac ctaattcttg ggccaatttc
      961 agagagaaac cataaaattg agccacatca gctggaatgg taattgccat agcaagccct
     1021 ccaggtaatc ctgccaaaac agaagtccca ctcgctaata aaacattatc cctaatacag
     1081 gcattagcca ctcgatctaa gatttcttga gataagagag acgttggacc ttgttccaat
     1141 aaggtaggaa tgtcctgtgg acccaattct ttggaaaact tatccactaa aaatttcgaa
     1201 cgatcaacct taacaactgg tagttttacc acttgttgta atacttgcat agctaagtct
     1261 tgttcagcca tatacaaact ccttatttct tttttacacc ttatcatatt atatttccta
     1321 caaaaaaagc attttctaat tagataacat taaaaaatac agaacccctt tt
//
LOCUS       DAVKGC010000181         1355 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVKGC010000181 DAVKGC010000000
VERSION     DAVKGC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.181"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..121
                     /gene="blpO"
                     /locus_tag="VW049_002032"
     CDS             <1..121
                     /gene="blpO"
                     /locus_tag="VW049_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9433077.1"
                     /translation="VMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDGCATTV"
     gene            225..344
                     /locus_tag="VW049_002033"
     CDS             225..344
                     /locus_tag="VW049_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9433078.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            812..1183
                     /locus_tag="VW049_002034"
     CDS             812..1183
                     /locus_tag="VW049_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433079.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
BASE COUNT          410 a          168 c          277 g          500 t
ORIGIN      
        1 tgttatggat aatgaaatgc ttgcttgcgt tgaaggtgga gatattgatt ggggaagaaa
       61 aattagttgt gcagcagggg ttgcatatgg cgcaattgat gggtgtgcaa caacggtttg
      121 acatttctat tgggaccatt tgctatagga ataggtgtaa ctggtgctgc aggtggagct
      181 atacttggag gtgtagccta tgcagcaaca tgttggtggt aattatggat tttaaaagtt
      241 ttattattgg tttagtagtt ggtatatttg gtccttatat ggatgactta attagaaaaa
      301 tattttcaaa atcttccaaa aaggatacgg atagtactct ctaaaaaata aatttctccc
      361 ctgtatttgc tagcttaatt tacaggggag tttctttaat attgtttcgc ctgtcaaaat
      421 gatatgtgat agcaggatga aaagtctaca aacaagttgg tatcaataca atataaagag
      481 gaaagtatgt ttttcatgct tgctttttta atttttacaa ttcaagaggt tttgatgacc
      541 atttatgatt tgagtgatcc aaggtcaaaa taagtgctat actagcagtg taaatgttct
      601 tgctcaacta agataaatcg cacatttagg aggaaaactt atgaagaaaa aaatactgat
      661 tattttcgtt ctgtatctga tcatgtccat ctttctttat ccgcttaggg agagtatttg
      721 gtataatcta ttttatacca tagcctatat gattgcggtt atgatctatt tttctttaat
      781 taaaaagaaa gaaaagaaat gaaagatttt cttgattaaa aagaatcata ttctttgtga
      841 gattgttcct gggtcagttg cctttgcttg tttctactta tctatttcta tttcgtcagt
      901 tttccaattt cttttagtag ttattaactt gacttctatt ttggttactg tttatcttac
      961 tagggaaatg agattaagag agtttgaaga tgatgatttg gttagtccta gaaccaatca
     1021 actcatgttt atcggcttga caggctttat gtctattatt tgtttgtata gaggtatcac
     1081 agcaggagaa tcttatcaac aactaatcgc ttatattggt gctattcttt gcttgatcat
     1141 catgcttcta ctcatttggg gtttgaagta ttataaaaag taggggctaa tgaagttttt
     1201 gcaatttagg aggtttcgat gacaattcaa gatttggatg aaaaatttta aaaaacaatg
     1261 atatactaaa cttgtcaaag ttgcaacggg tgctgctaca ttatgttaaa aatcgctctt
     1321 gattgataag ttggatttgt taggaaagga gtaaa
//
LOCUS       DAVKGC010000182         1354 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVKGC010000182 DAVKGC010000000
VERSION     DAVKGC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.182"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..1330
                     /locus_tag="VW049_002035"
     CDS             <1..1330
                     /locus_tag="VW049_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9433080.1"
                     /translation="YVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGTKKT
                     DLPSSDREFYNKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVEDI
                     LAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYV
                     TPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVK
                     AAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLL
                     ATVKYYVEHPNERPHSDNGFGNASDHVRKNKADQDSKPDEDKEHDEVSEPTHPESDEK
                     ENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKV
                     TDPSIRQNAMETLTGLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
BASE COUNT          463 a          256 c          295 g          340 t
ORIGIN      
        1 ttatgtcttt gaggagaatg gagtttctcg ttatatccca gccaaggatc tttcagcaga
       61 aacagcagca ggcattgata gcaaactggc caagcaggaa agtttatctc ataagctagg
      121 aactaagaaa actgacctcc catctagtga tcgagaattt tacaataagg cttatgactt
      181 actagcaaga attcaccaag atttacttga taataaaggt cgacaagttg attttgaggc
      241 tttggataac ctgttggaac gactcaagga tgtctcaagt gataaagtca agttagtgga
      301 agatattctt gccttcttag ctccgattcg tcatccagaa cgtttaggaa aaccaaatgc
      361 gcaaattacc tacactgatg atgagattca agtagccaag ttggcaggca agtacacaac
      421 agaagacggt tatatctttg atcctcgtga tataaccagt gatgaggggg atgcctatgt
      481 aactccacat atgacccata gccactggat taaaaaagat agtttgtctg aagctgagag
      541 agcggcagcc caggcttatg ctaaagagaa aggtttgacc cctccttcga cagaccatca
      601 ggattcagga aatactgagg caaaaggagc agaagctatc tacaaccgcg tgaaagcagc
      661 taagaaggtg ccacttgatc gtatgcctta caatcttcaa tatactgtag aagtcaaaaa
      721 cggtagttta atcatacctc attatgacca ttaccataac atcaaatttg agtggtttga
      781 cgaaggcctt tatgaggcac ctaaggggta tactcttgag gatcttttgg cgactgtcaa
      841 gtactatgtc gaacatccaa acgaacgtcc gcattcagat aatggttttg gtaacgctag
      901 cgaccatgtt cgtaaaaata aggcagacca agacagtaaa cctgatgaag ataaggaaca
      961 tgatgaagta agtgagccaa ctcaccctga atctgatgaa aaagagaatc acgctggttt
     1021 aaatccttca gcagataatc tttataaacc aagcactgat acggaagaga cagaggaaga
     1081 agctgaagat accacagatg aggctgaaat tcctcaagta gagcattctg ttattaacgc
     1141 taagatagca gatgcggagg ccttgctaga aaaagtaaca gatcctagta ttagacaaaa
     1201 tgctatggag acattgactg gtctaaaaag tagtcttctt ctcggaacga aagataataa
     1261 cactatttca gcagaagtag atagtctctt ggctttgtta aaaaaaagtc aaccggctcc
     1321 gatacagtag taaaatgaat ggagcatatt ttat
//
LOCUS       DAVKGC010000183         1330 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVKGC010000183 DAVKGC010000000
VERSION     DAVKGC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.183"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            1..443
                     /locus_tag="VW049_002036"
                     /pseudo
     CDS             1..443
                     /locus_tag="VW049_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
     gene            552..1199
                     /locus_tag="VW049_002037"
     CDS             552..1199
                     /locus_tag="VW049_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9433081.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KRTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(1188..>1330)
                     /locus_tag="VW049_002038"
     CDS             complement(1188..>1330)
                     /locus_tag="VW049_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9433082.1"
                     /translation="ERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHEL
                     GF"
BASE COUNT          505 a          179 c          254 g          392 t
ORIGIN      
        1 taatattcca gaaaatttaa aagataagag agttgatgaa gttgatagaa ctccagcaga
       61 aaaccttgct tatcaggttg gctggaccaa cttggttctt aaatgggaag aagatgaaag
      121 aaaggggctt caagtaaaaa caccatcgga taaatttaaa tggaatcaac ttggtgaatt
      181 tatatcagtg gttcacagat acctacgctc atttatctct gcaagagttg aaagcaaaat
      241 taaatgaaaa tattaattct atctctgcaa tgattgattc gttgagtgag gaagaattat
      301 ttgaaccgca tatgagaaag tgggctgatg aagcgactaa aacagcgact tgggaagtgt
      361 ataagtttat tcatgtaaat acggttgcac cttttggaac tttcagaact aaaatcagaa
      421 aatggaagaa gatagtatta taaattatat ttttaacttt aaaaaatttc ataaaaatgg
      481 ttaccaaagg cgatagaagc aaaaactatc gtcttttctt tgcaaatttt taagaaggga
      541 ggtgatcttg catggacttt gaatattttt ataacagaga agcggaaaga tttaacttct
      601 taaaagtacc ggagatatta gttgatagag aagaatttcg gggcttatca gcagaagcaa
      661 ttatccttta ttccatactt cttaaacgga caggaatgtc atttaagaat aactggatag
      721 acaaggaagg cagagtattt atctatttta ctgtcgaaga aattatgaaa agaagaaata
      781 tctcaaagcc aactgccata aaaacattag atgagcttga tgtaaaaaaa ggaataggac
      841 tgatcgaaag agtaaggctt ggacttggta agccgaacat catttatgtt aaagacttta
      901 tgagtatatt tcaggtaaaa gaaaatgact tacagaagtc aaaaaactta acttcagaag
      961 taaaagattt taacctcaga agtaaagaaa atgaacttca agaggttaag aaccttgact
     1021 ctaactatat agagaataat aagagtaagt atagtaagag agaatatagt tttggtgaaa
     1081 acggacttgg aacatttcaa aatgtgtttt tagctgctga agatatatcg gatttacaaa
     1141 tcataatgaa ctcacagctt gagaattaca ttagacttcc tgcaaaacta gaatcctagt
     1201 tcatgattga taataccagc aatcaaattc attcgtaatc cgaagcgttt acgatgattt
     1261 cgataggttg ttgaaaacat tttaaacgtt tttactttgg caaagatatt ctcaaccttg
     1321 cttctctcct
//
LOCUS       DAVKGC010000184         1330 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVKGC010000184 DAVKGC010000000
VERSION     DAVKGC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.184"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..249
                     /locus_tag="VW049_002039"
     CDS             <1..249
                     /locus_tag="VW049_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433083.1"
                     /translation="LGIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIV
                     VINEFIACLNSRCMPSQYDRFFYKDRPYIFSRKSPIKG"
     gene            564..>1330
                     /locus_tag="VW049_002040"
     CDS             564..>1330
                     /locus_tag="VW049_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433084.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGVAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKL"
BASE COUNT          443 a          179 c          224 g          484 t
ORIGIN      
        1 ttgggaatca tttacgataa tgaagcatta agagatttgt caaaacaata tttaaacaaa
       61 aatatttcgg aattacaaga tggttctttc gagggcggta tacaagatgc tgttgcctct
      121 aaacaaatag tagttataaa tgaatttata gcttgtttaa attccagatg catgccatcc
      181 caatatgata gattttttta taaagataga ccttatattt tttccagaaa gagtcctatt
      241 aaggggtgaa aatttgttat atttggatta acaagtgtcc tagggattag aaataactct
      301 agtatagcta gatatttttg agtaactaga aatatcatta ggctactcat atgattgcca
      361 gaaaagatgg ctgatgaatc ttatttatac tgttgcagta agcattgtct aggataaata
      421 agctatcttg atgggggaga ttggaataat tttagtagaa acattaaagg attatgggca
      481 agtagcgcag gattaggtga tgcaatgtgc taagttggct tggtgttagc agtagtatta
      541 tttaacaatg gaaagtagac taaatgaaaa ataaacgtta tttttttgac acaattttaa
      601 tgattttact gcttataagc acgatatttt gtgtcagccc agtatttata aaattggaca
      661 tcttaggaac tccctctcat gccatcttaa cctttgtatt ggcgataccg ttattctata
      721 ttttgtctca atgcttacat actttgctat tactagtttc ctcaatcttt tgtaaattaa
      781 gaccaattta tttttatttt atatttgtga tcattatagg agctaggaaa tactatcgaa
      841 ttctctttca ccaactaatg ggatttagtc caggagtagc agtgttttat aaggagagtc
      901 aaacaactaa gaatctgttt aaattttact acttcttgta ctttacaaca ctaatttcat
      961 actatttttt ctttactttt gtgtatgaca aaccgttgct tttgcctctt attccatttt
     1021 ctattatcat agcattagta caaaaacttt atagaattga gaatcagcaa ttatttctgt
     1081 taaaatcaaa ggtactaaca atactggaat ctaagaagaa ttgtgagttt aatcttcagg
     1141 attatcatga aatttggaaa ttgcaatcaa aatctgagtt gccatgcgtg gctttatctt
     1201 atatcagttt aatcaagcca tatctttcag agagtgtgcg tgaacaaatt gatttattag
     1261 aagttaagcg atttaaaaaa ataaatcatc caatatcact ttatggtatg ttggatgtta
     1321 taaaattaaa
//
LOCUS       DAVKGC010000185         1324 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVKGC010000185 DAVKGC010000000
VERSION     DAVKGC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.185"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            328..768
                     /locus_tag="VW049_002041"
     CDS             328..768
                     /locus_tag="VW049_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9433085.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLIEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            765..1223
                     /locus_tag="VW049_002042"
     CDS             765..1223
                     /locus_tag="VW049_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9433086.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
BASE COUNT          430 a          295 c          246 g          353 t
ORIGIN      
        1 ctagaaattg attagtgcta ttataccata tttcagaaag aacagaaaag ctccaccaca
       61 taggagccca atccctccac atagacaaag taccttgcca gagggagtaa aatagtggta
      121 tactcaataa aaatcaaaga gcaaactaga aagctaggcg cagacagctc aaaacactgt
      181 tttgaggttg tagataaaac tgacaaagtc aacaactatc ctacggcaag acgacgctga
      241 cgtggtttga agagattttt gaagagtatt aataaaggtg tacaagaagt gagattaatt
      301 ttgtatgaaa aacaaggaga gaaatttatg aaagtagaat tacagattag tgagacttac
      361 gaggaggaaa agctgattgt ccaagcacct cagccgacag ataaagttca gaaagtcatc
      421 gagttcgcag aaaatctgga ccaaagagaa aaaatcaaag ggaaaatcga tgatcaggtc
      481 tatctagttg agattggcaa gattcagcgc ttctatatcg agaatcggaa ggttctagca
      541 gaaaccgcga gtcagaccta cagcattgat ttgcgactct atcaggttct taaactcttg
      601 ccaaccaatt ttatccaaat ttcccaatca gaaatcatca atatcgattc catctctcac
      661 ctcaagctca cgccaaacgg tctgatagaa attttcttga aaaacgaaag cttcacctac
      721 tcttcacgcc gttatctaaa aaccatcaag gagaaattag aactatgaaa aaacaagtat
      781 ttcacgatgc agctgccggt gttcttatcg gcctcatcct ctctatcctc ttttcactca
      841 tttatgcacc aaatacctac gcaccactaa atccctactc tctcatcggc caagtgatgg
      901 atcagcatca ggttcacggt gccctggtct tgctctactg cacacttatc tgggcaacca
      961 tcggtatgct cttcaacttt ggcaaccgct tatttagccg tgactggagc atgcttcgtg
     1021 ccactctgac tcatttcttc cttatgctgg ctggctttgt cccactagca actcttgctg
     1081 gttggttccc tttccactgg attttctacc tccagctcat tatcgagttt gcgattgtct
     1141 atctcatcat ctgggctatt ctctataaaa gagaggctaa aaaagtagat cacatcaatc
     1201 aactcttgga gcatagaaaa taggaacctc ataataagca aatccgagaa agaaacttct
     1261 cggtttttct ttatcctgct gcaccttact atagtgaaat gaaataagaa caggataaat
     1321 cgat
//
LOCUS       DAVKGC010000186         1289 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVKGC010000186 DAVKGC010000000
VERSION     DAVKGC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.186"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..187)
                     /locus_tag="VW049_002043"
     CDS             complement(<1..187)
                     /locus_tag="VW049_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433087.1"
                     /translation="MTIEEYNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRD"
     gene            complement(221..934)
                     /locus_tag="VW049_002044"
     CDS             complement(221..934)
                     /locus_tag="VW049_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9433088.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
BASE COUNT          424 a          250 c          210 g          405 t
ORIGIN      
        1 aatcccttct cccaatataa tccccttctt tgactaatga tggagatttc caagtaaaaa
       61 ttaaatcgcc agagaaattt ttcttatcaa aaaacttaaa aagagattga taaatactat
      121 ttgctaattg acaagtgtca gatgtaaatt cataattaac agcttcattg tactcttcaa
      181 tagtaatatt agcttttttg tagtcattag ctcgtctcct ttaaaatgtg atcttttcaa
      241 gaatattttt atctaaacaa tccagcaagt cttggtaaga atagacttcg taagtcggct
      301 gggcttgtgt gtgattttcg aggtgatgag gattatacca gatagtgtca atccccgcat
      361 tattgccacc ttgaatgtcg gcggttagag aatctccaat catcagcgtc ttttctttac
      421 taaatccagc aatttgctgg ccaatctttt cataaaagag agcatccggc ttttgagttt
      481 gcaactgttc tgagataaag acttgattga aataaggtac tagaccagat tgagccaaac
      541 gtcctgtctg aatggcagta atgccatttg tcgcagcata caagtcataa tcacgctcaa
      601 tgaggctgtc caagagatca tgagcgcccg atagtgtttg tccctgctga gcgagataaa
      661 attggtaacg ctgggcaaga aaactaccgt ctttttcctg tccaaaatga gaaaataaac
      721 gagaaaagcg cgtgttaacc aactcttgtt tactgatttt cttcagctcc aagtctttcc
      781 agagagcctt gttcatagga acgtaataat ctttataagc ctgaatatcc gcaactcctt
      841 cttcttttag aagttgggtc aaagccacat cctcagcagc atcaaaatca agaagagtgt
      901 ggtcgaggtc gaagagtaga aatttgtaga acaatttgag gttttccttt ctgaaaattc
      961 attaagaaca ttatatcata aagcacctca tacaattaac taatttaatc acttaaaaaa
     1021 attcgaacac tttctataca actgacagct caaatctttc agaatagaac aatactaact
     1081 atcgaacacc ccgtcttcat aaatacatat gtaattctag acctagaatt cctataaact
     1141 aaatgctttc atactcttcc aagtaattga ttgccttaaa ttttaatttt tgaaggattc
     1201 taaagctaga atagccccat cacaatcagt tttgattgat tcacaattta gaaacactat
     1261 agtttcactc ctgttaaaat aaaaaggaa
//
LOCUS       DAVKGC010000187         1286 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVKGC010000187 DAVKGC010000000
VERSION     DAVKGC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.187"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            87..>1286
                     /locus_tag="VW049_002045"
     CDS             join(87..426,426..>1286)
                     /locus_tag="VW049_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9433089.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYLKKFKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQAIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLK"
BASE COUNT          459 a          219 c          285 g          323 t
ORIGIN      
        1 ttcagatcta tgaactaaga aagcaaggac aaagcttcaa acagctttca aaaagatttg
       61 gtgtggatgt ttctggtcta aagtacatgg tgaaattaat tgaccgttat ggcatagaaa
      121 tcgttaaaaa agggaagaat cgtcactatt cttctaaatt gaaacaagaa atgatggata
      181 aagccttact ggaaggttgt tcacaaagaa gtattagtct tgattatgcc ctcccaaacc
      241 aaggaatgct ttcattttgg ccggcacaat acaagaaaaa cgggtatact attgttgaga
      301 aaacaagagg gagaccagct aaaatgggac gtaaacggaa gaagacttgg gaagaaatga
      361 cagaactaga acgactccag gaggagaatg aacgcttacg gactgaggtg gcctacctaa
      421 aaaagttaaa agagttagag gaaagggacg aagccctaga gcgagaaagg cagagacagt
      481 tagagaaatg gtttcaggag gatttcgact agattttctt cttgaaacag cacgtttagc
      541 tcgctcgact tactattatc agttgaaaca actggatggg gttgataaag ataaagagat
      601 taaaaccgaa attcaggcca ttgataatga acataaaggc aactatggct atcgtcgaat
      661 ccatttagaa ctaaggaatc gtggttttgt ggtcaatcat aagaaagttc aacggttgat
      721 gagaattctt ggtttaacgg ctcgaattcg ccgtaaacgg aagtattctt cctaccaagg
      781 agagattggc aagaaagcag agaatctcat tcaacgccag ttcgaagcat cgaggccaat
      841 ggaaaagtgc tatacagatg tgacagagtt tgccattcca aatagcacac agaaactcta
      901 tttatcacca gttttagatg gttttaacag tgaaatcatt gcttatcatc tttctacttc
      961 gcccaactta gaacaagtga agagtatgtt agaacaagcc tttacagaga aatactatga
     1021 gaatacgatt ctccatagtg accaaggctg gcaataccaa cacgattctt atcatcggtt
     1081 cctagagagt aagggaattc aagcatccat gtcacgcaag ggcaacagcc cagacaacgg
     1141 catgatggaa tctttctttg gcattttgaa atcggagatg ttttatggtt atgagaagaa
     1201 ctttagatct ttagaaaacc ttgaacaagc tattgtggac tacattgatt actacaacaa
     1261 caaacgaatt aaggtaaaac taaaag
//
LOCUS       DAVKGC010000188         1257 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVKGC010000188 DAVKGC010000000
VERSION     DAVKGC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.188"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(650..1222)
                     /locus_tag="VW049_002046"
     CDS             complement(650..1222)
                     /locus_tag="VW049_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433090.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
BASE COUNT          381 a          220 c          210 g          446 t
ORIGIN      
        1 tcttgtaata agcttgttac ctgataagat ttttggaaaa aataaaaaaa tttggaaaat
       61 agtttttgca atattgacgg cagtggcagc attgtcattt atgtactaag ttattttaag
      121 aatgtaggga aataaaccct acattctttt tagttttttc tgttttctaa attctattta
      181 tccaagcgct tcaacatttc ttgcttcttc gcttcaagtt ctgcacgctt ttcttcgatt
      241 tcggcatgtt ttttctcgag ttcagaacaa cttgcaccat tgctaaattc ttttcgccat
      301 caggagatag ggtgagtcga catgtctatt actcacccaa agcagtccta caaagcagga
      361 attttctgtt acttttttgg aaatagtaac gtttatacag ctttgacact tcgtatcaaa
      421 gcgccaaaca cactccgagg gatttacaga aagcagaaaa ggaatgatct ggtataagat
      481 cattcctttt ctctcttttt ctttaagtaa ttatatacaa tgtacgacga agtcgtcatt
      541 gcaatgctga tccaccacct aaagggaact ttaaacaaca ttgataagat aaagaatata
      601 aacaacgaaa atacgttata cccaattaat tttattgtat atctcatgat taaaagttaa
      661 tccttccgtt gttaggaatg gcatcatttt tatcccataa ttgtgctaaa taagtccccg
      721 gtgataataa attcatagcg gattctaaag caacatcatt tacaaaccaa ctacctagat
      781 atctagaaat tgctgaacga atagcacttt ttgctgcatg ttttcctttt actttaatta
      841 gatttgcaag gcctgcagta gttcctccta atgctaaagc tattgcagta tctaatagag
      901 cacccatttg attaactgta ataccttgcc aagctgctct aaatggagag tatgtaggtg
      961 ggattgtata atcgccttgt aattgtcggt taattacttc tttgatccat tgttgtgaga
     1021 cgtctggatg aaaagattgg atttcgtttg caagtgtatt gatttgttct tctgttagag
     1081 aagtgacagg ttgaagttcc atatttgttt caatttgtga tacttgttca gaagcgtata
     1141 cagctgaaac acttggaatc gctgatacaa ttaacacaat tgacgtcaaa aaaaccgaaa
     1201 taaatttcat taatttgttc atgagctttt ctccttttta tttgtatctg cttacat
//
LOCUS       DAVKGC010000189         1226 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVKGC010000189 DAVKGC010000000
VERSION     DAVKGC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.189"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            20..>1226
                     /locus_tag="VW049_002047"
     CDS             20..>1226
                     /locus_tag="VW049_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9433091.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QAAQKTENLTPDEVSKKEGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDATHADNVRTKEEINRQKQEHS
                     QHREGGTSANDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEERIARIIPLRYRSNHWVPDSRPEEPSPQLTPEPSPSPQPAPNPQPAPS
                     NPIDEKLVKE"
BASE COUNT          426 a          263 c          244 g          293 t
ORIGIN      
        1 cttaagaaaa gaggaaagaa tgaaaatcaa taaaaaatat ctagctgggt cagtagctac
       61 acttgtttta agtgtctgtg cttatgaact aggtttgcat caagctcaaa ctgtaaaaga
      121 aaataatcgt gtttcctata tagatggtaa acaagcagca caaaaaacgg agaatctgac
      181 tcctgatgag gtcagtaaaa aagaaggtat caatgccgaa caaatcgtca tcaagataac
      241 agaccaaggc tatgtcactt cacatgggga ccactatcat tattacaatg ggaaggttcc
      301 ttatgacgct atcatcagtg aagaattgct gatgaaggat ccaaactacc agctaaagga
      361 cgaggatatt atcagtgaaa tcaagggtgg ttatgttatc aaggtagatg gaaaatacta
      421 tgtttacctt aaggatgcaa ctcatgcgga taatgtccgt acaaaagaag aaatcaatcg
      481 acaaaaacaa gaacatagtc agcatcgtga aggagggact tcagcaaacg atggtgcggt
      541 agcctttgca cgttcacagg gacgctacac cacagatgat ggttatatct ttaatgcttc
      601 tgatatcatt gaggacacgg gtgatgctta tatcgttcct cacggcgacc attaccatta
      661 cattcctaag aatgagttat cagctagcga gttagctgct gcagaagcct tcctatctgg
      721 tcggggaaat ctgtcaaatt caagaaccta tcgccgacaa aatagcgata acacttcaag
      781 aacaaactgg gtaccttctg taagcaatcc aggaactaca aatactaaca caagcaacaa
      841 cagcaacact aacagtcaag caagtcaaag taatgacatt gatagtctct tgaaacagct
      901 ctacaaactg cctttgagtc aacgccatgt agaatctgat ggccttgttt tcgacccagc
      961 gcaaatcaca agtcgaacag ccagaggtgt agctgtccct catggtaacc attaccactt
     1021 tatcccttat gaacaaatgt ctgaattgga agaacgaatt gctcgtatta ttccccttcg
     1081 ttatcgttca aaccattggg taccagattc aagaccagaa gaaccaagtc cacaactgac
     1141 tccagaacct agtccaagtc cgcaacctgc accaaatcct caaccagctc caagcaatcc
     1201 aattgatgag aaattggtca aagaag
//
LOCUS       DAVKGC010000190         1163 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVKGC010000190 DAVKGC010000000
VERSION     DAVKGC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.190"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(63..877)
                     /locus_tag="VW049_002048"
                     /pseudo
     CDS             complement(63..877)
                     /locus_tag="VW049_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
BASE COUNT          345 a          203 c          237 g          378 t
ORIGIN      
        1 aaggaattag aaatgttgtt gctattaaat gaagtatgat atatattaga cttgttcgga
       61 atctagttca cttcataatt gatgatggca caaattaatt ccgcccgtaa ctcgaaacgt
      121 tttctgcggt tacgataagg gactgacatg atttggaagg tcttgaattt agcgttaaaa
      181 tgttcaattt caattcgtat cgctgacatc tctttattta actgcttatc atcctcactc
      241 aggcggcgat ttttggaatt tttagcagga atgaaagtat tctcatgaaa tttcaagatg
      301 cctaaataac ctaggtcaac aaaggcaagc gtcgtttcag gcaaactttg tccaatactt
      361 tctttgaaga gagtaaaatc atgcgtatgt ccgtcagaaa aggccatttg acagacttta
      421 tgtgtcgtca aatccagcat aatttgagtt tttaaggtgt gtcgtttctt tttaccagaa
      481 taatttttgc tttggttttg tttggacgct gaatcggact ttcggtcacg tcaatagcca
      541 cagcagcact cggggcttct aaatggtcca aatcaaagct acctgacgca cgaagtgtat
      601 cctccaccca agtgatgatg gcatttaccg tagctacacc gacgccaaaa tcaaaggcca
      661 gcagacgctg agtgggataa tatcgcaagt aacggagggt cataatgagc tggtcttcca
      721 tgggcagact acgtggacgt ccaccttttc ggtgctgaat ctgataggct gaattcaggt
      781 gttctagcat gagttgaaag gtttcctttt tgacaccaat caatagtttg aagttttttg
      841 agttaagttt ttgtgctttg tcgtatactg tttccataaa aatagtataa cactttttac
      901 agttaccgaa caagtctatt actccaatat gtcgtaagat agaaatgata tatataggta
      961 atggtatgta gggtaggaag aacaaactga ataataagat taaaattggt aaattagata
     1021 ttaatgctgc tcctaaattt ttccaataat ctttaaaaaa actgtttgcg aagtctctat
     1081 agggatagga ttcctcgtgt cgtacaattt ttattagagt tgctattagg gcactttcac
     1141 ctgatccgat agcaataact gtt
//
LOCUS       DAVKGC010000191         1155 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVKGC010000191 DAVKGC010000000
VERSION     DAVKGC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.191"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..703
                     /locus_tag="VW049_002049"
     CDS             <1..703
                     /locus_tag="VW049_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9433092.1"
                     /translation="SVIIGSNGCGKSTLIKTLSRLIKPLEGEVLLDNKSINSYKEKDL
                     AKHIAILPQSPIIPESITVADLVSRGRFPYRKPFKSLGKDDLEIINRSMVKANVEDLA
                     NNLVEELSGGQRQRVWIALALAQDTSILLLDEPTTYLDISYQIELLDLLTDLNQKYKT
                     TICMILHDINLTARYADYLFAIKEGKLVAEGKPEDILNDKLVKDIFNLEAKIIRDPIS
                     NSPLMIPIGKHHINS"
BASE COUNT          474 a          187 c          185 g          309 t
ORIGIN      
        1 atcagttatt attgggtcaa atggttgtgg gaaatcaaca ctcattaaaa ccttgtctcg
       61 acttataaag ccattagagg gagaagtatt gcttgataat aaatcaatta attcttataa
      121 agaaaaagat ttagcaaaac acatagctat attacctcaa tctccaataa tccctgaatc
      181 aataacagta gctgatcttg taagccgtgg tcgtttcccc tacagaaagc cttttaagag
      241 tcttggaaaa gatgaccttg aaataataaa cagatcaatg gttaaggcca atgttgaaga
      301 tctagcaaat aacctagttg aagaactttc tgggggtcaa aggcaaagag tatggatagc
      361 tctagcccta gcccaagata caagtatcct acttttagat gagccaacta cttacttgga
      421 tatctcatat caaatagaac tattagacct cttgactgat ctaaaccaaa aatataagac
      481 aaccatttgc atgattttgc acgatataaa tctaacagca agatacgctg attacctatt
      541 tgcaattaaa gaaggtaaac ttgttgcaga gggaaagcct gaagatatac taaatgataa
      601 actagttaaa gatatcttta atcttgaagc aaaaattata cgtgacccta tttccaattc
      661 gcctctaatg attcctattg gcaagcacca tattaactct taataattga atattggaat
      721 tcatttaaaa gtcgcaaatt ttgaactgac cctcaaaagt taggccaaaa aactaactaa
      781 aggaggtcag ttttttaatg gcaaatacag cacagaattt aagataaaat tagtaaaaga
      841 atatcatgaa tgaaaaatag gatatagaga ataacagcaa tattaaaaag atcaggactg
      901 ataataaaca aaaagaaagt acaacgacta gttcaaaagc taaaacttca agtaaaaagt
      961 tattcaagaa aatctagaaa atactcatcc tacaaaggac aggtaggaaa aatatcagac
     1021 aacaaaataa aaagaaatat ttattaccga aaaaaagggg aaatcgagtt aagtaccaac
     1081 aggctcatct attccaaaaa gatatttact cgatggaaaa actcctagaa ttagtgctac
     1141 aaatattgac aatgg
//
LOCUS       DAVKGC010000192         1139 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVKGC010000192 DAVKGC010000000
VERSION     DAVKGC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.192"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..425
                     /locus_tag="VW049_002050"
     CDS             <1..425
                     /locus_tag="VW049_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9433093.1"
                     /translation="IENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGE
                     QQKVAIARAVIADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELA
                     SKTDRALILKDGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            428..>1139
                     /locus_tag="VW049_002051"
     CDS             428..>1139
                     /locus_tag="VW049_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9433094.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQMSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVII"
BASE COUNT          355 a          146 c          231 g          407 t
ORIGIN      
        1 taatagaaaa tatagcactt cctttgcgat tatcacaaaa aaaattaact attaaaaatg
       61 tagaaaactt actcaaaaga atgaagttta atgctggctt aaacgatttt gttggaactc
      121 tgtctggtgg agaacaacag aaggttgcta tagctagagc ggttattgct gatagtgata
      181 taatatttgc tgatgagcca actggggctt tagacagcgt ttctcgtgaa gtaatttttg
      241 aattattgag agagttagta ggggcgggta agtgtgtaat tatggtaacg cacgatatag
      301 aattggcctc gaaaactgat cgtgcattaa tattgaaaga tggaaaaatt ttcaaagaac
      361 ttcatagacc tagcggggaa gagttgtata aaatcttaga ggtacaatca actacggagg
      421 aatagagatg ctcagattag tttactatca atttttacat aataagaaac aatggttagg
      481 cgtttcacca gtaatttttg tttcgagttt agttatggga ttggctgtta atggggtaat
      541 taatgttgag aataattcgc aagtatttgt cggacttcca gatcctaaac caatttttat
      601 gtttccgata gtattcggag gagtaacatt attctttgtg ctctctaaca taataaatat
      661 gttggttgaa atttttagag atgattacga acttctggag gtgttgggag caagtcgcct
      721 tcaactatca tttcttgtgg ggggacagat ttttatcatt tcctcgatta ttagttttat
      781 tgcatatcta tgctctatat ttgttacgtc aaattattat tattttttac aatacttttt
      841 cggggaaaat atattacctg atatccaatt tcaaatgagt gctgttggat gtatcattac
      901 agtagtgttg atttcatttt tagcctttct aagtggttgc ttttatacgt tcaagaagat
      961 tagaaatagg aagagttcta aaataaggca tgttctttca atagtaaaaa gaatactgct
     1021 attggctggt tttagtgtta tctggttgct ttctttgcaa caaattttcc aagatagcac
     1081 aattttagcg aaggcccaga taatattcaa tattgtaatc ttagatattg tgattattt
//
LOCUS       DAVKGC010000193         1105 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVKGC010000193 DAVKGC010000000
VERSION     DAVKGC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.193"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..978
                     /locus_tag="VW049_002052"
     CDS             <1..978
                     /locus_tag="VW049_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9433095.1"
                     /translation="FSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVAT
                     IAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNF
                     EAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKALGME
                     KEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANY
                     LTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKDPLLG
                     KINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
     gene            968..>1105
                     /locus_tag="VW049_002053"
     CDS             968..>1105
                     /locus_tag="VW049_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9433096.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MG"
BASE COUNT          430 a          156 c          189 g          330 t
ORIGIN      
        1 tttagcgctt gttcttctaa ttctgttaaa aatgaagaaa atacttctaa agagcatgcg
       61 cctgataaaa tagttttaga tcatgctttc ggtcaaacta tattagataa aaaacctgaa
      121 agagttgcaa ctattgcttg gggaaatcat gatgtagcat tagctttagg aatagttcct
      181 gttggatttt caaaagcaaa ttacggtgta agtgctgata aaggagtttt accatggaca
      241 gaagaaaaaa tcaaagaact aaatggtaaa gctaacctat ttgacgattt ggatggactt
      301 aactttgaag caatatcaaa ttctaaacca gatgttatct tagcaggtta ttctggtata
      361 actaaagaag attatgacac tctatcaaaa attgctcctg tagcagcata caaatctaaa
      421 ccttggcaaa ctttatggag agatatgatt aaaattgatt caaaagcctt aggtatggaa
      481 aaagaaggtg atgagttaat caaaaatact gaagctcgta tatccaaaga attagaaaaa
      541 catccagaaa tcaaaggaaa aatcaaagga aaaaaagtat tatttactat gattaatgct
      601 gcagatacat caaaattctg gatttatact agcaaagatc caagagcaaa ttatttaaca
      661 gatttaggtc tagttttccc tgaatcatta aaagaatttg agagtgaaga tagttttgca
      721 aaggaaattt ctgcagaaga agcaaataag ataaatgatg ctgatgtaat cataacttat
      781 ggtgatgata aaactcttga agctttacaa aaagatcctc ttttaggtaa aataaatgca
      841 attaaaaatg gtgccgttgc tgtaattcca gataatacac cgttagcagc ctcatgcact
      901 ccaacaccac tttcaataaa ctatactatt gaagaatacc taaatctttt aggaaatgca
      961 tgcaaaaatg cgaaataaaa aacaaataaa cctaggcata atttttgtaa tctgcctagg
     1021 tcttcttatt acaatatttt tgtcattaaa gcttggaaca aaagaaatta atatcagaga
     1081 ttttttagca gcttttggaa tgggt
//
LOCUS       DAVKGC010000194         1078 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVKGC010000194 DAVKGC010000000
VERSION     DAVKGC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.194"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(121..915)
                     /locus_tag="VW049_002054"
     CDS             complement(121..915)
                     /locus_tag="VW049_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9433097.1"
                     /translation="MLETTSTVIKVTIPMIAIFLYKLLGIHGVLLLDGLSFLIAASLI
                     FFITPVNEEVDTKENMTIRGIFDDLKIGFTYVYSHKQILTIIALSALVNFFLAAYNLL
                     LPYSNQMFGSISSGLYGTFLTAEAIGGFIGAILSGFINKSLSSKRLMLFLAYSGLMLM
                     LTAPIYYMFRNVIILAFTPALFSLFLSIFNIQFFSIVQRDVDNEFLGRVFGIIFTVAI
                     LFMPLGTVFFSVVLNPNNTFNFFIIGVSITILSLIFSTLLKRYDRS"
BASE COUNT          406 a          182 c          157 g          333 t
ORIGIN      
        1 atcttgattg tattcttcta aaaaggattg cccttcacat tcaactgtga ttgattaaat
       61 tccaaaaata tattccaaag gcgctctaaa ttctctatat cagctatttt ttatcatcta
      121 tcaacttcta tcatacctct tcaatagcgt gctaaatatt agcgataata tcgtaataga
      181 tacaccaata ataaaaaaat taaaagtatt gttaggattt aaaactactg agaaaaaaac
      241 agtacctagt ggcataaaaa gaatagctac cgtaaagatg attccaaata ctctgccaag
      301 aaactcatta tctacgtctc tttgaacaat agagaaaaat tggatattaa aaatggaaag
      361 aaatagacta aataatgctg gagtaaaggc taaaataatg acgttgcgga acatataata
      421 gatcggcgct gttagcatta acatcaaacc tgaatatgct aaaaagagca ttaaacgttt
      481 gcttgacaag gatttattga taaatccact taatatcgca ccaataaagc caccaatcgc
      541 ttcggctgtt aaaaaggttc catatagccc acttgaaata cttccaaaca tttgattact
      601 gtatggcagc aacaaattat aggctgctag gaaaaagtta acaagtgctg aaagggcaat
      661 tatagtgaga atttgtttat gactatatac atacgtaaat cctattttta aatcatcaaa
      721 gattcctctt atcgtcatat tctcttttgt atccacctcc tcatttacag gagtaatgaa
      781 gaaaattagt gaggcagcaa ttaaaaacga taatccatct agtaataaca caccatgtat
      841 tccaagtaac ttatataaga aaattgcaat cataggaatt gttactttaa tcacagtact
      901 tgttgtttct agcaatgaat taagttatga tatactgtcc ttttttacaa tttctttcgt
      961 aaaagctttg tatgatggcc ctgaaaaagc actcataaaa gccaaaatga catttgttat
     1021 gactatcgca taaaccaacc atttttcttg ggatataaat gaaagagtta tgcaagca
//
LOCUS       DAVKGC010000195         1062 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVKGC010000195 DAVKGC010000000
VERSION     DAVKGC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.195"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..>1062
                     /gene="pcpA"
                     /locus_tag="VW049_002055"
     CDS             <1..>1062
                     /gene="pcpA"
                     /locus_tag="VW049_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU9433098.1"
                     /translation="KTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQ
                     NNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQ
                     AFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFD
                     FSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLP
                     KSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKT
                     PKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAF
                     YGNLELKELILPDNVKNFG"
BASE COUNT          408 a          152 c          175 g          327 t
ORIGIN      
        1 aaaaaactac aatattatca ttaactacag ctgcggttat tttagcagca tatgtcccta
       61 atgaaccaat cctagcagat actcctagtt cggaagtaat caaagagact aaagttggaa
      121 gtattattca acaaaataat atcaaatata aggttctaac tgtagaaggt aacataggaa
      181 ctgttcaagt gggtaatgga gttactcctg tagagtttga agctggtcaa gatggaaaac
      241 cattcacgat tcctacaaaa atcacagtag gtgataaagt atttaccgtt actgaagtag
      301 ctagtcaagc ttttagttat tatccagatg aaacaggtag aattgtctac tatcctagct
      361 ctattactat cccatcaagc ataaaaaaaa tacaaaaaaa aggcttccat ggaagtaaag
      421 ctaaaactat tatttttgac aaaggcagtc agctggagaa aattgaagat agagcttttg
      481 atttttctga attagaagag attgaattgc ctgcatctct agaatatatt ggaacaagtg
      541 cattttcttt tagtcaaaaa ttgaaaaagc taaccttttc ctcaagttca aaattagaat
      601 taatatcaca tgaggctttt gctaatttat caaatttaga gaaactaaca ttaccaaaat
      661 cggttaaaac attaggaagt aatctattta gactcactac tagcttaaaa catgttgatg
      721 ttgaagaagg aaatgaatcg tttgcctcag ttgatggtgt tttgttttca aaagataaaa
      781 ctcaattaat ttattatcca agtcaaaaaa atgacgaaag ttataaaacg cctaaggaga
      841 caaaagaact tgcatcatat tcgtttaata aaaattctta cttgaaaaaa ctcgaattga
      901 atgaaggttt agaaaaaatc ggtacttttg catttgcgga tgcgattaaa cttgaagaaa
      961 ttagcttacc aaatagttta gaaactattg aacgtttagc cttttacggt aatttagaat
     1021 taaaagaact tatattacca gataatgtta aaaattttgg ta
//
LOCUS       DAVKGC010000196         1023 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVKGC010000196 DAVKGC010000000
VERSION     DAVKGC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.196"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..720
                     /locus_tag="VW049_002056"
     CDS             <1..720
                     /locus_tag="VW049_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9433099.1"
                     /translation="KYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTIKKA
                     LDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRIIHF
                     KLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDILQKS
                     IYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTPFEY
                     SISRHRYDLFEFNSFALRHSS"
BASE COUNT          333 a          162 c          185 g          343 t
ORIGIN      
        1 aaatatttat ttatttacaa tgagattcgt gaaaagattc tttgtaataa atataccatg
       61 aacgaacaat tgcctgatga aatgacatta gctaaacagt ttgcctgtag tcgaatgacg
      121 atcaaaaaag ctttagactt gttagtttct gagggcttaa tttttagaaa acgtgggcag
      181 ggaacctttg ttctctctcg tggcagctca aaaagaaaat taatcgttcc agaaagagat
      241 atccggggat tgacaaaaat atctgaagat gctcattcta caattgactc gaggattatt
      301 cacttcaaat tagaatttgc aaatgaattt ttagcagaaa aactacaggt cgctttgcag
      361 agtccagttt ataatattta ccgcctgcgt attattgacg gtaaacctta tgttctggaa
      421 caaacttata tgagtaccga tgttattcca ggtattactg aagatatttt acaaaaatcg
      481 atttacaatt acattgaagg aaagttagga ttgcatattg ccagtgctac aaaaatcttg
      541 cgagcttctt ctagttcaga aaatgagcaa cattacttgc agctccttcc aacggaaccg
      601 gtatttgaag tagaacaagt ggcttatttg gataacggaa ctccgtttga gtactcgatt
      661 agtcgtcatc gctatgattt atttgaattt aattcttttg cattacgaca ttcctcctag
      721 gagaaaatgt gaaaatgaag ccaatctttt acagactcta gtttaagaaa aatttaaaac
      781 agggcaagaa ggtcccatct atgcttaaat ggtttctctt ttctaaataa gatggcttta
      841 aaagagtgat cgttgtatcc atcatgttga aaaatatctt cgtatagctt atagagtagg
      901 tactgaaatt gttcacctga tctacttctt atagttattt agttttaaat agtgtttcaa
      961 acattcttac actgacgaga agtttttgag tcttttcttg taacacatat agtatactgt
     1021 ggt
//
LOCUS       DAVKGC010000197          992 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVKGC010000197 DAVKGC010000000
VERSION     DAVKGC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.197"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(231..911)
                     /locus_tag="VW049_002057"
                     /pseudo
     CDS             complement(231..911)
                     /locus_tag="VW049_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          237 a          317 c          234 g          204 t
ORIGIN      
        1 tcggcttcag caagtaccag tgcttcagct tcagcaagta ccagtgcgtc ggcttcagca
       61 tcaacgagtg cttcagtctc agcgtcaacc agtgcctctg aatccgcatc aacaagtgcc
      121 tcggcttcag caagcaccag tgcgtcggct tcggcatcaa caagtgcctc agcctcagca
      181 agtaccagtg cttcagcttc cgcatcaacg agtgcgtccg cttcagcaag cacaagtgcg
      241 tcagcctcag caagtaccag tgcctctgaa tccgcatcaa caagtgcctc ggcttcagca
      301 agtaccagtg catcggcttc ggcatcaaca agtgcctcag cctcagcaag tacaagtgcg
      361 tcagcttccg catcaacgag tgcgtccgct tcagcaagta ctagtgcatc agcttcagca
      421 agtactagca cctcagcctc agcgtcaaca agtgcttcgg cttcagcgtc aacgagtgcg
      481 tctgagtcag catcaacgag tgcgtcagcc tcagcaagca catcagcttc tgaatctgca
      541 tcaaccagtg cgtcagcttc agcgtcaacc agtgcgtcgg cttcagcgtc gacaagtgct
      601 tcggcttcag catcaacgag tgcgtcggcc tcagcaagcg caagtacctc agcgtcagct
      661 tccgcctcaa ccagtgcgtc ggcttcagca agcacaagtg cgtcagcctc agcaagtatc
      721 tcagcgtctg aatcggcatc aacgagtgcg tccgcttcag caagtactag cgcctcagca
      781 tcagcgtcaa caagtgcttc ggcttcagcg tcaacgagtg cgtctgagtc agcatcaacg
      841 agtacgtcag cctcagcaag cacatcagct tctgaatctg catcaaccag tgcgtcagcc
      901 tcagcaagta tctcagcgtc tgaatctgca tcaacgagtg cgtccgcttc agcaagtact
      961 agcgcctcag catcagcgtc aacaagtgca tc
//
LOCUS       DAVKGC010000198          988 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVKGC010000198 DAVKGC010000000
VERSION     DAVKGC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.198"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            105..584
                     /locus_tag="VW049_002058"
     CDS             105..584
                     /locus_tag="VW049_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9433100.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLADDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            678..749
                     /locus_tag="VW049_002059"
     tRNA            678..749
                     /locus_tag="VW049_002059"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:711..713,aa:Glu,seq:ttc)
BASE COUNT          371 a          141 c          229 g          247 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtgtataagt gtagaaatga atattacctt catttatggg
      181 aattgtcgga ttgggaccaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggc agacgatatt ccacgtttaa ggaaatattt caaaaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aagcaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ctagtaagtc atgcaaaaaa
      601 aatgaaaaaa attagaaaaa gtagttgaca aagtttgaaa agactgtata atagtaagag
      661 ttgaaaataa caactcaggt ccgttggtca aggggttaag acaccgcctt ttcacggcgg
      721 taacacgggt tcgaatcccg tacggactat ggtatgttgc gtcaggacca cttgatgaaa
      781 aaaagtttaa aaaagcttaa aaatcttcaa aaaagtgttg acaagcgaaa gcggctgtga
      841 tatactaata tagttgtcgc ttgagagaag caagtgacaa agacctttga aaactgaaca
      901 agacgaacca atgtgcaggg cgctacaacg taagttgtag tactgaacaa tgaaaaaaac
      961 aataaatctg tcagtgacag aaatgagt
//
LOCUS       DAVKGC010000199          974 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVKGC010000199 DAVKGC010000000
VERSION     DAVKGC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.199"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            29..844
                     /locus_tag="VW049_002060"
     CDS             29..844
                     /locus_tag="VW049_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9433101.1"
                     /translation="MSQRELAEGICKQGQISRLESGEFTPGADFLHALAKKLKVSMDY
                     FFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENVKAHRLSLADKIYMEWIK
                     SLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFNFYYDIGDLGRFNKIREK
                     LEYRVNQLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAITKITATIKQCKAYRTNYL
                     LADLYLLMGNVSENFSSKSSVKEYFETAHFLYKLEENMSMALKVEHYIADITE"
BASE COUNT          370 a          128 c          181 g          295 t
ORIGIN      
        1 attaaaaaat agacgaaaag aattaaaaat gtctcagcga gaactagctg aaggtatctg
       61 taaacagggg caaatcagtc gattagagag cggagagttt acccctggag cggatttcct
      121 gcatgccctt gctaagaagt taaaagtcag tatggattat ttttttgatg agcaggttgt
      181 agagaaagtt gaggagttat cggaatttaa gaaattagca cagacattta ttacaaatcg
      241 caattatgag tctttgaaat atatatatga attggagaat gtaaaagctc accgcctatc
      301 tttagcagat aaaatctaca tggaatggat aaaatctctg atagattttt atttttacgg
      361 acagaaagaa gatgcgattg tacgattaga gggggtatta tcacagttaa gtgtatctga
      421 tttgaactat ctccaagttt caaacaccct attcaatttt tactatgata ttggagattt
      481 aggtcgtttc aataaaattc gtgaaaaact agagtatcga gtaaatcaac tcaatttgag
      541 tacaatcgaa gagttagagc tatctattaa gtttaactac aacgtttgtc gttatctatg
      601 gttacagaaa aatattgaag aagctattac taaaattaca gctactataa aacaatgtaa
      661 ggcatacaga acgaattatc ttttggctga tttgtactta ttgatgggaa acgtaagtga
      721 aaatttctct tctaaaagtt cagtgaaaga atattttgag acagctcatt tcctttacaa
      781 attagaggaa aacatgtcaa tggctttgaa agttgagcat tacattgcag atataacaga
      841 ataatgaata cggtgttcaa gtgagcatcg tttttcaaac ccagtttaga tacaaaaaag
      901 tatcaaacaa aaaaaaataa aaaaacatta taaaaatact tgacaagcta attttgaagt
      961 gataaaataa atgc
//
LOCUS       DAVKGC010000200          917 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVKGC010000200 DAVKGC010000000
VERSION     DAVKGC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.200"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..891)
                     /locus_tag="VW049_002061"
     CDS             complement(<1..891)
                     /locus_tag="VW049_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9433102.1"
                     /translation="MVAGQLLLIYAATINALVLNELIAMNLERFLKLSIYQMIVWCGI
                     IFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLN
                     DQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKIFASKMREVSL
                     NLTSQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAG
                     AISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGELGGQLSSIIG
                     TKPIFLKLYSINPIESNKMND"
BASE COUNT          330 a          162 c          123 g          302 t
ORIGIN      
        1 atcattcatt ttatttgact caattggatt aattgaatac aattttaaaa aaataggctt
       61 cgtaccaata atagaggata attgacctcc taattcacct agcgctgtaa aaataacacc
      121 tgttagtgct cctattgctt caatagtacc aattttcact attcctttta ttgcaagata
      181 gcctgttaaa aaaacgagag atatctgaaa aaaaatattg agaaagaagc taatagcgcc
      241 tgctaacgtt tctacagttg tctttctttg tataaccatc tttaataaaa ttcctgcttc
      301 tttaattttc ttaggcaata catataaaag attcaaggac gctaacacat caaatccatt
      361 caatatagtc tcactagatt ttaaaaaagc ttcattttgg ctagttaaat ttagactaac
      421 ttctcgcatt ttcgatgcaa agatttttgg tacaagtagc ataatcatta atgaaaacaa
      481 ggtggctaca gtcaatgacc aatgatagtg attaagagtc acaactgcaa atatagtacc
      541 agaaattcct tttattacta aaaaaagttg tttaaaagcc tgatcattta aagtctgaac
      601 atcattattt agccacgaaa gatatgttcc tgatgattta ctatgaaatt cttgataggt
      661 agagttagag atgtctgtgg caactctatt tcgaatctct agattaaact cttggatcac
      721 ttcaacctga taatttttca ctacccagtc aaggaatatt atcccacacc agacaatcat
      781 ttggtagatt gacaatttca aaaaccgctc taaattcatc gcaattaatt cattcaacac
      841 cagagcatta atagttgctg cataaattag caataattga ccagcaacaa taaatatcgt
      901 taataaacta aattttt
//
LOCUS       DAVKGC010000201          826 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVKGC010000201 DAVKGC010000000
VERSION     DAVKGC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.201"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(245..454)
                     /locus_tag="VW049_002062"
     CDS             complement(245..454)
                     /locus_tag="VW049_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433103.1"
                     /translation="MFFAGIGEIHFLLFLLYLSMSASPIIGLVIGYTKINLINSIIIA
                     LGMILALILVLSRVYRKYFYRDDDD"
BASE COUNT          322 a          160 c           93 g          251 t
ORIGIN      
        1 tccccttgga ctcaatcaat ggaaaataaa atattgcaac tagaagcgaa taaagtcccg
       61 ctaataaata tttctgatca tctttcattt aaaacaccat attatttact aagaatacat
      121 caatacaata gagcaactct gtaagtcaat atttgataaa gcaaagcttt aaacccttcc
      181 ccagacgtta gcttttattc aacccactac agttgacaag gagcccaaat tattgataaa
      241 aactttagtc atcatcgtct ctataaaaat attttctata cactcttgat aataccaaaa
      301 ttaatgccaa tatcatacca agagctataa taatagaatt tattagattt attttagtat
      361 atccaatcac tagtccaata attggactag cagacatgct tagatataat aaaaataaca
      421 aaaaatgaat ttcaccaatt cctgcaaaaa aaatgtttca tagctatagt tttacgccca
      481 taaataccca tcaaatttac cgtctacgat atctaaacca tatgcaatgg ctcctcctaa
      541 tgagaagact tcagcaacag tagccgcaat attaagagca ttagtaatag ttgccatact
      601 aactccacca atatatcttg ttatagcact tagttaccta gataagatat cctttagctt
      661 gcttccaaaa tttaagtctg cacctataat atgagttcat attagtactg ctaaatccta
      721 tctatagtaa tgtcaaacac ctaaataaag tcatctctcc aattcattca agaaatgaag
      781 aaaggcaaaa aatctttaaa ataagaaaaa tgcatattat caggtg
//
LOCUS       DAVKGC010000202          804 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVKGC010000202 DAVKGC010000000
VERSION     DAVKGC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.202"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            3..800
                     /locus_tag="VW049_002063"
     CDS             3..800
                     /locus_tag="VW049_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9433104.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
BASE COUNT          199 a          143 c          182 g          280 t
ORIGIN      
        1 ttatgatttc ttcagaattt atctcaaaga ttgaatttgc ttgcaataag aaagaaagtc
       61 tttatagtca aagcaaattt aagtatgcga ttcgttcgat gttcgcaggt gcatttttaa
      121 ccttcagtac tgctgcaggt gcagttgggg ctgacttgat taataaaatt gcaccaggta
      181 gtggacgctt cctctttcca ttcgtttttg cttggggctt ggcctacatt gtttttttga
      241 atgccgagtt ggtcacttca aacatgatgt tcttgactgc tggtagtttc ttaaaaaaaa
      301 tctcttggag aaaaacagct gagattttac tatactgtac cttgttcaac cttatcggag
      361 ccttgatagc agggtggggc tttgctcatt cggcagccta tgcgaatctg acacacgata
      421 gtttcatctc aggtgttgtt gagatgaagt taggccgctc aaatgaattg gtcttgcttg
      481 aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct gtcatttatt ttggtcaaag
      541 atggtggtgc caaactttgg cttgtgttgt cagctattta catgtttgta ttcttaacaa
      601 acgagcacat tgcggcgaac tttgcttctt tcgcgattgt gaaattcagt gttgctgcgg
      661 attcaattgc caacttcggt gttggaaata tgcttcgcca ctggggtgtg actttcatcg
      721 gaaactttat cggaggaggc ctcttgatgg gtcttccata tgccttcctc aataaaaacg
      781 aagatactta tgtagattaa gaaa
//
LOCUS       DAVKGC010000203          782 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVKGC010000203 DAVKGC010000000
VERSION     DAVKGC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.203"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            359..730
                     /locus_tag="VW049_002064"
     CDS             359..730
                     /locus_tag="VW049_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEU9433105.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
BASE COUNT          244 a           94 c          176 g          268 t
ORIGIN      
        1 gagcttgagt tgttagtgga ggataaaatg ttaagagtaa tagtagttat tcaatcttta
       61 gtaataatac tgcttgctgt tgctttatta ttatctcgga aaaactttga ttcgttaaat
      121 gttgttctta taatgatgat cgtatcacaa ttgttgttat tagtatattt gataaaatca
      181 gggaatcagt gattagctgt tatttgaatt aagtaaactg ttaagcttca agcagtaact
      241 ggttttaaat atttaaaata agataaagga aggaagtcta atttaggtaa ctaaacaaag
      301 cctgcgatca cttgttagga aaccatccta agcatgctta ataaaaagga gaaaacaaat
      361 ggaacttgta ttaccaaata attatgttgt gattgatgaa gaagagatga tgtaccttga
      421 tgggggggct atatatatac ccaggtgggc aattacagga gccattactg gtgcagcata
      481 tgcagcatta gcagcagcag gaggtggagg ccttcaacta gttcttgcat cttatggatt
      541 acgctccgca ctggtagctg ggattgttaa aggtttagga gtattaggaa ttcatattgg
      601 aaatgctttt gcaaatactg ttattagaag tattgcatct gctggaattg gtgctggagc
      661 tgattggatt tttaccaata ttattgatgg ctgggatggg cgacgtgata atcaattgag
      721 aataggttaa taatcttatg ttgattgttt taatttcagt tattgttgca ttcctgtatc
      781 at
//
LOCUS       DAVKGC010000204          772 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVKGC010000204 DAVKGC010000000
VERSION     DAVKGC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.204"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(71..730)
                     /locus_tag="VW049_002065"
                     /pseudo
     CDS             complement(71..730)
                     /locus_tag="VW049_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chordopoxvirus G3 family protein"
BASE COUNT          187 a          246 c          183 g          156 t
ORIGIN      
        1 cagcatcaac gagtgcatcg gcttcagcgt caaccagtgc atcagagtca gcaagtacca
       61 gtgcgtcagc ttccgcatca acaagtgcat cggcttcagc aagtactagc gcctcagcct
      121 cagcgtcaac aagtgcttca gcttcagcgt caaccagcgc ctcggcctca gcaagtatct
      181 cagcgtctga atcggcatca acaagtgcct cggcttcagc gtcaaccagt gcgtcggctt
      241 cagcaagcac cagcgcctca gtctcagcat caacaagtgc ttcagcctca gcatcgacaa
      301 gtgcctcggc ttcagcaagc acatcagcat ctgaatcagc gtcaaccagt gcgtcggctt
      361 cagcaagtac cagtgcttca gcttcagcaa gtaccagtgc gtcggcttca gcatcaacga
      421 gtgcttcagt ctcagcgtca accagtgcgt cggcttcagc aagtactagt gcatcggctt
      481 cagcatcgac aagtgcgtct gaatctgcat caacgagtgc ttcggcttca gcgtcaacga
      541 gtgcgtctga gtcagcatca acgagtgcgt cagcctcagc aagcacatca gcttctgaat
      601 ctgcatcaac cagtgcgtca gcctcagcaa gtactagtgc gtcagcctca gcaagtacca
      661 gtgcgtcagc ctcagcaagt accagtgcct ctgaatccgc atcaacaagt gcatcggctt
      721 cagcaagcac cagtgcgtcg gcttcagcaa gtactagcgc ctcagcctca gc
//
LOCUS       DAVKGC010000205          750 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVKGC010000205 DAVKGC010000000
VERSION     DAVKGC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.205"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            150..>750
                     /locus_tag="VW049_002066"
     CDS             150..>750
                     /locus_tag="VW049_002066"
                     /inference="COORDINATES: protein motif:HMM:NF016357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEU9433106.1"
                     /translation="MKLIKLLKKYINLYISSSVPKTNKLLIFGSWLGEKYADNPRYLF
                     EYVIKNRPDLKAIWITSNQDVFKELQKKQYLVMMAEAPETRKIVKKAKYIFTATGIFD
                     IGEQNANFVGGAYLINLWHGIPLKKIMYDDKHSALHKRSKLVTWVEKIPLRNYFVIST
                     STAITQIYQSAFRVKKSNILELGQPRNDYFYDKSNPVSSL"
BASE COUNT          269 a           99 c          125 g          257 t
ORIGIN      
        1 aaaatatttg cttagcggtg ctatatgctg cttagttctt ataacttatc tatttcttcc
       61 aaatgtagaa aattttgaaa tcatattttt tatttacctt ttcattggga tggatgaact
      121 agcactatat acaaaaggaa tgaatgtaca tgaaattgat taaattattg aaaaaatata
      181 taaatttata cattagtagt tcggtgccta agacaaataa attgttaatt tttggatcat
      241 ggttaggtga aaaatatgca gacaatccaa gatatttgtt tgagtatgtg attaagaatc
      301 gtcccgattt aaaagctata tggattacaa gtaatcaaga tgtttttaaa gaattacaga
      361 agaaacaata tctcgtgatg atggcagagg cacctgaaac tcggaaaatt gttaaaaaag
      421 caaagtatat ttttacagca actggtattt ttgatattgg agaacaaaat gctaacttcg
      481 ttggaggtgc ctatctaatt aatctatggc atggcattcc tttgaaaaaa atcatgtatg
      541 atgataaaca ttcagcactt cacaaacgta gtaaactagt gacttgggta gaaaaaattc
      601 ctcttcgaaa ctattttgtt atttctacta gtacggcaat aactcaaatt tatcagtctg
      661 cttttcgcgt aaagaaatct aatatattag aacttggtca acctaggaat gactattttt
      721 atgataagtc taatccagtg agttcattaa
//
LOCUS       DAVKGC010000206          736 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVKGC010000206 DAVKGC010000000
VERSION     DAVKGC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.206"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            1..736
                     /locus_tag="VW049_002067"
                     /pseudo
     CDS             1..736
                     /locus_tag="VW049_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          222 a          157 c          122 g          235 t
ORIGIN      
        1 cgtcgtttca agtgctatca ttgttctaaa atgatggttg ctgaaacttc tatcgtcaag
       61 aagaatcacc aaatccctcg tattatcaac caaaaaattg cgcaaaagtt aattgaaaag
      121 atttctatga ccgatattgc ccatcagctt tccatctcaa cttcaactgt tattcgtaag
      181 ctcaatgatt ttcactttaa acatgatttt tcttgtcttc ctgagattat gtcttgggat
      241 gagtatgctt ttacaaaagg aaagatgagc ttcattgcgc aagattttaa caatctcaat
      301 atcatcactg ttcttaaggg aagaacacaa gctgtcatcc gaaatcactt tcttaaatat
      361 gatagagccg tccgatgtcg cgtcaaaatc attactatgg atatgtttag tccttactat
      421 gacttagcta gacagcttcg ctttcaaatt tctaggctca ggctgaaaca gtctcccaga
      481 ctgtttcact cccgaatgct aaaatcgttc tagatcgttt ccatattatc caacatctca
      541 gccgtgctat gagtcgtgtg cgtgtccaaa tcatgaatca gtttcatcga aaatcccatg
      601 aatacaaggc tatcaagcgc tactggaaac tcattcaaca ggatagccgt aaactgagtg
      661 ataagcgatt ttatcgtcct acttttcgca tgcatttaac caataaagag attttagaca
      721 agcttttgag ctattc
//
LOCUS       DAVKGC010000207          735 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVKGC010000207 DAVKGC010000000
VERSION     DAVKGC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.207"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            26..295
                     /gene="blpJ"
                     /locus_tag="VW049_002068"
     CDS             26..295
                     /gene="blpJ"
                     /locus_tag="VW049_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEU9433107.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            364..612
                     /gene="blpK"
                     /locus_tag="VW049_002069"
     CDS             364..612
                     /gene="blpK"
                     /locus_tag="VW049_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEU9433108.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
BASE COUNT          222 a           93 c          189 g          231 t
ORIGIN      
        1 tgaaaataaa aaggaggtat tcatcatgaa tacaaaaatg ttgtcacagt tagaggttat
       61 ggatactgaa atgcttgcga aagttgaagg ggggtatagc tccactgatt gtcaaaatgc
      121 actgattaca ggagtcacta cgggaattat aactggtgga actggagcag gtttggccac
      181 tttaggagta gctggacttg ctggtgcatt tgttggagca cacattggtg caattggggg
      241 tggattaaca tgcttaggag gtatggttgg tgataaattg ggactaagtt ggtaagactt
      301 atagtaaaat tatgaggatt ctattctgat ttattttagt tttttaaaaa aggagtattt
      361 gtcatggata caaaaatgat gtcacaattt tctgttatgg atactgaaat gcttgcttgc
      421 gttgaaggtg gcggatgcaa ttggggagat tttgccaaag caggtgttgg aggagcagct
      481 gtagttgctg ctttaggttg tgcagcgggt ggagtgaaat atgggaaaat tctaggacca
      541 tggggcgctg caataggagg aattggagga gcagtggttt gtggatattt agcctatacc
      601 gctacatcat aaaaatgaaa gctaaatttt atgaaggcta tattctttat tattctcttc
      661 gcttttcaaa cctatcttat ttatctatcc ataagtatct ctgataaaaa acaaaagact
      721 attgaattaa catca
//
LOCUS       DAVKGC010000208          707 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVKGC010000208 DAVKGC010000000
VERSION     DAVKGC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.208"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            1..707
                     /locus_tag="VW049_002070"
                     /pseudo
     CDS             1..707
                     /locus_tag="VW049_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          205 a          153 c          135 g          214 t
ORIGIN      
        1 ctagaattct ccttagaaag cgtcgtttca agtgctatca ttgttctaaa atgatggtcg
       61 ctgaaacttc tatcgtcaag aagaatcatc aaattcctcg tattatcaac caaaaaattg
      121 cgcaaaagtt gattgagaag atttctatga ccgatattgc ccatcagctg gccatttcaa
      181 cttcaactgt cattcgaaag ctcaatgatt ctcactttga gcatgatttt tcgcgtcttc
      241 ctgagattat gtcctgggac gttgaaacag tccggggagt gactgtttca atcgggagat
      301 ggagatgagc tttattgcgc aagattttaa caatctcaat atcatcactg ttcttgaagg
      361 tagaacacaa gctgtcatcc gagatcactt tcttaaatat gatagagccg tccgatgtcg
      421 cgtcaaaatc attactatgg atatgtttag cccttactat gacttagcta aacagcttcg
      481 ctttcaaatt tctaggctca ggctgaaaca gtctcccagg ctgtttcact cccgaatgct
      541 aaaatcgttc ttgatcgctt tcacattgta caacatctta gccgtgctat gagtcgtgtg
      601 catgtccaaa tcatgaatca gtttcatcga aaatcccatg aatacaaggc tatcaagcgt
      661 tactggaaac tcattcaaca ggatagccgt aaactgagtg ataagcg
//
LOCUS       DAVKGC010000209          704 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVKGC010000209 DAVKGC010000000
VERSION     DAVKGC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.209"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            291..590
                     /locus_tag="VW049_002071"
     CDS             291..590
                     /locus_tag="VW049_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9433109.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          261 a          130 c          120 g          193 t
ORIGIN      
        1 tagatagatg taacatgttt gcaaacaaag catacgaacc tttagtaaaa tcatttccat
       61 gaaactagaa tagagccctc ttagcaaaaa tcattatttt aatttatttc taatcactcc
      121 ttgacataaa taactctcac caataaaaga ctatgtctta aaaaaatggt ataataaaat
      181 caatacttgg gcttgatggc tatgctacta ataacaatta ggagagaaaa tcaggcactt
      241 gttaacaaca aggattatcc ccttgagatg aaaggaactt tagaaatctt atgatgaaca
      301 tgcaaaacat gatgcgtcaa gcacaaaaac ttcaaaaaca aatggaacaa agccaagctg
      361 aacttgctgc tatgcaattt gttggcaaat ctgctcaaga tcttgtccaa gcgaccttaa
      421 ctggcgataa gaaagttgtc agcattgatt tcaatccagc tgtcgttgac ccagaggacc
      481 ttgagactct ttctgatatg accgttcaag ccatcaactc tgctcttgaa caaatcgatg
      541 aaactaccaa gaaaaaactg ggtgctttcg ctgggaaatt acctttctaa aaacaaggag
      601 ctagaacaat gcttgtcgat aacaaaggct aagaaaggtg caaaaatgac tctataatat
      661 ttgtagtggg taaatccact atagatatta tggagcctat ttta
//
LOCUS       DAVKGC010000210          696 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVKGC010000210 DAVKGC010000000
VERSION     DAVKGC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.210"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..>696)
                     /locus_tag="VW049_002072"
     rRNA            complement(<1..>696)
                     /locus_tag="VW049_002072"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          158 a          220 c          142 g          176 t
ORIGIN      
        1 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
       61 gcaccacctg tcacctctgt cccgaaggaa aactctatct ctagagcggt cagagggatg
      121 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      181 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      241 cttaatgcgt tagctacggc actaaacccc ggaaagggtc taacacctag cactcatcgt
      301 ttacagcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      361 cgtcagttac aagccagaga gccgctttcg ccaccggtgt tcctccatat atctacgcat
      421 ttcaccgcta cacatggaat tccactctcc cctcttgcac tcaagttaaa cagtttccaa
      481 agcctactat ggttaagcca cagcctttaa cttcagactt atctaaccgc ctgcgctcgc
      541 tttacgccca ataaatccgg acaacgctcg ggacctacgt attaccgcgg ctgctggcac
      601 gtagttagcc gtccctttct ggtaagatac cgtcacagtg tgaactttcc actctcacac
      661 tcgttcttct cttacaacag agctttacga tccgaa
//
LOCUS       DAVKGC010000211          690 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVKGC010000211 DAVKGC010000000
VERSION     DAVKGC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.211"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..>690
                     /locus_tag="VW049_002073"
     CDS             <1..>690
                     /locus_tag="VW049_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433110.1"
                     /translation="SKSISETEFEYLDGELDLIREQLDEYFNNNEYSDEQLNFLSKTI
                     YKAMLVGFDRNSVTGLVIAGYGVEEIFPSLRQIELSGVFAKRLVWRVITEKEISQNVA
                     CHIIPFAQSEMVDTIMNGIDPMLNAFIGQQINEILESNNISDAQEELFETIARVQQQY
                     YINPIFELIGMQPIDEMASTAKTFIELTSFKRKIVNTLETVGGPVDVLAISKGEGPIW
                     IERKHYFNIEN"
BASE COUNT          243 a           95 c          133 g          219 t
ORIGIN      
        1 tctccaaatc aatatctgaa actgaatttg aatatttaga tggcgaactt gatttaatac
       61 gggaacaact agatgagtac tttaacaata atgaatactc cgatgagcaa ctaaactttt
      121 tatctaaaac aatatacaaa gcaatgttag ttggttttga tagaaattct gtaacagggt
      181 tggtaattgc tgggtatggt gttgaagaaa tttttccatc ccttagacaa atagagttaa
      241 gtggtgtatt tgctaaacgc cttgtctggc gtgtaattac cgagaaagaa attagccaaa
      301 atgttgcttg tcatattatt ccctttgctc aatccgaaat ggtagatact ataatgaatg
      361 gcatagatcc aatgttaaac gcatttatag gacaacaaat taatgaaatt ttggaaagta
      421 ataatatatc ggatgcacag gaagagttgt ttgaaactat cgctagagtc caacaacaat
      481 attatataaa tccaattttt gagttgattg ggatgcaacc tattgatgag atggcttcta
      541 ctgcaaaaac attcattgag ttgacttctt ttaagagaaa gattgtgaat acacttgaaa
      601 cagttggagg accagtggat gttttagcta tttctaaagg ggaaggtcct atctggattg
      661 aaagaaaaca ctactttaat attgaaaata
//
LOCUS       DAVKGC010000212          672 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVKGC010000212 DAVKGC010000000
VERSION     DAVKGC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.212"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..225
                     /locus_tag="VW049_002074"
     CDS             <1..225
                     /locus_tag="VW049_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9433111.1"
                     /translation="PNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFGENKYRLLNRK
                     YEMDGINALHPEDLIDYQKVFLNHHINCYF"
BASE COUNT          238 a          125 c           88 g          221 t
ORIGIN      
        1 cctaatttta acaatagttt agaggatgca gaaaaattcg ctactctatt taactcatta
       61 aatatcgacc aagttcaact actccctttt catcaatttg gtgaaaacaa atatcgttta
      121 ttaaatcgga aatatgaaat ggatggaatc aacgcacttc atcctgaaga tcttattgat
      181 tatcaaaagg tatttctgaa ccaccatatt aattgttatt tctagtttat ttccttgaaa
      241 tgctctagct atttgcagat aacaagcatc tataatacat acttaacttt tcaaaaggtt
      301 tagctaaaaa attttagcca aaccttttct attttacctt gctctagaat ttttaaactg
      361 ctatacttat cacaaaaaaa ccttccaagg aggtagcgga tgtctcattc ctttaaaaaa
      421 tctctccaaa aagaaattct ccatagaagc tctatcgcag cctttgtgac ctcacgcgca
      481 ttctccgata cagtatcacc gatttagaaa aagaaacaac catttgtcag gatttaaaaa
      541 gtatctccat atttccccaa aggaattgaa atcactcaga agaatagtaa aataggctat
      601 tcttcttttt gtagagaatt ttactctaat atagtgaatt gaaataagat gtgaacatct
      661 ctatcaggaa ag
//
LOCUS       DAVKGC010000213          669 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVKGC010000213 DAVKGC010000000
VERSION     DAVKGC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.213"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            326..>669
                     /locus_tag="VW049_002075"
     CDS             326..>669
                     /locus_tag="VW049_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEU9433112.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHISKEFNDRSILWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLT"
BASE COUNT          292 a          103 c          112 g          162 t
ORIGIN      
        1 gaaataacat aattgtccct tggttacaag ggcgcaatta tacaccctaa agggtacttg
       61 cgtcctgcgg agtcaaaccc ttgcagggag caacaaccat tcgcttttta aaagtcaagg
      121 gtaaataaac tcgctaaggc tcgcccttga cttttaaaat ttgaaatgaa agtaacaatt
      181 aagccgacat actgaaacaa caaaaattaa ttcagtagtc ggctttttta attctatcat
      241 ttcaaaacgc tcattcacaa agaggatata aaaaaactat taagcctcca cctaaaacaa
      301 caaagaaaga aaggaagtga taaaaatggc agacagtttt catttttcag taaacataat
      361 atcaagagga aaaggcaaaa gtgcagtagc aagtgcagca tatataagtg gagaaaaaat
      421 aaaaaatgaa tgggacggag taacccatga ctacacaaga aaagaaaaaa tattagtaaa
      481 aaatataata ttgcctgatc atatatcaaa agaatttaat gataggtcta tcttatggaa
      541 taaagtagaa atggcagaaa aaaattctaa tgcacaacta gcaagacaat tcataatagg
      601 actaccaaaa gagttatctt taagtgaaaa taaaaacctg gttgaaagat atataaaaga
      661 aaatttaac
//
LOCUS       DAVKGC010000214          664 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVKGC010000214 DAVKGC010000000
VERSION     DAVKGC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.214"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..587)
                     /locus_tag="VW049_002076"
     CDS             complement(<1..587)
                     /locus_tag="VW049_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU9433113.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQE"
BASE COUNT          229 a          133 c           78 g          224 t
ORIGIN      
        1 tcctcttgat acatttttaa ttctttagtt tttcccatat aactaaccat actactatca
       61 cttacatatg ggaaatcctc ataatatatt actttataac gcataaattc aagcgccctt
      121 ccaatactat tcacaaaaac atgatcaaca tggtcaccaa gtgaaagcgg acaatatacg
      181 acacatttgt cgtctaaatg cattaacagc tcttttatga tatcattctt taatgtgtcc
      241 tcatttttta attcactata gatatgacgg tatagaaaat tgccatttct atctttccta
      301 tagagacatt catagtacga taagtgtcta aaatcacatt gtagacgttc acaagctaac
      361 ctatcttctt tcttcctttc ttcaatcgga tatttcccaa ggttacacaa cttatgaaat
      421 tgcttagcag agggctgtag ctgttgactc aaagggtaac cagaaaatat agtaataaca
      481 agtacaattt ctccttctga agttaatttt gaaatataat caccacagga aaaaattgcg
      541 tcatctaaat gtggagataa aaagatatac ttagtattgt tactcataac cattccctct
      601 acaatttatc taaaaactca ctaagtgtct gattaaattc cacatcatca aaaaaattca
      661 cctt
//
LOCUS       DAVKGC010000215          659 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVKGC010000215 DAVKGC010000000
VERSION     DAVKGC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.215"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            181..432
                     /locus_tag="VW049_002077"
                     /pseudo
     CDS             181..432
                     /locus_tag="VW049_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          209 a          129 c          115 g          206 t
ORIGIN      
        1 ctaaagtcca agtggtacac ttggaataag ccactgtgga ttagttcatt tcctccaatc
       61 gcttgacgat ttctgaaagg ctctaaccac agctttttgt ttcaattggt aatgagtttg
      121 atagcgcata aacgttttat attgtgtaag gtaacaaggc acaaagttcg tggataacca
      181 agtgagcttg ggaatcgttt aggtgacgtt atcttagttg agattcgaag tattcatatc
      241 ttcgataagc ctcatcaatt acaagctttt gctggaccga atccttctat ttaccagtca
      301 ggtcaaatcg atttagcagg gaaaatggtc aaactgggtt cccctcatct acgttgggca
      361 ctcatacaag ctaccaaagc atatgctcgc ttttcgcctg tttttaaagc ctatcttagg
      421 actaagttgt aacaaagaaa gcattacaat gtagccatca ttcaccttgc caaaaaactt
      481 attagaaatc tgttttatct cctaaaaaac aagctagcat ttgacgaaaa aaagtgttct
      541 aactcctgaa tttgccacaa gataaccttt gaaaaaataa actttttcaa ggacattttg
      601 ccgtgctcta aaatcatcca ctttgaaaca agaagtattt cagattaaac tattgaatt
//
LOCUS       DAVKGC010000216          658 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVKGC010000216 DAVKGC010000000
VERSION     DAVKGC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.216"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..>658)
                     /locus_tag="VW049_002078"
     CDS             complement(<1..>658)
                     /locus_tag="VW049_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9433114.1"
                     /translation="IFDMKRSTNEFIGRSAMVTKLLCIYSEGDPFFGVNINSQKEFWN
                     HFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKLGTMLFEYSKLFMENKEDNSTMDSS
                     NNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYT
                     DFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVA
                     E"
BASE COUNT          210 a          127 c           98 g          223 t
ORIGIN      
        1 cttcagcaac atttatatat tctaagtaag aatcccaagc ctcatcaaaa ttatcttgtc
       61 ctccaatcaa ttgggtttct tttgctttct gacaaaaatc tttaaaaata ccgtcctgta
      121 gcctaaactc aatagctcca tccccatttg atactggtct taaaccttct acaaaatccg
      181 tataatcata tgatgggtga aattgtacaa atccgatttg gtcttcgttg ccatccgtta
      241 attctttggc aatttcttta gcaagataag tctttcctgt gccaggagca ccacgaagga
      301 taaggtttgg agactttaat agagattgag ttaattgatg acgaaaattg tttgatgaat
      361 ccattgtact attgtcttcc ttattttcca taaaaagctt agaatactca aaaagcatag
      421 ttcctaattt cgatggctcc aactcaggat aagttttgga caccagttca attattttat
      481 gattttgcag ataaggtcca ccttgatttg tctgagaaac aaagtggttc caaaattctt
      541 tctgactatt aatatttaca ccaaagaaag gatctccctc agaatagata caaagtaatt
      601 ttgtcaccat agcagaacga ccaataaatt catttgttga tcttttcata tcaaaaat
//
LOCUS       DAVKGC010000217          654 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVKGC010000217 DAVKGC010000000
VERSION     DAVKGC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.217"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            1..654
                     /locus_tag="VW049_002079"
                     /pseudo
     CDS             1..654
                     /locus_tag="VW049_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          220 a          124 c          118 g          192 t
ORIGIN      
        1 caagaaaagt tgatagagat agacttaaaa actatcttac tgacaatcca gacgcttatt
       61 tgactgaaat agcttctgaa tttggctgtc atccaactac catccactat gcgctcaaag
      121 ctatgggtta cactcgaaaa aaagaaccac acctactatg aacaagaccc agaaaaagta
      181 gccttatttc ttaagaattt taatagttta aagcacctag cacctgttta gattgacgaa
      241 acaggattcg atacttattt ttatcgagaa tatggtcgct cattaaaagg tcagttaata
      301 agaggcaaag tatctggaag aagatatcag agaatttctt tggttgcagg tctaacaaat
      361 ggtgagttaa tcgctccaat gacttacgaa gagacgatga cgagcgactt ttttgaagct
      421 tggtttcaga agtttctctt accaacatta accacaccat cggttattat tatggataat
      481 gcaagattcc atagaatggg taagttagaa ctcttgtgtg aagagtttgg gtataacttt
      541 ttacctcttc ctccctactc acctgagtac aatcctattg agaaaacatg ggctcatatc
      601 aaaaagcacc tcaaaaaggt attaccaagt tgcaatacct tttatgaggc tctt
//
LOCUS       DAVKGC010000218          643 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVKGC010000218 DAVKGC010000000
VERSION     DAVKGC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.218"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..634
                     /locus_tag="VW049_002080"
     CDS             <1..634
                     /locus_tag="VW049_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9433115.1"
                     /translation="SKLKVPYLKEGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGI
                     IGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEV
                     FNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFVF
                     FDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          253 a           73 c          121 g          196 t
ORIGIN      
        1 aagtaaatta aaagttcctt atttaaaaga aggtggagag tatcagataa aaaatcttag
       61 ctacaaattt actgatgatg agtgcttaag cttaaaagat atttcgttca agcttgggaa
      121 aatttatggc ataataggat ccaacggacg aggaaaatca acgcttttaa gatgtttaat
      181 aggtcttgag aaaaaatcaa aagaagaaat ttattttaag ggagagaagc tatctaaaaa
      241 agaaagactc aaaaactctt cacttgttat gcaagatgta aatcatcaat tattcacaga
      301 tgaagtattc aacgagctta gattaggagt aaagaatttt gatgaagaaa aggcgaaaat
      361 cattttaaaa gatttaggcc tggacgaatt tattgaaagg catccgatga gtttatcagg
      421 agggcaaaag caaaggcttg caatagcatc tgttatgtgc aagaattctc catttgtctt
      481 ttttgacgaa ccttcaagtg gtatggatta ttccaatatg ataaaaatat ctgaactgat
      541 taataagtat aaaaccatgg ataaaataat ttttattgtt tcccatgata tagaattttt
      601 aaatgaagtg gcagatgaaa tttttgaatt ataaaataga gtc
//
LOCUS       DAVKGC010000219          607 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVKGC010000219 DAVKGC010000000
VERSION     DAVKGC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.219"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..425)
                     /locus_tag="VW049_002081"
     CDS             complement(<1..425)
                     /locus_tag="VW049_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU9433116.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYT"
BASE COUNT          207 a           96 c           96 g          208 t
ORIGIN      
        1 gtgtagataa aatccacata ttgaattaaa tcaataaatt tttcatgatc aacaaaggca
       61 gtagtttcaa tggcagtgtg tatgtgatgt tctttagctg attttaagat ggctttagca
      121 aattcaaact gagcaaatat ttcacctcct gataaagtta aacctccacc ggattcttcg
      181 taaaattctt tgtcttttaa tacctctgta ataatttctt ctacagtctt ttcttcaccg
      241 actaaggtga atttctctcg ttgagcatct ttcatttttt caggtttcat tctttgagat
      301 tcaggattag aacaccatgg acagcgcaga ggacatcctt ttaaaaaaac agttgtacga
      361 atacccgggc cgtcatgaat tgaaaagtgt tgaatattaa aaataattcc tttagatatt
      421 tccataagag ttcctcctct tcataagtct atcctatcac aaaaacgaaa gaaaaacaat
      481 tacgaacgaa aggttaattt attgtttttt gctttttatt ttggtagaat aaaacggagg
      541 tgtaaaatga aaagattgga acaaattatt aaattagtat cagaatatga aaagatcgac
      601 gttaata
//
LOCUS       DAVKGC010000220          605 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVKGC010000220 DAVKGC010000000
VERSION     DAVKGC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.220"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
BASE COUNT          184 a           98 c           92 g          231 t
ORIGIN      
        1 cctgttcgct cacaataaga gagaactttt ttacgaaaat ctattgaata tgccataaaa
       61 ggattatacc acattgtgta ctatttttgg ttcattttac tatatcactt tggactatat
      121 cggtctagtt atgtctcaac gattatagct ggatactaag agaaatcaaa aaagcactct
      181 aggatagagg cctaaagtgc ttagtttcaa ggctttacag cctatcatat ttaataaaat
      241 attacaacat tttgttgtat cgctttttta agtattcagc aagtagcttt attacttctt
      301 aatgcttgat aatagggtga tggggagttt tcacttttcg tttgaacgtt ttggagtttc
      361 tcccttactc cacaaatcac tgaacatgat aaacgagttc agcaggcaag aaactagcac
      421 tatttaaaag tgcttttttg tttctattta agtaggaagc tattgcacat cttttaatat
      481 ttgttttatt agtttgcatg aagcattcta aatactcttc atcttctaat ctaaaatcta
      541 acaatagcat aaatttaatt tcgtatctgt tctgttatat tggaactcta tatgtacctg
      601 ttctt
//
LOCUS       DAVKGC010000221          600 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVKGC010000221 DAVKGC010000000
VERSION     DAVKGC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.221"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
BASE COUNT          206 a          118 c           76 g          200 t
ORIGIN      
        1 cgcaatgcct ttggttttcg aaactttgaa aacttcaaaa aacggatttt tatcgctttg
       61 aacatcaaaa aagaaaggac gaaatttgtc ctttctcgag cttagctgac ttcaacccac
      121 tacagttgac aaagagccga aaataattag ttcagaattt tcttacttcc tttttaaatc
      181 cgataatata taggttttgc tagcctctac accataaata tttgctaata aaaatactgt
      241 attattaccc tcttaaggtg cgaaattatt caagttctta aaaatgcttc tctatttccg
      301 taagatagct tatttcttcg atttaagaac tgaagatgat agttctaacc tttagaaatc
      361 caagccttca ctagctcaaa aactcctatc tttcttcctc aataaatcat ctgagagata
      421 ggtattcctt atatccttca aattcatcac tcctttggat attgaggtgc tagcctagaa
      481 gcagacaccc gattatctaa accatcctta ataaagatga caagacagat aaactcagtt
      541 aattacttat atatcctttt aatatggtat atttgtcaac aaaaagaaat caaatcatac
//
LOCUS       DAVKGC010000222          597 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVKGC010000222 DAVKGC010000000
VERSION     DAVKGC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.222"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            186..560
                     /locus_tag="VW049_002082"
     CDS             186..560
                     /locus_tag="VW049_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU9433117.1"
                     /translation="MESIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGGIG
                     TSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQTQLAELEINK"
BASE COUNT          181 a           88 c          129 g          199 t
ORIGIN      
        1 ttactggcta attaatcagt taaacactag ttaaggagta atgatgaaaa aaagaacaat
       61 actattattg atggccagtc tgttagctct tgtcttagga gcatgtggtt tcttggacat
      121 attgttcctg gatcattctc atcaggatta ctatttactg ctattttaga aactggggtg
      181 gtttgatgga aagtattggt cttgttatcg tttcacattc caaacatatt gcagaaggtg
      241 ttgttgaact aattagtaaa gtagctaaag atgttccgat tacttatgta ggaggaaccg
      301 agggcggagg aattggaacg agttttgatc aagtagatag ggttgtttcc gaaaatccag
      361 cagatacttt acttgccttt tttgacctag gttctgctaa aatgaactta aaaatggtga
      421 ctgatttcag tgataaaagt atcatcatca acagggttcc aattgtagaa ggtgcctata
      481 atgcagctgc tcttcttcag gctggtgcag aactgtcagt tattcaaaca cagttagcgg
      541 agcttgaaat caataaataa ggaattttac tataactctt tttataggta agctatt
//
LOCUS       DAVKGC010000223          587 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVKGC010000223 DAVKGC010000000
VERSION     DAVKGC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.223"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..545
                     /locus_tag="VW049_002083"
     CDS             <1..545
                     /locus_tag="VW049_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9433118.1"
                     /translation="LDVVGVQDFKKEFCIPNNKKIICYVERLDPEKRPDRFLEFAEKL
                     FLVREDVIFIMAGNGSMWAALKEKICHLKCRDNFRLLGEIYPATIVYQISDLLYIPSD
                     TEGIPMCVLESMSQGTPVLASNVGGLSEIIEHRVDGFLFEKEDVEGVCACANFLLNDS
                     EYLKYIGENSKSKIRKHESI"
BASE COUNT          203 a           53 c          132 g          199 t
ORIGIN      
        1 atttagatgt agtaggtgtt caagacttta aaaaagaatt ttgtattcca aataacaaaa
       61 aaatcatttg ttatgttgaa aggttggatc cagaaaaaag gccggataga ttccttgaat
      121 ttgcagaaaa attattttta gtcagagaag atgtgatttt tattatggct ggaaatggta
      181 gtatgtgggc tgctttgaaa gaaaaaattt gccatttgaa atgtagagat aatttcaggt
      241 tacttggtga aatctatcca gctactatag tttaccaaat atcggattta ttgtatattc
      301 cttctgatac agaaggaata cctatgtgtg ttttagagtc aatgtcacaa ggaactccgg
      361 ttttagctag taatgttggc gggttaagtg aaattattga acatagggtt gatggatttt
      421 tatttgagaa ggaagatgtt gagggagtgt gtgcttgtgc taatttttta ctcaatgatt
      481 ctgagtattt gaaatatata ggtgagaata gtaaatcaaa aataagaaaa catgagagta
      541 tatgatgaat tattagagaa gagtagtcat ggataggaat atagatc
//
LOCUS       DAVKGC010000224          551 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVKGC010000224 DAVKGC010000000
VERSION     DAVKGC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.224"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..>551
                     /locus_tag="VW049_002084"
     CDS             <1..>551
                     /locus_tag="VW049_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEU9433119.1"
                     /translation="CYHCSKMMVAETPLVKKNHQIPRIINQKIAQKLIEKISMTDIAH
                     QLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYAFTKGKMSFIAQDFDNLNIITVLE
                     GRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYDLAKQLFPCAKIVLDRFHIIQHLS
                     RAMSRFRVQIMNQFERKSHEYKA"
BASE COUNT          164 a          114 c           96 g          177 t
ORIGIN      
        1 gtgctatcac tgttcaaaaa tgatggttgc tgagactccc ctggtaaaga aaaatcacca
       61 aatccctcgt atcatcaacc aaaaaattgc tcaaaagtta attgaaaaaa tttctatgac
      121 tgatattgcc catcagctgg ccatttcaac ttcaactgtc attcgcaagc tcaatgactt
      181 tcactttgag catgattttt ctcggcttcc aaagattatg tcttgggatg agtatgcctt
      241 cactaaggga aagatgagtt tcattgcgca agattttgat aatcttaata ttatcactgt
      301 tcttgaaggc agaacacaag ctgtcatccg aaatcacttt cttcgctacg atagagccgt
      361 tcgttgtcaa gtgaaaatca ttacgatgga tatgtttagt ccttactatg acttggctaa
      421 acagcttttt ccgtgtgcta aaatcgttct agatcgtttc catattatcc aacatctcag
      481 ccgtgccatg agtcgttttc gtgttcaaat tatgaatcag tttgaacgaa aatctcatga
      541 atacaaggct a
//
LOCUS       DAVKGC010000225          508 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVKGC010000225 DAVKGC010000000
VERSION     DAVKGC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.225"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..284
                     /locus_tag="VW049_002085"
     CDS             <1..284
                     /locus_tag="VW049_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9433120.1"
                     /translation="SQAYPDDYLLLVMDNAIWHKSSTLKIPTNIGFAFIPPYTPEMNP
                     IEQVWKEIRKRGFKNKAFRTLEDVIQGLEKEVIKSIVNRRRTRMLFENR"
BASE COUNT          174 a           83 c           98 g          153 t
ORIGIN      
        1 tttcacaagc ttatccagat gattatcttt tactcgttat ggacaatgct atatggcata
       61 aatcaagtac cttaaagatt ccgactaata ttggctttgc atttattcct ccatacacac
      121 cagagatgaa ccccattgaa caagtgtgga aagagattcg taaacgtgga tttaagaata
      181 aagcctttcg aactttggaa gatgtcatac aaggactgga gaaggaggtg ataaagtcca
      241 tcgttaatcg gagacggact agaatgcttt ttgaaaacag atgagtataa aaagaaagtc
      301 ctcatttcaa tagaaatcac gactttctga tgaatttata gtaaaatgaa ataagaacag
      361 gatagtcaaa tcgatttcta acaatgtttt agaagcagag gtgtactatt ctagtttaaa
      421 tccactatat ttggggagtg atagaaaagc ccttcataag ccagtctact tgttcagatg
      481 tgagagcttt gacatccttt tctgtact
//
LOCUS       DAVKGC010000226          507 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVKGC010000226 DAVKGC010000000
VERSION     DAVKGC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.226"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..488)
                     /locus_tag="VW049_002086"
     CDS             complement(<1..488)
                     /locus_tag="VW049_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433121.1"
                     /translation="MVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQEYCQGQPY
                     GLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQSYLGGNTA
                     WIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAIFENLLDSY
                     LL"
BASE COUNT          180 a           76 c           83 g          168 t
ORIGIN      
        1 tgaagcaagt aggagtcaag caaattttca aaaatagcta acaatatgat tggtgtaaag
       61 tttttgtatt cacatgtcaa gtattctgag tatcgtgcag atgatatttc ttttatataa
      121 ctttttgaaa gcaagatttt ttcaaattca tcatctatta ttaatcttct atttccaatc
      181 caagcggtat taccacccaa ataagattgt gcaaatagca atatgtaaga aaatatgaac
      241 gtaaatactg aaagaggtag aattctatgc gtaaaaaaat agacgataat ggtagccagg
      301 agtaaagata tctctcgtga caatcgaatt cttttcagca tcttttttaa cagtccgtaa
      361 ggttgtcctt ggcaatattc ttgaactaaa ttaatcggaa tgacatctct caccatttct
      421 ggaaagtaga gtaagtttat aggttgaaat ctccagctcc catcgtaagt aaatggatat
      481 agaaccaaat acttaaaaga ttttttt
//
LOCUS       DAVKGC010000227          495 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVKGC010000227 DAVKGC010000000
VERSION     DAVKGC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.227"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            32..>495
                     /locus_tag="VW049_002087"
     CDS             32..>495
                     /locus_tag="VW049_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9433122.1"
                     /translation="MIQELDLTQSINKKVKTYSLGTKQKLALLLTLVTKPDILILDEP
                     TNGLDIESSQIVLAVLKKLALNENVGILISSHKLEDIEEICERVLFLESGLLTFQKVG
                     KDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEEGLRITMSGNIQSSELFKF"
BASE COUNT          175 a           59 c           95 g          166 t
ORIGIN      
        1 tgttgactac aatcaagaaa gatttagaag tttgatccaa gagttagatt tgactcagtc
       61 tattaataaa aaagtaaaga cctattcttt gggtacaaaa caaaaattag ctttgcttct
      121 aactctcgtt acgaaacctg atatattgat tttagatgaa ccgactaatg gtttagatat
      181 tgaatcatca caaatagttt tagcggttct aaaaaaatta gctttaaatg aaaacgtggg
      241 aattttaata tcgagtcata aattagaaga cattgaagaa atttgtgaga gggttctttt
      301 cttggagagc gggcttttga catttcaaaa agtaggaaaa gatagtcata atttcttgtt
      361 tgagatagct ttttcatcag ctacagatag agacattttc attaccaaac aagaatttgg
      421 ggatattgtt caggaagagg gactgagaat tactatgtct gggaatattc aaagtagtga
      481 gctttttaaa ttttt
//
LOCUS       DAVKGC010000228          482 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVKGC010000228 DAVKGC010000000
VERSION     DAVKGC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.228"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(<1..>482)
                     /locus_tag="VW049_002088"
     CDS             complement(<1..>482)
                     /locus_tag="VW049_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9433123.1"
                     /translation="LLLRLFPNDWLISLISGLISGIGAATIILLLRTWAVGIGKEKDR
                     VVTISLKRFSSSLGTSIGGILSGILLYCLIYFFHSMAYPIVLASASMLCLVTFFCIPK
                     IDSFVDKSERAERILESSNYMKKRIIFFGIFSGLIASIFSPYLPVILRRQEISEVFIG
                     "
BASE COUNT          187 a           91 c           61 g          143 t
ORIGIN      
        1 cccaataaaa acttctgata tttcttgacg tcttaaaata acgggtagat aaggagaaaa
       61 tatactagca attaaacctg agaagatacc aaaaaagatt attctctttt tcatgtaatt
      121 actagattct aagattcttt ctgctctttc actcttatca acaaaactgt caattttagg
      181 aatacaaaag aaagttacta aacatagcat acttgccgaa gctaaaacaa taggatacgc
      241 catactatga aaaaaatata ttaagcaata tagcaaaatg cctgacaata tccctccaat
      301 agaagttcct aaactgctgg aaaatctttt taaggaaatc gtaactactc tgtctttttc
      361 ttttcctatt ccgacagccc atgttcgcaa taaaagaatt attgttgccg ctccaatacc
      421 tgagataaga ccagagatta atgatatcaa ccaatcatta ggaaataatc tcaataataa
      481 ac
//
LOCUS       DAVKGC010000229          440 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVKGC010000229 DAVKGC010000000
VERSION     DAVKGC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.229"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            24..>440
                     /locus_tag="VW049_002089"
     CDS             24..>440
                     /locus_tag="VW049_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9433124.1"
                     /translation="MPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFV
                     GNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVRE
                     FLVNYYAHSKENGITGVTQKTINADEEYYNYCRKYFN"
BASE COUNT          160 a           47 c           91 g          142 t
ORIGIN      
        1 ttttagacga tgatgatgaa tatatgccag atcgaatttt aaagttgatg gcttgtttta
       61 aaaagagtag aatgaagaat ttagctttag tttatagtta tggaataata atttatccaa
      121 atggtacacg agaagaggag aagaccgatt ttgttggaaa tcccttgttt gttcaaatgg
      181 ttcacaatat agcaggtacg tcattttggt tgtgtaaaaa agaggtgcta gaattaatta
      241 atggttttga gaaaatagat tcacatcagg acggtgttgt tttattaaaa ctacttgctc
      301 aaggatacca aattgatata gtgcgagaat tcttggtgaa ttactacgct cacagtaaag
      361 aaaacggtat cactggagtg acacaaaaaa caattaatgc agatgaagaa tattataatt
      421 actgtaggaa atattttaat
//
LOCUS       DAVKGC010000230          433 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVKGC010000230 DAVKGC010000000
VERSION     DAVKGC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.230"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(1..98)
                     /locus_tag="VW049_002090"
                     /pseudo
     CDS             complement(1..98)
                     /locus_tag="VW049_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          151 a           82 c           65 g          135 t
ORIGIN      
        1 gatgagtagg gaggaaaagg taaaagttta tgcccaaact cttcacataa gaactctagc
       61 ttacccattc tatggaatct tgcattatcc attaaaactt ccagactcat acccttctaa
      121 aatctattca aaccacgtca gcttcacatt atatatgttg gttacctcac aagttttatc
      181 tgcaacctca aagctgttct ttgagcagcc tacagctagc ttcctagttt aatctttgct
      241 tttcactgag tatcaagtta aaaaaatgag gctggtcaaa aagtctttaa aattataaaa
      301 cgcatcatat caggtgtaca tgaactacac cccaaaagtt agacagaaaa aatctaactt
      361 ttggggtgtt tttattatga aattaactta tgatgataaa gttcagatct atgaacttag
      421 aaaacaagga tat
//
LOCUS       DAVKGC010000231          403 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVKGC010000231 DAVKGC010000000
VERSION     DAVKGC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.233"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(88..>403)
                     /locus_tag="VW049_002091"
     CDS             complement(88..>403)
                     /locus_tag="VW049_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9433125.1"
                     /translation="GAKDQLIKDELNNLTVQDDYRIQYMKEHLLQVAEALQDGVEIMG
                     YTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRYKKMSFTWYKGVIESNGESL
                     FK"
BASE COUNT          131 a           83 c           52 g          137 t
ORIGIN      
        1 tagaatttga tgtcttccaa acctctttat ttcatattta atgaaatgcc accttaaccg
       61 tgataatagc tagtcatcaa taaaaaacta tttgaataag gattctccat ttgattcaat
      121 cactccttta taccaagtaa aggacatttt cttatatcga ttgaatgtgc catttccatc
      181 atcatttcga tcaacataaa tgagaccgta cctcttagaa agctgtgcag tggacataga
      241 aacacagtca atacatcccc aagacgtata gcccataatt tcaacaccat cctgtagagc
      301 ttcagcaact tgcaataaat gttctttcat atactgaatt ctataatcat cttggacggt
      361 taagttatta agttcatctt ttattagttg atctttagca cct
//
LOCUS       DAVKGC010000232          402 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVKGC010000232 DAVKGC010000000
VERSION     DAVKGC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.234"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            80..376
                     /locus_tag="VW049_002092"
     CDS             80..376
                     /locus_tag="VW049_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEU9433126.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
BASE COUNT          139 a           53 c           80 g          130 t
ORIGIN      
        1 ttgaagagag ttaggaatac aacatgtgcg aattaactct caataaaaca tacataatat
       61 acaaaaggag tacactaaaa tgaaatctaa aagaatggaa tttcataata aatttcttat
      121 tgtttcagca atgttagcag tcattagttg gttaagctta ggtgttgttt cttttcctat
      181 gttggctggg acattaggaa tttcgacaaa agctgctgct actgtagtca atttgattag
      241 tgcttatagt acagttacag cagtaatttc aattgtaggt gcaatcacag gagtaggttc
      301 tattggttcg ggtattgcag caacagttct ttatatattg aaaaagaaag gtgctgctaa
      361 agcagctcta tggtagaact agatgaaaat actttctaaa aa
//
LOCUS       DAVKGC010000233          372 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVKGC010000233 DAVKGC010000000
VERSION     DAVKGC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.235"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            248..372
                     /locus_tag="VW049_002093"
                     /pseudo
     CDS             248..372
                     /locus_tag="VW049_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          141 a           65 c           45 g          121 t
ORIGIN      
        1 taagctcatt tcaaaattac gttaactttt ttcacgaaaa ggagaaaatt atgcgttacg
       61 attatggata gcgctttcaa atttttaaac tcctatccca tccttttatc tatattatga
      121 gcgaaaatat aataactgtc aagtaactaa agtgaatttt ataaaaaaat tacaagccaa
      181 atttgtaaag tttacactaa gccgctagag caatcgtcta tcagaatgtc cgtttatttg
      241 tcaataatcc gagaaaatct tgcaacgctt agaagtctat aaaaactatc aacatttata
      301 tgacttgcga atgacaattc tattaaacct ttccacactc tatctataca atcaagataa
      361 aaacatgtgt aa
//
LOCUS       DAVKGC010000234          371 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVKGC010000234 DAVKGC010000000
VERSION     DAVKGC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.236"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            <1..>371
                     /locus_tag="VW049_002094"
     rRNA            <1..>371
                     /locus_tag="VW049_002094"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           96 a           80 c          116 g           79 t
ORIGIN      
        1 agtttgatcc tggctcagga cgaacgctgg cggcgtgcct aatacatgca agtagaacgc
       61 tgaaggagga gcttgcttct ctggatgagt tgcgaacggg tgagtaacgc gtaggtaacc
      121 tgcctggtag cgggggataa ctattggaaa cgatagctaa taccgcataa gagtagatgt
      181 tgcatgacat ttgcttaaaa ggtgcacttg catcactacc agatggacct gcgttgtatt
      241 agctagttgg tggggtaacg gctcaccaag gcgacgatac atagccgacc tgagagggtg
      301 atcggccaca ctgggactga gacacggccc agactcctac gggaggcagc agtagggaat
      361 cttcggcaat g
//
LOCUS       DAVKGC010000235          358 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVKGC010000235 DAVKGC010000000
VERSION     DAVKGC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.237"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            complement(43..108)
                     /locus_tag="VW049_002095"
     CDS             complement(43..108)
                     /locus_tag="VW049_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HEU9433127.1"
                     /translation="MISKFFPILMLVMDIIIIVGG"
     gene            99..>358
                     /locus_tag="VW049_002096"
     CDS             99..>358
                     /locus_tag="VW049_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9433128.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTKTESFANLIQYIRKQ"
BASE COUNT          132 a           65 c           55 g          106 t
ORIGIN      
        1 gtctttagat actctaaaga cttaggtgta cacctaagtg atttaaccac caacaataat
       61 gataatgtcc atcactagca tcaaaattgg gaaaaatttt gaaatcaaaa cttggcatca
      121 cacttagaaa agttcgtaaa ggcaaacaaa tcagtctatg ctctgttgct gatgagcatc
      181 tttcaaaatc gcaaatatcc cgatttgaac gtggagagtc agagatatca tgtatccgac
      241 tgatcaatat tttagacaaa ttacacatta ctttggatga gtttcttatt ttacacgatg
      301 aagactatac aaaaactgag tcatttgcca acttaataca gtatatccgt aaacaata
//
LOCUS       DAVKGC010000236          349 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVKGC010000236 DAVKGC010000000
VERSION     DAVKGC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.238"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
BASE COUNT           97 a           66 c           55 g          131 t
ORIGIN      
        1 tcaacttcta aaacattgtt agaaatcgat ttgactgtcc tgatcgattt ctcctgttct
       61 tgtttcattt tactatattt ctctaaaaat gataaaggtt gagatttctc ctcgtaatag
      121 ataatcatgg ggatatttca atccaaagtt ttattcgtta tcacttgact attgcaaggt
      181 tttctagagc aacagagtca tggaatggac tcatggttga gatttctcct tgttgcttgg
      241 acttcattca aaagtctgtt acccaagcct tgttcaaact tccaatacac tagctgtttc
      301 catagcatga cttctgtact agactttctt ttccgaataa atagataga
//
LOCUS       DAVKGC010000237          341 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00061704
            SAMN33606307-rid17951033.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVKGC010000237 DAVKGC010000000
VERSION     DAVKGC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606307
            Sequence Read Archive: SRR23727503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722545.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:35:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 1,923
            CDSs (with protein)               :: 1,923
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 67 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 25 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606307-rid17951033.denovo.239"
                     /strain="AUSMDU00061704"
                     /serotype="16F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2042"
     gene            61..>341
                     /locus_tag="VW049_002097"
     CDS             61..>341
                     /locus_tag="VW049_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9433129.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYHPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAK"
BASE COUNT          122 a           66 c           49 g          104 t
ORIGIN      
        1 acagaatgta acagtgttct acgattttta ctagataaaa ctgtaaattc tgaaggaagg
       61 atcacttctt caacagaatt tggaaatttc gtaagtaatt tatcattcca acacggaata
      121 gctggactac tgtttcctct aaataaattg taccacccag aactggattc taaaatactc
      181 tctatcatca agaaggcagt gacaattaga acgacacaca catatgaata tcaatactca
      241 ctgctatttg gtgatgcagg ttatctatgg ttactcctac atttattttc tatcagtaaa
      301 aatcaatact atctacaatt agcaaacgtc accgctaaga a
//
